LOCUS       DAOPZK010000001        41593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_808_length_41593_cov_18.812557, whole genome shotgun sequence.
ACCESSION   DAOPZK010000001 DAOPZK010000000
VERSION     DAOPZK010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 41593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41593
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_808_length_41593_cov_18.812557"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKH68_00005"
     CDS             <1..260
                     /locus_tag="PKH68_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11346.1"
                     /translation="KDVINEALNETLSRTFSTSMSVLVVLFAIFLFGGDTIRGFIFAL
                     LIGSILGVYSTLFVAVPVAYDIMISRNKKKELKAANAELKK"
     gene            608..2182
                     /locus_tag="PKH68_00010"
     CDS             608..2182
                     /locus_tag="PKH68_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX11347.1"
                     /translation="MKKLLLVMFVLAVHFQLIAQTNFVQPTRLKADNFPGHVELSWKN
                     SPGFTYKILRSIDLPSKFIEIGETNQGQYFDFLGVSEKPSTVYYRIVPKGLPQKEAKN
                     ITSQIAVERKTMGDEDLLDMTQRYTTRYFFDFAEPTTGMARERSNDVNGDIVTTGGTG
                     FGIMSLIAGIERKYITPKQGQETIEKIVSFLEKTERFHGAWAHWYDADTQKAFSFSKY
                     DDGGDLVETAFLVQGLLTAREYFKKGSATERILANRITQLWETVEWDWYTQGTDSLYW
                     HWSKNYGWKMNHRIKGYDETLITYVLAASSPTYPIKASVYESCYKNSSYYFNGKSYYG
                     IKLDLGMEYGGPLFFTHYSFLGLNPTGLRDNYTNYFDRNKAHALIHRAYAIDNPKKQT
                     GYGPDLWGFTSSDDPKVGYTSHHPGTDDENGTISPTAALSSIVYTPEESMQVLKYLYF
                     QKGKQLFGKYGFYDAYNPGMVEGQQVVRSYLAIDQGPIAVMIENYRSALLWNLFMNNP
                     EIQKGLNLLGFRYTKN"
     gene            2201..5197
                     /locus_tag="PKH68_00015"
     CDS             2201..5197
                     /locus_tag="PKH68_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX11348.1"
                     /translation="MKNKSFILLLVGLIGISTLFAQQQQVKGTVVDAATGEQLIGVKI
                     LEKGTNNGTITNFNGEFTFAVPTGAPIVVSYIGYLTQELKATGTSLQIKLQPDTKLLD
                     EVVVVGYGTAAKRDLTGSIVKVSAKELSGRPNANPVSSLQGKVNGLSVVNSGQPGSEP
                     DIRIRGTVSKNQTKPLYIVDGIFNDNINFVNPADIESMEVLKDPSSLAIFGVRGANGV
                     IIVTTKKGAEGKVTVGISSSLGMKNIVGTPQMTDRTGFMTLYDEQRANQGSVPYPDYG
                     LFSGNSNWIDLISNNNALVNYNNISITSGTDKNKLYVGFGYLNEEGLIKNEKYNKFTF
                     TVNDELSLTKHLKLGVNVNGSKANLPQLHDFVSAINATPIVEAFNTENNVYNKLPDQI
                     GGPQIGNPLLFVEGNKFTSLANENRFVGNTFLELNFLKKFIFKVSYFADLGFNDGRSY
                     TPLYKVYNAESDQVVNFNSKSAVSQYRNTYTKYQQDYLLTYKDQFGDHGLTLLGGFTT
                     YFEDFNQISGAISQYAGGDPIPNDKRWWYLNVYPYGDPTTATNSSAEWERSTASYLFR
                     ALYNFKGKYNLNASFRRDGSSEISPSHRYQNFWALGGAWYVTEESFMQNQNIFDNLKI
                     KASLGQLGNQYTGIHYPYYPNYTTGSSTVFGEKVVPAYQLAYKNNPNLKWETVDAYEA
                     GVEMDLLKSKLHFEANYYDKRTKDLLTMVNDGANNFYMNAGSIKSSGFEFLGSWNQSL
                     AKDFGYTVTANLTTMNNKVLSVWKEGYKYYDGNSITEAGYPIGYFFGYQVDGVYQSYA
                     DKLASPVNTLGDYGPGDLKFRDVSKDGKITTDDRTMLGNPTPDFTYGISASLNYKAFD
                     FSVDFQGVYGNEVWRNWGNGSTFAVFNFRQARLDRWTGAETSNWEPKVGNEAINNLPS
                     SYMIEDGSYFRLRNMQLGYTFDKKLISKLHISSLRLYVSGQNLFTWKHNSGFTPEAGG
                     SATQFGVDNGGYPIPAVTTAGFNINF"
     gene            5341..6825
                     /locus_tag="PKH68_00020"
     CDS             5341..6825
                     /locus_tag="PKH68_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX11349.1"
                     /translation="MKNNKLFIVLLPFLLLMVAGCSDFLDRKPLGSATEGDLTVGGVE
                     GKVFGLYGNLRDDGMTGLPMVFMHVMRSDDAIKGSTVTDGAGYEQMADYFKYDKADGW
                     LTLGYWNDHYKLIYACNDILHDLDSLKLNSDGDMINQAEAKFFRAYAYFDLVRTFGEV
                     PKIDFKIYNIGDANIQKSTVPQIYALIDQDLDFAASHLPSGWVSTYTGRVTSGTANTL
                     WAKTKLYRKDWAGALAKCELVINSGKYSLLDSYEKFFTEKGENSSESILEVQQYVSAN
                     GSVSYSNNCNGAQGVRGSGDWDLGWGWNVPSKALVDSYESGDPRKTTTILESGGPDGY
                     GLTVPASLATIQPYWNRKVYSDPARRSSSGIKYANWLNIRLLRYADVLLMAAEAANEL
                     GGTPNTTKALAYVEMIRARARGTANVLPKVTVTDQAALRTAIKKERRAEFAMEYERFF
                     DLVRWGDAPEILGAAGYTAKCQYYPIPQSIIDKAQGKLTQNPNW"
     gene            6854..7729
                     /locus_tag="PKH68_00025"
     CDS             6854..7729
                     /locus_tag="PKH68_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNX11350.1"
                     /translation="MKKILNRLIAFTAAILLLSGCQDMNRPVLGDYPKDTNPVGGPLK
                     FYVAFDGTTTDPLMNAVDSIKANFATANTTTSIDGVRGKAMQGAAGKFVSYAKPNDFV
                     SKAGSFTISFWEKHDGQTKNNAMGNGPEYPFSFVSPSAYHWSSSNMFLLFEGNNTACA
                     VKFVVVSGVSPDGNSSTADTWLTWEGGASIPGLLDNTWHHCAFVYDATTSGLTFYKDG
                     VAIGTKTWAGHGPIGLVDSKVNSVRIGAGPQGNAGDTSDDWLRSSWKGGLDQFRMYGT
                     ALTAAEVAALNSGKK"
     gene            7808..10171
                     /gene="bglX"
                     /locus_tag="PKH68_00030"
     CDS             7808..10171
                     /gene="bglX"
                     /locus_tag="PKH68_00030"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNX11351.1"
                     /translation="MKLKVLILALCCLIIPSLPAQKTTKKTAKKIVTEQVEPVLNPDK
                     SLYFTNDPKMDKFIDELLAKMTLEEKIGQLNLPSAGDFTTGQVQSSDIGKKVLEGKVG
                     GLFNIKGVDKIRDMQRTAVEGSRMKIPLIFGMDVIHGYETTFPIPLGLASSWDMDLIR
                     KSAQIAAAEATADGINWAFSPMVDVSRDPRWGRVAEGAGEDAYLGSRIAKAMVNGYQG
                     NDLKANNTMLACVKHFALYGASEAGRDYNTVDMSKVRMYNEYLPPYKAAIDAGAGSVM
                     VSFNEIDGIPATGNKWLLTDLLRNQWKFPGFVVTDYTGILEMIEHGMGDLQQVSKLAL
                     NAGVDMDMVTEGFLLTLTKSLKEGKVTYSDIDLAARRILEAKYRLGLFDDPYKYCDSN
                     RAKTEIFNSQNRAEARTIAAQSMVLMKNQNNTLPLKAAGTVAVIGPLADNPENMPGTW
                     SVGAKHSQAISLMKGLKETLGDKVNFVWAKGANFDYDPDFESRVSMFGKNSYRDNRPK
                     EEILKEAMDAARKADVILAALGESAEMSGECSSRTNIEVPQSQKDLLIELKKLGKPIV
                     LVLFTGRPLVLNDENKLADAILNVWFAGSEAGYAISDVLFGKVNPSGKLPMTFPRNLG
                     QVPIYYNHKNTGRPEIKEDQFDKFKSVYLDVPNSPLFPFGYGLSYTTFSYGDISLSNT
                     QLQGNQTLKASVTLTNTGKYDGAEVVQLYIRDVVGSNTRPVKELKGFQKVMLKAGETK
                     TVTFDITPELLKFYNNKLIYDWESGDFEIKIGGNSYDVKTATVNWMK"
     gene            10234..11895
                     /locus_tag="PKH68_00035"
     CDS             10234..11895
                     /locus_tag="PKH68_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNX11352.1"
                     /translation="MNKIVVIGFLCVLAMFSCKKEEPKPPVVKTRVLRIISVQINDQT
                     WADNSVIYNVSVVGKPVIKITFNDTIDVSKFVASKASFSGDIGQNFTVSKGTDTKTLL
                     IMLTVLPRDLTAYTFNLDSGENLGGSIYASFSSVFVTQLDTTPKFPTISTDSLLTLVQ
                     KQTFRYFWDYAHPVSGLARERLGSGETVTTGGSGFGLMAILVGINRGFITRNQGFEQI
                     NKIVNFLNKPETDKFHGAFPHWMNGTTGKVIPFSTKDNGADLIETSFLMQGLLAVQAF
                     FKSGNSSEQAMCAIIQQLWEKVEWSWFQKNGEQKLYWHWSPNYQWDMNMPITGWNEGL
                     ITYVLAASSPTFPITKSVYDNGWASNGAMKNGNSFYGVTLPLGEELGGPLFFAHYSFL
                     GLDPRYLSDTYANYFTQNQAHARINYNYCVANPKGYTGYSENCWGLTASDFPDGYTAS
                     SPNNDQGTIAPTAALASFPYTPAESTKALNFFYYTLGNRMWGEYGFKDAFSLTRRWFA
                     DSYLAIDQGPIIVMIENYRSGLIWNTFMNHPDVKSGLTKLGFIYQ"
     gene            11992..12411
                     /gene="mscL"
                     /locus_tag="PKH68_00040"
     CDS             11992..12411
                     /gene="mscL"
                     /locus_tag="PKH68_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNX11353.1"
                     /translation="MGFVKEFKEFAIKGNVIDLAVAVIIGGAFGKIVASFVADIVMPL
                     IGLLLGGVNFTDLKWVIKPAEIVNGVETVAAATLNYGNFIQVTIDFLIIALAIFIAIK
                     GMNSLKKKEEPAPEAPAAPSNEEVLLGEIRDLLKAKK"
     gene            12644..13177
                     /locus_tag="PKH68_00045"
     CDS             12644..13177
                     /locus_tag="PKH68_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11354.1"
                     /translation="MNKLIVLLGVLLTVLTGCTEAKKLTQFDLPVNQEFTLPALPIVA
                     TNTEFTTPEFETNYLSYFETFNINTDLVESIGIKSVDFNITAPETGDFSFLQSVEVYI
                     SAEGLNQVLLASKKNITNAGKTLSLDVDSKVDLKKYVTKNNIKLNLIVSTTKTTTAKY
                     TIDTKLVFLVDANVMGL"
     gene            13310..14239
                     /locus_tag="PKH68_00050"
     CDS             13310..14239
                     /locus_tag="PKH68_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11355.1"
                     /translation="MKKTAGFFFCIFFCLPLSAQIHAADSTVQVAGHWHMHDKQNYHV
                     TEESNVIKNEADTINAAKITYQLDIEILDTSEDSYTIQWLSHDYRIIKATDRQLIPLY
                     KLGENSRIVFRTTETGKFKEIVNGDELLEHNKKEFELLVSQYDTIPAVKQWIDKFEDR
                     YTTRETIETTAARLINQFYVFHGANYKLGEEVNSQVKLSNPYSGIPFDAVMTVSLDEM
                     DALNDYSILRMWQTANSAQLSQFAQNLIKEVAREMNVDVKESLQNISPMINETRIASQ
                     IHGSTGWIIYSTQTTEVSLDNVLNLEEITIELQ"
     gene            14257..14733
                     /locus_tag="PKH68_00055"
     CDS             14257..14733
                     /locus_tag="PKH68_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNX11356.1"
                     /translation="MTKYRFIISLICFGIFIIGYSQKFDARLAKKLGADEYGMKSYVL
                     VILKTGTNTTTDKQTLDSLFRGHMDNIQRLSAQGKLIIAGPMKKNPNNYRGIFILDVK
                     TQDEAHQLLSTDPTIREKIFDVELYDWYGSAALPTYLPAHTKIEKKSINNNTLNLK"
     gene            14743..16134
                     /locus_tag="PKH68_00060"
     CDS             14743..16134
                     /locus_tag="PKH68_00060"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNX11357.1"
                     /translation="MHQKLFFLITLFTGVIFSSCKTIQPEAPKESYLPTEITVAYSEL
                     PIHMELDVKKLEKAVNTSMNGLLYEGSNITDKDLSVKIWKTADFTFNINNNAIEYRVP
                     LKVWSQFAWKVEKLGLSLADDYEATGTIALKYKTVISIDRNWNLVAQTTSQGYEWIET
                     PKLNIIGVNVPVKPIANLAMAKYEKMITQQIDKSLAEFADLKKQVTDAWAELQKPVQT
                     SVENDIWVRIIPKDILVSPFATKAGKLNMDIALYGKVETIMGAKPAAGKPVALPEFKW
                     INRPPQQFNLNVSTDVTFDKISQLAKEQLLNKTFTEGKKHIQITDLSVFGSEGKAVFS
                     ADVIGSIKGRIYFTGEMQYNPAKMTVEIINPGFELKTRNALLKSADWLLHGLILKKIV
                     PYLSYPVKEYTDDMKAEANTMLQNYQVREGINLQGKLNELTVKSVNLVPGAIRIQANL
                     KGNVMIKLENMKF"
     gene            complement(16148..17479)
                     /locus_tag="PKH68_00065"
     CDS             complement(16148..17479)
                     /locus_tag="PKH68_00065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX11358.1"
                     /translation="MQFSELNLNTPLINALNDLDITRPTTIQEKAYPVIMSGRDMVGI
                     AQTGTGKTIAYLLPCLRQWKFTQEKHPQILIVVPTRELVVQVVEQVQKLTTYMTIRVA
                     GVYGGENMIRQAPLIFDGLDVLVATPGRLLDFALNGTLKLKLVKRFIIDEVDEMLNLG
                     FRPQLIRVMDLLPKKRQNLMFSATMTPEIQNFIADYFIDPLHIEAARTGTPLENIKQT
                     GIKVPNFNTKINLLEFLLKTEPGFSKVLLFVSTKKLADDVYNRISAIFPETVGVIHSN
                     KSQNNRFEALKSFNDNKLRVLIATDIVARGLDFDDITHVVNFDIPDEPENYMHRIGRT
                     GRVDKKGVAVSFITKDDTEYRESIQQLMGKKIPMMRMPEDVVVSTELADCEIPQIQNG
                     NSVAFIAKREIHGPAFHEKKDKNKKVNMKVRRKDALKQKYKKPKTRGQKKK"
     gene            complement(17655..18485)
                     /gene="tatC"
                     /locus_tag="PKH68_00070"
     CDS             complement(17655..18485)
                     /gene="tatC"
                     /locus_tag="PKH68_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNX11359.1"
                     /translation="MGKPDSEQDFTFWEHLEALRWTILRSLGVVTLLLFVFFGFKDYV
                     FSNIILPPLNSDFYTYRLFCLLGEKLKMPGLCPDDFRVQLININLSGQFVTHITSSLI
                     MAVVVSMPYLLFEIWRFINPALYANERKNVSLIFITSSFLFYLGALVSYFLIFPLTIR
                     FLGTYQVSDLVTNQISIQSYMNALAILIFSLGIMFELPIIILALNRIGLVSRKALKTF
                     RKYAFVTILIVAAVITPTTDPFTMLVVALPIYLLYEVSILVCKKDLPESEEEAETDED
                     "
     gene            complement(18535..18735)
                     /locus_tag="PKH68_00075"
     CDS             complement(18535..18735)
                     /locus_tag="PKH68_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNX11360.1"
                     /translation="MTGNILLFSLGAPEVILIALVVLLIFGGKKIPELMRGLGKGVNS
                     FKKGLKDVDDEIKNDLKDETDK"
     gene            complement(18879..20267)
                     /locus_tag="PKH68_00080"
     CDS             complement(18879..20267)
                     /locus_tag="PKH68_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNX11361.1"
                     /translation="MDSDEVGLKFMYAILDVVLLNVAIAIVFSLSPVFTQMNLHDIGL
                     LYLHGNISELIAYSLYSKRNYYFRDRFVNRVKYLSYRMIIFVIALLILAQIFLHKPTP
                     YLFLLKYTALFYAIKLVAFYLIYEIYKYRRKKGLSVHRVLILGLNRTGVILGDLIKNN
                     PELGLNLVGFISENHDIKKCDKKILGSYDDLERLTDELNIDMIFVTLPKYYDTNLTRE
                     LLALCNKIGVRVRYVPVNQAWFKARVKNTEAVGSFMMFNPQEIPLDRLTYRAGKRLFD
                     IGFSLLVVILLFSWLFPLIALIIKLESKGPVFFIQRRTGINNKTFYCLKFRTMRTNLD
                     ADTRQATKDDDRITKVGKLLRKTNIDELPQFLNVLKGDMSVVGPRPHMLKHTKQYSAL
                     IEHYKVRHFVKPGITGWAQVNGYRGLTDELWKMEKRVQFDMEYLEGWTFIWDLRIIMS
                     TLFSKSIYKNAG"
     gene            complement(20362..21165)
                     /locus_tag="PKH68_00085"
     CDS             complement(20362..21165)
                     /locus_tag="PKH68_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNX11362.1"
                     /translation="MLQDYTIFNQSLNEISQPKALIGALNAHSFNTVQKDEDFHKALQ
                     SCDVLLPDGVSIVMAMRMLTGKKIKKIAGEDLFLYEMERINRSGGKCFFLGSSENTLR
                     LIQNRAATEYPNIQVRSYSPPFKHEFSDEDTHTMIDAVNEHEPDVLFVGMTAPKQEKW
                     AYSNFHHLKAGHVCCIGAVFDFYAGTVKRAPAWMIKIGLEWFYRLIKEPRRMWRRYLI
                     GNVKFIAYILREKMLFSTGNIKQEELQFDSKLLINEEVPVQDINKKDLK"
     gene            21598..24885
                     /locus_tag="PKH68_00090"
     CDS             21598..24885
                     /locus_tag="PKH68_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX11363.1"
                     /translation="MLLLFELLNRKEFLLLSTRIAMNKITSHPILDVPDGGETSFQFE
                     GKTIVCDKGITVAVALHRAGFSVHSHSLDNRSRSLQCGIGKCGACEMLVDGEIRRICI
                     TGVDGVREVREVPRDYLPQTKQHDPTKSVSVYKTTVAIIGAGPAGLACREELRKHNIE
                     NIVLDNNNKIGGQFVMQTHSFFFFEKEKKFGGMRGFDIAKKLAGDNEEGILLNSTVWD
                     ILDGKRIAAKNISTEEIFYVDADYLVVATGAVPTMPVFENDDLPGVYTASVIQKMMNT
                     GFTLPGKNVLTVGAGNVGYLTSYQLMQAGARVKAIIEAQPVEGGFPVQADRVRRLGIP
                     IITSHRLIKAIPNDTLTGITGAVIAECKNMEIIPGTEKIIDDIDTINICTGLTPDNLL
                     ITKGNEVFGRKCFGAGDAIHIGEGTTAVLYGKKTALDILQEMGVKINYEEYLSLTEEF
                     IQSQHHPARVIRQPYLPTPGRMSEKPFVLIDCLHSFACNPCAFACPHEAITKSTTNSV
                     PQIDFTKCTGCMECVHQCPGLAIFGYAPKNKLIYIPLEFLAEENETVFLVNNTGEKLG
                     EGVIVKILPKPNKTNIAVVKALNLNEEELLQVRGFIAKKNYPEPLELKPVEFKEEQKI
                     YLCHCEDVTIEKVLSAVGNRKFISIGEIKHITRLGAGVCRGQLCTDRLKSVMKEKGIT
                     LTGDAAPRAPLSNQLSLGELITPTKKEKIVTGKNIRKIEVPVLIAGGGMAGSSLYRYF
                     AESGYKPALINSGRGSSWRNIAAGRAVFGIPEIADIARHNLEIFKELQQISDIHFRST
                     RFVNFIHNETLYEIMEKSQSWSGGQIIECEDFTKTISPFVNPDLKKNYLAAFITPDCW
                     QATPGLVIDLIRSLGSRAGGTIYEDCELMEVHKENDKFIALVKDNESNYIEFYTPHFV
                     NALGADAEKFAAQLGLETGLYPVKQQAFITRRLPNLGINNEPLDMLIDRTKVNGFTNF
                     YAQQLLETGQIIGCAETASEPADANKNIRINTQDFIRSVSEKLVNWIPRLSSVGLQAV
                     WAGYYVEPRMIVDPENGLLAGLQGSGFMFSQYLAKMYVDKLSGKKVPDYFDRLKLDGD
                     GFKRKNI"
     gene            complement(24958..25890)
                     /locus_tag="PKH68_00095"
     CDS             complement(24958..25890)
                     /locus_tag="PKH68_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11364.1"
                     /translation="MLTETSKETYRSHFTEDPHPYITEGFIDLVKQKTEKVVHLITNE
                     GNISLGLIAGLKDNELSSPFSAPFGGFHYTHDDVYYSEIFNFLAQLKEYAVDQHLNKM
                     SFILPPNIYNSSINAKFVNAFVRQGYTLEIPDLVSWIDLNRFNGTWKRKRIAENCKSA
                     IKNNLTFSLVTDEKSMKESYEVIVNNRTQLGREIFMSFDDLMDVNRLFPVDFFLVKNK
                     EGDNIGAAIFYRGHPKIVQGIFWGDIIPRTSIGIMDFLVFNLFIHYKNLGFDYIDLAL
                     SSKSGIPNEGLIRFKETHQCTSSLRYTITWTPDI"
     gene            complement(25908..27032)
                     /locus_tag="PKH68_00100"
     CDS             complement(25908..27032)
                     /locus_tag="PKH68_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX11365.1"
                     /translation="MSEKKNILFLLHVPPPVHGSSMVGKAIADSELINSTFRCRYLNL
                     LMSRTVSETGKPGALKVFRFIGLWFRVLGEIIRKKPDLCYLALTVKGTAFYKDIFLVT
                     LLRIFSIKCIYHLHNQGVKQNETKKINYLLYRFVFRDSHVILLSRQLYRDVETFVPSS
                     RIFCCPNGIKDTLTNVETHTDSGQKTVKILFLSNLMHSKGVFVLLRACSMLKQNGVDF
                     DCSFIGGLGDIDVDNFNNYLNENELTDNVKYLGQKFGIDKHRAFTETDIFVHPTMNDC
                     FPLVLLEAMQHQLPIISTYEGGISDIVDNDKTGFLIPKNDAEALAEKLEILIKNQDMR
                     LKMGKAGRHKYEKEFTLDIFEKKMNSILNQTIEQNLFTTY"
     gene            complement(27025..28152)
                     /locus_tag="PKH68_00105"
     CDS             complement(27025..28152)
                     /locus_tag="PKH68_00105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX11366.1"
                     /translation="MIKIFLLIASIKLGGSENVALSIAEHCKLARQDDFEFVIVELFP
                     TRDAYALNKKQKLVSQNIRTITLAKTPKRMSLLPGPLRLVYFLLKEKPQIVHSHTDLP
                     DLVLSTTLRILSFFGIKIPKIIRTIHSTQLWPTHFNLGKYTEKAFSDEWICGVSEPTL
                     KAYNELRRKYHFPVSVHQQIINNGCIIPDRKPHPFNIDTAKINIAFCGRFEDYKGMDV
                     LIPCIQMIYALYPGDFLFHIIGSGTFQHEIDKLANDYDTVLVYEAVPDIAAKLYDFDY
                     LFMPSHFEGLALMSIEASFAKIPVIATFASGLKETLPHDWPLMFHKENREELMAVFEK
                     IKNKSYDYEKLKNQAFRFVNEHFSMNKMIDTYAELYRRINE"
     gene            complement(28145..29275)
                     /locus_tag="PKH68_00110"
     CDS             complement(28145..29275)
                     /locus_tag="PKH68_00110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX11367.1"
                     /translation="MAKKKLLIFHPALAPYRVDQFNALSLLFELEVVFLYDNLWSYKM
                     DQSRLISQLNFRVSWLLTGPRYKGRVFRYGMLKKIKSINPDIISGYEFSFTTQYLILL
                     KRLGLIRQKIGSMIDDSIDICHHVQSGIRAKARKYSVKYMDFLVVNSAEVSLFYQKEF
                     QLHENQLIISPLLQNPERLRSNSEKLEEIAKTYAEKYQLNGKKVLLFVGRFIPEKALP
                     QFLRVICNKLHEQSNCVCIVVGEGPELRSLKAIILEKNLEEKVILPGKFEGEELYAWY
                     LCASGLVLPSVSETFGAVVNEALIFGLKVLCSQYAGASCLIRPDNGLIFNPLNGDDTI
                     EKLTLLLAEIEPVGDADLTKKPSLMRNSEQQFRNEWRKLTHD"
     gene            complement(29311..30183)
                     /locus_tag="PKH68_00115"
     CDS             complement(29311..30183)
                     /locus_tag="PKH68_00115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX11368.1"
                     /translation="MEPQHIAVLLTCHNRKAKTLGCLTSLYAAELPVGYDMEVYLVDD
                     GCTDGTADAVSAQFPSVKIITGSGQLYWAGGMRLAWKTAMKVKNYDAYLLINDDVTIN
                     TGFLHHLIHTDKFSMDNYGMKGIYTGATKEKNSEKMTYGGTIIKSNHFILKTEFLKPS
                     DSPQVCDLTNANILWVSEEVLGKIGMFDPRFTHGIADYDYSLRARKMKIPVLQAPHIC
                     GVCDDDHGHNWKPREVPLKERIAYLKSPTGLAYQEYLYYIRKHFPLYLPYSFVLLWLK
                     VLFPSLWNKYKIDK"
     gene            complement(30183..31490)
                     /locus_tag="PKH68_00120"
     CDS             complement(30183..31490)
                     /locus_tag="PKH68_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11369.1"
                     /translation="MQLTKKQLIKLLLPVTILLVAVYSVLSFPNEKLPLLTYLKSTTF
                     WWVISVAVLIVLLMSNFYFFDRKNAAHLRIVEIYLLWNIICIVRGMFVADGYWEWKGL
                     IGNAFALLLPVVAYSATNKVMVQYVLSFYVKIGLPLFLIFALLIRTDAYGFYLIPVSF
                     LLLFLPAFSLRQKIILIFFTAVVLTADLGARSTIIKFAIPLIFLLVYYFRNTLSVKTM
                     EIIRLALLILPIFFFVLGITGIFNVFKMDEYIKTSYEGTGVDEQGNRVKIDLKTDTRT
                     FIYVEVLQSAKKNNYWLFGRTPARGNESEAFGDILFELTGKYERLENEVAITNVFTWT
                     GIIGVILYFWIFFSASFLAVNRSENIYAKIIGLYVAFRWLYAWVEDVNNFSLNYLMLW
                     IMLGLCFSFTFRGMTDREVTIWVRGIFDKRYLNPENDTKKNEE"
     gene            complement(31530..32810)
                     /locus_tag="PKH68_00125"
     CDS             complement(31530..32810)
                     /locus_tag="PKH68_00125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX11370.1"
                     /translation="MRILWITHDVFEPFLPYLKGKPTQGASWTTPLFYNLRDVEQVKL
                     GSIAPVTDGEEQKMEIENVTYYSIPVAKNDNMTGMQKKLADKYISAIRDFKPDIIHIH
                     GIERNFGLLRQFVDPQIPIVCSIQGIIQPCLEYMKCSVANLNLNHYKSIKNRIGRGGV
                     NAAIRSWEKYSVIEKLIFEINEYFIGRTSWDKAQLAAFNPAAHYYHGEELLRTPFYSQ
                     TWDIKTCQRHRIFISSVAEPLKGFHVLLKAADILKNKYPDLRIAVPLATFRQKSSGIW
                     DFLFTEDYANYLKKEIKSLELGENIIFYKRLSAAEMADEYHKAHVFTLTSYAENSPNS
                     LGEAMLVGTPTVVSPVGGVMSMVKNEESSLFFPSGDAAMLAFQIDRIFSDDELAVKLS
                     QEAKIIALKRHDIVETTNQYMNIYAEIIKNHAVK"
     gene            complement(32850..33764)
                     /locus_tag="PKH68_00130"
     CDS             complement(32850..33764)
                     /locus_tag="PKH68_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX11371.1"
                     /translation="MKNTLQPLVSIVIITYNSSAYVLETLESAKAQTWKNVELIVTDD
                     CSPDNTVEICKDWIDKNKDRFIRTELITVPVNSGISLNCNRGIKASAGEWIKIIAGDD
                     ILKDNCIEDLLNFAGENPEADFIISDLEEIDNQSRPLLHKTTQDTGLRHFLKGKTAQE
                     QLKNYARWPEFLNSPTFFFKKETMAAIGYYDDEFKIYDDMCLIFKINAFNKKIFYLDK
                     STVKYRIHEHAISRSSSKKINDKRDKEAYEIFKKYRKPHLSLLNPIDLSVYYECWLYY
                     RYKGFFGHKGIAVLNKFSLFYWYSRCFV"
     gene            complement(33770..35266)
                     /locus_tag="PKH68_00135"
     CDS             complement(33770..35266)
                     /locus_tag="PKH68_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen translocase"
                     /protein_id="HNX11372.1"
                     /translation="MNTQQSSYRQIMAATSLFGGVQVFQILIQIIRSKFVAVLLGPAG
                     MGIVGLLTSTLGLISGLTNFGLGTSAVKDIAEARTTENETRIATVTIVLRRLVWISGT
                     LGTIVVLAASPWLSRLTFGNSEYTLAYIWISISLLFNQLTSGQLVLLQGMRKLQYLAQ
                     ANLFGSLLGLLITVPLYYKWGIDGIVPGIILTSVTSFLIALFFSNKISLQKVRVSKVR
                     TIAVGRNMVTMGFMISLSGFISLGVSYLLQIFINRHGGVADVGLYNAGFTLINTYVGL
                     IFTAMATDYYPRLSAIAHNNLLCKETINQQAEVALLILAPIVVGFLVFINWAIILLYS
                     SKFIAVNTMVYWAALGMFFKAASWAIAFIFLAKGTSKLFFWNELSGNLYMLILNVSGY
                     YFFGLTGLGISFLLGYLVYLIQVFVIAKLKFEFSFNSDFLKVFIIQFSLALSCFLAVI
                     SFQQPYPYIIGTVLLLISGGYSLNELEKRIGIKEIIKEFTHRNKTTDS"
     gene            complement(35293..36399)
                     /locus_tag="PKH68_00140"
     CDS             complement(35293..36399)
                     /locus_tag="PKH68_00140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNX11373.1"
                     /translation="MIKFLDLQKITQKYSAEIHEAVAGVIDSGWYLTGIENQKFEAVY
                     AGFIGTKYCIGVANGLDALRLILRAYIEMGLMNTGDEIIVPANTFIASILAITDNGLV
                     PVLVEPDINTYQIDDRKIEDAITPKTKAIMIVHLYGQCAYTDTIGSICKKYNLKLIED
                     NAQAHGCKFNGITTGSLGDAAGHSFYPGKNLGAFGDAGAVTTNDDQLSEVVRTLANYG
                     SKHKYIFDYQGLNSRLDEIQAAILQVKLRHLDKDNERRKEIARYYIENITNPAIITPE
                     VKDWDANVFHIFPIRCGQRDELQKYLAEKDVQTIIHYPVPPHKQACYREWNNLSFPIT
                     EQIHREELSLPISQVLTDAEAEMVVKIVNSFSVT"
     gene            complement(36399..36926)
                     /locus_tag="PKH68_00145"
     CDS             complement(36399..36926)
                     /locus_tag="PKH68_00145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX11374.1"
                     /translation="MISHLSDVLSTKIGKNTKIWQFCVILPDAEIGENCNICAHVLIE
                     NKVKIGNNVTIKSGVQLWDGITIEDDVFIGPNATFTNETVPRSKTYDHTKLKKTLIKK
                     GASIGGNATILPGVEIGEYAFIGAGSVVTRNIPRNTLWYGNPARQKGFITNDGILLDM
                     HKKDKDGNFHELNSM"
     gene            complement(36923..37339)
                     /locus_tag="PKH68_00150"
     CDS             complement(36923..37339)
                     /locus_tag="PKH68_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNX11375.1"
                     /translation="MRKTTVYDCSIIEIDKHHHEKGNLSVIENGKTIPFSVKRVYYLY
                     DVPGGESRGGHAHKELKQFIVAVSGSFDVKLDDGFQKRTYTINRPYEGLLVVPGIWRE
                     LDNFSSGSVCLVLASTEYSAEDYIREYDKFIEYKNI"
     gene            complement(37336..37743)
                     /locus_tag="PKH68_00155"
     CDS             complement(37336..37743)
                     /locus_tag="PKH68_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNX11376.1"
                     /translation="MQTLRGARIIDLPKTLDRRGNLSVIEELKNIPFKIKRTYWIYDV
                     PGGEVRGGHAYKVNQEFIVALSGSFDVILDDGKERKTFSLNRSYYGLYVPKGVWRQMQ
                     NFSTNSLALILASAKYDPDDYIFDYELFKNSVL"
     gene            complement(37788..40226)
                     /locus_tag="PKH68_00160"
     CDS             complement(37788..40226)
                     /locus_tag="PKH68_00160"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX11377.1"
                     /translation="MNKYSEKEDEFNLLPEPPIDLLGILIKYISYWKWFVLSLIVCVA
                     VAATYLKFTLPSYGIKTSVLIKDDVKGSGSVEMSVFKDMSIITQKNSVDNELELLKNS
                     LIFEKVVRELGVYATYYQISTLSLIQKLGINKLIPGLGKYRSYVLYGDESPILVTLPD
                     SILKNFNEPVSFQVLVHPDGKCEFTGTYNENPYTVNTIISDKDVKLPFGRINLRKSNL
                     KLHDDMQIEVNLENPMTIADQFLKQIKMELASKGSSVVNLSMVSSNVALGKDFLRKFI
                     EVYNREEMNDQLEMANKTSQLIEEHLNSLSKELNTVESQAENYKQSQGITDLAAQSDI
                     YNAQTADAQQKRLELETQLGIVSNLKSYVQNNQSHDQLLPANSGIKSSGLNDMISNYN
                     KLVLDKNRLSRIASGSNQAMVDLNNKIENTYNTVKSSLENEENNLQIARRDILSQYYQ
                     TSARIRAVPRQERQYTDIKRQQGVKEGLFLYLLQKKEEKYMNMSAVAPSTKLIDTVRY
                     AGPVAPRKKIILFSSLLIGLLIPVIGIKIRDLMRYQIENKEELEAFSTVPVLGEIPKT
                     DQTGNVLVKENSTDSFTEMVRLLRANVLFVMDDPDKKVINLLSSVSGEGKTFVTINLA
                     ISLALLDKKVLIIELDIRKPKLSEYMGIENKTGITLYLSGQISQSELVKPTGIHPNLS
                     IITAGTIPPNPNELLAKPALDKLIDDLRQNFDYIVIDTAPIGVVSDSFLLNRLTDVTL
                     YVVRAEFTPKKDIEDATILYKKKKLKNMYFVLNDVDLNKHTYRYGYGKKYGYGYGYGK
                     TRHTYGYESETKKS"
     gene            complement(40281..41108)
                     /locus_tag="PKH68_00165"
     CDS             complement(40281..41108)
                     /locus_tag="PKH68_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX11378.1"
                     /translation="MNKTTYKNMFLLAGILFISSCSGVKNIAYFEKSDNKSKLSVNTN
                     PSSGLFDARIKPKDMLSITVVSSEPEASRIYNLVTPQTETPTDAGSLYSQPKLQNYLV
                     DNDGYINFPVLGKIQVKNLTRKELELTLQKMLAPAFTKEMPIITIRINNYSVNILGEV
                     LKPGKYETGNDRMTILEGLALAGDMTIYGRRDNVKVLREDAEGIKKYITLNLNDKNII
                     NSPAYFLEQNDVVYVEPNKSKSNSANFGAAETFRLSSLSVGLTLTSLVLTVYSIYKK"
BASE COUNT        12355 a         8590 c         8559 g        12089 t
ORIGIN      
        1 aaaaagatgt gataaatgaa gccctgaatg aaacattaag ccgtactttt agtacttcga
       61 tgagtgtgtt ggtggtatta ttcgctatat tcttattcgg tggcgatacc attcgtggat
      121 ttattttcgc cttgttaatt ggttctatcc tgggagttta ttctacgtta tttgttgctg
      181 taccggtggc ttatgatata atgatctcaa gaaacaagaa aaaagaactc aaagcagcaa
      241 acgctgaatt aaagaaataa ttctcagcat aaatagatta gtaattagta ttttttataa
      301 agctgaaaag ccctgtagta atttactaca gggctttttg ttttttactt cttgtagtga
      361 ataaaataat tgcaatatta tacatattat ataaaatatc aatagatttg catttacaac
      421 atcgaacgat ccaaaccatc atcaataatt ttacccgaat aaatgtggat ggttgaacac
      481 agtagcggct gaatttgtta taaaaaagta ttcggaaatt gcatgtcagg cgctcttgta
      541 caagcacaga agaaaaccgg tgattttttt tttcaaaacg aaataataca ttaaataatt
      601 gatcgatatg aagaaattat tacttgtaat gttcgtcctt gctgttcatt ttcagctaat
      661 tgcccagact aactttgtac agcccacccg attgaaagcc gataactttc ccggtcatgt
      721 agaactcagc tggaaaaatt caccgggttt tacttataaa atccttcgtt ccatcgactt
      781 accctcaaaa tttattgaaa taggcgaaac taatcaggga caatactttg attttttagg
      841 tgtatctgaa aaaccttcca ccgtttatta tcgcattgta cccaaagggc tgccccaaaa
      901 agaggcaaag aatataacct cccaaatagc cgttgaacga aaaacaatgg gtgacgagga
      961 cttgctcgac atgacgcagc gctacaccac ccgttatttt ttcgattttg ccgaaccgac
     1021 tacgggcatg gcacgcgaac ggagtaacga cgtgaatgga gacattgtaa ccaccggcgg
     1081 aaccggattt ggcatcatga gcctcatcgc cggtattgaa cgaaaataca ttaccccaaa
     1141 gcaggggcag gaaacaattg aaaaaatagt cagttttctg gaaaaaaccg aacgctttca
     1201 cggtgcctgg gcacactggt acgatgccga tacacagaaa gcattcagtt tcagcaaata
     1261 cgatgatgga ggtgatttgg tggaaaccgc ctttctggtt cagggattgc tgaccgcccg
     1321 cgaatacttc aaaaagggca gtgccaccga acgaattcta gccaaccgca ttactcaact
     1381 ttgggaaaca gtggagtggg attggtacac gcagggaacc gattcgctgt attggcactg
     1441 gtccaaaaac tacggatgga aaatgaacca ccgcatcaag ggatatgatg aaacgctgat
     1501 aacatacgta ctggctgcct catcgccaac ttaccccata aaagcatctg tttacgaaag
     1561 ctgctataaa aattccagct attatttcaa cgggaaaagc tattacggaa ttaaattaga
     1621 cctcggaatg gaatatggcg gaccattgtt ttttactcat tattctttcc ttggtttgaa
     1681 ccccaccgga ttaagagaca actacaccaa ttatttcgac agaaacaagg ctcacgcact
     1741 cattcaccgg gcgtatgcca ttgataatcc aaaaaaacaa accggttacg gacccgattt
     1801 atggggattt acatcttccg acgatccaaa agtgggttac acatcgcacc accccggtac
     1861 tgatgatgaa aacggtacga tatcacctac tgctgctctt tcatccattg tttacacccc
     1921 cgaagaatcg atgcaggtct taaaatatct ctatttccaa aaaggaaaac agttatttgg
     1981 aaaatatggc ttttatgacg cctataaccc cggcatggtg gaaggacaac aggtggttcg
     2041 cagttacctg gccattgatc aggggcccat tgctgtgatg atagaaaatt acagaagtgc
     2101 attgctctgg aatttattca tgaataatcc ggagatacaa aaaggattaa atttattagg
     2161 atttagatat accaaaaatt aacttcaaat tatcaaacgt atgaaaaaca aaagttttat
     2221 tttattactg gttggactga ttggtataag tacgttgttt gctcaacagc aacaggtcaa
     2281 aggaactgtt gttgatgcag ctacaggcga acagctgatc ggtgtaaaaa tccttgaaaa
     2341 aggtaccaac aacggtacaa ttacaaattt caacggagaa ttcacctttg ccgttccgac
     2401 aggcgcgcct attgtagttt catacatcgg ctacctcacc caggaactta aagccacagg
     2461 tacttcgttg caaatcaaac ttcaaccgga caccaagtta ctggacgaag tggtggtggt
     2521 aggttatggt acagctgcca aacgagacct gaccggttca atagtcaagg tttctgccaa
     2581 agaactatcg ggaaggccca atgccaatcc ggtttcgtcc ctgcagggga aagtgaacgg
     2641 attaagtgtg gttaacagcg gacaacccgg cagcgaaccc gatatccgta tccggggtac
     2701 tgtcagcaaa aatcagacaa aaccgcttta tattgttgat ggtattttta atgacaacat
     2761 caattttgta aacccggctg atattgagtc catggaagtg ctcaaagacc catcatcact
     2821 ggctattttc ggtgtgagag gtgccaacgg tgtgatcatt gttaccacca aaaaaggtgc
     2881 tgaaggcaaa gtaactgttg ggattagctc ttcgctcggt atgaagaata ttgtaggaac
     2941 accgcagatg accgaccgga cgggatttat gaccttgtac gacgaacaaa gagctaatca
     3001 gggatcggtt ccttatcccg attacggatt gttcagcggc aattccaact ggattgatct
     3061 tatttcaaat aataacgcat tggtcaatta caataacatc agtattacca gtggtacgga
     3121 caagaacaaa ttatacgttg gctttggcta cctgaatgaa gaaggactta ttaaaaatga
     3181 gaaatacaat aagtttacct tcacggtgaa tgatgaactc agtctgacca agcacctcaa
     3241 actgggagtg aatgtgaatg gctccaaagc caacctgccc cagttgcacg attttgtctc
     3301 ggccatcaac gcaaccccta ttgtggaagc attcaatacc gaaaacaatg tgtataacaa
     3361 acttcccgat cagattggcg gtccgcaaat cggtaacccg ctcctgtttg tggaaggtaa
     3421 taaattcaca tcgctggcca acgaaaaccg gtttgtaggc aatacattcc tcgaactgaa
     3481 tttcctgaaa aagtttatct tcaaggttag ttatttcgcc gatctgggct tcaacgacgg
     3541 cagatcctat actccattgt acaaagtgta taatgccgag tccgatcagg tggttaattt
     3601 taattccaaa tcagcggtta gtcaataccg gaatacgtac accaaatatc agcaagacta
     3661 tttgctgacc tataaagatc agttcggtga tcatggattg acgctcctgg gcggttttac
     3721 tacctatttc gaagacttta accaaataag cggtgccatt tcgcaatatg caggtggtga
     3781 cccaattcca aatgataaac gctggtggta tctgaacgta tatccgtatg gcgacccaac
     3841 cacagccact aacagttcgg ctgaatggga gcgttcaacg gcatcttatc tcttcagagc
     3901 attgtacaat ttcaaaggaa aatacaatct gaatgcctct ttccggcgcg acggttcgtc
     3961 cgaaatatca cccagccacc gctatcagaa tttctgggcg ctgggtggag cctggtatgt
     4021 aaccgaagaa agctttatgc agaatcagaa tatttttgac aacctgaaaa tcaaagcatc
     4081 gctggggcag ttgggtaatc agtataccgg tattcactac ccgtattatc cgaattatac
     4141 caccggttca tccaccgtat ttggcgaaaa agtagtgccg gcatatcagc ttgcctataa
     4201 aaacaatccg aacctgaaat gggaaaccgt ggacgcttac gaagccggag tggaaatgga
     4261 tctgctgaaa agtaagctcc atttcgaagc aaattactac gataagagaa ccaaagacct
     4321 gctcaccatg gtaaatgacg gagccaataa tttctacatg aatgccggat ctatcaagag
     4381 ttcgggattt gaatttctgg gaagttggaa ccagtctctt gccaaggatt tcggatatac
     4441 agtgactgcc aacctcacta caatgaacaa taaagtgttg agtgtgtgga aagaaggcta
     4501 caaatattat gatggaaatt caatcaccga agccggatat cccatcggtt atttctttgg
     4561 atatcaggtg gatggagtgt atcagtcgta tgccgataaa ctggcaagtc ccgtaaatac
     4621 gttgggcgat tatggacccg gcgatttgaa attcagggat gtttcaaagg atggtaaaat
     4681 caccaccgat gaccgtacga tgctgggcaa tcccacaccc gattttacct atggaatttc
     4741 agcaagtttg aactataaag catttgattt ctcggtagat tttcagggag tatatggcaa
     4801 tgaggtatgg cgtaactggg gcaatggatc cacctttgcc gtatttaatt tccggcaggc
     4861 acgccttgac cggtggaccg gtgctgaaac ctccaactgg gaaccaaaag tgggcaatga
     4921 agccatcaat aacctgccat cgagctatat gattgaagac gggagctatt tcagacttcg
     4981 taatatgcag ttgggttata cgtttgacaa aaagctgata tcgaaattac atatttcatc
     5041 cttacggctt tacgtgagcg gtcagaacct gttcacctgg aaacataatt ccggattcac
     5101 ccccgaagcc ggaggtagcg ccactcagtt cggtgtggat aatggaggat acccgatacc
     5161 ggctgtcacc acagcaggtt tcaatattaa tttttaacaa taaaaacaat acattattat
     5221 gaaacaagca tttaagcaag ctaaaatgag cgaacaattt cagggaatat aaaactgaag
     5281 ttagtcttca atcaacagaa aaagcagtgt ttctatgcta aaaaataaaa aattaaacgt
     5341 atgaaaaaca ataaattatt catagtcttg ttgccgttcc tattgttaat ggtagcaggt
     5401 tgctccgact ttctggatcg aaaacccctc ggtagtgcaa ctgaaggtga tttgaccgtc
     5461 ggtggagtgg aaggcaaagt atttgggttg tatggtaacc tccgtgacga tggtatgaca
     5521 ggtttaccca tggtgtttat gcacgtgatg cgaagcgacg atgctatcaa aggcagtacc
     5581 gttaccgatg gtgccggtta tgaacagatg gccgactatt tcaagtatga taaagccgat
     5641 ggctggctca cactgggata ctggaatgat cattacaaat taatttatgc ctgtaatgat
     5701 attttgcatg atttggattc gctcaaactc aactccgatg gtgacatgat caatcaggcg
     5761 gaagcaaagt tcttcagagc ctatgcctat tttgatctgg tcagaacttt tggcgaggta
     5821 ccaaaaattg atttcaaaat atacaacatt ggtgatgcaa acatccaaaa atcaaccgta
     5881 cctcagattt atgccctgat tgaccaggat ctcgattttg ccgcatcaca tttgccttca
     5941 ggctgggtga gcacctatac cggacgtgtt accagcggta cagccaatac cctgtgggca
     6001 aaaacaaaac tgtatcgaaa agactgggcc ggagcacttg caaaatgcga actggtaatc
     6061 aattcgggca aatacagtct gttggattcc tacgaaaagt ttttcaccga aaagggtgaa
     6121 aattcaagtg agtcaatact ggaagttcag caatatgtaa gtgctaacgg ctcggtaagt
     6181 tacagcaaca attgtaacgg cgcacaaggc gtacgtggca gcggcgactg ggatttgggc
     6241 tggggatgga atgtacccag caaagcactg gttgactcct acgaaagcgg tgatccccga
     6301 aaaaccacaa caattctcga atcaggcggc cccgacggtt acgggcttac tgttcctgca
     6361 tcattagcaa ccattcaacc ctattggaat cgtaaagtat attccgatcc ggcaagacga
     6421 tcttcgtcag gcattaaata tgccaactgg ctaaatatcc gtttgctccg ctatgccgac
     6481 gtactgctta tggcggctga agcagccaac gaactgggtg ggactcccaa tactaccaaa
     6541 gcactggcgt atgtggaaat gatccgtgca cgtgccagag gtaccgctaa tgtgttgcca
     6601 aaagtgactg ttaccgatca ggctgcactg cgtaccgcca tcaagaaaga aagaagagcc
     6661 gaatttgcca tggaatatga acgatttttc gatctcgttc gctggggcga tgcacctgaa
     6721 atattgggag ctgctggtta cacagccaaa tgtcagtact atccaatacc ccaaagcata
     6781 attgataagg cacagggcaa actgacccaa aacccgaact ggtaaaattc ataacaatta
     6841 atatactatc aatatgaaaa agatattaaa cagacttata gcattcacgg cagccatttt
     6901 gttgttaagc ggctgtcagg acatgaatcg cccggtgctc ggcgattatc ccaaagatac
     6961 caatcccgta ggaggacccc tgaaattcta cgtagcattt gacggaacaa ctaccgaccc
     7021 gctgatgaat gccgtggata gcatcaaagc taattttgcc acagccaaca ccaccacttc
     7081 catcgatggt gtacgtggaa aagccatgca gggtgctgcc ggaaaatttg tcagctacgc
     7141 aaaaccgaat gattttgtat caaaagcggg cagttttacc atttcattct gggaaaaaca
     7201 cgatggacaa acaaaaaaca atgccatggg caatggaccg gagtatccgt tcagttttgt
     7261 ttcaccatca gcataccatt ggtcaagtag caatatgttt cttctgtttg aaggaaataa
     7321 caccgcttgt gccgtgaaat tcgtagtggt ttcgggagta agtcccgatg gaaattccag
     7381 tacggccgat acatggctca cctgggaagg cggtgcaagc attcccggtt tactggacaa
     7441 cacctggcat cattgtgctt tcgtttatga tgctactacg tccggtctta cattctacaa
     7501 ggatggggta gctattggca caaaaacctg ggcaggacac ggacctatcg gactggtcga
     7561 ctccaaggtt aattccgttc gaataggagc cggacctcag ggaaatgccg gagatacatc
     7621 cgacgactgg ttgcgctcat cctggaaagg cggactggat cagttccgta tgtacggcac
     7681 agctttgaca gctgccgaag tagctgcatt aaattccggg aaaaagtaaa caacgattat
     7741 aaaagggttg taacaggcaa cccttttgtt tttttcaaaa acaattatta atcaaaaata
     7801 gaataacatg aaattgaaag tattgatatt agccttgtgt tgtctgatta taccttcttt
     7861 accggctcag aaaaccacaa aaaaaactgc aaagaagata gtgaccgaac aggttgagcc
     7921 ggttttgaat cccgataaat cgctgtattt cacgaatgat ccaaaaatgg ataaattcat
     7981 tgatgaattg ttggcgaaaa tgacactcga agaaaaaata ggacaattaa acctgccgtc
     8041 ggccggcgat tttactaccg gtcaggttca gagttctgat attgggaaaa aagtgctgga
     8101 aggaaaagta ggtggactgt ttaatattaa aggagtagat aaaattcggg atatgcagcg
     8161 aaccgccgta gaaggaagcc ggatgaaaat cccactgatt ttcggcatgg atgttattca
     8221 tggttacgaa accacattcc ctattccgct gggacttgct tcatcgtggg atatggatct
     8281 gatcagaaaa tcggcgcaga ttgcagctgc cgaagccacc gccgatggca tcaactgggc
     8341 tttttcaccc atggtggatg tttcccgtga cccgcgctgg ggaagggtag ccgaaggagc
     8401 tggtgaagat gcctacctcg gtagccggat tgccaaagcg atggtgaacg gatatcaggg
     8461 gaatgacctc aaagccaaca atacgatgct tgcctgcgtg aagcattttg cgctgtacgg
     8521 agcttccgaa gccggccgcg attacaatac ggtggatatg agcaaggtaa gaatgtataa
     8581 cgaatatttg cctccttaca aagctgcaat tgatgcggga gccggtagtg tgatggtttc
     8641 gttcaatgaa attgatggaa ttccggctac gggaaataaa tggctgttga ccgatttact
     8701 ccgtaaccag tggaaatttc ccggatttgt tgtgaccgat tataccggta ttctcgaaat
     8761 gatagaacac ggaatgggtg atttgcagca ggtatccaaa ctggcgttga atgccggtgt
     8821 ggatatggat atggtgaccg aaggatttct tctcacactg acaaaatcat tgaaagaagg
     8881 aaaggtaacc tattcagata tcgatttggc tgcccgccgt attctggaag ctaaataccg
     8941 gctcggatta tttgacgatc cttacaaata ctgcgactcc aaccgggcaa aaacagagat
     9001 ttttaactct caaaacagag cggaagcccg cactatagct gcacaatcga tggttttgat
     9061 gaaaaaccag aataacacct taccgctcaa agcagccgga accgtggctg tgatcggtcc
     9121 actggctgat aatcctgaaa atatgccggg aacctggagc gtaggtgcaa aacattcaca
     9181 ggccattagt ctgatgaaag gactaaaaga aacactgggc gataaggtga attttgtatg
     9241 ggctaaagga gctaactttg attatgatcc tgattttgag agccgtgtat ccatgtttgg
     9301 caaaaactca tatcgggata atcgtccgaa ggaagaaata ctgaaagaag ccatggatgc
     9361 agcccgcaag gccgatgtca tcctggctgc cttgggtgaa tcggctgaaa tgagcggcga
     9421 atgcagttcc agaaccaata tcgaagtacc tcagtcgcag aaagatttgc tcatcgaatt
     9481 gaaaaaactc ggaaaaccta ttgtactcgt tctttttacc ggtcgaccat tagtgcttaa
     9541 tgacgaaaat aaacttgccg atgccattct caatgtgtgg tttgcaggaa gtgaagccgg
     9601 atatgccata tctgatgtcc tgtttggaaa agtgaatcct tcagggaaat tgccgatgac
     9661 attcccccga aatctgggtc aggtaccgat ttattacaac cacaaaaata ccggccgacc
     9721 ggaaataaag gaagaccagt ttgataaatt taaatcggtg tatctggatg ttcccaattc
     9781 tccgctgttc ccattcggtt acggactgag ttataccaca ttcagttatg gagatatcag
     9841 tctgagcaat acgcagttgc agggaaatca aaccctaaaa gcaagcgtta ccctgaccaa
     9901 taccggaaaa tacgatgggg cagaggtagt tcagctttat atccgcgatg tggtgggaag
     9961 taacacccgt ccggtaaagg aattgaaagg atttcagaaa gtgatgctca aagccggcga
    10021 aacaaaaacc gtcacgttcg atatcactcc cgaattgttg aaattttaca acaacaaatt
    10081 gatttacgat tgggaaagcg gtgactttga aattaaaatc ggaggaaact cctacgatgt
    10141 gaaaacagct acggttaact ggatgaaata attaatcaca aacctttccc ggataattta
    10201 ttttgggaaa ggtttgtttt attatgaatt gtgatgaata agattgttgt cataggattt
    10261 ttatgcgtgt tggcaatgtt ctcctgcaaa aaggaagaac ctaaaccacc ggttgtcaaa
    10321 acccgtgtgc tccggataat cagtgtgcag atcaacgacc agacctgggc cgataattct
    10381 gtgatttaca atgtgagtgt ggtcggtaaa ccggtcatta aaatcacatt taatgatacc
    10441 attgatgtga gcaagtttgt tgcgtccaaa gcaagtttca gcggcgatat cgggcagaat
    10501 tttaccgttt ccaaaggtac tgatacgaaa accctgctta tcatgcttac cgttttaccc
    10561 cgtgatctga cagcctatac tttcaatctc gactccggcg aaaacctcgg aggaagcatc
    10621 tatgcgtcgt tctcatctgt ttttgtcaca caactggata ccacacccaa attcccgact
    10681 atttccaccg atagtctgct cacgctcgtt cagaaacaaa cattccgtta tttttgggac
    10741 tatgcgcatc ccgtttcggg tcttgcccgc gaacggctgg gttctggcga aacggtaact
    10801 acaggtggct ccggatttgg gctgatggct attctcgtgg gtattaaccg tggtttcatt
    10861 acccgaaatc agggatttga gcagataaat aaaattgtta actttctgaa caaacccgaa
    10921 accgataaat ttcacggtgc gtttccgcac tggatgaacg gaacgaccgg aaaagtaatc
    10981 cccttcagca ccaaggataa cggagccgat ttgatcgaaa catccttcct gatgcagggc
    11041 ttattggctg ttcaggcctt tttcaaatca ggaaattcgt cggagcaagc gatgtgtgcc
    11101 attatacaac agctatggga aaaggtggaa tggtcgtggt ttcagaaaaa cggagaacaa
    11161 aaactctact ggcactggtc gcccaactat cagtgggata tgaatatgcc catcacgggc
    11221 tggaacgaag gattgataac ttatgttctg gctgcatcat cacccacgtt tcccattact
    11281 aaatcggtgt atgacaacgg ctgggcaagt aacggtgcta tgaaaaacgg aaattctttt
    11341 tatggtgtta ccttacccct gggtgaagag ttaggcggac ctttgttttt tgctcattat
    11401 tccttcctgg gacttgatcc gcgctattta tccgatacgt atgccaatta ctttacacaa
    11461 aatcaggcac atgcacgaat caattacaac tactgtgtag caaatccgaa aggatacacc
    11521 ggctacagcg aaaattgctg gggccttact gccagtgact ttcccgatgg atatacggct
    11581 tcatcgccca ataacgatca gggaacgata gctccaacgg ctgcgcttgc ttcttttcct
    11641 tacacgccgg ccgaaagcac aaaagcgttg aatttctttt attacacgct gggcaatcgt
    11701 atgtggggcg aatatggatt taaagatgcc ttttcgctaa cacggcgttg gtttgccgac
    11761 tcatatctgg ccattgatca gggacccata atcgttatga ttgagaatta tcgcagcggt
    11821 ttgatctgga acaccttcat gaaccatccc gatgtgaaat caggattgac aaaacttggg
    11881 ttcatttatc aataaaacca tcagaaattg aaaaaaattt acaaaatgtt ttgtttttaa
    11941 agataattgt atagttttgc ataaatagtg tttaattaaa aattaaaatt tatgggtttt
    12001 gtaaaagaat ttaaagaatt tgcaatcaaa ggtaatgtaa ttgacctggc tgttgcggta
    12061 attattggtg gtgcattcgg taaaatcgta gcatcatttg tagctgatat cgtcatgcca
    12121 ttaattggcc tcttactcgg cggtgtaaat ttcaccgatc tcaaatgggt gattaaacct
    12181 gcagaaattg taaacggagt ggaaacagtt gctgccgcta cattgaacta cggtaatttc
    12241 attcaggtta ctatcgactt cctgataatt gccctggcta tttttatagc tatcaaaggc
    12301 atgaattcat tgaagaaaaa agaagaacca gctcctgaag cacctgcagc tccatccaat
    12361 gaagaagtac tgcttggtga aatccgcgat ttattgaaag caaaaaaata aattgctgaa
    12421 aacctactac tgaattcgtt ttgataaggt ttgaattatc ctatcaaaac gaattctttt
    12481 tgttactttt gcttcatgaa atatcgcatt caggtttcaa agcaaatgcg gggagacaaa
    12541 tcttttttcc ggaaaataat tttaatccct cattgtctgg cattatattt gactaaccgt
    12601 tgatgatagt tttttttatt aattctttca atttttacaa atcatgaata agcttattgt
    12661 tttgttgggt gtattactca cagttctcac cggatgtacc gaagccaaaa agttaacaca
    12721 gtttgacttg ccggtaaacc aggaatttac gctaccggca ttaccaattg ttgcgacaaa
    12781 tactgaattt accacaccag agtttgaaac caattacctg agttatttcg aaacattcaa
    12841 tatcaatacc gatttggtgg aaagtatcgg aataaaaagt gtagatttta atatcacagc
    12901 accggaaaca ggcgatttca gttttctgca atcagtagaa gtctatatat cggccgaggg
    12961 gttgaatcag gtattactcg catcgaaaaa aaatattaca aacgccggga aaaccctgtc
    13021 actggatgta gacagcaagg ttgatcttaa aaaatatgtg acaaaaaaca atatcaaact
    13081 aaatttgata gtgagcacga ccaaaactac cacagcaaaa tatactatag acactaagct
    13141 cgtttttttg gtggatgcca atgtaatggg gctttaacat acgatatttg cctgtaactg
    13201 aagccggctt tgaatttttt caaagccgtt ttctgtttga cctgaaaaat ttaattaatt
    13261 ttacaacctg aaattgacaa tcaatccttg cccatcaatc cagaccctga tgaaaaaaac
    13321 tgccggattt ttcttttgta ttttcttctg tctgcctctt tcagctcaga tacacgccgc
    13381 agattcaact gtgcaggtgg ctggtcactg gcatatgcac gacaaacaaa actatcacgt
    13441 aactgaagaa tccaacgtga ttaagaatga agccgacacg attaatgccg caaagataac
    13501 atatcagctt gatattgaaa tacttgatac tagtgaagat tcgtatacca tacagtggct
    13561 ttcacacgat taccggataa ttaaggcaac cgaccggcaa ctgataccac tttataaact
    13621 gggagaaaat tcaagaatag tattccgtac tactgagacc gggaaattta aggaaattgt
    13681 caacggtgat gaactcctgg aacacaacaa aaaggaattt gaactcctcg tatcccaata
    13741 cgatactatt cccgctgtga aacaatggat agataagttt gaagatcggt atactacccg
    13801 ggaaacaatc gaaactactg ctgccagact gattaatcag ttttatgtgt ttcacggagc
    13861 caactataag cttggcgagg aagttaattc gcaggtaaag ttatccaatc cgtacagcgg
    13921 catacctttc gatgctgtaa tgactgtttc gctcgatgag atggacgcgc tcaacgatta
    13981 ttcaatcctg agaatgtggc agacagccaa ttcggcacaa ctgagccagt ttgcccaaaa
    14041 cctgataaag gaagttgccc gggaaatgaa tgtggacgta aaggaaagtc ttcaaaacat
    14101 ctcacccatg atcaacgaaa cccgcattgc ctcgcaaata cacggttcca caggctggat
    14161 aatttacagt acgcagacta cagaagtctc gctggataat gtactgaatc tggaggaaat
    14221 cacgattgaa cttcaataaa ataacaagaa agaaatatga caaagtatcg gtttatcatc
    14281 agtctgattt gttttgggat ttttatcatc ggatattcac aaaaattcga tgcccgattg
    14341 gccaaaaaat taggtgccga tgaatatggc atgaagagtt atgtgctcgt cattcttaaa
    14401 accggcacca acaccacaac cgacaaacaa acccttgatt cacttttccg cggtcacatg
    14461 gacaatattc agcgactgtc ggctcaggga aaactaataa ttgcgggacc catgaaaaaa
    14521 aatccgaata attacagggg aatttttatt ctggatgtaa aaactcagga tgaagcacac
    14581 caattgctca gcaccgaccc taccatccgc gaaaagatct ttgatgtgga actttacgat
    14641 tggtacggat ccgcagcatt gcccacctat ctgcctgcac ataccaaaat cgaaaagaaa
    14701 agtattaata ataatacact taatttaaag taattagttg tcatgcatca aaaattattc
    14761 tttttaatca cccttttcac aggagttatt ttctcatcct gcaaaacgat acaacccgaa
    14821 gctccgaagg agtcctattt gcctaccgaa attactgtag cctattccga acttccgatt
    14881 catatggagc tggacgttaa gaagcttgaa aaagccgtta ataccagcat gaacggattg
    14941 ctgtacgaag gttcaaacat caccgacaaa gatttatcgg taaaaatatg gaaaactgca
    15001 gattttacat tcaacattaa taataatgcc atcgaatacc gggtgccact caaagtttgg
    15061 tcacaatttg cctggaaagt ggaaaaactc ggattgtcac tggccgatga ttatgaagca
    15121 accggtacta ttgcgctgaa atacaaaact gtcatcagca ttgaccgaaa ctggaacctg
    15181 gtggcgcaga ccacatcgca gggctacgaa tggatagaaa ctcccaaact aaatattatc
    15241 ggagtgaatg tgccggtaaa acctattgct aatctggcaa tggcgaaata tgagaaaatg
    15301 attactcagc agattgataa atcactggct gaattcgccg atctgaaaaa acaggtcacc
    15361 gatgcctggg cagagttgca aaaacccgtt cagacaagcg tggaaaatga tatctgggtg
    15421 cgtattattc ctaaagatat actggtttcg ccttttgcaa caaaagccgg aaaactcaat
    15481 atggatattg ccttgtatgg aaaagtggaa acaataatgg gcgcaaagcc ggctgccggc
    15541 aagccggtgg cattacctga atttaagtgg attaaccggc caccgcagca gtttaatctg
    15601 aacgtgagta ccgatgtcac tttcgacaag atttcgcaat tggctaaaga gcagttgctc
    15661 aataaaacct tcacagaagg gaaaaagcac attcagataa ccgatttatc cgtttttggc
    15721 agcgagggga aggctgtttt ttcagctgac gtgattggtt cgatcaaggg gcggatttat
    15781 tttaccggag agatgcaata taatccggca aaaatgaccg tggaaattat caatcccgga
    15841 tttgaattaa aaacgcgaaa tgccctgctc aaatcggctg attggttgct tcacggattg
    15901 attctgaaga aaattgtgcc ttatctttcg tacccggtca aagaatacac cgatgatatg
    15961 aaagcagaag ccaacaccat gcttcaaaac tatcaggtga gggaaggaat aaatcttcag
    16021 ggaaaactca atgaactgac tgtgaaaagt gtgaatcttg tgcccggtgc cattcgtatt
    16081 caggcaaact tgaaaggcaa tgtgatgatt aaacttgaaa atatgaaatt ctgagataca
    16141 tcaggggtta ttttttcttt tgcccccttg tttttggttt cttgtatttt tgtttcaaag
    16201 cgtccttacg gcggactttc atattcacct ttttattctt gtcttttttc tcatgaaaag
    16261 ccggtccgtg tatttctctc tttgcaatga aagccaccga gtttccattc tgaatctgtg
    16321 gtatttcaca gtcggccagc tccgttgaaa cgactacatc ttccggcatt cgcatcatcg
    16381 gaattttttt ccccatcagc tgctgaatgc tctcacggta ttctgtatca tctttggtga
    16441 taaagcttac tgccacccct tttttatcca cacgtcccgt tcgcccgatg cggtgcatgt
    16501 agttttcggg ctcgtccgga atatcgaaat tcaccacgtg ggtaatgtca tcgaaatcca
    16561 acccacgagc cacaatatcg gttgcaatga gcactcttaa tttgttgtca ttgaatgatt
    16621 tgagggcttc aaagcggttg ttctgtgatt tgttggagtg gataacacct acggtttcgg
    16681 gaaaaatcgc cgaaatccgg ttgtaaacat catcggccag ttttttggta gaaacaaaca
    16741 acagcacttt agaaaatccc ggttcggttt tcaacaggaa ttcaagcaga ttgattttgg
    16801 tgttgaagtt gggaacttta atacccgttt gtttgatatt ttcgagcgga gtacctgtgc
    16861 gggcagcttc tatgtgtagc ggatcaataa agtaatcggc aataaaattc tgaatttccg
    16921 gggtcatggt agccgaaaac atcaaatttt gccgtttttt gggcaataaa tccatcacgc
    16981 gtatcagttg cggacgaaac ccgagattta gcatttcatc cacttcgtcg atgataaaac
    17041 gcttcactaa tttcagtttc agggtgccgt tgagtgcaaa atcgagtaag cgccccggag
    17101 ttgccacaag cacatccagc ccatcaaaaa taagcggtgc ctgtcggatc atattctcac
    17161 ctccatacac acctgccacc cttattgtca tgtaagtggt cagtttttgt acctgttcaa
    17221 ccacctgaac caccagttcg cgggtgggaa caacaatcag tatttgagga tgtttctcct
    17281 gtgtgaattt ccactgacgc aggcagggaa gcaggtaggc aatggtcttt cctgtaccgg
    17341 tctgagctat acccaccata tcgcgtcccg acatgatgac cgggtaagct ttctcctgaa
    17401 tagttgttgg tcgtgttatg tccaggtcat tgagtgcatt aataagcgga gtattcaggt
    17461 tgagttcgga aaattgcatg ggatggatat tttttggcaa gtgtgatgta atcgaaatag
    17521 atgtttttaa atcgggtgca aagatacgct taaaatatag aaaatcaact atataataaa
    17581 ttcaaaaacg aaatggaagg tgaaaactga catttttcga aataaaaata cggatatttt
    17641 tgtgacaaaa aaaattaatc ttcatccgtt tcagcttctt cttctgattc cggtaaatct
    17701 tttttgcaca ccagaatact cacttcgtag agcaaataaa tgggcagtgc caccaccagc
    17761 atggtaaacg gatcggtcgt gggagtgatg actgccgcta caataagaat ggttacaaaa
    17821 gcatatttcc gaaacgtttt cagggctttg cgcgatacca gtccgatgcg gttgagtgcc
    17881 aaaatgataa tcggcagttc aaacatgata cccaggctga aaataagaat agccagcgca
    17941 ttcatgtacg actgaatgga aatctggttg gtgaccaaat cggatacctg ataggttccc
    18001 aaaaaacgga tggtaagcgg aaaaataaga aaatagctca ccagggctcc cagataaaac
    18061 aggaaagaag aagtgataaa aatgagactt acattcttac gctcattggc atacaaggcc
    18121 ggattgataa acctccatat ctcaaatagc aaataaggca tcgataccac caccgccatg
    18181 atgagtgagg aggtgatgtg ggtgacaaac tgacccgaaa gattgatatt gatgagctga
    18241 accctgaaat catcgggaca caagcccggc attttcagtt tttcgcccag caggcaaaat
    18301 aaccgatagg tgtaaaaatc ggaattgagc ggaggcagaa tgatgttgga gaaaacataa
    18361 tccttgaaac caaaaaacac gaacaacaat agcgtaacaa ctcccaggct tctgagaatt
    18421 gtccacctta acgcttcgag atgctcccag aaggtgaaat cttgttcgga atcaggtttt
    18481 cccaatgtgt ggttattgaa aacgaataaa aagtctgtag tgtcaaatac gggtttattt
    18541 gtcggtttcg tctttcagat cgtttttgat ttcatcgtcc acgtctttca gccctttttt
    18601 gaaactgttc acccctttgc ccaaacctct catcagttcc ggtatttttt ttccaccgaa
    18661 aatcagcaat acaaccaacg cgataagtat aacttcggga gcgcctaatg aaaaaagcag
    18721 aatatttcca gtcatatttt taaaaattaa ttttttacgg aattgttgat gcaaacttac
    18781 gtaaaaaatt aaaataactg tctttgatta cataaaaaag caaactgttg aatgcaattt
    18841 gcttctgaaa taaatgattt cgtcaaaaaa aaggaatatt aacccgcatt tttataaatg
    18901 cttttactga agagtgtgga cattattatt ctcaaatccc agataaacgt ccatccttcg
    18961 aggtattcca tatcgaactg cactcttttt tccattttcc agagttcatc tgtcagaccg
    19021 cggtagccat tcacctgagc ccagccggta atgccgggtt tcacaaagtg gcgcactttg
    19081 tagtgttcta tcagggcaga atactgcttg gtgtgtttga gcatgtgcgg gcgcgggcct
    19141 actaccgaca tgtcgccctt caacacattg aggaattgtg gcagttcgtc gatattcgtt
    19201 ttacggagca atttacccac cttagtaatc cggtcatcat ccttagtagc ctgtcttgtg
    19261 tcagcatcca gattggttcg catggtgcga aacttgaggc aatagaatgt tttgttgttg
    19321 ataccggtac gccgttgaat gaaaaaaaca ggcccttttg attcaagctt gataatcaag
    19381 gcaataagcg gaaatagcca gctgaaaagc agaatgacca ccaacagcga aaatccaatg
    19441 tcaaacaatc gcttaccggc acgataagtc agtcggtcta aaggtatttc ctgggggttg
    19501 aacatcataa atgaaccaac cgcttcggta tttttcaccc tggctttgaa ccatgcctga
    19561 tttacaggca catatctaac ccttactcct atcttgttgc ataaggcgag caattcccgg
    19621 gtgagattgg tgtcgtagta ttttgggagt gtgacaaaaa tcatgtcaat atttaattca
    19681 tcagtcaacc gctccaaatc atcatacgac cccagaattt ttttatcaca tttttttatg
    19741 tcatgatttt cagaaataaa accaaccaga ttgagcccca gttcgggatt gttttttatc
    19801 agatcaccca gtatgacacc cgttctgttc aggccaagaa tcaacactcg atgaacggat
    19861 aacccttttt tacgccggta cttgtagatt tcgtaaatca aataaaaagc gacaagcttt
    19921 atcgcataaa ataatgccgt gtatttcaat agaaataagt acggggtagg cttgtgtaaa
    19981 aatatctggg ctaaaatcag cagggcaata acgaaaataa tcattctata actcagatac
    20041 ttcacccggt tgacaaatct gtcgcgaaaa taatagtttc ttttggagta caacgaatag
    20101 gcaatcaatt ccgaaatatt accgtgcaga taaagtaaac cgatatcgtg caaattcatt
    20161 tgggtaaaaa caggactcag tgaaaaaacg atagctatgg caacgttaag cagtaccacg
    20221 tccaggatag catacatgaa tttcaggcct acttcgtcgg aatccataat tgtaaatctt
    20281 aaaatgtcag ataaataata tattaaggca aaaacgttag gttcgtttag ttattcggga
    20341 ataagtttta caacaatagc tctatttaag atccttttta tttatatcct gaacaggaac
    20401 ttcttcattt atcagtaact tgctatcgaa ttgtaactct tcctgcttga tattaccggt
    20461 tgaaaacagc attttttcac gcaatatgta tgctatgaat ttcacattgc caatcaggta
    20521 tcttcgccac atccgccgcg gttcttttat cagtcggtaa aaccattcca agccaatttt
    20581 tatcatccag gcaggggcgc gtttcactgt tccggcataa aaatcgaata cagctccgat
    20641 acaacacacg tgacccgctt tgaggtgatg gaaattactg tatgcccatt tctcctgctt
    20701 gggcgccgtc atccccacaa aaagtacatc cggctcatgc tcatttacag catcaatcat
    20761 tgtgtgggtg tcctcatcgc tgaattcgtg tttgaaaggg ggtgaataac tgcgcacctg
    20821 aatgttggga tattctgttg ctgctctgtt ctgtataagc cggagtgtgt tttcggaact
    20881 tcccaggaaa aaacatttac caccgctgcg gtttattcgc tccatttcat acaggaataa
    20941 atcctctccg gcaatttttt ttattttttt tccggtcaac atccgcatcg ccatcactat
    21001 actcacaccg tcgggaagta atacatcaca actttgcagc gctttgtgaa aatcctcatc
    21061 tttctgaaca gtattaaacg aatgcgcatt tagagcaccg ataagtgcct ttggttggct
    21121 gatctcgttt aatgactgat taaatattgt gtaatcctgt aacattgact taactttaat
    21181 attgtaaaaa aacaactaca tcaattacaa aatcatacac cttagaatga cctaaccgaa
    21241 actcttacat aaaaaaaatc aatcgttcag attacagttc taattgttgt atattaaaaa
    21301 cattccatat cctatatagt tttataattt tattaatttt ataaatttta atactgtagg
    21361 cagtgttacg ttagaggtaa aaaccattta ataaatttct tttcacaggc ccattttgtt
    21421 tgtttttttt accattgcaa tgaatcacga aaaatgtaat gaccgccgaa atcattataa
    21481 aaagacattg ttgaacaatc aatgatagta aacgtttaaa aatagacatt aataattcat
    21541 ccaaacctat tcatacatga ttgtttgtca catgatgact tttgggggtt aaagaatatg
    21601 ttgttattat ttgagttgct gaataggaaa gaattcttac tcttatccac ccggattgca
    21661 atgaacaaaa ttacatccca tcccattctt gatgttcctg atggcgggga aacttccttt
    21721 caatttgaag gaaaaacaat cgtatgcgat aaaggtatca ccgtggcagt agcgttgcac
    21781 cgcgcaggtt tttctgtaca cagtcacagc cttgacaatc gcagccgaag tcttcagtgt
    21841 ggcatcggta agtgtggtgc ctgcgaaatg ctggtggacg gagagatacg ccgcatttgt
    21901 atcaccggcg ttgacggagt acgtgaagtg cgcgaggtgc cacgcgatta tttgcctcag
    21961 acaaagcaac acgacccgac aaaatctgta agcgtctaca aaacgaccgt ggccattatc
    22021 ggtgcaggac ctgccgggct ggcttgtcgt gaagagttac gtaaacataa tattgagaat
    22081 atcgtattgg acaataacaa taaaatcggc ggacaatttg tgatgcaaac acattctttt
    22141 ttctttttcg aaaaagagaa aaaattcggt ggtatgcgcg ggttcgatat cgctaaaaaa
    22201 cttgcaggag ataatgagga aggcattctg ctgaatagta ccgtctggga tatactggat
    22261 ggaaaacgta ttgctgccaa aaatataagt actgaagaaa ttttttatgt ggatgcagac
    22321 tatcttgtcg ttgctaccgg agcagtaccg acaatgcctg tatttgaaaa tgatgacctg
    22381 ccgggagttt acaccgcttc agttatacag aaaatgatga ataccggttt caccttaccc
    22441 ggaaaaaatg tgcttactgt tggtgccgga aatgtcggtt atcttacatc gtaccagttg
    22501 atgcaggcgg gggctcgtgt caaagctatc attgaagctc aaccggttga ggggggattt
    22561 ccggttcagg ctgatcgggt acgacgtctc ggaattccca ttatcacctc acaccggctt
    22621 atcaaagcca tccccaatga tacactgacg ggtatcaccg gagctgtaat agctgaatgc
    22681 aaaaacatgg agattattcc cggaacagaa aaaataattg atgacattga tacaatcaat
    22741 atatgtacag gtcttacgcc tgataatctg ttgattacga aaggcaacga agtgttcggc
    22801 cggaaatgtt tcggtgccgg cgacgccatt cacattggcg aaggtactac cgctgtattg
    22861 tacggcaaaa aaacagccct cgacatacta caggaaatgg gtgtgaaaat caattacgaa
    22921 gaatacctca gtctgacaga agaatttata cagtctcagc atcatccagc gcgtgtcatc
    22981 cggcagcctt acctgcccac acccgggcga atgagtgaaa aacccttcgt gctgattgat
    23041 tgcctgcata gttttgcctg caatccgtgc gcatttgcct gcccccatga agcgatcacc
    23101 aaatcaacca ccaattcagt tcctcaaatc gatttcacga aatgcaccgg ctgtatggag
    23161 tgcgtgcatc aatgtccggg attggcgatt ttcggatatg cacccaaaaa taagctgatt
    23221 tatatccctt tagagttttt ggctgaagaa aatgaaaccg tatttctcgt taataataca
    23281 ggtgaaaaac tcggtgaagg cgtcattgtg aaaatattgc caaaaccgaa caaaaccaac
    23341 atcgctgttg tgaaagctct aaatctgaat gaagaagaat tattgcaggt caggggattt
    23401 attgctaaga aaaattatcc tgaaccgttg gagttgaaac cggttgaatt taaggaagaa
    23461 cagaaaatat acctttgcca ttgcgaggat gtaaccatcg aaaaggtatt gtcggctgtg
    23521 ggaaaccgga aatttatttc aatcggagaa atcaagcaca tcacccggtt gggagctggt
    23581 gtctgtcggg gtcagttgtg tacggaccga ttgaaatcgg tgatgaaaga gaagggaata
    23641 acccttaccg gagatgcggc tccccgtgca ccattgtcaa atcaactgtc actgggtgaa
    23701 ttgattacgc ccacaaaaaa agagaaaatt gttaccggaa aaaatatacg caaaatagaa
    23761 gtaccggtat tgattgcagg cggagggatg gccggaagtt cgctttatcg atattttgca
    23821 gaatcgggat acaaaccggc tttgataaat tcaggtcggg gttcttcctg gagaaatatt
    23881 gccgccggaa gggctgtgtt tggcattcct gaaattgccg atattgcccg gcataatctg
    23941 gagatattca aagaattgca acagatcagt gacatccact ttcgctctac gcgatttgtg
    24001 aattttatcc acaacgagac attgtacgaa atcatggaga aatcgcaatc atggtcgggt
    24061 gggcaaatca tagaatgcga ggattttacc aaaacaattt cgcctttcgt taatccggat
    24121 ttgaaaaaaa actacctggc agcattcatt acgcctgatt gctggcaggc cactccgggt
    24181 ctggtcatcg acctgatacg ctcgctggga agcagggcag gagggactat ttacgaagat
    24241 tgcgaactga tggaagtgca caaagagaat gataaattca ttgctttggt aaaagataac
    24301 gaaagcaact atattgaatt ttatacaccg cattttgtga atgcactcgg agcggatgcc
    24361 gaaaaatttg ctgcgcaact gggcctcgaa acgggcttgt atccggtgaa acaacaggca
    24421 tttatcaccc gccgcctgcc taatctggga attaataacg agccgctcga tatgctgata
    24481 gaccgaacaa aggtaaatgg atttaccaat ttttacgctc agcaacttct cgaaaccggt
    24541 cagataatcg gctgtgccga aacagcttcg gaaccggccg atgccaataa aaacatccgt
    24601 attaacacgc aggattttat ccggtcagtc tccgaaaaat tggttaactg gattccgcgg
    24661 ctttcatcgg ttggattgca ggccgtttgg gcaggttatt atgtagaacc ccgaatgata
    24721 gttgaccctg aaaatggctt attggccggt ttacagggct cgggttttat gttcagtcag
    24781 tacctggcca aaatgtatgt ggataaacta tctggcaaaa aagtgcccga ctacttcgac
    24841 cggctgaaac tcgatggtga cggttttaaa agaaaaaata tttgataata tacacgtatt
    24901 cagttagttg tgcattagta attttttaag aaaaatccac aattaagtca ctgcgaatca
    24961 gatgtcgggt gtccaggtaa ttgtatagcg caacgacgat gtacactgat gtgtttcctt
    25021 gaaacggatc agcccttcgt tgggtatgcc tgatttgctc gaaagcgcaa ggtcaatgta
    25081 gtcaaatccc agatttttgt aatgaataaa taaattgaat accagaaaat ccattatccc
    25141 aatggacgtt cggggtataa tatcacccca gaaaataccc tgtacaattt tcggatgtcc
    25201 gcgataaaaa attgctgctc cgatattatc gccctcttta ttcttcacca gaaaaaaatc
    25261 taccggaaac agcctgttca catccatcaa atcgtcaaaa ctcataaaga tctcccgtcc
    25321 gagttgagta cggttattta caatgacttc atacgattcc ttcatcgatt tttcgtcggt
    25381 gaccagactg aaggtaaggt tgttttttat tgccgattta caattttctg caattctctt
    25441 ccttttccag gtgccattaa atcggttcag atctatccag cttactaaat ccggaatttc
    25501 cagggtgtaa ccctgcctta cgaacgcatt tacaaattta gcgtttattg agctgttgta
    25561 aatgttgggc ggaagtatga atgacatttt attcaggtgc tgatccacag catattcctt
    25621 cagctgagcc agaaaattaa aaatttcgct gtaataaacg tcgtcgtggg tataatgaaa
    25681 tcctccaaac ggagcagaaa agggagaact gagttcgttg tccttcaagc cggcaataag
    25741 tccgagcgaa atatttcctt cattagtaat caaatgtacc actttttcgg ttttttgttt
    25801 caccagatca ataaaccctt ctgtgatgta aggatgagga tcttccgtaa aatgacttct
    25861 gtaagtctct ttagaagttt ctgtaagcat aataataaag ttttaggtta atacgttgta
    25921 aacaaattct gttcgattgt ctggtttaat atactgttca tttttttttc aaaaatatcc
    25981 agtgtaaatt ccttttcata tttatgcctt ccggcttttc ccattttcag acgcatgtct
    26041 tgatttttta ttaaaatttc cagtttttca gccagggctt ctgcatcgtt ttttggaatc
    26101 aaaaagccgg ttttatcatt atcaacaata tccgaaattc caccttcata agtagatata
    26161 atcggtaatt gatgttgcat tgcttccagt aaaaccagcg ggaaacaatc attcatcgta
    26221 ggatgtacaa atatgtcggt ttcggtaaat gcccgatgct tatcaattcc aaatttttgt
    26281 cccagatatt ttacattatc agttaattcg ttttcattca ggtaattgtt gaaattatca
    26341 acatcgatat ctccaagtcc gccaataaaa ctgcaatcga aatcaacacc attttgtttc
    26401 aacatggagc atgctcttaa taatacaaaa acaccttttg aatgcatcag attagacaaa
    26461 aaaagtattt ttaccgtttt ttgaccggaa tcggtgtggg tctcaacgtt tgtcagggtg
    26521 tcttttattc cgttaggaca acaaaatatc ctcgaggaag gtacgaatgt ttccacgtcg
    26581 cggtacaact gtcgggaaag tagaatgaca tgagaatccc gaaaaacaaa tcgatataaa
    26641 agataattta ttttttttgt ttcattttgt tttacgcctt gattatgaag atgataaatg
    26701 cactttatgc tgaatatgcg caataaagtg accagaaaaa tgtctttgta gaaagctgtt
    26761 ccctttactg taagcgctaa gtagcacaaa tccggttttt tcctgatgat ttcacccaaa
    26821 accctgaacc acagtcctat aaaccgaaaa actttcaatg cacccggttt cccggtttcg
    26881 ctgaccgtgc gcgacatcag caggttgaga tagcgacagc ggaaagtact gttgatgagc
    26941 tcactatctg ctattgcctt tccaaccatg gaagagccgt gtaccggcgg cggtacatgc
    27001 aacaaaaaca atatgttttt cttttcactc atttattctc ctgtatagtt cagcataggt
    27061 atcaatcatt ttattcatcg aaaaatgttc attaacaaac cgaaatgcct gatttttgag
    27121 cttttcgtaa tcgtaggatt tgttttttat cttttcaaaa accgccatca attcttccct
    27181 gttttctttg tgaaacatca gtggccagtc gtgcggcagc gtttctttca gtccggaggc
    27241 aaatgtggca attacaggaa ttttggcaaa agacgcctcg atggacatta atgcaagtcc
    27301 ttcgaaatgt gacggcataa aaaggtaatc aaagtcgtac agtttcgcgg caatatccgg
    27361 aacagcctca tacacaagca cagtatcgta atcatttgcc agtttatcta tctcgtgttg
    27421 aaatgtacca cttccgatga tatgaaacag aaaatcgccg ggataaagcg cgtaaatcat
    27481 ttgaatacac gggataagaa cgtccatacc cttataatct tcaaatctgc cgcaaaaggc
    27541 aatatttatt ttggcagtat caatattgaa aggatggggt tttcgatcgg gaataataca
    27601 tccgttatta ataatctgtt gatgcactga taccggaaaa tggtattttc ttctgagttc
    27661 attgtatgct ttcaaagtcg gttcggaaac tccgcaaatc cactcatcgg agaaggcttt
    27721 ttcggtatat ttacctaaat tgaagtgagt tggccataat tgtgtgctgt gaatagttct
    27781 gataattttt ggtattttga tgccaaaaaa tgataaaatg cgcaacgtgg tggaaagcac
    27841 caaatccggt aaatcggtat gagaatgaac gatttgcggt ttttctttca gcaggaaata
    27901 taccagccta agtggcccgg gcaacaaact cattcttttt ggagttttag ccagcgtgat
    27961 tgttctgata ttctgcgaaa caagcttttg ttttttgtta agtgcatagg catcgcgggt
    28021 cggaaacagc tcgacaatta caaactcaaa atcatcctga cgggctaact tacaatgctc
    28081 ggctatactg agtgccacat tttcagagcc acccaatttg atggatgcaa tcaataaaaa
    28141 aattttaatc atgtgtcagt ttcctccatt cgttacggaa ctgctgttcg gaatttctca
    28201 tcagcgatgg tttttttgtc agatcagcat ctccaacggg ttcgatttcg gccagtaaaa
    28261 gggttaattt ctcaatagtg tcatcaccat tcaatggatt aaatatcagt ccgttgtcag
    28321 gtcggatcag acacgaagct cctgcgtatt gagagcaaag caccttcaga ccgaatatga
    28381 gcgcttcgtt tactaccgca ccaaaggttt cggatacgct cggcaaaacc agacccgatg
    28441 cacataaata ccaggcataa agttcctcac cttcaaattt tcccggcaat atcacctttt
    28501 cttctaaatt tttttcaaga ataatggctt tcaacgaccg gagttccggg ccttcaccta
    28561 ccactataca cacgcaatta ctttgctcat gcagcttatt acatattact cgtagaaatt
    28621 gtggcaatgc tttttcggga ataaaacgtc caacgaataa cagcactttt tttccattta
    28681 gttgatattt ctctgcatat gtttttgcaa tttcttcaag tttctcagag ttactacgca
    28741 gtctttcagg gttttggagc agtggtgaaa taattagttg attttcatgc agttgaaatt
    28801 ctttctgata aaaaagcgaa acttcagccg aattaaccac taaaaaatcc atgtatttca
    28861 ccgaatattt tcttgctttg gcgcgtatgc ccgattgtac gtgatggcaa atatctatgc
    28921 tgtcgtcaat catggagccg attttttgcc ggattaagcc cagccgtttc agcagaataa
    28981 gatattgagt ggtaaatgaa aattcataac cagaaattat gtcgggattt attgatttga
    29041 tttttttcag cataccgtac ctgaaaaccc tgcctttgta gcggggacct gttaacagcc
    29101 acgaaaccct gaaattcagt tgtgaaatca gcctactctg atccattttg tagctccaca
    29161 agttatcata cagaaaaact acttccagtt caaaaagcag actcagggca ttgaactgat
    29221 ccactctgta gggtgccagt gccggatgaa atataagtag tttctttttt gccataagtt
    29281 gtttacactg ttcagctaac gtatagccgg ttatttatcg attttatatt tattccaaag
    29341 agatggaaac agtactttga gccagagtag tacaaatgaa taaggtaaat aaagtggaaa
    29401 atgtttccgg atgtagtaca ggtattcctg ataagccaga cctgtagggc ttttcagata
    29461 cgctatccgt tcttttagcg gtacttcccg cggtttccag ttgtgaccat ggtcatcgtc
    29521 gcacacaccg catatatgtg gtgcctgcaa cacaggaatt ttcattttcc gtgctctcag
    29581 cgagtaatca taatcggcaa tgccatgtgt aaatcgggga tcgaacatcc cgatttttcc
    29641 cagcacttct tcactcaccc acaggatgtt agcatttgtc aaatcgcata cctgtggact
    29701 atcagaaggt ttcaggaatt ctgttttaag tataaaatga tttgatttaa taattgttcc
    29761 gccataagtc attttttcac tgtttttttc tttggttgca cctgtgtaaa tacctttcat
    29821 tccgtaattg tccatcgaga atttatcggt atggattaaa tgatgcagaa aaccggtatt
    29881 tattgtcaca tcatcgttga ttaaaagata tgcatcatag tttttaacct tcatggccgt
    29941 tttccatgcc agccgcattc ctcctgccca gtaaagctga ccgctgcccg tgataatttt
    30001 cactgatgga aactgagctg ataccgcatc agctgttccg tcggtacacc catcatccac
    30061 cagatacact tccatatcat atcccacagg caattcagca gcatacaggg aggtcagaca
    30121 gcccagcgtt tttgctttgc ggttgtgaca ggtgagtaaa acggctatgt gttgaggttc
    30181 cattattctt catttttttt agtgtcattc tccggattga gatagcgttt atcaaaaata
    30241 ccccgaaccc agattgttac ttcacggtct gtcatgcccc ggaatgtaaa tgagaagcac
    30301 aaaccgagca ttatccagag catcagataa ttcaacgaaa aattattaac atcttccacc
    30361 caggcataaa gccagcgaaa tgcaacgtaa agtcctatga ttttggcata tatgttttcc
    30421 gagcggttga ccgcaaggaa ggaagcgctg aagaatatcc aaaaataaag tatcacccca
    30481 ataatcccgg tccaggtgaa aacattggta attgccacct cattttccag tcgttcgtac
    30541 tttcctgtca gttcaaacag tatgtcgccg aaagcttccg actcgtttcc ccgggccgga
    30601 gttcggccaa aaagccagta attatttttt tttgccgatt gaagtacttc tacatatatg
    30661 aacgtacgtg tatcagtttt caaatctatt tttacgcggt taccctgttc gtccacaccg
    30721 gttccttcgt agctggtttt gatgtattca tccattttga aaacattaaa aataccggtg
    30781 atacccaaaa cgaaaaaaaa tatgggtaaa atcagtaagg ccaaccgaat tatttccatg
    30841 gttttaaccg aaagtgtatt tctaaaataa taaaccagca gaaaaatcaa tggtatggca
    30901 aatttgatga tagtgctgcg tgctcccaaa tcggccgtca gaaccaccgc tgtaaaaaaa
    30961 atcagaatga ttttctgacg aagcgaaaat gccggcaaaa acaacagtaa aaaactgacg
    31021 ggtatcagat aaaatccgta ggcatctgtt cggattaata aggcaaaaat caaaaaaagc
    31081 ggcaaaccga ttttaacata gaaagagagt acgtactgca ccattacttt attggtggca
    31141 gagtaggcta ccacgggcag cagcaatgcg aatgcgttgc cgataagtcc tttccactcc
    31201 caatatccat cggccacaaa catgccccgc acaatgcaga taatattcca aagcaggtaa
    31261 atctccacaa tcctcaggtg tgcggcattt ttcctgtcga aaaaataaaa attggacatc
    31321 aataaaacaa ttaaaacagc aaccgaaatc acccaccaaa aagttgtact tttcagatag
    31381 gtgagcaatg ggagtttttc gttggggaat gacaacacgg aataaaccgc tacaagtagg
    31441 attgtaaccg ggagtaatag tttaatcagt tgttttttag tgagttgcat tccgtgtttt
    31501 ttttgctgaa aattcaatat gattgagttt cactttacag catgattttt tattatctca
    31561 gcatagatat tcatatactg gttggtagtt tctacaatat catgacgttt cagcgcaatt
    31621 atttttgcct cctggcttaa ttttactgct aactcatcat ctgaaaatat ccggtcaatc
    31681 tgaaatgcca gcatggctgc atcgcccgaa ggaaaaaaga gtgagctttc ctcatttttt
    31741 accatcgaca ttacaccgcc taccggtgat acaactgttg gggtgccaac caacatggcc
    31801 tcccccagtg aatttggact attttcagca taggatgtaa gtgtaaatac atgagctttg
    31861 tggtattcat cagccatttc ggcagcactc aatcttttat aaaaaatgat attttctcca
    31921 agttctaaac tttttatttc ttttttcaaa taattggcat aatcttcggt gaataaaaag
    31981 tcccatattc ccgacgattt ttgcctgaac gtagcgagtg gtacggctat acgaaggtct
    32041 ggatatttgt ttttcaatat gtcagcagcc tttaataata catgaaaacc cttgaggggt
    32101 tcagctaccg aggagataaa tatccgatgc cgctgacagg tcttgatatc ccatgtttga
    32161 gaataaaaag gggttctcag taattcttca ccgtgataat agtgagctgc gggattaaaa
    32221 gctgccagtt gcgctttatc ccatgaagtt ctgccaataa aatattcgtt tatttcaaat
    32281 attaattttt caataacgga gtatttctcc caactccgga tggctgcatt tactccgcct
    32341 cttccaatcc tgtttttgat tgatttatag tgatttaaat tgagatttgc cacactgcac
    32401 ttcatatatt ccaaacaagg ctgaattatt ccctgaatag agcacacaat tggtatttga
    32461 ggatcaacga attgtcgaag cagcccgaaa tttcgttcga tgccatgaat atgtataata
    32521 tccggtttga aatcacgaat tgcagatatg tatttatctg caagtttttt ttgcataccc
    32581 gtcatattat cattttttgc gaccggtatt gaatagtagg taacattttc aatttccatt
    32641 ttttgctcct caccatcggt taccggggca atggatccca gtttcacctg ttcaacatcc
    32701 ctgagattgt aaaacaaggg agtagtccag gaggcaccct gagttggttt tccctttaaa
    32761 taaggcaaaa acggttcaaa tacgtcgtgt gtaatccaga gaattcgcat atttatattt
    32821 ttaactgctt acaataaatg aaattttttc tacacaaaac accttgaata ccagtaaaac
    32881 aggctgaatt tattcaaaac ggcaattcct ttgtgaccaa aaaatccctt ataacgatag
    32941 tatagccaac attcataata aaccgacaaa tcgatgggat ttaacaggct gaggtgtggt
    33001 tttctgtatt ttttgaatat ttcataagcc tccttatctc ttttatcatt gatttttttg
    33061 ctgctgctgc gcgaaatggc atgttcgtgt atgcggtact tcaccgtgct tttatccaga
    33121 taaaaaattt ttttgttaaa agcatttatt ttgaatatca gacacatatc atcgtaaatt
    33181 ttgaactcat catcgtaata acctatggct gccattgtct cttttttgaa gaaaaatgtg
    33241 ggagaattga gaaactcggg ccaacgggca taatttttca attgttcctg tgcggtcttc
    33301 cctttgagga aatgcctcaa tccggtgtcc tgcgtggttt tatgcagcaa cgggcggctt
    33361 tgattgtcaa tttcctctaa atctgaaatt ataaaatcag cttcgggatt ttcacctgca
    33421 aaattcagta aatcttcaat acaattatcc tttaaaatat cgtcgccggc aataattttt
    33481 atccattcac ctgcagatgc tttaattcca cggttgcaat tcaatgaaat acctgagttt
    33541 acgggaacag taatcaattc cgttctgata aaacgatctt tattcttatc aatccagtct
    33601 ttgcaaattt ctactgtatt atcgggtgag caatcgtcgg ttacaatcag ctcgacattc
    33661 ttccaggtct gcgctttggc gctttcgagc gtttcgagca cataggccga actgttgtaa
    33721 gtgattatta caatggaaac aagtggttgc aacgtgtttt tcatacttat caggagtctg
    33781 ttgttttgtt tctgtgagta aattctttaa tgatttcctt tataccaatt cttttttcca
    33841 attcgttcaa agaataaccg cctgatatta acaatagaac tgttccgata atgtacgggt
    33901 aaggctgttg aaaagaaata acggctaaaa agcaacttaa tgccagcgaa aattgaataa
    33961 taaatacttt aaggaaatca ctgttaaacg aaaattcaaa tttcagcttt gctatcacaa
    34021 atacctgaat aagataaacc aaatatccga gtaaaaaaga gatgccaagt cctgtcagtc
    34081 caaagaaata atagcccgaa acatttaata tcagcatata caaattcccg gataattcat
    34141 tccaaaagaa caacttacta gtcccttttg ccagaaaaat aaaagcgata gcccagcttg
    34201 ctgctttgaa aaacataccc agtgccgccc aataaaccat ggtattaaca gcaatgaatt
    34261 ttgaactgta aagcaggata atagcccagt tgataaaaac aagaaagccc acaacaatcg
    34321 gtgccaaaat cagaagtgcc acttctgctt gctggttaat ggtttctttg cacaatagat
    34381 tattgtgagc tattgccgaa agacgagggt aataatcagt agccatagcc gtaaatatca
    34441 accccacata cgtattgata agcgtaaatc cggcattata aagccctacg tcagccactc
    34501 cgccatgccg gttgataaaa atttgaagta aataggacac acccagcgaa ataaatccgc
    34561 tcaaactaat cataaatccc atggtcacca tattccgacc tacagcaata gttcttactt
    34621 tggaaactct aaccttttgg agtgaaattt tattcgagaa aaacagtgca atcaaaaatg
    34681 aggttaccga agtcagaata attccgggta ctatcccgtc aattccccat ttgtagtaaa
    34741 gcggtactgt aattagtaaa cctaataaac taccaaataa attcgcctgt gccagatatt
    34801 gcagctttcg catgccttgc aacaagacca attgaccgct tgtaagctga ttgaagagta
    34861 aggatataga aatccagata taagccagcg tgtactcact gttgccaaaa gtgagccggc
    34921 taagccaggg tgaagctgct aaaactacga ttgtgcccaa cgtgccgctt atccaaacta
    34981 atctgcgcag tacaatggtg acggttgcaa tgcgtgtctc attttcagtg gttcgggctt
    35041 cggctatatc ttttaccgca ctggtgccaa gtccgaaatt ggtcaaccca ctgattaatc
    35101 ccaatgtgga agtaagcaat ccaacaattc ccataccggc aggccccagt aatacagcta
    35161 caaatttaga gcgtataatc tgtattaaaa tttgaaacac ctgaactcca ccaaaaaggg
    35221 aagtcgctgc cattatctga cgataggatg attgttgtgt attcattcac aaagtgttta
    35281 accggttgat aatcatgtaa ctgaaaacga atttacaatt tttaccacca tttcagcttc
    35341 tgcatcggtc agcacctgac tgattggcaa actcaattct tcccggtgta tttgttcggt
    35401 gatgggaaat gaaagattgt tccactccct gtagcatgcc tgcttgtgag gaggaaccgg
    35461 gtaatgaatt atggtttgaa catctttttc agccagatat ttctgcaatt catcccgttg
    35521 accgcaccgg attggaaata tatgaaatac atttgcatcc caatctttta cttcaggagt
    35581 aataattgcc ggattagtga tattctcaat ataatagcgg gcgatttctt ttcggcgctc
    35641 gttgtccttg tccaggtgtc ttaattttac ttgtaaaatt gctgcctgaa tttcgtccag
    35701 gcggctgttt aatccctgat aatcgaaaat gtatttgtgc ttagaaccat aattggcaag
    35761 tgtacgcacc acctcagaca gctgatcatc gttggtggtt actgcaccgg catcgccgaa
    35821 agctcccaga ttttttcctg ggtaaaaact gtgtccggct gcatcgccca gcgaaccggt
    35881 agttattccg ttaaatttac atccatgcgc ctgcgcgttg tcttcaatca gtttgagatt
    35941 atatttttta caaatactgc caatcgtatc ggtatatgca cactggccgt acagatgaac
    36001 tatcattatc gcttttgtct ttggcgtgat agcatcttca atttttctgt catcaatctg
    36061 atacgtatta atgtccggtt ccaccaacac aggaaccagg ccattgtcag taatagcaag
    36121 gattgaagca ataaatgtat tggccggaac gatgatttcg tcgcctgtat tcattagtcc
    36181 catttcaatg taagcccgta aaatcagacg tagtgcatcc aacccgttgg ctactccgat
    36241 gcagtatttt gtccctataa aaccggcgta aactgcttcg aatttttgat tttcgatacc
    36301 cgtaagatac caaccggagt ctataactcc ggccactgcc tcatgtattt ccgctgaata
    36361 cttttgagtg attttttgca gatcaagaaa ttttatcatc acattgagtt tagttcatga
    36421 aaattgccat ctttatcctt tttatgcata tccagcagta tgccatcgtt ggtaataaat
    36481 cctttttgcc gggccggatt tccgtaccac aaggtgtttc gtggaatatt gcgggtaacc
    36541 acactcccgg ctccaataaa tgcatattca ccgatttcta caccaggaag aatagtggca
    36601 tttccaccta ttgatgctcc tttttttatc aaagtttttt tcaattttgt gtgatcatag
    36661 gttttggatc gtggtacggt ttcgtttgtg aatgttgcgt ttggtccgat aaatacatcg
    36721 tcttcaatgg ttataccatc ccacaactga actccggatt ttatcgttac attattacct
    36781 atttttactt tattttcaat caatacatgt gcacaaatat tgcagttttc tcctatttcg
    36841 gcatcgggaa gtatcacgca gaattgccag attttggtgt ttttaccaat tttagtgctc
    36901 agcacatcgg agaggtggct tatcatatgt ttttgtattc aataaattta tcatactctc
    36961 taatgtaatc ttccgccgaa tattcggtgg aagcgagtac cagacatact gaacctgatg
    37021 aaaaattatc cagttcccgc cagataccgg gtactaccag caaaccttcg tagggtctgt
    37081 tgattgtata ggttcttttc tgaaagccat cgtccagctt tacgtcgaaa cttccgctaa
    37141 cggctacaat aaactgtttg agttccttgt gtgcatgacc gccccgtgac tcgccacccg
    37201 gaacatcgta cagataatac acccgtttca cgctgaaagg gatggttttt ccgttttcga
    37261 tgacgctcag atttcctttt tcatgatgat gcttgtcaat ctcaatgatg gaacaatcat
    37321 atacggttgt ttttctcata ataccgaatt tttaaataac tcgtaatcaa aaatgtaatc
    37381 gtccggatca tatttggctg aagccagtat cagtgccagt gaattggttg aaaaattctg
    37441 catttgccgc cacactcctt tgggaacgta caatccgtaa tacgagcggt tcaacgaaaa
    37501 ggtctttctc tctttcccgt cgtccagaat gacatcaaaa ctgcccgaca gggctacgat
    37561 aaattcctgg tttaccttgt aggcatgtcc gcctctcact tcacctcccg gcacatcgta
    37621 tatccagtag gtacgtttta ttttaaaagg aatatttttc aattcctcta ttaccgatag
    37681 atttccccgg cggtctaatg ttttggggag gtcaatgatg cgggctccgc gtagtgtctg
    37741 cattttttta attttctaat gagaaaaatt acaattagcc ccgaatgtca gctttttttt
    37801 gtttcgcttt catagccgta agtgtgcctg gttttgccgt aaccatagcc atatccgtat
    37861 tttttgccat atccgtagcg ataggtgtgt ttgttaaggt ccacatcgtt cagcacgaag
    37921 tacatatttt tgagtttttt ctttttatac aaaatggtag catcttcaat atcttttttg
    37981 ggagtaaatt cagcccgaac tacatataat gtaacatcgg tgagcctgtt gagcaggaaa
    38041 ctgtctgaaa ccacgccaat gggagctgta tcgatgacaa tataatcgaa gttttgccgc
    38101 aaatcgtcaa tgagtttatc cagcgccggt ttggccagga gttcgttggg gttaggagga
    38161 atagttccgg cagtgataat agatagattt gggtgaattc cagttggttt cacaagttca
    38221 ctctgactga tttgtccgga aagatagaga gtaatgcctg ttttattttc gatacccata
    38281 tattcactca gtttgggttt acgtatatcc agttcaatga tcagcacctt tttgtcgagt
    38341 aatgccagac ttatagccag attgatggtc acaaaagtct ttccttcgcc gctcacactc
    38401 gatagcaggt tgatcacttt tttatccgga tcgtccatca caaaaagaac atttgccctg
    38461 agaagtctga ccatttcggt gaaactgtcg gtactgtttt ctttgaccaa cacattgcct
    38521 gtttgatctg tttttggaat ttcgcccagc accggcacgg tggaaaatgc ctccagttct
    38581 tccttatttt cgatctgata gcgcatcaaa tctctgatct ttattccaat tacgggtatg
    38641 agcaggccaa tgagcaatga ggagaacaga ataattttct ttcgtggagc aacgggacct
    38701 gcataccgaa ccgtgtcaat cagtttggtg ctgggagcaa cagccgacat attcatgtat
    38761 ttttcctcct ttttttgaag taaatataaa aatagtcctt ctttcactcc ctgttggcgt
    38821 tttatgtcag tgtattgccg ttcctgacgg ggtaccgctc tgatgcgtgc actggtttga
    38881 tagtattgtg acagaatgtc cctgcgtgca atctgcaggt tgttctcctc gttttccaaa
    38941 ctggatttta cagtgttata agtattttca attttattat tcagatctac catggcctga
    39001 ttactaccag atgcaatacg tgataatctg ttcttatcca ggaccagttt gttgtaatta
    39061 ctgatcatat cgttcagacc actgcttttt attcccgaat tggcgggaag aagctggtcg
    39121 tgactttgat tattctgaac ataggatttc aggttggaca caatacccaa ttgtgtttcc
    39181 aattcaagtc ttttttgttg cgcatcggcc gtttgagcat tgtagatgtc tgattgagct
    39241 gccagatccg ttattccctg tgattgtttg tagttttcgg cctgcgattc tacggtatta
    39301 agttctttgc tgagtgagtt gagatgctct tctatcagtt gtgaggtctt attggccatt
    39361 tccagttggt cattcatttc ttcccggtta tatacttcga tgaattttct cagaaaatct
    39421 ttaccgagtg caacatttga acttaccatt gacaaattca ctaccgatga tccttttgag
    39481 gccagttcca tcttgatttg tttcaaaaac tgatcggcta ttgtcatcgg attttcaaga
    39541 tttacctcta tctgcatatc atcatgcagt ttcagattgc ttttcctgag gtttattctc
    39601 ccaaagggta atttgacatc tttatccgaa attatggtat taaccgtgta aggattttcg
    39661 ttatacgtac cggtaaattc gcacttccca tcggggtgaa ccagtacctg aaagctgacg
    39721 ggttcgttaa aatttttcag aatgctgtcg ggtaaagtga ccaggatggg gctttcatct
    39781 ccgtaaagca catagctcct gtatttgcct aaaccgggaa tgagtttgtt gattcccaac
    39841 ttttgaatta aactcaaagt gctgatttga taatacgtgg catagacccc aagttcacgt
    39901 accactttct caaaaatcaa tgagtttttc aataattcaa gctcattatc tacactgttt
    39961 ttttgtgtaa tgatactcat gtccttgaat acgctcattt ccaccgatcc actgcctttt
    40021 acgtcatctt taatcaatac agatgttttg atgccgtaac ttggcagagt aaatttaaga
    40081 tatgtagcag ctactgctac acatactatc agcgaaagta caaaccactt ccagtaagaa
    40141 atatacttaa tcagtattcc taataaatca atgggtggct ccggcaacag attgaattca
    40201 tcttcctttt cagagtattt attcatatta ttttttttta ctcattgtat cacctaaaaa
    40261 ttcaattaat aaacctgcta ttatttttta taaatggaat acaccgtcaa aaccaggctt
    40321 gtaagtgtca atccaacgga taatgaggaa agtctaaaag tttcggcggc accgaagttt
    40381 gctgaatttg atttcgattt attcggttct acataaacca catcattctg ttccagaaaa
    40441 taagccggag agttgattat atttttatca ttaagattaa gggtgatgta tttttttatt
    40501 ccctcggcat cttcccgcag tacctttacg ttgtcgcgac gaccgtatat ggtcatatcg
    40561 ccggctaatg ccaggccttc gagtatcgtc attcggtcat taccggtttc gtattttccc
    40621 ggttttagca cttctccaag tatatttacc gagtagttat tgatacgtat ggtaatgatc
    40681 ggcatttctt tggtgaaagc tggtgccagc attttctgaa gtgttaattc caactctttg
    40741 cgtgtaagat ttttgacctg aatttttccc aatacaggaa aatttatata cccgtcatta
    40801 tcaaccaggt aattttgcag ttttggttgt gaataaagtg agccggcatc agtgggtgtt
    40861 tctgtttgcg gggtaaccag attatagatt cgcgaagctt cgggctccga actcacaaca
    40921 gtaatggaaa gcatatcttt tggtttgata cgggcatcaa acagacccga agatgggttc
    40981 gtattaaccg ataatttgga tttgttgtcc gatttttcaa aatacgcaat gttttttacc
    41041 cctgagcagg aactgataaa aagtatgcct gccaataaaa acatattctt gtaagttgtt
    41101 ttattcatcg ttggtttttt tatttgaaaa gttagtataa ttctttcacg tattattaac
    41161 cgagtgcttg ttgtttgttt taagcattat caggcagata tgtttctttg tgcttagtat
    41221 tgctcctttg gggcaatttt attttttatg atagaacacg ttaaaaccat taaaagttta
    41281 gatctgtatt ttattataaa tcaaaagaat ttgaaaataa tatgtttttt aatataaatt
    41341 cttagcagtc tatataatca ctgaaatgct gaacaaaggc tctgaataat ttgtttttta
    41401 aatgataaaa aataggaatg acggtcgaac tgattaatgt ggaataagtg tctgcatttc
    41461 agaatgatat gaatttatat ccggtaaaga tgagcaagaa atcaacaatt attccggttt
    41521 tatttttttt aattcattgt tttgtagccg gacagggatc agcctataca ggattgtata
    41581 aaaaatctcc ctc
//
LOCUS       DAOPZK010000002        33430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1225_length_33430_cov_21.682067, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000002 DAOPZK010000000
VERSION     DAOPZK010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 33430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33430
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1225_length_33430_cov_21.682067"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKH68_00170"
     CDS             <1..195
                     /locus_tag="PKH68_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNX11379.1"
                     /translation="KEVMEEYGQVDSCKLITDRDTRRSKGFAFVEMADDDAAKKAIAE
                     LNGAELDGRTMVVKEATPRS"
     gene            496..1080
                     /locus_tag="PKH68_00175"
     CDS             496..1080
                     /locus_tag="PKH68_00175"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNX11380.1"
                     /translation="MNHDTEILIQPLKNGSVKAFDALYQLYSARLYNFVLKISNGDTY
                     MAEEIVQRVFIKIWEERSLLDTQKSFNAFICTIAKNMLMNEYKHQMVEFVYQDYILQI
                     NKDESSDGVDKIEYVFLEKYLNTLIEQLTPARREVYIMNRIDKLTVKEIAQRSGKSEK
                     TIEKQLSEANEFIRRQFSKHYDKLFSLLIIPFLV"
     gene            1583..2599
                     /locus_tag="PKH68_00180"
     CDS             1583..2599
                     /locus_tag="PKH68_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HNX11381.1"
                     /translation="MDQQITALFRKYLSNSATADEMKELDEWIASNEDFHDWLENQVD
                     NSSAEMDAEKQADILAKIHEKIAVQSKQKFVLPGWAKTVAAVALIVLMAVSAAIYFRS
                     NQPNMIQYAEIGALRGQKASVTLPDGTKITLNSESTLKYSTNYNQSDRAVELVGEAYF
                     EVAKNKKIPFVVKAGKLEIEAKGTAFNIKAYPTDNSISTTLTEGKIEVKTPVDVLNMI
                     PNERMEYNNTDQTFRKLTLTDAEGSIGWLNDELSFENATLAEVVANFSRIYNIDIQFA
                     SESIKEQRFTGKINNNSLLSVLRIISFTSPIRFEQKDSVVILYEVPSEKKLFQVNKNL
                     KNTN"
     gene            2615..>4803
                     /locus_tag="PKH68_00185"
     CDS             2615..>4803
                     /locus_tag="PKH68_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX11382.1"
                     /translation="MKEKKSQLLKSLLRIVLKVFLLVSMISMPVILHAQITVKADNET
                     VRSILKKIEKQSDMTFFYNEALTDLNKKVSVNVKNSSLESTLNQVLKTTGLAFSFQGK
                     TIVLTDKNKIVPATETTKPQSNITEKGRKVTGVVRDAAGETLIGVAVAVHGSANRVAT
                     NVKGEFELQNVAPDATLDISYVGMKAQSIPLAGKTTIAVVLSDDTKLLEEVVVTAMGI
                     ERKSKSLTYATQKMNNDELMRVQDANFINSLQGKAAGLSITPNAGGAGGSSKILLRGN
                     KSVLGNNTPLIVVDGVPLSNPVKGQQGIQGGQAMGYSFTTEGSDALSSINPDDIESIN
                     ILKGANAAALYGSAASNGVLMITTKKGQDGKLTVGVTSNATFENPLLLPQLQNIYGAD
                     VNTFAGTVAGESWGRKIADMTTQETAVKNLTNVPQNYINDFFKTGNTFNNSVSISGGS
                     KFVRSYFSYGNTNSGGMIDNNKFNRHTISFRQNYSLFKERLKIDASINYVNQNTKNRP
                     GGGTSLNPLFNLYTSPRNVDMNYYRANYKINDGTWMSNNYDYYQLSNKSYIWTSGQTE
                     LKGPKQNWVYTSADQNNPYWLINMINRTDEQNRVYGYLSGNLKLMDGLTAQARLSIDR
                     SGSTAKTETSATTQFPSTMLDYGEFRQNISNTSEFYLDWMLNYNKEFGDIAVSASTGY
                     TAHQITGNSQSLLISRATAEDALRRVIPTAVNLFDPRAGITGGRDYG"
     assembly_gap    4804..4903
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4904..6004
                     /locus_tag="PKH68_00190"
     CDS             <4904..6004
                     /locus_tag="PKH68_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX11383.1"
                     /translation="SVGVNLLTGASTPSPYAYFENPIPEKTKSYEAGFDASFFKNALT
                     WDMTLYHSSMNNNYLLVGVSGKQKPVNTGIIRNMGVETTMSYSMNLAKNLLWKTGVNF
                     AYNNNKIMKTYKDKNGNESLIEQQIGFGGKVQIKYREGGSYGDLYVTDFARDENGKIK
                     LTNEGKPSLSSDKFGVFMGNMNAPYQLGWNNTFSYKGFTFYFLADGRIGGKVISFTEA
                     FLDFEGVSARSGAARQAYEADKGNLTYRYKDSQGAEHTVPGMLMPDGQLTSIEAYYKG
                     IGGDVNASQYVYDATNFRLREVSLGYTFRNLFGPAKNLSLSMVGRNLFFLYNNAPVDP
                     DTSISTQNSLGSVDVFNMPSSRSVGLALTVTF"
     gene            6028..7845
                     /locus_tag="PKH68_00195"
     CDS             6028..7845
                     /locus_tag="PKH68_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNX11384.1"
                     /translation="MKSTILYICAALLAATTLSSCLFNEDFVGSEFTAGTIEGETAVH
                     LGKADNINYQRKITEAGLTSALETMNKTKLLGQMLSAQYALRGGKQGNSPGPHAYQYQ
                     FSLETDNYAGYLCLPQDFGGRMQSTYYNSQDFNGGAMGSFMEMKNFIVPILNHPQIDS
                     IPEIKAISLLIYDISAQEVADIYGAFPYVNYKANRQDHPFVYNPLDTIYATIVNNIDT
                     IVSCLQNYPNRPQWYQAKVDGILKQYDRVSPTTGDKIQNWIRLANSLKLRMAMNIVKV
                     EPVLAQKWAEQAVQSGVIETSAQQFGVSPQIIGFSHPLIEISNSWNDTRLNASFESIL
                     KAYDHPVLSFLFTKNSHPIINKDDPSKIYPAESGYVGLRSGIRMLSGQAYNVNFRTAY
                     SRISEAILEMPLFVIKLSEVQFLRAEGALRGWNMGGSAEGFYNLAIENAYSDTELKIY
                     DELNDDFIYFDQTKYRPALANYMKTENARDIDYVDPYSNEYNRKSTLKVGVKWNNGDN
                     NETKLEKIITQKYIAGFPNSFVAWGDLRRTGYPRIFPVVYDDGDGSIPAGDIIRRIPF
                     SGTSQEAIRNDIANTGLRALGGPDKQGTRLWWDVAGANF"
     gene            7899..10922
                     /locus_tag="PKH68_00200"
     CDS             7899..10922
                     /locus_tag="PKH68_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNX11385.1"
                     /translation="MKNKFFTGMLVALLLSLGGFSVNAQTSPTASKEIYNWTPYGDVN
                     MLNLFYDVLKQGRNYPTPQEIRNTFGFDMEFSRSHVRPHAVMYNQAKQINPTINPKRK
                     IWMNIPGGIGKGNGGYPSSLFNNDVYSLWQYTHLFGSWNHGIFQAPGSWADAAHKHGT
                     DIFSGIKFFESWTPGSGDATYAALISQKEGDKYKYAEPLINILMYMGLDGINYNWEDN
                     SYSNTDVIAFHKELYRIAKEKGFNNFHIGIYTLNSGLTDTNKETLYYGHNPSDKTMDL
                     MLNYSAGNFATSSTAASLTAAKSVNPNELSVYQGVWIVTMDRSWTTMNTDTRKEMNLC
                     LWGEHAQNRFMSYNNGTDAFNIQENYQKLLERGFSGGNRNPAKLPTLANTGNNWELSG
                     SKEPLSTFGGLASFVAERPTVQGKLPFYTNFQLGNGERYNYKGKKTFGNWYNMGAQDY
                     QPTYRWLVYEANTKTVSTAIQPGYTHRDAYTGGSALELKGNTTTSGTDIVLYRTDLEI
                     SAGNPQVSVAVKSYKEGTNPTDLYVILKKKDTDTWTEIPVGNTTDKTWQEMTLPVSGF
                     TAGDHIEYIGLRVKAASAAPYQMYVGKLALTDDREVKEVAPIEDLIAEVKRETQSTMA
                     VKLNWKVSPIAASPRADQTGLVYNDEANVDHFEIMYKNGVEGRISELGRTSTWSAYAG
                     DIEFSDNGTLDDPYIGVRSASVDGKNYSPVVWINIPRAEASQLPVFAKDGYCATEINP
                     DSEGANVARVQRYLTEVKTTGLSENLNYTADAPVADGTQYKNATDKSFTAAQGQTFDF
                     FFKAFDASDGLKWCFAKAYIDWDKNGDFDAVNEIVFDLGAVRAGTVEFQTTGVTKSFT
                     VPANANTGQTRMRIVFSDAWFTHPGPCGQTAKGFSIDFTVNITGTNPPVIAVDKHDQG
                     IADEPEKARESGSAVENVYTNPGEFSTFYPNPAESFISFKNTQQMWIYSLDGKLIFTQ
                     KDNLDNVNISQLNNGLYVVKMLNNSVVRSAKLQKK"
     gene            11257..12174
                     /locus_tag="PKH68_00205"
     CDS             11257..12174
                     /locus_tag="PKH68_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNX11386.1"
                     /translation="MEKIKGLINAPFTPFYENGEVNFEPIEAYANMLVKNGLKGVFIN
                     GSSGEGYMLTYDERIALAEKWVSVAPEGFKVIVHVGSTVVKDSNKLARHAQEIGAFGI
                     GAMASPFPKVGRIEELVKYCEEIASGAPQLPFYFYHIPALSGVFLPMLPLLEAVDGRI
                     PNFAGIKYTYESIYEYNQCMLYKGGKFDMLHGQDETILPVLAMGGAQGGIGGTTNYNG
                     RELVGILDAWEKGDLETAREKQNFSQDVINVIVNYRGNIVGGKRIMKLIGLDLGKNRT
                     PFRNMSDDEEASMKKELEAINFFSRCNKF"
     gene            12232..13407
                     /locus_tag="PKH68_00210"
     CDS             12232..13407
                     /locus_tag="PKH68_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNX11387.1"
                     /translation="MREYIEKWAKTYKDDLTENILPFWLKHGLDTVNGGFYTCLNRDG
                     SLMDTTKSVWFQGRGGFVFAHAYNTIEQKPEWLAASKSAVEFLEKYCFDTDGRMYFEV
                     TAEGVPLRKRRYLFSESFAAIAMSEYSIASGDQKYAHKALDIFRLILKYKNTPGLLEP
                     KYNENFQARGHSLCMILINTASCIRRAIQDDILNHQIDESIDHIARYFMKPEFEALLE
                     MVGPNGEFIDTNAGRTINPGHCIETSWFILEEAKFRNWDPKLVEMAETILNWSWKWGW
                     DEEFGGIINFMDCKNLPSQDYAQDMKFWWPQNETIIATLYAYLATKDAKYLEMHKQIS
                     DWTYAHFPDQEYGEWYGYLHRDGSVAQPAKGNLFKGPFHVPRMMMVAYRLCNEILKS"
     gene            13674..14918
                     /locus_tag="PKH68_00215"
     CDS             13674..14918
                     /locus_tag="PKH68_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX11388.1"
                     /translation="MKNSKYYPWVVVGLLWVVALLNYMDRQMLSTMKDAMMIDITELQ
                     KAENFGNLMAVFLIIYGFMSPFGGMIADRINRKWLIVGSLFVWSAVTYGMGLAENFTQ
                     VYWLRALMGLSEALYIPAALSLIADYHTGKTRSLAIGIHMTGLYMGQAVGGFGATVAA
                     AYSWQTTFHSFGLIGITYSVILVIFLFEKRNEQKDIETLHNAGKAPEMKIPLLKGLGM
                     IFSTAAFWVILFYFAAPSLPGWATKNWLPTLFSESLNIPMSQAGPLSTITIAASSFIG
                     VIFGGILSDRWVQKNIRGRVYTGAIGLALTIPSLVLLGFGSSLVAIVGAAMLFGIGYG
                     MFDANNMPILCQFIPAKLRGTAYGMMNMVGVFAGAGVTSVLGKMTDNGNLGFGFAVLA
                     GIVFIALALQLVTLKPKVENMV"
     gene            15001..16626
                     /locus_tag="PKH68_00220"
     CDS             15001..16626
                     /locus_tag="PKH68_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNX11389.1"
                     /translation="MKIKFVSFLLFLAAIFTLSAQNSVFVYEPQIPILIDRTDNVLMY
                     VRLVANDVKTLNTIDLSFGKEVNLKEIRSVRLFYSGTEADQRKDGVYYAPVGLISGNS
                     GKGLAAEPSYSILRDEHKNPSGNIRFNANQNLFPGINYFWISIQMKPKTSLFSNISAK
                     VTAITADNKSLDFELKSQSHTRRMGVGVRRGGDDGVAAFRIPGLVTTNAGTLLGVYDA
                     RYNSSVDLQEHVDIGLSRSTNGGKTWEKMRIPMAFGQYEGLPKGQNGVGDPSILVDKQ
                     TGTIWIVAAWTHGMGNRRAWFNSLPGMTIHETAQLMMVRSDDDGKTWSAPINVTSQVK
                     DPSWRFLLQGPGRGISMNDGTLVFPTQYIDPQGLPHAGIMCSKDHGNTWTISAPARDN
                     TTEAQVAEVTPGVLMLNMRDNRGGSRAVSITSDYGKTWTEHPSNRSALQEPVCMASLI
                     KVEAIDNITGKDLLLFSNPNTTKDRKNMTIKASFDGGLTWKESNQLLLDEDYGWGYSC
                     LTMIDKETVGILYESSVAHMTFQAVKLKDIIEK"
     gene            16652..17821
                     /locus_tag="PKH68_00225"
     CDS             16652..17821
                     /locus_tag="PKH68_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclically-permuted mutarotase family protein"
                     /protein_id="HNX11390.1"
                     /translation="MKFQRNLFFLMVTLAVCACKPTPEKPVRIYITEVESIPGEFGNI
                     SKGVSASFAGLIDERLIVAGGCNFPDTAAAEGGKKVFYKDILLLDGRKWRKIGDLPRE
                     LAYGVSISLPDGLLMMGGRNTDFFSSAVYRLKMDKTSESLTIDSLLPLPVTADNFGGA
                     VLNDVIYVAGGNQDGKPSAEVWSLELKNPVVWKKCPKIPSRPLVQPVVTAAAGNIYVM
                     GGFDVAGDSLMAEVSQAVWMYAPGTAKWTKVADYQTDINQYSLSGGAAVALSDDLILT
                     LGGVNKTVFEEALNRNAYLAKFQPILSDTLTTRLKEEAGNYMFHPEEWYKFNPTIKAF
                     DTKNNTWSSLGQFQQTALAGSTVVANDKVIYVVNGEIKPGIRTPKIWKISRKQRD"
     gene            17890..18336
                     /locus_tag="PKH68_00230"
     CDS             17890..18336
                     /locus_tag="PKH68_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNX11391.1"
                     /translation="MIYSTLTESKHIESLHPKFKQLFDYVKEHDLLRQETGRITLDGD
                     NLFINNVNPQMVSADEQVLEVHQRYLDVHIPLDKAETIGIKPLENCTDLKSEFNSEDD
                     YALYSDKPTNYVTLQPGEFLIVYPHDAHAPIIGEGKIRKLIAKVLI"
     gene            18375..20354
                     /locus_tag="PKH68_00235"
     CDS             18375..20354
                     /locus_tag="PKH68_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNX11392.1"
                     /translation="MIFDLSSKITLERIPKKYFRPSDPLEATRQSRYEKLRTDIYETE
                     KEGAQSVAKEIAALIRTRAEQNKPCVLALPGGSSPLKVYAELVQMHKKEGLSFQNVYI
                     FNISDFYPVDSKHHSNARLINEYLLSKVDLPEDHFFTPANIERTDVLNFCQRYEAKME
                     ELGGLDFVLATIGQVGNLAYNEPGSQTSSITRLMLLDSQSRSDLKQFYISSDEVPVSA
                     VTLGISTILSAKRVVLIAWGETKSNIIKEVIEGKANDNVPASFLQLHKNAQAVLDLTA
                     GQHLTRISHPWLVGPCEWTDKLIRRAIVWLCEKTQKPILKLTNKDYNQYGLNELLALF
                     GSAYNVNIKIFNDLQRTITGWPGGKPNADDSNRPERATPYPKRVLIFSPHPDDDVISM
                     GGTFQRLVEQDHIVHVAYQTSGNIAVGDEEVVRYLSLMSNILEHFDPENDKLKTRYED
                     LRHLLVNEKKSGDMDNPEILYLKGRIRREEARTACHYNGVPLERVHFLDLPFYETGKI
                     KKGDLTEKDVRIVMDLLQELKPHQIFVAGDLADPHGTHRVCLNAALAAIHELKDEEWM
                     KDCRIWMYRGAWAEWEIDHIEMAVPISPEELRRKRNSILKHQSQMESAPFLGNDDRLF
                     WQRAEERNQATAKMYTNLGLASYEAIEAFVQYTIL"
     gene            20503..21459
                     /gene="tal"
                     /locus_tag="PKH68_00240"
     CDS             20503..21459
                     /gene="tal"
                     /locus_tag="PKH68_00240"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNX11393.1"
                     /translation="MNLLEQLKTFTQVVADTGDFHSIEVYKPVDATTNPSLIFAASQD
                     PKYKELIDEAIVYAHNQSPVKSIQLSKAMDKVAVNFGLKILDIVPGRVSTEVDARLSF
                     DTEATVMKGRELIALYEAAGVSRERVLIKVASTWEGIKAAEVLEKEGIHCNLTLLFSF
                     VQAVACAEAGVRLISPFVGRILDWYKKSRGVDAIPANEDPGVLSVKNIYNYYKKFGYN
                     TQVMGASFRNTGEIIELAGCDLLTISPALLHELETSSGKLECKLNAGDAAKSAVERIS
                     ADEKTFRWMMNEDAMATEKLAEGIRNFTKDLIKLENQIAGLL"
     gene            complement(21534..22790)
                     /gene="nqrF"
                     /locus_tag="PKH68_00245"
     CDS             complement(21534..22790)
                     /gene="nqrF"
                     /locus_tag="PKH68_00245"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNX11394.1"
                     /translation="MNTAAIVASLSVFLVVILLLVVVLIVAKRYLVTSGDVKILINGE
                     KEVIAQAGSTLLNTLSTQSIFLPSACGGGGSCAQCRCQVIEGGGEILPTETVHFTRRE
                     IKDNWRLGCQIKVKNDISIKMPESILGVKKWECEVVSNNNVATFIKEFVVKLPNGEHL
                     NFIPGSYIQIDIPAYSLKYSEFDVDERFRGDWDKFNMWDLSCTNNEETMRAYSMANYP
                     AEGDIVMLNVRIATPPFDRVNGGWMKVSPGIASSYIFNLKPGDKVMVSGPFGEFHPVY
                     DSKAEMIYVGGGAGMAPLRSHILHLLKTLKIRDRKISYYYGARALNEVFYMEDFLALE
                     KEFPNFSFHLALDRADPVADEAGVKYTAGFVHQVLANTYLNDHDAPEDIEYYMCGPGP
                     MAKAVEDMLWNLGVPRENLMFDDFGS"
     gene            complement(22849..23466)
                     /gene="nqrE"
                     /locus_tag="PKH68_00250"
     CDS             complement(22849..23466)
                     /gene="nqrE"
                     /locus_tag="PKH68_00250"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNX11395.1"
                     /translation="MENIANIFVKSIFVDNMIFAYFLGMCSYLAVSKNVKTSLGLGVA
                     VIFVLTITLPVNYLLENYVLKEGALAWISPGLASLDLSYLAFILFIAVIAAMTQLVEM
                     VVEKFTPSLYASLGIFLPLIAVNCAIMGGSLFMQQRAFNNIGEATSYALGSGVGWLLA
                     IVGLAAIREKMEYSDVPKPLRGLGITFITVALMAIAFLAFSGLKI"
     gene            complement(23529..24161)
                     /locus_tag="PKH68_00255"
     CDS             complement(23529..24161)
                     /locus_tag="PKH68_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNX11396.1"
                     /translation="MSKLFSAKTKEVFLGPLSKNNPIGVQVLGICSALAVTAKLEPAI
                     VMGLSVTIIVAFSNVIISLLRNTIPNRIRIIVQLVVVAALVTVVSEFLKAFAYDVSVQ
                     LSVYIGLIITNCILMGRLEAFAMSNKPWESFVDGLGNGFGYAIVLIIVAFFRELLGSG
                     SLYGIRVIPQAVYDAGYMNNGLMLMPPMALIIVACVIWVHRSKNKDLQEK"
     gene            complement(24158..24880)
                     /gene="nqrC"
                     /locus_tag="PKH68_00260"
     CDS             complement(24158..24880)
                     /gene="nqrC"
                     /locus_tag="PKH68_00260"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HNX11397.1"
                     /translation="MDTNKNSYTIIFATVMVVIVALMLAFVSGALKDKQTANVKLDKK
                     KQILTSLSVEIDNQDADELFNKIITGGLVINSKAEVLSDSKEQAFEIDINKELSKPLE
                     KRQLPVYIANIDGKTKYVVSLRGAGLWGPIWGYLSLNEDKNTVFGSYFSHASETPGLG
                     AEISQKSFQAEFIGKKVMNNENKFVSIAVVKPGQTAEGMDYVDGISGGTITSKGVDAM
                     LMNCIGQYETFLQQPATGGAKQ"
     gene            complement(24893..26092)
                     /locus_tag="PKH68_00265"
     CDS             complement(24893..26092)
                     /locus_tag="PKH68_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNX11398.1"
                     /translation="MNKLRNLVDKIKPDFEKGGKFEKLHSVFDGFETFLFVPNTTSKR
                     GTHIHDAVDSKRTMIMVVLALVPALLFGMYNVGYQHFMAIGQDAGFWATFWFGFISVL
                     PIVLVSYIVGLGIEFVVAQIKGHEIQEGFLVTGFLIPLIVPVDTPLWMIAVATAFAVI
                     FAKEVFGGTGMNIFNVALITRAFLFFAYPSAMSGDKVWIRTQDTFGFGGGQVVDGFSG
                     ATPLGQIATSASPTIQITDMFGHQFSFMDAVTGFIPGSIGETSVIAIAIGAFLLLLTG
                     VASWKTMLSVFAGGTLMALIFNHFGPDTAAANLPWYYHLVLGGFAFGAVFMATDPVTS
                     SRTETGKWIYGFLIGVMAIVIRVLNPGYAEGMMLAILLMNIFAPLIDYYVVDANVKRR
                     LKRAVNK"
     gene            complement(26240..27592)
                     /locus_tag="PKH68_00270"
     CDS             complement(26240..27592)
                     /locus_tag="PKH68_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNX11399.1"
                     /translation="MANTIKITKGLDIKISGKAAEKISKIPHSAVFEVNPESYHGITP
                     KVLVKEGDTVNAGTPLFHEKSFPEMNFVSPVSGKVREIVRGERRKVMHITIDTDKTIE
                     YKKFSVKKPSDSTADEIRALMLESGLWSFMRQRPYDIIPNPAKSPKAIFISTFDTAPL
                     APDYEFIIRDQLADFQTGVDALSKLTSGNVHLGLKSDASVFSTVKGVIATVFSGPHPA
                     GNVGVHINHINPVNKGEVVWTIHPQDVLFIGRLFNKGIVDLTRLVALTGPEVENPQYF
                     ETISGNNISEIIKGNLKKVSYPLRFISGNVLVGRKITENGFLAPCCSQISVIDEGTET
                     HEMLGWAMPRFNKFSASRLYLTKLLRKKEFKYDARLMGGRRAIIMSGEYDKVFPMDIL
                     PEHLIKAMIAKNIDKMENLGAYEVAPEDFALCEFVDTSKLPLQAIVRYSLDFLKSEVE
                     "
     gene            27845..28327
                     /locus_tag="PKH68_00275"
     CDS             27845..28327
                     /locus_tag="PKH68_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNX11400.1"
                     /translation="MIRDITIADAAQICSIYNYFIENTAITFELTPVEEPEMARRITE
                     ITKNYPWIVYEENGEIIGYAYASKWRDRPAYRFVAETTVYIRQGDEGKGIGSLLYTDL
                     LRKLKMKHIHAAIGCITLPNPASVTLHEKLGFANVAHFREVGFKFDQWQDVGFWQLMF
                     "
     gene            complement(28418..29155)
                     /locus_tag="PKH68_00280"
     CDS             complement(28418..29155)
                     /locus_tag="PKH68_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432906.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNX11401.1"
                     /translation="MKSERIIVMTLLSVALIIASAVIGRSVINRNIRNQAIMVTGMAE
                     RNFESDLIVWQSQFTVKKFNLTEAYAELKKQSETTRQFLLSKGVSKDEITFSAVEINR
                     DFSYTYGPNGNIASSNFDGFRLTQSVKINSKNVSKIETISREITELINQGIEITSLAP
                     EYYYTKLSDLKIEMLAEAAKDAYNRAETMAKNGKGKLGTMVDSNMGVFQIIAQNSSED
                     YSWGGSYNTSSKLKTATITVKSKYLLK"
     gene            complement(29202..30614)
                     /locus_tag="PKH68_00285"
     CDS             complement(29202..30614)
                     /locus_tag="PKH68_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX11402.1"
                     /translation="MKIALTIILFISLSLPSFAFYKKPVVTENQGNDTVMLPDLSKIT
                     LKDSVINFGKIYLSLPYRYGGTTPRGFDCSGFTSHVFNNFGINLNRTSRDQASQLPTI
                     NKQDLQTGDLVFFEGRRHNGIVGHVGIVTETKPNGEFNFIHSSIKRGVTISSSEEQYY
                     GSRFVKGGRALLADNTFVHRDSTITLYSGEKKKSKFELIPKNKKQILVDAKYHKVKKG
                     ETLSSIAQDYDVPISTLKYLNGLKNSKLKRGKKLIISEAVLASTEPTLDNLQVAGSQK
                     VEEAEPSKVILHYASMSPTPSLEIKKTGLNQVSKDAITLRDEQKPIAGITLDKSQQGT
                     GLYKVKAGESLFGIARTHNLTVEKLKSMNNLKSNNITPGQLLAVMVPQSAEIVLVPST
                     SEIKSTASDATSVTSVTPEKKSEKPAVIKSGTIIHLVKKGDTLYSLSRQYGCKVAEIK
                     DWNRNLDENLRIGDKIKINQ"
     gene            complement(30737..31717)
                     /locus_tag="PKH68_00290"
     CDS             complement(30737..31717)
                     /locus_tag="PKH68_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX11403.1"
                     /translation="MSIVKTSQLSIGYAKGSNKTLVQSNLNLELLRGEMVCLIGPNGS
                     GKTTLLRTLAGLQKPLKGNVLIQEKNITEFTQQEKALQIALVLTDKVQIENATVRDIV
                     TLGQHPYTHWLGGLAPEGREKVHEAIRMVHLEHKTNHYLNELSDGERQRVMIAKALAQ
                     DTPLIFLDEPTAHLDLPNRVEIMLLLHRLAHKTGKGIILSTHELDLALQAADRIWLMS
                     STEGVICGVPEDLVIDGSFNRVFQSEAYYFNAANGNFSMNYPLNKQLSVSGDKTRLYW
                     TLRALARAGYSVTADAETHIKISEKGWEINGKILLTIEDLLQELSTFPEI"
     gene            complement(31720..32715)
                     /locus_tag="PKH68_00295"
     CDS             complement(31720..32715)
                     /locus_tag="PKH68_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNX11404.1"
                     /translation="MKPRKTILFILLTVLLAVLFVADLSFGSIRIPFGEAMNVLFGAS
                     SDTINTEILLNFRLPKAITAILAGASLSVAGLMMQTLFRNPLAEPYILGVSSGASLGV
                     ALVMMASAVLPAVFISSGWGMILAAVIGAALVLLLVVGVSFKVQNSVSLLIVGIMFGT
                     IASSLVGVLQYFSNPDAIKLFIMWNMGSLSAVTWEYLQILVPTILTGLLLAIILTKRL
                     NGLLLGENYARGLGIPILQTRVLIVTATGILAGAITAFTGPIAFVGVAIPHIARGIFR
                     TAKHQLLMPACVLLGASLILICDIISQIPTHTLPINTISALFGAPVIIWIILKRK"
     gene            complement(32721..33269)
                     /locus_tag="PKH68_00300"
     CDS             complement(32721..33269)
                     /locus_tag="PKH68_00300"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNX11405.1"
                     /translation="MIKLIVSDEITLKQLEETDAPYIFRVIDSQREYLGRWLPFVPGT
                     KTILDTMAFIWSVTETPEFFRELVFCIHYRNEFAGLIGLKFNPADKANKRTEIGYWLS
                     EPFQKKGIVTQSARMLIDYAFDELELNRIAIKCAVGNQPSKNIPMRLGFIFEGIERDG
                     ELFPDGHFVDLEVFSLLKKEWK"
     gene            complement(33293..>33430)
                     /locus_tag="PKH68_00305"
     CDS             complement(33293..>33430)
                     /locus_tag="PKH68_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HNX11406.1"
                     /translation="LLPSTANDFWESAVARPDLLLGDVIAILHPELLPDYEFVYAEKL
                     K"
BASE COUNT         9845 a         7076 c         7235 g         9174 t
ORIGIN      
        1 aaagaggtta tggaagaata tggtcaggtt gattcctgca aacttattac agaccgcgac
       61 acacgcagat cgaaaggttt tgcttttgtt gaaatggctg acgatgatgc agctaaaaaa
      121 gcaattgccg aattgaacgg tgccgaactt gatggtcgta ctatggttgt taaggaagct
      181 actccaagaa gctaattaac ctgttaagta ataaaaagaa cttctgttgt cgaaagatga
      241 cagaagtttt ttttgccacc ttccgtacct caaaatgata ggttgatatg atgaaaacat
      301 tatcattgat gaagttaaaa acatttactg attgagacag gcaataagaa tataaattct
      361 tgattttgtt gataatattt ttcagtaact cccggtagac cttataaatt attcgaatat
      421 atttcatttt tattaaataa ttatacttat cctgtcgtgc atttttatta cttttgaaaa
      481 atatttctct atgaaatgaa tcacgatact gaaatactaa ttcagccact aaaaaatggt
      541 tctgtcaagg catttgatgc tctgtatcaa ctttatagtg cccggttgta taacttcgta
      601 ctgaagattt ccaacgggga cacctatatg gcggaggaaa tagtacaacg ggtttttatc
      661 aaaatatggg aagagcggag cctgctcgat actcaaaaat cattcaatgc tttcatttgc
      721 accatcgcca aaaatatgct gatgaatgaa tacaaacacc agatggtgga attcgtatat
      781 caggattata ttttacaaat taataaggat gagagtagtg atggggtaga taaaatagaa
      841 tatgtatttt tggaaaaata cctgaatacg ttgatagaac aactcacgcc tgcccgccgc
      901 gaagtttaca ttatgaaccg tatcgataaa ctgacggtaa aggaaatagc ccaaaggtcc
      961 ggaaaatcgg aaaaaacaat agaaaagcaa ctttcagaag caaacgaatt tattcgccgg
     1021 cagtttagta aacattacga taagcttttc agcctgctga taattccttt cttggtataa
     1081 tccattcaat ccttatttcc aaagtcatcc tgacttttta ataagtaaat gttttttgtg
     1141 attttgactt cgtagaagtc acatatttgt agaaaatatg ttcctttttt gatgttcgac
     1201 ccctgtcggg gtcgaataat acaaggtgat tttcagctac aactatgaaa ttccgctgga
     1261 attttttctt aaaaacaaaa agtcaaaatg agtttttcca aagaaatccc tcaaccccca
     1321 aaataaaacg attttctttt cattaaccgg aaggttgttg ttcattcgtt atttttatcc
     1381 cgcccatcta cccggataat ttttcctgca attaattact gcattacaac tttaattgat
     1441 aacccattcc ggttcgctat ccttgtgact ttgtcaaaat cagaacccat tctgactatt
     1501 attatttttt ttaaaaaaaa atcaatatca gggtaagggt ttttagtaat cctgattgta
     1561 taatagataa acaacaatta caatggacca acaaataaca gcattattcc gaaaatatct
     1621 ttcgaactcc gctacggctg atgagatgaa ggaactggac gagtggatag cttccaatga
     1681 agacttccat gattggttag aaaatcaagt ggacaattcg tcagccgaaa tggatgccga
     1741 aaaacaagca gacatactgg cgaaaattca cgaaaagata gctgttcaaa gcaaacaaaa
     1801 atttgtacta cccggctggg caaaaacggt agctgccgta gcactgatag tattgatggc
     1861 agtgagtgcc gcaatatatt tcaggagcaa tcaaccgaac atgattcaat atgcagaaat
     1921 aggtgccttg cgtggacaga aagcaagcgt tactctgccc gatgggacaa aaataacact
     1981 gaattcggaa tcgaccctga aatacagcac aaactataac caatctgaca gagcggtgga
     2041 attggtggga gaagcttact tcgaagtggc caaaaataag aaaataccat tcgtagtgaa
     2101 agccggaaaa ctggaaatag aagccaaggg aactgcattc aatattaaag cctatccgac
     2161 cgacaactca atttccacca ccctcaccga aggaaaaatt gaagtgaaaa cccccgtcga
     2221 tgtgcttaat atgatcccga atgaaaggat ggaatataac aatacagacc aaaccttccg
     2281 aaaattgacc ctgaccgatg ccgaaggttc aatcggctgg ctgaacgacg aactcagttt
     2341 tgaaaatgcc acgctggccg aagtggtagc caatttctca cgcatttaca atatcgacat
     2401 tcagtttgcc tctgaatcca ttaaagagca gcgatttacc ggaaaaatca ataacaacag
     2461 cctgctttcg gtgctgcgaa ttatttcctt cacatcgccc attcggttcg aacaaaagga
     2521 ctcggttgta atcctgtatg aagttccatc tgaaaagaag ttgtttcagg ttaacaaaaa
     2581 tcttaaaaat acaaattaat aaatctaata tcctatgaaa gaaaaaaaat cacaattgtt
     2641 aaaatcttta ctgcggattg ttttgaaagt ttttttactt gtttcgatga tttcaatgcc
     2701 tgtaatctta cacgcccaga ttaccgtaaa ggctgataat gagactgtac gaagcattct
     2761 gaaaaaaatt gaaaaacaat cggacatgac ttttttctac aatgaagcat tgaccgacct
     2821 gaacaaaaaa gtgtcagtga atgtgaagaa ttcatcactt gaaagtaccc tgaaccaggt
     2881 attgaaaact accggcctgg cattctcctt tcagggcaaa accattgtac taacagataa
     2941 gaataaaatt gtaccggcga ccgaaactac caagccgcaa agcaacataa ccgaaaaagg
     3001 acgaaaagtg accggtgtgg taagggatgc cgccggagag acactgatag gtgtggctgt
     3061 tgctgtgcat ggttccgcca acagggtggc aaccaatgtg aaaggggaat ttgaactgca
     3121 aaatgtagct cccgatgcca ctcttgatat ctcttatgtg ggaatgaaag cacaatcaat
     3181 tcccttagcg ggtaaaacaa ccatagcagt agtattgtcg gacgacacaa aattgcttga
     3241 agaagtggtg gttacggcga tgggtattga gcgtaaatcg aaatcactca cctatgctac
     3301 ccaaaaaatg aacaacgacg aactcatgcg cgtacaggat gccaacttta taaactcact
     3361 gcagggaaag gcagccgggc tgagtattac acccaacgca ggtggtgccg gtggttcatc
     3421 caagatatta ctgaggggaa ataaatcggt tcttggtaat aatacaccct tgatagtggt
     3481 ggatggagta cccctgagta atccggtgaa aggacagcag ggaatacagg gcggacaggc
     3541 aatgggttac agctttacta ccgaaggttc cgatgccttg tcatccatca atcccgatga
     3601 tatcgaaagt atcaacattc tgaaaggggc caatgctgcc gctctctatg gtagtgcagc
     3661 atccaacgga gtactgatga ttaccaccaa aaaaggacag gacggtaagt tgaccgtggg
     3721 tgtgacatcc aatgcaacat tcgaaaatcc gctgctgctt ccccaactcc agaatatcta
     3781 tggagctgac gttaatacct ttgccggaac agtagccggt gaaagctggg gcaggaaaat
     3841 agccgatatg accacccagg aaacagccgt taaaaacctg accaatgtac ctcaaaacta
     3901 cattaatgac tttttcaaaa ccggaaatac attcaataat tcggtttcaa tcagtggtgg
     3961 atccaagttt gttcgttctt acttctctta tggaaacacc aactccgggg gaatgattga
     4021 caataacaaa ttcaaccggc acaccatttc attccgccag aattacagtc tgttcaaaga
     4081 aagattaaaa attgatgcct ccatcaatta tgtgaaccaa aacaccaaaa accgtccagg
     4141 tggaggaact tcgctgaacc cgcttttcaa cctgtacacc tcaccgcgaa atgtggatat
     4201 gaactattac cgtgccaatt ataaaataaa tgacggtacc tggatgtcga acaattatga
     4261 ttattatcag ctgtcaaata aatcgtatat atggacttcg gggcaaaccg aactgaaagg
     4321 tccgaaacag aattgggtct atacatctgc cgatcagaat aatccgtatt ggcttattaa
     4381 tatgatcaat cgcaccgatg aacaaaaccg ggtttatgga tatctctcgg gcaatctcaa
     4441 actgatggac ggactgactg ctcaggctcg tctcagcatc gatcgctccg gctcaacagc
     4501 taaaaccgaa acttctgcaa ccacccagtt tccttctacc atgctcgatt atggtgaatt
     4561 caggcaaaac ataagcaata ccagcgagtt ttatctggac tggatgctga attacaacaa
     4621 agaatttgga gatattgcag tttcagcttc cacaggatac actgcccatc agataaccgg
     4681 taattcacag tcgctgttga ttagcagagc tactgccgaa gatgccttaa gacgcgttat
     4741 tcctaccgca gtcaatcttt tcgatccccg tgccggaata accggagggc gcgattatgg
     4801 caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcggttg gtgtcaattt
     4921 gctgaccggt gcctctacac cttcacctta tgcctatttt gaaaatccaa ttccggaaaa
     4981 aacaaaatcg tacgaagccg gattcgatgc ttcattcttc aaaaatgcat tgacctggga
     5041 catgaccctg tatcattcgt ccatgaataa caactacctg ctggtaggag tttcgggcaa
     5101 gcaaaaaccg gtaaataccg gaataatccg caatatgggc gtggaaacta cgatgtcata
     5161 ttctatgaat ttggctaaaa atttactttg gaaaaccgga gttaacttcg catacaacaa
     5221 caataaaatc atgaaaacct ataaggataa aaacggaaac gaaagtttga ttgaacaaca
     5281 gataggtttc ggaggaaaag ttcagataaa gtatcgggaa ggcggaagtt acggtgattt
     5341 gtatgtgact gattttgccc gcgacgaaaa tgggaaaatt aaactcacca atgaagggaa
     5401 acccagtttg agcagcgata agtttggtgt atttatgggc aatatgaatg caccttacca
     5461 gttgggctgg aacaatacct tctcatacaa aggatttaca ttctactttc tggctgatgg
     5521 ccgcattggt ggcaaggtca tttcatttac ggaggcattt cttgatttcg aaggggtttc
     5581 agcccgttcg ggagccgccc gtcaggctta tgaagccgat aagggcaacc tgacttaccg
     5641 gtataaagac tcgcagggtg ctgagcatac tgtgcccgga atgctcatgc ccgacggtca
     5701 gttgacatct atcgaggcat attacaaagg cattggtggg gatgttaatg cttctcaata
     5761 cgtatatgat gccaccaact tccgcctgcg cgaagtttcg ctgggataca cattccgcaa
     5821 tttgtttggt cctgccaaaa atctttcgtt gtccatggta ggacgcaatc tgttcttctt
     5881 atacaacaat gcaccggttg atcccgatac ttcgatatct acccaaaaca gtctgggaag
     5941 tgtggatgtg ttcaatatgc cgtcatcacg ttcggtagga ttagcattaa cagttacatt
     6001 ttaatcaata cagaaaaatt accaatcatg aaatcaacga ttttatatat ttgtgcagcc
     6061 ttgttggctg ccacaacact gtcctcctgt ctttttaacg aagattttgt aggatcggag
     6121 tttactgccg gcaccattga aggagaaaca gccgttcatc tggggaaagc ggataatatt
     6181 aattatcagc gtaaaataac cgaagcaggt ctgacaagcg cattggaaac aatgaataaa
     6241 accaaactct tgggtcagat gctatccgct caatacgcac tgcggggagg taaacagggc
     6301 aacagtcccg gtccgcatgc ctatcagtat cagtttagtc tcgaaaccga caactatgcc
     6361 ggatatctgt gtttgccaca ggactttggt ggacgtatgc aatccacgta ttacaacagt
     6421 caggacttta acggaggtgc catgggttca ttcatggaaa tgaaaaattt tattgttcct
     6481 atcttaaatc accctcagat agattctatt ccggaaatta aagctatctc gttgctgata
     6541 tatgatattt cggcgcagga agtggccgac atttacggtg cgttcccata tgtcaattat
     6601 aaagccaacc gccaggatca tccgtttgtg tacaatccgc tggataccat ctatgccacc
     6661 attgttaata atattgacac aatagtttcc tgtttgcaga attatcccaa tcgtcctcag
     6721 tggtatcagg ccaaagtgga tggtatcctc aaacaatacg atagagtttc tccgactacg
     6781 ggtgataaaa ttcagaactg gattcgcttg gccaattctc tgaaactaag aatggccatg
     6841 aacattgtta aagtcgaacc ggttttggct caaaaatggg cagaacaggc cgttcagtca
     6901 ggtgtcatcg aaacatctgc acaacaattc ggagttagtc ctcagattat cggcttttcg
     6961 catccattaa tcgagatttc aaatagctgg aacgacaccc gattgaatgc ctccttcgaa
     7021 tcaattctga aggcgtatga tcatcccgta ctgagtttct tgtttacaaa aaattctcat
     7081 cccattataa ataaagacga tccttccaaa atttatcctg ctgaaagcgg ctacgtagga
     7141 ttacgttcgg gcatacgaat gttatccggc caggcctaca atgtgaattt ccgcacggct
     7201 tattcccgaa tttcggaagc tatattagaa atgccattat ttgtaatcaa actttccgaa
     7261 gttcagttcc tgcgggctga aggggcttta cgcggatgga atatgggtgg aagtgccgaa
     7321 ggattctaca atttagccat agagaatgct tatagtgata ccgaattgaa aatctacgat
     7381 gagctgaatg atgattttat ttattttgat cagactaaat acagaccggc tttggctaac
     7441 tatatgaaaa cggaaaatgc ccgtgatatt gattatgttg atccatacag taatgaatat
     7501 aacaggaaaa gtaccctgaa agtaggggta aaatggaata acggcgataa caatgaaaca
     7561 aaattagaga aaatcattac ccaaaaatac attgccggtt tcccgaattc gtttgttgcc
     7621 tggggcgatc tgcgtcgtac cggttatccg cgtattttcc ctgtggtata tgatgatgga
     7681 gatggttcca tacctgccgg agatattatt cgccgaatac cgttttcggg cacttcgcag
     7741 gaagcaatcc gtaatgatat agccaataca ggactccgtg cactgggagg acccgataag
     7801 cagggcaccc gactgtggtg ggatgtggcc ggtgccaatt tttaatcaga aatatttgaa
     7861 ctaaattaac acaatcaata ataactcaat tttttactat gaaaaacaaa ttttttacag
     7921 gaatgctggt agccctgcta ctgagtttgg gaggtttttc cgtcaatgca caaacatcgc
     7981 cgactgcctc caaagaaatt tataactgga caccttacgg ggatgtcaat atgttgaatt
     8041 tattctatga tgttctgaaa cagggcagaa actatcctac accacaggaa atccgcaata
     8101 cctttggatt cgatatggaa ttttcacgtt ctcacgttcg tccgcatgcc gttatgtaca
     8161 atcaggcaaa gcagataaac ccgacaatca acccaaaacg aaaaatctgg atgaatatac
     8221 ctggtggtat cggaaagggc aatggtggtt atccatcatc actgttcaat aatgacgtgt
     8281 attccctgtg gcaatatacg catctattcg gctcttggaa tcacgggatt tttcaggctc
     8341 ccggatcctg ggcagatgct gctcataaac acggaaccga tatttttagt ggcataaaat
     8401 ttttcgaaag ctggactccg ggtagcggtg atgctactta tgcggctctg atttcgcaaa
     8461 aagagggtga caaatacaaa tatgccgaac cgcttatcaa tatcctaatg tatatgggac
     8521 tggatgggat caactataac tgggaagaca acagttatag caatactgac gtaatcgcct
     8581 ttcataaaga actttaccgg attgccaaag aaaaaggatt taataacttc cacattggaa
     8641 tttatacgct gaattccgga ctcaccgata cgaataaaga aactctgtat tatggtcaca
     8701 atccctcaga taaaacgatg gacctgatgc taaactattc agccggtaat tttgcgacaa
     8761 gcagtactgc tgcgtcgttg acagctgcca aaagtgttaa cccgaatgaa ctcagcgttt
     8821 atcagggggt gtggattgtt accatggacc gttcctggac aacgatgaat accgatacca
     8881 gaaaggaaat gaacctttgc ctttggggtg aacatgctca aaatcgcttt atgagctata
     8941 acaatggaac cgatgcattc aatatacagg aaaattacca gaaattactg gaacgtggtt
     9001 tctccggcgg aaaccgcaat ccggccaaat tgccaacgct ggctaatacc ggtaacaact
     9061 gggaattgtc gggctcaaaa gaaccgttat ccactttcgg cggtttggca agttttgtgg
     9121 ccgaacgacc cacggtgcag ggaaaactcc ctttctatac caatttccag ctcggcaatg
     9181 gagaacgata taattacaaa ggtaaaaaaa cgtttggcaa ctggtataac atgggtgccc
     9241 aggactacca gcctacctat cgctggttgg tgtacgaagc caacacaaaa acagtcagca
     9301 cagctattca gccgggctat acgcaccgcg atgcttacac cggtggttca gccctggaac
     9361 ttaaaggtaa caccacaaca agcggaacag acattgtcct ttatcgcacc gatctggaaa
     9421 tcagtgccgg caatcctcag gtaagtgtag ctgtaaaatc atacaaagag ggtaccaacc
     9481 caaccgatct ttatgttatt ctgaaaaaga aagataccga tacctggacc gaaataccag
     9541 tgggcaatac caccgataag acctggcagg aaatgacctt gcccgtgagc ggttttactg
     9601 caggtgacca catcgaatac attgggttac gtgtgaaagc ggcttcagca gctccttatc
     9661 agatgtacgt gggcaaattg gctctgacag atgacaggga agtgaaagaa gtagctccaa
     9721 tcgaagacct gattgcagaa gttaaaagag aaacacaaag taccatggcc gtcaaactca
     9781 actggaaagt aagcccgatt gccgcttcgc cgcgtgcaga tcagaccggt ctggtttaca
     9841 acgacgaagc caatgttgat cattttgaaa tcatgtataa aaatggtgtg gaaggacgta
     9901 tatcagaatt gggacgcact tctacctggt ctgcttatgc aggtgatatt gagtttagcg
     9961 acaacggtac tctggatgat ccttatattg gtgttcgttc tgcatctgtg gatggtaaaa
    10021 actattcacc ggtagtctgg ataaacattc caagagctga agcctcacaa cttccggttt
    10081 tcgccaaaga cggatattgt gctactgaga taaatccgga ttccgaaggt gctaatgtag
    10141 ctcgtgtaca acgctatctg actgaagtta aaactaccgg tttgtccgaa aacctgaact
    10201 atactgccga tgctcctgtt gctgatggta cacagtataa aaatgcaact gataaatcat
    10261 ttactgcagc tcagggacag acatttgact tcttcttcaa ggcatttgat gcgtctgacg
    10321 gtttgaaatg gtgttttgct aaagcctata tcgactggga taaaaacgga gactttgatg
    10381 ctgtgaacga aattgtattt gatttaggag cggtaagagc aggaactgtg gaatttcaga
    10441 caacaggtgt taccaaatca tttaccgtac ctgcaaatgc taatacaggg caaacccgca
    10501 tgcgtattgt attctcagac gcttggttta cacatcccgg accttgtgga caaacagcca
    10561 aaggatttag cattgatttc acggtaaata tcaccggtac aaatcctcct gtgattgctg
    10621 ttgacaaaca cgatcaggga attgccgacg aaccggaaaa agcacgcgaa agtggttctg
    10681 ctgttgaaaa tgtatatact aaccccggcg aattctcaac attttatccc aatcccgctg
    10741 aatcatttat cagcttcaag aatacgcagc agatgtggat ttacagccta gatggaaaat
    10801 tgatatttac tcagaaagat aatctggata atgtaaacat cagccagcta aataacgggt
    10861 tatatgttgt gaaaatgctg aataacagtg tggtacgaag tgccaaactt caaaagaaat
    10921 aaacgataaa ttcaactaca caaatcatgc aatatcacgc actgtttatt cagtgcgtga
    10981 tatttttttt acaatatgct atgggtgatc atgatatata cataattaat cgtaattaat
    11041 attaaatccc tacggatatt gcgttaaaat caaaataaat atatatttta cgccggtatt
    11101 ttctttatgc agctttttat tttcattttt attgtttaac tttgtggtat taaataatat
    11161 aaatatatta ataagccatc aattataaaa taatttaatt aattttgtaa cctaatataa
    11221 gagtattctg aatcagaaac aattaatttt actacaatgg aaaaaataaa aggtcttatc
    11281 aatgcccctt tcactccttt ttacgaaaat ggagaagtaa atttcgaacc gattgaggcc
    11341 tatgccaata tgctggttaa aaatggttta aaaggtgttt ttatcaacgg ctcatccggt
    11401 gaaggctata tgctgacgta tgacgaacgc attgcgttag ccgaaaaatg ggtttctgta
    11461 gctcccgagg gatttaaggt gattgttcac gtgggcagta ccgttgtaaa agacagcaat
    11521 aaacttgcac gtcatgctca ggaaatcggt gcttttggta ttggtgccat ggcttctcca
    11581 ttccccaaag tgggccgcat cgaagaactg gtgaaatact gcgaggaaat tgccagtggc
    11641 gctcctcaat taccgtttta tttttaccac atccctgcac tcagcggtgt tttcctgcct
    11701 atgcttccac tcctcgaagc tgttgatggc cgtatcccca attttgcggg catcaaatat
    11761 acctatgaaa gcatctacga atacaaccaa tgcatgctct acaaaggtgg caaattcgat
    11821 atgctccacg gacaggacga aaccattctg ccagtattgg ctatgggtgg cgcacagggc
    11881 ggcatcggcg gaacaaccaa ctacaacggt cgcgaactgg taggcatact ggatgcatgg
    11941 gaaaagggtg atttagaaac tgcacgcgaa aaacagaatt tctcacagga tgtcattaat
    12001 gtcattgtca actaccgcgg aaatattgtg ggtggaaaac gcatcatgaa actgataggc
    12061 ctcgatctgg ggaaaaaccg caccccgttc cgcaatatga gcgacgacga agaagcatcc
    12121 atgaaaaaag aactggaagc catcaacttc ttcagccgtt gcaataaatt ttagtcttta
    12181 attttgctaa aaatacctga tgataatcat actataaaaa atcacaaaac aatgagagaa
    12241 tacattgaaa aatgggcaaa aacatacaag gatgatctga ctgaaaacat tctccctttt
    12301 tggcttaaac acggtctgga tacggttaac ggaggttttt acacttgcct taaccgtgat
    12361 ggttcgctga tggacactac caaatcggtc tggtttcagg ggcggggcgg ttttgtattt
    12421 gcccacgcat ataataccat cgagcagaaa cccgaatggc tcgcagcttc gaaatcggct
    12481 gttgaatttc tggaaaaata ctgtttcgat accgatggac ggatgtattt cgaagtgaca
    12541 gccgaaggag ttccactccg caaacgccgg tatctttttt ccgaatcctt tgcggcgata
    12601 gctatgtcgg aatattccat tgcatcgggc gatcagaaat atgctcacaa agcactggat
    12661 atattcaggc ttattttgaa atataaaaat acacccggat tgctcgaacc taaatacaac
    12721 gaaaacttcc aggctcgtgg tcattcgctt tgtatgattc tgatcaatac ggcatcgtgc
    12781 attcgcaggg ctattcagga tgacattctg aaccatcaga ttgatgaatc aatcgaccat
    12841 attgccaggt atttcatgaa accggaattt gaagccctgc tcgaaatggt gggacccaac
    12901 ggagaattca ttgatacgaa tgccggaaga accatcaatc cgggacactg catcgaaaca
    12961 tcatggttta ttctcgaaga agccaaattc cgtaactggg acccaaaact ggtagaaatg
    13021 gccgaaacca tactgaactg gtcgtggaaa tggggctggg acgaagaatt cggtggcatc
    13081 atcaacttca tggactgtaa aaatctccct tcgcaggact atgcgcagga tatgaaattc
    13141 tggtggccac agaacgaaac cattattgcc actttgtatg cctaccttgc tacgaaagat
    13201 gcaaaatacc tggaaatgca taagcagatc agtgactgga cctacgctca ttttcccgat
    13261 caggaatatg gagaatggta cggttacctg caccgcgacg gatcggtagc acagcctgcc
    13321 aaaggcaatc ttttcaaagg ccctttccat gtaccgcgca tgatgatggt ggcttaccgg
    13381 ctttgtaatg aaatattgaa aagttgatgc tcaaaaggat atcaattatt attgtatcct
    13441 aattagaaaa atacaattaa aagttacctg gacataaatc ttaatctttt aacagcaata
    13501 attgcatgaa acaggcatgc caaatacttt taacttgcat gggaatgatt aaactaacta
    13561 aactatcagt acaaatctaa cttgacgctc taaatccccc ctttcccgat atcccgaagc
    13621 ttcgggacgg gacacggtag gtggttagag ggcagataaa aaattataat cgcatgaaaa
    13681 acagtaaata ttatccctgg gtagtggttg gtctgttgtg ggtagttgcc ttgctcaact
    13741 acatggatcg ccagatgctt tccaccatga aagatgccat gatgatagat attaccgaac
    13801 tgcaaaaagc agaaaatttc ggaaatctga tggctgtttt cctgatcatt tacgggttta
    13861 tgagtccgtt tggtggtatg attgccgatc gcatcaaccg taaatggctc attgtcggta
    13921 gcttatttgt atggtcggcg gtaacctacg gcatgggact ggccgaaaat ttcacccagg
    13981 tttactggct gcgtgccctg atgggattaa gcgaagcact ttatataccg gctgcactct
    14041 ctttgatagc cgattatcac accggtaaaa cccgttcgct ggctattggt attcacatga
    14101 ccgggcttta catggggcag gctgtcggtg gttttggagc aacggtagct gcagcctatt
    14161 cctggcagac cacctttcat tcctttggtc tcattgggat tacctattct gtaatcctcg
    14221 tgatctttct ttttgaaaaa agaaacgaac agaaagatat cgaaaccctg cacaatgcag
    14281 gaaaagctcc tgaaatgaag atacctttgc tcaaagggtt ggggatgata ttttcaaccg
    14341 ctgcattctg ggtaatttta ttttactttg cagcaccgag tttgccgggc tgggcaacca
    14401 aaaactggct tcccacacta ttctccgaaa gtttgaacat accgatgtca caggcaggtc
    14461 ctctttccac cattaccatt gcggcttcct cctttatcgg ggtaatattc ggtggtattc
    14521 tttccgatcg ctgggtgcag aaaaacattc gcggacgcgt ttataccggg gccattggtc
    14581 tggcgctcac cattccctca ctggtgcttt taggttttgg ttcatcactg gtggctatag
    14641 ttggcgcagc tatgctgttt ggtatcggtt acggtatgtt cgatgccaat aatatgccta
    14701 ttctctgtca gtttattcct gccaaattgc gtggcaccgc ttacggaatg atgaatatgg
    14761 tgggtgtgtt tgctggtgcc ggagtaacca gcgtgcttgg caaaatgacc gataacggca
    14821 atctgggatt tggatttgcc gtactcgcag gcattgtgtt catcgccctt gcactgcaac
    14881 tggttaccct taagccaaaa gttgaaaata tggtataagt tatggcaggt tatttgaata
    14941 ttttcattca acctgttatt ctttaatctg ttattaattt taattaaaac cgataaccct
    15001 atgaaaatta aatttgtatc attcctgctc tttttagcag caatatttac cctgagtgca
    15061 caaaacagtg tttttgttta cgaacctcaa atacccatac ttatagatcg tactgataac
    15121 gtgctgatgt atgttcgcct ggtggcaaac gatgtcaaaa cgctgaacac gattgattta
    15181 tcattcggta aagaggttaa tctgaaagaa atacgttcgg ttcgcctgtt ctattcgggt
    15241 accgaagccg atcaacgcaa agacggagtg tattatgctc cagtcgggct tatcagtgga
    15301 aattcaggaa agggacttgc agcagagcct tcatattcta ttctccgcga tgagcataaa
    15361 aatccctccg gtaatatccg ttttaatgca aatcagaacc tgtttccagg catcaactat
    15421 ttctggatca gtattcagat gaaaccaaaa acatctcttt tttcaaatat atcagctaaa
    15481 gtcacagcca ttaccgcaga caataaatcg cttgatttcg aattgaaatc ccagtcccat
    15541 acccgccgaa tgggtgtagg ggtgaggcgt ggcggcgatg acggagtagc tgcattccgc
    15601 atccccgggc tggtaaccac caatgccgga accttactgg gtgtgtatga tgcacgctac
    15661 aactccagtg tcgatcttca ggaacacgtt gatatcggtt taagccgaag taccaacggt
    15721 ggaaaaacct gggaaaaaat gcgcatcccg atggcatttg gacagtatga aggacttccc
    15781 aaaggacaaa acggagtggg cgatccctcc atattggtcg ataaacagac cggaaccatc
    15841 tggattgtag ctgcctggac acatggtatg ggcaaccgcc gggcctggtt caactcgctg
    15901 cccggaatga ctatccacga aactgcacaa ctaatgatgg tgcgcagcga tgacgatggt
    15961 aaaacctggt cggcacccat caatgtgact tcacaggtga aagatccttc gtggcgtttc
    16021 ctcttgcagg gacccggcag aggtatcagt atgaacgacg gcacactcgt ttttcccact
    16081 caatacattg acccacaggg attgcctcat gcaggaatta tgtgcagcaa agaccacggc
    16141 aacacctgga caatcagtgc acctgcacgc gacaatacca ccgaagctca ggtggccgaa
    16201 gttacgccgg gtgtattgat gctcaatatg cgtgataacc ggggtggaag ccgggcggta
    16261 agtattactt ctgactacgg caaaacctgg acagaacatc catccaatcg cagtgctctg
    16321 caggaaccgg tgtgtatggc cagcctgata aaagtggaag caatagataa tattacaggc
    16381 aaagatttac tgcttttctc taatcccaat accaccaagg accggaagaa tatgaccatc
    16441 aaagccagtt tcgacggagg attgacctgg aaggaaagca accagctcct gctcgatgag
    16501 gattacggat ggggttacag ttgtctcacc atgattgata aggaaacggt gggaattcta
    16561 tatgaaagca gtgtggctca catgactttt caggctgtaa aactgaaaga tattatcgaa
    16621 aaataataaa tcaacaatca attaagcatc aatgaaattt caacgaaacc tgttttttct
    16681 gatggttaca cttgctgtct gtgcctgtaa gccaacacct gaaaaaccgg tcaggattta
    16741 cattaccgaa gttgaaagta ttcccggtga atttggcaat atctccaaag gtgtttctgc
    16801 ctcattcgca ggtttgattg atgaaagact gattgtagcc ggcggttgta attttcccga
    16861 tactgcggca gctgagggag gaaaaaaagt attttacaag gacattcttt tgcttgatgg
    16921 tcgaaaatgg agaaaaattg gtgatcttcc gcgcgaactg gcttatggag tctcaatcag
    16981 tttacccgat gggttgttga tgatgggtgg tagaaatact gatttctttt cttccgctgt
    17041 ttatcggcta aaaatggata aaacctccga aagtctcaca attgacagtt tgttgccatt
    17101 gcctgtaaca gctgataatt ttggaggtgc tgtattaaac gatgttattt atgtggctgg
    17161 cggtaatcag gacgggaaac catcggccga agtatggagt ctggaactga aaaatcccgt
    17221 agtgtggaaa aaatgcccta aaataccttc acggccattg gtgcaacccg ttgtcaccgc
    17281 tgcagcaggt aatatttatg tcatgggtgg ttttgatgtt gccggcgata gtctcatggc
    17341 agaagtttcg caggcagtct ggatgtatgc tccgggcacc gcgaagtgga cgaaagtagc
    17401 agattatcag actgatatca atcaatattc gctgagtggg ggagcggcag tagccttgtc
    17461 cgatgacctt atactgaccc ttggcggagt gaataaaaca gtattcgaag aggcattaaa
    17521 ccgaaacgct tatctggcaa aattccagcc aattctttcg gatacgctca caacaaggct
    17581 gaaagaagag gccgggaatt atatgtttca tccggaggaa tggtataaat ttaacccgac
    17641 gataaaggct tttgatacca aaaacaatac gtggtcgagc ctgggacagt ttcagcaaac
    17701 tgctctggcc ggttcaacag tggttgcaaa cgataaggtg atatatgtgg tcaatggtga
    17761 aataaaaccg ggaatccgca ctcctaaaat ttggaaaata agccgaaaac aaagggattg
    17821 aattgaaaca tttagtacat gaacttgttt attctttgta ttgcttataa aacaaccaaa
    17881 atataatcta tgatttattc aactttaacc gagagcaaac acatcgaaag tttgcatccg
    17941 aaattcaaac aacttttcga ttatgtgaag gagcatgact tactacgtca ggaaacggga
    18001 cgcataaccc tcgacggcga taatctgttc atcaataatg ttaatcccca aatggttagt
    18061 gctgatgagc aggtactgga agttcaccag cgatatctcg atgtacatat tccgcttgat
    18121 aaagctgaaa ctatcggcat caaaccgctt gaaaactgca ccgatctgaa atcggaattt
    18181 aattctgagg atgattacgc tctttattcc gataaaccta caaattatgt aactttgcaa
    18241 cccggtgaat ttctgatcgt atacccgcac gatgcacatg cacccatcat tggtgaagga
    18301 aaaatcagaa aactgatagc caaagtgttg atttaattca ttataataaa aaaattattt
    18361 aataaaaaaa tactatgata tttgatttaa gttcaaaaat taccctcgaa agaataccca
    18421 agaagtattt tcgtccctca gacccacttg aagcaacccg ccagtcgagg tacgagaaac
    18481 tccgtaccga catctacgag actgaaaagg aaggtgcaca gtctgtagca aaagagatag
    18541 ctgctcttat ccgcaccagg gcagaacaaa acaaaccctg tgtactggct ttgcccggtg
    18601 gctcatcacc cctgaaagta tatgccgaac tggtgcaaat gcataaaaaa gagggcttat
    18661 ctttccaaaa tgtttacatt ttcaacattt ccgattttta tcccgttgac agtaaacatc
    18721 attccaatgc ccgactgata aacgaatatc tcctgagtaa agtggatttg cctgaagatc
    18781 atttttttac accggccaac attgaacgaa cagatgtatt gaatttctgc caacgctacg
    18841 aagccaaaat ggaagaattg ggcggacttg attttgtgtt ggctacaatc ggtcaggtag
    18901 gcaatctggc ttataatgaa ccgggttcgc agacaagctc cattacccgt ttgatgttgt
    18961 tggattcgca atcaagatca gatttgaagc aattttacat atcgtccgat gaagtacctg
    19021 taagcgctgt tacgttagga atttccacaa ttttatcagc taaaagagtg gttttgatag
    19081 cctggggtga aacaaaatcg aatattataa aagaagttat tgaaggcaaa gccaatgaca
    19141 atgttccggc ttcattcctg caactgcaca aaaatgcaca agccgttctt gacctcactg
    19201 ccggtcagca tcttacccgt atcagtcatc catggctggt aggaccctgc gaatggacgg
    19261 acaaactcat ccgccgcgcc attgtgtggt tgtgcgaaaa aacacaaaaa ccgattttga
    19321 aacttaccaa caaagattat aatcaatacg gattgaacga attattggcg cttttcggat
    19381 cggcatataa cgtaaatata aagatattca atgatttaca gcgtacaata accggttggc
    19441 cgggtggaaa gccaaatgcc gatgacagca accgtcccga acgggcaact ccctatccaa
    19501 aacgtgtact tattttcagt cctcatcccg atgacgacgt gatctccatg ggcggaactt
    19561 ttcagcggct ggtggaacaa gatcatattg ttcacgtagc ttatcagact tccggcaata
    19621 tagccgtagg cgatgaagag gtggtgcgct atctgtcact catgagcaat attctggagc
    19681 atttcgaccc tgaaaatgat aaactaaaaa ccagatatga agatttgcgc catctgcttg
    19741 ttaatgaaaa gaaatcgggc gatatggata atccggaaat tctttatctc aaagggagaa
    19801 ttcgtcgtga agaagctcgt actgcctgtc attacaacgg tgttccactc gaacgggtac
    19861 attttctgga tcttcctttc tatgaaacgg gaaagattaa aaaaggtgat ctcaccgaga
    19921 aggatgtgcg tatagtgatg gatttgttac aggaattgaa acctcaccag atattcgttg
    19981 ccggtgacct tgccgatccg catggtactc accgggtttg tctcaatgcc gcactggccg
    20041 ccattcatga gttgaaagac gaagaatgga tgaaagattg ccggatatgg atgtatcggg
    20101 gagcctgggc cgaatgggaa atagaccaca tcgaaatggc tgtgccaatt agtcccgaag
    20161 aacttcgtcg gaaacgtaat tccatactta agcaccagtc acaaatggaa agtgcaccat
    20221 tcctgggtaa cgacgaccga ctgttctggc aacgggccga agaacgcaac caggctacag
    20281 cgaaaatgta taccaatctg ggacttgcat cctatgaagc tatcgaagct tttgtgcaat
    20341 ataccatatt ataaatttat tggaaataga ataataaacg ctcagttcta accggattgg
    20401 gcgtttattg cataaaatat ttgtagtttt gtaattgtat agaatgtata acaaagagtg
    20461 ttaatattat tttaatgtaa acccaaacaa tacaattcca ttatgaacct actcgaacaa
    20521 cttaaaacct tcacccaggt ggtagccgat accggcgatt ttcattctat cgaagtgtat
    20581 aaaccggtgg atgccaccac caatccatcg ctaatatttg cagcatcgca ggacccaaaa
    20641 tacaaggaac tgattgatga agctattgtg tatgcacata accaatcgcc ggtgaaatca
    20701 attcagctca gcaaggcaat ggataaagtg gccgtgaact tcggacttaa aattctcgat
    20761 attgtacccg gacgggtttc aactgaggta gatgcccgct tatcgttcga tacggaggca
    20821 acagtaatga aaggccgtga attaattgcc ttgtacgaag cagccggagt ttcgcgcgaa
    20881 cgcgtgctga taaaagtggc ttcgacctgg gagggaataa aagcagcaga agtcctcgaa
    20941 aaagagggaa ttcactgtaa cctcacgttg ttgttttcgt ttgtacaggc ggtagcttgc
    21001 gccgaagcag gtgtgcggct catttcacca ttcgtgggac gaatactcga ctggtacaaa
    21061 aagagcaggg gagttgatgc cattcctgcc aacgaagatc cgggcgtatt gtcggtgaaa
    21121 aatatttata attattacaa gaaattcggg tacaataccc aggtgatggg agccagcttc
    21181 cgtaataccg gtgaaataat cgaactggca ggatgtgatt tgctcaccat atcgcctgca
    21241 ctgcttcatg aactggaaac ttcgtcagga aaactggaat gcaaactgaa tgccggagat
    21301 gccgcaaagt ctgctgttga aagaatttcg gcggatgaaa aaacgtttcg atggatgatg
    21361 aatgaggatg cgatggctac cgaaaaactg gccgaaggta tccggaactt tacaaaagat
    21421 ttgattaaac tcgaaaacca gattgccggt ttattgtaag ttgagactgg attaaaaata
    21481 aaaagtcttc atttcgtatt gagatgaaga ctttttttat tttcactgac ctattagctg
    21541 ccaaaatcgt cgaacattag attttcacgc ggtactccca aattccagag catatcttcc
    21601 actgctttag ccattgggcc gggaccgcac atgtagtatt caatatcttc aggtgcgtca
    21661 tggtcattca gatacgtgtt ggcaagcacc tgatgaacaa atccggctgt atatttcact
    21721 ccggcttcat cagctacggg atcggcacgg tcaagtgcca ggtgaaacga gaagttagga
    21781 aattcttttt ccaatgccag aaaatcttcc atgtagaaaa cttcattaag agcgcgggct
    21841 ccataataat acgaaatttt acgatcccta attttcaacg tctttaacag gtgaagtatg
    21901 tgtgagcgca acggagccat accggcacct ccacctacgt agatcatttc tgccttgctg
    21961 tcatacaccg gatggaattc gccgaacgga ccggaaacca tcactttatc gcccggtttg
    22021 aggttgaaaa tgtaagagga ggcaataccc ggacttactt tcatccagcc accatttacg
    22081 cgatcgaaag gaggtgtggc gatacgaacg ttgagcatca caatatcacc ttctgccgga
    22141 tagttggcca tggagtaagc acgcatggtc tcctcgttgt tggtgcagct cagatcccac
    22201 atgttgaatt tatcccagtc tccgcggaaa cgctcatcca catcgaattc actgtatttc
    22261 aggctgtatg ctggtatatc tatctgaatg tagcttccgg gaataaaatt gagatgttcg
    22321 ccgttcggaa gtttcactac gaattctttg ataaacgtag ccacattgtt attggatacc
    22381 acttcacatt cccatttttt tactcccaga attgattccg gcattttgat ggaaatgtcg
    22441 tttttgactt taatctgaca acccaggcgc cagttgtcct taatttccct gcgggtgaaa
    22501 tgcacagttt cggtaggcag aatttcgccg cccccttcga tcacctggca ccggcactga
    22561 gcacaggatc cgccgcctcc gcatgccgaa ggaagaaata tactctgggt ggataaggtg
    22621 ttgagaagcg tacttccggc ctgggctatt acttcttttt ctccgttgat aagaattttt
    22681 acatcaccag aagttaccag gtatcttttg gctacaataa gtactacaac caaaagcagg
    22741 ataaccacaa ggaaaacact taaactggca actatggccg ctgtattaat ggataatatc
    22801 atcttgaaat tataatttat tcatttacat tttttttgtt gggcaaaatc agattttaag
    22861 acctgaaaat gccagaaagg ctattgccat cagagctacg gtaataaacg tgatacccag
    22921 accgcgcaaa ggtttcggaa catcggaata ttccattttt tcgcgaatgg ccgccagccc
    22981 cacaatggca agcaaccaac ccactccgga gccaagtgca tacgaagtag cttcgccgat
    23041 attgttgaat gcacgctgct gcataaagag tgaaccgccc atgatggcgc agtttactgc
    23101 tataagcggc aggaaaatac ccagggaggc ataaagcgac ggggtgaatt tctcaactac
    23161 catttccaca agttgagtca tggctgcgat cacagctata aacaggataa aggccagata
    23221 gctcaggtcc agcgaagcaa ggccggggct tatccatgcc aatgcacctt ctttgagtac
    23281 atagttttcc agcaggtaat tgaccggaag ggtgatggtc aatacgaaaa tcactgccac
    23341 acccaagccg agcgaagttt ttacattctt agaaacggcc aggtaagagc acattcccaa
    23401 aaagtaagcg aaaatcatat tgtcaacaaa tattgacttt acaaatatat tggctatatt
    23461 ttccatatca taattttatt gagtagtgtg aaagatgttc tgaatatcag cggatcacac
    23521 cggtttgatt atttttcctg taagtcttta tttttcgaac gatgaaccca aatgacgcac
    23581 gcaactataa tcaatgccat gggaggcatc agcataaggc cgttgttcat atatccggcg
    23641 tcgtacacag cctgtggaat aacccggatg ccgtaaagcg aacccgaacc gagtaattct
    23701 ctgaaaaagg caacgatgat cagcacaatt gcatagccga atccatttcc aagtccgtcg
    23761 acgaacgatt cccagggttt gttgctcatg gcgaaagctt ccaggcgtcc cattagaatg
    23821 caattggtaa tgataagacc gatatacacc gatagctgaa cacttacatc gtaggcaaat
    23881 gctttgagaa attcgctcac aaccgttacc agtgctgcta ctactaccaa ttgaacgata
    23941 attcggatac gattgggtat tgtattgcgt agcaaggaaa taataacatt cgaaaaagcc
    24001 acaataatgg ttaccgagag tcccatcaca atggcaggct ccagtttggc tgttacagcc
    24061 agcgccgaac agatacccag tacctgaacg ccaatcggat tattcttgct cagaggtccc
    24121 agaaaaacct cttttgtttt agctgaaaat agtttactca ttgttttgct cctcctgtag
    24181 ctggttgttg taaaaatgtt tcgtactgcc cgatgcaatt catcaacatg gcatccactc
    24241 ccttactggt aatggtgcca cccgatattc catctacata gtccattcct tcggcggttt
    24301 gtccgggttt taccacggca atagagacaa atttattttc gttgttcatg actttctttc
    24361 cgataaattc tgcctgaaaa gatttttgtg aaatttcggc acccaaaccc ggcgtttcgc
    24421 tggcatgtga aaaatatgac ccaaaaacgg tgtttttatc ttcattcagt gacagataac
    24481 cccatattgg cccccataga ccggctcctc gtagcgaaac aacgtatttt gttttaccat
    24541 caatgtttgc tatgtacact ggtaattgac gtttttcaag cggtttgctg agttccttgt
    24601 ttatatcaat ttcaaaagcc tgctctttcg aatccgaaag cacttcagct ttggaattaa
    24661 tgacaagacc gccggttatt attttgttaa aaagttcgtc ggcatcctga ttatcaattt
    24721 ccacactcag tgaagtcagt atttgttttt tcttgtcgag tttcacattc gctgtctgtt
    24781 tgtccttcag tgctcccgac acgaaagcaa gcataagtgc cactatcaca accattacgg
    24841 ttgcaaaaat tattgtatat gaatttttat ttgtatccat cttcctcgtt gtttatttat
    24901 taacagctct tttcaggcgg cgttttacat ttgcatccac tacatagtag tctataagtg
    24961 gtgcaaatat gttcatcagc agaattgcca gcatcatacc ttcagcgtaa ccgggattca
    25021 gcacacggat gacaatagcc attaccccaa tcagaaaacc atatatccat ttaccggttt
    25081 cggtacgcga tgaagtgacc ggatcggtag ccataaatac agctccgaat gcaaaaccac
    25141 ccaatacgag gtgataatac cagggcaaat tggctgctgc ggtatccgga ccaaagtgat
    25201 taaatataag tgccataagt gtgccaccgg caaataccga aagcattgtt ttccagctgg
    25261 caacacctgt gagaagcagc aaaaacgccc ctatggcaat ggcaataact gaagtttcgc
    25321 cgatggaacc cggaatgaaa ccggtaacgg catccatgaa ggaaaactga tggccgaaca
    25381 tatcggtaat ttgtatggtg ggtgaggccg atgtggctat ctgaccaagc ggagtggctc
    25441 ccgaaaaacc atcgaccacc tgaccgcctc cgaacccaaa cgtgtcctgc gttcgtatcc
    25501 agactttgtc gcccgacata gccgacggat aggcaaagaa aagaaatgca cgcgtgatga
    25561 gtgccacatt gaaaatattc atcccagttc cgccaaaaac ctcttttgca aaaattacag
    25621 caaatgcagt cgctacggct atcatccaca gcggcgtatc aaccggaaca atgagtggaa
    25681 ttaaaaatcc cgtaaccaga aagccttcct gtatctcatg acctttgatc tgtgccacta
    25741 cgaactcaat acccagccca acgatgtacg aaacgagtac gataggcagt accgatataa
    25801 atccaaacca gaatgtagcc caaaagccgg catcctgacc gatggccata aagtgctgat
    25861 aacctacgtt gtacataccg aaaagcaatg ccggaacaag tgccaggacc accatgatca
    25921 tggttcgctt ggaatctacg gcatcgtgaa tgtgagttcc ccgttttgaa gttgtattcg
    25981 gtacgaataa aaaagtctca aaaccatcga aaacggagtg aagcttttca aacttacctc
    26041 ctttttcaaa gtcgggttta atcttatcta ctaaatttct taatttgttc atattttgtc
    26101 atacttgttt agataattgc atgccggaag tactctgaaa attagcattg tactgaccga
    26161 tagtcaaata gctgtagaat atcccgttaa gtgttctgta atatttattt tgaagaagat
    26221 tacaatatca aatctattgt tattccactt ccgatttcaa aaaatcgagt gaatacctga
    26281 caatggcttg aagtggcagt tttgatgtgt ccacaaattc gcaaagcgca aaatcttcgg
    26341 gtgcaacttc atacgctccc aggttttcca ttttgtcgat gttttttgca atcattgctt
    26401 ttatcagatg ttccgggaga atgtccatcg ggaatacttt atcgtattca cccgacatga
    26461 tgatggctcg tcggccaccc ataagccggg catcgtattt gaattctttt tttctcagta
    26521 atttagtgag gtacaaccgg ctggcactga atttgttgaa acggggcata gcccatccga
    26581 gcatttcatg cgtttccgtg ccttcgtcga taaccgatat ttgtgagcag cagggagcta
    26641 aaaatccatt ttcggtgatt tttctaccta caagtacatt gccgctaatg aatcgcagcg
    26701 gataacttac ttttttcaaa tttcctttaa ttatttcaga aatattattt ccggaaatgg
    26761 tttcgaaata ttggggattt tcaacttccg gacctgtgag tgctaccagg cgtgtgaggt
    26821 ctacaattcc tttattgaac aggcgaccga tgaacagcac atcctgcgga tggatagtcc
    26881 agacaacttc ccccttgttc accggattga tgtgattaat atgtacacct acgttgccgg
    26941 ccggatgcgg accgctgaaa acggttgcta tgacaccttt cacagtgctg aaaacagaag
    27001 catcggactt cagtcccaaa tgaacgttgc cgctggtaag ttttgaaagt gcatctacac
    27061 ctgtctgaaa atcagcaagt tgatccctga tgataaattc ataatcgggt gccaggggag
    27121 cggtgtcaaa agtggagatg aatattgctt tcggactctt agccggattg ggaatgatgt
    27181 cgtacgggcg ctggcgcata aacgaccaca atccgctttc cagcatcaac gccctgattt
    27241 catcagcagt actatccgac ggttttttga ccgaaaactt cttgtattca atcgttttat
    27301 cggtgtcaat agtaatgtgc atcacttttc ggcgttcacc acgtacaatt tctctcactt
    27361 ttccgcttac cggcgataca aagttcattt ccggaaaact tttttcatga aataaaggtg
    27421 tacccgcatt cacagtatca ccttctttga caagcacttt gggggtgatg ccatgatatg
    27481 attccggatt gacttcgaaa acagcggaat gtggaatttt tgatattttt tcagcagctt
    27541 tgccgctaat ttttatatct aatcctttgg ttatttttat tgtatttgcc atcaaaaatg
    27601 tgatattgaa tttttactaa aaacgacaca aagatacttt ataaatctta attacggaaa
    27661 aatccatttc tcaattaact aaaaaatcct tattattttc aaagaactgg cataatattc
    27721 ctgcgaatga ctctacctga aatgaagatg aaaaagtgct ttgtagttca gttttaaaat
    27781 taaagtaaag tttgatggat gaaaataaaa ttattgcttt atttgtacaa ttaaaactac
    27841 cggtatgata cgtgatatta ccatcgcaga tgctgctcag atttgcagta tatacaacta
    27901 ttttattgag aatacagcca taaccttcga actgacaccg gtagaagagc ctgaaatggc
    27961 aagacgtata accgaaatta cgaagaatta tccctggatt gtatatgaag aaaacggtga
    28021 aataatcggg tatgcttatg cctccaaatg gcgcgaccgg ccggcttacc gatttgtagc
    28081 tgaaacgact gtatatatcc gccaagggga tgaaggtaaa ggaattggaa gcctgttata
    28141 cactgatttg ctccgaaagt tgaaaatgaa gcatattcat gcggcaatcg gatgtatcac
    28201 cctgccaaac ccggcgagtg taacgctgca tgagaaactc ggttttgcca acgtagcgca
    28261 tttcagggaa gttgggttta agtttgacca atggcaggat gtgggcttct ggcagctgat
    28321 gttctgatat ttaataaaaa ttacaacaaa gcctgaaaat tcattataaa aataaactgc
    28381 ctccttttca aaaaaagcag gcagtttcaa tattgtttta tttgagcaga tatttgcttt
    28441 ttacagttat tgtggctgtt ttgagcttcg atgaagtatt gtacgaacca ccccagctgt
    28501 aatcttccga tgagttttga gcaataatct ggaagacacc catgtttgaa tccaccatgg
    28561 ttccaagctt accttttccg ttttttgcca tcgtttctgc ccgattgtag gcatctttgg
    28621 ctgcttcagc aagcatttct attttcagat ccgatagttt ggtgtaataa tattcgggag
    28681 ccagcgatgt aatttctatt ccctgattga tgagttcggt gatttccctc gaaatggttt
    28741 caatctttga aacgtttttc gaatttattt taacgctttg tgtcaacctg aaaccatcaa
    28801 aattggagga tgcaatgttg ccgttcgggc cgtaggtata actgaaatcg cggttgattt
    28861 ccacggccga gaaggtgatt tcgtctttgc tgactccttt ggataacaga aactgacggg
    28921 tagtctcact ttgttttttg agctcggcat atgcttcggt caggttgaat ttctttactg
    28981 taaactgact ttgccagaca atcaggtctg attcgaaatt gcgctcggcc atgccggtta
    29041 ccattattgc ctgatttcgg atgttacggt taattacaga gcgcccgatt accgcactgg
    29101 caatgatcag tgctacagaa agtagggtca tgacaataat tcgttctgat ttcattttag
    29161 tgtgtttttt atggttagtt gtaatgtttt agtttctgta tttattgatt tatttttatt
    29221 ttatcaccta ttcgcaggtt ttcgtccagg tttctgttcc aatccttgat ttcagcaact
    29281 ttacatccgt attgtctgga cagggagtat aatgtatcgc ctttttttac cagatgaata
    29341 atggttcccg attttataac tgccggtttt tcggattttt tttcgggagt tactgaagtt
    29401 actgaagttg catcagaagc cgtagatttg atttcggagg tagatggaac aagtacgatt
    29461 tcggcagatt gaggaaccat aactgctagt aactgaccgg gtgtaatatt gttcgatttc
    29521 aggttattca ttgatttcag tttttcaaca gtcaggttgt gagtacgggc gatgccaaac
    29581 agcgattctc cggctttcac cttgtaaagt cctgtaccct gttgtgattt atccagggtg
    29641 atgcctgcga taggtttttg ctcgtctctg agtgtgatgg cgtctttgga tacctgattt
    29701 aatccggttt ttttgatttc aagcgatggg gtaggcgaca tggaagcata atggagtata
    29761 acctttgaag gttcggcttc ctccactttc tgcgacccgg ccacctgcaa attatccagt
    29821 gtaggttcgg tcgatgcaag taccgcctca ctgattatca gttttttgcc acgttttaat
    29881 ttgctgtttt tcagtccgtt gagatacttt agcgtgctta ttggcacgtc gtaatcctgc
    29941 gcaatcgacg aaagtgtctc tcctttttta actttatggt attttgcatc aaccagtatt
    30001 tgttttttgt ttttgggaat caattcgaat ttagactttt tcttttctcc cgaataaagt
    30061 gtaattgtcg aatcgcgatg aacaaacgta ttatctgcca ggagtgctct cccgcctttt
    30121 acaaaacggg aaccataata ttgttcttcg gaggaggaaa tggtaacccc tcttttgatg
    30181 gatgaatgta tgaaattaaa ttctccgttt ggcttagttt cagttacaat gcccacgtgg
    30241 ccgacgattc cgttatgtcg gcgtccttca aaaaaaacca ggtcgccggt ttgcaaatcc
    30301 tgtttgttga tggtgggaag ttgcgaggcc tgatcgcggg aagtacggtt caggttgata
    30361 ccaaaattat tgaaaacgtg cgaagtaaat cccgaacagt cgaaaccgcg cggtgtagta
    30421 ccaccatagc ggtagggtaa cgacaaatat atttttccaa agttgatgac agaatctttc
    30481 agcgtgattt tgcttaaatc cggaagcata accgtatcgt taccttgatt ttcggtaaca
    30541 acaggttttt tataaaaagc aaaggaaggt aatgataaac tgatgaatag tataattgtc
    30601 agggctattt tcattctata attgtatcag attataaatt tggcaatacg aatgatgttt
    30661 caaactttac aaaaatactt tttataattc agaataatga ttgctgtttt tctttttaaa
    30721 caaaattaga atccatttag atttccggaa aagtggagag ttcttggagt aaatcttcaa
    30781 ttgtcagaag gattttaccg ttgatttccc agcccttttc ggaaatttta atatgtgttt
    30841 cagcatcagc agtaacactg taaccggcac gtgccagtgc ccgcagggtc cagtaaagcc
    30901 gtgttttgtc gccgctaacg ctaagttgtt tgtttaacgg ataattcatc gaaaaattgc
    30961 cgttggcagc attgaagtag taggcttcac tctgaaaaac ccggttgaaa ctgccgtcga
    31021 taaccagatc ttcgggtacg ccgcagatta caccttctgt actgctcatc agccagatgc
    31081 ggtcggcagc ctgcagtgcg aggtccagtt cgtgggtcga aagtatgatg cctttgcctg
    31141 ttttgtgtgc caggcggtga agcaacaaca tgatttccac tcggttggga agatccagat
    31201 gcgctgtggg ttcatcaagg aaaataagcg gtgtgtcctg tgccagtgct ttggctatca
    31261 tcacccgctg ccgttcgccg tccgaaagtt cgttgaggta atgattggtt ttatgctcca
    31321 gatgcaccat acgaatggct tcgtgcacct tttcgcgtcc ttcgggtgca agtccgccaa
    31381 gccagtgggt gtagggatgc tgaccgaggg taacaatatc gcgtaccgtg gcattttcaa
    31441 tctgcacttt atcggtcagc accagcgcta tttgcagggc tttttcctgc tgcgtgaatt
    31501 ccgtaatgtt tttttcttga attaaaacat tgcctttcaa cggtttttgc aaaccggcca
    31561 gcgtacgcag caacgtggtt tttccactgc cgttgggtcc gatcagacac accatttcgc
    31621 cccggagcag ttcgaggttc aaattgctct gcaccaacgt tttattgctg cctttggcgt
    31681 atccgatgga aagctgactg gtttttacga tcgacatatt tattttcttt tcaaaataat
    31741 ccagataatt accggtgctc caaataaggc actgatagta ttgatgggta atgtatgcgt
    31801 cggaatttgt gaaataatat cgcagattaa aatcagcgaa gcacccagca agacgcaggc
    31861 aggcatcagg agctgatgtt tcgctgtgcg aaaaatgccg cgggcaatat gtggtatggc
    31921 tactcccaca aacgctatcg ggccggtgaa agccgtaatg gcgccagcta aaattccggt
    31981 ggctgtaact atcagcaccc gggtttgaag aatgggaatt ccaagtcccc gggcataatt
    32041 ttcgcccagc aataagccgt tcagtctttt ggtaagtatg attgccagca ataaacctgt
    32101 aagtatcgtc gggaccagga tttgcaaata ctcccaggtt acggcactga ggctgcccat
    32161 attccacata atgaaaagct tgatggcatc gggattgctg aaatattgca gcacacccac
    32221 cagcgaggag gcaatggtgc cgaacataat accgactatg agcagtgaaa ccgagttctg
    32281 aaccttaaac gaaacgccta cgaccagcaa aagcaccagt gcagctccga taacggctgc
    32341 cagtatcatt ccccagccgc tggaaatgaa tacggcgggc aaaactgccg atgccatcat
    32401 caccagtgct acacccagac tggcacccga acttacgccc aaaatgtacg gttcagccag
    32461 cggattgcgg aaaagggtct gcatcatcag tcccgctacg gaaagtgaag cccctgcaag
    32521 gatagctgtg atggctttgg gaagacgaaa attgagcaga atttcggtat taatcgtatc
    32581 ggaacttgcg ccgaaaagaa cattcattgc ttcgccgaaa ggaatacgga tgcttccaaa
    32641 gctgaggtcg gccacaaaaa gcactgccag cagcactgta agcaaaatga acagtatcgt
    32701 tttgcggggt ttcatccggt ttatttccat tcttttttaa gcagactaaa tacttcaaga
    32761 tcaacaaaat gaccatccgg aaaaagttca ccatcgcgtt ctataccttc aaatataaat
    32821 cccaaccgca tcgggatatt tttgctgggt tgattgccca ctgcacattt tatcgctatc
    32881 cggttaagtt ccagttcatc aaaggcataa tctatgagca tccgtgcaga ttgagttact
    32941 attccttttt tctgaaaggg ctccgacaac cagtaaccga tttcagtacg tttgttggct
    33001 ttgtctgccg gattaaattt caaaccgata agaccggcaa attcgttccg gtaatggatg
    33061 caaaaaacca actcccgaaa aaactcgggt gtttcagtca ctgaccagat aaaagccatc
    33121 gtatcgagta tcgtttttgt gccgggcaca aaaggaagcc accgacccag atactcccgc
    33181 tggctgtcga taacccggaa gatgtaaggt gcatctgttt cttccagttg tttgagcgtt
    33241 atttcatccg aaacgattag ttttatcata atttcacttg aattggatgg gtttatttca
    33301 atttttcagc gtaaacgaat tcataatccg gtaaaagttc aggatgcaga atggcaatca
    33361 catcgcccaa cagcaaatcg ggccgtgcta cggcactttc ccagaagtcg ttggcagtgg
    33421 agggaagcag
//
LOCUS       DAOPZK010000003        33186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1236_length_33186_cov_23.647732, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000003 DAOPZK010000000
VERSION     DAOPZK010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 33186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33186
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_33186_cov_23.647732"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..2827)
                     /locus_tag="PKH68_00310"
     CDS             complement(137..2827)
                     /locus_tag="PKH68_00310"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNX11407.1"
                     /translation="MKIHFKLSYFTKWGQRILVSGNIPELGNDDFSRALPLRFQGHED
                     WVAELDCPVTENQVISYKYLLYDETNSSYTEEWGDDRNVAVYSNNPEHYFCFDTWNSV
                     SSNDNVFMTSPFQEVLLKVNTATAPPKRNKKFTHIFKVKAPLLHARQTLCIIGNCKEL
                     GSWSVTEPLMMYKEGENWIVKVDLSKVKSEVHYKYGIYDFDEQHFMFFEQGPDRIAAV
                     IGSANTLVQVHDGFVRMNNNSWRGTGVSLPVFSIRTRRSFGVGDFEDMKLFIDWAVKV
                     NMKLIQVLPLNDSNGTHTDADVLPYAAISAFALNPLFLHLPALGKLPETNSLAQQYIP
                     KQAELNALPLVSFLEVINFKLQYAREAYLYQKEEFLKDADFLAFFDKNAHWLVPYAAY
                     CVMRDKFGTNDYRQWNEYAVYNAELIAAFTAKTQPHFDEVGVNYFIQYHLHKQLSEAA
                     RYAHENGVVLKGDIPIGINKNSVDTWVSPELFHMDMNAGAPPDMFAIKGQNWELPTYD
                     WEAMKRTGFGWWKKRFEQMSNYFDTFRIDHILGFFRIWEIPTNQLEGIMGHLNPSTPI
                     YINEFGEKGIWFDYNRFCKPYITDQILWDFFGEESAWVKANCLQIEDGWVLRLKPEFQ
                     SQTYVDELFNEGKISERVKWGLFDLISNVLLFEVKGSNGQQFYPRYGMHSLRSYIELD
                     DHTKKGLDELYIHYFYHRQDENWYHSGMEKLPALKRSTNMLICGEDLGMMTPCVTAVM
                     KELGILSLEVQRAPKSNKIEFFHPNDAPYLSVVTPSTHDMSTVRGWWEEDRSVTQKFF
                     NTQLGHWGEAPYFCEWWISRDIILQHLYSPAMWAIFQMQDLLGISPKLRRENPHEERI
                     NIPSNSMSSWRYRLHLNMEDLLQEDEFNNELRNFIVQSGR"
     gene            3191..4135
                     /locus_tag="PKH68_00315"
     CDS             3191..4135
                     /locus_tag="PKH68_00315"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNX11408.1"
                     /translation="MSEDSTLFKVFSGTKSRYLAEKICKSLNWPLGKMNIEHFSDGEF
                     SVSYEESIRGQYIYLVQSTFPNSDNLMELLLMIDAAKRASAYKVIAVIPYFGWARQDR
                     KDKPRVSIGAKLIADMLTVAGVDRVITMDLHADQIQGFFNVPVDHLYGSTIFIPYIKS
                     LNLENLVIASPDVGGSKRAGSYSKHLEVPMVLCHKTREKANVVGEMRIIGDVVGKNVV
                     IVDDMVDTAGTLCKAANLMIENGAVSVRAIVSHGVMSGSAIKNITNSALTEIAFTDSI
                     PFDCNKCEKAKILTISDMFADTIRRVQEHKSISEQYLL"
     gene            4293..4721
                     /locus_tag="PKH68_00320"
     CDS             4293..4721
                     /locus_tag="PKH68_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11409.1"
                     /translation="MKNLAALFVVLLSVMFTLPQQLQATPPDWAPANGYRDQTRHIYF
                     PQQNIYYDLQKEVYMYDDGGLWFVIKSVPEKYKNINLRKSPQVQLFLNTDAPYTENET
                     HRTKYKYLVHESEKTIRETEKLIRKEQKAQEKAERKSEKK"
     gene            4995..7454
                     /locus_tag="PKH68_00325"
     CDS             4995..7454
                     /locus_tag="PKH68_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX11410.1"
                     /translation="MHTRKISVLFLIFISLTLSAYAQRYTISGYITDQSNGETLLNAS
                     VFDDVSRKGAVSNNYGHYSITLNAGDVSLNYSYVGYSPQFREFKLTKDTVIHVKLEAS
                     ITLREVTVTGRRSEVGVHSTQMSAIEVPIEQIKNIPSLFGENDLIKALQLLPGVQSGT
                     EGSAGLYVRGGGPDQNLMLLDGIPIYNVNHMFGFFSAFNTDAVKNVTLYKGSFPARFG
                     GRLSSVVDVRMKDGDDKNYHGNVSVGLISSKFNLEGPIWKEKTTFNISARRTYIDVLS
                     QPIIRYYSRQNMGEDAGSTTAGYYFYDLNAKFTHKFSEKDKLFLSAYLGDDAIYTKFR
                     TDKTSYGERSENDWMNLNWDWGNAIAALRWNHVVTNKLFMNITGAYTRYRSNMNVGYL
                     SESTDKTTNPPKTQTEEFKVGYRSGIEDYSLKADFDYSPNTSHDIKFGTNYTYHTFRP
                     DVSTFYFFNNNNSQNQKIDTVIGKGNIYAHESMTYVEDNITLTDRFKINAGIHFSTFN
                     VRQKNYYSLEPRLGFRALLTDYLSLKAGYAHMNQYIHLLSNNSISMPTDLWVPATQLI
                     DPMKSDQYAFGIFYNLKNLFDLSLEGYYKSMDNLIEYKDGASFMGESSGWEEKVVMGR
                     GWSYGVEFLAQRSFGNTTGWLGYTWSKTERLFDRPGQELNYSRIFPAKYDRRHDLSLT
                     VMHKFSEKIDLSGNWVFATGNTATLGLHTNEGPVIPNVNEAYNYPAPQTYISSRNNYR
                     YNNYHRMDLGVNFHKKKKHGIRTWNISIYNVYNQFNPFMVYQSSEGSYDARTGRYESK
                     NVLKQVTLFPIIPSVSYSYKF"
     gene            7459..8460
                     /locus_tag="PKH68_00330"
     CDS             7459..8460
                     /locus_tag="PKH68_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNX11411.1"
                     /translation="MKTKHILAVLIIGLLTCGCDKTIEFNGKITSPMLVVSSFVTPDS
                     VIKAEVTKSRFFLDEGYVFERVTNADFNLYVNGILKEKMVHTGYGIYLSGYRPHPGEE
                     IKIEVAAQGFTTISGTSVIPAQTPILKVDTSSVIEKEYYIGSKGGYGGMNSQDTLSEL
                     TLKKISFRVKFKDDGDKRNYYRLVVRKRIYYEDSALDVYLGNFEDIVFGNQQTNDIGS
                     LFEFSRNNYNYDTFADDLLNGKEYELKFYNIITVDQKDFSGYESQFRKELRSTYFIYL
                     QQISPDYYLYARSSSAAANLSGNPFTEPVQIHSNIKNGIGVLGSYTSSAAAVVDVVN"
     gene            8620..10416
                     /locus_tag="PKH68_00335"
     CDS             8620..10416
                     /locus_tag="PKH68_00335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNX11412.1"
                     /translation="MESFLVSARKYRPATFRSVVGQESLTTTLRNAIKSNKLAHAYLF
                     CGPRGVGKTTCARIFAKTINCEHLTEDYEACNQCESCKTFNEQRSYNIHELDAASNNG
                     VDDIRSLTDQVRVPPQVGRYSVYIIDEVHMLSQGAFNAFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQIYDFNRISITDTVNHLKYVAGEEHVTIEESALNVIAQKSDGGMRD
                     ALSIFDQLVSFSGNNITYQDVIDNLNVLDYEYYFRLVNAFLHGNVAESLLLFNEVLNK
                     GFEAQHFITGLSSHLRDVLVSKDATTLQLLEVGAEIAERYRQQASACPTEFIFDALKI
                     SNDCDLNYRISKNKRLLVELALIRISQLTTDKKKVLTAEKAPLKPLEAAETVTTKQPG
                     QVGSQTASAATPPQQIQPPAESAPAEITPTAATSANPVEVTVAPRKPAEVSVGKINRP
                     PSISISSIKTEPTGISKPDIVSDTEGVFATDKPFTAAELTDAWKLFAGRFEKDQVTRG
                     FLLSHIPTIGMDNNINLSLDNKFQEKEISQMHALEFLRKTLHNNSLNFQIDIIPDEEQ
                     KVYLNPQEIYKKMADANPALALLRKNLDMELD"
     gene            complement(10474..11523)
                     /gene="queA"
                     /locus_tag="PKH68_00340"
     CDS             complement(10474..11523)
                     /gene="queA"
                     /locus_tag="PKH68_00340"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNX11413.1"
                     /translation="MKLSQFKFKLPDELIAQYPAVHRDESRLMVLHRKTGEIEHKIFK
                     DVLNYFDENDLFVFNDTKVFPARLFGNKEKTGAQIEVFLLRELNREGRLWDVLVEPAR
                     KIRIGNKLYFGDDDSVVAEVIDNTTSRGRTLRFLFDGTHEEFKKELHKLGETPLPHNI
                     TRPVEPDDEERFQTIFARNEGAVSAPGAGLHFSRELMKRMEIKGINSAFVTSHMSLGN
                     FRDIDVEDLTKHKMDSEQMQITPETADIVNAAHDAGENICAVGVTVMRALETVVGTEG
                     RIKPLDGWTNKFIFPPYDFTVANSLISNFHLPYSTLLMLEAAFGGYEQVMEAYEVAIK
                     EKYHFGVYGDAMLII"
     gene            complement(11724..12416)
                     /gene="truB"
                     /locus_tag="PKH68_00345"
     CDS             complement(11724..12416)
                     /gene="truB"
                     /locus_tag="PKH68_00345"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNX11414.1"
                     /translation="MDFIEGEILYVKKPLSWTSFDVVNKVRWKISQKLNKKKIKVGHA
                     GTLDPLATGVMILCTGKATRRIEEFQYQTKEYVATLELGATTPSFDLEHEIDTRYPTA
                     HITPEMIDEVIHRFKGEIWQVPPVYSAVKIDGKRAYNYARDGQEVELKAKLLVIDEIE
                     VMSYDFPVLKIRVVCSKGTYIRALARDIGIALQSGAHLIALERTRIGDKTLEDCLDID
                     ELLKQIEEQDCY"
     gene            complement(12465..13349)
                     /locus_tag="PKH68_00350"
     CDS             complement(12465..13349)
                     /locus_tag="PKH68_00350"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNX11415.1"
                     /translation="MSLFETIIIAIVEGLTEYLPVSSTGHMIIAQKLLNVESTEFVKA
                     FTIIIQFGAILSVIVLYWKRFFKLNEIRIFDKNSVGGLTKCARSKVYFQRFLYKYDFY
                     WKLVIAFIPAMILGLLFNDLIDKMLESVMVVAVMLVLGGILMLFVDKWFNHPSENQTM
                     DWKRALKIGFFQCISMIPGVSRSMATIVGGMTTKLTRKNAAEFSFFLAVPTMAGASGL
                     KMVKMLTEPGGMDMLRENAITLIIGCVVAFIVAMAAIRFFIDFLTKYGFKAFGYYRII
                     VGTVIIGLLLAGLNLSMV"
     gene            complement(13352..13579)
                     /locus_tag="PKH68_00355"
     CDS             complement(13352..13579)
                     /locus_tag="PKH68_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNX11416.1"
                     /translation="MENKKFGLSKMNFILMAVAMLLIIIGFLLMTGKPSGTEFNPDIF
                     GTRRIIVGPMTSFIGFLSMIGAILYTPKNKE"
     gene            complement(13689..14579)
                     /locus_tag="PKH68_00360"
     CDS             complement(13689..14579)
                     /locus_tag="PKH68_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNX11417.1"
                     /translation="MSTKKHKKYKKSKLRFRNANLTATFSVSLVLFMIGLLTLTLFLA
                     RDMTRYVKENLNLSIVLSDEITPKQTERIKKFLEKSDYAKSVDLISKEQALQEHIQNL
                     GDNPEEFLGFNPLLASLEVKLNEKYANADSVSVLEKKLSKYKEIQKVSYQKDMITMVN
                     ENIKKISIILLGIAAILMLISFALINNTVRLSIYSNRFLINTMKLVGATHWFIRKPYI
                     KRSVLNGVIAAVIALAFLTGLVFYFQSEFGLQAQMITRNTQLIVAGVVVVLGILLTAV
                     SSYFAVGRYLRMRTNDMYYV"
     gene            complement(14634..17150)
                     /locus_tag="PKH68_00365"
     CDS             complement(14634..17150)
                     /locus_tag="PKH68_00365"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNX11418.1"
                     /translation="MNKTILNSLTPHIVAILLFIVLSFSYFTSVLEGKKLIAHDTQSW
                     VCDAKETLDYNETHDDVTLWTNALFGGMPNYQISMSQPNNVLQYVEKLVMEFPRPVSN
                     TILYLICFYILLLAFGLKPWMAVVGSIGFTFASYNFIIIAAGHNSKAITIAYMAPLIG
                     AVFLAFRRKRLLGGFLTAFFLSLAIRANHIQILYYTLIVLMIFAIVELVYSIREKQIK
                     EFLKTAVVMIAAAIVAVGMNATSLLTTYEYSQYTMRGKSNGLTAAATNSPSAVNTGHG
                     LDKDYITQWSYGVDETMTLLIPNFKGGASGGKLNMNSETAKYLKNLNVPDSQTEQIIS
                     QMPLYWGTQPGTSGPVYIGAIILFLFVLSLFVVDKRTLWWLVPVILLTLMLSWGRNFM
                     ALTNLFIDYFPMYNKFRTVSMTLVATGFGITLLAFLAMKEIFEGNIAREKLTKAVFIS
                     MGITAGISLIFAVFPSLAGSFVSPQDAQFTGDYSFLKQTLPLDRKAMLSADAWRSLAF
                     VLLGGIIILAYIKGYLKKNVALVLFGFFILLDLFPVAKRYLNDGNFENKRPTQLIQAT
                     PADEIVLQDKSQYRVMDATVDIFSDATPSYFHKNIGGYHAAKLRRYQELIEMQLQHEI
                     GQLFGAFGKATTPQSILPTLDSLGVLNMLNTKYIIYNKEAAPLVNPSNNGNAWFVQNI
                     RIAANADEEMKLVGEIDTKHELVIDKSLATMLPATITPDTTAKIELTSYAPNHLVYKF
                     NSTTDQVAVFSEIYYDKGWKATINGKEAPYTRVNYLLRAMPLKAGNYEIEFRFDPQSY
                     KTGNLVALLSSIILLAAIALLLFYYWRKKKSINNNTPTFK"
     gene            17422..18579
                     /locus_tag="PKH68_00370"
     CDS             17422..18579
                     /locus_tag="PKH68_00370"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX11419.1"
                     /translation="MTNYKNISLFEGLNSLRFFAAFLVVMHHSETIRHKNGLPNLNGL
                     GLFKDGGLAVTFFFVLSGFLITYLLLKENRQTGTISIKRFYLKRVLRIWPLYFLLVVL
                     GTLLLPKAIDLFGLNYVMPYTFGQSWYYFVFFLPGLVTYYFGHHLLEPLWSIGIEEVF
                     YMIWAPLFKFAKKHILPILIAIIVLKALLILISSYWIDNKLFAYLVSIFQFEAMAVGG
                     LGAYFVFNRNTPLSTLKIYKTPIQILLYSLLVVYLIFNINIDNAAWNIFFKTPVFSQL
                     MICFLFLYLIIGVSLIDHSIIRLRNRFLSYLGEISYGIYMYHMPLIFGLMMFLQKYLS
                     KMDLLTGSLIYYPVVFVVVVGVSALSKRYFEDYFLSLKNKISIPKAVTAKK"
     gene            complement(18619..20520)
                     /locus_tag="PKH68_00375"
     CDS             complement(18619..20520)
                     /locus_tag="PKH68_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNX11420.1"
                     /translation="MKRSLLTLSLALFGIILYSQSSPAKFNIIPEPVTTVEKSGNFTL
                     PKSVNILRPAGSENDITVNFLKEKLSKATGSRVTVKSSGKSDIQLVLNAIADPMIGNE
                     GYILSVRPKKITIRANKPAGLFYGAQTLIQLFPPQIESPTKVINVAWKIPAVEIGDYP
                     RVGWRGLMLDVARHFFTVDEVKQYIDAMVRYKYNMLHWHLTDDEGWRIEIKSLPKLTE
                     TGAWRVEKVGNFGEFSPALPDEPKTYGGFYTQDQIRDIVNYAAERFVQVIPEIDVPGH
                     SLAAVVAYPELSCTEGADKYTLRAGEPFMDWSQGAPPIALIDNTLCPANERVYEFMDK
                     VMTEVAQLFPYPYIHVGGDEAPHNFWQKNPQVQELMKREGLTTIPQVQSYFEKRLEKI
                     IQSRGKKMMGWDEILEGGITPTTALMSWRGTKYGIEASRSGHYVVMSPTTYAYIDYMQ
                     GDISTEPPVYSSLRLNKAYQFDPVPPEADARYILGGQANLWTEQVYNIRQVEYMTWPR
                     GFAIAESVWSPKEKKNWENFVNKTENHFQRLDYAETKYSPAIYDPIVSVTKKEDKYFV
                     ELTTEISGLDIYTSFDNSTPDRFYPRYKAPLLVPTDARLMRIITYRGKVPAGRLMTIR
                     VEDLKKRAK"
     gene            20752..21684
                     /locus_tag="PKH68_00380"
     CDS             20752..21684
                     /locus_tag="PKH68_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX11421.1"
                     /translation="MKQLHFSNGDRFPLMGLGTWLSKPSEVYNAVIEAIRAGYRHIDC
                     AYIYKNEKEIGDALKFAFSTGLVTREEMFITSKLWNNFHAPQDVEPAIRKSLSDLQLD
                     YLDLYLIHWPLAFRQEQAANAGGLISLAELPLTTTWKAMEDVKKSGLTRHIGVSNFNI
                     PKLKNLLENAEIKPEVNQVELHPYLQQRELVRFCQDNSILVTAYSPLGSHHLIDNENS
                     ILQNPTVMDIARKHYCSAAQVILAWGMHRSTAVIPKSVNPQRIQDNMSAEIIQLDDKD
                     MESIMSLDCNHRNAKALFAVFPDGPYTLKSIWDE"
     gene            21924..23147
                     /locus_tag="PKH68_00385"
     CDS             21924..23147
                     /locus_tag="PKH68_00385"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNX11422.1"
                     /translation="MTEQEIQHIRADFPILEEKIYGKQLIYFDNAATTQKPQAVLDRI
                     NFGYKHLNANIHRGVHYLSQKATEAHEEARRYVARYINSASSDEVIFTRGTTEAINLV
                     AFSFGEYCCQAGDEIIISVMEHHSNIVPWQMLCERKGMKLRIMPMNDKGELKLDEYER
                     LINEHTRLVAVTQVSNVLGTVNPVKEMISIAHQKNIPVLIDGAQAVPHIRVDVQELDA
                     DFYVFSGHKIYAPTGIGVLYGKRKWLERIPPYQGGGEMIQTVRFEKTTYNELPFKFEA
                     GTPDYIGSTALAEALKYVETIGIDKISAYENELTTYATAKLMKIDGMRIFGTSEHKSA
                     VISFLVGNIHPYDIGMLLDKLGIAVRTGHHCAQPLIEQLGIPGTVRASFSFYNTKEEV
                     DTFIAALKRVVGMLG"
     gene            23312..23734
                     /locus_tag="PKH68_00390"
     CDS             23312..23734
                     /locus_tag="PKH68_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNX11423.1"
                     /translation="MTINEIQDQIIEEFSQFDDWMDKYALLIDLGNTLQPLDDKYKIP
                     QNIIAGCQSRVWLNANLVEGKVIYEGESDAVLVKGIVSMLIQVLSGHTPEEILDTDLY
                     FIEKIGLREHLSPTRSNGLLAMVKQMKLYALAFKSSKT"
     gene            23962..24393
                     /gene="mce"
                     /locus_tag="PKH68_00395"
     CDS             23962..24393
                     /gene="mce"
                     /locus_tag="PKH68_00395"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNX11424.1"
                     /translation="MKVTHIEHLGIAVNSIEEALPFYENILGLKCYAVEEVADQKVKT
                     AFLKVGQTKIELLESTDPEGAVGKFIEKRGEGIHHLAFAVEGLADALKEAEEKGIRLI
                     DANPRKGAEGLNIAFLHPKSTIGVLTELCENPDKTPGSSCC"
     gene            24442..26001
                     /locus_tag="PKH68_00400"
     CDS             24442..26001
                     /locus_tag="PKH68_00400"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX11425.1"
                     /translation="MKNQEKVQDLIDKRVQAKLGGGEKRIDSQHKKGKLTARERIDML
                     LDDGSFEEFDMFVTHRSTNFGLDKEKYMGDGVVTGHGTIDGRIVFIFAQDFTVFGGSL
                     SETMAQKICKVMDMAMKMGAPVIGINDSGGARIQEGVTSLAGYAEIFQRNILASGVIP
                     QISAIYGPCAGGAVYSPALTDFILMTEDNSYMFVTGPKVVKSVTGEDITTEDLGGADV
                     HARKSGVTHFKLVDEKEGTMLIRKLISYMPQNNLEEPPLYPTTDPIDRLEDSLNSIIP
                     DNPNHPYDMKDIIHAIADDGEFLEVHRHYARNIIVGFARFNGASTGIVANQPNFLAGV
                     LDVDASRKAARFVRFCDAFNIPILTLVDVPGFLPGSGQEYAGIIIHGAKLLFAYGEAT
                     VPKVTVTIRKSYGGAHDVMSSKQLRGDFNYAWPTAEIAVMGAAGAIEVLEGRNIAAIT
                     DPEERANYVTQKELEYKEKFANPYEAAAYGYIDDIIEPRNTRFRVIRAFEALQTKKVI
                     NPPRKHSNMPL"
     gene            26038..26355
                     /locus_tag="PKH68_00405"
     CDS             26038..26355
                     /locus_tag="PKH68_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11426.1"
                     /translation="MLIAIIAVLAVALAVILYLFLQKNKAVKEIAAEKQSFKAQTAGE
                     NTVKSEVVPGNDEEVAAFLALHLYLTHNVHDVESNVITIERIQRRYSPWSSKIYSINN
                     FQR"
     gene            26393..26842
                     /locus_tag="PKH68_00410"
     CDS             26393..26842
                     /locus_tag="PKH68_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNX11427.1"
                     /translation="MRKYKFTINEKEYNVVVKGIDDEIAELEVNGNPYEVKIHQLEQK
                     VPKTPILVRKEVQNKPGENKVAEKLAPMPITAKPSSKTINAPLPGSVIKIVVEVGSVI
                     KEGDVLMVMESMKMENNILAEKSGTIVKVCVAPGASVMQDEALFEIQ"
     gene            27079..29547
                     /locus_tag="PKH68_00415"
     CDS             27079..29547
                     /locus_tag="PKH68_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX11428.1"
                     /translation="MENTTDFHDYARQFAELTNCNIFLTGKAGTGKTTFLHELRKNTG
                     KQIAVVAPTGVAAINAGGTTIHSFFQLPFTPFIPTAEGRKDLIGKLKMQSHRRKVIRE
                     LELLVIDEISMVRADLLDAVDTLLRHIRYRNNEPFGGVQMIFIGDMYQLSPVAQAEEW
                     RLLSPYYEGIYFFHSRVMQQKPPVYIEFDKIFRQKDMSFITLLSEIRNNNLSAAGLTM
                     LQSRYNPLFVPPKDDTYIILTTHNYKADRINAEELAALKVRSRYFNAEVKGDFPEKLF
                     PIEEKLELKTGAKVMFIKNDTETPRRFFNGKIGVITALGDETITVKCPGDDREIEVSP
                     MTWHNVRYKANETTLQIDEEVLGTFTQFPLRLAWAITIHKSQGLTFEKAVIDAGAAFA
                     PGQVYVALSRCRSLEGMVLLSPINQYSLQNDEQIVRFSDQTNTIDQLTTQLEVSKRNY
                     QETLLLGIFDFKPVVATANRWLNRAKDVASSFNKDTLPFIQNIIKQTEALQDVASRFQ
                     SQLIQINGTTPFNQELLTSRLQAANGYFSEHLSNLSETLKQTTATTDNRQNANEYDDF
                     LEDIYTYAEQKKHLIQGIQNGFSPEKYFDLKRSFELPKFSHSSYSKTNTNQRISSRHP
                     QLLNYLFQARNEISDNSGLPIYLIATVKSLTEMADYMPLNEKDLLKISGFGKPKVDKY
                     GEQFLNIIRRYCDDNNLSTEMSLFEEKAKKSSAKKEKKPKTDTVRVTLQFYLDGMSIA
                     QIAKERSLTEGTISKHLADLVEKGVLKVERFIGHNKLQQAVGLLEKGNLSGSVYELLS
                     NIMSQQEIIIMLGWIRGGKKEGKL"
     gene            complement(29584..30144)
                     /gene="ruvC"
                     /locus_tag="PKH68_00420"
     CDS             complement(29584..30144)
                     /gene="ruvC"
                     /locus_tag="PKH68_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNX11429.1"
                     /translation="MAKDQIILGIDPGTTVMGYGIIRVKDNKAEMLAMGVLELKKYAD
                     HYQKLQRIFARTLSLVDEFHPDCLAIEAPFFGKNVQSMLKLGRAQGAAIAAALYRDLP
                     VSEYAPLKIKMAITGNGRAAKEQVADMLRRYLRISQEDMLPQLDATDGLAAAYCHFLQ
                     SGKPSIDSEYKSWKDYINQNGTKVRK"
     gene            complement(30150..30461)
                     /locus_tag="PKH68_00425"
     CDS             complement(30150..30461)
                     /locus_tag="PKH68_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNX11430.1"
                     /translation="MAKFIYNTTYLVADRQFNPWFKWLTEVHIPNMLGTGSFTSPQIA
                     KVLTEDKEQEGVSVSVQFLVNDLITLQLWEENDAELVQNDLASRFGTEVLSFSTVLEL
                     F"
     gene            30533..30606
                     /locus_tag="PKH68_00430"
     tRNA            30533..30606
                     /locus_tag="PKH68_00430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30567..30569,aa:Ala,seq:ggc)
     gene            complement(30820..31716)
                     /locus_tag="PKH68_00435"
     CDS             complement(30820..31716)
                     /locus_tag="PKH68_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX11431.1"
                     /translation="MKTQILILSALILTASITVNAQSQAVKDTTLIFNQKQIHVTDSV
                     DQVKVQVYKTDTAEYKKIYEGIFTDENSFEKYSVETQFGFEFPFDKRKRSSKFSGHFI
                     GIGFGALNTTENFSNFNDAGGMKVVFSNEFTFNPIGYSLPVIKEYVGITTGIGMIWRN
                     LHLGNNSHLVNNNGITIPQAAPEGISYYSSRLRIFELNMPLYLEIHPVRKSEFYIMGG
                     ALFGINTFSSYKVKYKTDDNTKVKKVEGNDYNVNPFSLSYLVEMGWDDLGVFAKYTPT
                     TLFKKDKGPEVQTFTVGLSLNF"
     gene            complement(31725..32183)
                     /locus_tag="PKH68_00440"
     CDS             complement(31725..32183)
                     /locus_tag="PKH68_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11432.1"
                     /translation="MKMNSKCKYLILVVMFIAGCGLEIKAQSDLDINRIFDKYGKQKG
                     SVMVEMSKEMLEGYDFTFYKSIVISNNKEAGDFTRGCLQTDQNGAKKVKQVVTNGKIS
                     SVYLQLPKKGKYNRLILFNEKSVPELKMTLIYIESEKDSEEIMKLILKKK"
     gene            complement(32200..32667)
                     /locus_tag="PKH68_00445"
     CDS             complement(32200..32667)
                     /locus_tag="PKH68_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11433.1"
                     /translation="MKLNRRNIITVDEAEKLIEKYYEGETTVAEENQLRTFLAQSNVP
                     PQFEAEKAIFGYYESKKARKHFRLQPYLRWAAAAVIAAGVIIPVSIFQASGKTSYAYI
                     NGVRITNTQEIISLAQTTINYVSDNNEVEDGLDNLKNDETIKTQLDAFSEIEL"
     gene            complement(32664..33161)
                     /locus_tag="PKH68_00450"
     CDS             complement(32664..33161)
                     /locus_tag="PKH68_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX11434.1"
                     /translation="MSSELFKKEILPLRHKLFRQSLKMLDDSEDAEDAVQEVLLKLWS
                     KRDTLITCDNPAAFATTVTKNHCLDKLKLKKETETIDNLYAVSARDNPHLQLERKNTE
                     QTLKHIIGTLPQLQQQIITMKDVEEYEIDEIAEITGTSYEAVRMNLSRARKKVREEYI
                     KLTQR"
BASE COUNT         9691 a         7256 c         6880 g         9359 t
ORIGIN      
        1 tcttgtttag cagcttgaaa gcactcatac tgtagtaagt tacagtttcc ttttatatcg
       61 aactcatttt aaaaacagta atccccttta cgttcatgtc gtaaagggga ttgctttgtt
      121 ttatagtacg ttgaatttat cttcctgatt gcacgatgaa atttctcagt tcattattaa
      181 attcatcttc ctgcaagagg tcttccatat tcagatgcaa gcggtagcgc cagctgctca
      241 tggagttgga aggaatgttg atgcgttctt cgtgcggatt ttcacgtctc agtttgggag
      301 aaatacccaa caaatcctgc atttggaaga ttgcccacat agccggtgaa tacagatgct
      361 gcaatattat atcacgactt atccaccatt cacagaagta tggtgcttca ccccagtggc
      421 cgagttgggt gttgaagaat ttttgtgtta ccgagcgatc ttcttcccac cagccgcgta
      481 cggtactcat atcgtgggtg gatggtgtaa ctacagacag gtacggagca tcattgggat
      541 gaaaaaactc tattttattg cttttcggtg cacgctgcac ttccagacta agtatgccca
      601 attctttcat cacagccgtt acgcacggtg tcatcatacc cagatcctct ccacagataa
      661 gcatattggt ggagcgtttg agtgccggga gtttttccat gcctgagtga taccagtttt
      721 catcctgacg atggtaaaaa taatgaatat aaagttcatc cagccctttt ttggtatgat
      781 cgtcgagttc aatgtaagaa cgcaggctgt gcattccgta ccgcggataa aactgctgcc
      841 cgttagaacc tttcacttcg aataacaaca cattggatat cagatcaaac agtccccatt
      901 taacccgttc ggagattttt ccttcattga agagttcgtc cacataggtc tggctctgaa
      961 actcaggttt taaccgtagt acccagccgt cttctatctg taaacagttg gctttcaccc
     1021 aggcagattc ctcgccaaag aaatcccata gtatctggtc ggtgatgtaa ggtttgcaga
     1081 aacggttata atcaaaccag ataccttttt caccaaattc attgatataa ataggtgtag
     1141 aaggattgag atgtcccata ataccttcaa gctggttggt gggtatttcc cagatgcgga
     1201 aaaatcccag aatatgatcg atgcggaacg tatcaaagta gtttgacatt tgctcaaagc
     1261 gttttttcca ccaaccaaat ccggttcttt tcatcgcctc ccaatcgtag gttggcagtt
     1321 cccagttctg ccccttgatg gcaaacatat cgggcggagc accggcattc atgtccatgt
     1381 gaaataactc aggcgatacc caggtatcca cgctgttttt atttatacca atggggatgt
     1441 cccctttcag caccacacca ttttcgtgtg catagcgtgc agcctccgaa agctgtttgt
     1501 gcagatgata ctggataaaa taattcacac ctacttcgtc gaaatgaggt tgtgtttttg
     1561 cagtaaatgc agcaatgagt tccgcattat agacagcata ttcgttccat tggcggtagt
     1621 cattggttcc aaatttatca cgcattacac agtaagcggc atatggtacc agccaatggg
     1681 catttttatc aaagaatgca agaaaatcag catctttgag gaattcttcc ttttgataca
     1741 gatacgcctc tcgggcatat tgaagtttga aatttatcac ttcgagaaaa gaaaccaacg
     1801 gaagggcatt gagttcggcc tgtttgggta tgtattgctg cgccaggctg ttagtttcgg
     1861 gtaatttgcc taacgccggc agatggagaa acagcggatt aagtgcaaaa gccgaaatgg
     1921 ctgcataggg taatacatcg gcatcggtat gggtgccatt cgaatcattc agcggtaaaa
     1981 cctgaataag tttcatattc acctttaccg cccaatcgat aaacagtttc atatcttcaa
     2041 aatcgcccac cccaaagctg cgacgggtac ggatactgaa tacaggaaga cttacaccag
     2101 ttccacgcca cgaattgttg ttcatacgca caaaaccgtc gtgaacctgc accaacgtat
     2161 tggcagagcc tattacagcc gctatccggt cgggtccctg ctcaaagaac ataaaatgct
     2221 gttcatcaaa atcataaatt ccatatttat aatgaacttc ggatttaacc ttactcaggt
     2281 caacttttac aatccagttc tcgccctcct tatacatcat caatggctcg gtcactgacc
     2341 aactgcccaa ttccttgcag ttgccaatga tgcacaatgt ttgccgggca tgcagcaatg
     2401 gagctttaac cttaaaaata tgggtgaatt ttttatttct ttttggtggt gcagtggcgg
     2461 tattaacttt cagcagcact tcctgaaaag gacttgtcat gaatacattg tcgttgctgc
     2521 tcaccgaatt ccaggtatcg aagcagaaat aatgttcggg attattggag taaacagcta
     2581 catttcggtc atcgccccac tcttcggtgt aactgctgtt ggtttcgtcg tacagtaaat
     2641 acttataact gatcacctga ttttcggtga cggggcaatc cagttcggca acccaatctt
     2701 catgtccttg aaaccggagg ggtaatgccc gggaaaaatc gtcgtttccc agttcgggaa
     2761 tatttccgct aaccagaatc cgttgtcccc attttgtaaa ataggataat ttaaaatgta
     2821 ttttcatggg tataaatgtg tttttgacac gttaggtttc gatttgtgat aatcaaaagg
     2881 gtaaaactct gctcctatct gattattaca atgcttactg aaacattttc tcaaaataat
     2941 attcaaaaat agtggataaa ataaattcgt ttactcattt tgagataaaa caagattaaa
     3001 ttgatttcaa acgattgcga gaactaaaat tacgatcgtt actaaaattc ggggttagta
     3061 tattcttatt attcagagac ataaaacctg ataaaaaaaa atagggaatt aatagggaaa
     3121 ataatttccg gtttaatcca aaataagtac ttttgtgaaa aatatcatct aacctctaat
     3181 catctaattt atgtcagaag attccaccct tttcaaagta ttttcgggaa caaagagccg
     3241 ctatttagcc gaaaaaatct gtaaaagttt aaactggcct cttggaaaga tgaatatcga
     3301 acacttttcg gacggggaat tttccgtgtc gtacgaagaa tctatccgcg gacaatacat
     3361 ttacctggtt caatccactt tccccaattc ggataacctg atggagcttt tgctcatgat
     3421 tgatgctgcc aaacgggcat cggcttacaa ggtcattgcg gtaatacctt attttgggtg
     3481 ggcacggcag gaccggaaag acaagccgcg tgtatccatc ggagccaagt tgatagctga
     3541 catgctcact gtagcgggag ttgaccgcgt catcaccatg gatttgcatg ccgatcagat
     3601 acagggtttc ttcaatgtac ccgtcgatca cctgtatggt tccaccattt ttatccccta
     3661 tatcaaatct ttaaatcttg aaaatctggt aatcgcttcg cccgatgttg gtggctccaa
     3721 gcgtgccggt tcctactcca aacacctgga agtgcctatg gtactctgcc ataaaacacg
     3781 tgagaaagcc aatgtggtag gtgagatgag aataatcggt gatgttgttg gtaaaaatgt
     3841 agtcattgtg gacgatatgg ttgacaccgc cggcacactc tgcaaagcag ccaacctgat
     3901 gattgaaaac ggagctgtta gcgttcgggc cattgtttca cacggggtca tgtcgggatc
     3961 tgccatcaaa aatataacca attctgccct gaccgaaatt gcatttacag attcgatacc
     4021 gtttgattgc aataaatgtg agaaagcaaa aattctaacc atttcagata tgtttgccga
     4081 taccattcgc agggtacagg aacacaaatc catcagcgaa caatacctgt tgtgatctga
     4141 agtcagaaat tagaagtcag aaattagaaa ttagaaataa aaaactgccc tttgtcaata
     4201 tttttgacaa agggcgtttt tttggcacga agtttggtca acaattaaat acgacagaat
     4261 ttccttttac cccaacataa actaagattg atatgaaaaa tctggcagct ttattcgttg
     4321 tcctgctttc cgtgatgttc actttaccgc agcagttaca ggccacacca cccgactggg
     4381 cacctgccaa cggataccgt gatcaaacaa ggcacatcta ttttccacag caaaacattt
     4441 attacgacct acagaaagaa gtgtacatgt acgacgacgg tggtctgtgg tttgttatta
     4501 aatccgttcc ggaaaaatac aaaaacatta acctgcggaa atcgcctcag gtacagttgt
     4561 tcctgaatac cgacgctcca tataccgaga atgagactca caggacaaaa tacaaatacc
     4621 tggtacacga gagcgaaaag acaataagag aaactgaaaa attgataaga aaagagcaga
     4681 aagcgcaaga aaaagcggag agaaaatcag aaaaaaaata gctcagatac caactcagaa
     4741 aaaataacga aagaaagaaa aataaaattc tccggtcgtc aatcagcggt cggtcaatat
     4801 actaaacctt tttttaattg gatactccgg catattaacc tgccggagtt ttttttcttc
     4861 tactgaaaca aatcctatgt agctttacat actaaaatac acgtaataat attggtcgtt
     4921 aactcaactt ctggtgacag aattttttcc tgtaaaattt cctttatctt tgtgacctaa
     4981 taaaaaagta gagtatgcat acccgaaaaa tatctgttct gttcctgatt tttatttccc
     5041 tgacattgtc tgcttatgct caacgctata caatcagtgg atacattacc gaccagtcga
     5101 acggtgagac gctgctgaat gcctcggttt ttgacgacgt ttcccgaaaa ggcgctgtga
     5161 gcaacaatta cggacattat tccattacac tgaatgccgg tgatgtaagt ctcaattaca
     5221 gttatgtggg atattctcca caattcagag agttcaaact gaccaaagac accgtcattc
     5281 acgtaaaact ggaggcaagc attaccctgc gggaagtaac ggtaactggc cgacgcagcg
     5341 aagtgggcgt acacagcaca cagatgagcg ccatcgaggt gcccattgag caaattaaaa
     5401 atattccctc tctgtttggc gaaaatgacc tgataaaagc cctgcagctg ctgccgggag
     5461 tgcagtcggg caccgaaggt tcggccggat tgtatgtgcg cggtggcgga cctgatcaaa
     5521 acctgatgct gctggatggt attccgattt acaacgtcaa ccatatgttc ggattctttt
     5581 cggctttcaa taccgatgcg gtaaaaaatg taactttgta caagggaagt tttccggcac
     5641 gatttggtgg aagactctcg tcggtagtgg atgtgcgtat gaaagacggc gacgataaaa
     5701 actaccacgg caatgtaagc gtgggactta tttcttccaa atttaacctc gaaggtccga
     5761 tttggaaaga aaaaaccacc ttcaacattt cggcacgacg cacctatatt gatgtcctga
     5821 gccagcccat catcaggtat tattcccgac aaaacatggg agaggatgcc gggagcacca
     5881 ccgccggtta ctatttctat gatctgaatg ccaaatttac ccataagttt tccgaaaaag
     5941 ataaactttt tctgagtgcc tacctgggcg acgatgccat ctataccaaa ttccgaactg
     6001 acaaaacttc gtatggcgaa agaagtgaaa acgactggat gaacctgaac tgggactggg
     6061 gaaatgccat tgctgcactg cgatggaatc acgtggttac caataagttg tttatgaaca
     6121 taacgggcgc ctacacccgc tatcgttcca acatgaatgt aggatacctt tcagaaagta
     6181 cggataaaac caccaatcca cccaagacac agaccgaaga gttcaaagtg ggctaccgct
     6241 caggtataga ggattattcg ctgaaagccg attttgatta ttcacccaat acatcacacg
     6301 atataaaatt cggcaccaac tatacgtacc acacattccg tcccgatgta tctacgttct
     6361 attttttcaa taataacaat agtcaaaatc agaaaattga caccgtaata ggcaaaggaa
     6421 atatctatgc acacgaatca atgacttacg tggaagataa tatcacactc actgacaggt
     6481 tcaaaattaa tgcaggcatc catttctcaa ctttcaatgt acggcagaaa aattattatt
     6541 cactcgaacc acgtttggga tttcgtgctt tgcttaccga ttatctctcg ctcaaagccg
     6601 gctatgcaca catgaatcag tacattcacc tgctttcgaa caacagcatc agcatgccta
     6661 ccgacctgtg ggtgcctgcc acgcagctca tcgacccgat gaagtcggac cagtatgcat
     6721 tcggcatatt ctacaacctg aaaaatctgt ttgatctttc cctcgaaggg tactacaagt
     6781 ccatggataa cctgatagaa tacaaggatg gtgcatcgtt tatgggtgaa tcatcgggtt
     6841 gggaagaaaa agtggtgatg gggcgcggat ggtcctatgg cgtggaattt ctggcacagc
     6901 ggtcgtttgg gaacactacc gggtggcttg gttatacctg gtcgaaaacc gaacgactct
     6961 tcgaccgccc cggacaggag cttaactata gtcgaatttt tcccgctaaa tacgaccggc
     7021 gtcacgattt aagtctgacg gtaatgcaca aattctccga aaaaattgac ctttcgggca
     7081 actgggtatt tgctacggga aatacagcca cactcgggct gcacaccaac gaaggaccgg
     7141 tgataccaaa cgtaaacgag gcttacaact accctgcccc tcagacttac atcagttcgc
     7201 gcaacaacta ccgctacaac aattaccacc ggatggacct gggtgtgaat tttcataaaa
     7261 agaaaaaaca cggcatccgc acctggaata taagcatata taatgtttat aatcagttca
     7321 atccatttat ggtttatcag tcgtcggagg gtagttacga cgcaagaacg ggtcgatacg
     7381 agagcaaaaa cgttttgaaa caggtaaccc tgttccctat cattccatct gttagttaca
     7441 gttataaatt ttaagaaaat gaaaacgaaa catattttag ctgttttgat tattggccta
     7501 ctcacctgcg gatgcgacaa aacaattgaa ttcaacggga aaatcaccag cccaatgctg
     7561 gttgtgagca gctttgtcac acccgactct gttatcaaag ccgaagtgac caaatcacgc
     7621 ttctttttgg atgagggtta tgtgtttgaa agggtaacga atgccgattt taatttgtat
     7681 gtaaatggca tactgaaaga aaaaatggtt cacacgggat acggaattta tctgtcgggt
     7741 tacagacctc atccgggtga ggaaatcaaa atagaagttg ctgctcaggg gtttaccaca
     7801 atatcaggca cttcagtaat acccgcacag actcctattc tgaaagtaga cacttcttct
     7861 gtcattgaaa aagaatatta cattggcagt aaaggcggat atggaggaat gaattcacaa
     7921 gatacactct cggaactcac cctgaaaaaa atcagttttc gggtaaagtt caaagatgat
     7981 ggtgataaaa gaaactatta ccgcctggtg gtgagaaagc ggatctatta cgaagattct
     8041 gctttggatg tgtatctggg taattttgag gacattgttt ttggaaatca gcaaaccaat
     8101 gacataggaa gcctttttga gttttcgaga aacaactaca actacgatac ttttgctgat
     8161 gatttattaa atggaaaaga gtacgagttg aaattttaca acataataac tgttgatcag
     8221 aaagactttt caggctacga gtcccaattt cgcaaagaac tgcgaagcac ctacttcatt
     8281 tatttacagc aaatttcacc ggattattat ctgtatgccc ggtcatcaag cgctgccgca
     8341 aatttaagcg gaaatccttt tacagaaccg gttcaaattc attcgaatat caaaaatggt
     8401 atcggtgtgc tcggcagcta tacatcatcg gcagcagcgg tggtggatgt ggtgaactga
     8461 cgttttcggg gttgcatgcc gactgcgctg caagtattta agcagaaaaa aaactgattg
     8521 cccgtttaca attcccgaca gtaaagtcgc tatttgattt gtgctgaaaa aattgtacat
     8581 ttgtatttcg ttttttaatt tcaaaaaaag tatcttttaa tggaatcatt ccttgtttca
     8641 gcccgaaaat atcgccctgc cacctttcga tcagtagtgg gtcaggaatc gcttaccact
     8701 accctgcgca atgccattaa aagcaataaa ctggcgcacg cttatctttt ctgcggtccg
     8761 cgtggtgtgg gaaaaaccac ctgtgcacgt atttttgcca aaacaatcaa ctgcgaacat
     8821 ctcaccgaag attacgaagc ctgcaaccag tgcgaatcgt gcaaaacttt caacgagcag
     8881 cgctcctaca atattcacga gctggatgct gcatccaaca acggcgtgga cgatatccgc
     8941 agccttaccg atcaggtgcg cgtaccaccg caggtaggac gatacagcgt ttatatcatc
     9001 gatgaggttc acatgctgtc gcagggagct ttcaatgcct ttctaaaaac tctggaagaa
     9061 ccgcctgctc atgccatatt cattctggca accactgaaa agcataaaat cataccaacc
     9121 atactttcgc gctgtcagat atatgatttc aaccgcattt ccatcaccga cacggtcaac
     9181 cacctgaaat atgttgccgg tgaggagcat gtgacgattg aagaaagtgc tttgaatgtt
     9241 atagctcaga aaagtgatgg cggaatgcgc gatgcactct ctatattcga ccaactggta
     9301 agtttcagtg gcaataacat cacctatcag gatgtgattg ataacctgaa tgtattggat
     9361 tatgaatatt attttcgcct ggtgaatgca tttctgcatg gtaatgtggc cgaatcgctg
     9421 ttgctattca acgaagtact caataaagga tttgaagcac aacactttat taccggactg
     9481 agttcacatc tccgggatgt gctggtgagc aaggatgcca ccaccttaca gttgctcgaa
     9541 gtgggtgccg aaatcgctga acgttaccgt cagcaggctt cggcatgtcc gacggaattc
     9601 atattcgatg cattgaaaat tagtaacgat tgcgatctga attaccggat cagtaaaaac
     9661 aaacggctac tggtagaact ggctctaatt cgtatcagtc aactcacgac tgataaaaaa
     9721 aaagtactga ccgctgagaa agcaccgcta aaaccactgg aagcagctga aacagttact
     9781 accaaacaac ccgggcaagt aggttcacaa accgcatcag cagccactcc tccgcagcaa
     9841 atccaaccac cggctgagtc tgcaccggcc gaaataacgc caacagccgc cacaagcgcc
     9901 aatccggtag aagtgacagt agctcccaga aaaccggctg aggtatcggt aggaaaaata
     9961 aatcgtcccc caagtatctc catatcatct atcaaaaccg aacctaccgg aatcagcaaa
    10021 ccggatattg tctccgacac cgagggagtt tttgccactg ataaaccatt tacagcagca
    10081 gaacttactg atgcctggaa attatttgcc ggtcggtttg aaaaagatca ggttaccagg
    10141 ggatttctgc ttagccatat tccgaccata gggatggata ataacattaa cttatcgctg
    10201 gataataaat ttcaggaaaa agaaatcagt cagatgcatg cgttggaatt tttgaggaaa
    10261 acactacaca acaattccct gaattttcag attgatatta ttcccgatga ggaacaaaag
    10321 gtctacctga atccacagga aatttataaa aaaatggcag atgcaaatcc ggctttagcg
    10381 ttgcttcgca aaaacctgga tatggagctt gactaaacaa aaaaatccgc tgaaattatt
    10441 caacggattt ttttgtttga agttaatggt ttatcaaata ataagcatag cgtcgccgta
    10501 cactccaaaa tgatattttt ctttaatggc aacttcatac gcctccatca cctgttcgta
    10561 gcctccaaat gcagcttcca gcataagcaa tgtggagtaa ggcagatgga aattggatat
    10621 cagcgaattg gcaacagtaa aatcataagg aggaaaaata aatttattcg tccatccgtc
    10681 caggggttta atacgacctt cggtacccac taccgtttcc agtgcccgca tcacagtaac
    10741 gcctacagca cagatgtttt cacccgcatc gtgggcagcg ttcactatat cggcggtttc
    10801 aggggtgatt tgcatctgct ccgagtccat tttgtgcttg gtgagatctt ccacatcgat
    10861 gtccctgaaa ttacccaaac tcatgtgcga ggtaacaaat gcgctattta ttcccttgat
    10921 ttccattcgt ttcatcaact cgcggctaaa atgcaatccg gcaccgggcg ctgaaacagc
    10981 tccttcgttc cgtgcaaaaa tagtctggaa acgctcttcg tcatcgggtt caaccggacg
    11041 ggttatattg tggggaagcg gagtttctcc cagtttgtgc agttcctttt tgaactcctc
    11101 gtgtgtgccg tcaaataaga aacggagtgt gcgaccccgc gaagtggtat tgtcaatgac
    11161 ttcagccaca acagagtcat catcgccgaa gtaaagttta tttccaattc ttattttgcg
    11221 ggcaggttct accagtacat cccacagacg tccttcccgg ttaagctccc ggagcagaaa
    11281 aacttcaatc tgtgcacccg ttttttcctt gtttccaaac agacgggccg ggaaaacctt
    11341 ggtatcattg aaaacaaaca agtcattttc atcaaaataa ttaaggacat ccttgaaaat
    11401 tttatgttca atttcaccgg tttttcgatg caaaaccatg agacgtgatt cgtcccgatg
    11461 aacggccgga tattgagcaa tcaattcatc cggcaatttg aacttaaatt gagataattt
    11521 catattcttt aatttacttc gttttacaag ttttattgct tatatataga tgcactatct
    11581 ttttttttat aataaaaaaa acatacaatc aaattcactg ttttctcaaa tttctcagaa
    11641 aaacagattc acataaccat tacagatgga ttgataccag taactcaatc actaagtttt
    11701 tgtaccaatg acttaatatc caattagtaa caatcctgct cttcaatttg tttcaataat
    11761 tcgtcgatat cgagacaatc ttcgagagtt ttatcaccga tacgggttct ctccagtgct
    11821 ataagatgtg caccgctttg caatgcaatg ccgatatccc gggccaaagc gcggatatag
    11881 gtacccttgc tgcatactac ccgtatcttt aaaaccggaa aatcgtaact catcacttct
    11941 atttcatcta tcaccagcag ttttgctttc agttccactt cctggccatc acgggcatag
    12001 ttgtaagccc ttttaccatc aatttttacg gccgaataca ccggtggcac ctgccagatt
    12061 tcacctttga agcggtgtat cacctcatcg atcatttcgg gggtgatatg agcggtagga
    12121 taccgtgtgt ctatttcatg ctccaggtca aaggaaggcg ttgtagctcc caattccagg
    12181 gttgccacat attccttggt ctgatactgg aattcctcta tgcgtcgggt agctttgccg
    12241 gtgcatagta tcattactcc ggtggccaac gggtccagcg tgccggcatg tccgacctta
    12301 atcttttttt tattcagttt ttgtgaaatt ttccagcgta ctttgttcac cacatcgaac
    12361 gatgtccagc tcaggggttt tttcacatac aatatctcac cttcaataaa atccatcaac
    12421 ctgttgttac tttaatttta tttgataaaa actaccgaaa aagtctaaac catgctcaaa
    12481 ttcaaccctg ccagaagcaa gccgataatg acagttccca caattatccg gtaatagccg
    12541 aaagccttga aaccgtattt agtaaggaaa tcgatgaaaa accggatagc agccatcgct
    12601 acaataaatg caaccacaca gccaatgatc agtgtaatag cattttccct gagcatatcc
    12661 attccgccgg gttcggtgag cattttcacc atttttaatc ccgaagcacc tgccatggta
    12721 ggtactgcga gaaaaaacga gaattcagcg gcattttttc gggtcagttt ggtggtcata
    12781 ccgcccacaa tcgtagccat agagcgtgac acaccgggta tcatggaaat acactgaaaa
    12841 aaaccgattt tgagggcccg tttccagtcc atcgtttgat tttcggacgg atgattgaac
    12901 catttatcca caaaaagcat caatattccc ccgagtacca gcatcacagc caccaccatc
    12961 acactttcga gcattttgtc aatcaaatca ttgaacagca gtccgagaat catggctggt
    13021 ataaatgcaa tcaccagttt ccagtaaaaa tcatatttgt acagaaaacg ctgaaaatac
    13081 acttttgaac gagcgcactt ggtgagacct ccaacggaat ttttatcaaa tatccttatt
    13141 tcattgagct taaaaaaacg tttccagtac aatacgatga cagacagaat agccccaaac
    13201 tgaatgataa tggtgaatgc cttaacaaat tcggtactct ccacgttgag caatttctgg
    13261 gcaataatca tgtgtcccgt tgaggaaacc ggcaagtact cagtcagtcc ttcgacgatg
    13321 gctataataa ttgtttcgaa taaactcatt cttattcttt atttttcggg gtgtaaagaa
    13381 tggcgccaat catggataaa aatccgatga acgatgtcat gggacccaca attatgcggc
    13441 gggtaccgaa tatgtcggga ttgaattcag tgccggatgg ttttccggtc atgagtaaaa
    13501 acccaatgat aatcagcagc atagcaacag ccattaggat gaaattcatt ttggaaagtc
    13561 caaatttttt attttccatt tttattttga attaaaatag ttcgaaaagg ttagaaagtg
    13621 aattttgcat gcctgaataa aataaataca gcaaaattaa taaatatgaa cattaaacta
    13681 agtcgacact aaacgtaata catatcgttg gtgcgcatac ggaggtatct tcccactgca
    13741 aaataggacg aaacggcagt aagtagtatt cccagaacaa caaccacacc ggcaacaatc
    13801 aattgtgtat tgcgggttat catctgcgcc tgaagaccga attcactctg aaaataaaat
    13861 accaaaccgg tcaggaaagc aagtgcaatg actgcagcta tcacaccgtt gagtacactg
    13921 cgtttgatat acggtttgcg gataaaccag tgagtggcac ccaccagttt catggtattg
    13981 ataaggaaac ggtttgaata aatgctcaga cgcacggtat tgttaatcag tgcaaacgat
    14041 attaacatca gaatggccgc tattcccagg agtatgatgc ttattttttt tatattttcg
    14101 ttcaccatcg tgatcatatc tttctggtac gataccttct gaatttcctt gtatttcgac
    14161 agtttttttt cgagcaccga tacactgtcg gcattggcat atttttcatt gagtttcacc
    14221 tcgagtgatg ccaggagtgg attgaaacct aaaaattctt cgggattatc gcccaaattc
    14281 tgaatatgct cctgcagtgc ctgttctttt gatatcaggt ctaccgattt ggcataatcc
    14341 gatttttcca gaaatttttt aatccgttca gtttgttttg gtgtaatttc atcactcagt
    14401 acgatggaga gattgagatt ttctttcaca taccgggtca tgtcacgtgc cagaaagagg
    14461 gtaagggtga gtaaaccgat cataaacaga accagtgaca cactgaatgt ggctgtaaga
    14521 tttgcgttgc gaaaacgtaa tttggatttc ttgtacttct tgtgtttttt tgttgacata
    14581 atatcttttt attccaaaac tgtcggccac cggggcttcc aattggacat tgtttattta
    14641 aatgttggtg tattgttgtt tatcgatttc ttttttctcc aatagtagaa cagcagtaat
    14701 gcgatggctg ccagcaaaat gattgaagat agaagagcca ccagatttcc cgttttgtac
    14761 gattgaggat cgaagcggaa ttcaatttca taatttcctg ctttcaatgg catggcacgc
    14821 aacaaataat ttacacgggt gtaaggcgct tcttttccat tgatagtagc tttccagcct
    14881 ttatcataat atatttccga gaaaacggcc acctgatcag ttgttgagtt aaatttatag
    14941 accaaatggt ttggtgcata actggtgagt tcgattttag ccgttgtgtc gggcgtaatt
    15001 gttgccggaa gcatggttgc cagcgattta tcaatcacca gttcatgctt ggtgtcaatt
    15061 tctcccacca gtttcatttc ttcatcggca ttagctgcta tccgtatatt ttgcacaaac
    15121 caggcattgc cattgttcga aggattaacg agaggagctg cttctttatt gtaaataatg
    15181 tatttcgtat tcaacatatt gagcacaccg agcgagtcaa gcgttggaag aatggactga
    15241 ggagtggtag cttttccaaa agcaccgaat aattgaccaa tttcatgctg caactgcatt
    15301 tctatcagtt cctgatagcg ccggagcttg gcggcatgat aacctccgat gtttttatgg
    15361 aaatacgaag gtgtagcatc gctgaaaata tccactgtag catccattac gcggtattgg
    15421 gacttatcct gaagcacaat ttcatcagca ggtgtagctt gtatcaactg tgttggacgt
    15481 ttgttttcaa aattgccatc attcaaatac cgttttgcta ccgggaataa gtcgagcaaa
    15541 ataaagaagc cgaataaaac cagggcaaca ttttttttga ggtatccctt gatatatgca
    15601 agaataatga taccacccaa caatacaaac gcaagcgagc gccaggcatc ggcactgagc
    15661 atggcttttc tgtccagtgg cagtgtttgt ttcagaaagg agtaatcgcc cgtaaattgt
    15721 gcgtcctgcg gtgacacaaa acttccggcc agcgacggga acactgcaaa tatcagactg
    15781 ataccggccg taattcccat actgatgaag actgcttttg tgagtttttc ccgtgcaata
    15841 tttccttcaa aaatttcttt catcgccaga aatgccagca gtgtaattcc aaatccggta
    15901 gcgaccaggg tcatggaaac ggtgcggaac ttattgtaca tggggaaata atcgataaac
    15961 agattggtaa gcgccataaa gtttcgtccc cacgaaagca tgagtgtgag taaaatcacc
    16021 ggcacgagcc accacagcgt gcgtttatca accacaaata agctcagaac aaacaggaac
    16081 aggatgatgg ctccaatata aaccggaccc gatgtgcccg gctgggtacc ccagtagagc
    16141 ggcatctgac tgattatctg ttcggtctgg ctgtcgggta cattcaggtt tttcagatac
    16201 ttagccgtct cgctgttcat gttcagttta ccgccgcttg ctcctccttt gaagtttggg
    16261 atgagcaggg tcattgtttc atccacacca taactccatt gggtgatata gtctttgtcg
    16321 agcccgtgtc cggtatttac tgccgagggt gaatttgtag cagctgcagt cagtccgttg
    16381 gatttaccac gcatggtata ctgactgtat tcgtaggtgg taagcagtga ggtggcattc
    16441 atacctacgg ccacaatggc agctgcaatc ataacgactg ccgtttttaa gaattccttt
    16501 atttgttttt cgcgaatgct gtacaccagt tccactattg caaatatcat cagcacgatg
    16561 agcgtgtagt ataaaatctg aatatgattg gcccggatag ccagacttaa gaagaaagct
    16621 gtgagaaatc cacccaacag gcgtttccgg cgaaaggcga gaaatactgc accaatgagc
    16681 ggtgccatgt aggcaatggt gatggctttt gaattgtgac cagctgcaat gatgataaaa
    16741 ttgtaggatg caaatgtgaa accaatggac ccaaccacag ccatccaggg ctttagtccg
    16801 aaagccagca acaggatgta gaaacagata aggtaaagaa tggtatttga aaccggtcgt
    16861 ggaaattcca tcaccagttt ttccacatat tgcagcacgt tattgggctg cgacatggat
    16921 atctgataat tgggcatacc gccaaataac gcattggtcc aaagggttac atcatcgtgt
    16981 gtctcattgt aatcgagtgt ttctttggca tcacacaccc agctttgagt atcatgggca
    17041 atcagtttct tcccttccag taccgaggtg aaatagctga atgaaagtac gataaagagc
    17101 agtatagcaa caatatgagg tgtaagcgaa ttcaggatgg ttttattcat acttttacgg
    17161 ttctgtattg aaaaaacagt ttttggggaa aatcacacaa aagtactaca ttttttccgt
    17221 tttgctacct gtgacaacgg ttaatattaa ttaattgaac ataggaaaaa aaataataac
    17281 agcattacta aacacctcac aacttcagga gtaaccggcc tggaaagaga ataaatattt
    17341 gtaaataatc aaaatctgtc ggaatttaat tgccggaatt atctactttt gccgttcgaa
    17401 tgtgatgctc atccattatt tatgacgaac tataaaaata tcagtctttt tgaaggatta
    17461 aattctctgc gtttttttgc agcatttctg gtggtgatgc atcattcgga gaccatccgc
    17521 cataaaaacg ggctgcccaa cctcaatgga ttgggactgt tcaaggatgg aggtttggct
    17581 gtaactttct tttttgtact cagtggtttt ttgatcacct acctgcttct gaaggagaac
    17641 cgacaaaccg gaaccatcag catcaaacgc ttttacctga aaagagtact gcgaatatgg
    17701 ccactttact ttctgctggt tgttttaggt acgttgctgc tgcccaaagc cattgactta
    17761 tttgggttga actacgtgat gccttatact ttcggtcaga gctggtatta ttttgtgttt
    17821 tttctgccgg gtctggtgac ttactatttt ggacatcatc tgcttgaacc gctttggtcc
    17881 atcggtatcg aggaggtatt ctatatgata tgggcaccgc ttttcaaatt tgcaaaaaag
    17941 cacatactac caatattaat agctatcatc gttttaaaag ctttattaat cctgattagc
    18001 agttattgga ttgacaataa acttttcgca tatctggtga gtatctttca gtttgaagca
    18061 atggctgtag gaggattggg cgcctacttt gtattcaacc gcaacactcc actctctaca
    18121 cttaaaattt ataaaactcc cattcaaata ctgctttatt ccctgctcgt ggtatatctg
    18181 atattcaata tcaacattga caacgctgcc tggaatatct ttttcaaaac tccggtgttt
    18241 tcacaactga tgatttgctt tctgttttta tatcttatca ttggtgtctc gctcatcgac
    18301 cacagcatca tccggttgcg caatcgtttc ttgtcctatc tgggtgaaat ctcctacggc
    18361 atttatatgt atcacatgcc actgatattt ggtctgatga tgtttttgca aaaatacctg
    18421 tcgaaaatgg acctgctgac cggttcactc atctattatc cggtagtatt tgtagttgtg
    18481 gttggagttt cagcgttatc caaacggtat tttgaggatt atttcctgtc attgaaaaac
    18541 aaaatcagca ttccgaaagc cgttacagca aaaaaataaa caggcgaaat ttttcaattc
    18601 cgcctgtaat tgtatcagtt atttagctct tttcttaagg tcttccaccc gtattgtcat
    18661 cagcctgcct gcgggtactt ttccacggta agtaattatt cgcattaacc gggcatcggt
    18721 cggcactaac agaggtgctt tgtatcgggg atagaaacgg tcgggtgtgg aattgtcgaa
    18781 actggtgtaa atgtccagtc cgctgatttc ggttgtcaat tccacgaagt atttatcttc
    18841 ttttttcgtg acacttacaa tgggatcgta tatggccggc gaatactttg tttcggcata
    18901 atccagccgt tgaaaatgat tttcggtttt gtttacgaaa ttttcccagt ttttcttttc
    18961 cttgggcgac cagaccgatt cggcaatcgc aaaaccacgc ggccatgtca tgtattcaac
    19021 ctgacgaatg ttgtacacct gttctgtcca cagattggcc tgtccaccca gaatataacg
    19081 tgcatcggct tcgggtggaa ccgggtcaaa ctgataggct ttatttaatc tcaacgaact
    19141 atataccggc ggttcggtag aaatatcgcc ctgcatgtag tcaatataag cataggtagt
    19201 cgggctcatc accacataat gcccggaccg cgaagcttca attccatatt ttgtaccgcg
    19261 ccagctcatc aaagccgttg tgggtgtaat accgccttcc agaatttcat cccagcccat
    19321 catctttttt cccctggact gaatgatttt ttccagtctt ttctcaaaat aagactgtac
    19381 ctgcggaatg gttgtcagtc cttcccgctt catcagttcc tgcacctgcg gatttttttg
    19441 ccagaaatta tgcggcgctt catcgcctcc cacatgaata tagggatacg ggaaaagctg
    19501 agccacctca gtcatcactt tatccataaa ttcatagact ctttcattgg ccggacaaag
    19561 cgtattgtcg ataagtgcaa tgggcggtgc accctgcgac cagtccataa agggttcccc
    19621 ggcacgtaat gtgtacttgt cggcaccttc ggtacacgaa agttcgggat aagccaccac
    19681 ggctgccagg ctatggccgg gcacatctat ttcggggata acctgtacaa atcgttcggc
    19741 agcataattc actatatcgc ggatttgatc ctgggtataa aaacctccgt aagtttttgg
    19801 ttcatcggga agcgcaggcg aaaattcacc aaaatttcca accttttcca cccgccaggc
    19861 cccggtttcg gtcagtttag gcagggattt aatttcaatt cgccaacctt catcgtcggt
    19921 aaggtgccag tgaagcatgt tatatttgta gcgcaccatg gcatcaatgt attgttttac
    19981 ttcatctact gtaaaaaaat gacgtgccac atcgagcatc agcccacgcc agccaacacg
    20041 cggataatcg ccaatttcta cagcgggaat tttccacgcc acattaataa cctttgtcgg
    20101 actttctatt tggggtggaa agagctgaat caatgtctgc gcaccgtaaa acaatcctgc
    20161 cggtttattt gcccggatag tgattttttt gggtcgtacc gacaaaatgt atccttcatt
    20221 gccaatcatt ggatcggcta tagcattcaa caccagttgt atgtccgatt taccggatga
    20281 tttcaccgtt actctgcttc cggtagcttt tgataatttt tctttcagaa aattgacagt
    20341 aatatcattc tcactccctg ccggacgaag aatatttacc gattttggca aggtgaaatt
    20401 gccggatttt tcgacagtag tgacaggttc cggaataatg ttgaatttgg cgggtgaact
    20461 ctgactgtac agtataattc cgaacagggc aagagacaag gttaaaaggg agcgtttcat
    20521 tttattgaga ttaagtttat aaaaattatt tgactaagca aacgaatgaa tgtacaaatt
    20581 taggaattta attgctgaaa acaaattcaa aaaatttcct tctgcctctt cgtttgatta
    20641 aacatatttt catgatgtaa gaaataacaa aataacttaa ttaaatattg tacatttgta
    20701 actcatgccc tgttttatgt tatatgaact ggataatata tactttacct tatgaaacaa
    20761 ctacattttt ccaacggtga ccgctttcca ttaatggggc ttggcacctg gctttccaaa
    20821 ccttccgaag tgtacaacgc tgtgattgaa gccatcaggg ccggatatcg ccacatcgat
    20881 tgtgcttaca tttataaaaa cgaaaaggaa ataggcgatg ccctgaagtt tgcattttcc
    20941 acaggacttg ttaccaggga ggaaatgttt ataacctcaa agctctggaa taattttcat
    21001 gcaccgcagg atgtggaacc tgctatccgg aaatcgctga gtgatttgca actggattac
    21061 ctggatttgt atcttattca ctggccactt gcattcaggc aggaacaggc tgcgaatgcc
    21121 ggaggtttga tatcgctcgc tgaacttcct ctcaccacaa cctggaaggc catggaagat
    21181 gtcaaaaaat cgggacttac aaggcacatc ggagtttcca atttcaatat ccccaagcta
    21241 aaaaatctgt tggagaacgc cgaaataaaa ccggaagtaa atcaggtgga acttcatccc
    21301 tacctgcaac agcgtgaact ggtacggttt tgtcaggata acagtatcct ggttacagcc
    21361 tactcgccac tcggcagcca tcatcttatc gataatgaaa attctatcct tcaaaatccg
    21421 acagtcatgg atattgcccg caagcattat tgttcagccg cacaggttat acttgcctgg
    21481 ggtatgcacc gcagcacagc agttattccc aagtcggtta atccacaacg aattcaggac
    21541 aatatgagtg cagaaatcat ccagcttgac gataaagata tggaaagcat aatgagcctt
    21601 gattgtaacc atcggaatgc aaaagcatta tttgccgttt ttccggatgg accttacacg
    21661 ctcaaatcga tatgggatga ataagcctga gataaaaagc tttttctttg tttaattttt
    21721 tgggagaaat tcacagacaa tcaactcaaa gataactgta ctaatgtttt gcaaaccgtt
    21781 ctgccttgta ctgatttcta aattttacca ttacggcaac aacacgttaa actattctgt
    21841 atctgcaacc ttacccgtat taaagttata tcagaaaaaa ttcttatttt tgtccatcaa
    21901 aattagcttt gattaacttg caaatgaccg aacaggaaat acaacatatt cgtgccgatt
    21961 ttcccatact ggaggagaaa atttatggca aacaactcat ttatttcgac aatgccgcca
    22021 ccacgcaaaa gccacaggct gtgctcgaca ggatcaattt cggatataaa cacctcaatg
    22081 ccaacataca tcgcggtgtt cactatctga gccagaaagc taccgaggca cacgaagaag
    22141 cacgccggta tgtagcccgc tacataaact ctgcttcgtc ggatgaggta attttcacgc
    22201 gcggaactac cgaagctatc aatctggtgg cattttcatt tggtgagtat tgctgtcagg
    22261 caggtgatga aatcattatt tcggtcatgg agcatcactc caacatcgtt ccctggcaga
    22321 tgctctgcga gcgaaaaggg atgaagctcc gcataatgcc gatgaacgat aaaggggagc
    22381 tgaaactgga tgaatacgag agattaatca atgagcacac ccgcctggtt gccgttactc
    22441 aggtatcgaa tgtactcggt acggtcaatc cggttaaaga gatgatcagt atagcacatc
    22501 agaaaaatat ccccgtactt attgacggtg cacaagctgt gccgcatatc agagtagatg
    22561 ttcaggagtt ggatgccgac ttttatgttt tctcgggaca caaaatctat gcacccaccg
    22621 gcattggcgt gctttatggc aaacgtaagt ggctcgagcg catcccaccc tatcagggcg
    22681 gcggtgaaat gatccaaact gtaagatttg aaaaaaccac ctacaacgag cttccattta
    22741 aattcgaagc cggtaccccc gactatatcg gctctacggc acttgccgaa gcattgaaat
    22801 atgtggaaac aattggcata gataaaattt cagcctatga aaacgaactg accacttatg
    22861 ctaccgctaa actgatgaag attgatggaa tgcggatatt cggaacatct gagcataaaa
    22921 gcgcagtcat atcgtttctg gttgggaata ttcatcccta cgatatcggc atgctgcttg
    22981 acaaactggg aatagccgtg agaaccggtc atcactgcgc tcagccgctg attgagcaac
    23041 tcggaatacc cggaacggtg cgggcttcct tctctttcta caacacaaaa gaggaagtgg
    23101 atacatttat tgcagcgcta aagcgggtgg tgggaatgtt gggttaacac tacggccccc
    23161 taacccccta aagggggaat ttagtgcatt caatgagcga aagtgcaata agcaaacttt
    23221 taaatatgat ttttctaaac tcaaatacat ataatctata aactatgctc aagattcccc
    23281 cttcaggggg ttagggggca gcctatttta catgacaatc aacgaaattc aagaccaaat
    23341 aatagaagaa ttcagccagt ttgacgactg gatggacaaa tatgccctgc tcattgacct
    23401 gggcaatacc ctgcaaccgc tggatgacaa atacaaaatt cctcaaaata taatagcagg
    23461 gtgtcaaagc cgcgtgtggt tgaatgccaa tctggttgag gggaaagtga tttatgaagg
    23521 agaaagcgat gccgtactcg ttaaaggcat cgtatccatg cttattcagg tactttcagg
    23581 tcataccccc gaagaaattc tggatacaga cctctacttc atcgaaaaga taggccttcg
    23641 ggaacacctc tcacccaccc gctccaacgg actgctggcc atggttaaac aaatgaaact
    23701 atacgcactg gctttcaaat ccagcaagac ataaaatcac tcacagttga tgccattttt
    23761 caatttattc cttcgggcaa gataattcaa agtctaaatc accgtcattt tttgcataaa
    23821 tttccattaa ttttaggcaa ttattgggag tgtatgtgaa ttatatttag taaatttgcg
    23881 gttcatttga atactgaatt ttttccgtat tcatttgtca tctctcatca taataaactt
    23941 tataattaaa tattattccc tatgaaagta actcatatcg agcatttagg cattgccgta
    24001 aatagcatcg aagaagcatt accattttat gaaaacatat tgggtctgaa atgttatgcc
    24061 gtagaagaag tggccgacca aaaagtaaaa actgctttcc tgaaggttgg acagacaaaa
    24121 attgaactgc tggaatcaac cgaccccgaa ggagctgtag gtaaatttat cgaaaaaaga
    24181 ggcgaaggaa ttcatcacct ggcatttgct gtagaaggac tggccgatgc gctgaaagaa
    24241 gcagaagaaa aaggcatccg actgatagac gctaatccgc gcaaaggagc tgaaggactg
    24301 aacattgcat ttcttcaccc aaaatcaacc atcggtgtac tcaccgaact gtgcgaaaat
    24361 ccggataaaa ctccgggaag ttcctgctgt taattgaata atcaataaaa taaaacaaaa
    24421 taataaaaca aaaaaaaaca catgaaaaac caggaaaagg tacaggacct tattgataaa
    24481 cgtgtgcagg ccaaattggg tggaggcgaa aaacgcattg actcacaaca caaaaaaggc
    24541 aaactgacag cacgtgaacg tattgatatg ttgctcgacg atggaagttt cgaagaattt
    24601 gacatgttcg taactcaccg cagcactaat ttcggtctgg acaaggaaaa atacatgggc
    24661 gacggagtgg taaccggtca cggaacaatc gacggacgca tcgtatttat ttttgcacag
    24721 gatttcaccg tattcggagg atcgctatcc gaaaccatgg cacaaaaaat atgtaaagtg
    24781 atggatatgg ccatgaaaat gggtgctccg gttattggca tcaacgattc gggtggcgca
    24841 cgtattcagg aaggtgtaac ttcgctggcc ggttatgccg aaattttcca gcgtaatatt
    24901 cttgcttcgg gtgttattcc ccaaatctcg gccatctatg gtccatgcgc aggtggtgcc
    24961 gtatattcac cggctctcac cgactttatc ctgatgactg aagacaacag ctacatgttc
    25021 gttaccgggc ccaaagttgt aaaatcggtt acaggcgaag atattacaac cgaagatttg
    25081 ggtggagccg atgttcatgc ccgcaaatcg ggtgtcactc atttcaaact ggtggacgaa
    25141 aaggaaggta ccatgctgat ccgcaaactg atctcttaca tgcctcaaaa caacctggaa
    25201 gaaccacctt tgtacccgac caccgacccg atcgaccggt tggaagattc gctcaacagc
    25261 attatccccg ataatccaaa tcatccttat gacatgaaag atattattca tgccattgca
    25321 gatgacggcg aattcctcga agttcaccgc cactatgccc gtaatatcat cgtaggtttt
    25381 gcccgcttca acggagcatc aaccggtatt gtagccaacc agcccaattt cctggcaggt
    25441 gtactcgatg tggatgcatc gcgtaaggct gcccgttttg tccgcttctg cgatgctttc
    25501 aatatcccca ttcttacact ggttgacgta ccgggcttcc ttcccggttc tggtcaggaa
    25561 tacgccggta tcatcattca tggagctaaa cttctgtttg cttatggaga agctaccgta
    25621 ccgaaagtta ccgttactat ccgcaaatcg tatggcggtg cgcatgatgt gatgagcagc
    25681 aagcaacttc gcggcgactt caattacgcc tggccaacag ccgaaatagc cgtaatgggt
    25741 gcagcaggag ctatcgaagt gctcgaagga cgcaatattg cagccataac cgatcctgaa
    25801 gaaagagcca actatgtaac tcaaaaagaa cttgaataca aagaaaaatt cgcaaatcct
    25861 tacgaggctg ctgcctatgg ttacattgac gatatcatcg agccccgcaa tacccgtttc
    25921 cgcgtgatcc gcgctttcga agctttacaa accaagaaag taataaatcc gccacgtaag
    25981 cactcaaata tgcctttgta gtcggatgta ttcattaaca attaaataaa acataaaatg
    26041 ttaatagcaa taatagcggt actggcagtg gctctggctg taattcttta tcttttttta
    26101 cagaaaaata aggctgtgaa agaaattgca gcagagaaac agtcattcaa agcacagacg
    26161 gccggtgaaa atacggtaaa atcagaagtt gtgcctggca acgatgaaga agtggctgcc
    26221 tttctggcat tgcatcttta tctgacacac aatgtgcacg atgtggaaag taatgtgatt
    26281 accatcgaac gaattcagcg gcgttattct ccctggagtt ccaagattta cagcatcaac
    26341 aatttccaac ggtaattcat taatgtgaaa ttaaaaaata aaataattca ccatgagaaa
    26401 atataaattt acaatcaacg aaaaagagta taatgttgta gtgaaaggca tcgatgatga
    26461 aatagccgaa ctggaagtca acggaaatcc ttatgaggta aaaatccatc agctggaaca
    26521 aaaagttccg aaaactccga ttttggttcg caaagaagta caaaacaaac cgggcgaaaa
    26581 caaagtggcc gaaaaactgg ctccaatgcc aattaccgcc aaaccaagtt ccaaaactat
    26641 caatgcaccg cttccgggta gtgtcataaa gattgttgtt gaagttggat ctgttatcaa
    26701 agaaggtgac gtactgatgg taatggaatc catgaaaatg gaaaacaata tcctggccga
    26761 aaaaagcggt actatcgtga aagtatgcgt tgcaccgggt gcttctgtta tgcaggatga
    26821 agctttattt gaaattcaat aaagagcaac tgtacttata aaatacaaaa acaactaata
    26881 atccggctga aattcttcat tgatttcagc cggatttttt ttaaaacaga tgaactgatg
    26941 gagatgtaca ttatttttca atggattttt atttttacag agcggaaatc gaaatcatta
    27001 atcaggtatt atcatcaatc attacctgac aattaattca tttttcgtac atttgtatca
    27061 cattgaaatt tataaaccat ggagaacacc accgactttc atgattatgc acgtcagttt
    27121 gccgaactta ccaactgcaa tatattcctc accggaaagg ctggtacggg taaaaccacc
    27181 ttcctgcacg agctccgcaa aaataccggt aaacaaattg ccgtagtggc tcctacgggt
    27241 gtggcagcca tcaatgcagg tggtaccacc atccattcat tcttccagct gcccttcacc
    27301 cctttcattc ccaccgccga aggacgcaaa gacctgattg gcaaactgaa aatgcaaagt
    27361 caccggcgta aggtaatccg cgaactggaa ttgcttgtaa ttgatgaaat tagtatggtg
    27421 agggccgatt tgctcgatgc cgtagatacg ctgctacgcc acatccggta tcgtaacaat
    27481 gagcctttcg gaggcgtaca gatgattttt atcggtgata tgtaccaact ttcacccgtg
    27541 gcacaggccg aagaatggcg attgctgtca ccttattacg aaggcattta tttttttcac
    27601 agccgcgtca tgcagcagaa acctccggtt tatatcgagt ttgataagat atttcgtcag
    27661 aaagatatgt cgttcatcac cctgcttagc gaaataagga acaacaactt atcggcagcc
    27721 ggactgacca tgctgcaaag cagatacaat ccgctgtttg tacctcccaa ggacgatacc
    27781 tatattatcc tgactactca caactataaa gccgaccgca tcaatgccga agaattggct
    27841 gcgttgaaag tcaggtcacg atacttcaat gcagaggtga aaggtgattt tcccgaaaaa
    27901 ctattcccga tagaggaaaa actggaactg aaaacaggtg ccaaagtgat gtttattaaa
    27961 aatgacaccg aaacgccccg aagatttttc aatggaaaaa ttggtgtcat cacggcattg
    28021 ggggatgaga ctataacggt gaaatgtccg ggcgacgaca gggaaataga agtgagcccc
    28081 atgacctggc acaacgtgcg ctacaaggct aacgaaacca ccctgcaaat cgacgaagaa
    28141 gtacttggca catttactca gtttccgcta cgcctggcct gggccatcac cattcacaaa
    28201 agccagggac tgacctttga aaaagcagta atcgacgccg gtgcggcatt tgctcccggt
    28261 caggtgtatg tggcgctgag ccgctgccgc tcactggaag gaatggtgct tctcagcccc
    28321 attaaccagt attcactaca gaatgacgaa caaatcgttc gcttttcgga tcaaacaaac
    28381 accattgacc aactcaccac acagttggaa gtttccaaac gcaattatca ggaaaccctg
    28441 cttctcggaa tattcgattt caaacccgtt gttgccacgg ctaaccgctg gctcaaccgg
    28501 gctaaggatg tggcaagttc ttttaataaa gacacgctgc cttttatcca aaacattatc
    28561 aaacaaacgg aagccctgca ggatgtagct tcccggtttc agtcgcaact gatccagatt
    28621 aatggcacca cacccttcaa tcaggaatta ctcaccagca ggcttcaagc ggctaacggt
    28681 tacttttcgg agcacctgag taacctgagc gagacactca aacaaactac cgccaccacc
    28741 gacaaccgcc aaaatgccaa tgagtacgac gattttctgg aagatatcta tacgtatgcc
    28801 gaacaaaaga aacatcttat tcagggaata caaaatggat tcagcccgga gaaatatttt
    28861 gacctgaagc gtagttttga actgcctaaa ttcagtcata gctcttattc caaaaccaat
    28921 acaaatcagc gaattagttc cagacatcct caattgctca attatctgtt tcaggcacgc
    28981 aatgaaatca gcgataattc gggattaccc atttacctga tagcaacggt gaaatcgcta
    29041 acagaaatgg cggattatat gcctctgaac gaaaaagatt tgttgaaaat atcgggattt
    29101 ggcaaaccga aggtggataa gtatggtgaa cagtttctga atatcattcg ccggtattgt
    29161 gacgacaata atctgtcgac cgaaatgagc ctgttcgaag agaaagcaaa aaaatcttcg
    29221 gcaaaaaaag agaaaaaacc gaaaacagat accgtacgtg ttacgctcca attttatctg
    29281 gatggcatga gcatagctca gatagccaaa gaacgaagtc tgaccgaagg aacgatatca
    29341 aagcatctgg ccgatctggt ggaaaaaggc gtgctgaaag tggagcgatt tatcggtcat
    29401 aacaaactgc agcaggcagt tggtttgctt gaaaaaggca atctgagtgg ttcggtgtac
    29461 gaattattga gcaacatcat gtcgcagcag gaaattataa taatgcttgg ctggatacgg
    29521 ggaggaaaaa aagagggaaa actataactt ctctgtgtct gcaatccgat gatttcgggt
    29581 tgcttacttc ctgactttgg tgccgttctg attgatgtaa tctttccagc tcttatattc
    29641 actgtcaatg gatggttttc ccgattgcag aaaatggcag tatgcagctg ccagcccgtc
    29701 ggtggcatcc agttggggca gcatatcttc ctgcgaaatg cgcaggtatc gccgcagcat
    29761 atcggccacc tgttccttgg cggcacgacc gttgccggta attgccatct taattttcag
    29821 cggtgcgtat tcactcaccg gcagatcgcg gtacaaagct gctgcaatgg ctgcaccctg
    29881 tgcacgtccg agcttgagca tcgattgtac gtttttacca aaaaaaggtg cttcgatggc
    29941 cagacaatcc ggatgaaatt catcaacaag tgagagggtt ctggcaaata tccgttgtaa
    30001 tttttgataa tggtcggcat atttctttag ctccagcaca cccatggcca gcatttcggc
    30061 cttattatcc tttacccgga taattccgta acccatcacg gtagtaccgg ggtcaatgcc
    30121 aagtataatc tggtcctttg ccattacagt taaaacagct ctaaaactgt ggaaaatgaa
    30181 agaacttcgg taccaaaccg cgaggctaag tcattttgaa ctaattcagc atcattttct
    30241 tcccacaatt gcagcgtaat caggtcgttg accagaaact gtaccgaaac agaaaccccc
    30301 tcctgctcct tgtcctcagt aagtacttta gctatttgag ggcttgtaaa agaaccggtg
    30361 ccgagcatgt tgggtatgtg cacttctgtg agccacttga accagggatt aaactggcga
    30421 tcggccacca ggtatgtggt gttgtatata aacttagcca tttcgtaaaa ttttttacaa
    30481 aaatacgaat ttatttccaa aaatgcttat ctttgcacct cgaaaggcaa atgggggttt
    30541 agctcatctg gctagagcgc aacactggca gtgttgaggt gaccggttcg agtccggtaa
    30601 tctccacaaa gaaaaatcta tatcgctgtt attcagtaat atagattttc ttcttttaaa
    30661 tcctgcgcca tttacagcga aataaaaatc actcagcaag tatttttgca tttgccagtg
    30721 gcttttttac accactcggc atcagttgga aaagttacat ttactcattg aatttacaac
    30781 acgaatgaat tcgggaaatt aattaaatgt attatccggt tagaaattta acgacagacc
    30841 aaccgtaaaa gtctgaactt cgggtccttt atcctttttg aacagggtgg taggcgtgta
    30901 ttttgcaaat acccctaagt catcccagcc catttcaacc aaataactca gagaaaaagg
    30961 attgacatta taatcgttac cttccacctt tttgactttg gtgttatcat cggttttgta
    31021 tttcacttta tacgaactaa atgtatttat cccgaacaga gctcctccca tgatataaaa
    31081 ttccgacttt ctgacaggat gaatttcaag atacagcggc atattcagct cgaaaattcg
    31141 caaacgtgac gaataatagg aaattccttc gggagcggct tgggggattg taattccgtt
    31201 gttatttacc aggtggctgt tgttacccaa atgaagattt ctccatatca ttccaatccc
    31261 ggtggttatt ccaacatatt ccttaattac gggcagtgaa taacctatgg gattaaaggt
    31321 gaattcgttc gagaaaacga ccttcattcc tcctgcatcg ttgaaattag agaaattttc
    31381 agtagtattt agtgcaccaa atcctatacc gataaagtga ccggaaaatt ttgacgaccg
    31441 ttttcttttg tcaaaaggaa attcaaatcc gaactgtgtt tcaacagaat atttctcaaa
    31501 gctgttttca tcggtaaaaa taccttcata aatcttttta tattcggcgg tatccgtttt
    31561 atatacctga acctttacct gatcgacact gtcggtaacg tgtatttgtt tctggttaaa
    31621 aattaaggtt gtgtccttaa cggcttgtga ttgagcattg acagtaatgg atgctgtcag
    31681 gattaatgct gatagaatta aaatttgcgt cttcatacgt ttcattattt tttctttaat
    31741 attaatttca ttatttcttc cgaatctttt tccgattcga tgtatatcag ggtcatttta
    31801 agttccggaa cagacttttc gttgaaaagt atcagcctgt tgtattttcc ttttttgggt
    31861 aattgcaggt aaacggaact tattttgccg ttggtgacta cctgttttac tttttttgca
    31921 ccattctgat ctgtttgcag gcatccgcgg gtaaaatcac cggcttcttt attgttgctg
    31981 ataacaatac tcttgtaaaa cgtgaaatcg tatccttcga gcatctcttt gctcatttct
    32041 accattacag aacctttctg tttcccgtat ttatcgaata tcctgttaat atcgagatcg
    32101 ctctgcgctt taatttccaa tccacatcct gcaatgaaca tcacgacaag gatcaggtat
    32161 ttacacttac tattcatttt catgattata tattttttgt tataattcaa tttccgaaaa
    32221 ggcatccaat tgggttttga ttgtttcatc attctttaag ttgtccagtc cgtcctccac
    32281 ttcgttattg tccgacacat aatttattgt cgtttgagcc agtgaaatga tctcctgtgt
    32341 gttggttatt ctcactccgt tgatgtatgc atagctcgtt tttcctgagg cctgaaagat
    32401 tgaaaccggg attatcactc ctgcggcaat aactgccgct gccgcccatc tcaggtaagg
    32461 ttgaagcctg aagtgctttc gggctttttt actttcataa tatccgaaaa ttgctttctc
    32521 cgcttcgaac tgcggaggca cattgctttg tgccagaaaa gttctcagtt gattttcttc
    32581 ggctaccgtg gtttcacctt catagtattt ctcaatcagc ttttcagctt catcaactgt
    32641 aataatattt cgtctgttta gcttcatctc tgtgtaagtt taatgtattc ttctctgact
    32701 ttttttctcg ctctggataa attcattctt acagcttcat agcttgttcc ggtaatttca
    32761 gcgatttcat caatttcgta ttcctctaca tctttcatgg tgatgatttg ttgctgcagc
    32821 tgcggtaatg tcccgattat gtgtttcaga gtttgttccg tattttttct ttccagttgc
    32881 aggtgaggat tgtcccttgc agacactgca taaaggttat cgattgtttc agtttctttt
    32941 ttcagtttca gtttatcaag gcagtggttt tttgttaccg ttgtagcaaa agcagccgga
    33001 ttatcgcagg taatcagcgt gtcccgctta ctccacaatt tgagaagcac ctcctgcacc
    33061 gcgtcttccg catcctcact gtcatcaagc attttcagcg attgtctgaa aagcttatga
    33121 cgcaacggca gtatttcttt cttgaacagt tctgaactca tttacctaat tgacgaataa
    33181 acatct
//
LOCUS       DAOPZK010000004        31700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1349_length_31700_cov_21.748428, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000004 DAOPZK010000000
VERSION     DAOPZK010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 31700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31700
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1349_length_31700_cov_21.748428"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKH68_00455"
     CDS             complement(<1..778)
                     /locus_tag="PKH68_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNX11435.1"
                     /translation="MKLIYKSTFIVAFAANIITGGVYAQNNNEEVVVNSPFSYEASYT
                     GDLVSNFKGGIKTGTAYLGLANLKAGFDTEKARWWKGGQLFVNVGNTHGGEPSATLVG
                     DFQGISNIEAGDLTFMYELWYKQSIGKLDFTVGLQDLNANFAVSENGALFTNSSFGIH
                     SSIADNIPSPIFPLTALGVTFQYKISDTFNWQVAIFDGTPDDFENNPYNINWKLSKDQ
                     GYLAVSEFQINKSLIKGRNASYKLGGYYHQHNDEIDREQKN"
     gene            complement(952..1566)
                     /locus_tag="PKH68_00460"
     CDS             complement(952..1566)
                     /locus_tag="PKH68_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNX11436.1"
                     /translation="MNSNQLIIAIVEPSVIIRTGISAVLKRVPGYRIQPVEIITIDSM
                     NHYLNMHKPDILIVNPSFWGYFDIAKIKAETDNAKLKCIALVYSAIDENLLRNYDDRL
                     SIYDTVDQLKEKLDKLFNTSLTENADEQNTLSVREKEIITGVVRGLTNKEIAKSLFLS
                     THTVITHRRNIARKLEIHSTAGLTIYAIMNKLIELDDIKQNPLI"
     gene            complement(1563..2270)
                     /locus_tag="PKH68_00465"
     CDS             complement(1563..2270)
                     /locus_tag="PKH68_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNX11437.1"
                     /translation="MSKQHKFSASDKMSDLINENHSLLLVISRFGLSLGFGDQTVKEV
                     CIANGIDCNTFLAVVNFLTEDNIEVDYDYGDISIVSVINYLKNAHSYFLEFKLPQIRN
                     KLIEAVNNQDQSIPYSSIFLKFFDEYVMEVRKHMEYEDKVTFPYVLKLLNGEKVSNYT
                     IAVFRNHHNDIESKLVELKNILIKYYPDNGNSYLLTEVLFDIFSCEMDLATHNQVEDF
                     LFIPEVEAVERKIKMKL"
     gene            complement(2458..3564)
                     /gene="meaB"
                     /locus_tag="PKH68_00470"
     CDS             complement(2458..3564)
                     /gene="meaB"
                     /locus_tag="PKH68_00470"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNX11438.1"
                     /translation="MYNPHHQHPENDPSYKGLSVNEGVQDAPIINPYAKPRRTRKHFT
                     PAEYVEGILKGDISVLGQAVTLVESSLPHHQEIAQEVIEKCLPYAGKSVRIGITGVPG
                     AGKSTFIEALGMHLIHAGSKLAVLAIDPSSERTKGSILGDKTRMEELSVAKNAFIRPS
                     PSAGSLGGVARKTRESIVLCEAAGFDVVFVETVGVGQSETAVHSMVDFFLLIQLAGTG
                     DELQGIKRGIMEMADGIAINKCDGTNIEKSQLARRQYLNALHLFPLPESGWKPEVITC
                     SAIERTGIAEVWQMVQNYVDFVNGNGYLDRKRTDQSKYWMYETINEQLKSNFYNNEKI
                     QALLQVAENEVLSDKISSFVAARQLLDAYREMNW"
     gene            complement(3680..4768)
                     /locus_tag="PKH68_00475"
     CDS             complement(3680..4768)
                     /locus_tag="PKH68_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNX11439.1"
                     /translation="MNKKFGVLLIVSVLVTLTTWAQKPVITFDEKTFDFGTINETDGK
                     ATHVFEFTNSGNATLLVQRVNASCGCTTPEWTQTPVEPGKKGEITVTYNPAGRPGAFT
                     KTITVYSNASNEVESLLIKGTVNSASSPAVNNYPIQIGDLKVKTKTVLLNNVSKGSTK
                     TNTLLIQNGSKSKMTVSLADLPVYLKAEAKPAVLNPNEEGRIEFTFDSKKCAEWGPVF
                     DNIYLVLDGKKVADELHKITVNANVVDDFSKMTIEEKQQSPILEIKSPNLLFGSIKQG
                     SKVRGQAIIKNNGQKPLEIRRVINNNSNVLVHPMRLSIKGGKTGELKIDIDTKTLPQG
                     DYKKSFTIQTNDPENSFLIYTLSWTVKK"
     gene            4966..6147
                     /locus_tag="PKH68_00480"
     CDS             4966..6147
                     /locus_tag="PKH68_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNX11440.1"
                     /translation="MNEDFKLLSDSIFNYTSTCYRHLHAHPELSFQEYETSTFIQKQL
                     TDMQIPFRSNIAGTGILGRIEGRNPQKKIIALRADMDALPVQEAVNIPWKSKNDNVMH
                     ACGHDAHVACLLGAAKILKQVCDEFEGTILLIFQPGEEKAPGGASLMLQNGLFDEYKP
                     EAIIAQHVSIDYPTGTMAFLSGTIMASADEIHLTIRGKGGHGALPHLCNDTVLAASQT
                     VVALQQVRSRLCHPLTPMVLTFGRLIAEGATNIIPSEVHLSGTFRTVDERWRKEALEH
                     IRRIITETCSAHSCTVEIDIPDGYPCVVNDATLTEKIKGFAGEYAGEENIDKLELRLT
                     SEDFGFFTQQYPCSFYRFGVKGDTNQDMGGLHSPTFCIDEKALLTGAGGLAYIARRMM
                     D"
     gene            complement(6151..6684)
                     /locus_tag="PKH68_00485"
     CDS             complement(6151..6684)
                     /locus_tag="PKH68_00485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX11441.1"
                     /translation="MEIIQIKSANDEYIQKLLPLYREAFPENQRHTDDDFIALLGREP
                     DFFCNAVLMNNSFVGFFNYWNFGWFLYAEHFAIEEAIRGNKLGEKVMNMMKPTQNIPL
                     VFEVELPDDEMAARRIEFYRRLGYEIVPVDYLQPPYNKQGYYQPLYLMTDQVEFVVAN
                     YEKIKSTIYRRVYHVME"
     gene            complement(7006..7599)
                     /locus_tag="PKH68_00490"
     CDS             complement(7006..7599)
                     /locus_tag="PKH68_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX11442.1"
                     /translation="MKIEINKVVNAEYELYVDGENGELELMERATPEQPLNFIYGVGM
                     MLPKFESNIAGLEQGDAFDFTISNEDAYGEYDDDAVVELERSVFEIDGKLDEEMVFEG
                     GVVPLMDNEGNRLQAQVVSVSDTHVTVDLNHPLAGETLHFKGKVLEVRDATDEELKSL
                     LGGGGCGCGCGCDDNGCGDDGCGCGDDDSKGCGCGCN"
     gene            complement(7665..10370)
                     /locus_tag="PKH68_00495"
     CDS             complement(7665..10370)
                     /locus_tag="PKH68_00495"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNX11443.1"
                     /translation="MACFTYLQNAYGQKATAMSQPQNAVQLPDSLKMAASKDTAQITK
                     KLSPNQIDAEIAYTATDSIVFMGDGTGFLYGKTNLSYKKINLKEADYVRISMDSTTIF
                     ARGVPDSLGVMQGKPIINDGDQEYNANELTYNLRTRKGFIRQAVTQQGEGYIISDKTK
                     KTEDDVLCMQQGMYTTCDNHEHPHFYLSLSKAKVKPGSYIITGPAHMVLLDIPLPVAV
                     PFGFFPFNKNYSSGVEMPSFDTDITRGYGLVNGGYYFAINDYADLSIKGDVYTRGTWG
                     LNLSSNYLKRYKFNGGVSISYREDVIGEKDMPDYQKNPNLSIQWSHSQNAKVNPFSTF
                     SASVNFTTSGYNRSNINSYYTPANADNTKSSSVSFSRRFPNNPFSFTANMSVTQRTKD
                     STINLMLPNVAISMSRLFPLKRKNAVGKERWYEKISMSYSGTIVNSIQTKENKLLKSS
                     FARDWNNSAQHSIPVSASFNVLKYITFTPSFNYREKWFLTSQRKNWDVVNQKEVTTTV
                     TGFNRVWDYNLSMSANTTLYGMYTPLKAIFGDKIEKIRHMAKPNASFSLTPDFGDLKY
                     GYWDTYIKSVIKDGDPTKITSEEIFYSRFPSGAPGRGKSNSLSFSLDNNLEMKVKNDK
                     DTTGLQPTKIISLIDNFSLNSAYNFAADSMRLSNISANLRIKFGKAYTLSLSGAFDPY
                     MYASPDGKAIRRVNQLRWNHGKFPRFLGTSTSYSYTLSNETFKRKDKKSKPKGDDQAD
                     DSQLNEGMNRDNNNMQNKDNKSNESGAEVKTDAEGYEKVEIPWSLSFNYFIGFGNSSE
                     FNYEKLEYKQEIKHNVGITGNIKLTQNWQFSGTTSYDFNAKQMSYTSFNITRNLHCWT
                     LTGNVVPFGPYKTYNFRIGVNSSMLQDLKYEKQGNRSSSNNISWY"
     gene            10503..11834
                     /locus_tag="PKH68_00500"
     CDS             10503..11834
                     /locus_tag="PKH68_00500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNX11444.1"
                     /translation="MNNLIFYNLLNASKQDKITTFAFSFLRIFHSSFFYSKTMKYFRY
                     ITLVILLFLTCTVSSLYAQKKFTVVIDAGHGGHDSGAVGRYSKEKNINLAVSLALGSK
                     IEANYPDVKVVYTRDKDFFLTLQERADIVNQNNADIFFCIHTNANNSSTAFGAETYTL
                     GLHKSQSNLDVAMRENSVITLEDDYKTKYQGFDPGAPDTYIMFEFMQDKYIDKSIELA
                     SAIQNQFVRASRYDRGVRQAGFWVLHKSACPSVLVELGFISNRAEEDYLNTNEGREEM
                     ANSIFKAFETYKRDHDKKSGFKVVPLKKEDTPAPKKEDVKPVKVDNPVIAANVPTTDT
                     TKKTLKNEVVKVNKPDVPVADVKQNNQVIFKIQLFAVEKKLRKGSSEFKGLNDTDFYV
                     EKNLYKYTYGEETSFSEINKLRKSISKKFPNAYVIAFRNGEKIAIKDAMKK"
     gene            11868..12776
                     /locus_tag="PKH68_00505"
     CDS             11868..12776
                     /locus_tag="PKH68_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNX11445.1"
                     /translation="MKKNIFTREVKVGLMVIVAIFILYFGLNFLKGIDIFQKTAIYYG
                     QYEDIGGLVPSAPVYIKGYKAGQVEEVKYDFSKKESFIVKISVNSDIKLPVGTTMQIA
                     DEGLMGGKVIQLVYAPVTASQALYKQNDMLPTTVSTGLMASVAGDLMPKIDKVATQAD
                     SLLYSVRLLIERKEISNSLGSIEKTTADLAASSAQLKGMLNNQLPSILNDVNVVTGDF
                     KQVSGKLKNIDFAQTFASVDYTLKNLQNMTNKMSSNEGSLGLLMNDKTLYINLSNTAG
                     AADKLLIDVKENPKRYVNFSVFGNKK"
     gene            13205..14602
                     /gene="dnaA"
                     /locus_tag="PKH68_00510"
     CDS             13205..14602
                     /gene="dnaA"
                     /locus_tag="PKH68_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNX11446.1"
                     /translation="MSYHYELWNSCLNIIKDNVNEASFNTWFAPIVPVKFENKIFVLQ
                     VPSQFFVEYIEEKYIDLLRMTLTRVAGNGTRLEYRVLIDKTSGTGTQIPSTAEHSRPN
                     TTNYNAQQNTIVSPYKRMQLPEIDPQLNPSYNFNNLIEGRSNKLARTAGISIANDPGK
                     TVFNPLFMYGKSGVGKTHLANAIGVMTKQLQPHKRVLYVSANTFQIQYTDAVRNNTQN
                     DFLNFYQTIDVLIMDDIQEFAGKTATQNTFFHIFNHLHQLGKQLVLTADRSPLEMAGL
                     EQRLLTRFKWGLSAEIEKPDFELRQSILKSKIYRDGLEIPEVVVDYIAENVVDNVRDL
                     EGVLISLLAHSTLTNMPIDIRLAEKIIGRIVNMTPKVTTIEQIREVVCDYFKLSVDAI
                     STKSRKLEVVQARQIAMYLSKNMTKNSLSMIGNCIGQRDHATVLHACKKVVDLMDTDK
                     HFRKNVEEIEDRLRK"
     gene            14743..15303
                     /locus_tag="PKH68_00515"
     CDS             14743..15303
                     /locus_tag="PKH68_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11447.1"
                     /translation="MIFKFTIISDEKDDFLRIIEIDSEATFLDLHNAILESVDYEKNQ
                     MTSFFLCNDDWEKGQEITLVEMESSSEYDNLVMEDTVLDELLMDEKQKLIFVFDMISD
                     RAFFMELTEMIPGKDIDEAVCTTSTGSAPVQIAEETDFATPQKMQIPSDENFYGDEEF
                     EIDELDEEGFGDLNFDENSLFTDDIR"
     gene            15375..>16240
                     /gene="miaA"
                     /locus_tag="PKH68_00520"
     CDS             15375..>16240
                     /gene="miaA"
                     /locus_tag="PKH68_00520"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNX11448.1"
                     /translation="MHTLLVITGPTGVGKTAVSLLLAEKFGCPIVSADSRQFYRELKI
                     GTAAPTDDELEQVKHYFIGTHSIFDEYNAGQYEEEAISLLDELFEKHEVVLLTGGSMM
                     YVDAVCNGLDNIPTVTADVRQFWQKQFASHGLEFIQHELKRLDEKHYAEVDLKNPKRI
                     IHALEICSMTGKPYSDLRTGKRKIRNFNILKIGLNRPRPELYERINLRVDKMMHDGLL
                     EEATRYYPYRHLNTLNTVGYKEIFEYLNGLCDLDTAINLIKQDSRRYAKRQLTWFNRD
                     KEINWFHPEEVE"
     assembly_gap    16241..16340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16341..17063
                     /gene="murB"
                     /locus_tag="PKH68_00525"
     CDS             <16341..17063
                     /gene="murB"
                     /locus_tag="PKH68_00525"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNX11449.1"
                     /translation="FVAYCVSNNYYGVENLSLIPGEVGASAVQNIGAYGMEVKDVIET
                     VDTIHTETLNERIFTNEECRYGYRKSIFKEELRGKYIITNVTFRLKKQPDYQLDYQHL
                     REAVLQRGEINLANIRSTVISIRESKLPDPSITGNAGSFFMNPVIPKEKFEELQVSYP
                     TMPHYPASETEVKVPAGWLIEQCGWKGRSVGNVGVHSRQALVIINLGGATGPEVAGLA
                     AQIRENVFQRFGIDITPEVNYI"
     gene            complement(17054..17548)
                     /locus_tag="PKH68_00530"
     CDS             complement(17054..17548)
                     /locus_tag="PKH68_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNX11450.1"
                     /translation="MQSTHNIEIERKFLVAGDFKPFVSAGFRITQGYLCSAPDRTVRI
                     RVKGDRGFITIKGKSGDNGFSRFEWEKEITFDDATQLLALCGSGVIDKIRYEVPFEGK
                     VFEVDVFEGENEGLVMAELELQSQEEQFTKPAWLGREVTGDRKYYNSYLSQHPFREWA
                     NNQI"
     gene            complement(17572..20445)
                     /locus_tag="PKH68_00535"
     CDS             complement(17572..20445)
                     /locus_tag="PKH68_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNX11451.1"
                     /translation="MNSFLYRVAKAYYHQYDTDILNYTFVFPNRRAGLFFQKYLSELI
                     DKPVFSPAILTVNDCFFNASQRRVADRTRELFRLYSIYRQVSGSDEPFDTFLYWGEIL
                     LADFNEVDKYRIDARQLFTNVKDLKDLDLFFDVFTEEQKSAIKHFWENFIPIIEGKTK
                     EEFIATWRILLPLYEEFRRQLFAENTATEGMIFREVSEQLKRKEDPDYFSGKKFVFVG
                     FNALNPCEKLLFTELQKRGQADFYWDYEAAELRDSDNQASLFYKENIQNFRSALQLDN
                     ETPDLKNKSWELISMPSAVGQTKEVYRLLNELYPDRSEDKNWIKTAVVLPDESMLVPL
                     LHSLPPSIEKINVTMGFPLNATPVSSLLENIFELQRRMNKRQQFYHHTVSGILNHQYI
                     HLLCSDDAVSIAEMMIRTNAMYVEPEAFNKNELLAAIFTPHTSSVGFVEYLLDILKKL
                     NIAWQHKADAENDFRLECDFLFQYYISLNRLNEVITSVADTAEITIDTLLKLVRQLIG
                     GISIPFEGEPLNGLQIMGMLETRGLDFENLIICSFNEGVFPKKSTSNSFIPYNLRKAF
                     NLPTAEYHDAISAYNFYRLISGARRLIFLYDSRNEGAQTGEVSRYIHQLRYHYGVQFR
                     LINVAYDIRTPSDRTMQVEKTPEVMAKLQPYLSDANDAPALSATSLNSYIDCPLQFYL
                     TQVEQMREPEEVQETIEAGMFGTLLHAVMEGLYKSFEGKTVHKTALEELWTNPLTVDR
                     AIRTAFSENYFMKGKNPDLVLEGNYLLVARVIRKYVIQILKKDAENSPFNYIQSERKE
                     RMRFPIHGGESAVNLKGYIDRVDERQGVTRILDYKTGSGSLEFKSPDDVFKRDMDKRP
                     KFVLQTFLYCLLYGHTNDTEQLVPEILYIRDLFKTNFTTRLIDKSGTNGVGAVDNFED
                     YKSGFISNLTVCLEEIFNPEVPFTQCTNAKTCEYCPYKVICRR"
     gene            20568..21266
                     /locus_tag="PKH68_00540"
     CDS             20568..21266
                     /locus_tag="PKH68_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNX11452.1"
                     /translation="MIGTIVNTATVIVGGCIGLLLKTGMPDRLKTIYFQAVGLFTLAI
                     GIGMVWNMEHILVVVSSLVIGSILGEWWNIEKGTDELSEWTKKKLHIGSEKFSEGLTT
                     SFLLFCIGAMTIMGAIEEGTGGTSRLLMTKSVMDGFSAIILASAFGVGVVFSAIPLLL
                     FQGGLTLAAGFAGDFLSPDMIRALTSIGGILLIGLGINILEIKKLRIMNMLPALVVVV
                     LLMWFVAKFNIQVL"
     gene            21263..21703
                     /locus_tag="PKH68_00545"
     CDS             21263..21703
                     /locus_tag="PKH68_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11453.1"
                     /translation="MNITQAFIWFLIGFLLVNGLKFLHTILGSRMKHKRIKNNEIPVR
                     GYYKQAYFGMLRFQPFYYVIIWLVCSYFYFSGHISGNSFADAMITGISWWALTVVCEM
                     LIWVLVNHTMNLSWKEMYLQSQPWISLTYYAVLVSPLIISLVLH"
     gene            complement(21711..>22711)
                     /locus_tag="PKH68_00550"
     CDS             complement(21711..>22711)
                     /locus_tag="PKH68_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNX11454.1"
                     /translation="KIQPEYVADFVEKNEKMLDKIQPSFFEATMAMAFRYFADCAVDV
                     AVIEVGLGGRLDSTNIVSPALSVITNISFDHTEFLGNTLAQIAAEKAGIIKPETPVVI
                     GEVVPETRPVFIEKAQKHHSPLVFAEENIPVKFIEYSADKMIVETLDNEKLIVGLCGE
                     YQLKNIATVLTAVEELQKLGFAIYREDVKNGLENVIPLTNLQGRWQLVRHKPTVVLDT
                     GHNKGGIEYIVHQLRSQNYEKLRIVIGMVKDKDISGVLSLLPRDAVYYFTNATTERAL
                     PALKLKEKAERLGLNGNAYSAVEQAVKSALKEAGENDFVFVGGSNFVVGEALPVFD"
     assembly_gap    22712..22811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23059..24396
                     /gene="tilS"
                     /locus_tag="PKH68_00555"
     CDS             23059..24396
                     /gene="tilS"
                     /locus_tag="PKH68_00555"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNX11455.1"
                     /translation="MDTLHKVQLFIENNKLLTPQSRLIVGVSGGIDSVVMLNILSRLG
                     YECVVAHCNFHLRGKESDRDEEFVAKLAEKMGLLYKKTDFDTVSYAKRRKISIEMAAR
                     DLRYDWFAGLLTDLNAQAIAVAHHADDSIETMLINSVRGSGLRGLYGIDARNGNVVRP
                     LLGCTREEIEIYARKNKLSHVFDSTNASSDYLRNRFRNDILPALAHINPSVKHTFLEN
                     MNRMRGAWKIYNQKIQEIKAEICSAHGNQMYINIPKLLEQADVTTVLFELLQPYHFNG
                     KVVEDIVNSLKNTSGARFFSDTHCVLKDRNQLIIEEKEVDSDVEYLIEKQTKFINEPI
                     GMEFRQFAAGKDFQVSRQPDKIHIDADKVSFPLTLRPWRKGDVFYPFGMTGKKKVSDF
                     FIDEKINRFSKEDAWLLLSGKEIVWIAGLRLDNRFRITPETKQILEVSLKSNN"
     gene            24580..>25403
                     /locus_tag="PKH68_00560"
     CDS             24580..>25403
                     /locus_tag="PKH68_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNX11456.1"
                     /translation="MTNYNILQLNAKELSELKEIAAELGLKVSNSATKEKLVYDILDQ
                     QAVANAQQKSAAAEDQDANRKKRMRIIQKKGPEQKVFSTNPTVTPATAETKPQPTVQP
                     TSSPKQVQKTPAQKLPKAPQPASTEAILTEEPAQKQEQKKKNPRNSKKTAVTKTSPTE
                     TTAVQPTVKEDAPKQAPIADQKVMPLKKVVLLPEVLEETPEMELPQEPQVQTETTDDT
                     AVAAAKTEQVQNQNTNKGNQPVHQGTQPNGNRVKFNPRQGEKEKTYDFEEILEGTGT"
     assembly_gap    25404..25503
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25504..26349
                     /gene="rho"
                     /locus_tag="PKH68_00565"
     CDS             <25504..26349
                     /gene="rho"
                     /locus_tag="PKH68_00565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005652729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNX11457.1"
                     /translation="LTPLFPDEKFTLNRGKNDTLSTRIVDLFSPIGKGQRGLIVAQPK
                     TGKTVLLKEIANAIAANHPEVYMIVLLIDERPEEVTDMARSVKAEVISSTFDEPAERH
                     VKVASMVHEKAKRMVECGQDVVILLDSITRLARAYNTVSPASGKVLSGGVDANALHKP
                     KRFFGAARNIENGGSLTIIATALTETGSKMDEVIFEEFKGTGNMELQLDRKLSNKRIF
                     PAVDIMASSTRRDDLLLDQETLNRMWILRRHLADMNSMEAMEFLKDRIERTENNEEFL
                     ISMNG"
     gene            complement(26445..26864)
                     /locus_tag="PKH68_00570"
     CDS             complement(26445..26864)
                     /locus_tag="PKH68_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11458.1"
                     /translation="MKFQGINIYRLNVGNFKRGAAVTIPGIGIFVGRGMEDDLDLLRH
                     EFGHILQFRKWGFLFFWRSIAAASLKSARNQRRKFVPHMDTWTEWSANWLSYKHFGMP
                     DDWDHRRFPVAPGNESRVSKPKFMASNEKFIAEWLEA"
     gene            complement(26861..27325)
                     /locus_tag="PKH68_00575"
     CDS             complement(26861..27325)
                     /locus_tag="PKH68_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNX11459.1"
                     /translation="MEITFTTPDEYVAQLPNDKREIIQKIRIKMLENLPEGFREEIGY
                     GMIGYVVPHSLYPAGYQSNPKLPLPFINLASQKNYIALYHMSISAMPELLAWFQTEYP
                     KHCKTKLDMGRGCIRFKKPDDIPYDLIGELAGKVSVSDWIEVYERVMKRTKK"
     gene            27612..>28258
                     /locus_tag="PKH68_00580"
     CDS             27612..>28258
                     /locus_tag="PKH68_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11460.1"
                     /translation="MRNFLSLIFLFLSASYVPAQGILEYESRTFTPHWYVGANVAANW
                     YMAEGNDFLAGTDGVRFSFKDNSGTTFRATLGRRLTPVIGIRGYAGINKYKWLSSYTN
                     NPVVSFNSEFATADLTVSIVNLLEESYSLKKYDFGFFVGTGFELRNKINTPAVAVSNS
                     NLTPVGRVGLEGNFLLSSNIALTLGGELNFVNDNNNGLVAGRNYEFIPAVSVGLN"
     assembly_gap    28259..28358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28359..29072)
                     /locus_tag="PKH68_00585"
     CDS             complement(<28359..29072)
                     /locus_tag="PKH68_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNX11461.1"
                     /translation="MKLIDGKVIAEQVKAEIAEEVKGMIAAGKKRPHLAVIIVGHDGG
                     SETYVASKVKSCEQVGFKSTKITYENDVTEAELLSKIDELNKDEDVDGFIVQLPLPKH
                     ISEQKVIEAIDYRKDVDGFHPVNIGRMSIGMPCFVSATPAGIIELLKRYEIETSGKHC
                     VVIGRSNIVGKPIASLMMQKSAPGDATVTVCHSRTKNLKEFTLQADIIIAALGQPEFL
                     TADMVKDGVVIIDVGTTRVP"
     gene            complement(29179..30495)
                     /gene="ffh"
                     /locus_tag="PKH68_00590"
     CDS             complement(29179..30495)
                     /gene="ffh"
                     /locus_tag="PKH68_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNX11462.1"
                     /translation="MFESLSERLERSLKILKGEGKITEINVAETLKEVRKALLDADVN
                     FKTAKTFTESVKEKAMGQNVLTSLKPQQLMVKIVHDELTQLMGGSSVDINLKGSPAVI
                     LMSGLQGSGKTTFSGKLANLLKTKRAKNPLLVAGDVYRPAAIEQLKVLGAQLNIPVYS
                     DETSKNPVAIAEAAIRQAKLNGNDVVIVDTAGRLAVDELMMNEIAAIKKAINPQEILF
                     VVDAMTGQDAVNTAKEFNDRLNFDGVILTKLDGDTRGGAALSIRSVVDKPIKFVGTGE
                     KMDALDIFYPERMADRILGMGDIVSLVEKAQEQYNEEEARRLSKKIAKNQFDFDDFMS
                     QIQQIKKMGNIKDLAAMIPGMGKQLKDIDIDDNAFKGIEAIIHSMTPHERKNPSVMNG
                     SRKNRIAAGSGTTIVEVNRLLKQFDQMSKMMKMATMQQHKQKMKRR"
     gene            30776..>31700
                     /locus_tag="PKH68_00595"
     CDS             30776..>31700
                     /locus_tag="PKH68_00595"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNX11463.1"
                     /translation="MKKLLPLSLICLFSGLQVTAQTAIIQSVLQKIETTYAPDKRVIV
                     FDVSLQGNENEAVLKGEISEPEIHNIMITKIKEICPEIRDSIRLLPDKSFGENQWGIV
                     PLSAIYISKAPDFGAEITTQALMGTPVRILERKGGWLRVQTPDRYIGWTSTDDIERIN
                     RKQLNEYNQLQKVIVTSHHTFVLKNPNKNSEIITEAIMGNLMVVNEMKQGKKYWSVTL
                     PDGKTGYVRKEDIRPFQHGEINLSSNSIIKLGKSFMGLPYFWGGTTSRGMDCSGFTKL
                     VFFMHGIILPRDASQQYYCGTEVDTSAGWGNL"
BASE COUNT         9022 a         6755 c         6460 g         9063 t
ORIGIN      
        1 catttttttg ttcccggtct atctcatcgt tgtgctgatg atagtatccg cccagtttat
       61 agcttgcatt ccgacctttt atcaggcttt tgttgatttg aaattcagat acagctaaat
      121 atccctgatc tttgcttagc ttccagttaa tgttatacgg attattctca aaatcatcag
      181 gtgtgccgtc aaaaatggca acctgccagt tgaaagtatc tgatattttg tattgaaatg
      241 ttactcctaa tgccgttaag ggaaaaatgg gagaaggaat attgtcggca atactggaat
      301 gaattccaaa agaactgtta gtaaaaagtg caccgttttc gctgacagca aaattcgcat
      361 tcagatcctg taaacccaca gtaaaatcca acttcccgat gctttgttta taccaaagtt
      421 catacataaa ggtcagatct ccggcttcga tattggatat tccctgaaaa tcgccaacca
      481 gtgtggctga tggttcgcct ccgtgagtgt taccaacgtt tacaaacaat tgtccgcctt
      541 tccaccacct ggctttttcg gtgtcaaatc cggctttaag attggctaag cccaaataag
      601 ccgtgccggt tttgatacca cccttaaaat tgctcaccaa atcacctgta tacgaagctt
      661 cataggagaa aggtgaattc acgactactt cctcgttgtt attctgagca taaactccac
      721 ctgtaatgat attggctgca aaagcgacta tgaatgttga cttgtatatt aatttcatgc
      781 gtgaataatt ttgttgattt ctttccgaca aaagtacttc ggtaaaatca actcacaaat
      841 cactattaat aactattcgg catgaaacag gtagctatat gtaggtatat tctaattcaa
      901 aatcatttaa atcgcatttt atatgttgat tacaactaaa ttgcctttct atcaaataag
      961 cgggttttgc tttatgtcat ccagctcaat caatttattc ataatggcat aaatggtaag
     1021 tccggcagta ctgtgtattt ccagttttcg ggcaatgtta cgccggtgcg ttatcaccgt
     1081 atgggtcgaa agaaacagac ttttcgctat ttccttattg gtaagccccc tcaccacacc
     1141 cgtaattatt tccttctcgc gtacactcag ggtgttttgc tcatcggcat tttcagtcag
     1201 tgatgtattg aaaagtttgt ccagtttctc tttcagttga tcaacggtat cataaatact
     1261 taaccggtca tcgtaattgc gcaacaaatt ctcatcaata gctgaataaa ccaatgcaat
     1321 gcatttgagt ttggcattgt ctgtttctgc ttttatttta gctatatcaa aataccccca
     1381 gaaagatgga ttgacaatta agatatccgg tttatgcata ttcagatagt gattcatcga
     1441 atcaatcgta atgatttcaa cgggttgaat tctgtaaccg ggaacccgtt ttaataccgc
     1501 cgaaatgccg gttcgaatga ttaccgatgg ttcaacaatg gcaattatca attgattgct
     1561 gttcataatt tcatcttgat tttacgttca acagcttcca cttccggaat aaacagaaag
     1621 tcttccacct gattatgtgt agccaggtcc atttcacacg aaaagatatc aaacaggact
     1681 tcggtcagca aatagctgtt cccgttatcg ggatagtact ttataagtat atttttcagt
     1741 tctacaagtt tcgattctat atcgttgtga tgatttctga aaacagcgat tgtgtaattt
     1801 gagacctttt ctccattcag cagttttaga acataaggaa aagttacttt gtcttcgtat
     1861 tccatatgtt ttctgacctc cataacatat tcatcaaaaa atttcagaaa tattgaacta
     1921 tacgggatgc tctggtcctg attattcaca gcctcaatca gcttattccg aatttggggt
     1981 agtttgaatt caagaaaata agaatgagca tttttcaggt aattaatgac agatacgatg
     2041 gaaatatcac cataatcata atcaacctca atattatctt cggttaagaa attcactacg
     2101 gccagaaatg tattgcaatc aattccattg gcaatacaca cctcttttac agtctggtca
     2161 ccaaatccaa gagacaaccc aaaacgactg ataaccaata acaatgaatg attctcattt
     2221 atcaaatcac tcattttgtc agatgctgaa aatttatgtt gtttagacat atttgtacgt
     2281 tttattttat gtaaaaaaga gaaataatct tacaggaaac aatgaacttt tgttcccgca
     2341 taattataga ctaataaaaa gtttgctaat caatgaattg aaaaactcca tatcatgcat
     2401 taaccaataa ccaaataacc aacaaccaaa ttacctctac gtaggatgga gtcattctta
     2461 ccagttcatc tccctatacg catcgagcag ttgccgggca gcaacgaatg aactgatttt
     2521 atcagacagc acttcgtttt cggctacctg tagcaatgcc tgaatttttt cgttgttgta
     2581 aaagtttgat ttcaactgtt cgttgatggt ttcgtacatc cagtatttgg attgatcggt
     2641 gcgtttcctg tctaaatatc cgtttccatt tacgaaatct acatagttct gcaccatttg
     2701 ccaaacctca gctattccgg ttcgttcaat agccgaacag gttatcactt ctggcttcca
     2761 gcccgattcg ggcaggggaa acagatgcag agcattcagg tactgtcggc gagccagctg
     2821 agatttttct atattggtgc cgtcgcattt attgatagcg atgccgtcgg ccatctccat
     2881 gatgccgcgt tttattccct gcaactcatc gcccgttccg gccagctgta taagcaaaaa
     2941 gaagtcgacc atggaatgta cagccgtttc ggactgcccc acccctactg tctcaacaaa
     3001 tacaacatca aatcccgctg cttcgcacaa cacaatactc tcgcgggttt ttcgggctac
     3061 accacccagc gaaccagccg aaggtgacgg acgaatgaat gcatttttgg ccaccgaaag
     3121 ttcttccata cgggttttat ctcccagaat acttcctttg gtgcgctcac tgctggggtc
     3181 gatggccaaa acagcaagtt tggatcctgc atgtatcagg tgcattccaa gcgcttcaat
     3241 aaaagtactt tttcccgcac cgggaacacc tgtaatacct attcgtactg attttccggc
     3301 atagggcaga catttttcaa tcacttcctg agctatctcc tgatgatgcg gtagtgaact
     3361 ttctaccagc gtaacagctt gtccaagtac agaaatgtca cctttcagga tgccttccac
     3421 atactctgct ggagtgaaat gttttctcgt tctgcggggt tttgcatacg gattgatgat
     3481 gggagcatcc tgtacgcctt cgttcacaga taatcctttg tagctcggat cgttttcggg
     3541 atgttgatga tgaggattgt acattccttt atacttttta agggattgtt ttatgaataa
     3601 gaaaatcgcc tgacttcctt agcaaaaggg gaaacaggcg attttgaaaa taattctttt
     3661 atttattttt gaaagcctat tatttcttaa ccgtccaact aagcgtgtaa attaagaaac
     3721 tgttttcagg atcgttggtt tgaatggtga atgatttttt ataatcgccc tgtggcagtg
     3781 tttttgtatc aatatcaatt ttaagttcgc cggtctttcc gcctttgatg gataaacgca
     3841 tggggtgaac caacacgttg ctgttgttat taataactcg tctgatttca aggggtttct
     3901 gaccattatt tttgataatt gcctggcctc ttaccttgct gccttgtttt attgaaccaa
     3961 aaagcaagtt gggagattta atttccaaaa ttggtgactg ttgtttttct tcaatggtca
     4021 ttttactgaa gtcgtccaca acattggcat tcacagtgat tttatgaagt tcgtccgcaa
     4081 ccttctttcc atccagcacc aggtatatat tgtcgaaaac gggtccccat tctgcacatt
     4141 tttttgagtc gaaagtaaat tcaattctgc cttcttcgtt gggattgagc acggccggtt
     4201 tagcttctgc tttcaggtaa acaggcaaat cagccagtga aacggtcatt ttggattttg
     4261 atccgttttg aataagcaag gtgtttgttt tggtacttcc tttagaaacg ttattgagca
     4321 gtacggtttt tgttttcacc ttcaaatcac ctatctgaat ggggtaattg ttcacagcag
     4381 gcgacgaagc agagttcacg gttcctttga tgagcaaact ttccacttcg ttggaggcat
     4441 tgctgtaaac cgttatcgtt tttgtaaatg ctccgggtct gccggccgga ttgtaggtta
     4501 ctgtaatttc gccttttttt ccgggctcaa caggggtttg ggtccattcg ggggtagtac
     4561 atccgcacga tgcgttcaca cgttgtacca acaaagtggc attgccgctg ttggtgaatt
     4621 caaagacatg cgttgcttta ccgtctgtct cattaattgt tccaaaatca aaggtttttt
     4681 catcaaaggt aatcacaggt ttctgtgccc aggtggtgag tgtgacaagt actgatacaa
     4741 tcagcaaaac tccaaatttt ttattcataa aattcaatat tttaagtttt caataattat
     4801 tcgataaacg gtgcaaatgt ataaaaaaat gtcaatttat tgtccataaa actctgtaaa
     4861 ctttgtcata tctttttaat aaaaatatat ataaaacaga aatggagtat atttgcaata
     4921 tgaaatttct aacaaaattc ttacctaatt gttttccaac agactatgaa cgaagatttt
     4981 aaattgttgt ccgattctat attcaactat acatcaacat gttaccggca tttgcacgct
     5041 caccccgaac tttcttttca ggaatatgaa acctccacat tcattcagaa gcaattgacc
     5101 gatatgcaga tacctttcag gtcaaatatt gccggaacgg gcattctcgg tagaattgaa
     5161 gggagaaatc cgcagaaaaa aatcattgca ttgcgggctg atatggatgc gctgccggta
     5221 caagaagcgg taaacattcc ctggaaatca aaaaatgata acgtaatgca tgcctgtggt
     5281 cacgatgcgc atgtagcttg cctgctagga gctgccaaaa ttctgaagca ggtttgtgat
     5341 gaatttgaag gaaccatact gctgatattt cagccgggcg aagaaaaagc acccggtggg
     5401 gcttcgctta tgctgcaaaa cgggttgttc gatgagtaca aacctgaagc aattattgcg
     5461 cagcatgttt ccatcgatta cccgaccggc acaatggcat ttctctccgg caccatcatg
     5521 gcatcagctg atgaaattca cctgaccatt cgcgggaaag ggggacacgg tgcattgcca
     5581 cacctgtgta acgataccgt gttggcggca tcgcagacgg tcgttgcatt acaacaggtc
     5641 agaagccgcc tttgccatcc gctcacgccc atggtgctta cctttgggag acttattgcc
     5701 gaaggagcaa ccaatatcat tccttctgaa gtacaccttt cgggaacttt ccgtacagtg
     5761 gatgaacgtt ggcgaaaaga agcgctggaa catatccgca gaataattac cgaaacctgt
     5821 tcagcccata gttgcactgt ggagatagat attcccgatg gttatccgtg tgtggtcaat
     5881 gatgcgacat tgactgaaaa aatcaaaggt tttgcaggtg aatatgcagg tgaagaaaat
     5941 atcgataaac ttgaattacg gctgacatcc gaagatttcg gatttttcac acagcaatat
     6001 ccgtgcagtt tttaccgttt cggggtgaaa ggtgatacca atcaggacat gggtggattg
     6061 cacagcccaa cattttgcat cgacgaaaag gctttgttga cgggtgcagg agggctggcc
     6121 tatattgccc gtagaatgat ggattaattc ttattccatc acgtggtaca ctctgcggta
     6181 aatggtggat ttgatttttt cataattagc cacaacaaac tccacctgat cggtcatcag
     6241 atacaagggc tgataatagc cttgcttgtt gtatggcggt tgcaggtaat ctaccggaac
     6301 tatttcgtac cccagccggc gataaaattc aatccttcga gcggccatct catcatcggg
     6361 cagttctact tcgaaaacca ggggaatatt ctgtgttggt ttcatcatat tcattacctt
     6421 ttcacccagt ttgttgccgc gtatggcttc ttcgatggca aaatgctcgg catacaaaaa
     6481 ccatccgaaa ttccagtaat tgaaaaatcc cacaaaggaa ttattcatca atactgcatt
     6541 gcagaaaaaa tcgggctccc ggcccaacaa ggctataaaa tcatcgtcag tatgtcgttg
     6601 attctccgga aacgcttccc gataaagtgg caacagtttt tggatatatt cgtcattcgc
     6661 cgatttgatt tgaattattt ccatggtgat tggttaattt agcacgcccc agctccgaaa
     6721 tgggaaatta gagaagagtg atttttatgt tattggttat tatgttatta agttaccact
     6781 tacctgttcg ttaagttaca ggtcatgaac ctttggaatc agctcattga ttatcaagtc
     6841 aatggttttg gatcctggtt tcaaaattat atcaaaattg gagatttcaa tactttttat
     6901 tgtaatattt tgactggaaa gaacattttc tcatcttcta agaaaaaaac aaacggcttc
     6961 acctgttgaa agtgaagccg ttaatcgtat taatttttat tggaattaat tgcaaccgca
     7021 accacagcct ttgctgtcat catcgccgca accacatccg tcgtcaccgc aaccgttgtc
     7081 atcgcaaccg caaccacaac cacaaccgcc accgccaagc agcgatttca gttcttcgtc
     7141 agttgcatcg cgtacttcca gtacttttcc cttgaagtga agagtttctc ctgccagcgg
     7201 gtggttcagg tccacagtta catgggtatc gctaaccgaa actacctgtg cctgaagcct
     7261 gtttccttca ttatccatca atggaacaac tccgccttcg aataccattt cttcgtcgag
     7321 ttttccgtca atttcgaata ccgaacgttc cagttctacg actgcatcat catcatattc
     7381 gccgtaagca tcttcattgc tgatggtgaa atcaaaggca tcaccttgct caagcccggc
     7441 aatatttgat tcgaatttag gcagcatcat tcctacacca tagataaaat tcaatggttg
     7501 ttcgggagtg gctctttcca tcagttcgag ttctccgttt tcgccatcta cgtacagttc
     7561 gtattcggca tttactactt tattaatttc aattttcatt tttttttgtt tttattgatt
     7621 tgtatatttt tttcgatttt tccaatttca atagttgata taactcaata ccagctaata
     7681 ttgttcgacg aacttcgatt tccctgtttt tcatacttca aatcctgaag catgcttgag
     7741 tttaccccaa ttctgaaatt ataggtttta tagggtccga atggcaccac gttacccgtc
     7801 aatgtccagc agtgaaggtt tcgcgtgata ttgaagctgg tatatgacat ttgtttagcg
     7861 ttgaaatcat acgaagtggt tccgctgaat tgccagtttt gagttaattt aatattaccg
     7921 gttatcccca cattgtgctt gatttcctgt ttgtattcca gtttttcata attgaattcc
     7981 gatgaattac caaagccaat aaaataatta aaactcaggc tccacggtat ttctaccttt
     8041 tcgtaccctt cggcatcggt tttcacctct gcgccgctct cattggattt attatccttg
     8101 ttctgcatat tgttattatc gcggttcatg ccttcattga gctggctgtc atccgcctgg
     8161 tcgtctccct tcggtttact ctttttatct tttcgcttga atgtctcatt ggacaaggta
     8221 taactgtagg aggtggaggt tcccaaaaag cgggggaact tcccgtggtt ccaacggagt
     8281 tgattcactc ttcgaatggc tttaccatcg ggcgaagcat acatgtaggg atcaaaagct
     8341 ccacttaagc tcaatgtata tgccttaccg aattttatac gcagattagc actgatattg
     8401 gacaggcgca tggagtcggc cgcaaaattg tatgcactgt tcagcgaaaa attgtcaatc
     8461 aggcttatta tttttgtcgg ctgtaaccct gtcgtatcct tatcattttt cactttcatt
     8521 tccaggttat tatcaagcga gaatgagagt gagttggact ttccgcggcc cggtgctcca
     8581 ctcgggaaac gggagtaaaa aatctcttcg gaagtaattt tggtggggtc gccatccttg
     8641 atcaccgatt taatataagt atcccagtat ccgtatttca aatcaccgaa atcgggtgtg
     8701 agactaaaac tcgcgttcgg tttggccatg tggcggattt tttcaatttt atcgccaaaa
     8761 attgccttta aaggggtgta cataccataa agagtagtat tggccgacat gctgaggttg
     8821 taatcccaca cgcggttgaa accggtaact gtggtggtta cttccttttg attgaccaca
     8881 tcccagtttt ttcgctgcga agtaaggaac catttttcgc gatagttgaa cgaaggggtg
     8941 aatgtgatgt atttcaacac attgaatgat gcagaaaccg gaatactgtg ttgtgccgaa
     9001 ttgttccagt cacgggcaaa tgaagacttc agcagcttat tttctttggt ttgaatgctg
     9061 ttgacaatgg ttccggaata ggacatggat attttttcgt accatcgttc tttccctaca
     9121 gcattttttc ttttcagcgg gaaaagtcgg ctcatactga tagccacatt gggcaacatc
     9181 aggttgatgg tagagtcctt ggttcgttga gtaaccgaca tattggctgt aaaactgaac
     9241 ggattgttgg gaaaccggcg tgagaatgac acactggaac ttttggtatt atcagcattg
     9301 gccggggtat aataactgtt aatattactc cggttatatc cgctggtcgt aaagttgaca
     9361 ctggctgaga aagtactgaa cggattgacc ttggcattct gcgaatggga ccattgaatg
     9421 cttaaattcg gatttttctg ataatcgggc atatcctttt cgccgattac atcctcacgg
     9481 taactgatac tgacacctcc gttgaattta tatctcttca gataattgga cgacaaattg
     9541 agtccccacg tcccgcgggt atatacatca cccttgatgc tcagatcggc atagtcgtta
     9601 atggcaaaat agtaacctcc gttgaccagc ccgtaaccac gggtgatgtc cgtatcaaaa
     9661 ctcggcattt ccactccgga cgaataattc ttgttgaacg ggaaaaaacc aaacggcacc
     9721 gccaccggca aaggaatatc cagtaacacc atgtgtgcag gaccggttat aatatagctc
     9781 ccgggtttca ccttggcttt tgacaggctc aggtagaagt gtgggtgttc gtgattatcg
     9841 caggtagtgt acattccctg ttgcatacac agcacatcgt cctcggtttt tttagtttta
     9901 tcactgatta tgtatccctc gccctgctgc gtcactgcct gacggataaa ccctttccgg
     9961 gttctgagat tataggtgag ttcattagca ttgtattcct gatcgccatc gttgatgatg
    10021 ggttttccct gcatcactcc cagactatcg ggtacaccgc gggcaaaaat ggtggtgcta
    10081 tccatgctta tacgcacata atcagcctct ttgaggttta tctttttgta actcagattt
    10141 gtttttccgt acaaaaaacc ggtgccatca cccataaaca caatagagtc ggtggctgtg
    10201 taagcaattt cggcatcaat ctgattgggg cttagttttt tggtaatttg cgcggtatcc
    10261 ttactggctg ccattttcag actatcgggt agctgaacag cattctgggg ctggctcata
    10321 gcggtggctt tctgtccata cgcattctgt aaatacgtaa aacaagccat taaaagaaat
    10381 aaaactgtaa gttttgggtt atataattgt ttgcagcttc gcattcagta gtaattcgtc
    10441 gaaaaaacaa gctgcaaatt taatcaaaaa ataggtgtac ttaatcaatc agctctaatt
    10501 ttatgaataa tttgatattt tacaatttat taaatgcctc taaacaggat aaaattacta
    10561 cttttgcatt cagttttcta cgtatatttc actcatcatt cttttactca aaaacaatga
    10621 aatacttccg ctacattaca ttggttatac tattattttt aacttgtact gtatcttctc
    10681 tgtacgctca aaaaaaattc actgttgtga ttgatgccgg tcacggggga catgactcgg
    10741 gagcagtagg ccgttattcc aaagaaaaaa atattaacct ggcagtgtca ctggcacttg
    10801 gcagcaaaat agaagccaac taccccgatg taaaagtggt ttatacacgc gataaggatt
    10861 ttttcctcac cttgcaggag cgtgctgaca tcgtgaacca aaacaatgcc gacatttttt
    10921 tctgtattca caccaatgcc aacaattcat ccaccgcttt cggagccgaa acctacacat
    10981 tgggtttgca caaatcacaa tcgaatctgg acgtagccat gcgtgaaaat tctgtaatca
    11041 ccctggaaga tgactataaa accaagtatc agggatttga tccgggtgca cccgacacct
    11101 acatcatgtt tgaattcatg caggacaaat acattgataa aagcattgag ttggcatcgg
    11161 ccatccaaaa tcaatttgtc agggccagca ggtatgaccg cggagtacgt caggccggat
    11221 tttgggtatt gcataaatcg gcctgtccga gtgtgctcgt agagcttggc tttatctcca
    11281 accgcgctga ggaagattat ctgaacacca acgaaggccg ggaagaaatg gctaattcaa
    11341 tcttcaaagc attcgaaact tacaagcgcg atcacgacaa aaaatcgggc ttcaaggtgg
    11401 tgccgctcaa aaaagaagat acacccgctc cgaaaaaaga ggatgttaaa cctgttaaag
    11461 tcgataatcc ggtgatagct gcaaatgttc caactaccga tactaccaaa aaaacattaa
    11521 agaatgaggt agttaaagtg aataaaccgg atgtacctgt ggccgatgtg aaacagaaca
    11581 atcaggtaat attcaaaatc cagctttttg cggtagagaa aaaactcaga aaaggctcat
    11641 cagaattcaa aggactgaat gacaccgatt tttatgttga aaaaaatctt tataaatata
    11701 cctacggtga agagacctcc ttcagcgaaa taaacaaact ccggaaaagt atctcgaaaa
    11761 aatttcccaa tgcctatgta attgccttca gaaacgggga aaaaatagcc ataaaagatg
    11821 caatgaaaaa ataagaatat tcatttgaca attagaaaca acattcaatg aaaaaaaata
    11881 tttttacccg cgaagtaaaa gtgggactga tggtaattgt tgccattttc atactttatt
    11941 tcgggttgaa ttttcttaaa ggaattgata tttttcagaa aactgccatt tattatggtc
    12001 agtacgaaga tataggcggg ctggttccct cggctccggt gtacatcaaa ggatataaag
    12061 ccggacaagt ggaagaggtg aaatatgatt tttcgaagaa agaatcattt atcgttaaaa
    12121 tatccgttaa cagtgatatt aagttgccag tgggtaccac catgcaaata gctgatgaag
    12181 gattgatggg cgggaaggtc atacagctgg tttacgcacc tgtaactgcc agtcaagccc
    12241 tgtacaaaca aaacgatatg ctgcctacca ccgtgagcac cggccttatg gcaagtgttg
    12301 caggggatct gatgccgaaa attgacaaag tagctacaca ggcagattca ctgttgtatt
    12361 cggtgcgttt gctcatcgaa cgcaaagaaa taagcaacag ccttggttcg atagaaaaaa
    12421 ccactgccga ccttgccgct tcctctgccc aactgaaagg aatgctaaat aatcagctgc
    12481 cttccatcct caatgatgta aatgtagtca ccggagactt caaacaggta agcggaaaac
    12541 tgaaaaatat tgattttgca cagacatttg ccagcgtaga ttacaccttg aaaaacctgc
    12601 agaacatgac caataagatg agcagtaacg aaggcagtct gggactgctt atgaatgata
    12661 aaacactcta tatcaatcta tcaaatacag ctggtgctgc cgataaacta ctgatcgatg
    12721 taaaagaaaa tccaaaacga tatgttaact tttcggtgtt cggcaataaa aaataaatag
    12781 aaattattga tccggattga cacaccttat ttagaaaaca tataaataga caataaattg
    12841 taaatatttt tttcaacaac aaattcgctt atttacaact tctcttacag atttacttaa
    12901 attcattgaa atttaacatt aaattattaa catcttaata atcaattcaa ttcgtttttt
    12961 cacaaaccgc aacatcttga ctttcacaca tttactctat acaactgaac accaaacagt
    13021 taaaacaaac aaaaaaatac gtaaaaaaaa agatttgttt attcgaaaaa agttttggaa
    13081 ttttgtagaa ctgaaatgcg gttattgatt tcagaaaatt tcagaaaaaa aacacaccca
    13141 atgtgactcg acctcggtcg agttttttat ccttaattag taaattaatt tgtaaaatat
    13201 caacatgagt tatcactacg agttgtggaa tagttgcctg aacattatta aggacaatgt
    13261 aaatgaagcg tccttcaaca catggtttgc acctattgta ccggtaaaat ttgaaaacaa
    13321 aatctttgta cttcaggttc caagtcaatt tttcgtggaa tatattgaag aaaaatacat
    13381 tgatctgcta cgaatgacac tgacacgcgt agccggtaac ggaacccgtc tggaataccg
    13441 ggtgctgata gacaaaacga gtggaacagg tactcagata cccagcacag ccgaacattc
    13501 gagaccaaac accactaatt acaatgcaca acagaataca attgtgtcgc catacaagcg
    13561 aatgcagttg cccgaaattg atccgcaact caatccctca tacaatttca acaacctgat
    13621 agaagggaga agcaacaagc tggcacgtac agcaggtatc agcattgcaa acgatcctgg
    13681 taaaacagtg tttaatccct tgtttatgta cggaaaatcg ggtgtgggta aaactcacct
    13741 tgccaatgcc atcggggtga tgactaaaca acttcaaccc cacaaacggg tgctgtacgt
    13801 ttcggccaat acatttcaga tacaatatac cgatgccgtt cgaaataaca cccagaatga
    13861 ctttctgaat ttttaccaga ccatagatgt gctcatcatg gacgacattc aggaatttgc
    13921 cggcaagaca gctacgcaaa atacattttt ccacattttc aatcacctcc atcagcttgg
    13981 taaacagctg gtacttaccg ccgaccgctc accgctggaa atggctggtc tggaacaacg
    14041 actgcttacg cgctttaaat ggggcttatc cgccgaaatt gaaaaaccgg atttcgaact
    14101 ccggcaatcc attctgaaaa gtaaaattta tcgcgacggt ctcgaaattc cggaagtagt
    14161 ggttgattat attgccgaaa atgtggtaga caatgtacgc gacctcgaag gagtgttgat
    14221 ctcgctgctg gcacattcca ccctcaccaa tatgcctatc gatatacggt tggccgaaaa
    14281 gattatcgga cgcattgtaa acatgacgcc caaagtaacg acaattgaac aaatccgcga
    14341 agtagtctgc gattatttca aactgtcggt agatgccatc tccaccaaat cgcgcaaact
    14401 ggaagtggtt caggcacgtc aaatagccat gtatctgtcc aaaaacatga caaaaaattc
    14461 actgtcgatg attggtaact gtatcgggca gcgagaccac gccaccgtgc tccatgcctg
    14521 caaaaaagtg gttgacctga tggatacgga taaacacttc cgcaaaaatg tggaagaaat
    14581 tgaggacaga cttagaaaat aactgatata ctgacaaata aactatcaga agaccggcga
    14641 catacaacgc cggtcttttt ttatgaattt cttactgaat tcattccgtc gaataaaaaa
    14701 aatgttgtat ttttacaccc gtaaactcaa caaattacag ttatgatatt taaattcaca
    14761 atcatttcag acgagaagga cgacttcctg agaatcatag aaatagattc cgaagccact
    14821 ttcctggatc ttcacaatgc cattctggaa tcggtagatt atgaaaaaaa ccagatgaca
    14881 tccttcttcc tgtgcaacga cgactgggaa aagggtcagg aaatcacgct tgtggaaatg
    14941 gaatcgagct cggaatacga taacctggtg atggaagaca ccgtactcga cgaattgctg
    15001 atggatgaaa agcaaaaatt gatttttgtt tttgatatga tctcagatcg tgcattcttc
    15061 atggaactaa ccgaaatgat acccggtaaa gatattgatg aagcggtgtg taccacttca
    15121 accggctctg cacccgtgca gatagccgaa gaaacggatt ttgcaactcc tcaaaaaatg
    15181 cagatacctt cggatgaaaa cttctatggt gatgaggaat ttgaaatcga cgagcttgac
    15241 gaggaaggat tcggcgattt gaattttgat gaaaacagtc tgtttactga cgatattcgt
    15301 taacaataac tcaaacaaac acattaaaca gaaaatattt cagctatgaa gtgttttctg
    15361 ttttcttttt actaatgcac accttactag tcattacagg acccaccggc gtgggaaaaa
    15421 cggcagtcag cctgctattg gccgaaaaat tcggttgtcc catcgtgtcg gccgattccc
    15481 gtcagtttta tcgggaactg aaaattggca cggccgcacc aaccgacgat gaactggagc
    15541 aggtgaagca ttattttatc ggtacccatt ctatatttga cgaatacaac gccggacagt
    15601 acgaggaaga ggccatcagc ctgctggacg aattatttga gaaacacgag gtggtgctgc
    15661 ttacaggagg ttcgatgatg tatgtggatg ccgtatgcaa cggactggac aacatcccta
    15721 cagttactgc tgacgtacgc caattctggc aaaaacaatt tgcatcccac ggattggaat
    15781 ttatacagca cgaactgaaa cggctggacg aaaagcacta tgccgaagtg gacctgaaaa
    15841 atccgaaacg aatcattcat gctctggaaa tatgcagtat gaccggaaaa ccctactccg
    15901 acctgcgtac cggaaaacga aaaatcagaa atttcaatat tctgaaaata ggattgaaca
    15961 ggccaagacc cgaactgtac gaaagaatta acctccgggt agataaaatg atgcatgacg
    16021 ggttgctgga ggaagctaca cgctattatc cctaccgaca cctgaacaca ctgaatacgg
    16081 taggttacaa ggagattttt gaatatctga atggattgtg cgacctggat acagccatta
    16141 acctgataaa acaagacagc cggcgttatg ccaaaaggca gttgacgtgg ttcaaccgcg
    16201 acaaggaaat caactggttt caccccgaag aggtagaaaa nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn tttgtggcct attgtgtttc caacaattac tacggagtgg
    16381 aaaatctgtc gctcataccg ggcgaagtgg gggcgtcggc agtgcagaat atcggggcgt
    16441 acggcatgga agtgaaagat gtgattgaga ctgtggatac cattcatacc gaaacactta
    16501 atgaacggat attcaccaat gaagaatgtc gttacgggta tcggaaaagc attttcaagg
    16561 aagaactacg ggggaaatat attattacca acgtaacttt cagactgaaa aagcaaccgg
    16621 actatcaact tgattatcag cacctcagag aggctgtatt gcaacgcgga gaaataaatc
    16681 tggccaacat acgctctacc gtaatttcca tccgggagag caaactgccc gatccgtcaa
    16741 tcacgggtaa tgctggcagt ttctttatga accccgttat accgaaagaa aaattcgaag
    16801 aattgcaagt atcataccct accatgcccc actacccggc ttcggaaacg gaggtgaaag
    16861 ttccggccgg ctggttgata gaacaatgcg gctggaaagg cagatcggtt ggtaacgtag
    16921 gtgttcacag ccggcaggcg ttggtaatca ttaatctggg tggtgcaaca ggaccggaag
    16981 ttgccggctt agctgcccaa atcagagaaa atgtatttca acggttcggt attgacatca
    17041 ctccggaagt aaattatatc tgattatttg cccactccct gaacggatgt tgactgaggt
    17101 atgaattata gtattttcgg tcgccggtaa cttcccgccc cagccaggca ggctttgtga
    17161 attgttcctc ttgagattgc agctccagtt cagccattac cagtccttca ttttcgcctt
    17221 cgaacacatc cacttcaaat actttgccct caaaaggaac ttcatagcgt attttgtcaa
    17281 ttacgcccga accgcacaaa gcgaggagct gagtggcatc atcgaaggtg atttctttct
    17341 cccattcaaa acggctaaac ccgttgtcgc ccgattttcc cttgatggta ataaatcccc
    17401 ggtctccttt gacacgaatt cgtacggtgc gatcgggagc cgagcataaa taaccctgag
    17461 taatgcgaaa accagctgaa acaaagggct tgaaatcacc ggcaactaaa aactttcgct
    17521 caatttcgat attatgtgtt gactgcatat tatttcgtat ttttcagttg attatcttct
    17581 gcaaatcact ttgtaaggac agtattcgca agttttggca ttggtacact gtgtaaaagg
    17641 aacttcgggg ttgaaaattt cttcgagaca tacggtcaga ttgctgataa aaccggactt
    17701 ataatcctca aaattatcaa cggcacctac cccattggta cccgatttgt caatcagtcg
    17761 tgtagtgaaa ttggttttaa atagatcacg aatatacaga atttcgggta caagttgctc
    17821 tgtatcattt gtatgcccgt aaagcaggca atacagaaat gtctgcagca caaattttgg
    17881 tcgtttatcc atatcgcgtt taaatacatc gtcgggcgac ttaaattcaa gtgagccgga
    17941 acctgttttg taatcgagta tgcgtgtcac tccctgccgt tcatccacac gatcgatata
    18001 tcctttcagg ttcacggcgc tttcaccacc gtgtatcggg aaccgcatcc gttcttttcg
    18061 ttcggactga atataattga aaggtgaatt ttctgcatct tttttcagaa tttgaattac
    18121 atattttcgg attacacgtg ccaccagcag gtagttacct tccaaaacaa ggtcaggatt
    18181 ttttcctttc ataaaataat tctccgaaaa tgcagtgcgt atagcccgat caacggtcag
    18241 cggatttgtc cacaattcct ccaacgcagt tttatggacc gtttttcctt cgaaggattt
    18301 atacaaacct tccatcaccg catgaagcag tgtgccgaac ataccggctt caatggtttc
    18361 ctgaacttct tcgggctcgc gcatctgctc cacctgtgtg aggtaaaact gcaacggaca
    18421 atcgatgtag ctgttcagtg aagtagccga cagtgcgggt gcatcgttgg cgtcactcag
    18481 gtaaggctga agttttgcca ttacttcagg cgttttttca acctgcattg tcctgtcgga
    18541 aggtgttcga atatcgtagg caacattgat cagccggaat tgaactccgt aatgataccg
    18601 aagctgatga atgtaacggc tcacttcacc ggtctgagca ccttcgttgc gactgtcgta
    18661 cagaaaaatc aaccgacggg cacccgatat gagtcggtaa aaattatagg ccgaaatggc
    18721 atcgtgatat tcagctgtgg gcaggttgaa tgcttttcgc aggttgtagg gaataaaact
    18781 gttggaagtg ctttttttag gaaaaacgcc ttcattgaac gagcagataa tcagattttc
    18841 gaaatcgaga ccacgcgttt cgagcattcc cattatctga agtccgttga gcggttcacc
    18901 ttcgaatgga atggaaatgc cgccgataag ctggcgaaca agcttcagga gcgtatcaat
    18961 agttatttca gccgtgtcag ccacagatgt tatcacttcg ttcaggcggt tgagtgagat
    19021 ataatactga aacagaaaat cgcattccag tctgaaatca ttttcggcat cggctttatg
    19081 ctgccaggct atatttaact ttttgagaat gtcaagtaaa tattccacaa aaccgaccga
    19141 tgatgtatga ggtgtaaaaa tggcagccag caactcattt ttgttgaaag cttcgggctc
    19201 cacatacatg gcattggttc ggatcatcat ttcggcgatg ctcacggcat cgtcagagca
    19261 caacaaatgg atatactggt ggttcaaaat accggaaacc gtatgatggt aaaattgctg
    19321 acgcttattc atgcggcgct gcagttcaaa tatattctcc aacaggctcg aaacgggcgt
    19381 ggcattgagc gggaaaccca tggtaacatt aatcttttcg atagaaggag gtagtgaatg
    19441 gagcagcggc accaacatac tctcgtcggg caatactacg gcggttttta tccaattctt
    19501 atcctcagag cgatcgggat aaagttcgtt cagcagtcgg taaacttcct tggtttgccc
    19561 tacagcagag ggcatggaga taagctccca ggatttattt ttcagatccg gcgtttcatt
    19621 atccagttgg agtgccgaac gaaagttttg tatgttttct ttataaaaaa gcgatgcctg
    19681 attgtcgctg tcacgcagtt cggctgcttc gtagtcccag tagaaatcgg cctgcccgcg
    19741 tttctgaagt tcggtaaaga gtaatttttc gcagggattt aaggcattga acccgacaaa
    19801 aacaaatttt ttaccggaaa aatagtcagg atcttcttta cgtttcagct gttcgctcac
    19861 ttcgcggaat atcatgcctt cggtggctgt gttttcagca aaaagttgac gccgaaattc
    19921 ctcgtaaagg ggtaataata ttcgccaggt ggctataaat tcctctttcg ttttcccttc
    19981 gataatggga ataaaatttt cccagaaatg ctttatcgcc gatttttgtt cttcggtgaa
    20041 tacatcgaaa aacaaatcaa gatctttcaa atctttcaca ttggtaaaca actgacgggc
    20101 atcaatccga tatttgtcca cctcattgaa atcggccagg agaatttcac cccagtataa
    20161 gaacgtatcg aacggttcgt cgctgccact cacctgccgg tagatgctgt acagccgaaa
    20221 aagctcgcgg gtgcggtcgg caacgcggcg ctgtgaagca ttgaaaaaac agtcgtttac
    20281 ggtaagaatt gcgggcgaaa aaaccggttt atcaattaat tccgataaat atttctgaaa
    20341 aaaaagtccg gctctacggt taggaaaaac aaaggtataa ttcaatatat cagtatcata
    20401 ctgatggtaa tatgctttag ctacgcgata caaaaaagag ttcataaata taattttaag
    20461 aaaagcctta aggaatggct aaattacata attttattca atccggcaga aaaaccggtt
    20521 tttttgttac ttttgttcac ctaaaaaaaa gaataacact attttttatg attggaacca
    20581 ttgtcaatac agctaccgtc attgtgggcg ggtgcatcgg attgctcctc aaaaccggca
    20641 tgccagaccg cttgaaaacc atttattttc aggctgtcgg attattcaca cttgctatcg
    20701 gcatcggcat ggtttggaat atggagcata ttctcgtagt cgtttcaagt ctggtgatcg
    20761 gctcaatttt aggcgaatgg tggaatatag aaaaaggaac cgatgagctg agcgagtgga
    20821 ccaaaaagaa acttcatata ggcagcgaaa aattctcgga agggctgacc acttcttttc
    20881 tcctgttttg tattggtgca atgactatca tgggagccat agaagaaggt acggggggca
    20941 cttcccgatt gctgatgacc aagtcggtga tggacggttt ttcagccatc atactggctt
    21001 ctgcctttgg cgtcggggtc gttttttcag ctattccact gcttctgttt cagggtggac
    21061 ttacgctggc agccggattt gccggtgatt ttttgtcgcc cgacatgatc agggcgctta
    21121 ccagcattgg aggcattttg ctcatcggac ttggaattaa catcctggaa atcaaaaaac
    21181 tgcgcatcat gaatatgtta cctgctctgg tagttgtggt gctgctgatg tggtttgttg
    21241 ccaaattcaa tattcaggta ctatgaatat aacacaggct ttcatctggt ttctgatcgg
    21301 tttcctgctc gtcaacgggt taaaatttct acataccatc ctgggcagcc ggatgaaaca
    21361 taagcgaata aaaaataacg aaatcccggt gcggggatat tacaagcagg cctattttgg
    21421 aatgctcagg ttccagccct tttactatgt gataatctgg ctggtgtgca gctactttta
    21481 tttttcgggt cacatttcag gcaattcctt tgcagatgca atgatcaccg gtatttcctg
    21541 gtgggctttg accgtagttt gcgagatgct gatatgggta ttggtcaatc atacgatgaa
    21601 tttaagctgg aaagagatgt atctgcaatc gcaaccgtgg atttcgctca cctattatgc
    21661 cgtgttggtc agtccgctga tcatttcgct ggtgcttcac taatttcacg ttagtcaaac
    21721 actggcagcg cctcacccac cacaaagtta cttccgccga caaacacaaa atcattttct
    21781 ccggcttctt tcagagcgga cttaacggct tgttctactg ctgaataagc atttccattc
    21841 aaacccagtc tttctgcttt ttccttcagt tttagtgccg gtaaggctct ttcggttgta
    21901 gcatttgtaa aataatagac tgcatcgcgg ggtagcaacg acagtacgcc tgagatatcc
    21961 ttgtccttga ccataccgat cactatccgc aatttttcat agttctgact tctcaactgg
    22021 tgcacaatat actcaattcc gcccttatta tgtcccgtat ccagcaccac cgtgggttta
    22081 tgcctcacaa gctgccatct tccctgcaga ttagtaagcg gtattacatt ttccaatcca
    22141 tttttcacat cttccctgta aatggcaaaa ccgagttttt gcagttcttc aacggccgtt
    22201 aatacggttg caatattttt cagctgatat tctccacaca aaccgactat cagtttttca
    22261 ttatccagcg tttccacaat cattttatca gctgaatatt caatgaattt taccggtata
    22321 ttttcttctg caaaaacaag aggcgaatgg tgcttttgag ctttctcaat aaagaccggg
    22381 cgtgtttcgg gcacaacctc cccgattacg accggtgttt cgggttttat aattcccgct
    22441 ttttcagcgg caatctgtgc aagtgtattg cccagaaact ccgtgtgatc gaaactgata
    22501 ttggtgatca ccgaaagtgc aggactgacg atattggtac tgtcgagccg tccgcccagt
    22561 cccacttcta tgacagcaac atccaccgcg cagtcggcaa aataccggaa tgccatggcc
    22621 atggtggctt caaagaagga tggctgaatt ttatccagca tcttttcatt tttctccaca
    22681 aaatcagcca catattccgg ttggattttt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn naagtattct atggcttccg ggtagttcat ttcttgtttt tattataaat
    22861 tatcaatgat tttacttaat gggattatcg gcggcattga catctgattg ctctgtgtga
    22921 taaatgatac cggtcgttgt tttatttcaa atttctgtac ttttagcatt attttaatta
    22981 aaaaaatgca caaaatacca tccgctgccg ttataaattt tatctttgta atccaactac
    23041 aaaaatacat cattttcaat ggatactctc cacaaagttc agctatttat cgaaaacaat
    23101 aagctgctca cccctcagag ccgtttgata gtgggcgtaa gcggtggcat agactcggtg
    23161 gtgatgctta atatcctctc ccgactggga tatgagtgtg tagtggctca ttgcaatttt
    23221 catctgcgtg gcaaagaatc ggaccgcgat gaagaatttg ttgcaaaatt ggccgaaaaa
    23281 atgggattgc tctataaaaa aacggatttc gacaccgtca gttacgccaa acgtcgtaaa
    23341 atttccatcg aaatggctgc ccgcgacctg cggtacgact ggtttgcagg actgctcacc
    23401 gatctgaatg ctcaggctat tgcagtggct catcatgccg acgattcgat tgaaaccatg
    23461 ctgatcaatt cggtgagagg gtctggtttg cgtggactgt atggtattga tgcccgcaac
    23521 ggaaatgttg tgcgtcccct gctcggttgt acgcgtgaag aaatagagat ttacgcccga
    23581 aaaaacaaac tcagccatgt atttgactct acgaatgcaa gcagtgacta ccttcgcaat
    23641 aggttcagga atgacatcct gccggcactg gctcacatca atccttctgt aaaacatact
    23701 tttctggaaa acatgaaccg gatgcgcgga gcctggaaaa tctacaatca aaaaattcag
    23761 gaaataaaag cagaaatttg ctctgctcat ggcaatcaga tgtatatcaa cataccaaaa
    23821 ctattggaac aggctgatgt taccaccgtt ttattcgaac ttcttcaacc ttatcatttc
    23881 aatggcaagg tagtggaaga tattgtcaat agtttaaaaa acacgtcggg tgcaaggttc
    23941 ttttccgata cacactgcgt actgaaagac cggaatcagt tgattatcga agaaaaagaa
    24001 gtggacagcg atgtggaata ccttattgaa aagcagacca aattcatcaa tgaacctatt
    24061 gggatggaat tcagacagtt tgcggccggg aaagattttc aggtttcccg ccaacccgat
    24121 aaaattcata tcgatgctga caaagtaagt tttccactta cactccgacc ctggcgaaaa
    24181 ggcgatgttt tttacccttt tggcatgacc gggaagaaaa aagtaagcga tttttttatc
    24241 gatgaaaaaa tcaaccgctt cagcaaggaa gacgcctggt tgcttttatc gggtaaggaa
    24301 atcgtgtgga ttgcagggct caggcttgac aaccggtttc ggattacccc cgaaaccaaa
    24361 cagatacttg aagtaagtct gaagagcaac aattaatttt attgttcaat atttgtggta
    24421 ttaaaataaa tattgtactt ttgtcgcgtt atcattttac aaagtcttac gtcgtccgac
    24481 taagataaaa catcaaaaga atttttcaac taaaaacata tctcgttttt tcccacagta
    24541 tcagggaaat ctccccaata cgtattattt aatcatttta tgacgaatta caatattttg
    24601 caattgaatg cgaaagaact ttctgaactg aaagaaatcg ccgctgagtt aggactgaaa
    24661 gtatccaatt cggccaccaa agaaaaactg gtttatgata ttctggacca acaggcagta
    24721 gccaatgctc agcaaaaatc agccgcagca gaggatcagg atgccaaccg aaaaaaaaga
    24781 atgcggatta ttcagaaaaa aggcccggaa caaaaggtat tcagtacaaa tccgacagta
    24841 acacctgcca ccgctgaaac aaaacctcaa ccaactgtcc aaccgacatc atctcctaaa
    24901 caagttcaaa aaacaccggc tcaaaagctc ccgaaagcac cccaacctgc ctcaacagaa
    24961 gccattttaa ccgaagaacc ggctcaaaag caggaacaaa agaaaaaaaa tccgcggaac
    25021 agcaaaaaaa ccgctgtcac taaaacttct cctacagaaa ccacagctgt acaacccaca
    25081 gtaaaggaag acgctcccaa acaagcacca atagctgatc aaaaagtaat gcctctgaaa
    25141 aaggtggttt tgttgcccga agtattggaa gaaacacccg aaatggaatt gccacaagaa
    25201 ccacaggtgc agactgaaac tactgatgat accgcagtgg cggcagcaaa aacggagcag
    25261 gtacaaaatc aaaataccaa caagggcaat caacctgtac atcagggcac acaacccaat
    25321 ggcaatcgcg tgaaattcaa tccacggcag ggagaaaaag agaaaaccta cgattttgaa
    25381 gagatactgg aaggaaccgg aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25501 nnnctcactc ccctttttcc cgatgaaaaa ttcaccctca atcggggaaa aaacgacacc
    25561 ctttcaaccc gcattgtcga tcttttcagc ccaataggca aaggtcagcg cggactcatc
    25621 gtagcacaac cgaaaaccgg taaaaccgta ttgctcaaag aaatagccaa tgctattgca
    25681 gccaatcatc ccgaagtgta catgattgta ctgctgatag acgaacgacc ggaagaagta
    25741 acggatatgg cacgcagtgt aaaagccgag gtgatatctt ccactttcga cgaaccagcc
    25801 gaacgccacg taaaagtggc aagtatggtg cacgaaaagg caaaacgcat ggttgagtgt
    25861 ggtcaggatg tggttatcct gctggatagt atcacccgcc tggcacgtgc ttacaatacc
    25921 gtatcaccgg cctctggaaa agtactttcg ggtggtgtgg atgccaatgc acttcacaaa
    25981 ccaaaacgtt tcttcggagc tgcccgtaat atcgaaaacg ggggaagtct caccattatt
    26041 gcaacagccc tgaccgaaac aggttcgaaa atggatgaag taatcttcga agaatttaag
    26101 ggaacgggta atatggaatt gcaacttgac cgtaaattat ccaacaaacg tattttcccg
    26161 gctgtggaca tcatggcttc gagcacgcgc cgcgatgacc tgctgctcga tcaggaaaca
    26221 ctgaaccgca tgtggatact tcgccgacac ctggccgaca tgaactccat ggaagccatg
    26281 gaattcctca aagacagaat tgaaagaact gaaaataatg aggaatttct gatttcgatg
    26341 aatggataaa acccgtttgg atgtagtctg aagtttatca aaatgattta taaggaaaaa
    26401 gcccgtcgta tgcgggcttt tttattttat cacaagacgc taattcaggc ttcgagccac
    26461 tcggcaatga atttttcgtt actggccata aattttggtt tagaaacacg actttcgttc
    26521 ccgggcgcaa caggaaatct gcgatgatcc cagtcgtccg gcatcccaaa atgtttgtac
    26581 gacagccagt tggccgacca ctcggtccag gtgtccatgt gaggtacaaa ttttcggcgc
    26641 tggtttcggg cacttttcag gcttgctgca gctatgcttc gccagaaaaa cagaaagccc
    26701 catttgcgaa actgaagtat atgaccgaat tcgtggcgca acaaatccaa atcatcctcc
    26761 ataccccgcc caacaaaaat gccaatgccg ggaatggtta cggcagctcc acgcttgaaa
    26821 ttaccaacat tcagccgata aatattaatt ccctgaaatt tcatttttta gttcttttca
    26881 tgacccgttc gtacacctca atccaatcgg aaacactcac cttaccggcc aactcaccta
    26941 tcaggtcgta gggtatatcg tccggttttt tgaaacggat acaaccgcgg cccatgtcca
    27001 gtttggtttt gcagtgcttc ggatattcag tctgaaacca ggccagcaac tccggcatag
    27061 ccgatatgct catgtgatac agggcaatgt aatttttctg cgaagccaga ttgatgaatg
    27121 gcaacggaag tttggggttg gattgatagc cggccggata gagactgtgg ggcaccacat
    27181 agccaatcat accatacccg atttcttccc gaaaaccttc aggaagattt tccagcattt
    27241 ttatccgtat tttttgaata atttcccttt tatcgttggg cagctgagcg acatactcgt
    27301 ccggagtggt aaatgttatt tccatttatt gtcttgtgta ttttgtcgtt aaaagtcttt
    27361 tttgcaaata taagtggatt tcacaaaata ccaaagaata taaaatccag aagtgggaaa
    27421 catacgataa taaacgattt aataattgag aatatttgcc tatttttgta agatatttat
    27481 tgcatcgaat ttcagaaact gaaaatcatc aaagcataag tatctattta tttgtcaagg
    27541 atttgtattt ttagataatc cgttatcact tattttacat cgagtattaa tattctgcat
    27601 cacactcaat catgagaaat tttctttcat taatttttct ttttttatcg gcaagttacg
    27661 ttcctgcaca ggggatactg gagtacgaaa gccgaacatt tactcctcac tggtatgtgg
    27721 gtgccaacgt agctgccaac tggtatatgg ccgaaggaaa cgattttctt gcgggaacag
    27781 atggggttcg gttttcgttc aaagacaatt ctggaaccac atttcgtgcc acattgggcc
    27841 ggcggcttac gcccgtcatt ggtatcaggg gctatgctgg catcaataaa tataaatggc
    27901 tcagttctta cacaaataat cctgttgtat cctttaattc ggaatttgcc actgccgatt
    27961 tgaccgtaag cattgtaaat ctgctggaag aatcttattc tctgaaaaaa tatgatttcg
    28021 gcttttttgt tggcaccggt ttcgaattgc gaaataaaat caacacacct gctgtggcag
    28081 tgtcaaacag caacttaacc cccgttgggc gggttggact ggaagggaat tttctgctat
    28141 cgtctaatat tgctttaacc ctgggaggtg aacttaactt tgtaaacgac aacaacaacg
    28201 gactggtagc cggacgcaat tacgaattta ttccggctgt ttctgtcggg cttaattann
    28261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag gaacccgtgt agttcccaca
    28381 tcaatgatta caactccatc ctttaccatg tcagcagtca gaaattcggg ctggccaagt
    28441 gcagcaatga tgatatctgc ctgaagggta aattctttca ggtttttggt gcggctgtga
    28501 caaacagtaa ccgtagcgtc gcccggcgcg gatttctgca tcatcagtga agcaatcggt
    28561 ttacctacga tattactacg gcctatcact acgcagtgct tgccggaagt ctcaatttcg
    28621 taacgtttca gcagttcaat aattccagcg ggagtggcag aaacgaagca cggcatcccg
    28681 attgacatcc ggccaatatt taccgggtga aatccgtcca catccttgcg gtaatcaatg
    28741 gcttctatca ctttttgttc tgaaatatgt ttgggcagag gtagttgcac tataaaacca
    28801 tccacatctt catccttatt cagttcatca atttttgaaa gcagctctgc ttcagttacg
    28861 tcattttcgt aagttatctt tgttgatttg aatccaacct gctcacacga tttgacttta
    28921 cttgccacgt aagtttcact gccaccgtcg tgtccaacaa ttattacagc cagatgaggt
    28981 cttttttttc cggcagcgat cattcctttt acttcttcgg caatttccgc cttaacctgt
    29041 tcggctatta cttttccatc aattaatttc atagttttct gctttgattt tttccactgg
    29101 aataatgaga tttttgtaga atatgttaat aaaaaagccc ctgaaatcat tcggcgatga
    29161 tttatcgggg cttttatttt atcttctttt cattttttgt ttgtgctgtt gcatcgttgc
    29221 cattttcatc atcttactca tctggtcaaa ctgcttgagc aggcggttta cctctacgat
    29281 ggtagtgccg ctaccggctg cgatccggtt ttttcggctc ccattcatta ccgacggatt
    29341 tttgcgttcg tgtggcgtca tggagtgaat tattgcttca attcccttga atgcattatc
    29401 atctatatca atgtctttca actgtttgcc cataccgggt atcattgctg ccaggtcttt
    29461 gatattaccc attttcttaa tctgctggat ttgcgacatg aaatcatcga agtcaaactg
    29521 atttttggca atttttttgc tcaaccggcg ggcttcttct tcgttatatt gttcctgtgc
    29581 tttttccacc agcgaaacga tgtcacccat acccagaata cggtcggcca tacgttcggg
    29641 ataaaagata tccagcgcat ccattttttc gccggtacct acgaacttaa tcggtttatc
    29701 cactaccgaa cggatggaaa gcgctgcacc accacgggta tcaccatcga gttttgtaag
    29761 gataactccg tcaaaattca gacggtcgtt aaattccttg gctgtattta cagcatcctg
    29821 ccccgtcatg gcatccacca caaataatat ttcctgagga tttatcgctt ttttgatggc
    29881 tgcaatttca ttcatcatca gttcatcaac agccagacga cctgccgtat cgacaataac
    29941 cacatcattg ccattcaact tggcttgtcg gatggctgct tcggcaattg ccaccggatt
    30001 tttactggtt tcgtcggagt aaaccggtat atttagttga gcaccaagca ctttcagctg
    30061 ttcgatggca gcaggacgat aaacgtcacc ggcaaccagt aaggggttct tggcgcgttt
    30121 ggttttcagc aggtttgcca gtttaccgga gaaagtcgtt ttaccggaac cctgcagacc
    30181 ggacatcagg ataacagccg gtgatccttt caggtttata tccacactgc ttccgcccat
    30241 gagttgtgtg agctcatcat gtacaatttt caccatcagt tgctgaggct tcagcgaagt
    30301 gagcacattt tgtcccatcg ctttctcctt caccgattcg gtaaatgttt tggcggtttt
    30361 aaagttgacg tcggcatcga gaagcgcctt tcgcacttct ttaagcgttt ccgccacatt
    30421 gatttcggta attttacctt cccctttgag gattttcagc gatcgttcta atctttcact
    30481 taatgactca aacatatact tttctgatat ttaatatatt gcaataattt gtcaattata
    30541 tgacatgcct gttttcaggt tcgcaaagtt agcaaaaaat gacttaaccg gcaataagta
    30601 aaggcactaa tcagttgaaa taaaattatt caatttcatt ttttcaagga tcatactgtt
    30661 ccatttcgat attcgaaaaa ttaatctgat gatgtattca aaataattaa caaatttgga
    30721 caggcatctt aaaaaaaaac tatatttgca tttcaataat tcatccggac ttgatatgaa
    30781 aaaacttctg ccgttgagtc ttatatgcct tttttccggt ttacaggtaa ccgctcaaac
    30841 ggcaataatt caatccgttt tacagaaaat tgaaactacg tatgcacccg acaagcgtgt
    30901 aatagttttt gatgtttcat tgcagggaaa tgaaaatgaa gcggttctaa agggtgaaat
    30961 ttccgaacct gaaattcaca acatcatgat tacaaaaatt aaagaaattt gccccgaaat
    31021 acgtgacagc attcggttac ttcccgataa atcgtttggt gaaaatcagt ggggaatagt
    31081 accgctatcc gccatataca ttagcaaagc acccgatttt ggagcggaaa tcactacaca
    31141 ggcactgatg ggcacaccgg tgagaattct ggaaagaaaa ggcggctggt taagggttca
    31201 gacccctgac aggtacattg ggtggacaag tacggacgat atagagcgga taaaccggaa
    31261 acagttgaat gaatacaatc aattacaaaa agtcattgta acatctcatc atacttttgt
    31321 actgaaaaat cctaataaaa attccgaaat tatcaccgaa gctataatgg gtaatctgat
    31381 ggtggtgaat gagatgaaac aaggcaaaaa atactggtct gtaacccttc cggatggaaa
    31441 aacaggctat gtcaggaaag aagatatccg tccgtttcaa cacggggaaa taaatctgag
    31501 ctccaacagc ataatcaaac tgggtaaaag tttcatgggt ctgccctatt tctggggtgg
    31561 caccaccagt cgcggtatgg attgcagcgg tttcaccaaa ttggtattct ttatgcatgg
    31621 aattatactt ccgcgcgatg cttctcagca atactattgt ggcactgaag tcgatacgag
    31681 tgccggatgg ggaaatctcc
//
LOCUS       DAOPZK010000005        29926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1512_length_29926_cov_24.591041, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000005 DAOPZK010000000
VERSION     DAOPZK010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29926
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_29926_cov_24.591041"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKH68_00600"
     CDS             <1..219
                     /locus_tag="PKH68_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNX11464.1"
                     /translation="LGLVNLQQNKIIKIFTVVSVIFLPPTLIASIYGMNFRVLPELNW
                     AYGYPFSIMLMVVFSAAVLWYFRRKKWL"
     gene            351..683
                     /locus_tag="PKH68_00605"
     CDS             351..683
                     /locus_tag="PKH68_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11465.1"
                     /translation="MEEKSSYQQKVEAQLEEWKADLEQLKAKAKDVTVDLQAEYNEKI
                     KELEPKIAEGKAKLAELVDTADDAWDDVKEGAESIWKQMKETFNNVKEKFDKDDEPKE
                     PEAPKTEE"
     gene            804..2723
                     /locus_tag="PKH68_00610"
     CDS             804..2723
                     /locus_tag="PKH68_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNX11466.1"
                     /translation="MRRNYLIKSILVAMLAVAPVITSFAIRAYPGLIKVKQPDGSTLT
                     IRNHGDEFRHYTTTEDNFVVKQNASGFYTYATVDAQGVITEGARIAKDPATRPDADIK
                     YLVRMPEVRPNLQPSKLQRIKPVQQNTSVMKKAFPVTGSGKSLIILVNFKDKAYVTPT
                     PLTAFTNLLNQPGYSTNGGTGSAKDYFMASSYGKFAPQFDVVGPFDLPSNMSIYGGND
                     TNGDDKNPAQMIVDACAAADATVDFTQYDTDNDGFVDNVFVYYAGYNEAEGGAANTVW
                     PHRWSIFSAGITTGITFDGKKIEDYACTSELQGASGSNMCGIGTFCHEFGHVLGLPDY
                     YDTSGTQSNTLDYWSIMDYGAYSNEGRTPPVYSAYDRFYLGYSSPVEINQPSNVVLLP
                     LYQGTTIPANTDKQSYLLSATTHNLSGTSPNPKEFFIVEYRKTTGWDKYLPGEGMLIW
                     HIDYDQTAWNDNSPNNYTGSSQTLASHMRVYLQPLSGSSTTPGDAFTSGNFTPTTWAG
                     AAIGRNITDIAKTDANISFKLMGGSLTRINLGVIQGQLTFPVIKAGKTNPKNLNIATT
                     GINGNLTVALSGANADYFTVSTTTITQTAANSTAGYDLLVTYKPFAAGSHTAVLSITG
                     GGLPDKVITLTGTAQ"
     gene            complement(2805..3260)
                     /locus_tag="PKH68_00615"
     CDS             complement(2805..3260)
                     /locus_tag="PKH68_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11467.1"
                     /translation="MKKLFPAIFLLFAVAGIAQAQTMDKAIGIRITDGAELSFQNPLG
                     NSTRAELDLGIYGNNTVLLTGIHQWVSGLQEGINWYAGLGPQVGLWNKKDNSGKHFAL
                     GIAGQVGIEYNFDAPVQISLDYRPGWMIIPTGHNYFSWFAVALGLRYRF"
     gene            complement(3331..3795)
                     /locus_tag="PKH68_00620"
     CDS             complement(3331..3795)
                     /locus_tag="PKH68_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11468.1"
                     /translation="MKKLLLAVALLIGVTSFAKAQTMDKAIGLRFTNGAEISFQNPLS
                     NSTRLELDLGLYGLGDHNGFVLSGLHQWVFSLDKGLNWYVGLGGQLGSRSYEKDNVWN
                     SGFGLALAGQIGLEYNFSIPLQVSLDYRPAWYVIPAFGGAYDTVGLGIRYRF"
     gene            complement(3868..5667)
                     /gene="ispG"
                     /locus_tag="PKH68_00625"
     CDS             complement(3868..5667)
                     /gene="ispG"
                     /locus_tag="PKH68_00625"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNX11469.1"
                     /translation="MWKSDPYNYHRRPTDEVKIGTLKMGGANPVRVQSMANTNTNDIE
                     NSVAQCLRIVDAGGELVRFTTQGQKEVESLNIIHAKIRDAGCEVPLVADIHFNANVAD
                     LAARFVEKVRINPGNYVSGVKTDVTSDYTDEEYQLEYEKIRNRFTAFLQICKSHHTAI
                     RIGVNHGSLSERMLNRYGNTPEGMVESCMEFLRICRHENFTNVVISMKASNTVLMMQS
                     VRLLVETMEHEDLHFPVHLGVTEAGDGEDGRIKSAVGIGGLLADGMGDTIRVSLSEEP
                     EAEIPVARLLRDYIAGRENHKTIELKSKKPVGFVPFARRKTTAVRNIGGENLPVVISG
                     LCDHPSLKADYTLIENEIKNQTGEIFCILDPVNFFPANDGLSFLRINYNQLSNAIVEQ
                     LKAKPDIVILLESSHINPVGEQRAFIHTLTAEECTNPVIVVGRYSEDNLEALQIKASA
                     DLGVLFVDGLADGIMIENKGRIPENDILSLSFGILQATRVRVSKTEFISCPGCGRTLF
                     NLQTVVAAIKAKTSHLTGLKIGIMGCIVNGIGEMADADYGYVGAGRGKVSLYKKQSCI
                     ERNVPENEAVDRLVALIKSNGDWKEPDNSINNF"
     gene            5898..7562
                     /gene="sulP"
                     /locus_tag="PKH68_00630"
     CDS             5898..7562
                     /gene="sulP"
                     /locus_tag="PKH68_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HNX11470.1"
                     /translation="MKVFDPKIVFTLKHYTKKQFYSDLMAGVIVGIVALPLAIAFGIA
                     SGVTPEKGLFTAIIAGFIISLLGGSRVQIGGPTGAFIVIVYGIVQQYGLTGLAIATMI
                     AGLMLIAMGLMKLGTVIKFIPYPVVVGFTSGIALTIFATQIKDLFGLQIDKVPADFLH
                     KWVVYAHNISSINWWATLIGVLSIAIIAITPKISKKIPGSLIAIVLMTVVVYVLKHSF
                     GVEGIETIGDRFSINAALPQASKFSIDFDTVRTLLPVAFTIAMLGAIESLLSATVADG
                     VIGSRHNSNTELIAQGVANVITPIFGGIPATGAIARTMTNINNGGRTPVAGIIHAFVL
                     LLILLFLGNLTKHIPMACLAGVLVIVAYNMSEWRTFKSLMRNTKSDVSVLLATFLLTV
                     IFDLTIAIEVGLLLAVVLFIRRISETSTISVFQDEVEAASYVEGGAETDKLSLPKGVE
                     VYEIEGPFFFGVANKFEEIMKQVNDKPKVRIIRMRKVPFIDSTGVHNLENLIKMCNKD
                     KTTVLLSGVNDNVRKVLLQVELDKKIGLENICSNINEAIEKAAKLV"
     gene            7684..7977
                     /locus_tag="PKH68_00635"
     CDS             7684..7977
                     /locus_tag="PKH68_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11471.1"
                     /translation="MENRYASLISEFELRIKKLISEYRILRQENDILKAELSRKQEEL
                     MAAHKDILDLRSDYKILQTTMGMGGSEDERKNSRQHLDKIVREIDKCLMLLND"
     gene            7983..8276
                     /locus_tag="PKH68_00640"
     CDS             7983..8276
                     /locus_tag="PKH68_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNX11472.1"
                     /translation="MADKEMFNINITVNGVRMPLHISRDDEVIYRNAEKLFNRFFRQY
                     SEVYSQRSMEEILTLVAYQFAYIVSKQGMNQDVEPLAEKIQQLNNQLRELMPE"
     gene            8412..9938
                     /gene="rny"
                     /locus_tag="PKH68_00645"
     CDS             8412..9938
                     /gene="rny"
                     /locus_tag="PKH68_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNX11473.1"
                     /translation="MNMEYILIAIAALVLGALGSYLLLGVFNKRARREMEAEAEMLKK
                     NKMIEAKERFIALKAEHEQQVQQRNAKVQVAEVKLQQREMQINQRQSELQKKINETEA
                     QKESLDQQLNALDNKKKEIEHLHRQAQEQLETISGLSAEQAKERLVESLKEEAKTNAL
                     SYINDIMDEAKMTANKEAKKVVIQTIQRVATETAIENSVTVFHIDSDEVKGRIIGREG
                     RNIRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVTDGRIHPARIEEVV
                     NKVRKQVEEEIIETGKRTTIDLGIHGLHPELIRMVGKMKYRSSYGQNLLQHSREVANL
                     CATMASELGLNPKKAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKFKEKPEIFNAIG
                     SHHDEVEMETMLAPIVQVCDAISGARPGARREIVEAYIKRLNDLETLALSYPGVIKTY
                     AIQAGRELRVIVGADKLDDKETELLSFDIAKKIQDEMTYPGQVKITVIRETRAVSYAK
                     "
     gene            10084..10481
                     /gene="ssrA"
                     /locus_tag="PKH68_00650"
     tmRNA           10084..10481
                     /gene="ssrA"
                     /locus_tag="PKH68_00650"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            10871..12040
                     /locus_tag="PKH68_00655"
     CDS             10871..12040
                     /locus_tag="PKH68_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX11474.1"
                     /translation="MKKLFFIVLIIASAITYTSAQTAATTDAAHWSFALKGGLDYFNI
                     YPGGDNITDNGSWGAGAAIEYTINPIVGVGLNLDYLNFNRSSIKGKTIDPSLFTSVNL
                     SNLLYPKRNNAKLNFYANFGGGLSLGSSSDLVLPGGTYKRGASAMTDATNALAYTGAS
                     MEYNVSKVIGIGLDYTYRGYITPVSPYIEYNDANTLMATLRFKLGTGSKTHVRDMTTQ
                     DYYPLPAPVIKQVENPYDDSALKSRLDGLDKAGMDLNNRLKNLEADVKGLKDAAEGAK
                     VSASFQNIEFDFNSDKITKASYPTLDQVAAILNANPTWGKLMINGHTDNVGSDAYNQK
                     LSEARAAAVKKYLAGKGISEAAMSTAGYGESKPIADNATAAGRQNNRRVEFEISK"
     assembly_gap    12394..12493
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12494..13045
                     /locus_tag="PKH68_00660"
     CDS             <12494..13045
                     /locus_tag="PKH68_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX11475.1"
                     /translation="SYAMPKTTASQLAGNLNGFHLGPLVQYNINEKIGIQTGALYNYN
                     NSIITKNSVGTSGTWKQNRVIGQSLDIPLHAVYSVPLADDFYVQIGAGPNFNYALNKY
                     KQTEYYVESKIVDRLTEKGTSIYASPSNFNPLDVQAGLSVGLRYTGVSLRASYDWGIT
                     DRDKTTDGKNRENNIKISIGYTF"
     gene            13203..13874
                     /locus_tag="PKH68_00665"
     CDS             13203..13874
                     /locus_tag="PKH68_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX11476.1"
                     /translation="MKFRLFAILIVILSFIGELPAQIAKKDTIALKKPAYRMEAGYTH
                     INRYGEYVSKTPFHGVRVGATAEYPLKYGFGVQTGLKYSFLFGNKQQLYTAGDSVNYS
                     YTGHSIDIPARLTYTLPIFWGLKIFAYAGPSFNIGLAQTSKVTVTGLPIIEGFPIDIA
                     SGTYNMYNKELNRFSVMLGAGGGIQWKDYRVKGGYDWGLNSISKDKNSPQRLRGWYAA
                     FEIDL"
     gene            13979..15352
                     /locus_tag="PKH68_00670"
     CDS             13979..15352
                     /locus_tag="PKH68_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX11477.1"
                     /translation="MTTTVENKLGTMNVRKLLWMYSLPAIVGAMIASLYNIIDRIFIG
                     QGVGPMAISGLALTFPFMNFLSAFSMLVGAGAASRISIRLGEGDKDRAEKILANALML
                     TFIITGVVIVFSRIFMDPILILFGGSENTLEYAREFMRVIIPGSILSALNFGFNNIMR
                     ASGYPQKAMLTMIICGVINVLLVPLFIYGFHWGIAGAAHATNISYFVGTVWVLTHFMQ
                     KKSVIRFRKRNFQLNKQIIKSILNIGMSPFSMQIASSLVIVLLNIALIKQGGDLAVGA
                     LGIQNSIATLFLMFIIGLNQGIQPILGYNYGAQNYDRMFSTLKTAALIATSVTTFGFL
                     LCTLFPHALVSIFTTDDELKRIAANALRISVIMFPLIGSQVIFTSFFQSIGKAKISML
                     LSLTRQVLFLIPAVLVLPSIFGLNGVWASLPVADFLAVIVTSSTLFFFIRRFEKKNDY
                     IFRINQP"
     gene            15427..16923
                     /locus_tag="PKH68_00675"
     CDS             15427..16923
                     /locus_tag="PKH68_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX11478.1"
                     /translation="MFRIKKLDIYIIKSFLAYFFMTFFICILILMMQFTWKHLAELIG
                     KGVEWKVLAEFFWYTTLQTVPMALPLAILLAALMNFGNLGENFELIAMKSAGISLFRI
                     MRGLIVFIAFICVGAFFFSNNVLPVAQTKLWTLIFSLRQKSPEFDIPEGEFYSGINGI
                     NLYVRHKVPEKRLLKDLMIYDFTNGFDNATVMLADSGRVQFTADNKYLKLTLLNGESF
                     ENLKQQSNNSSANSIPYRRETFSTKEMLIDFDTEFNRYDESILKDQHISKDIVQLTHT
                     IDSVSKIVKTRGNQQANEMIKTEFTNFENRNSVRLNKKDAKKINIDSLFLSLSQEKME
                     EAMMVAQQNVRAKKEKIEFNKVMLEEPTYYVRRHQVEWHRKFTLSFACLIFFFIGAPL
                     GAIIRKGGLGLPVVISVMMFIFYYIIDTSGYKMAREGFWEVYQGMWLSSAVLFPIGLF
                     LTYKAVTDASLFRSEQYMKILDNAVAGFGKLWGKNNKIQHTEPELSTN"
     gene            16940..18166
                     /locus_tag="PKH68_00680"
     CDS             16940..18166
                     /locus_tag="PKH68_00680"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNX11479.1"
                     /translation="MERKFNTIEETIKDFGNGKMVIVVDDEDRENEGDFICAAEKITP
                     EKVNFMLRHGRGVLCAPLIEDRCDELELNMQVEVNTSIHSTPFTISVDKLEGCTTGVS
                     ASDRAATFKALADPASTPETFGRPGHIFPLRARSKGVLRRAGHTEAAVDLARLAGLYP
                     AGALIEIMNEDGSMARLPQLFELADQFKMKIITIADLIAYRIRTESLIEKGDEVDMPT
                     AYGHFKLIPFRQVSNGLEHVVLIKGDWEKDEPVLVRVHSSCMTGDIFGSMRCECGEQL
                     HKAMQLIEKEGKGAIVYMNQEGRGIGLMNKIKAYKLQEEGMDTVDANLHLGFRADERD
                     YGVGAQILREIGVSKIRLMTNNPKKRIGLEAFGLSIVENVPIEVKPNEYNEFYMLTKK
                     DRMGHKLKYVGKDNVK"
     gene            18163..18921
                     /locus_tag="PKH68_00685"
     CDS             18163..18921
                     /locus_tag="PKH68_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX11480.1"
                     /translation="MNRTAIVTGATSGFGRAIALRLAGLGYRLIVTGRRKELLDTLTA
                     EISEKYSVSVLALNFDVRDFKACEEAVNSLPDEFKSIDLLVNNAGLAAGAAPFDQSLL
                     DDFDRMIDTNVKGLLYMTKLIVPGMIERQAGHIVNISSIAGIEVYPSGSVYCASKHAV
                     NAITKGLRIDLVKHGIRVSSISPGMAETEFSIVRYHGDTEKAKAVYNGLTPLNAEDIA
                     DALEFIVTRPAHVSINDIQINPSQQANTYISHRK"
     gene            19089..20912
                     /locus_tag="PKH68_00690"
     CDS             19089..20912
                     /locus_tag="PKH68_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNX11481.1"
                     /translation="MDAPDTMNEMDNLNESLITPEEIQEVTTEPILEVKPEAPVAHDL
                     TKQEICDRMKELIEQNVEQVKEEVESMKQLFYKKSKSEVGEQRAAFIEKGGVETEFIP
                     EPTELEAEFKELLNEFRNNKAKLNAQQEQEKGNNLLKKQHIIEQMKQLVESNDDVSAN
                     VAAFRQLQQDWKNTGPVPPTATNELWKQYNLYQESFWDLIKINNELREYDFKKNLEAK
                     TKLCEAAEMLANEEDVVSAFRQLQKLHENWHELGPVARELREEIWNRFKTASTTINKK
                     HQSHFDDIRKVEDENMALKASLCERIEAIDTTKFSSYKDWDEATKIVLEAQEEWRSVG
                     FAPRKANQKIFDRYRKACDKFFSTKAEFYKSIKSNLTQNLEQKKLLCEKAEAIKDSTD
                     WKEAGDQLVQLQKEWKTIGPVAKKYSDDIWKRFITACDYFFEQKNKNASGQRSVEMQN
                     LDKKKELIAKITGFEKTGNAAESLAALRALAAEWNALGHVPFKEKDKIYKEYRAALDA
                     QFEKLNIDVSQRRLETFRNNLKDMTSKGDNKLYREREKLVRAYEHLKSEIATYENNIG
                     FLSIASKKGDGMLKEMERKIETLKEECKLLEQKISLIDDQM"
     gene            complement(21004..22077)
                     /gene="pdxA"
                     /locus_tag="PKH68_00695"
     CDS             complement(21004..22077)
                     /gene="pdxA"
                     /locus_tag="PKH68_00695"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNX11482.1"
                     /translation="MEPEKIIIGITHGDINGIGYEVILKALSDNRVLDMFVPVIYGSS
                     KLAGFYKKNLDLQGLNLNVINSLEEANSKRINIINCVDEELKVELGQSTAEAGKAAYE
                     ALEKATEDLKKGRLHAIVTAPINKKNIQSADFHFPGHTEYLETKFGEQSSALMMLVND
                     FMRVAVVTGHIPVSNVASVITEDLIVEKLKILNSSLKKDFSIVKPRIAVLGLNPHAGD
                     DGVIGNEEQNVIIPALKKADSQGILCFGPYPADGFFGSGNFRNFDAILAMYHDQGLIA
                     FKTLSMESGVNYTAGLSVIRTSPAHGTAYTLAGQNKASEQSFLEALYLACDIYKNRLE
                     YIAMNANPLVIEPKRENHKRNIE"
     gene            complement(22088..24967)
                     /locus_tag="PKH68_00700"
     CDS             complement(22088..24967)
                     /locus_tag="PKH68_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HNX11483.1"
                     /translation="MNDTIIEITEENDSTGLSTVVTDTITTKEKYTEEVDTTVIPTEK
                     PPTLPIKQKFVIPRTDRPLTKNISSPFSSTKGLDNRHIALWQSHGLYYKQSKEKWNWQ
                     RANLFETREDLYTQSYILPLLVPMLENAGATVLLPRERDIQRIELIVDNDKSSPDCSF
                     RTTTGNFTWHKGVGSGFAHFKENYRDGENPFKTGTFIQANTVGHDEPSSTAEWHFSVP
                     KSGSYAVYISYKTVPNSSNEAIYTVYHAGGETRYAVNQTMGGGTWIYLGHFTFHKNQP
                     QQSKIVLTNTSTDIHKLITADAVKVGGGLGNISRKRSDLVTKKIIKIPIKSKKKKGKK
                     RRIKYKTKTITTVKHFDTYSTSGMPRFTEGARYWLQWAGVPDSIYSRTKGENDYSDDF
                     QSRGFWVNYLAGGSVSAPGYKGLNIPLDMAFALHTDAGKTSDSIIGTLAIHTVKTKTG
                     AVKYNNGISRWVARDLTDSVQTQVVRDVQQLYDKDWVRRQLWNRNYSESRVPEVPSVL
                     LELLSHQNFPDMQYGLDPRFRFTASRAMYKGILRYLASTNNYEYKVQPLPVEAFCADF
                     TDSTEVELRWKPVDDPLEPTAKPTGYLVYTRMEDGGFDNGEYVNSTRFYKKIERNKIY
                     SFKVEAVNEGGRSFPSEILSVCKTKDDANVVLIVNAFNRISAPASFVSSDVSGFNNSW
                     DAGVPYINDYKFTGVQTEFRKNRPYRSDENPGHGASEEGWEGKVIAGNTFDYPYLHGE
                     SIRKAGFSFVSCSEKSVTDGDIKLYCYPFVDLILGKEREWTSLNKEATQPVFRTFTPE
                     MQQAIMQYTDSGGNLLVSGSNIISDLVLSKNSTDRDRSFVENTLKIKWMSDKINDLHK
                     VNFNNNEINHFKDLNVSFHASPNEESYYVEAPDIITPNGKFAYQICKFEENNQGAGIV
                     YDGNYRICALGFPFETIRDDHDRNKLMKYVLNFFSDTNRNIISYK"
     gene            complement(25102..26142)
                     /gene="rlmN"
                     /locus_tag="PKH68_00705"
     CDS             complement(25102..26142)
                     /gene="rlmN"
                     /locus_tag="PKH68_00705"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNX11484.1"
                     /translation="MRKQALIGKSIDELKEIAAELSMPSFTAKQIAEWIYKKKIGSID
                     LMSNISVKNREILSEKYEVGSYEPIQTEVSDDGTKKFLFKTEMGHYIETVYIPDEDRA
                     TLCVSIQVGCKMNCSFCMTGKQGFSAHLSPADVINQILSVPDSDKLTNIVFMGMGEPM
                     DNLLTLLKSLEIITANWGFAWSPRRITVSTIGILPGMKVFLEKSDCHLAISLHSPFSE
                     EREKLIPMEKVYPIEKVIAELRKHDFRHQRRLSFEYTMFDGLNDSIRHAVELAKMLKG
                     LDCRLNLIRFHEIPGIELKSSSPEKMDFFRNYLNNNNIITTIRRSRGEDVHAACGMLS
                     TKEKLQNHDGSH"
     gene            complement(26256..28361)
                     /locus_tag="PKH68_00710"
     CDS             complement(26256..28361)
                     /locus_tag="PKH68_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNX11485.1"
                     /translation="MATLDKIRSKGVLLAVIVGTALLAFIIGDFLNSGSTLFHQARQN
                     VAEIAGEKINISEFSAAIEQMTNVYKIETGQSDFTEEQNAQLRTSVWDNMVNERILAS
                     EAKKLGLSVSKDELTDRLIGKNIHPLIMQRRVFMDEKGQFSPANLLKFYNNIFGASNG
                     DAEQLKEAKNYWLFWEKAVKNAILQEKYAALISKSVGSNTIEAKYNYDARKTSVDVNY
                     VMQPYFAVSDSAIKVTDAEIKERYNKEKENFKQEANRSINYVAFDVVPLQEDFKEAEE
                     WIKKVSEEFKTTTDVAAVVNSESDISYDGRLYSEKSVPASLKDFAFGNATGAIYGPVF
                     QNNTYTMARVMESGIMQSDSVKLRHIFLAGNDEKKADSIIGALKSGSTFADMAMKYSA
                     VKETAANGGEIGWITEGSVGKEITGPAFSKPTNEIFKVTSAQGIQIMQATEKTPARRK
                     VKLAILERKVTPSSQSYSKIYNGAKEFAANSTSLEKFEANAKQKGYVINPAIGLLKNS
                     DRVNAVPQSRQVVRWAFENKKGTVSDVFDCGTQFIVATTTEINEEGYKTVAQASAQLK
                     AEIIKDKKADLMIKNLSTLLAKYPTLETLAPALNTEVKTAPAVNFAGYQLGEAGFEPY
                     VIGKASVLPTGKVSAPLKGNAGVYVILPGARVADNAPFNAKMESAQLDSRMAYSLPYT
                     LMEKLKEKYKIVDNRSNFY"
     gene            complement(28511..29764)
                     /locus_tag="PKH68_00715"
     CDS             complement(28511..29764)
                     /locus_tag="PKH68_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNX11486.1"
                     /translation="MIYVSNIIISLICLAFFSGMEVAFISSNKLRFELDKKYNNLTSS
                     IISIFFKNSQQFISTMLVGKFLSMVVFGLLITESLTFAFEGALSPFISILLQITIATV
                     VIIIVGEFLPRAFFKINPNMWLRLFSWVLLFFYIVLYPISWFSSWASSKFFSLFKISL
                     PASTEENVFSRIDLNYLLQENLDTKNDQKEMEHEVKIFQKALDFSTVKLRDCFVPRTE
                     IVALEYGVDVNILKQTFIETGLSKIIIYKGDIDNITGYIHSSEMFEHRKDWTKYIRQI
                     PIVPENMAAQKLMKLFMSEKKSIAVVVDEFGGTAGIVTLEDIMEEIFGDIEDEHDIRG
                     YTAEKTGENEYLFAGRLEVEEVNKLFNLNIPVSDDYTTIAGYILHIHQHFPKLNEIIR
                     IENFTFKCVKVTNNRIEMVKLLVNN"
BASE COUNT         8774 a         6126 c         6312 g         8614 t
ORIGIN      
        1 ctcggtcttg taaacctgca gcaaaataaa atcatcaaga tatttaccgt ggtgtcggta
       61 attttcctgc cgccaacgct cattgccagt atctacggca tgaacttccg tgtattgccc
      121 gagctcaatt gggcctacgg atatcccttc tccattatgc tgatggtagt gttttcggca
      181 gccgtattgt ggtacttccg gcgaaaaaaa tggctgtagt gactgtaaat ggatctgttt
      241 ttgttctctc aaatatttaa taaatttaac cccaaattgc tgacctttac caaaaccttt
      301 agtatctttg ttatatagta ttcgttatta aaaaataata attttaactt atggaagaaa
      361 aatcatctta tcaacaaaaa gtggaagctc agctcgaaga gtggaaagct gatctggaac
      421 agttaaaagc aaaggcaaaa gacgtaaccg ttgatttgca ggctgaatat aacgagaaaa
      481 tcaaggaact tgaacccaaa attgcagaag gtaaagcaaa gttggcggaa ttggtagata
      541 ctgccgacga tgcctgggat gatgtaaaag aaggagccga gtcaatctgg aaacaaatga
      601 aagaaacttt caataatgtg aaagaaaaat tcgacaagga cgatgaaccc aaagaacccg
      661 aagcacctaa aactgaagaa taataaaaaa gctgtctgaa aagacagctt tttttaattc
      721 tgattgattt ttaatatatt gggtatttca gaattcaaac agaagttata aatttgcacc
      781 ctttatatca aaaaaaaaca taaatgagaa gaaattacct gattaaatca attttggttg
      841 caatgctggc agttgcacct gttataacct cctttgccat acgtgcttac ccggggttaa
      901 tcaaagtcaa acaacctgat ggctcaactc tcaccataag aaatcacggg gatgaatttc
      961 gtcattacac cactaccgaa gataactttg tcgtaaagca aaacgcctcc ggattttata
     1021 cctatgccac tgtggatgct cagggagtaa taactgaagg agcaagaatt gccaaagacc
     1081 ctgcgacaag accggatgcc gacatcaagt accttgttcg tatgcccgaa gtgcgcccga
     1141 acctgcagcc ctcaaaattg caacgaataa aaccggtaca gcaaaatacg agtgtgatga
     1201 aaaaagcatt cccggttacc ggttcgggta agtcgctgat aattctggtg aatttcaaag
     1261 ataaagccta tgtaactcct acacctctaa cagcatttac caatttgctc aaccagcccg
     1321 gctatagtac aaacggggga accggttcag caaaagacta ttttatggcc agttcgtatg
     1381 ggaaatttgc tccccagttt gatgtggtag gaccttttga cttaccttcg aatatgtcca
     1441 tatacggagg gaatgacacc aatggtgatg ataaaaatcc tgctcaaatg attgtggatg
     1501 cttgtgctgc agctgatgca acagtggatt ttacgcagta tgataccgat aacgatggat
     1561 ttgtggataa cgtatttgtt tattatgccg gatataatga agccgaaggt ggagcagcca
     1621 atactgtctg gcctcaccgg tggagcattt tttcagctgg tattacaacc ggtatcacat
     1681 tcgacggcaa aaaaattgaa gattatgcct gtacatccga attacaaggt gcttccggat
     1741 ctaatatgtg cggaatagga actttctgcc atgaattcgg tcatgtgctg gggctgcccg
     1801 actactatga cacttccggt acacagtcaa ataccctgga ttactggagt attatggact
     1861 atggagcata ttccaacgaa gggcgcactc cgcctgtgta ctctgcctac gatcggtttt
     1921 acctcggata ttcatcaccg gttgaaatta atcaaccctc taatgttgtt ttactgcctc
     1981 tttatcaggg tacaactatt ccggccaata ccgacaaaca gtcatattta ctctcagcca
     2041 ccactcacaa tctgagtgga acttcaccca atcctaaaga atttttcatc gttgaatacc
     2101 gcaaaacgac aggttgggat aaatatcttc ccggcgaagg aatgctcatc tggcacatcg
     2161 attatgatca gacagcctgg aatgataatt caccaaacaa ttatacaggt tcatcacaaa
     2221 ccctggcaag tcacatgagg gtatatttac agccattatc cggttcttcc accaccccgg
     2281 gcgatgcatt cacatccggt aactttaccc ctacaacctg ggcaggtgct gccattggca
     2341 gaaatataac cgatatagct aaaacggatg caaacatatc tttcaagttg atgggtggat
     2401 cacttacccg gattaacctg ggagtaatac agggtcaact gactttcccg gttattaaag
     2461 caggcaaaac aaacccaaaa aacctcaata ttgccactac cggtatcaat ggaaatctta
     2521 ctgttgccct ttcaggggct aatgcagact attttaccgt gagtacaaca accattacac
     2581 aaacagcagc taattctaca gccggctacg atttgctggt tacctacaaa cccttcgctg
     2641 ccggcagtca tactgctgtg ctttcaatca ccggcggcgg actgcccgat aaagttataa
     2701 ccctcacggg aacagctcaa taaaataatt tagtcataaa aaaagccctg gagaatgatc
     2761 tcagggcttt ttttaatgag tgtaagaatg ggttttatat caaatcaaaa acggtatctc
     2821 aatcctaagg caacggcaaa ccatgaaaag taattgtgtc ctgtggggat aatcatccag
     2881 ccgggacgat aatctaacga aatctgaacc ggtgcatcga aattatattc aatacccacc
     2941 tgaccggcaa ttcctaatgc aaagtgtttg ccactattgt ctttcttgtt ccaaagtcct
     3001 acctgtggtc ccagacctgc ataccagttt attccttcct gtagacccga tacccattga
     3061 tgaattccgg ttagtaaaac cgtattattc ccataaatac ccaaatccag ttcagcacgg
     3121 gtggaattac ccagcggatt ctgaaaggaa agttcagcgc cgtcggttat acggatacca
     3181 atggctttgt ccatggtttg agcctgagca atacctgcta cagcaaaaag aagaaaaatt
     3241 gccggaaata attttttcat agttctgtgt tattttaaat ttggttgagt taaattattc
     3301 aaataacggt attacccgga aaaacgggtt ttagaatctg taacgaattc ccaatcccac
     3361 cgtgtcataa gctcctccga atgcaggaat tacgtaccag gccggacggt aatcaagtga
     3421 tacctgcaat ggaatagaga aattgtattc aagaccaatt tgtccggcaa gagccaggcc
     3481 aaaaccggaa ttccagacat tatctttttc gtacgaacgg gaacctaatt gtccgccaag
     3541 tcctacatac cagttaagtc ctttgtcaag actgaaaacc cattgatgta aacccgagag
     3601 aacaaatccg ttatgatcac caagtccgta aagacctaaa tcaagttcca gtcgggtgct
     3661 gttactcaat ggattctgaa atgatatttc agcaccatta gtaaaacgaa gaccgatggc
     3721 cttgtccatt gtttgagctt ttgcaaaact tgttacgcct atcaataggg caacagctaa
     3781 taatagtttt ttcatacttt ctgttttttt tgttatctat gtgttaagtg ttgatacatt
     3841 catttaacgt taaatttttg gttttgttca aaaattgtta attgaattat cgggttcttt
     3901 ccagtcgccg ttacttttaa tgagtgctac caatctatct acagcttcgt tttcgggtac
     3961 atttctttcg atgcagcttt gtttcttgta caagctcacc tttccacgtc ccgcacctac
     4021 gtagccgtaa tcagcatcag ccatttcccc gatgccgttt actatgcagc ccatgatccc
     4081 aattttgaga ccggtgagat gcgatgtttt tgccttgatg gcagctacta cagtttgaag
     4141 gttgaacagt gttcggccac aacccggaca ggagataaat tccgttttgc tcacacgtac
     4201 cctggtagcc tggagaatgc cgaatgagag tgacagaata tcattttcag gaattcttcc
     4261 tttattctct atcataattc cgtcggccag cccatccaca aagagtacgc ccaaatcagc
     4321 cgaagccttt atttgaagcg cctccaggtt atcttccgag tagcgtccaa cgactatgac
     4381 aggatttgtg cattcctcgg ccgtcagcgt gtgtataaaa gcccgttgct cgcctacagg
     4441 gttaatgtgg ctcgattcga gtaaaatgac gatatccggt ttcgctttca gttgctcaac
     4501 gattgcattg ctgagctggt tgtaatttat tcgcagaaat gaaaggccat cattagccgg
     4561 aaaaaaatta accggatcta aaatgcaaaa tatctcaccc gtttggtttt ttatttcatt
     4621 ttcaatcagt gtgtaatcgg ctttgagaga aggatggtca cacaaacctg agataacgac
     4681 agggagattt tcgccaccga tatttctcac agcagtagtt tttcgtcggg caaatggaac
     4741 aaaacctacc ggtttttttg atttaagttc tattgtttta tgattttctc ttccggcaat
     4801 atagtctcta agcaatctgg caaccggaat ttcggcttca ggttcctcgc tgagcgaaac
     4861 ccggatggtg tcgcccatac catcagcaag taatccgcca atacctacgg ctgattttat
     4921 gcggccgtct tctccgtcgc ctgcctcggt aactcccaga tgaacaggga aatgcagatc
     4981 ttcatgctcc atcgtttcca ccagcagtct caccgattgc atcatcagca ccgtgttcga
     5041 tgctttcatc gaaatcacta cattggtgaa attttcatgc cggcaaatcc ggagaaattc
     5101 catgcaagac tctaccatgc cttcgggcgt gtttccatag cggttgagca tacgttcact
     5161 cagcgacccg tgatttacgc ctatacggat ggctgtatgg tgcgatttac aaatctgaag
     5221 aaaagcggtg aaccggtttc taattttttc atactccagc tgatattcct cgtcagtata
     5281 atcggaggtc acatcggttt ttaccccgct tacatagttt cccgggttta tccgtacttt
     5341 ttccacaaag cgggctgcca gatcggctac atttgcatta aaatgaatat cggccacgag
     5401 tggaacctca cagccggcat cccgtatttt agcatggata atattcaaac tctccacttc
     5461 tttttgcccc tgagtggtaa aacgcaccaa ctcaccgccg gcatccacta tccggagaca
     5521 ttgtgcaacc gaattttcaa tatcattagt attggtgtta gccattgact gaactctgac
     5581 cggattggcg cctcccattt taagagttcc gatttttact tcatccgttg gccgacgatg
     5641 gtaattatag gggtctgatt tccacatagt gtgagagttt aaatgcatta ttaatctaat
     5701 gacaaattta gtactttatt gccttaggta aataaaattt tatgagaaat aacaaaattg
     5761 attacctttg caaccgattt gatgcaatca gaaaaaaaat gaactgtcca aaagtaaaac
     5821 tataaaaaca ccaaaaaggt ccttttcgtt taactttgag gacattttct ttttaaaaac
     5881 attaaactta aaaagatatg aaagtttttg atccaaaaat tgttttcacc ctaaaacatt
     5941 ataccaaaaa acagttttat tcagatttga tggcgggcgt aatagttggt atagtagccc
     6001 ttcccctggc cattgcattt ggtatagcat ccggggttac gcccgaaaaa ggattgttca
     6061 cagccataat tgcaggtttt attatatcgc tgctgggtgg cagccgggta cagataggcg
     6121 ggcctacggg tgcatttatt gtaattgtgt atggcattgt tcagcaatac ggacttaccg
     6181 gattggctat tgctaccatg atagctggat tgatgctgat agctatgggg ctgatgaaac
     6241 ttggaactgt tatcaaattc ataccctatc ccgtggtggt gggatttacg agcggtattg
     6301 ccctgacaat ttttgcaact caaattaaag atttgtttgg attacaaata gataaggttc
     6361 cggcagattt tttacacaaa tgggtggttt acgcacacaa tatttcttcc attaactggt
     6421 gggcaacgct gattggtgtt ttatcaatag cgataatcgc aattacacct aaaatctcaa
     6481 aaaaaattcc cggctcactg atagctattg tacttatgac cgttgttgtt tatgtgctta
     6541 aacatagttt tggagtcgag ggtatcgaaa ccatcggtga tcgtttttct atcaatgccg
     6601 ctttacctca ggcaagcaaa ttcagtattg atttcgatac ggttcgaacc ttactgccgg
     6661 ttgcttttac cattgccatg ctgggggcta ttgagtcgct gctttctgct actgttgccg
     6721 atggggtgat tggcagccgg cacaattcca ataccgaact cattgctcag ggggtggcca
     6781 atgtaattac cccgattttc ggtggcatac cggcaaccgg agcaattgcc cgtaccatga
     6841 ccaatatcaa caacggaggt cgtactccgg ttgcaggcat tattcacgca ttcgttctgt
     6901 tactgatact actattcctt ggtaacctta caaaacatat tccaatggct tgtctggccg
     6961 gtgtactggt gattgttgct tacaatatga gcgagtggcg taccttcaaa tcattaatgc
     7021 gtaataccaa atcggatgtg agcgtgctgc ttgccacttt cctgctgacc gttattttcg
     7081 acctgaccat cgctatcgaa gtgggactgc tgctggcggt agtactcttt atccgccgga
     7141 tatccgaaac ttctaccatt tcagtgtttc aggacgaagt tgaagctgcc agctatgtgg
     7201 aaggcggcgc tgagactgac aaattgtcac tgccaaaagg tgtggaagtg tacgaaatag
     7261 aagggccatt tttctttggt gtggccaata aatttgaaga aataatgaaa caggtgaatg
     7321 ataaacccaa agtaaggatt atacggatga gaaaagttcc ctttatcgat tccacaggag
     7381 tacacaacct tgagaatctg ataaaaatgt gtaacaagga taaaaccact gtattgcttt
     7441 cgggagtgaa tgataatgta agaaaagtgc tgttgcaggt tgaactcgat aaaaaaatag
     7501 gactcgaaaa tatctgcagc aatatcaatg aagcgataga aaaagcagcg aagttggttt
     7561 gaggtttaca atctgtgtaa cagtagaaaa atgcttcgtt tctcaactat ttctataaaa
     7621 aaagagtaaa aagtttttta tttcaatgca aaatcttatt tttgtgaaga ttttagtttg
     7681 aaaatggaaa atcgatacgc aagtttaatc agtgaatttg agctaagaat aaaaaaactg
     7741 atttcggaat accggatttt gcgccaggag aacgatatcc tgaaagcaga actcagtaga
     7801 aaacaggaag aattaatggc agctcacaaa gatattctgg atttgcgcag tgattataaa
     7861 atcttgcaaa ctactatggg aatgggcggt tcggaagatg aacgcaaaaa ttcccgacag
     7921 cacctggata aaatagtgcg ggagattgat aaatgtttga tgcttctcaa tgattgatat
     7981 gtatggcaga caaagaaatg tttaatataa atattactgt caacggggtt agaatgcctt
     8041 tacacattag tcgcgacgat gaagtaatat atagaaatgc cgagaaattg tttaataggt
     8101 ttttcaggca atacagtgaa gtgtacagcc agcggagcat ggaggaaata ctgacgttag
     8161 tggcttatca gtttgcatat attgtttcaa aacaaggcat gaatcaggat gtagagccgc
     8221 tggccgaaaa aattcaacag ctcaacaacc aactcaggga attaatgccc gaataaccgg
     8281 acattaatta actgaaaaac atttatttac cgcattaatt gtttatttgt tgtccgcctg
     8341 taccggatag taagttggca attgaatgcg tttttttatt ttaaaatgat ataatacact
     8401 taattaattt aataaacatg gaatatatac ttatagccat agcagctctt gtgttgggag
     8461 ctttgggcag ctacctgctg ctcggagtgt tcaacaagag agcacggcgt gaaatggaag
     8521 ccgaagcaga aatgcttaag aaaaacaaaa tgattgaagc aaaagaacgc ttcattgccc
     8581 ttaaggcgga acatgaacaa caggttcagc aacgcaatgc caaagttcag gtagccgaag
     8641 tcaagcttca gcaacgcgaa atgcaaatta atcaacgtca aagtgagttg caaaaaaaaa
     8701 taaatgaaac tgaagcccaa aaagaaagcc ttgatcaaca actgaatgcc ctggataaca
     8761 aaaaaaagga aattgaacat ctgcatcgac aggcacagga gcaacttgaa accatatccg
     8821 gactttctgc cgaacaggct aaagaacgat tggtggaatc tttgaaggaa gaagcgaaaa
     8881 caaacgcctt gtcctatatc aatgatataa tggatgaagc caaaatgacc gcaaacaaag
     8941 aggctaaaaa agtggtgatt caaaccattc aacgggttgc aaccgaaaca gccatcgaaa
     9001 actcagttac ggtatttcac atcgattccg atgaagtgaa aggtcgcatt attggtcgcg
     9061 aaggacgaaa tatacgggca ctcgaagctg ccaccggggt ggaaatcatt gtggacgata
     9121 ctccggaagc cattgtgctt tcagcctttg atccggttcg ccgcgaaata gcacggcttt
     9181 cgctgcatca actcgtcaca gacggacgca ttcaccctgc acgtatcgaa gaggtggtaa
     9241 ataaagtgcg taagcaagtg gaagaagaaa ttatcgaaac cggaaaacgc actaccatag
     9301 acctcggtat tcacggactt caccccgaac ttatccgtat ggtcgggaaa atgaaatacc
     9361 gctcctctta tgggcagaac ttactgcaac actcccgtga agttgccaat ctttgcgcta
     9421 ccatggcatc agaactcgga ctaaatccca aaaaagccaa acgcgccgga ttactccacg
     9481 atattggcaa agtgcccgac gacgaaccgg aattgccaca cgccattctg ggtatgaaac
     9541 ttgccgaaaa attcaaggaa aaacccgaaa ttttcaatgc tatcggttcg caccacgacg
     9601 aagtggaaat ggaaacgatg ctcgcaccta ttgtacaggt gtgtgatgcc atatccggtg
     9661 cacgtcccgg tgcacgccgc gaaattgtag aggcatatat caagcgcctg aacgaccttg
     9721 aaacactggc tctatcatat cccggcgtaa taaaaaccta tgccattcag gccggacgcg
     9781 agctgcgcgt aatagtggga gccgataagc tggacgacaa ggaaaccgaa ttgttgtcat
     9841 ttgacattgc caaaaagata caggacgaaa tgacatatcc cggacaggtc aaaattacgg
     9901 tgatccgtga aacccgggct gtgagctatg caaaatagtt ttcgcggtga aatgtagttc
     9961 attatcagaa aaatacaaat atgttcggcc attgctgaac atatttttta aaagattgtt
    10021 ataatcttta gaagttttgg cacagaaatt gttaaattaa taatacacca aaaaagcgtt
    10081 tttggggctg cttggttttg acagcaggta gaaaagatat gtaagcatgt cgagcgtcgg
    10141 aatgccggct cgttaatccc ggatttcaac attctaactg gcgaaaacaa ttacgctctt
    10201 gctgcttaat cgaataaagt agatttagct tcatccaggc accaggtgcc aggacgggac
    10261 atctctcaaa tgccgtcgct ccgaggcgat ttgatacaga ggtgcggata aatcggggat
    10321 agtcacttat agccttgtgt gagtgatgaa gtaacaaagg ataagataat ggttggtggc
    10381 ttcggtcttg ccattgtacg aaaaactaag cgaagataaa catgtagaaa gcttatagtt
    10441 tccttgtttg gacgagggtt cgactccctc cagctccact cattttttca aaagaatcaa
    10501 cattcgctga aacaaagaac cggttaattt gtttctcttt tgttgcattc ttattcacag
    10561 ccaataatag tgcagtgact taacttaatg gcaggtttat tcaactcttt ctgttcatca
    10621 ggtttataac atacactatt agcactcggt aacacgattt acttaatgcc ttaatctgat
    10681 ttattaacca aaaaattatc gataaaacct aatggttcta tcaaaaaggc tcatttccga
    10741 cgatttttaa ttaaaatata aaatatttgc tcaaaaaagt atattagtta aaaataagtt
    10801 gtatttttgt aaattataaa aaacgaacga aactaaattt atttaactgc taataaaaaa
    10861 caagtaaaaa atgaaaaaac tattctttat cgtattgata atagcaagtg ctattacgta
    10921 cacatctgct caaactgcag ctactactga tgcagctcat tggtcatttg ccctgaaagg
    10981 tggacttgat tatttcaaca tctatcccgg aggtgacaac attactgata atggaagctg
    11041 gggagccggt gcagcaatcg aatacaccat caaccctatc gttggtgtgg gactgaacct
    11101 ggactactta aactttaatc gttcttctat caaaggaaaa acaatcgacc caagtttatt
    11161 tacttcggta aatctttcca atctgttgta tccaaaacgt aataatgcaa aactgaactt
    11221 ctacgcaaac tttggtggtg gtcttagttt aggcagttct tctgatctcg tattgccggg
    11281 tggaacctat aaaagaggtg cttctgccat gacagatgca acaaatgcat tggcttatac
    11341 cggagcttca atggaatata atgttagcaa agttatcgga attggcctgg attataccta
    11401 tcgtggttat atcacccccg ttagccctta cattgaatac aacgacgcta atacgttgat
    11461 ggctaccctg cgtttcaaac ttggaacagg aagcaaaact cacgttcgcg acatgactac
    11521 tcaggattac tatccacttc ctgctcctgt tatcaaacag gttgaaaatc cttacgatga
    11581 ctcagctctc aaaagccgcc tcgatggttt ggataaagcc ggaatggacc ttaataaccg
    11641 cctgaaaaat ctggaagccg atgtaaaagg actgaaagac gcagctgaag gtgctaaagt
    11701 aagtgcatct ttccagaata tcgaatttga tttcaattct gacaaaatca caaaagcttc
    11761 ttatcctaca ctggatcagg tagctgctat cctcaacgct aacccaacat ggggaaaact
    11821 gatgatcaat ggtcataccg ataatgttgg ttcggatgct tacaaccaga aactgtcaga
    11881 agcacgtgct gctgccgtta aaaaatatct tgccggtaaa ggcatttctg aagcagctat
    11941 gtcaacagca ggttatggcg aaagcaaacc aatagctgac aatgctactg cagccggtcg
    12001 tcagaacaac cgtcgcgtag aattcgaaat ttcgaaataa gaaataaacg aataaatatt
    12061 agtgaggatg tgcgaaagta catcctcatt tgttttaaat gcagataggt tgaaagaaat
    12121 gtaaactgtt taataatgaa ttggtaatga gcagataatc aagaggtaaa atttattaat
    12181 ctcagaaatc aggaaagatc ttattagtgt ggcacagtat ttgcaatctt aaacctatac
    12241 gttttaattt tattgaaaaa tttatattca aacattttaa tagtaataac taaattagaa
    12301 attaatgaaa acaattaaat tatcattagt agccttagct acagtattaa cgatgggtgc
    12361 atcagcacag gtaggtattc aggccggata cagnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnagttatg ccatgcccaa aaccacagcc tcgcaactgg ccggcaatct
    12541 taacggattt cacctcggac cgctcgtaca atacaatatc aacgaaaaaa taggtatcca
    12601 gaccggtgct ttatacaact acaacaattc cattattact aaaaattctg tagggaccag
    12661 tggcacctgg aaacaaaacc gcgtcattgg tcagagtctc gacatacccc tgcacgcagt
    12721 ctattccgtg cccctggccg atgatttcta tgtgcagata ggtgccggtc ccaatttcaa
    12781 ttatgctctg aacaagtata aacaaactga gtattatgtt gaaagtaaaa ttgttgaccg
    12841 cctgacggag aagggaacaa gcatttacgc ttctccgtcc aacttcaatc ctcttgatgt
    12901 tcaggcagga ctaagtgtgg gtctccggta caccggggta agcctcagag caagttacga
    12961 ctggggtata acggacaggg acaaaactac cgatggaaaa aacagggaaa acaatattaa
    13021 aatctcaatt ggatatactt tttgatgatt atcagtaatc tttgttcaat taaaatcatg
    13081 gacgaacgct aacacggctc gtcctttttg tgttaataaa cgttttgatt tagaaggtat
    13141 gtaggtattt atccttattt ttgcagaaat aaacagtgaa agattattct aaaaacttat
    13201 ttatgaaatt ccgtcttttt gctattctta tagttatcct gtcttttatc ggagaacttc
    13261 cggcacaaat cgctaaaaaa gataccatcg cactgaaaaa accggcatac cgaatggaag
    13321 ccggttacac gcacatcaac cggtacggtg agtatgtgag caagacaccg tttcatggtg
    13381 tccgtgtggg tgcaacagcc gaatacccgc tgaaatatgg atttggagtt caaacgggac
    13441 tgaaatactc atttttgttc ggcaataaac aacaacttta cacagcaggt gattcggtca
    13501 attactcata caccggtcac tctattgata ttcctgccag actgacctat acactgccga
    13561 tattctgggg cctaaaaata tttgcctatg ccggacccag tttcaatatc gggttggctc
    13621 agacatccaa agtaactgtg acgggattac ccatcatcga aggttttccc attgatattg
    13681 cttcaggtac ctacaatatg tataacaaag aacttaaccg cttttcagtc atgttgggtg
    13741 ccggaggtgg tattcagtgg aaagattacc gcgtaaaagg tggttacgac tggggcttga
    13801 acagtatcag caaggataaa aattcaccac aacgtctaag aggctggtat gctgcttttg
    13861 agattgattt ataagtaaaa gaacgtattt acagagaaac cggttttaca atgtaaaacc
    13921 gttttttgtt taggaagaat ctgttccggg atattttaga gatgaataga aatttaagat
    13981 gacaacaaca gttgaaaata agttgggcac catgaatgtc cgcaaattat tgtggatgta
    14041 ttccttgccg gcaattgtgg gagccatgat tgcttcgctg tacaacatta ttgaccgtat
    14101 attcattggg cagggagtgg gtccgatggc catttcgggg ttggcgctca ccttcccgtt
    14161 tatgaatttt ctgtcggctt tcagcatgct ggtgggtgcg ggagcagcat cgcgtatttc
    14221 catccggttg ggcgaaggtg ataaagaccg tgccgaaaag atactggcta atgccctgat
    14281 gcttaccttc attattaccg gagtggttat tgtattctca agaattttca tggatccaat
    14341 acttatactg ttcggaggaa gtgaaaacac actcgaatat gcccgtgaat ttatgcgggt
    14401 aattattccc ggtagcattt tatctgcttt gaattttgga tttaacaata tcatgcgggc
    14461 ttcaggttat cctcaaaaag caatgcttac gatgattata tgcggggtga taaatgtatt
    14521 gctggttccg ttgttcattt atggatttca ctggggaata gccggagcgg ctcatgcaac
    14581 caatatatct tattttgtgg gaacggtgtg ggtgctgaca cattttatgc agaaaaaatc
    14641 ggtcattcgt ttccgaaaaa ggaattttca gctgaacaaa caaataataa aatctattct
    14701 gaatataggc atgtcgccat tcagtatgca gatcgcttcc agtctggtga ttgtattact
    14761 caatatcgca ttaataaaac aaggaggcga tttggcggtt ggagcgcttg gcattcaaaa
    14821 cagcattgct acgttgtttc taatgtttat tatcggtctc aatcagggca ttcagccaat
    14881 tcttggatat aattacggag cgcagaatta cgacaggatg tttagtactc ttaaaactgc
    14941 cgccctgata gctacttcag ttaccacttt tggtttcctg ctttgtacgc tgtttccgca
    15001 tgcattagta agcattttta ctaccgacga tgaattgaaa cgaattgctg ccaatgccct
    15061 caggatctct gttattatgt ttccgttaat tggttctcag gtcattttta ccagtttttt
    15121 tcagtccatc ggtaaagcca aaatatccat gttgctcagc cttacaagac aagtgctgtt
    15181 tttgatcccg gcagtgttgg tattaccatc cattttcggg ctgaacggtg tatgggcgtc
    15241 acttcccgta gccgatttcc tggcagtaat tgtaacaagt tctacccttt tcttttttat
    15301 ccgaagattt gaaaagaaga acgattacat attcagaata aatcaacctt aggttatttt
    15361 atctaaattg aattcgttaa atactaaaaa ttaagtactt ttgttcactc aaaaagaaag
    15421 aaataaatgt tccggattaa aaagttagat atatatatta tcaagtcatt tttggcttac
    15481 tttttcatga cttttttcat ttgtattctt attctcatga tgcagttcac ctggaaacat
    15541 ctggctgaac tgataggaaa aggggtggaa tggaaagtac tggctgaatt cttttggtac
    15601 actacgctgc aaactgtacc catggcattg ccgttggcca tacttttggc cgcactcatg
    15661 aattttggca atcttggcga aaactttgaa ctgatagcca tgaaatcggc cggaatatcc
    15721 ttgtttcgga ttatgcgagg tctcattgtc tttatagcat ttatttgtgt aggcgctttt
    15781 ttcttttcca ataatgtttt accggttgca caaaccaaat tgtggacact gattttttca
    15841 ctccgacaaa agtcgcccga atttgatatt cccgaaggag aattttattc cggcatcaac
    15901 ggcattaatc tttatgtaag gcacaaagtg cccgaaaaaa ggttgctcaa ggacctgatg
    15961 atttatgatt tcaccaacgg atttgacaat gccaccgtga tgcttgccga ctcaggccgg
    16021 gtgcagttta cagctgataa caagtacctg aaactgaccc tgctcaacgg cgaaagtttt
    16081 gagaacctaa aacagcaaag caataattca tcagccaaca gcatccctta ccggcgcgaa
    16141 acattcagta ccaaagaaat gttgattgat tttgatactg aatttaaccg gtacgacgaa
    16201 tccatattga aagatcagca tatcagtaaa gatattgttc aactgaccca taccatcgat
    16261 tctgtatcca aaattgtgaa aacgagaggc aatcagcaag ccaatgagat gatcaaaacg
    16321 gagtttacaa attttgaaaa caggaatagc gtcagactaa acaagaagga tgctaaaaaa
    16381 ataaatattg attcactttt tctgtctctt tcgcaggaaa aaatggagga agccatgatg
    16441 gtggcgcaac aaaatgtcag ggctaaaaaa gaaaaaattg aattcaacaa agtgatgctc
    16501 gaagaaccca cttactatgt tcgtcggcat caggtggagt ggcatcgtaa gtttacacta
    16561 tcgtttgcct gccttatttt ctttttcatc ggagctccgc tcggtgccat cattcggaag
    16621 ggcggattgg gcttaccggt tgtcatttca gtaatgatgt ttatattcta ttatataatt
    16681 gacacttcgg ggtataaaat ggctcgcgaa ggattttggg aagtgtatca gggtatgtgg
    16741 ctaagctcag cggtgctttt ccccatcggt ttgtttttga cctataaagc ggtaaccgat
    16801 gcatcacttt tccgctccga gcaatacatg aagatattgg ataatgcagt agccggtttt
    16861 ggaaaattgt ggggaaaaaa taacaaaatc caacatacag agcccgaatt atcgacaaac
    16921 taataagggg taaaaaacta tggaaagaaa attcaatacg atagaagaaa ctatcaaaga
    16981 ttttggtaat ggaaagatgg tgatagtggt ggatgatgaa gatcgggaaa atgaaggcga
    17041 ttttatttgt gctgccgaaa aaataactcc cgagaaggta aatttcatgc tcaggcatgg
    17101 tcggggagtg ctttgtgctc cattaataga agatcgatgc gatgagctgg aactgaatat
    17161 gcaggtggaa gtgaatactt ctattcattc tactcccttt accattagtg tcgacaaact
    17221 cgaaggctgt actaccggag tttcggcttc tgaccgagca gccaccttca aagcattagc
    17281 cgatccggca tcaacgcccg aaacgttcgg acgacccgga catatattcc ctctgcgggc
    17341 ccgatcaaaa ggcgttttgc gccgggccgg tcataccgaa gcggctgttg atcttgcccg
    17401 tctggcaggt ctctatccgg caggtgccct cattgaaatc atgaatgaag atggctccat
    17461 ggcacgttta cctcagcttt ttgaacttgc tgaccagttc aaaatgaaga ttattactat
    17521 agccgacctg atagcctatc gtatccgaac cgaatcactc atcgaaaaag gcgacgaagt
    17581 ggatatgcca acagcttacg gacacttcaa attaataccg ttccgacagg tatccaacgg
    17641 gctggaacac gttgtcctca tcaagggtga ctgggaaaag gatgaacccg tgctggttag
    17701 agtacattca tcctgtatga cgggcgatat atttggatcc atgcgttgcg aatgcggtga
    17761 acagttgcac aaagctatgc aattgatcga aaaggaggga aagggagcta tcgtgtatat
    17821 gaaccaggaa ggtcgtggca ttggactgat gaataagatt aaagcctata agctccagga
    17881 agaaggaatg gatacggtag atgccaattt acatctcggg tttcgggccg acgaacgcga
    17941 ctacggcgtg ggagcacaga tacttcgcga aattggtgtg tcaaaaatcc ggttgatgac
    18001 aaataatccc aaaaaaagga ttgggctcga agcattcgga ctctccattg ttgaaaatgt
    18061 gcccattgaa gtgaagccca atgagtataa cgagttttat atgttgacaa aaaaagaccg
    18121 gatggggcat aaactgaaat acgtaggaaa agataacgtg aaatgaacag gacagctatt
    18181 gtaaccggag caacttccgg atttggacgt gcaattgcac ttcgccttgc cgggctgggt
    18241 tatcggttga ttgtaaccgg ccggcgaaag gaattactgg atacactcac cgccgaaatc
    18301 agtgaaaaat attctgtcag cgtattggct ttgaattttg atgtgcgtga ttttaaggca
    18361 tgcgaagaag ccgtcaattc gctgcccgac gaattcaaaa gtattgatct gctcgtcaat
    18421 aatgccgggc tcgctgccgg tgcagcacct tttgaccaaa gtctgctcga tgattttgac
    18481 cggatgatcg ataccaatgt caaaggactt ctgtatatga ctaagctgat cgtacccgga
    18541 atgattgaac gtcaggctgg tcatatagtc aatatctcgt ccattgccgg cattgaagtt
    18601 tatccgtccg gaagtgtcta ctgtgcttcg aaacatgcgg tcaatgccat taccaaaggg
    18661 cttcggattg atctggtgaa gcatgggatt agggtcagtt ctatctctcc cggcatggcc
    18721 gaaaccgaat tctctatagt gagatatcat ggcgataccg aaaaagccaa agcagtgtac
    18781 aacggattga ccccgctgaa tgccgaagac attgccgatg cactggaatt tatagttaca
    18841 aggcctgctc acgtcagcat taatgacatt cagattaatc cctcacaaca ggcaaatacc
    18901 tatatttcgc accgaaaatg aaaatataaa actgtcggaa aattaaacat ctaaagaaat
    18961 tataacaaaa ggattaaatt atttgaaaat ttatcttatt tttgtggttg caaaattttt
    19021 attttgaaat gaattggaca cccatgttga aggtaaaata actttgacat actaaaaaac
    19081 aacagattat ggacgcacct gacactatga atgaaatgga taacctcaat gaaagcctta
    19141 ttacgccgga ggaaatccag gaagtaacaa ccgaaccaat actggaagta aagcccgaag
    19201 ctccagtcgc acatgacctg accaaacagg aaatctgtga ccggatgaaa gaacttatcg
    19261 aacagaatgt ggagcaggtc aaagaggaag ttgagtccat gaaacaatta ttctataaaa
    19321 agagtaaatc agaagttggt gaacaacggg ctgctttcat cgaaaaaggt ggagttgaaa
    19381 ctgaatttat tcccgaaccg accgaacttg aagctgaatt caaagaactc ttaaatgaat
    19441 tcagaaacaa taaagcaaaa ctgaatgctc aacaggaaca ggagaaaggc aataatctgc
    19501 tgaaaaaaca gcacattatt gagcaaatga aacagctggt cgagagtaac gatgatgtgt
    19561 cggccaatgt agctgcattt cgtcaattgc agcaagactg gaaaaacacc ggtcctgtac
    19621 ctcctacagc aaccaacgaa ctttggaaac aatacaacct gtatcaggag tctttctggg
    19681 atctgatcaa gataaacaac gaactcaggg aatacgattt caagaaaaat ctggaagcaa
    19741 aaactaaact gtgcgaagct gccgaaatgt tggctaacga agaagatgtg gtttcggctt
    19801 tccgtcagtt gcaaaaactg catgaaaact ggcacgaact tggtccggta gcacgcgagc
    19861 ttcgcgaaga aatctggaac cgattcaaaa cagcatccac tacaataaat aaaaaacatc
    19921 agtcgcactt tgatgatatc cgcaaggtgg aagacgagaa tatggcgttg aaggcatcgc
    19981 tgtgcgaaag aattgaagcc atcgatacca ccaaattcag ttcgtacaag gattgggacg
    20041 aagccacaaa aattgtactt gaagcacagg aagaatggcg ctctgtgggt tttgcaccac
    20101 gcaaggctaa tcagaaaatt ttcgatcgtt accgcaaagc ctgcgataag tttttctcca
    20161 ccaaagctga attttataaa tccataaagt caaatctgac acagaatctt gaacagaaaa
    20221 aactactctg tgaaaaggca gaagccatta aggatagtac tgactggaaa gaagcgggtg
    20281 atcaactggt acagttacag aaagaatgga aaaccatagg accggtagcc aagaaatatt
    20341 ccgatgatat ctggaaacga tttattactg cctgtgatta tttcttcgaa cagaaaaaca
    20401 aaaatgcatc cggtcagcgc agtgtagaaa tgcaaaatct tgacaagaaa aaagagctga
    20461 tagcaaaaat taccggtttt gaaaaaacag gtaacgccgc tgaatcgctg gctgccctgc
    20521 gtgcactggc tgccgagtgg aatgctttag gtcatgttcc tttcaaggaa aaagataaaa
    20581 tatacaaaga atatcgtgcc gcactcgatg ctcagtttga aaagctgaat attgatgttt
    20641 cgcagcgtag gttggagaca ttccgcaata acctgaagga tatgacctcc aaaggagaca
    20701 acaaactgta tcgggagcgt gaaaaacttg tcagagctta cgaacatctc aaatctgaaa
    20761 ttgccaccta cgaaaataat atcggtttcc tcagcattgc ctccaaaaaa ggcgacggaa
    20821 tgctcaaaga gatggagcga aaaatcgaaa cattgaaaga ggaatgcaaa ctgctggagc
    20881 agaaaatatc actcattgac gatcaaatgt aaatatctta tattattatc caacagaaaa
    20941 gcaccgggaa attccggtgc ttttctgttt taataggatg aactccatca ttggtggaga
    21001 gttttattca atatttcttt tgtggttttc cctttttggt tcaattacca gtggatttgc
    21061 attcatagca atgtattcca gtctgttttt gtaaatatcg caagccagat acagtgcttc
    21121 cagaaaagat tgctccgatg ctttgttttg gccggccagc gtgtaggcag taccgtgagc
    21181 gggcgatgtg cgtataaccg ataaaccggc cgtatagttt acaccgcttt ccatggacag
    21241 ggtcttaaaa gctatcagac cctggtcatg atacatggcc agtatggcat cgaaattacg
    21301 gaaattacca gatccaaaaa aaccatcggc agggtaaggc ccaaaacaaa gtataccctg
    21361 actgtcagcc tttttaaggg ctggaattat tacattttgc tcttcattac cgattacacc
    21421 atcatcacct gcatggggat tcaaacccaa aacagcaatc cggggtttga caattgaaaa
    21481 atcttttttc agcgaactat tcagtatctt cagtttctcc actatcaggt cttccgtaat
    21541 aaccgatgca acattgctga cgggtatatg gccggtcaca acagccaccc gcatgaaatc
    21601 atttacaagc atcatcagag ccgatgattg ttcaccaaat tttgtttcca gatattcggt
    21661 gtgaccggga aaatgaaaat cagcggattg aatatttttt ttatttatgg gagccgtaac
    21721 gattgcatgt agtcttcctt tttttaaatc ttcagtcgct ttttccaatg cttcgtaggc
    21781 cgctttaccg gcttcagcag tagactgtcc caactcgact ttcaattctt cgtccacgca
    21841 attgatgata ttgatccgtt tcgaatttgc ttcttcgagt gaattgatca cgttcagatt
    21901 tagtccctgc aaatcaagat tttttttgta aaaacctgcc agtttagacg agccgtatat
    21961 caccggtaca aacatatcca gtacccgatt gtccgacaag gcttttagaa taacttcgta
    22021 gcctatgcca ttgatatcac cgtgtgttat tccgattatt attttttctg gttccatatt
    22081 atttatttta tttgtatgaa atgatgtttc tgtttgtatc cgaaaagaaa ttcagcacat
    22141 atttcatcag tttattgcgg tcatgatcgt ctctgatggt ttcgaaggga aatccaagcg
    22201 cacaaatccg gtagtttcca tcgtatacga tgcctgctcc ctgattgttc tcttcaaatt
    22261 tgcaaatctg ataagcgaat ttaccgttcg gggtaatgat atccggcgct tccacatagt
    22321 acgattcttc attcggggaa gcatgaaatg agacatttaa atccttaaaa tgattaatct
    22381 cgttgttatt aaaattgacc ttatgaaggt cgtttatttt atccgacatc catttgattt
    22441 tcagcgtgtt ttccacaaag gagcgatccc gatcggtgct gttttttgat aaaaccaaat
    22501 ctgaaataat atttgaaccg cttaccagca gattaccacc cgaatcggtg tattgcatga
    22561 tcgcttgttg catttcaggg gtaaaagttc tgaaaacggg ttgtgtggcc tctttgttca
    22621 aacttgtcca ctcgcgctct ttgcccagta tcaggtccac gaaagggtag caatacagct
    22681 ttatgtcacc gtcggttacc gatttttcac tgcatgaaac aaatgaaaat ccggcttttc
    22741 ggatactttc tccgtgtagg tatggataat cgaaggtatt tccagcaatt acctttcctt
    22801 cccaaccctc ttcactggct ccgtgaccgg gattttcgtc acttcggtag gggcggtttt
    22861 tgcgaaattc ggtctgaact cccgtaaact tataatcgtt gatatagggt actccggcat
    22921 cccagctatt gttaaagccc gaaacatcac tgctcacaaa gctggcagga gccgaaatgc
    22981 ggttaaatgc attgacaata agtactacat tggcatcatc ttttgtttta cataccgaaa
    23041 ggatttcgga aggaaaactt ctgcctcctt cattcacagc ctctactttg aaactataaa
    23101 ttttatttct ttcaattttt ttgtaaaatc gggttgaatt cacatattct ccgttgtcga
    23161 atccaccatc ctccatgcgt gtataaacaa gataaccggt aggttttgca gtaggttcaa
    23221 gcgggtcgtc caccggtttc cagcgcagct ctacttcggt ggagtcggtg aaatcggcac
    23281 agaaagcctc taccggcagt ggttgaactt tatattcata attattggta ctggccagat
    23341 aacgcaatat gcccttgtac atggcacggc ttgccgtaaa acggaaacgt ggatcgagcc
    23401 cgtattgcat gtcggggaaa ttctggtgcg aaaggagttc aagtagtacg gaaggcactt
    23461 cgggtacgcg tgattcactg taattacgat tccagagttg tcgtcgtacc cagtccttat
    23521 catacaattg ctgaacatcc cgaacaacct gcgtttgcac actgtcggtg aggtcgcgtg
    23581 ccacccagcg tgatatgccg ttgttgtatt ttacagctcc ggtctttgtt ttcactgtat
    23641 gaattgccag tgtgccaatg atggagtcgg aggtttttcc tgcatcggta tgaagcgcaa
    23701 aagccatatc cagtggaata ttcaatcctt tatacccggg cgccgataca gagccgcccg
    23761 cgagataatt cacccaaaat ccacgtgact gaaaatcgtc ggagtagtca ttttcccctt
    23821 ttgtgcggct atatatagaa tccggtacac ctgcccactg aagccagtac cgcgctcctt
    23881 cggtaaaccg cggcattccg ctggtactgt atgtgtcgaa atgctttact gtcgttatgg
    23941 ttttggtctt gtatttaatc cgtcttttct tgcctttctt tttcttgctt ttaatcggga
    24001 ttttaataat ttttttagta accaaatcgg agcgtttccg gctgatattg ccaagtcctc
    24061 cacccacttt tacggcatca gctgtaatta gtttgtgaat gtcagtgctt gtgtttgtca
    24121 gtactatttt tgactgttga ggctggtttt tgtgaaatgt gaaatgaccc agatagatcc
    24181 aggttccgcc acccatagtc tgattaacag catagcgggt ttcgccaccg gcatgatata
    24241 ccgtgtaaat ggcttcattg ctgctgtttg gtaccgtttt gtaggagata tatactgcgt
    24301 acgagcctga ttttggaact gaaaaatgcc attctgcggt acttgaaggt tcgtcgtgac
    24361 caacagtatt tgcctgaatg aatgtgccgg ttttgaatgg gttttcaccg tcgcgatagt
    24421 tttccttgaa gtgggcaaaa ccacttccca cacctttgtg ccaggtaaaa tttcccgtag
    24481 tggttctgaa gctgcaatcg ggcgaactct tatcattgtc cacaatcagt tctatccgct
    24541 gtatgtcccg ttcacggggc agaagtactg tggcacctgc gttttcgagc attggaacca
    24601 aaagtggcag aatgtatgat tgtgtgtaaa gatcttcccg tgtttcaaac aaattggccc
    24661 gctgccagtt ccatttttct ttggattgtt tataatacaa cccatggctt tgccagagag
    24721 caatatgtct gttgtccaat cctttagtag aagaaaaagg tgatgatata tttttagtta
    24781 acggacggtc tgttcgggga attacaaatt tttgtttaat aggcagagta ggaggtttct
    24841 ccgtggggat gaccgtcgta tccacttctt ccgtatattt ttctttggta gtaatggtgt
    24901 cggtcacaac agtagataat cctgtcgaat cgttttcttc ggtaatctct ataattgtgt
    24961 cattcacaaa atttgatccc ggagatttat cgacttttat ggagtgtgca ggtgcaaaac
    25021 cataaaagag caacatacag cttgtaaata gtatcgtgtt tttaatgaat ttcatcttcc
    25081 aatctcattc taagaaaatt atcaatgaga accatcatgg ttttgcaatt tctctttggt
    25141 tgacagcatg ccgcaggctg cgtgaacatc ttcaccgcgt gaacggcgga tggtggtgat
    25201 tatgttgttg ttatttagat aattgcgaaa aaaatccatt ttttcagggc tggaactttt
    25261 cagctcaatg cccggtatct catgaaatcg tatcagattt agccggcagt ccagtccttt
    25321 cagcattttt gccagttcta cggcatgccg gatgctgtca ttcagtccgt cgaacatggt
    25381 atattcgaac gataatcttc gttgatgcct gaaatcatgc tttcggagtt cggcaattac
    25441 tttctcaatg ggataaacct tttccattgg gatcagtttc tccctttcct cactgaaggg
    25501 ggaatgcagg ctgatggcca ggtgacaatc cgacttttca agaaaaactt tcatgcccgg
    25561 aagaatgccg atggttgaaa cagttattct gcgtggactc cacgcaaaac cccagttagc
    25621 ggtaattatt tcgagcgatt tcagcagggt cagcaaattg tccatcggtt cgcccatgcc
    25681 cataaatacg atattggtca gcttgtcgga atcgggcaca gaaaggatct gattaatgac
    25741 atcagccgga ctgagatgag cgctgaagcc ctgtttgccg gtcatacaga acgaacagtt
    25801 cattttacaa ccaacctgta tcgaaacaca aagggtggcg cggtcttcgt ctggaatata
    25861 caccgtttcg atgtaatgtc ccatttcagt tttgaagaga aattttttag tcccgtcatc
    25921 cgatacttct gtctgtatag gttcatagct tccaacttcg tatttttcgc tcaggatttc
    25981 ccggttcttg accgatatat tactcatcag gtctatcgag ccaattttct ttttataaat
    26041 ccattcggca atttgtttgg cggtaaatga cggcatggac aattctgcag caatctcttt
    26101 caattcgtcg atcgatttac ctataagtgc ttgttttctc attgtttatt gtatgaactt
    26161 tcacaaaatt acgataattc gacggaaaat aaaaacggaa tgaagtatat ccattccgtt
    26221 ttataatgtc ttaggaaaat tatacgaatt tcctcttaat agaaattaga gcgattgtca
    26281 actatcttat atttttcttt cagtttttcc atcagggtgt atggcaatga atatgccatg
    26341 cgcgagtcaa gttgtgcgga ttccattttg gcattgaacg gggcgttgtc ggccactctt
    26401 gcaccgggca gaataacgta aacaccggcg tttcctttga gtggagcaga tactttgccg
    26461 gtagggagta ctgaagcttt gccaatcaca tagggttcga aaccggcttc gcccagttga
    26521 taaccggcaa aattaactgc cggggctgtt tttacctctg tattgagtgc cggagcaagt
    26581 gtttccagtg ttgggtattt ggccagtaac gtgcttaggt ttttaatcat caagtcagct
    26641 tttttgtctt taatgatttc agctttcagt tgagcagatg cctgtgctac tgttttataa
    26701 ccttcttcgt taatttcggt tgtggtggct actataaatt gtgttccgca atcgaacaca
    26761 tcggatacgg tacctttttt attttcaaaa gcccagcgaa ctacctggcg tgattgtggc
    26821 acggcattaa cccggtcaga gtttttgagc aatccgatgg ccggattaat cacatatcct
    26881 ttttgttttg cattggcttc gaatttttcg agggaggtac tgttggcggc aaattctttg
    26941 gcaccattgt aaattttgct gtacgactga ctgcttgggg tcactttgcg ttcaagaata
    27001 gccagtttca ctttgcggcg tgcaggtgtt ttttcggttg cctgcatgat ctggataccc
    27061 tgagctgagg taaccttgaa gatttcattg gtcggttttg aaaaagccgg accggtaatt
    27121 tcttttccca cagagccttc tgtaatccag cctatttcac caccattggc agccgtttct
    27181 tttaccgctg agtatttcat ggccatatcg gcaaatgtgg atcctgattt gagggcaccg
    27241 ataatgctgt cagccttttt ttcgtcgtta ccggccagga atatgtggcg cagcttcacc
    27301 gagtcggact gcatgatacc ggattccatt acgcgtgcca tggtgtaggt gttattttgg
    27361 aatactggac catatatagc acccgtagca tttccgaaag caaagtcttt caggctggcc
    27421 ggaactgatt tctcggagta aaggcgacca tcgtaactga tgtccgattc cgaattaact
    27481 actgcggcaa catctgtagt ggttttgaat tcttcgctaa cttttttgat ccattcctct
    27541 gcttctttga aatcttcctg aagtggtaca acgtcaaagg cgacataatt gatggagcgg
    27601 ttggcttcct gtttgaaatt ttctttttcc ttgttgtatc tttctttaat ttcggcatca
    27661 gtaaccttta tggcagaatc tgaaacagca aaatagggct gcattacata gttgacgtca
    27721 accgaagttt tgcgggcatc atagttgtat tttgcttcta tggtgtttga acctacagat
    27781 ttcgaaatca gagcggcata tttttcctga agaatggcat tttttacggc tttttcccag
    27841 aataaccaat agttttttgc ctcttttaat tgttcggcat ctccattgga agctccaaaa
    27901 atattattat agaatttcag cagattagcc gggctgaact gtcctttttc gtccatgaaa
    27961 accctgcgct gcatgattag cggatgaata ttttttccta tcaggcggtc ggtaagttcg
    28021 tccttactta cacttaaacc cagttttttg gcttccgaag caagaattct ctcgtttacc
    28081 atattgtccc ataccgaagt acgcagttgg gcgttttgtt cttcggtgaa atcgctttgt
    28141 ccggtttcta ttttatacac attggtcatc tgctcaatag cggccgaaaa ctcactgata
    28201 ttaatttttt ctccggcaat ttcagccacg ttttgtcggg cctgatgaaa aagtgtagaa
    28261 cctgaattta aaaagtcacc aatgataaat gctaataatg cagtacctac aatgactgca
    28321 agtaatactc ctttgcttct gatcttgtct aatgttgcca ttttattaat aaattgataa
    28381 atatttttta tagtttttaa ttcttaaatt ttgagggcac gaatatacaa aaaatttggg
    28441 tattcccgtg ctttttttag taattattta cgacagacat tgatttccaa cgggataagc
    28501 tctgttactg ttagttgttt acgagcagtt taaccatttc aatccggttg ttagttactt
    28561 taacacactt gaaggtgaaa ttttctatcc gaataatttc attgagtttg ggaaagtgct
    28621 gatgtatgtg caaaatatat cctgcaatgg ttgtataatc atcagatacc ggtatgttca
    28681 ggttgaaaag tttgttgact tcttccactt cgagccttcc ggcaaaaaga tattcgttct
    28741 cccctgtttt ttcggctgta tatccccgta tgtcgtgttc gtcttcaata tctccgaaaa
    28801 tttcttccat aatatcttcg agggtgacaa ttcctgcagt tccgccaaat tcatccacca
    28861 ccacagctat gcttttcttt tcgctcataa atagcttcat cagtttttga gcagccatat
    28921 tttcaggcac tatgggtatc tggcggatgt attttgtcca gtccttgcgg tgctcaaaca
    28981 tttcggacga gtgaatgtaa ccggtaatat tgtcaatatc gcccttataa atgattattt
    29041 tcgataatcc ggtttcgata aatgtttgtt tcaaaatgtt gacgtccaca ccatattcca
    29101 gagccacaat ttcggttcgg ggcacaaagc agtcacgcaa tttcacggtc gaaaaatcca
    29161 gggctttctg aaaaattttc acttcatgtt ccatctcttt ctggtcgttt tttgtgtcaa
    29221 gattttcctg tagcaggtag ttgaggtcaa tgcgtgagaa aacattttct tcggtcgagg
    29281 ccggcaaact gattttgaaa agactgaaaa acttgctgga tgcccacgag ctgaaccagg
    29341 aaatgggata taaaactatg tagaaaaaca gcaaaaccca cgagaaaagt ctcaaccaca
    29401 tgttgggatt gattttgaaa aatgccctgg gcagaaattc gccaacaata ataatcacta
    29461 ctgtggcaat ggttatttgc aataaaatgc tgataaaagg gcttaaagct ccctcgaatg
    29521 cgaatgtgag cgactctgtt atgagcaaac caaaaactac catgctcaga aacttaccca
    29581 caagcatggt ggaaataaat tgctgagaat ttttgaaaaa tatcgaaata atggaggaag
    29641 taagattatt gtatttctta tctaattcga atctcaactt attggatgaa ataaaagcga
    29701 cctccattcc ggagaagaag gccaaacaaa ttagcgatat gataatgtta ctgacgtaga
    29761 tcaacggtat gcaatttttt gtacttaaat ttttagaaat caaagtctgc caaagtcatt
    29821 atttcggatc aatttctttg caaagataca atattttaag cttttaccat acgttcggct
    29881 gaaaaatata ctgaagatta aaccgggaaa aaatagtgga aaaatt
//
LOCUS       DAOPZK010000006        29628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1548_length_29628_cov_22.164339, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000006 DAOPZK010000000
VERSION     DAOPZK010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29628
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1548_length_29628_cov_22.164339"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..81)
                     /locus_tag="PKH68_00720"
     tRNA            complement(9..81)
                     /locus_tag="PKH68_00720"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Thr,seq:cgt)
     gene            complement(223..915)
                     /locus_tag="PKH68_00725"
     CDS             complement(223..915)
                     /locus_tag="PKH68_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11487.1"
                     /translation="MKTYKIILFILLLFPTFNLLAQWETTPTIYSEHFFTGYYENNLH
                     ARNVIDYKDIKGSPYQNPKFTPGKLYMKDTVVVSAPLRYNISTDEIEYEADGKAYAIG
                     NPGFLNKVVIGNSTYVYLPFIGKGGFYELLETGECHLLQRRTVRFKPVEYAKPIIGTV
                     PAEFIPEPDSWYLVVDGNQAYKIVNLKSVRHALADKEPYMEHFFRQQKIKSIRKENLI
                     KVVKYYNSLKDQ"
     gene            1207..2268
                     /gene="carA"
                     /locus_tag="PKH68_00730"
                     /pseudo
     CDS             1207..2268
                     /gene="carA"
                     /locus_tag="PKH68_00730"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
     gene            2368..5574
                     /gene="carB"
                     /locus_tag="PKH68_00735"
     CDS             2368..5574
                     /gene="carB"
                     /locus_tag="PKH68_00735"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HNX11488.1"
                     /translation="MKKVLVLGSGALKIGEAGEFDYSGSQALKALREEGVETVLINPN
                     IATVQTSEGIADKVYFLPVTPDFVEQVIEKERPDGIFLSFGGQTALNCGVALYRTKIL
                     EKYNVKVLGTPVQSIIDTEDREIFNQKLSEIDVKYIKSEAVTSLDDALRAANTLGYPV
                     IVRAAYALGGLGSGFCDNDDELKTLVNKAFSYSPQVLVEKSLKGWKEIEYEVVRDRYD
                     NCITVCNMENFDPLGIHTGESIVVAPSQTLTNREYYKLRELSIRIIRHIGIVGECNVQ
                     YAFDPVSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLAELKNSITKVT
                     SAFFEPALDYVVCKIPRWDLGKFHGVSRELGSSMKSVGEIMSIGRNFEEVLQKGLRMV
                     GLGMHGFVGNKEFETGNVEKDLAVPTDRRIFFLAQAFHEGYTVDRIHELTKIDKWFLY
                     KLQQIVDMERELSGFDKLSDLSDELLLEAKKKGFSDFQITRMVVKSNSHDMDDETKKT
                     REYRKSRGIFPVVKQIDTLAAEYPALTNYLYVTYNGSTTDVHYMGDHRSVVVLGSGAY
                     RIGSSVEFDWCGVNALTTIRNQGLRSVMINYNPETVSTDYDMCDRLYFDELTYERVMD
                     ILEAENPMGTIVSTGGQIPNNLAMKLAAANMRILGTQAEDIDRAEDRNKFSAMLDELK
                     IDQPRWQELTTYSDVTRFVKDIGFPVLVRPSYVLSGAAMNVCYDETQLKTFLDLATEV
                     SKKHPVVISEFLERCKEIEIDAVAEKGEIVVYAISEHVEYAGVHSGDATTQFPPQKIY
                     VETIRRIKNIAREIASSLNISGPFNIQFLARDNYIKVIECNLRSSRSFPFVSKVLKIN
                     FIDLATKVMLGISVEKPEKSAFDLDYVGIKASQFSFTRLQHADPVLSVDMTSTGEVGC
                     IGDSFSEAILKSLLSVGYRIPQKRIMISSGTTKSKVDLLDACKLLDEKGYELYATLGT
                     QKFLKDNGVKAIAVNWPDEEGDYNIKEMIANKKFDLIINIPKDLSRRELTNGYIIRRS
                     AIDFNIPLVTNARLASAFIIAFCTLKKEDIKIKSWQEY"
     gene            complement(5664..6125)
                     /locus_tag="PKH68_00740"
     CDS             complement(5664..6125)
                     /locus_tag="PKH68_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11489.1"
                     /translation="MELKRNLADKNNKSLIHTAIGIVFIAVGLYYMIDKINHKTFDQF
                     DWAYLAFYAVFGFKYLIQGVIALIRRPYIVINNDKISLKPDASTPGKTIWWRDIQSIT
                     QVGKNYKIRSSDNSSYTIHLSYYNYEVADEIKVALETIASEKAIQINEQQN"
     gene            complement(6162..6764)
                     /locus_tag="PKH68_00745"
     CDS             complement(6162..6764)
                     /locus_tag="PKH68_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11490.1"
                     /translation="MKKNVFLLTATFIFTLNTFSQQTLDEYKMIKVPHQLSLETGYRN
                     VFSLIDRSGVMNGNSATHGVGALFDYGWQVSGLDGKKPAVYLTVPIGYTKLFADYATS
                     KDISMLNYGWTVRHNLNRDKRLTPFLGYGLLLNTLKIEDIAGGVMGHQTQFELGTNLN
                     TDSRLQYFAKIQYSYTSYPSLNNAKRIHFQFIDLRIGARF"
     gene            complement(6958..7482)
                     /locus_tag="PKH68_00750"
     CDS             complement(6958..7482)
                     /locus_tag="PKH68_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX11491.1"
                     /translation="MKFLENDKIVLRAVEPEDLELLYRWENDSSIWIAGNTRAPYSRY
                     QLKNYISHSPTDIYESKQIRLMIVSKIENKTVGTVDLFDFDIHNSRIALGLYVAEEYS
                     GRGMATESLKTVEEYVFNFLKINQLYVQIIESNLVSRKLFENDYELHGKLKNWIKTPA
                     NYEDVLTYQKFRKI"
     gene            complement(7517..8476)
                     /locus_tag="PKH68_00755"
     CDS             complement(7517..8476)
                     /locus_tag="PKH68_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX11492.1"
                     /translation="MVWKDYFYFSKSQRRGIIVLIALILAALLSTFLMPYFIKENDKT
                     ENTVFLKDAEKFKASLTEKERSRQNQWDNYPDFHKSYTKKSSETSHYQLFAFDPNTAD
                     SATFVRLGLKPYIAKNILKYRSKGGKFRKAEDFSKVYGISSEKAGELMPYIQIAEMKP
                     TERKATGQESQAETTEKPAMQFTESKTTIVELNSADTTALIAVKGIGRRFAKSILGYR
                     RILGGYHSVEQIREVYGMKPEIFEQIKPFLSTDPSHISKIYINKASVERLKSHPYLTF
                     SKAKEIYELRRDRGKLKNIDELRKLHSLSDEDLLKIEPYLSFE"
     gene            complement(8560..10041)
                     /gene="proS"
                     /locus_tag="PKH68_00760"
     CDS             complement(8560..10041)
                     /gene="proS"
                     /locus_tag="PKH68_00760"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNX11493.1"
                     /translation="MAKELKELTSRAVDYSQWYNDLVIKADLAESSAVRGCMVIKPYG
                     YAIWEKIQAELDRMFKETGHQNAYFPLFIPKSFLSREAEHVEGFAKECAVVTHHRLKQ
                     DPNGTGLIVDPEAKLEEELIVRPTSETIIWNTYKGWIQSYRDLPILVNQWANVVRWEM
                     RTRLFLRTTEFLWQEGHTAHATRDEAIEEAEKMLHVYADFAENFMAVPVLRGVKSPNE
                     RFAGAIETYCIEAMMQDGKALQAGTSHFLGQNFAKAFDVTFVNKENQLDYVWATSWGV
                     STRLMGALIMTHSDDNGLVLPPNLAPFQVVIVPIYKNEEQLNAISEKVKEITSKLKAL
                     GITVKYDDNDTKKPGWKFAEYELKGVPVRLAMGARDLENGTVEIARRDTLTKETVAME
                     GVEQYISDLLKEIQHNIFKKAVDFRTSVTREVNSYDEFKVEIEKGGFLLCHWDGTAET
                     EEFIKNETKATIRCIPVDGEVDPGFCMVTGKPSTRRVVFARAY"
     gene            10331..11569
                     /locus_tag="PKH68_00765"
     CDS             10331..11569
                     /locus_tag="PKH68_00765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX11494.1"
                     /translation="MRFDELPLCDAVLDGLQAMNFTEMTPVQEQAIPVILQKRDLIAC
                     AQTGTGKTAAYILPLLNNLQTTPHTDDAVNAIIMSPTRELAQQIDQQMEGFSYFVPTS
                     SVAVYGGNDSRAWDVQRNGLQRGADVVIATPGRLISHLNLSEIDLSHVRYFILDEADR
                     MLDMGFFDDIMQIVNRLPKERQTIMFSATMPAKIRQLAKTILRDPVEVKIAISRPPDS
                     IMQTAYICEEGQKPAIIRNLFSEKSPEKVILFSGSKLKVKEITKTLRRMGLSVGEMHS
                     DLDQAQRDSIMHEFRNNRVNMLIATDIVARGIDIDDITLVINYDVPWEVEDYVHRIGR
                     TARAGENGMSITLVAPEEQYRFKQIEDFLEKSIFKIPLPEGIGEAPEYNPEANRRRKK
                     QFSAPKGNFKRKHHGSGKRN"
     gene            11623..12564
                     /locus_tag="PKH68_00770"
     CDS             11623..12564
                     /locus_tag="PKH68_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNX11495.1"
                     /translation="MSKYPDFLKEGDEVRIISPSGVIDPTLVDGAIILLASWGYKATE
                     GMFTRNALGRFAGNEQERFTDLQQALDDPNVKAILCSRGGYGLAQIIDRIDFSRFVKH
                     PKWVIGFSDVTILHQALLSTGIISLHSAMAKQFTELPAKAEPLVMIENILRGDIPSYK
                     ITTNKHNRSGIGKGKLIGGNLSVFMGMRGTGYDLPFKNSILFIEDIGEKPYQIDRMMQ
                     NLRISGALSQLSGLLIGQFTEYEEDPEMNKTLFQIIADAVKEYNYPVCYNFSAGHVDN
                     NFPLLMGANAELKVTDRGASLSFQTTFWKKAGNLLNL"
     gene            12627..13232
                     /locus_tag="PKH68_00775"
     CDS             12627..13232
                     /locus_tag="PKH68_00775"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNX11496.1"
                     /translation="MFSGIVEESATVVALVPEQENLHLTLNCSFVNELKIDQSVSHNG
                     VCLTVVSKTADTYTVTAIKETLECSNLGKLKVGDKVNVERSMVMNGRLDGHIVQGHVD
                     QTARCTRIDEADGSWYFTFEYEFDQAMARKGYMTVDKGSVTVNGVSLTVCNPTNNSFQ
                     VAIIPYTFDHTNFGQIAVGTYVNIEFDIIGKYISRMLEFNK"
     gene            13356..14540
                     /locus_tag="PKH68_00780"
     CDS             13356..14540
                     /locus_tag="PKH68_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11497.1"
                     /translation="MDELKKQIKDSFFNPILYFLPLLIFFVVDDMFGLNMAWKISFPV
                     ALGLVFYVYLSYNRLFLWHLMLSVGYVLIGLIVSLIPARYEMRGLYQFADEFLFLGMM
                     CFFIFRKSFLEKVASKALPYEMPMSNNVNELYRIVKYLMVIVSTYMLVRVLYFFGIIN
                     MTENQLQDIQMVYYYCFIVLGLYETIRVLIVRYRLLHEDWLPIIDDKGKVTGSSQYFH
                     DLTERKHMHPVVRLHFIDNGVIFLRQRKSHDNTDASLWDTPVSRHVRLGESVEQSLRY
                     KSKQYYNIEPKKFFFLTNYKLENKSEYQFVYLFVLCKIDGIELNKDKVQATKWWTVNQ
                     IEDNLNTGIFTERFIKEFELLKRSGLLEHESCNCECALKGMIKSRLIDGNTGRGAQNS
                     AC"
     gene            complement(14537..14914)
                     /locus_tag="PKH68_00785"
     CDS             complement(14537..14914)
                     /locus_tag="PKH68_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNX11498.1"
                     /translation="MKKILLVILGSICLGLAILGVFLPVLPTTPFLLLSAALYARSSQ
                     KLYDWLLNHKIFGEYIRSFREDKSIPLNIKILSISTMWLVMLYSIFFVVNEKWYLQVM
                     LAGIALGVSIYILSFPTKIKVKP"
     gene            15033..16706
                     /gene="ettA"
                     /locus_tag="PKH68_00790"
     CDS             15033..16706
                     /gene="ettA"
                     /locus_tag="PKH68_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNX11499.1"
                     /translation="MADDKKVIFSMVGVSKLYPPQKKVLNDIYLSFFYGAKIGIIGLN
                     GSGKSTLMKIIAGVEKSFQGEVVFSPGYSIGYLEQEPVLDPNKTVKEIVQEGTQEVVD
                     MLAEFDQINEKFADPDADFDKLIIRQGELQELLDHADAWNLDNKLDRAMDALRCPPED
                     ALVKNLSGGERRRVALCRLLLRQPDILLLDEPTNHLDAESVEWLEHYLHNYAGTVIAI
                     THDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKTTRMAMEEKQVSKRRKTLEREL
                     EWVRMAPKARQAKGKARLEAYDRLLNEDEKEREDKLELFIPNGPRLGNKVVEAIDVSK
                     AFGDKLLFENLNFMLPPNGIVGIIGPNGAGKTTLFRLMMGLEKVDKGTFETGETVKIG
                     YVDQSHTDIDPQKSVYQVISGGTEFIRVAGKDINARAYLSRFNFTGGDQEKLCGVLSG
                     GERNRLHLALTLKSEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVVISHDRWFLD
                     RICTHIIAFEGDSQVFVFQGGYTDYEENKKQRLGDSGEPKRIRYKKLIV"
     gene            complement(16749..17426)
                     /locus_tag="PKH68_00795"
     CDS             complement(16749..17426)
                     /locus_tag="PKH68_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX11500.1"
                     /translation="MNRIFSKKFLLVIFSVFFFIGGSNAQGNLPYVDDKPLHFGYALG
                     LNFMDFAVVPADINKDVSVTAVAPGFSVGAISDLRLNRYFNLRFTPTLMLSQRTLNYK
                     NYDPAQILSVPLYLPLYIKYSSERKDNYRPYVLAGGGLWMDWGRNKERAVLLNPFDAL
                     VEAGVGCDLYFPFFKLSPVLKFAVGVSNVITPTAMRDTAPASGPGPTDAISKLTTRMI
                     TLAFNIE"
     gene            complement(17556..20519)
                     /gene="infB"
                     /locus_tag="PKH68_00800"
     CDS             complement(17556..20519)
                     /gene="infB"
                     /locus_tag="PKH68_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNX11501.1"
                     /translation="MSIRLSKACKDLNVGMATAVEFLAKKGHKIAADPNSKISDEEHL
                     LLAKEFNKDMAMKLESERLSQERQAKEKAETIALEGFQKKPEKKEEMIPTTVSSDLRP
                     HIKEVGHIDLDKPKPTPPATPKVQKPEPVAEPKAAPVVAQPDIKPASDALPKEEKPVE
                     IPEVKVVQTETITTTPPESSESKLKEEVITEPATEKRIIEIKKKEEEKPVAPKPVIET
                     TNVEEEEEEDEKEEEIYRIGKRGITPTPVVIGSIDLDSLNQSTRPKSKTKEQKKKERD
                     ERLKNYVDGKPKKGEPVKDVKIEPKAEVKTDAKGAKRKRINTQRVDINKPEPLDFSKK
                     QATKKEHFKKPIKTVVNEEDVQKQIKETLARLSKANNKRGKGSKYRRDKRDIVSAKLQ
                     EQFDRDKSEENVLKLTEFVTVSELATMMNISVNEVIRTCMSIGMMVSINQRLDAETIN
                     IVADEFGFKTEYVSQDVIEALAESEGADSEEDLEFRSPIVTVMGHVDHGKTSLLDYIR
                     KTNVIAGEAGGITQHIGAYNVKLDNGQRITFLDTPGHEAFTAMRARGAKITDIAIIIV
                     AADDDVMPQTREAINHAVAANVPIVFAINKIDKPGANPEKIKETLANMNYLVEEWGGK
                     YQSQDISAKKGLGVKELLEKVLLEAEMLDLKANPNRRAIGSIIESSLDKGRGYVATVL
                     VQNGTLRQGDIVLAGINHGKIKAMFNERNQRLNEAKPSEPVLILGLNGAPQAGDTFNV
                     VESEQEAREIANKREQLQREQSIRTKKHFTLDELGRRIALGDFKELNIIVKGDVDGSI
                     EALSDSLLKLSTPHIQVNVIHKAVGAISDSDILLAAASNAIIVGFQVRPSVSARKLAE
                     KEEIDIRLYSIIYDAIEEIKSAMEGMLSPEIKEVVTATIEVLEVFKITKVGTVAGCIV
                     REGKVKRNNKVHLIRDGIVVFTGDLDSLKRFKDDVKEVGVNFECGLNLKNFNDIQVGD
                     MIESYEEIEVKQTLK"
     gene            complement(20600..21853)
                     /gene="nusA"
                     /locus_tag="PKH68_00805"
     CDS             complement(20600..21853)
                     /gene="nusA"
                     /locus_tag="PKH68_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNX11502.1"
                     /translation="MSKKENINLIDTFSEFKELKNIDRSTLISVMEESFRNVISKTYG
                     TDENFDVIINPDKGDFEIYRNRTVVADDELTNENMEIRLSDAQKIDEDYQLGEEVTDT
                     VDFFGFGRRAILTLRQTLASKILELQKDSLYVKYSEKVGEIVSAELYQIWKKEMLLMD
                     EDGNELYLPKAEQIPTDFYRKGETVRAVVAMVENKNNNPKIILSRTSPVFLQRLFELE
                     VPEIHDGLITIKKIARIPGERAKVAVESYDERIDPVGACVGVKGSRIHGIVRELRNEN
                     IDVINYTNNLSLFIARSLSPAKITSIRLNEADKKAEIYLKPEEVSQAIGKNGLNIKLA
                     SMLTGYTLDVFRDIDETQNEEDIYLDEFSDEIDQWIIDALKAIGCDTAKSVLSIPRED
                     LIRRTDFEEETIDEVRAILASEFEE"
     gene            complement(21857..22324)
                     /gene="rimP"
                     /locus_tag="PKH68_00810"
     CDS             complement(21857..22324)
                     /gene="rimP"
                     /locus_tag="PKH68_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNX11503.1"
                     /translation="MILKDTVIQLVEAFLENTGYYLVDLKVSNDNRILVEIDSFEGVS
                     IDFCAELNRHIEANLDREIEDYELEVASAGLTEPFKVLKQYEKNLGNEVEVLTKDGKK
                     LTGILMLCDDAKIILQQEKLVKPEGAKRKTTIVEETEIPYNNIKTTKYIIRFK"
     gene            22514..23233
                     /locus_tag="PKH68_00815"
     CDS             22514..23233
                     /locus_tag="PKH68_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX11504.1"
                     /translation="MGRAFEYRKATKMKRWGNMARVFTKLGKQITIAVRESGPDADTN
                     PRLRVLMQQAKKENMPKDNVERAIKKATSRDEADYKEMVYEGYGPHGIAIVVETATDN
                     PTRTVANIRSYLNRHGGSLGTSGSLEFLFDHKSVFHIAKKEGVELDDLELELIDFGVD
                     ELVEDEDEIVLYGEYTSYSAIQKYLEEHGYEITTAEFERIPNDLKHLTEEQRASIHKL
                     LEKIEDDDDVQNVFHNMAEED"
     gene            complement(23289..24107)
                     /gene="panB"
                     /locus_tag="PKH68_00820"
     CDS             complement(23289..24107)
                     /gene="panB"
                     /locus_tag="PKH68_00820"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNX11505.1"
                     /translation="MSVHSEDTRKVTTYRLMEMKKRGEKISMLTAYDYTMASIVDQAG
                     TDVILVGDSASNVMCGNTTTLPMTLDQMIFMGKSVAKAVQRALVVVDMPFGSYQGNAD
                     IAVASAIRVMKETHADCIKLEGGEEIAESITRILTAGIPIMGHLGLMPQSINKYGSYT
                     VRAKEEAEVEKLLRDAHFLEDAGCCSLVLEKIPAKLAEKVANELKIPVIGIGAGGGVD
                     GQVLVVHDMLGLTKGFSPRFLRRYANLADTMDAAIKNYVADVKSADFPNESEQY"
     gene            24388..25161
                     /locus_tag="PKH68_00825"
     CDS             24388..25161
                     /locus_tag="PKH68_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNX11506.1"
                     /translation="MIKAIFFDIDGTLVSFETHRIPPSARNAIKGLQQKGIKIFVATG
                     RHTSAINNLEDIRFDGYISINGGYCTVENNKCIYKNHIPKSDIEALVDFQKKNPFPCL
                     IMEEEEMFINYHNRDVDHMLKMLDFPKIKVRALRESLHSDVLQVVSFFTEDREKDIMA
                     ILPGCESTRWYPLFTDIIPQGSHKGVGMDKILAYYNILPDETMAFGDGGNDMTMLRQA
                     GTGIAMGNAEDKVKAIADYVTDTVDAHGIYKALKNFGVL"
     gene            complement(25170..27356)
                     /locus_tag="PKH68_00830"
     CDS             complement(25170..27356)
                     /locus_tag="PKH68_00830"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX11507.1"
                     /translation="MRKLLLFLFSFLIFLSPAISQRKKVGVVLMGGGAKGFAHVGALK
                     IIEEAGIPIDYIAGTSMGAIVGGLYSIGYDARALDSLIHKQDWMHLLTDDVYRYNLSG
                     SEREKAQNYIFTINYAKEGIKLPSGIVEGQNILNLLLDVTTGYHEEMNFSKLPIPFSC
                     VAADAKTGKEVVFTRGSLPIALRSSMAIPGIFSPVKLDTMLLVDGGILNNYPVDVVKR
                     MGADIVIGISFEKDEKKLEKDAGSVLELTHQWENFLGKEKYEENLRNTNLAIRINLGK
                     YNSASFRMDEIDSILLKGETTARKKWDELMELKNNLGISDRNLTPQIHNPYIASDTLL
                     LRSVHIEGLSATDEQLVLKRVHIKPKITRSELQNTVAQLYGTDLFSRVHYRLENTRPP
                     YDLVLEVEKKNIRNLNVGIRFDTQDLASILLNTTIRLNTSLNSTFGVTARLNKNPYLM
                     VDYSLNRTVLHKGGVKIKTAKNDINIFDKGKLAYNLGFYQNSINVNFSEFYFYNIKLQ
                     LGANIDHFYYLYELKNARQPELNLETKPYINYLLSGSYDNLNNINSPTKGMYFNFKYA
                     ITTNNFYQLEGKNPMQAFSFTFFKPIEISRKFTLSPHIDGRIIVSDTVPFIYSNLVGG
                     QWDNQYVNQQISLQGLQGMEIFKKSVVKTQVNLKYNINKNHGVQLALNYTLQHDNPLQ
                     ILKGNAIFGGSLGYFYNTLIGPITLRLNYSDRTKEIYPYVSAGYYF"
     gene            complement(27483..28448)
                     /locus_tag="PKH68_00835"
     CDS             complement(27483..28448)
                     /locus_tag="PKH68_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNX11508.1"
                     /translation="MSEEIITDNHWLVITNPKAGIRKLPKQREFIIHTLNQAGIPFIY
                     KETEYSGHAIEIAKHYTRRGCVNFLVLGGDGSISEVINGIFAANTGDTSGIRIALIPR
                     GTGNDWGRFWKLRKNDKKSMSVFLQNNVQLIDIGKIDYVVDGVPDTRFFINSVGFGLD
                     GEVTNLTHRLKKYTGSFSFLYTIALLAAVFRYKSTDADITINRIKHSLKLFTMNIANG
                     CFSGGGIRQNPSALPYDGVFDMIVVEKPTFKDIITALPLIFNGKITKHPVIKSYRTDV
                     IGVKCSESIQVEADGIIVPGAHNCTVSILTDAIRMIVPKSELSRS"
     gene            complement(28642..>29628)
                     /gene="aroC"
                     /locus_tag="PKH68_00840"
     CDS             complement(28642..>29628)
                     /gene="aroC"
                     /locus_tag="PKH68_00840"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNX11509.1"
                     /translation="AGIELDEAFIQQELNRRRPGQSIISTPRSEEDNVEFLSGIFEGK
                     TTGTPIAFVIWNKNQESKDYNHLKDVYRPSHADYTYQQKYGIRDHRGGGRSSARATAG
                     WVAAGAIAKLVLKQQGVDITAYTSQVGHIAVGKSYENLNLSLIESNIVRCPEPATAQK
                     MIAYIDELRKDGNSIGGIISCVISGAPVGWGEPVFDKLQARLANAMLNINATHGFDYG
                     RGFEGVHLTGAEMNDAFIKEGERVTTKTNNSGGIQGGISNGEDIYFRVLFKPVATISI
                     KQNTLDKDLNAVELEAKGRHDPCVLPRAVPIVEAMAALTLVDLYLMTGPLTP"
BASE COUNT         8239 a         6110 c         6315 g         8964 t
ORIGIN      
        1 ggatggtgag cctttttggg gactcgaacc ccagacctat tcattacgaa tgaattgctc
       61 taccaactga gctaaaaagg cttgtttgtt ttcgcgtgca aagatagact ttttttggaa
      121 taatgtaaaa aataaattct catttttcgg attatacttg cttagtaacc gttgaaaggt
      181 gttcgaagcc acaatccccc gaatgttttg gttcattttc gattattggt ctttcagact
      241 gttgtagtat ttcactacct taataagatt ttctttgcgg attgatttta ttttttgttg
      301 acggaagaaa tgctccatgt aaggctcctt gtcggcaagt gcatgtctca ccgatttgag
      361 gtttacaatt ttgtaagcct gattgccgtc caccaccaga taccagctgt cgggctccgg
      421 gataaattcg gcaggaacag taccgataat gggtttagca tattccaccg gcttaaatcg
      481 gactgttcgc cgctgaagca ggtgacactc accggtttcg agcagctcat aaaagccgcc
      541 tttaccaata aatggcagat atacgtaggt ggaatttcca atgaccactt tgttcagaaa
      601 tccgggattg ccgatggcgt aagctttacc atctgcctca tattcaattt catccgtaga
      661 aatattgtag cgcagcggag cactgactac tacggtatct ttcatatata gttttcccgg
      721 agtaaatttt gggttttggt atggacttcc tttaatatct ttatagtcaa ttacattcct
      781 ggcgtgcagg ttgttctcat agtaacctgt gaaaaaatgt tcggaataaa tggtgggcgt
      841 agtttcccat tgagccaaca gattgaaagt agggaagagt aataggataa ataaaatgat
      901 tttgtaagtt ttcatatgcc aatatgttag tgaagtaacc tttttgtcca aatattgcgg
      961 tataaagata ctaaaacact ggaatattta ttatcctgtt ggcaattaaa atctaatcgt
     1021 tccttaatta tttcttaaac agctttcagc ccgttgtaaa aacgaaacaa aatccgtact
     1081 tttgcacaaa attgaggata aaatgatgat aaatgttcga aattcgaata aaaatgcata
     1141 attgcatata aaaatcattt tttccttttt tttgagtaaa aaaatctaac actcaatata
     1201 catacaatgc atcacaaacc ccttcatctt gttctggaag acggaacagt atttaaagga
     1261 aaatcatttg gttatgaaaa gcctgtagcg ggcgaagttg tattcagcac cggaatggtg
     1321 ggttacaccg aaagtttgtc ggacccctcg tactccgggc agatactcac cctcacttat
     1381 ccgctcatag gaaattacgg cgttcccaag gatgaaatta tcaacggcat ttcaactttt
     1441 tatgaatcgg aaaaaataca ggcagcgggt cttattgttt ccgatttttc gtttgaacac
     1501 agccactgga acgcatccat gagcctgagc gactggctca aagaaaataa agttccggcg
     1561 gtttttggta tcgatacgcg cgaactcacc aaactggtac gcgaaaaagg taccatgctc
     1621 ggtaaactcg tttttcccgg cgaagaagat attgagtttg tcaatcccga tgacgaaaat
     1681 caggttgcca gggcgagttg caaagaagta atcacttatg gcaggggaaa aaacaaagtg
     1741 gtgcttgtgg attgcggtgt gaagcacaac atcattcgct gtctgcttca gcgggataca
     1801 accgtggttc gggtgccctg gaattatgat tttaatgccc tggaatatga tggattgttt
     1861 atttccaacg gtcccgggaa tccggcatac tgcgagcaaa cggttgaaaa tatccgcaca
     1921 gcgatggaag ccgacaaacc gatttttggt atctgtatgg gtaatcagtt gctttcgtta
     1981 gctggtggag caaaaacata taaacttaaa tacggtcacc ggagttgcaa tcagcccgta
     2041 cagttagcgg gtacccatcg tgctttcgtt acttctcaaa atcatggttt tgcagtggat
     2101 aattccacgc ttacagccga ctgggaaccg ctattcatca atatgaacga cggaacaaac
     2161 gaaggtatcc gtcacaaaac aaaaccctgg ttttcggcac agtttcatcc cgaagcatcg
     2221 gccggaccta ccgataccga atttcttttc gatgtattta tccgaacgat aaatgaaaaa
     2281 aatatttctg ttccgaaatt gattgaagaa gaactggatg ccaaactggt gacaaaacat
     2341 aattacattt cggccgaaaa aggtgaaatt aaaaaagttc tcgtgcttgg ttcaggtgcc
     2401 ctgaaaattg gtgaagccgg cgaatttgat tattccggtt cgcaggcact caaagctttg
     2461 cgcgaagaag gggtggaaac ggtacttatc aatcctaaca ttgccaccgt acagacatcc
     2521 gaaggcatag ccgataaggt gtatttcctg cctgtaacgc ccgattttgt ggaacaggtc
     2581 attgaaaagg aacgtcctga tggcattttt ctatcatttg gcgggcagac cgcgctcaat
     2641 tgcggggtag cactttaccg tactaaaatt cttgaaaaat acaatgtgaa agtattggga
     2701 accccggtac aatcaattat tgacaccgaa gaccgtgaaa ttttcaatca gaaactttcg
     2761 gaaattgatg tgaaatatat taaaagtgaa gcagttacat ctcttgacga tgccctcagg
     2821 gcagccaata cgctgggtta tccggttatt gtgcgtgcag cttatgctct tggaggtctt
     2881 ggtagtggat tttgcgataa tgatgatgag ctgaaaacgc ttgtaaacaa agcattctcc
     2941 tattcgccac aggtattggt cgaaaaatca ctgaaaggct ggaaagaaat agaatatgaa
     3001 gtagtgcgcg accggtacga caactgtatt accgtatgta atatggagaa ttttgatccg
     3061 cttggcattc acacgggcga aagtattgtg gtagctccat cccagacact caccaacagg
     3121 gagtattaca aacttcgcga actgtccatt cgcatcatac gtcatattgg cattgtgggc
     3181 gaatgcaacg tgcagtatgc ttttgatccg gtttcggaag attaccgggt gatagaagtc
     3241 aatgctcgtc tgagccgttc atcggcactg gcgtctaaag ctacgggtta tccgctggct
     3301 ttcgtagctg ccaaactggg actgggttat ggacttgccg aactgaaaaa ttctattaca
     3361 aaagttacat ccgctttctt tgaacccgca cttgattatg tggtctgcaa aattccacgt
     3421 tgggatttgg gtaaattcca cggagtatca cgtgaactgg gtagcagcat gaaatcggta
     3481 ggcgagatta tgtctatcgg acggaatttt gaggaagtac tccaaaaagg acttcgaatg
     3541 gtaggtctgg ggatgcatgg gtttgtggga aataaggaat tcgaaaccgg aaatgtagag
     3601 aaggatttgg ctgtgcctac cgatcgtcgg attttcttcc ttgcgcaagc ttttcacgaa
     3661 ggatacacag tggaccgaat tcacgaactc accaaaatag acaagtggtt cctctataaa
     3721 ttgcagcaaa tagtggatat ggaacgggaa ctttcgggtt tcgataagct gagtgattta
     3781 tccgacgaac tattattgga agctaaaaag aaaggatttt cggatttcca gattacacgc
     3841 atggtggtga aaagtaatag ccacgatatg gatgatgaaa caaaaaaaac acgggaatac
     3901 cgcaaaagtc gcggaatatt cccggtagtg aagcaaatag ataccctggc tgccgaatat
     3961 ccggcattga ccaattatct gtatgtgact tacaacggtt caactaccga tgtgcactac
     4021 atgggcgatc atcggtcggt agtggtattg ggatcgggag cataccgtat tggctcttcc
     4081 gtggaattcg actggtgtgg ggtgaatgca ctcaccacta tccgaaatca gggacttcgg
     4141 tctgtaatga tcaattacaa tcccgaaacg gtatcgaccg attacgatat gtgtgaccgg
     4201 ctttattttg acgagttgac atacgaacgt gtcatggaca ttctggaagc cgaaaatcca
     4261 atgggcacca ttgtatcaac cggcggacaa atccctaata acctggccat gaaacttgcc
     4321 gctgccaata tgcgaatttt gggaacacag gcagaggata ttgacagggc tgaggacagg
     4381 aataaattct cagcaatgct cgacgaattg aaaatagatc agccacgctg gcaggaactt
     4441 accacctaca gcgatgtaac cagatttgta aaagacattg gtttccccgt tttggtgcgt
     4501 ccttcgtacg tactatcggg agcagccatg aatgtttgct acgacgaaac tcaactcaaa
     4561 actttcctcg acctcgctac cgaagtttcc aaaaaacatc cggtggtaat atccgaattt
     4621 ctggagcgtt gcaaggaaat tgaaattgat gcggtggccg aaaaaggcga aatagtggtg
     4681 tatgccattt cggagcatgt tgaatatgcc ggcgttcatt caggtgatgc caccacacag
     4741 tttcctccgc aaaaaattta tgtggaaacc attcgcagga taaaaaatat tgcccgagag
     4801 atagcttctt cactcaatat ttccggaccg tttaatatcc agtttttggc acgggataat
     4861 tatatcaagg tgattgagtg caatcttcgt tcatcgcgca gcttcccttt tgtgtccaaa
     4921 gtattgaaaa tcaactttat tgatctggct accaaagtca tgcttggaat atcggttgag
     4981 aaaccggaga agtcggcctt cgacttggat tatgtgggta tcaaggcatc gcagttttca
     5041 tttacccgct tacagcatgc cgatccggtg ctgagtgtgg atatgacatc tacgggtgaa
     5101 gtgggctgca tcggcgacag tttttccgaa gcaattctca aatcactgct gtcggtaggc
     5161 tatcgcattc cccaaaaacg gattatgatt tcttcgggta ctaccaagtc gaaagtggat
     5221 ttgctcgatg cctgcaagct cctcgatgag aaaggctatg aactctatgc aaccctgggc
     5281 acccaaaaat ttctgaaaga taatggtgta aaagcgattg ccgtgaactg gcccgacgaa
     5341 gagggcgatt acaatatcaa agagatgatt gccaacaaga aattcgattt gattatcaat
     5401 atacccaaag acctcagtcg ccgcgagctt accaacggat acataatccg ccgcagtgcc
     5461 atcgatttca atatccccct ggtaaccaat gcccggttgg cctctgcatt tattatcgct
     5521 ttctgtacac tgaaaaaaga ggatattaag ataaaaagct ggcaggaata ttaatgaagt
     5581 aagtgcagga tagaatgcgc tgagaaccaa acagaatact ttaaatgaat gagggacaag
     5641 attattgctt gtccctcatt tatttagttt tgttgttcat ttatttgtat agctttttcc
     5701 gaggcgattg tttcgagcgc aacttttatt tcatcggcca cctcatagtt ataatatgaa
     5761 agatgaatgg tgtatgaaga attgtcggaa ctcctgattt tatagttttt acctacctga
     5821 gtaatggatt gaatatctct ccaccagatt gtttttcccg gagtggaggc gtcgggtttg
     5881 agcgatattt tatcgttatt gatgacgata tatggtcttc tgattaaggc tatcacaccc
     5941 tgaatcaagt acttaaaacc aaagacagca taaaaagcaa gataagccca gtcgaactgg
     6001 tcaaaggtct tatggtttat tttgtcaatc atataataca aacctactgc tataaataca
     6061 atccctatag cagtgtgtat caggcttttg ttgtttttat cagctaaatt tctctttagt
     6121 tccatggttt tttgttttgg caagtttatt agcattgtaa gttaaaatcg tgcaccaatt
     6181 ctcagatcaa taaactggaa atgaattcgt ttagcattgt taagactggg gtaggaggtg
     6241 tagctgtact gtatttttgc aaaatactgt aaacgggagt cggtattgag attagtgccc
     6301 agttcaaact gtgtctggtg tcccatcacg ccgccggcaa tatcttctat tttgagtgtg
     6361 ttgagcagca acccatatcc cagaaaaggg gttaatcttt tatcccggtt caggttgtga
     6421 cgtaccgtcc atccgtaatt gagcatcgat atgtcttttg atgtggcgta gtcagcaaat
     6481 aatttggtgt agccaatagg tacagtgaga tagacagccg gttttttacc gtccaatcca
     6541 ctcacctgcc aaccatagtc aaaaagtgcg cccacaccgt gagtggcgct attaccattc
     6601 attacaccgg aacgatcgat cagtgaaaat acattccggt aacccgtttc aagactgagt
     6661 tgatggggta ctttgatcat tttatattca tccagagttt gttggctgaa ggtatttaaa
     6721 gtaaatatga atgttgccgt gagaaggaat acattttttt tcatacgttg tttgtttgtg
     6781 atgcagggcg aaaaaagtga aaaatattaa aatgaatttt agccggtaga tgtaactgct
     6841 taggttacca taacttttta ccgattgatt ttgttcagat taatttgtcg tttctgaaaa
     6901 ataactgatg tgtaccaatt ctgtcataga gatgtatcta taacagaaac cgggttgtta
     6961 tatcttccga aacttctgat aagtcagcac atcttcgtag ttggcgggtg ttttaatcca
     7021 gtttttcagc ttgccgtgca gttcataatc attctcaaag agctttcggc tcacgaggtt
     7081 gctttctata atctggacgt atagttggtt tattttcaga aaattaaata catactcctc
     7141 cactgttttc agactttcgg tagccattcc ccgacccgaa tattcttcag caacgtaaag
     7201 tccgagtgcg atgcgactgt tgtgaatatc aaaatcaaac aaatcgacag ttcccacagt
     7261 tttattttca attttgctca caatcatcaa ccgtatttgt ttgctttcgt aaatgtcagt
     7321 gggtgagtgg gatatgtaat ttttcaactg atatcgtgaa taaggagcac gtgtattacc
     7381 ggcaatccat atcgaactgt cgttttccca ccggtacagc agctccagat cttcgggttc
     7441 taccgccctc aatactatct tatcgttttc cagaaatttc atgttagtct gcatattaca
     7501 ataaaaaaca ttttttttat tcaaaactca ggtagggttc aattttcagc aggtcttcat
     7561 ccgaaagtga gtgcagtttc cttaattcat caatattctt cagttttccc ctgtcacgtc
     7621 tcaattcata tatttctttt gccttgctga aagtaaggta gggatgactt ttaagccttt
     7681 ccacacttgc tttgtttata taaattttcg aaatatggga tggatcagta ctaagaaatg
     7741 gttttatctg ctcaaatatt tcgggtttca tgccatacac ttcccggatt tgttccaccg
     7801 aatgataccc gcctaaaatc cggcgatagc ccagtatact ttttgcaaat cgcctgccta
     7861 tacccttcac agcaatgaga gcagtagtgt cggccgagtt taattccacg attgtggttt
     7921 tgctttcggt gaattgcata gccggttttt cggttgtttc ggcttgtgat tcttgcccgg
     7981 tagcttttct ttcggtgggc ttcatctcag ctatctgaat gtaaggcatt aactctccgg
     8041 ctttttcgct gctgataccg tacactttgc tgaaatcttc cgctttcctg aattttccgc
     8101 ctttgctgcg atatttcagt atgtttttgg caatataggg tttaagaccc aaccgaacaa
     8161 atgtggccga atcggctgta ttggggtcaa atgcaaatag ttgataatgg gaggtttcgg
     8221 atgatttttt agtgtatgat ttgtgaaaat cgggataatt gtcccattga ttttggcggg
     8281 atcgttcctt ttccgtcagg cttgctttga atttttcggc atccttcaaa aaaactgtat
     8341 tctctgtttt atcattctct ttgataaaat agggcatgag gaaggtggaa agcaatgccg
     8401 ccaatatcaa tgcaataagc acaataatgc ctctgcgctg acttttggaa aaataaaaat
     8461 aatctttcca caccataatc gggtttttaa ttaaaaggta aaccgataaa ttaaatttac
     8521 tgttaacaaa ataatttatc ggtcaacctg atgatttaat taatatgctc tggcaaatac
     8581 tacacgacgg gttgaaggtt tgccggtaac catacagaat ccgggatcaa cctcgccgtc
     8641 aacggggatg catcggatgg ttgccttggt ttcatttttg ataaattctt cagtttcggc
     8701 agttccatcc cagtgacaga gcagaaaacc gcctttttca atttccacct tgaattcgtc
     8761 gtatgaattg acttccctgg tcaccgaagt tcggaaatca accgcttttt tgaaaatatt
     8821 atgttgaatt tctttcagta aatcgctgat atactgctcc acgccttcca tagccactgt
     8881 ttctttggtc agtgtatcgc gtcgggctat ttccacggtt ccgttttcga gatcacgcgc
     8941 acccattgcc agccgcacag gtacaccttt caattcgtat tcagcaaatt tccagcccgg
     9001 ttttttagtg tcgttgtcat cgtattttac cgttataccc agtgctttga gtttcgaagt
     9061 aatttctttt actttttccg aaatggcatt cagttgttct tcgtttttgt aaataggtac
     9121 aatcactacc tggaaaggag ccagatttgg tggcaaaacc agcccgttgt cgtcggaatg
     9181 ggtcatgata agcgctccca tcaatcgggt agaaacaccc cacgaggttg cccacacata
     9241 atccagctga ttttctttgt tgacaaatgt gacatcaaat gctttggcaa aattctgacc
     9301 gaggaaatgc gaagttccgg cttgcagggc tttaccatcc tgcatcatcg cctctataca
     9361 atacgtttca atggcacctg caaagcgttc gttgggcgat tttacgccac gaagtaccgg
     9421 tacagccatg aaattttcgg caaaatcggc atacacatgc agcatttttt cagcttcttc
     9481 aatggcttcg tcgcgggtgg catgcgccgt gtgaccttcc tgccacagaa attcggtagt
     9541 tcggagaaac aggcgggtac gcatttccca acgaactaca ttggcccact gattgaccag
     9601 aataggcaga tcgcgatagg attgaatcca acctttatag gtattccaga tgatggtttc
     9661 tgaagtcgga cgtacaataa gttcttcttc gagtttggct tcggggtcaa cgattagtcc
     9721 cgttccgttt ggatcttgtt tcagccggtg atgggtcact accgcgcatt ctttagcaaa
     9781 accctctaca tgctcggctt cgcggctcaa aaaggatttg ggaataaaaa gtggaaaata
     9841 agcgttctga tgaccggttt ccttgaacat acggtccagt tcggcctgaa ttttttccca
     9901 gattgcatag ccataaggct ttatcaccat gcaaccacgc actgccgacg actctgccag
     9961 gtcggctttg attaccagat cattatacca ctgcgagtaa tcaactgccc gcgaagtcag
    10021 ctcttttagt tcttttgcca taataatatt tagagtatct tataataaac gattttttga
    10081 aaacgaaatg caaagatatg aatttctgaa gtatttgcca atatgataac ccgctaatgt
    10141 tgtgaaaaat agtgccgaca atcgcacgac cttgcttaaa aaaagcacag caatcttaat
    10201 ttaatccaag gggattacgt atggaaatta gaaaaataac tctgactgtt tccattaagt
    10261 gtctctttac tacttttatt ccaataaaaa actgtacttt tgtatgaaaa tagaatagat
    10321 tacctactat atgagatttg atgaattacc tctctgcgat gccgtattgg atggcttgca
    10381 ggctatgaac tttactgaaa tgactcccgt gcaggagcaa gccataccgg tcattctgca
    10441 gaaacgtgac ctgatagctt gtgcacagac cggtaccggt aaaacagcag catatatact
    10501 tccattgtta aataacctgc agactacgcc gcataccgac gatgcagtga atgcaatcat
    10561 catgtcgcca acccgtgagc tggctcaaca aattgatcag caaatggaag gattttccta
    10621 ctttgtacct acttcatcgg tcgcagtgta tggcggcaac gattcgcggg catgggatgt
    10681 acagcgcaat ggtctgcaaa gaggcgccga tgtggtgatt gccacccccg gaagactgat
    10741 ttctcatttg aatttatcag aaatagacct ttcacacgtt aggtatttta ttctggatga
    10801 agccgaccgt atgcttgata tgggattttt tgatgatatc atgcagatag tcaaccgact
    10861 tcccaaagag cgacagacta tcatgttttc ggctaccatg cctgcaaaaa ttcgccaact
    10921 ggccaaaaca attcttcgcg atccggtaga ggtgaaaatt gccatttccc gtccgcccga
    10981 cagcattatg cagacagcct atatctgtga agaaggtcag aaacctgcca ttatccgaaa
    11041 cctcttttcc gaaaaatcgc ccgagaaagt tattcttttc tccggatcga agctaaaagt
    11101 gaaggaaata accaaaacac ttcgcagaat gggattgtcg gtgggcgaga tgcactccga
    11161 cctggatcag gctcagcgcg acagcatcat gcacgaattc cgcaataacc gcgtcaatat
    11221 gcttattgcc accgatattg ttgcccgtgg tatcgatatt gacgatatta cactggttat
    11281 caattacgat gtgccctggg aagtggaaga ttacgttcat cggataggtc gtacggcgcg
    11341 tgccggtgaa aacggtatgt caatcacact cgtagcaccg gaggaacaat accgcttcaa
    11401 gcaaatagag gattttcttg aaaaaagtat tttcaaaatt cctctgcccg aaggtatcgg
    11461 cgaagcacca gaatataatc cggaagccaa cagacggcgt aaaaaacaat tcagtgcccc
    11521 caaaggaaat ttcaaaagaa agcatcacgg ctcaggtaaa aggaattgat gatacaattt
    11581 gaccaatttc cttacttttg ctaataattt cagaattata aaatgtcaaa atatcccgat
    11641 tttttaaagg aaggcgacga agtcagaatt atttctcctt ccggagtgat tgaccccaca
    11701 ctggtggatg gtgccattat tttactcgct tcgtggggat acaaagctac agaaggtatg
    11761 tttacacgca atgctctcgg acgctttgcg ggcaacgaac aggaacgttt taccgacctg
    11821 cagcaggcgc ttgacgatcc caatgtgaaa gccatacttt gcagccgtgg cggttacgga
    11881 ctggcacaaa tcatcgacag gatcgacttc agccggtttg tcaaacatcc caaatgggtg
    11941 attggtttta gcgatgtgac cattctgcat caggccttgc tctccacggg tattatctcc
    12001 ttacacagtg ctatggccaa gcagtttact gaattgccag caaaagccga gccactggtt
    12061 atgattgaaa atatactccg tggtgatatt ccatcttaca aaatcacgac caataaacac
    12121 aaccggtcag gtatcggtaa agggaaatta atcggaggaa atttgtcggt tttcatggga
    12181 atgcggggta ccggttatga tttgcccttc aaaaattcca ttttattcat cgaagatatt
    12241 ggtgagaaac cctatcagat tgaccgtatg atgcaaaacc tgcgcatcag tggcgcattg
    12301 tcacagttat cgggattgtt gatcgggcag tttaccgaat acgaagaaga tcccgaaatg
    12361 aataaaaccc tgtttcagat cattgccgat gcggtgaaag agtacaatta tccggtctgt
    12421 tacaattttt cggcaggtca tgttgacaat aattttcccc tgttgatggg tgcaaatgcc
    12481 gaactgaaag tcactgacag aggagcttca ctgtcatttc aaaccacatt ctggaaaaaa
    12541 gcaggaaatt tattaaacct gtaatttttt cagataaata aatacaggtt agtaacttat
    12601 tattaattaa taaaataaat agaaatatgt tttcaggaat agtagaagaa tcggccactg
    12661 ttgtagcatt agtacccgaa caggaaaacc tgcatctgac tttgaactgt tcgtttgtca
    12721 acgaattgaa aattgaccag agtgtatcac acaacggagt ttgtctgacg gtagtgagca
    12781 aaactgccga tacttacacc gtaacagcca taaaggaaac gctggagtgc agcaatctgg
    12841 gtaaattgaa agttggggat aaggtaaatg tggaacgcag catggtgatg aatggtcgtc
    12901 tggatggtca tatcgttcag ggacatgtag atcagactgc ccggtgtacc cgcatcgatg
    12961 aagccgatgg cagctggtat ttcaccttcg aatatgaatt tgatcaggcc atggcccgca
    13021 aaggctatat gacggtggac aaaggctcgg ttactgtcaa cggtgtaagt ctcacggtgt
    13081 gcaatccgac caacaatagt tttcaggtgg ccataatccc ttacactttt gatcacacca
    13141 attttggtca gattgctgtt ggaacttacg taaatattga gttcgacatt atcggcaaat
    13201 acatcagccg aatgctcgaa tttaataaat agcggcactt cagactattt acagcacaat
    13261 tttgaactat cctcaattta ttttgttcta cgaataaatt gaggatttat tttgtcctta
    13321 aatagagttt atcacgttga ttgttactgt tgaaaatgga cgaattaaaa aagcaaatca
    13381 aagactcttt ttttaatcca atactatact ttttgccgtt actgattttt tttgtagtgg
    13441 atgatatgtt tggattaaat atggcctgga aaatttcctt ccccgttgct ctgggtcttg
    13501 tgttttacgt ttacctgagt tataaccgct tgtttttgtg gcatttaatg ctttcggtag
    13561 gatatgttct tatcggactt attgtcagtc ttattcccgc caggtatgaa atgagaggat
    13621 tatatcagtt tgccgacgaa tttttattcc tcgggatgat gtgctttttt attttccgaa
    13681 agtcattcct ggaaaaagtt gcaagcaaag ccttaccata cgaaatgccg atgtcaaata
    13741 acgtgaatga actttatcgc atcgttaagt atcttatggt tatcgtttca acgtatatgc
    13801 ttgtccgggt tttgtacttt tttggcatta tcaatatgac tgaaaatcag ttgcaggata
    13861 tccaaatggt ttattactat tgtttcattg tgttgggact ttacgaaacc attcgtgtgc
    13921 ttatcgtacg ttatcggttg ctacacgaag actggcttcc gataattgac gataagggaa
    13981 aggtgacagg aagcagtcag tattttcatg atcttacaga aagaaaacac atgcatccgg
    14041 tggtcaggct gcattttatt gacaatggtg ttatctttct tcggcaacgg aaatcccatg
    14101 acaacactga tgcctcactt tgggatacac ctgtgagccg ccatgttcgt ttgggcgaat
    14161 cggtcgaaca gagtttacga tataaatcca aacaatacta taacatcgaa ccaaagaaat
    14221 tctttttcct cacaaattac aaacttgaga ataagtccga gtatcagttt gtatatctgt
    14281 ttgtgttatg caaaatagat ggcattgaac tcaataagga taaagtacag gctaccaaat
    14341 ggtggactgt aaatcagatt gaggataatc tcaacacagg tattttcacc gaacgattta
    14401 taaaggaatt tgaattgctc aagaggagcg gactgctcga acacgaaagt tgtaattgtg
    14461 aatgtgcact gaaaggcatg attaagagcc gtttaattga cggcaatacc ggaagaggcg
    14521 cacagaattc agcctgctaa ggtttcactt ttattttcgt gggaaatgat aatatatata
    14581 tgcttacacc caaagcaata cccgcaagca ttacctgcaa ataccatttt tcattgacaa
    14641 caaaaaaaat gctgtacaac attaccaacc acatggtgct gatggataaa atctttatat
    14701 taagtggtat ggacttatct tctctgaaac tgcgaatata ttcaccaaat atcttgtgat
    14761 tcaagagcca gtcatatagc ttctgagaac tgcgggcata aagagcagct gacaaaagca
    14821 gaaaaggggt tgttggcagg acaggcaaaa aaacgccaag aatagctaaa cccaagcaga
    14881 tggaaccgag tatgacgagt aatatttttt tcattcagcg tgcaaagtta taaaaataac
    14941 ggattgtttt gcttcccttt ttattttatc tttgtactcc caatttaaac atttcaaaat
    15001 aattaataac caataaaaca gacaccaact atatggctga tgataaaaaa gtgattttct
    15061 cgatggtagg agtgagcaag ctgtatccac ctcagaaaaa agtattaaac gatatctatt
    15121 tatccttctt ttatggtgct aaaataggta ttatcgggtt gaacggttcg ggaaaatcta
    15181 cgctgatgaa aattatagcc ggcgtcgaaa aatcatttca gggcgaggtt gttttctcgc
    15241 ccggttactc cataggctat ctggaacagg aaccggtgct cgaccccaac aaaactgtaa
    15301 aagaaattgt acaggaagga acgcaggaag tggtggatat gctggccgaa tttgaccaga
    15361 ttaatgagaa gtttgccgat cccgatgctg atttcgataa actgataatc cgtcagggcg
    15421 aattgcagga attgctcgat catgccgatg catggaatct cgacaataag ctggaccgcg
    15481 caatggatgc tctgcgatgt cctcccgagg atgcactggt caaaaacctg tcgggtggtg
    15541 aacgccgccg ggtggctttg tgccgccttt tgctccgtca gcccgatatt ttgctactcg
    15601 acgaaccgac caaccacctc gatgccgaat cggttgaatg gctcgaacat tacctgcaca
    15661 actatgccgg tacagtcatt gccattactc acgaccgtta ttttctggat aatgtagccg
    15721 gttggatact tgaactggac agaggagaag gtattccatg gaaaggaaac tattcatcgt
    15781 ggctggaaca aaagactacg cgaatggcaa tggaagaaaa acaggtcagc aaacgccgca
    15841 agaccctgga acgtgaactg gaatgggtga gaatggcccc caaagcccgc caggccaaag
    15901 ggaaagcccg tttggaagct tacgacagat tattgaacga agacgaaaaa gaacgcgaag
    15961 ataaacttga attattcata cccaacggac ctcgtctggg aaataaagtg gtcgaggcta
    16021 ttgacgtttc aaaagcattc ggcgataaat tgctgttcga gaatctcaac tttatgctgc
    16081 cacccaacgg aattgtggga ataattggtc cgaatggtgc cggtaaaact acgttgttcc
    16141 gcctgatgat ggggctcgaa aaagtggaca aaggaacttt tgaaaccggt gaaaccgtaa
    16201 agattggtta cgtggatcaa agtcacaccg atatagatcc tcagaaatcg gtatatcagg
    16261 tcatttcggg cggaacggaa tttatacgcg tagcaggtaa agatatcaat gcgcgggcgt
    16321 atctgtctcg ttttaacttt acaggtggtg atcaggaaaa actctgcggc gtactatccg
    16381 gaggtgaaag aaaccgcctt cacctggcac tgacactcaa atcggaagcc aatgtacttc
    16441 tgctcgacga acctaccaat gatatagacg ttaatacact aagggcattg gaagagggac
    16501 tcgaaaattt tgccggttgt gccgttgtta tcagtcacga ccgctggttt ctcgaccgca
    16561 tctgtaccca tatcattgcc ttcgaaggag actcacaggt atttgtattc cagggaggat
    16621 ataccgatta tgaggaaaat aaaaagcaac gattgggcga tagcggcgaa cctaaacgca
    16681 tccgatacaa aaagttgata gtataacgaa taaaaaaaaa tccatcaaaa tatgatggat
    16741 tttttttgtt attcaatgtt gaaagccagc gtaatcatcc gggtggtgag ttttgaaatg
    16801 gcatccgttg gtcccggacc actcgcaggt gctgtatctc tcatagctgt aggggtaatt
    16861 acattgctga caccaactgc aaatttcaaa acgggtgaca gtttgaaaaa cggaaaatac
    16921 agatcgcagc caactcctgc ttcaaccagc gcatcaaagg gatttaacaa gaccgcccgc
    16981 tctttattcc gaccccagtc catccacaat ccgccaccgg ccagcacata cggacgataa
    17041 ttgtcttttc gctcggagct atattttata tacaatggta aatataaggg caccgaaaga
    17101 atctgagccg gatcataatt cttataattc aaggtacgtt ggctcaacat cagggtagga
    17161 gtgaagcgaa gattgaaata gcggttgagg cgaaggtcgc tgatagctcc taccgaaaaa
    17221 cccggtgcta cggcggttac acttacatcc ttattgatat cggccggcac aacagcaaaa
    17281 tccataaaat tcaaccccag tgcatagcca aaatgcagcg gtttatcatc cacatacggc
    17341 agattgccct gagcattact cccaccaata aagaaaaata ctgagaatat aactaataaa
    17401 aatttctttg aaaatatcct gttcatttat tttttagttc gtttgatttc aaaacgccga
    17461 atcaacttgt ttattgtaat acagcagttt aaaacaaaca aattttcacc ggataaggat
    17521 gaaaatttgt tagattttgt ttactgaaac ccgatttatt tcagggtttg ttttacttct
    17581 atctcttcgt aactttcaat catgtcgccc acctgtatat cgttgaaatt tttcaggtta
    17641 agaccacatt caaagtttac gcccacttct ttcacatcat ccttgaagcg tttgagcgaa
    17701 tccagatcgc cggtaaatac tacaatgcca tcacggataa gatgcacttt gttgttgcgt
    17761 tttacttttc cttcacgcac aatacaaccg gccacagttc caactttggt gattttgaaa
    17821 acctccagta cttcgatggt agctgtaacc acttccttga tttcgggcga aagcatacct
    17881 tccatggccg attttatctc ttcgatagca tcgtaaatga tggaataaag ccggatgtca
    17941 atttcctctt tttcagctaa ttttcgtgcc gatacacttg gacgaacctg gaaaccaaca
    18001 ataattgcgt tggaggctgc agcaagaaga atgtccgaat cagaaatagc tccaactgct
    18061 ttatggataa cgttaacctg tatatgtggt gttgatagtt tgagcaatga gtcggagaga
    18121 gcttcaattg aaccgtctac gtcacccttg acaataatat taagctcttt aaagtcgccc
    18181 aaagcaatac gacgaccaag ttcgtccaga gtaaagtgtt tcttggtacg aatgctttgt
    18241 tcacgttgaa gttgttcgcg cttgttggct atttcgcggg cttcctgttc gctttccacc
    18301 acgttgaagg tgtcaccggc ttgcggagct ccgttcaaac ccaggataag tacgggttca
    18361 gagggttttg cttcattcag acgctgattt ctttcattga acatggcttt aattttacca
    18421 tgattaatac cggctaatac gatatcaccc tgacggagtg taccattttg taccagaaca
    18481 gtggcaacgt atccgcgacc cttatccagc gatgattcga taatggaacc aatcgcccga
    18541 cggttaggat ttgctttcaa atcgagcatt tcggcttcaa gaagcacttt ttcaagcagt
    18601 tcctttacgc caagtccttt tttggccgag atatcctgtg actggtattt accgccccat
    18661 tcttccacca gataattcat gttggcaagc gtttccttaa tcttttctgg gtttgcgccc
    18721 ggtttgtcta tcttattgat agcaaataca atagggacat tggcagctac agcatggttg
    18781 atggcttctc tggtttgagg cattacgtca tcatctgccg ccactatgat aatggcaata
    18841 tcggtaatct ttgcaccacg tgcacgcatg gctgtaaatg cttcgtgacc cggagtatcc
    18901 aggaatgtaa ttcgctgacc gttgtcaagt ttcacattat aagcaccaat atgctgggtg
    18961 atacctccgg cttcaccggc tattacattt gttttacgga tatagtcgag caacgatgtt
    19021 ttaccatgat ccacgtgtcc catcaccgtt acaatcgggg aacggaattc gagatcttct
    19081 tcgctgtcag caccttctga ttcggctaat gcttctatca catcctggct tacatattcg
    19141 gtcttgaaac caaactcatc ggctactatg ttgatggttt cggcatccaa acgctgattg
    19201 atggatacca tcatcccaat actcatgcag gtacggataa cctcattgac agagatgttc
    19261 atcatggtag ccaattcgct taccgttacg aattcggtaa gtttcaatac gttttcttcg
    19321 ctcttatcgc ggtcaaattg ttcctgaagt ttggcactta ctatatcgcg cttgtcacga
    19381 cggtattttg aaccttttcc ccgtttgttg ttggcttttg aaagccgtgc taatgtttcc
    19441 tttatttgtt tttgaacatc ttcttcgtta actaccgttt ttattggttt tttgaaatgt
    19501 tcttttttgg tagcttgttt tttgctgaag tcaagcggtt ctggcttatt gatatctacc
    19561 cgctgggtgt ttatcctttt cctttttgct cctttggcat cggttttgac ttctgctttt
    19621 ggctcaattt tgacatcttt gaccggttca ccttttttgg gtttgccatc cacgtagttt
    19681 ttcagacgct catcacgttc ttttttcttt tgctcctttg ttttggattt tggcctggta
    19741 ctctgattaa ggctgtccag atctatggaa ccaattacaa caggtgtggg agtaattcca
    19801 cgttttccaa tccggtatat ttcctcctct ttttcgtctt cctcctcttc ctcctccaca
    19861 ttggttgttt ctataacggg tttgggtgca acaggttttt cttcttcctt tttctttatt
    19921 tcaataatcc gcttttcagt tgcaggctca gtaattactt cttctttgag tttactttca
    19981 cttgattcgg gcggtgtagt tgttattgtc tctgtttgga ctacttttac ttcaggtatt
    20041 tcaaccggtt tttcctcttt tggaagagcg tcggatgccg gtttaatgtc tggctgcgcg
    20101 acaacaggag ctgcttttgg ctctgcaacc ggttcgggtt tttgaacttt tggagtagct
    20161 ggaggagttg gttttggttt atctaaatca atatgtccaa cttctttgat atgcggacgc
    20221 agatcgctcg aaacagtggt cgggatcatc tcttcctttt tctccggttt cttctgaaaa
    20281 ccttccagtg caattgtttc agctttttct ttggcctgac gttcctggct taaacgctca
    20341 gattccaact tcatagccat gtctttgtta aactctttgg cgagcaacaa atgttcttca
    20401 tcgctaatct ttgaatttgg gtctgcagct attttgtgcc cttttttggc aaggaattcc
    20461 actgccgtag ccattcccac atttaaatcc ttacaagctt tacttaatct gatagacata
    20521 tttttgaatt acagtttttt ttctaaaata attatttcaa aacagcaggt ctgaaatagt
    20581 ttggtgcttt ttattttttt tattcttcga attcggacgc taatatagcc cgcacttcgt
    20641 cgatagtttc ttcttcaaag tcggtacgac gtatgaggtc ttcacgggga atagacagta
    20701 cgctttttgc ggtgtcgcaa ccgatagctt tcagggcatc tattatccac tgatcaattt
    20761 catcggagaa ttcatccagg tagatatctt cttcgttttg agtttcatcg atatcgcgga
    20821 atacatccag ggtgtatcct gtaagcatac ttgccagttt gatgttgaga ccgtttttac
    20881 cgatagcctg cgaaacttct tcgggtttca ggtaaatttc agctttcttg tcagcttcat
    20941 tcaaccgaat tgaggtgatt ttggcaggac taagtgagcg tgcaataaat aggcttaagt
    21001 tattggtata attaataacg tcgatatttt cgttgcgcag ttcgcgcaca atgccgtgaa
    21061 tacgtgaacc ctttacgcct acgcaggcac ctaccgggtc aattctttcg tcgtatgact
    21121 ctacagctac tttagctctt tctcctggga tgcgggcaat ttttttgatt gtgatcagtc
    21181 cgtcgtgtat ctcgggtact tcgagttcaa acagtcgttg gaggaaaacg ggtgaagtac
    21241 gtgaaaggat gatttttgga ttgttgtttt tattttcaac cattgccacc actgcgcgta
    21301 cggtttcacc cttgcggtaa aaatcggtgg gaatttgctc tgctttaggc agataaagtt
    21361 cattaccgtc ctcatccatc agcagcattt cctttttcca gatctgatat agttcagcac
    21421 ttactatttc gccaactttc tcgctgtatt ttacgtaaag gctgtctttt tgaagttcca
    21481 gaatttttga cgccaacgtt tgacgtaatg taagaatggc acgtcttcca aaaccaaaaa
    21541 aatcaaccgt atcggttact tcttcgccca attggtagtc ttcatctatt ttctgagcat
    21601 ccgacagcct gatttccatg ttttcgtttg tcagttcgtc atcggctaca acggtgcgat
    21661 tgcggtaaat ttcgaagtcg cccttgtcgg ggttgatgat gacgtcaaaa ttttcgtcgg
    21721 ttccgtaggt ttttgaaatc acgttgcgga aagattcttc cattacactg ataagagtac
    21781 ttctgtcaat gtttttcagt tctttgaatt cggagaaggt atcaatcaaa ttgatgtttt
    21841 cttttttgct catggcttat ttgaatcgga taatgtattt tgttgtttta atattattat
    21901 atggaatttc agtttcttcc acaatagttg ttttgcgttt tgcaccctct ggtttcacca
    21961 gcttttcctg ttgcagtata atttttgcgt catcacaaag catcaaaatt cctgttagtt
    22021 ttttaccatc tttcgtaagt acttccactt cgttaccgag gtttttttcg tattgtttca
    22081 gcaccttaaa aggctcggta agtccggccg aagccacttc cagttcataa tcttcgattt
    22141 cgcggtcaag atttgcttct atatgacggt tcagttcagc acagaagtca atagaaaccc
    22201 cttcgaatga atcaatctct accaaaatgc ggttgtcatt tgatactttc agatctacaa
    22261 gataatatcc cgtgttttct aaaaatgcct caaccaactg tattacagtg tcttttaata
    22321 tcattttttt tggattttat aacaaagaag gggacttttt tgtccccctc acgttgctct
    22381 atttcggatg caaaagtacg aataattttt taattcacaa tagattatcg aaaatgattg
    22441 ataaagatat tgaattttca gtcaattttt ctacttttgc agtctgaaat tacaaacata
    22501 ttattttatt tatatgggaa gagcatttga atacagaaaa gctaccaaaa tgaaacgatg
    22561 ggggaatatg gcccgcgttt tcaccaagtt aggcaaacaa attactattg ctgttcgtga
    22621 gagtggcccc gatgcggata caaatccccg attacgcgtg ctgatgcagc aggctaaaaa
    22681 ggagaatatg ccgaaggata acgtggaaag agcgataaaa aaagcaactt cgcgcgatga
    22741 ggccgattat aaggagatgg tgtatgaggg ctatggaccg catggcattg ccatagtggt
    22801 tgaaacggct accgataatc ctacacgcac agttgccaat atccgcagtt atctgaaccg
    22861 tcacggtggc tcactgggca cttcaggttc gctggagttt ctttttgatc ataaatcggt
    22921 atttcacatt gccaaaaaag agggtgtgga actcgatgat ctggaactgg aactgattga
    22981 tttcggagtg gacgaactgg tggaagatga ggatgaaatt gttctttatg gtgaatatac
    23041 ctcttattcg gctattcaga aatacctcga agaacatggt tacgaaatta ccaccgctga
    23101 atttgaacga attccaaacg acctgaaaca cctgactgaa gaacagcgcg cttcaatcca
    23161 taaattactt gaaaagatag aggatgacga tgatgttcag aacgtatttc acaatatggc
    23221 ggaggaagac taaattttct accggaataa aaaaaaggaa agcgtatgct ttcctttttt
    23281 ttattcattt aatactgttc gctttcgttg gggaaatcgg ctgattttac gtcggccacg
    23341 tagtttttga tggcagcatc cattgtgtcg gccaggttgg cgtaacggcg taaaaaacgg
    23401 ggcgaaaatc ctttggttag tcccagcata tcgtgaacca cgagaacctg tccgtcgaca
    23461 ccacctccgg caccgatccc gattaccgga attttcagct cattagccac tttttcggcc
    23521 agttttgcag gaattttttc gagtaccagg gaacagcatc cggcatcttc gagaaaatga
    23581 gcatccctga gcagtttttc aacttctgct tcttctttgg ctctgactgt gtacgagcca
    23641 tatttgttga tagactgggg cataaggcca aggtgtccca tgatgggaat acctgctgtg
    23701 agtattctcg taatggattc ggcaatctct tcaccgcctt cgagtttgat gcagtcggcg
    23761 tgagtttctt tcatcacccg gatagccgaa gctaccgcaa tatcagcatt accctgatag
    23821 ctcccgaaag gcatatccac taccaccaaa gcccgttgaa cagctttagc caccgatttt
    23881 cccatgaata tcatctggtc cagcgtcatt ggaagtgtgg tagtattgcc acacatgaca
    23941 ttggaagccg aatcgccaac gagtataaca tctgttccgg cctgatcaac tatgcttgcc
    24001 atggtgtaat cgtaggcggt aagcatactg attttttcgc cccttttttt catttccatc
    24061 aggcgataag tggttacttt tcgcgtgtct tcgctatgta ctgacatatt cttatttttt
    24121 agattgataa ttacagcatg caaagttata aataatattc aacttgtctt tcgtattaaa
    24181 actttaaata ctggcttact gttttttcat tggtgttgtg ttcagaaatt aattattaat
    24241 tgttttacgg gatgttttga ttaattttac caattaaata agagctttaa ataggattat
    24301 atttgttgtt ccgatgctgc tatttaaagc tataaatatc agaataataa ttcctgtaat
    24361 atataaattg aaataaatat tgattatatg attaaagcta tattttttga cattgacggc
    24421 acactggtaa gtttcgagac tcaccgcata ccaccttcag ctaggaatgc aataaaagga
    24481 ttgcagcaaa aaggaataaa gatttttgtg gcaacgggtc gtcatacgtc ggctatcaat
    24541 aacttggaag atatacggtt tgatggatat atttcaatta acggaggtta ttgcacagtt
    24601 gaaaacaaca aatgtattta caagaatcat atcccgaaaa gtgatataga agcgcttgtt
    24661 gactttcaga agaaaaatcc ctttccatgt ttgatcatgg aggaagagga aatgttcatc
    24721 aattatcaca accgcgatgt agatcatatg ctgaaaatgc ttgattttcc aaaaattaaa
    24781 gttcgtgccc tcagggaatc gctccattcg gatgttttac aggtggtttc attcttcacc
    24841 gaagacagag aaaaagacat tatggctata cttcccggct gcgaatcaac acgatggtat
    24901 ccgctattca ccgatattat tccacaggga agtcacaaag gcgttggtat ggataaaatc
    24961 ttagcgtatt ataacatctt acctgatgaa acgatggcat tcggcgatgg cggcaatgat
    25021 atgactatgt tgcgtcaggc cggtacaggc attgccatgg gaaatgccga agataaggtg
    25081 aaagccattg ccgactatgt aaccgatact gtggatgccc acggtatata taaagccctg
    25141 aaaaattttg gagtattata aaaataatct tagaaataat aacctgcact cacgtaagga
    25201 taaatctctt tcgtacggtc ggaatagttc aaccggagcg taatcggacc gatcagggta
    25261 ttgtaaaaat atcctaacga tccgccaaaa atggcattac ctttcaatat ctgcagcgga
    25321 ttatcatgtt gcagggtgta gtttagtgcc agttgtaccc cgtggttttt attgatattg
    25381 tatttgagat tcacttgcgt tttcaccacc gactttttga aaatttccat tccctgcaat
    25441 ccctgtaatg atatttgctg gttgacatat tgattgtccc actgcccacc aacaaggttt
    25501 gaataaatga aaggtaccgt gtcgctcacg attattcgtc cgtcaatatg gggagacaat
    25561 gtaaatttcc tgcttatttc aatgggtttg aaaaatgtaa aactaaacgc ctgcatggga
    25621 ttttttcctt caagttgata aaaattattg gtggtaatgg cgtatttgaa attgaaatac
    25681 atgcctttag taggagagtt gatgttgttg agattatcat aactgccact caacaagtaa
    25741 tttatataag gtttggtttc aaggtttaac tcaggttgtc gggcattttt aagctcatac
    25801 aggtagtaaa aatgatcaat attggctccg agttgaagtt tgatattgta gaaataaaac
    25861 tcactaaagt ttacattgat actattctga taaaatccaa ggttataagc gagtttgccc
    25921 ttgtcaaata tgtttatgtc atttttagct gtttttattt ttacacctcc tttgtgaagc
    25981 acagtgcggt taagcgaata atcaaccatc aggtagggat ttttgttcag tcgggcagta
    26041 accccgaaag tagaattcaa cgaagtgttc agtctgatgg tagtgttaag cagaatagat
    26101 gccagatctt gtgtatcgaa ccggataccg acattcagat tgcgaatatt tttcttttca
    26161 acttccagca caagatcata gggtggtctg gtattttcga ggcgatagtg cacccgcgaa
    26221 aaaagatcgg taccgtaaag ttgtgcaact gtattctgta attcagaacg tgtaattttg
    26281 ggcttaatat gcaccctttt aagcaccagc tgttcatcag tggccgataa tccttcgata
    26341 tgcacacttc ggagcagcaa agtatctgaa gcgatataag gattatgaat ctggggggtg
    26401 agatttcggt ctgaaattcc aagattgttt ttcaactcca tcaattcgtc ccatttcttc
    26461 cgtgctgttg tttccccttt aagcagaata ctgtcaattt cgtccattct gaaactggca
    26521 ctgttatatt tgcccagatt tatccgaata gcaagatttg tattgcggag attttcctca
    26581 tatttttctt ttcctaagaa attttcccat tggtgagtta gctccagcac cgacccggca
    26641 tctttttcca attttttctc atctttttcg aaactgatgc cgataacgat atcggcaccc
    26701 atccttttga ccacatctac cggataatta ttgagaatac caccatcaac aaggagcatt
    26761 gtatcgagct ttacaggact gaaaataccg ggaatagcca tactcgatcg taaagcaatg
    26821 ggtagagaac cgcgggtaaa aacaacttcc tttccggttt tcgcatcggc agccacgcag
    26881 ctgaaaggga tgggcaattt actgaagttc atttcttcgt gataaccggt agtgacgtcg
    26941 agtaaaagat taagaatatt ctgaccttca acaataccgg aaggtaattt aatgccctct
    27001 ttagcatagt tgattgtaaa aatatagttt tgagcttttt ccctttccga tcctgataaa
    27061 ttgtatcggt acacatcatc ggtgagcagg tgcatccagt cttgtttgtg aataagtgag
    27121 tcgagcgccc gggcgtcata tccgatgctg taaagtccac ctacaatagc acccatactt
    27181 gtaccggcta tgtaatcaat gggaattcca gcttcttcaa ttattttgag tgctcccaca
    27241 tgtgcaaaac ctttggcacc accacccatc aatactacgc caactttttt gcgctgactg
    27301 atagccggcg atagaaaaat caggaacgaa aaaagaaaaa gcagcagttt tctcatttgg
    27361 atattactga acgatatatt taaaagcatg gtgtgcatac actatatata actcaaaatc
    27421 agttgatttg ttttataatt aacaacaatc agaagccatt ttattatgaa gaaataataa
    27481 gcttatgatc ttgacaattc acttttcgga actatcatcc ggatagcatc ggtcagaata
    27541 ctcaccgtgc aattatgtgc accgggaaca ataataccgt ctgcttcgac ctgtatgctt
    27601 tcgctgcatt tcacaccgat gacatctgtt cggtacgatt ttattacggg atgtttggtg
    27661 attttgccgt tgaaaattaa tggcaaagca gtaatgatat ctttgaaggt aggtttttca
    27721 acaacaatca tatcaaaaac tccatcgtag ggcagggcag aaggattttg cctgataccg
    27781 ccaccgctga agcatccgtt agctatattc atggtgaaga gtttcaaact atgctttata
    27841 cggttaatgg taatgtcagc atctgtcgat ttgtatctga aaactgctgc caataaagcg
    27901 atggtgtaga gaaatgaaaa acttccggtg tactttttca gcctgtgagt tagattggta
    27961 acttcgccat cgaggccaaa ccctacggaa ttgataaaaa aacgggtgtc gggaacacca
    28021 tccactacat aatcaatttt tcctatatca atgagttgta cattgttctg aaggaacacc
    28081 gacatggatt ttttgtcgtt ttttctcagt ttccaaaacc gcccccagtc gtttccggtg
    28141 ccgcgtggaa ttaatgctat tctgataccg gatgtatcac cggtgttggc tgcgaatata
    28201 ccgttaataa cttcacttat tgatccgtcg ccgcctaaaa caagaaaatt tacacatcca
    28261 cgacgggtat agtgttttgc tatttcaatg gcatgtcccg agtattctgt ttctttgtaa
    28321 atgaagggaa tacctgcctg attgagcgtg tgaatgataa attcccgctg ttttggcagt
    28381 ttccttattc cggctttcgg attggtaata accagccagt gattatctgt aataatttct
    28441 tcagacattg agaggcccct ccaaacctcc ccaaagggga ggattatgct aattaaatta
    28501 aaatattata gtgaaaaaaa cgtaatatta atacaatact gatacagttg taattgatag
    28561 tgagtaagtt gtgaggtgaa actttgaaac aaaaaaataa tttctaaatt cacattatgt
    28621 tttagagtga ctttttcccc tttagggggt cagggggccc gtcatcaaat acaaatcaac
    28681 caacgttagt gcagccattg cctctactat gggcacagca cggggcagta cgcacgggtc
    28741 gtgtctccct ttggcttcaa gttccacagc attcaggtct ttatcgagtg tgttttgttt
    28801 tatcgaaatg gtggctaccg gtttgaaaag cacgcggaaa taaatatcct caccattgct
    28861 gatgccgccc tgaatacctc cggaattgtt cgttttggtg gtaactcttt caccttcttt
    28921 gatgaaagca tcattcattt cggcaccggt gaggtgtacg ccttcaaaac cacgaccgta
    28981 atcaaagccg tgtgtggcat taatattcag catggcattt gccagacgag cctggagctt
    29041 atcaaaaacc ggttcgcccc aaccaacagg agcaccactg atcacacaac tgataatgcc
    29101 gccaatggaa tttccgtctt ttcgtaattc atcaatgtag gctatcattt tctgagcggt
    29161 ggcaggttcg ggacaacgga ctatattact ctcaatcaac gataaattga gattttcgta
    29221 agatttgcca acggctatat gtcccacctg cgacgtgtaa gctgtaatat ccactccctg
    29281 ttgtttcagc actaatttag caatggctcc ggcagccacc catccggctg tggcacgtgc
    29341 tgaacttcgt cctcctccgc ggtggtcgcg tatgccgtat ttctgctgat aggtatagtc
    29401 ggcatgtgaa ggacgataca cgtctttcag atgattgtaa tccttgcttt cctggttttt
    29461 gttccaaatg acaaaggcaa tgggcgttcc tgtagttttt ccttcaaata ttccggaaag
    29521 gaattccacg ttgtcttctt cggagcgggg agtagatatg atggattgcc cgggacggcg
    29581 gcggttaagt tcctgttgga taaatgcctc atccagctca attccggc
//
LOCUS       DAOPZK010000007        29340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1575_length_29340_cov_22.538569, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000007 DAOPZK010000000
VERSION     DAOPZK010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29340
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1575_length_29340_cov_22.538569"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKH68_00845"
     CDS             complement(<1..310)
                     /locus_tag="PKH68_00845"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HNX11510.1"
                     /translation="MKPTKFLLIALCLMFNLPGRAQTIDSYFVDMPAIILPGLSSSMR
                     LELTEYYKSEGRDSIRNQFGYNVHLLALDTVNGYISLQTASNARFEMKTLLRNDTSVV
                     L"
     gene            complement(297..4622)
                     /locus_tag="PKH68_00850"
     CDS             complement(297..4622)
                     /locus_tag="PKH68_00850"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNX11511.1"
                     /translation="MSIGKVDYQFFNSFSFDSLYIQDKQKDTLLFVESGDAGFDFWEF
                     FSGKFIFKKINIDHLYGNLVIDTAGVSNLDFILKALRQTKKPKKESNVEFNFKNINIF
                     NSHFRLTNLKYPSPGDSTRFDANRMDFRQLNMKLNVDYLKKDSLSAHIRSFSAVEQSG
                     LTVRNLETYIHGWKKGFRMPYLNISLPNSILAMDSVSMTYDSIASLKDIQNKVKWTAK
                     IKPSEITLKDLSAFVPNFRYMKDAATIQGNLSGRISSFRLQKLELKYGNSLTMKTDLD
                     LNGFPNLDETFIYANIRDFHVNKGDAQDFISQLTHKPFQLPKELSRLGMVKYTGNISG
                     FFSNLVAYGNITTNAGSLNTDILLQFENNMKDLKYNGTIRTSSFQLGDLLSTKTLGKT
                     AFKINTKGTKLQNRSLQGTISGDIAEIYLNRYNYQKIRVDGKYDGSGFEGKMDVNDPN
                     LTASFDGVVDLTKKLPVFNFDLLVNNADINALHLTKEYKDSRLSFYGNTNMIGNSLNN
                     LNGYLLLDSIRFINAGEELVMDQLLFESEVSGKSSKFTITSDLINGNFEGDFKYSTIP
                     QTISSIVENYIPALSGKNIPGTWKQGGAHNYMNIDLTLSDTKLLTKVFNLPFTLDGIT
                     TVKGYVDDIHERVELIAQTPYLSYGKQKISNIALTLNNEQQKLNMQANAVLINKNSET
                     SININAAAANDSLYTQLGWQNNDTVNYAGEIQANTKFHKENNLTSALIAFLPTQIIIA
                     DTTWDVLASQLSINPDTTMDVRNFRIQNRKQYINLNGRVSRNENDILSVDMNDLNIDF
                     IMSLVKLKDISIKGNATGQVGVYSLLKKPVFDAKIFVKNAGLNGSNVGDAQLLSTWDR
                     ERQEIQASGKFVNGTDTVALANGVYIPKTDSLDFIFDAHKLNLGFLRPYLSSIAKDIN
                     GYGTGKVRMFGKSKKIGFEGNVYAENASMTIDYLKTKYSFTDMVYLTRKSIAMKNITV
                     YDEERNTGTLNGLITHDGTFQDMKYDLKIRARNMLALNTEAKDNEFFYGKAYATGNVN
                     IYGNLTNSYVDVNATTQPKTKVFISVGGAAIANENNFITFVNQKDTTKTESAAIAQTK
                     AKPSRLRMNLMVDVTPDADIQLIIDPKAGDMLSGTGSGSLRMEYDDYSDIKLYGTYTI
                     DEGNYLFTLQNLIRKDFKINQGSNISWTGDMLRAQMNINAIYTLTASLRDLMNDAILS
                     STSRTSVPVNTVLKLTGDLMSPDIKFDIDLPTSDETLKMQVRNLINTEEMMNRQIMYL
                     LLFNKFYTPDYSKGVTTFATSLGTNTMSLFANTAFGQINNWLSQMSNTFQLGANIRST
                     GVGTQAAQEYEAEISYQPNNRLLVNGNLGYRNDDLAKNKIIGDVDVEYILTENGKLRL
                     KAYNHTVDRYTLRSAPFIQGVGLMYKETFNTWDELIRHYWRVISKPVPIPVDTTTIKP
                     TDETN"
     gene            4966..6000
                     /gene="tsaD"
                     /locus_tag="PKH68_00855"
     CDS             4966..6000
                     /gene="tsaD"
                     /locus_tag="PKH68_00855"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNX11512.1"
                     /translation="MSENITIIGIESSCDDTSAAVIRDGVLLSNVIAGQAVHTRYGGV
                     VPELASRAHQQNIIPVVHDAMLQAGIDRKDLTAIAFTRGPGLLGSLLVGSSFAKGLSQ
                     SLGVPLVDVNHLQGHVLAHFISQGHEGEKFPAFPFLCLLVSGGNSQIVLVRDYLDMEV
                     IGQTIDDAAGEAFDKCAKVMGLPYPGGPHVDRLAKEGNPKAFKLNKPQISGYDYSFSG
                     LKTSFLYLLRDEIKKNPDFVQENTADLCASLQATIIEILMKKLLKASEELNIRQVAVA
                     GGVSANSGLRNAFHDYAQKYGWEIFIPPFAFTTDNAAMIAITGYYKYLRGEFSCLEDV
                     PYARVSNLLV"
     gene            6005..6574
                     /locus_tag="PKH68_00860"
     CDS             6005..6574
                     /locus_tag="PKH68_00860"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HNX11513.1"
                     /translation="MKLTLPEKLKPYSGVINFMIILLVSHFFWKYTVLGEDDDEIVRF
                     FGLNISQPFIFMASHTAQVSYSLLKWLGFNVTLNDLNVIRHLGSGNGVHIVWSCTGIK
                     QAYILFCILAFSRGPWKHKLWYIPLGLLCVYLFNILRITFITGVIDSHPQQFEFWHEH
                     VTKYAFYAMIFGLWVIWEEKIRPQTPKGA"
     gene            complement(6707..7804)
                     /gene="buk"
                     /locus_tag="PKH68_00865"
     CDS             complement(6707..7804)
                     /gene="buk"
                     /locus_tag="PKH68_00865"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNX11514.1"
                     /translation="MHKILVINPGSTSTKFAVYEDDLQTAIYTVRHSTEEVIQYQHIL
                     DQFDFRYELIMKTLDENGVDLSEFSIIMGRGGMVPPVESGVYEVNEEMLETLRKHERG
                     EHASNLGAFLADKIARGIPGCKAYIADPVAVDELEEVARISGMPELPRRTIFHALNHK
                     ATARLHASKVGKNYEDMNLVVAHLGGGVTIGAHRKGRVIEVNQGLDGWGAISPERAGT
                     VDAGLLVRMCFSGKYTQHELMRKLAGQGGLIAHCGSNNVQELVKKAEAGDSHVALVLQ
                     AFAYSVGKEIGAMLAVLKGEVDGVILTGGVAYNDFIVDYVKAMIDPLATVYVYPGEDE
                     MAALASNALRVLQGEIPKIFKVKEGYVDYSF"
     gene            complement(8128..8664)
                     /locus_tag="PKH68_00870"
     CDS             complement(8128..8664)
                     /locus_tag="PKH68_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNX11515.1"
                     /translation="MILPVTDIKILIAYFSRRGKNYMDGKVVTLEVGNTEVLAHKIQQ
                     LMGGDLFEIRTLQEYPEEYIDTTRVARNELRTNARPELAEYVKNMEDYDVVYLGYPNW
                     WGTFPMAVCTFLESADFSGKTIIPFCTHEGSGIGYSEQDLQKLCPGAKLITGIAIRGG
                     RVASADRQLEEWLQKMTL"
     gene            9044..10843
                     /locus_tag="PKH68_00875"
     CDS             9044..10843
                     /locus_tag="PKH68_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNX11516.1"
                     /translation="MKHYPHIFFICFILLLSFGCSKTSNVPYPEPLAKAITYFYNENE
                     NEKVTDELNKLNPEKSSEEVNGMAQLFRAAALCETEKVDSAFTLLKSIKPLPKNPRFM
                     FWHNSIRGLILFRQNELLDSHKALAATTTSNYKDTRALALNERILARISFLLSDQTKG
                     IEWLTASSQHFEESGLIKSTGINLKILGRYHLNNRNYAKALQSFRSAENIFIKYNDKA
                     ELFYIYINFMDYCLVTKQLKAARYYANKSVSESNEIMDNSMRALAYNNLAEIEMTLEN
                     YDTAIHYLNHTLEIPANYSTFNIRRVYAHIKLSDIYLQKKDTLQAHQQLLAARRSLPV
                     YGYLELRHQTYARLAKTYKAINNIKSAYTSLDTAKMYLDSTFYSLTNDTKTFYDTKNE
                     LTKSAYNLENFKTTKQRNDQINLLVILGLIVLAAFVLIIYRQQREKNKVLSTLVQKNI
                     RLVEEERKLRQTQQAKTKQKNGRKTTEVDNKYQQIYEQLIQWLETDKNFSRKELTLDS
                     IAKELNTNREYLSRAISEQSLRFNDLINKYRVDEVISILSDPTNHSNKYNLAVIGNQA
                     GFNSNSVFIEAFRKQTGMNPAQFRENIENPARG"
     gene            11278..11643
                     /locus_tag="PKH68_00880"
     CDS             11278..11643
                     /locus_tag="PKH68_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11517.1"
                     /translation="MKKIILLAITLLLAGYSAYSQQTSVISEIFSRYAHQKVAERQKL
                     ILFDDAQAAELEAMEYQYLLDLQKAETGCRCRAQKRTEKLDRLRDLNLQKILTREQYL
                     KYEALDKDKIRKLPLRLTE"
     gene            complement(11699..13195)
                     /locus_tag="PKH68_00885"
     CDS             complement(11699..13195)
                     /locus_tag="PKH68_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX11518.1"
                     /translation="MIKKIGILFLLPLLLIQSSCKPKDPVADDSKLVSQFVYDGLSTY
                     YLWADEMKNKKPTVADTDPVEYFESLLNATDKDHGWSFITDDVQSLLADFAGEPKAFG
                     FSLTFAAANEARTEYYAIIKYVFPNTPASAAGLQRKDIIGKINGNAITQENYTLLYGT
                     DAASFTMYKLSGSSVVQDRIVTVTPKTIQTNPVLYNRIYEAGGKKIGYLFYTDFISNY
                     NDSLYSVFSSFRQAGVTDLVLDLRYNHGGALTAASYLCSMIAPAAVVQNKSPLVIMSY
                     NDFLNSAFDKEKVSRTNSLGVYGDKEKDPLGANLNLNKVYIIATGDSYSASELTTFCL
                     KPYIEVVHIGENTGGKYTASWTLHPYDSDIGVYIYSAASLTAAEKAKLGNWAMQPIVA
                     KYTNKSGADFSNPGYLVPDYPLKEGFGYIRNWTDIGDPADVYLGEALYLITGDINYKP
                     VPPPSLSQVKALPQAGVTLHNPRDVRREAVIIDNLKQKSLPPMQNIMR"
     gene            complement(13268..14725)
                     /locus_tag="PKH68_00890"
     CDS             complement(13268..14725)
                     /locus_tag="PKH68_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX11519.1"
                     /translation="MLQNFISERIRTELPFEPTEQQEQLIDRLGEFVMSPEPEKLFLL
                     KGYAGTGKTSVVSALVRALNILEQKTVLLAPTGRAAKVLSGYSGFPAFTIHKKIYRQK
                     SMSEFRFQLADNLHKHTLFIVDEASMISNNGTDGSFGSGRLLDDLIEYVYGGDECSLL
                     LLGDTAQLPPVEQPQSPALETGALQAYGLKVYEFTLTHVVRQALESGILYHATQLRLD
                     LAEDNISHHPKFELRDFTDISRLNGMDLIDEIQRAYNGVGVENTIVITRSNKRANMYN
                     NGIRSRVMMKEEELSNGDLLMVTRNNYFWNKPYKEIEFIANGDVLEVIRIGKHREIYG
                     FRFVDLSLRSLDYGWEIDARIWMDSLQAESPARMNEMTGKLFEAIAEDYPEITNRKLL
                     FKKILDNEYYNALQVKFAYAVTCHKAQGGQWDKVFIDQGYLPDDQINREYFRWFYTAL
                     TRASGQVFLVNFPDEWFDNKDQTKNPKEITKLKKS"
     gene            14957..17002
                     /locus_tag="PKH68_00895"
     CDS             14957..17002
                     /locus_tag="PKH68_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNX11520.1"
                     /translation="MKKIILYLMVPILFACDGKKTENVTPSVENFNYSVDKFADVQIL
                     RYRVPDFEKLTLKQKEMIYYLSQAAIEGRDILYDQNNKHNLSIRRTLEAVYENYKGDR
                     NAEAFTQLTIYLKRVWMANGIHHHYSEDKFTPEFSAAYFAETVRSIDPAKLPLQQGES
                     VDQLIAKLSPVIFDPTVYPKRTNQADGVDLILTSANNYYEGVTQQEAEDFYANMKNPN
                     DSTPISYGLNSKLVKENGKIVEKTYKIGGMYSEALSRVAGWLEKAAAVAENDQQRDII
                     NTLISFNRTGDLKTFDEYCIKWVQDLTSQVDFVNGFTETYADPLGMKASWESIVNFKN
                     IEATKRTEIISSNAQWFEDNSPVDKNFKKEKVKGVSAKVITAAMLGGDCYPPTPIGIN
                     LPNANWIRAEYGSKSVTIENITEAYDQVAMGNGFNEEFMWSDVERNRAREYASLTNNL
                     HTDLHECLGHGSGKLLPGTDPNALKAYDSTIEEARADLFGLYYMGDPKLVELGLLPDK
                     EAFKAEYYQFLMNGLMTQLTRIKPGKNIEEAHMRNRQLIANWVYENGKADNVVEFKVR
                     DGETFVVVNDYDKVRTLFGKLLAEIQRVKSTGDFAGGRDLVEKYAVKVNPELHKEVLV
                     RYEKLNLAPYKGFVNPVYALVTDKKGKVKDVTVSYTENYVDQNLRYSREYSKLPNYN"
     gene            17153..17593
                     /locus_tag="PKH68_00900"
     CDS             17153..17593
                     /locus_tag="PKH68_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HNX11521.1"
                     /translation="MKKNLILLMIVAGITACIHKSENAGEQAATDSVAVTTQVSSGQS
                     QSTWLGEYTGIFPCGDCEGIEILITLNTDSTYTQKSTYLGKGNSFEETGTFMVQEPGS
                     RLVLKSSDSEQAYLLGDGTLTLLNSEGKVTEGELAEMYVLKKKE"
     gene            17676..19724
                     /locus_tag="PKH68_00905"
     CDS             17676..19724
                     /locus_tag="PKH68_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNX11522.1"
                     /translation="MKNLIITLNIKKHFRGILLVLFPFLIFLFFLPVNLPAQTPVDYA
                     IQGIPFNTVSLNDGFWLPKIETNRTITIPSSFQKCEETGRVENFILASGAKKGKFLTV
                     YPFDDTDIYKTIEGAAFSMTVHPDKALDRYVDSLIAIVAAAQEPDGYLYTARTIDPAH
                     PHDWSGSERWVKESELSHELYNSGHLFEAAAAHFIATGKRNLLNIALKNANLLVQVFG
                     PGKRDDAPGHEIVEMGLVRLYRITGEKKYLDLAKFFIDCRGKIPNSKQFYSQNHIPFV
                     QQTEAVGHAVRAAYLYSGVADVAALTGNKEYIDAIDKIWENTVTKKFYITGGIGALHA
                     GEAFGADYELPNLTAYNETCAAIANVYWNYRMFLLHGDAKYIDVLERSLYNNVISGIG
                     LDGKTFFYPNPLESNAKYAFNYGNKLSREPWFDCSCCPTNLCRFMASVPGYIYAQKQE
                     DIYVNLFIGSKTTFKLQKTSVVLSQQSNYPWEGNVSIQVNPTKKTDFSLCLRIPGWAR
                     NQPVPGDLYRYVASTPEEIVVKVNGKAVAYTTREGYAVISRQWKKGDKVEYTLPMNIH
                     RVEANEKVKDDRGKIAIERGPVVYCLEGIDNNNNLNTIILPDNAPLSVKFQPAKLNGI
                     NEISGEAIVLEIATDGLSVQTKKQPFTAIPYYAWSNRGINQMKVWLPRKVKEITISNE
                     "
     gene            complement(19763..20380)
                     /gene="coaE"
                     /locus_tag="PKH68_00910"
     CDS             complement(19763..20380)
                     /gene="coaE"
                     /locus_tag="PKH68_00910"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNX11523.1"
                     /translation="MKTPLIIGITGGIGGGKSTFSRHLREQGWLVFDTDMEAKNLQNV
                     CPLVREKVIAEFGDEVYNANGLDRVKLAQIVFGNAEKLQKLNQIVHPEVKKKFREWAM
                     EHSRERFLFMECAILFEGSFNELVDKTVLITAPVDVRIQRVMHRDKVTEELVLTRIRN
                     QWPDEEKMKLADWTFNTDNDINPAQRVDDFLKLLEETYLENQSPT"
     gene            complement(20463..22166)
                     /locus_tag="PKH68_00915"
     CDS             complement(20463..22166)
                     /locus_tag="PKH68_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNX11524.1"
                     /translation="MKKFIFTLFILLISVAAFAQYIPQHITYTRIYDFVDELATDGFI
                     DLNSAIKPYSRDFICRKLLEARQQEDKLNSRQKQELYFFLEDYAPEQDRLPEFEFPAM
                     EKEDFRLDFYPPIFNYRDSMFRARIQPILGMNIYSNKNGRITQRWFGVDFQSMIGKNL
                     SVYGSLRDISFGGGRLSEPEYLMNFPGYEYKEPSDFSDSRGGITYGWKWGSVGLIKDN
                     IMWGDNYNGANILSGRAPSFPMISLSLKPAEWFEMNYIHGWLVSNVADSSRYYLENGV
                     KKWYRNHNKFIAANMFTLTPFRNFHFSFGNAIIYAENNVQPGYLLPVAFYKSIDHTMT
                     KGIATENQNSQLFFNISSRNIRHLHLYSSVFIDEMKFSRFKPSSAEKNPLSVKIGAKM
                     NNQFVDNLSLTAEYTRTNIINYKHSIDVLTWASNGYNLGHYLGDNAQEIFLSAGYKPL
                     RGLDISLSYLNAKHGNEYDYLRRQNGVATIGKIISQPSLGDVIWKNQTIGLKLVYELF
                     NNGYAVINLENSNIEGFDATSDPIAGERRMTATEALNYYTPTLFRGKNTTLTVGFSFG
                     F"
     gene            complement(22213..>23547)
                     /locus_tag="PKH68_00920"
     CDS             complement(22213..>23547)
                     /locus_tag="PKH68_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNX11525.1"
                     /translation="YNSIKSKSFIQSAFSLFFTSVIILVIFGSFILIQPASPYSQNVL
                     ISVLMGVFVSLYLFLFAYYLYRYAVTYDTPNLNPDKRLSAVPHPSHPMSDDAVTERYK
                     RITEFSGEKVINYLQKNTMLYESGTYIFTEFNEKELEDIEHYQYSTYIQLKRLNHIRG
                     INKMLALVNEKLPDNGILVCCYKSQSTIKQRIFDKYPRPVALTVYIFYFLYHRVMPKF
                     FLTKRLYYDLTEGKKRVLSKTEVLGRLNYCGFMLERQSKINDMNFVVARRVKNAAPLL
                     DRNYGPLIKLKRTGKNGKTFSVYKFRTMHPYAEFLQQYVYENNDLQEGGKFKRDIRVT
                     SMGRFMRKYWIDELPMLINLIKGDMKLVGVRPLSKQYFNLYTPELQQKRTNYRPGLLP
                     PFYADMPKTLDEIQASELRYLKQCETNGELRTDFKYFFLILKNILFNKARSA"
     assembly_gap    23548..23647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23648..24010)
                     /locus_tag="PKH68_00925"
     CDS             complement(<23648..24010)
                     /locus_tag="PKH68_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNX11526.1"
                     /translation="MKIINKELLDEITRQAKESPRLRMNYNLHDNLDAKAQKLLNALE
                     PGTILPIHRHPNTNETYLLLRGKLNVILYNDKKEVLETMHLDTATGNYGLDIPAGQWH
                     SVEVLESGTVILEVKDGPY"
     gene            complement(24116..25183)
                     /locus_tag="PKH68_00930"
     CDS             complement(24116..25183)
                     /locus_tag="PKH68_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNX11527.1"
                     /translation="MNKTSKIYVAGHRGLVGSAIWNNLLSKGYTNLIGRTFEELDLMD
                     GVQVAKFFDEEKPEYVFLAAAHVGGIIANNTYRADFIYRNLQIQNNVISESYRHNVKK
                     LLFLGSTCIYPRDAPQPMAEDALLTGPLEYTNEPYAIAKIAGLKMCESFNIQYGTNYI
                     AVMPTNLYGPNDNFDLEKSHVLPAMIRKVHLAACLHKNDLEAIRRDLNKRPVEGVNGE
                     SSEGEILTILHKYGITDTGIELWGTGEPLREFLWSEEMADATVFVMEKVDFRDLSEGM
                     KEVRNCHINIGTGKEISIRNLSQLVAGVVGYNGEIRFDATKPDGTMRKLIDVTKLNTL
                     GWKHKIEIEEGVKRMYEWYCK"
     gene            complement(25357..26436)
                     /gene="gmd"
                     /locus_tag="PKH68_00935"
     CDS             complement(25357..26436)
                     /gene="gmd"
                     /locus_tag="PKH68_00935"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNX11528.1"
                     /translation="MKKVALITGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTGRI
                     EHLYFDEWVRDMHKQRTINLHYGDMTDSSSLVRIIQTVKPDEIYNLAAQSHVKVSFDV
                     PEFTAETDAVGTLRLLEAVRILGLEKSTRIYQASTSELFGLVQEVPQKETTPFYPRSP
                     YGVAKLYGYWITKNYRESYGMYAVNGILFNHESERRGETFVTRKITLAVARIAQGMQE
                     KLYMGNIDSLRDWGYARDYVECMWMILQHPVPEDFVIATGEMHTVREFCTLAFAQAGI
                     EIRWEGTGVDEKGIDVATNRVLVEIDPKYFRPSEVEQLLGDPTKARTLLGWNPTKTSF
                     PELIRIMVEHDMRFVKKLKVRGEME"
     gene            complement(26465..27805)
                     /gene="fucP"
                     /locus_tag="PKH68_00940"
     CDS             complement(26465..27805)
                     /gene="fucP"
                     /locus_tag="PKH68_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HNX11529.1"
                     /translation="MAKSLFKTASGKNYLFPFILITSLFFLWGMAHSLLDILNKHFQD
                     ALDLSKAQSGLVQASAYGAYFLMAIPAGLVARKFGYKKGVLLGLLLFAIGCFWFVPAV
                     GINKFWAFLLGLLVIFSGLTFLETVANPYTTVLGDPKYAASRINLAQTFNALGWIVGP
                     LVGSLFIFRNTSTDNMLVSFGKTLKIIFTGVADNATNVVTETGKAVENSALIIPYVSL
                     GVVVVLVFVMFFLVKLPEITSETEANIADNNPTSGIKTKRIPKPLIKQRHFVMAVVAQ
                     FLYVAAQTGIGSFFVNYTIETKNLGIGEMQAGFLLGLGGMVLFATGRFLGSFLMRYVK
                     AELLLGTFALVNTLLMFVTAIRHDNLGIIALILSYLFMSIMFPSIFALGIRGLGEQTK
                     TASSVMVMTIVGGAVCPALMGLIGEQNMNTGFIIPMICFAYIAVFGYVQSKQIH"
     assembly_gap    28070..28169
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28195..29334)
                     /locus_tag="PKH68_00945"
     CDS             complement(28195..29334)
                     /locus_tag="PKH68_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNX11530.1"
                     /translation="MPKKVFVSGSYDMLHSGHVAFFEEAASYGDLYVGLGSDKTIADL
                     KARKTVNSDAERLYMVKSLKMVKDAWINSGSGIMDFLEELKALKPDIFFVNTDGHAPA
                     KEELCKELGIEYIVSKRIPHAGLPERSTTALRQVCRIPYRIDLAGGWLDQPYVSKFHP
                     GAVITICIEPDFEFNNRSGMSTSSRNKAIELWQTDIPEGDKVKLAKTLFCFENPPGTK
                     YVSGSQDALGITLPCLNRFYYERGEYWPSKIETVTDNEILTWIEQRLWLIPLTPRVAS
                     YDVLANTDINEEKARALSVATDKCWDALLERNAVEVGKTIAQSFEAQIAMFPNMVIPE
                     ILEQLELYKNHTLGWKLSGAGGGGYLILFNEKPVENAIQIRIRRG"
BASE COUNT         8228 a         6448 c         6029 g         8435 t
ORIGIN      
        1 ccagtaccac cgaagtgtcg tttcgaagca gggttttcat ttcaaaacgg gcattggaag
       61 cagtctggag gctgatatat ccattcacgg tatcgagtgc aagtaaatgt acgttgtatc
      121 caaattgatt gcggatgctg tcgcgccctt cggatttgta gtattcagtg agttcgagcc
      181 gcatcgaact tgataacccg ggcaggataa tggcaggcat atccacaaaa tagctgtcaa
      241 tggtctgagc acgccccggg agattgaaca tcagacataa tgcgataagt aaaaatttag
      301 ttggtttcat ccgttggttt tattgtagtt gtgtcaacag ggattggtac aggtttggaa
      361 atcacacgcc agtagtgtct gatcagttca tcccaggtat tgaatgtctc tttgtacata
      421 agacccacac cctgaataaa aggtgcagaa cgtagtgtgt atcgatcgac ggtgtgatta
      481 tatgccttca gacgtagttt cccgttttca gtgagtatat attccacatc cacatcgccg
      541 ataattttat tttttgccag atcatcattc cggtaaccca ggtttccgtt taccagcagt
      601 cggttgttgg gctgatagga tatttcggcc tcgtattcct gcgctgcctg tgtgcctact
      661 ccggtgctac gtatattggc acccagctgg aatgtatttg acatttgcga aagccagtta
      721 ttgatttgcc caaatgcggt gttggcaaaa agcgacatgg tattggtacc cagcgatgtg
      781 gcaaaggtgg taactccttt ggaataatcg ggtgtataaa atttattgaa cagcaacagg
      841 tacatgatct gccggttcat catttcttcg gtgttaatca gatttcgaac ctgcattttg
      901 agtgtttcat cgctggtagg caggtcaata tcgaatttta tatccgggct catcaggtct
      961 ccagtaagtt tcagcaccgt attgaccgga acgcttgtac gcgatgtgga actcagtatg
     1021 gcgtcattca tcaaatcgcg aagtgaagca gtgagagtat aaatggcgtt gatgttcatc
     1081 tgtgcccgta gcatatcacc ggtccaggat atattgcttc cctggttgat tttaaaatcc
     1141 tttcgtatca gattttgcag cgtgaagagg taatttccct cgtcgattgt gtaggttcca
     1201 tacagcttga tatccgaata gtcgtcgtat tccattctga gactgccgct gccggtgccg
     1261 gaaagcatat caccggcctt gggatcaata atgagttgaa tatcggcatc cggtgtaaca
     1321 tctaccatca ggttcattct caggcgtgag ggtttagctt tagtctgggc gattgctgct
     1381 gattctgttt ttgttgtatc tttctgattg acaaaggtaa tgaagttgtt ttcgttggct
     1441 atggcagccc cgcccactga gataaaaacc ttggttttgg gctgtgtggt agcatttaca
     1501 tccacataac tgtttgttaa attgccgtaa atatttacat tcccggtagc ataggccttt
     1561 ccgtaaaaga attcattgtc ctttgcttcg gtattgagag caagcatatt tcgggctctg
     1621 attttcagat catatttcat gtcctgaaat gtaccgtcgt gagtgatcag tccgttaagc
     1681 gttccggtat ttcgttcttc atcatacacc gtaatatttt tcatggcaat ggacttacgt
     1741 gtgagatata ccatgtcggt gaatgaatat ttcgttttga gataatcgat ggtcatactg
     1801 gcattttcgg catacacatt accctcaaag ccgatttttt ttgatttccc aaacatgcgt
     1861 acctttccgg tgccgtatcc gttaatatct ttggctatcg aactcaggta tggacgaaga
     1921 aatccgaggt tcaacttatg tgcatcgaat atgaaatcga gtgaatctgt tttgggtata
     1981 tacacaccat tagccagtgc tacggtatca gtaccattca caaatttgcc ggaagcctga
     2041 atttcctgcc tctcgcgatc ccaggtggat agcagctgtg catcacctac gttcgagcca
     2101 tttaatcccg catttttaac gaatattttg gcatcgaata ccggcttttt tagcagtgaa
     2161 tatacaccca cctgcccggt agcatttcct ttaatggata tgtctttcag ttttacgagt
     2221 gacataataa aatcgatatt cagatcattc atatccaccg acaaaatatc attttcgttt
     2281 cgtgaaaccc gcccgttgag attgatatac tgtttccggt tctgaattct gaaattacgc
     2341 acatccatgg tggtatcggg attgatgcta agctgtgagg caagcacatc ccaggtggta
     2401 tcggcaataa taatctgagt gggcagaaat gcgatgagtg ccgaagtgag gttattctct
     2461 ttatgaaact ttgtattggc ctgaatttca cctgcataat tcaccgtatc gttgttttgc
     2521 catccaagct gggtatagag tgagtcgttg gcggctgcag cattgatatt tatgctggtt
     2581 tcactgtttt tatttatcag gacagcatta gcctgcatat tcagtttttg ttgctcattg
     2641 ttcagtgtga gagcgatatt actgattttt tgtttgccat aagataaata aggtgtttga
     2701 gctatcagtt cgacacgttc atgaatgtcg tccacatagc cttttaccgt tgttattcca
     2761 tcgagtgtga atggaagatt gaaaactttg gttaaaagtt tggtgtctga cagggtaaga
     2821 tcgatattca tgtaattgtg cgccccaccc tgcttccagg ttccgggtat atttttgccc
     2881 gaaagtgccg gaatgtaatt ttccactatc gacgaaatag tttgcggtat ggtgctgtac
     2941 ttaaaatctc cttcgaaatt tccgtttatc aggtctgagg tgatggtgaa tttagatgat
     3001 tttccactca cttccgattc aaacagcaac tgatccatca cgagttcttc accggcattg
     3061 atgaaccgga tactgtccag cagcaggtat ccgttcaggt tgttgaggga gttaccaatc
     3121 atgttcgtat tgccgtagaa cgaaaggcgc gaatctttgt attcctttgt gagatgcagg
     3181 gcgttgatat cagcattatt gacaagcaga tcgaaattga aaacgggaag ttttttggtg
     3241 agatccacta caccgtcgaa actggccgtg aggttcggat cattcacatc catctttcct
     3301 tcaaatcccg atccatcgta ctttccatcc acacggattt tttgataatt gtagcggttc
     3361 aggtaaattt ctgctatatc accgcttatt gttccctgca ggctgcggtt ttggagtttt
     3421 gttccttttg tattgatttt gaaagccgtt ttcccaaggg ttttggtgct gagcagatct
     3481 ccaagctgaa aactgctggt acgtatagtt ccgttgtatt tcagatcttt catgttgttt
     3541 tcgaactgca gcagaatatc ggtattgagc gatccggcat tggtggtgat attcccgtag
     3601 gctaccagat tgctgaagaa gcccgaaata ttaccggtat atttcaccat tccgagccgc
     3661 gacaattcct tggggagctg aaatggtttg tgagtcagct gtgaaataaa atcctgcgca
     3721 tcgcccttgt tgacatgaaa atcgcggata ttggcatata taaatgtttc gtcgaggttt
     3781 ggaaatccgt tcagatccag atcggtcttc atggtgagtg aatttccata tttcagttcc
     3841 aacttttgta atctgaagct ggatatccgg ccactgagat taccctgaat cgtggcagca
     3901 tctttcatat acctgaaatt gggaacaaat gccgacaaat ctttcagtgt gatttccgat
     3961 ggtttaatct ttgcagtcca tttcacctta ttctgtatat ctttgaggct ggcaatgctg
     4021 tcatatgtca tggaaaccga atccatcgcc agtatcgaat ttggcagcga tatattcaga
     4081 taaggcatcc gaaatccttt tttccaaccg tggatatacg tttccaaatt cctgactgta
     4141 agtcccgact gctccacagc actgaacgaa cggatgtgtg ccgacagaga gtcttttttc
     4201 agataatcca cattgagctt catgttcagt tgcctgaaat ccattcggtt ggcatcaaaa
     4261 cgggtggaat cgccagggga aggatattta agattggtta agcggaaatg cgaattgaaa
     4321 atgtttatat ttttgaaatt aaactccaca ttcgactcct tctttggttt ttttgtttgc
     4381 cgtaatgctt ttagtataaa gtcgaggttg ctcacacctg cggtatcaat caccagattg
     4441 ccatacagat ggtcaatatt tatttttttg aaaataaact tacccgaaaa gaattcccag
     4501 aaatcgaacc cggcgtcacc gctttccaca aagaggaggg tatccttctg cttatcctgg
     4561 atgtataaac tatcaaaact gaaagaattg aaaaactgat aatctacctt tccgatactc
     4621 acccgtgtac ctaatttttc cgacagttga tgggttgcct gatcggcaat ataattctgt
     4681 atcgtgttgt tttttaacgc gataaccgcc agcgaaagaa ataaaatgac gccaagcaga
     4741 aaaaatccga ttatctgaac gagttttttt atatctttga atattatttc tacaaaagta
     4801 aataaaaaat cactataata atttactcaa cacctattaa taggtacatt tactggaatt
     4861 ttatctatag attcaaaatt taggccaatg tagtttggtg tttctatcag aacaaaattc
     4921 tatctctttc gttactttat tttttaccat tacaacagaa aaattatgtc agaaaatatc
     4981 accatcatag gaattgaatc atcgtgcgac gacacatcgg ctgcagtaat ccgggatgga
     5041 gtgctgctct ccaatgtcat tgccggacaa gcggtacata cccgttacgg aggtgtggtt
     5101 ccggagctgg cttcgcgggc tcatcagcag aatatcatac cggtagtaca cgatgccatg
     5161 ctacaggcgg gcatcgacag gaaagatctc acagcaatag ctttcacacg cggtcccgga
     5221 ttgctgggtt cgctgttggt gggttcttct tttgccaagg gattgtcgca atcgctcggt
     5281 gttccgcttg tggatgtcaa tcatctgcag ggacacgtac tggctcattt tatcagtcag
     5341 ggtcatgaag gggaaaaatt tccggccttt ccatttctgt gccttttagt ttccggtggc
     5401 aattcacaaa tcgtactggt acgcgattac ctggatatgg aagtgatcgg tcaaaccatc
     5461 gatgatgcag cgggtgaagc tttcgacaaa tgtgccaaag tgatggggct tccctacccc
     5521 ggtggacctc acgtagaccg actggctaaa gaaggtaatc cgaaagcttt taaacttaac
     5581 aaaccacaga tatcgggtta cgattacagc ttcagtggtc tgaagacctc gttcctttat
     5641 ctgttgcggg atgaaatcaa aaaaaatcct gactttgttc aggaaaatac cgctgatttg
     5701 tgtgccagtc tgcaggccac aatcatagaa atactgatga aaaaactgct gaaagcgtca
     5761 gaagaactga atatcaggca ggtggcagtg gccggaggtg tttcggccaa ttcgggattg
     5821 cggaatgcat ttcacgatta tgcccaaaaa tacggctggg aaatatttat tcctcccttt
     5881 gcttttacca ccgacaatgc agccatgata gccattacgg gatattacaa atacctgcgg
     5941 ggagaattca gctgcctcga agatgtgccc tatgcccgtg taagtaattt attggtgtaa
     6001 aaaaatgaaa ctgaccctgc ccgaaaaact caaaccttac agcggagtga ttaattttat
     6061 gattattctg cttgtgtcgc atttcttctg gaaatacacc gtgctcggtg aggatgatga
     6121 tgaaattgtt cgttttttcg ggttgaatat atcccaacca tttattttta tggccagtca
     6181 cacggctcag gtaagttact cattgctgaa gtggttaggt ttcaatgtca cactgaatga
     6241 tctgaatgtc atccgccacc tgggctcagg caacggtgtg catatcgttt ggtcgtgtac
     6301 gggtatcaag caggcttaca tcctgttttg cattctcgca ttctcccgtg ggccgtggaa
     6361 acacaaactt tggtatattc cactcggctt gttatgtgtg tatctattca atatccttcg
     6421 catcactttt attaccggag tgattgatag tcatcctcag cagtttgaat tctggcacga
     6481 acatgtgaca aaatatgcat tctatgcaat gatcttcggg ttgtgggtga tttgggaaga
     6541 gaagataaga ccccaaaccc ctaaaggggc ttaaataacc caataacaga gttattgggt
     6601 tatttagtta ttaagttatt ggttattggg ttattaacaa caaaaaaacc tgacagaaaa
     6661 tatttttctt tcaggttttt tttgttcatt ctgttcttat ttttcactaa aagctataat
     6721 cgacgtatcc ttcttttacc ttaaatattt ttggtatttc accctgcaac acgcggagtg
     6781 cattggaagc cagtgcagcc atttcgtctt cgccgggata cacatacaca gttgccaatg
     6841 gatcaatcat cgccttcaca taatccacaa taaaatcgtt gtaggctacg cccccggtga
     6901 ggattacccc atccacttcg cccttcaaaa ctgccagcat tgctccaatt tccttgccca
     6961 ccgaataagc aaatgcctgt aaaaccaatg ccacgtgact gtcgcctgct tcggcttttt
     7021 tcacaagttc ctgaacgttg ttcgacccgc agtgggctat cagtccgccc tgtccggcta
     7081 atttgcgcat cagttcatgc tgcgtatatt ttcccgagaa acacatcctt accagcagcc
     7141 cggcatcgac agtacctgcc cgttcgggcg aaatagctcc ccaaccgtcg agaccctgat
     7201 tgacttcgat aacccgtccc tttcggtgtg caccaatggt tactccacct cccagatgtg
     7261 ctaccaccaa attcatgtcc tcatagttct ttcccacttt tgatgcatgc agtcgggcgg
     7321 tagctttgtg gttaagagca tgaaatatgg tacggcgcgg aagctcgggc attccgctta
     7381 tgcgggctac ctcttccagt tcgtcaacag ccacgggatc ggcaatatag gctttacaac
     7441 ctggtattcc ccgggcaatt ttatcggcca aaaatgcacc cagattactg gcatgttcgc
     7501 ccctttcatg ttttcgaaga gtttcgagca tttcttcatt cacttcgtac acacccgact
     7561 ctaccggtgg aaccatgccg ccacgcccca taatgattga gaattccgac aaatccacgc
     7621 cgttttcgtc caacgttttc attatcagct catagcggaa atcaaactga tcgagaatat
     7681 gctggtactg aattacttct tcggtactgt gacgtacggt gtaaatggcc gtctgaagat
     7741 cgtcttcata tacagcaaat ttagtggagg ttgaaccggg atttataact aatattttat
     7801 gcataattat tttttttcga caaagataaa tgaattacgg aaatagaaag aggatattgt
     7861 ctgattattt ttgaaaaaat gcaaatggtg ttataaagcg gaaacaaggt gattaaaaaa
     7921 ttaattgatt tacaactatt tcctgaatat tgtcactgaa agtgaaaacg ggacatataa
     7981 actgataaaa aaacagagga gataagaatt tattcagtcc gaaaataaaa aaaagtgacc
     8041 ggtaccaatt tcggcattat gtgctgaatg taccggtcat attattgatt ttttcttttt
     8101 ttcccttcta agatttcgct aatcagatta caatgtcatt ttctgaagcc attcctccag
     8161 ttgcctgtcg gctgaggcaa ctcttccacc ccgtatggca atgccggtaa taagttttgc
     8221 acccggacaa agtttctgca aatcctgctc gctgtagcct attccgctcc cttcgtgggt
     8281 gcagaaggga ataatcgttt tgcctgagaa atcggctgac tccagaaaag tgcatactgc
     8341 cataggaaaa gttccccacc agttggggta gccgaggtaa acaacatcgt aatcctccat
     8401 atttttcaca tattcagcca actccgggcg ggcattggta cgaagttcat ttctggctac
     8461 ccgcgtagtg tcaatatatt cctccggata ttcctgtagg gtccgtatct caaacaagtc
     8521 gccacccatg agctgctgaa tcttatgagc cagtacttct gtatttccca cctccagcgt
     8581 gaccactttt ccatccatgt agttttttcc cctgcgtgaa aaataggcta tcagtatttt
     8641 aatatctgtt acgggtaata tcattctctt cagtaggttc cttttcgtgg agcttatatt
     8701 gattataatt caatttcttc tgccaaatat atcgttttaa acataaaacc ggtacatttt
     8761 atttcccaat ctacattaaa aaagtgtaaa atactcacat acagagattg tagaagtcct
     8821 gcaaatacga tgaacagggt gatgtggaat aattgacaaa taataaatcc actcaacaaa
     8881 taattattat tttaaaaaca ccattttttt gtaaattaat aattttttta tacatttaca
     8941 gcgaaataaa tataaaaatt tagtaatatc tatttatttg attttcaatt tattacacaa
     9001 ggttaaattg ttgattttta caggaaaaag tgataatttt ctgatgaaac actatccaca
     9061 tatatttttt atctgtttta ttttactttt atccttcgga tgcagtaaaa catccaacgt
     9121 tccttatcct gaaccgttgg ccaaagccat tacctatttt tacaacgaaa atgaaaatga
     9181 gaaggtaact gacgagttga ataaacttaa tcctgagaaa tcaagtgaag aagttaatgg
     9241 gatggcacag cttttcagag ctgccgctct ctgcgaaacc gaaaaagtgg acagtgcttt
     9301 tacattgtta aaaagcataa aacctctccc caaaaacccc cggtttatgt tttggcacaa
     9361 cagcataagg gggctcattc ttttcagaca gaatgagttg ttggattccc ataaagcact
     9421 ggctgccacc accacatcaa attataagga taccagagcc cttgcattga acgaaagaat
     9481 tctggcacga atatcttttt tacttagcga tcagaccaaa ggaattgagt ggttaacagc
     9541 atcatcgcaa cattttgaag aatcaggatt aattaaaagc acgggcatta acctgaaaat
     9601 actgggcaga taccacctaa acaaccgtaa ctatgccaag gcacttcagt cattcaggtc
     9661 tgccgaaaac atattcatta agtataatga caaggcagaa cttttttata tttatattaa
     9721 tttcatggac tactgccttg taacaaagca actgaaagct gcacgatatt acgccaacaa
     9781 aagtgtatct gaaagtaatg aaattatgga taattcgatg cgtgcattag cttacaacaa
     9841 tctggcggaa atagaaatga cattggaaaa ttacgataca gcgattcatt atctgaatca
     9901 tacactggaa attccggcca actacagcac tttcaatata cgtcgagttt atgcacacat
     9961 aaaactttcg gacatttatc tgcaaaaaaa agacacctta caggcacatc aacagttatt
    10021 ggccgcacga cggtcattac ccgtttacgg ctacctggaa ttacgccatc aaacctatgc
    10081 caggctggcc aaaacctata aagctataaa taatataaag tcagcatata cctccctcga
    10141 tactgccaaa atgtatctgg acagtacttt ttattcattg actaatgaca cgaaaacctt
    10201 ttacgatacg aaaaacgaac tcacaaaatc ggcatacaat cttgagaatt ttaaaactac
    10261 taaacaaaga aacgatcaaa tcaacctgtt agtaatttta ggattaattg ttcttgctgc
    10321 ctttgtgctt atcatttacc ggcagcaacg cgaaaaaaat aaagtactga gcacgctggt
    10381 acaaaaaaac atccggttgg tggaagaaga gcgcaaactt cgacaaacac agcaagctaa
    10441 aaccaaacaa aaaaacggac gaaaaacaac tgaagtcgat aataagtatc agcaaatcta
    10501 cgaacagttg atacaatggc tggaaactga taaaaatttc agcagaaaag aactcacact
    10561 cgattcgata gctaaagaac tgaataccaa tcgcgaatat ctttcgcgtg ccatcagcga
    10621 acaaagcctg cgcttcaatg acctcatcaa caaataccgg gtggacgaag tcatttccat
    10681 actctccgac cccaccaacc acagcaacaa atataacctt gccgtgatcg gcaatcaggc
    10741 cggatttaat tcaaactccg tttttattga agcttttcgc aaacaaaccg gcatgaatcc
    10801 cgcccaattc cgcgaaaata tcgaaaatcc cgcaagggga taaaaaatat tataaccttg
    10861 atttactgct taatgcggct ttgaattggt caatccaaag aacaatattt tggatggcaa
    10921 catttattga cttacttttg ggatgaaaac aaagtgatgt tgacaccaaa aaagaaatca
    10981 acatataaaa ctttgattag taaatttaaa actgtttgtt tacatttcat cattaactta
    11041 tgtgtggttg ttgttgaaaa ggctccggtg atgtttcacc ggggcttttt ttatgtaaac
    11101 gatagagaaa aaacaaaatt gaagatgaat atttgccaaa tctgtttttc aatcgtagat
    11161 ttgtccaatt aatcaaaatt agttctcaat tcacaatctc atcgctaaca ggcaaaaccc
    11221 ctttggcagg aattttgaag aaattatatt actaaggact aaattacata tacaaacatg
    11281 aagaaaataa tccttttagc aatcacgctg ctgcttgccg gttattcagc ttattcgcag
    11341 cagacctcgg ttatcagcga aattttcagc cgctatgcac accagaaagt ggcagaaagg
    11401 caaaaactca tcctttttga cgacgctcag gctgcagagc tcgaagcgat ggaataccaa
    11461 tacctgctcg acttgcagaa agcagaaacg ggatgccgct gtcgtgcaca aaaacggacg
    11521 gaaaaactgg atagactgag agacctcaac ttacaaaaaa tccttacccg cgaacagtat
    11581 ctgaaatacg aagcactgga taaggataaa atccgcaaac tacccctccg attgactgaa
    11641 taaaaaacgg gaggtgatgt tttcacatat cctcccgttg aattctttta cagacaagtt
    11701 acctcattat attctgcatt ggtggcagcg atttttgctt caaattatca atgataaccg
    11761 cttcgcgtcg tacatcgcgc ggattgtgaa gcgttacgcc tgcttgtggt aatgctttca
    11821 cctggctgag acttggggga ggcaccggtt tgtagttaat atcgccggta atcagataga
    11881 gtgcctcgcc cagatacaca tcggccggat caccaatgtc agtccagttc cttatatagc
    11941 caaatccttc cttcaatgga taatcgggca ccagataacc cggatttgaa aaatcggcac
    12001 cgcttttatt ggtatatttt gccacaatgg gttgcatggc ccagttgccc agttttgctt
    12061 tttcggcagc agttagcgat gctgcactgt aaatgtagac accaatatcg ctgtcataag
    12121 gatgcaaggt ccatgaagcg gtatattttc caccggtgtt ttctccgata tgaaccactt
    12181 ctatatatgg cttcaggcaa aaggtggtca attccgaagc tgaataagaa tcaccggtag
    12241 caattatata aactttattc aaattcagat ttgcccccag tggatctttc tctttatcac
    12301 cgtaaacgcc caggctgtta gtacggctca ctttttcctt atcgaaagca ctattcagaa
    12361 aatcattgta cgacataatt accagtggtg atttattctg caccacggca gccggagcta
    12421 tcattgaaca cagataagat gctgctgtaa gtgcacctcc atgattatat ctcagatcga
    12481 gtaccaggtc ggtaacaccg gcctgacgga aactactgaa aacggagtag agactatcgt
    12541 tgtaattgga gataaaatca gtgtaaaaaa gataaccaat tttctttcct cctgcttcat
    12601 aaatgcgatt gtagagcacc ggattggtct gaattgtttt cggggtgaca gttacaatcc
    12661 ggtcctgaac caccgagcta cccgaaagtt tatacatcgt aaacgaggcg gcatcggtac
    12721 cgtaaagcag ggtgtagttc tcctgtgtaa ttgcatttcc gtttattttt ccaataatat
    12781 ctttccgttg caacccggca gccgatgccg gtgtgtttgg aaatacatat ttgataatgg
    12841 cgtaatattc cgtgcgggct tcattggcag ctgcaaaagt cagggagaat ccgaaagcct
    12901 ttggttcacc ggcaaaatcg gccaataaac tctgtacatc gtcagtaata aatgaccagc
    12961 catgatcctt gtcggtggca ttcagcagac tttcaaaata ttccaccgga tcggtatctg
    13021 ccacagtggg ttttttattc ttcatttcgt cggcccagag ataataggtc gataatccgt
    13081 cataaacaaa ctgcgaaaca agttttgaat catcagctac cgggtctttg ggtttacagg
    13141 aactctgaat taacagaagc ggaaggagaa aaagaatacc aatttttttt atcataatgt
    13201 tacgtattgt tttatcattg attttcaacg tgcgaaatta caaaagaatc ggagctatgc
    13261 acttagttta ggatttttta agtttagtaa tttctttcgg attttttgtt tgatctttgt
    13321 tatcaaacca ttcatcggga aaattaacca gaaagacctg ccccgaagcg cgggtaagtg
    13381 cggtgtaaaa ccaccggaaa tactcgcggt taatctgatc gtcgggcagg taaccctgat
    13441 cgataaatac cttatcccac tgaccgccct gagctttgtg acaggttaca gcataagcaa
    13501 atttcacttg cagggcgttg taatattcat tatcgagaat tttcttaaac aacagtttcc
    13561 ggttcgttat ttcggggtaa tcctcggcaa ttgcctcaaa aagcttcccg gtcatttcat
    13621 tcatccgggc cggactttcg gcctgcaggc tgtccatcca tatccgcgca tcaatttccc
    13681 agccgtaatc gagcgaccgc agactcaaat ccacaaagcg aaatccgtat atttcacggt
    13741 gtttgccaat gcggataact tccagcacat caccgttggc tataaattca atttctttgt
    13801 agggtttatt ccaaaaataa ttatttcggg taaccatcag cagatcaccg ttggagagtt
    13861 cctcttcctt catcatcacc cgcgaccgta tgccgttgtt gtacatatta gcccgtttat
    13921 tcgagcgggt tatcacaatg gtattctcca cccccactcc attgtatgcc cgctgaattt
    13981 catctatcag atccattccg tttaagcggc ttatgtccgt aaaatctctc agttcaaatt
    14041 ttggatgatg agaaatatta tcttctgcca aatccagcct cagctgtgtg gcatgataca
    14101 atatgccgct ttcgagtgcc tgccgaacca cgtgtgtgag ggtaaattca tagactttca
    14161 gtccgtaggc ttgaagcgca ccggtttcga gggccggact ttgcggctgc tccaccggag
    14221 gaagctgtgc cgtatcaccc agcagcaaca gactgcattc gtcaccgccg tagacatatt
    14281 ctatcaggtc gtcgagcaac cgcccgctcc cgaaagaccc gtctgttccg ttgttggaaa
    14341 tcatggaagc ctcatcaaca atgaagagcg tgtgtttatg cagattgtcg gccaactgaa
    14401 atcggaattc ggacatcgat ttctgacggt aaatcttttt atggatggtg aaagccggga
    14461 atcccgaata accggaaagc acctttgcgg cacgcccggt gggtgcgaga agtactgttt
    14521 tttgctccaa aatattcagt gccctgacca gcgcacttac cactgaagtt ttaccggttc
    14581 ccgcataacc tttgagcagg aacagttttt cgggttcagg ggacataaca aattcgccca
    14641 accggtcaat cagttgttcc tgctgctcag tgggttcaaa aggtaattcg gtacgtattc
    14701 gctcgctgat aaaattctgt aacatatcca caaaggtaat gaatctgcta aagcagtcaa
    14761 acagaaaaag ggtttttaat ttcacaaaag aattatttcg tatgcatccg gtagattatt
    14821 tttaagaatc atagatgttt cttttttaca ctgtccgcgt tttctgcttt tcatccactg
    14881 agtttcgcaa ttttttgtaa ctttgtatgt tctattacaa ttaattctaa gtttcacttc
    14941 ataaaagcat taatcaatga aaaaaataat tttatacctt atggttccaa ttttatttgc
    15001 ctgtgacggc aaaaaaactg aaaatgtcac tccatctgtt gagaatttca actactcagt
    15061 tgacaaattt gctgatgttc agatacttcg ttatcgcgta ccggatttcg aaaaactgac
    15121 actgaaacag aaagagatga tttattattt atcacaggcg gccatcgaag gacgcgatat
    15181 cctgtacgat cagaacaaca aacataatct gagtatacgc cgcacactgg aagccgtgta
    15241 tgaaaattat aaaggtgacc gcaacgctga agcgttcaca caactcacca tctatctgaa
    15301 gcgtgtgtgg atggccaacg gaattcatca tcattattcc gaagataaat tcacacctga
    15361 attcagtgca gcctattttg ctgaaacagt acgaagcatt gatccggcaa aactaccgtt
    15421 gcagcagggc gaatcggttg atcagttaat tgctaaactt agcccggtaa tattcgaccc
    15481 caccgtttat cccaagcgta ccaatcaggc agatggcgtg gatttgattc tcacttcggc
    15541 caataactac tacgaagggg ttacccaaca ggaagcagag gatttttatg ccaacatgaa
    15601 aaatcccaac gacagcacac ccatttcgta tggattgaac agcaaactgg tgaaagaaaa
    15661 cggaaaaata gtggaaaaaa cctacaaaat tggcggaatg tattcagaag cgctgagccg
    15721 tgtggcaggg tggctcgaaa aagcagcagc cgtggctgaa aatgatcaac agcgggatat
    15781 tattaataca ctcatcagtt tcaaccgcac cggtgacctg aaaacttttg acgaatactg
    15841 tatcaaatgg gttcaggacc tgacttctca ggttgatttt gtcaacggat ttaccgaaac
    15901 ttatgccgac cccctgggaa tgaaagccag ttgggaatcc attgtcaact tcaagaacat
    15961 cgaagcaacc aaacgcaccg aaatcatcag cagcaatgcc cagtggtttg aggacaattc
    16021 tcccgtcgat aaaaatttca agaaagaaaa ggtaaaagga gtttcagcta aagtaattac
    16081 cgcagccatg cttggaggtg attgctaccc tcccacaccc attggcatca acctgcccaa
    16141 tgccaactgg atacgcgctg aatacggttc gaaatctgta actatcgaaa atatcaccga
    16201 agcttacgat caggtagcta tgggcaatgg tttcaacgag gaatttatgt ggtcggacgt
    16261 tgaacgcaac cgggccaggg aatatgcttc actaaccaat aacctgcata ccgacctgca
    16321 cgaatgtctg ggacacggat ctggcaaact cctgccgggc actgatccca acgcgctgaa
    16381 agcatacgac tccaccatcg aagaagcacg cgccgatttg tttggccttt attacatggg
    16441 cgatcctaaa ttggttgaac ttggtttatt acccgacaag gaagctttca aggctgaata
    16501 ctaccaattc ctgatgaatg gactgatgac ccaactcacc cgcatcaaac ccggaaaaaa
    16561 cattgaagaa gcacacatgc gcaaccgtca actgatagcc aactgggtgt acgaaaacgg
    16621 gaaagccgac aatgtggtgg aattcaaagt tcgtgatggt gaaacatttg tggtggttaa
    16681 tgattatgat aaagtacgca ccctgtttgg caaactgctg gctgaaattc aacgcgtaaa
    16741 atcaaccgga gattttgccg gtggacggga tttggtggaa aaatatgccg taaaagtaaa
    16801 tcccgaacta cacaaagaag tgcttgttcg ctatgaaaag ctcaacctgg ctccgtacaa
    16861 aggctttgtt aatcctgttt acgcacttgt taccgataaa aaaggaaaag ttaaagatgt
    16921 aacagtttca tacaccgaaa attacgtcga tcagaatctg cgttattcca gggagtattc
    16981 taaattgccc aactacaact aactgatgaa ataactaata atgaaacaca tgtccaagta
    17041 aaatattttt aatacacacg ggcatggtta aaaaactcac aacaaatgat agacgctctt
    17101 tattcctccg tcagctgacg gatttggggg cagatttata aattataaac agatgaagaa
    17161 aaatttaata ttgctgatga tagtggcagg tatcaccgcc tgtattcata agagtgaaaa
    17221 cgcaggcgaa caggccgcta cagactcggt ggctgtaacc acccaagttt catccgggca
    17281 gtcacagtca acatggctgg gcgaatatac aggcattttc ccctgcggcg actgcgaagg
    17341 tattgagatt ctgatcacac tcaatacaga cagtacgtac acacagaaat caacttacct
    17401 gggaaaaggg aatagttttg aagaaacagg cacatttatg gttcaggaac ccggttcgcg
    17461 ccttgtattg aaatcatccg acagcgagca ggcttatttg ctgggcgatg gaacgcttac
    17521 cctgctcaac tccgaaggaa aagtgaccga aggcgaactt gctgaaatgt atgtactaaa
    17581 gaaaaaagag tagttatttc aaaaatatca acaaatacgg ttattagttc aggataatag
    17641 ttttaataaa tcatccatat caataattac gagccatgaa gaaccttatc atcacattga
    17701 atatcaaaaa gcattttcgg ggaatacttt tagtgttatt tcctttttta attttcttat
    17761 ttttcttacc cgttaatctc ccggcacaga cgcccgttga ctatgccatt cagggcatac
    17821 catttaatac ggtaagtctg aatgacgggt tttggttacc aaaaattgag acaaaccgca
    17881 ccatcaccat tccctcttcc ttccagaaat gtgaggaaac cggccgggtg gagaatttca
    17941 tactggcatc gggtgcaaaa aaaggaaaat ttctgactgt atatcctttc gacgataccg
    18001 atatttataa aacaattgaa ggtgcggctt tctccatgac ggtacatccc gataaagcac
    18061 tcgataggta tgttgacagt ctgatagcaa tagttgcagc tgcacaggag cccgacggat
    18121 acctctatac cgcccgcacc atcgacccgg cccatccgca cgactggtcg ggcagcgaac
    18181 gctgggtaaa agaatcggaa ctgagccacg agttatataa ttccggtcac ctgttcgagg
    18241 cagccgctgc tcactttata gctaccggaa aacgtaattt actaaatatt gcactgaaaa
    18301 atgccaatct gctggtgcag gttttcggtc ccggaaaacg tgatgatgct ccgggtcatg
    18361 aaattgtaga aatggggctg gtgcggttgt accggattac cggtgagaaa aaatacctgg
    18421 atctggctaa atttttcatt gattgccgtg gaaaaatccc caattcgaag caattctatt
    18481 cgcaaaacca cattccgttt gtacagcaaa ccgaagcggt tggtcatgca gtgcgggctg
    18541 catacctgta ttcgggcgtc gccgatgttg cagcacttac gggcaataag gaatatatcg
    18601 atgcaattga caaaatctgg gaaaataccg taaccaaaaa gttttatatc accggaggta
    18661 tcggagcgct tcatgcaggc gaagctttcg gggctgacta tgaattgccc aatctgacag
    18721 cctataatga aacctgtgct gccattgcca atgtgtactg gaattaccgg atgtttctgc
    18781 ttcacggaga tgccaaatac atcgatgtac tcgaacgaag tctgtacaat aatgtaattt
    18841 cgggaattgg gctggatggt aaaacatttt tttatcctaa cccgctggaa agcaatgcaa
    18901 aatatgcttt taattatggc aacaaacttt cgcgtgaacc ctggttcgat tgttcgtgct
    18961 gccccaccaa tctgtgccgc tttatggcct ctgttccggg atatatttat gctcaaaaac
    19021 aggaagatat ctacgtaaat ctgtttatcg gcagtaaaac cacgtttaaa ttacagaaaa
    19081 ccagcgttgt attaagccaa caatcaaatt atccctggga aggaaatgtg tctattcagg
    19141 tcaatccaac aaaaaagacg gatttttcac tttgcttacg aataccggga tgggctcgaa
    19201 accaaccggt tccgggtgat ctgtaccggt atgtggcaag tactcctgaa gaaatcgttg
    19261 tcaaagttaa cggaaaagca gtggcttaca caacccgaga gggctatgcc gtcatttcac
    19321 gccaatggaa aaaaggcgac aaggtggaat acacccttcc gatgaatata caccgcgtgg
    19381 aagccaacga aaaagtgaag gacgaccggg gaaagattgc catcgagcgc ggaccggtgg
    19441 tttattgcct cgaaggtatc gataataata acaatctgaa tacaatcatc ctgcccgata
    19501 atgcaccctt gtcggtaaaa tttcagcctg caaaactcaa cggaattaat gaaatttccg
    19561 gtgaagcaat tgttttggaa atagcaacgg acggactttc ggtacaaaca aaaaagcaac
    19621 cgtttaccgc cataccttat tatgcatgga gtaaccgggg tattaatcaa atgaaagtct
    19681 ggttaccgag gaaggtgaag gagataacca taagtaatga atagttagag acgaggtttt
    19741 aagtctgatt tttttgcatt aattaagtcg ggctttgatt ttctaagtat gtttcttcca
    19801 gaagtttcag gaagtcgtcg acccgttgcg ccggattaat atcattgtcg gtgttgaaag
    19861 tccagtcagc cagtttcatt ttttcttcat cgggccactg gttgcgaatt ctggtcaata
    19921 ccagttcctc tgtaacctta tcgcggtgca tcacccgttg aattcgcaca tctaccggtg
    19981 ctgttatcag caccgtttta tctaccagtt cgttaaagct gccttcaaaa agaatggcac
    20041 attccataaa cagaaatcgt tcccgggaat gctccatcgc ccattcccgg aattttttct
    20101 tcacttcggg atgaactatc tggtttagct tttgaagttt ttctgcattg ccaaatacta
    20161 tctgagccag tttcacacgg tccaaaccat tggcattgta aacttcgtca ccaaattcag
    20221 caatcacctt ttcacgcacc aggggacaaa cattctgcag gtttttcgct tccatatcgg
    20281 tatcgaaaac gagccatccc tgttcgcgta aatgacggga aaacgtggat ttaccaccac
    20341 ctatgccacc ggttatgcca atgattaacg gagttttcat caaaattcag atatttaacg
    20401 attataacgt cttttctgta cgtaatgttt gaaattgtaa taaaaaaaca gggaaattac
    20461 tgtcagaacc caaagctaaa tcctacggtc agagtggtat tttttccccg gaataaagtc
    20521 ggcgtataat aattcaacgc ttcagtagct gtcattctcc gttcaccggc aatgggatct
    20581 gaagttgcat caaaaccctc aatgttgctg ttttcaagat ttatcacagc atatccgtta
    20641 ttgaacagtt catatacaag tttcagtcca atggtttgat ttttccagat tacatcaccc
    20701 aacgaaggct gactgatgat tttgccgata gtagccactc cattttgccg gcggaggtaa
    20761 tcatattcgt taccgtgttt cgcattcagg tagctgaggc tgatatccag tccacgtaac
    20821 ggcttatacc cggccgaaag aaaaatttcc tgcgcattat cgcccaggta atgacccaga
    20881 ttatatccgt tggaagccca ggtcaataca tcgatggaat gtttgtagtt aatgatatta
    20941 gttcgggtgt actcggctgt caaactcaga ttatcaacga actggttatt catttttgct
    21001 ccaattttaa ccgaaagtgg gttcttttcg gcactcgatg gtttgaagcg gctgaatttc
    21061 atttcatcga taaacaccga ggaatacagg tgcagatgcc tgatatttcg ggaactgatg
    21121 ttgaaaaaca actgggaatt ctggttctca gttgcaatac ctttggtcat ggtgtggtca
    21181 atagacttgt agaaagccac gggaagcaga taaccgggct gcacattgtt ttcggcataa
    21241 ataatggcgt tgccaaacga gaaatgaaaa ttccggaacg gagtgagcgt aaacatattg
    21301 gcagcaataa acttattgtg attgcggtac cattttttta caccgttttc gaggtagtaa
    21361 cggctgctgt ccgctacatt ggagacgagc caaccgtgga tatagttcat ttcaaaccac
    21421 tcagcaggtt tgagacttaa actaatcatg ggaaaggaag gtgcccgacc cgaaaggata
    21481 tttgcaccgt tgtagttatc gccccacatg atgttatctt ttatcagtcc taccgatccc
    21541 catttccatc cgtaggttat tcccccgcgt gaatcgctga aatcgctggg ttctttgtat
    21601 tcatagccgg gaaaattcat cagatattcc ggttccgaca atcttccgcc tccaaatgaa
    21661 atatctctca gactaccgta aacgctgagg ttttttccga tcatgctttg aaaatcgacc
    21721 ccaaaccatc gttgcgttat tcgtccgttt ttattggaat aaatattcat acccagtatg
    21781 ggctgtatgc gggctcgaaa catgctgtcg cgataattga atatgggcgg ataaaaatca
    21841 agccgaaagt cttctttctc catcgccgga aattcaaatt cgggcaatct atcctgttcg
    21901 ggagcataat cttcgagaaa aaaatagagt tcctgtttct ggcggctgtt cagtttatct
    21961 tcctgttggc gtgcttccag cagttttcgg cagataaaat cacgtgaata tggcttgata
    22021 gccgagttca gatcaatgaa accatcggta gccagttcat ctacaaaatc gtaaatgcgg
    22081 gtgtaggtga tatgctgtgg aatatactgt gcgaatgcag caaccgaaat gagaagtatg
    22141 aaaagcgtaa agataaattt tttcatcagc tggttttatt taatcatttt tcctgttatg
    22201 gatgatcgct ttttatgccg atcgggcttt attaaacaga atgtttttca atattaagaa
    22261 gaagtatttg aaatcagttc ttaattcgcc gttcgtttca cattgtttga ggtatcgcag
    22321 ttccgaggcc tgaatttcat ccagtgtttt tggcatatcg gcataaaacg gaggtaacaa
    22381 acccggtcga taatttgttc ttttttgctg cagttcgggc gtatacagat tgaaatactg
    22441 tttgctcaat ggcctcactc ccaccagttt catatcgcct tttatcagat taatcagcat
    22501 ggggagctca tcaatccagt atttgcgcat gaaccggccc atgcttgtca cacggatatc
    22561 gcgtttgaac tttcctccct cctggaggtc gttattctca tatacatatt gctgcagaaa
    22621 ttcggcgtaa gggtgcatgg tgcgaaattt atacacactg aatgttttac cgtttttgcc
    22681 ggttcgtttg agcttgatga gcggtccgta gttgcggtcg agcaacggag ccgcattttt
    22741 cactcttcgt gccaccacaa agttcatgtc gttgattttg gattgccgct cgagcataaa
    22801 tccgcagtaa ttcaaccttc ccaatacttc ggtttttgac aatacccgtt ttttgccttc
    22861 ggtaaggtcg taatacaacc gcttggtcag aaagaatttt ggcatcaccc tgtgatacag
    22921 aaaatagaaa atataaaccg taagggcaac aggacgcggg tatttatcaa atatcctttg
    22981 tttgatggta ctttgagatt tgtagcagca cacaagtatt ccattgtcag gcagtttttc
    23041 gttgaccaac gccagcattt tgttaatgcc gcggatatga ttcagtcgct tcaactgaat
    23101 gtacgttgaa tactgataat gctcaatatc ctcgagttct ttttcattaa attcggtaaa
    23161 aatataggtt ccgctctcgt acaacattgt attcttctga agatagttaa tcactttctc
    23221 tcccgaaaat tcagttatcc gcttgtatct ttcggttacg gcatcgtcac tcatcgggtg
    23281 tgatgggtgt ggaacagccg ataatcgttt gtcgggatta agattaggag tatcatacgt
    23341 tacggcgtac cggtacagat aataagcaaa aaggaacaga tacaaactca caaaaacgcc
    23401 catcagaact gagattaaca cattctgcga ataaggactt gcgggttgga tgagaataaa
    23461 actgccaaaa atcaccagaa taatgactga ggtaaaaaaa agtgaaaagg ccgattggat
    23521 gaatgatttg gatttaatgc tattgtannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23641 nnnnnnnata tggtccgtct ttcacttcga gaataactgt tcccgactca agcacctcta
    23701 cagaatgcca ttgcccggca ggaatatcca gcccgtagtt accggttgct gtgtcaaggt
    23761 gcattgtttc gagcacttcc ttcttatcgt tgtaaagtat aacatttaat tttccgcgca
    23821 gcaacaggta tgtctcgttg gtatttggat gccgatgaat ggggagtatt gtacccggtt
    23881 cgagtgcatt gagcagcttc tgagcttttg catccagatt atcgtgcaga ttgtaattca
    23941 tccgcagccg gggactctcc ttcgcctgac gggtaatctc gtcaagaagt tctttattga
    24001 taattttcat acctgtgtta tagtcttttt tttacttcat atacttgaca aacttgatga
    24061 acttgataaa cttgatgaac ttgacaaact tgataaactt gataaacttg ataaactact
    24121 tacaatacca ctcatacatc cgtttcacac cctcttctat ttcaatctta tgcttccaac
    24181 cgagtgtatt cagtttggtt acatctatca gtttacgcat tgttccatcg ggcttggtgg
    24241 catcaaagcg gatttcgccg ttataaccta caactccggc caccagctgc gaaagattac
    24301 ggatgcttat ttctttaccg gtcccgatat tgatgtggca atttctcact tctttcattc
    24361 cttcactcaa atcgcggaaa tcaacttttt ccatgacaaa aacagttgca tctgccattt
    24421 cttcgctcca gagaaattca cgcagcggct caccggttcc ccacagttca ataccggtat
    24481 ctgtaatgcc atatttatgg agtatagtca gaatttcccc ttcactgctt tcgccgttca
    24541 ctccttccac cggtctctta ttcagatcgc gacgaatagc ttccaggtca tttttgtgca
    24601 gacaggctgc caaatgcact ttgcgtatca tggcaggtag tacgtgactt ttctccagat
    24661 caaaattatc attgggacca tacaaattag tgggcatcac tgcaatgtag ttggttccat
    24721 actggatatt gaaactttcg cacattttca gaccggcaat tttggcaatg gcataaggct
    24781 cattggtata ttccagcgga ccggtaagca gtgcatcttc ggccatcggc tgcggtgcat
    24841 cacggggata aatacaggtg ctccccaaaa acaggagttt ctttacattg tggcggtaac
    24901 tttcagagat gacattattc tgaatttgaa gatttcggta aataaaatcg gcccggtagg
    24961 tattgttggc tatgattcca cctacatgcg cggcagccaa aaaaacatat tcgggctttt
    25021 cctcatcaaa gaatttagcc acctgcacac cgtccatcag atccagttct tcgaatgtac
    25081 gaccaataag attggtgtaa cctttactca ataaattatt ccagatagcc gagcccacca
    25141 gtccgcggtg tccggcaacg tatatttttg aagttttgtt cattggaaag cccccaagcc
    25201 ccctaaaggg ggtgttatgt gcattaattg ttttttattg atttcggatg ttataatttc
    25261 cgttttctcc taatattatt ttgttattta tgaccctcat tattccccct ttagagcctg
    25321 tcccgaactt gtttcgggag ggttaggggg gccgctctat tccatctccc ctcttacctt
    25381 caactttttc acaaaccgca tatcatgttc caccataatc cggatcaatt ccgggaacga
    25441 agttttagtt gggttccagc cgagcaacgt gcgtgctttg gtgggatctc ccagcaattg
    25501 ttccacttcc gaaggacgga aatacttcgg gtcaatttct accaataccc gattggtagc
    25561 cacatcaatg cccttttcat ccacgcctgt tccttcccac ctgatttcaa ttcctgcctg
    25621 agcaaaagca agggtacaga attcgcgtac ggtgtgcatt tcgccggtgg caatcacaaa
    25681 atcttcgggt accggatgct gcagaatcat ccacatacat tccacataat cgcgggcata
    25741 accccagtca cgcagcgaat cgatattgcc catatacaat ttttcctgca ttccctgagc
    25801 aatgcgtgct actgccaatg taattttacg ggttacgaaa gtttcacccc ttcgttcgct
    25861 ttcatgattg aaaagtatgc cgttaacggc gtacatgccg tatgactcgc ggtagttttt
    25921 tgtaatccag taaccataga gtttggccac accatacgga ctgcgcggat aaaacggtgt
    25981 ggtttctttc tgaggtacct cctgcaccag cccgaacagt tcggaggtgg atgcctggta
    26041 gatgcgggta cttttttcta atccgagaat tcgcaccgct tccagtaatc ggagtgttcc
    26101 cacggcatca gtttcggctg taaattcagg tacatcgaag ctcactttta cgtgactttg
    26161 agctgccagg ttgtatattt cgtccggttt cacggtttga atgatgcgaa ccagtgaact
    26221 ggagtcggtc atatcgccgt aatgaaggtt gatggtgcgc tgtttgtgca tatcgcgcac
    26281 ccattcatca aaatacagat gttcgatacg gccagtattg aatgatgatg aacgacgcag
    26341 tataccatgc acttcgtacc ctttttcgat taaaaactct gccagaaaag aaccatcctg
    26401 accggttatc ccggttatta atgctacttt tttcataatt tgttatcttt tttatttttc
    26461 actcttaatg tatttgtttt gactgaacat aaccgaaaac ggcgatgtat gcaaaacaaa
    26521 tcatgggtat aataaatccg gtattcatat tttgttcgcc tatcaatccc atcagtgccg
    26581 gacaaactgc ccctcccact atcgtcatca ccatcaccga tgaagctgtt ttggtttgct
    26641 cgcccagtcc tctaataccc agtgcaaata ttgacggaaa catgatggac ataaataaat
    26701 aagacaaaat cagggcaata attcccagat tatcatgtcg tatagccgtt acgaacatca
    26761 acagggtgtt tactaaggca aaagtgccca gcagtaattc tgctttcaca taacgcataa
    26821 ggaaactacc aaggaaacgg ccagtggcaa acaataccat accaccaagt cccagcaaaa
    26881 atccagcctg catttcaccg atacccagat ttttagtttc tatggtataa ttcacaaaga
    26941 aactgccaat acccgtttga gccgcaacat ataaaaactg agccaccaca gccatcacaa
    27001 agtggcgttg tttaatcaac ggtttaggta tccgttttgt ttttattccc gatgtcgggt
    27061 tattatctgc aatatttgct tctgtttcgg aggttatttc aggcagtttt accagaaaaa
    27121 acatcacgaa caccagaacc accactactc caaggcttac gtaaggaatt attaatgctg
    27181 aattttctac ggcctttccg gtttcggtca ctacattcgt tgcgttatca gcaactccgg
    27241 taaagattat tttcaacgtt tttccgaacg agaccagcat attatcggtg gaagtatttc
    27301 ggaaaataaa caaactcccc accagcggcc ctacgatcca tccgagggca ttaaatgttt
    27361 gtgccagatt aatacggctg gcggcatatt ttgggtcacc gagtacagtg gtataaggat
    27421 ttgcaacagt ttccagaaaa gtcagaccgc tgaaaataac cagcaatcca agtaaaaaag
    27481 cccagaattt attaataccc acggccggaa caaaccagaa acaaccgata gcaaacaaca
    27541 gtaagcccaa cagcacaccc tttttgtacc caaatttgcg ggcaactaac ccggccggaa
    27601 tagccatcag aaaataggcg ccataggccg atgcctgcac caatcctgac tgggccttgc
    27661 tcaaatccaa cgcatcctga aaatgcttat tcagaatatc cagcagtgaa tgagccattc
    27721 cccacagaaa aaacaacgaa gtaatcagta taaacgggaa aagataattt tttccggatg
    27781 cagttttaaa taaggatttt gccatatgca caattttcta tttattaaat atttattctg
    27841 tttttcacct ctgagtagtc agcagtgagc ggtgagcggt gagctttcag cagtgagcag
    27901 tgagcagtga gcagcgagcg gtgagctttc agccaataac caatcaaaca tgcttcgact
    27961 atgctcagca accaattacc aacaccccta atacaaaggc gtctttaagt ctccccttcg
    28021 gggagattta gaggggctcc taccccctcc ttatccttat ctggattgcn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cttcggggag atttagaggg gctcctaccc
    28201 cctccttatc cttatctgga ttgcattttc aaccggtttt tcgttgaata atatcagata
    28261 gccgccacca cctgcaccac ttaacttcca acccagagta tgatttttat acaattccaa
    28321 ttgctccaga atttcgggta ttaccatatt agggaacata gctatctgag cttcaaagct
    28381 ctgcgcaatg gtttttccaa cctcaacagc atttctttcc aataaggcat cccagcattt
    28441 gtcagtagcc acactgagtg cacgggcttt ttcttcatta atatccgtat tggccagcac
    28501 atcatagctt gccacacggg gtgtaagcgg tatcagccac aaccgttgtt ctatccaggt
    28561 cagtatttcg ttatccgtta cggtttcaat tttcgaaggc cagtattccc cccgttcgta
    28621 ataaaatcga ttaagacacg gcaatgttat ccccaatgca tcctgcgatc cgcttacata
    28681 tttggttccg ggaggatttt caaaacagaa aagagttttg gccagtttaa ctttgtcacc
    28741 ttcgggtatg tcggtctgcc agagttcaat ggctttattg cggctgctgg ttgacattcc
    28801 gctgcggtta ttgaattcaa aatcgggttc aatacaaatg gtaatcactg ctccgggatg
    28861 aaatttgctt acatagggct gatcaagcca gccaccggcc aggtctatgc gataaggtat
    28921 ccggcaaacc tgacgcaacg ctgtagtgga tcgctcgggt aaaccagcgt gtggaattcg
    28981 cttactcaca atatattcaa tacccagttc cttacacagt tcctctttgg cgggtgcatg
    29041 accatcagta ttcacaaaga aaatatccgg tttcagtgcc ttcagttctt ccagaaaatc
    29101 cattatacca ctacccgaat ttatccaggc atctttcacc attttcagcg acttcaccat
    29161 atacagtcgc tcagcatccg aattcaccgt tttacgtgct ttcagatcgg caatagtttt
    29221 atccgacccc aaccccacat acaaatcacc atacgatgca gcctcctcaa aaaacgccac
    29281 gtgcccgctg tggagcatat cataacttcc ggaaacaaaa actttttttg gcataaatat
//
LOCUS       DAOPZK010000008        29114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1595_length_29114_cov_24.624247, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000008 DAOPZK010000000
VERSION     DAOPZK010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29114
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_29114_cov_24.624247"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKH68_00950"
     CDS             complement(<1..446)
                     /locus_tag="PKH68_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HNX11531.1"
                     /translation="MQNKQEINNFHSSTPTFPLVLDAAGSWGANIKPLLDEAVIRYNH
                     IDFIENDPIQIPHRFTRKEDIEIAGFLAASISWGQRKTIINNAKRLMDLMDNSPYDFI
                     MEGNDFGKLSSFIHRTFNGADCEFFLRSLRNIYLHHGGLERVFTDG"
     gene            complement(507..1094)
                     /gene="pnuC"
                     /locus_tag="PKH68_00955"
     CDS             complement(507..1094)
                     /gene="pnuC"
                     /locus_tag="PKH68_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNX11532.1"
                     /translation="MNYPELIAVVFGIASVWFARKENILVFPMGIISVLIFIYLFFTG
                     RMYANAGINLIYFFTNAFGWYNWSRKKNEEHVVTITRNTSRQNWLIIGFAIVFYVAVL
                     LLLRWYNRADAAYLHSSMPWMDALNSSLFLCATILMTIKKLENWHFWIAGNVVSIPIF
                     LIQGFYFTSFQYAVFLVLALSGLSLWRKKAKTPNP"
     gene            complement(1091..4072)
                     /locus_tag="PKH68_00960"
     CDS             complement(1091..4072)
                     /locus_tag="PKH68_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNX11533.1"
                     /translation="MNTNSIKKLYFKDTSFASLMRHRIYNILLYASKYDAFMLEEDGR
                     VDEQIFNEYVSLNLRYPPRFTLVANEEEANLALKDRTYELIISMPSGDSINPFSWAKE
                     VKITHPDIPIVVLTPFSRMVSQRIANEDLSAIDYVFSWLGNSDLLLAIVKLIEDAMNV
                     EEDVESVGVQVILFVEDSVRFYSSILPHLYKYVFVQSRSFMTEALNAHEQMLRMRGRP
                     KILLARNYEEAMQIYTKYKRNMLGVITDVNYKKNGVKNSGAGIELCREIRKQDTFIPL
                     IVQSTNENNHKLADEVKAAFVNKMSKTQMMELREIITNNFGFGDFVFANPVTGEEEAR
                     IENLRGLQDIIFKISDDTLFYHVSRNNISRWLYSRAMFPLGQFLENIHVDVQEKTDMN
                     KVRQIIFDAIVTYRRIKNRGIVAVFQRDRFDQYSNFARIGEGSMGGKGRGLAFLDAMI
                     KTNESLENFRDTQICIPRTVVLCTDNFTEFMEMNELYPIALSDVDDEVILQHFLKAKL
                     PRRIIADLYAFLGAISSPVAVRSSSLLEDSHYQPFAGIYSTYMVPYDGKSKTNMLRMI
                     TEAVKAVYASVFYRSSKAYMTATKNVIDEEKMAIVLQETCGSYHGERFYPSFSGVARS
                     LNFYPLGAEKAEEGIVNIAMGLGKYIVDGGVTLRFSPYHPNNVLQTSTMDMALRETQT
                     YFNALDAREKNFVPEVDDGFNLLKVRVQEADKDGTLKYMASTFNPRDQVIYDSVFEEG
                     RRVMTFANILQHNVFPLPKIMQEVLRIAHAEMGRPIEIEFAVTLDYSPKPTHTFYLLQ
                     IRPIVDSKEMISEDIGAIQEADTIISCENAMGHGITNDIYDIVYVKPEAFNASKNQLI
                     MYDIEKLNKKMLQQERYYVLVGPGRWGSSDVWLGIPVKWPHISNARVIVESGLSSYRI
                     DPSQGTHFFQNLTSFGVSYFTINPFQNDGSYDIDFLNAQPAEFESEYIRHVHFEHPIV
                     VKVDGRKNKGVVMKPNKEEKE"
     gene            4504..5838
                     /gene="gdhA"
                     /locus_tag="PKH68_00965"
     CDS             4504..5838
                     /gene="gdhA"
                     /locus_tag="PKH68_00965"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNX11534.1"
                     /translation="MILEKLMQQLEAKHPGEQEYLQAVREVLSTIEEVYNEHPEFEKA
                     KIAERLVEPDRIFTFKVPWVDDRGEVQLNIGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNALTTLPMGGGKGGSDFNPRGKSDAEVMRFCQSFMLELWRHIGADTDV
                     PAGDIGVGGREIGYMYGMYRKLAQMNTGVLTGKNLEFGGSLIRPEATGFGALYFVKQM
                     LETAGQDIKGKTVAVSGFGNVAWGAILKATEMGAKVVTISGPDGYIYDENGITGKKID
                     YLLELRATCNDVVAPYAEEFGAVFVPNRKPWEVKVDIALPCATQNELNGDDARKIIEN
                     GTFCVAEVSNMGCTPEAIELLIEHKIMYGPGKAVNAGGVATSGLEMTQNAMHISWGAP
                     EVDARLQQIMHDIHGQCVKYGRQPDGYINYMKGANVAGFLKVAKAMLAQGIV"
     gene            6120..6605
                     /locus_tag="PKH68_00970"
     CDS             6120..6605
                     /locus_tag="PKH68_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNX11535.1"
                     /translation="MKPNFYQFSATSLQGKEIDMKSYEGKIVLVVNTASKCGLTPQYE
                     GLEKLHKEYKDKGLVILGFPCNQFANQEPGTAKDISEGCLINYGVSFQMMSKIDVNGP
                     TAHPIYKYLKKELKGFPGSEIKWNFGKFLIDKDGNPYKRFSPLTKPEKLRSDIEKLLA
                     K"
     assembly_gap    6749..6848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6849..7277
                     /locus_tag="PKH68_00975"
     CDS             <6849..7277
                     /locus_tag="PKH68_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX11536.1"
                     /translation="NQLCFPFYAVSRLITRAYQDDLDALGITYPQYLVMMVMWEHEEL
                     SVIEIGEKLILNTNTITPLLKRMETMGLITRVPSETDHRKVMITLTAKGKAMKEPAAE
                     IPLRLVKKLNIDVEKANIDEINQLKSKLSQLISLLKKIEQ"
     gene            7350..9752
                     /locus_tag="PKH68_00980"
     CDS             7350..9752
                     /locus_tag="PKH68_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX11537.1"
                     /translation="MKKYLLLIVLLIMSLSTFAQLGVVKGRVFNAKTNEPIEFATLQI
                     EGTTIGSTSDLDGNFIFTGIKPGFIKLIARSVGFETTVSPEVQVQGNQTAFVDIAVPE
                     AAIVIKEVVVRQNLNIKRIESPISVLSVGVQEIEKSAGANRDVSKVIQLLPGVGATDP
                     NRNDLIVRGGGPSENVFYLDGIEIPVINHFATQGSSGGVVGVINPDFVREINFYTGAY
                     PANRTNALSSVMEIKQKDGSKDRLHTKLSVGASDAALTLDGPIGKKSTFIVSARQSYL
                     QLLFKLIGLPFLPTYNDFQVKYKYQIDQKNELTFVGIGAIDNMTLNTELEKTGTESQK
                     YLLSYLPIYKQWNYTVGVVYKHFGDKYIDNWVLSRNMLRNNNFKFKGNDDTLPKVKEN
                     LVSDYTSDEAENKLRFERDYPYLPVKLKVGGGLRYSHYTNETFRRLYFNNTVNELNYQ
                     TDLNLFGYQAFAQASDEYMNNRLKLSAGLSIVGNNYNKNMSNPLNQFSPRLSASYALN
                     EKLDLNANFGRYAMQPAYTTLGFRKETGGPLANQNEKLKYVISNQAIMGIEYRPAEKM
                     RLTVEGFYKQYENYPLSVSDGLSIASKGTEYGQVGDEEIVSSGKGRAYGVEILYKIME
                     WKNLNITSTYTFFRSEFTDKNNVYRPSSWDTRHLLNLISSYKFGKSWNVAMRWRFVGG
                     APYSPIDLEKSGNRSAWNITNQAYIDYNNFNSLRLPNAHQLDVRLDKEFYFKKWVLNL
                     YTDIQNAYNFQTKGAPIYTNKDVNGNILPDPNGDPARQGLREFEAYSGTILPTIGLII
                     KI"
     gene            9782..10510
                     /locus_tag="PKH68_00985"
     CDS             9782..10510
                     /locus_tag="PKH68_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11538.1"
                     /translation="MKTRILIAAILLMAVSASAKKKPDTFSSYIYGLNDQCTVLSLKF
                     EKGKEHNHPLFAVWLADENGKYIQTLYVSKSVGKGYFEHGSRNTGRWMPGEIQRPATL
                     PYWAHQRGVLNELGTYMPTLKNPVPDAYSGATPLSSFILNVKTDKKLSGKYRIYLEIN
                     QSWDWNEYWTNNKYPDDKEYKTSSQPALVYSADFDTSQAGTEIELKPVGRSHYAGSDG
                     QLYTDLNTLTTALKIAKKITLTFN"
     gene            10585..11082
                     /locus_tag="PKH68_00990"
     CDS             10585..11082
                     /locus_tag="PKH68_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNX11539.1"
                     /translation="MFTITVFSACSTIPKGAVAVSSFEKEKYLGKWYEIARMDFRFER
                     NMNNTTAMYTANKDGSIRVENAGYNYKKNVWKKAIGKAKFVGKEDVAMLKVSFFGPFY
                     GGYNVIALDKDYKYALIAGGDLGYLWILSREKSIPEEIKSDYLKKASDLGFNTSELIW
                     VEHNK"
     gene            complement(11233..15480)
                     /locus_tag="PKH68_00995"
     CDS             complement(11233..15480)
                     /locus_tag="PKH68_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX11540.1"
                     /translation="MKKFYFILALILLHSGIMYCQVPVVAKYIFNNFTVAESLPHNFI
                     DDLYKDSRGFIWISTGGGGLVRYDGYEFLTLNVSSFPVSLKSNYIRQVSEDNFSRLWV
                     ISSRGVDVINLRTMQNITNNPDNPDNLQMEKLSGINAQSVMKDQNGSIWILSVEKIYK
                     VDFDKNGNIENVLSSEKKIGEKSHTFTTINEIDNHIYVGNRGIIYQVTVPKNGSLTFK
                     PLNELLDFGENTFISTILKKDNTLWIGTDNGLYKFMINSNNLERYVHHENDLNSLSQD
                     MITTLAFFDDGTLVVGTLRGLNFYDAEKNNFSRISAKTGSKTSLSNDFINCLLPDRGF
                     LWIGTEAGGVNKMMLPKLITQNYVNNTDNPTSISPNPVNAIFEDKSGNLWLGTVEGGL
                     NRKLKGSNSFIHYTTANTNLSHNSVSAIETDGDDNLWAGTWGGGINIINLNRLTKPTF
                     TQLSFPTQYIAVLKYDSINKGMWIGTNRNIFFYDFELKKIINPLYVPQLQHIWGTLGC
                     LIDENKNLYIGTSEGLAIIQLNSYNRKTSKFKSVNFTGNGEKIASLFLRNITSMSMGT
                     DKSIWMGSNGYGICKMTRSKNKYKLQFFDKENGLGHNTVFGIVGDENGLIWISTGHGL
                     SCYNPANNHFANYTTNDGLICNQFYWNAFFKSPTNKNIYFGSNEGLTELMGIQQNSRS
                     NAGKIIFTKLQILNKTVWFGKNRYLKSDIAYADKVNLHEKDKSFSIEFAALGYDTPST
                     VSYAYRLLGFDDKWINTGADRRFITYTNLQPGKYRFQVRAMSRGNDWMDETAQLEIVV
                     HPYFYKTIWFLSFFVALILWGIYRIYKWRIGSLKSQQELLQQKVERRTNELQNQKKTL
                     EIQADELMKQNEILASQNKKIDAQRKQLIEMSAKVQEAMTDRISFFTNITHEFRTPIT
                     LITGPIERALKLSTNPKVIEQLHFVSQNSKHLLSLINQLMDFRKVESDKMSIQTKNGN
                     IISFLDEILVPFDPYTSERGIKIRRIYRLNSPEIMFDDEAIRKLVTNLLSNSIKFTPD
                     NGSITLYVCSLNDTDNKREKLYISLKDTGFGIDENEIDQIFDRFYQSKKHEKLSVSGQ
                     SGTGIGLYLCAKIAELLGGTISAKNNRGGGATFRVLVPLERSHNPILSEELPRNEVVN
                     AEFEHSETTAYLSTGKPTILVVEDNKDMRQFISSILCDDYKTIDAENGIEALQILKSK
                     TIDFIVSDLMMPVMDGLELSKQVKSDFSISHIPFLMLTAKSNVETQINSYKLGVDDFL
                     AKPFDPELLLARISNMLEARRNYQRKFSLNMDVKELNIREETNDEKFIHQAIQTVKEN
                     YKNPNYEVADFISDMGISKSLLNKKMNILTGQPAVQFIRNYRLAVAHELILKKKGNLN
                     ISEIAFEVGFNDPKYFTRCFAKHFGVAPSMLSRDEVK"
     gene            15846..17387
                     /locus_tag="PKH68_01000"
     CDS             15846..17387
                     /locus_tag="PKH68_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HNX11541.1"
                     /translation="MKHTLLIYVLLAIGIQVSAQQKAEVTVRVDKGEYQINKHIYGHF
                     SEHLGRGIYGGLWVGENSSIPNINGVRKDIIDALKQIKIPNLRWPGGCFADEYHWMDG
                     IGPRSERPKMVNTHWGGVVEDNSFGTHEFLNLCEALETEPYICGNLGSGTVEEMSKWV
                     EYITSDGESPMSNLRKKNGREKPWKVSLWGVGNENWGCGGNMTAETYAENYRRYATYC
                     RNYGDNKLFKIAGGPNVDDYRWMDVMMRNIPHFLMNGVSLHNYTFTRAWEDKGNATGF
                     SEDDYFSLLQHGMRMDELVTRHAEVMDKYDPKKSIGMVVDEWGAWYNVEPGTNPGFLY
                     QQNTLRDAILAGTILNIFHKHADRVQVANIAQMVNVLQALFLTDGDKMLLTPTYYVFD
                     MYKVHQDATMLPVEFNSPTYSRLGKSIPSLSASASRDKNGAVHVSFVNIDPAQSVEIS
                     CSLKDVKGKLISANIITAPKIDTYNTFENPNQVVLKEFKNGSFKNGTLKIKIPEKSVV
                     TVEIK"
     gene            17400..18881
                     /locus_tag="PKH68_01005"
     CDS             17400..18881
                     /locus_tag="PKH68_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HNX11542.1"
                     /translation="MKRIVQMMSVCSLFLFASCSSKTFEPVTTSNPWKDDYRDISSMK
                     DYAKWGTYNVHDPSCKLFGDTYYMYSTDAIFGENKKEIQESNLPFGNIQVRKSKDLVH
                     WEFAGWAFSEIPAEAKNWVLDKSGGRGATNIWAPYILQFNGKYRLYYCVSAFGKQTSC
                     IGLAESGSPEGPWTLKGCVVKTKTGDKMNAIDPSIVVNPETGEHWMHYGSYFGGLYCV
                     KLNPETGLTMHEGDQGHLIARRFNGKKNNIEAPEVIYNPELKKYFLFVSYDPLMTTYN
                     IRVGRSDKPEGPFLDFNGKDMREEEDNLPILTYPYQFKGHKGWAGTGHNSIFQDKSGA
                     YYLASQARLSPENHLMDLHVRQVFWTKDGWPVLSPERYAGTGQINIRKIDLAGEWEVI
                     TIHGDVPLRNLEAGQILWGENHLKSEEVNRSVVVRLNKDCSISGEMTGSWSYQNGILK
                     LKLHDKTTEVQVFAGQDWENEKKTLLFTGLNDKGYSIWGKRIK"
     gene            19294..21009
                     /locus_tag="PKH68_01010"
     CDS             19294..21009
                     /locus_tag="PKH68_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11543.1"
                     /translation="MKTTKLLKFALYFAILAAVGLVGCEKLDLYSIDAPSDLQSRIDS
                     IAAAKPNTGDTTYVTITTAIVGAENNSSGWWTAFSDYFSVPTGKLLHLEFINHGTGVN
                     NWNNWNLAVTNEIADRAAADYKEYFVLRSDAYGWGGKMANVDPRYAFDIKMITHNYPD
                     TDGNGDIWNDFRTTMQGARVSLDIDHSATGNVFVTATAVGTNGVTLVETYQQPVSAAA
                     DLMAFLICDGSYFEMKKAYLIPSKVTVVEDVNPVSIAVTGTPAFLELGNKNFWGDAKA
                     TVTFADGSSAQVDSADISFNVIPDMTTLGVKTVVVAYSKTKQGAYCKAVTTFYKLEVT
                     NPVVGLEVTTMPLITKYYYFSGDPILFNTTGLVVTGTYSDGTKAVIPKETLQFGKIPA
                     ASGTQSALISYVGATKTVTTTCPLTLVKGIGQVGANDFSTAWWTAFSADYKVPSGSSK
                     TLTMYCYSDNLANWHSPCTILRKSDKTENAVVRMDSFGWGAGYGTATLTSDWNWDKFT
                     SNISGSKVVITVTNNGNNTADVLYNVTYATGETHFQKYAGVTVDSADLNCALVTEVSY
                     LVIVE"
     gene            21125..24232
                     /locus_tag="PKH68_01015"
     CDS             21125..24232
                     /locus_tag="PKH68_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX11544.1"
                     /translation="MNQTNNITVKGKIVDDKGEPLIGATVQQKGTTVGVMTNASGNFS
                     LTVPSDASLVVSYVGCLTQEVFVGGKTDIGIITLASDVKELEQVVVIGYGTQRKVDLT
                     GSVAIVNAEEMKKVSHSNISTMLEGKVAGVQITSDGQPGADPTVRIRGIGSFGSTAPL
                     YIVDGVPMGTTIRDFSPNDIETLQVLKDASAAAIYGSRAANGVVIITTKQGKKNQAMK
                     IDYTGYTGFDQVQKGVYNVMDSYQYGKYVTMAYNNSGMDVPAGYNPASPKYVDPAQVN
                     TNWFNEAFKTGIRQNHNINMSGGGANNTYNIGLDYYSQKGTMVGAGPNFDRFTARVNN
                     AMDVKFVKIKTNMVYSHSNQDNMALSNANEYVQGLYGAQFPVMASALLLPPTIKAYDP
                     TTWVLDDKISAASEYSYDSYGFGTYYDDVHGDLRATNLLLTNSLLKRNSVVDRVVASG
                     SATVDLIGMVGQKTSNHKLDYNLNLSYSKTLVKDFTFVPAFIQSTTNYLSKSNENLSE
                     GYRSYSDALIENTLTYDGKFGLNHINTVVGQTFQRELFHTLTGKGVNLPEPYYLQVNN
                     AEETSSASNESEHVLASYIGRLNYDFDQKYLISATVRRDGSSRLSSADRWDWFPSISA
                     GWRIDRESFFPVAKTNINLLKIRGSYGVLGNENIGEYQYMDVMSRGNYTYSFGNNKVT
                     GSAISNYVNTAIRWEKKKTVDLGVDLGMFNNQLEFTFDWYKSTSEDLLYSVAVPTNAG
                     ATNGTVTMNAATMENSGLEFLLAYHNHKHPVKFDISTNLSTINNKVIKLGVSGEPRSD
                     GYCRTEVGREVGSFYGYVYDGIFQSQAEIDNRLNSEGEHINQSGAQPGDVAYADLNLD
                     GEITNEDQKYLGSGLPKFNYGLNARAEWKGLDLSISTYGVAGFKAVDFVDVTLHNSYG
                     ALNKSVDLLNAWTPENTNTNVPRVAYKSTGSITNDMFSERFIQDASYLKIANIELGYN
                     LPDRWFGGYVSGLRIYASAQNLATLSKYKGYNVDFAGGTFTPGYNYCSYPTPRTMMFG
                     IHCSF"
     gene            24263..26017
                     /locus_tag="PKH68_01020"
     CDS             24263..26017
                     /locus_tag="PKH68_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX11545.1"
                     /translation="MKSLNIILTTLVLLGGMIACTDQLNVQNPNNQTTYDFGNSESEL
                     QEAVISCYNRIRLEGTFARVGYTLDAVRGDEVWNSSQQWYLEYDNLNSPGTIGIGDEW
                     PWRDNYHVVNRTNFVLSKVDGVKMSVDSYNQIAGQALFLRSLAYYHLATYYQTVPLIT
                     DYASYSDINTMYASNATQDEVFDQIETDLKKAMEMLPSRDKGGEWAKGRATSGAAAGY
                     LARALMFRHKYAEAYAVLKDIIAGKYGHYELTADYGDNFREGPAFENNKESLFEVQFL
                     DFGTGGTDEEWTPVNISSEATQGHAVESNYASQELGSWGDLAGSPWLYNLFKKENSTD
                     GRLDPRLYWTLVTYENEYNTYKGLKTAAYPNGDPRSNTIYQKDITKTPLSNNAQGGIS
                     IAKFTNARNNIYSSITNGLHCGVNLRLMRYSDVLLRAAECENEISGPTQTAIDYINTV
                     RRRVALKDLKLSDFPSADALFEQIANVERPKEFGCENGRGIDLLRWGFFYDAGRLNQL
                     VEHGYYKRDGKASTEELTAETADDSSFKYYYKGHEYFPIFQSTLNANPNLVGNSANKN
                     EDNGPAFKAKWNVRPVVK"
     gene            26105..>26301
                     /locus_tag="PKH68_01025"
     CDS             26105..>26301
                     /locus_tag="PKH68_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HNX11546.1"
                     /translation="MRLRYILILSISITALSFVGCKPDEPKPDDKPNPFPTFTIPTYA
                     DDYSSISSWANRNKWNLANVH"
     assembly_gap    26302..26401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26402..27918
                     /locus_tag="PKH68_01030"
     CDS             <26402..27918
                     /locus_tag="PKH68_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HNX11547.1"
                     /translation="SISSWANRNKWNLANVHDPSVAYYKGYYYMYGTDASYGNAHDGH
                     GHFQGKRSTDLVNWDWVGGPFYQAPTWVADSLNAIRSRMGLDVIAKDKIEYGYWAPVV
                     RRVNVGGQEILRMYYSIVIFNYIKTGNLSSSPFDGSWTERSFIGMCESTDPAGAVWTD
                     KGFVTTSSSDRGKNFSRASTNDWSAYFYYNAIDPTYIVTPEGKHYLIHGSWHSGFALL
                     QLDATTGKPINKLGEPYASSASELTTRYGVRIGTRTAGSRWQGSEAPEIIYKDGYYYL
                     FMAYDGLDVPYNTRVVRSANIEGPYLDITGRNFTNGAGDSYPIVTHPYKFNLSNGWVG
                     ISHCAVFKKENTNEWFYMSQGRLPANTGGNPYSNAIMMGHVRRIVWCPASINEPDNLW
                     PIALPERYAAVPDYGTITKDSLVGTWEHINLKYSYAQQDLATALTLKADGTMSGALSG
                     NWSYDATKKQLILGNIVVCVEREVDWEATPRRVTFVYAGTEKNLNATYWGKKSK"
     gene            complement(28352..>29114)
                     /locus_tag="PKH68_01035"
     CDS             complement(28352..>29114)
                     /locus_tag="PKH68_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11548.1"
                     /translation="RQDYSNMLSYDLGLIYGNEFDKENEKPYDYFAFKLGGNFFSTQP
                     IVSRVNVLGMLFSTNVSLKSQNQCLNYGLFQHFNYYESQSDANHVQLYPYKISEAASA
                     GPGLLYKISAKNKVTFTASAYLSAILLGGSQTDHYRYDERDYNMGSGFSTKLNLKFNL
                     YEKLQIQLNTEDYRIYSWVGYGPNDFKEFNSSVHGDVGNTSLSVGRLGIGYLIGKNIF
                     ITSESSYNYRNSVYKYYPNVAHHVAEHKLSLGYLL"
BASE COUNT         8611 a         6321 c         6150 g         7832 t
ORIGIN      
        1 aatccatcgg taaaaacacg ctccagtccg ccgtgatgca gataaatatt tcgcagcgaa
       61 cgaagaaaaa actcacagtc cgcaccattg aacgtacgat gaataaagct gctcaattta
      121 ccaaaatcat ttccttccat tataaaatca tacggtgaat tatccatcaa atccatcagc
      181 cgctttgcat tgttgatgat cgtttttcgt tgtccccagg aaatggaagc tgccagaaat
      241 ccggcaattt caatatcttc ttttcgggta aaccggtgcg gaatttgaat gggatcattc
      301 tcaataaaat caatgtgatt gtatcggatt acagcttcat ccaataacgg tttaatattt
      361 gctccccaac ttcctgcagc atccaggact aacgggaaag tgggagtaga tgagtgaaaa
      421 ttattgatct cttgcttatt ctgcattttt caacttgaga ctttatggaa caaagtaaaa
      481 tttcttcact gttttattac ttcccttcag gggttggggg tctttgcctt ttttctccat
      541 aaactcaatc ccgaaagtgc caacaccaga aaaacggcgt actgaaaact tgtaaagtaa
      601 aatccctgaa tgagaaaaat tggtattgaa accacatttc cagcaatcca gaaatgccag
      661 ttttcgagtt tttttatcgt catcagtatg gtagcacaga ggaaaagcga gctgttcagg
      721 gcgtccatcc agggcataga agaatgaaga taggcggcgt ctgcacggtt gtaccagcgg
      781 agtaacagca aaacagcgac gtaaaacaca atggcaaacc cgatgatgag ccagttttgt
      841 cgggaagtgt ttctggtgat tgttaccaca tgctcttcat tttttttcct cgaccagttg
      901 taccaaccga atgcattggt aaagaagtaa atgaggttga taccggcgtt ggcgtacata
      961 cgtcccgtaa aaaacaggta gataaaaatc agcacactga taatccccat tgggaatacc
     1021 agaatatttt ccttccgggc aaaccataca ctcgctattc caaatacaac ggctataagt
     1081 tcaggataat tcattccttt tcctccttat ttggcttcat aactactcct ttgtttttac
     1141 gaccatccac tttcaccact atcggatgtt cgaaatgcac atgccggata tattccgact
     1201 cgaactcagc aggttgtgca ttcagaaaat cgatatcata cgatccgtcg ttctgaaaag
     1261 gattgatggt gaaataggaa acgccaaatg aagtcaggtt ttggaaaaag tgcgtaccct
     1321 gacttggatc aatccggtaa ctggacagac cgctttctac tatcacccgg gcattggaga
     1381 tatgtggcca cttgaccgga atgcccagcc atacatcgct cgagccccac cgcccgggcc
     1441 cgaccaatac ataatatcgt tcctgctgaa gcattttttt gttcagtttt tcaatatcgt
     1501 acatgatcaa ctgattttta gaggcgttga aagcttcggg tttcacatac acaatatcgt
     1561 aaatgtcgtt agtgattcca tgtcccatgg cattctcgca gctgatgatg gtatcagcct
     1621 cctgaatggc accgatgtct tcactgatca tttctttgct gtccacgatg ggacgtatct
     1681 gcagcagata gaaagtgtgg gtaggttttg gcgaataatc aagcgttacg gcaaattcaa
     1741 tctcgatagg tcgtcccatt tcagcgtgag caatccttaa tacttcctgc ataattttgg
     1801 gcagcgggaa tacattgtgc tgcaatatat tggcaaacgt catcacgcgg cgtccttctt
     1861 caaaaacgct gtcgtatatc acctgatcgc gcgggttgaa ggtagaagcc atatatttca
     1921 gggtgccatc cttatcggct tcctgtaccc tcactttcag caggttaaat ccgtcatcca
     1981 cctcgggaac aaagtttttt tcacgggcat ccagcgcatt gaagtaggtt tgtgtttcac
     2041 gcagcgccat atccatcgtg ctggtttgca gcacattgtt aggatgatag ggtgaaaacc
     2101 gaagggtaac tccgccgtcc acaatgtatt tacccaatcc catagcaata tttacgatac
     2161 cttcttcggc cttttcggca cccagcggat aaaagttgag tgaccgcgct actccggaaa
     2221 aggatggata aaatcgttcg ccgtgataac tgccgcacgt ctcctggagc acgatggcca
     2281 ttttttcctc atcaattaca ttcttcgttg cggtcatata agccttgctc gaacggtaaa
     2341 aaaccgatgc gtaaacggct tttacagctt cggtgatcat gcgaagcata ttagttttac
     2401 ttttgccatc atatggaacc atataggtgg aataaattcc ggcaaaaggc tgatagtggg
     2461 agtcctcaag cagactggag gagcggactg ccaccggcga actgattgcg cccaaaaaag
     2521 catacaggtc ggcaataatg cggcggggca atttagcttt gaggaaatgc tgaagaatta
     2581 cctcgtcgtc cacatccgac agagcgatgg gatagagttc attcatttcc atgaattcgg
     2641 taaaattatc ggtacacagc accacggtgc ggggtatgca aatttgggta tcgcgaaaat
     2701 tctccagact ttcgttggtt ttgatcatgg catcgagaaa tgccagtccg cgccctttgc
     2761 ctcccatgga accttcaccg atacgggcaa aattggaata ctggtcaaac cggtcacgct
     2821 gaaaaacggc cacaataccg cggtttttta ttcgccggta cgtaactatt gcatcaaaaa
     2881 taatctgacg cactttattc atatccgtct tttcctgcac gtccacatgg atattttcaa
     2941 gaaattgccc gaggggaaac atggcccgtg aatagagcca gcgcgatata ttgttgcgcg
     3001 aaacatgata aaacaaggta tcgtcgctga ttttgaaaat aatatcctgc aggccgcgca
     3061 ggttttctat tcgcgcctct tcttcgcccg taaccgggtt ggcaaataca aaatcgccaa
     3121 aaccaaaatt attggtaatg atttcgcgca attccatcat ttgtgttttc gacattttgt
     3181 tgacaaatgc cgctttcacc tcatcggcca gcttgtgatt attctcgttg gtggactgaa
     3241 caataagggg aataaacgtg tcctgtttgc ggatttcacg gcaaagttca attccggctc
     3301 ccgaattttt gacaccgttt ttcttataat tcacatcggt aatcacaccc agcatattgc
     3361 gcttatattt ggtgtaaatc tgcatagctt cctcgtaatt gcgggcaagc aggattttgg
     3421 gacgaccacg cattcgcagc atctgttcgt gtgcattgag tgcttcggtc atgaacgaac
     3481 gcgactgaac aaacacatac ttgtacaaat gaggcagaat ggacgaataa aaccgcacag
     3541 aatcctctac aaaaaggatg acctgcactc ccaccgactc tacatcttct tccacattca
     3601 ttgcatcttc tatcaatttg acaattgcca ataaaagatc cgaattaccc agccagctga
     3661 acacataatc gatggcactt aaatcttcgt tggctatgcg ctgcgaaacc attctcgaaa
     3721 aaggggtgag aactacgatt ggaatgtcgg gatgggtaat tttcacctct ttggcccaac
     3781 tgaagggatt gatactgtca ccgctgggca tggaaataat aagctcatac gttctatctt
     3841 tcaatgccag attggcttcc tcttcgttgg ccaccagcgt aaaacgtggc ggataacgca
     3901 aattcagtga aacgtattcg ttgaaaatct gctcatccac ccgtccgtct tcctcaagca
     3961 tgaaagcatc gtacttactg gcatagagca gaatattata gatacgatgc ctcatcagac
     4021 tggcaaatga agtatcttta aaatagagtt ttttaatact gttggtattc atagttctgt
     4081 gatggataga tgagacacgt ctgttataaa tatcacgtca aaattgatca ttttgctgaa
     4141 attcccggtc ttatcaatca aattcttatc atttatcctg tttagataat aatatgctga
     4201 taatcagaaa aacggtaaat atttatttag aaaaatattt ctcaaaaata cttcaaaata
     4261 tttgattatc acaaataaaa catttaaact tgcagtgtaa tctaaatgta agtaaccgaa
     4321 atgttcattt tgtcatttag gaacttaatg attaatttta ttgcagaata atgcattttt
     4381 actatctttt tgttatttat cttaattttt tcaatcaata aaactgaaag cattttttta
     4441 taaaaactca aaaaaccgga attttcaatt atcaatacat ctaactaaat aaatctaaca
     4501 atcatgattt tggaaaaatt aatgcaacaa ctcgaagcta agcatccggg tgagcaagaa
     4561 tacctgcaag ctgtacgtga agttctgtcg accatcgagg aagtttataa cgaacatccc
     4621 gaatttgaaa aagcaaaaat cgcggaacgc ctcgttgagc ccgaccgtat tttcactttc
     4681 aaagtgccct gggttgatga ccgcggtgaa gttcagctca atatcggtta ccgtgtacag
     4741 tttaacaatg ccattggccc ttacaaaggc ggtttgcgtt ttcacccctc ggttaatctt
     4801 tccatcctta aatttctcgg ctttgagcaa attttcaaga atgcactgac cacactgcct
     4861 atgggtggcg gtaaaggtgg atcggatttc aatccacgcg gaaaatcgga tgccgaagtg
     4921 atgcgcttct gtcagtcatt catgcttgaa ctctggcgac acattggtgc cgataccgac
     4981 gttccggccg gcgatatagg tgttggcgga cgagaaatag gttatatgta tggcatgtac
     5041 cgtaaactgg ctcaaatgaa taccggagtt ctcaccggca aaaacctgga gttcggcggc
     5101 tcgctcattc gtcccgaagc taccggtttc ggagcccttt attttgtaaa acaaatgtta
     5161 gaaacagctg gtcaggatat taaaggtaaa acggtggctg tttccggatt tggcaatgta
     5221 gcctggggag ccatccttaa agctaccgaa atgggcgcca aagtggtcac tatttcggga
     5281 cccgatggat atatttacga cgaaaacggc attacaggca aaaaaataga ttacctgctc
     5341 gaactccgtg ctacctgcaa cgacgtagtg gcaccttatg ccgaagaatt cggtgccgtg
     5401 ttcgttccaa accgcaagcc ctgggaagtg aaagtggaca ttgccctgcc ctgtgccacc
     5461 caaaatgaac tcaacggtga tgatgcccga aaaattatcg aaaacggcac tttctgcgta
     5521 gccgaggttt cgaatatggg ctgtacaccc gaagctatcg aattgctcat cgaacataaa
     5581 atcatgtacg gaccgggcaa ggctgtgaat gccggtggtg tagccacctc cggactggaa
     5641 atgacacaga atgccatgca catatcctgg ggagcccccg aagtggatgc cagactgcaa
     5701 cagatcatgc acgatattca cggacaatgc gtaaaatacg gcagacaacc ggacggctac
     5761 attaattata tgaaaggcgc taacgtggcc ggtttcctca aagttgccaa agccatgctt
     5821 gctcagggaa ttgtataaac cacttttttc atcaacatat agtttattct accaaaaagg
     5881 tctcacagaa tgattgtgag accttttttt atttcatttt tcagagttcc tgcctacacc
     5941 tgcttacacg aaaaatttga aatagttcta tttcctccaa aatttcaggg aaaagccgca
     6001 gttttagtta tcattcgtta attatcacta attattactt ctttttatat cgcttgcgat
     6061 acatcgcagt atatttgtat cgcaaacgac ataaacaaat aaaaacaaca agcataatta
     6121 tgaaaccaaa cttttatcaa ttcagcgcaa cttctttaca aggtaaagaa atagacatga
     6181 aatcctacga aggaaaaata gtactggtag tgaatactgc cagcaaatgc ggtcttacgc
     6241 ctcagtatga aggactggaa aaactgcata aagaatacaa agacaaaggg ctggttatcc
     6301 tgggatttcc ctgtaaccag tttgccaatc aggagccggg cacagccaaa gacatatctg
     6361 aaggatgtct gattaattac ggcgtaagct tccaaatgat gtctaaaatt gacgtgaacg
     6421 gacccaccgc acatcctatc tacaaatatc tgaaaaaaga actgaaaggt tttccgggga
     6481 gtgaaatcaa atggaatttc ggaaaattcc tgattgacaa agacggtaat ccttacaaac
     6541 ggttttcgcc actcacaaaa cccgaaaaat tacggagtga tattgaaaaa ttactggcaa
     6601 aataaatccg ggaattttaa taatcgaatt ttattctaca aaatttttca aaaaaaaaag
     6661 agaccaatga cctacgaaca actaaaactc gataaccagc tttgctttcc tttttacgcc
     6721 gtatcgaggc tgataacaag ggcctatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnaa ccagctttgc tttccttttt acgccgtatc gaggctgata acaagggcct
     6901 atcaggatga cctggatgcg ctggggataa cttacccgca atatctggtg atgatggtga
     6961 tgtgggaaca cgaggagctg tcggtgatag aaataggtga gaaactgata ctgaacacca
     7021 acaccatcac tccactgctc aaaagaatgg aaacaatggg cctgataacc cgtgttccgt
     7081 ctgaaaccga ccaccggaag gtaatgatta cgctcactgc gaaaggaaaa gccatgaagg
     7141 aaccggccgc cgaaataccg ttgcgcctgg tgaagaaatt gaatattgat gttgaaaaag
     7201 ccaacatcga tgagattaat caattaaaat caaaattatc tcaactgatt tcacttctta
     7261 agaaaataga acagtaaatt catacctgat tgcagtttca ttaaaaatcc tttatattta
     7321 caaaacatta atttaacaat taacgtaaaa tgaaaaaata tcttctgctt atagttctgc
     7381 ttatcatgag tttatccacc tttgcacagt tgggggtggt aaaaggacgg gtattcaatg
     7441 ctaaaacaaa cgagccgata gagtttgcca cgctgcagat tgagggaacc accattggat
     7501 ccacttccga cctggacggc aactttattt tcaccggtat caagcccgga tttatcaaac
     7561 tgatagcgcg ctcggtaggt tttgaaacca ccgtatcgcc tgaagttcag gtacagggca
     7621 atcaaactgc gttcgttgat atcgctgtac ccgaggctgc gattgtcatc aaagaggtgg
     7681 tggttaggca aaatctaaac attaaacgga ttgaaagtcc catatcagta ctatctgttg
     7741 gtgttcagga aattgaaaaa agtgccggtg ccaaccgcga tgtgtcaaaa gtaatccagt
     7801 tgcttcccgg tgtgggcgcc acagacccga accgcaacga cctgatagtg cgtggtggag
     7861 gcccttcgga gaatgtgttt tacctggatg gaattgaaat tccggttatc aatcactttg
     7921 ccacacaagg ttcatcgggt ggtgttgtag gcgtcatcaa tcccgacttt gtacgtgaaa
     7981 tcaattttta caccggcgct tatcccgcca accgcaccaa cgcgctgagt tcggtgatgg
     8041 aaataaaaca aaaagacggc agcaaagacc ggttgcacac caaactgtcg gttggcgctt
     8101 cggatgcagc tctgacactg gatggcccca tcggtaaaaa atcaactttc atcgtatcgg
     8161 cacgtcagtc gtatctgcaa ttgctcttca aacttatcgg tctgccattt ctgcctactt
     8221 acaacgactt tcaggtgaaa tacaaatatc agattgacca gaaaaacgag ttgactttcg
     8281 tgggtatagg tgccattgac aatatgactt taaatacaga acttgaaaaa acaggaaccg
     8341 aatcacaaaa atatttactg agctaccttc ccatctacaa acagtggaat tatacggtgg
     8401 gtgtggtgta caaacatttc ggcgacaaat acattgacaa ctgggtgctg agccgcaaca
     8461 tgcttagaaa caacaacttc aagttcaagg gaaatgatga cacattgccc aaagtgaaag
     8521 aaaatctggt gtcggattat acatcggacg aggctgaaaa caaactgcga tttgaacgcg
     8581 actatcctta cctgcccgtg aagctgaaag ttgggggtgg tttgcggtat tcacattaca
     8641 cgaatgaaac attccgccgc ttgtatttca acaataccgt gaatgaacta aactatcaga
     8701 cagatttgaa cctttttggt tatcaggcat ttgcacaggc atcggacgaa tatatgaaca
     8761 accggttaaa attgtcagcc ggactcagta tcgtgggcaa taattacaac aagaatatga
     8821 gcaacccgct gaaccagttt tcaccccgcc tttcggcttc gtatgccctg aatgaaaaac
     8881 tggatttaaa tgccaatttt ggacgctatg ccatgcaacc ggcttatacc acactcggat
     8941 ttaggaaaga aacgggcgga ccattggcca atcagaacga aaaactgaaa tatgtaattt
     9001 caaatcaggc tatcatgggt atagaatatc gcccggcaga aaaaatgcgt ctgactgtgg
     9061 aaggttttta caagcaatat gaaaattatc cgctttcggt ttcagacgga ctgagtatag
     9121 caagcaaagg taccgaatat ggtcaggtgg gcgacgaaga aattgtatcg agcgggaaag
     9181 gtcgtgctta tggtgtggaa attctctata aaatcatgga gtggaagaat ctgaacatca
     9241 cctccacgta cactttcttc agaagcgaat ttaccgataa gaataatgtt taccgtccgt
     9301 catcttggga tacccgtcac ttgttgaatc ttatttcgag ctacaaattc ggtaaaagct
     9361 ggaatgtggc catgcgctgg cggtttgtgg gaggtgcccc ctattcacct atcgacctgg
     9421 aaaaatcggg taaccgctct gcctggaaca tcaccaatca ggcctatatc gattataaca
     9481 atttcaattc actgcgcctg cccaatgcac accagctgga tgtacgctta gacaaagaat
     9541 tttatttcaa aaaatgggtg ttaaatttgt ataccgatat acagaatgcc tacaatttcc
     9601 agacgaaggg agctcctata tataccaaca aagatgtgaa cggcaatatt ctccccgacc
     9661 ccaacggcga tccggcgcgt cagggattgc gcgaatttga agcatacagc ggaacaattt
     9721 tacccactat tggtttaatt ataaaaatct gatttttaac gaataaaata ccaacaatcg
     9781 tatgaaaaca agaatattaa tagcagcaat actcctgatg gctgtgtctg cttcagccaa
     9841 aaagaaaccc gatacgttct catcatacat ttacgggctg aacgatcagt gcacggtgct
     9901 aagtctgaaa tttgaaaagg gaaaagaaca caatcatccg ctatttgctg tgtggctggc
     9961 ggacgaaaat gggaaatata tccagacact atatgtatca aaaagtgtgg gaaagggcta
    10021 ttttgaacac ggaagccgta ataccggacg ctggatgccg ggcgaaatcc aacgtccagc
    10081 cacactgccc tactgggcac atcaacgagg agtcctgaat gaactgggaa cgtatatgcc
    10141 aactctcaaa aatccggttc ccgatgctta ttcaggtgca actccccttt cgtcgtttat
    10201 tctgaacgta aaaaccgata aaaagttatc ggggaaatac cgcatttacc tggaaattaa
    10261 ccagtcctgg gactggaacg agtactggac gaataacaaa tatcccgacg acaaagaata
    10321 taaaacatcc agtcagcccg ctctggtcta ttcggcagat tttgacacca gccaggccgg
    10381 aacggaaatt gaactgaaac ctgtaggacg cagtcattat gcaggaagtg acgggcagct
    10441 ctacaccgat ctgaatacgc tgaccacggc cctgaaaatt gcaaaaaaga ttaccctgac
    10501 ttttaactaa atggctattt atcccacaaa ttaacacaaa acattatgaa attcaacaaa
    10561 ttaactctaa caacattatt agcaatgttt acaatcacag tattcagtgc atgctctacc
    10621 atcccgaagg gtgcagtggc agttagttct ttcgaaaaag aaaaatatct gggaaaatgg
    10681 tatgaaattg ccagaatgga ttttcgtttt gaacggaata tgaacaacac cacggccatg
    10741 tacacggcaa ataaagacgg cagcatacgg gtggaaaacg ccggatacaa ctacaaaaag
    10801 aatgtttgga agaaagccat cggaaaagca aaatttgtgg gaaaagaaga tgtcgccatg
    10861 ctgaaagttt catttttcgg gccattttac ggcgggtata atgtcattgc actggataag
    10921 gactacaaat atgccctcat agccggaggg gatttgggct acctctggat actttcacgg
    10981 gagaaatcaa tccccgaaga gatcaaaagc gattatctga aaaaagccag cgatctgggt
    11041 ttcaatacat cggaactgat ctgggtggaa cataacaagt aagacagtga tcctatttaa
    11101 taggatacgg gtaaatggct gatacgaaaa ttaagctgct atatcattat cttacagcat
    11161 gataaacaac agtcatatag cttcaatgga ataaaatatt cataaagcta tccgcactcc
    11221 gattaattac atttatttta cttcatctct ggagagcata cttggtgcca ctccaaagtg
    11281 cttagcaaag cagcgggtga agtatttcgg gtcattgaaa cccacttcga aggcaatctc
    11341 tgaaatattg agattgcctt ttttcttcag aattaattcg tgagctactg caagtcggta
    11401 gtttcggata aattgcacgg caggctgtcc ggtgagaata ttcatttttt tatttagcag
    11461 acttttgctt atacccatat ccgaaataaa atcggcgact tcgtaattcg ggtttttgta
    11521 gttttctttc acagtttgaa tagcctgatg gatgaatttt tcatcattcg tttcctcccg
    11581 gatattcagt tctttcacat ccatattcag gctgaatttt cgctggtagt ttcggcgtgc
    11641 ttcgagcata ttgcttatgc gggccaacaa caattcagga tcaaaaggtt ttgccagaaa
    11701 atcgtccaca cccagtttgt agctatttat ttgagtttct acatttgatt tggctgtaag
    11761 catcaggaaa ggaatgtgtg aaatggaaaa atctgatttc acctgttttg aaagctccag
    11821 accatccatc accggcatca tcaaatcgct gacaatgaag tcaatagttt tggatttgag
    11881 tatttgcagg gcttcaatac cgttttctgc atctatcgtt ttgtaatcat cacagagtat
    11941 ggaacttatg aattgtcgca tgtctttgtt atcctccact accagtattg ttggtttacc
    12001 ggttgaaaga tatgctgtgg tttccgaatg ttcaaattcg gcattcacca cttcatttcg
    12061 gggtaattct tccgaaagta tcgggttatg acttctttcc agaggcacca gaactctaaa
    12121 agtagctcca cctccacggt tgttttttgc tgaaatggtt ccgccaagca actctgcaat
    12181 cttagcacac aggtacaacc cgatacctgt accgctttgt ccggaaaccg aaagtttttc
    12241 gtgtttcttg gactgataaa aacgatcaaa aatctggtca atttcatttt catcaattcc
    12301 aaatccagtg tctttcagac tgatgtataa tttctcccgt ttattgtcag tgtcattcaa
    12361 cgaacacaca tacagggtga ttgaaccatt gtcgggtgtg aatttaattg aatttgaaag
    12421 taaattggta accagctttc ggatggcttc atcgtcgaac ataatttctg gtgaattcaa
    12481 ccgatatatt cgtctgattt taataccccg ttcggaagtg tacgggtcga agggaaccaa
    12541 aatttcatcc agaaaactta tgatattgcc attctttgtt tgaatactca tcttatcgct
    12601 ttccactttg cgaaaatcca tcaattgatt gattaacgag agcaaatgtt tggaattttg
    12661 agatacaaaa tggagttgtt ctataacctt cggatttgtg ctgagtttca atgcacgttc
    12721 tatcgggccg gtgataaggg taataggagt tcggaattcg tgtgttatgt tggtaaaaaa
    12781 tgagatacgg tcagtcatgg cttcctgtac ttttgccgac atttcgatca gttgttttcg
    12841 ttgtgcgtcg atttttttat tttgcgaagc cagtatttca ttttgtttca tcaactcatc
    12901 ggcttgtatt tccagagtct ttttctgatt ttgcagctca ttggtccttc tttccacctt
    12961 ttgctggagc aattcctgct gacttttcaa ggacccgatt cgccatttgt aaatacggta
    13021 tatgccccac aatattaatg caacaaaaaa tgaaaggaac cagattgttt tataaaaata
    13081 aggatgcacg actatttcca gttgagctgt ttcgtccatc cagtcgttgc cgcgcgacat
    13141 ggcgcgaacc tgaaacctgt actttccggg ttgcaggttg gtgtaagtta taaatcgcct
    13201 gtcggcaccg gtattaatcc atttgtcatc aaatccaaga agccggtaag catacgaaac
    13261 cgtggatgga gtgtcgtaac cgagtgctgc aaattcaatg gaaaatgatt tgtctttttc
    13321 gtgtaaattc actttatcgg cataagcaat atcgctcttc agatatcggt tcttgccaaa
    13381 ccatactgtt ttatttaaga tctgtagttt ggtgaaaatt attttgccgg catttgatct
    13441 gctgttctgc tgtatcccca tgagttcagt cagtccttca ttgcttccga aataaatatt
    13501 cttatttgtt ggagatttga agaaggcatt ccagtaaaac tgattgcaga ttaagccatc
    13561 attggtcgta tagttggcaa aatgattgtt agccggattg taacacgaga gtccatgccc
    13621 ggtacttatc catattaatc cgttttcatc gcccactatt ccaaacacag tattgtggcc
    13681 caaaccgttc tccttgtcga aaaactgaag cttgtatttg tttttggaac gggtcatttt
    13741 acaaatgcca tatccattac tgcccatcca tatcgatttg tcggtgccca tactcatact
    13801 cgtgatgttt ctcaggaaga gagaagcaat tttttcacca ttgccggtga aatttaccga
    13861 cttgaatttt gaggttttgc ggttgtagga attcagctga ataatggcaa gtccttcgga
    13921 tgtgccgatg tacaaatttt tattttcgtc aatcaggcat ccgagagttc cccagatatg
    13981 ctgcaactga ggtacgtaca gtgggtttat tattttcttt aattcaaaat cgtagaagaa
    14041 aatattccgg tttgtcccta tccacattcc cttgtttatc gaatcatatt tcaaaactgc
    14101 gatatattga gtggggaaac tcaattgagt aaaagtgggt tttgtcaacc ggttcagatt
    14161 gatgatgtta atgcctccac cccaggttcc tgcccacaag ttgtcgtctc cgtcggtttc
    14221 tatggcactt accgagttat gacttaaatt ggtattggca gtggtgtaat ggatgaatga
    14281 attgcttcct ttcaatttcc ggtttagccc gccctccact gtaccgagcc ataaatttcc
    14341 acttttgtct tcgaaaatgg catttaccgg attgggcgat atggaggttg gattgtctgt
    14401 attatttaca taattttgag tgataagttt tgggagcatc atcttgttta ctccgccagc
    14461 ttcggttcca atccacaaaa atcccctgtc gggcagcagg caattgatga agtcgttgga
    14521 aagtgatgtt ttgctgccgg ttttggctga aatgcgactg aaattatttt tctcagcatc
    14581 ataaaaattc aagccccgta atgttccaac cactaaagtt ccatcatcaa aaaaagccag
    14641 ggtggtaatc atatcctgcg agaggctgtt aagatcgttt tcatgatgaa cataacgttc
    14701 caggttattg ctgttaatca taaatttgta caaaccatta tcagtgccaa tccacagtgt
    14761 attatctttt ttcaatattg tggaaatgaa tgtattctca ccaaaatcaa gtaactcgtt
    14821 cagcggcttg aaagtcaaag aaccattttt tggaaccgtg acctggtaaa ttattcctct
    14881 gtttcctaca tatatatgat tgtcaatttc gttgatagtg gtaaatgtat gggatttttc
    14941 cccgattttc ttttcagagc taaggacatt ttcgatattt ccatttttat caaaatcaac
    15001 tttgtaaatt ttctcaacag atagtatcca gatgctgccg ttttgatctt tcatcacact
    15061 ttgagcgttg ataccggaga gtttctccat ctgcagatta tccggattat ccggattatt
    15121 cgtgatattt tgcatcgtac gcaaattgat tacgtcaaca cctctgctgg aaatcaccca
    15181 aagccggctg aaattatctt cgctcacctg acggatgtaa ttgcttttca gagataccgg
    15241 aaaactgctc acgtttaagg tcagaaattc gtatccgtcg taccttacca ggccgccacc
    15301 acccgtcgat atccatataa atccacgact gtctttatac aggtcgtcaa tgaaattgtg
    15361 tggaagagat tcggccaccg taaaattgtt gaaaatgtat tttgcgacaa ccggaacctg
    15421 acaatacata attccactgt gcaatagtat caatgcaaga ataaaataga attttttcat
    15481 atattaattg tttcccaaag caaaattaga ctattaatca gaaaaagtgt gatatttaca
    15541 cttaaaaatc aaattgtcca ccaattaagg aaaaatgtcc accttcatgc catatctgat
    15601 gacgtatatt tgcataaccg ttatagcgaa atttataatt ttgagcgaaa aatactgaat
    15661 ttttacggaa taaccttaag attaacacat atccggatag ttatttttac tgaccactta
    15721 tttgtcattt aaaaatttca aaaatgcagg cattcaacca taaatgcaaa tattttgtca
    15781 ataaaaaaag gagaataatc aggcaaagat taatataatc aaatatagca taaataaaaa
    15841 ttatcatgaa acacacatta ttgatttacg tattgctggc cattggaatt caggtttcgg
    15901 ctcagcaaaa agcagaagtt acggtacgtg ttgacaaagg tgaataccaa atcaacaaac
    15961 acatctacgg acatttttcc gaacatctcg gcagaggcat ttacggcgga ctttgggtgg
    16021 gcgaaaattc ctccattccc aacatcaacg gagttcgtaa agacataatt gatgcattaa
    16081 aacagattaa aattcctaat ctgcgctggc cgggaggttg ttttgccgat gaatatcact
    16141 ggatggatgg cataggaccg cgctctgaac gcccgaaaat ggtcaacacg cattggggcg
    16201 gagtggttga agataacagc ttcggtactc acgagtttct gaacctttgc gaagcactcg
    16261 aaacagagcc ctatatctgc ggaaaccttg gaagcggcac cgtggaggaa atgtcgaaat
    16321 gggtggaata catcacctcc gatggtgaaa gccccatgtc gaacctccgg aagaaaaacg
    16381 ggcgcgaaaa accatggaaa gtaagcctct ggggagtagg caacgaaaac tggggttgcg
    16441 gtggaaatat gacagccgaa acgtacgccg aaaattacag acgatatgca acctactgcc
    16501 gcaactatgg cgacaataaa ctattcaaaa tagccggcgg acccaatgtg gacgattacc
    16561 gctggatgga tgtgatgatg cgtaatatcc cgcattttct gatgaacggc gtttcgcttc
    16621 ataattatac atttacccgt gcctgggaag acaaaggcaa tgccaccggc ttcagcgaag
    16681 atgattattt ctcacttctg cagcacggaa tgcgcatgga tgagttggta acccgccacg
    16741 ccgaagtgat ggataaatac gaccccaaaa aaagtatcgg gatggtggtg gatgaatggg
    16801 gagcctggta caatgtggag cccggcacca atcccggatt tctttatcag caaaacaccc
    16861 ttcgtgatgc cattctggca ggaacaattc tgaatatttt tcacaaacat gccgaccgtg
    16921 ttcaggtagc caacatcgct cagatggtca acgtactgca ggcacttttt ctgaccgatg
    16981 gcgacaaaat gctgctcacg ccgacttatt acgtttttga tatgtacaag gtacatcagg
    17041 acgccaccat gcttcctgtc gaattcaact caccaaccta cagccggctg ggcaaatcca
    17101 taccttcgct ttccgcatcg gcttcccgcg ataaaaacgg ggcagtccat gtgtcgtttg
    17161 tcaacataga cccggctcag tctgtggaaa tatcctgcag tctgaaagat gtgaaaggca
    17221 aactcatttc agccaatatt ataaccgcac caaaaataga tacgtacaac accttcgaaa
    17281 atcccaatca ggtggtattg aaggaattta aaaacggaag tttcaaaaac ggaactttaa
    17341 agatcaaaat acccgaaaaa tctgtagtga ctgtcgaaat caaataaatg attgacaaca
    17401 tgaaacgaat tgttcagatg atgtccgtct gtagtctctt cctgtttgct tcgtgcagca
    17461 gcaagacctt tgaaccggtg accacctcca atccatggaa ggacgactac cgcgacattt
    17521 cctccatgaa agattacgca aaatggggaa catacaacgt tcacgaccca tcctgcaaac
    17581 ttttcggaga tacgtattac atgtattcca ccgatgccat ttttggggag aataagaaag
    17641 aaattcagga aagtaatctg cccttcggca atatacaggt acgcaaatcg aaagatctgg
    17701 ttcactggga gtttgcaggc tgggcattca gcgaaatacc tgctgaagcc aagaactggg
    17761 tactcgacaa aagcggcggc agaggtgcaa ccaacatttg ggcgccttac atacttcagt
    17821 ttaacggtaa atacagattg tattattgtg tttcggcatt tggaaagcaa acttcgtgca
    17881 ttggccttgc cgaatccggc tcacccgaag gtccctggac actgaaagga tgtgtggtga
    17941 aaaccaaaac cggcgacaag atgaatgcaa tagacccgag cattgtagta aatcctgaaa
    18001 ccggcgaaca ctggatgcat tatggatcgt attttggtgg tttgtattgc gtgaaactga
    18061 acccggaaac cggcctgacg atgcacgagg gagatcaggg acatttaatt gcacgcagat
    18121 ttaatggtaa aaaaaacaac attgaagctc ctgaagtcat ttacaatccc gagctgaaaa
    18181 agtattttct cttcgtatcc tacgatccgc ttatgactac ctacaatatt cgggttggtc
    18241 ggtctgacaa accggaagga ccgtttctgg acttcaacgg aaaagacatg cgtgaggaag
    18301 aagacaattt gcccattctc acctatccct atcagttcaa aggacacaaa gggtgggccg
    18361 gaactggtca caattccatt tttcaggata aaagcggtgc gtattacctt gccagccagg
    18421 cacgtctgag ccccgaaaat catctgatgg atttacatgt gcggcaagtg ttctggacca
    18481 aagacggttg gccggtgctc tcaccggagc gatatgccgg aaccgggcaa ataaatatcc
    18541 ggaaaattga tctggcaggt gaatgggaag taattaccat tcacggtgat gtgcccctga
    18601 gaaacctgga agccggacaa atcctgtggg gtgaaaacca tctgaaatcg gaagaagtga
    18661 accgctctgt cgttgtcagg ttgaacaaag attgttctat cagcggcgaa atgaccggaa
    18721 gctggtcgta ccaaaatgga attttaaagc tcaaacttca tgacaaaacc acagaagttc
    18781 aggtatttgc cggacaggac tgggaaaatg aaaagaaaac actgctcttc accgggctga
    18841 atgacaaagg ctattccatt tggggtaaaa ggataaaata accagaaaca aaagaaaaaa
    18901 catcattaat aatccaatta actctaattc tactttaaaa tgaaagacgt aacacattta
    18961 ttctcctcag gacaattttc cggcttcgca ttgtgtggtg agcctggcaa atttgatcca
    19021 cagattggag acggttcaaa aaaaggacct ccctgaaaag cactgttaat ttggcatact
    19081 tgaatggagc tgattatgtc gatacatagt aaagggaaag cagcattaac aattcattct
    19141 tactaatgcg aacaatcagt aaagtatgac ttttggatgt tattgaaatc aggacaattg
    19201 cccaaatcag gtaaccaaat caggttagta acaagactaa ctacccggaa ataattatta
    19261 aaaaaaagaa agaattcaat taaaattaaa tttatgaaaa caactaaact attgaagttc
    19321 gctttatact ttgcaatttt ggcagcggtt ggcttagttg ggtgcgaaaa attggatctg
    19381 tattccattg atgcaccctc cgacctgcag agcaggatcg actccattgc agctgcgaaa
    19441 ccaaataccg gtgacacaac ttacgtcacc atcaccacag ctattgtcgg agctgaaaac
    19501 aacagttccg gctggtggac tgcattctcc gattacttct ccgttcccac aggcaagtta
    19561 ttgcatctgg agtttattaa ccacggtacc ggtgttaaca actggaacaa ctggaatctt
    19621 gccgtcacta atgaaatagc cgatcgcgca gccgccgatt ataaagaata ctttgttctc
    19681 cgttcggatg cttacggctg gggtggaaaa atggcaaatg tcgatccccg ctatgctttc
    19741 gatatcaaga tgatcacaca taattatccc gataccgatg ggaatggaga tatctggaac
    19801 gacttccgca cgaccatgca aggtgcacgc gtgtcactcg atattgacca ttcggctacc
    19861 ggcaatgtat ttgtaacagc cactgccgtt ggaactaatg gtgtaacgct cgtagaaacc
    19921 taccaacaac cggtttctgc tgctgctgat ctcatggctt tcttaatttg cgatggcagc
    19981 tatttcgaaa tgaaaaaagc atacctcata ccttccaaag ttacagtagt cgaagacgtt
    20041 aatccggttt caattgctgt tacaggaact cctgcttttc ttgaattggg caacaaaaac
    20101 ttctggggtg atgcaaaagc cactgttact tttgctgacg gatcttccgc gcaagttgat
    20161 tcagccgata tatccttcaa tgttattccc gatatgacta cactcggtgt taaaacggta
    20221 gttgtagcat acagcaaaac aaaacagggc gcctactgca aagccgtaac gaccttctac
    20281 aaactggagg taaccaatcc tgttgtcggt ttggaggtta ctacaatgcc actcataacc
    20341 aaatactatt actttagcgg cgatccaatc ctgttcaaca ctacaggtct tgtggttaca
    20401 ggtacctatt ccgacggtac aaaagccgtc attccaaaag aaactcttca gtttggtaag
    20461 attccagctg cctcaggcac tcagagcgct ctcatttcgt atgtaggagc aactaaaaca
    20521 gttactacaa cttgtccgct aacgctcgta aagggtatag gtcaggtagg tgctaatgac
    20581 ttctctaccg cctggtggac ggcattctct gctgactaca aagtaccgtc aggttcaagt
    20641 aaaaccctta caatgtactg ttactccgac aacctggcaa actggcacag cccgtgtacc
    20701 attcttcgca aatcggataa gactgaaaat gcggtagttc gtatggacag cttcggttgg
    20761 ggagcaggtt atggcacagc cacactaact tctgactgga actgggataa attcacatca
    20821 aacatcagtg gctcaaaggt agtcatcacc gttacaaaca atggcaataa tactgccgat
    20881 gtactttaca atgttactta cgctaccggc gaaactcatt tccagaaata tgcgggcgta
    20941 acagttgaca gtgctgattt gaattgcgca ctcgtaacgg aggtgtccta tctcgtaatt
    21001 gttgaataat aacaaagata aaaataatta aaccatgaaa tattcatatt taatcctgat
    21061 tggattgctg gcgctcgcac cggcaactta tgccgatggc aacggaaata tcgaccagat
    21121 agcaatgaat cagactaaca atataaccgt aaaagggaaa attgtggacg ataaaggtga
    21181 accgcttatc ggtgcaaccg tacaacaaaa aggcacaacc gttggagtaa tgaccaatgc
    21241 ttcaggtaat ttctctctta ctgttccttc tgatgcttcg cttgtagtgt cctatgtggg
    21301 ttgtttaacc caggaagtat tcgtaggagg aaaaacagat atcggtatca tcacgttggc
    21361 atcggatgta aaagaactcg aacaggtggt ggtaattggt tacggaacac aacgcaaggt
    21421 tgacctcaca ggttctgttg ctattgtcaa cgctgaagaa atgaaaaaag tatcgcactc
    21481 caacatctcc acaatgctcg aaggcaaggt ggccggtgtt caaattacat ctgacggaca
    21541 accgggtgcc gaccctacag tacgtatccg tggtatcggc tcgtttggca gtacggcacc
    21601 tctgtacatt gttgatggag ttccgatggg aacaaccatt cgcgactttt ctccgaacga
    21661 tattgaaacc cttcaggtac tcaaggacgc ttctgccgct gccatctacg gctcacgtgc
    21721 cgccaacggg gtggttatca ttacgaccaa gcagggaaag aagaaccagg ccatgaaaat
    21781 agactacacc ggatacaccg gttttgacca ggtgcagaag ggtgtgtaca atgtgatgga
    21841 ttcgtaccag tatggtaaat acgtcactat ggcatacaac aatagtggta tggatgtacc
    21901 tgccggatac aatcctgcaa gtccaaaata tgttgatccg gcccaggtta acactaattg
    21961 gttcaatgaa gcattcaaaa ccggcattcg tcagaatcac aacatcaata tgtcgggtgg
    22021 tggagccaac aacacgtaca atatcggtct cgactactat agccaaaaag ggaccatggt
    22081 tggtgcaggt cctaatttcg accgtttcac agcccgtgtc aacaacgcca tggatgtgaa
    22141 atttgtcaaa atcaaaacca acatggtata tagccacagc aatcaggata acatggcgct
    22201 cagtaatgcc aacgagtacg tgcagggtct gtatggtgcc caattcccgg ttatggcttc
    22261 ggcgctgctt ttgccgccaa caatcaaagc ctacgatcca acaacctggg ttcttgacga
    22321 caaaatttcg gcagcatcag aatacagcta cgactcgtat ggttttggaa cttactacga
    22381 cgatgttcat ggcgacttac gtgcaacgaa ccttttgctc accaatagcc ttttgaaaag
    22441 aaactctgtg gttgaccgcg ttgttgcatc aggttcggca actgttgacc tcatcggtat
    22501 ggtcggtcaa aaaaccagca atcacaagct ggattacaac ctcaacctgt cgtacagcaa
    22561 gacccttgtt aaggacttca catttgtgcc tgcttttatt caaagtacaa caaattactt
    22621 gtccaaaagc aacgagaacc tgtcagaggg ctatcgcagc tatagcgatg cacttattga
    22681 aaatacactg acctacgatg gtaaatttgg gcttaatcac atcaataccg tagtgggtca
    22741 aacattccag cgcgaactct ttcacacact caccggcaag ggtgtcaacc tgcccgaacc
    22801 gtactatctg caggtaaata acgcagagga aacatcatca gcaagcaatg aaagcgaaca
    22861 cgtgctggca tcgtatatcg gtcgtctcaa ctacgatttt gatcagaaat acctcatttc
    22921 tgccaccgta cgtcgtgatg gttcaagccg tctttcttct gctgaccgtt gggattggtt
    22981 cccatccata tcggcaggct ggcgtatcga ccgtgaaagt ttcttccctg tagccaaaac
    23041 gaacatcaat ctgctcaaaa ttcgcggcag ctatggagta cttggtaatg agaatattgg
    23101 tgaataccag tacatggatg ttatgtcgag aggtaactac acttatagtt ttggaaataa
    23161 caaggttacc ggatctgcaa tatccaacta cgtaaacaca gccatccggt gggaaaagaa
    23221 gaaaacagtg gacttaggtg tcgacctcgg tatgttcaac aatcagctgg aatttacttt
    23281 cgactggtat aagtcaacat ccgaagacct gctttatagt gtggctgtac ctaccaatgc
    23341 cggtgctacc aacggaacag tgaccatgaa cgcagccacc atggaaaact caggtcttga
    23401 gttcctgctt gcatatcata accataaaca cccggttaag tttgacattt ccaccaactt
    23461 atctaccatc aacaacaagg taatcaagtt gggtgtttca ggtgaacccc gatccgatgg
    23521 ttactgccgc actgaggttg gacgtgaagt tggttcattc tatggctatg tttacgacgg
    23581 tatattccag tcgcaggctg agatagataa ccggcttaac tcagaaggcg aacacattaa
    23641 tcaaagcggt gcacagccgg gtgatgttgc ttatgccgac ctcaatctcg acggtgaaat
    23701 taccaacgaa gaccaaaaat acctgggcag cgggttgcct aagtttaact atggcttgaa
    23761 tgcacgtgct gaatggaaag ggttggacct gagcatttcg acttatggag ttgcaggttt
    23821 taaagcagtc gattttgttg acgttacttt gcacaattcg tatggcgcac tcaataaaag
    23881 tgttgatttg ctcaacgcct ggacaccgga aaacacaaat accaacgtgc cccgtgtagc
    23941 ctacaaatcc acaggttcaa tcaccaatga tatgttcagt gagcgtttca ttcaggatgc
    24001 atcctatctg aaaattgcca acatcgaatt gggatataac ttacccgaca gatggtttgg
    24061 tggctatgtt tccggccttc gtatatatgc atcggcacaa aatctggcca cattgtccaa
    24121 atacaaaggt tacaacgtag attttgccgg aggcacattt actcccggtt ataactattg
    24181 ttcgtatccc actccacgaa ccatgatgtt cggaatacat tgttcattct aaaaaatata
    24241 atctaataaa tagaatatca acatgaaaag tttaaatata attctaacaa cgttggtgct
    24301 tctgggaggc atgattgcct gcactgacca actcaatgtt cagaacccga acaatcagac
    24361 aacctacgat ttcggcaatt cagagtcgga acttcaggag gctgttatct cgtgctacaa
    24421 ccgcatacgt ctcgaaggta catttgcccg cgttggctac acactcgatg ctgtgcgcgg
    24481 cgatgaagtc tggaactcct ctcaacagtg gtatctggag tacgacaacc tcaattctcc
    24541 gggtacaatc ggtataggtg atgaatggcc atggcgcgac aactaccatg tggtaaaccg
    24601 tacaaacttt gttttatcaa aagttgacgg tgtaaaaatg tcagtagatt cgtacaatca
    24661 gattgccgga caggcccttt tcctgaggtc tctggcgtac tatcatctgg ctacctatta
    24721 ccagacagta ccattgatta ctgactatgc gtcttattcg gacatcaaca ccatgtatgc
    24781 atccaatgct actcaggatg aggtgtttga ccagatagag actgacctta aaaaagcaat
    24841 ggaaatgttg ccatcccgcg ataaaggtgg cgaatgggct aaaggccgcg ctaccagcgg
    24901 tgctgcagcc ggatatctgg cacgtgcact gatgttccgt cataaatatg cagaagctta
    24961 tgcagtactt aaagatatca tcgccggcaa atacggacat tatgaactta cagccgatta
    25021 tggcgacaat ttcagagagg gtcctgcgtt tgagaataat aaagagagcc tattcgaagt
    25081 gcaatttctg gactttggca caggaggcac cgacgaagag tggactccgg taaacataag
    25141 ctcggaagca actcagggtc acgctgtaga aagtaactac gcttcacagg aattgggtag
    25201 ctggggtgac ctggccggtt caccctggtt gtataacctg ttcaaaaaag agaacagtac
    25261 cgatggtcgt ctcgatccgc gcctttactg gacattggtt acctacgaaa atgaatataa
    25321 cacctataaa gggctgaaaa cggcagcata tcccaatggt gacccgcgca gtaacacgat
    25381 ttatcaaaaa gacattacta aaactccgct atcgaacaat gcccagggtg gaatatcaat
    25441 agcaaaattc accaatgcaa gaaataatat ctatagttcc attaccaacg gattgcattg
    25501 tggtgtcaac ctgcgtctga tgcgttacag tgatgttttg ctgcgggcag cagaatgcga
    25561 aaatgaaata agtggtccaa cccaaactgc tatcgactac attaacaccg tacgtcgtcg
    25621 tgtagctctt aaggatctta aactttccga cttcccttct gccgatgctc ttttcgaaca
    25681 aatagctaac gttgaacgtc cgaaagagtt cggttgtgaa aacggacgcg gtatcgatct
    25741 gctccgctgg ggattctttt acgatgccgg ccgtttgaat cagctggttg aacatggtta
    25801 ttacaaacgc gacggcaaag cttcaacaga agaacttact gccgaaaccg ccgacgattc
    25861 atcgttcaaa tactattata aaggacatga atatttccca atatttcaat cgacgctgaa
    25921 tgccaatccg aatctggttg gtaactcggc aaacaagaat gaagataatg gacctgcttt
    25981 caaagccaaa tggaacgttc gtcctgtagt aaaataggaa ttattgaata aaaaacggaa
    26041 acttccttat cggtatattt gttatttatt gataaggaag tttcttaaca ttaaaattac
    26101 ggacatgaga ctaaggtaca tattgatttt atctatcagc ataacagcac tatcgtttgt
    26161 tgggtgcaaa ccagatgagc ctaaacctga tgataagcca aacccctttc caactttcac
    26221 tattccaact tatgctgacg actattcatc aatatcatcg tgggcaaacc gaaacaaatg
    26281 gaatctggcc aacgtccatg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 ncatcaatat catcgtgggc aaaccgaaac aaatggaatc tggccaacgt ccatgaccca
    26461 tcagttgcat actacaaagg atattactac atgtacggca ccgatgcctc gtatggtaat
    26521 gcacacgatg gacacggaca tttccagggc aaacgttcaa ccgatttggt gaactgggac
    26581 tgggtaggcg ggccttttta tcaggcaccc acctgggtag ctgactcact caatgcgatt
    26641 cgctcgcgca tgggtctcga tgtcattgct aaagacaaaa ttgaatatgg ttactgggca
    26701 ccggttgtgc gccgggtgaa cgtgggaggt caggaaatcc tacgcatgta ctacagcatt
    26761 gttatcttta attacataaa aacaggtaat ctctcctctt caccttttga tggaagctgg
    26821 acagagcgct cttttatcgg aatgtgcgaa tcaaccgatc cggccggagc tgtatggaca
    26881 gacaaaggct ttgttaccac ttcatcatca gaccgtggaa agaacttcag tcgcgccagc
    26941 acaaatgact ggagcgcata cttctactac aacgccattg acccaactta catagtaaca
    27001 cccgagggga aacactacct cattcatggc tcgtggcata gcggttttgc tttattgcaa
    27061 ctggatgcaa caacaggaaa gcctattaat aagcttggtg aaccttatgc cagcagtgca
    27121 agtgagctta ctacgcgtta tggtgtgaga attggcacac gtacagcagg atcgcgctgg
    27181 cagggcagcg aagctcccga aatcatttac aaagatggtt actactattt gttcatggct
    27241 tacgatggcc ttgatgttcc ttataacaca cgggtggtca gaagcgcaaa catcgaaggt
    27301 ccatacctcg atatcaccgg gcgcaatttt accaacggag ctggcgacag ctacccaata
    27361 gtaacccatc cctacaaatt caatctgagc aacggatggg taggcatatc acactgtgct
    27421 gttttcaaaa aggaaaatac caacgaatgg ttttacatgt cgcagggacg attacctgcc
    27481 aacacaggtg gcaacccgta ctccaatgcc attatgatgg gacacgtgcg tcgcattgtc
    27541 tggtgtccgg cttctattaa cgaacccgac aacctgtggc caatcgcttt gcccgaacgt
    27601 tacgccgctg tacccgatta cggaaccata acgaaagatt cgctggtggg aacctgggag
    27661 cacattaacc tgaaatacag ttacgcccaa caagatctcg ccactgcgtt gaccctcaaa
    27721 gccgatggca ccatgtcggg agcgctgagt ggcaactgga gctacgatgc tacaaaaaag
    27781 caactcatcc tgggcaacat tgttgtttgt gtagaacggg aagttgactg ggaagccacc
    27841 ccacgccgtg taacatttgt atatgccggt acagagaaaa atctgaacgc aacctattgg
    27901 ggcaaaaagt caaaataata ttgaagtcag ggaaaaataa tttccacata taagttatta
    27961 tctctcaatg caataatact gtttaacaca aagtctgctc aaccttaaat tgggcagact
    28021 ttgatttgta taatcttgtt ttttacgctt tgatcttatc tttcggtcag gaaatcaata
    28081 atcggaaact tattgcctgg gtaagttatt gtcaggtgca taaacattgt ctttaacatt
    28141 gatttgtgag catcccaaat ccgcctcttt atagacataa aaaaccgact tagagtaaca
    28201 acattcgcta attccgtttt ttatgtgtta tggttttatt ggagccagaa aagtagtatt
    28261 tctgatgaca ataaccctgt atagaacccg attaaatcaa tagacaggta caaataactg
    28321 taaggctcac ttgctgaaaa caaggcatat tctataatag atatccgaga cttagtttat
    28381 gctcagccac gtgatgagcg acattagggt agtatttata aacgctgtta cgataattat
    28441 aactggattc cgacgtgata aagatatttt taccgatcaa atagccgatc cccaggcgtc
    28501 caacgctcaa acttgtattc cccacatcgc catgaacact tgagttgaat tccttaaaat
    28561 cattcggtcc atatcccacc cacgagtaaa tgcgataatc ttcagtattg agctgaattt
    28621 gcaatttttc atataagttg aacttcaaat tcagcttggt actgaatccg ctacccatat
    28681 tgtaatccct ttcatcatac ctgtaatggt ctgtttgact tcctcccagc aaaatggcgc
    28741 taaggtatgc cgaagctgtg aaagttactt tgttttttgc agatatctta tacaaaagtc
    28801 cgggacctgc cgatgctgct tcagaaattt tataggggta aagctgcacg tgattggcgt
    28861 cggattgcga ttcgtagtaa ttaaaatgct ggaaaaggcc ataattaagg cattgattct
    28921 gcgatttaag cgacacattc gtagaaaaaa gcatacccaa aacatttact ctgcttacaa
    28981 ttggctgtgt ggagaaaaag tttccaccaa gtttaaatgc aaagtagtcg tatggttttt
    29041 cattttcctt atcaaattcg ttgccgtaaa tgagtcctaa atcatagcta agcatattgc
    29101 tgtaatcctg tcgt
//
LOCUS       DAOPZK010000009        27204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1745_length_27204_cov_22.097167, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000009 DAOPZK010000000
VERSION     DAOPZK010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 27204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27204
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1745_length_27204_cov_22.097167"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..254)
                     /locus_tag="PKH68_01040"
     CDS             complement(123..254)
                     /locus_tag="PKH68_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11549.1"
                     /translation="MADFPAEKVQPDEKYFFELVKRVTSSCKGNYIAILDSCNKYKH"
     gene            1377..2298
                     /locus_tag="PKH68_01045"
                     /pseudo
     CDS             1377..2298
                     /locus_tag="PKH68_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
     gene            2380..3363
                     /locus_tag="PKH68_01050"
     CDS             2380..3363
                     /locus_tag="PKH68_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNX11550.1"
                     /translation="MNNFDNTDNKEQRINKPPMGGGGALTDTILMIEPVAFGYNIETA
                     VNNFFQQKENHQSEDIAGIAHAQFSNMVKLLQNSGIKVIVVKDTEYPPTPDSIFPNNW
                     ISFHKPSKVVLYPMFAPNRRQERRMDVILTVENETGKTYDLLDYSDYELRNIFLEGTG
                     SIVLDRINRIAYASLSQRTDKQLFEKYCKEMLFQPVSFHATQQVGQERLPIYHTNVMM
                     CVGEELAVVCPETIQDENERHRLMKMLNETGKKIVEITEEQMHCFAGNMLQLKNKNGE
                     KFMLMSLSAFNSLNNIQLEALKAENELIVPDITIIEKYGGGSVRCMVAEVF"
     gene            3443..4684
                     /gene="rocD"
                     /locus_tag="PKH68_01055"
     CDS             3443..4684
                     /gene="rocD"
                     /locus_tag="PKH68_01055"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNX11551.1"
                     /translation="MNNTKKYSAEELMELENRYGAHNYHPLPVVLEKGEGVYLWDVNG
                     KKYYDFLSAYSAVNQGHCHPKIVETLTSQAQKLTLTSRAFYNDKLGVYEKFVTEYFGY
                     EKVLPMNSGAEAVETALKLCRKWAYQKKKIAENDAQIVVCAENFHGRTTTIVSFSVDT
                     DAYSDYGPFTPGFIIIPYNDLEALHNVLNENKNVAGFLVEPIQGEAGAKVPDDGYLRQ
                     TYELCKENDVLFIADEVQTGIARTGRLLACEYEDVKPDILILGKALSGGVYPVSAVLS
                     SSEIMDIIKPGQHGSTFGGNPLAAAVAVAALEVVRDERLAEKAYELGLIFRDEMSEFC
                     KTSNVAELVRGKGLMNALIINNADHKNLAWDICLKMAENGLLAKPTHTNIIRFAPPLV
                     ITAEQLMECIGIIKASVNAFE"
     gene            5120..6730
                     /locus_tag="PKH68_01060"
     CDS             5120..6730
                     /locus_tag="PKH68_01060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNX11552.1"
                     /translation="MKETKYIFVTGGVSSSLGKGIIAASLGKLLQARGFRVTNQKFDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNIETHRANNITTGRIYQNVIN
                     KERRGDFLGKTVQVIPHITDEIKRNVKILGNKGDYDFVITEIGGTVGDIESLPFIESV
                     RQLKWELGKNCLIIHLTYVPFLAAAKELKTKPTQHSVKMLQEQGVQPDILVLRTEHDI
                     PMELRRKVALFCNVEPTAVMQSIDASTIYRVPLNMQKEGLDKVALTKMGFDLTKLPEP
                     DLEKWIGFVEQMEGATEVVNIALVGKYVQLQDAYKSITESLLHASAYSNRKLKLDMIL
                     SDKITPENVEQKLGKKDGIIIAPGFGQRGMEGKFTALQFAREHNVPCLGICMGMQTMC
                     IEFARNVLGYDDANSTEIDPHTTHNVVDIMDEQKNILNLGGSMRLGAYKCKLKKGSKA
                     YQIYGKENISERHRHRFEFNNQYKDEFEKAGMSCSGLNEESDLVEIVELKNHKWFVGV
                     QFHPEYSSTVVNPHPLFYSFIKAAIDKK"
     gene            6879..8795
                     /gene="yidC"
                     /locus_tag="PKH68_01065"
     CDS             6879..8795
                     /gene="yidC"
                     /locus_tag="PKH68_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNX11553.1"
                     /translation="MDKNTIIGFILIAAIVFGFAALNKPSEAEIAKRKKYNDSIALVQ
                     QQQAVIQQQADATKLNSANPDSTALNDSAAVALKSDAFGAFKTAAFGEEKFFTVENEL
                     IRLKFSNKGGRIYSAELKNYRTHDSLPLILFDKDESALGFTLTTTNNRVVNTADLYFE
                     PVGEVTKDAKGNQTFVFRLKTDQPGSYLDFIYTIPANDYMVKYDIKATNMNAIIPPSM
                     NLLEMNWSAKIRQQEKGRQFEERYSALHFKYLTDDVNKLSESKNDQEDPANKVKWIAF
                     KDQFFSTILIADESFVSTSLTSEMMPEKSGYLKSFSSKMAVGFDPAGNQPTGFRFFFG
                     PNRYKILSGYDKGVPGDEKLYLRDLIPLGWTIFGWVNRFMIIPMFNLFSTFIGSFGLI
                     ILLMTVVIKIVLFPMTYKSYVSSAKMRVLKPEIDEINARIPADKAMERQKATMELYNK
                     VGVSPMSGCLPMLLQMPILFAMFSFFPSAIELRQQSFLWAHDLSAYDSILSWNAYIPL
                     VTPYFGNHISLFCLLMTISSIISTKLTMSQTPTASDQPGAGMMKWMMYLMPLMFMFMF
                     NNYASGLSYYYFLSTLITIGQTYIIRATVDDKKLLAQLHAKGSKAKPKNKSGFMERLE
                     KMQREQQKALKNRK"
     gene            8898..9908
                     /locus_tag="PKH68_01070"
     CDS             8898..9908
                     /locus_tag="PKH68_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11554.1"
                     /translation="MKNVNYLILLVFTLFFLSSCTKKNRFEIDTKKEKVEVTIHRFDR
                     DLIHLDLNHVRQSVDSLYRTYPGFLPLYIYNVLDTVPTDTLAVADMFHQFVQDTAIQS
                     VNKKVMETFADVSDIEKDISGGFTYIHHYFPQVPLPEVYFFVSGFNRSVLMTDSLLGV
                     GTDFYLGAGYPPYKSFIYEYLLQNMRRENLAIDVVSATLFRYFTFDGVNNALIDNMLH
                     RGKVIWLLSVFMPDKKMEDIMGYTPAQWHWAETNEEQVWKTIVAQKHLFSTDMQLINK
                     YLNDAPFTAPVSQESPGRLGTWIGWRIIDSYMEQNKTMTLEQLMKENNYRKILEQSHY
                     RP"
     gene            10121..11080
                     /locus_tag="PKH68_01075"
     CDS             10121..11080
                     /locus_tag="PKH68_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNX11555.1"
                     /translation="MSSRLKEYLSVDNYIFPALDVPFDVYPSRLYNRKSLYAAFDYHH
                     PETYYLTHDKIVYDYYRKIGNETRNIKETLARALHDHSIKDEMNEFLDQFEERKIVAV
                     MGGHKLGRDEKVFIQLAQLSKQLTEKGFLMITGGGPGAMEATHLGAWFAGKTNDELND
                     AVRILSVAPLYDHPEWLSTAIHVMERYPETGYKSLGIPTWLYGHELSTPFATHIAKFF
                     ENSLREEGLLAVAKGGVIFAPGSAGTMQEIFQDLAQNHYESYGYASPMIFLNQTYWTD
                     DRPIYPLIQKMIENRDLKNIDLSLYDENSDIIAHLEKFVAENQ"
     gene            11137..11829
                     /gene="radC"
                     /locus_tag="PKH68_01080"
     CDS             11137..11829
                     /gene="radC"
                     /locus_tag="PKH68_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNX11556.1"
                     /translation="MEENYLSIKNWAEDDRPREKLLNKGASVLSDAELLAIMIGSGSR
                     NESAVQLCRKILKASHDNLNELARLSIDELCKFKGIGEAKAITISAALELGNRRKTNE
                     VRERARITQSRDLFELFEPILLDLKHEEFWVALLNGANKVLDVKRLTQGGTRQTVVDI
                     PMILKMSLEKSAPAIAVAHNHPSGQDNPSREDDAITQRIKKGCEAIDIRLLDHIIIAG
                     GKYYSFADEGKI"
     gene            11922..12836
                     /locus_tag="PKH68_01085"
     CDS             11922..12836
                     /locus_tag="PKH68_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX11557.1"
                     /translation="MNLLEINNVVKQYSGQLALNDVSLIVPRGSVYGLLGPNGAGKTT
                     LIRIINCITAPDSGEILLNGKKLTPADVRNIGYLPEERGLYKKMKVGEQAMYLARLKG
                     MTKHDATKALKYWFEKFEIQDWWNKKVEELSKGMAQKVQFITTILHNPDLLIFDEPFS
                     GFDPVNADILKKEILALRDKGATIIFSTHNMESVEELCENISLVNKARIVLQGKVADI
                     RASQSTRSLVIRSSTPFTPYNCNIISQTDKNGMFETTLVKGSNLSNNELLAELMKQTD
                     IVSFEELLPTMNDIFIRTVTTQPPKGGV"
     gene            12942..14273
                     /locus_tag="PKH68_01090"
     CDS             12942..14273
                     /locus_tag="PKH68_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX11558.1"
                     /translation="MSKIGIIISREYTTRVMKKSFLLLTFLTPLLFVAMIMIPLWLSN
                     IQDDTLKNIIVIDKTGLYHDALKSNDTYTFVFSDKPLDNFRKKEKTGNELTAVLYITA
                     DLSTKPNAVSLYSEKQVNVELKSYISGLLGDFVQKQKLAGYNIPNLKEVVEKSKTNVD
                     ISTIKWNADGQEKEGSAELALIIGMISAFLIYIFIMSYGSQVMSGVLQEKTSRIVEVI
                     ISSVKPFELMMGKIIGIALVGLTQFFMWIALTLIITAGLSVFMGGGIDTEAIQSAQQI
                     NMPGMQSGMSQSDTQSMITEIYKSLNGFDFFKTLVLFIIYFLGGYLLYASLFAAIGSA
                     VDNETDTQQFSMPLTIPILFAIYAAIYSAQNPDGPLAFWCSLIPFTSPIVMMVRLPFD
                     VPAWQIVLSIVILVLSFIGTTWIAGKIYRTGILMYGKKVSWKELWKWVRYK"
     gene            complement(14319..15647)
                     /gene="creD"
                     /locus_tag="PKH68_01095"
     CDS             complement(14319..15647)
                     /gene="creD"
                     /locus_tag="PKH68_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HNX11559.1"
                     /translation="METTNEKIEKFSQSVTLKAIIIGVLTLLMLIPGSMIQELIHERQ
                     NRSRETIEKINSKWSNAQTITGPVLVIPYKITTTDEKKNSKTENHTFNLAPESLIIKS
                     KLTPEERHYGIYKSILYKSSLDISGNFKAIDQKQIEGEPQWGDAYIRLGLSDLRGITK
                     NIDFEINKQHFTAETGGSDDAIGQGLIISLKNAVLFQPEKRLDFHCNMDLNGSSNLNF
                     IPVGRSTQVFVEGNWKAPGFIGNFTPQYKINETGFTARWNILHFNRNIPESWKDGNII
                     DTADNSFGVNLVDTVDHYQLNMRSNKYALMFIALTFVVFFFVEVLTRKRIHPIQYLLV
                     GVALILFYSLLLSISEQLNFGISYLIASIATIGLITVYANSIFKNKTQTGILAFLLGI
                     LYVFLYVVLQLEDVALLIGSIGLFVILGIIMYLSHKIKWYNEDTRPKMKQ"
     gene            complement(15719..16009)
                     /locus_tag="PKH68_01100"
     CDS             complement(15719..16009)
                     /locus_tag="PKH68_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX11560.1"
                     /translation="MANIIANLNKAFDSRIRLGIMSVLIVNDTVDFNTLKELLELTDG
                     NLASHIKALENLGYIQTIKQFISRKPNTRYKITDQGRTSFQDHLDALSNLIG"
     gene            complement(16012..16614)
                     /locus_tag="PKH68_01105"
     CDS             complement(16012..16614)
                     /locus_tag="PKH68_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11561.1"
                     /translation="MEKHTEDLKAIREMMEKSSKFLSLSGLSGIMAGVTAIAGALFAY
                     LYLLRHHSSAEFLLKQDLLILTVDAILVLVISLGFAIYFSRRKAKKKNQKLLSTVVLN
                     TMYNLGIPLAAGGILSIIMISRGQTELVAATTLIFYGLALVNVSKFTYGEVHYLGIAE
                     IIIGLLAAVFIHSGLILWTIGFGICHVVYGFIMYKKYDLN"
     gene            complement(16791..16949)
                     /gene="rpmH"
                     /locus_tag="PKH68_01110"
     CDS             complement(16791..16949)
                     /gene="rpmH"
                     /locus_tag="PKH68_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNX11562.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMATANGRRVLAARRAKGRAKLTV
                     ADEGRHKF"
     gene            complement(17101..17847)
                     /gene="fabG"
                     /locus_tag="PKH68_01115"
     CDS             complement(17101..17847)
                     /gene="fabG"
                     /locus_tag="PKH68_01115"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNX11563.1"
                     /translation="MKLLEGKVAIVTGAARGIGKAIALKFASEGCHVAFTDLVIDENG
                     LATQRELEALGVKAKGYASNAASFEATHQVVDEIVKDFGRVDILVNNAGITKDGLMMR
                     MTEQQWDAVINVNLKSAFNFVHACTPVMMKQKSGSIINMSSVVGVSGNAGQTNYSASK
                     AGMIGLAKSIAKELGTRGIRANAIAPGFIETEMTHALTEEQRAKWAETIPLRRGGSPD
                     EVAKVCVFLASDLSSYVSGQVINVCGGMNT"
     gene            complement(17983..19092)
                     /locus_tag="PKH68_01120"
     CDS             complement(17983..19092)
                     /locus_tag="PKH68_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11564.1"
                     /translation="MKQSFKITMLLMSVAMLFSACKKNPDVNKSNFNFIKAEANYLGN
                     IYRNNVNAFEAYFFDSGLGTDANGYITGSGAFLFLDMNTTKNATSIPSGTYKVNNENV
                     NESVELTFQRGKMVLNNNNEDVPSGSYIAIVNNGKITDYLFLSSGSIIVNKMGNYSIT
                     GQVTTSDNEFYEFSFSGSIPTTDLVTPYPQTLKKGQLWYWGDIDQVGLNIYTIWLADQ
                     NINMTNMSGNGDLMRIEVYTPTSYTTSLPNGTYPVKINTVENQTVIDGFNNGTEDLGT
                     WYYTADAFPITGGSMTVVKNAGSSYNLNFTFIDELGNTISGTYNGDLGLVNKASTAQG
                     IKSRIQKVKTNRAVATETDQYNIRIHRGSRINSAK"
     gene            complement(19245..19502)
                     /locus_tag="PKH68_01125"
     CDS             complement(19245..19502)
                     /locus_tag="PKH68_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNX11565.1"
                     /translation="MKSGIHPENYRPVAFRDMSNGDTFITRSTVNTKETVDIDGATYP
                     LVKLEISSSSHPFYTGKSKLVDTAGRVDKFMNRYGNRGEKK"
     gene            complement(19670..20431)
                     /gene="truA"
                     /locus_tag="PKH68_01130"
     CDS             complement(19670..20431)
                     /gene="truA"
                     /locus_tag="PKH68_01130"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNX11566.1"
                     /translation="MKRRYFIRFAYDGTAYCGWQVQPNGLSVQEVLTKALCTILRTED
                     IEIVAAGRTDAGVHARNMVAHFDVEDRLPAGLDLVNKLNSLLPSDISVIEIREVKPEV
                     HARFDALSRRYEYHLVLQKDVFRHPFAMRIYNPLDFDRMNEAAKILFEYTDFTSFSKL
                     HTDVKTNNCKIMAARWEQREEEWIFIVEADRFLRNMVRAIVGTLLEVGRGKMTIDEFR
                     QVIDAKNRCNAGSSVPAKGLFLVEVAYPENIYTGD"
     gene            complement(20515..21387)
                     /locus_tag="PKH68_01135"
     CDS             complement(20515..21387)
                     /locus_tag="PKH68_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11567.1"
                     /translation="MKNRWFFLFSVLICLTSSLKAQDTIVLKNSSQIIAKISEIGPGG
                     IKYKLQENPDGPDYILEKFKVHEIRFKNGFVQQYDVTEELPVEQEIEYINDVPVRKQI
                     EIREKRIINDTRVAEQSGPPVRMGQKFNQNKLRYNSALYIPQPGDPYNPSVAGIASFF
                     IPGLGQMLSGEVGRGLGFLGGYMGTSFICMTGMAIAQGDYSYDYDSEFNHSYNRNNAE
                     LGFGLFIVGGVAMLGTYIWSIVDAVKVAKVNNMYMQDLRKDQVSIQLKPYMETKKYPG
                     ITDRPVGMTLQINF"
     gene            complement(21465..22484)
                     /gene="pheS"
                     /locus_tag="PKH68_01140"
     CDS             complement(21465..22484)
                     /gene="pheS"
                     /locus_tag="PKH68_01140"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNX11568.1"
                     /translation="MKNKIQQLMDEINSLRVSTLDEAEALRIKYLSKKGEITSLFNDF
                     RNVANEDKREVGQLLNELKNKAQDKLNELKESLSTSGVSEQKTDLTRTAEPVKLGTRH
                     PLSLVKQEIADIFSRIGFTIAEGPEIENDWNVFGALNFPPEHPARDMQDTFFIEKNPD
                     ILLRTHTSSVQTHVMSSQKPPIRMIFPGRVYRNEAISYRAHCFFHQVEGLYIDKNVSF
                     ADLKQALLYFAKEMFGADTQIRLRPSYFPFTEPSAEMDISCNLCGGKGCAFCKHTGWV
                     EILGCGMVDPNVLENCGIDSKVYSGYAFGMGVERITNLKYQVKDLRLFSENDVRFLGE
                     FTGAF"
     gene            22689..23357
                     /locus_tag="PKH68_01145"
     CDS             22689..23357
                     /locus_tag="PKH68_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNX11569.1"
                     /translation="MKNILDFLSELKNHNDRVWFSEHKSEYDTARIDFEQICSSLILK
                     IAAFDEDIRLVDVRDCVFRIYRDIRFSPDKTPYKTHFGAFIASAGGRKSDRGGYYLHL
                     DPAGCFFSIGVWSPTPVVLKAIRRSIYDNIDEFNEIRNNPDFRTLFNDSFYEEDKLKT
                     VPQGFSKDFPDAELLKLKHFMVTYDIDDNMLNSSEFVEKLAEIAEKSYPFLQFLNYSV
                     DEVI"
     gene            23389..25398
                     /locus_tag="PKH68_01150"
     CDS             23389..25398
                     /locus_tag="PKH68_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNX11570.1"
                     /translation="MKSSKMLPIVASDPWLEPYAAAIQGRYDYYLSVEKRLTGEKGSL
                     SDFATGYLYFGLHHTPDGWIFREWAPNATAIFLIGDFNNWQKNKEYQLTKLENGVWEI
                     QLPEDAMKHLQLYKLIVEWEGGSGERIPAWARRVIQDEHTKIFSAQVWNPENPFQFKV
                     DKFVPDTKPLLIYECHIGMSSSEEKVSTYDEFRRTVLPRIVNDGYNCIQIMAIQEHPY
                     YGSFGYHVSSFFAASSRFGTPEELKRLIDQAHEMGVAVIMDIVHSHAVKNEVEGLGRF
                     DGTPYQYFHGGERREHPAWDSLCFDYAKPSVLHFLLSNCKFWLEEYKFDGFRFDGVTS
                     MLYRSHGLGEDFNGYGSYYNLNQDGDAICYLTLVNKLIHEVNPHAITIAEEVSGIPGL
                     ASKISDGGFGFDYRMAMGIPDFWIKYIKEVKDEDWKVGHIYWELTNRRADERTISYAE
                     SHDQALVGDKTIIFRLIDADMYWHMQKGDQNFRVERGIALHKMIRLVTSTTINGGYLN
                     FMGNEFGHPEWIDFPREGNGWSYKYARRQWELVDNPDYIYHGLGEFDRAMIRLIHSVK
                     HFNETPLYQLWDNEGDQVLAYQRNDLVFVFNFHGTNSYTDYGILAEPGSYKVVLNTDN
                     PLFDGHGLIDESIEHFTQPAPEEYMGKQWLKLYIPARSAFVLKKQ"
     gene            25532..26002
                     /locus_tag="PKH68_01155"
     CDS             25532..26002
                     /locus_tag="PKH68_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11571.1"
                     /translation="MMDEIQLNEPKKDYNEPMHTAEHILNQTMVRMFNCGRSKNAHIE
                     RKKSKCDYLLSQAPSAEQINEIEHRVNEVIQQNLPVTEEFLTYKEAAAFVDLSKLPDD
                     VSETLRVIKVGDYDACACIGQHVKNTSEIGHFEILTHDFENGRWRVRWRVTAIN"
     gene            26008..26694
                     /gene="mtgA"
                     /locus_tag="PKH68_01160"
     CDS             26008..26694
                     /gene="mtgA"
                     /locus_tag="PKH68_01160"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNX11572.1"
                     /translation="MAMKKLAKLLRNLVILFFASSILAVLVLRFVPVYITPLMLIRSV
                     EAMVDGETPKIKKDWVPLNKISRNMVQAVVASEDNLFMEHNGFSFRDIEKALEHNKKG
                     KRIRGGSTISQQTAKNVFLWPQRSWVRKGLETYFTVLIEFLWTKERIMEVYLNVIETG
                     NGIYGVQAASRKYFGVNASNLTRSQAALIAVCLPNPRKFSPANPSGYISRRKNQIVNL
                     MGKLKRVDFE"
     gene            26955..>27204
                     /locus_tag="PKH68_01165"
     CDS             26955..>27204
                     /locus_tag="PKH68_01165"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443692.1"
                     /note="lipoyl/octanoyltransferase; catalyzes the transfer
                     of the lipoyl/octanoyl moiety of lipoyl/octanoyl-ACP onto
                     lipoate-dependent enzymes like pyruvate dehydrogenase and
                     the glycine cleavage system H protein; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNX11573.1"
                     /translation="MKYDDWGLIEYKQAWEKQEKLFTETLNRKAEGLQTENHLIFCEH
                     PHVYTLGRSGDENNMLLDYIQLQAKNASFVHTNRGGDIT"
BASE COUNT         8133 a         5521 c         5635 g         7915 t
ORIGIN      
        1 aaaatgcatg ctaaacaatt taatctatga aatattattt tattattttt cacttagtgg
       61 gttttttcat ccaataatca gagcacacaa tgcatttttg ttaaatagtt ttccaatacc
      121 gtttaatgct tatatttatt acagctatct aaaatggcaa tatagtttcc tttgcaagat
      181 gatgtcaccc gctttacaag ttcaaaaaaa tatttttcat ccggttgaac tttttcggca
      241 ggaaaatctg ccataatttc cactttttga tttggtgtaa atatctgcca ttttgtcagt
      301 ctggtttttt tttcctgctg ttgctggaaa cattttcagt atatatttgc tatttgtcag
      361 ttattcacac tattgcgtgc gcgcagaaac actttgtcgg accgacaaaa tataattgcg
      421 tctgcgcaga gatattttgt cagaacaaca atatatctct gcgtcaactc agaggccttt
      481 ttcaccccct aaattgaccc tttgtgtcgg cgcagaggta gttttccgga ctaaaagcat
      541 gccattttgt ccgaaaagag gtactttgta ctcaaaaata acccatttta tacgcgcaga
      601 gatactttgc caaaggaaca aagtgtctct gcttcaaagc agaggtatat tgtcagagca
      661 acaaagtgtc tctgctacaa agcagagata ttttgtcgaa gcagcaaagt acttctgcta
      721 caaagcagag atatgttgtc ggagcaacaa aacacttctg cgtttgcgca gagatatgct
      781 gtcggtaata atatggttaa ttcagtatta cgaaatactt catcctaatg tcgcctctac
      841 gaggcttatt atttagggtt taatgctgat gttctaccat aatatcgcct ctacgaggct
      901 tattcattgc tgaacataag ctatctgatt tcagcttaat ttttactctt caactccgta
      961 ggagcgacat tatggtagaa atgatgttat gaacaaacac gaaagctccg caggagcgat
     1021 attatgttag atatctgaag tgatgaatat ttaatttacc cgaatggtct tcacatcccc
     1081 tttaagggca tggagcggta attattctaa cataagactt tacctctggt gctgaatatg
     1141 cagcctcgta gaggcggcat tataaaaagt caagacgact tcagcgagaa tctatgtttt
     1201 ctattgtttc taaatatgtt agaatgacta cattgaaaag cttttaaaat ccggtgcaca
     1261 aacgccggat ttttttattt tgaaaaataa accaaataaa tatgcaaaaa ataaacaatt
     1321 catattatat ttacattcta ataataaacg gttttttacc attagtaccg gaatgtatga
     1381 atcttcatat tgtcaacgaa acatcgaaac taaaagcagt tgtgttgggt cagcccggct
     1441 ccctcggtcc tgtacccacg cttgaaatga cctacgaccc gaaatcgtat gagtcggtaa
     1501 agaaaggaat ttatccaacc gaagaagccg ttttcaaaga gatgagtgct tttgagaaag
     1561 tattgctcaa atatgatgtg caggtgttcc gaccctggat actcgaaaac tgcaatcagg
     1621 tcttcgcacg cgatgtagct attgttatcg acgataaaat aataaattcc aacatcatcc
     1681 ccgaccgtga agatgaaaag gaagcatacg aggtaatcta tgacagcatc gcttacaaca
     1741 agatttataa tttacccgaa aaagcacacg tagaaggtgg cgacgtggtg ttatacaatg
     1801 atattatttt catgggtgta tataccggaa tggattatgc aaaattaaaa tctgcacgaa
     1861 ctaacgcata tactgtacat ttctttcgtg aactttttcc tgagaaaaca attattccgc
     1921 ttgaactgat aaagaatgat accaaccctt acgaatcggt gctgcatctc gattgcactt
     1981 tcatgccggt aaacgagcac aaggcaattg tttacaaaca tggatttaag aacataaaag
     2041 attataatat cattctggat gttttcggag aacaaaaata tatttgaaat aacacaggag
     2101 gaaatgtatc ggatgaatac caatatattt tcaatctcgc ccgataccgt tgtttccgaa
     2161 cagaatttca cccgcctcaa caatcatctc gaaaatgaat ggggcatgac agttgaacgg
     2221 gtaccctatg ccgaaatatc caaaatggga ggcttgctcc gttgttccac attgccactg
     2281 gtacgggaag ccccctaaat cccccgaaag ggggacttaa aaaccagttt cgacagagca
     2341 gaaatataat tacattgaaa aatatattat tgaaatatta tgaacaattt cgataacaca
     2401 gataataaag aacaacgcat taataagccc cctatggggg gtgggggggc tctcaccgat
     2461 accattttaa tgatagagcc ggttgcattc ggatataata tcgaaacagc cgtcaataat
     2521 tttttccagc agaaggaaaa ccatcagtcc gaagacattg cagggattgc tcatgcgcaa
     2581 ttttcgaaca tggttaagct cttgcagaac tccgggataa aagtaatcgt agtaaaggac
     2641 acagaatatc cacctacacc tgactccatt tttcctaata actggatttc gtttcataaa
     2701 ccatccaaag tagtacttta tccgatgttc gcgccaaacc gcagacagga acgccggatg
     2761 gatgtcatac tcaccgtaga aaatgaaaca ggtaaaacct atgatctgct cgattattct
     2821 gattacgaac tgcggaatat ttttctcgaa ggaacaggca gtattgtgtt ggaccgaatc
     2881 aacaggatag cttacgcttc actttcgcaa cggacagata aacagttgtt tgaaaaatat
     2941 tgtaaagaaa tgttattcca gcccgtaagt tttcatgcaa ctcaacaggt aggtcaggag
     3001 cgtttaccca tttatcacac caatgtaatg atgtgcgtgg gtgaggaact ggcagttgta
     3061 tgtcccgaaa ccattcagga tgagaacgaa agacacagac taatgaaaat gcttaatgaa
     3121 accggaaaaa aaattgtaga aatcaccgaa gaacagatgc attgctttgc cggcaatatg
     3181 cttcagttga aaaataaaaa cggagaaaaa tttatgctga tgtcgctcag tgccttcaat
     3241 tcactgaata atatacaact ggaagcgctg aaagctgaaa acgaactcat cgttcccgat
     3301 attaccatta tcgagaaata tggcggcgga agcgtgcggt gtatggtggc ggaagtgttt
     3361 taatgattaa aagaacacat atatcacata aaactaagag tttatttcaa ataaatctgt
     3421 aaattttaaa taagttaata tcatgaacaa cacaaaaaaa tactcagctg aagaattgat
     3481 ggaacttgag aaccgttatg gcgcacataa ttatcatccg ctgccggttg tgctcgaaaa
     3541 aggggagggc gtgtaccttt gggatgtgaa cgggaagaag tattacgatt ttctttcggc
     3601 ttattcagct gtaaatcagg gacactgtca tcccaagatc gtggaaacgc tgacttcgca
     3661 ggctcagaaa ctgacactta cttcgcgcgc attctacaac gacaagctgg gagtgtacga
     3721 aaaatttgta acagagtatt tcggttatga aaaggtgctt ccgatgaaca gcggtgccga
     3781 agctgtggaa accgcgctga aactttgtcg taaatgggcc tatcaaaaaa agaaaattgc
     3841 tgaaaatgat gctcagatag tggtttgtgc cgaaaatttc cacggccgca ccaccaccat
     3901 agtttccttc tcggttgata cggacgctta cagcgactac ggacccttta cacccggttt
     3961 tatcatcatc ccttacaacg atttggaggc gctccacaac gtgctgaatg aaaataagaa
     4021 tgtagccgga tttctggttg aacccattca gggcgaagcc ggtgcaaagg tgcccgacga
     4081 tggatattta cggcaaacct acgaactttg taaggagaat gatgtattat tcattgccga
     4141 cgaagttcag acggggattg cacgaaccgg caggttgctg gcttgtgagt acgaagatgt
     4201 caaacccgat attttgatct tgggcaaagc attatcgggt ggagtgtatc cggtttcggc
     4261 agtgctttcg agtagtgaaa tcatggatat catcaagccc ggacagcatg gttctacatt
     4321 tggcgggaat ccgctggcag ctgccgtggc tgtagccgct ctcgaagtgg ttcgtgatga
     4381 acggctggct gagaaggcgt atgaattagg gctgattttc agagatgaga tgagcgaatt
     4441 ctgcaaaaca tccaacgtgg ctgaactcgt tcgtggaaaa ggactgatga atgcattgat
     4501 aatcaataat gcagatcata aaaacctggc ctgggacatt tgtttgaaaa tggccgaaaa
     4561 cggactgctc gccaaaccaa cccataccaa tatcatccgg tttgcaccac cgctggtgat
     4621 tactgcggag caattgatgg agtgtatcgg tattatcaag gcgtcagtca atgcttttga
     4681 ataaataatc tctttacaga aactaataat cagggcggat caacaacaat ccgccttaat
     4741 tatgcgtagc ggtttttggc ttctgtcgaa gcgtaaaacg tctgtctttt gttacatgtt
     4801 tttatttatc tcgctcttaa taaaggcagg ttaagtaccg ctgctgcatc aggggtgatg
     4861 ttgcaaacaa cagtcgggca gcggtaccag caaaaaaaaa gttgcctgtc attccgaacg
     4921 taacaccgtg aagtgaggaa tctgaatagc atttaataag ttattgttag tatagcgaag
     4981 gttatggagt agtttgtgcg acaacagtcg ttaataatac gaaattcaac ccaccgcttt
     5041 atttattcag ttttttttcc gaaatttgca gacttaaaat tcgatttttt cagaagtcat
     5101 ttttaagtaa acattcaacg tgaaagagac caagtacatt tttgtaaccg gcggtgtatc
     5161 atcgtcatta ggtaaaggga ttatagctgc ttcgctgggc aaactgcttc aggcacgcgg
     5221 cttcagggtg actaaccaaa aatttgatcc atacatcaat atcgatcccg gaacattgaa
     5281 cccatatgaa catggtgaat gttacgtgac ggtggatggg cacgaggccg atctggatct
     5341 cggacactac gaacgatttc tcaacattga aactcaccgt gccaataata tcactaccgg
     5401 acggatttat cagaatgtga taaacaaaga acgcaggggc gacttcctgg gtaaaaccgt
     5461 gcaggtaatc ccgcatatta ccgacgaaat caaacggaat gttaagattt tgggcaataa
     5521 aggcgattat gactttgtaa ttactgagat tggaggaacg gttggcgata ttgaatcgtt
     5581 gccgttcatt gagagtgtac gtcagttgaa gtgggaactg ggtaaaaact gtctgattat
     5641 tcatctgacc tacgttccgt ttctggctgc ggctaaggaa ctgaaaacca aaccgaccca
     5701 acattcggtg aaaatgcttc aggaacaagg cgtgcaaccg gatattcttg tcctgcgtac
     5761 cgaacacgat atacccatgg aattgcgccg caaagtggcc ctgttttgca atgtggaacc
     5821 tacggctgta atgcagtcaa ttgacgcttc aaccatctat cgtgtaccgt tgaatatgca
     5881 gaaagaaggt cttgacaagg tggcattgac taaaatggga tttgatctca caaaactgcc
     5941 tgaacccgat ctggaaaagt ggataggatt tgttgagcaa atggaagggg caaccgaggt
     6001 ggtcaacata gcattggtag gcaagtacgt acaattgcag gatgcatata aatcaatcac
     6061 tgagtcgctg ctgcatgcca gcgcgtattc caaccggaaa ctgaaactgg atatgatcct
     6121 gtccgataag attactcccg aaaatgtgga gcagaaactc ggaaaaaagg atggtatcat
     6181 catagcaccg ggatttggtc aacgcggaat ggaaggtaaa ttcacagcat tacagtttgc
     6241 acgcgaacac aatgtccctt gtttgggaat ttgtatggga atgcaaacca tgtgtattga
     6301 gtttgcccgc aatgtacttg gttatgacga tgccaacagc accgaaattg acccgcacac
     6361 cactcacaat gtagttgata taatggatga gcaaaaaaac atactgaacc tgggtggaag
     6421 tatgcgtctg ggagcttaca aatgcaaatt aaagaaagga agcaaagcgt atcagatata
     6481 tggaaaagaa aatatcagcg aacgtcaccg tcaccggttc gagttcaaca atcagtataa
     6541 agatgaattt gaaaaagcag gaatgagctg cagcggtctt aacgaagaat ctgatctggt
     6601 ggaaatagta gaacttaaaa atcacaaatg gttcgtagga gtacagtttc atccggagta
     6661 cagcagcaca gtggtcaatc cacatcccct tttttacagt tttattaaag cagcgattga
     6721 taagaaataa gttacaagtt ataagtttca agttacaagt tataagttac aagataatta
     6781 gagtataaac aaaagtaaaa ttccggagag ttctcccaca tgctttcacc ctgtaaatag
     6841 caatcgaaga ggcactctga atttgtaaac aatcagaaat ggataagaac acaataatcg
     6901 gttttatttt aattgcagcc atagtttttg gttttgcagc gttgaacaag ccttcggagg
     6961 ccgaaatagc aaaacgaaaa aagtacaatg attcaattgc gttggttcaa cagcagcagg
     7021 ctgtcattca gcaacaggct gatgcaacaa aacttaattc agccaacccg gacagtacag
     7081 cactaaacga ttcggctgcc gtagcactca agtccgatgc tttcggagct ttcaaaacag
     7141 ctgcatttgg cgaagaaaaa ttctttacgg ttgaaaatga actcattcga ctgaaattca
     7201 gcaataaagg aggacggatt tattctgccg aactgaaaaa ctaccgtaca cacgactcgc
     7261 tgcctcttat tcttttcgac aaagatgaaa gcgcactggg tttcacactc accactacca
     7321 ataatcgggt ggtgaacact gccgatctat acttcgagcc cgtaggcgaa gtgaccaaag
     7381 acgctaaagg taaccagact tttgtattcc gactgaaaac cgatcagccc ggttcatacc
     7441 tcgatttcat ctacaccatt cctgccaacg attatatggt gaaatatgac atcaaagcca
     7501 cgaacatgaa tgccatcatt ccaccatcca tgaacctgct tgaaatgaac tggagtgcta
     7561 aaatccgtca acaggaaaaa ggccgtcagt tcgaagaaag atattcggca ctccatttca
     7621 agtacctgac cgatgatgtg aacaaactga gcgaatccaa aaacgatcag gaagatccgg
     7681 ccaataaggt gaaatggatt gcattcaaag accagttttt cagcactatc cttattgctg
     7741 acgaatcgtt cgtttccact tcgctcacca gtgaaatgat gcccgaaaaa tcgggatatc
     7801 taaaatcgtt ttcctcaaaa atggctgttg gcttcgatcc agcaggcaat cagcccaccg
     7861 gtttccgttt cttcttcggc cccaaccggt ataaaatcct gtccggctat gataaaggcg
     7921 ttccagggga tgagaaactc tatctccgcg atttgatacc actcggctgg acaatttttg
     7981 gatgggttaa ccgctttatg attatcccca tgttcaacct gttcagtact tttatcggca
     8041 gtttcggtct gattatttta ctgatgaccg ttgtgattaa aatagtgctt ttccccatga
     8101 cctacaagtc gtatgtatca agtgctaaaa tgcgtgtact gaaacccgaa atagatgaaa
     8161 ttaatgcacg aatacccgcc gacaaagcca tggaacgcca aaaagcaact atggaactgt
     8221 acaacaaagt gggagtaagc cccatgagcg gatgtctgcc catgttgctg caaatgccta
     8281 ttctttttgc catgttcagt ttcttcccct cggctatcga acttcgtcag cagagtttcc
     8341 tgtgggcaca tgacctttca gcctatgatt ctatcctgag ctggaatgca tatataccgc
     8401 ttgttacacc gtactttggc aatcatatca gtttgttttg cttgttaatg accatcagca
     8461 gtattatttc caccaaactg accatgtccc agacaccaac tgcatctgac cagcccggag
     8521 caggcatgat gaaatggatg atgtacctca tgccgttgat gtttatgttt atgttcaata
     8581 attatgcttc aggtttgagt tactactatt tcttatccac cctcattacc attggccaga
     8641 catatattat ccgtgctacg gttgacgaca aaaaactgct tgcccagctt catgccaaag
     8701 gcagtaaggc aaaaccaaaa aacaaaagcg gatttatgga acgccttgaa aagatgcaac
     8761 gtgaacagca aaaagcgctt aaaaacagaa agtaaatttc gctgacaaat aaaaataatc
     8821 cattagccgc aaagctttac aaagaatttt ttaactttgt aaaactttgc ggtttttaat
     8881 ttttaaaatc cccgaaaatg aaaaatgtta attatctaat tctacttgtt tttactctgt
     8941 tttttctgtc ttcctgtaca aagaaaaacc ggtttgaaat tgatacaaaa aaagaaaaag
     9001 ttgaagtcac cattcatcgc ttcgaccggg atttgattca tctggacctg aatcatgtca
     9061 gacaatccgt agattcattg taccgtacct atcccgggtt tctgccactt tacatttaca
     9121 atgttcttga tactgttccc acagatacac tggctgttgc cgatatgttt catcagtttg
     9181 tgcaggatac cgccatacag tctgtcaaca aaaaagtgat ggagacattt gctgatgttt
     9241 ctgatatcga aaaagatatt tcaggaggat ttacctacat acatcattat tttccacagg
     9301 ttccgttgcc cgaagtatat ttttttgttt cgggtttcaa ccgttcggtg ctgatgacag
     9361 acagtctgct tggcgtgggc accgattttt atctgggagc cggttatccg ccctataaat
     9421 cattcattta tgagtattta ctccagaata tgcggcgcga aaacctggct atcgatgtgg
     9481 tttcagccac actttttcgc tatttcacat tcgatggtgt caataatgca ttgatagaca
     9541 atatgctgca ccggggtaaa gtgatctggt tgttatccgt tttcatgccc gacaaaaaga
     9601 tggaagacat catgggctat actcccgctc aatggcattg ggccgaaacc aacgaagaac
     9661 aggtttggaa aactattgta gctcagaaac atctgttctc caccgatatg caattgatta
     9721 acaagtacct gaacgatgca ccttttacgg cacctgtgtc gcaggaatca cccggccgat
     9781 tgggaacctg gattggatgg cgcattattg acagctatat ggaacaaaat aagactatga
     9841 cattggaaca attgatgaaa gagaataatt accggaagat actggagcaa tcgcactaca
     9901 gaccctgata acaacaagcc gttcaaaata cactttttat acctgattga atgacatatt
     9961 attcttattt atgagaaaaa tagatacagt cgaagaatta cagttggtat tggaaaaaac
    10021 tgagatcaca caaaaaacat ttcaatcatt ggatttgtct gaatccgaag atctgatttg
    10081 taaaattaaa ttttcaggat gcctttttct gaactgtgtt atgtcatccc ggttaaaaga
    10141 atatttatct gttgataatt acatttttcc tgcgttggat gtaccctttg atgtttaccc
    10201 ttcccgactt tataatagaa agtcacttta cgctgctttt gattatcacc atcccgaaac
    10261 ttactacctc actcacgata aaatagtgta cgactattac cgtaaaatag gcaatgaaac
    10321 ccgaaatata aaggaaacac tggcccgggc attgcacgat cactccataa aagatgaaat
    10381 gaatgaattc ctcgaccagt ttgaggaacg taaaattgtg gctgtaatgg gcggacataa
    10441 gttgggaagg gatgaaaagg tatttattca gttggctcaa ttgtcaaaac aacttactga
    10501 aaaaggattt ctgatgatta cgggcggcgg tcctggtgcc atggaagcta cacacctcgg
    10561 tgcctggttt gcaggtaaga caaatgatga acttaacgat gctgtgagaa tattaagtgt
    10621 ggcaccatta tacgatcatc ctgaatggct ttctactgct attcatgtca tggaaagata
    10681 tcccgaaacg gggtataaaa gtctgggaat acccacgtgg ctatacggac acgaactttc
    10741 cacacctttt gcaacgcata ttgccaaatt ttttgaaaat agcctgcgtg aggaaggtct
    10801 gttggcagtg gccaaagggg gtgttatttt tgctcccgga agtgccggta ccatgcagga
    10861 aatttttcag gatttggcac aaaatcatta tgaatcctac ggatatgcca gtccgatgat
    10921 atttctcaat cagacatact ggactgatga ccgaccgata tatccactta ttcagaagat
    10981 gattgaaaac agagatttga aaaatattga tttgtcgctc tacgatgaaa actccgatat
    11041 cattgctcat ctcgagaaat ttgtagcaga aaatcaatga cattatgtgg ctttctatga
    11101 cgaatatggt aacccaaagc ttttatttat attattatgg aagaaaacta cttatctatt
    11161 aaaaattggg ctgaggatga ccgtccacgc gaaaaactcc tgaataaagg tgcctcggtg
    11221 ctttcagatg ccgaactgtt ggcaatcatg atcggctcgg ggagcagaaa tgaaagtgct
    11281 gtgcaacttt gccgcaaaat actgaaagcc tcacacgaca acctgaacga actggcacga
    11341 ttgtcaatag atgaattgtg caagttcaaa ggtattggcg aagcaaaagc aatcactatt
    11401 agtgcggctc tggaactggg caatcgcaga aaaaccaacg aagtgagaga acgcgcaaga
    11461 attactcaaa gccgcgattt gttcgaactc tttgagccca tactgctgga tttgaaacac
    11521 gaggaatttt gggtggcact gctcaatggt gccaacaaag tgctggatgt gaagcggctc
    11581 acgcagggag gaacccgaca gacggtggtg gatataccca tgatcctgaa aatgtcactc
    11641 gaaaaatcag ctccggccat tgctgttgca cacaatcatc cctcgggaca ggataatccc
    11701 agccgtgagg atgatgccat tactcagcga ataaagaaag gatgtgaagc catagatata
    11761 aggctgttgg atcacattat cattgccggt gggaagtatt acagctttgc cgacgaagga
    11821 aaaatataaa caaacattac aaatgaagaa taccaaatta aaagatatta attataaatt
    11881 accaaaaagt cccccttcag ggggatttgg ggggcttata tatgaattta ttagaaataa
    11941 ataacgttgt aaaacagtat tccggtcagc tggctctcaa cgatgtcagt ttgattgtgc
    12001 cccgtgggtc ggtgtatggt ttgctgggac cgaatggtgc cggaaaaacc acattgattc
    12061 gcatcatcaa ttgcatcacc gcacccgaca gcggcgaaat tctccttaac ggcaaaaaat
    12121 taactcctgc tgatgtgcga aatatcggtt atctgcccga agagcgcgga ctgtacaaaa
    12181 agatgaaagt aggggagcag gctatgtatc tggcacggct caagggaatg acaaaacacg
    12241 atgctacaaa agcgcttaaa tactggttcg agaagtttga aatacaggat tggtggaaca
    12301 aaaaagtgga agaactctcc aaaggaatgg cacagaaagt gcagtttatc actaccattc
    12361 ttcataatcc ggatctgctg attttcgatg aaccgttcag cggatttgat cctgtaaacg
    12421 ctgatattct gaaaaaagaa attctggccc tgcgcgataa gggggcaacg atcatttttt
    12481 caactcacaa tatggaatcg gtcgaggaat tgtgcgagaa tatctcactg gtcaacaaag
    12541 cgcgtattgt tttgcaggga aaagtggctg atatccgggc ttcgcaatcc accagaagcc
    12601 ttgtaatcag atcatcaacc ccatttacgc cctacaattg caatattatt tcccaaaccg
    12661 acaagaatgg gatgttcgaa acaacgcttg taaaagggag caacctgtca aataatgaac
    12721 tgcttgcaga gctgatgaaa caaacggata ttgtttcctt cgaagaattg ctccccacca
    12781 tgaacgatat tttcatccga acggtgacca cacagccccc taaaggtgga gtataggtac
    12841 gtttatcaat attttctaat ataaaaaaat gtctttctaa attcaactaa cttaattaaa
    12901 gctcttaatt cccccttcag ggggttaggg ggcaacaaac tatgagtaag ataggaataa
    12961 ttattagcag ggaatatact acccgcgtca tgaaaaagtc gtttttactg ctgacttttc
    13021 ttacaccatt gctgtttgtg gccatgatca tgataccact ctggttgtcc aatatccagg
    13081 acgatacctt gaaaaacatc attgtcatag ataaaaccgg cttgtatcac gatgcgctga
    13141 agagtaacga cacttatacc tttgtgtttt cggacaaacc gctcgataat ttccggaaaa
    13201 aagaaaaaac cgggaatgaa ctgactgctg tgttgtatat caccgccgat ttgagcacca
    13261 aacccaatgc cgtatcactt tattctgaaa aacaggtaaa tgtcgagttg aaatcttaca
    13321 tttccggatt gttgggagat tttgtgcaga aacaaaaact ggccggatat aacattccca
    13381 acctgaagga agtggtggaa aaatcgaaaa caaatgtcga tatttcgact ataaaatgga
    13441 atgctgatgg tcaggaaaag gaaggttctg cggagttggc attgattatc ggtatgattt
    13501 ctgcttttct gatttatatt ttcatcatgt cctatggctc tcaggtgatg agtggcgtat
    13561 tgcaggaaaa aacaagccgg attgtggaag tgataatttc atcggttaaa ccgtttgaat
    13621 tgatgatggg taaaattatc ggtatcgcat tggtcggact tacacaattc tttatgtgga
    13681 tagctcttac acttattatt acagccgggt taagtgtgtt tatgggagga ggcattgata
    13741 cggaagctat acaatccgct caacagatca atatgcctgg tatgcaatcc ggcatgtcgc
    13801 aatcggatac tcaatccatg attactgaaa tctataaaag tctgaatgga ttcgacttct
    13861 tcaaaaccct ggtgttgttt atcatttatt tccttggcgg atatctgctg tatgcctctc
    13921 tttttgcagc tatcggctct gcggtcgata atgagacgga tacacagcaa ttttccatgc
    13981 cattgactat acccatttta tttgccattt atgcagccat ttatagtgct caaaatcccg
    14041 atggtccgct tgctttctgg tgttcgctca taccattcac ttcgcccatc gtgatgatgg
    14101 tgcgactgcc atttgatgtt cccgcctggc aaatcgtatt atctatcgtc atattggtcc
    14161 tttcatttat cggtaccacc tggatagccg gcaaaatata ccgcaccgga attctcatgt
    14221 acggcaagaa agtaagctgg aaagaactct ggaaatgggt gagatacaaa taaataaaaa
    14281 aaaatcagag cttttacaac tctgattttt ttttatgatt actgcttcat tttaggtctt
    14341 gtatcttcat tataccattt tattttatgc gacagataca tgatgatgcc cagtattaca
    14401 aataatccga tgcttcctat cagcaatgct acatcttcca gttgtagaac tacgtacagg
    14461 aaaacgtata agataccaag cagaaatgcc aggataccgg tttgtgtttt gtttttgaag
    14521 atactgtttg cataaaccgt tatcagaccg atggtcgcaa tactggctat caggtaggag
    14581 attccaaaat ttaattgttc cgaaatagag agaagcaaac tgtaaaacag gattaatgct
    14641 acaccaacca gcagatattg tattgggtga attcttttac gggtaagcac ttccacaaaa
    14701 aagaacacta cgaaagtcag tgcgataaac atcagtgcgt atttgtttga gcgcatgttc
    14761 agttgataat gatccactgt atcaaccaga tttacaccga atgaattatc ggcagtatca
    14821 ataatattac catctttcca cgattccggt atgtttctgt tgaaatgaag tatattccac
    14881 ctggctgtaa aaccggtttc gttgatctta tattgaggtg tgaaatttcc aataaatccc
    14941 ggagctttcc agttgccttc aacaaatacc tgcgtactgc gtcccactgg tataaaatta
    15001 aggttgctgc tgccattcaa atccatattg caatgaaaat caagtctttt ttcgggttga
    15061 aataagacag catttttcaa cgagattatt agtccctgac cgatggcatc atcgctgcct
    15121 ccggtttctg cagtaaaatg ttgtttatta atttcaaagt caatattttt tgtaatgcct
    15181 ctgaggtctg ataatcccag acggatatat gcatctcccc attgtggttc accttctatt
    15241 tgcttttggt ctatagcttt aaaattaccc gaaatatcca gtgaactttt atacagtatt
    15301 gatttgtata tgccataatg acgttcttca ggagttagtt tcgatttaat tatcaacgat
    15361 tcgggagcca gattgaatgt atggttctct gtttttgagt tttttttctc gtcggtggtg
    15421 gtgattttgt atggaataac caaaacaggt ccggtgattg tttgagcatt gctccatttg
    15481 gaattgattt tttcaattgt ttcccgactt ctgttttgtc gctcgtgaat taattcctgt
    15541 atcatactac ccggtatcaa cattagcaaa gttaatacac cgataatgat tgctttcagt
    15601 gtgactgact gactgaattt ttcaattttt tcgtttgttg tttccataaa aatgaatgaa
    15661 ttaatgaatt tataaaaaaa gtactttgaa atgcaaagtt aatgtataaa aaaataaatc
    15721 atccaatcag gttggacaac gcatctaaat ggtcttgaaa actggttcgt ccctgatcgg
    15781 tgattttata tctggtattt ggtttgcgcg aaatgaactg cttgattgtc tgaatatatc
    15841 ccaggttttc gagcgctttt atatggcttg caagattacc atcggtcaat tccaataatt
    15901 ctttcagcgt attgaaatca actgtgtcat taacaatcag aaccgacatt attccgagtc
    15961 gtattctgct gtcaaaagct ttattcagat ttgctatgat attggccata gttagttcag
    16021 gtcatatttt ttgtacataa taaatccata aaccacgtgg caaatgccaa accctattgt
    16081 ccataatatc agaccactgt gaatgaaaac ggctgctaat aatccaatta taatttcagc
    16141 aattccaagg taatgaactt caccgtaagt gaattttgaa acatttacaa gagccagtcc
    16201 atagaatata agcgtagtgg ctgcaacaag ttctgtttgt ccccggctaa tcattattat
    16261 actcagaata ccacctgcag ccagcggaat gcccagatta tacatcgtat ttagtacaac
    16321 cgtacttaaa agcttctgat ttttcttctt tgcttttctc cgtgaaaaat aaatggcaaa
    16381 tcccagcgaa ataactaata ctaaaatagc atcaactgta agtattaata aatcctgttt
    16441 cagtagaaat tcggcagaag aatgatgacg taacaggtac aaataggcaa acaaagcacc
    16501 tgcaatggct gtcactccgg ccatgatacc cgacaatcca ctcagtgaca aaaatttgga
    16561 tgatttttcc atcatctcac gtattgcctt caaatcttcg gtatgttttt ccattatgtt
    16621 ttcaatatta aaagtacttt gaggcgcaaa gctaaacaaa taaatcagat atattaccgg
    16681 atttatttta tttaacaata aatttatttc tatggagttt aaaacaaaaa aatcagaacc
    16741 tgtagaggtc ctgattttct gaatgttttg tagagaatgt gatgaaaagg ttagaattta
    16801 tgacgacctt catcagcaac agttagtttg gctctaccct tagcacgacg agcggctaat
    16861 actctgcgtc cgtttgcagt tgccattctt tcgcggaaac catgtttgtt tcttcttttt
    16921 ctttgtgaag gttgaaaagt ccttttcatt gctatttgtg ttttattttg ttatattttt
    16981 tgtctgaatt tcggactgca aaaatagtga atttctttga atatgcaaaa taatatttga
    17041 aataatatta ttttcattca caattcctta ttacttattt ctgctatagt tttcacactc
    17101 ttaggtgttc attccaccgc atacattgat tacctgtccg cttacatagc tggagaggtc
    17161 tgaagcaagg aaaacacata ctttggctac ctcgtcaggt gaaccgccgc ggcgtaatgg
    17221 aatagtttca gcccacttag cccgctgttc ttcagtcagg gcgtgagtca tttcggtttc
    17281 gataaatccc ggagcaatag cattggcacg tatgcctctt gtgccaagtt ctttggctat
    17341 tgattttgcc agacctatca taccggcttt ggaagcggag tagttggttt gaccggcatt
    17401 acccgaaaca cccactacag aactcatatt aatgatactt cccgattttt gtttcatcat
    17461 tacaggtgtg caggcatgta caaagttgaa tgccgatttc agattcacat ttattactgc
    17521 atcccattgc tgttcggtca tgcgcatcat caggccatct ttggtgattc cggcgttgtt
    17581 taccagtata tccacccggc caaagtcttt aacgatctca tccaccacct gatgtgtggc
    17641 ttcaaaagaa gctgcatttg aagcatatcc tttggctttt acgccaagtg cttccagttc
    17701 acgttgagtt gccagaccat tttcgtcgat taccaaatca gtaaacgcaa catggcatcc
    17761 ttcacttgca aatttgagag ctatggcctt accaataccg cgtgcagctc cggttacaat
    17821 agctactttc ccttctaata atttcatttt attatttttt tggtgttaat tatcggctgc
    17881 aaagatacgg attttttgat aaaattccga tttcaggctt taatggaaaa tgaaaaaggg
    17941 aataagccga agtctattcc ctcctgaaag gttgaaattc aattatttag ctgaatttat
    18001 tcggcttccc cgatggattc ttatattgta ttgatcagtt tccgtagcaa cagcccggtt
    18061 ggttttaact ttctgaatgc gagatttaat gccttgtgct gtgctggctt tatttactaa
    18121 tcctaaatca ccgttataag tgcccgaaat agtgtttcca agttcgtcta tgaaggtaaa
    18181 atttagattg taacttgatc cggcattttt aacaactgtc atggaaccac ctgtgatggg
    18241 gaaagcatcg gctgtatagt accaggttcc caggtcttcg gttccgttat taaatccgtc
    18301 tattactgtt tggttttcaa cggtgtttat ctttaccgga tacgtgccgt tgggcaggga
    18361 agtggtgtac gatgtgggtg tgtacacttc tattcgcatc agatcgccgt tgccgctcat
    18421 attggtcata ttgatattct gatcggccag ccaaatggtg tatatgttta atcctacctg
    18481 atcaatgtcg ccccagtacc agagttgtcc ttttttcagg gtttgagggt aaggtgtcac
    18541 caggtcggtg gtggggatgc tgcccgaaaa tgagaattcg taaaactcat tgtcactggt
    18601 cgtaacctgt ccggtgatgc tgtaatttcc cattttattc acaataatgc tgccgcttga
    18661 aaggaagaga taatcggtga tttttccgtt attcacaata gcaatatagc taccgctggg
    18721 cacatcttca ttattgttgt tcagtaccat ttttccacgc tggaaggtta attctacact
    18781 ttcattcaca ttttcgttat ttactttata ggttccggaa gggatggagg tggcattttt
    18841 agtggtattc atatccagaa acaaaaaagc tccgcttccg gtgatgtatc cgttggcatc
    18901 ggttcccaag ccgctgtcaa agaaataagc ctcaaaagca tttacattgt ttcgatatat
    18961 attgccaaga taattggctt cagccttgat aaagttgaaa ttgcttttgt ttacatcggg
    19021 attttttttg caggctgaaa atagcatagc aacactcatt aaaagcattg ttattttgaa
    19081 tgattgtttc atggcttgag ttttttttaa tgggtttaaa tttctatagg aataataaat
    19141 caaaaactgt gcctgctttt tgcagaaata atatttttca ctattaaaaa caaaaaaccc
    19201 cgaaaaatcg gggttataat gtgatttatt ttaggctgag cggattattt tttttctccg
    19261 cgattgccat aacggttcat aaacttatca acacgtccgg ctgtatccac cagtttggat
    19321 tttccggtgt aaaaagggtg tgatgaactt gaaatttcta actttaccaa tggataagtt
    19381 gcaccgtcaa tgtcaacagt ttcctttgtg ttaactgtag aacgagtaat aaaggtatcg
    19441 ccgttggaca tgtctctgaa tgctacgggg cgataatttt ctggatgaat tccgcttttc
    19501 atatcttaat ttttttattt tattgcgttt tatttgggct tgcaaaatta caaataataa
    19561 tcaaagtatc aaattatttc ggtaaatatt tattttaatt tcgttgaaaa attatttttc
    19621 agcttattaa gcgttttaac acggttgttt gtggcgtcaa tgcattggtt taatctcccg
    19681 tatagatatt ttcgggataa gccacctcca ccagaaacaa ccctttggcg ggcaccgaac
    19741 ttcctgcgtt gcaccggttt ttggcatcta ttacctgtct gaattcatct atcgtcattt
    19801 ttccccggcc tacttccagc aatgttccca ctatggcacg caccatattt ctcaaaaacc
    19861 ggtcagcttc gacgatgaaa atccattctt cctccctttg ctcccagcgg gcagccatta
    19921 ttttgcagtt attcgttttt acatcggtgt gcagtttgct gaaactggta aaatcggtgt
    19981 attcaaacag aatttttgca gcttcattca ttctgtcgaa atccagcgga ttataaattc
    20041 gcatagcaaa gggatgacga aaaacatctt tttgtagtac caggtgatat tcgtaccggc
    20101 gcgagagtgc atcaaagcgc gcgtggactt cgggcttcac ttccctgatt tctatgaccg
    20161 aaatatctga gggcaataaa ctgttcagtt tattgacaag atccaggcct gccggtaacc
    20221 ggtcttcaac atcaaaatga gcaaccatgt tgcgggcatg tacacctgca tcggtgcgtc
    20281 cggctgccac tatttcaata tcttcggttc gtagtatggt gcacagggct tttgtcagca
    20341 cttcctgcac cgacagtccg ttgggttgaa cctgccatcc gcagtaggca gtcccatcgt
    20401 aagcaaatct gatgaaatat cttcttttca cactgcaaag atacaggttt tgaaagcaat
    20461 aaaaaagcat tgactttacc ggtcaacgct tttctgtaat ttgttttttc agatttagaa
    20521 attgatctgc aatgtcatcc caacgggtcg gtcggttata cccggatatt tcttggtttc
    20581 catataaggc ttcaactgga tgcttacctg atcttttctc aaatcctgca tatacatatt
    20641 gttgactttg gccactttta cggcatcaac tatggaccat atgtaggtac ccagcatagc
    20701 tacaccccct acaataaata atccgaaacc caactcggca ttatttctgt tgtaactgtg
    20761 attaaattcg ctgtcataat cgtagctgta atctccctga gcaattgcca ttccggtcat
    20821 acagataaat gaagtgccca tgtatccgcc cagaaatcca agtccgcgtc ccacttcgcc
    20881 gctgagcatt tgacccaggc cggggataaa gaacgaggct ataccggcta ctgaaggatt
    20941 atacgggtcg cccggttgcg gaatgtacag ggcggagttg tatcgcaatt tattctgatt
    21001 gaatttttgc cccattctca ccggaggtcc actttgttcg gctacccggg tgtcgttaat
    21061 aatccgcttc tctcttattt ctatctgttt gcgcaccggc acatcgttga tatactcaat
    21121 ttcctgttct accggcaatt cttctgtcac atcgtattgc tgcacaaagc cgtttttaaa
    21181 ccgaatttca tgaaccttaa atttctcaag gatgtaatcg ggtccgtcag gattctcctg
    21241 aagtttgtat tttattccac cgggaccaat ctcagaaatt ttggcaataa tctgactgct
    21301 gtttttcagc acaattgtat cctgagcttt cagacttgat gttaggcata tcaataccga
    21361 aaaaaggaag aaccaacgat ttttcattct gataaattta tgtgttaaaa atttatgtaa
    21421 ttgatttaat atgaccgata tgcgaatctc aataatgccc gtatttaaaa cgcaccggta
    21481 aattccccca gaaaacgcac atcattttcg gagaacaatc gaaggtcttt cacctgatat
    21541 ttcaggttgg ttatgcgttc cactcccatc ccaaatgcat aaccagagta aacttttgaa
    21601 tcaatgccgc agttttcgag tacattgggg tctaccatac cacagcccag aatttctacc
    21661 cagccggtgt gtttgcaaaa tgcgcagcct tttcctccgc agagattaca agaaatatcc
    21721 atttcggcac tcggctcggt gaacgggaag taggagggac gcaaacgaat ctgcgtatca
    21781 gcgccaaaca tttctttggc aaaatagagt aatgcctgtt tcagatcggc aaacgataca
    21841 tttttatcga tataaagtcc ttccacctga tgaaaaaaac aatgagcacg atacgaaatg
    21901 gcttcgttgc gatacacccg ccccggaaaa atcatccgga tgggtggctt ttgagaggac
    21961 attacgtgtg tctgaaccga agatgtatgt gtgcggagca gaatatcagg atttttctca
    22021 ataaaaaatg tatcctgcat gtcacgtgcc ggatgttcgg gcggaaaatt aagtgccccg
    22081 aatacattcc agtcattttc tatttcggga ccttcggcaa tggtaaatcc gatgcgcgaa
    22141 aatatatcgg caatttcctg ttttacaagt gataacgggt gacgggtgcc caatttgacg
    22201 ggctcggcag ttcgggttaa gtcggttttt tgctctgata cacccgaggt gctgagcgat
    22261 tctttaagtt cgttgagttt atcctgtgct ttgtttttta attcgttcag tagctgacca
    22321 acttcccgtt tatcttcatt ggctacgtta cgaaaatcgt tgaaaagact tgtaatttca
    22381 ccctttttgc tcaaatattt gatacgtaat gcttccgctt catccagtgt gctcacccga
    22441 agtgaattta tctcatccat taattgctga atcttattct tcatctgttt attattttat
    22501 tgaaaatgtc gtaagttagt aatctgattg atattgtgtt ggttaaaaat agtaaccttt
    22561 ccaatttgtt tacaaaagta atcattttcc ctgaaatccg aagttgaaaa gaaaaaacaa
    22621 tacctgaaac atgaataatt ctaaattttt tacctatttt tgtaacagat aaatatacaa
    22681 gagacgggat gaaaaatata cttgatttcc tttcggaact aaaaaatcac aacgaccggg
    22741 tttggttcag cgagcataaa agtgaatatg atacagctcg aatcgacttt gagcaaatct
    22801 gcagttctct gattctcaaa atagcagcct tcgatgaaga tatccgcctc gtcgacgtca
    22861 gggactgtgt attccgcatc taccgcgata tccgtttttc accggataaa acaccttata
    22921 aaacacattt tggagcattt attgcttcag ccggtggtcg taagagcgac cgcggaggtt
    22981 attatcttca tttggaccct gccggatgct ttttctcaat aggagtatgg agccccactc
    23041 cggttgttct taaggcgatt cgtcggagta tctatgataa tattgatgaa ttcaatgaaa
    23101 taagaaataa tcccgatttc cgcactttat ttaacgattc cttttacgaa gaagataaac
    23161 tgaaaactgt tcctcaggga ttttcaaaag attttcccga tgctgaattg ctgaagttga
    23221 agcattttat ggtgacgtat gatatagatg acaatatgct taattcttct gaatttgtgg
    23281 aaaaactggc tgaaatagcc gaaaaatcat atccgttcct tcaatttctg aattattcgg
    23341 tggacgaggt aatttaattc tgtaacgtaa tatttaaaac gacaaaatat gaaaagttca
    23401 aaaatgctgc ccatagttgc atctgatccc tggctcgaac cctatgctgc tgccatacag
    23461 ggaagatatg actattatct ttcggttgaa aaacggttga cgggcgaaaa aggcagtctt
    23521 tccgatttcg caacgggtta tttgtatttt ggacttcatc atactcccga tggctggatt
    23581 ttcagagagt gggcacccaa tgccacggct atttttctga ttggtgattt caacaactgg
    23641 cagaaaaaca aagaatatca actcactaag cttgaaaatg gtgtgtggga aattcaattg
    23701 cccgaagatg caatgaagca cctgcaactt tacaaactga tagtggaatg ggaaggcggc
    23761 tcgggtgagc gcatcccggc atgggcacgc agagttattc aggacgaaca taccaaaata
    23821 ttcagtgctc aggtttggaa tcctgaaaat cccttccagt tcaaggtgga taaatttgtg
    23881 cccgatacaa aacccctgct catttacgaa tgccacatcg gaatgtccag ttccgaagaa
    23941 aaagtatcta cttacgatga gttccggcgt accgttttgc cacgcatagt gaacgatgga
    24001 tataactgca tccaaatcat ggctattcag gagcatccct actacggatc tttcggatat
    24061 catgtttcca gtttctttgc cgcgtcgtcg cggtttggta cacccgaaga gctcaaacgt
    24121 ctgatagatc aggcgcacga aatgggagta gcagtcatca tggatattgt acattctcac
    24181 gcagtgaaaa acgaggtgga aggtctggga cgatttgacg gaactcccta tcagtatttc
    24241 cacggaggcg aacgccgcga gcatcccgcc tgggattcgc tgtgcttcga ttatgccaaa
    24301 ccatccgtgt tgcatttcct gctttccaat tgtaaattct ggctcgaaga atataaattt
    24361 gacggtttcc gattcgatgg cgtcacgtcc atgctttacc gcagtcacgg gctgggcgaa
    24421 gatttcaacg gatatggttc ctattacaac ctcaatcagg atggcgacgc tatttgttat
    24481 cttacactgg tcaataaact tattcatgaa gtgaatccac atgccataac catagccgaa
    24541 gaagtgagcg gaatacccgg actggcttcc aaaatcagcg atgggggatt tggtttcgat
    24601 taccgcatgg ccatgggaat acccgatttt tggatcaagt atatcaagga agtgaaagat
    24661 gaagactgga aagtaggcca tatttactgg gaactcacca accggcgggc cgacgaacga
    24721 accatcagtt atgccgaaag tcacgatcag gctctggtag gcgacaaaac cattattttc
    24781 cgcctcattg atgccgatat gtactggcac atgcaaaagg gtgatcaaaa tttcagggta
    24841 gaacggggta tcgcactgca taaaatgatt cggctggtga cttctaccac catcaatggc
    24901 ggttatctca actttatggg caatgaattc ggtcatcccg aatggattga ttttccccgc
    24961 gaaggcaacg gctggtcgta caaatacgcc cgccgtcaat gggaattagt ggataatccc
    25021 gattatatct atcacggact gggcgaattt gaccgggcaa tgatacggtt gattcattcg
    25081 gtgaaacatt ttaatgaaac gcctctttac cagctttggg acaatgaagg cgatcaggtg
    25141 ctggcttatc aacgcaacga tttggtgttc gttttcaatt tccacggaac aaattcatac
    25201 accgattatg gcattctggc cgaaccggga agctataaag tagtgctcaa taccgataat
    25261 ccgctcttcg acgggcatgg cctgattgac gaatccatag aacatttcac ccagcccgct
    25321 cccgaagaat acatgggcaa gcaatggctg aaactctaca tccccgctcg ctcggctttt
    25381 gtgctgaaga aacagtagta ttctggaaaa taaaaaataa gcccgatgta gcgtgaatgc
    25441 tgcgtcaggc ttttttttat gagaatattt tataactgca tgttttgtca actcaaattt
    25501 ttaattttgc caaaattatt gattaaaaag aattatggac gaaatacaac ttaacgaacc
    25561 aaaaaaagac tacaacgagc ccatgcatac tgctgagcat attctgaacc aaactatggt
    25621 caggatgttc aattgtggta gatccaaaaa cgctcatatc gagcggaaga aatcgaaatg
    25681 tgattatctt ttatcgcaag ctccatctgc tgagcagatt aacgaaattg aacacagagt
    25741 aaatgaagta attcaacaaa atctgcctgt aacagaagaa tttcttacat acaaggaggc
    25801 tgctgctttc gtggatctct caaaacttcc cgatgatgtg tcggaaacat tgagggttat
    25861 caaagtcggc gattacgatg catgtgcctg catcggtcag catgtgaaaa acacctcgga
    25921 aatcggacat tttgaaattc tgacacacga ttttgaaaac ggacgctggc gcgtacggtg
    25981 gcgggtgact gcaattaact aatcattatg gcaatgaaga agttagctaa actcctgcga
    26041 aatttagtca ttttattttt tgcttccagt atcttagcgg tgcttgtgct tcgttttgtg
    26101 ccggtatata ttactccttt gatgcttatc cggtctgtag aagcaatggt tgacggtgag
    26161 acgccgaaaa ttaagaaaga ctgggtacca ctcaataaaa tctcccggaa tatggttcag
    26221 gcagtggtag catcggaaga taaccttttt atggaacaca atggtttctc tttcagggat
    26281 atagaaaaag cattggaaca taataaaaag ggaaaacgta tccggggtgg aagtaccatc
    26341 tcgcaacaga cagcaaaaaa cgtattccta tggccacagc gttcgtgggt gcgcaaagga
    26401 ttagaaacct attttaccgt cctgattgaa ttcctgtgga ccaaagaacg gataatggag
    26461 gtttatctca atgtaataga aaccggcaat ggaatatatg gtgtacaggc agcatccagg
    26521 aagtatttcg gggttaatgc ctcaaatctg acccgttcgc aggcagccct gattgccgtt
    26581 tgcctgccca atccccgtaa atttagccct gcaaatcctt cgggttatat ttcccgtaga
    26641 aaaaaccaaa tagtgaattt aatgggaaaa ctgaaaagag ttgattttga atgaaatttt
    26701 taatagttct gaaaatgtat aacagcttga aacagccatt agagataaat tttaaacaca
    26761 aggaaatgat taatggcgtg ttccctctgt ccttaattaa aaaataataa aaaataaaca
    26821 gatgaaacgc ccatgtccaa cctaaaaaaa tggacacaca atggcatgat taaataacgc
    26881 ataacaaatg atagacgctc aatattcctc tgtcagctga cggatttggg ggccgataaa
    26941 taaaaaataa acagatgaaa tacgatgatt ggggattaat tgaatataaa caggcctggg
    27001 aaaaacagga aaaattgttc acagaaacac tgaaccgaaa agcagaagga cttcagaccg
    27061 aaaatcacct gattttttgc gaacatcccc acgtttatac ccttggaaga agcggcgatg
    27121 aaaacaatat gctgctggat tacattcaat tgcaggcaaa gaatgcttcg tttgttcata
    27181 ccaatcgtgg tggcgacatc acgt
//
LOCUS       DAOPZK010000010        26482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1815_length_26482_cov_22.594430, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000010 DAOPZK010000000
VERSION     DAOPZK010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26482
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1815_length_26482_cov_22.594430"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKH68_01170"
     CDS             <1..656
                     /locus_tag="PKH68_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNX11574.1"
                     /translation="APVTNFALAGMSGLMSGVMHAPLTGIFLIAELTGSYNLFMTLMI
                     VSVVSFLTIIIFEPHSLYAMRLAQKGELITHNKDRAVLTLLKMENVIETDLTELTPTM
                     TLGQLVTVISRSKRNIFPVVEPQNRILMGVVLLDEVRNIMFHPEHYNRFTVAQLMISP
                     PAIINVNMTMEKVMQIFEDSKAWNLPVVDDQKKYLGFVSKSKIFNSYRHVLVHFSEE"
     gene            669..869
                     /locus_tag="PKH68_01175"
     CDS             669..869
                     /locus_tag="PKH68_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNX11575.1"
                     /translation="MGKKIMYSDAVAELTKILADLESSGSEINMELIEEKVKRAAYLL
                     DLCKKQLHEINTELEVILENLE"
     gene            complement(872..1672)
                     /gene="pgeF"
                     /locus_tag="PKH68_01180"
     CDS             complement(872..1672)
                     /gene="pgeF"
                     /locus_tag="PKH68_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNX11576.1"
                     /translation="MSSNTNPCLIQYQILKRFDSIRHFTTTRIGGVSVGNYSSFNLGL
                     FTSDLPENISQNFDLLGSLTGIDKNTIHLPFQTHSNRIFQIGENFPGQHDETKKSLLH
                     GIDALITDIPGQCIGVSTADCVPILLYDPVCKAIGAVHAGWRGTCSGIVTKTITAMKS
                     AFETDPANLVAVLGPSISPDAYEVGAPLMDEFREAGFETKEIFSEKNGRIFLDLWKAN
                     KQMLTDGGVKEENIEISGICTYSQHENFFSARRLGISSGRMISGIMLI"
     gene            complement(1662..2801)
                     /gene="obgE"
                     /locus_tag="PKH68_01185"
     CDS             complement(1662..2801)
                     /gene="obgE"
                     /locus_tag="PKH68_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNX11577.1"
                     /translation="MAGSNFIDYVKIFCRSGKGGAGSLHFHREKFITKGGPDGGDGGR
                     GGHIILRGNSNLWTLLHLKYSRHIYAGDGGDGSKSRSFGKDGKDQLIEVPCGTVVYDG
                     ETGEFMCDITNHGEEVILMKGGRGGLGNWHFRTATNQAPRYAQPGEPRVEKTVVLQLK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLTPNLGIVAYRDNKSFVMADIP
                     GIIEGASEGKGLGLRFLRHIERNSILLFMVPADASDIKNEYNILLNELQKYNPELIDK
                     QRILAISKCDMLDEELIEQMKKDLPDDVKTLFISSVSGAGISELKDMIWEELNKETNK
                     LVEMVHKPIEVAEMLTEEDEMEIEVDEFDDEDDLSKYKGIGWDEQ"
     gene            complement(2935..3513)
                     /locus_tag="PKH68_01190"
     CDS             complement(2935..3513)
                     /locus_tag="PKH68_01190"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNX11578.1"
                     /translation="MINIIICGAPGCGKGTQSDLIVKKYNLTHISTGDLLRKEISDKT
                     ELGTVAHQYISQGQLVPDEMIIEMLARKIDTLNHSQTNGIILDGFPRTLPQAEALEIL
                     FQKRGTQTDILLDLQVDEGELITRLLLRGETSGRSDDNMETIKKRLDVYYLQTKPVSE
                     YYKSLGKYVEIDGIGTIDEIFGRISAAIDEKL"
     gene            complement(3555..4091)
                     /gene="hpt"
                     /locus_tag="PKH68_01195"
     CDS             complement(3555..4091)
                     /gene="hpt"
                     /locus_tag="PKH68_01195"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNX11579.1"
                     /translation="MKTIQIKDKQFTLSIPSEDILKAVQTVGDAINRDLKDKNPLFIC
                     VLNGAFMFAGDLMKVVDIPCEITFIKLSSYEGLFTTGTVKEVMGLNESVVGRNVVVVE
                     DIVDTGITMDKILTSLQAKGANEIYVATFLQKPAALRRDVRVDYVAMKIPNEFIVGYG
                     LDYDGYGRNLKDIYTVID"
     gene            4304..5440
                     /gene="dnaN"
                     /locus_tag="PKH68_01200"
     CDS             4304..5440
                     /gene="dnaN"
                     /locus_tag="PKH68_01200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNX11580.1"
                     /translation="MKFVVSSTELLSHLQAISRVINSKNSLQILDNFLLSLEGNTLTM
                     TASDIETTLITSMEVQDVEGSGKVAVANKLLLDTLREFSEQPLSFYIDDSNLAMVITS
                     SNGTYNFIGQSGDMYPQLPLLEDNARQLNISVPVLFSGISKTFFCMADDELRPVMNGI
                     FFDIAPDSLTLVATDAHKLVRFKSAYLSASIKEDETFNFILPKKPAIMLRNILPKESG
                     EVNIHFDAKNAYFKLSNYTMVCRQVEGRYPNYNGVIPKNNPYKVIADRVTLLNALKRV
                     SVFSNQASNLIKLVFNQNQIHVSAQDIDFSISAEEYIPCQFEGEEIKIGFKSTFLIEM
                     LGNVDASDIVIELADPSRAGIILPFENAENEDILMLLMPMLLND"
     gene            5537..6304
                     /locus_tag="PKH68_01205"
     CDS             5537..6304
                     /locus_tag="PKH68_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNX11581.1"
                     /translation="MKLNLKNPIVFFDLETTGTNIVSDRIVEISYLKISPNGREESKT
                     IRINPEMHIPESSSAIHGIYDQDVADCPTFREVAKEIIHDIEGCDLAGYNSNRFDIPL
                     LAEELLRADVDIDLMKRKFVDVQVVFHKMEQRTLSAAYKFYCEKDLENAHSAEADTMA
                     TYEVLQAQLDKYPELKNDIEFLSKFTAQTNNVDFAGRIIYNEKGEEIINFGKYKGQKV
                     TDVLKTDIGYYGWIMNSDFTLHTKKVLTNIKLRDFNK"
     gene            6423..7055
                     /locus_tag="PKH68_01210"
     CDS             6423..7055
                     /locus_tag="PKH68_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11582.1"
                     /translation="MRRLKFILFVLLAAICFPVVITAQTVSVTDRTPEQEAIKQTEKL
                     QSELKLTDEQAKIVYEINLRYARERQLSNSRSQAMERIKNKNEEIQRVLSPQQYDELL
                     YRKNDVQTVEIDNAKRYIHTNPQNRVNNTDVKSPALRTRNSDPGVEDQRSLRMGIRQN
                     ESVAQPRTGRSVETNSRSVVPTRSSETVTTRSSSGRTSESGSSTRPATRR"
     gene            7074..7787
                     /locus_tag="PKH68_01215"
     CDS             7074..7787
                     /locus_tag="PKH68_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNX11583.1"
                     /translation="MSNTWFKFKQFTVSHDKCAMKVGVDGVTLGVWADVAEAKKVLDV
                     GCGSGLISLMIAQRCDAQITAIDIDNQSIIQTAENVDNSPWKDRIKTQPISLQEYARV
                     TSDRFDVIISNPPFFNNSLKNPSESRTTARHTDSLSHEDLLMCSRKLLHPDGKLCLIL
                     PVEEGNACIETGKETGLFCSKKVSIFPNSSKPAKRLLLEFILSETACVERNLTIETDV
                     RNQYTPEYTNLVKDFYLKL"
     gene            complement(7800..8057)
                     /locus_tag="PKH68_01220"
     CDS             complement(7800..8057)
                     /locus_tag="PKH68_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HNX11584.1"
                     /translation="MIAGIGLGFLLRNHKIHFIQGFIITLIWLLLFLLGLEIGSNKDL
                     VSQFGKLGLEAFILATAGTLGSVFFAKLLWNFIQKKQRNKE"
     gene            8241..9767
                     /gene="gpmI"
                     /locus_tag="PKH68_01225"
     CDS             8241..9767
                     /gene="gpmI"
                     /locus_tag="PKH68_01225"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNX11585.1"
                     /translation="MRKKALLMILDGWGIGNHSRADIISSTPTPYWDSLLATYPNTYL
                     LASGENVGLPDGQMGNSEVGHLNIGAGRIVYQDLVKINRACRDNSILQNPEIVNAYSY
                     ARDNKKKIHFLGLVSDGGVHSSLDHLLKLIDIAKEYGVADAYVHCFMDGRDTDPKSGK
                     GFIEQLQNHTEKSTGKIASIVGRYYAMDRDKRWERVKIAYDLLVNGIGTPATDMVAAM
                     QTSYDDGVTDEFIKPIVAVDADGKPLAKIEAGDVVIFFNYRNDRAKELTIVLTQEDMH
                     EQGMHTIPLEYYCMTPYDSTFKGLHVLYDKDNVENTLGEYVSTLGLKQLRIAETEKFA
                     HVTFFFSGGREAEFPGEERILVPSPKVATYDLQPEMSAPEVADKLVAALNTQKFDFIA
                     LNFANGDMVGHTGVREAIVKAVETIDVCVSKVVEAAKANDYEVIIIADHGNADNAENE
                     DGSPNTAHSLNPVPFVYVTENKNAKVENGILADVAPSICQILGIEKPKEMTGKGLLTF
                     "
     gene            9863..13153
                     /locus_tag="PKH68_01230"
     CDS             9863..13153
                     /locus_tag="PKH68_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNX11586.1"
                     /translation="MNLRTELQSRILILDGAMGTMIQRHKLTEKEYRGQRFADVGILQ
                     KGNNDLLTLTQPDIIQSIHAAYLEAGADIIETNTFNAQRISMDDYGMGAYVHEINSTA
                     VKLARKVADEYTAANPSKPRFVAGSVGPTNKTASMSPDVNNPAFRAVSYDDLVEAYEE
                     QITALIEGGVDALLIETIFDTLNAKAAIFAAEKVMEKLGKRVELMLSATVADVSGRTL
                     SGQTIGAFLTSVSHANLLSVGLNCSFGAKDLKPYLQEISSRAPVYVSAYPNAGLPNQF
                     GEYDETPGKMALQVKEYIDEQLVNIIGGCCGTTPDHIAEYCKLIKDANVRLPKSKSND
                     LHLSGLEELVIKSFTSPLTPLPGERGTKTWQEGMKNANMTTDAMSWKYLIDNAKRMRS
                     EPTSAEKLLWKELRDKRIGYKIRRQHIIDGFIVDFLCLEKNVVIEVDGGIHIGREQRE
                     YDEERTRLLNIKGFQVIRITNDEVESNSFEAAQKIRKELDEYPLRQPNDSDDTHAIFP
                     SPRGEGAGGEVITPETSVEGIDKQEQSLFLNIGERCNVAGSRMFLRLINEKKYEEALS
                     IARNQVDDGAQVIDINMDDAMLDAKQEMTTFLNYIASEPEISRVPVMIDSSKWEVIEA
                     GLKCVQGKSIVNSISLKEGEEDFLSKARKIRQYGAAAVVMAFDEKGQADSFERKKEIC
                     SRAYKLLTEKADFPPQDIIFDPNVLAIATGIEEHNNYGVDFINTIRWIKQNLPYAKVS
                     GGVSNLSFSFRGNNPVREAIHSAFLYHAIQAGMDMGIVNAGMLQIYEDIPADMLQHVE
                     DIVLNRRQDATERMIEFAEKVKNDTSGGKQVQVDAWRSESLQKRLEYALIKGVSDYLE
                     DDLAEALTVYPTAIDIIEKPLMSGMNIVGELFGSGKMFLPQVVKTARTMKKAVAIIQP
                     EIEKQKVPGQSSSAGKFLIATVKGDVHDIGKNIVAVILACNNFEVIDLGVMTPTEKII
                     QTAIDEKVDIVGLSGLITPSLEEMCNVAAEMEKAGLKIPLLIGGATTSKLHTAVKINP
                     NYSGPVVYVKDASVNTSVVANLMNEKTKDVYISAIEDEYQILRDKQVSKTELLSLDDA
                     KSRKPKLF"
     assembly_gap    13295..13394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13467..14819)
                     /gene="hisS"
                     /locus_tag="PKH68_01235"
     CDS             complement(13467..14819)
                     /gene="hisS"
                     /locus_tag="PKH68_01235"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNX11587.1"
                     /translation="MQSPSIPKGTRDFTPREMANRNYIFNTIKDVFRLYGFQQIETPA
                     MENLSTLMGKYGEEGDKLLFKILNSGDFLSGVDDKELNDKNSTKLTNKISEKGLRYDL
                     TVPFARFVVQHRNEISFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVVGSNSLLNEL
                     ELIQIVDEVYRRLKINVVMKINNRKILSGIAEIVGETERITDITVAIDKMDKIGLENV
                     NKEMAEKGISAENIAKIQPILKLSGTNQEKLDALKIILAGSEIGLKGVEELETIFNLC
                     ESVKIKTVVELDLTLARGLNYYTGAIFEVKAQDVQIGSITGGGRYDNLTGVFGMEGVS
                     GVGISFGADRIYDVLNQLELYPETSAEQTQILFVSFGEKELAYCLPWANELRSRGINV
                     EIYPEPAKMKKQMSYADSKGIPFVAIVGETEMLANKVMLKDMKTGEQKLVTIEEIEAC
                     "
     gene            complement(14916..15191)
                     /gene="rpsO"
                     /locus_tag="PKH68_01240"
     CDS             complement(14916..15191)
                     /gene="rpsO"
                     /locus_tag="PKH68_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNX11588.1"
                     /translation="MYLTSEKKQELFKTHGKLKSATDTGSPESQIALFTYRINHLTEH
                     LKVNKKDFSTERALTMLVGKRRRLLDYAKKKDINRYRSIIKELGIRK"
     gene            complement(15295..15957)
                     /locus_tag="PKH68_01245"
     CDS             complement(15295..15957)
                     /locus_tag="PKH68_01245"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX11589.1"
                     /translation="MKRDNYKYIQYFLVPYLTFALGLLLLILFVPKEELHLMMTRHRS
                     VAADWFFRIWTEVGGIIPFIIIIFILFYRYSAAFFLAASQLTGLLIALIVKNIFHAPR
                     PSLYFKEFYPEILLPTVPGFDLMTYNSFPSGHTVNAFALFFCLALITKNKWIKFLCFV
                     AAALVGYSRIYLSQHFAIDVLPGSLIGVISAWMVYPYYLKLHRKWQKHSLADYFIRKN
                     RN"
     gene            complement(15954..16622)
                     /gene="mnmD"
                     /locus_tag="PKH68_01250"
     CDS             complement(15954..16622)
                     /gene="mnmD"
                     /locus_tag="PKH68_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNX11590.1"
                     /translation="MQKPIIKTTADGSNTLYVPEIDESYHSTNGAVQESLHIFINDGF
                     RQCKKETIKVLEIGFGTGLNAFLTLIEAEKENKSVIYTGLEFYPVETEEAVKLNYPEL
                     IEADKRAEFEALHRCEWNREVKISPDFTLIKLKVDFTKTEFSEKYDIIYFDAFSPEKQ
                     PEMWSEKIFEKLYLCAGENAILTTYCAKGIVRRALQSAGFVVERLPGPIGKREILRAR
                     KPIL"
     gene            complement(16715..17293)
                     /locus_tag="PKH68_01255"
     CDS             complement(16715..17293)
                     /locus_tag="PKH68_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNX11591.1"
                     /translation="MMKDLKGTQTEQNLLKSFAGESQARSRYTMFASIAKKEGFEQIA
                     AIFLETAEQELTHAKKFFRYLQGGMVEITAAYPAGVEGTTAENLKAAADGENEEWVDL
                     YPHFADIAEAEGFDNIAATWRKIAKVEAEHEKRYRKLQERVETNTVFAREEETTWQCR
                     ECGHIHTGKEAPKACPVCAHPQAFFEEEKHNY"
     gene            17623..18021
                     /locus_tag="PKH68_01260"
     CDS             17623..18021
                     /locus_tag="PKH68_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNX11592.1"
                     /translation="MIKQIKLTEIILYVSDQARSTRFYQDIFRMPPDLNVPGMTEFNL
                     SDQCKLGLMPDKGIAKILGDKMPHPTNGKGIPRCELYLYVDDVKFEYENALKCGATLI
                     SPPANRDWGDSVCYFSDPDGHIIAFATKCQ"
     gene            complement(18053..20065)
                     /locus_tag="PKH68_01265"
     CDS             complement(18053..20065)
                     /locus_tag="PKH68_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX11593.1"
                     /translation="MKKSFAFLLILLSSFTVFSQSKEQSVHLNEIEVSAEKTNLYTGI
                     SRVVTVIDQQEIQKMPVKSIDELLDNVAGIDVRQRGVNGVQADISIRGGSFDQILILL
                     NGINITDPQTGHYNLDIPLELSDVSRIEILQGSAARVLGPNAFSGVINIVTGERHSVT
                     EPGKNAPETWINDSVDKEKRRNLTEKTSEYSITSQHAVGSYGYLSQSISGNYGDKKWS
                     VFGSAVTKKSDGYIDNTDFDISNIYLQSRINAGNAGIIEVQTAVQLKSFGSNGFYSLV
                     YPNQFEHTKTFLASISWNRQIGAVYLSAQGYWREHHDRFELFRDMEGTEKFTWYTGHN
                     FHLTDITGGKITGSSVWAIGKTTLGVDIRNEHIFSNVLGVKMETPKPVPFEKNKKYTF
                     EDNRLITNIFLDHLIRMDKWTFSGGAALSHAKQFDTHVNGGADIHYSLSKDIRIYASA
                     NSAVRLPTFTDLYYKGAQQIANPNLRPEKSQTYELGSKINSGNLTASASVYYRLGRDV
                     IDWVKRDSTDKKYVSLNLTSVNALGTDLNAQYRLKNGFIRSVTLTYSYLHLYKDVDVL
                     ESKYALDYLKHKLVAGLQHSIYSRLSASWNVGYFDRSGDYIDFVSKQKTAYKPYYLAD
                     VRFIWKTPAIDIYADIYNLTNQPQVDFGGLSLPGRNIVAGVKFRVM"
     gene            complement(20077..20442)
                     /locus_tag="PKH68_01270"
     CDS             complement(20077..20442)
                     /locus_tag="PKH68_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11594.1"
                     /translation="MNKSAAKNKYSSIRFRRWSRKNFAVFSGLHREISIGHIAFNISD
                     KALLKSNKPAFANFTVNSETVSTEEEEKEICNPLLSELSEILIIANSTIDHLAGRSQK
                     YYSHFKQLSIQVNPDIQLF"
     gene            complement(20470..21840)
                     /locus_tag="PKH68_01275"
     CDS             complement(20470..21840)
                     /locus_tag="PKH68_01275"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HNX11595.1"
                     /translation="MKVFQHICLLFLLSLTTFFISCNTTATPVSVSDDATVKAMYFQS
                     NDSSPNLSKGVFIVDHEKGLIYNPDSLPYKTRVDSAYAVFSFASTDGYLINDTFPETS
                     YITSKSYNYSKPVKITNYASDKKSKKEYTIIVNVHKVETYLHVWKNPVSNITEDATDN
                     QKAVLLNNKYFFFTGNGSTTRLYSSADASTWSPESTPAGLPAGATFRNMIVYNNQILL
                     LHNGNELYTSKNALTWEKHLLIGDNNYDFLVLLFNFKNQYWSVAQNKTTKRLNIASSQ
                     DGITWSVQNALSTRFPVSDFAATTFTPRLGREKVIIVGGKDADGKKLNTRWSAENDPT
                     TDSLHWVNLNNSSSKFDAVSGAAIAYYGSKLILIGGADTYDQLRDTSFQMRQSIDEGL
                     TWAKPDTTQNKLPASFKYRTNSSLIHNKTNNTLYIIGGKSILMPFSDVWTVKANFYNF
                     VDPSKY"
     gene            complement(21998..22759)
                     /locus_tag="PKH68_01280"
     CDS             complement(21998..22759)
                     /locus_tag="PKH68_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNX11596.1"
                     /translation="MRIISYNVNGIRAALSKGFEEWLQEASPDVLCLQETKAQPDQID
                     TEIFNRMGYTCYIHSAQKKGYSGVAIFTKISPDNVVVGMDNHKYDSEGRVLRADFDDL
                     TVVCVYIPSGSSGGERQQFKMGFLADFHEFISDLRKTRSNIVVCGDYNICHKPIDINH
                     PERQTGVSGFLPEERAWLDEFESAGMVDSFREFDKSADKYSWWSFRTAARSRNMGWRI
                     DYHWVSHPLKPRLKSAAILADAVHSDHCPVLVELN"
     gene            complement(22861..23619)
                     /locus_tag="PKH68_01285"
     CDS             complement(22861..23619)
                     /locus_tag="PKH68_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX11597.1"
                     /translation="MIKVTNLFKSFDGEEVLKDISAVFETGKCNMIIGQSGSGKTVLM
                     KSIIGLHRIDSGRIEYDGRNMPDMRMRDIRLLRREVGMLFQGSALFDSLTVMENVMYP
                     LEMFSSSTKKEMELRAKFCLDRVNLPEHSYHLFPSEISGGMQKRVAIARAISLNPKYL
                     FCDEPNSGLDPKTSIIIDKLIFEITHEFNITTIVNSHDMNSVIEIGENIIFINNGLMA
                     WSGTNKDIYGAKNEDLNDFVFASELYKQVKMINQ"
     gene            complement(23629..24369)
                     /locus_tag="PKH68_01290"
     CDS             complement(23629..24369)
                     /locus_tag="PKH68_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX11598.1"
                     /translation="MSIIKDTGQYALLMYRVFAVPDRGRMFWRQFPKELEKLGLASLP
                     IVIIISVFIGAIMTIQTKLNTENPLLPTYSTGLVTRDTLLLEFSSTIMCLILAGKVGS
                     NIASEIGTMRITEQIDAIEIMGVNSANYLILPKITAFVVMMPFLVIFSMAIGLFGGYL
                     VGVFGDIITTADYLLGIQYAFIPYYVFYSVVKSLVFAFLISSVASYYGYYAYGGALNV
                     GKASTNAVVNSSILILFFNLLITNLMLN"
     gene            24783..26414
                     /locus_tag="PKH68_01295"
     CDS             24783..26414
                     /locus_tag="PKH68_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HNX11599.1"
                     /translation="MIKNLTITALLVLATSSLFAGGLLTNTNQSVHFLRNPARDASTE
                     IDAVYTNPAGVVFLSDGFHISLNNQSAFQTRTITSTFAPFAGYGGSATKEYGAKTNSP
                     VVPSIQAAYKKDRFAISAGFGVVGGGGVAAFNKGLPSLEAPVSMIPLSLTTNKIPTSS
                     YSVDQYMEGKQYTFGVQLNGSYKITEHLSAGVGVRVNLVSNAYAGHLTNIMIAPNQPA
                     FGASYNGSTLVSAPKFFTDAATTIRGWATGATSYVAGLQPIVSGGGGTVLLSNGTAAG
                     LSAAQIAQIQGLLGAAGLTPAQIGAINIQTAQGTLAAAAPVFTGKADAMTASASGTAD
                     KQLDTKQSGMGFTPIFSLNYNTDRVNVAVKYEMKSTITLTNKTTVDNTGMYADGAKTP
                     NDIPAYLAAGADFKLTDNWKVSGGYHHFFDSDAKMANDKQQYINGGINEYLLGTEYKF
                     GKRFLLSAGGQLTRTAVTDDYQSDLSFSLHSVSVGFGGAVDLTENLRLNLACLLTNYS
                     DWTKTSTNYNKTNLTGTDVYARTGKTFGIGIDYRF"
BASE COUNT         7729 a         5356 c         5607 g         7690 t
ORIGIN      
        1 ctgcaccggt taccaacttt gcactggcgg gtatgtcggg actcatgtcg ggtgttatgc
       61 atgcacctct gacaggtatt tttcttattg ccgagcttac cggaagttat aacttgttca
      121 tgacgctcat gatagtttcg gtggtttcgt ttctgacaat cataatattc gagccacaca
      181 gtttgtatgc catgcgtctg gctcaaaaag gagaattgat tacacacaac aaagaccggg
      241 cagtgctcac acttctgaaa atggaaaatg taatagaaac tgatcttacg gaactcactc
      301 ctactatgac gctcggtcag ctcgtgacgg tcatttcccg ctccaaacgc aatatttttc
      361 cggttgttga gccccaaaac cggattttga tgggtgtggt tttgttggat gaagtgagga
      421 atataatgtt tcaccccgaa cattacaacc gctttaccgt ggcacagttg atgatttcgc
      481 ctccggcaat aattaacgtc aatatgacaa tggaaaaagt aatgcagata tttgaggact
      541 ccaaggcctg gaatctaccg gtagtcgatg atcagaaaaa gtacctggga tttgtttcaa
      601 aatcgaaaat attcaattca taccggcatg tattggtgca tttttcggaa gaataataat
      661 aaaatgatat gggaaaaaaa ataatgtata gcgatgctgt agccgaatta acaaaaattc
      721 tggccgacct tgaaagttcc ggttctgaaa tcaatatgga attgatagag gaaaaagtga
      781 aacgtgcagc ttacctgctc gatttgtgta aaaaacaatt gcatgaaatt aacacggagc
      841 tcgaagtcat tcttgaaaat ctggagtgaa atcagatcag cataatccct gaaatcatcc
      901 tgcccgaaga aattcccaaa cgacgggctg aaaaaaagtt ttcatgctgg ctataggtgc
      961 aaatacccga aatttcaata ttttcctctt tcacgccacc atcagtcagc atctgtttat
     1021 tggctttcca cagatcgaga aaaattcgtc cgtttttctc tgagaatatt tccttcgttt
     1081 caaatcccgc ttccctaaat tcatccataa gaggagcacc cacttcatag gcatcgggcg
     1141 atatggatgg tcctaatacg gctaccaggt tggctgggtc ggtttcaaaa gcagatttca
     1201 tggctgtaat tgtttttgtg acaattccgg agcaggtacc tctccagcca gcatgtactg
     1261 caccaattgc tttacacacc ggatcatata gcaggatggg gacacaatcg gctgtagaaa
     1321 cacctataca ttgaccggga atgtcagtaa tcagggcgtc aattccgtgt aaaagtgact
     1381 tttttgtttc atcatgctgg ccgggaaaat tttcaccaat ttgaaatatc cgattgctgt
     1441 gggtttgaaa gggaaggtgt atggtatttt tatcaattcc agtcaggctg ccaagcaggt
     1501 cgaaattttg agagatgttt tcgggtaaat cgctggtgaa taatcccaaa ttgaacgagg
     1561 aataatttcc aacactaaca ccaccaatac gggtggtggt gaaatgccgg atgctgtcaa
     1621 accgcttcag gatttggtac tggattaaac agggatttgt attactgctc atcccatcca
     1681 atgcccttgt acttacttaa atcatcctcg tcatcaaatt catccacctc tatttccatt
     1741 tcatcttctt cggtaagcat ttcggctact tctatcggtt tgtgtaccat ttctaccaat
     1801 ttattggtct ctttgttcag ttcttcccat atcatgtctt tcagttcact gatgccggcg
     1861 ccgctcaccg aggaaataaa caatgttttc acatcatcgg gcaaatcttt tttcatttgt
     1921 tcaatcagct cttcatccag catatcgcat ttcgaaatgg ccaatatgcg ctgtttgtca
     1981 atcagttcgg ggttgtattt ctgaagttcg ttgagcagaa tgttatattc atttttaata
     2041 tcgctggcat cggcgggaac cataaaaagt aaaattgaat ttcgctcaat gtgtcgcaaa
     2101 aatctcaatc ccaatccttt tccttccgaa gcgccctcaa ttatccccgg gatatcggcc
     2161 ataacaaacg atttattgtc ccgataggcc acaatcccca aattgggggt aagcgtggta
     2221 aacggataat cggctatctc cggcttggca gccgaaacaa cggatagcaa tgtggatttt
     2281 ccggcatttg gaaatcctac caaacccaca tcggccagta tcttgagttg taaaaccacc
     2341 gttttttcca cacggggttc tccgggctga gcataacggg gagcctgatt ggtggcggta
     2401 cggaaatgcc agttgcccaa gccgccacga ccacctttca ttagtattac ctcctcgccg
     2461 tggttggtta tatcgcacat aaattcacct gtttcgccat cgtaaaccac tgtgccacac
     2521 ggaacttcga ttaactgatc tttgccatcc ttaccgaagc tgcggctttt ggagccgtcg
     2581 ccaccatcac cggcataaat gtgtcgtgaa tatttcagat gcaacagggt ccacaggtta
     2641 ctgtttcccc ggagtatgat gtgtccccca cgaccaccat caccaccgtc agggccacct
     2701 ttggtgatga atttttcgcg gtgaaaatgc aacgagccag cgccaccttt acccgagcga
     2761 caaaatattt taacgtaatc aatgaaattc gatcctgcca taatttgttt ttatattatc
     2821 agaaataaaa gagaaaaatt ataaagttag tttttttaga aaaaataaat ccattgcaga
     2881 atttcacaca actcatcaat ggatttttga tataaatata acaagctgga cctgttaaag
     2941 tttttcgtct attgcggccg aaattcgccc gaatatttca tcgatagtac ctattccatc
     3001 aatttcaacg tatttaccca gcgatttata atactcactt actggttttg tctgtaaata
     3061 gtacacatcg agccgttttt tgatggtttc catattgtca tcgcttcgtc ccgatgtttc
     3121 accgcgcagc agcaggcggg tgataagttc accctcatct acctgtaagt ccagcaggat
     3181 atcggtttga gtaccacgtt tttggaacag aatttcgaga gcttcggcct gcggcagagt
     3241 tcgcggaaat ccatccagta tgatgccgtt cgtttggctg tgattgagtg tgtcaatttt
     3301 acgggccagc atttctataa tcatttcatc cggaacgagt tgtccttgtg aaatgtattg
     3361 atgtgccaca gtgccaagtt ctgttttgtc agaaatttct ttgcggagca aatctccggt
     3421 agaaatgtgg gtgagattgt atttttttac aattaaatcg ctttgagtgc ctttaccgca
     3481 tccgggagca ccacagatga tgatattgat catagaaatc aggtttatag aattaaaaaa
     3541 atgatgaagt aatatcaatc aattactgta tagatatctt tcagattgcg tccgtaaccg
     3601 tcgtagtcaa ggccataacc cacgatgaat tcgtttggaa ttttcatggc tacataatca
     3661 acacgtacat cgcgcctgag agcagccggc ttttgtagga aggtggccac ataaatttcg
     3721 ttagcacctt tggcctgcag ggaagtgaga attttatcca tcgtaatgcc ggtatccact
     3781 atatcttcca ctaccaccac attgcgtccg actacacttt cgttcagccc catcacttct
     3841 tttactgtcc cggttgtgaa gagaccttcg tatgaagaaa gtttaatgaa agtgatttca
     3901 cagggtatgt caactacttt catcaaatca cccgcaaaca taaatgcacc gttcagtaca
     3961 cagataaata gaggattttt gtctttcaaa tcccggttga tggcatcccc aacggtttgt
     4021 actgctttca gaatatcttc ggatggaatt gataatgtga attgtttatc tttgatttgg
     4081 attgttttca ttcgcttttt gtttgaggat aaattaatcg tcaaaattaa aaaaaaaata
     4141 ttaatcagca gtattttgga gccaataatc tcattaaata tcccgatttg tttttctgcc
     4201 atcaaaaaaa ctaaaaaact tcggtatagt tttatttttt aaaaataatc agtacatttg
     4261 ctaactaaat atatacgatc aatcaaccaa aaataccaag gatatgaaat ttgttgtatc
     4321 aagcacggaa ttattaagcc atttacaagc tatcagtcgg gtgattaaca gtaaaaactc
     4381 gttacagata ttagataatt ttctgttgag tctggaaggc aataccctca ctatgactgc
     4441 ttcagatata gaaacaacac taataacctc tatggaggtg caggatgtgg aaggcagtgg
     4501 aaaagtggct gttgctaaca aattattgct tgatacgctg cgtgaattct ccgaacaacc
     4561 gctgagcttt tacattgacg acagcaacct ggctatggta attacctcct ccaacggtac
     4621 atacaatttt atcggtcaga gtggcgatat gtatcctcag ttgcctttgc tcgaagataa
     4681 cgcccgtcag ttgaacattt ctgtgcctgt attattcagc ggaatttcaa aaacattttt
     4741 ttgcatggcc gatgacgaac tgcgaccggt aatgaatgga atatttttcg atattgctcc
     4801 tgacagcctt accctcgttg ccacagatgc tcataaactc gtgcgtttca agtccgctta
     4861 tttgtcagca tccattaagg aagacgaaac attcaacttt attcttccaa aaaaaccggc
     4921 tatcatgctt cgcaatattt tgcccaaaga gtcaggcgaa gtgaatatac attttgatgc
     4981 caaaaatgct tatttcaagt tgagcaatta taccatggtt tgccgtcagg tggaaggccg
     5041 gtatccgaac tacaacggcg taattcccaa aaacaatccg tacaaggtta tagccgatcg
     5101 ggtcacactg ctcaatgcgc tgaaacgtgt ttcggtgttt tccaatcagg ccagtaatct
     5161 cataaaactt gttttcaatc aaaatcagat acatgtatcg gctcaggata ttgacttttc
     5221 catatcggcc gaagaataca ttccctgcca gttcgaaggc gaagaaatta aaatcggctt
     5281 caaatccact ttcctcattg aaatgctggg taatgtagat gccagcgata ttgtcatcga
     5341 acttgccgat ccatcgcgtg ccggcatcat actgcctttc gaaaatgcag aaaatgagga
     5401 tattctgatg ctgctgatgc cgatgctttt aaatgactaa tgtgaaataa aataatcttt
     5461 gtagggcaga aaaacatgga agacgtgttt ttctgctttt ttttaatgaa actgaactta
     5521 taaaaaccga taaataatga aactcaacct gaaaaatcca attgtttttt ttgatctcga
     5581 aactacgggt accaatattg tgtccgacag aattgtcgaa atttcgtacc tgaaaatttc
     5641 tcccaacgga agagaggaaa gtaaaacgat aaggattaac ccggaaatgc atattcccga
     5701 aagttcttca gccattcacg gcatttacga ccaggatgtg gccgactgtc ccacattcag
     5761 ggaagtggcc aaagaaataa tacacgatat agaaggttgt gacctggcag gttacaattc
     5821 aaatcggttc gatattccgc tgctggcaga ggaattgtta cgcgctgatg tggatattga
     5881 ccttatgaag aggaaatttg tggatgttca ggttgttttt cataaaatgg aacaacgcac
     5941 actttcggct gcctacaaat tttattgcga aaaagacctg gaaaatgcac acagtgctga
     6001 ggccgataca atggcaacct atgaggtgct tcaggctcag ctggacaaat acccggagct
     6061 taaaaatgat attgaatttt tgtcaaaatt tacagcacaa accaataacg tggattttgc
     6121 aggaagaatt atttacaatg aaaaagggga ggaaatcatt aactttggca aatataaagg
     6181 tcagaaagtt accgatgtct tgaaaacaga cattggatac tacggatgga taatgaatag
     6241 cgatttcacg ctccacacca aaaaagtgct gaccaatatc aaacttcgtg attttaataa
     6301 ataaaaaaat gaccattaat taataaatca ggttaaaaat catattttca gtcagttatt
     6361 tacattgtgg cataatcatt gaggcatact atgtgtaagt aactgtgatt aaatgaagtg
     6421 tcatgagacg tcttaaattc atcctatttg ttctgttagc tgcaatctgc ttcccggtag
     6481 tcattacagc ccagactgtc tctgtcaccg atcgaacacc cgagcaggaa gccatcaaac
     6541 aaaccgaaaa acttcaaagt gagctcaaac tgactgacga acaggcaaag atcgtttatg
     6601 aaatcaatct taggtatgcc cgtgaacggc agttgtccaa ttccagaagt caggcaatgg
     6661 aacgtataaa aaataaaaat gaagaaattc agcgagtact ctccccgcag caatatgatg
     6721 aacttctgta tcggaaaaac gatgttcaaa ctgtggaaat cgataatgcc aaacgctata
     6781 ttcacaccaa tccgcaaaac cgggtgaaca ataccgatgt gaaaagtccg gcactgagaa
     6841 caagaaattc agatccgggc gtggaagatc agcgttcact gagaatggga atacggcaaa
     6901 atgaatctgt agcacaaccc cgaacaggca gatcggttga aactaattca cgttccgtgg
     6961 ttcccacaag gtcgtcggag actgttacta cacgcagttc atcgggtcgt acatcagaga
     7021 gcggctcatc tacccgtcct gccacacgac gttaacttat ttagtctgta ttcatgtcca
     7081 atacctggtt taagttcaag caatttacag tcagtcacga caaatgtgcg atgaaagtgg
     7141 gtgtggatgg agttacactt ggagtatggg cggatgttgc tgaggcaaag aaggtgctgg
     7201 atgtaggttg tggttccgga ttgatttcgc tgatgattgc tcagcgttgt gatgctcaga
     7261 ttacagctat tgatattgat aatcagagca ttattcaaac cgctgaaaat gtggataatt
     7321 caccgtggaa agacagaatt aaaactcagc ctatttcgtt gcaggaatat gcacgtgtaa
     7381 cctccgacag atttgatgtc attatttcaa atcctccttt cttcaataat tcccttaaaa
     7441 atccatcaga aagtcgaacc accgcccgcc ataccgacag tctatcacac gaagatttgc
     7501 tgatgtgctc ccggaaattg cttcatccgg atggaaagtt atgtctgatt ttacccgtag
     7561 aagaaggaaa tgcctgcatt gaaacgggaa aagaaacagg tttgttttgt tccaaaaaag
     7621 tcagtatttt cccgaattca tcaaaaccgg caaaaaggtt gttgctggaa tttattttat
     7681 ccgaaacagc atgtgtggaa cgaaatctga cgattgaaac cgatgtccga aatcaataca
     7741 cacccgaata taccaatctg gtgaaggatt tttacctgaa attgtaaaga aaaagattgt
     7801 tattccttgt ttcgttgctt tttctgaatg aaattccaaa gaagttttgc aaaaaaaaca
     7861 cttcccaacg ttccggctgt tgccagtata aatgcttcca ggccgagttt tccaaattgt
     7921 gatactaaat ctttattcga acctatttcc agtccgagca agaacaataa caaccagata
     7981 agcgttatta taaatccttg aataaaatgg attttgtggt ttcggagtag aaaacccaat
     8041 ccaattcctg ctatcataca tgcaatgact ataaacattc gtttttttga gcaaagataa
     8101 aataaataag actaccgaag taaaaaacag tttaaaactt aaaaaatcgg gatgcgaaat
     8161 gctgaaattt aagtaatttt gcagtcgcaa acgcaaagca gataaagatt tattaatcag
     8221 aataataata acaataaaat atgagaaaaa aagcattatt aatgattttg gatggttggg
     8281 gaataggtaa tcattccaga gccgatatca tttcaagtac accgacaccc tattgggaca
     8341 gtttattagc aacatatccg aacacatacc tgctggcttc cggcgaaaat gtaggtttgc
     8401 ctgacggaca aatgggtaat tcggaagtag gtcacctgaa tattggtgcc ggacgcattg
     8461 tttatcagga tttggtgaaa ataaaccgcg cctgccgtga caacagcatc ctgcaaaatc
     8521 ccgaaatagt gaacgcatat tcctatgccc gcgataacaa gaaaaaaatt cattttctcg
     8581 gtttggtttc cgatggtggt gtccacagtt cgctggatca tttgctgaaa ctgattgata
     8641 ttgccaaaga atacggagtt gcagatgcgt acgttcattg ttttatggac ggacgtgata
     8701 cagacccgaa aagcggtaaa ggctttatcg aacagttgca gaaccatacc gaaaaatcaa
     8761 ccggaaaaat cgcttccatc gttggccggt actatgccat ggaccgtgac aaacgctggg
     8821 aacgggtgaa aatagcttac gatttactgg tgaatggtat tggaactcct gccacagata
     8881 tggtagctgc tatgcaaact tcatacgatg atggcgttac cgatgaattt attaaaccaa
     8941 ttgtggcggt tgatgctgac ggaaaaccac tggcaaaaat tgaagcaggc gatgtggtga
     9001 ttttcttcaa ctatcgcaat gaccgtgcta aggaactgac cattgtttta actcaggaag
     9061 atatgcacga acagggaatg cacaccattc cactggaata ttactgtatg acgccatacg
     9121 acagcacttt caaaggttta catgtgttgt acgataaaga caatgtggag aataccctgg
     9181 gagaatatgt ttcaactttg gggttaaagc aattacgtat tgccgaaact gaaaaatttg
     9241 cacacgttac ctttttcttt tccggtggcc gtgaggctga atttccgggt gaagaacgta
     9301 tactggttcc atcccccaag gtggcaacct atgatttaca gcccgaaatg agtgcacccg
     9361 aagtagctga caagctggtg gctgccctga atacacagaa atttgatttt attgccctca
     9421 attttgccaa tggcgatatg gtcggtcata ccggcgttcg cgaagccatt gtaaaagctg
     9481 tggaaaccat tgatgtttgc gtaagtaagg ttgtggaagc agccaaagcc aatgattatg
     9541 aggtgattat cattgccgac catggtaatg ctgataatgc agaaaatgaa gatggttccc
     9601 caaatacagc acactcactt aatccggtgc cttttgtata tgtaactgaa aacaaaaatg
     9661 caaaggttga aaacggtatc ctggctgacg tagctccttc tatctgtcag attttaggta
     9721 ttgagaagcc aaaagagatg accggtaaag gtttgctgac tttttaacga cataaaataa
     9781 aatgtaaaaa cgctgtaagt caaaacttac ggcgtttttt tatggataga aagcactatc
     9841 tttgcatatc aaaacaacaa ttatgaactt acgtacagaa cttcaaagcc gcattctgat
     9901 attggatggt gcaatgggta cgatgatcca gcgtcataaa ctcaccgaaa aagagtatcg
     9961 cggtcagcga tttgccgatg tcggaatttt acaaaaaggg aataacgatt tgctcacgct
    10021 gacacagccg gatattattc agtctattca tgcagcctat ctggaagctg gtgctgatat
    10081 tattgaaacc aatacattca atgctcagcg tatttcgatg gatgattacg gaatgggagc
    10141 gtacgttcac gaaataaatt caaccgctgt aaaacttgcc cgaaaagttg ccgatgagta
    10201 tactgctgcc aatccttcaa aaccacgatt tgtagccggt tcggtagggc caaccaataa
    10261 aacggcttcc atgtcgcccg atgtgaataa tccggctttt cgcgcggtaa gttacgatga
    10321 tttggtcgaa gcatacgaag aacaaatcac ggctttgatt gaaggtgggg tagatgcttt
    10381 actgattgaa accatttttg atacgctgaa tgcaaaagcg gccatttttg cagctgaaaa
    10441 agtaatggaa aaacttggga aacgggtaga actgatgctt tccgccacgg tggcggatgt
    10501 gagcggccgt actttatccg gtcagactat cggagctttt ctcacttccg tttcacatgc
    10561 caatttattg tctgttgggc ttaactgttc atttggagca aaggatttga aaccctattt
    10621 gcaggaaata agcagccgtg caccggttta tgtcagcgct tatcccaatg ccggtttacc
    10681 caatcagttt ggcgaatatg atgagacacc gggaaaaatg gctcttcagg taaaagaata
    10741 tattgatgag caactggtaa atattattgg tggttgttgc ggaaccacgc ccgatcatat
    10801 agcagaatac tgtaaattaa ttaaagatgc aaatgtacgt ttgccaaaaa gtaaaagtaa
    10861 tgatctgcat ttgagcggat tggaggaatt ggtgataaag agtttcacct cacccctaac
    10921 ccctctcccc ggggagaggg gaaccaaaac ctggcaggaa ggaatgaaaa atgcaaacat
    10981 gacaacagac gcaatgtcat ggaagtatct gattgacaat gcaaaaagga tgagaagtga
    11041 acctaccagt gcagaaaagt tactttggaa agaactcaga gataaacgta ttggctacaa
    11101 aatcagaaga caacatatca ttgatggttt tattgttgat tttttatgtt tggaaaaaaa
    11161 cgtagtcatt gaggttgatg gtggaataca tataggaaga gaacagcgtg aatacgacga
    11221 ggaacgaact cgtttattga atattaaagg ttttcaggta attcgtataa caaatgacga
    11281 agtggaaagt aattcttttg aggctgccca aaaaatcagg aaagaattag atgaatatcc
    11341 tttaagacaa ccaaatgata gtgatgatac gcatgccatt ttcccctctc cccggggaga
    11401 gggggcaggg ggtgaggtca taactcccga aacaagcgta gaaggtattg ataaacagga
    11461 acaatccctc ttcctcaaca tcggtgaacg atgcaatgta gccggttcac gcatgttcct
    11521 gcgtttgatc aatgaaaaaa aatacgaaga agctctttcg attgcacgta atcaggtaga
    11581 cgacggtgca caggtcattg atatcaatat ggacgacgcg atgctggatg caaagcagga
    11641 aatgactact tttctgaatt atattgcttc ggaacctgaa atttctcgcg tgccggttat
    11701 gattgacagt tcaaaatggg aagtaataga agcgggactg aaatgtgtac agggaaaaag
    11761 tattgtcaat tccatcagtt tgaaagaagg tgaagaggat tttttgtcca aagcccgtaa
    11821 aataagacag tatggtgctg ctgcggttgt gatggcattt gatgaaaaag ggcaggctga
    11881 cagttttgaa cggaaaaagg aaatttgcag tcgtgcatac aaattgctga ccgaaaaagc
    11941 cgattttcca cctcaggaca taatttttga cccgaatgta cttgccattg ccaccggcat
    12001 tgaagaacac aataactacg gtgttgactt tatcaacacg atccggtgga taaaacaaaa
    12061 cctgccttat gctaaagtga gcggtggtgt gagcaatctt tctttttctt tccggggaaa
    12121 taatcccgtg cgtgaggcta ttcattccgc atttctttat catgctattc aggccggaat
    12181 ggatatggga attgtcaacg ctggaatgtt gcagatttac gaagacatac cggccgatat
    12241 gctccaacat gtggaagata ttgtgctcaa ccgccggcag gatgctaccg aacggatgat
    12301 agagtttgcc gaaaaggtaa agaatgatac ttccggcgga aaacaggttc aggtagatgc
    12361 ctggcgatct gaatcgttgc agaaaagact ggaatacgct cttataaaag gtgtctccga
    12421 ttatctcgaa gacgatttgg ccgaagcgct gaccgtttat cctactgcaa ttgatattat
    12481 tgaaaaaccg ctgatgagcg gaatgaatat tgtgggtgaa ctgtttggct caggaaagat
    12541 gtttttgccc caggtggtaa aaacagcccg aacgatgaaa aaagcagtcg ctatcattca
    12601 accggaaatt gaaaagcaga aagttcccgg tcaaagttca tcagccggta aatttctgat
    12661 tgctacggtt aaaggagatg tacatgatat agggaaaaat atcgtggctg tcattttagc
    12721 ctgtaataat tttgaggtca ttgacctggg agtgatgaca ccaaccgaaa aaattattca
    12781 gactgccatt gatgaaaaag tggatattgt gggattgagc ggactgatta ctccttcgct
    12841 ggaagaaatg tgtaatgtag ctgctgagat ggaaaaagcc ggattgaaaa ttccgttgct
    12901 aataggcggt gcaactactt ccaaacttca tacggctgta aaaataaatc cgaattacag
    12961 cggaccggtg gtttatgtga aagatgcatc ggttaataca tcggttgttg caaatttgat
    13021 gaatgaaaag accaaagatg tttacatatc ggccatcgag gatgaatatc agattttgcg
    13081 cgataagcag gtgagtaaaa cggaattgct ttcgttggat gacgcaaaaa gtagaaaacc
    13141 caagttgttt taagagtagc ccccaaaatc cccctcaagg gggactttcg gaaagagaga
    13201 agagagccaa tttatagtat tgtataaagg ttgttcttct tcgatagatt attcataaaa
    13261 ttcccccttt agggggttag ggggcttact tcggnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnttcgat agattattca taaaattccc cctttagggg gttagggggc
    13441 ttacttcggg ggatttaggg ggctttttaa caggcttcaa tttcttcaat tgttactagt
    13501 ttttgttctc ccgttttcat gtctttcagc attaccttat tagcaagcat ttcagtttcg
    13561 cccacgatgg ctacgaatgg aattcctttg ctatcggcat agctcatttg ctttttcatt
    13621 ttggcgggtt cggggtagat ttctacgttg ataccccttg aacgaagttc attggcccat
    13681 ggcaggcagt aggcgagttc cttttcaccg aaagaaacaa ataatatctg cgtttgctca
    13741 gcagatgttt cggggtagag ttcaagttgg ttcagcacat cgtaaatacg gtcggctccg
    13801 aatgatatac ccactccaga gacgccttcc atgccgaaaa cacccgtgag attgtcataa
    13861 cgtccgccac cggtgatact gccaatttgt acgtcttgtg cttttacttc aaaaatggca
    13921 ccggtataat aattcagtcc gcgagccagc gtcaaatcaa gctccacaac tgtttttatt
    13981 tttacgcttt cgcaaagatt gaaaatcgtt tccagttctt ccaccccttt cagcccgatt
    14041 tcagaacctg ccagaatgat tttcagtgca tccagttttt cctgattggt cccgctgagt
    14101 ttcagaatgg gttgaatttt agcaatattt tcagcagaaa ttcctttttc agccatctct
    14161 ttattcacat tttccagccc gattttatcc attttgtcaa tggcaacggt tatgtcggta
    14221 atacgttctg tttcgcccac aatttcagca atacccgaca gaattttgcg gttgttgatt
    14281 ttcatcacca cattgatttt caggcgtctg taaacctcat ccacaatctg tatcagttcc
    14341 agttcattca acagcgagtt gctgcccacc acatccacat cgcattggta aaattcgcgg
    14401 taacgtcctt tttgcggacg atcggcacgc cagacgggct gtatctggta gcgtttgaag
    14461 gggaaagaaa tctcattccg gtgttgcacc acaaagcggg caaaaggcac tgtcaggtcg
    14521 taacgcaatc ctttctcaga gattttattt gtcagttttg tgctgttttt atcgttcagc
    14581 tccttgtcat caacacccga aagaaaatca cctgaattca ggattttaaa cagcagttta
    14641 tcaccttctt cgccgtattt ccccatcagc gtggagaggt tttccatcgc cggcgtttct
    14701 atctgttgaa atccgtagag gcgaaaaacg tcttttattg tgttgaaaat atagtttctg
    14761 ttggccattt cacgcggcgt gaaatcccgg gttccctttg gtatggatgg tgattgcata
    14821 ataatcggtt ttgtggaaat atttcagtac aaatataaac taaaaaggca tctcaataga
    14881 aaattgaaac gccttttttg ccttgaaagc aagtattact ttctgatacc taattctttg
    14941 ataatagaac ggtagcgatt gatgtctttt ttcttagcat aatcaagcaa acggcggcgt
    15001 tttcctacca acatggtcag ggcgcgttct gttgagaaat cttttttatt aaccttcaaa
    15061 tgttctgtta aatggttaat gcgataagta aacaaggcaa tttgagattc gggtgaacct
    15121 gtatcggttg ctgattttaa tttaccgtgc gttttgaaaa gttcctgttt tttttctgat
    15181 gttaaataca tactgattaa ctatttgggt tatacattaa gaacagagct ttcactctat
    15241 gttccaataa ttgagggtgc aaagataaga tttttatttt gaattgtcat caggttagtt
    15301 cctgtttttt ctgataaaat aatcggccag cgaatgtttt tgccattttc ggtggagttt
    15361 taaatagtat ggataaacca tccaggcgga aataactccg ataagtgagc cgggaagcac
    15421 atcaatggca aagtgctgcg acaggtagat gcgtgaatag ccaacaagtg ctgctgcaac
    15481 gaaacaaagg aacttaatcc atttattttt cgtaatcagt gccaggcaaa aaaacagggc
    15541 aaaagcattg actgtatgtc ccgaaggaaa actgttgtaa gtcatcagat caaatccggg
    15601 tacggtgggt agtaaaattt cgggataaaa ttccttaaaa taaaggctcg gacgtggtgc
    15661 gtgaaagata ttttttacaa taagagctat cagaagaccg gtcagttgcg aagcagccag
    15721 aaaaaatgca gcgctgtacc ggtaaaaaag gataaatatt atgattatga atggaatgat
    15781 accacccact tctgtccaaa tgcgaaaaaa ccaatcggca gcgaccgaac ggtgcctggt
    15841 catcatcagg tgcaattcct ctttggggac aaagagtatc agcaataata atcccagtgc
    15901 aaaagtcagg taaggcacca gaaaatattg aatatattta taattatctc ttttcataat
    15961 atcggttttc ttgcacgtaa aatttctctt ttcccgattg gtccggggag ccgttccact
    16021 acaaatcctg ccgactgtag ggcacgccgc acaattccct tggcgcaata ggtggtcaga
    16081 attgcatttt caccggcaca aagatacaat ttttcaaaga ttttttccga ccacatttcg
    16141 ggttgctttt ccggagaaaa tgcatcaaaa tatatgatat catatttttc cgaaaattca
    16201 gttttcgtaa aatctacttt caattttatc aacgtaaaat caggactgat tttcacctct
    16261 ctgttccact cacagcggtg taatgcttca aattcagctc ttttgtcagc ctcaatgagt
    16321 tccgggtaat tcagtttaac agcttcttcc gtttctaccg gataaaattc taatccggta
    16381 taaattactg atttattttc tttttcagct tctatcagtg tcagaaatgc attcaggccg
    16441 gtaccaaaac cgatttccag cactttaatc gtctcttttt tacactgacg aaatccatcg
    16501 ttgataaata tatgcagcga ctcctgcaca gctccgttgg tggagtggta actttcgtcg
    16561 atttcgggca cgtacaaagt gtttgagccg tcggcggtgg ttttaataat tggtttctgc
    16621 atacagttgg caaaaataat tctttttcaa caaaaaacta ccgctgctca gcagttgaac
    16681 agcggtagta cctttttttt atccgtgagt tggattaata gttatgtttt tcttcttcga
    16741 aaaatgcctg tggatgagcg caaaccggac aagctttcgg agcttccttg ccggtgtgaa
    16801 tgtgtccaca ttcgcggcac tgccaggttg tttcttcttc gcgggcaaaa acagtattgg
    16861 tttctacgcg ttcttgcaat ttgcggtagc gtttctcatg ttcagcttcc acttttgcaa
    16921 ttttacgcca ggtggcagca atgttgtcaa atccttcggc ctcggctatg tcggcaaagt
    16981 gtggatacag gtcaacccat tcttcgtttt cgccgtcggc agctgctttc agattctcag
    17041 cagtagtacc ttctacaccg gcaggataag cagcagtgat ttcaaccata ccgccttgca
    17101 gataacggaa gaatttcttt gcgtgtgtca gttcctgttc agcagtttcg agaaaaatag
    17161 ctgcaatttg ttcaaaacct tcttttttgg caatgctggc aaacatcgtg taacgactgc
    17221 gagcctgact ttcaccggca aaagatttca gcaggttctg ttcagtctgt gttcctttta
    17281 aatctttcat aatgataaat atttaattta taaatgttag ttgaaataat ttattgcaaa
    17341 tttaatgttt tatttgtgat aaacaaaaat aattctcagg attgtaatcg taatttctgt
    17401 tatatttttt ttgtttcaat aaaataaaaa agtgaaataa accttttata gtacaagaaa
    17461 gatatttatc aaatgtatgt aaatcagacc aatacgtgta tgagtgtgat tttagattgg
    17521 atttctataa tatattctct atttttgtgt ttcacaaata tgggaaatag atgaatgact
    17581 tagaattcca ggttaccacc agaatactaa caagaattta ggatgataaa acaaattaaa
    17641 ttaacagaaa tcatacttta tgtcagcgat caggcgagaa gtacccggtt ttatcaggat
    17701 atttttcgaa tgccacctga tttgaacgtg ccgggtatga cagagtttaa tttatcagat
    17761 caatgcaaac tcggactgat gcctgacaaa ggcattgcta aaattttagg agataaaatg
    17821 cctcatccca ctaatggaaa gggcattccg cgatgcgaac tctatcttta cgttgatgat
    17881 gtgaaattcg agtatgagaa tgcgctgaaa tgcggtgcaa ctctgataag tccaccagcc
    17941 aaccgggatt ggggtgacag cgtgtgctac ttttcagatc ccgacgggca tattattgca
    18001 tttgcaacta agtgtcaata agctatcgga ctgataacgt ggcaaatatg aattacatca
    18061 cccgaaattt tactcctgcc acaatgtttc gacccggcaa tgaaagaccg ccaaagtcaa
    18121 cctgaggttg gttagtcagg ttgtagatat cggcataaat atcgatggcc ggagttttcc
    18181 agatgaacct gacatcggcg aggtaatagg gcttgtaggc ggttttttgt ttgcttacaa
    18241 aatcgatgta atcgcctgaa cggtcaaagt atccaacatt ccagctggcc gatagccgtg
    18301 aatatattga atgctgcaac cctgcaacca atttgtgttt gaggtaatcc aatgcgtatt
    18361 tcgactcgag cacatccaca tccttgtaca aatgcaggta tgaataggtg agcgtaaccg
    18421 agcgaatgaa accatttttt aacctgtatt gagcgttcag gtctgtgcca agggcattca
    18481 cactggtcag attcaaactc acatattttt tatccgtact gtcgcgtttt acccagtcaa
    18541 ttacatcgcg tccaagccgg taataaactg atgccgaagc tgtcagattg cctgaattaa
    18601 ttttcgagcc aagttcgtag gtctgtgatt tttcgggtcg aagatttgga tttgcaatct
    18661 gttgcgcccc tttgtaatac aggtcggtaa aggtgggtag acgcacagcg gaatttgcgg
    18721 aggcatatat gcgaatatct ttcgataaag aatagtggat atcggctcct ccgtttacat
    18781 gtgtatcgaa ctgtttcgca tggctcagag ctgctcctcc cgaaaaagtc cacttatcca
    18841 ttctgatcaa atgatccaga aaaatattgg ttatcagtcg gttatcttca aacgtgtatt
    18901 ttttattttt ttcaaacgga acgggctttg gtgtttccat ttttacaccc agcacattac
    18961 tgaaaatatg ttcgttgcga atatccacac ccagtgtggt tttcccgata gcccaaacgg
    19021 atgaaccggt aatttttccg ccggtaatgt cggtaaggtg gaagttgtgc ccggtgtacc
    19081 acgtgaattt ttcggtacct tccatgtcac gaaataactc aaaacggtcg tgatgctccc
    19141 gccaatatcc ttgtgcactg agataaaccg ccccgatttg gcgattccaa ctaatcgaag
    19201 ccagaaaagt tttggtgtgt tcaaactggt ttgggtaaac cagggaataa aatccgtttg
    19261 acccgaatga tttaagttgt accgcagttt gcacttcgat aatacctgcg tttccggcgt
    19321 ttattcttga ttgaagataa atgttgctaa tatcaaaatc ggtgttgtca atgtacccgt
    19381 ccgatttttt ggtgacggcc gatccgaaaa cactccattt tttatctccg taattaccgg
    19441 aaatactttg tgacagataa ccgtaacttc ccacagcatg ttggcttgta atgctgtatt
    19501 cagaagtttt ctcagtcaga tttctgcgtt tttctttatc aacagaatca ttaatccatg
    19561 tttccggggc attttttccc ggttcggtta ccgaatgtct ttcaccggtt acaatattga
    19621 ttaccccgct gaaagcattg ggtccgagca cccgggctgc cgatccctga agaatttcaa
    19681 tccggctaac atccgaaagt tccagcggta tgtccaggtt gtaatgaccg gtttgagggt
    19741 ccgtgatatt tattccgttg agcaggatca gtatctgatc aaaggagcca ccccgtatac
    19801 taatatcggc ttgtacgccg ttaacaccgc gctgacgaac atcgataccg gccacattat
    19861 ccagcagctc atcaatgctt tttacgggca ttttttgaat ttcctgttgg tcaataacag
    19921 tgactacacg actaattccc gtgtaaagat ttgttttttc agcgctcact tcaatttcat
    19981 tgaggtgaac gctttgttct tttgattgtg aaaatacagt gaatgatgaa agtaaaatta
    20041 atagaaaggc aaatgatttt ttcataatgg ttaaaattaa aacagctgaa tatcaggatt
    20101 aacctgaata ctcagctgtt taaaatgtga ataatatttt tgagaacgac ctgctaaatg
    20161 gtcgatagtt gaatttgcaa ttattagaat ttcggacaat tcggaaagta atggattaca
    20221 tatttctttc tcttcctctt cggtgctaac agtttcggaa tttactgtaa aattcgcaaa
    20281 agcaggttta ttggatttca aaagcgcttt gtcactgatg ttaaatgcaa tgtgaccaat
    20341 cgatatttca cggtgaagtc cggagaagac agcaaaattc ttccggctcc agcgacggaa
    20401 acggatagaa ctgtatttat tttttgctgc tgatttgttc aaagccagag taatgttttt
    20461 tgtgcgggat cagtattttg acgggtcaac aaagttataa aaattagctt tcactgtcca
    20521 tacatctgaa aatggcataa ggatactttt tcctccgatt atgtacaagg tgttgttggt
    20581 cttgttgtga atcagggatg agttggtccg gtatttgaaa cttgcgggaa gtttgttctg
    20641 ggttgtatca ggttttgccc aggtgaggcc ttcatcaatt gattggcgca tctgaaaaga
    20701 ggtgtctctc aattgatcgt aagtatcggc gccgcctatc aaaatcagtt tgcttccgta
    20761 gtaggctatg gcagcccctg atactgcatc aaacttgcta ctgctgttgt tgaggttgac
    20821 ccagtgaagc gaatcggtag tcggatcatt ttcggccgac caacgggtat ttaatttttt
    20881 accatcagca tcttttccgc ccacaataat cactttttcg cgaccaagac gtggtgtgaa
    20941 ggttgttgct gcaaagtcgg aaaccgggaa gcgggtggag agagcatttt gcacagacca
    21001 cgtgatgcca tcctgtgaac tggcaatatt taaccgtttg gtggttttat tttgtgcgac
    21061 agaccagtac tggtttttga aattgaaaag cagcaccaga aaatcgtagt tattatcacc
    21121 gatgagcagg tgtttttccc aggtcagtgc gtttttggaa gtgtaaagtt cattgccgtt
    21181 gtgcaacaat agaatctgat tattgtacac gatcatattc cggaacgtgg caccggcagg
    21241 cagaccggca ggcgtacttt cgggcgacca tgtgctggca tccgctgatg aatatagccg
    21301 ggttgtggag ccgttgcccg tgaagaaaaa gtatttattg ttgagcaata ccgctttctg
    21361 gttgtcggtg gcatcttcgg taatattgct taccggattt ttccagacat gcaggtaagt
    21421 ctcaacttta tgtacattta caattatggt gtattccttt tttgattttt tatcggaagc
    21481 gtaatttgta attttaaccg gtttggaata attgtatgac ttactcgtaa tatatgatgt
    21541 ctcgggaaag gtgtcattga tcaggtagcc atcagtactt gcaaatgaaa atacagcata
    21601 agcgctgtcc acacgcgttt tataaggaag ggaatccgga ttataaatca gccctttttc
    21661 atggtcaact ataaaaaccc ctttcgaaag attgggggaa ctgtcgttgc tctgaaaata
    21721 catggctttg acagtggcat catccgatac cgaaaccggt gtcgcagtag tattgcagga
    21781 aataaagaag gtggtcaacg aaagtaaaaa caaaagacaa atatgctgaa agactttcat
    21841 gttgtaattt taagaaatac cggaaattag tgtgaaatat ttatattgca aagttaaaaa
    21901 caatttgcag taattttaac ctgaggtcga agttttatta ttttttatag ttataatcca
    21961 tctgtaaacc accgcatcag cattataagg tctcctgtta attgagttct accagtacgg
    22021 gacagtggtc tgaatgtacg gcatcggcca aaattgcagc cgatttcagg cgtggtttca
    22081 gcgggtggct cacccagtga tagtcaatgc gccagcccat gttccgcgag cgggcagcgg
    22141 ttcggaagct ccaccaactg tatttatcgg cacttttgtc aaattcacga aaagaatcaa
    22201 ccattccggc agattcgaat tcgtccagcc aggctctttc ttcgggtaaa aatccggaaa
    22261 cacccgtttg acgctcggga tgattgatgt cgatgggctt gtggcaaata ttataatcac
    22321 cgcaaactac tatattggag cgtgttttcc ttaaatcgga aataaattcg tggaagtcgg
    22381 ccagaaatcc cattttgaat tgctgccgtt cacctcccga tgaacccgac ggaatataga
    22441 cacacaccac cgtcaggtca tcaaaatcgg cccggagtac gcgtccttcc gaatcatatt
    22501 tgtgattatc catcccaacg acaacattgt ctggcgaaat ttttgtaaat atcgccactc
    22561 cgctgtatcc ttttttttgt gcggaatgga tgtaacaggt gtaacccata cgattgaaaa
    22621 tctccgtgtc aatctgatcg ggttgagctt tggtttcctg caggcatagc acatcgggac
    22681 tggcttcctg gagccattct tcaaaacctt tggacaaggc agcccgaatg ccgttgacgt
    22741 tgtatgaaat aattctcata aataatccgg taaaatagga atataaatcg tggatgtttt
    22801 cagctaattt gttaatcttt caattttcaa atcctcaaat tttcaaattt tcaaatcttc
    22861 ttactgatta atcattttaa cttgcttgta taactccgaa gcgaatacga aatcgttcaa
    22921 atcttcattt ttagctccgt atatgtcttt attggttccc gaccaggcca tgagcccatt
    22981 attgataaag ataatatttt caccaatttc gatcaccgaa ttcatatcgt gcgaattaac
    23041 aatggttgtg atattaaatt cgtgcgtgat ttcgaaaatt aatttgtcaa tgatgatgga
    23101 tgttttggga tcgagacccg aatttggttc atcacagaag agatattttg gattaagcga
    23161 gatggctctg gcaatagcca cccgcttctg catcccgccg ctaatttccg aaggaaaaag
    23221 atggtacgaa tgttcgggca gattgaccct gtccagacag aatttagccc gaagttccat
    23281 ctctttttta gtggatgaag aaaacatttc caggggatac attacatttt ccatcaccgt
    23341 gagggagtcg aacagggctg aaccctgaaa cagcataccc acctcccgtc tgagcagacg
    23401 gatatctctc atcctcatgt cgggcatatt gcgcccatcg tattcgatgc gcccggaatc
    23461 gatccgatgc aaaccgataa tgcttttcat cagtacggtt ttacccgagc cgctctgtcc
    23521 gattatcata ttgcatttgc cggtttcaaa cacagctgag atgtctttca atacctcttc
    23581 tccgtcaaat gacttaaata aattggttac tttgatcatt tttcgggttt aatttagcat
    23641 taagttcgtt atcagcagat tgaaaaataa aatcagtatg ctgctgttga ccactgcatt
    23701 ggtactggct ttacccacat tgagtgcacc tccgtaagcg taatagccgt aatacgaagc
    23761 taccgacgaa ataagaaatg caaaaaccaa cgatttgaca acggaataaa aaacatagta
    23821 gggaatgaaa gcatactgaa tgcccagcag ataatcggca gtggttataa tatcgccgaa
    23881 tacaccaacc agataaccac caaaaagacc aattgccata ctgaaaatga ccaaaaaggg
    23941 catcatcaca acaaaggcag tgattttggg taaaatcagg tagtttgccg aatttacacc
    24001 catgatttct atggcgtcaa tctgttcggt tattcgcatg gtgccgattt ccgaagcaat
    24061 attagaaccc acttttccgg ccaaaataag acacatgatg gtggacgaaa attccagtaa
    24121 cagcgtatcg cgcgtcacca aaccggtgct gtaggttggt aacagtggat tttcggtgtt
    24181 caacttggtt tgaatggtca taatggcacc aataaaaacc gaaatgataa tcacaatggg
    24241 cagtgaagcc aatccgagtt tctccagttc tttgggaaac tgccgccaga acatacgtcc
    24301 gcgatcgggc actgcaaata cacggtacat gagcagggcg tattgacctg tatctttaat
    24361 aatactcata tcaaaacatt tttatgttta atttttaacg tgcaaagata caaattattt
    24421 atcctgcttg tgactactgc gatacttttg aaaatgctga acccttcaat ttttataaga
    24481 tatgaagact tttattgttg agttttctaa aaatacatat ttacaaacaa agattaacgg
    24541 ttattcgata attcttacat accgatgtta attgcctgat atataagacg tcagtgctta
    24601 aattcactgc aaataattgt caaaatatgc acataataca gaaaaatgtg cattttatat
    24661 tgcttgtatt gcacaaattg tgatgaaatg tgtaattttg cacttcctga aactgaaatt
    24721 tatacatctg cgattaaatt ttgtcaggtg aaaagctata cattcaaata aaaaataatt
    24781 ttatgattaa aaacttaact attacagcac ttttagtatt ggcaacaagt tcactgtttg
    24841 ccggcggtct cctcaccaac accaatcaga gtgtccattt tctgcgaaat cctgcacgcg
    24901 atgcttctac cgaaattgat gcagtttaca ccaatcctgc aggtgtggta tttctatccg
    24961 atggtttcca tatttcactc aataatcaaa gtgcatttca aacacgtacc atcacttcca
    25021 cttttgcacc gtttgccggt tatggcggaa gtgctaccaa agaatatggt gcaaaaacta
    25081 attctccggt tgttccaagc atacaggctg cctacaaaaa agaccgtttt gccatttctg
    25141 ccggatttgg cgtagtgggc ggtggcggcg tggctgcatt caataagggt ttgccctcgc
    25201 tggaagcacc cgtttcgatg attccactgt cgctgactac gaataaaata cccacttcat
    25261 cttactcagt ggatcagtat atggaaggaa aacaatacac ctttggcgta cagttgaatg
    25321 gttcttataa aattaccgaa catttatcgg ccggggttgg ggtgcgtgta aacctcgtga
    25381 gcaatgccta cgccggacat ttgaccaata ttatgattgc tccgaatcaa ccggcttttg
    25441 gagcttctta caatggatca acattggttt cggcaccaaa attctttacc gatgctgcaa
    25501 ctaccattcg tggttgggct acaggtgcta catcgtatgt agccggtttg caaccaattg
    25561 tatccggagg tggtggcaca gtattgctgt ctaacggaac tgcagcggga ttgagtgctg
    25621 cccaaatagc tcaaatacag ggtctgcttg gcgctgccgg tctgacacct gctcagatag
    25681 gtgccataaa tattcaaaca gcacagggca cgttggctgc agcagctcct gtttttaccg
    25741 gaaaagccga tgcaatgaca gcaagtgcaa gcggtacagc cgacaaacag cttgatacca
    25801 aacaatcggg catgggattt acaccgatat tcagtttgaa ttacaatacc gaccgggtga
    25861 atgtggccgt gaaatacgaa atgaaaagta caattaccct gacaaacaaa acgacggtgg
    25921 ataataccgg aatgtatgcc gacggtgcta aaacacccaa tgacatacct gcttacctgg
    25981 ctgcgggagc cgacttcaaa cttaccgaca actggaaagt gtcgggtggt tatcatcatt
    26041 tcttcgactc cgatgccaaa atggcaaatg acaaacaaca atacatcaac ggcggtatca
    26101 acgagtatct gttaggcaca gaatataaat ttggaaaaag atttttgtta agcgccggtg
    26161 gtcagctgac ccgtaccgct gttaccgacg attatcagtc tgatttgagt ttcagtcttc
    26221 actccgtgtc ggttggtttt ggcggcgctg tggatctgac cgaaaatcta cgcttgaacc
    26281 tggcctgttt gttgaccaat tattccgact ggacaaaaac gtcgaccaac tataataaaa
    26341 ccaatttaac aggaaccgat gtgtatgccc gtacaggtaa aacatttggt atcggcatcg
    26401 attaccggtt ttaattaatt cgtacatacc tttctttgat aaccaaactc taatacattg
    26461 aaccaaaaaa gcgaaactga at
//
LOCUS       DAOPZK010000011        25774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1888_length_25774_cov_23.284226, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000011 DAOPZK010000000
VERSION     DAOPZK010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25774
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1888_length_25774_cov_23.284226"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PKH68_01300"
     CDS             <1..983
                     /locus_tag="PKH68_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNX11600.1"
                     /translation="RLISRKPNSYMNAFVFWELMNAYVDGWGNSYAVVERDNYGDPVA
                     LWPILPNNILPILYKGELYYRVFTGDYAGDYSSSEILHFKLFTKDGIKGIDPISNMSQ
                     AIGLGLAAERFNAEFFDKGGHIKGVYETEQSLGDEEFNRVTAHMNAAKNFETPLLEYG
                     IKYKQITISPEAAQMIQQRTFTIQDIARIFNLPPHMLAEQSRSTFSNIEHQDIQFVKY
                     SIRPSVKRYESEIEDKLFFDGEKDFLDTKFNLEGLLRGDTQTRSAFYHNAILDGWMNR
                     NEVRNLENLDNGPVELDEYLYPSNMNLAELLLGQQQQKNNSQPQNIDNGN"
     gene            973..1770
                     /locus_tag="PKH68_01305"
     CDS             973..1770
                     /locus_tag="PKH68_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HNX11601.1"
                     /translation="METKKYTFGTVRAFDPAMVEKTRTIEFVISTNTRDRHGTILPVD
                     KWQLDNFNANGIVGYQHNVYGGDLCNAPDPDDVIGSGRAWIEGDQLIGSVTFEPAEIN
                     PKAEKIFRKVLSGTLKATSVGFNPIGDGAWGIGEESERGTNPTYYFAGQELLEFSIVN
                     IPSNPDALRRSLRNQASDAIAYIYRQLNGDYRFSQIEEMTVRQVIDLLDMTPEQRALS
                     VKVEVEIEESDPKPDELPVIPEPDPQPSTSDEDLVVATRAKLALMAQ"
     gene            1792..3057
                     /locus_tag="PKH68_01310"
     CDS             1792..3057
                     /locus_tag="PKH68_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNX11602.1"
                     /translation="MAKKSFEIRKELGEKTNRFEELRVKDQRSAEEQTEFDNLRGTIR
                     TLTNDLDDAELTEAGKKRLASQGLSNQEREQAGNYSFRKAISDFVNRNGNLEGFEAEM
                     HQEAVREAKANGKTLKGIGVPMMVLENARAATGQNKGTANDGGNLIQDEPLVYIEALR
                     NRLVLVGLGARFITGLVGNLPLINGGTFSAEWYGESDPVTPGKLSLPKKTMSPKRLSV
                     AGAYSLELINQNSISVEMLLRDELMKAHIAGIEGAAINSATNGPVGVLNTSGIGAVAG
                     GDNGLAAAWSHIVDLESKVAIANADLGALAYLTNAKVRGKLKQTLQAAGVPGFIWDKE
                     GMNGYKAAVTNAVPSNLTKGTSEGVCSAIVFGNWNDMIIGQWGGYDMVVDPYSKKLNA
                     EVEVVLHSFHDVALANPASFAAMKDVLTA"
     gene            3163..3462
                     /locus_tag="PKH68_01315"
     CDS             3163..3462
                     /locus_tag="PKH68_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX11603.1"
                     /translation="MKIKFKKSAPGFAYFAGDVADINPEAAAKLVDDGFAIIVPETEG
                     DDNELPEDLPGRLILFKEGIKTLDDVKKITDSLTEIKGIGKKLASEIVAYIGNLQ"
     gene            3492..4037
                     /locus_tag="PKH68_01320"
     CDS             3492..4037
                     /locus_tag="PKH68_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HNX11604.1"
                     /translation="MYILIIPATTLPVSVEELKQDIGIFHTEKDSVLSIYLQSAVDYA
                     ERFTGRQLMPAVWELQIDTFYEYLKISKAPFIDLVSIKYYDANNVLQTLASGTDFDVC
                     SSDPALIHFKTDFTLYHRPDALQIRFNAGYASAEVVPPMIKAAIILMAGKMYDSPIDS
                     VENLPKASTNLLRQYRLWQQN"
     gene            4022..4354
                     /locus_tag="PKH68_01325"
     CDS             4022..4354
                     /locus_tag="PKH68_01325"
                     /inference="COORDINATES: protein motif:HMM:NF017343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HNX11605.1"
                     /translation="MATELVSVKDFNELIMFRQPSRDKSSKGQVKETWTDYRKALVKV
                     IESGNDEQKEGDRMNLPGVLTISGHYDSGVNTTFRVLWNSVEYAITSITPVQNRRFMN
                     VRAERVFE"
     gene            4354..4860
                     /locus_tag="PKH68_01330"
     CDS             4354..4860
                     /locus_tag="PKH68_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11606.1"
                     /translation="MAKQPVTIEFTGTENIQEIFNKLPEQYAKKPVQAAFRKAAKPFI
                     AAVRSNLPSRLSSLQKIINVVNNKSAGITAGVLSKKAMVTLKDKREYDAFFPVYWSNY
                     GTYSRRDASHKFTQKRKAKTANRKGGIVPLRFVEKSWDQTKAQVEEIAQRELITQTDK
                     FLKKHAIN"
     gene            4864..5160
                     /locus_tag="PKH68_01335"
     CDS             4864..5160
                     /locus_tag="PKH68_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11607.1"
                     /translation="MSPFEIVSTIVAILGLAVLILKVWIKSQTDIAAIKVEINATNNR
                     QKNMENDLKQHKLDDNFRYEQMRKENREDHGKLFEKIDKIIEKQDDNRRAHSNN"
     gene            5129..5491
                     /locus_tag="PKH68_01340"
     CDS             5129..5491
                     /locus_tag="PKH68_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11608.1"
                     /translation="MIIDALIATINPIVDCYTAIGDLKAELPFSVIQVAPEPIRIKDG
                     IVGYTQKVSVALVDNDVDRIEENTVAVRAAIEALTGLVEECQINSVMFESENGVIFDP
                     ETQTYQNSFEFTVDTNTR"
     gene            5521..5940
                     /locus_tag="PKH68_01345"
     CDS             5521..5940
                     /locus_tag="PKH68_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11609.1"
                     /translation="METRTMGYNITLKLNSKKVMGTTDNDFNIDAETKESLIKDDLGK
                     SQIDYSGYKATFGINGLSMTTDETEPTKLTQNDLISAIVNKTKITFVYSRVGSTATIE
                     GNLVITKYSESSSSSDHGKWTVSAQVDGDLTFGTLHA"
     gene            5994..6356
                     /locus_tag="PKH68_01350"
     CDS             5994..6356
                     /locus_tag="PKH68_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11610.1"
                     /translation="MKNDHVEINGKKYRVEFNWNAITDFLESEGLQLSDVDDLRELKP
                     RQVTGLVYAGVCEGCRLDGKEFTFSKLEFGAMVKPPQIGELLLIYQRHTSVKKSEVAE
                     SVEAQKKRFQNPFRSKKS"
     gene            6440..6667
                     /locus_tag="PKH68_01355"
     CDS             6440..6667
                     /locus_tag="PKH68_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11611.1"
                     /translation="MNAKVNDEFKRDAELTRLQTATLINIQLKKEDQIKPRDLWRFEW
                     DDQETENESGLTEEQIIEHNNRLSAVFNNKD"
     gene            6673..8601
                     /locus_tag="PKH68_01360"
     CDS             6673..8601
                     /locus_tag="PKH68_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11612.1"
                     /translation="MAETVTNLKVRFGADTKNFKADLESGKAAVDKFTGEAKDAFSEF
                     ASVFGINMNEVSAKFSSVQKSLSLISGGLKGTTDGLKGTTEAVRIFGVSWKVALGPIA
                     LVIVVIGSLVTALTRFSDNAEKVKVVMAGIGGGIDVLLDRFAKFGSAIIDVFSGDFVG
                     AAQKFKETFTGIGAELENDIRLTAMLKRATLDLERESKVFDAQREAGMTKILQLRERE
                     KDQTLSATERLKANAEANKIEQTLAERSLELADQETAATLDNIDAQTKKLRLSQEAIV
                     LIEKIKNGTIGAMEAQKAADELDLGDKDGKESLFALVDAIVKREKEQQEILQTRLRLQ
                     KSENTLKNEILKQEIDGLNSLASLKQQQSQNDKKTDLERTKLILEASELQKKALRIDF
                     DANNISKEKYLAELDKLAIETEEKIKAIREKLNPKMETLSAIKLTVDDKTGKIAQTEI
                     PAPKIGELETSKLEAKAAKIQSIYGNLKSTVVDFSSVFQESMTGLAVGFAESLGTMLT
                     GATGFGDLAAMAGAALGDMAIQVGKIAIQTGIASLAIWESLKVPGGGALAIAAGIALV
                     ALGTAAKAALSNVASGKGNYSLGSSTSSYGSGSYGSSSSPVRSAPQTIYITGEFRQRG
                     TDLVAVIDKNTQRRVIGT"
     gene            8698..10956
                     /locus_tag="PKH68_01365"
     CDS             8698..10956
                     /locus_tag="PKH68_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11613.1"
                     /translation="MAYGLKYILRYETQRFGTDIRVLIYEKDYTGPSQPKRIGADHIR
                     LSRDKDGQICGTTLDFSIQADTDMEFANFFTLDNKAHKVELEFNWGVVWTGFVVSDQY
                     SEPFVAPPYDVQVMATDGLGLLKNEKFELTGMVSRFEAIRYCIDKIGIALPYAININI
                     WEQNMNISGHNMLDQLYFHAEILRDKTCYEAIQALLPENVFINQANGYWMIERPTDRT
                     LPRYIFNADGTPSNEEYNWHTFLRELNTVQYSEIHRGSLYPIGSLTLEMQSAWRKFTI
                     KQIFGKRESFFRNHDFKAGTTYWENVGLGANFYAKNVNEENLGVLAGYQNSYVNHISQ
                     SIDVISTSAITLEINYALVGVSPIGGNINFSKFNPNVKFQLKLAGTSGQNYYLDQQGW
                     NTSAKEILIEKVPYSFTFEDINFQSTKINAAKLPESGVLTITIYQVIPNSNVKAYENW
                     ADVYKGLFVKKVIFYNNDITQFNDSEETTVILNENATESEKTIELKPTDIPNYENNNL
                     YFDNGNYVKVNGKYIPTKSWGSQQKTYLEYMSAYMAYLYQSSRSVLKGEISGWLELNS
                     YVVHYLTNKIYLIDKGTWNVLDHTFTLDLIELPAGEFIYDNYAQNLPEPFVPHVDISD
                     VNDFIVPIDGSFEVPLTGTHTPLIVARAQTIDPLKLDFDSGTPQFSIKDAVVETNVGD
                     NANAVKIGAGEIISHHYAALDRTSIRFIKEANEQYDPTRAWDIAETNIELPDNDGYFL
                     YVKVPLAEQLPE"
     gene            10953..12902
                     /locus_tag="PKH68_01370"
     CDS             10953..12902
                     /locus_tag="PKH68_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11614.1"
                     /translation="MIRTSEILTSKTHIEPLRDEGFVIYPIAYIGPVINGKREAYSKG
                     GNIKGTTTIDEKVRLIDPFTFTPMGQAGYLSGQYFDQYIDNGTGRAIIFPIKQEKIIT
                     QIAHGFEFGNAIKRDGAGAWIRAKANNRANAGTVGIVSNVIDADNFEYLPGGLLPGDF
                     VDGACYFLSPYYDGQIFVQSDPEVWTVGQIREFIGTGTAQGLEIEIDLGDEIIDLQDL
                     KGPQGAQGSQGAQGSQGAQGSQGAQGNIGSQGSQGTAGIGLNLLGEKNTPADLPSSGN
                     SAGDCWNVASDGHLYAWNNANWVDVGQIRGPQGPQGSAGSQGNQGAQGSGGAQGNQGV
                     QGAGTQGPQGNIGPQGVQGSDGQQGYQGTQGIRGYQGNQGNQGIQGVQGNQGYQGSQG
                     ATSTVQGPQGNQGSQGSQGVVGAGYTASSSSSVSAGTGFKYFTVQSGLAYSAGARVRA
                     YTGGSVIILEGICTSYTGNQLTIFVDRSVGSGSASSWTINLAGEVGTQGAQGYQGVQG
                     AGSQGPQGYQGVQGTQGPQGVRGSIGMGLQGPQGYQGASLWTDAGAYIYPTQYSDAVL
                     IGAQTRNYGTSNILEVIGSSAFSGIVNLMSALFLSGNYIAFADAHSLPGGLENGGILY
                     TDPSDGLLYYKRSNAKGGGTYLLSK"
     gene            12912..14099
                     /locus_tag="PKH68_01375"
     CDS             12912..14099
                     /locus_tag="PKH68_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX11615.1"
                     /translation="MKISLFTPTHDSKYLSEVYESIKDQDFFEWLVIYNNGAKEIDFN
                     DIRVKTIRLTDAPGWVGPLKAFACENCKGDILVEMDHDDWLHGDAIDELKNAFKDPEI
                     GFVYSNTIHDKEHHYSEEYGWKYRPMDGQWEEHISFAPTPDSISKIWFAPNHLRAFRK
                     SVYEQIGGYNKEMRVLDDLDLMCRMYAVTKFKHIDKGLYYYREHSENTYLKYNAEIQN
                     NVFRIHDQYIESLCERYSDLNGLKKVELGGRIAAKPGYETVDLKDADVICDLNEPWPY
                     ADSSVGVIRAFDVFEHLSDPLHTMKEVQRILAPGGWLICQVPSTDGRGAFQDPTHKSY
                     WNENSFFYYTKSFFAKYIDTPVRFQASRLYTTEKNQDQVCWVVAHLVNLKDGFRPAGL
                     VEI"
     gene            14111..14911
                     /locus_tag="PKH68_01380"
     CDS             14111..14911
                     /locus_tag="PKH68_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11616.1"
                     /translation="MAGGRAGTNIVVLNGPQGAQGIQGLQGPQGNIGAQGSQGSQGIQ
                     GQAGNQGAQGSGGAQGNQGNQGPQGAQGNQGQVGNQGAQGSDGAQGNQGNQGPQGSQG
                     NQGAQGAASTVQGPQGYQGAASTVQGPQGNQGAASTVQGPQGTQGYQGAAATLTDNSV
                     TYAKIAAALKQKNAVTASVDLSANGIGEITLSTNTAFSFSNFELNKTYLLKITANGYT
                     PSWSAAAKHVPVEGNATFGTSGVFYVSLTCIDATAGSEKLLTLIMKGV"
     gene            14913..15827
                     /locus_tag="PKH68_01385"
     CDS             14913..15827
                     /locus_tag="PKH68_01385"
                     /inference="COORDINATES: protein motif:HMM:NF027230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11617.1"
                     /translation="MLTPGIGITCKPSALSVEGKYQVACDISTGIYCSSDYGQTFSLK
                     LSIANKNWSGVGISATGQYQVAVTNNTMTYLYLSTDYGNTWNTTTLLIQSLSVCINAT
                     GQYITTGISGYIKRSINYGSSFSSVTQAGISAGFSCSSSGQYQVVVMQPGYIFISTNY
                     GLTWSQKGNSRSYTGVSMNSNGTYIIAAVSSNYLQFSSDSGQNWSGKVGSKSWQSVSI
                     SSSGQYVFAVVYSGSIWKSEDYGQTWSEIVITKKWMSVTVSATGKYVTAVADSSQIYA
                     SSNYGQTFSQINSSRAWSAVAINRFSDV"
     gene            15840..16313
                     /locus_tag="PKH68_01390"
     CDS             15840..16313
                     /locus_tag="PKH68_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11618.1"
                     /translation="MKHLIKNGQIIQSGLPGTFTRENGEGFWGGYEERTDIHFIDGWR
                     DEIIPEYNPILEKLSEPFYDTDNDVVTYEVIERNDLPPLETAKADKIKAIKLYARDLL
                     FATDFYIVRRAETGKSVPQEILNARQAIRVWNDAQEKAVLALTTFEEVASFEIKY"
     gene            16381..16785
                     /locus_tag="PKH68_01395"
     CDS             16381..16785
                     /locus_tag="PKH68_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11619.1"
                     /translation="MKTLEKIVQFFYGLKTLTLEFMNKLKTVREKNILTIIAIISIAL
                     FVIFYIYGKKYELQVVPIGMLQKVIFAMIAGTTGPLTALIFLKYGRPDSQDALSSDTE
                     GGINNLTDWERVKLSMFWIAAFIIPFSIALLA"
     gene            16788..17390
                     /locus_tag="PKH68_01400"
     CDS             16788..17390
                     /locus_tag="PKH68_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11620.1"
                     /translation="MKPFTLILLILIASQAFAQTLEMPTSPLRDKLVAIADSYMGIRE
                     ATGRNDGPKVRKILRNVGLPEGNAWCAALQAQVHDDCNLPNPHSAYCPDWFKSNVVFE
                     KDEMSIDKFQAKRGQVFGLWIEYKHRVGHMGMITGESRFSYYTDEGNINSKGSDEGEG
                     SDKYIRSKRSIHVIADYAMSREEKNLYLGKHRKIILKDAD"
     gene            17565..17909
                     /locus_tag="PKH68_01405"
     CDS             17565..17909
                     /locus_tag="PKH68_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11621.1"
                     /translation="MNITNRIENILIAAAAAIIAIALTVAIMEMTSIAALKKENSRLI
                     TQADKQNAVIFELAKIEKYKIENRFDQVKAKDGQVVMSLDNKLSALSLDSIKPMTKPK
                     VKKTFWQKLKFW"
     gene            18164..19432
                     /locus_tag="PKH68_01410"
     CDS             18164..19432
                     /locus_tag="PKH68_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNX11622.1"
                     /translation="MATFKAVILNGKAFEKSDGTTNIKIRITHKGKQDYVSTKYFVLV
                     STEKKSYFDSTTGKAKGTSDAKFINDQISVLIASYIKAESALGDSSDQMTVKQIKNHL
                     TQRKSDKYIDIIEFTENFISRLEKLKKDNTAQWHRSMLSALKNFVKNTKLNVLDVDSA
                     FLNRFEQYMLKGDKTTNPVREPMTEGGINNYMRSFRSIFNKMKDKYNDEETGIIRIAK
                     NPWKVYKVKTPKRRQQGKNMTVDEMKKLVAYSSTNKGKQFARDMFLLHFCAIGINTKD
                     LYFMPKQTGPRLEFDRFKTQRAYSIKIEPEMAEILTRYESDSRLVNIHIKYKDYRIYT
                     RQIDVFLKKVAADLEIEKPISSNWARHTWATFARINCKIPKDDVALCMGHEDADNKVT
                     DDYITYDYSIIDRSNRKVLNLIFKKSDIRK"
     gene            complement(19564..20019)
                     /locus_tag="PKH68_01415"
     CDS             complement(19564..20019)
                     /locus_tag="PKH68_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11623.1"
                     /translation="MKTLFTTLILLFFASAVFAQDDAYYSPEKKIANKIIIETRKPKA
                     ENLKLCVDILTENEIVCEQNKETFIVKTDIQNTKKRNYPYYLNFFCKDSVIVVSGKFK
                     LGVSLEIYGVRSEDNFEQIQNNGMKGSAYRITFDEMKTVAEKFGKPINF"
     gene            complement(20026..20565)
                     /locus_tag="PKH68_01420"
     CDS             complement(20026..20565)
                     /locus_tag="PKH68_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11624.1"
                     /translation="MKNILIVLLCFILFSCSPSKVEKLISDYEQTIGNTKTDLSLKIH
                     ELKEVQKITGKDSSNFFKAKVDSTIKILSGDKIKTFKSINGTLDTLVNSYTHILEMDY
                     SDFYAEKLKYWSEIRTKLRINNLKAFKYEIVNDSILAIKYNCTYTIKNPILNNAKQTI
                     SKSYYVSKNLSKVLKTSEN"
     gene            complement(20647..21015)
                     /locus_tag="PKH68_01425"
     CDS             complement(20647..21015)
                     /locus_tag="PKH68_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11625.1"
                     /translation="MNLTEKQIIDLKIVLSGKANDLLANEIGIRKEYFSKLEKYPGKT
                     EKIKKYFNENYPEFDLAIKKLTEFENVIQEPEGIYEKRKCLNCNDLEKQVQYWKGKYE
                     GLKEGLVLKNEESYKQTGSE"
     gene            21163..21555
                     /locus_tag="PKH68_01430"
     CDS             21163..21555
                     /locus_tag="PKH68_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNX11626.1"
                     /translation="MKRTLTPRETEIVELVAFGLSQKEIGDKLEISPNTVDVTVKNIK
                     TKLDLQKSTELAAWYFINTYHITLNLSPIKRAMISLSFLALMVVSLFEGFNPERSFRT
                     IRASRAQVSLTLRSGRRSNESDFYLLTA"
     gene            21558..21836
                     /locus_tag="PKH68_01435"
     CDS             21558..21836
                     /locus_tag="PKH68_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11627.1"
                     /translation="MSEIRLTRFELGQIISTAVEIGVSRATEKKAFLSKRAADIKYGR
                     RAVDRWIQDGKLQPVMQKRRVKLDADRLNELSKVDELYGKFIGEPEND"
     gene            21841..22080
                     /locus_tag="PKH68_01440"
     CDS             21841..22080
                     /locus_tag="PKH68_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11628.1"
                     /translation="MSAKKSPISVREAAEQVFAEMPETFHAIFFCARVKVRTGRAALM
                     DGTILRRLREARADSQQFQYRCIDADKSIYQKIAI"
     gene            22485..22556
                     /locus_tag="PKH68_01445"
     tRNA            22485..22556
                     /locus_tag="PKH68_01445"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            22852..22942
                     /locus_tag="PKH68_01450"
     tRNA            22852..22942
                     /locus_tag="PKH68_01450"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            23324..23527
                     /locus_tag="PKH68_01455"
     CDS             23324..23527
                     /locus_tag="PKH68_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11629.1"
                     /translation="METKIIICVAIVFALFVVFMFVTASLEAKNNRLSAENDDLKGEV
                     EIRDIQIEILKNIISDSEKETRF"
     gene            23529..23759
                     /locus_tag="PKH68_01460"
     CDS             23529..23759
                     /locus_tag="PKH68_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11630.1"
                     /translation="MKLKTAEERAIPFLKNKVKLDDYNKNGMFKDGFFNPICPPDYNF
                     ITGKQEPLITSQRGMREVSLARRVNGTPIFRN"
     gene            23761..24630
                     /locus_tag="PKH68_01465"
     CDS             23761..24630
                     /locus_tag="PKH68_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4373 domain-containing protein"
                     /protein_id="HNX11631.1"
                     /translation="MARPQKTNADYFSHDTGMRNDLKIKAIRRKFGLEGYAIWCMMLE
                     VLTDSDNLELEYNETELELISADFDIDAEKLKAIIDYCVSPLRILAVENGFLFSETLK
                     KRLDSLFQKRKRQQKIVIDVDNSKKYEFTNVLAVDNTHSKVKESKVNKIKVNESKENT
                     NSEENQLVFFENTDIHFSILEFFGFTEMRNPDKLQQISIFLNILFTDGRINQFTDQFE
                     AYRLYKEKSSTAKHSFPRFLGTIETRYLDGGWNQENWTDRLNAIAKRESSDSPFNSEK
                     TQSLRERIVNGKD"
     gene            24617..25222
                     /locus_tag="PKH68_01470"
     CDS             24617..25222
                     /locus_tag="PKH68_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11632.1"
                     /translation="MEKTDFSRTEIKDISRTDIAGLVFDIVEDLHKMCSQKIDDDNVQ
                     FTVKKFVELLNGPYRSWCWGEVRSVMNLGVTGSYGGKSEKINFQTLATWMKRSQAGRS
                     SNYAERSIYEAHQIAGETVGNFADISDRWKEFREWMQENHLWFVDNISEENCNLYHSA
                     KRTRSLENFKDMLAIEPDPGYMERVKKTTLEQFRIEHEILD"
     gene            25206..25559
                     /locus_tag="PKH68_01475"
     CDS             25206..25559
                     /locus_tag="PKH68_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11633.1"
                     /translation="MKYLIDFDIRLSEDKKTLELLRNNQVFEKIEGEQANEIYKKLVK
                     IDDLKLLGFSYCYLEFDGKLDEWYELRLVEQNALTEGVSLMYHPKTDLFEVPENGLVF
                     SKYEKVEKYIKAFSL"
BASE COUNT         8332 a         5012 c         5508 g         6922 t
ORIGIN      
        1 agcgacttat tagtcgaaaa ccgaacagct acatgaatgc ttttgtgttt tgggaactga
       61 tgaacgctta tgtcgatgga tggggaaact cttatgcagt tgttgagcgc gacaattacg
      121 gcgatcctgt cgcattgtgg ccaattttac caaataacat tttgccaatc ttatacaaag
      181 gcgaattata ctaccgtgtt ttcacaggcg actacgcagg cgactattca tcgtccgaaa
      241 tacttcattt caaacttttt accaaagacg gaattaaagg catcgaccca atcagcaaca
      301 tgtctcaggc cattggtttg ggtctggctg ccgaacgttt caatgccgaa ttctttgata
      361 aaggtggaca cataaaagga gtttacgaaa ccgaacaatc gttgggcgac gaagaattta
      421 atcgggttac tgcgcacatg aatgccgcta aaaattttga aactccgctg ctcgaatacg
      481 gcattaaata caaacaaatt accatttcgc ccgaagccgc acaaatgatc cagcagcgca
      541 catttaccat tcaggacatt gcgcgaatat tcaatttgcc gcctcacatg ctggccgaac
      601 aaagccgctc cactttcagc aacatcgagc atcaggatat tcagtttgta aaatactcca
      661 ttcgcccatc ggtaaaacgt tacgaatccg aaattgagga caaacttttc ttcgatggcg
      721 aaaaagattt tctcgatacc aaattcaatc ttgaaggatt attgcgcggc gatacacaaa
      781 cccgatcagc attttaccac aacgccattc tcgatggctg gatgaaccgg aacgaagtcc
      841 gcaaccttga aaatctcgat aacggtcccg tcgaactcga cgaatatctc tatccatcaa
      901 acatgaacct ggcagaactt cttttaggtc agcaacaaca aaaaaataat tcacaaccgc
      961 aaaatatcga caatggaaac taaaaaatat acattcggaa ccgtccgcgc tttcgatcct
     1021 gcaatggtcg aaaaaacacg aaccatcgag tttgttattt caacaaacac ccgcgaccgt
     1081 cacggcacca ttttacctgt cgataaatgg caactcgata atttcaacgc gaatggaatt
     1141 gtcggttatc agcacaacgt ttatggcggc gacctgtgca atgctcccga cccagatgat
     1201 gtcattggtt caggtcgcgc atggatcgaa ggcgatcagt tgattggttc agttacattt
     1261 gaacctgccg aaatcaatcc aaaagccgaa aaaatatttc ggaaagtcct gtccggaaca
     1321 cttaaagcca catcagtagg ttttaatccg attggcgatg gtgcctgggg tattggagaa
     1381 gaatccgaac gcggaactaa cccgacttac tattttgccg ggcaggaatt acttgaattc
     1441 tccattgtca acattccttc gaaccccgac gcgttgcgcc gcagtttgcg caaccaggct
     1501 tccgatgcaa tcgcttacat ctaccgacaa ctgaacggcg attaccgttt ttcccaaatc
     1561 gaagaaatga ccgtccgtca ggtaatcgat ttacttgata tgactcctga acaacgggca
     1621 ttatcggtaa aagtagaagt tgaaattgaa gaaagcgacc caaaaccgga cgaattgcct
     1681 gtaattccag aacccgaccc acaaccttca acatccgacg aagatttagt agtcgctaca
     1741 cgtgcaaaac tggccttaat ggctcaataa taacaattaa tttttatcaa aatggctaaa
     1801 aaatcatttg aaatccgtaa agagctgggc gaaaaaacca accgtttcga ggaacttcgc
     1861 gttaaggacc agcgttcggc tgaagaacag accgaatttg acaatctgcg cggcacaatc
     1921 cgcactctca ccaacgatct cgacgatgct gaattgaccg aggccggtaa aaaacgttta
     1981 gcttctcagg gattgagcaa tcaggaacgc gaacaagccg gaaactactc gttccgtaaa
     2041 gcaatttcag atttcgttaa ccgaaacggt aatctcgaag gtttcgaagc tgaaatgcat
     2101 caggaagctg ttcgcgaagc aaaagcaaac ggaaaaaccc tgaagggaat cggcgttccg
     2161 atgatggttc tcgaaaatgc ccgtgctgca accggacaaa acaaaggaac tgcaaacgat
     2221 gggggtaacc ttattcagga tgaaccgctg gtttacatcg aagcgttgcg aaatcgtttg
     2281 gttctggttg gtttgggtgc tcgtttcatt accggattgg ttggtaatct tcctttaatc
     2341 aatggcggta ctttctcagc agaatggtac ggcgaatccg atcctgtaac tcctggcaaa
     2401 ttatcattac caaagaaaac gatgtcgcca aaacgcttat ctgtagccgg tgcctactct
     2461 ttggaattga tcaaccaaaa ttcaatttct gtagaaatgt tgcttcgcga tgaattgatg
     2521 aaagctcata ttgcaggaat agaaggagct gcaatcaatt cggctacaaa cggaccagtt
     2581 ggagtattga atacttcagg aattggtgcg gtagctggtg gcgacaatgg tttggccgct
     2641 gcatggtcgc acattgttga tctcgaaagc aaggtagcta ttgctaatgc cgatttaggc
     2701 gcgctggctt acttaaccaa tgccaaagtc cgcggaaaac tcaaacaaac ccttcaggct
     2761 gctggagtac ctggatttat ttgggacaaa gagggcatga atggctataa agctgctgtt
     2821 accaatgctg taccttccaa cttaactaaa ggaacatctg aaggtgtttg ctcggctatt
     2881 gtttttggaa actggaacga tatgatcatt ggtcaatggg gtggatacga catggttgtc
     2941 gacccttata gcaaaaagtt gaatgctgaa gttgaagtag ttctgcattc tttccacgat
     3001 gttgccttgg caaaccctgc atctttcgct gcaatgaaag acgttctaac cgcgtagcat
     3061 ttgttttttc atatagggta aattttcctc cgggcattgc ttgcttttgc ccggaggttt
     3121 taatcccgaa actcgtatca cgtaacccgt aacattttta aaatgaaaat aaaattcaaa
     3181 aaatcggctc ccggatttgc ttatttcgcc ggagatgttg ccgatatcaa tcccgaagca
     3241 gccgcaaagt tagttgacga tggttttgca atcatcgttc ccgaaaccga aggcgacgat
     3301 aatgaattgc cggaagattt gcccggacgg ttgatccttt tcaaagaagg cattaaaacg
     3361 ctcgatgatg taaaaaagat caccgattcg ctcaccgaaa tcaaaggcat cggcaaaaag
     3421 ctggcttccg aaatagttgc ttacatcggt aatcttcaat agtaaattgt aaatcgttaa
     3481 atcgtaaatc aatgtatatt ctcattatac cggcaaccac gcttcccgtt tcagttgaag
     3541 aattgaaaca agacattggc atttttcaca ccgaaaaaga tagcgtttta tcgatctatc
     3601 tgcaatcggc tgtcgattat gccgaacgtt ttaccggtcg ccagttgatg cctgctgttt
     3661 gggaattaca aatcgatact ttttacgaat acctgaagat ttcgaaagct ccgtttatcg
     3721 acttggtatc tattaagtac tacgacgcca acaacgtttt gcaaacgctt gccagtggaa
     3781 ccgattttga tgtttgttcg tccgacccgg cgctcattca tttcaaaaca gatttcacat
     3841 tgtaccaccg ccccgatgcg ttgcaaatcc ggttcaatgc tggttatgct tctgctgaag
     3901 tggtgccgcc aatgatcaaa gctgccatta ttttgatggc tggtaaaatg tacgacagtc
     3961 cgattgattc ggttgagaat ttgccaaaag cttcgacaaa ccttttaagg caataccggc
     4021 tatggcaaca gaattagtaa gcgtcaaaga ttttaacgag ctgatcatgt ttcgccagcc
     4081 atctcgcgac aaatcgtcaa aaggtcaggt caaagaaaca tggaccgatt accggaaagc
     4141 cctggttaaa gtcatcgaat cgggtaacga cgaacaaaag gaaggcgacc gcatgaattt
     4201 gcctggtgtg ctaaccattt caggtcatta cgattcgggt gtaaatacaa ctttcagagt
     4261 tctttggaat agtgtagaat atgcgattac atctataaca ccggttcaaa atcgccggtt
     4321 tatgaatgtt cgtgctgaaa gggtttttga ataatggcaa agcaaccggt aacaatcgaa
     4381 ttcacaggaa ctgaaaacat tcaggaaatt ttcaacaaac ttcctgaaca gtacgcaaaa
     4441 aaaccggttc aggccgcttt cagaaaagct gcaaaaccat ttatcgctgc tgtcagatca
     4501 aatttaccta gccgtttatc gtcgttgcag aaaattataa acgtggtgaa taacaaatcg
     4561 gctggaataa ctgccggagt tctctctaaa aaagcaatgg taaccctcaa agataagcgc
     4621 gaatacgatg ctttctttcc ggtgtactgg tcaaactacg gaacttattc caggcgcgat
     4681 gcctcccaca agtttacgca aaagcgaaaa gcaaaaacag caaaccggaa gggtggtatc
     4741 gttccgctca ggtttgtcga aaaaagctgg gatcaaacaa aagctcaggt cgaagaaatc
     4801 gcacaaaggg aactgattac gcaaacagat aaatttttaa aaaaacacgc aatcaactaa
     4861 gttatgtctc catttgaaat tgtatcgaca attgtggcaa ttctcggatt agcagttctg
     4921 atcctgaaag tgtggatcaa atcacaaaca gatatagcgg caattaaagt tgagataaac
     4981 gcaacgaata atcgtcagaa aaatatggaa aacgacctga aacaacacaa gttggacgat
     5041 aattttcgct acgaacaaat gagaaaagaa aatcgagaag atcacggaaa gcttttcgaa
     5101 aagattgaca aaataattga aaaacaagat gataatcgac gcgctcatag caacaattaa
     5161 ccctattgtc gattgctaca ctgcaattgg agatttaaaa gcggaattgc cattttcggt
     5221 tattcaggta gctcctgaac ctattcgcat taaagacgga attgttggct atacgcaaaa
     5281 agtttcggtg gcgcttgtcg ataacgatgt cgatcgcatt gaagaaaaca ccgttgcagt
     5341 acgcgcagct atcgaagcat taaccgggct ggttgaagaa tgccaaatca attcagtcat
     5401 gttcgaatca gaaaacggag tcattttcga tcctgaaaca caaacatatc aaaactcttt
     5461 cgaattcaca gtcgacacga atacaaggta attaaaaatt cataactcaa aattaatact
     5521 atggaaacga gaacgatggg ttacaacata acccttaagc tgaacagcaa aaaagtgatg
     5581 ggtactaccg acaacgattt taatatcgat gccgaaacaa aagaatcttt gattaaagac
     5641 gatttgggaa aatctcaaat cgactattca ggctataaag ccacgtttgg aatcaacgga
     5701 ttgtcgatga ctaccgacga aactgaacct acaaaattga ctcaaaacga cttgatttca
     5761 gcaattgtga ataaaacaaa aattactttt gtgtattcac gcgttggctc aactgcgacc
     5821 attgaaggaa atttggttat tacaaagtat agtgaaagtt caagctcaag cgatcatggt
     5881 aagtggactg taagcgccca ggttgacggt gatttgactt ttggaacttt gcatgcataa
     5941 atttttattg aaggattgat ttaattcaat ccttcagttt ataaatcctt attatgaaaa
     6001 acgatcacgt agaaattaac ggaaaaaaat acagggttga attcaattgg aatgccatta
     6061 ccgattttct cgaatcagaa ggtttgcaat tatcagacgt tgacgatttg cgcgagttaa
     6121 aaccgcgtca ggttaccggg ctggtttatg ccggagtatg cgaaggatgc cgcctcgatg
     6181 gtaaagagtt tacattctcg aaactcgaat tcggtgcaat ggttaaaccg ccacaaatcg
     6241 gtgaattgct cttgatttat caacggcaca catccgtcaa aaaatcggag gtggctgagt
     6301 ctgtcgaagc acaaaaaaaa agatttcaga acccgtttcg gtcgaagaaa tcttagaaaa
     6361 agcaattcgt atttgtagcc tgaagccttt cgagtttcgt ttgatgcgtc ccgccgattt
     6421 tttcatttac ctggatgcaa tgaacgcaaa ggtaaacgac gaatttaagc gagacgccga
     6481 attaaccagg ctgcaaacag caacattgat caacattcag ctaaagaaag aggaccaaat
     6541 aaaaccgcgc gatttgtggc gcttcgaatg ggacgatcag gaaacagaaa acgaaagcgg
     6601 tttaaccgaa gaacagatta tcgaacacaa caaccgattg tcagcagttt ttaacaacaa
     6661 agattaaacg atatggccga aacagtaacc aacctaaaag tccgctttgg tgccgacacc
     6721 aagaatttta aagccgattt agaaagcggg aaagccgccg tcgataaatt taccggagag
     6781 gcaaaagatg ctttctctga atttgcttct gtattcggaa taaatatgaa cgaagtttcg
     6841 gctaaattca gttctgtaca aaaaagcctt agcctaattt caggaggatt gaaaggaact
     6901 actgacggat taaaaggaac cactgaagct gttagaatat ttggtgtatc gtggaaagtt
     6961 gcattgggtc caattgctct tgtgattgtt gtaattggtt cattagttac tgcattaact
     7021 agattttcag ataatgctga aaaagtaaaa gtagttatgg ctggtatagg tggcggaatt
     7081 gatgtgttac tcgaccgttt tgcaaaattt ggaagtgcta taattgatgt gtttagcggc
     7141 gactttgttg gggcggctca aaaattcaaa gaaacgttca ctggaattgg tgctgagctt
     7201 gaaaatgata ttcggctaac ggcaatgctc aaacgggcaa cgctcgattt agaacgtgaa
     7261 tcaaaagtat tcgatgcaca acgcgaagcc gggatgacta aaattctaca actacgcgaa
     7321 cgggaaaagg atcagacttt atcagctact gaaaggctaa aggccaatgc tgaagcaaat
     7381 aaaattgagc aaacactggc agagcgctcg cttgaactgg ccgatcagga aacagccgca
     7441 acgctcgata atattgatgc gcaaacaaag aaactacgtc tgagtcagga agctattgtc
     7501 ctaatcgaaa aaataaaaaa cggaacaatc ggcgcaatgg aagcacaaaa agcagccgat
     7561 gaacttgatt tgggagataa agacggtaaa gaatcgctct ttgccctggt tgatgcgatt
     7621 gtgaagcgcg aaaaagaaca gcaggaaata ctgcaaacac ggcttcgcct tcagaaatca
     7681 gaaaacaccc ttaaaaacga gattttaaaa caggaaatag acggcttaaa ttcacttgct
     7741 tcattaaaac aacagcaatc acagaacgat aagaaaacag atttggagcg tacaaagctg
     7801 atactcgaag cctccgaact tcagaaaaaa gccctccgaa tcgatttcga cgccaacaac
     7861 attagcaaag aaaaatacct ggccgaactc gataaacttg ccatcgaaac tgaagaaaaa
     7921 ataaaagcca tccgcgaaaa acttaacccg aaaatggaaa cactttcggc cataaagtta
     7981 accgtcgatg acaaaacggg caaaatagcc caaaccgaaa taccggcccc aaaaattggc
     8041 gaactcgaaa cctccaaact agaagccaaa gccgcaaaaa tacaatcgat ttacggaaat
     8101 cttaaatcta cggttgtcga tttctcatca gtttttcagg aatcaatgac tggattagca
     8161 gtaggttttg ctgaatcatt gggaacaatg cttactggtg ctactggatt tggagattta
     8221 gctgctatgg ctggagctgc tttaggtgat atggctattc aagttggcaa aattgcaatt
     8281 caaactggta ttgcttcatt ggcaatatgg gaatcattaa aagtacccgg aggaggagct
     8341 ttggctattg ctgccggtat tgcattggtt gctttaggaa ctgcggctaa agctgcttta
     8401 agtaatgtgg cttcaggcaa aggaaattat tcattagggt catcaacttc atcctacggt
     8461 tccggctctt atggatcatc atcgtcgccc gtgcgttcgg ccccgcaaac aatttatatc
     8521 accggagaat tccgccagcg cggaaccgac ctggtggcag tcatcgacaa aaacacccaa
     8581 cgccgcgtaa tcggcactta aacataaaac acgccagtca tcctgaattt atttcaggat
     8641 caggacctca tattaacaac agatgccgaa tttattttgg catgacaaac gaataaaatg
     8701 gcatacggat taaaatacat actacgctac gaaacccaac gattcggaac cgacatccgg
     8761 gtattgatct acgaaaaaga ttataccgga ccatcgcaac caaaacgcat cggcgccgat
     8821 catatcaggc tttcgcgcga taaagacggg caaatatgtg gtacaaccct agatttttcc
     8881 attcaggctg ataccgacat ggaatttgcc aactttttta cactcgataa caaagcgcac
     8941 aaagttgaac tggaattcaa ttggggcgta gtttggactg gcttcgtagt ttccgatcag
     9001 tattccgaac cattcgttgc gccaccgtac gacgttcagg ttatggcgac agatggcctc
     9061 gggcttctta aaaacgaaaa gtttgagtta accggcatgg tatcgcgttt tgaagcaatc
     9121 cgttattgca tcgataaaat tggcattgcc ctgccatacg caatcaacat caatatttgg
     9181 gagcaaaaca tgaatatctc tggtcataac atgctcgacc agctttattt tcatgccgaa
     9241 atactgagag ataaaacatg ttatgaagct attcaggcac ttttgcccga aaacgtgttt
     9301 atcaaccagg caaatggtta ttggatgata gagcgtccaa ctgatcgtac cttgccccgt
     9361 tatattttta atgccgatgg aactccatcg aacgaggaat acaactggca cacattttta
     9421 cgtgaactca atacggtaca gtattcagaa attcataggg gcagcttata tccaattgga
     9481 agtctaacgc tcgaaatgca atcggcctgg cgaaaattta ccattaagca aatttttggc
     9541 aagcgagaaa gcttttttcg taatcacgat tttaaagccg gtacaaccta ttgggaaaat
     9601 gtagggttag gtgccaattt ttacgcaaaa aatgtaaacg aagagaattt aggagttctg
     9661 gccggttatc aaaattcata tgtaaatcac ataagccaaa gtatagatgt aatttcaaca
     9721 agcgccataa cgcttgagat aaattatgct ttagttggag tgtcgccaat agggggaaat
     9781 atcaattttt cgaaattcaa tccaaacgtc aaatttcaac tcaaattagc cggtacatca
     9841 ggacagaatt attatctaga tcaacaaggg tggaatacat cagccaaaga aattttaatc
     9901 gaaaaagtac cctattcatt cacattcgaa gacatcaatt ttcagtcaac aaaaataaac
     9961 gcggcaaagc ttcccgaatc aggagtttta accataacca tttatcaggt aattccaaat
    10021 tcaaatgtta aagcttatga aaattgggcc gatgtttaca aaggattatt cgttaaaaaa
    10081 gtaatatttt acaacaacga cataacccaa tttaacgata gcgaagaaac tacagttatc
    10141 cttaacgaaa atgccaccga atcggaaaaa acaatagaac tgaaaccgac ggacattccc
    10201 aactacgaaa ataataattt gtatttcgat aacggaaact acgttaaagt taatgggaaa
    10261 tacataccaa caaaaagttg gggctcgcag caaaaaacat acctcgaata catgtcggca
    10321 tacatggcct atctgtatca atcgtcgcgt tcggttttga aaggtgaaat ttccggctgg
    10381 ctcgaattaa acagctatgt agttcactac cttacaaata aaatttacct gatcgataaa
    10441 ggcacatgga acgttttaga tcacacgttt acgctcgacc ttatagaatt gccagcaggt
    10501 gaatttattt acgataatta cgcccaaaat ttaccagaac cgtttgttcc acatgtcgat
    10561 atttcagatg taaacgattt tattgtacca atcgacggca gttttgaagt tccgctaaca
    10621 ggcacacaca cgccgttaat tgttgcaagg gcgcaaacaa tcgacccgct aaaactcgat
    10681 tttgattcag gaaccccgca attctccatt aaagatgctg ttgtagaaac caacgtcggc
    10741 gacaatgcca acgctgtaaa aattggcgct ggcgaaataa taagccacca ctacgccgcg
    10801 cttgatcgta cttcaatacg attcatcaaa gaagcaaacg aacagtacga cccaacccgc
    10861 gcctgggaca ttgccgaaac aaacattgag ttacccgaca acgacggata ttttctatat
    10921 gtaaaagtac cattagccga acaattacca gaatgatacg aaccagcgaa atattaacct
    10981 caaaaaccca tatcgagcca ctgcgcgatg aaggatttgt gatttatcca attgcctaca
    11041 ttggtccggt aataaacgga aagcgcgaag cctattcgaa agggggaaat ataaaaggta
    11101 caacaaccat cgacgaaaaa gtaagactaa tcgacccgtt tacatttaca ccaatgggcc
    11161 aggccggtta tttatcgggt caatattttg accaatacat agataatgga acgggtagag
    11221 caataatatt tccaataaaa caagagaaaa ttataacaca aatcgcacac ggatttgaat
    11281 ttggaaatgc tattaaacgc gacggggctg gcgcttggat acgggcaaaa gctaacaaca
    11341 gggcgaacgc gggcactgtg ggaattgtat caaatgttat tgacgctgat aatttcgaat
    11401 accttcccgg ggggctattg cccggtgatt tcgtggacgg cgcctgttat tttttatcac
    11461 cgtattatga cggtcaaatc ttcgtgcaaa gcgatccgga agtgtggacg gtcggacaaa
    11521 ttcgtgaatt tatcggcaca ggtactgcac aaggtctcga aattgagatt gatttgggcg
    11581 atgaaattat tgatttgcag gatttaaaag gcccacaagg cgctcagggt tcacaaggcg
    11641 ctcagggttc acaaggcgct cagggttcac aaggcgctca gggtaatatt ggctcgcaag
    11701 gttcacaagg gactgctggt attggtctta accttttggg agaaaaaaac acgcccgctg
    11761 atttaccgtc ttcgggaaat tcggcgggcg attgctggaa cgtagcttca gacggccacc
    11821 tgtatgcgtg gaataacgct aattgggttg acgttgggca aataaggggg ccacaggggc
    11881 cacaaggctc ggcaggttct cagggtaacc aaggcgcaca aggtagcggt ggcgctcagg
    11941 gaaatcaagg tgtacagggt gccggaacgc aagggccgca ggggaatata gggccacaag
    12001 gagttcaggg aagcgatggt caacaaggct atcaaggaac gcaaggtatt cgaggttacc
    12061 agggaaatca aggtaaccaa ggaatccaag gtgttcaggg aaatcaaggc tatcaaggct
    12121 cacagggagc aacatcaacc gtgcaggggc cacagggcaa tcaaggtagt cagggctcac
    12181 aaggagttgt aggggctgga tacactgcta gttcatcgag tagtgtttca gcaggaacgg
    12241 gatttaagta tttcacggtt caatctggtt tagcttactc ggcaggagca agagtacggg
    12301 cttatacggg aggttccgtt ataattcttg agggtatatg cacttcatat acaggaaatc
    12361 agttaaccat atttgttgat cgttcagtag gttctggctc tgccagctcg tggactatca
    12421 atttagctgg ggaggtcgga acgcaggggg cacaaggata tcagggtgta caaggcgccg
    12481 gttcacaggg gccgcaaggc tatcaaggtg tgcaaggaac acaagggccg caaggcgttc
    12541 gaggttcaat aggaatgggg ctacaggggc cgcaaggcta tcagggggca tcactttgga
    12601 cagatgctgg agcttatata tatcctactc aatattctga cgctgtactt attggcgctc
    12661 aaaccagaaa ttatggcact tcaaatattt tagaagttat tggttcaagc gcattttcag
    12721 gcatcgtgaa tttgatgtcc gctttatttt tatctggaaa ttacattgct tttgccgacg
    12781 cacattcatt accgggcggc cttgaaaatg gtggaattct ttacacagac ccgtctgatg
    12841 gattacttta ttataagcgt agcaatgcta aagggggtgg aacttattta ttatcaaaat
    12901 aatactttaa aatgaagata tcgctattca cgccaacaca tgattcaaaa tatctatctg
    12961 aagtttacga atcaatcaaa gatcaagatt tttttgagtg gctggttatt tataataacg
    13021 gggcaaaaga aatcgatttt aatgatatcc gggtaaaaac tatccgcctg accgacgcgc
    13081 ccggatgggt tggcccgtta aaagcatttg cttgtgaaaa ctgcaagggc gatattcttg
    13141 ttgagatgga tcatgacgat tggctacacg gtgatgcaat agatgagtta aaaaacgcat
    13201 tcaaagaccc tgaaattggg tttgtatatt caaacactat ccacgacaaa gaacatcatt
    13261 acagcgaaga gtacggatgg aaatataggc cgatggatgg acaatgggaa gaacatatct
    13321 cattcgctcc tacgccggat tcaataagta aaatttggtt tgcacccaac catctcaggg
    13381 cattccgcaa atctgtttat gagcaaattg gaggctataa caaggaaatg cgggtgctgg
    13441 acgatttgga tttgatgtgc aggatgtatg ccgttacaaa attcaaacat atcgacaagg
    13501 gcttgtatta ctatcgcgaa cacagcgaaa atacttattt gaaatacaat gccgaaattc
    13561 agaataacgt attcaggatt catgatcagt acatagaaag cctctgcgaa cgatatagcg
    13621 atttgaacgg cctgaaaaaa gtagaattgg gcgggcgcat tgccgcaaaa ccgggctatg
    13681 agactgttga cctaaaagat gcagacgtaa tttgcgacct aaatgagcct tggccttatg
    13741 ccgatagttc ggtaggcgtt atccgcgcct ttgatgtgtt tgaacacttg tccgatccgc
    13801 tgcatacaat gaaagaagtg cagcgaatat tggctcccgg aggctggttg atttgccagg
    13861 ttccctcaac ggatggccga ggtgcttttc aagatcctac gcataaaagc tactggaatg
    13921 aaaattcgtt cttttattat acgaagtcgt tttttgcaaa atatatcgat acgccggtac
    13981 ggttccaggc atcaaggcta tatacaaccg aaaaaaatca agatcaggta tgttgggtgg
    14041 tagctcacct ggtaaatcta aaagacggtt ttcgtccggc tggattggtt gaaatttaac
    14101 actttaaact atggcagggg gaagggcagg cacaaacata gtcgtactca acgggccgca
    14161 aggcgctcag ggcatacaag ggcttcaggg gccgcagggt aatatcgggg cccagggttc
    14221 acagggtagt cagggcatac aaggccaggc aggtaaccaa ggcgcacaag gtagcggtgg
    14281 cgctcaggga aaccagggaa atcaagggcc gcaaggcgcg caaggtaacc aaggccaggt
    14341 aggtaaccaa ggcgcacaag gtagcgatgg cgctcaggga aaccagggaa atcaagggcc
    14401 gcaaggctca cagggcaatc agggtgctca aggtgccgca tcaaccgtac aggggccaca
    14461 aggctatcaa ggtgccgcat caaccgtaca ggggccacag ggcaatcaag gtgccgcatc
    14521 aaccgtacag gggccacagg gtacgcaagg ttaccagggg gctgccgcaa cattaaccga
    14581 caattcagta acatacgcca aaatagccgc cgcattgaag caaaaaaatg cggtaaccgc
    14641 ttcggttgat ctttcggcaa acggtatagg cgaaataacg ctatcaacaa atacagcctt
    14701 tagtttctcg aatttcgagc taaacaaaac gtatttgctg aagattacag caaacggata
    14761 tacgccaagc tggtcagccg ctgcaaagca tgtacctgtt gaagggaatg ctacattcgg
    14821 aacttcaggg gtattctacg tttcattaac ctgtatagac gccaccgcag ggagcgaaaa
    14881 gttattaacc ctaatcatga aaggagttta atatgttgac tccaggaata ggaataactt
    14941 gcaaaccatc tgctttaagt gtagaaggga aatatcaagt ggcttgtgat atttctactg
    15001 gcatttactg ttctagtgat tacggtcaaa cattctcact taaactttca attgctaaca
    15061 aaaactggtc aggagttgga attagtgcaa ctggtcagta ccaagttgct gttactaata
    15121 atacaatgac ttatctttat ctttcaacag attacggaaa tacatggaac acgacaacat
    15181 tgttaataca aagtttgtcc gtttgtataa atgccactgg tcaatacata actacaggta
    15241 tatcaggata cataaaacgt tcaataaact atgggtcttc atttagttct gtaacacaag
    15301 caggaatttc tgctggattc tcatgttcat caagtggaca atatcaggtt gtagttatgc
    15361 aaccagggta tatttttatt tctacaaatt atgggttaac atggtctcaa aaaggtaata
    15421 gtagatcata tacaggagtt tcaatgaatt cgaatggaac ttatatcatt gctgctgtat
    15481 cttctaacta tttacaattt tcatctgatt caggacaaaa ctggagtgga aaagttggat
    15541 caaaatcatg gcaatctgta tcaataagtt caagtggtca atatgtattt gcagtagttt
    15601 atagtggttc aatttggaaa tcagaagatt atggtcaaac ttggtcagag attgtaataa
    15661 caaaaaaatg gatgtccgta actgttagtg caacaggtaa atatgtaact gccgtggctg
    15721 attcttcaca aatttatgca tcatccaatt atggccaaac attttcacaa attaattcat
    15781 caagagcgtg gagtgctgtt gcaattaata ggtttagtga cgtataattt aaaaaaaaaa
    15841 tgaaacattt aattaaaaac ggccaaatta ttcaatcagg attacccgga acttttaccc
    15901 gcgaaaatgg tgaagggttt tggggaggtt acgaagaacg aacagatata cattttatag
    15961 acggttggag agatgaaata ataccagaat acaacccaat actagaaaaa ctaagcgaac
    16021 cgttttacga cactgataac gacgtagtaa cttacgaagt gattgagcga aacgatttac
    16081 cgccgctcga aacggccaaa gctgacaaaa ttaaagcgat taagctatac gcccgcgacc
    16141 tattgtttgc aaccgatttt tatatagttc gtcgcgccga aacgggaaaa tcagtaccac
    16201 aggaaatctt gaacgccagg caagctataa gggtttggaa cgacgcccag gaaaaggcgg
    16261 tgttggcatt aactacattt gaagaagtag cgagttttga aataaagtat taaagagccc
    16321 ccggctccac taacccgaaa ctcatataaa gattttttaa aaataataaa atttcacaaa
    16381 atgaaaacac ttgaaaaaat tgtacagttt ttttatggac ttaaaacatt gactttagag
    16441 ttcatgaata agttaaaaac agttagagaa aaaaatattc tcacaatcat tgcaattatt
    16501 tctattgcgt tatttgtcat tttttacatc tatggcaaaa aatatgaact tcaagttgta
    16561 cccattggta tgttgcaaaa agtaattttt gccatgattg caggaacaac tgggccactt
    16621 acggcattaa tatttcttaa atatggccgt cctgattctc aagatgcttt gagttcagat
    16681 acggaaggcg gtattaataa tttaaccgac tgggaaagag tcaaactatc gatgttctgg
    16741 atcgcagcat tcatcattcc attctcaatt gctttactcg catagttatg aaaccgttta
    16801 ctttgatatt gctgatatta atagcgtcgc aggcgtttgc tcaaacgctt gaaatgccaa
    16861 caagcccact tcgagacaaa ttggttgcta ttgccgatag ttatatgggc attcgcgaag
    16921 ctacagggcg caacgacggg ccaaaagtta gaaagatact tcgtaatgta ggactacccg
    16981 aaggaaacgc atggtgtgcc gctcttcaag ctcaggtaca tgatgattgt aatcttccaa
    17041 atccacattc tgcttactgt cctgactggt ttaaatcaaa cgtagttttt gaaaaagatg
    17101 aaatgagtat cgataaattt caggcaaagc gcggacaagt atttgggcta tggattgaat
    17161 ataaacatcg ggttgggcac atgggaatga ttaccggaga atcgagattt agttattaca
    17221 ctgacgaagg aaatattaac agcaaaggat ctgacgaagg tgaaggcagt gacaaatata
    17281 ttagaagcaa acgatctatt catgtcatcg ctgactatgc tatgtcgcgt gaagaaaaga
    17341 acctttattt aggtaaacat cgaaaaataa ttctgaaaga tgcagattga acctccatac
    17401 gaaattaatg atagcgtttg ccatgtctta aataataagt tggtaggatt agttattgac
    17461 tggacttatt caaggcggtc aaatagtttt catttcatcg tgacgtttgg ccccggtacc
    17521 gctactgtaa aacttgacga aaaagaactt caaaaagctg aaaaatgaac atcacaaatc
    17581 gcattgaaaa cattttgatc gccgcagccg ctgcaatcat tgcaatagct ctaaccgtcg
    17641 caatcatgga aatgaccagc attgctgcct tgaaaaagga aaattcgagg ctgattaccc
    17701 aggctgataa gcagaacgct gtgatttttg aactcgccaa aatagagaaa tacaaaattg
    17761 aaaatcgttt tgaccaggtt aaagcaaaag atggtcaagt cgttatgtcg ctcgataata
    17821 aactatcagc gctgtcgctc gacagcatta aacctatgac aaaaccaaag gtaaagaaaa
    17881 cgttttggca aaaactgaaa ttttggtaaa atatataaat agtatataaa tgatttgata
    17941 tttattttta actatctgat ttataattta ttaaaatatt tatcatgatg tctggaaagc
    18001 gtgtgtacct ctaaagggta ccgcgggttc gaatcccgct ctctccgctg aaaaccaccg
    18061 ttttacggtg gttttgtttt ttataacata gccgtttatt ttcatttgaa cgccacaaaa
    18121 agtacattta tataaaattt ctatataaat agtatataaa agaatggcaa catttaaagc
    18181 agttatatta aatggaaagg cgtttgagaa gtcggacggt accacaaata taaaaattcg
    18241 catcactcat aaaggcaaac aggattacgt ttctacgaaa tattttgttc tggtgtcaac
    18301 cgaaaaaaaa tcgtatttcg attcaactac cggaaaagcg aaaggaacat ccgacgccaa
    18361 attcataaac gatcagattt cggtattgat tgcttcttat attaaagccg aatccgctct
    18421 aggtgactca tccgaccaaa tgactgttaa gcaaattaaa aatcatctta cgcagcgaaa
    18481 atctgataaa tacattgata ttattgaatt caccgaaaat ttcatttcaa ggctcgaaaa
    18541 gcttaaaaaa gacaatacgg ctcaatggca ccgttccatg ttatcggcac tgaaaaactt
    18601 tgtgaaaaat acgaaattga acgttctcga tgttgattct gcttttctta ataggtttga
    18661 acagtacatg ttgaagggag ataaaaccac caatccggtt cgtgaaccga tgactgaggg
    18721 gggtattaat aattacatgc ggtcgttccg aagcattttc aacaaaatga aggacaaata
    18781 caacgacgag gaaaccggca taattcgcat tgcaaaaaac ccgtggaagg tatataaggt
    18841 aaaaaccccg aagcggagac aacagggcaa aaacatgacc gttgatgaaa tgaaaaagct
    18901 ggttgcttat tcgagtacca acaagggcaa acagtttgcc agggacatgt ttctgctcca
    18961 tttttgcgcc attggcataa acacgaagga cctttatttt atgccaaaac aaacaggccc
    19021 gcgcctggag ttcgaccgat ttaaaacaca gcgggcatac tccattaaaa ttgagcccga
    19081 aatggccgaa attttgaccc gttacgagtc ggatagtcgg ttggtaaaca ttcacatcaa
    19141 gtataaagat taccggatat atacccgcca gattgacgta tttttaaaga aagttgcagc
    19201 cgatttagaa attgaaaagc ctatttcgag taactgggcg cggcacacat gggcaacgtt
    19261 tgcccggata aactgcaaaa taccaaaaga cgatgtggcg ctatgtatgg gccacgaaga
    19321 tgccgacaat aaagtaaccg acgattacat tacctacgat tactcgataa ttgaccgaag
    19381 caaccgaaaa gtattaaact taatatttaa gaaaagcgat atacgaaaat aaccgacgaa
    19441 ttaaggaaaa cacgataatg ggggccatac tcattgttat cgttttgcta tgctcgctgg
    19501 tatattgggt ttttaccggg gtgtggccgt tgtagtatca tattcctgac accgggaaga
    19561 tgatcaaaag ttgattggtt ttccaaattt ttcagcgact gttttcattt catcgaacgt
    19621 tattcgatat gccgatcctt tcattccatt attttgaatt tgctcaaaat tatcttcaga
    19681 tcgcacgccg taaatttcta aactaactcc aagtttgaat tttccggata caacaattac
    19741 gctatccttg cagaaaaaat tgaggtaata cgggtaattg cgttttttag tattttgaat
    19801 gtctgttttg actataaaag tttccttatt ttgttcgcaa actatttcgt tttcagtaag
    19861 aatatcaacg catagtttca aattctcggc ttttggtttt ctagtttcaa taattatttt
    19921 gtttgcaatc tttttttctg gactataata agcatcatcc tgggcaaata ctgctgaagc
    19981 gaaaaacaat aaaattaaag ttgtaaataa agttttcata gtaatttaat tttcagaggt
    20041 ttttaaaacc ttgcttaaat ttttcgaaac atagtaagat ttcgaaatag tttgcttcgc
    20101 attgttaagt ataggatttt taattgtata tgtgcaattg tattttatag ctaaaatgga
    20161 atcgttaaca atttcatatt taaaagcttt tagattattg attctcaatt ttgttcttat
    20221 ttcagaccaa tattttaatt tttcagcgta aaaatctgag tagtccattt ctaaaatatg
    20281 agtatatgaa ttaacgagag tatcaagagt gccattaatg cttttaaaag tttttatttt
    20341 atccccactt aaaattttaa ttgtcgaatc aacttttgct ttaaagaagt tagaactatc
    20401 cttaccggta attttctgaa cttcttttaa ttcatgaatt ttcagcgata aatcagtttt
    20461 ggtgttgcca atcgtttgct cataatcaga aatcaacttt tcgacttttg aaggactaca
    20521 cgaaaataaa ataaaacata acaatacaat taagatattt ttcatagcaa tagttaaaaa
    20581 tttttagcta aattaagtca tttcaagtta attatgtatt ttttatcgcc gaaaaatttt
    20641 tccaaactat tcggagccgg tttgtttata tgattcttca tttttcaaaa ctaaaccttc
    20701 tttcaatccc tcatatttcc ctttccaata ttgaacttgt ttttcaagat cgttgcaatt
    20761 aagacatttt cttttttcat aaatgccttc aggttcttga atcacatttt caaattctgt
    20821 caattttttg atagctaaat caaattctgg ataattttca ttgaagtatt ttttaatttt
    20881 ttctgttttg ccagggtatt tttcaagttt agaaaaatat tcttttcgta ttcctatctc
    20941 attagctaat aaatcattag cctttccaga taatactatt ttcaaatcaa taatttgttt
    21001 ttcagtcaaa ttcatgttaa aaaacatata aattaaatgt ctattaattg atactaatgt
    21061 caattaatag ttatatttgt accgttatcc ttgttcaaat atacgaaacc aaaacaagcc
    21121 taacaatacc gtaaaaccat tagaacacta aaacgctacc aaatgaaacg aactctaaca
    21181 ccacgcgaaa ccgaaatagt tgaactggta gccttcggat tgtcgcaaaa agagattggc
    21241 gataaattgg aaatttcgcc aaacacggtt gatgttacgg ttaaaaacat taaaacgaaa
    21301 ctcgatcttc agaaatctac cgaactggcc gcatggtact ttatcaacac ctaccacatt
    21361 acgcttaatc tttcgcccat taaacgggct atgatttcgc tgtcgtttct ggcgctcatg
    21421 gtggtgtcgc ttttcgaagg ttttaacccc gaacgatctt tcagaacaat acgcgcatcg
    21481 cgtgcccagg tgtcgctcac gttgagaagt gggcgccgga gcaatgaatc agatttttat
    21541 ttattaactg catagatatg agtgagatta gacttacacg atttgaatta ggccagatta
    21601 taagtactgc cgttgagatt ggtgtttcga gggctactga gaaaaaggct tttttgagca
    21661 aacgtgctgc cgacataaag tatggtcgcc gggcggttga tcgctggatt caggatggaa
    21721 aattacagcc tgtaatgcaa aaacgcaggg ttaaactcga tgccgaccgg ctgaatgaac
    21781 tttcgaaagt tgatgagttg tacggaaaat ttattggcga acctgaaaat gattaaaacg
    21841 atgagtgcta aaaaatctcc gatttcggta cgcgaagccg cagagcaggt ttttgccgaa
    21901 atgcccgaaa cgtttcatgc tattttcttc tgcgcacggg taaaagtacg cactggacgc
    21961 gctgcgctga tggacggtac tattttgcgg cgccttcgcg aagcccgcgc cgactcacaa
    22021 caattccagt atcggtgtat tgatgccgat aagtcgatat atcaaaaaat tgcgatatga
    22081 aaacaaaact gttactcccg tttcgcgtgc tgcggtatat gatgactttg cattttgttg
    22141 tgcgattgat gattgggttt ataatttttg taagtgttag gttagttttt ggggtttaaa
    22201 gttgaatttg tgtttttgaa atgggggttg cttttttgtg taaatggttt ttcgccccca
    22261 tttctttttt taaagaggtt gcaaaacggt tgcacttatt cggttcgatt ccgaaaactt
    22321 cacttatcct gagcttgtcg aaggaaaagt tcattaacat tgttggaaat ctattcaccg
    22381 cgatagtgat ctgaaacttc ctcgcgggaa cacaatcaaa gacacagatc aatacttccg
    22441 gtggaggccg tgtgaatcgg aaaccggttt gattactacg atatgcctga ttagctcagt
    22501 tggtagagct gctgtttgta ttcagcatgt aggcggttcg aatccgtcat caggctcatt
    22561 gttcgatgaa actattttga aactgtttgg acaggggttc gattcccctc acctccacgg
    22621 aattcgccaa ttctcgctaa tctgccgcgt tagtgcagtt agttcaactt ttgtcattca
    22681 gatagggtag gtaccaatac cctatcattt cgggggtgtt tggttttgac aggcagaaat
    22741 taggaatagc ggagagcatt aaacgccaaa cggcaaaaca gtaagtattt tcgattacga
    22801 agttaaagta gcagcgtagt tgaacggctg gccaccgaaa aaaggccaca cgcctgatta
    22861 gctcagttgg tttagagcga ccgccaatag taccttgata ttattggtag ggaggtcagc
    22921 ggttcgaatc cgttatcagg ctcatggcac aagtcagcga cgaccgacaa caacatgaat
    22981 tgaacctaaa gcgcaaaact aaaagcgcga tcaggaaaag gtaaaaataa gcctgatgac
    23041 acctggaaag aagcgaacag gacgcagcca aaacgaatca aggagtgaaa tgttgtctga
    23101 cagccgggaa agaccggcaa aacggagtgt tcgatttgca tggttgagtg aggcaagcgc
    23161 cgaaagggag ttgtccaaaa acctaaacat ttgagaagcg aggtttgagc cctcacactc
    23221 cacgacattt tatttttttt gacgcatttg aaatcatgtt tttgcccgga ttggtccacg
    23281 ccagtggtac ataaccgggc aaaattttta aaaaccaaaa tttatggaaa cgaaaataat
    23341 tatttgcgtt gcaattgtgt ttgcactatt tgtggttttc atgtttgtta ctgcttcgct
    23401 ggaagccaaa aacaacaggc tttcggctga aaatgacgat ctgaaaggcg aagttgaaat
    23461 aagggatatt cagatcgaga tactgaaaaa tataatttca gactctgaaa aagaaacacg
    23521 tttttagcat gaaacttaaa actgccgaag aacgcgctat tccgtttttg aaaaataagg
    23581 taaaacttga cgattataac aaaaatggaa tgtttaaaga tggatttttc aacccgattt
    23641 gtccgcctga ttacaatttt ataactggaa aacaagagcc tttaattact tctcagcggg
    23701 gtatgcgcga agtaagttta gccaggagag ttaacggaac gccaattttc aggaattagt
    23761 atggccagac ctcaaaaaac aaatgccgat tatttctcgc acgacacggg catgagaaat
    23821 gatttgaaga ttaaagccat tcgccgaaaa tttgggctgg agggttatgc aatctggtgt
    23881 atgatgctcg aagtactaac cgatagcgat aaccttgagc ttgaatacaa cgaaacggaa
    23941 ttggaattaa tatctgccga tttcgatatc gatgctgaaa aactgaaagc gataattgat
    24001 tactgtgtgt cgccgcttcg tattttagct gtagaaaatg gttttttatt ttccgaaact
    24061 cttaaaaaac gccttgattc tttatttcag aagcgaaaac gtcaacaaaa aatagttatc
    24121 gacgtcgata actcaaaaaa atatgagttt acgaatgttt tagctgttga taatacacat
    24181 agtaaagtaa aggaaagtaa agtaaataaa ataaaagtaa atgaaagtaa agaaaacaca
    24241 aatagtgaag aaaatcaact tgtgtttttt gaaaatacgg acattcattt ttcgattctt
    24301 gaatttttcg gatttaccga aatgcgaaac ccggataaac ttcagcagat ttcaattttc
    24361 ctgaatatac ttttcaccga cgggcgaatt aatcagttta ccgatcagtt tgaagcttat
    24421 cgcctgtata aggaaaaatc gagcacggcc aaacattcgt ttccgcgatt tttgggaacg
    24481 attgaaacca gatatcttga tggtggttgg aatcaagaaa actggaccga ccgcctgaat
    24541 gcaattgcaa aacgagaatc atccgattcg ccgtttaact cagaaaaaac gcaaagcctg
    24601 cgggaaagga tcgtaaatgg aaaagactga tttttcgcga accgaaatta aagacattag
    24661 ccgaacggat attgccgggc tagttttcga tatagttgaa gatttgcaca aaatgtgctc
    24721 gcaaaaaatt gacgatgata atgttcagtt tactgttaaa aagtttgttg agttactgaa
    24781 tggtccatat cgttcctggt gttggggcga agtgcgaagc gttatgaatt tgggtgtaac
    24841 agggtcgtat ggaggcaagt cagaaaaaat aaactttcag acgcttgcga catggatgaa
    24901 gcgatctcag gctggtcgct cgtcgaacta tgccgaaagg tcgatttacg aggcgcacca
    24961 aatagctggt gaaacagtag gaaattttgc ggatatatcg gacaggtgga aagaatttag
    25021 agaatggatg caggaaaatc acctttggtt tgtagataac atttccgaag aaaactgcaa
    25081 tctgtatcat tctgcaaaac gaacccgcag tttggaaaat ttcaaagata tgcttgctat
    25141 tgagcccgat ccgggctata tggaaagggt aaaaaaaact acacttgaac aattcagaat
    25201 agagcatgaa atacttgatt gattttgata ttcgattatc agaagacaaa aaaacgcttg
    25261 aattgctgag aaataaccaa gtttttgaaa aaattgaagg cgaacaggct aacgaaattt
    25321 acaagaagtt ggtaaaaatt gacgatttaa aattactcgg attttcatac tgttatcttg
    25381 aatttgacgg aaaactagac gaatggtacg aattgcgttt ggttgaacaa aatgctttaa
    25441 cagaaggtgt ttcgctgatg tatcatccta aaactgattt gtttgaagtt cctgaaaacg
    25501 gattggtttt ttctaaatat gaaaaagtgg aaaaatacat aaaagcattt tcgttatgaa
    25561 aaattacaat cacgaaatat ggcacatgtg taagtattgc ggctgcgagt tcgatatgcg
    25621 attatgtaat tgctactgcc caaaatgcgg aaagaaaaat aacatttaac ttattaaatt
    25681 atgaaatgga ttttgcaacc gataaaatct ttccgggaat ggaaagaacg ccgatacctg
    25741 aaacgtatga ttactgaagc gaaacgtttg catc
//
LOCUS       DAOPZK010000012        25035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_1980_length_25035_cov_20.153723, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000012 DAOPZK010000000
VERSION     DAOPZK010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25035
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1980_length_25035_cov_20.153723"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1371
                     /locus_tag="PKH68_01480"
     CDS             154..1371
                     /locus_tag="PKH68_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNX11634.1"
                     /translation="MKTYLIAILALVAFAGCTVGNPVEDLEAKKIELTAVQEQRVFQD
                     NDFAFELLRKTIANTEDKNVFMSPLSVSLALGMVMNGANGTTLTEIRQVLGMNGMNST
                     DVNEYYELMQKTLPAIDLKTKLSLANSIWYRNGFNVKPSFLKINSDYFNATSQGLDFA
                     SPAALETINGWCSDNTNKLIPKVLDQIDGSTVMFLLNAIYFKGNWVTKFNKDKTFTGE
                     FVNDEGKNTEVDMMQLKSEFGYYEDDVAEYLCMPYGNKAFSMIAILPDEGKSTDDVLR
                     VLTAEKFSDAVKKMSTEEVQVYFPRFKVKNKFQLKPMLQAMGMLQAFEPSADFGGISD
                     NRPLWIDYVQHDTYVDVNEEGTDAAAVTTVAFKESAGLMFRADKPFLFMIREQSTGII
                     LFVGKISNPGKYS"
     gene            1386..2090
                     /locus_tag="PKH68_01485"
     CDS             1386..2090
                     /locus_tag="PKH68_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11635.1"
                     /translation="MKTVNYIFASIILSFLPFLGGCHNDTDLSEGYLTAVITGYNPTC
                     GACILTFTDNDKEVERLLGISEKNNYLAVNLNGDLSTQPGRPIKVKVRKATGNEIPAC
                     SGNYPAVFVIDYISITFSYEQEFEIPVKGSVLSKDEKYLLRFDSVLTDSRCPEGVYCF
                     WQGMAGVRLTITQNHFAPETLDLYTWNSERMNYCDSAEYKNLIIRLLELNPYPSTKVR
                     YGYDSYKAKILITLKK"
     gene            2097..3365
                     /locus_tag="PKH68_01490"
     CDS             2097..3365
                     /locus_tag="PKH68_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11636.1"
                     /translation="MSKLKFMFLFAGVFLLAACSEYVEETYTYKINEPVFMAVTEFRN
                     SVKVSRIPQEIHKQGKICFYKDYLYISEPDKGIHIVNNVDPANPKVVGFIEILGNADI
                     TVLNDVLYADSYIDLVWFDISNPAAPVPEGRKENVFPTAIPPMDNFYGYDYEQCSDKT
                     KGVIVGWEVKEKSGTYNKSRWWWWRGGMTNDLLMAESYAKSGSNTGITGSMARFSIYK
                     NYLFTVLNNYMGVFDLSQRTPVVVADSIYVGWDVETIFSYKDYMFLGTPRGMLIYNVT
                     NPVKPVYLSSFQHFYGCDPVVVENDKAFVTVRSGNMCGQNSNELIIVDVSNVKAPKQL
                     ASYAMTSPKGLGIDNGTLFLCDDGLKVYDASDPMVMMGKQLAHYKGMDGYDVIPYDKT
                     LMMIAGDGIYQYDYTDLNNIKPLSKLPIGQ"
     gene            complement(3532..4812)
                     /locus_tag="PKH68_01495"
     CDS             complement(3532..4812)
                     /locus_tag="PKH68_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNX11637.1"
                     /translation="MKNICFYFEIHQPLRLKRYRFFDIGQDHYYYDDFQAEERIRGMV
                     EQSYLPANRTIAEMIRSSNGKFKCAFSISGVALEQLEQYAPEVIDSFQELAKTGSVEF
                     LAEPYAHSLASLYNTDEFVEQVNLHADKIEQLFGKRPTAVHNSELIYSDEIGEVLQKM
                     GYKTVLIEQVKDVLGWKSPNYVYHHPSNPKLKLLVRNNKLSDDIAFRFSNRAWADFPL
                     TSEKFVSRIAEASDGEQIFNVWMGYEALGFYQHPESGIFEFLKAVPFHAIEHSIGFVL
                     PSEATKKLEAVDSLSFTHPTSWAESKDISVWNGNDLQHEALSKLYAVSERVHLCNDKP
                     LQHDWLLLQTTDNFRYMSHSDSFGTHYVSPYEAFTNYMNVLADFLDRVDAQYPTTIEN
                     EELNALLKTINNQEKEIAELEEEIKKLKSRKEKK"
     gene            complement(4856..6115)
                     /locus_tag="PKH68_01500"
     CDS             complement(4856..6115)
                     /locus_tag="PKH68_01500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNX11638.1"
                     /translation="MKALMFGWEFPPHILGGLGTASYGLTKGMSLQQDMEITFVIPKP
                     HGDEDQSFLKIIGACNVPVVWKDNTWEHVNNRIGKIMHPDEYFWLRNGIKYDFTRIFT
                     NDLGCIDFSGRYPDNLIEEISNYEAVASVLAHQLEFDVIHSHDWLTYPAGVFVKRITG
                     KPLVIHVHATDFDRSRGKVNPTVYNIEKTGMDIADHIITVSNLTRKIVIEKYHQDPRK
                     VTTVHNAVEPLEHVESFVKKPRKDKVVTFLGRITMQKGPEYFVEAAHRVIQRTKNVRF
                     VMAGSGDMMDAMIDLAAKRGIADRFHFPGFQRGSEVHEMLAQSDVYIMPSVSEPFGIS
                     PLEAMQVGTPSIISYQSGCAEILTHVIKTDYWDIDAMADAVYSIVTNPSMYKSLRELG
                     LEEVNNIKWYDAGLKVRNIYNLVTGMH"
     gene            complement(6112..8061)
                     /locus_tag="PKH68_01505"
     CDS             complement(6112..8061)
                     /locus_tag="PKH68_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNX11639.1"
                     /translation="MSYLNFDKTLLMNLEKSISKEMIRTNRAGTYNFTTLIDCNTRKY
                     HGQLVMPLPDIDNSNHVLLSSLDETVIQHGAEFNLGVHRYEGNNYFPNGHKYIREFDC
                     ELISRTIYRVGGVILSKERMLVSFEPRILIKYTLLEAHSPTLLRFKPFLAFRSVNELT
                     HQNNIANTSYSELQNGISMKMYEGYPNLCMQFSKTNTYVHQPDWNKNIEYYKEQERGY
                     AFKEDLLVPGYFEMPIKKGESIIFSGGVTEVSPRTLKNLWDKELTRRIQRSDMFSTLK
                     NSAEQFYKRVGDKTYLMAGYPWFGARARDEFVSLVSSTILIDRMDYFDAIIDTAIPEI
                     ERFMEGEREGFVLEGIDDPDVFLYFILAVQQFSTHRTMEEAASRFGKLVAKIIHYIRK
                     QNHPNLIMHNNGLLYVNGTDKPATWMNAVENGRPITPRTGYVVEINALWYNALKFASL
                     LENYSGNEQSSDLLNYQAEIARDAFVRIFWNGTYLYDYVADDYNDEEVRPNMIMAVAL
                     PFSPLDKIQRKSILDITTRELLTPKGLRSLSPKSGSYRPEYIGGQKERDWNYHNGPVW
                     AFTFGAFASAYLRIYKKSGKSFIERMLNGFEAEMTELCVGSLSELFDGNPPFKGHGAP
                     SFAMSVAEILRVLDMLKKYDNETTV"
     gene            complement(8257..9846)
                     /locus_tag="PKH68_01510"
     CDS             complement(8257..9846)
                     /locus_tag="PKH68_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX11640.1"
                     /translation="MKTKKITYLAALMVSLILTFTACNLDLGPIDNYGNANFWKTLPQ
                     AQAYVNGMHMDLRSTSFNRNYILGEARGGLLVTGNSSQGIAPDYGDIKKNDVNGNTPG
                     VSGWVDFYGKIFDFNLMIQNIENSDIHKNNQKDVDYLLAQTYGMRAFYYFTLYRTFGG
                     VPLVTRVKVLDGQVNAVDLYTARANPKETMDFIKSDIQKSLDYFVSAGKEWTNNVTWS
                     KAATQMLAAQTYLWSAKVTLGNQTPDAGDLAKAESYLNDIKNNTRFGLLSDFSKIFTS
                     DNEGNKEIIMAIYYADGEATSNVGNFLYAAAQNTFYTKTCTKVVDPLKILSTGIQRNE
                     YTLDFYKSYSDKDTRRSATFMDYYTPSSALMGTVMKKFIGSINSTGARVYDCNEPIYR
                     YADVLLMLAEVENMKGGDPAQYINQIRQRAYGADYVAATDAYVNADFKTNEYAILKER
                     DKEFVYEGSRWFDILRLKDGINGQPLAFDAASAYTTTPVLKTTEKYKVLWPIDKATLN
                     ADPLLKQTPGYKVADQVEEVW"
     gene            complement(9870..13253)
                     /locus_tag="PKH68_01515"
     CDS             complement(9870..13253)
                     /locus_tag="PKH68_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX11641.1"
                     /translation="MKKKLILICMLLLTLAATAQKKAVTGTVTDTNKEPLIGATVREA
                     GTTNGTLTNFDGNFTLNVADGATLEVSYVGYTSAKIKASFGSPNLVSLKDDAALLDEV
                     VITGYTGTQVRSRSTNSIAKVDNKKLTVGVFSNPGQALSGAVSGLRVTQTSGNPGAAP
                     TITLRGGTNLDGSGSPLVIVDGQIRGSLSDINPEDIEDMQVMKDAGATAIYGARANNG
                     VILITTKKGKSGQSEINFKAKVGVNNLINPYKFTDAETYLTWQRNAYLNSHNVWTKAD
                     GTPVGFSPIANLTKNTPMGTGNSYFASDGKTILNDGNSIWSTMYLNDQNKILLDQGWQ
                     KMRDPAYDFMLANYQKPWYKSQFGTTFVPQEYLIFKNTNPADYNFKDPSYTQDYNVNM
                     SGGNDKGHYYSGIGYNYSQGLPINSYYKRYSFIFNGDYKIKPWLTSISSINYGRANWQ
                     SMPGSQGDEANYFSRILSVPPTVRFSNEDGTPRLGNNAGDGNQNFQPNQWFIFNQTDK
                     FTMNQAFKVDFTKSLYLRMAANWYYSEGNYESFTKDYYNTPTQISTNRSTSAEFNRSF
                     EQTYNAVLNYEKQINSHYLAVMLGTEFYDSFYNGFSASGSGAATDDFRDLSLTSSDKD
                     KRSIDSYHTQQRILSFFGRVNYDYMGKYILSAVVRRDGYSRLIDNRWGTFPGFSAGWM
                     FSKESFMNPLKDVISFGKLKTSYGLNGNVSGIGAYELQGGYSGSTKYNGQSTFILSSV
                     PNPGLIWEKTRTFEVGLDLSYFENRYSTNFTLYDRLTSDKFASLSLPSSSGISSIRTN
                     NGQFRNRGLEIELAAKPLQSKNWNWSINANIAYNKNTVVKLPNNGLPLNRQQAFQVYS
                     GKDATDLLWVGGYQEGQEPGVLYVWQADGIYKNESEIPDNMKVLSYNYVGAFGGPSKT
                     LWGKAQWAAMSETDRGTSNYPIQPGDVKWKDINGDNIIDEYDKVKVGNTTPHWIGGIN
                     STLSWKNLSLYTAFDYALGFWMMDYRLPWLMGNMQGTYAMPMEVTNTWTSENPDAKYP
                     RYTYADQLGKGNYLRSSTLFTNRGDYLSFRQISLTYSVPREIINKGFIQKLDISVTGQ
                     NLGYLTAGKTFANPEKGGGIVDAGYSLPRTVTFGLNISF"
     gene            13734..14243
                     /locus_tag="PKH68_01520"
     CDS             13734..14243
                     /locus_tag="PKH68_01520"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX11642.1"
                     /translation="MPFIHSDLSHLLNNIISFFVLSSCLYYFYSQIANKILLLSVVFS
                     GLILWVIGRESWHIGASGVIFAISFFLFFSGLLRKHIPLIAISFVVVFLYGNNVWHLF
                     PWSKIDPVSWEGHLAGGITGVLLAVIYRRQGPQKPVKEWEDDDENEDDEAEYQDNDPE
                     LNKENLSEN"
     gene            14264..15229
                     /locus_tag="PKH68_01525"
     CDS             14264..15229
                     /locus_tag="PKH68_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11643.1"
                     /translation="MENSKPNEVNLLDILVVSGKWIKNLFKGLLRFAGYILKLLYRNK
                     ILTAIIFLLAIVIGQYMARPSARKFNVDAMCVLYGPEATTISEVGKQLSLSSPRYEAT
                     SLANKLSLPDSIASKVAGVEFFQVIDYQLDHTPDEIDFSNSHSLEDTLNLPMRDYIYM
                     RIKVKGTRHAQAIGEAMLKYFNTNPTIQNQYNIFKAQLNEKVNLCDAEMLRLDSLAKI
                     TYFRDKKPEIKFESNKLLIGNNEVQLFYNDLLNLQANKAQAMAQLEVAKQPVVIPSGF
                     VINPNSVNGRVKLGVYSLFIGLIISTILGYYIENRKKWLKYLQEK"
     gene            complement(15283..17871)
                     /gene="clpB"
                     /locus_tag="PKH68_01530"
     CDS             complement(15283..17871)
                     /gene="clpB"
                     /locus_tag="PKH68_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNX11644.1"
                     /translation="MNFSNFTTKSQEVVQHAIDLAQANNQQAIETAHLMKALLSKGED
                     VIPFLFAKMGIYTSNIQQVIDKNLESFPRVTGGEPYLSRETNEVLRKATEISTVAGDQ
                     FVSLEYILLALLKVKNTVSQLLKDAGVTEKALESAIAELRKGSKANSATAEETYNSLE
                     KYAVNLNERARSGKLDPVIGRDEEIRRVLQVLSRRTKNNPILIGEPGTGKTAIAEGLA
                     QRIVRGDVPENLKTKQVFSLDMGALIAGAKYKGEFEERLKSVVNEVIKSDGEIILFID
                     EIHTLVGAGKSEGAMDAANILKPALARGELRAVGATTLNEYQKYFEKDKALERRFQIV
                     MVDEPNIASSISILRGLKEKYENHHKVRIKDDAIIAAVELSNRYISDRFLPDKAIDLM
                     DEAAAKLRMEVNSVPEELDKIERNIKQLEIEREAIKRENDVRKLEQLQEEIANLKEES
                     KKYRAKWSSEKELIEDIQQSKIEIENLKYEADRAEREGDYGRVAEIRYGKIKAEENRI
                     AENQSRLVEIQGNNAMIKEEVDSQDIADVVSRWTGIPVTKMLQGEKEKLLHLEEELHR
                     RVVGQDEAIAAVSDAVRRNRAGLNDPKRPIGSFIFLGTTGVGKTELAKALAEYLFDDE
                     NMMTRIDMSEYQEKFSVTRLIGSPPGYVGYEEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNTLLQVLDDGRLTDNKGRTVNFKNTIIIMTSNIGSTLIRENFEQINNQNRNEIIE
                     KTKNEVFELLKKAIRPEFLNRIEEIIMFAPLDESQITEIVKMQLNGIKNMLAENGVKL
                     EITEKALEFIAAEGYDPQFGARPVKRAIQRYVLNDLSKELISGKVNNEQPIIVDCING
                     KIIFSN"
     gene            18133..18777
                     /locus_tag="PKH68_01535"
     CDS             18133..18777
                     /locus_tag="PKH68_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNX11645.1"
                     /translation="MQKIDFNVPEPTLRRLPWYLAYTKLVQQAGESYLSSTQIASNIA
                     VDASKVAKDLSYVQISGRTRVGYDVNELVNVLETFLGFTNSHKAFLFGVGSLGGALLH
                     DDGLNQFGLEIVAGFDVKYELAGTSIHRVPIHHIDHFIELQKQTKINIGVLTVPVDKA
                     QETAEMMIDGGIQAIWNFTPFRIRVPENIIVQNTSIYAHLAVMFNRLNELNSKR"
     gene            18783..19394
                     /locus_tag="PKH68_01540"
     CDS             18783..19394
                     /locus_tag="PKH68_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNX11646.1"
                     /translation="MKIFAIGQNYAEHNKELNSPDPTEPVIFMKPDTAALKNNKPFYI
                     PDFSSELHYETEIIVKISRLGKNIAPKFAHRYYEEIGLGVDFTARDVQRRLKAEGKPW
                     EISKAFDNSAVIGNFIPKEELGDVQNIHFHLNINGNTVQKGHTSDMIFPVDEIIAWIS
                     RFFTLKIGDILFTGTPVGVGKVNIGDHLEGYIGEKKMFDFKVK"
     gene            19710..23504
                     /gene="porU"
                     /locus_tag="PKH68_01545"
     CDS             19710..23504
                     /gene="porU"
                     /locus_tag="PKH68_01545"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNX11647.1"
                     /translation="MKYNILTFLLLMISVATLSSQNISQTKHQIRWTGIETWQTDKAQ
                     KKVLSFTDASYPAENDLPYFVQTISWDKNYDVDARIENPVYTSLTSEEIKLLDNINLS
                     ETPEISTYITEERGNSYLNIQILPLIKLEGKVKKLTSFELNVTKTSKSFKAPAAIHSY
                     VSSSVLATGKFVKIKIKDSGIYKLTYEALNSMGVNPANVRIFGYGGALLEQSFLQPKP
                     DDLPEVAVWMEKGSDGVFNAGDYILFYGQGIVKWKYDPAKAMFVHTLNHYSNDAFYFV
                     TSDAGTGKKIADQTIDVPADAIVADVNEFTDYQLYELEKQNLAQTGKVFYGETFSDVV
                     SYNFPFTFTNMVKTASTKVRIDVAAISSENSTFTLKLNDDQPQNVTVIKKSVDNYEIG
                     KDANAVLNFTPSADNLIFNLTYNKPTTVSRGFLNYLEINARRSLTMIGAVMFFRNTDN
                     LNTATYNRYQLSGAGPNVQIWDITDRLNIKKVITTRSNNVLEFKDTNSSLKEYVAIDP
                     TAASAFQQPTTVGAVPNQNLHALAPTEMVILTHPSFVVQAEKLAQAHRVKDDMTVAVV
                     TTEQVYNEFSSGTPDATSYRWVMKMLYDRAIAAGNTSAAPKYLLLFGRGSFDNRGIIL
                     NSGNNLILTYQADMSLDVVKSYVTDDYFGFLDDNEGLQIASHLLDVGIGRFPVVTPQQ
                     ADDVVNKTISYMENKENGNWKNQLCFLADDGDGALHMRQADSIASLVSRSNPGFQYNK
                     IYLDAYLQEVAASGESYPLAHTRFHNMLRSGIFMLNYTGHASQLGWTNEQILTTADVK
                     QMYNTKLPFWIGATCDFILFDVKDISAGEHVVLNPAGGGIGLFSAARTVYASQNEKLN
                     RYFTVNLFTKPNGKYYRLGDVMARAKNQVGTEINKLSYTLLGDPALRLNYPTDYNVVT
                     EKINNRTVQAADTLRALSVNSIEGYIADGSGNRISEFNGNLEVTIYDKMQKITTLNNH
                     NDGTLTYNDRPNILYSGKAKIQNGNFSFTFMVPRDIKYNYGSGRISYYASDSLKHKEA
                     QGYYENFIVGGGTNIDLNADQAGPELKVYLNSENFVSGGKVNESPLFFARISDVNGVN
                     TVGSGIGHDLRLVVDDNQFTSYTLNDYFEAETNSYKSGSVRYKLPLLPAGKHTLTFHA
                     WDLLNNSSQATLDFEVVVGLTPTIFKVYNFPNPVRNETRFVVEHDRPETILETTVDLY
                     DMTGRKIWSMKKNSVENLKWNRADASLQQVKPGIYIYKISVKTANSEMTSKANKIIVT
                     GQ"
     gene            23662..24849
                     /gene="porV"
                     /locus_tag="PKH68_01550"
     CDS             23662..24849
                     /gene="porV"
                     /locus_tag="PKH68_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNX11648.1"
                     /translation="MKKTYLILFSLLSLSLTSFSQTTVNDQLNPLITGVPSLTITPDA
                     RGGGMGDVGVATSPDINSQYWNPAKYAFMTSPAGLSFSFTPWLRSLGINDINLSYLTG
                     YWKFDDLQSVSGSLRYFSLGSVRLTDEQGNDLNMDAQPNELALDVAYSRLLSDNWSAA
                     VGFRYIRSDLNNGANGAGGIEMFPGNAFAADIATYYKTPIAMATGDANLAFGLNISNI
                     GSKISYDQNETSIFIPTNLRLGGSFEYPFDDYNKISFNADINKLLVPTPVKPIGEETY
                     ESQSYLDRKAIYDQTGPIAGIFKSFNDAPGGMSEEMKEIMWSAGAEYAYNNQFFVRGG
                     YFNEHAMKGNRKYFTAGAGFKLNVFQLDVAYLISVAQSNPLDQTLRFTLGFDLNGLTE
                     LMR"
     gene            24944..>25035
                     /locus_tag="PKH68_01555"
     CDS             24944..>25035
                     /locus_tag="PKH68_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNX11649.1"
                     /translation="MKIRVGFGYDVHAFAPDRELWLGGVKIEHTV"
BASE COUNT         7044 a         5201 c         5313 g         7477 t
ORIGIN      
        1 atattttgtt gccgaccgcc cgttcctttt tgctatccgg gagaaaagca ccggcataat
       61 cctctttgtc ggcaaaatgg gaaacgtgga aaaattctga tgtaaagaag gtgtaatttt
      121 atttttaacc aataaatttt taaccaacag attatgaaga cttatcttat tgcaatactg
      181 gcacttgttg catttgcagg atgtacagtc ggcaatcctg tggaagattt ggaagccaaa
      241 aagattgaac ttacggctgt acaggagcag cgggtatttc aggacaatga ttttgcattc
      301 gaattactcc gtaaaacgat agcaaatacg gaggataaaa atgtgtttat gtcaccgctg
      361 agtgtaagtc tggcactggg catggtgatg aacggagcca acggaaccac actgacagaa
      421 atacggcagg tactcggcat gaacgggatg aattccaccg atgtcaatga atactatgag
      481 ctgatgcaga aaaccttacc tgccattgac ctgaaaacaa aactgagttt ggccaattcc
      541 atttggtacc gaaatgggtt caatgtaaag ccttcgtttc tgaaaattaa ctccgactac
      601 ttcaatgcga cttctcaggg acttgatttt gcaagccctg cagctctcga aaccatcaac
      661 ggttggtgca gcgacaatac caataagctg atacccaagg tgctcgacca aatcgatgga
      721 agcacggtta tgtttttgct caatgccatt tatttcaagg ggaattgggt gaccaaattc
      781 aacaaagata aaaccttcac cggtgaattt gtgaatgatg agggaaagaa cacggaggtg
      841 gatatgatgc aattgaagtc ggaattcgga tattatgaag atgacgtggc tgaatacctt
      901 tgtatgcctt atgggaataa agctttcagc atgatagcca tattacccga cgaaggaaaa
      961 tcaacggatg acgtgctccg ggtgctgaca gctgaaaaat tttcggatgc tgtgaaaaag
     1021 atgtcaacag aggaagttca ggtgtacttc cccaggttta aggttaaaaa caaatttcaa
     1081 ctgaaaccca tgctgcaggc aatgggaatg cttcaggcat ttgagccgtc ggccgacttt
     1141 ggaggaatat ctgataacag accgctttgg attgattatg tgcagcacga cacgtatgtg
     1201 gatgtgaatg aagaaggaac cgatgctgca gcagttacca cggtcgcatt taaggaatcg
     1261 gccgggttaa tgttcagagc tgataaaccg ttccttttta tgatacggga acaaagtacg
     1321 ggaattatcc tgtttgtggg aaaaatcagc aatccgggta aatactccta aaaagctgta
     1381 aaaagatgaa aactgttaat tacatattcg cttcgataat cctctctttc ctcccgtttt
     1441 taggtggttg tcacaacgat accgatttgt cggaaggata tctcaccgct gtaattaccg
     1501 gttacaatcc cacttgcgga gcatgcattc ttacgtttac ggataatgat aaggaggtgg
     1561 aacgactgct gggcattagc gaaaaaaata actacctggc tgtgaatttg aatggagacc
     1621 tgagtacaca gcccggaagg ccgataaaag tgaaagtccg caaagccacc ggcaatgaaa
     1681 ttccggcttg cagcggcaat tatcctgctg tatttgtaat cgattatatt tctattacct
     1741 tcagctatga acaggagttt gaaattccgg taaaaggatc ggtgctcagc aaggacgaaa
     1801 aatatttgct ccgatttgat tcagtattga ctgattcacg atgtccggaa ggcgtttatt
     1861 gcttctggca gggaatggcg ggggtacgtc ttaccattac ccaaaatcat tttgctcctg
     1921 aaactctgga tctgtatacc tggaacagtg agcggatgaa ctattgtgat tcagccgaat
     1981 ataaaaatct gatcatcagg ttactcgaat taaatcccta tccctcaacc aaagttcgtt
     2041 acggctatga cagttacaaa gctaaaatac taataaccct gaaaaaatag aatattatga
     2101 gcaagttaaa atttatgttt ctctttgccg gtgtgtttct gctcgctgcc tgttcggaat
     2161 acgtggaaga aacctatacc tacaaaatca acgaaccggt atttatggct gtcaccgagt
     2221 tccgcaactc ggtaaaggta tcgcggatac cacaggaaat ccataagcag ggaaaaatat
     2281 gtttctacaa ggattatcta tacatctccg agcccgataa aggtattcat attgtgaaca
     2341 acgttgatcc tgccaatccc aaagtggtcg gatttatcga aatcctcggg aatgccgata
     2401 ttaccgtgtt gaacgatgtg ctctatgctg attcgtacat cgacctggtt tggttcgata
     2461 tctccaatcc ggctgcaccg gtacccgaag ggcgtaagga aaatgtattc cccacagcca
     2521 ttccgcctat ggataacttc tacggctatg attacgaaca atgctccgac aaaacaaaag
     2581 gagttattgt gggctgggaa gtgaaggaaa aaagcggaac gtataataaa agtcgctggt
     2641 ggtggtggcg tggaggcatg accaacgacc tgctgatggc cgaaagttat gctaaaagcg
     2701 gcagcaatac cggaattacg ggctccatgg cacgattttc aatttataag aattacctgt
     2761 ttacggtgtt gaacaattat atgggagttt ttgatttgag ccagcgcacc cctgtggtag
     2821 tggccgacag catctacgtg ggatgggatg tggaaacaat tttcagctac aaggattata
     2881 tgtttttggg tacgccacgc ggcatgttga tttataatgt caccaatccc gtgaaaccgg
     2941 tctatctttc ttcgttccag catttctacg gctgcgatcc ggtggtggtc gaaaacgaca
     3001 aggcatttgt gaccgtgcgt tccggcaata tgtgcggaca gaattcgaac gaactcatca
     3061 ttgtggacgt gagcaacgtg aaagccccca aacaactggc ttcctatgcc atgacaagtc
     3121 ccaaagggct gggcattgac aacggtactc tctttttgtg tgacgatggg ctgaaagtgt
     3181 atgatgccag cgacccgatg gttatgatgg gcaagcagtt ggcacattac aagggtatgg
     3241 acggatacga tgtgataccg tacgataaaa ccctgatgat gatagccggt gacggcattt
     3301 accagtatga ttacaccgat ttgaataata ttaaaccatt gagcaaactg cccattggac
     3361 aataaacatt tactttctgc aacttcatca tcccgccgca gtgatgctcc ggggtgattt
     3421 tttttgtaga taatcttccc ttgataatct acactgctca atcgcaaaaa taaccccgcc
     3481 gatatacaca gcggggttat ttatgtttat cctattgata tgaactgaca attatttctt
     3541 ttctttcctc gatttcagct ttttgatttc ctcttccagc tctgctattt ctttttcctg
     3601 gttattgatg gttttaagca gggcattgag ttcttcattc tcgatggtgg tgggatactg
     3661 cgcatccact ctgtccagaa aatcggccag cacattcatg taattggtaa atgcttcgta
     3721 gggcgaaaca tagtgtgtgc caaatgaatc ggaatgagac atgtaccgga aattgtcggt
     3781 ggtctgtagc agcaaccagt cgtgttgcag gggcttgtcg ttgcacagat gcacccgttc
     3841 gcttaccgca tatagtttgc tgagcgcttc gtgctgcagg tcgttaccat tccatacact
     3901 gatatccttg ctttcggccc agctggtagg atgagtgaac gagagtgagt cgactgcttc
     3961 cagttttttg gtagcttccg aaggcagcac aaaaccaata ctgtgttcga tggcgtgaaa
     4021 aggaacagct ttgaggaatt cgaaaattcc ggattcggga tgctgataaa aaccgagtgc
     4081 ttcgtaaccc atccacacat tgaaaatctg ctcgccatcg gatgcttcgg ctatcctgct
     4141 cacaaatttt tcggaagtga gcggaaaatc agcccaggcg cgattggaaa agcggaaagc
     4201 aatgtcgtcg ctcagtttat tgttgcgcac caacagtttc aattttggat tggacgggtg
     4261 gtggtaaacg taattcggac ttttccatcc caacacatct tttacctgct cgatgagtac
     4321 cgttttatat cccattttct gcaatacctc accaatttca tcggaataaa tgagttcgga
     4381 attgtgtact gcagtaggtc ttttaccgaa aagctgttcg attttatcgg catgcagatt
     4441 tacctgttct acaaattcgt cggtattgta gagcgaagcc agtgagtgcg catagggttc
     4501 agccagaaat tccacgctgc ctgtttttgc cagttcctgg aaactgtcga ttacctcggg
     4561 tgcatactgt tcgagctgtt cgagtgccac tcccgaaatg gagaatgcgc atttgaattt
     4621 tccgtttgaa ctcctgatca tttcagctat ggttcggttg gcaggcagat acgattgttc
     4681 aaccatgccg cgaatgcgct cttcagcctg aaaatcatcg taataataat gatcctggcc
     4741 aatatcgaaa aaacggtatc gtttcaggcg cagtggctgg tgtatttcaa aataaaagca
     4801 aatgtttttc atatcgttcg ttttttatta ttttttcctt gggtaatact tgtttttaat
     4861 gcattccggt aaccaggttg taaatgttcc tcactttgag tccggcatcg taccacttaa
     4921 tgttgttgac ttcttccagt cccagttcac gcagactttt atacatcgat ggattggtca
     4981 caatgctgta aacagcatca gccatagcat caatatccca atagtcggtt tttatcacgt
     5041 gagtgagtat ctcggcgcat cccgactgat aggagattat ggacggggta cctacctgca
     5101 tggcttcgag aggcgaaata ccgaatggtt cggaaaccga tggcataata tatacatcgc
     5161 tttgcgcaag catttcatga acctcactgc cgcgttggaa acccggaaaa tgaaagcgat
     5221 cggcaattcc ccgcttggcg gccaggtcta tcatagcgtc catcatatcg ccgctgccag
     5281 ccattacaaa acgcacattt tttgtacgtt gtatcacccg gtgagccgct tccacaaagt
     5341 attcgggtcc tttttgcatg gtaatacgtc ccaggaaggt gacaacctta tccttacgcg
     5401 gtttttttac aaaactttcc acgtgttcga gtggctcaac agcattgtgc accgtggtta
     5461 ccttacgcgg gtcctgatga tatttttcaa tcactatttt ccgggtcagg ttgctcactg
     5521 taatgatatg gtcggctatg tccataccgg ttttttcgat attgtacaca gtcggattta
     5581 cttttccgcg gctacgatca aagtctgtag catgaacatg aatgaccagt ggttttccgg
     5641 taatccgttt tacaaataca ccggcagggt aggtgagcca gtcgtgtgag tggatcacat
     5701 caaattcgag ctgatgagcc agtacactcg ctactgcctc gtaattggat atctcttcaa
     5761 tcagattgtc gggatagcga cctgaaaagt caatacaacc aagatcgttg gtaaaaatgc
     5821 gtgtaaagtc gtatttaatg ccattgcgaa gccagaaata ttcatcggga tgcattatct
     5881 tgccgattcg gttattgaca tgctcccacg tattgtcttt ccaaacaaca ggaacgttgc
     5941 aggcaccgat gattttcaga aaactctgat cttcgtcgcc gtgaggtttg gggataacaa
     6001 aggtgatttc catgtcctgt tggagcgaca tccctttggt aagtccgtaa cttgcagtgc
     6061 ccaaaccgcc taaaatatgg ggaggaaact cccatccaaa cataagtgct ttcataccgt
     6121 tgtttcgtta tcgtattttt tcaacatatc caatactctc aatatttcgg ctacactcat
     6181 ggcaaacgaa ggtgctccgt gccctttgaa cggtggattt ccatcgaaca attcggatag
     6241 tgaacccacg caaagttcgg tcatttcggc ttcaaatcca ttgagcatac gttcaataaa
     6301 agattttcca ctcttcttgt aaattctcag atatgcactg gcaaaagctc cgaatgtgaa
     6361 tgcccaaacc ggaccgttgt ggtagttcca gtccctttcc ttttgtccgc cgatatattc
     6421 cgggcggtac gaaccgcttt tggggctgag tgaacgcaat cccttgggtg tgagcaattc
     6481 acgtgtggtg atatccagga tggattttcg ttgtattttg tcgagcgggg agaagggcag
     6541 cgccactgcc attatcatat tgggccgaac ttcttcatcg ttgtagtcat cagccacata
     6601 atcgtagaga taagttccgt tccagaatat tcttacaaat gcatcccggg ctatttctgc
     6661 ctgatagttg agcaagtccg atgattgttc gttgccgctg taattttcga gcagggaagc
     6721 aaatttcagt gcattgtacc acaaggcatt gatttcgact acgtagccgg tgcgcggagt
     6781 gatgggtcgt ccgttttcca cggcattcat ccaggtggcg ggtttatcgg tgccgttgac
     6841 atagagcaac ccgttgttgt gcatgatcag gttgggatgg ttctgtttgc ggatgtaatg
     6901 gataattttg gcaaccagtt ttccaaaacg gcttgccgcc tcttccatgg tcctgtgtgt
     6961 ggaaaattgc tggacggcca gtatgaaata gaggaaaaca tcgggatcat caatgccttc
     7021 gagcacgaag ccctcgcgtt cgccttccat aaaccgttcg atttcaggta ttgctgtgtc
     7081 aatgattgca tcgaaataat ccatccggtc gatgaggatg gtactggata ccagtgacac
     7141 aaattcatcg cgtgcccgtg ctccgaacca gggatatccg gccatgaggt aagttttatc
     7201 gcctacgcgt ttgtagaatt gttcggctga atttttcagg gtagaaaaca tgtcggaacg
     7261 ttgaatgcgg cgggtaagtt ccttatccca gaggttttta agtgttcggg gcgaaacttc
     7321 ggtaacacct cccgagaaga tgattgattc gcctttcttt atcggcattt caaagtatcc
     7381 aggtaccaac aaatcctcct taaaggcata tccgcgttcc tgttccttgt agtattcaat
     7441 gtttttattc cagtcgggct gatgcacgta ggtattggtt tttgagaact gcatgcaaag
     7501 gtttggatat ccctcgtaca tcttcatgct gatgccattc tgcaattcgg agtacgaggt
     7561 attggcgatg ttattctggt gagtaagttc gttcacactc cggaatgcca gaaaaggctt
     7621 gaatcgcagc agggtgggtg agtgcgcttc aagcagggta tatttaatca gaatccgcgg
     7681 ttcgaacgat acgagcattc tttctttgga tagaataaca cctcctacgc ggtaaatggt
     7741 gcgggaaatc aattcgcaat caaattcccg aatgtactta tgtccgttgg ggaagtagtt
     7801 gttaccttca tacctgtgaa ctccaaggtt aaattcagca ccgtgctgta tcaccgtttc
     7861 gtcgagcgag gaaagtagta catggttcga gttgtcgata tcgggaagag gcatcaccag
     7921 ctgaccatga tattttcggg tattacagtc gatcagggtt gtaaaattat acgttcctgc
     7981 ccggttggtt ctgatcatct cttttgagat tgatttctcg agattcatca gaagcgtttt
     8041 gtcaaagttt aaatagctca tttttcaaaa gataatgttt gttaaggctt tcacaaaaat
     8101 agaaataaag aatagataaa caaaattaaa atgaaagagt tttttgatta atttcatagg
     8161 tattgtaagt aagcgataga tttttcttat ttgtcaataa aaaaaagagt tgaaaatgaa
     8221 ttcaactcct ttaattaaat tggattatct aactaattac caaacttctt ctacctgatc
     8281 agccacttta tatcccgggg tctgtttgag cagcgggtct gcattcagcg tagctttgtc
     8341 aattggccac agcactttgt acttttcggt agttttcaat accggtgtgg tagtgtatgc
     8401 cgatgcagca tcaaatgcca acggctgacc gtttattccg tctttcagtc gaagtatatc
     8461 aaaccaccga cttccttcat aaacaaattc tttgtctctt tccttcagaa ttgcatactc
     8521 attggtcttg aaatcggcat tgacatatgc atctgtagcg gctacataat cagcgccata
     8581 agcacgttgg cgtatctggt tgatgtactg agcaggatct ccacccttca tattttccac
     8641 ttcagcgagc ataagcaaca catcggcgta gcgatagatc ggttcgttgc aatcatatac
     8701 gcgagcacca gttgaattta tagaaccaat gaactttttc ataacagtac ccattaaagc
     8761 actacttgga gtgtagtaat ccataaatgt tgcacttctt ctggtatctt tatcactgta
     8821 cgatttataa aaatcaagtg tatattcatt acgttgaatg ccagtcgata atatttttaa
     8881 tgggtcaaca actttagtac aggtttttgt ataaaatgta ttttgtgcag cagcatataa
     8941 gaaatttcct acattacttg ttgcttcacc atcggcataa tatattgcca taatgatttc
     9001 tttatttccc tcattgtcgg atgtgaagat ttttgaaaaa tcggataata atccgaagcg
     9061 agtgttgttt ttaatgtcat taaggtaaga ttcagccttg gctaagtctc cggcatcggg
     9121 tgtttgattt cccaatgtaa cttttgcaga ccaaaggtat gtttgagcag ctaacatttg
     9181 agtggctgct tttgaccagg taacattgtt tgtccattct tttcctgctg aaacaaagta
     9241 atccagtgat ttttgaatat cggatttgat aaaatccatt gtttccttgg gattcgcacg
     9301 tgcagtatac aaatcaactg cattaacttg cccgtcaagc accttaacac gagttactaa
     9361 tggaacacct ccaaatgttc tgtaaagtgt aaaataataa aacgcacgca ttccgtaagt
     9421 ttgagccaat aaataatcca catccttttg gttgttttta tgaatatcgc tgttttctat
     9481 gttttgaatc atcagattaa aatcaaatat ttttccataa aaatcaaccc aaccagatac
     9541 accgggtgtg ttaccattaa catcattctt tttaatatca ccatagtcag gtgcaattcc
     9601 ctgagaacta tttcctgtta ccaacaatcc tcccctggct tcgcctaata tataatttct
     9661 attgaaactt gtactacgta aatccatgtg catgccattc acgtatgctt gagcttgtgg
     9721 aagggttttc caaaaatttg cgttaccata attgtcgatt ggacctaaat cgagattaca
     9781 tgcagtaaaa gtaagtatca aactgaccat tagtgcagct aagtatgtta ttttttttgt
     9841 tttcatttaa gttttttgtt tagagtttat taaaaactaa tgtttaaacc gaatgttact
     9901 gttcttggca ggctatatcc ggcgtcaaca attcctccac ctttttcagg attggcaaaa
     9961 gttttacctg cagttaaata acccaaattc tgacccgtca cagagatatc taacttttga
    10021 ataaatcctt tgttgattat ctcgcgtggc accgaatagg ttaatgaaat ctgacggaac
    10081 gacaagtagt cgcctctgtt cgtaaatagc gtagagcttc ttaaatagtt tccttttcct
    10141 aattggtcgg cataagtata tctgggatat ttagcatcag ggttctctga tgtccatgtg
    10201 tttgtaactt ccataggcat tgcataagtt ccttgcatat tacccattaa ccatggcagg
    10261 cgataatcca tcatccagaa accaagcgca tagtcaaatg ccgtataaag cgataagttt
    10321 ttccaactta aagtagaatt gataccgcca atccagtgcg gagtagtatt ccccacttta
    10381 actttgtcat attcgtcaat tatattatcg ccattaatat ctttccattt tacatccccg
    10441 ggttgaatag gatagtttga agttcctcgg tctgtttcac tcattgcagc ccattgtgct
    10501 ttaccccaaa gagttttaga agggccacca aaggctccca catagttata agaaaggact
    10561 ttcatattgt caggaatttc actttcgttt ttatatattc catcagcttg ccaaacataa
    10621 agcacgccgg gttcctgacc ttcctgatat cctcctaccc atagtaaatc ggttgcatct
    10681 ttacctgaat acacctggaa agcttgctga cgattaagag ggaggccgtt gttcggtaat
    10741 tttacaactg tattcttgtt gtatgcgata ttggcattga tactccagtt ccagttcttg
    10801 ctttgaagtg gtttagctgc caattctatt tcaagacccc tattacggaa ctgaccattg
    10861 ttggtacgga tgctgcttat accagaggaa gatggtaaac tcaagcttgc aaatttatca
    10921 gatgttaagc gatcatataa agtaaagttt gttgaataac ggttttcaaa ataacttaaa
    10981 tccaatccca cttcaaaagt acgcgtcttt tcccaaataa gtcctggatt aggcacactt
    11041 gataaaatga atgtggattg accattgtac tttgtagaac ctgaatagcc accttgcaat
    11101 tcgtaggctc ctattcctga aacgtttcca tttaatccgt aacttgtttt taattttcca
    11161 aaagaaatta catcttttaa tgggttcata aacgattctt ttgaaaacat ccaacctgca
    11221 gaaaagcctg ggaaagtacc ccaacgattg tcaatcagtc tggaatatcc atcacgacga
    11281 actacagcag ataggatgta tttacccata tagtcgtaat tgacacgtcc aaagaaagat
    11341 agaatgcgct gttgagtatg gtaactgtca atgcttcttt tatctttatc gcttgatgta
    11401 agtgataaat cgcggaagtc atctgtagca gctccggaac cggaagcaga gaatccatta
    11461 taaaaagaat cgtaaaattc tgttccaagc atcacagcca gataatgact atttatttgt
    11521 ttttcgtaat ttaaaaccgc attgtatgtc tgttcaaaag aacgattaaa ctcagcactt
    11581 gttgatctgt ttgtgctaat ctgtgtcgga gtgttatagt aatctttggt aaaactttcg
    11641 taatttcctt cactgtaata ccagttggct gccattctta agtagagaga tttagtgaaa
    11701 tctaccttga aagcctgatt catagtaaat ttatccgttt ggttgaagat aaaccattga
    11761 ttgggctgga aattctgatt tccatctcct gcattgttgc ctaatcgtgg tgtcccatcc
    11821 tcgttagaaa atcgtactgt aggaggaact gataaaattc tactaaaata atttgcttcg
    11881 tctccctggg agccgggcat actttgccaa ttggcacgtc cgtaattgat gcttgaaata
    11941 gaagttaacc acggtttaat tttataatcg ccgttgaata taaaagagta tcttttataa
    12001 taggagttaa taggtaaacc ctgagaatag ttataaccta taccggagta atagtgaccc
    12061 ttgtcatttc cacctgacat gtttacgtta taatcttgtg tataagaagg atccttgaaa
    12121 ttatagtctg ccggattggt gtttttgaat attaaatatt cttgcggaac aaatgttgtt
    12181 ccaaattgac ttttgtacca gggtttttga taatttgcaa gcataaaatc ataagcgggg
    12241 tctctcattt tttgccatcc ctgatctaac aagattttat tttgatcatt gagatacatt
    12301 gtactccata tggaatttcc atcatttaga atagttttac catcagatgc aaaatagctg
    12361 tttcctgtgc ccataggggt gttttttgtc aaattggcaa ttggtgaaaa tccaacagga
    12421 gttccatctg cttttgtcca tacattatgt gaatttagat aagcattgcg ttgccaggtt
    12481 agataagttt ctgcatctgt aaatttgtac ggattaatta aattattaac acccaccttc
    12541 gctttgaagt taatttcaga ctgaccactt ttaccttttt tggttgtaat cagaatcact
    12601 ccattattgg cacgagcacc ataaatggct gtagctcctg catctttcat tacctgcatg
    12661 tcttcaatgt cttcggggtt gatgtcacta agactaccgc gaatttgacc atctacaatt
    12721 accagtggag aaccactacc atccaggttg gtaccacctc gaagtgtaat ggttggagca
    12781 gctccggggt taccagaagt ttgagttaca cgtaatccgg atacggcacc ggataatgcc
    12841 tgacctggat tggagaatac cccaacggtc aatttcttgt tatctacttt ggcaatggag
    12901 ttggtgcttc gggagcgaac ctgtgtacct gtgtaccccg taattacaac ttcgtcgagc
    12961 aaagctgcgt cgtctttgag cgataccagg ttggggctgc cgaaacttgc tttgattttt
    13021 gctgaagtat aacctacata cgatacctcc agggtggctc catcggcaac attcagggtg
    13081 aagttaccgt caaaattagt cagtgtaccg ttcgttgtac cagcttcacg tacagtggcg
    13141 ccaatcagcg gctctttgtt cgtatctgtt actgttccgg taactgcttt cttttgagct
    13201 gtagctgcaa gggtaagtag cagcatacaa attaaaatta gctttttttt catctgttag
    13261 attttaagaa ggtgaataat tagattgatt aatgatattt attttgagcg taataaattc
    13321 acttaacata agacaaatgt ttatttgccc atttaatatt atttaattct actcaaattt
    13381 aaatcgcaaa gtaaataaaa ttattttata aaacaatgcg ggtgatgcat ttttatttgt
    13441 cgaatgttcc aagtaaaatt aaaataccat aaaatatgat tttaacttat taaaaatgcg
    13501 ttatttattc aattcttggt atttggaagg gatttagtat atttgcaact aaaatatttg
    13561 gttaacctat ggataatgca gaaaaaaaga gactttatta cactttgttt ttcccgattc
    13621 tattttgctt gtttattatt cttgtttatc tttttgaaca ggcaatgggg tgggactttc
    13681 ataaagcagg catattcccg catgagctga aaacattgcc aaacgtattc acgatgccat
    13741 ttattcattc cgatttatcg catttgttga ataatattat ctcctttttt gttttaagca
    13801 gttgtttgta ttatttttac agccagattg caaataaaat attacttttg tcggtcgtat
    13861 ttagcggatt gatactgtgg gtgattggtc gtgaaagttg gcatatcggt gccagcggtg
    13921 ttattttcgc tatctcattc tttctttttt tcagtggcct tctgcgtaaa catattccgc
    13981 tgatagctat ttcatttgta gtcgtatttc tttacgggaa taatgtttgg catctttttc
    14041 cctggagtaa gatcgacccg gtatcgtggg aagggcatct ggcgggtgga ataacgggtg
    14101 tgttgctggc ggtcatttac cgcaggcagg gaccccaaaa accggtaaag gaatgggaag
    14161 atgatgatga aaatgaagat gatgaggctg aatatcagga taatgacccc gaactgaata
    14221 aggagaattt atcagaaaac taaaatatat taactattaa actatggaaa atagcaaacc
    14281 gaacgaagtc aatttattgg atatcttagt tgtgagcggc aagtggatta aaaacctttt
    14341 taaaggttta ctccgttttg ccggttatat tttaaaatta ttgtaccgca ataagattct
    14401 gaccgcaatc atatttttac tggctattgt tatcggacag tatatggcca gaccttctgc
    14461 ccgaaaattt aatgtggatg caatgtgtgt tttgtatggg cccgaagcaa ccaccatcag
    14521 tgaagtagga aagcagctct cgctttcttc accccgatat gaggcaacat ccctggcaaa
    14581 taaattatct cttccggata gtatcgcttc caaagtggct ggtgtggaat tttttcaggt
    14641 gattgattat caactggatc atactcccga tgagattgac ttcagcaaca gccattcgct
    14701 ggaagatacc ctgaatttgc cgatgagaga ttatatttat atgcggataa aagtgaaggg
    14761 tacacgtcat gcacaagcta tcggtgaggc tatgttgaag tatttcaata caaatccgac
    14821 gatacaaaat cagtataata tattcaaagc gcagttaaat gaaaaagtta atttgtgcga
    14881 tgctgaaatg ttacgcctcg acagtctggc aaaaattacc tacttcaggg ataaaaaacc
    14941 ggaaatcaaa tttgaaagta ataaattgtt gattggaaat aatgaggttc aattgtttta
    15001 caatgatttg ttaaatcttc aggctaacaa agctcaggca atggcacaat tggaagttgc
    15061 caaacagccg gttgttatac cctcgggatt tgtgataaat cccaattcag tcaacggtag
    15121 ggtaaaatta ggagtttaca gtttgtttat aggtttgatt ataagcacta tactcggtta
    15181 ctacatcgaa aaccgaaaaa aatggctgaa atatcttcag gaaaaataag ctgagaataa
    15241 aatcaaaaaa aaggcatctt ttcgatgcct tttttttgat gattaattgc tgaaaatgat
    15301 tttcccattt atgcaatcca caatgattgg ctgctcattg tttaccttac cgctgataag
    15361 ttctttggat aagtcgttga gtacgtaccg ttgtatagct cttttcaccg gtcgggcacc
    15421 aaattgcggg tcatatcctt cagcagcaat aaattcaagt gctttttcgg ttatttcaag
    15481 tttaacccca ttttcagcca gcatattttt tataccgttc aattgcattt tcacaatttc
    15541 agttatctga gattcgtcaa gcggggcgaa catgattatc tcttcgatac ggttgagaaa
    15601 ttcaggtcgg atggcttttt tgagcagttc aaaaacctca tttttagttt tttctatgat
    15661 ttcattccgg ttctgattgt tgatttgctc aaagttttca cgtatcaacg tagagcctat
    15721 gttagaagtc atgataataa ttgtgttttt gaaattgact gttcgtccct tattgtcggt
    15781 cagccgcccg tcgtccagca cctggagcaa ggtattaaac acatcgggat gtgctttttc
    15841 aatttcatcg aagagtacta ccgaataggg ttttcggcgt attgcttctg tgagttgtcc
    15901 gccctcttcg tagcctacgt agcccggagg tgacccaatc agacgcgtaa ccgagaattt
    15961 ttcctgatat tcgctcatat caatgcgggt catcatgttt tcgtcatcga acaggtattc
    16021 cgccaacgct ttggcaagtt ctgttttgcc tactccggtg gttcccagga aaatgaaaga
    16081 cccaatcggc cgctttgggt cattgagccc ggcacggttg cggcgcacgg catccgagac
    16141 tgcggctatg gcttcatcct gccctactac ccgccggtga agttcctctt ccagatggag
    16201 cagtttttct ttttcgccct gcagcatttt tgttaccggt attccggtcc agcggcttac
    16261 tacgtcggca atatcctgac tgtcaacttc ttccttaatc atggcattgt tgccctgaat
    16321 ttcaaccagc cgggactggt tctctgctat gcggttttct tcggctttta tctttccgta
    16381 acggatttcg gccacccggc catagtcacc ttcacgttcg gcgcgatcgg cttcgtattt
    16441 caggttctca atttcaattt tcgattgttg aatatcttct atcaattctt tttccgacga
    16501 ccatttagca cggtattttt tgctttcctc tttcaggttg gcaatttctt cctggagttg
    16561 ttccagcttt cgcacgtcat tttcacgttt gatggcttcc cgttctattt caagttgctt
    16621 gatgtttcgt tctatcttgt ccagttcttc cggcacgctg ttgacttcca tgcggagttt
    16681 ggctgctgct tcgtccatca ggtcaatcgc cttatcgggc aggaagcggt cactgatata
    16741 ccggttcgaa agttcaacgg cagcaatgat tgcatcatcc ttaatcctca ctttgtggtg
    16801 gttttcatat ttttccttca gccccctgag aatagagata ctcgatgcaa tattcggttc
    16861 atccaccatc actatttgaa aacggcgttc cagtgctttg tctttttcaa aatacttctg
    16921 atattcattc agcgtagttg ctcctactgc ccgaagttcg ccacgggcga gagccggttt
    16981 cagtatattg gctgcatcca tggcgccttc gcttttacct gcacccacaa gggtatgaat
    17041 ctcatcaatg aacaggatta tttccccatc cgatttgatg acttcattta ccaccgattt
    17101 caatctttct tcaaattcgc ccttgtattt ggcacctgca atcagtgcgc ccatatccag
    17161 cgaaaagacc tgttttgttt tcagattttc gggtacatcg ccacgcacaa ttcgttgtgc
    17221 aaggccctcg gcaatggcgg tttttccggt tcctggttcg ccgatgagga ttgggttgtt
    17281 tttggttcgt cggctcaata cctgcaacac ccgtcggatt tcttcatcac gtcctatcac
    17341 gggatccaac tttccggagc gagcacgctc attgaggttg actgcgtatt tttcgagcga
    17401 attgtaggtc tcttcggctg ttgccgagtt agctttcgat ccttttcgaa gttcagcaat
    17461 ggccgattcg agagcctttt cggttacgcc tgcatctttc agcagttgcg ataccgtgtt
    17521 tttaactttc aataaagcca gcagtatgta ttccagtgac acaaactgat ctcccgcaac
    17581 tgtcgaaatc tcggtggcct tacgaagtac ctcgtttgtt tcacggctca gatagggctc
    17641 tccgcctgta acacggggga aactttccag gtttttgtca ataacctgtt ggatgttgga
    17701 tgtgtaaatg cccattttgg caaagagaaa cggaattaca tcttccccct ttgacagcag
    17761 ggctttcatc aggtgggcgg tttcaatggc ttgctgattg ttggcctgtg ccagatcgat
    17821 agcgtgttgc accacttcct gcgatttggt tgtgaaattt gaaaagttca tattctgttg
    17881 ttttttagtt taattttttg gatttaatcg ataataaatt actttcaaaa aacttaccaa
    17941 tctgaaaatc agacaaattg gcataaataa ataaaatttt tcacgaaaat atgccaaaat
    18001 gtcagagaag tcgaagtgga tgtcatttta attattttta gtttactttg agagatgaag
    18061 tgctggtaaa ttatccgaag aatgaaaaaa atgattattt ttgcaggtta aatcttgtaa
    18121 attaggaaga taatgcagaa aattgatttt aacgtaccgg aacctacgct caggcggctt
    18181 ccctggtacc tggcatatac caaattggtg caacaagcgg gcgaatcgta tttgtcatcc
    18241 acccagatag cttccaatat tgctgttgat gcttccaaag tagccaaaga tttgtcatac
    18301 gttcagattt caggacgaac ccgcgtgggt tacgatgtca atgagttggt caatgtactt
    18361 gagacgtttc tgggatttac caattcacac aaagcatttc tgtttggcgt cggtagtttg
    18421 ggaggcgcat tgctccacga cgatgggctc aatcagttcg gactggagat tgtagccggt
    18481 ttcgatgtaa aatacgaact ggcaggtacc agcatacatc gggtgcccat tcatcacata
    18541 gatcatttta tagaattaca aaaacaaacg aaaattaata taggagtgct tacggtgccg
    18601 gtggataaag cgcaggagac cgccgaaatg atgatagacg gaggcataca ggcgatatgg
    18661 aactttactc catttcgcat tcgtgtacca gaaaatatta ttgtgcagaa tacgtccatc
    18721 tatgctcatc tggcagtcat gttcaacagg cttaacgaac tgaattcaaa aaggtaagag
    18781 gtatgaaaat ctttgctatc ggtcaaaact acgccgaaca taataaggaa cttaacagtc
    18841 ccgaccctac agaaccggtc atttttatga aacctgacac ggctgcgctc aaaaacaaca
    18901 agccatttta catccctgat ttcagcagcg aactgcatta cgaaacggag attatagtga
    18961 aaatcagccg gcttggaaaa aatattgctc cgaaatttgc ccaccgctat tacgaggaaa
    19021 taggtctcgg agtggatttt acagctcgtg acgtacagag aaggttgaaa gctgaaggca
    19081 aaccctggga aataagcaaa gcttttgata attcggctgt tatcggtaat tttataccca
    19141 aagaagaatt gggtgatgta caaaatattc actttcacct gaatatcaat ggcaatacgg
    19201 tgcaaaaggg acatacctcc gacatgatat ttcccgtgga tgaaataatt gcctggataa
    19261 gccggttttt taccctgaaa atcggtgaca tactatttac cggaacgccc gtgggagttg
    19321 gcaaggtcaa tatcggcgat catctggaag gatatatcgg tgaaaagaaa atgtttgatt
    19381 ttaaagttaa ataaaatcgc aaacctgacc ctgcgcaaag aaaaataata atcattaaac
    19441 caatccgtta ttaactttgt tatcattctt tctaccggcg gtacatacga aataatttcg
    19501 tttatggcaa cttatacgaa tgacttgacg gaataagcag ctaggtcaaa taaagaacaa
    19561 caaatacagt tgaaacagtc gtgactaaac gaaaatttag ccacacaagg aaatgattaa
    19621 actaacttaa ctattcgttc aaatctaaat taacactcca aattccccct taagggggtt
    19681 agggggcaga aagataaatc aaatttcaca tgaaatataa catattaacc ttcttactgt
    19741 taatgatttc cgtggcgaca ctttcgtcac agaatatttc gcagacaaaa catcagataa
    19801 gatggacggg tatagaaacc tggcaaaccg ataaagcgca aaaaaaagtt ctatcgttta
    19861 ccgatgccag ttacccagcc gaaaatgatt tgccctattt tgtgcaaact atttcgtggg
    19921 ataaaaatta cgacgttgat gcacgaatcg aaaatccggt ttatacctca ttgacctctg
    19981 aggaaataaa actgctcgat aatataaatt tatccgaaac acctgaaatt tccacttaca
    20041 taaccgaaga acgcggcaat tcttacctga atattcagat tttaccattg attaagttgg
    20101 aaggaaaagt gaaaaaactc acaagttttg aactgaatgt aaccaaaact tcaaaatcgt
    20161 tcaaagcacc tgctgccatt cactcctacg tatcttcctc ggtattggca acgggcaaat
    20221 ttgtaaaaat aaaaattaaa gatagcggca tttacaaact gacctacgaa gcgctcaatt
    20281 ccatgggcgt taatcctgca aatgtacgga tattcggcta tggcggagct ttgctcgagc
    20341 aaagttttct gcagcccaaa cccgacgacc tccccgaagt ggctgtgtgg atggaaaaag
    20401 gctccgacgg agtgttcaat gccggtgact atattctttt ttacggtcag ggtattgtaa
    20461 aatggaaata cgatcccgct aaagccatgt ttgttcatac actcaaccat tattccaacg
    20521 atgcatttta ctttgtcact tctgatgccg gtacaggtaa gaagattgct gaccagacca
    20581 tcgatgtgcc tgccgatgca atcgttgccg atgtaaacga attcaccgac taccagctct
    20641 acgaactgga aaaacaaaac ctggctcaaa ccggaaaagt attctatgga gaaacatttt
    20701 cagatgtggt ttcatacaat tttcctttca catttaccaa tatggtaaaa acagcaagca
    20761 ccaaagtccg gattgacgta gctgccatct ccagtgagaa ttccactttt acactcaaac
    20821 tcaatgacga tcagccgcag aatgttacgg tgattaaaaa aagtgtggat aattacgaaa
    20881 tcggtaagga cgccaatgca gtactaaatt tcacgccctc agctgataat ttaattttta
    20941 atcttacgta caataaacca actactgtat ccaggggttt tctcaattac ctggaaatca
    21001 atgcccggcg cagcctcacg atgattggtg cagttatgtt tttccggaat acagataatc
    21061 tgaatacagc tacctacaat cggtatcaac tttcaggagc tggacccaat gtacaaatct
    21121 gggatataac tgaccggttg aatataaaaa aggtgattac caccagatca aataatgtgc
    21181 tcgaattcaa agacacaaat tcgagcctga aagaatatgt ggctattgat ccgacggcag
    21241 cttctgcgtt tcagcagcca accacagtgg gagcagttcc aaatcagaat ttgcacgcgc
    21301 ttgcacctac cgaaatggtt atactcaccc atccgtcctt tgtagtccag gccgaaaaac
    21361 tggcacaggc tcaccgcgtt aaggatgata tgactgttgc agtggtaact accgagcagg
    21421 tttacaacga attttcgtcg ggcacacccg atgccacatc gtaccggtgg gtgatgaaaa
    21481 tgttgtacga cagggcaata gctgcgggta atacatcggc tgctcccaaa tacctgttac
    21541 tgttcggccg gggttcgttc gacaaccgtg gaataattct caattcggga aataatctta
    21601 ttctgactta ccaggccgat atgtcgctcg atgtggtcaa atcgtatgta accgatgact
    21661 atttcggatt tctcgatgac aatgaaggac tgcaaatagc ttctcatctc ctcgacgtgg
    21721 ggattggacg ctttccggtg gtaactcccc agcaggccga tgatgtggtg aataagacca
    21781 tttcatacat ggaaaataag gaaaatggaa actggaaaaa tcagttgtgc ttcctggctg
    21841 acgatggcga tggtgcctta cacatgcgac aggccgatag tattgcatca ctggtttcga
    21901 gatcaaatcc ggggtttcag tataacaaaa tctatctcga cgcctacctg caggaagtgg
    21961 ctgcgagtgg cgaaagttac ccgctcgccc atacaagatt tcacaatatg ctgcgttcag
    22021 gtatttttat gctcaattat accggtcatg ccagtcaact ggggtggacc aacgaacaga
    22081 tattgacaac agcggatgta aaacaaatgt ataataccaa actgccgttc tggataggag
    22141 caacgtgcga ttttatcctt ttcgatgtga aagatatatc ggcgggtgaa catgtggtac
    22201 tcaatccggc cggtggtggt atcggacttt tttcggccgc acgaaccgtg tatgcttcac
    22261 agaacgaaaa actaaaccgc tattttacag tgaatttgtt taccaaaccg aatgggaaat
    22321 attaccgatt gggcgatgtg atggccaggg caaaaaatca ggtgggtact gaaattaata
    22381 aactttccta tacattgctc ggtgatccag cactgagact gaattaccct accgattaca
    22441 atgtggtaac cgaaaaaata aacaatcgta cagtacaggc tgcagataca ctacgggcat
    22501 tgtcggtcaa ttcaatcgaa ggatatattg ccgatggatc gggaaaccgg ataagcgaat
    22561 tcaacggaaa tctggaagtt accatctatg ataaaatgca gaaaatcact accctgaata
    22621 atcacaacga cggaacccta acgtataacg accgtccaaa catactttat tccggcaaag
    22681 caaagataca aaacgggaat ttttcattta cattcatggt gccccgcgat atcaagtaca
    22741 actacggctc cggccgaata agctattatg cgagtgattc actcaagcat aaggaagcac
    22801 agggatacta cgaaaatttc atcgtaggag gtggcaccaa tatagacctc aatgccgatc
    22861 aggctggacc ggaactgaaa gtctatctta attctgaaaa ttttgtttcg gggggaaaag
    22921 tgaatgaatc acccctgttt tttgccagaa tatcagatgt taacggagta aatactgtgg
    22981 gaagtggcat cggacacgac ctgagactcg tggtggacga taatcagttt acatcctata
    23041 cgttgaatga ttatttcgaa gctgaaacca acagttacaa atcgggttcg gtgagatata
    23101 aactgccgct gctgcctgcc ggaaaacaca cactcacttt ccatgcatgg gacttgctga
    23161 ataactcatc acaagccacc ctcgatttcg aggtggtagt ggggcttaca ccaaccattt
    23221 tcaaggtata taatttccca aatccggtgc ggaacgagac ccggtttgtg gtggaacacg
    23281 accgaccgga aacaattctg gaaacaaccg ttgatctgta cgacatgaca ggtcgtaaaa
    23341 tctggtcgat gaagaaaaac agtgttgaaa atctgaaatg gaatcgcgcc gatgcatcgc
    23401 tgcaacaagt gaagccggga atttatatct acaagatttc ggtaaaaacg gccaatagtg
    23461 aaatgacttc aaaagcaaat aaaataatcg taaccgggca ataaatccca aattttacgt
    23521 taatacttaa ctgttttttt tataaatatc taatttctcc aaaaaacaag aacttaacta
    23581 aaattgtacc agaaataaaa ttgaattatc tttgcaacca aaatttttaa aacattacta
    23641 aacaatatat taagaaaact aatgaaaaaa acctatttaa tcttgttctc gttgttatca
    23701 ctttctctta catcattttc acaaacaaca gtcaatgatc aacttaatcc cttaattacc
    23761 ggcgttcctt cattaacaat cactcccgat gcccgtggcg gcggtatggg tgatgttggg
    23821 gtggctactt cgccagatat caattcgcaa tactggaatc ctgcaaaata tgcttttatg
    23881 accagtcctg ccggactttc attctccttt acaccctggc ttcgcagttt aggtatcaat
    23941 gatatcaacc tgagctatct gaccggttac tggaagtttg acgaccttca gtcggtaagc
    24001 ggttcgctgc gttatttctc gctgggtagc gtgcggctca ccgatgaaca gggcaacgac
    24061 ctgaatatgg atgctcaacc caatgaacta gccctggatg tggcttattc acgtctttta
    24121 tcagacaact ggtcagcagc cgttggattt cgctatatcc gttccgatct caacaacggt
    24181 gccaatggtg ccggcggaat agaaatgttt ccgggaaatg catttgctgc agatatagca
    24241 acctactata aaactcctat tgcgatggct accggtgatg ccaatcttgc tttcggtttg
    24301 aatatatcga acattggttc caaaatatcc tatgatcaga acgaaaccag catttttatt
    24361 cccactaact taagattggg tggatctttc gaatatccgt ttgatgatta caacaagatt
    24421 tcgttcaatg ccgatatcaa taaattattg gttcctacac ctgtaaagcc cattggcgaa
    24481 gaaacatatg agtcacagag ttaccttgac cgaaaagcaa tttatgatca gactggtcct
    24541 attgccggta ttttcaagtc gttcaacgat gctccgggcg gaatgtcgga ggaaatgaaa
    24601 gaaatcatgt ggtcggcagg cgcagagtat gcctacaaca atcagttttt tgtacgcggt
    24661 ggttatttca acgaacatgc catgaagggc aatcgtaagt atttcaccgc cggcgccgga
    24721 tttaagctca acgtatttca gctcgatgtg gcttatctga tttctgttgc acaatccaat
    24781 cccctggatc aaaccctgcg gtttaccctt ggatttgacc tcaacgggct gacagaactt
    24841 atgagataat cgtaatccat taaaaaattt ccaaaaacgg ttgtgtcaat caattgatat
    24901 acaaccgttt ttagtttaac ggaattgaaa agtaaatgaa cctatgaaaa tcagagttgg
    24961 atttggatac gatgtccatg catttgctcc cgaccgcgag ttatggcttg gcggggtgaa
    25021 gattgaacac accgt
//
LOCUS       DAOPZK010000013        24545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2038_length_24545_cov_25.632830, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000013 DAOPZK010000000
VERSION     DAOPZK010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24545
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2038_length_24545_cov_25.632830"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PKH68_01560"
     CDS             complement(<1..1244)
                     /locus_tag="PKH68_01560"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HNX11650.1"
                     /translation="MDIQNYPAEPFRIKSVEYVKMISREQREAVIKKAGFNTFLIDSE
                     DVYIDLLTDSGTNAMSDRQWGGMMMGDEAYAGSRNFYHLDETVKEYFGFKYMVPTHQG
                     RAAENILSQIAIKPGQYVPGNMYFTTTRYHQERNGGIFVDIIRDEAHDASINIPFKGN
                     IDLNKLEKLIMDKGADKIAYVCLAVTVNLAGGQPVSMQNMREVRDLTRKHGIKVFYDA
                     TRCVENAYFIKEQEPEFRDKSIKEIVHEMFSYADGCTMSGKKDCLVNIGGFLCMNDEK
                     LFGDAKELVVVYEGMPSYGGLAGRDMEAMAIGLKEAMQYEYIEHRIKQVRYLGDRLLE
                     AGIPIIEPVGGHAVFLDARRFCPHLTQDQFPAQSLAANLYLESGVRSMERGIVSAGRN
                     KTTGENHAPKLETVRLTIPRRV"
     gene            1584..>2174
                     /locus_tag="PKH68_01565"
     CDS             1584..>2174
                     /locus_tag="PKH68_01565"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HNX11651.1"
                     /translation="MKRFGVFLAGVLMLALSLTAQTQTKPDTAKADVKDSIEQLYMNE
                     NLDAREAILFKKLDAKQLMDLESMRLSQEGKNEMPFSGLGLVLICLAPFIMVILIVYF
                     SSKESAKKDQRRYDISMKALELGQPLPQSFFDEPKKRSSQLQNGLVWLGVGLAIVLVG
                     VFTHNHSLYFGLIPAFAGLGILIAYFVEKPKVDTTDK"
     assembly_gap    2175..2274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2275..2477
                     /locus_tag="PKH68_01570"
     CDS             <2275..2477
                     /locus_tag="PKH68_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNX11652.1"
                     /translation="EKNDIFAALKYLRKDEQTAILLCYMEDKTHKEIAKIMNIPLGTV
                     KTNILKGKEKMGKYLTQAGYGN"
     gene            2467..2784
                     /locus_tag="PKH68_01575"
     CDS             2467..2784
                     /locus_tag="PKH68_01575"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HNX11653.1"
                     /translation="METKDDKILKTFFGDKKQEIADNGFSEKVMLRLPQRERKTGWIA
                     PVFALIGFLITFALVDMREVAREIFIALSGIPFYYLLGGFMLIPIVTMLVLYAYKREY
                     NIA"
     gene            complement(2885..3163)
                     /locus_tag="PKH68_01580"
     CDS             complement(2885..3163)
                     /locus_tag="PKH68_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11654.1"
                     /translation="MANRRKLKKTMQFIASELMTDVFFKTLLSPTDITAKADKLAVEI
                     SKMSTEFRARANHYDSKNNPKLVKEYYRKLYSDWNTAVEKVIKDVDEL"
     gene            3307..4791
                     /locus_tag="PKH68_01585"
     CDS             3307..4791
                     /locus_tag="PKH68_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX11655.1"
                     /translation="MNFKNTHHILLIALLGFAAAFTMPSCVDERISADTGLKLEFSTD
                     TLQFDTVFTTIGSSTSQIKVYNPNNKNLKISAIGLGMGASSPYRINVDGATNKNNQFT
                     DIELRAGDSLFIFVEVTIDPLKTNAPVFVKDSIVFLTNSNRQDVKLLAYGQDMEILRN
                     KTILNDSTLTGEKPYLVYGDLVVDTGKTLTLNPGCRLYFHNQSSLVVYGNLIANGTRE
                     QPVLMRGDRTDRIFTDIPYNYVSNQWGGVLLLGEQGKHKLNFVTMNSGYAGVYLANQN
                     RNERPTLEIINCRIHNFLKYGLVVQNADVLVANTEISNTGAYSVYLNGGKHTFIHTTI
                     ANFFNSTNVRIQPSSRENYAALMIMELNRIIPMETVFENCIISGSTTDEMDIMTRFSD
                     QYHGRFSNSYIRKAKPETQPAIFNQISWYDKNKRDTVFVNTYLDSENLVYYNFRPDSV
                     SPARNIGNLETAKKYPFDLNGNNRLEDNKPDAGAYEWQPKSRNK"
     gene            4807..5829
                     /locus_tag="PKH68_01590"
     CDS             4807..5829
                     /locus_tag="PKH68_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNX11656.1"
                     /translation="MKHIYPIVFVLFVCVTAYAQDHEKQNFRIVSYNVENYFDVVDDS
                     LTSDEEYLPEGLRAWNITKYQTKQINISKVIAAIGGWNAPALVALCEIESEKCMRDLT
                     KYSPLKNLGYKYLHHESPDARGVDVALMYQPDMFKPFHDEAIRITFPNTTSTTRDILF
                     ASGILPNNDTLHVFVCHFPSRLGGELESEDRRVFVASAVRQKVDSLMNAVIAPKIVIM
                     GDFNDYPTNSSITDVLRARPLTEEITGKGLYNLAFGLQEKGKGSHKFGGEWGMLDQII
                     VSGSLLNPGNKIFTTQEDMHVFDAGFLLEDDYTNLGKMPKRTYIGMKYQGGFADHLPV
                     FVDLWY"
     gene            5873..6592
                     /locus_tag="PKH68_01595"
     CDS             5873..6592
                     /locus_tag="PKH68_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX11657.1"
                     /translation="MKIQLFIPCYIDQLYPQTGFNALKLLEKAGCEVSYNPNQTCCGQ
                     PAFNSGYWDEAATLAYKFLNDFDPALPVVSPSGSCTSFVIHHYHKLLKDKPGLLQQLE
                     NQKQHIYELSDFLVNVLHFNDFNASFPHKVTYHDSCSALREYGIKNEPRELLSRVNGL
                     QLIEMEECETCCGFGGTFAVKNPDISAAMAEKKVQYALASGAEYIISTEASCLMNING
                     YISKHKLPLKGIHLADVLASF"
     gene            complement(6614..6685)
                     /locus_tag="PKH68_01600"
     tRNA            complement(6614..6685)
                     /locus_tag="PKH68_01600"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6650..6652),aa:Glu,seq:ctc)
     gene            6868..8304
                     /gene="amrB"
                     /locus_tag="PKH68_01605"
     CDS             6868..8304
                     /gene="amrB"
                     /locus_tag="PKH68_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNX11658.1"
                     /translation="MNTDFSNFDRHPAVAGQFYPADARELEKQLERLFQEAVPKNHEQ
                     VRAIISPHAGYVFSGRIAASAFNQIDTSVACQRVFILGSSHHFRFEGASVFCGGDFIM
                     PYGSHAVDLELANELIEKYPALFSDYRPAHYRDHCIEVQLPFLHHQLKGNYKIVPIIL
                     GTNNPEVCREIALALKPYFNPDNLFIVSTDFSHYPSYDDAIQVDKSTKNAILSENPNV
                     LRATLTDQAHKKTPNLLTGLCGWTSVLTLMYMTEKESNLCYEAVEYCNSGDVRHYGER
                     NQVVGYWAIVVKEKSTVEKDEFALTESEKKLLLQEARRTIENLFGTSSIPKPAIISET
                     LQTKCGAFVTLHERGRLRGCIGMIVADKPLVETVRKMALSAARHDHRFMPVTEEELDD
                     IEIEISVLSPLKLIHDVSEIKLGIHGVFIRQGSHTGVFLPQVATETGWSLDEFLGHCA
                     RDKAGIGWDGWKDADIYTFTATVFQEEK"
     gene            complement(8305..9330)
                     /gene="amrS"
                     /locus_tag="PKH68_01610"
     CDS             complement(8305..9330)
                     /gene="amrS"
                     /locus_tag="PKH68_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNX11659.1"
                     /translation="MNADKYQVLSRFQEKVEGNKVRCRICPHNCVIPDGKTGICRTRI
                     NRNGTLLSLAYGNPCSVSIDPIEKKPLFHFFPGEKIFSLATAGCNFRCLNCQNWQISQ
                     SAPDEISNYDLSPRDVIRQAELSGTRLIAFTYTEPTVFYEYMYGTAQTAHSRGMKTVI
                     VSNGYINREPLLELIPYLDGANIDLKCFDNATYHRLTGGQLQPVLDTLKILRENKVWL
                     EITNLLIPSFNDKTEQIRSMCNWLADNGFIDTPIHFSRFFPNYKLTETPPTPIETLIE
                     AKNTAENAGLKFVYIGNTHQPNGENTSCPHCKRTIIGRTGFSVTENHVVKGKCRFCGT
                     TIPGVWE"
     gene            complement(9339..10268)
                     /locus_tag="PKH68_01615"
     CDS             complement(9339..10268)
                     /locus_tag="PKH68_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNX11660.1"
                     /translation="MKRVSALFFLFTVLLALPAQAQKTDNKLTKLIEKEVNGFHGIAG
                     IYVKNLKTGREAVYNADTLFPTASTIKIPIMVGIFNKIVNKELNYYQPLTYSKRKQLY
                     AGDDGLIASLQDSSKVSLSIVQMLSITTSDNSASLWLQELAGTGTEINNLMNSIGLKN
                     TRVNSRTPGREDIREIYGWGHTTPREMAGLLVKIRNREILTPAACDEMYRILGTIYYD
                     DRALSQIPPTVKTASKQGMVSESRSEVVLVNAPHGDYVFAVYTKNQKDLSWKKTNEGR
                     QLIISISKLLWNYFEPTYGWKPAEGYDEFNGAD"
     gene            complement(10539..11648)
                     /locus_tag="PKH68_01620"
     CDS             complement(10539..11648)
                     /locus_tag="PKH68_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX11661.1"
                     /translation="MEDKLVTLAIRTYQRAQMIKTVLEQSGIETQIHNLNLEHPEMAV
                     GVRVRIKESDLPRALVIVEEKEKAWELEESKDAPPRGRKILIPIDLTDQINDVCLYGF
                     QFAEKLYAEVIFLYAYFTPAFTITSNNEINTYSLADSEMLRRIVNTNSADVENLTNLI
                     NKWIATGKIPDVKFRFELKEGVPEDQILEMCKKEQPVMVVMGSRGKNRKDEEVIGSVT
                     AEVLESCNVPVFAVPVDLPVKSPAEISRIAFLTNFDQKDLIAIDTAISFFNREKLELI
                     FIHASQKKDSWDEVMLAGIKEYFSKHYEGIKTEYAILNRDENLDQIQQFLSEANIDMV
                     ALNTKKRNIFARFFNQGIATKLLFNVDTPLLVMHL"
     gene            11868..12269
                     /locus_tag="PKH68_01625"
     CDS             11868..12269
                     /locus_tag="PKH68_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11662.1"
                     /translation="MKKAIWIISLLVLLVLSGVVYYKYYFVFGEGVKAGELNYMVRKG
                     VVFKTYEGKLIQSGFRSSGKTAGSTIQSYEFEFSVENEGIADTLMLSSGKDMELHYRE
                     YKGVLPWRGFSKYVVDKIIKIEKQDPATVPF"
     gene            12419..13579
                     /locus_tag="PKH68_01630"
     CDS             12419..13579
                     /locus_tag="PKH68_01630"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNX11663.1"
                     /translation="MEVTELKKSFKEAYGHEADAVYFSPGRVNLIGEHTDYNGGFVFP
                     CALSFGTYLLIRKNGEKFVQFRSLNQPEVVKVTFDKLTERLENSWVNYPLGVFAQYVK
                     RGVTFEEGFDIMIWGDVPNGAGLSSSAALEVVTAYALNDQLGTGLSRTELAKIGQKAE
                     HEFALVNCGIMDQFASANGKKDHAIFLNCDTLDFELVPVKLEGVKVVISNTHSPHKLD
                     SGAYNQRVAECKLAVEQLNKVRPIKYLAELTEAEFEKVQDAITDPVAHKRARHVVGEV
                     QRTTDAVKALKAGDITLFGQLMNASHVSLRDDYEVTGPELDTMAEEAWKIDGVIGSRM
                     TGGGFGGCTVSLVKDEAIEEFKEKVGAAYEAKINIKPDFYIAEIGNGAMKVE"
     gene            13678..14676
                     /locus_tag="PKH68_01635"
     CDS             13678..14676
                     /locus_tag="PKH68_01635"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNX11664.1"
                     /translation="MIAIYTIKNDKGMSAEITNYGARVVKLWVPDRKGELADVVLSFD
                     TLEEITTKETYFNSTCGRFANRIKDGKFTLDGKEYTLAVNNGPNHLHGGLVGFNMRVW
                     EVKSSSENKIVLHYFSPDGEEGYPGNLDVTVTYELSDENELKIHYEATTDQPTIVGLT
                     NHSYFNLKGAGEGPVRDHILQLNADFHTALDDATCPTGEVLKVDGTPYDFRQPTLIEE
                     RIDDPVYASNRGFDDNWVIRRNQPHELALAGYIYEPKSGRKMEVLTTQPGVQIYAANW
                     VEKQTGKYGKEYDAQCAICLETQGFPASPAYPHFPSPVLRPGEKYDEWCIYKFSAE"
     gene            complement(14744..15742)
                     /locus_tag="PKH68_01640"
     CDS             complement(14744..15742)
                     /locus_tag="PKH68_01640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNX11665.1"
                     /translation="MILVTGATGMVGGHLIWHLLQTNERVSALKRHPDFEECLKIVFE
                     FYTDKPGEYLRRVDWVEGDVLDADSLKTAFEGADYIYHCAAMVSLGKNDESMLQTNVG
                     GTGNVVRAALACKAKKICFVSSIAALSDDTSHGPIDENTPIEPGKSVSTYAQSKLLAE
                     IEIQKGIEQGLNAVIVNPGVILGISAKMTGSGELFKRVKKGLPFYTYGKTAYVDVRDV
                     CRAMIVLMNSEINAERFVLVGENCAHKDILQWMAEGYHVYRPFIGFGKSLIPVAGLLE
                     IMGRIFGFTPLLDRSSALTSISRKEYSSRKFLSTFDFAFTPIRQCIGEMCEFDRHR"
     gene            15833..16411
                     /locus_tag="PKH68_01645"
     CDS             15833..16411
                     /locus_tag="PKH68_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX11666.1"
                     /translation="MQNICVYCASSSKIDSKYFNAAIQTGKILAQNEIRLIYGGGSVG
                     VMGVLADSVLDGGGKVTGVIPQFMIDAAWHHDGLTELIITDNMHERKEKMAALGDAAV
                     ALPGGVGTLEELLEVITWKQLGIYTKPIVIVNVDGYYDHLIAQLQQAADELFMRPDHL
                     KMWTVVESADQILDAIANAHTWNENLRQFAKI"
     gene            16426..17238
                     /locus_tag="PKH68_01650"
     CDS             16426..17238
                     /locus_tag="PKH68_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNX11667.1"
                     /translation="MPVTDSIIKAVDTLSRVKIDSVAVGDSLLRADSIARIDSAKLAA
                     SSLPTGMPGVTHPSLPMNEPWVFILLIMLFILLAASIVKSGGELFQNIRSTFKHNYSW
                     GTSGVSTLNNLQSQILAITCSIGVISLFVFELFFEMSGRFDIKTYGKVVGVTLAFYLF
                     KYLILEAVGRVFLNKKAVQNYLSLYFNFLTLFATALFPVLFLYTFQPPTWKLSLELVA
                     AVLFGCFYVLLIIALFQNFYTKKLALFYFFLYLCTLEIIPLFVLFRVYELIV"
     gene            17266..18027
                     /locus_tag="PKH68_01655"
     CDS             17266..18027
                     /locus_tag="PKH68_01655"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNX11668.1"
                     /translation="MKIKKILVSQPQPTTEKSPYYDIIEKHNVKIDFRPFIKVEPVTA
                     KEFRAQHVNILEFTAVIFNARHGIDHFFRLCEEMRITVPESMKYFCVSETVALYLQKY
                     IQYRKRKIFFSPTGKFPDLIPVLNKHQEEKFLYITSDVRNEETMSILENSKITFTQGI
                     MYRTVSNNFAPDEPFEYDMLIFFSPAGIKSLLENFPNFEQGDIQIGCFGATTAQTVRD
                     AGLRLDLEAPSPGVPSMTMGLDNFLKEAKKNNGKK"
     gene            18122..18529
                     /gene="rnpA"
                     /locus_tag="PKH68_01660"
     CDS             18122..18529
                     /gene="rnpA"
                     /locus_tag="PKH68_01660"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNX11669.1"
                     /translation="MNTFPKKEHLCGDINITRLHELGNAFMSFPFRVVYRMADDKESV
                     PVRVMVSVPKKRFKHAVDRNRIKRLIREVYRLNKQEISDYAIEYNLKLHISFQYVSNE
                     VEKYELLERKMKNALRKLIQNLSVPNQTDENSN"
     gene            18513..18767
                     /gene="yidD"
                     /locus_tag="PKH68_01665"
     CDS             18513..18767
                     /gene="yidD"
                     /locus_tag="PKH68_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNX11670.1"
                     /translation="MKILTKILEYIILLPIYFYRYAISPLKPPSCRYTPTCSEYAVQA
                     VKKHGPFKGLWLATRRILSCHPWGGSGYDPVPDTFSFRKK"
     gene            19031..19711
                     /locus_tag="PKH68_01670"
     CDS             19031..19711
                     /locus_tag="PKH68_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNX11671.1"
                     /translation="MKKQVSVAFMACGLLFTTCLIVANIVEQKLISIGPIEATAGLLI
                     FPVSYIINDIIAEVWGYRKARLIIWYGFLMNFLTVIIFRLSIVVPGSENFSHQEAFQL
                     VLGNTERISLASFIAFLIGSFLNAMVMSKMKIFQKGRNFSLRAVVSTLVGEGADSLVF
                     FTIAFVGVIPNGDLMTLILTQTAMKTGYEILVLPLTNVIVKWVKRKEQEDVFDEGISY
                     NPLKINEL"
     gene            complement(19714..20424)
                     /locus_tag="PKH68_01675"
     CDS             complement(19714..20424)
                     /locus_tag="PKH68_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HNX11672.1"
                     /translation="MRKHSFIIFFLCIIAVIRAQTTPDSLTLSPDDTVTVFSTETIKK
                     STESSGSNAIPQFKYRNNDSPLGVKLAWGMLLNSAQNAAMMTALILGPSEISKWEHPD
                     RLKWYMIKQKYQNAYTKPPVIDPDLWITNYIGHPYQGSLYFNTLRSQGSSFLESSVYN
                     LAQSVLWEYVWEAGLEQPSIQDLIVTPIVGTIMGELSHRATAGMRRNGFNWYEKVLVC
                     LINPYYAVFNGFSNRPSK"
     gene            complement(20725..22269)
                     /locus_tag="PKH68_01680"
     CDS             complement(20725..22269)
                     /locus_tag="PKH68_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX11673.1"
                     /translation="MKKFLPFTLFALFVFSIPVAGQHHPFHQDIKKQTIPGSLNIARS
                     IRDLQSLKTSAVLTTQKIDEIIKAAQAQAVQPELLDSMRYWDWNSDIGNWVNDEKTIM
                     MTYDMKNRLVSAINQQWDIDAGEWVNEIKYTYTYNSNDVMTSVLWESWDGEKWVNSMR
                     STMTYDANKNMITSKTEMIFMTMWMTFTFTTMTYDANNNLLTQITQSGITGTLVNDSK
                     EIYTYNATNHKLTSLKQTWVTDQWVNSTYTVNTYTSKGNVLTITNQVWDAGAWLNTDK
                     RTYTYDTKENITNILNQIWNSGWKNQSQWISTYDTRQNEISSIRQIWENGAWVNNSKN
                     SYTYSATDELLTDLHQNWGANDWVNDVLETRTYDGNGNNYRTEYQTWLNNAWYRSALD
                     VETYDSNNYPITYSSKLFLENGITVSNGDSTRYYYRPATGVYNLSDDQITVYPNPGKG
                     KFVVSSSENIDGIEIFNSVGEMVLDDSKIGNQLKSFDLSHYSKGIYVIRINTGNKFFT
                     KKLILQ"
     gene            complement(22401..>23174)
                     /locus_tag="PKH68_01685"
     CDS             complement(22401..>23174)
                     /locus_tag="PKH68_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNX11674.1"
                     /translation="CMKAKFLLLVFVVGLTLPAVAYDAFGHRIVATIAYQNLTCHTRH
                     KVDKLLGKHGLTYESTWSDDIRSDSAYAYSYDWHFQNLREGMTPADIQTLFQNPTAEG
                     RHLFFAIDSLSGSLKKNKKDLEALKFLIHFIADAHQPMHLGRAEDRGGNDVMIKWFGK
                     DIRLHQLWDTQMIEGHNFSYSEYTAYLQDKFAPRKKEFKQQSLPQSVEAVYLVRNKIY
                     AYNYTNLRAYTYMYQFNDDLDEMLYRGGIQLANLLNEIF"
     assembly_gap    23175..23274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23275..24182
                     /gene="xerD"
                     /locus_tag="PKH68_01690"
     CDS             <23275..24182
                     /gene="xerD"
                     /locus_tag="PKH68_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNX11675.1"
                     /translation="SLLDEYHFYLKMEKSLSENTIEGYERDLQKLFDYLAEAHIQPQD
                     AENRHLRDFIIEIAEIGINERSQARLISSIRSFYKFLIYKDKITVDPTELLQSPKIGL
                     RLPEVLTLSEIDRIIENINLSKPEGQRNKAIIEVLYGSGLRVSELISLNLSNIYFDEG
                     YMLVLGKGNKQRLVPLSDESKKQIGFWIMDRNHLNIKKGNEDFLFLNRRGSKLTRAMI
                     FTIIKELAVMAGIHKNVSPHTFRHSFATHLLENGANLRAIQQLLGHESITTTELYTHI
                     DVQFLRQTILECHPMNQKNKKHEVE"
     gene            24169..>24545
                     /locus_tag="PKH68_01695"
     CDS             24169..>24545
                     /locus_tag="PKH68_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX11676.1"
                     /translation="MKLNNSFLHRITKRILLSGVAFLFLSTGTLAQETVVVGQVLDKY
                     DKSPISTVDIYFKNSNRAVQSNDDGYFLIRNNGPETTLIFTIVGYKPYELKLKRGDNV
                     GVQIELEEKKNILDEVLVLPGINP"
BASE COUNT         6932 a         5006 c         5216 g         7191 t
ORIGIN      
        1 tacacgcgac gtggaatagt cagacgaact gtttcgagtt tcggagcgtg attttcaccg
       61 gttgtcttat ttcgcccggc ggaaacaata ccccgttcca tgctgcgaac gcctgactca
      121 aggtacagat tggctgccag actttgcgcc ggaaactgat cctgagtgag gtgcggacag
      181 aatcgccgtg catccagaaa tacggcatga cctcccacgg gttcaataat tggtattccg
      241 gcttccagca agcggtcgcc gaggtagcga acctgcttga tgcggtgctc gatgtattcg
      301 tattgcatgg cttctttcag acctatggcc atggcttcca tatcgcgacc ggccagtccg
      361 ccgtaagaag gcattccttc atataccact accaattctt tggcatctcc gaatagtttt
      421 tcatcattca tgcacagaaa accgccgata tttaccagac agtccttttt accgctcatg
      481 gtgcagccat cggcgtagct gaacatttcg tgaacgattt ctttgatgct tttatccctg
      541 aattcaggtt cctgttcctt gataaaatag gcattttcca cacagcgggt ggcgtcgtaa
      601 aataccttga taccgtgttt gcgggtgaga tcgcgcactt cacgcatatt ttgcatcgaa
      661 accggttgac cgccggcaag gtttaccgtt accgccagac acacataagc aattttatct
      721 gctcctttat ccattatcag tttctccagt ttattcagat cgatatttcc cttaaaggga
      781 atgttgatac tggcgtcgtg tgcttcgtcg cggataatat ccacaaaaat tcccccgttg
      841 cgttcctgat ggtagcgggt ggtggtaaaa tacatattcc cgggaacata ttgtccgggt
      901 ttaatggcaa tttgtgacag aatattctcg gctgcccgtc cctgatgggt aggaaccatg
      961 tatttaaatc caaagtattc tttcaccgtt tcgtccaggt ggtaaaaatt gcggcttccg
     1021 gcatacgctt catcgcccat catcatgcct ccccactgcc tgtcgctcat ggcattggtt
     1081 ccgctgtcgg tgagcagatc tatgtaaacg tcttcagagt caatcagaaa ggtattgaaa
     1141 cctgcttttt ttattacagc ctcacgttgc tcgcgcgaga tcattttaac atattccaca
     1201 cttttaatgc ggaaaggttc tgccggataa ttttgaatgt ccataacacg gtatttttta
     1261 taaaggtttt tttattacga ttgccttaca aatgtatagt gaaatgacga aatatcaaag
     1321 aataattgta aatatatttt atttatatgc aaaataacgt ctttggtctg acagtatgct
     1381 atttttatgg ttgtgtgata gttttattta cccggtagta attttgattt tttatcgctg
     1441 attaagtttt tgacttccac attttttatc cgtaaatgtt aaatattgga atttttctct
     1501 tgttttaaat ccaaactaca tttcgtttcg tctaattcat aaacaaagat taaatttaca
     1561 aaccaattaa attgaaaatt aaaatgaaac gatttggagt ctttttagca ggagttttga
     1621 tgctggcctt aagcctgaca gctcaaaccc aaacgaagcc ggatacggct aaagcagatg
     1681 tcaaggatag tattgaacaa ctgtacatga acgaaaacct ggatgcacgg gaagcgatat
     1741 tgttcaaaaa gctggatgcc aagcagctaa tggatctcga atcaatgcgt ttatcgcagg
     1801 aaggtaagaa tgaaatgcca ttctcgggac ttggattagt actgatatgt ctggcacctt
     1861 ttattatggt aatcctcatt gtgtactttt catcgaagga gagtgccaaa aaagatcagc
     1921 gccggtacga tatttccatg aaagcacttg agcttggtca gccgctgcct caaagttttt
     1981 ttgatgaacc aaaaaagcgc tcttcgcagc tgcaaaacgg actggtatgg cttggagtgg
     2041 gtctggctat tgtgttagtg ggggttttca cacataatca ttctctgtat tttggactaa
     2101 ttccggcttt tgcaggactg ggcattttga tagcttattt tgtggaaaag ccaaaagttg
     2161 atacaacaga caagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgaaa
     2281 aaaatgatat ttttgcagcg ttgaaatacc tgcgcaaaga tgaacagacg gcaattctgc
     2341 tttgttatat ggaagataag acacacaagg aaatagctaa aatcatgaat atcccgctgg
     2401 gaacggtgaa gacgaatata ttgaaaggaa aggaaaaaat gggaaaatac ttaacacaag
     2461 ccggctatgg aaactaagga tgataaaata ctgaaaacat tttttggcga taagaaacag
     2521 gaaattgcgg ataacggatt ttcggaaaaa gtaatgctcc ggttgcccca aagggaacgc
     2581 aaaacaggct ggattgcacc ggtttttgca ttgatcggct tcctgattac atttgccctg
     2641 gtggatatgc gggaagttgc gcgtgaaata tttattgctc tctcaggaat tccattttat
     2701 tatctcttag gtggttttat gctcattcca atagtaacca tgctggtgtt atatgcttac
     2761 aaacgggaat acaatattgc ctgaaaacta actaataaat aacagcagtg ggaaatccga
     2821 acgggtttcc cactgctgtt tattggtgtg aataatttat tgtcagccag atagaaaaaa
     2881 tcacttataa ctcgtccaca tcctttatca ctttttctac ggctgtattc caatcggagt
     2941 acaactttcg gtaatactcc tttacaagtt tcggattgtt tttgctgtcg taatgattgg
     3001 cgcgggctct gaattctgtg gacattttcg aaatttcaac agccagttta tcagcctttg
     3061 cggtgatatc ggttggggaa agtaacgttt taaaaaacac atcggtcatc aactcagaag
     3121 cgatgaactg cattgttttt tttagttttc tgcggtttgc catgattttt gtttgtttga
     3181 ggagtgaata attgttacaa atatagctga taattataat tgtagcatct tttttgagta
     3241 aatttgccgg aaaataattt gcagttgttt gcgttttgtc agtgtaatcc ggtcatatca
     3301 ttgtacatga actttaaaaa tacacatcac attctcttaa ttgcacttct cggatttgca
     3361 gccgccttca cgatgccgtc gtgcgtggat gagcgcattt cggctgatac aggtctgaaa
     3421 ctcgaatttt cgactgatac acttcagttt gatacagttt ttaccaccat cggaagctcc
     3481 acttcgcaga ttaaagtcta taatccgaat aataaaaatc tgaaaatttc ggccatcggt
     3541 ctgggaatgg gtgcaagttc gccctacaga ataaatgtgg atggcgcaac caacaagaac
     3601 aaccaattca cagacataga gctccgggct ggtgacagtc tctttatatt tgtggaagtg
     3661 actatcgatc cgcttaaaac caatgctccg gtttttgtaa aagattccat cgtttttctt
     3721 accaactcca accgacagga tgtaaaactg ctggcgtacg gacaggatat ggaaattctc
     3781 cgaaataaaa ctatcctgaa tgacagtacc ctgacgggcg aaaaaccata tcttgtttac
     3841 ggtgatttgg tggtggacac aggcaagacg ctgacactaa accccggatg ccggctgtat
     3901 tttcataatc agagtagtct tgtggtatat ggaaatctca ttgccaatgg tacacgcgaa
     3961 caaccggtgc tgatgcgtgg cgaccgtacc gaccggattt ttacggatat tccttataat
     4021 tatgtttcca atcagtgggg tggtgtattg ctgcttggtg aacagggaaa gcacaaactc
     4081 aactttgtga ccatgaacag tggttatgca ggagtttatt tagccaacca aaacaggaat
     4141 gagcgtccga cacttgaaat cataaactgc cggattcata attttctgaa atacggattg
     4201 gtagtccaga atgccgatgt gctggtagca aatactgaaa tttcgaatac cggtgcatac
     4261 tcggtgtacc tcaatggcgg aaaacatacg ttcatccata ctaccatagc caattttttc
     4321 aacagtacca atgtgcgtat tcagccctcc tcgcgcgaaa actatgcagc cctgatgatc
     4381 atggagttaa accgcattat tcccatggaa acggtttttg aaaactgtat aatttccggc
     4441 tccactaccg atgaaatgga tattatgacc cggttttcgg accagtatca cggccggttc
     4501 agcaacagtt acatccgcaa agcaaaaccc gaaactcaac ctgccatatt caaccagatc
     4561 agttggtacg ataaaaataa aagagatact gtatttgtaa atacttatct ggatagcgag
     4621 aacttggttt attacaattt cagaccagat tcggtctcac cggcacgcaa tatcggcaat
     4681 ttggaaactg ccaaaaaata tccctttgac ctcaacggaa acaatcgcct ggaggacaac
     4741 aaacccgatg caggagcgta tgaatggcaa cccaaaagcc gaaataagta accaaaaacg
     4801 ccaacaatga aacatattta tcccatcgtt ttcgttttgt tcgtttgtgt tacagcctat
     4861 gcgcaggatc acgaaaagca aaatttcaga attgtaagct acaatgtgga gaattatttc
     4921 gatgtggtag acgattcgct gaccagcgat gaagaatatc tccccgaagg cttgcgggca
     4981 tggaacatca ccaagtacca gaccaaacaa attaatatat cgaaggtaat tgcagctatc
     5041 ggaggatgga atgctccggc tttagtcgca ttgtgcgaaa tagaatcgga aaaatgtatg
     5101 cgtgacctga ctaaatattc tccgcttaaa aatctgggtt ataaatacct gcatcacgaa
     5161 tcgcccgatg cccgcggcgt tgatgtggca ctcatgtatc agcctgatat gtttaaaccc
     5221 tttcacgacg aagctattcg tataaccttc ccaaatacaa catccacaac ccgcgacatt
     5281 ctatttgctt cgggcatatt gcccaacaac gacacactgc atgtgtttgt ttgccatttt
     5341 ccttcgagac ttggtggtga acttgaatca gaagatcgcc gcgtgtttgt ggcttctgcc
     5401 gttcggcaaa aagtggacag tcttatgaat gctgtgatcg ccccgaagat tgtaatcatg
     5461 ggtgatttta acgattaccc caccaacagc agcatcaccg atgtgcttcg ggcacggccg
     5521 cttaccgaag aaattaccgg taagggactt tacaatctcg ctttcggact gcaggaaaaa
     5581 ggtaaaggct cgcacaaatt cggtggcgaa tggggcatgc tcgaccagat tattgtttcg
     5641 ggcagcctgc tcaatccggg caacaaaata ttcaccacgc aggaagatat gcatgtgttc
     5701 gatgccggtt ttctgctcga agacgattat actaatctgg gtaaaatgcc aaaaagaacg
     5761 tacattggaa tgaaatatca gggtggattt gcagatcact tgcccgtttt tgtggatttg
     5821 tggtattgaa attaaagcat acagtgtacg aataattcaa caagaaatat tgatgaaaat
     5881 acagcttttt attccctgtt atattgatca gctttaccct caaaccggtt tcaatgccct
     5941 taaactgttg gaaaaagcag gttgtgaagt cagctacaat cccaatcaga cttgctgcgg
     6001 acaaccggca tttaacagtg gctactggga cgaagctgcc actctcgcat ataaatttct
     6061 gaatgatttt gatccggctt tgcctgtcgt ttcgccttca ggctcatgca cctcttttgt
     6121 cattcatcat tatcataagt tacttaaaga taagcccggg ttactccaac agctcgaaaa
     6181 ccagaaacaa catatctacg aattgtccga ttttctggtg aatgtgctgc atttcaatga
     6241 ctttaacgct tcctttcccc ataaggttac ctaccacgat tcctgttcgg cactgcgcga
     6301 atacggcatc aaaaatgaac caagggaatt actcagtagg gtaaatggtc tgcaacttat
     6361 tgagatggaa gaatgtgaaa cctgctgcgg atttggggga acctttgccg taaaaaatcc
     6421 cgatatttct gctgctatgg ctgagaagaa agttcaatat gcactggcat cgggggcgga
     6481 atacatcatc agcaccgagg cttcgtgtct gatgaatatc aatggttata tcagtaaaca
     6541 caaacttcca ctgaaaggaa ttcacctggc cgatgtgctc gccagtttct gatttctttg
     6601 aaaaaaaatc ttgtagtgcg tagcagaatc gaactgccat tgtcggattg agaaaccgat
     6661 ctcctaaccg ttagaggaac gcaccaaatc tgttgtttcc ggaaaattca atcacaaaaa
     6721 taaaccattt tcggaaaaat tgtaattctt ttctcaaatt atttcgctta aaaaaatcca
     6781 acttgtaatt gatcaaccag ttttacattc gcgttcaaac ttatcagcct ttctatccgt
     6841 tttaatagag aaaaaagact tgaaattatg aatactgatt tttcgaattt cgatagacat
     6901 cctgctgttg cgggacaatt ctatccggct gatgcccgcg aactggagaa gcaattggag
     6961 cgccttttcc aggaagctgt accaaagaat catgaacagg ttcgtgccat tatcagtccg
     7021 catgcagggt atgtgttctc aggcaggata gctgcttccg ctttcaatca gattgacaca
     7081 tctgttgcct gccaacgggt ttttatactg ggttcgtcgc atcatttccg gtttgaggga
     7141 gcttcggtct tttgcggcgg tgattttata atgccctacg gcagccacgc ggtggatttg
     7201 gaattggcta atgaactcat cgaaaaatat cccgctttgt tttccgatta tcgcccggca
     7261 cattatcggg accactgtat tgaggttcaa cttccctttt tacatcatca gttgaaaggc
     7321 aattacaaaa ttgttccgat aattttggga acgaataatc ctgaagtttg tcgggaaata
     7381 gcgttggcat taaagccgta tttcaatccc gataatctgt ttattgtgag taccgatttt
     7441 tcacattatc catcgtacga cgatgccatt caggttgaca agtctacaaa aaatgccata
     7501 ttatcggaaa atccgaatgt gttaagagcc accctgaccg atcaggcgca caaaaaaaca
     7561 cccaatctgc tcaccggttt gtgtggatgg acttcggtac tgacactgat gtacatgacc
     7621 gaaaaagaaa gtaatctgtg ttatgaagcc gtggaatact gtaattccgg cgacgtgcgg
     7681 cactacggtg aacgcaatca ggtggtgggc tactgggcga ttgttgtgaa agaaaaaagt
     7741 accgtggaaa aagatgaatt tgcattgact gaatcagaaa aaaaactatt gttgcaggaa
     7801 gcccggcgta caatcgaaaa tctttttgga acatcctcaa tacccaaacc agcaattatt
     7861 tcagaaactt tgcagacaaa atgcggtgct ttcgttacgc tacatgagcg tggccggttg
     7921 cgcgggtgca tcgggatgat tgttgccgac aaaccacttg tggagacggt gagaaaaatg
     7981 gctttgtcgg ctgccaggca tgatcatcgt ttcatgcccg taactgaaga ggaattggat
     8041 gacattgaaa tagaaatttc ggtactttca ccgctgaaat taatccatga tgtatctgaa
     8101 attaagcttg gaattcatgg cgtttttatc cgccagggaa gtcataccgg agtattttta
     8161 ccacaagtgg ctacagaaac agggtggagc ctggacgaat ttctgggaca ttgtgcccgc
     8221 gacaaagccg gtatcggttg ggatgggtgg aaagatgccg atatttatac gtttacagct
     8281 accgtttttc aggaagaaaa gtgattattc ccatactccg ggaattgttg tgccacaaaa
     8341 ccggcatttc cctttcacga catgattttc ggtaacagaa aatccagtgc gcccaataat
     8401 cgttctttta caatgaggac aactggtgtt ttcaccgttc ggttggtgag tattaccgat
     8461 ataaacgaat ttcaatccgg cattttctgc cgtgtttttg gcttcaatca atgtttctat
     8521 gggtgttggc ggcgtttcgg ttaatttgta gttgggaaaa aaacgactga aatgaatggg
     8581 tgtatcaata aaaccattat ctgccagcca gttgcacatt gagcgaattt gttcggtttt
     8641 gtcattgaac gaaggaatca gcagattggt tatttccagc cagaccttat tttcacggag
     8701 aattttcaat gtatcgagta ccggctgaag ttggcctccg gtaagtcggt ggtaagtggc
     8761 attgtcaaaa catttcaaat cgatatttgc tccatcgaga taaggaatca attccagcag
     8821 cggttcccgg ttaatatacc cgttggaaac gatcacagtt ttcattccac gtgaatgcgc
     8881 tgtttgggca gtaccgtaca tatactcgta aaaaaccgtg ggttcggtat aagtgaaagc
     8941 tatcagtcgg gtgccggata attccgcctg acgaatcaca tcccggggtg ataaatcata
     9001 attgcttatt tcgtccggtg ccgactggga tatctgccag ttttggcagt tgagacagcg
     9061 aaagttacat ccggcagtgg caagagaaaa aattttttca cccggaaaaa agtgaaacag
     9121 cggttttttt tcaatgggat cgatactcac tgagcacgga ttgccgtacg cgagggacaa
     9181 tagcgtaccg ttgcggttaa tccgcgtgcg gcaaataccg gtttttccat cgggtatcac
     9241 acaattatga ggacaaatgc ggcacctgac cttgttccct tctacttttt cctgaaatcg
     9301 ggataatact tgatatttat ccgcattcat cgtgtttttt aatcagctcc gttaaattca
     9361 tcgtatcctt cggcaggttt ccagccatag gttggttcaa agtaattcca cagcagtttt
     9421 gaaatgctga taatcaactg acgcccttca ttcgtttttt tccacgacag gtctttttga
     9481 tttttggtgt aaacggcaaa aacataatca ccgtgtggtg cattgaccag caccacttct
     9541 gaccgcgatt cgctaaccat gccctgcttg gaagctgttt taacagttgg tggaatctga
     9601 cttagagctc gatcgtcgta ataaatggta cccagtatcc ggtacatttc atcgcaggca
     9661 gccggtgtga gtatttccct gttgcgtatt ttcaccaaca aacctgccat ttcgcgcggg
     9721 gtggtatgtc cccagccgta gatttcgcgg atgtcttcgc gtccgggtgt gcgggaatta
     9781 acgcgggtgt ttttcaaacc aatactgttc atcagattat taatttctgt gcctgtgccg
     9841 gctaattcct gcaaccacag actggcacta ttgtcgctgg tagtgatgct gagcatctga
     9901 acgatactca atgaaacttt cgaactgtcc tgcaacgatg caatcaggcc atcgtcaccg
     9961 gcatacagtt gttttctttt ggaataggtc aacggttgat agtaattcag ttctttattc
    10021 actattttat tgaaaattcc caccatgatg ggaattttaa tcgtgctggc cgtagggaaa
    10081 agtgtatcgg cattgtaaac ggcttcccgg cctgttttca ggtttttgac gtaaatcccg
    10141 gcaatgccgt ggaaaccgtt gacttccttt tctatcagct tggttagttt gttgtccgtt
    10201 ttctgtgcct gagcaggaag tgccaacaat acagtaaaca ggaaaaacaa tgcgcttact
    10261 cttttcattg tgaattaatt atttgtgtaa caggagagat aactttctga ttggtttttt
    10321 cttcatttac atattaacta atcataaatg tttctactca gcctcttgtt aagttataag
    10381 agtggaaata ttccatgttt tcagaaaata atatttttta taaaaaatac cttattgata
    10441 aaaaataaca atccatatgc tctgaaagtc cccctctttc tccctctcct ttggagaggg
    10501 ttggggtgag gtcgggattt agggggctgg ttagtttatt ataaatgcat caccagtaaa
    10561 ggtgtatcca cgttgaaaag cagtttggtg gcaattccct gattgaaaaa gcgtgcaaaa
    10621 atatttcgtt tcttcgtatt aagtgcaacc atatcgatgt tggcttccga gaggaattgc
    10681 tggatctggt ctaaattttc atcgcggttg aggatggcat actcggtttt tattccctcg
    10741 taatgtttac tgaaatactc ctttattcct gccagcatca cctcgtccca cgagtctttt
    10801 ttctgagagg catgtatgaa aatgagttcc agtttttcgc ggttaaagaa ggaaatggct
    10861 gtgtcgatgg cgatcagatc tttctggtca aaattagtaa ggaaagcaat acggctaatt
    10921 tcggccggtg attttaccgg taaatcaacg ggtactgcaa ataccgggac gttgcacgat
    10981 tcgagcactt ctgcggttac gcttccaatc acttcttcat cctttctgtt tttgccgcgc
    11041 gaacccataa ccaccattac cggttgttct tttttgcaca tttcgagtat ctgatcttcg
    11101 ggcacccctt ctttgagttc gaaccggaat ttaacatccg gaatttttcc ggttgctatc
    11161 catttgttta tcagattagt aaggttttcc acatctgcac tgtttgtatt cactatccgc
    11221 ctcagcattt cactgtcggc caggctgtaa gtgtttatct cattattgga tgtgattgta
    11281 aaagccggtg taaaataagc atacagaaaa atgacttcag cataaagttt ctctgcaaac
    11341 tgaaaaccat acagacatac atcgtttatc tgatcggtaa ggtcgatggg aatcagaatt
    11401 tttcgacccc tgggtggtgc atctttcgat tcttccagtt cccaggcttt ttctttttct
    11461 tccactatca ccagtgcacg tggaaggtcg ctttccttta tgcgtactct cacacctacc
    11521 gccatttcgg gatgttccag gttcaggtta tgaatctgtg tttcaatacc gctttgttcg
    11581 agtaccgttt taatcatttg ggcacgctgg taggtgcgga tggctaatgt tacaagtttg
    11641 tcttccatga ttgtagttgt ttgtgaagtt aatgatatgt ttttcagtgt tatatttatc
    11701 ctgaaatcaa cttattactc cttttctccg atggtaaaaa tagcaagaaa taatgaaaat
    11761 gatatcagat tttaagaaaa aaaacaaccg gagttaaaaa atacactata tttgcaatca
    11821 attaactctt ttcatattat tgacaacaaa ataaattgat gtatcaaatg aaaaaagcaa
    11881 tctggattat atcgctgtta gtattactgg tattatcggg tgtcgtttac tacaagtatt
    11941 attttgtatt tggcgagggt gtcaaagccg gcgaactcaa ttatatggta agaaaagggg
    12001 tggttttcaa aacctatgaa ggaaaactga tacagtcagg atttcgttct tcgggtaaaa
    12061 cggccggttc taccatacaa tcctacgaat ttgaattttc ggtggaaaat gaagggatag
    12121 ccgacaccct gatgctgagc agcggcaaag atatggagtt gcattacagg gaatacaaag
    12181 gcgtattgcc ctggcgtggt ttcagcaagt atgtagtcga taaaattata aaaatagaaa
    12241 aacaggatcc cgccaccgtt cctttttaaa tcggcttttt tctctcaaca tatattgata
    12301 attaacaatt agcaattatt tgttaacaaa aatttctgct tatttccggt aaatttgcag
    12361 gaatttatat ttacaccctt catcaaactt tattcatcat caataaactt aatacattat
    12421 ggaagtcaca gaactgaaga aatcattcaa agaagcttac ggtcacgaag ccgatgccgt
    12481 ctatttttca cccggtcgcg tcaatcttat tggcgaacac actgactaca acggaggatt
    12541 tgtattcccc tgcgccctga gtttcggaac atatttgtta atccgcaaaa acggagaaaa
    12601 attcgtacaa ttccgctcac tcaaccagcc tgaagtcgta aaagttacat ttgacaaact
    12661 aactgaacgg ttggaaaatt catgggtgaa ttatccgctc ggtgtttttg cacagtatgt
    12721 aaaacgcgga gttacattcg aagaaggttt tgatatcatg atatggggcg atgtgcccaa
    12781 cggagcggga ttatcctcat ctgctgccct cgaagtggtt accgcatatg cactgaatga
    12841 tcagctcggc acaggattga gccgtaccga actggcaaaa attggccaaa aagccgaaca
    12901 tgaatttgca ttggttaatt gcggtatcat ggaccagttt gcaagcgcaa acggtaaaaa
    12961 agatcatgca atctttttga attgtgatac actcgatttt gaactggtgc ctgtaaaact
    13021 ggaaggtgtg aaagtagtga tttcaaatac tcacagtcct cacaaattag actcgggagc
    13081 ttacaatcaa cgggttgccg aatgcaaact ggctgtggaa caactcaata aagttcgccc
    13141 aataaaatat ctggccgagc tgactgaagc cgaattcgaa aaagtacagg atgccattac
    13201 cgatccggta gctcacaaac gtgcccgcca cgtggtaggc gaagttcagc gtaccaccga
    13261 tgccgttaaa gcactgaaag ccggcgatat caccctgttc ggccaactga tgaatgcttc
    13321 acacgtttca ttgcgcgatg actatgaagt gaccggtcct gaactggata ccatggccga
    13381 agaagcctgg aaaattgatg gcgtgattgg ttcacgtatg accggtggcg gatttggagg
    13441 ttgcacagtt tcattggtga aagatgaagc tatcgaagaa ttcaaggaaa aagtgggtgc
    13501 tgcttatgaa gcaaaaatca atatcaaacc cgacttctat attgctgaaa ttggtaacgg
    13561 agccatgaaa gtggagtagt atcaaccatt ttcaacggtt actcatttat aatatcccat
    13621 tttaataata cgctctttta ccgcaatcta tcggttgaga gtacatgaaa aaatattatg
    13681 atagcaatat atacaataaa aaacgacaaa gggatgtcgg ctgaaatcac caattacggt
    13741 gcccgggttg taaaactctg ggttccagac cgtaaaggtg agcttgccga tgtggttttg
    13801 agttttgata cactcgaaga aattactacc aaagaaactt atttcaattc cacctgcgga
    13861 cgatttgcca accgtatcaa ggatggcaaa ttcacgctgg atggtaaaga atatacgctg
    13921 gctgttaaca acggacctaa tcatctgcac ggtggactgg tgggtttcaa catgcgcgtt
    13981 tgggaggtaa aatcaagttc ggaaaataaa attgtacttc attatttttc tcccgacgga
    14041 gaagaaggat atcccggaaa ccttgatgtg accgtaactt acgaattgtc ggacgaaaat
    14101 gaactcaaaa tacattacga agctacaacc gaccaaccaa cgattgtagg attaaccaac
    14161 cattcgtatt tcaatctgaa aggtgccggc gaagggcctg tacgcgacca tatcctccag
    14221 ttgaatgccg attttcatac agcactggat gacgcaacct gtccgaccgg cgaggtactg
    14281 aaagtggatg gaactccgta tgatttccgc caaccgacat taattgaaga acggattgac
    14341 gatccggtat atgcttctaa ccgcggattt gacgacaact gggtcattcg cagaaatcag
    14401 ccgcatgaac tggcgctggc gggttacatt tatgaaccga aaagcggtcg caaaatggaa
    14461 gtgctcacca cacagccggg tgtgcagatt tatgctgcca actgggtgga aaagcaaacg
    14521 ggtaaatacg gtaaagagta tgatgctcag tgtgctatct gtctcgaaac gcaaggtttt
    14581 ccggcatcac ctgcctatcc gcattttccc tcaccggtgt tacgtccggg cgaaaagtac
    14641 gacgaatggt gcatctataa attctcagcc gaataaaaaa tttttcataa gttttaatga
    14701 tggaaaacgg ttttacgctc taaaaggtgt gaagccgttt tttttaccgg tggcggtcaa
    14761 attcgcacat ttcgcctata cattggcgga tgggtgtaaa tgcaaaatcg aaggtggaga
    14821 gaaatttccg gctggaatat tctttcctgc tgatggaagt aagagccgac gaacgatcaa
    14881 gcaggggcgt gaaaccaaat attctgccca ttatttcgag caaacctgca acgggaatga
    14941 gcgatttgcc aaaccctata aacggtcgat aaacatgata tccttcggcc atccattgca
    15001 gtatatcctt gtgcgcacaa ttttcaccga ccagtacaaa ccgttcggca tttatttcac
    15061 tattcatcaa tacaatcata gcccggcaaa cgtcgcgcac atccacatag gctgtttttc
    15121 cgtaagtata aaacggcaat ccttttttca cccgtttgaa aagttcgcca cttcccgtca
    15181 ttttagccga tatgcccaaa atcactcccg gattgacgat tacagcattt aatccctgtt
    15241 cgatgccttt ttgaatttca atttctgcca gcagtttgct ttgtgcgtag gttgaaaccg
    15301 attttcccgg ttctatgggt gtattttcat caatcggtcc atgactggta tcgtccgaaa
    15361 gcgctgcaat ggaactgaca aaacagattt ttttcgcctt gcaggcaagt gcagcccgta
    15421 ccacatttcc cgttccacct acattggtct gcaacatact ttcatcgttt ttacccagac
    15481 tgaccatggc ggcgcaatga taaatataat ctgcgccttc gaatgccgtt ttcaggctgt
    15541 cagcatcgag cacatcgccc tccacccagt ccacccgacg cagatattct ccgggtttat
    15601 cggtgtaaaa ttcaaataca attttcagac attcttcgaa atcagggtgt cgtttcaaag
    15661 cagaaacccg ttcattggtc tgtaacaagt gccagattaa atgtccgccc accatgccgg
    15721 ttgctcctgt gactaaaatc attttatttc aaaattggat tatccctcaa aaatacggaa
    15781 agaaaaccgt atttttgcaa aatatttata acagtataat ccggatttaa ccatgcaaaa
    15841 tatctgtgtt tattgtgctt cgagcagtaa aattgattca aaatatttca atgccgccat
    15901 tcagacaggc aaaatactgg cacagaatga aatacgtttg atttacggag gcggttctgt
    15961 aggtgtaatg ggtgtattag ccgatagtgt gctcgatgga ggcggaaaag ttacaggtgt
    16021 aatccctcag tttatgatag atgcagcctg gcatcatgac ggactgaccg agctgataat
    16081 taccgacaat atgcacgaac ggaaagaaaa aatggccgcc cttggcgatg ctgcagtggc
    16141 actccccgga ggtgtgggca cgctggagga attgctcgaa gtcattacct ggaagcaact
    16201 tggtatctat accaaaccga ttgtaattgt gaatgttgat ggatattacg accatttgat
    16261 tgcgcaattg caacaggcgg ccgacgaact ctttatgcgt cctgatcatt tgaaaatgtg
    16321 gactgtcgtg gaatcagccg atcaaattct cgacgccata gccaatgcac atacctggaa
    16381 cgaaaatctg cgtcaatttg ccaaaattta gaatatcaga atttaatgcc ggttacagat
    16441 agcatcataa aagcagttga tacattaagc cgagttaaaa tcgattcggt tgctgtaggc
    16501 gatagtctgc tccgggccga ttcgattgca cgcatcgact ccgccaaact ggcagcttct
    16561 tcattgccaa caggaatgcc cggggtgaca catccttcat tacccatgaa tgaaccctgg
    16621 gtatttatcc ttcttataat gcttttcatc ttattggccg caagtattgt aaaatcgggc
    16681 ggtgagctgt ttcagaatat ccggtccacc tttaagcaca attacagttg gggaacatcc
    16741 ggtgtatcaa cgctcaacaa tcttcagtcg caaatattag caataacatg ctctattggc
    16801 gtgatttcat tatttgtgtt cgaacttttt tttgaaatgt ccggcaggtt tgatattaaa
    16861 acttacggaa aggttgtagg tgttactctc gcattttatt tattcaaata tctcatatta
    16921 gaggcggtag gacgtgtttt tctcaacaaa aaagccgttc agaattacct gagtttgtat
    16981 ttcaactttc tcaccctgtt tgccactgcc ctttttccgg tcttgttttt atatacgttt
    17041 caacccccga cgtggaagtt gtctctggaa cttgttgctg ctgtcctttt tggctgtttt
    17101 tatgtactgc tgattatcgc cttgtttcaa aatttttata caaaaaaatt agctctgttt
    17161 tatttttttc tatatctttg cacgctcgaa attatacctc tgttcgtttt gtttcgggtt
    17221 tatgaattaa ttgtctgatg ttttattctt ttaaactgta aagtcttgaa aattaagaag
    17281 atacttgtct ctcagcctca gccaactacc gaaaaatctc cttattacga tatcattgag
    17341 aagcataacg taaaaataga ttttcgtccg tttattaaag ttgaacctgt taccgccaaa
    17401 gaattcagag ctcagcatgt taatattctt gaatttactg ctgtaatttt caatgcccgt
    17461 cacggtatcg accatttttt tcggttgtgc gaagaaatga gaattacggt tcccgaatca
    17521 atgaaatatt tttgtgtttc agaaacagtg gcattgtact tacaaaagta cattcagtat
    17581 cgtaaaagaa aaatattttt tagcccgacg ggaaaatttc ccgatttaat ccccgtatta
    17641 aataagcatc aggaagaaaa attcctgtat atcacatccg atgtgcgcaa tgaagagact
    17701 atgtctattc ttgagaattc gaaaataaca ttcactcagg gtatcatgta ccgcactgta
    17761 agcaataact ttgcacccga tgaacccttt gagtatgata tgttgatttt tttcagtccc
    17821 gcaggtataa aatcgctcct tgaaaatttt ccgaatttcg aacagggaga tattcaaata
    17881 ggctgttttg gagccactac tgctcaaaca gtgcgtgatg ccggtttacg acttgacctc
    17941 gaagcaccat cgccaggcgt accttccatg accatgggac ttgacaattt tctgaaagaa
    18001 gctaagaaaa acaacggtaa aaaataaata tcggataaag atattcgccg tatcaataac
    18061 ataatatccc aaatgtatcg aaaaattcgt acttttggga tatctttttt ttaaccagct
    18121 gatgaataca tttcccaaaa aagaacattt atgcggtgac ataaatatca cccggcttca
    18181 tgagttgggc aacgcattta tgtctttccc tttcagggtg gtttatagga tggcggatga
    18241 caaagagtct gtgcctgtgc gggtgatggt aagtgtacca aaaaaacgct tcaaacatgc
    18301 ggtggacaga aacaggatta aaagactgat acgcgaagtt tacaggctca ataaacagga
    18361 aatttcagat tatgcaattg aatacaacct gaagcttcac atttcatttc agtatgtgag
    18421 caatgaggtg gaaaagtacg aactgctgga aaggaaaatg aaaaatgccc tgagaaaatt
    18481 gatacaaaat ctatccgtac ccaatcagac cgatgaaaat tctaactaag atattggaat
    18541 atattattct gttgcccatt tacttttatc ggtatgctat ttcgccactg aaaccaccat
    18601 cgtgcagata cactcccacg tgttcggaat atgctgtaca ggctgtaaag aaacacggtc
    18661 catttaaggg tctctggttg gccaccaggc gtatattaag ctgccatccc tggggtggaa
    18721 gtggatacga tcctgtgccc gataccttca gtttccgaaa aaaatagtat ctttgcgcat
    18781 atgatttatc ccaaacaaat tggatttaat gcatttatct gaatagttag cgtagtaacg
    18841 aataaatttt gccccttcac aaattctggt agagtaacta tacaggttgt tgagtttttt
    18901 aagaaaatgc tttattgata aaaatagata atatgttctt aaagttcccc ttggggggat
    18961 ttagggggct ggagaaataa taaatttaat ccacacgtgt gcttgaatac cacgttaaaa
    19021 aaacaagaaa atgaaaaaac aagtttccgt agcctttatg gcctgcgggt tgttattcac
    19081 aacctgcctc atcgtagcaa acatagtaga acaaaaactg atttcaatcg gaccgattga
    19141 agccaccgcc ggtttgctca tttttcccgt ttcatatatt atcaacgata tcattgccga
    19201 agtatggggt taccgaaaag cccggctgat catctggtac ggatttctga tgaattttct
    19261 gacggtgatt atttttcggt tgagtatcgt agtccccggt tccgaaaatt tttcacatca
    19321 ggaggcattt cagcttgtgc ttggcaatac tgaacgaatc agtctggcca gttttatcgc
    19381 ctttcttata ggctcatttc tcaatgcaat ggtaatgagt aaaatgaaaa tatttcagaa
    19441 aggaagaaat ttttcgctgc gggcagtggt ctccacactg gtaggcgagg gagccgattc
    19501 gctggttttt ttcaccattg catttgtcgg agttattccc aacggcgact taatgaccct
    19561 tatcctcaca cagacagcca tgaaaaccgg gtatgaaatt ctggtactgc cactcaccaa
    19621 cgtaatcgta aaatgggtaa aacgcaagga acaggaagat gtgtttgacg aggggatatc
    19681 ttacaatcca cttaaaatca atgaattgta acattatttt gacggtctgt tgctgaaacc
    19741 gttaaagaca gcataatagg gattgataag gcaaacaagc actttctcat accagttgaa
    19801 gccatttctg cgcatacctg cggttgcccg atgggataat tctcccataa ttgtacctac
    19861 aatcggggtt actatcagat cctgaatgct tggttgctcc agacctgcct cccatacata
    19921 ctcccacagt acagactgag ccagattgta aacggacgac tcaaggaagg atgaaccctg
    19981 agaacgtaag gtattgaaat aaagcgagcc ctggtacgga tgtccgatat aatttgtgat
    20041 ccacagatca ggatcaatta caggaggttt tgtgtaggca ttctgatatt tctgtttaat
    20101 catataccac ttcagccggt cgggatgttc ccatttggat atttcagaag gacccagaat
    20161 gagagcagtc atcatggcag cattctgtgc agagttcaaa agcatacccc acgccagttt
    20221 tacacccaat ggcgaatcat tatttctgta tttgaactgt ggtatagcat tactgcccga
    20281 tgattccgta cttttcttaa tcgtttcagt agaaaaaacg gtgacggtat cgtctgggga
    20341 taaagttaag gaatcggggg tggtctgtgc cctgataaca gcaataatac ataaaaagaa
    20401 aataatgaag gaatgctttc tcatgatgca ggtaaatttc ttaaaaaaaa tattctaaaa
    20461 aatcagataa aagtgagcag cgcattttca gaattctgaa atagaagcca tattcactaa
    20521 taaaaagata aaaggtaaca gaacggaatt tatattcgaa aatcacatac cagaggatgc
    20581 tccaaagtta caaaatcata aattaaatca aacaacattt acggattaaa tgaaaaaatg
    20641 gacagacaat cggctattta cacctctttg ttctgtccat tttatgattt tttctcgaaa
    20701 atctgaattt tgcgcttcga atggttattg aagaattaat ttttttgtaa aaaatttgtt
    20761 gcctgtgtta attcttatta cgtaaatgcc tttactgtaa tgagataagt caaacgattt
    20821 aagctgattg ccgatttttg aatcgtccag aaccatttca cccactgaat tgaaaatctc
    20881 aattccgtca atattttctg acgatgacac gacgaatttt cctttacccg gattggggta
    20941 aacagtgatt tgatcgtcgg acagattgta aacaccggtt gcaggtcggt aatagtagcg
    21001 ggtgctgtcg ccgttactaa cggtaatgcc attttcgagg aataatttgc tggaataggt
    21061 tatcggataa ttgttggagt cataggtttc cacatcgagt gcagaccggt accaggcatt
    21121 gttgagccac gtctgatatt ctgtgcggta attatttcca tttccatcgt atgtgcgggt
    21181 ttccagcaca tcattcaccc agtcatttgc cccccagttt tgatgaagat cagtcagcaa
    21241 ttcgtcggta gcgctgtagg tatagctgtt tttggaatta ttgacccagg ctccgttttc
    21301 ccagatttgc cgaatactac taatttcgtt ctgtcgggtg tcataggtcg aaatccattg
    21361 agattgattt ttccagcctg aattccagat ttgatttaga atgttggtaa tgttctcctt
    21421 tgtgtcgtaa gtatatgtac gtttatcagt gttaagccat gccccggcat cccatacctg
    21481 attggtgatg gttagtacat ttcctttact ggtgtaagta ttcacggtat aagtgctgtt
    21541 tacccactgg tcggttaccc atgtttgctt gagagaggtc agtttgtgat ttgttgcgtt
    21601 gtaggtgtat atctcttttg aatcattgac aagagttcct gttatgccgg attgagtaat
    21661 ttgggtcaat aaattgttat tggcatcgta ggtcatggtg gtgaaggtaa atgtcatcca
    21721 catggtcata aaaatcattt ctgttttaga ggtaatcatg tttttattgg catcgtaggt
    21781 catggtggag cgcatggagt ttacccattt ttcaccgtcc cagctctccc agaggactga
    21841 ggtcatcaca tcgttactgt tataagtgta ggtgtatttt atttcattta cccactcacc
    21901 agcatcaatg tcccattgct gatttattgc actgacaagc ctgtttttca tatcgtacgt
    21961 catcatgatg gttttttcgt cattcaccca gttaccgata tcggaattcc agtcccaata
    22021 tcgcatactg tcaagtaatt caggctgaac tgcctgtgcc tgagctgcct ttattatttc
    22081 gtcaattttt tgggtggtga gcactgcact tgtcttcagt gactgaagat cccgaataga
    22141 acgggcaata ttcagtgaac ccgggatagt ctgtttttta atatcctgat ggaatggatg
    22201 atgttggcca gcaacaggga tagagaaaac gaaaagagca aacagagtaa acggtaaaaa
    22261 ttttttcatg attgttggtt tttaaagtta atactctata aataacaaaa aatcgtgaaa
    22321 aatgttttaa ttcatttgtt atttaaatga attttaaact attgatatcc tgtctgttga
    22381 caatgattgt tttactctgt tcagaaaatt tcgttcagca aattggccag ctgaattcct
    22441 ccgcggtaca gcatttcatc caggtcatca ttgaactgat acatataggt gtaagctctc
    22501 agattggtgt aattgtatgc atatattttg ttgcggacca gataaacggc ttccaccgat
    22561 tgtggcagcg attgttgttt aaattctttt tttcggggtg caaatttatc ctgcagataa
    22621 gctgtgtatt cggagtagga aaaattgtga ccttcaatca tctgtgtatc ccacagttgg
    22681 tgaaggcgga tgtctttgcc aaaccatttg atcatcacat catttccacc gcggtcttcg
    22741 gcacgtccca gatgcatggg ctgatgggca tcggctatga aatgtataag aaatttgagg
    22801 gcttccaggt cttttttgtt ttttttcaga ctgccactca ggctgtcgat ggcaaaaaac
    22861 agatggcgtc cttcggcagt cgggttttgg aaaagggtct gaatatcagc cggtgtcatg
    22921 ccttcgcgca gattttgaaa atgccagtcg tagctgtagg cgtaagccga atcacttcga
    22981 atatcgtcgc tccaggtgga ttcgtaggtt aatccgtgtt ttcccagcag cttatccact
    23041 ttatggcggg tatgacaagt cagattttgg taagctatgg tggctactat ccggtgtccg
    23101 aatgcatcgt aagccacagc gggcagtgtg agaccaacaa caaagacgag aagaagaaat
    23161 tttgctttca tacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttcac
    23281 tacttgacga atatcacttc tacctgaaaa tggagaaatc gctatcagaa aataccatcg
    23341 aagggtatga gcgagatttg cagaaactgt ttgattatct ggcagaggcg cacattcagc
    23401 cccaggatgc tgagaatagg catttgcgtg attttatcat cgaaattgcc gaaatcggta
    23461 ttaatgaacg atcgcaggca aggctgatat caagtatccg ttctttttat aaattcctga
    23521 tttacaaaga caaaatcact gttgacccga ccgaattgct ccaaagtccg aaaatcgggt
    23581 tgcgattgcc agaagttctc acattatccg aaattgaccg aattatcgaa aacatcaatc
    23641 tatccaaacc cgaaggacaa cgcaataaag caattatcga ggtactttac ggctcgggat
    23701 tgcgggtatc agagctgatc agtctcaatc tttcaaacat ctatttcgac gaaggatata
    23761 tgctcgtgtt gggcaaggga aataaacagc ggcttgtgcc gctttcggat gaatcaaaaa
    23821 agcagattgg attttggatt atggaccgta atcatctgaa tataaaaaag gggaacgaag
    23881 attttctgtt tcttaatcgt cgtggaagca aacttacacg tgcaatgatt tttaccatta
    23941 tcaaagaact tgcagtgatg gccggaattc ataaaaatgt gagtccccac acattccgtc
    24001 actcatttgc cacacatctg ctcgaaaacg gtgctaatct gagggcaata cagcagctgc
    24061 tggggcatga atccatcacc actaccgaac tttacacaca tatagatgtt caatttttac
    24121 gacaaacaat tttggaatgc catccgatga atcaaaaaaa caaaaaacat gaagttgaat
    24181 aattcgtttc tgcaccgtat cacgaagcga attcttcttt ccggtgtggc ttttctattc
    24241 ttgtcaactg gcacgctggc gcaggaaaca gtggtggtgg ggcaggtgct ggataagtac
    24301 gacaaatcgc ccatatccac ggttgatatt tatttcaaaa actccaaccg ggctgttcaa
    24361 agcaacgatg atggatattt tctcatacgc aacaacggcc ctgaaaccac cctcattttt
    24421 accatagtag gctataaacc ctatgagttg aaactcaaac gcggagataa cgtgggagtt
    24481 cagatagagt tggaggagaa aaaaaatatt ctcgatgaag tgcttgttct gcccggcatt
    24541 aatcc
//
LOCUS       DAOPZK010000014        23338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2199_length_23338_cov_22.367908, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000014 DAOPZK010000000
VERSION     DAOPZK010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 23338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23338
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_23338_cov_22.367908"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKH68_01700"
     CDS             complement(<1..739)
                     /locus_tag="PKH68_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11677.1"
                     /translation="MNYKKSLYLLLLCVIFSSFSGSIAAQSTPKYELYGFIRNDFYFN
                     SRQNVEVIDGIFHMFPKPNVPDANGKDINNVAQAEMISVASRLGLNVNGPDLFNAKST
                     AKIEFDFCGTGSTYFLIRLRQAYMKLNWKKTELLAGQTWHPLYGNVAPTIVSLNSGSP
                     FQPFNRSPQLRVKQNLTSNLSITLAGIYQMQYTSYGPEGKSNIYMKKALLPDLYFGLE
                     NAGKHLTGGIGLDVKTIKADSRLLSKSV"
     gene            complement(773..1342)
                     /locus_tag="PKH68_01705"
     CDS             complement(773..1342)
                     /locus_tag="PKH68_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNX11678.1"
                     /translation="MDFISILIISIGLAMDCFAVSISKGICTGRFSFVNTLKMALLFG
                     FFQGLMPLIGFFAGKTFIHELSTYDHWVAFALLGIIGGKMLVEGLKPVDPTCETTTNP
                     FKFSTLISLAFATSIDALATGIVFVPFPEKIYYAVSIIGVVSFLFSFLGMAIGIHFGR
                     RFKFNVEILGGIILIGIGTKILIEHLFLN"
     gene            complement(1417..3438)
                     /locus_tag="PKH68_01710"
     CDS             complement(1417..3438)
                     /locus_tag="PKH68_01710"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNX11679.1"
                     /translation="MHTTQDLNRAADNIRILAAATVEKAKSGHPGGAMGGADFINVLY
                     SEFLEYDPENPYWEGRDRFFLDPGHMSAMLYSVLALTGKFSLEDLQQFRQWGSVTPGH
                     PEYDLSRGIENTSGPLGQGHTYAVGAAIAAKFLKARFGEVMNQTIYAYISDGAVQEEI
                     SQGAGRIAGHLGLNNLIMFYDSNNVQLSTYTDSVTSENVALKYQAWGWKVIEINGNDA
                     TEIKVALREAKNEKEKPTIIIGKTIMGKGAVAADGNSFENEIETHGQPLGASGASFAD
                     TVKNLGGNPDNPFVIFEETKAIYAIREAQLKELMAEKKQAKKEWETKNPELAEKLIQF
                     FSGKTPEVNWDAIEQKPNQATRAASATVLTYLAQHVENMIVSSADLSNSDKTDGFLKK
                     TTAFTKNDFSGKFLQAGVSELTMACICNGMALHGGVIPACATFFVFSDYMKPAVRMAA
                     LMQLPVKYIWSHDAFRVGEDGPTHEPVEQEAQLRLMEKLKNHHGQNSMLVLRPADVEE
                     TTVTWKMALENTKSPTALILSRQNITDLPAANRKEEAKKAEMGAYVVESDENPDVILL
                     ASGSEVATLAESAALLRADGIKICLVSVPSEGLFRSQSAEYQEAVLPKAIKKFGLTAG
                     LPVNLLGLVGENGKIWGLESFGYSAPYKVLDEKLGFTAENIYNQVKSLF"
     gene            complement(3469..4191)
                     /locus_tag="PKH68_01715"
     CDS             complement(3469..4191)
                     /locus_tag="PKH68_01715"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HNX11680.1"
                     /translation="MSKTLKYLLLSSLLVFSAIATAQQSTTNATKSKTYIQEKKDTLS
                     VPLWQGFMVEFDVEPLIENVILNNSTYTAYTYQGNIQGNLKNAYFPVVELGLGGASKS
                     LTNSHSYQGDGMYGKLGVDFNLLKPKPGTKIIKNYFLAGARLGASHLNYTIDNLTIAN
                     DYWGGTEDINYSRATTKVWFEVVAGVRVSFFKNIYLGWSVRNKHLLNQGKYGSPKPWY
                     IPGYGIGNSSAWGFSYVVGYHF"
     gene            complement(4157..4648)
                     /locus_tag="PKH68_01720"
     CDS             complement(4157..4648)
                     /locus_tag="PKH68_01720"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HNX11681.1"
                     /translation="MRKFNLWLILALLFGIGWSSCSTEETCRKSKTVLLGAKLYQDTL
                     NTKTNQYVTLSYKADSLTSLGVGVNMYIYKSAKDLSEIKLPLDKLQTQSRFSIKFNEI
                     YDTITVVYHNTDEYMSLECGCVRTHTIDTVLTTNHIIEKIQLSKPIINTTYVENIKIF
                     IAK"
     gene            complement(4657..5172)
                     /locus_tag="PKH68_01725"
     CDS             complement(4657..5172)
                     /locus_tag="PKH68_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11682.1"
                     /translation="MWTEILKYTLPAVLVIVVTYLLLHKLFKNEGERRQYELKKINLS
                     TITPVRLKAYERLMLLLERINPNRFLISKVEADMNSLELHGTLLREIRREYEHNLSQQ
                     IYVSNELWESITNAYENLIQLINTCAAQCPAEAPASKLATMIITVYESVEETALDAAR
                     QALKDEVKEIF"
     gene            complement(5178..5528)
                     /locus_tag="PKH68_01730"
     CDS             complement(5178..5528)
                     /locus_tag="PKH68_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNX11683.1"
                     /translation="MESEYSILKVYVSNTDKYGMSLFYEHLVMEAFKNGIAGVTAYRG
                     ITGYGLSSKTISTSKFWEINEKLPVMVEMIDRTEVLEAFFEHIEKDVKTMGKGCLIYM
                     QPIDVKIHQSGRKR"
     gene            complement(5531..5905)
                     /gene="crcB"
                     /locus_tag="PKH68_01735"
     CDS             complement(5531..5905)
                     /gene="crcB"
                     /locus_tag="PKH68_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008564572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNX11684.1"
                     /translation="MRQLLLVGFGGFLGSIARYLVAKLNLTWNFHGIPMGTLTVNILG
                     SILIGFFLGIFVNSDLLNTNLKLFLVVGFCGGFTTFSSFTNENFILLQNGQFLTAFVY
                     ILGSVLVGILSVYIGYLISNLL"
     gene            complement(5961..6911)
                     /locus_tag="PKH68_01740"
     CDS             complement(5961..6911)
                     /locus_tag="PKH68_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX11685.1"
                     /translation="MDISVIVPLYNEEGSLTKLFEWIDKVMTENKFSYEVIFINDGST
                     DGSWKVIEDLKAQSPNVSGIKFRHNYGKSPALYCGFRAAKGDVVITMDADLQDSPDEI
                     PELYSMIMKEDYDLVSGWKKKRYDAKLTKNLPSKLYNATARTVTGLKLHDMNCGLKAY
                     RHDVVKNIEVYGEMHRYIPYLAKNAGFTKIGEKVVEHRKREFGSSKFGMNRFVNGYLD
                     LITLWFLSKFGKQPMHFFGLIGSLMFILGLLAVIVVGAKKLIALSSHIASPLVTSSPY
                     FYIALTAMILGTLLFLTGFLAELVTRNAPERNNYKIEKEI"
     gene            complement(6946..7476)
                     /locus_tag="PKH68_01745"
     CDS             complement(6946..7476)
                     /locus_tag="PKH68_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNX11686.1"
                     /translation="MQTNVLNAAMKYGLIMGLIFSVNFLLSVTGNPIISLLGYLVVAV
                     ILVLTYRFSVAYRDKENGGTITFGHAFFFILLLFLFAAIISSLIKFIYFKYLNVEYLP
                     QLLEQSITVMNQMQEQFHFNIPDDYYQNMENMMNPATYTLQFIWVNVILGVFVGLIMA
                     PFIKKNKDIFDNTDAN"
     gene            complement(7644..8294)
                     /gene="nth"
                     /locus_tag="PKH68_01750"
     CDS             complement(7644..8294)
                     /gene="nth"
                     /locus_tag="PKH68_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNX11687.1"
                     /translation="MTKKQRFQNIIAWFEENRPVAETELLYKDAYSLLVAVILSAQCT
                     DKRVNMVTPKLLNDYPAPEALAASTPEVIFEYIRSVSYPNNKAKHLVGMAQKLIRDFG
                     GEVPDEVDKLVTLPGVGRKTANVIASVVFNKPAMAVDTHVFRVSERLGLTTNSKSPLE
                     TERELVKHIPDELIPKAHHWLILHGRYVCLARKPKCEECGLKQWCRYFEKNNLPKK"
     gene            8424..10874
                     /locus_tag="PKH68_01755"
     CDS             8424..10874
                     /locus_tag="PKH68_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNX11688.1"
                     /translation="MPPKKPVKKNIAEKQPVNRSKPKSTEPGLFTKIIHFIKDEKLRF
                     ILGILLALAVLYALLSFVSYFFTGAADKSVFDNISFRDSLQIRSSVQNWTSVMGAYIS
                     ETLIDNWFGVSAFTILIFLFIAALRLMKVKLVSIWKAFFHLFFWLIWTSVTLGYLTDF
                     LPSLQPSFFALGGQHGTFTATELNSYVGRPGALLIILASLLIYMIVVWSATIPMIRNL
                     FHIKDKTPAYDENDEPIVAVGTEIVPEEWLAAEALKYKTVVDDTDKNEEFTDDVEDPN
                     DTVGFEVTNPHKEETENPVEEYEEEENSDPNYNISKLGKYDPTLDLSSYNPPTLDLLK
                     VYGSDNDMQIDMKEQTANKNRIISTLRNFGIEIDSIKATVGPTITLYEIVPKSGIRIS
                     KIRNLESDIMLSLAATGIRIIAPIPGKGTIGIEVPNNDPQIVSMNSVIGSKRFTEAKF
                     ELPIAFGKTITNEVFMVDLTKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPSQLKFV
                     LIDPKKVEFNIYAAIEKHFLAKLPDGEDAIITDTSKVVQTLNSLCMEMDDRYDLLKKA
                     HVRNIKEYNEKFVNRELNPEKGHRFLPYIVVIVDEFGDLIMTAGKEVEMPIARIAQLA
                     RAVGIHMIIATQRPSVNIITGVIKANFPARVAFRVSSMIDSRTILDAPGANQLVGRGD
                     MLFSQGSELIRVQCAFVDTPEVEQIALYIGNQRGYPTAFYLPEYVGPESEGIDTSSVD
                     MSKRDPMFEEAARLIVASQQGSTSMIQRKFSIGYNRAGRIADQLEVAGIIGPNEGSKA
                     RQVLVQTEYELENILKHL"
     gene            11023..11667
                     /locus_tag="PKH68_01760"
     CDS             11023..11667
                     /locus_tag="PKH68_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNX11689.1"
                     /translation="MKTKKIVYTLLMALFIFPATYAQSSANAVKVVNDMMNLLKTNAI
                     STNFVMTVKEPTSTQAQSIKGNFTMKENKFILNTDEMNVYFDGKTQSAYMTSVNEVSI
                     TNPTEKELAETNPLAVLSAYKNKSIIKFSKKSQSKTVHAIELTPKDQNSTIKKILVNV
                     NKSNNYPLFVQLTDRKGAISTLTLSQFKTGLKILDSSFVFNPKKYKDIEINDLR"
     gene            11788..12729
                     /gene="trxB"
                     /locus_tag="PKH68_01765"
     CDS             11788..12729
                     /gene="trxB"
                     /locus_tag="PKH68_01765"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNX11690.1"
                     /translation="MNEKVRCLIIGSGPAGYTAAIYASRANLAPVLYEGMQPGGQLTT
                     TTEVENFPGYPEGITGPELMEDLKKQAVRFGADIRFGMVSATDLSATPYKVTIDGEKV
                     IEAQTIIISTGATAKYLGLPDETKYAGSGVSACATCDGFFYRKRKVAVVGGGDTAAEE
                     AAYLANIADKVYLIVRKSYLRASKIMQERVMTNPKIEILFEHETLGLTGDKVVEGVDL
                     VKRRGQPDEEKVHIEVDGFFLAIGHTPNSGIFKPWIETDEVGYIKTIPGTPKTNIEGI
                     FAAGDVADPTYKQAITAAGTGCQAAIEAERYLSEKGL"
     gene            complement(12795..13046)
                     /locus_tag="PKH68_01770"
     CDS             complement(12795..13046)
                     /locus_tag="PKH68_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNX11691.1"
                     /translation="MTKTVTFNELRRIKDALPSGSMAKIAEELGLEKQTVKNYFGGSD
                     YKEGKSVGLHIEPGPDGGIVQLDDTSILEVALRMLEEIK"
     gene            complement(13202..14566)
                     /locus_tag="PKH68_01775"
     CDS             complement(13202..14566)
                     /locus_tag="PKH68_01775"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNX11692.1"
                     /translation="MEIKSYIRDHETRFLNELFSLIRIPSISSHASRQPDIHRCAERW
                     KQLLEEAGVDIAEILPTSGNPVVFASKLIDPKLPTVMVYGHYDVMPAEPLDLWDTPPF
                     EPEIRDGKIFARGADDDKGQSFMHAKAFEYLVRTNQLKCNVKFILEGEEEIGSPSLEE
                     FCKNNLKKLKCDTILVSDTSMLSAHTPSITTGLRGLAYWEIEVTAANRDLHSGIFGGA
                     VANPILELTKILAQLKDKDGRITVPQFYDDVAGVTKSERELIAQIPFDLEEYKSNLGI
                     KQVFGEKGYSTIERTGIRPALDICGIWGGYTGEGSKTVLPSKAYAKLSARLVPHQKYE
                     KIARLVEKHILKIAPKYVDVKVTQLHGAESYVCPIESEEYKAAENAYTKVFGVRPLPV
                     RRGGSIGVVPVFEKILKVKPILMGFGLESDAIHSPNENFPLDLFEKGIETIVEFYNQY
                     SEKR"
     gene            14856..15647
                     /locus_tag="PKH68_01780"
     CDS             14856..15647
                     /locus_tag="PKH68_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX11693.1"
                     /translation="MKKINIILIVSTLLIFTGCTTQKKLAYFNTVTASSAESINAQLK
                     NERADARILTDDRLSITVSALDPNAVAIYNLPFVSFASPGSDQIYASPVLQSYLVNSQ
                     GNINFPVLGEIKLEGLTLTEAGNLIKNKLAEHVADPIVNIQFVNFRITVLGEVLRPGQ
                     FPVTNERVTILDALGLAGDMTAYGRRDNVLLTRNNNGKLEFARINLNSDEVFKSPYFY
                     LQQNDVIYVEPNTVKSISSQDIPLYLSSLSTLATLVTLIYSISKK"
     gene            15697..18099
                     /locus_tag="PKH68_01785"
     CDS             15697..18099
                     /locus_tag="PKH68_01785"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX11694.1"
                     /translation="MEKNNNNINNSINNTEVINLRELLTRYVQKWYWFVLAVIIAFIV
                     ATIYLRKTDNKYQVQTVILLRNEDNSSQISQLAMMESFGLMGSSRVVDDEIQVINSNK
                     IINQVIDTLGIEVEYFEKTGLKYVEKYHTLPFKLNLVNHFADSLKAPIRITLKTGTGS
                     VKVSVKYGRDWKNTYTLDNIQQPFSTPAGVLSLKSLSSVKPGMRYKINVYPKKDLLAQ
                     YRGKLNVSVVNKQSNAIRISIVEFNIKKAEDILNKIVELYNMDAIIDKNIVAANTGNF
                     IEDRLRLITKELFDVESEVESYKKANSLTDIASEAGIYLASASEYEKKIAELETQLNL
                     IQYIESYIRDAKNQYSLIPANIGVEDKSLMELILNYNAIVLERMKLSQSANEKNPVLI
                     QIEQQIKALRGNVLESIGSVKSGLKIAKNDVTRKDAQFNSRIKAVPTQERQFLEIKRQ
                     QEIKQNLYLFLAQKREENALSLASTAPAARTIDRAYPSLTPVSPKRMIIYLVALILGL
                     LFPFLYIYLRDLINNKIEDAKEFQKTVNPPFLGSICISRESDRVVVQAGKTTPIVEMF
                     RLVRTNLQFIIGSKKNPAILITSSISGEGKSFISINTAMSLALLNKKVVLIGMDIRNP
                     VLRDYMHLSNVNSNGVTLYLSDTDCQIGDIIMPSGYNNNLDVIPAGPVPPNPSELLLS
                     PRLDELVLELKKIYDYIIIDSAPIGIVSDTYLLNRVIDNCIYVARQDHTPREASNLIN
                     EIYAEKRLNGMALILNGTSATSSYGYGYGYERNKNKFKNMPKLTFGDKLNNAILKLFK
                     RN"
     gene            18317..18928
                     /locus_tag="PKH68_01790"
     CDS             18317..18928
                     /locus_tag="PKH68_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX11695.1"
                     /translation="MDTVAENIIHTSHQKFKNFGIRSISVDDICNELHISKKTFYSRF
                     PQKECLIEAVLDYESEVSTDNFEKTYKNKNAIDALIIIIKDLKNSIDKAPLTFHYDLE
                     KYYPATFTKYKERQSTRIRNCFEINLRKGIEEGYYREDIDVELVSLFHSIQIRETFEQ
                     MQKASPKITKKQLVDFFIDLIVRLITNENGYKYMNENYYKLKD"
     gene            18949..20292
                     /locus_tag="PKH68_01795"
     CDS             18949..20292
                     /locus_tag="PKH68_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX11696.1"
                     /translation="MKKTGLFILGLMLAGNIAAQDTIVLSLPQALDIALSENPTIRIA
                     NKEIKRVEYNKKEKYGALLPNISLGLSYARTLKKQKMFFSIPGMPANPDGIEVGQDNT
                     FNGSTQGLVASLPLYAPALWQSINMSELDMELALESARSSKISLVNQVSKAYYTILMA
                     QDSYKVLMRTFTNTTENNRIVQNKFKQGVVSEFESIRAEVQLRNVAANLSAAENAVEL
                     TKLQLKMLMGMDIDQQIKVEGNLSDYENKMYADVLKIDTTTLASNSDLKQFDIKTRQL
                     NQSSKLQRASWMPTLAASFNYNYMSYANDDIIFTGDHRWFPTSNIGLVLSIPLFQGGQ
                     RYFKDKQMQIQIDELKDQKLNLKRGLELQAMTYINNMQKAIKLIESNKVALSQAEKAM
                     AISQKRYEVGAGTYLDVANAELAYQQSGLSYNQSVFDYLSAKADLEKLLGNEKTN"
     gene            20319..21338
                     /locus_tag="PKH68_01800"
     CDS             20319..21338
                     /locus_tag="PKH68_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX11697.1"
                     /translation="MKNFKLITISLLAIALSSTSCTKSDKTKTQSSDAKIPVKIAQVS
                     EKNVPQIVEYPATVQPVFKNNIAPSAPGRIRRIMVDVGSRVGAGQRIVQMDAANLANY
                     QTQIDNLRRNYNRVSELLSVGGASQQDVDNVKVQLEQAENTLKNLNENTYLTSPISGI
                     VTARNYDNGDMYSGQLPVLTVMQINPVKILINVSESYYSKIKVGMGVDMSFDVFPNER
                     FNGNVSLIYPTIDERTRTFTVEIKLPNNNSKVRPGMFGRVIVNYGYANRVVVPDLSVI
                     KQAGSGARYVFVYNDGKVTYKQIEMGQRIGNEYEILSGLSTGDQVVVAGQSKLVDGSM
                     VEIIK"
     gene            21435..>23338
                     /locus_tag="PKH68_01805"
     CDS             21435..>23338
                     /locus_tag="PKH68_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNX11698.1"
                     /translation="MSLYESSVKKPVMTSIIFIAVVILGIFSYNKLPIDFLPKIETNT
                     ILVMTTYSGASASDVETNVTRPLESSLNTVSNLKNITSVSKENTSIITMEFDYGLDLN
                     VVTNDVRNKLDIVKSIMPAGAGSPIIFKLSTDMIPVMVITATADQSLPALYKILDDKV
                     GNPLARINGVGSVSISGAPQRQIQVFVDPVKMEAYHLTVEALAQYIRMENVNTPAGSM
                     DVGSDTYALRVQGEFKDPSELNNIVVGNYNGKTVLLSDVAVISDKHEERMQESFTNGT
                     QGASIVVQKQSGANTVQIATEVKKNLPDIQKTLPSDIKLNVIMDTSENILRTVSSLEE
                     TVLFALLFVMLVVLFFLGRWRATVIIILTIPISLISAFIYLYLTGGTLNIISLSSLSL
                     AIGLVVDDAIVVLENITTHIERGGRPKPAAINGTNEVGLSIVASTLTIIAVFLPLTMV
                     SGMAGVMFAQLGWMVTIIITISMICALTLTPMLSSQLLRLNPNRSKGFNMIYGPIERL
                     LDNLDKKYAQIVNWAVRHKKSVMLFATLFFFISVTPMIVGWVGTENFPAQDSGIISTT
                     IELPVGTRMELTRDVCQKLNDEWMKKYPEIRMLNYNVGQASSKNVFGSLFGKNGSNIG
                     SFSIKLVELE"
BASE COUNT         7132 a         4708 c         4650 g         6848 t
ORIGIN      
        1 ttacactttt cgataaaagt ctgctatctg ctttaatcgt tttcacatca agcccaattc
       61 ctccggtcag atgtttaccg gcattctcca ggccaaaata caaatccggc agcaacgctt
      121 ttttcatata tatgttcgat ttgccttcag gaccgtagga tgtatattgc atctgataaa
      181 taccggcaag cgtaatggat aggtttgaag ttaggttttg tttaactctc aattgtggac
      241 tgcgattgaa tggttgaaaa ggagagccgg agtttaacga aacaattgta ggggcaacat
      301 ttccatagag cggatgccag gtttgacctg caagtaattc ggtttttttc cagttcaatt
      361 tcatatatgc ctgtcgtaac cgtattaaaa aataagtcga gcctgtgccg caaaaatcaa
      421 attcaatttt ggctgttgat tttgcattga agagatccgg accattgaca tttaatccca
      481 gacggctggc cacacttatc atttcggcct gagcaacatt attaatgtct tttccgttgg
      541 catcgggaac attcggtttt gggaacatgt ggaaaattcc gtcaataact tccacatttt
      601 gtctggaatt gaaataaaaa tcatttctta taaaaccgta aagttcgtat tttggagtgc
      661 tttgagcagc tatactaccc gaaaatgaag aaaaaataac acaaagaagt aataaataaa
      721 gtgatttttt ataattcata gctggttgat gggagtaaga gatatcgtat aattaattta
      781 aaaacaggtg ttcaatcagt atttttgtac caataccaat cagaataatt ccacccagta
      841 tttccacatt aaatttgaaa cgccttccga agtgaatacc aatagccatt cccagaaagc
      901 tgaacagaaa actcactaca ccaatgatgc tgacggcata ataaattttt tcggggaacg
      961 gaacaaatac aatacctgta gcaagtgcgt cgatactggt ggcaaatgca agtgaaatta
     1021 atgtgctgaa tttgaaagga ttggtagttg tttcacacgt tggatccacc ggtttcaaac
     1081 cttcaaccaa catttttcca ccaatgatac ccaatagtgc aaacgccacc cagtgatcgt
     1141 aagtgctcag ttcgtgaata aaagtttttc cggcaaaaaa tccaatcaat ggcatcagtc
     1201 cctgaaagaa accgaacaat aatgccattt tcaacgtatt aacaaacgaa aacctgccgg
     1261 tacaaattcc tttgctgatt gagaccgcaa aacaatccat tgccagcccg atgctgatga
     1321 tcagtattga aataaaatcc atttgtattt aattaaaaat atgccaatga gccaatcctg
     1381 gattgacatc atcagcatat tttgtttttt attgaattaa aataatgatt ttacctgatt
     1441 gtaaatgttc tcagcggtga aaccaagttt ttcgtcgaga accttataag gtgcagagta
     1501 tccaaacgat tccaatcccc agatttttcc attttcacct accaacccaa gcaaattaac
     1561 aggaagtccc gccgtaagtc caaatttctt aatggctttg ggtaatacag cttcctgata
     1621 ttcggctgat tgtgaacgga aaagaccctc tgaaggtaca gaaaccagac aaatcttaat
     1681 gccgtcagca cgaagtaatg cagcactttc ggcaagtgta gcaacttctg aaccggatgc
     1741 aagtaaaatt acatcgggat tttcatcact ttctaccaca taagcaccca tttcggcttt
     1801 ttttgcttct tctttgcggt tagcggcagg cagatcggtg atattctgac gcgaaaggat
     1861 gagcgccgtt ggacttttcg tattttccag tgccattttc cacgttacgg tagtttcctc
     1921 cacatcagcc gggcgcaaaa cgagcatcga attttgtcca tgatgatttt tcaatttttc
     1981 catcagtcgg agctgagctt cctgttctac aggttcgtgg gtcggaccgt cctcaccaac
     2041 ccggaatgca tcgtgcgacc agatgtattt caccggcaat tgcatcagtg cagccatacg
     2101 aacggcaggt ttcatgtagt cggagaatac gaagaaagtg gcgcaggcgg gaatgacacc
     2161 accatgaagt gccataccgt tacagatgca ggccatggtc agttcagata caccggcttg
     2221 cagaaatttt cctgaaaaat catttttcgt aaatgccgtg gtttttttta gaaatccgtc
     2281 tgttttatcg gagttgctca ggtcggcact cgaaacgatc atattttcca catgctgagc
     2341 cagataagtg agaacggttg ccgaggcagc ccgggtagcc tgatttggtt tttgttcgat
     2401 ggcatcccag tttacttcag gagttttacc cgaaaagaac tgaatcagtt tttcggctaa
     2461 ttcgggattt ttggtttccc attctttttt agcctgcttt ttttcagcca tcaattcttt
     2521 caactgtgcc tcacggattg cgtagatagc ttttgtttct tcgaaaatta caaaaggatt
     2581 atcggggttg ccacccagat tttttacggt gtcggcaaag gatgcaccac tggctcccag
     2641 cggctgaccg tgtgtttcaa tttcattttc gaatgaatta ccatcggcag caacagctcc
     2701 tttgcccatg atggttttac caataataat ggtcggtttt tccttctcat ttttagcttc
     2761 acgcagtgcc actttgattt ctgtggcatc gttgccgtta atttcaatga ctttccatcc
     2821 ccaggcctga tatttaaggg ctacattttc gcttgttacc gaatcggtgt atgttgataa
     2881 ctgaacattg tttgaatcat aaaacattat caggttgttc agtcccaggt gaccggcaat
     2941 acgtcctgca ccctgtgaga tttcctcctg aacagctcca tccgaaatgt atgcataaat
     3001 ggtttggttc atcacttcac caaaacgtgc tttgagaaat ttggctgcaa tagctgcacc
     3061 tactgcgtat gtatgtccct gacccagcgg acctgaggtg ttttctatac cgcgggagag
     3121 gtcatattcc ggatgcccgg gtgtgacact tccccattga cgaaattgtt gcaggtcttc
     3181 caaactgaat ttgccggtga gtgccaatac ggaataaagc atggccgaca tgtgtcccgg
     3241 atccagaaag aagcggtcac gaccttccca gtaaggattt tcgggatcgt actccagaaa
     3301 ttcggaatac aatacgttga taaaatcagc tccacccatt gcaccgccgg gatgtcccga
     3361 ttttgctttt tcgactgtgg cagctgccag aatgcgaata ttatccgcag cgcgattcaa
     3421 atcttgtgtt gtgtgcataa taactatagg tttaatgtaa tgaatttatt aaaaatgata
     3481 tcccaccacg tagctaaagc cccacgccga tgagttgcca atgccgtaac cgggtatata
     3541 ccagggtttt ggagaaccgt attttccctg atttagcagg tgtttattgc gtacgctcca
     3601 gcccagataa atgtttttga agaaactaac ccgcactccg gcaactactt cgaaccagac
     3661 tttggtcgtc gccctggaat aatttatatc ttcggttcct ccccagtaat cgttagcgat
     3721 agtcaggttg tcgattgtgt agtttaagtg agatgcacca agtcttgcac cagccagaaa
     3781 gtagttttta atgattttgg ttcccggttt tggctttagt aaattgaaat cgactcctaa
     3841 tttcccatac attccatcgc cctgatagct gtggctattt gtcaaagatt tggaagcacc
     3901 tcccaatccc agttccacta ccggaaaata agcgtttttc aggtttccct gtatatttcc
     3961 ctgataggtg taggctgtat aggttgaatt attcagaatg acattttcta tcagaggttc
     4021 aacgtcgaac tccaccataa aaccctgcca gagaggtaca cttaacgtat ctttcttctc
     4081 ctgaatgtac gtttttgatt ttgtcgcgtt tgtcgttgat tgttgggctg tggctatcgc
     4141 actgaaaacc aatagactac ttagcaataa atattttaat gttttcgaca taagtagtgt
     4201 tgatgattgg tttggatagt tgaatttttt cgatgatatg attggtagtc agtaccgtat
     4261 caatagtgtg tgtacgcaca catccgcatt cgagcgacat atattcatct gtattgtggt
     4321 acacaaccgt tattgtgtca tatatttcat tgaattttat tgagaatctg gattgtgtct
     4381 gaagtttgtc caatggtagt tttatttcgc tcagatcttt tgcacttttg tagatataca
     4441 tattaacacc cactccaagc gatgtcagac tatcggcttt gtagctgagt gtgacgtatt
     4501 gattggtttt tgtgttgaga gtatcctggt ataatttagc acccaggagt actgttttac
     4561 ttttccggca ggtttcttcg gtagagcatg atgaccatcc tataccaaat agtaaagcca
     4621 gaataagcca taaattaaat tttctcattt tttttattaa aaaatttctt ttacttcatc
     4681 tttcaaagcc tgtcgggcag catccagtgc agtctcttca accgattcgt acaccgtgat
     4741 tatcattgta gccaatttag aggcaggtgc ttcagccggg cattgtgcag cacaggtgtt
     4801 aattaactga attaaatttt catacgcatt ggttatactt tcccaaagtt cgttgcttac
     4861 gtaaatctgc tgggataagt tgtgctcata ttcccgcctt atttccctaa gcagtgtacc
     4921 gtgaagctca agactgttca tatctgcttc cactttgctg atcagaaaac ggtttgggtt
     4981 gatgcgttcc agcagtagca tcagtctttc ataggctttc agtcgcaccg gtgtgatggt
     5041 cgaaaggtta atttttttta attcatattg tcggcgttca ccctcatttt tgaaaagttt
     5101 atgtagcagc agataagtta caacaatcac caacactgca ggcagggtgt attttaagat
     5161 ttcggtccac atagtttcta tctttttcta ccggattgat ggattttgac atcaataggt
     5221 tgcatatata tcagacagcc tttacccatt gtttttacat ccttttcgat atgttcaaaa
     5281 aaggcttcaa gcacttcagt acggtcaatc atttccacca tcacaggcag tttttcgttt
     5341 atctcccaaa atttgctggt agaaattgtt ttactactca ggccgtaacc tgtaatcccc
     5401 cggtaagcag ttactccggc tattccgttt ttgaaagctt ccattaccag gtgttcgtag
     5461 aataaactca ttccgtattt gtcggtattg ctgacgtata ctttcaaaat gctgtattcg
     5521 ctttccattt ttatagtaga ttagaaatta aataaccaat ataaacactg agaattccga
     5581 cgagcacact tccaagaata tagacaaatg ctgtcaggaa ttgtccgttt tgtagcaaaa
     5641 taaaattttc gttagtaaag gatgaaaagg tggtaaatcc gccgcagaaa cccacaacga
     5701 gaaataattt caggtttgta ttgagtaaat cactattgac gaaaataccc agaaaaaatc
     5761 caataagtat gcttccaagg atattgacgg tgagtgttcc cattggaata ccgtgaaaat
     5821 tccaggtcag gttaagctta gcgaccagat atctggctat acttcccaga aatccgccaa
     5881 aaccaactaa tagtaattgt ctcattgaat gatcataatc aatttgtact caactattga
     5941 aagagtagaa ataaagatta ttagatttct ttttctattt tataattgtt cctttcgggt
     6001 gcgttacggg taacaagttc ggccagaaat ccggttagaa aaagcaatgt gcctaatatc
     6061 atggctgtga gggcaatgta aaaataggga ctactggtta ccagcggtga tgcaatgtgc
     6121 gaactcaggg caatgagttt tttggcgcca accactatca cagccagcag accaaggata
     6181 aacatcagac ttccgataag tccgaaaaag tgcataggtt gcttaccgaa tttggacaga
     6241 aaccacaggg tgattaaatc caggtagccg ttgacaaacc gattcattcc gaattttgaa
     6301 cttccgaatt ctcttttgcg gtgttccacc actttttcgc ctattttggt aaagccggca
     6361 ttttttgcca gatagggaat gtagcgatgc atttcaccat acacttctat atttttcaca
     6421 acatcgtggc gataagcctt caatccacag ttcatatcgt gcagtttgag gccggttacg
     6481 gtgcgggcgg tggcattgta taacttcgag ggcagatttt ttgtcagctt ggcatcgtag
     6541 cgtttctttt tccaacccga taccagatcg taatcctcct tcattatcat cgaatacaat
     6601 tcaggtatct catcaggact gtcctgcagg tcggcatcca tggtgatcac cacatcgccc
     6661 ttggcagctc tgaaaccaca atacaaagcc ggtgatttgc cataattgtg tcgaaacttg
     6721 atgccgctca cgttgggtga ttgagctttc agatcctcta tcactttcca ggaaccatcg
     6781 gtactgccgt cattgataaa aataacttcg tagctgaatt tgttttcggt catcacctta
     6841 tcaatccact cgaagagttt ggtaagtgaa ccttcttcgt tgtacaacgg tacaataaca
     6901 gaaatatcca tagtgtgtta tttgtttatt atcgttgata tataattagt tagcatctgt
     6961 gttatcaaaa atatctttat tttttttgat aaacggcgcc atgataagac ccacaaatac
     7021 acccaaaatg acattcaccc aaataaactg cagggtatag gttgccgggt tcatcatatt
     7081 ttccatgttt tggtagtaat catcgggtat attaaaatga aactgttcct gcatttgatt
     7141 catcaccgtt atgctctgtt cgagtaattg aggaagatat tcaacattca aatatttgaa
     7201 atagatgaac ttgatcaacg atgatataat agcggcaaaa agaaaaagta acaaaataaa
     7261 gaaaaatgcg tgaccaaaag tgattgttcc tccgttttct ttgtcacgat aagctaccga
     7321 aaacctgtat gtgagaacca gaatgacggc cactaccaaa tatccgagaa gtgagattat
     7381 gggattgccc gtaacagaaa gtaaaaaatt aacagaaaaa attagcccca ttatcaaccc
     7441 atatttcatg gctgcattca atacattcgt ttgcataaat tatccgcttt tattttgcca
     7501 caaaaataca aaaaaatatg gtgaatcatg caatttttta gtaacaacga ctgtataggt
     7561 gtttgcagcc gtacagataa ttgcatgata caagagattg aaatccggaa tcaggctgca
     7621 aacttttatt aatgcatgaa aatttatttt tttggcaaat tgtttttctc aaaatatctg
     7681 caccactgct tcagtccgca ttcttcacat tttggtttcc gcgctaagca aacgtatctc
     7741 ccgtggagta tgagccagtg atgtgctttt ggaattaatt cgtcaggaat atgttttaca
     7801 agttcccgct ctgtctcaag tggtgatttg gaatttgtcg ttaatcccag tcgctctgaa
     7861 acccgaaaaa catgtgtatc tacagccatt gcaggtttat tgaacactac tgaggctatc
     7921 acattggcgg tttttcggcc cacacccggc aaggtgacca gtttatctac ctcatcgggc
     7981 acttcacccc caaaatcacg tatcagtttc tgcgccattc caaccagatg tttggctttg
     8041 ttgttgggat aagacactga tctgatatat tcaaaaatta cttcgggtgt cgatgctgcg
     8101 agagcttccg gagcagggta gtcgttcagc aacttgggag taaccatatt tacccttttg
     8161 tcagtacact gtgccgagag aataacagcc accagcaggc tgtaggcatc tttgtacaga
     8221 agttcggttt ccgccaccgg acggttttcc tcaaaccagg caataatatt ttgaaatcgt
     8281 tgttttttgg tcattgtaaa aataattttg ggcaaattta acacaaagca tttatattgc
     8341 cattcatttt tcgtaatttt gcaaattagt taagattata aatgataaaa aattaacaat
     8401 ttacctcccg ataaaaattg agtatgcctc caaaaaaacc agtcaaaaaa aacatagccg
     8461 agaagcagcc ggtaaaccgc agcaaaccaa aatccacaga gccgggcttg tttacaaaaa
     8521 taattcattt catcaaagat gaaaaacttc gcttcatact ggggatatta ttagctcttg
     8581 ctgtattata tgctttattg tcatttgtat cctatttctt tacgggagct gccgacaaaa
     8641 gcgttttcga taatatcagt tttagggatt cactgcaaat acgcagttca gttcagaact
     8701 ggacatcggt gatgggagct tatatttcgg aaacattgat tgataactgg tttggagttt
     8761 cagcatttac cattctgatt tttcttttca ttgcagctct gcggctcatg aaagtgaaac
     8821 tggtctctat ctggaaagca tttttccact tatttttctg gctaatctgg acttcagtta
     8881 cactgggata cctcaccgat tttttaccct cacttcaacc ttcctttttt gcgttgggag
     8941 ggcagcatgg cactttcaca gctaccgaac tcaactcgta tgtaggtcgt cccggtgcat
     9001 tgctcataat actggcttca ctgcttatct atatgattgt ggtgtggagt gcaaccattc
     9061 cgatgattag gaatttattc catatcaagg acaaaacacc ggcatatgat gaaaatgatg
     9121 aaccaattgt agctgttgga accgaaattg ttcccgaaga atggcttgct gctgaagcat
     9181 tgaaatacaa aaccgttgtg gatgatacgg ataaaaatga agaatttacg gatgatgttg
     9241 aagatccaaa tgatacggtg ggatttgaag taaccaatcc gcacaaggaa gaaactgaaa
     9301 atccggtgga ggaatatgag gaagaggaaa attccgatcc caattataat atctctaaac
     9361 tgggaaaata tgatccaacc cttgatttat caagctacaa tcctcctaca ttagacttgc
     9421 tgaaagtgta cggatccgat aatgatatgc agatagatat gaaggaacag acagccaata
     9481 aaaaccggat tatcagcacc ttgcgaaatt ttggaattga aattgacagc ataaaagcca
     9541 ctgttgggcc taccatcacg ttgtatgaaa ttgtgccgaa atcgggtatt cgaatatcta
     9601 aaatccgaaa tctggaatcc gatatcatgc tcagccttgc agcaaccggt attcgtatca
     9661 ttgcccccat accaggtaaa ggtacaatcg gtatagaagt gcccaataat gatcctcaga
     9721 tagtttccat gaattcggtt attggttcca aacgatttac cgaagcaaaa ttcgagttgc
     9781 ccattgcttt tggaaaaacg attacaaacg aagtgttcat ggtcgatctg accaaaatgc
     9841 cacacttgct ggtagctggt gctaccggtc agggaaaatc agtgggacta aatgcgataa
     9901 tcacatcgct gctttataaa aagcatcctt cgcaattaaa atttgttctc attgacccca
     9961 aaaaggtgga atttaatatc tacgctgcca tcgaaaaaca ttttctggcc aaactacccg
    10021 atggtgaaga tgcaatcata accgatacaa gtaaagtggt tcaaacactc aattcgcttt
    10081 gcatggaaat ggacgaccgg tatgatttgt tgaaaaaggc gcatgtaagg aatataaagg
    10141 aatacaatga gaaatttgta aaccgggaat tgaatcccga aaagggacac cgcttcctgc
    10201 cttacattgt agtgatcgtc gatgaatttg gcgatttgat aatgacagcc ggcaaggaag
    10261 tggaaatgcc tattgcccga attgcacagt tggcacgcgc ggtgggtatt cacatgataa
    10321 ttgccacaca gcggccctcg gtcaacatca ttaccggggt aattaaagcc aatttcccgg
    10381 cccgggtggc attccgcgtt tcatccatga ttgactcacg taccattctg gatgctccag
    10441 gtgccaatca gcttgtgggc agaggcgata tgcttttctc gcagggaagt gaacttatac
    10501 gtgtgcaatg tgcctttgta gatacacccg aagtggaaca aatcgcacta tacattggca
    10561 atcagcgcgg atatccaaca gcattttatc tgcccgaata tgtgggacct gaaagtgaag
    10621 gtattgatac atcatcggta gatatgagca agcgcgatcc gatgtttgaa gaagcagccc
    10681 gattgatagt tgccagccag cagggctcca catccatgat tcagcgcaag ttttctattg
    10741 gctataaccg tgccggccgc attgctgatc agctggaagt agcaggcatc ataggtccaa
    10801 atgaagggag caaagcacgg caggtactcg tgcagactga atatgaactg gaaaatatac
    10861 tgaaacattt gtaacagagc cggtatttat ctcctttttt atttttctcc aaaaaacaaa
    10921 atatattatt ccagttactg acattttgtc agtaactgga aggcaactta aatttggcat
    10981 acaagttgca tcactcttgt tgtacattta attatcatta aaatgaaaac aaaaaagata
    11041 gtttacacct tgttaatggc actttttatt ttccctgcca cctatgcgca aagcagtgcc
    11101 aatgccgtaa aggtggtaaa tgacatgatg aatttgctca aaacaaatgc tattagtacc
    11161 aattttgtaa tgacggtaaa agaacctact tccacacaag ctcaaagtat aaaaggcaac
    11221 tttacaatga aagaaaataa gtttatcctc aatactgatg aaatgaatgt gtattttgat
    11281 ggaaaaactc aatcggccta tatgacatcg gtgaatgaag tatcaattac taatccaacc
    11341 gaaaaggaac tggccgaaac caatccactg gctgtactgt cggcttataa aaacaaatcg
    11401 atcattaaat tttccaaaaa aagtcagtca aaaacagttc atgccataga actgacacca
    11461 aaagaccaaa attcaacgat aaaaaaaata cttgtaaacg tgaacaaaag caataattat
    11521 ccgttgtttg ttcagttgac cgatagaaaa ggtgcgatca gcaccctgac tttatcacaa
    11581 ttcaaaaccg gactgaaaat attggattct tctttcgttt ttaatccaaa aaaatacaag
    11641 gatattgaaa tcaatgattt gaggtaatga tattatattt ttaatttgaa taaaacaatg
    11701 cgttaattta agccgaatga tgaattaacg ctttgtattt taaataatta ctaacaaaaa
    11761 taataataaa attaaacata aaaaaatatg aacgaaaaag taagatgcct gatcataggc
    11821 tcaggccctg ccggatatac tgcagccatt tatgcttcca gagccaattt ggctccggtt
    11881 ttgtatgaag gaatgcaacc cggcggacaa ttaacaacaa cgactgaagt agaaaatttt
    11941 cccggatatc ccgaaggaat taccggaccc gaattgatgg aagatctgaa aaaacaagct
    12001 gtccgtttcg gcgccgatat ccgttttggt atggtttcgg caaccgattt atccgcaaca
    12061 ccgtacaaag tgactattga tggtgaaaaa gtaatagaag cgcaaacgat tatcatttcc
    12121 accggtgcta cggccaaata ccttggtttg cccgatgaaa cgaaatatgc aggttcggga
    12181 gtttcggcct gcgcaacctg cgacggattt ttctatcgca aacgcaaagt cgccgtagta
    12241 ggtggtggtg acactgccgc cgaagaggct gcctatttag ccaatatagc tgataaggtg
    12301 tatttaattg tacgtaaaag ctacctgaga gcttctaaaa tcatgcagga aagggtaatg
    12361 acaaatccta aaattgaaat tctctttgaa catgaaactc ttggtctgac cggcgataag
    12421 gtagtggagg gagttgacct ggttaaacgc cgtggtcaac ccgatgaaga aaaagtccat
    12481 attgaagttg acggattttt cctggctatc ggccataccc caaattcagg cattttcaaa
    12541 ccctggatag aaaccgacga agtaggatat attaaaacca tccccggaac tcccaaaact
    12601 aacattgaag gaatttttgc tgccggtgat gtggcagacc caacttacaa gcaggcaatt
    12661 actgctgccg gtacaggttg tcaggctgct attgaagcag aacggtattt atccgaaaaa
    12721 ggattatagc agaagtatca ataaacaaaa aaaagcagcg aaaaccgctg ctttttttgt
    12781 ttcacttact tatttcattt aatttcttcg agcatccgaa gcgctacttc caatatactt
    12841 gtatcatcca gttgcacaat tcctccgtca ggtcccggtt caatgtgaag tcctacgctt
    12901 ttcccttctt tataatcact accgccgaaa taatttttta ctgtttgttt ttccagacca
    12961 agttcttcgg caatcttggc catactcccg cttggcagtg catctttaat tctgcgcaat
    13021 tcgttaaaag ttactgtctt tgtcatagca aatgaagttt tatagttaat actttaatgt
    13081 taatttttgg tgcaataaac gtagtacttt aattttgttt ttccaaacat tttggcacaa
    13141 aaaaaactaa attgtttcaa atatattacg ttgaaattac agatatatga tattcaacag
    13201 attatctttt ttctgaatat tggttgtaaa attcaacgat tgtttcaatt cctttttcga
    13261 ataaatcaag tggaaaattt tcgttgggag aatgtatagc atccgactcc aatccgaatc
    13321 ccatcagtat cggtttaact tttaatattt tttcaaaaac gggtaccaca ccaatgcttc
    13381 cgccacgccg aaccggtagc ggtcgtacgc caaaaacttt tgtgtaggca ttttcggcag
    13441 ctttgtattc ctcagattct atggggcaga cataactttc ggctccatga agctgtgtaa
    13501 cttttacgtc tacatatttt ggggcaattt tcagaatatg tttttcgacc agtcgggcaa
    13561 ttttttcata cttctgatgc ggtacaagcc gtgccgaaag tttggcatac gcttttgacg
    13621 ggagcaccgt tttacttcct tcgccggtat agccgcccca tattccacaa atatccagcg
    13681 ccggacgaat tccggtacgc tcaatggtgc tgtacccttt ttctccgaac acctgtttta
    13741 ttcccagatt ggatttgtat tcttccaggt cgaagggtat ttgggcgatg agttcccgtt
    13801 cacttttcgt aactccggcg acgtcgtcat aaaattgtgg tacggtaatt cgaccgtctt
    13861 tatctttgag ctgtgcaagt atttttgtta gttcaagaat aggattggct actgcaccgc
    13921 caaaaattcc cgaatgcaaa tcgcgattgg cagctgtaac ctctatttcc cagtaggcca
    13981 gtccgcgcaa tccggtagtg atacttggtg tatgggccga tagcatgctg gtatcagata
    14041 ccaatatcgt atcgcatttc aactttttca gattattttt acaaaattct tccaggctgg
    14101 gcgatccgat ttcttcttca ccttccagta tgaatttcac attgcatttc agctgatttg
    14161 tcctgactaa atactcaaaa gcttttgcat gcatgaatga ttgacctttg tcatcgtcgg
    14221 caccccgtgc gaaaattttt ccatcacgta tttcgggttc gaagggtggc gtatcccaca
    14281 aatccaacgg ttcagccggc atcacgtcgt aatgtccgta aaccatcacg gtgggcaatt
    14341 tcggatcgat taatttcgaa gcaaatacaa ccggattgcc gctggtaggc agaatttcgg
    14401 ctatatcgac gccggcttct tcaagcaact gcttccatcg ttcggcacat cggtgtatgt
    14461 cgggctgccg gctggcatgg gagctaatgc tcggtatgcg gattaagctg aaaagttcgt
    14521 tcaaaaagcg ggtctcatgg tctctgatat acgatttaat ttccatattg atgtgtttaa
    14581 ttattttgaa tttgattgaa acattggttt tttttattgc gtaaactcta catgataatg
    14641 atgcaggaat atgtaaaggt aaaataaatt gaattatttt caactcaaaa ctgaaaataa
    14701 ctcaattttt cttttttacc tgatattaaa aacacaaaat tatttatgaa tctaatacga
    14761 tatttgtgct atactgataa tttaactaac tttgcaaagt ttgttttatc cgttttgtac
    14821 taattacaat ccataaaatg cttactaaaa caggtatgaa aaaaatcaac ataatattaa
    14881 tagtatcaac cttactgata tttaccggct gtaccactca aaaaaaactg gcatatttta
    14941 ataccgttac ggcttcctcg gccgaaagta tcaatgcgca gctcaaaaat gaaagagcgg
    15001 atgcaagaat attaactgat gatcggttgt caatcactgt ttcggcactt gaccccaatg
    15061 ctgtggccat ttacaacctg ccttttgtct catttgcatc gcccggctcc gatcagattt
    15121 atgcatcacc cgttttacaa tcctatcttg ttaattcaca gggaaatatc aattttccgg
    15181 ttttgggcga aatcaaactg gaaggactga cactaaccga agccggcaat cttattaaaa
    15241 ataagttagc tgaacatgtg gcagacccaa tcgtaaatat acaatttgtc aactttagaa
    15301 ttacggtttt gggtgaagta cttcgtcccg gtcaattccc ggttaccaac gaacgggtga
    15361 ccatattgga tgctctcggt cttgccggcg atatgacagc ctatggtcgc cgcgataatg
    15421 tattactcac ccgcaacaac aatggtaagc tcgaatttgc acgtattaat ctcaattcag
    15481 acgaggtttt caaatcacct tatttttatt tacaacaaaa cgatgtgatt tatgtagaac
    15541 caaataccgt gaaatcaata tcttcacagg atatccctct ttacttgtcc tctttatcca
    15601 ctttggctac cctggttaca cttatttact ctatttcaaa gaaataattt accggattaa
    15661 ttttgctgta gttctttttt tagaaaataa atttttatgg agaaaaataa taataacatt
    15721 aacaatagca ttaataatac agaagtaatt aatcttcgtg aactactcac cagatacgtt
    15781 cagaaatggt attggtttgt gctggcagta atcattgctt ttatagtagc tacgatttat
    15841 ctgagaaaaa cggataataa atatcaggta cagacggtaa tattgctccg caacgaagat
    15901 aattcaagcc agatttctca gcttgccatg atggagagct tcggattaat gggttcttcg
    15961 cgtgtggttg atgatgaaat tcaggtgatt aattcaaata aaataataaa tcaggtaatt
    16021 gatacacttg gaattgaggt tgagtatttc gaaaaaaccg gtcttaaata cgttgaaaaa
    16081 tatcatacac ttccctttaa acttaacctt gtaaatcatt ttgccgattc actgaaagca
    16141 cccatccgaa taaccctgaa aacaggtaca ggttctgtaa aagtaagtgt gaaatatgga
    16201 cgtgattgga aaaatactta tactttagat aatatccagc aacccttcag cactcctgca
    16261 ggcgtattga gcttaaaatc actgtcatcg gtaaaacccg ggatgaggta caaaatcaat
    16321 gtctatccca aaaaagacct gctggcgcaa tatcgcggaa aactgaatgt atcggttgtc
    16381 aacaagcaat ccaatgccat tcgcatttca attgttgaat tcaatatcaa aaaagcggaa
    16441 gatattttga ataaaatcgt tgaattgtac aatatggacg caatcatcga taaaaatatc
    16501 gtagctgcca acaccggcaa tttcatcgaa gaccgattgc gactgatcac aaaagagctt
    16561 tttgatgtgg agtctgaggt tgaatcttac aaaaaagcca atagcctcac cgatatagcc
    16621 tctgaagccg gaatttatct ggcatcagcc agcgaatacg aaaaaaagat tgccgagttg
    16681 gaaacgcagc taaacctgat acaatatatc gaaagctata tcagggatgc caaaaatcaa
    16741 tacagcttaa ttcctgccaa cattggcgtg gaagataaat cgctgatgga attaatcctc
    16801 aattacaacg ctattgtact ggaaaggatg aaattgtcgc agtcggccaa cgaaaaaaat
    16861 cccgtattga ttcaaataga gcaacagata aaagccctga gaggcaatgt tctcgaaagt
    16921 attggaagtg taaaatctgg actgaaaatt gccaaaaatg acgttacccg aaaggatgct
    16981 cagttcaatt cgagaataaa agctgtacct acccaggaaa gacaattcct ggaaataaaa
    17041 cgccagcagg aaatcaaaca aaacctgtat ctttttctgg ctcaaaaaag agaagaaaat
    17101 gcattgagcc tggcaagtac cgctcccgct gccagaacta tcgatcgtgc atatccttca
    17161 ctcacaccgg tttcacccaa aagaatgatt atctatctgg tggcgctcat tttaggatta
    17221 ctattcccat tcttatatat ctacctgcgt gatttgatta ataataaaat tgaggatgcc
    17281 aaagaattcc aaaaaacggt gaatccgcct ttcctgggca gtatctgcat aagtcgagag
    17341 tccgatagag tagtggtgca agccggaaaa acaacgccta ttgttgaaat gtttcgtttg
    17401 gtacgtacca accttcaatt tatcatcgga agcaagaaaa atcctgcaat tctgattact
    17461 tcaagcatca gtggcgaagg gaaatcattt atttctatca atacagccat gtcgctggcg
    17521 ctgctcaata aaaaagtagt attaatcggt atggatattc gcaatcctgt gttaagggat
    17581 tatatgcatt tatcaaacgt aaacagcaat ggtgtgacgc tttacctgtc ggatacagat
    17641 tgccagattg gcgacatcat catgccttca ggttataaca acaatttgga tgtaatccct
    17701 gccggaccgg ttccgcctaa tccctctgaa ttgctgctta gtccccgact tgatgaactt
    17761 gtactggaac tgaaaaaaat atatgactac attatcattg actctgcacc aataggcatc
    17821 gtatctgaca catatctgct gaacagggta atcgataact gtatctacgt agctcgtcag
    17881 gatcatacgc cccgcgaagc aagcaatctg atcaatgaaa tttatgcaga aaaacgattg
    17941 aatggtatgg cacttattct gaatggtact tcggccacat ccagttatgg atacggatat
    18001 ggatacgaac gtaataaaaa caagtttaag aatatgccaa aactcacttt cggcgataaa
    18061 ctgaataatg ctatattgaa attattcaaa cgaaattaac tcataaattc aaaaagaata
    18121 aaaaaacctg atttagtcag gttttttttg tggggaaaca ggaaacttaa ttattatcat
    18181 agtttccaat cggttggttg caaacaactg tatatcaaca atataaaatg tcataatatt
    18241 tatttaacaa aaatatattt gaaatataat cattgttacc tgttttttat atagttttgc
    18301 acaatcaaga acaaacatgg atacagtagc cgaaaatata attcacactt ctcatcaaaa
    18361 attcaaaaat ttcgggatta gaagtatatc agtagatgat atctgcaacg aattacatat
    18421 atcaaaaaag acattttata gccgttttcc ccaaaaggaa tgtcttatag aagctgtgtt
    18481 ggattatgag tctgaagtca gtactgacaa ttttgaaaaa acgtataaga acaagaatgc
    18541 gattgatgct ctgattatta taatcaagga cttaaaaaac agcattgata aagccccgct
    18601 aacatttcat tacgatctgg aaaagtatta tccggctact ttcacaaaat acaaggagcg
    18661 acaatcgacc cggatacgca attgttttga aataaatcta cgaaaaggaa tagaagaagg
    18721 ttattaccgg gaggatatag atgtggagct tgtatcactg tttcactcca ttcaaatccg
    18781 ggaaactttt gagcaaatgc aaaaagcttc gcctaaaatt acaaaaaaac agttggtgga
    18841 tttttttatt gatttaatag taagacttat caccaacgaa aatggatata agtacatgaa
    18901 cgaaaattat tacaaactta aagattaaac agataaaata tttagataat gaaaaaaacc
    18961 ggtttattta tattaggact aatgttggca ggcaatatag ctgctcagga taccattgtt
    19021 ctgagtttgc cccaggcact tgacattgca ctgagcgaga atcctacaat tcggatagcc
    19081 aacaaagaaa taaaaagggt ggaatataat aaaaaagaga aatacggagc gttattgccc
    19141 aatatttcac ttggattatc gtatgcacga actctaaaga aacaaaaaat gtttttttcc
    19201 attccgggta tgcctgcaaa tcctgatggg atagaagtgg gtcaggacaa caccttcaat
    19261 ggctctacac agggtctcgt ggcttccctg cccttgtatg caccggcact ttggcaatcc
    19321 attaacatgt cggaactcga tatggagctg gcactggaaa gcgcacgatc atccaagatt
    19381 tcgcttgtca accaggtatc gaaagcgtat tataccattc tgatggctca ggactcgtac
    19441 aaagttctca tgcgcacctt cactaataca accgaaaaca accggattgt tcagaataaa
    19501 ttcaaacagg gtgtagtatc agaattcgaa tcaattcgcg ccgaagttca actgcgcaat
    19561 gtggccgcaa atctttcggc tgccgaaaat gcggtagaac tgaccaaatt acaacttaaa
    19621 atgctgatgg gaatggatat cgatcaacaa attaaagtgg aaggcaattt gtcagattat
    19681 gagaataaaa tgtatgccga tgtactgaaa attgatacta ccacactggc ttcaaattct
    19741 gatctgaagc aatttgacat taaaacccgc caactcaacc agtcatcgaa actccagcgc
    19801 gcgtcatgga tgcctacgct tgcagccagt tttaattaca attacatgtc atatgccaac
    19861 gatgatatta ttttcaccgg agatcaccgc tggtttccca cttccaatat cgggttagta
    19921 ctttccattc cactttttca gggagggcag cgctacttca aggacaaaca aatgcaaatt
    19981 cagattgatg aactgaagga tcagaaactc aacctgaaac ggggactgga actacaggca
    20041 atgacttata tcaataatat gcagaaagct atcaaactca tagagtccaa taaagtggcg
    20101 ctttcgcagg ccgaaaaagc aatggcaata tcccaaaaac gatatgaagt gggagccgga
    20161 acttaccttg atgtagctaa tgccgaactg gcctaccaac aatccggctt atcatacaat
    20221 cagtcggttt ttgattacct gtcggcaaaa gcagatttgg aaaaactgct cggaaatgaa
    20281 aaaactaatt aaacgtaaaa atataaatat tcaaaaatat gaaaaacttt aaacttatta
    20341 caatcagttt acttgccatt gctttgagca gtacgtcatg tacaaaatct gataaaacca
    20401 agacccaatc ttccgatgcg aaaattccgg ttaaaatagc acaagtttcc gaaaaaaatg
    20461 taccgcaaat tgttgaatat ccggcaactg ttcaacctgt gtttaaaaat aatatagcac
    20521 catcggcacc cggacgtatc cgccgaataa tggtagatgt gggaagccgg gtaggagcag
    20581 gccagcgaat agtgcagatg gatgcagcca atctggcaaa ctatcaaact cagattgata
    20641 atcttcgccg aaattacaac cgtgtttctg aattattgtc ggtaggtgga gcttcgcaac
    20701 aggatgtgga taatgtgaag gttcaactgg aacaagctga gaacacactg aaaaatctaa
    20761 acgaaaacac atatctcaca agtcctataa gcggcattgt cactgcacgc aattacgata
    20821 acggcgatat gtattcgggt caactgccgg tgcttacggt gatgcaaatc aatcctgtaa
    20881 agattttgat caatgtatcc gaatcctatt attccaaaat taaagtgggt atgggtgttg
    20941 atatgagttt tgatgtattc cccaacgaaa ggttcaacgg aaatgtaagc cttatttatc
    21001 ccaccatcga tgaacgaacc cgcactttta ctgtcgaaat caaattacct aacaacaata
    21061 gcaaagtccg acccggcatg tttggaagag tgattgtaaa ttacggatac gctaatcgtg
    21121 tggtagtacc cgatttatcg gtaataaaac aggctggttc gggagcccgg tatgtatttg
    21181 tgtacaatga cggaaaagta acctacaaac aaattgagat ggggcagcgc attggcaatg
    21241 aatatgaaat actgtcaggt ttgagtaccg gtgatcaggt ggtggtggct ggtcagtcca
    21301 aattggtcga tggatcgatg gtcgaaatta ttaaataaac actttttgat aatcaagact
    21361 ttaaggtagt ctgtttgctt aatcaagcta agacttttct aaatgttgaa aaataaaatt
    21421 ttaaataaat taatatgagt ttatacgaaa gttcggttaa aaaacctgta atgacctcga
    21481 tcatttttat tgcggtggtt attctgggta ttttctccta caataaattg ccgatagatt
    21541 tcttgcctaa aatagaaacc aataccattt tggttatgac aacctatagc ggtgcaagtg
    21601 cttcggatgt tgaaacaaat gttacccgtc cgctcgaaag ttcattaaat acggtctcta
    21661 atttgaagaa tattacttct gtgtcaaagg aaaatacttc gattattacc atggagtttg
    21721 attacgggct ggatttgaat gtggtcacca atgatgtgcg taataaactg gatattgtaa
    21781 aatcaataat gccagcgggt gctggaagtc caataatatt caaactgagc accgacatga
    21841 taccggtaat ggtaatcaca gcgacagctg atcaaagtct gccggcactg tacaaaattc
    21901 tggacgataa ggtgggtaat ccgcttgccc gtatcaatgg tgtgggatcg gtgtccattt
    21961 cgggagctcc tcagcggcaa attcaggtat ttgtggatcc tgtaaaaatg gaagcctatc
    22021 atctgacggt ggaagcactg gcacagtata tccggatgga aaatgtgaat acgcccgccg
    22081 gatcgatgga tgtaggcagc gatacctacg cattgcgtgt acagggagaa ttcaaggatc
    22141 cctctgaact gaataatatt gtagtgggaa attataacgg aaaaacagtt ctcttgtcgg
    22201 atgtagctgt cattagcgac aaacacgaag aacgcatgca ggaaagcttt accaacggaa
    22261 cacagggtgc ttctattgtt gtacaaaaac agtccggagc caacaccgtg cagatagcca
    22321 ctgaagtgaa aaaaaatctt cccgacattc agaaaactct gccatcagat attaaactga
    22381 atgtgattat ggatacgtca gaaaatattc tgcgtactgt aagcagtttg gaagaaaccg
    22441 ttttatttgc actcttattt gtaatgctcg tagtgttgtt tttccttggg cgctggcgtg
    22501 ctacggttat cattattctt actattccca tatctttgat ttcagctttt atctatctgt
    22561 accttaccgg tggcacgctg aatatcattt cgctcagttc gttgtcgctg gctataggac
    22621 ttgtggtgga tgatgcaata gtggtactcg aaaatatcac cacacacatt gaacggggcg
    22681 gccgacccaa accggcagcc atcaacggta cgaatgaagt cgggctatca atcgtagctt
    22741 ctacgctcac cattattgcc gtgttccttc cattgacgat ggtaagcggt atggccggag
    22801 taatgtttgc gcagctgggc tggatggtaa cgattattat taccatttcc atgatatgtg
    22861 ccctcacact cactcccatg ctttcgtcac agttattgag actcaacccc aatagaagca
    22921 agggattcaa tatgatatat ggaccgatag aacgactgtt ggacaacctt gacaagaaat
    22981 atgctcagat agttaactgg gcggttcgtc acaaaaagtc agtcatgctt tttgccactt
    23041 tatttttctt tatcagtgtc acgccaatga tagtggggtg ggtcggtacc gaaaactttc
    23101 cggctcagga tagtggtatt atctcaacaa caatagaatt gcccgtaggt acacgtatgg
    23161 agctcacaag agatgtttgt cagaaactga atgatgaatg gatgaaaaaa tatccggaaa
    23221 tcaggatgct taattacaac gtaggacagg caagcagtaa aaatgttttt ggctccttgt
    23281 ttggaaaaaa tggaagtaat atcggttctt tttcaattaa gctggttgaa ctcgaaaa
//
LOCUS       DAOPZK010000015        23085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2233_length_23085_cov_20.795050, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000015 DAOPZK010000000
VERSION     DAOPZK010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 23085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23085
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_23085_cov_20.795050"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="tsaB"
                     /locus_tag="PKH68_01810"
     CDS             complement(<1..681)
                     /gene="tsaB"
                     /locus_tag="PKH68_01810"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNX11699.1"
                     /translation="MTILHIETSTHVCSVALSHDGDCIFEISNSEGSNHARLLSLFVQ
                     QALDKLKELNLPLDAVAVSGGPGSYTGLRIGVSTAKGLCYGFNIPLIAVSTLEVLCVE
                     ALKKLENKENVLLCPMIDARRMEVYTAFYTPKLEVKQEISAEIITEGSFDNLLAENPV
                     YFFGNGAAKCAATLTHPNAHFLIDIHPLAKNMIALAERKFEAGDFADVAYYEPFYLKE
                     FQATTPKSL"
     gene            complement(759..1433)
                     /locus_tag="PKH68_01815"
     CDS             complement(759..1433)
                     /locus_tag="PKH68_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX11700.1"
                     /translation="MLQNVNKQIFNILLPGLLLLIPFPALRAQTVLLDPPEIYIGATG
                     GMTGSMVMFNPTVNQSMPLLAYNGGLSIRYVTESHCGLQIEANYSQRGWEEAGGNYSR
                     RADYVEFPLLTHLYWGKTNRFIFNLGPKLSYLLKETDLINTVAEPQEQQKTPIYNPFD
                     YGIAAGFGYNLHTRKAGVFQLETRAYYGLSNIFADAKTDYFKASNFLNVSVSLGYYFQ
                     LTGKKD"
     gene            complement(1414..3237)
                     /locus_tag="PKH68_01820"
     CDS             complement(1414..3237)
                     /locus_tag="PKH68_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNX11701.1"
                     /translation="MKLKISVTLLIILTLCSAIIYAQKPVYSTKVIDGTEYYIYQVEA
                     GEGLYTISKKFGVSQADINNANPQIHDGIKAGQEILIPKTGKQIQPETNNSNDKDGFI
                     LHTVEKKQTLFAISRKYDVSQEDIREANPQIANGLKTGDVLRIPVKRRNQDTSVKPKV
                     EKEEKKADKLPKPVESTTAKSAPVPATLHIGTNETYFIHRVEIKETLYSISKIYNVAV
                     DDIVRLNPESATTLKTGTELKIPYNTRTTVNGNGEVKLKPVTEKTSYKIAYLLPFMLD
                     GEKQDPTVEKFLEFYMGSLLAINNAKRGNFKIDVYTYDTEKSEVKINEVLNKPEMQTM
                     DLIIGPAYTAQIPVLIDFSNRRKIPTVIPFSSSIKNIETNPYVFQFNPDNDIHNDYAA
                     AVLKNKFSDANIVFAQTGNTKWSEDGTDFFRFLQKKLDKLNVSYTKIAADILSVKTIE
                     TNLSTAKRNVIVFDTEDLSSVQTYLNKLYELSSSNDVSVLGQYSWRGKNGKKPKMYYI
                     APFDHNDASPEKIQYENDYRLFYKQFRSEKNPRFDMLGYDITNGFVKQMTNNGFRFNE
                     TTSSVKFDQGIQSDYNFKRTRDGGGFINQQLYLIEDAPKRK"
     gene            3479..>4731
                     /locus_tag="PKH68_01825"
     CDS             3479..>4731
                     /locus_tag="PKH68_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX11702.1"
                     /translation="MKRTFLFFTLLLFTVISFSQIQVRGNYTSYDEASGLDHIYIFSV
                     IDNSSEIHYTSTGNTALVKWFTFLNGVKTELTSVSIVSPTETYIDPKNNTGYIINVNG
                     TDIARFWVFDYSQYIPVFTSLNGTDGEFPCENVALQLVGNVPEFKYQNTGGGVFTIDR
                     YFNIEYNTLEWDTEKKEWKVKIQTEEIKQPGSVIPLPTPSTNTTFTLKGDQFASQLNM
                     PEASVTSGEYVTKAVKCKITTVTSVREAKNENDRPEKETDIEGSAPLDIQFYSNPTPN
                     VRSYFWTIYRNGEKLMARSEKDHLYTFSKAGNYKIELSATNGICTDSSSVNVVVMESS
                     IIAPSVFTPNNDEYNEEFRVAYRSIIEFACTIYNRWGRVVYKWTDPAKGWNGTIGGKP
                     AAEGTYFYIINARGSDDKVYKLKGHL"
     assembly_gap    4732..4831
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4832..5902)
                     /gene="gcvT"
                     /locus_tag="PKH68_01830"
     CDS             complement(4832..5902)
                     /gene="gcvT"
                     /locus_tag="PKH68_01830"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNX11703.1"
                     /translation="MKSTPFTDIHIALGAKMHEFAGYNMPIEYSGINDEHLTVRTGVG
                     VFDVSHMGEIWVKGPKALDLIQKLCSNDASKLNVGQAQYSCFPNGKGGIVDDILVYYF
                     EPEKYMLVVNASNVEKDWNWLNDNNTVGAVLENASDHTAQLAVQGPKAAELLQKLTPV
                     NLSEVPYYHFTVGEFAGVANVILSNTGYTGAGGFELYFYPEHGVQIWNALFEAGKEYD
                     IKPIGLGARDTLRLEKGFCLYGNDIDDTTSPIEAGLGWITKFAEGKDFTDRALLEKQK
                     AEGTARKLVRFELTERGIPRHGYDIVNGEGAIIGHVTSGTMLPDSKKGIGMGYVNAPY
                     FKNGEPIFIQIRNKNLEAVIVK"
     gene            complement(6012..7238)
                     /gene="pepT"
                     /locus_tag="PKH68_01835"
     CDS             complement(6012..7238)
                     /gene="pepT"
                     /locus_tag="PKH68_01835"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNX11704.1"
                     /translation="MNITDRFLKYVSFTTTSDENTNMTPSTPGQMIFAKYLADELKSI
                     GMKDITLDKNGYIMATLPANTDKEVPVIGFISHMDTSPDSSGKNVKPRIVENYDGGDI
                     LLNEEKLIVFETEKYPEILQYKGQDIIVTDGTTLLGADDKAGLAEIVTAMEYLIQHPE
                     IKHGKIRVGFTPDEEIGQGADHFDVKKFDAEWAYTMDGGEIGELEFENFNAASAKVTI
                     TGLNVHPGYARHKMKNSMRIATQFVSMLPRHETPEHTEGYEGFYHLTNIEGTVEKTLL
                     TYIIRDHDRDRFERRKKEIQHLANKINAEYGENTAVVELRDQYYNMREKVEPVMHVVD
                     LAFEAMNATGVKPNVKPIRGGTDGARLSFEGLPCPNIFAGGHNFHGRFEYLPIPSMEK
                     AMMVIVKIAELLEQKS"
     gene            complement(7362..8279)
                     /locus_tag="PKH68_01840"
     CDS             complement(7362..8279)
                     /locus_tag="PKH68_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX11705.1"
                     /translation="MAIPTSRTSERGNISIDIEKCNGCGLCVSVCKDFDITLQNGKAV
                     LSNTDVFGCIACGHCMAICPEGAITISGRTASPADLFDLPDKERMAGYDQVLALLQHR
                     RSIREFKDQPIDDVITEKILKAARTAPIGLPPSDVNVLVINSKDKNTEFVNDFSHLLE
                     SMKWMASKPALALMRLFMSKSNAEMFASFIAPMIDLFVTSTAEGKNWLTYDAPLVMYF
                     YGTPYSDPADPLVAATYAMIAAESLGLGTCMIGSIHPFIQYGKKARQFREKYQIKYKS
                     REGLVVIFGHSSVHYRQGITRTFASEIML"
     gene            complement(8329..9456)
                     /locus_tag="PKH68_01845"
     CDS             complement(8329..9456)
                     /locus_tag="PKH68_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNX11706.1"
                     /translation="MLSNIFNKEVEQLFTTPIVQQTAFWSIVKKKLGVSTLAINFKSN
                     KSFLYNNSESKDDNIVSDLLVIVRAINQNHSIAYVPYGPELEPDDEFQGLFLEELSEC
                     IRSFLPKNCIMIRYDLCWESYWAKEKDYFDDFGHWKGEPEVKTQEMRFNFNTINWNFK
                     KSLSNILPSNTIYVDLLKKNEEILADMKPKTRYNIGLAYKKGVVVKSSDIENIDIWYK
                     LYKETAKRNNILVNDIKYFTAVLSARTNDVRSPAEVKLLIAEYNNQPLAAMFLVITGN
                     RGTYLYGASSSENRNLMATYALQWEAIKISKASGCSEYDMFGVSPNPDPNHPLYGLYR
                     FKTGFGGEIYHSLGCWDYPLDVEKHRYFTALELTEQGYHLN"
     gene            9692..11836
                     /locus_tag="PKH68_01850"
     CDS             9692..11836
                     /locus_tag="PKH68_01850"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNX11707.1"
                     /translation="MRSGYLTVLILFLSCKFIFANNPLPLDSVLHQAMNAAESYSGLV
                     SDYESEVYMRAYVKTNKKNFLYKYTYLIPGFVLHDRKNDEGLIESISKLRMASSNIYF
                     QDVNYINGTMTRRSDIAMLPFRFMSLNIYKESAPDESYLLPLRKTSAGYYQYKLISVE
                     TADHKPEYTIEYTPRYKNSKLISGTFVLEDSTWRVKHFTGKGSDLFFNFSFEITMGQD
                     LINSYLPVKFAIRQAYTYFGNKVVNRYNAILTYKNVKLKEQIETRKNLNLGNRYKVRM
                     DSVPANVDSTFWNKIRPLPLSENEQLLLSKYAEKRKKTQTEQQKEDTTGIKSATALKF
                     AKNMVLDSKYRYKSTSIRYSGVFNPSMISYSSEDGLTYRQKLFLGIELARHQRINIDA
                     FAGYVFNKKEFYGDLSAVWNYDPAKFGNLSFSIGKGNQSYSSLFLNMVKDSLTSQGLN
                     IEDLGVNYFKDFYVRVFNSVEITNGLQLGAGFDYHIREPNKSQMSASGPNHEQMDGIF
                     DTRYYFVPVISLSWTPEQYYTMDKNQKVYVRSEYPTFKIQYAHSLKNVFGSTSQYSRV
                     EFDINQNIRFDLMKSLQYHVGTGFFSRQKTEYFTDFVFFRRSYFPETWGEGIGGVFNV
                     LPRNLYNASSSYIQAHLMYETPRFLLTRIQALSGGVVKERLYYSQLFTPTIPSYSELG
                     YGVGNRFLNAALFVSFFKLSYQNIGFKSVFLL"
     gene            11861..13720
                     /locus_tag="PKH68_01855"
     CDS             11861..13720
                     /locus_tag="PKH68_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNX11708.1"
                     /translation="MPIFVPDIQNVHNMQQITQLTDFLPTTKKELELRGWEEVDIILF
                     SGDAYVDHPSFGSAVIGRMLEAEGLRVALIPQPNWQDDLRDFRKLGRPRLFFAVSAGN
                     MDSMVNHYTANKRLRSDDAYSPDGRAHMRPDYATITYCNILKKLYPDVPIVIGGIEAS
                     LRRVTHYDYWSDKLMPSILVDSKADFLVYGMGEKPLRELIRQLWEGKKIGEITDIPQT
                     SYIANEIPEVENLQTIELVSHEECLKDKKKYASNFRYVEEESNRMQGARLVQKVGRKH
                     IIINPMYPPWSPEEVDATYRLPFTRLPHPKYKGKTIPAYEMIKFSVNLHRGCFGGCAF
                     CTISAHQGKFIVSRSKQSILDEVRQITKQPDFKGNLSDLGGPSANMYAMQGRDLSICV
                     KCKRPSCIHPAVCPNLNINHSALTDIYRSVDKIPGVRHSYIGSGIRYDMLTAQSRDAE
                     TSRSQQEYLRELITKHVSGRLKVAPEHTSERVLKLMRKQSFQDFRKFKELYDRINSEA
                     GLNQQLIPYFISSHPACHKEDMADLAAQTKDLNFHLEQVQDFTPTPMTLATEIYYSGY
                     NPYTLEPVFTAKTKEEKLEQRKFFFWYKKEYRQQIMRDLQKMNRPDLIRKLFG"
     gene            complement(13773..14864)
                     /locus_tag="PKH68_01860"
     CDS             complement(13773..14864)
                     /locus_tag="PKH68_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNX11709.1"
                     /translation="MRLNSISILNYKNICQADLEFPSKINCFVGNNGMGKTNLLDAIY
                     YLSFCKSYSNSIDSQNIEHNADFFMLQGKYTLGDTEEEIYCGMKRRQKKHFKRNKKEY
                     DRLADHIGLLPLVLVSPGDDELIADGSDERRKFMDSVISQYDKTYLNSLLNYNNALKQ
                     RNALLKEEALTDESLYEIWEEMMVKYGNYIYTRRKDFVEEFIPIFQEIYRFISNDNEQ
                     VHLNYKSQLTDADLKQKLQANRERDRLIGFSMNGIHKDELEMMLGDYPMKRVGSQGQN
                     KTYLISLKLAQFNFLKRTHGIAPILLLDDIFDKLDALRVKQIVELVAGDNFGQIFITD
                     TNRKHLDDILSQIGQSSQIFEIVNGEIIQ"
     gene            15068..17191
                     /locus_tag="PKH68_01865"
     CDS             15068..17191
                     /locus_tag="PKH68_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNX11710.1"
                     /translation="MVSEELIKQLTQSTSYSFHQVQNVVRLIDEGATIPFMSRYRKEA
                     TGGMDEVQLADLREQYDKLCELIKRKATVLKSIEEQEKLTPELRQRIEECWNANELED
                     IYLPYKPKRRTRAEIAREKGLEPLAKIIMKQQTVDIQNVVNRFITSDVMNASDALQGA
                     RDIIAEWVNESEAARNAIRSIFSHEAMISSKMVKGKEEEAQKYRDYFDCSEKLSRCSS
                     HRLLAMRRGESEGFLRVNITADDDKCIARLLRIFQKSDNECGLQVAEAVEDGYKRLLK
                     PAIETEFAADSKSKADVEAIRVFAENLRQLLLAPPLGQKRVMGVDPGFRSGCKIVCLD
                     AQGNLCHNENIYPHTPQNEHSQAKRKLQKMVEAYDIQAIAIGNGTASRETERFIQSIS
                     FDRKVQLFVVSENGASVYSASKVARDEFPEYDVTVRGAVSIGRRLMDPLAELVKIDPK
                     SIGVGQYQHDVDQTKLKNSLDRTVEFAVNRVGVNLNTASKHLLTYVSGLGPQIAQNIV
                     NFRAENGAFRNRKELMKVPKMGAKTFEQCAGFLRIPDGDNALDNSAVHPESYPVVNQI
                     ATDLKLKVNELIGNNKLKSTLDLSKYVTEKVGIPTLNDILDELEKPGRDPRSAIRIFE
                     FDPNVKDINDLRTGMELPGIITNVTNFGAFCDIGIKENGLIHISNLSDSFVSNPADIV
                     SVHQHVKVSVLEVDLARKRIQLKLI"
     gene            17223..17945
                     /locus_tag="PKH68_01870"
     CDS             17223..17945
                     /locus_tag="PKH68_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX11711.1"
                     /translation="MKKLIVYVPVLLFCVFTVQLKAEENAVVSSDTPVLTDIQVIDQD
                     PNLSSSLVWDKNKSTKKEKKKKSDFGKQMWKNANFGWNTYKTDDYKWRNIFIGTGYGR
                     DFENAKDAYWQVGLNLNWSKYTLYPEGKFNFTDNKDYLKTTSFSVPAIAGYNIYKSKL
                     RSVVLKAYTGPVFEFIVNSKVNGYTQNLHPTNNFQTGWTVGANLRFLYLLSARVAYTY
                     YPYSLFQKDDLYRRALSFSLGF"
     gene            18112..18987
                     /locus_tag="PKH68_01875"
     CDS             18112..18987
                     /locus_tag="PKH68_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNX11712.1"
                     /translation="MLQSMTGYGKANCEINNKKIVVEVKSLNSKQLDLSTRIAGVYRE
                     KDIEIRNILSQRLERGKIDFSLYIDNSGKEGGSKINQAVVKSYYEQLKSLTADLEIPD
                     SQDWLAILMRMPDTLKTETEELDENEWAEILKTIELAVRQLIEYRIQEGKTMENVFNQ
                     KIGKIGELLVEIEPFENERVEKVKARLEENLQALSDKIDYDKNRLEQELIFYIEKLDV
                     NEEKVRLRNHLEYFINTMKTESAPGKKLGFIAQEIGREVNTLGSKSNHSEMQKIVVMM
                     KDELEQIKEQVLNTL"
     gene            18999..19565
                     /gene="gmk"
                     /locus_tag="PKH68_01880"
     CDS             18999..19565
                     /gene="gmk"
                     /locus_tag="PKH68_01880"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNX11713.1"
                     /translation="MKVKLIIFSAPSGAGKTTIVNRMLAKDLGLEFSISATSRKPRGK
                     EQHGREYYFLTEEEFRNKIATDEFIEYEEVYPGCFYGTLRSEIERISAQGKNILFDID
                     VKGGINIKNQYGGRALSLFIAPPSIETLRQRLIHRSTDSPEMIEKRVEKAQYEMSFAP
                     QFDMIIVNDKLDKAVEQAADAISTFISE"
     gene            19583..20188
                     /gene="nadD"
                     /locus_tag="PKH68_01885"
     CDS             19583..20188
                     /gene="nadD"
                     /locus_tag="PKH68_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNX11714.1"
                     /translation="MKTIGLFFGSFNPIHLGHTDLAEYIFQFSGVDEIWFVVSPRNPL
                     KEQHELIDEQLRLKMISLATADKDYLQVSDVEFDLPKPSYTINTLRVLNEKYPADDFI
                     LLIGSDNMQIFDQWKDYQIILNDYSVIVYPRKGYPYEDFEEKYPEMQVLEEAPFFEIS
                     STEIRQLIAEKKDASQWLHPEVYQFIIENELYTNNSSELLC"
     gene            complement(20330..>21029)
                     /locus_tag="PKH68_01890"
     CDS             complement(20330..>21029)
                     /locus_tag="PKH68_01890"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX11715.1"
                     /translation="TRGNVELVHPKLRIIFEDSDLIVVEKKEGLLTVTTGRESETTAF
                     LILKNYVKKSSTRNKIYTVHRLDRETSGVILFAKHSDAQHILRDNWHEIVTKRVYVAV
                     VEGKVEKESDRIVSWLTENEISLKISSSRTDNGGKEAITNYRVIKSNDEFSLLEIELE
                     TGRKNQIRVHMESIGHPIVGDRKYGSTTTIGRLALHARVLEFYHPMTSEPLHFETPVP
                     REFLKVFRNQVEKK"
     assembly_gap    21030..21129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21130..21402)
                     /locus_tag="PKH68_01895"
     CDS             complement(<21130..21402)
                     /locus_tag="PKH68_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNX11716.1"
                     /translation="MRKNTNYTPPPKMTRLKVSEATQLMDFLLAKMGGMSRSSVKSLL
                     GHRQVTVNEKVVTQFDYMLKAADSVVINSTRGNVELVHPKLRIIFED"
     gene            complement(21421..22194)
                     /locus_tag="PKH68_01900"
     CDS             complement(21421..22194)
                     /locus_tag="PKH68_01900"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HNX11717.1"
                     /translation="MLLKTFIKLYKKPLILASGLILGTVLFILISEQCKHHQEEHMKS
                     KVITDCDRSLYATAPIAPTRKLKDQNDVQLLHAQANGLKNVYISNASFEADSARLAKE
                     QILIHLHDNPSYRVKEMTHSYPYTTPEMGDLLNDINVIFQEKMKDTKQDKYLFRVTSG
                     LRTNETQQELTSRNSNASTQSAHLFGATLDVSYKEFYNVTKDTIEANFYAAEALRETM
                     EDLREQCRMVIVRERRQACYHFTVVVCAPTNSADAVKTN"
     gene            complement(22347..22733)
                     /locus_tag="PKH68_01905"
     CDS             complement(22347..22733)
                     /locus_tag="PKH68_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNX11718.1"
                     /translation="MEISGKIIAVLPQATGQGKNGMWRSQDYVLETSTSGEQYPRKVC
                     FNLFNDKIDKYPLAIDDVVTVSFDIESREYNGRWYTSIKAWKIEKNAVNATPAASQAA
                     SVNEPATSFTPFEAGNGAENGSDLPF"
BASE COUNT         6466 a         4758 c         4897 g         6764 t
ORIGIN      
        1 cagtgatttt ggggtagttg cctgaaattc cttcagataa aaaggctcat agtatgccac
       61 atcagcaaaa tcgccggctt caaattttct ttcggctaat gcaatcatat ttttagccaa
      121 tgggtgaatg tcgatcagaa aatgggcatt ggggtgggtg agtgttgccg cacattttgc
      181 cgcaccattt ccgaagaaat aaaccggatt ttccgccagt aaattatcaa acgacccttc
      241 agtgatgatt tcagctgaaa tttcctgttt tacttccagc tttggggtgt aaaaggcggt
      301 atatacttcc atcctccgtg catctatcat ggggcaaaga agcacatttt ctttgttttc
      361 caactttttc aatgcttcga cacagagcac ttcgagcgta ctcaccgcta tcaacggaat
      421 attaaatccg tagcacaatc ctttggcggt ggatacccca attcgcaatc cggtatagga
      481 accgggtcct ccgcttactg caacggcatc aagtggcaga tttagttcct ttagtttatc
      541 cagtgcctgt tgcacgaaaa gacttagcag tcgggcatga ttagaacctt cgctgttact
      601 gatttcaaaa atacagtcgc catcgtggct taacgccact gagcaaacat gggtggaggt
      661 ttctatatga agaatagtca taaaatagtt ttgtgcgtga aataaatgat gaggatttgg
      721 cttactatcg attttttcaa attttcagca acgtatcatt aatctttttt gcctgtaagt
      781 tgaaagtaat atcccaggct tacggagaca ttcagaaaat tggatgcttt gaaataatct
      841 gtcttggcat cggcaaaaat attgcttagt ccatagtaag ctcttgtttc caactgaaat
      901 acaccagcct ttcgggtatg caggttatat ccaaatccgg cagcaatacc gtagtcaaag
      961 ggattgtaaa tgggagtttt ctgttgttct tgtggttcgg caacggtatt tatcagatca
     1021 gtttctttga gtaaataact cagtttggga ccgagattaa atataaaacg attcgttttt
     1081 ccccagtata agtgtgtcaa caacggaaat tccacataat ccgcccttcg agaataattc
     1141 ccgccggctt cttcccagcc ccgctgtgaa tagttggcct ctatctgcaa accgcaatga
     1201 ctttcggtaa catagcggat cgacagtccc ccgttgtatg ccagcagtgg catggactga
     1261 ttgacagtag gattgaacat aaccatcgat ccggtcatcc ctccggttgc tccgatatat
     1321 atttcgggtg gatcgagtaa tacagtttgc gccctgaggg caggaaaagg gataagcaat
     1381 aacaatccgg gcaagaggat gttgaatatc tgtttattta cgttttggag catcttcaat
     1441 taaatatagc tgttgattga taaaaccgcc accgtcgcgg gtgcgtttga aattgtaatc
     1501 ggactgtatg ccctgatcaa acttcacaga acttgtcgtt tcgttgaacc ggaaaccgtt
     1561 gttggtcatt tgtttgacaa aaccattggt tatatcgtat ccgagcatat cgaaacgtgg
     1621 atttttctcc gacctgaact gtttgtagaa aagtcggtaa tcattttcgt actgaatttt
     1681 ttcaggtgaa gcatcgttgt gatcgaaagg tgcaatgtag tacattttcg gttttttccc
     1741 gtttttgccc cgccaggaat actgtcccag tacacttaca tcgttgctgc tgcttaattc
     1801 atacaatttg ttcagataag tttgtacgga actcaaatct tccgtgtcaa aaacaatgac
     1861 gtttctttta gccgtcgaaa gattggtttc aatcgtttta acggagagaa tatcagcagc
     1921 tattttggtg taagatacgt tgagtttgtc aagtttcttt tgcaaaaacc ggaagaaatc
     1981 tgttccgtct tccgaccatt ttgtattgcc ggtttgagca aaaacaatat tggcatcgct
     2041 gaatttattt ttgagtacag cagctgcata atcgttgtgt atatcgttgt cgggattgaa
     2101 ctggaataca tacggattgg tttcaatatt ttttatactg gaactgaaag gtatgactgt
     2161 gggtattttt cttcggttcg aaaaatcaat caataccggt atttgcgccg tataagccgg
     2221 gccgatgatc agatccattg tctgcatttc aggcttattg agtacttcgt tgatttttac
     2281 ttcgctcttt tcggtgtcgt aggtatatac atcaattttg aaatttcctc tttttgcatt
     2341 gttgatggcc aggagtgagc ccatgtagaa ttcaagaaat ttttctactg ttgggtcctg
     2401 tttttcgcca tcgagcataa aaggaagcaa gtaggctatc ttgtaagaag ttttctcagt
     2461 aaccggtttt aatttcacct caccgtttcc atttacggtt gtccgggtgt tgtaggggat
     2521 tttaagttcg gttccggtct tgagtgtagt ggccgactcg ggatttaacc gtacgatgtc
     2581 atccaccgct acattatata ttttacttat cgaataaagg gtttccttga tttctacccg
     2641 gtgaataaaa tacgtctcat tggttccgat atgcagtgta gcaggaacag gagcggattt
     2701 tgcagtcgtg gattccactg gttttggtaa tttatcagcc tttttttcct ccttttctac
     2761 ttttggcttt accgaagtat cctgatttct tcttttcacc gggatgcgca gtacatcgcc
     2821 cgttttcagt ccgttggcta tctgtggatt agcttcacgg atatcttcct gactcacatc
     2881 gtacttgcgg ctgatggcaa aaagggtttg ttttttctct accgtatgaa gtataaaccc
     2941 atctttatca ttagaattat tcgtttcggg ctgaatttgt tttccggttt tcggaatcag
     3001 tatctcctgg ccggctttaa tcccgtcgtg aatttgggga ttggcattgt taatgtcggc
     3061 ctgcgataca ccgaattttt tgctgatggt atataaccct tcacccgctt ccacctgata
     3121 aatgtaatac tccgtaccat caataacttt ggtggagtaa accggcttct gcgcatatat
     3181 tattgctgag cagagagtta gtattatcaa taaggttaca ctgattttta gtttcatagt
     3241 gttatatatg aattaaaatg atcgttcaat ttccgcgatt aatttgcaaa tgtacaaaaa
     3301 atatagattg agaggctaca aacaacaata aatcagccgt ttttttgttt atcaacataa
     3361 aaaagatgta tttttgcaac aggaatgcat acagaactga aatttcagat ttcgtatttt
     3421 cagttgcaaa tatgtagctg atgtattatt acaaaaacca caccaacccc cgacagacat
     3481 gaaacgaact tttctatttt tcacactgct tttattcact gtgatttcat tttcgcaaat
     3541 tcaggtcagg ggaaattata ccagctacga tgaagcctcc ggattggacc acatttatat
     3601 tttttcggtt attgacaatt cttccgagat tcattacacc agtaccggta atacggcttt
     3661 ggttaaatgg ttcacttttc tcaacggtgt gaaaaccgaa ctcaccagcg tatcaatagt
     3721 atcacccacc gaaacatata tcgatcccaa aaataataca ggttacatca tcaatgtcaa
     3781 cggcacggat attgcccgat tttgggtgtt cgattattcg cagtatattc ccgttttcac
     3841 ttcactgaat ggtactgatg gcgaatttcc ctgcgaaaat gtagcgttgc aacttgtagg
     3901 caatgtgccg gagtttaagt atcaaaatac cggtggaggt gttttcacaa tagatcgtta
     3961 ctttaatatt gaatacaata cgctggagtg ggataccgaa aaaaaggaat ggaaagtaaa
     4021 aattcaaaca gaagagatta aacaacccgg atcggtaata ccgttgccaa cgccatcaac
     4081 caatactact tttacgctga agggcgatca gtttgcatcg cagctcaaca tgcctgaagc
     4141 atcggttact tccggtgagt acgtaaccaa agctgtaaaa tgcaagataa caacggtaac
     4201 ttcggtacgc gaggcaaaga atgagaacga ccgtccggag aaagaaaccg atattgaagg
     4261 ttcggcaccg ctcgatattc aattctacag caatcccact cccaatgtgc gaagttattt
     4321 ctggactatt taccgcaacg gcgagaaact catggcgcgc tccgaaaagg atcatttata
     4381 cactttttct aaagctggaa attacaaaat agaactgagt gcaaccaatg gtatctgtac
     4441 cgattcatcc tcggtcaatg tagtggtgat ggaatcgtcg atcattgctc caagcgtgtt
     4501 cactcccaac aacgatgagt ataacgagga atttcgggtg gcataccgct ctatcattga
     4561 gtttgcctgc accatataca accgctgggg tagggtggtt tacaagtgga ccgacccggc
     4621 caaaggctgg aatggcacca ttggcggaaa acctgcagcc gaaggaactt atttttacat
     4681 catcaatgcc cgcggatcgg acgataaggt gtataaactg aaaggacatc tnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttattttac aatcaccgct tccaggtttt
     4861 tatttctgat ttgaatgaaa atgggttctc cgtttttgaa gtacggtgca ttcacataac
     4921 ccattccaat cccttttttg ctatcgggga gcatggtacc ggaggttaca tgtccgatta
     4981 tagcaccttc gccattcaca atatcatatc cgtgacgcgg aattcctctt tcagttaatt
     5041 caaagcgtac gagcttgcgg gcggtgcctt cggctttttg cttttcgagc agtgcacgat
     5101 ccgtaaaatc ttttccctct gcaaattttg tgatccatcc cagtccggct tcaatgggtg
     5161 aagtggtgtc gtcgatgtca tttccgtaca ggcagaaacc tttttccagt cgcagggtat
     5221 cacgtgctcc cagcccgata ggtttaatgt catattcttt tccggcttcg aataaagcat
     5281 tccagatttg tactccgtgt tccggataaa aatagagttc aaatccacct gcaccggtat
     5341 atcctgtgtt ggaaagaatg acattggcca cgcctgcaaa ttctcctacc gtaaaatgat
     5401 agtaaggcac ttccgacaga ttgaccgggg taagtttttg cagcagttct gcagctttgg
     5461 gtccctgtac agcaagctga gcggtgtggt cggatgcatt ttcgagtacg gcgccaaccg
     5521 tattgttgtc gttgagccag ttccagtctt tttccacgtt ggaggcattt accaccagca
     5581 tatatttttc gggttcgaaa taataaacca gaatatcatc cacgatgccg cctttgccat
     5641 tgggaaaaca ggaatattgt gcctgtccaa cattcagttt tgaggcatcg ttggagcata
     5701 atttttgtat cagatcaagt gctttcggac cttttaccca tatttcaccc atgtgcgata
     5761 cgtcgaaaac gcctactccg gtgcgaacgg tcagatgttc gtcattgata ccactgtatt
     5821 cgataggcat attatagcct gcaaattcgt gcatcttggc acccaatgca atgtgaatgt
     5881 ctgtaaatgg tgtacttttc atagttttgt gttccctttg ttttattgcc aagggagtaa
     5941 attaagatta ttattgattg tgattagttt atattaaaag caaagtattg ttcttatgtt
     6001 ttttgcgttt atcaggattt ctgttcgagc agttcggcta ttttcactat taccatcatg
     6061 gctttttcca tggaagggat gggcagatat tcaaatcgtc cgtggaagtt gtgaccgccg
     6121 gcaaagatat tcgggcaggg tagtccttcg aatgaaagcc gggcaccatc ggtgccgccg
     6181 cgtatgggtt tcacattggg ttttacgcct gtggcattca tggcttcgaa tgcaagatcc
     6241 accacgtgca tcaccggctc cactttttcg cgcatattgt aatactgatc gcgcaattcc
     6301 accacagcgg tattttcacc atattcggca tttattttat tcgccagatg ttgtatttct
     6361 tttttccgac gctcaaaccg atcgcggtca tgatcgcgga tgatataggt gagcagggtt
     6421 ttttccaccg ttccttctat atttgtgagg tgataaaatc cttcgtaacc ttcggtatgc
     6481 tcgggcgttt cgtggcgtgg aagcatgctc acaaactggg tggcaatacg catggagttt
     6541 ttcattttgt gtcgggcgta gccgggatgt acatttagac cggtgatggt aacctttgcc
     6601 gatgcggcat tgaaattttc aaattccagt tcgcctattt cacctccatc catcgtataa
     6661 gcccactcag catcaaattt tttgacatcg aaatgatcgg ctccttgtcc aatttcttca
     6721 tcgggagtga atccaacacg aatttttccg tgtttaattt ccggatgttg gatcaggtac
     6781 tccatagcag taacaatttc ggccagtccg gctttgtcgt cagctccgag cagtgtggtt
     6841 ccatcggtta ctattatatc ctgaccttta tattgcagta tttcgggata tttttcagtt
     6901 tcaaaaacga tgagtttttc ttcgttgagc aggatatcgc cgccgtcgta attttctaca
     6961 atgcgtggtt ttacattttt gccgctggag tcggggctgg tgtccatgtg tgatataaag
     7021 ccgatgacag gtacttcctt gtcggtattg gcaggcaatg tcgccattat atagccgttt
     7081 ttgtcaaggg ttatgtcttt catcccgata gatttcagtt cgtcggccag gtatttggca
     7141 aaaatcattt gtccgggtgt actcggcgtc atatttgtat tctcatccga agtagtggta
     7201 aaactcacgt acttcaaaaa acggtcggta atattcatga atatttttgg ttaatttggt
     7261 taataaatat ggagtacaaa tttataaatt ttatctgtat taaaacctga atgatggtat
     7321 tttgtttaat cacgacccaa aatattttgt tccaatcaga tttacagcat aatttccgat
     7381 gcaaatgttc gggtgatacc ctgcctgtaa tgcactgatg aatggccgaa tattaccacc
     7441 aagccttcac ggcttttata ttttatctga tatttttctc tgaattgccg ggctttttta
     7501 ccgtattgaa taaatggatg aatgcttcca atcatgcatg tgcccagtcc gagtgactca
     7561 gcggctatca tggcataagt ggctgctacg agcggatcgg cagggtcgct gtatggcgtt
     7621 ccgtaaaagt acatcaccaa cggtgcatcg taggtgagcc agtttttgcc ttcggcggtg
     7681 cttgtaacga aaagatcaat catcggagct atgaaagagg cgaacatttc agcatttgat
     7741 ttgctcataa atagtcgcat cagagccagt gccggttttg aggccatcca tttcatgctt
     7801 tcaagtaagt gcgagaagtc gttgacaaat tcggtgtttt tgtctttact gttaataacc
     7861 aatacattaa catcggatgg tggcaatcct atgggagctg tgcgggctgc tttaagtatc
     7921 ttctcggtta tcacgtcatc aatcggttga tccttgaatt cccggatgct tctccggtgt
     7981 tgcagcaggg ccagcacttg atcatagcct gccatacgct ctttatcggg aagatcaaac
     8041 aagtcagcgg gcgatgctgt tctgccgctg atggtgatgg ctccttccgg acaaatggcc
     8101 atacagtgac cacaggcaat gcatccaaat acatccgtat tgcttagaac tgcttttcca
     8161 ttttgaaggg tgatatcaaa atctttgcat accgaaacac aaagtccgca tccattgcat
     8221 ttttcgatat caatgctgat gttgcctctt tcgctggttc gtgaagttgg aatggccatt
     8281 gaatactcaa ttgaatgtta ataaacagtt tatttctaat taccgaaatt aattcagatg
     8341 atagccttgt tctgtcagtt ccaaagcagt gaaataccgg tgtttttcta catccagcgg
     8401 ataatcccaa caaccaagac tatgataaat ttcacctcca aaacccgttt tgaaacggta
     8461 aagaccatac aatgggtgat taggatcggg attgggtgaa actccaaaca tatcatactc
     8521 actgcagccg cttgcttttg aaatttttat tgcttcccat tgcagcgcat aggttgccat
     8581 cagatttctg ttttcggatg aagaagcacc ataaaggtat gttccacgat tgcctgttat
     8641 aaccagaaac atggctgcca atggctgatt attatattct gcaatcagca attttacttc
     8701 agcaggtgac cgtacatcat ttgttctggc agaaagtaca gctgtaaagt acttaatatc
     8761 atttactaaa atattatttc ttttagcagt ttccttatac aatttatacc agatatcaat
     8821 attctcgatg tcagatgatt tgactacaac gcctttttta taagctaatc cgatgttata
     8881 cctggttttg ggtttcatgt cagccaatat ttcttcgttt tttttcaaca aatcaacgta
     8941 aattgtattt gaaggtaaaa tattggacaa tgattttttg aaattccagt tgatggtgtt
     9001 gaaattaaac ctcatttcct gtgtcttcac ctccggttca cctttccagt gcccgaaatc
     9061 atcaaaataa tccttttctt tagcccagta tgactcccag cataagtcat atcttatcat
     9121 gatgcagttt ttaggaagaa aagaccggat acattccgaa agctcttcaa gaaataatcc
     9181 ctgaaattcg tcatccggtt caagttcggg gccataaggc acataagcaa tggagtgatt
     9241 ttgattaata gctctgacaa taaccagcaa atctgagacg atattatcgt ccttggattc
     9301 agaattatta tacaaaaacg atttgtttga tttgaaattg atggcaaggg tcgagacacc
     9361 gagttttttc ttaactatcg accagaaagc cgtctgctga acaatgggag tggtgaataa
     9421 ttgttctact tctttattga atatattact aagcatgatg caggcaaaga taacaacttt
     9481 aaatgaatca aaaaaatatc tgttgccaat tgactggaaa taagaaaagt aaaaaatccg
     9541 atttgaactg attattcaaa aaaaaacgca tacacttgcg atgaattaat gtctgaaatt
     9601 gcatttgaca atttaattac actgttttat cagcagtttg ttacaaaatt agaaaatttc
     9661 attatgtttg taacttatta tcaaccaatt tatgcggtcc ggatacctta cagttcttat
     9721 attattctta tcctgtaaat ttatttttgc aaacaatcca ctgccactcg acagcgtgct
     9781 gcaccaggct atgaatgccg cagaatcata cagcggtctg gtcagcgatt atgaaagcga
     9841 agtgtatatg cgcgcttatg ttaaaaccaa caaaaaaaat tttctttaca aatatactta
     9901 tcttattccc ggatttgtac ttcacgaccg aaagaatgac gaaggattga tagaatcaat
     9961 cagtaagctg agaatggcct cttccaatat ttattttcag gatgtcaatt atatcaatgg
    10021 cactatgacg cgccgtagcg acatagcaat gctgccgttc cggtttatga gtttaaatat
    10081 ttacaaggaa tcagctccgg atgaaagcta cctgctgccc ttgagaaaaa cttcagccgg
    10141 atattaccag tacaaactga taagcgtaga aaccgccgat cacaaaccgg aatacaccat
    10201 tgaatacacc ccccgttaca aaaactccaa acttatcagt ggcacttttg tcctcgaaga
    10261 cagcacctgg cgtgtaaaac attttaccgg caagggaagt gatctctttt tcaatttttc
    10321 attcgaaatt acaatgggtc aggatctcat aaacagttat ctgcccgtta aatttgccat
    10381 acggcaagcc tatacttatt ttggcaataa ggttgtcaac cggtacaatg ccattctcac
    10441 ctataaaaat gtgaaattga aagaacagat cgaaaccaga aaaaatctca atttgggtaa
    10501 tcggtataag gtgcgtatgg attcggtgcc tgcgaatgtt gacagcacgt tttggaataa
    10561 aattcgacct ttaccgctgt cggagaatga acaattactg ttgagtaaat acgccgaaaa
    10621 acgaaaaaaa actcaaaccg aacaacaaaa ggaagatact acaggcataa aatcagccac
    10681 agcactcaag tttgccaaaa atatggtgtt ggacagcaag tacaggtaca agtccacaag
    10741 tatccggtac agtggtgttt tcaatccctc catgatcagt tattcttccg aagacggact
    10801 tacctatcgg caaaaattgt ttctggggat tgaactggca cgccatcagc gcataaatat
    10861 agatgcattt gccggctatg tgttcaataa aaaagagttt tacggcgatt tatcagcggt
    10921 gtggaattat gatccggcta agtttggaaa tctctctttt tcaatcggca aaggtaacca
    10981 atcctatagc tcgctattcc tcaatatggt aaaagatagc ctcaccagcc agggattgaa
    11041 tatcgaagac ctgggagtga attatttcaa ggatttttac gtccgggttt tcaatagcgt
    11101 agaaatcaca aacggattgc agctcggcgc cggcttcgat taccacatac gggaaccaaa
    11161 caagtcacaa atgagcgcat caggccccaa tcacgagcag atggatggaa tttttgacac
    11221 ccggtattat tttgttccgg ttatcagtct tagctggaca cccgagcagt attacaccat
    11281 ggacaaaaat cagaaagtgt acgttcgctc cgaatatccg acgttcaaaa ttcagtatgc
    11341 ccacagcctg aaaaacgtat ttggaagtac ttcgcagtat agccgggtgg aatttgacat
    11401 taatcagaat atccgcttcg atttgatgaa atcgcttcag tatcatgtgg gaacgggatt
    11461 tttctcccgt cagaaaacgg aatatttcac cgattttgtg ttttttcgcc ggagctactt
    11521 ccccgaaaca tggggagagg gaataggagg ggtgttcaat gtgctgcccc gaaatttgta
    11581 taatgcttcc tcatcctata tacaggctca cctgatgtat gaaaccccgc gatttttgct
    11641 cacccgaatt caggcgttat cgggtggtgt tgttaaggaa agactttatt acagccagct
    11701 gtttacgcca accatccctt cgtacagtga attgggctat ggggtcggca accgtttttt
    11761 gaacgcagcc cttttcgtgt cattctttaa actgagttac cagaacatcg gtttcaaatc
    11821 tgttttttta ctttaaagcc gctgtctatt atcagaaaca atgcctatct ttgtgcctga
    11881 tattcagaat gtacataata tgcagcagat aacccaactt accgattttt tacccactac
    11941 caaaaaagaa ctcgaactgc gcggatggga ggaggtggac atcatcctgt tcagcggcga
    12001 tgcttacgtg gatcatccct cgttcgggtc ggccgtcatc ggacgtatgc tggaagcgga
    12061 ggggttgcgc gtggcactca ttcctcagcc caactggcag gacgatttgc gtgatttccg
    12121 caaactggga cgcccccggt tgttttttgc tgtgagtgcg ggcaatatgg acagcatggt
    12181 gaatcactat acagccaaca agcgattgcg ctctgacgat gcctattcac ccgacggacg
    12241 cgctcacatg cgacccgatt atgccaccat cacttattgt aacatcctga aaaaactgta
    12301 tcccgatgtt ccgatagtaa ttggtgggat tgaagcctca ctgaggcgcg ttacacacta
    12361 cgattactgg tccgacaaac tgatgccatc catcctggtg gatagcaaag ccgattttct
    12421 ggtatatgga atgggtgaga agcccttaag ggaacttatc agacaattat gggaaggaaa
    12481 aaaaatcggt gaaattacgg atattccgca aacctcatat attgccaacg aaattccgga
    12541 ggtggaaaat ttgcaaacaa ttgaattggt ttcacacgaa gaatgcctga aagacaaaaa
    12601 gaaatatgct tccaatttca ggtatgtgga ggaagaaagt aaccggatgc agggtgcacg
    12661 actggttcag aaagtgggtc gtaagcacat cattatcaat ccgatgtatc caccatggag
    12721 tccggaagaa gtggatgcta cttatcgctt accttttacc cgtttgccgc acccaaagta
    12781 caagggtaag accattcccg cctacgaaat gatcaaattt tcggttaatc tgcatcgggg
    12841 ttgctttggc ggatgtgcat tttgcaccat ttcggctcat cagggcaagt ttattgtttc
    12901 gcgcagcaag cagtccattc tcgatgaagt tcggcaaata accaaacagc ccgatttcaa
    12961 aggcaatctg agcgatttgg gcggaccttc ggccaatatg tacgccatgc agggcaggga
    13021 tctttcgatt tgcgtaaagt gtaaacgccc ctcatgcatt catcctgccg tttgtcctaa
    13081 cctcaatatc aatcactccg cattgaccga tatctaccgt tctgttgaca aaattccggg
    13141 cgtccgccat tcatacattg gcagcggcat acgttacgat atgctcactg cgcagtcacg
    13201 ggatgctgaa acatcccgaa gtcaacagga atatttgcgc gaacttatca caaaacacgt
    13261 ttcgggcaga ctgaaagtgg cacctgagca cacttccgaa cgggtgctga agctgatgcg
    13321 aaaacaaagt tttcaggatt tcaggaaatt caaggaattg tatgaccgta ttaatagcga
    13381 ggccggactc aatcagcagt tgatacctta tttcatatcc agccacccgg cctgtcacaa
    13441 agaagatatg gcagatcttg cagcgcaaac caaagacctg aattttcatc tggaacaggt
    13501 gcaggatttc acacctactc ccatgacgct tgctacagag atttattatt ctggctacaa
    13561 tccatacacc ctggagccgg tcttcaccgc caaaacaaag gaggaaaagc tggaacagcg
    13621 aaaattcttt ttctggtaca aaaaagagta tcgccagcag ataatgcgag atttacagaa
    13681 aatgaaccgc ccggatttaa tccggaaatt gtttggatag aaaaaataaa atggcagaaa
    13741 ctaattggta gggtggctta aagcaattta ttttactgaa taatttcacc attcactatc
    13801 tcaaaaatct gtgaactttg tcctatttgt gaaagtatat cgtccagatg ctttcggttg
    13861 gtatcggtga tgaaaatttg tccgaaatta tcgccggcta ccagttccac aatttgtttt
    13921 acccgcaggg catccagctt atcaaaaata tcgtcgagga gcagtatggg tgcaatgccg
    13981 tgcgtgcgtt tcagaaaatt gaattgcgcc agcttgagtg atatgagata ggttttgttc
    14041 tgtccctgtg aacctacccg tttcatcgga tagtcgccaa gcatcatttc cagttcatct
    14101 ttgtgtattc cattcatact aaagcctatc agtcggtcac gttcacgatt cgcctgaagt
    14161 ttttgcttca gatcagcgtc agtcaactgc gatttgtagt ttagatgtac ctgctcattg
    14221 tcgttggaaa tgaaacggta gatttcttga aaaatgggta tgaattcctc tacaaaatct
    14281 ttacgacgtg tataaatata gttgccgtac ttcaccatca tctcttccca tatttcgtac
    14341 agcgattcat cggtcagggc ttcctctttc aacagggcat tacgctgttt tagcgcattg
    14401 ttgtagttca gcagcgagtt cagataggtt ttatcgtatt gagatatcac gctgtccatg
    14461 aatttacgtc gttcgtcgct gccgtcagct atcagttcat cgtcacccgg cgaaaccagc
    14521 accaggggca gcaatccgat atgatcggcc agccggtcgt attccttttt atttcgtttg
    14581 aaatgttttt tctgcctccg tttcatgccg cagtatattt cctcttctgt atcgccaagt
    14641 gtatatttcc cctgcagcat aaagaaatcg gcattgtgtt caatattctg tgagtcaatc
    14701 gaattagaat aacttttaca gaacgaaagg taataaatag catccaacag gttggttttg
    14761 cccatgccgt tattacccac aaaacaattg attttcgaag gaaattcaag gtcggcctga
    14821 catatattct tataattcag tatggatatg gaattcagac gcatgttgct tgtaatcggt
    14881 tatggatgtt tttttttgaa aatagttcat gcaaaaatag aaaaaaaatc gctaaatttg
    14941 tgttttattc tgatggatgt cattcatcag tttttagata aaggaaaata tacttttttt
    15001 cttttataaa tgtcatcctt tttttcattt tcaaaacaaa aaaaaacagc aattctcaat
    15061 aataaatatg gtttcagagg aattaatcaa acagctcacc caatccactt cgtattcatt
    15121 tcatcaggtt cagaatgtcg tccggttgat tgacgaaggc gctaccattc cgtttatgag
    15181 tcgctaccgc aaggaagcta ccggtgggat ggacgaagtg caactggctg atctcaggga
    15241 gcaatacgac aaactgtgtg agctgatcaa acgaaaagca acggtattaa aatcgatcga
    15301 ggagcaggaa aaacttaccc ccgaacttcg gcaaaggata gaggaatgct ggaatgccaa
    15361 tgaactggaa gatatatatc tgccctataa acccaaacgg cgcacgcgtg ccgaaatagc
    15421 gcgagaaaaa gggctggaac cgcttgccaa aataatcatg aaacaacaaa cggttgacat
    15481 tcaaaatgtt gttaataggt ttattaccag cgatgtaatg aatgcttccg atgctctgca
    15541 aggtgcccgg gatattatag ccgaatgggt caatgagagt gaagctgccc gaaacgccat
    15601 ccgttcgata ttttcgcatg aagcaatgat ttcatccaaa atggtcaaag gaaaagagga
    15661 agaggcacag aaataccgcg attattttga ttgttcggag aaactgtcac gctgctcttc
    15721 acaccgcctg ctggccatgc gccggggcga atcggaagga tttctgaggg taaatatcac
    15781 tgccgacgac gataaatgca ttgcccggtt gctcaggatt tttcagaaat cggacaatga
    15841 atgtgggctg caggttgccg aagctgtgga agatggctat aaacgcctgc tcaaacctgc
    15901 catcgaaacg gagtttgcag ccgacagcaa atcaaaagcc gatgtggaag caatccgtgt
    15961 ttttgccgag aacctgcgtc aattgcttct ggcacctcca ctcgggcaaa aaagggtgat
    16021 gggtgttgat cccggtttca gatcgggctg taaaatcgtc tgcctcgatg cacagggcaa
    16081 cctctgtcat aacgagaata tttacccaca cactccacag aatgaacatt cgcaggctaa
    16141 acgcaagctg caaaaaatgg tggaagccta tgatatccag gctattgcta ttgggaatgg
    16201 tactgcaagc cgtgagacgg agcgtttcat tcaaagtatc agcttcgacc gtaaggttca
    16261 gctctttgtg gtgagcgaga acggagcatc agtttattcc gcttcgaaag ttgcacgtga
    16321 tgaatttccg gaatatgatg tcacggttcg cggtgcagtt tcaatagggc gtcggctcat
    16381 ggatccactt gccgaactgg ttaaaattga ccccaaatca atcggtgtag ggcaatatca
    16441 gcacgatgtg gatcagacaa aattgaaaaa ttcactcgat agaacagtgg agtttgccgt
    16501 gaacagggtg ggagtgaacc tcaacacagc cagcaagcat ctgctgacct acgtttcggg
    16561 acttggtcct cagattgccc aaaatatagt gaatttcagg gctgaaaacg gcgctttccg
    16621 aaaccggaaa gaactgatga aagttcccaa aatgggtgcg aaaacctttg aacaatgtgc
    16681 gggtttcctc cgaattcctg atggtgataa tgcactcgat aattcggccg tgcacccgga
    16741 aagctatcct gttgtcaatc agattgctac agacctgaaa ctgaaagtaa acgaactgat
    16801 aggaaataat aagcttaaat ctacgttaga tttatcgaaa tatgtgacag aaaaagtcgg
    16861 aatccccacg ttgaacgata ttctggatga actcgaaaaa ccgggacgtg atccacgttc
    16921 ggcaatccgg atttttgagt ttgatcccaa tgtgaaagat atcaatgact tacgtacagg
    16981 aatggaattg ccgggcatca tcacgaatgt tacaaacttt ggcgcgtttt gtgatatagg
    17041 aattaaagaa aacggattaa tccacatttc caatctttct gacagttttg tgagcaatcc
    17101 agccgacatc gtttcggttc atcagcatgt aaaagtgagc gtactcgaag tggatttagc
    17161 ccgtaaacgg atacagttaa aattgattta aacgagaata aattacctgt aaattattaa
    17221 atatgaagaa gttaattgtt tatgttcctg ttttattatt ttgtgtattt accgttcaac
    17281 tgaaagccga agaaaacgca gttgtatcct cagatacacc tgtactcacc gatattcagg
    17341 tcattgatca ggacccgaat ttaagcagca gtctggtttg ggataaaaat aaaagcacta
    17401 agaaggagaa aaagaaaaaa tccgactttg gaaaacaaat gtggaaaaat gcaaattttg
    17461 ggtggaatac ctataaaacc gatgattata aatggagaaa tatctttatt ggaaccggtt
    17521 acggccgtga ttttgaaaat gcaaaggatg cctactggca ggtggggctg aacctcaact
    17581 ggtcgaaata taccctttat cccgagggaa aatttaattt tacagacaac aaagattatt
    17641 tgaaaactac ttcgttctct gttccggcta ttgcgggata caatatttat aaatcaaaac
    17701 tgcggtcggt ggttctcaaa gcctatactg gtcctgtatt tgagtttatt gtcaattcca
    17761 aagtgaacgg ttacacccaa aatctgcatc ccacaaataa ttttcaaacc ggatggacag
    17821 taggtgccaa tctgcgtttc ctctacctgc tgagtgcacg cgtggcttat acctattatc
    17881 cctacagctt atttcagaaa gatgatttat accgcagggc attgagtttc tccctgggtt
    17941 tctgattagc taacttgtat aaaagccgga aatgaattcc ggttttttta tttctgataa
    18001 tttctctcaa tttcggataa cagaaaattc ctaaatgcaa ttcgggtata tgggttcaca
    18061 aataatttaa gtacttttgc acaaaataat tctcaaaaaa aaacatcgta catgctacaa
    18121 tcaatgaccg gctacggaaa agccaactgt gaaattaata ataaaaaaat tgttgtggag
    18181 gtgaagtcac ttaacagcaa acagttagat ttatcgacac gcattgccgg cgtataccgc
    18241 gagaaagaca tcgaaatacg taatatactc agtcagcgcc ttgagcgtgg gaaaattgac
    18301 ttctccctgt acattgacaa ttccggcaaa gaaggtggct caaaaataaa tcaggcagtg
    18361 gttaaatctt actacgaaca actcaaatca cttactgccg atttagaaat tccggattcg
    18421 caggactggc tcgccatact catgagaatg cccgataccc tcaaaaccga aaccgaagaa
    18481 ctggacgaaa atgagtgggc tgaaatactg aaaaccatag aactggcagt cagacagctg
    18541 attgaatacc gcatacagga aggtaaaacg atggaaaatg tatttaacca aaaaatagga
    18601 aaaatagggg agttgctggt ggagatagaa ccatttgaaa atgagcgtgt tgaaaaagta
    18661 aaagcccggt tggaagaaaa cctgcaggcc ttatcggata agatagacta cgataaaaac
    18721 cgcctggaac aggaattgat tttctacatc gaaaaactgg atgtgaatga agaaaaagta
    18781 cggctgcgaa atcacctcga atacttcatc aatacaatga aaactgaatc ggcacccgga
    18841 aaaaaactgg gatttatagc ccaggaaata ggtcgtgagg taaatacact cggttcaaaa
    18901 tccaatcaca gcgaaatgca gaaaatagtg gtgatgatga aagatgaact ggaacaaatc
    18961 aaggaacagg tactgaatac attgtaatca tatacagcat gaaggtaaaa cttatcatat
    19021 tctcagcacc atcaggggca ggtaaaacca ccattgtcaa ccgaatgctg gccaaagacc
    19081 tggggcttga attttccata tctgctactt cgcgcaaacc acgcggcaag gagcaacatg
    19141 gaagggagta ttattttctg accgaagaag aatttaggaa taaaatagca accgatgaat
    19201 ttatcgaata cgaagaagtt tatcctggtt gtttttatgg aacacttcgc agcgaaatag
    19261 aacgtatttc agctcaggga aagaatatcc tgtttgatat tgacgtaaaa gggggcatca
    19321 atatcaagaa tcaatatgga ggccgggctt tatcgctgtt tattgctcct ccatccatcg
    19381 aaacactccg ccagcgtctc atccaccgca gtaccgatag ccccgaaatg attgaaaaaa
    19441 gggtggaaaa agcacaatac gagatgagtt ttgctccaca gtttgacatg atcatagtta
    19501 atgataagct ggataaagct gtagaacagg cggcagatgc catttcaacc tttatatcag
    19561 aataataaca acagactttc agatgaagac aatcggttta tttttcggtt cctttaatcc
    19621 tatccattta ggacacacag atttagcaga atatattttt caattttcgg gtgttgatga
    19681 aatctggttc gttgtgagcc cgcgaaatcc acttaaagag cagcatgagc ttattgatga
    19741 acaattgcga ttgaaaatga tcagtctggc tacagccgat aaggattatc tgcaggtatc
    19801 ggatgtcgaa tttgaccttc caaaaccatc ctacactatc aataccctcc gggtgttgaa
    19861 tgaaaaatat cccgcagatg attttatcct ccttattggc agtgacaata tgcagatttt
    19921 cgatcagtgg aaagattatc agatcattct gaacgattat tcggtgattg tatatccgcg
    19981 caagggctac ccttacgaag attttgagga aaaatatccc gaaatgcaag tattggaaga
    20041 agcacctttc tttgaaattt catccactga aatccgtcag ctgatagccg aaaaaaagga
    20101 tgcaagccag tggttgcatc ctgaggtgta tcagtttatt attgaaaatg aactttatac
    20161 taacaattcc tctgagttgt tgtgctgatg gcggcttttt gcttctgata aattcgggtt
    20221 atgcaccggt aagctcgcca gagtttttca gcaggtccta cttttataaa accaaaaagg
    20281 tttgcacaac tggtttcaga ttggcaaacc ttttttttat gatgtaaaat tatttctttt
    20341 caacctggtt tcggaaaact ttgagaaatt ccctgggtac cggtgtttca aagtgtaggg
    20401 gctcgctggt catgggatga taaaattcca gtacacgggc atgcagtgcc agtcggccga
    20461 ttgtggttgt ggagccatat ttccggtcgc ccacaatggg atgcccgatg ctttccatgt
    20521 gtacacgtat ctggtttttg cgcccggttt ccagttcaat ttcgagcagt gaaaattcat
    20581 cgttgctttt tatcaccctg taattggtga tggcttcttt gccgccgttg tcggtgcggc
    20641 tggaactgat tttcagcgaa atttcatttt cggtgagcca gctcactatc cggtcgcttt
    20701 ccttttccac tttgccttcc accacggcca catacacccg tttggtaaca atttcatgcc
    20761 agttatcgcg cagaatgtgt tgagcatcgc tgtgtttggc aaataatatt acgccggagg
    20821 tttcacggtc caggcggtga acggtatata ttttatttct tgtagaactt ttcttcacgt
    20881 aatttttcaa aatcagaaaa gccgttgttt ctgattctct gccggtggta accgttagta
    20941 aaccttcttt tttttccacc actatcagat cgctgtcctc aaaaatgatg cgcagcttgg
    21001 gatgaaccag ctccacattg ccccgggtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnng tcctcaaaaa tgatgcgcag cttgggatga accagctcca cattgccccg
    21181 ggtgctgttg ataaccacgc tgtcggcagc cttaagcata taatcaaact gggtaaccac
    21241 tttttcgttc accgttacct gacgatggcc gagcaacgat ttgacagaac ttcggctcat
    21301 tccgcccatt tttgccaaca gaaaatccat caactgtgtg gcttcgctca ctttgaggcg
    21361 ggtcattttg ggcgggggag tgtaatttgt atttttacgc atggctttat cggtttttta
    21421 ttagttggtt ttaacggcat cagcactatt ggtaggtgca cacactacca cggtaaagtg
    21481 atagcaggct tgccggcgtt cacgcactat caccatgcgg cattgctctc tgagatcttc
    21541 catagtttcc cgtagtgctt cggcggcata aaaattagct tcaatggtat cttttgttac
    21601 gttgtaaaat tccttgtacg aaacatcgag cgtagctccg aacaggtgag ccgattgtgt
    21661 gcttgcattg ctgttacgtg acgtgagttc ctgttgtgtt tcattggttc tcagtccgga
    21721 agttacccga aacaaatatt tatcctgctt cgtgtccttc attttttcct gaaaaataac
    21781 gttgatatca ttcagtaaat cacccatttc gggggtggtg tacggatagg aatgggtcat
    21841 ttccttgacc cggtatgaag gattatcgtg caggtgtatc agtatctgtt ctttagccag
    21901 tctggcagag tctgcctcga aagaagcatt gcttatatat acatttttca gtccgttggc
    21961 ctgtgcatga agcagttgta catcattctg atctttcagc ttgcgggtag gagcaatggg
    22021 agcagtggca tacagcgatc ggtcgcaatc tgttatcact ttgcttttca tgtgttcctc
    22081 ctgatgatgt ttacattgtt cggatataag aatgaataaa acggtaccga gaattaagcc
    22141 tgaagcaaga atgagtggtt ttttatatag tttgatgaaa gtctttaaca gcatgttttg
    22201 aaaattttca caaatgtact tcttttcgta caaccgacaa ttagccggtt gtaccttttg
    22261 caaattttag gttttatacg tttcggttaa aaaaaaagaa gcgggaacat tctcccgctt
    22321 cttgtcgtta tctgatgtaa taatgatcag aaaggtaaat ccgagccgtt ttcagcaccg
    22381 ttgcccgctt caaagggtgt aaagctggtt gcaggttcgt tcacgcttgc agcttgtgaa
    22441 gcagcgggtg tggcatttac tgcattcttc tctattttcc aggctttgat ggaagtgtac
    22501 cagcgaccgt tgtattcgcg gctttcgatg tcaaaactaa ccgtcaccac atcgtctatt
    22561 gccagtggat atttatctat tttgtcatta aaaagattaa aacaaacctt gcggggatat
    22621 tgctcaccgc tggtactggt ttccaacaca taatcctgtg aacgccacat tccattcttt
    22681 ccctgtccgg tagcctgggg caatactgca ataatttttc ctgaaatttc cataattcta
    22741 taattgttta atatgatttt ccgactgcaa agataattaa ctttgggaat gtataaaatt
    22801 tataatttga aaaagatgat tgaacaaaca aatttgccgg ttgttacaac aatatgaata
    22861 atcgtaaaat cattgataca tgaaacagcg atgccaaata tttttgactt acgtcttgtc
    22921 ccgaagtatt taggtcagcg aatgattgct ccagctcaac tattcataca aaactaattt
    22981 aacgctctaa atcccccctt taggggttag ggggcagata actaaatata aacatatgaa
    23041 aacaaatatg ggtatggccg acaaagccat acggctaatg ttagc
//
LOCUS       DAOPZK010000016        21556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2487_length_21556_cov_22.537510, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000016 DAOPZK010000000
VERSION     DAOPZK010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21556
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_21556_cov_22.537510"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKH68_01910"
     CDS             <1..268
                     /locus_tag="PKH68_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNX11719.1"
                     /translation="ILAEAAKSRDMIVNGAKDKAAEEAAKIMEAAREQIALEKEDAIR
                     QIRREVATLSVEISEKVLREKLDRKNSQMDMIDRLLDEINIPKS"
     gene            410..964
                     /locus_tag="PKH68_01915"
     CDS             410..964
                     /locus_tag="PKH68_01915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNX11720.1"
                     /translation="MNTGLIPVRYAKALLDFAHETKSVDKVYGEAKLITLMFMKMGEL
                     RLVLENPVIDQAEKRQLILNAAGGKTSKTFENFTDLLLKNNREAHIQNIMLKFVDLYR
                     VQKNIRYGKLTTATAVDESTEERLMALITDKVGGTLELEKVIEPAILGGFILEVDDVR
                     WDASISGQLTRIKNEFIERNRRIV"
     gene            1086..2666
                     /gene="atpA"
                     /locus_tag="PKH68_01920"
     CDS             1086..2666
                     /gene="atpA"
                     /locus_tag="PKH68_01920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNX11721.1"
                     /translation="MSDSIKVSEVSEVLRMQLEGIDTGVKFDEIGTVLQVSDGVVRIY
                     GLRNAEANELLEFDNGIKAIVMNLEEDNVGAVLLGPSSEIKEGYSVKRTKRVASIFVG
                     EGMLGRVINPLGEALDGRGKVTGELTELPLERKAPGVIYRQPVTQPLQTGLKAIDAMI
                     PIGRGQRELIIGDRQTGKTSIAIDTIINQRRNFDEGDPVYCIYVAIGQKGSTVASIVN
                     TLKEHNAMEYTIVVAANASDPAALQYYAPFAGAAIGEYFRDTGRHALVIYDDLSKQAV
                     AYREVSLILRRPSGREAYPGDIFYLHSRLLERAAKIINQEEVAKNMNDLPETLRSRVK
                     GGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLDTDLFNQGNRPAINVGISVSRV
                     GGTAQIKAMKKVAGTLKIDQAQFRELEAFTKFGGDMDPVTAMTIDKGQKNTRLLVQPL
                     HQPMPVEKQVAVLYCGTHGLLSKVPLNKVNDFEKEFLSTLELTHQEDVLDVIKKGIID
                     DTVTSILEKTAEEVARRI"
     gene            2805..3674
                     /locus_tag="PKH68_01925"
     CDS             2805..3674
                     /locus_tag="PKH68_01925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNX11722.1"
                     /translation="MASLKEIKSRIQSVKSTQKITSAMKMVSSAKLRKAQKRIENFYP
                     YERKLTHILNNFLSAETDNTSVYAENREVKRVAILVFSSNSSLCGGFNSNVAKRLHAV
                     MERYNHLGKENILIYPVGKKIAKVAKKMDFFVDNFDEMADKPTYAAAQDLADDLMELF
                     INHTIDKVELIYHHFNNKSSQILVDETLLPIRLEVNPEKQSGMQLNYIVEPDRNTIMN
                     DLIPKVLRLKTYTALIDSNASEHAARTIAMQTATDNADDLLQELNLQYNKSRQQAITN
                     ELLDIIGGSFGQK"
     gene            complement(3836..4918)
                     /locus_tag="PKH68_01930"
     CDS             complement(3836..4918)
                     /locus_tag="PKH68_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNX11723.1"
                     /translation="MNEVKVIEVFLNTVKVGRIALTSDALCAFEYDSSYLDTGKSISP
                     LILPLRKEVFIAKRNPFNGGFGVFDDSLPDGWGNLILDRYLKSKNINPDKLTLLQRLA
                     LVGSCGRGALEYRPDYSKSTPGDVLSLEFLAAETEKILKTKYTDTSLETLYNYGGSPG
                     GARPKVFVNMDDKEWLVKFKAVGDPPDIGRIEYQHSLLAKKCGIKMPETRLFEGRYFG
                     VERYDRTPQGKIHTISAAGLLNANYREPSLDYESLLKLCLLLTKNMEEVYALFRLMVF
                     NVAIKNRDDHAKNFSFQLINNEWKLSPAYDLLPSLGFNGFHTTTINNNGEPTENDMII
                     VAEKVGLSKSKAIQIIQEVNEIIKSG"
     gene            complement(4911..5189)
                     /locus_tag="PKH68_01935"
     CDS             complement(4911..5189)
                     /locus_tag="PKH68_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX11724.1"
                     /translation="MILNLSRRVKERRLELNLTQKSFSKRAGMGYDAYRRFENSGEIT
                     LKNLVLCAIALDSAEDFAGLFSRKSYQNMDELLKTQEVKKRLRGSRNE"
     assembly_gap    5199..5298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5624..6268)
                     /locus_tag="PKH68_01940"
     CDS             complement(5624..6268)
                     /locus_tag="PKH68_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11725.1"
                     /translation="MAANYSQIKPVIYDCKTCRTRCDGVTVPKFDFKHDVDFSEAVEN
                     EVTAIINKKYPGLFAVKTRQDGFPDIEVSRINSSSVCLYIEIKVQSRTFMSVQKYLPD
                     SGLHPSETLALNLSDLERYFRVKEREGVPVFITWCLLNRPCMVGTEPGSKRFFSQNLD
                     ELKKIRLKDKNDTRRFRRASGEGDVVDGVHKGVVINYHFSILELFEGLPDLSEL"
     gene            complement(6289..7380)
                     /locus_tag="PKH68_01945"
     CDS             complement(6289..7380)
                     /locus_tag="PKH68_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNX11726.1"
                     /translation="MTTLGTLELNNIHQGDCVELLKRIPDNSVDLIFADPPYNLQLNG
                     DLFRPDTSKVDAVDDEWDKFDSFAEYDQFTNQWLTECHRVLKPTGSIWVIGSYHNIFR
                     VGTALQNIGFWILNDIIWVKTNPMPNFKGTRFNNAHETLIWATKSKSSKFTFHYHSMK
                     VMNDDLQMRSDWVIPICQGNERIKVNGMKAHSTQKPEELLYRVLISSSNPGDIVLDPF
                     SGSGTTAAVAKLLGRNYIAFERDEFYISVARERLEKITTVQKEMTGYPIENRIPKVPF
                     NSLIVKDYVRVGECLYSKDGTISAVVNADGTLVSNGFVGSIHKVSAHILKKSNNNGWS
                     FWYVKRDKVLISIDALRYEYEEKYNKSVI"
     gene            complement(7508..10381)
                     /locus_tag="PKH68_01950"
     CDS             complement(7508..10381)
                     /locus_tag="PKH68_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HNX11727.1"
                     /translation="MKLSRLFSLILLCMSLFGAQAQQVTVSPIPQSITWGNKAFDNTT
                     TYVINGEATADKDAVALLKSKLSISATGVEILIGKNGDANVASVASEIPAVKEGYYLK
                     VEPGKVTIAGTDSVGTYYGVQTYLQIAAAPQVMSVTIKDFPNVIERGLVEGFYGNPFS
                     QADRIRQFEFYGQNKMNVYIYSPKDDPYHNSQWAVNYPNAQALKLKELVRKAHENKVK
                     FIWAIHLGGKGVTNTTEYAKVIAKFEDMYKIGVRDFAIFYDDFGTETDVQQCAATNYI
                     VQNFVNKKTDVGPITFCPTQYNRSWSSGTYLTTLGSQMDKSVRIMWTGNSVVDMINKS
                     DMDWINAQIGRKAYIWLNYPVTDYCIDHLLMGPTYGNDTNIAAQLGGFTANPMEYAEA
                     SKVALYSIADYCWNMTAYNPQASWLRGIKYLMPQNEAAFKIFCENNIDLGATAHGLRR
                     EGESAPFKAVADPFMTAYKAGTYSPVKADALAAQFQSFRDASAELIASTGSPEMIAEI
                     KPWLQVFDIIGAKGVSLVKMYKALNDKDSVAFIREYLHVDSLENAQKQIISRNFTGSI
                     KSPNPKPANEVVAPFIRQMKAMLVTEYKRNFNYMKNIFPAIILEEGKYFIKYNGAYLT
                     NKIGTTGNPTYVSTRDNINPQRQEWIITIDPLTERYKIVNAQDSRYVNELGNFGTNAY
                     DAAWNTYSLYRLNNRYAIQNGGSGGDKFWNSTTTRIGVSSINQIKVSNFIFEIIPVDN
                     DTITYPIIIPAETYYIKSGDKYLTKGATNTPVFSAQISPQSATQQWKLLVDKTTDRYK
                     LVYASDTTKYVNELGVIGTNSYYSTWNSYVVTEMGNKFAIQNAGDAGTKFWTITDNKI
                     SAGNATRQESYLFEIVPVNANTGLKKKIVNRYSFRLCKDVIQITGDNVSKIDLISING
                     STVRQSSGKENLMIEGLASGIYVLAVTSNKGQTETFKVLLGKR"
     gene            complement(10454..11287)
                     /locus_tag="PKH68_01955"
     CDS             complement(10454..11287)
                     /locus_tag="PKH68_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX11728.1"
                     /translation="MNLYRGCTHGCIYCDSRSKCYQINHTFEDVEVKSNAPELLEQAL
                     KRKRKKCMIGTGSMSDPYLPIESNLGLTRRCLEIIEENGFGLSILTKSNRIVRDLDVL
                     KKINQKSKCVVQMTLTTFDEDLCRIVEPNVSTTRERFETLKIMRDNGIPTIVWLCPIL
                     PFINDTEENLTGILNYCIEAGVKGILCFGFGVTLREGDREYYYSKLDEHFPGIKEKYQ
                     HKYGNAYEVMSENNPALMKLFLEICNTNHILSDTRQIFSYLSEFPVKNNDRQLDLFES
                     V"
     gene            complement(11360..11890)
                     /locus_tag="PKH68_01960"
     CDS             complement(11360..11890)
                     /locus_tag="PKH68_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNX11729.1"
                     /translation="MGIEIYIQLFISFFKIGLFGFGGGYAIISLIQHEIEIHGWMTQS
                     EFTDIIAISQMTPGPIGINSATYVGYTASGTILGSVIATFAIVLPSFIIMLTLSRFFF
                     VLQGNKYMEYALLGLRPAVIGLIAAAALVLMNSENFVDYKSIILFGIAFIASFKFKLH
                     PILLIILAGLAGLLLY"
     gene            complement(11890..12468)
                     /locus_tag="PKH68_01965"
     CDS             complement(11890..12468)
                     /locus_tag="PKH68_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNX11730.1"
                     /translation="MEKTPDVSSRIYWQMFITFVRIGAFTIGGGYAMIPLIQREVVQV
                     RKWMSPKEFIDMLALAQSAPGVIAINTAIFIGYKLKGVRGSIVTALGCALPSFVIILL
                     IAMVFTNFKDNPVVERIFKGIRPAVVALIAAPLYNMAKAAGVTWKTIFIPLIAALLIW
                     GLNISPVWVVVAAIAGGIIVGILRTRNLKKTE"
     gene            complement(12452..13399)
                     /gene="queG"
                     /locus_tag="PKH68_01970"
     CDS             complement(12452..13399)
                     /gene="queG"
                     /locus_tag="PKH68_01970"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNX11731.1"
                     /translation="MKATLTAYIKQTALEIGFDAIGVAQADFLREDAPFFKSWLDRGY
                     QAEMAYLERNQDKRLDPRELVEGCKSVIVVLMNYYPTVRQPEGAPLISKYSYSAVDYH
                     TVIKEKLLVLENRICDAFGTECFNYKQQHRFVDSAPVMERRWAERAGLGWIGKNKLLI
                     SPMFGSFCFIGILLINKELEYDERIPDRCGSCSRCMNACPANALSLTEGLNSNSCISY
                     QTIEKKGEISEEIRPALSGYVFGCDICQDVCPWNSKKSTPNKHEEFRPQDEILKWGKD
                     EWLSVQQEEFNRIFKTSALWRAGFQKLRENISYEYGKNS"
     gene            complement(13475..14605)
                     /locus_tag="PKH68_01975"
     CDS             complement(13475..14605)
                     /locus_tag="PKH68_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX11732.1"
                     /translation="MMRKLLFFSLLIFLTACNNPGKFTVAGTIAGADGKTLYFEKTGL
                     VSDSLLDSIKLNTNGNFTFKTAIPEYPDLYRLKLGNQLLVLAADSTTKKIEINGKSED
                     LLTAEIKGSVQSEEIQKLRKSVIQLQNEADQLRFAKNPEQSKQVLDTFIQHVNRHKKM
                     AIDLILKDTRSMSAYFAIYQQVSGTYLFSPFDKEDRQFYSAVATAFTTYMPDYERSKN
                     LYNLVIAAIQDERKGRLQQSWKELQQTSATGFIDIELNDAKGISHKLSSLTGKPVIID
                     FSVFESSSNIAYTFELRDIYNKYSPQGLQIYQISLDRNKMLWQKSVANLPWICVRDES
                     GKTATVYNVQSVPTLFLIDRQGNIVGRYEDIRQLAADISRAF"
     gene            complement(14614..15162)
                     /locus_tag="PKH68_01980"
     CDS             complement(14614..15162)
                     /locus_tag="PKH68_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNX11733.1"
                     /translation="MKRAFIFLANGFEELEAIAPIDILRRADIPVITVSVSDSKMVTG
                     AHNVIVVADQLFSETDFSDNAILILPGGGVGTKNLAAHKQLNELLLRQFEKNKNLAAI
                     CAAPSVFGNLGLLAGKEAVCYPGFEEKLVGADIPDQPVVKSDNIITARGAGVAVPFAL
                     KIVETLKGAELAHQIAKQIIFE"
     gene            complement(15203..15991)
                     /locus_tag="PKH68_01985"
     CDS             complement(15203..15991)
                     /locus_tag="PKH68_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNX11734.1"
                     /translation="MDKSKIYGLIGSAIFCLLTFLMLWFVLMPVPMMPQEDEGIMVSF
                     GDNPEGAGIGDGGSAKPTLENVANPVPVAVSAPKPAVSRPQEMMTSDDENALAIAAQK
                     KKDRERKRELDMQRLEQQRQAAAEAAEQKRRQEAIAKANDLMGGSFGNGGGSGSGTSS
                     GDTRQGNPAGSGTSGGHGWSLNGRSLVGSLATPSYPSNVEGRVTVAIRVDAAGHVTNA
                     SVSSPTNISDAQTRSAAVAAARNTRFSAGNGVSVGTITYNFKLK"
     gene            complement(15997..16422)
                     /locus_tag="PKH68_01990"
     CDS             complement(15997..16422)
                     /locus_tag="PKH68_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX11735.1"
                     /translation="MSLKRKYRTDPSFSMSSMTDIIFLLLLFFVISSTMTTPNDIKLN
                     LPSSKSEKATKKVIAKVSIDREGNYFVSRNNEKSVPVTPETLEPYILNLVAQDTTTYL
                     ALYADEEIAYKEVVRVLDIANQHKLKLVIATKAVGNTEN"
     gene            complement(16486..17187)
                     /locus_tag="PKH68_01995"
     CDS             complement(16486..17187)
                     /locus_tag="PKH68_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX11736.1"
                     /translation="MNLLVILQAVNAAVTEPALAQAAPERMNLWTMTLYGGPIMIVLA
                     VMLALGVYIFIERFITINKASQEDKTFMNRIKDYIHDGKLESAQKLCRDSETPASRMV
                     EKGISRLGRPMSDVLVAIENVGNIEIAKLEKSMGILSTIAGGAPMLGFLGTVTGMVQT
                     FYTMTQNPSGVVQLRDLSTGMYQAMVTTVGGLIVGILAYFAYNYLISKIDTVMRKLES
                     NTLEFLDLLNEPVKK"
     gene            complement(17263..17976)
                     /locus_tag="PKH68_02000"
     CDS             complement(17263..17976)
                     /locus_tag="PKH68_02000"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNX11737.1"
                     /translation="MTTLSVNINKIATLRNARGGNIPDVCKVALDCEKFGAEGITVHP
                     RPDERHIRYQDVLDLKPILTTEFNIEGNPTREFVELVKKVKPAQVTLVPDAHDALTSN
                     AGWDTKTHQDFLKEIVSGFQQAGIRVSIFVDTNPVNIEYAAKTGTDRVELYTEPYATN
                     YPNDKAAAVAPFVQAAKLAKSLGLGINAGHDLSLDNLAYLHQNIPWLEEVSIGHALIA
                     DALYLGLEETIKRYKAALK"
     gene            18225..19115
                     /locus_tag="PKH68_02005"
     CDS             18225..19115
                     /locus_tag="PKH68_02005"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNX11738.1"
                     /translation="MTEKETYRIAIFGNLHRTELGHQISQLIDFFRDKNIVILMDEFM
                     ENYIRETLKMSFENVELISGDNFTANLALSIGGDGTFLNTAARIGDKNIPILGINTGR
                     LGFLADIARNDVTEALKGIFENKFTIEKRSLLKVETLDGSRLDAPYALNDVALSKQDS
                     SSMIIVNAKTHGELINSYQADGLIISTPTGSTAYSMSVGGPIVVPQARSFVISPVASH
                     SLNVRPLIVPDDWVIELEVISRSNSYLISLDGRAKVMDQSTKLRIRKAEYDIKVLKPL
                     NHTFFETLRNKLLWGVDKRN"
     gene            complement(19112..19735)
                     /locus_tag="PKH68_02010"
     CDS             complement(19112..19735)
                     /locus_tag="PKH68_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11739.1"
                     /translation="MKVNNFIACVLLLVLLASCKTDTPNPPYVYENEPEFTWGYAQFY
                     GNYYSNYNLNNNVLTVNLFTDGLSVNENNELVGTGQYLILEDVFVAPLDSVLTAGTYT
                     ISAGGEPLTFFSGIKYEENNQEVPSGAYIYYIEQDVSKSKIVYIRDGTFTVSRQGISG
                     YSIDCRFVTNEKTEIKGRFNANLIFFDESAIPPAPNSAIRHRIKIRR"
     gene            complement(19801..21021)
                     /locus_tag="PKH68_02015"
     CDS             complement(19801..21021)
                     /locus_tag="PKH68_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX11740.1"
                     /translation="MKFPLWVAKRYLFSKKSHNTINVISGISAAGVAVGTMALVCVMS
                     VFNGFETLIEGMFSAFDPDLKITLVEGKTFNVNSPEFQKVRKLKSVANFTEIVEENAL
                     LRFRDKQMPATIKGVSPDFRAMTRIDSIMFDGEFVLTDSIITKAVTGVGVASELGMNA
                     TLFDPLMIYAPKRTERINILHPENSLNDVETYVSGIFIVNQAEYDNKYVFLSVETARR
                     LFEYDENTVSSVELKIKPTENPETVQNDIKKIVGERFRVLNRYEQQESFFKIMKIEKW
                     ITYLILCFILLIATFNIIGSLSMLIIDKKDDIQTLRSLGADNGLIKRIFLFEGWMISA
                     VGAVVGIVLGTALCLLQEHVGFLRLGEAYVVDAYPVATNGWDILLVLVTVLIMGVLAA
                     LYPVRYITTKKEID"
     gene            complement(21064..21414)
                     /gene="rbfA"
                     /locus_tag="PKH68_02020"
     CDS             complement(21064..21414)
                     /gene="rbfA"
                     /locus_tag="PKH68_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNX11741.1"
                     /translation="METTRQQKIARLLQKELGDIFLLYAKKMQGTLITVTAVRISPDL
                     SVARAHLSIFPADKVKSVLEQVNTDTKSIRYDLGKRVRHQLRIIPELYFHIDDSLDYL
                     ENIDNLLKQDKKEE"
BASE COUNT         5945 a         4644 c         4541 g         6326 t
ORIGIN      
        1 aattctggcc gaagctgcca aatcgcgcga catgatagta aacggagcaa aagataaagc
       61 tgcagaagaa gcggcaaaaa tcatggaagc agcccgcgaa cagatagcac ttgaaaaaga
      121 agatgccatc cgtcaaattc gtcgcgaagt ggcaaccctt tcggtagaaa tttcggaaaa
      181 agtgctccgt gaaaaactgg accggaaaaa ttcacaaatg gacatgattg accgtctgct
      241 ggacgaaatc aacattccaa aatcgtaaat tggcagaatt actgactatt aagggctaat
      301 tggtaatttg taacctgtaa caggcaataa aaatattagt ttaacttgta aatgtctatt
      361 cttgaacccc ctttcggggg gcaggggggc ttagtaatca aaaataataa tgaatacagg
      421 attaataccg gtacgatacg caaaagcttt gctggatttt gctcacgaaa caaaatcggt
      481 ggataaagtc tatggcgaag ccaaactcat tacgctcatg tttatgaaaa tgggcgaact
      541 gcgtcttgtg cttgaaaatc ctgttattga tcaggctgaa aagcggcaac tcatacttaa
      601 tgccgccggt ggtaaaacaa gcaagacatt tgagaatttt accgatttgc ttttgaagaa
      661 caacagggaa gcacatatac agaatatcat gctgaaattc gttgacctct accgggtgca
      721 aaaaaacatc cgttatggca aactcaccac agccactgcc gtggacgaat ctaccgaaga
      781 acggctgatg gcattgataa ctgataaagt gggcggtacc ctggaactgg aaaaagttat
      841 tgaaccggct attctgggcg gctttatcct cgaagtggat gacgttcgct gggatgccag
      901 catctccggg cagttgacca ggataaaaaa tgaatttatt gaaagaaaca gaagaatagt
      961 gtaaaaggaa gatgcaattg tcactacggg gaataattat attctaaaaa aacacgcagt
     1021 gataattttc aggtagaaaa aacaattgtc aggattaaga aaaaagaaat ttttatcaaa
     1081 aaaatatgtc agatagtata aaagtgagtg aagtatccga agtgcttcgg atgcaactcg
     1141 aaggaattga taccggcgtt aaatttgacg agattggaac cgtattgcag gtaagcgacg
     1201 gtgtagtacg tatctacgga ttgcgcaacg ccgaagccaa tgagctgctg gaattcgata
     1261 acggcatcaa ggctatcgta atgaacctgg aagaagacaa cgtaggggca gtattattag
     1321 gtccttcgag cgaaatcaag gaaggttaca gtgtaaaacg taccaaacgt gtggcttcca
     1381 tcttcgtggg cgaaggaatg cttggccgcg ttatcaatcc gctgggggaa gccctcgacg
     1441 gacgcggtaa ggtaaccggt gaactcaccg aactgcctct cgaacgcaaa gctccgggtg
     1501 taatctaccg tcaacctgtg actcaaccgc ttcaaaccgg tttgaaagcc attgacgcca
     1561 tgatacctat cggacgcggg caacgcgagc ttatcattgg cgaccgccag accggcaaaa
     1621 cgagtattgc tatcgatacg attatcaatc agcgtagaaa ttttgacgaa ggtgatcctg
     1681 tttactgtat ttatgtagct atcggacaaa aaggctccac tgtggccagt atagtgaata
     1741 cgcttaaaga acacaacgcc atggaatata ccattgttgt ggctgctaat gcttccgatc
     1801 cggctgcact ccaatactat gcaccgtttg cgggtgctgc catcggagaa tatttccgcg
     1861 ataccggccg ccatgcgctg gttatttatg acgacttatc gaaacaagct gtagcctacc
     1921 gcgaagtttc gcttattctt cgtcgtcctt ccgggcgtga agcttatccg ggtgatatat
     1981 tctacctgca ctcccgactg ctggaacgtg ccgccaaaat catcaatcag gaagaagtag
     2041 ccaaaaatat gaacgacctg cccgaaaccc tacgaagcag agtgaaaggg ggcggatcac
     2101 ttactgcatt accgattatt gaaacacaag ccggcgacgt ctcggcatac atcccgacaa
     2161 acgttatctc catcaccgac gggcaaatat tcctggatac cgacctgttc aatcagggaa
     2221 accgtccggc tatcaatgta ggtatctccg tatcgcgtgt gggtggtaca gctcagatca
     2281 aggctatgaa gaaagtggcc ggaacgctga aaattgacca ggcccagttc cgcgaactcg
     2341 aagcattcac caaattcggc ggcgatatgg accctgtaac agctatgacc attgacaagg
     2401 gacaaaaaaa cacccgattg ctggtgcagc cgctccatca acccatgccg gtggaaaaac
     2461 aggtagcagt gctgtactgc ggaactcacg gactgctatc caaagtaccg cttaacaaag
     2521 tcaatgattt tgaaaaggaa tttctcagta cgcttgaact gacacatcag gaagatgtac
     2581 tggatgttat caaaaaagga attattgatg atactgtcac ttccatactg gaaaaaacag
     2641 ctgaagaagt agcgagaaga atataatacc agtttgtagt ttgtagttta tagtttatat
     2701 tattgagttc ataaaattgc gagtcaaagt tctttgatct ttgttctttg ttctttaatc
     2761 tttgttctat aaatcatggc tctaacagtc taacagtccg accaatggct tcattaaaag
     2821 aaataaaatc aagaattcaa tcggtaaaat caacgcagaa aatcacttct gcgatgaaga
     2881 tggtgtcgtc ggctaaattg cgaaaagcac agaaaaggat tgagaatttt tatccctatg
     2941 aacggaagct gacccatatc ctgaataact ttctgagcgc cgaaaccgac aacacctctg
     3001 tatatgccga aaacagggag gttaaacggg ttgcaattct tgttttttcg tccaattcaa
     3061 gtctttgcgg cgggttcaat tccaatgtgg ccaaaagatt gcacgctgtg atggagcgct
     3121 acaatcacct ggggaaagaa aatatactga tttatccagt tggcaaaaag atagccaaag
     3181 tggcaaaaaa aatggatttt tttgtggata attttgatga aatggctgat aaacccacct
     3241 atgcagctgc tcaggatctg gccgacgatc tgatggagct ttttatcaat cacaccattg
     3301 ataaagtgga actgatttat catcacttca acaacaaaag ttcgcagata ctggtcgatg
     3361 aaacgttgct gcccattcgg ctggaagtca acccggaaaa acaaagcgga atgcaattga
     3421 attatattgt ggaacccgac cggaacacca taatgaatga cctgatacca aaagtgctgc
     3481 gtctgaaaac ctataccgcc ctgatcgact caaatgcctc ggaacatgca gcccgcacca
     3541 tagccatgca aaccgccacc gacaatgccg atgatttact tcaggaactc aacctgcaat
     3601 acaacaaatc gcgccagcaa gccatcacca acgaactgct ggatattatc ggaggttcat
     3661 tcggtcagaa ataaaaaaat tcttttattg gctttaaaaa attaattccg tccattacag
     3721 gaaaaaaaaa gcttttttca gctatttatt aaaaaaatcc aatcctttgt aaacgtgata
     3781 tcataattcg ggtaatcttt tttatactat tttttctacc tggcttgaaa aaaacttatc
     3841 cggatttgat tatttcattt acttcctgaa ttatttggat agcttttgat ttactaagac
     3901 cgactttttc agcaacaatt atcatatcat tctcagttgg ctcaccattg ttgtttattg
     3961 tagtagtatg aaatccgtta aaccctaatg atggcagcaa atcataagct ggtgacagtt
     4021 tccattcgtt attgatgagt tggaaagaaa aatttttcgc atgatcatca cggtttttaa
     4081 tggcaacatt gaacaccatc aaacgaaaaa gagcatacac ttcttccata tttttggtca
     4141 gaagaagaca caattttaat aaggattcgt aatccagact gggttcccga taattggcat
     4201 tgagtaatcc ggcagcactg atagtatgta ttttaccctg aggagttcgg tcgtatcgtt
     4261 ctacaccaaa ataccttccc tcaaaaagtc ttgtttccgg catttttatc ccgcattttt
     4321 tagctaacaa cgaatgctga tattctattc ttccgatatc aggtggatct ccgacagctt
     4381 tgaattttac gagccattct ttgtcatcca tgtttacaaa tacttttggt cgggcaccac
     4441 ccggcgagcc accgtagttg taaagagttt cgagcgaagt gtctgtgtat tttgttttaa
     4501 gaattttttc agtttctgca gccagaaatt cgagactgag aacatcaccg ggagttgatt
     4561 tgctatagtc cggtcggtat tccaaagctc cccgcccaca acttccgact aatgccaggc
     4621 gctgaagcaa agtaagtttg tccggattaa tatttttact cttcaggtag cgatccagta
     4681 tcagatttcc ccatccatcg ggcagactat catcaaatac accaaatccc ccgttgaaag
     4741 ggttacgttt agctataaat acttctttac gtagtggaag tattaatggt gaaatggact
     4801 ttcctgtatc taaatacgaa gaatcatact caaaggcaca caaagcatcg gaagttaatg
     4861 ctatccgacc taccttaaca gtgttcagaa acacttctat gacctttacc tcattcattt
     4921 ctcgatcctc tcagccgttt tttcacttct tgcgttttta gcaattcatc catattctga
     4981 tatgattttc gggaaaataa acctgcgaaa tcttctgcac tatccagggc aatggcacat
     5041 agaacaagat tttttaaggt aatttcaccc gaattttcaa acctgcgata ggcgtcatac
     5101 cccatccctg ccctttttga gaacgacttt tgtgtaagat taagttctaa tcttcgctcc
     5161 tttacccgac gggataaatt caggataatt gcatcgggnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnngt tctaatcttc gctcctttac ccgacgggat aaattcagga
     5341 taattgcatc gggcgtatta cttataattg tgtcgatatc catatttata acacataatt
     5401 gcacttaatt gtgctattat gcacaattac aaagatacat taaatatttc aatgttgatg
     5461 ttacaggtac attattatca accagaaatt gagatttttc taaattaagt ggtttataat
     5521 aattgtgtca atatagtgat ggataatcgg tgtttcagat aaaaaaaaga ggtttaaaga
     5581 aaaaatccgg aaccagtttg tgtaactgat taattttttg cttttatagt tcactcaaat
     5641 cgggcaagcc ttcgaacaat tcaagaatag aaaaatggta atttattacc acccctttat
     5701 gtacgccatc caccacatca ccctcacccg aagctctgcg aaatctgcgg gtgtcatttt
     5761 tatctttcag tcttattttt tttagttcat ccaggttttg agaaaaaaac ctcttgctcc
     5821 caggctccgt gcccaccatg cagggacgat tcaggaggca ccaggtgata aaaaccggga
     5881 caccctcacg ctctttgacc cggaaatacc tttcgagatc gcttaggtta agagcgagag
     5941 tttcagaagg atgtagtcct gaatcaggca ggtatttctg aacggacata aacgtacggg
     6001 attgaacttt gatttcgatg taaagacaaa cagaggagga atttatgcgg ctgacttcga
     6061 tatcaggaaa tccatcctga cgggttttta cagcaaaaag acccgggtat tttttgttga
     6121 ttattgctgt cacctcattt tcgacagctt ctgagaagtc cacatcatgt ttaaaatcga
     6181 actttggtac tgttacgccg tcgcatcggg tgcgacaggt tttgcagtcg taaatgaccg
     6241 gttttatttg ggaataattt gcagccatct gatataaaat ttctgacttt atattactga
     6301 tttgttgtat ttctcttcat attcatacct cagcgcatcg atggagataa gaactttatc
     6361 ccttttcaca taccagaagc tccaaccatt gttattcgac tttttaagga tatgagcact
     6421 cactttgtgg attgaaccca caaaaccatt gctcaccaat gttccatcgg cattaaccac
     6481 ggcacttatt gttccgtctt tggaatacag acactctcct accctgacgt aatctttcac
     6541 aataagacta ttgaatggaa ctttaggtat ccggttttcg atgggataac cggtcatctc
     6601 tttttgaact gtggtaattt tctccaaccg ttccctggcc acgctaatgt aaaattcatc
     6661 ccgttcgaag gcgatataat ttctgccgag aagtttagcc acggcagcgg tagttccact
     6721 tcccgaaaac ggatcgagca caatatcacc cggattggag gaggaaatca atacccgata
     6781 gagtagctct tcaggcttct gggttgaatg agccttcatt ccgttcacct ttatccgttc
     6841 gttaccctga caaatgggga ttacccagtc actacgcatt tgcaggtcat cgttcatcac
     6901 cttcatggag tgataatgga atgtgaattt cgatgatttg gattttgttg cccatatgag
     6961 tgtttcgtga gcattattga agcgtgttcc cttgaaattt ggcattggat tggttttcac
     7021 ccatataata tcgttcaaaa tccaaaaacc gatattctgc agcgcggttc ctaccctgaa
     7081 gatattatga tatgagccta taacccaaat acttccagtt ggtttgagca cccgatgaca
     7141 ctccgtaagc cactgatttg taaactgatc atattcagca aacgaatcga acttatccca
     7201 ttcatcatct acggcatcga cttttgacgt atcgggacga aaaagatcgc cattgagctg
     7261 aagattgtaa ggtggatcgg caaatatcaa atccaccgaa ttgtccggaa ttcgttttaa
     7321 taattccaca caatctccct gatgtatatt attgagttcg agagttccta aagttgtcat
     7381 atttcttaaa tcagttattt atcaatctta cgcaaatata cttatttttc gctcaaaaaa
     7441 gtcaataaaa aatctcctgc tgtataattc agtcaggaga ttaaatattt tgcttgaaga
     7501 ctctgtgtta tcttttaccg agcaatactt tgaatgtttc agtctggcct ttgttggatg
     7561 taacggcaag aacatatatc cccgaagcaa gtccttcgat catcagattt tcttttcctg
     7621 aactctgtcg gacagtactt ccgtttatgg atatcagatc aatttttgac acattgtcac
     7681 cggttatttg tatgacatct ttgcaaagtc tgaaagaata tctgtttaca attttttttt
     7741 ttaatcccgt attagcattt accggtacaa tctcaaacag atagctctcc tgtcgggttg
     7801 catttcctgc cgatatctta ttatccgtta ttgtccagaa ttttgttccc gcatcgcccg
     7861 cattctggat agcaaattta ttacccattt cggttactac ataagagttc cacgttgaat
     7921 aatatgaatt agttcctatt acgccaagct cattcacata ttttgtagtg tcggatgcat
     7981 aaaccagttt atatctgtcg gttgttttat ctaccagcag cttccattgt tgagttgccg
     8041 actggggtga tatctgtgct gaaaataccg gagtatttgt ggcacctttt gtcaggtatt
     8101 tatcgccaga tttgatgtaa taggtttcag caggaataat tatcgggtaa gtaatagtgt
     8161 cgttatctac cggtataatt tcaaaaataa agttgctgac cttaatctgg ttgatgctgc
     8221 taactccgat ccgggttgtg gttgaattcc agaatttatc acctcccgat ccgccattct
     8281 gaatggcgta tctgttgttc agtctgtata ggctgtaagt gttccaggct gcatcataag
     8341 cgttggtgcc aaagttaccc aactcattca catagcggga gtcctgggca tttacaattt
     8401 tgtagcgttc ggtaagcgga tcaatagtaa ttatccattc ctgacgttgc gggtttatat
     8461 tgtcgcgtgt gctcacatag gttggattgc cggttgtgcc aattttgttg gtcaggtaag
     8521 caccgttgta tttgatgaag tattttcctt cttccagaat tattgccggg aagatgtttt
     8581 tcatgtagtt aaagttacgt ttgtattctg taaccaacat ggctttcatc tgtctgatga
     8641 acggggccac tacttcgtta gcgggtttgg ggtttggcga tttgatggaa ccggtgaaat
     8701 ttcttgaaat aatctgtttc tgcgcatttt ccagtgagtc aacgtgcagg tattccctga
     8761 tgaaggcaac actatccttg tcgttaaggg ccttgtacat ttttaccaat gatactcctt
     8821 tggcgccaat aatatcgaat acctgcaacc aaggtttaat ttcagctatc atctccgggc
     8881 ttcctgtgct ggcaatcagt tctgctgagg catcacggaa actttggaac tgagcggcta
     8941 atgcatccgc tttgacgggg ctgtaagtgc ctgctttata agccgtcatg aatgggtcgg
     9001 ccacagcttt gaaaggagca gattctccct cgcgacgtaa tccgtgcgca gtggctccaa
     9061 ggtcgatgtt gttttcgcag aaaattttga aggccgcttc gttttgaggc atcaggtact
     9121 ttattcctct gagccatgag gcctgcggat tgtaggcggt catgttccag caatagtcag
     9181 cgatactgta caatgccact ttcgatgctt cggcatattc cattgggttg gcagtaaagc
     9241 caccgagttg ggcggcaatg ttggtgtcat ttccataagt gggacccatc aacaggtggt
     9301 caatgcaata atctgttacc gggtagttta gccagatata ggcttttctt ccaatctggg
     9361 cattaatcca gtccatatcg cttttgttaa tcatgtccac cacgctgttg ccggtccaca
     9421 tgatacgtac gcttttgtcc atttgcgatc ccagggtggt gaggtaagtg ccactcgacc
     9481 acgaacggtt gtattgggta gggcagaaag tgatggggcc tacatccgtt tttttgttga
     9541 cgaaattttg tactatgtaa ttggtggctg cacattgttg tacgtcggtt tcggtgccga
     9601 agtcatcgta gaagatggca aagtcgcgaa caccaatttt gtacatgtct tcaaatttgg
     9661 caataacttt ggcatattcg gttgtgttgg taaccccttt tccgcccagg tggatggccc
     9721 agataaattt cactttattt tcgtgtgcct ttcgaaccag ttcttttaac ttcagtgcct
     9781 gggcattggg gtaattaaca gcccattggc tgttgtggta gggatcatct ttgggtgaat
     9841 agatatagac gttcatcttg ttttgtccgt aaaactcaaa ctgacggata cggtcggctt
     9901 gtgaaaacgg atttccgtag aaaccttcca ctaaaccacg ttctatcacg ttagggaaat
     9961 ctttgatggt caccgacatc acttgtgggg cagctgcaat ttgaagataa gtttgaactc
    10021 cgtagtaggt tcctacgctg tcagttccgg caatggtcac ctttccgggt tcaactttca
    10081 ggtaatatcc ttctttaacg gcagggattt ctgaagcaac tgaagctaca ttagcatcgc
    10141 catttttacc gattaaaatt tctactccgg tagcagaaat gcttagtttt gattttagca
    10201 aagcgacagc gtccttatcg gcggttgctt caccgttgat gacgtaagtg gtggtgttgt
    10261 caaaggcttt gtttccccag gttatggatt ggggaattgg agatacggta acctgctgtg
    10321 cctgagctcc gaaaagagac atacaaagca atatcaggct gaatagtcgt gataatttca
    10381 tagcgataat ttatagatta gattttcatt taagtcagac aaaggtaagc ctttatttgc
    10441 taatttacaa aaatcaaaca gattcaaata aatcaagctg cctgtcatta tttttaaccg
    10501 gaaattcact cagataactg aatatctgcc gggtatcgga caggatgtga ttagtattac
    10561 atatttcaag aaaaagtttc atcagcgcag gattattctc gctcatgact tcataagcgt
    10621 ttccatattt atgctgatat ttttccttta ttcccggaaa atgctcatcc aatttgctat
    10681 aataatattc cctgtcaccc tcgcgtaggg tcactccaaa tccaaaacaa agtatcccct
    10741 tcacaccggc ttctatgcaa tagttgagaa taccggtcag attttcttcc gtatcattga
    10801 taaaaggcaa aatcgggcaa agccacacaa tagttggaat gccattatca cgcattattt
    10861 tcagcgtctc aaaccgttcc cgggtagtgc tcacattggg ctccacaata cggcacaaat
    10921 cctcatcaaa agtggtgagt gtcatctgta ccacacattt tgatttttga tttatttttt
    10981 tcaacacatc caggtcacga actatgcggt ttgacttggt gagaatgctc aatccaaatc
    11041 cgttttcctc aataatttcc aggcagcggc gggttaatcc cagattgctt tcaatgggca
    11101 gatacggatc gctcatggaa ccggttccta tcatacactt tttccgtttt cgtttcagtg
    11161 cctgctcgag caattcgggt gcattcgatt tgacttccac atcttcgaat gtatgattta
    11221 tctgatagca tttgctgcgg gaatcgcaat aaatacagcc gtgagtacat ccacgatata
    11281 aattcatacc gttttgggac gataatatgc tttttgcttc cttaaaatgc attttgttca
    11341 ttaaaaaaat gagggaaaat caatatagca gaaggcctgc cagaccggcc agaataatca
    11401 gcagaatggg atggagtttg aacttaaacg aagctataaa ggctatccca aagaggataa
    11461 tacttttgta atcgacaaag ttttcggaat tcatcagaac gagtgcagct gctgcaataa
    11521 ggcctatcac tgccggccta agtcccagca gggcatattc catgtattta tttccctgaa
    11581 gtacaaagaa aaacctggat aaggtcagca tgatgataaa agaggggagc acaatggcaa
    11641 aagtggcaat aacagagccg aggattgttc ccgaagccgt atagcccaca taggttgcgc
    11701 tgttgatgcc gataggtccg ggtgtcatct gcgaaatggc tatgatatcg gtaaactcgc
    11761 tttgcgtcat ccatccgtga atttctattt cgtgctggat aagtgaaatg atggcatatc
    11821 cgcctccaaa accgaataat ccgattttga aaaaggaaat aaaaagctgt atgtagattt
    11881 caatacccat tattcggttt tttttaagtt ccttgtccgc aggatgccaa cgattattcc
    11941 acctgcaata gcagcaacca ccacccatac gggtgatata ttcaggcccc agatcaacag
    12001 ggcggcaatc agtgggatga aaattgtttt ccaggttaca ccggccgctt tagccatatt
    12061 gtaaagcgga gcggcaatca gagcgactac ggctggccgg atgcctttga aaattcgctc
    12121 tacgaccgga ttgtccttaa aattagtgaa aaccatggcg attaaaagta ttataacgaa
    12181 tgacggcaat gcacaaccca gcgcagttac gatactcccg cgaacacctt tcaatttata
    12241 tccgataaaa atggctgtat tgatggcaat cacaccggga gccgactgtg ccagtgcaag
    12301 catgtcaata aattctttgg ggctcatcca tttcctgacc tgaaccactt cccgctgtat
    12361 gagcggtatc atggcatatc caccgccgat ggtgaaagct ccgatacgga caaatgtaat
    12421 gaacatctgc caatatatgc gggatgatac atcaggagtt ttttccatat tcatagctga
    12481 tattttctct taatttttga aaaccggctc tccaaagcgc tgaggtttta aaaatccggt
    12541 taaattcttc ctgttgcacg ctgagccatt cgtctttgcc ccatttcaat atttcatcct
    12601 gtggcctgaa ttcctcgtgc ttgtttggtg tcgatttttt gctgttccat gggcaaacat
    12661 cctgacagat atcgcatcca aaaacgtagc cggataatgc cggtctgatt tcttcggaaa
    12721 tttctccttt tttttcgatc gtctgatacg atatgcagct gttactattc aaaccttcgg
    12781 taaggctcag cgcattggca ggacaagcat tcatgcagcg actacaacta ccgcaacgat
    12841 cgggtatacg ctcatcatat tccagttctt tatttatcag caaaatacct ataaaacaga
    12901 atgatccgaa catcggactt atcagcagtt tatttttgcc aatccaaccc agacctgccc
    12961 gctcagccca tcgtcgctcc atgactggtg ccgagtccac aaaacggtgt tgctgtttat
    13021 aattgaaaca ttcggttcca aatgcatcgc aaattctatt ttccaacacc aacagttttt
    13081 cttttataac tgtatgataa tcaacagcac tgtatgagta ttttgaaatc agtggagctc
    13141 cttcgggctg tcgaactgtc gggtaataat tcatcagcac tacgatgacc gacttacacc
    13201 cttcgaccaa ctcacgcgga tccagtcttt tgtcctgatt acgttcaagg taggccatct
    13261 ctgcctgata acccctgtcg agccaggatt tgaaaaacgg agcatcctct ctcagaaagt
    13321 cagcctgagc tacgcctatg gcatcaaatc cgatttcaag tgccgtttgt ttaatgtaag
    13381 cagtcagggt tgctttcaat ttttctgttg tatttataga aataaaaaaa cacgatacct
    13441 gcttgctgca aaatatcgtg ctaaataatt tcgatcagaa tgctctgctt atatcggcgg
    13501 caagctgccg gatatcctcg tatctcccca caatatttcc ctgtctgtct atcaggaata
    13561 aagttggaac cgactgaaca ttgtacacag tggcagtttt accactttcg tcccttacgc
    13621 aaatccaggg caggttggcg accgattttt gccagagcat tttgttgcgg tcaagcgaaa
    13681 tctgatatat ttgcaatccc tgaggggaat atttgttgta aatatctctc aattcaaaag
    13741 tataagctat attacttgat gattcgaaga ccgaaaaatc aataataacg ggtttacccg
    13801 tgagtgatga tagcttgtgt gaaattcctt ttgcatcatt tagttcaata tcaataaatc
    13861 cggtagcaga ggtttgctgc agttccttcc aggattgctg caaccggcct tttctctcgt
    13921 cctgaatggc agcaattacc agattgtaaa gattttttga ccgttcgtag tccggcatat
    13981 acgtagtgaa agcagtagct accgccgaat agaactgccg gtcttcctta tcgaaaggtg
    14041 aaaaaagata ggtaccgctt acctgctgat aaatggcaaa atatgcagac atagagcgtg
    14101 tgtctttgag tatcaggtcg attgccattt ttttatggcg gttgacatgt tgaatgaatg
    14161 tatccaggac ttgtttcgat tgttcgggat ttttggcaaa ccgcagctgg tcagcttcat
    14221 tttgaagttg gatgaccgat ttgcggagtt tttggatttc ctctgactga actgagccct
    14281 ttatttcggc agtcaggagg tcttcggatt ttccgttgat ttcaattttt ttggtggttg
    14341 aatcggcagc caataccaac agttgattgc ccagtttaag gcgatacaag tcgggatatt
    14401 cgggaatggc tgttttaaaa gtgaaatttc cattggtatt cagcttgatg gaatccagca
    14461 ggctgtcgct taccaaaccg gttttttcaa aataaagcgt ttttccatct gcaccggcaa
    14521 tagttccggc gacagtaaat tttccgggat tgttacaggc agtcaggaag ataagcagcg
    14581 aaaaaaagag taattttctc atcatttggg tttttattca aaaataattt gtttggctat
    14641 ttggtgagcc agttcagcac ctttaagtgt ttctacaatt ttcagcgcga agggaacggc
    14701 cactcctgca cctctggcgg tgattatgtt atccgatttc accaccggtt gatccgggat
    14761 atcagcacct accagttttt cttcgaaacc gggatagcac accgcttctt tacctgccag
    14821 taaacccagg ttaccaaaaa ccgagggtgc agcacagatg gctgccaggt ttttattttt
    14881 ttcgaactga cgaagcaaca gttcatttaa ctgcttgtga gcagccagat tttttgttcc
    14941 cacaccgcca ccgggcaata tcaatatggc attatccgaa aaatctgttt cggagaatag
    15001 ctgatcggct acaacgatta cattatgagc gccggttacc attttactgt ccgatacaga
    15061 taccgttatc accggaatat cagcccgccg gagtatgtct atcggggcaa tagcttccag
    15121 ttcttcaaaa ccattggcca gaaaaataaa tgctcgtttc ataatccttc aattttaata
    15181 atgtgattat ttcagtttgt gattatttca gtttgaaatt ataggtaata gttccaaccg
    15241 acacaccgtt gccggccgag aatcgggtat tacgggcagc agctacggct gcactgcgtg
    15301 tttgtgcatc ggatatattg gtaggagaac tcacacttgc attagttaca tgacctgcag
    15361 catccacccg gatggctaca gtcactcttc cttccacatt gctgggatag gatggtgtgg
    15421 caagtgagcc taccagtgag cgtccgttca gagaccatcc atgccctccc gaagtaccgc
    15481 tgccggcggg attgccctga cgggtgtcgc ctgaagaggt tccactaccc gatccgcctc
    15541 cattgccgaa agaacctccc atgaggtcat tcgctttggc gatagcttcc tgcctgcgct
    15601 tctgttcggc cgcttcggcg gctgcctgcc gttgctgttc gagccgctgc atgtcgagtt
    15661 ctcgctttct ttctctgtcc tttttctttt gggccgctat tgccagagca ttttcatcat
    15721 cgctggtcat catttcctgt ggacgggaaa cagccggttt gggcgctgaa accgccacgg
    15781 gtacaggatt agccacattt tccagtgttg gcttggctga gccgccatca ccaattcccg
    15841 caccttcggg gttgtcgcca aaactgacca tgatcccttc gtcttcctga ggcatcatgg
    15901 gtacgggcat gagcacaaac cacagcatca ggaatgtaag caggcaaaaa atggccgaac
    15961 cgattaatcc gtatattttt gatttatcca aaattatcaa ttttcagtat ttccaacagc
    16021 tttggtagcg attacaagtt tcagtttgtg ctgatttgca atatcgagta cacgtaccac
    16081 ttctttgtag gctatttctt catcggcata gagtgccaga taggttgtgg tgtcctgcgc
    16141 taccagattg agtatgtagg gttccagcgt ctcgggagtt accggaaccg atttttcgtt
    16201 gtttcgcgaa acaaaataat ttccttccct gtcaatggat actttcgcaa tgactttctt
    16261 ggttgctttc tccgatttgc tcgatggcaa attgagctta atatcattgg gagtggtcat
    16321 tgtggatgat atcacaaaaa acagcagcaa caaaaaaatg atgtcggtca tggacgacat
    16381 tgaaaaggat ggatctgttc tgtattttct ttttaatgac attgtatttt aagtttattc
    16441 attcaattag aaatattgaa ttgataataa taaccaaact atgaattatt ttttaaccgg
    16501 ttcattgagt aaatcaagaa actccagcgt gtttgattcc agtttacgca ttacagtgtc
    16561 aatttttgag atcagatagt tgtaggcaaa ataggccaga atacccacaa tcaacccgcc
    16621 cacagtggtg accatagcct gatacatacc ggtggataaa tcgcggagct gcactacgcc
    16681 cgagggattt tgggtcatgg tatagaaagt ttgcaccata ccggtaaccg taccgagaaa
    16741 accgagcatg ggtgcaccgc cggcaatagt tgagagaatt cccatgcttt tttccagttt
    16801 ggctatttcg atgttaccca cattctctat agctacaagc acatcgctca tggggcgacc
    16861 caaccggcta atgccttttt cgaccatgcg tgatgccggg gtctccgagt cgcggcaaag
    16921 tttttgtgcc gattccaatt ttccatcgtg aatgtagtcc tttatcctgt tcataaaggt
    16981 tttatcttcc tgcgatgctt tattgatggt tataaatcgt tcgataaaaa tatacacccc
    17041 gagtgcgagc atcaccgcca gcacaatcat aattggacca ccgtaaagtg tcatagtcca
    17101 taaattcatt ctttcgggtg cagcctgtgc gagagcgggt tcagttactg cagcatttac
    17161 tgcctgcaaa attacgagta ggttcatctt ggattatttc tttttttctg attaatatta
    17221 ttctgacaaa aaaggctgaa tatctgcggt tttttatcaa ccttatttta acgctgcttt
    17281 atagcgtttt attgtttctt ccaatcctaa atagagggcg tcggctatga gtgcatgccc
    17341 gatagacact tcttcgagcc atgggatgtt ttggtgaaga taggccagat tatccaaact
    17401 cagatcatgg ccagcattga tacccaaacc aagacttttg gccagtttgg cagcctgtac
    17461 aaaaggagct actgctgcag ctttatcgtt gggataattg gtggcataag gttcggtata
    17521 aagttccacg cggtctgttc ctgttttagc agcatattca atgtttaccg gatttgtatc
    17581 cacaaatatg gagacccgaa tgcctgcttg ttgaaaaccg gaaacgattt ctttcaggaa
    17641 atcctgatga gttttggtat cccatccggc attggaagtg agagcatcgt gtgcatcggg
    17701 cacaagcgtt acctgagcag gcttcacttt ttttaccagt tcaacaaatt cgcgggtagg
    17761 atttccttct atattgaatt ctgtggttaa aatcggcttc agatccaaca catcctgata
    17821 ccggatatgt cgttcgtcgg gacgcggatg aaccgtaatg ccttcggcac cgaatttttc
    17881 gcagtcgagt gctactttgc acacatcggg gatattgccg ccgcgtgcat tacgcaacgt
    17941 agctatttta ttgatattta cacttaaagt ggtcattatt ccggtaattt taattaaata
    18001 aaaagactta tattttcaga gaatttagtg agtcattttc taacccgaag attctgaaat
    18061 gaaattaaat ttgtgagttt caaatccatt ttgttccttt gcaggtcaaa tgatttagta
    18121 tttgcaaaaa taactcaaaa aaacgagtaa acgaataaag aatttatact ttcgtatttt
    18181 ttaatgattt tgacacggtt acaatatcga tatcaaggca tcctatgaca gaaaaagaaa
    18241 cttacagaat agccattttc ggaaatctcc accgtacgga actcggccat cagatttcgc
    18301 agttgatcga tttttttcgt gataagaata tagtgattct gatggatgaa tttatggaaa
    18361 attacatccg cgaaacgctg aaaatgtcat ttgaaaacgt ggaacttata tcgggggata
    18421 atttcacagc caatctggct ctcagcattg gtggtgacgg cacatttctg aacaccgccg
    18481 cccgcatcgg cgataaaaat atacctattc tgggaattaa caccggaagg ctgggttttc
    18541 tggctgatat tgccagaaac gatgttaccg aggcgttgaa aggaattttc gagaataaat
    18601 ttaccatcga aaaaagaagc ctgctgaagg ttgaaaccct cgacggaagt cgcttggatg
    18661 caccttatgc gttaaacgac gtggctctgt cgaaacagga cagttcatcc atgatcattg
    18721 taaatgccaa aacccacggt gaactgatta acagctatca ggctgatggt ctgatcattt
    18781 ccactccgac aggttcgacg gcctactcca tgagtgtggg cggccctatt gtagttccgc
    18841 aggcaaggag ctttgtgatt tcgccggtgg catctcacag cctgaatgta cgaccgctta
    18901 ttgtgcccga cgactgggta atcgaactgg aagttatcag ccgcagcaac agctacctga
    18961 tatcactcga cggccgggcc aaggtaatgg atcagagcac taaacttcgc atacgaaaag
    19021 cggaatatga tatcaaggta ctgaagccat taaatcacac tttttttgaa accctgcgga
    19081 ataaactgct ttggggtgtg gacaaaagaa attagcgacg gatttttatc cggtgacgga
    19141 tagccgaatt gggagcgggc ggaatggccg attcatcgaa aaaaatcaaa tttgcattga
    19201 accttccttt tatttcggtt ttttcattgg tcacaaaccg gcaatcgatg ctataccccg
    19261 aaattccctg cctgctgaca gtaaaagttc catccctgat ataaacgatt tttgatttgg
    19321 acacatcctg ttctatgtaa taaatataag ctccggatgg cacttcctgg ttgttttctt
    19381 catattttat accactgaaa aaagtcaacg gttcgccgcc ggcagaaatg gtgtaagtcc
    19441 cggcagtcag cacactatcc agtggcgcca caaaaacatc ttccagaatc aaatactgac
    19501 cggtaccgac cagttcatta ttttcgttga ccgacaaacc gtcggtaaag agatttacag
    19561 tcaacacatt gttgttcaga ttgtaattgc tgtagtaatt gccgtaaaac tgtgcatatc
    19621 cccacgtaaa ttcaggttca ttttcatata cgtatggcgg attgggggtg tcggtcttgc
    19681 aggaagccag cagcaccaga agtaaaacac aggctatgaa attgttgact ttcatgtgat
    19741 tatcatctaa taaatattcg ttattattaa atcaaaaccc gtaagctgtt gatttattgt
    19801 ctaatcaatt tcctttttgg tagtaatgta ccggacggga tagagcgcag ccaacacccc
    19861 cataatgagc acggtaacga gtacgagcag aatatcccaa ccgtttgttg ccaccggata
    19921 agcatccacc acgtatgctt cacccaatct gagaaaccca acatgttcct gaagcagaca
    19981 aagagcagtt ccaagcacaa ttcccaccac agctccaaca gccgaaatca tccatccttc
    20041 gaagagaaaa attcgtttaa tcagcccgtt atcggctcca agactgcgga gtgtctgaat
    20101 atcgtccttt ttatcgatta taagcatcga aagcgagcca atgatgttga aagtggctat
    20161 cagcaggatg aaacagagaa taagatacgt aatccatttc tctattttca tgattttgaa
    20221 gaaactttcc tgctgttcgt agcggttaag aaccctgaat ctctcaccca caatcttttt
    20281 tatgtcattc tgaaccgttt cgggattttc ggtgggttta atcttcaact ccactgaaga
    20341 aacagtattt tcgtcatatt caaagagtcg gcgggcagtc tccaccgaaa gaaaaacata
    20401 tttgttgtcg tattcggcct gattaacgat aaatataccg gacacatagg tttccacatc
    20461 attcagactg ttttccggat gaagaatatt tatccgttcg gtacgtttgg gcgcataaat
    20521 catgagcgga tcgaacaagg tggcattcat accaagttcg gatgctacac caacaccggt
    20581 cactgctttg gttataatac tgtcagtcag tacaaattca ccatcaaaca tgatgctgtc
    20641 gatgcgggtc atcgccctga aatcggggct cacccctttg atggtagccg gcatttgttt
    20701 atcccggaaa cgcaggagtg cattttcttc aactatttcc gtgaagttgg cgactgattt
    20761 gagtttacga accttctgaa attccggact gtttacattg aacgtttttc cttcaaccag
    20821 ggtgattttc agatcgggat cgaaagccga aaacatccct tcgatcagtg tttcgaagcc
    20881 attgaaaaca gacatcacac agacgagcgc catggtgccg accgccacac cggcagccga
    20941 aattcctgaa atgacgttga tggtattgtg cgatttcttt gaaaaaagat atcgcttagc
    21001 cacccacagc gggaacttca atgaagctga tgaatgtgat ttgttttcgg ccattctctg
    21061 aaattattct tctttcttgt cctgtttcag caggttatca atgttttcca gataatccag
    21121 gctatcgtcg atgtgaaaat aaagttcggg gataatgcgc aattgatggc gaaccctttt
    21181 tcctaaatcg taacgaatgg atttggtatc ggtattcacc tgttccagta ctgatttcac
    21241 tttatcggcc gggaaaatgc tgagatgtgc acgtgccaca ctcagatcgg gactaatacg
    21301 tactgcggta acggtgataa gtgttccctg catttttttg gcataaagta aaaatatatc
    21361 acccaattcc ttttgaagca gtcgcgctat tttttgttgt cttgttgttt ccataattta
    21421 gttttttata ttttccactc aaatccatct ttggtatcct ttatttcaaa tcccaaagca
    21481 gtcagttcat tacggatctt gtcgctggtt gcccagtctt tgtttttctt ggcttcgagc
    21541 cggatattta aaagca
//
LOCUS       DAOPZK010000017        21287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2536_length_21287_cov_22.363649, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000017 DAOPZK010000000
VERSION     DAOPZK010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21287
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2536_length_21287_cov_22.363649"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..654
                     /locus_tag="PKH68_02025"
     CDS             112..654
                     /locus_tag="PKH68_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11742.1"
                     /translation="MNLEELKRDCAIKQKKGLHFILGSIAIWGLMAIIHMTDLPILTK
                     NMLTFCATAPLVPIAYLISRLIKVEFSDKKNPLNQLGLLFSLNQMIYLLIAMWVYSAV
                     PDKMVMVIAMIFGAHLLPYAWLYSSRIYGIMSIIVPISALIVGINFEAYILAILMLIY
                     EVVFSLVLAMEVKKIDRVKA"
     gene            836..1402
                     /locus_tag="PKH68_02030"
     CDS             836..1402
                     /locus_tag="PKH68_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNX11743.1"
                     /translation="MTTDKILNIKSRAEWRNWLQQNFNIEKEVWLVNAKKSSGELRIE
                     YNDAVEEALCFGWIDSTNKTLDKDHTIQRYTPRQTRSSYSQPNKERLKRLAEKNLIHH
                     SILKTVHEIISEEFIFPSDIMDEIRKNKTAWNNYLKFPEPYKRIRIAYIDSARKRPEE
                     FTKRLTNFILKTQNNKLISGFGGIDKYY"
     gene            complement(1503..2441)
                     /locus_tag="PKH68_02035"
     CDS             complement(1503..2441)
                     /locus_tag="PKH68_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11744.1"
                     /translation="MKNKIYLPVIFGLVTLIAACNPTPPIDDTMLDSGKYSFAVPDSL
                     QKRLVSNYIANPTEVQKNTPVIIAAHGYSATTFEWDELREYADSARTFYVSQVLLGGH
                     GRSYTDFKSSTWQVWQSSIRDEYVKLSQKGYRKIYLAGSSTGGPLIIQLLTTTLFTED
                     PGLEGVFLIDPIVVSSDKQLSLVGLLGPVLGYTETEMSDGEKGKWYVYRPQETLRQLM
                     SLIDLTRRELQRGIKVPDEIYMKVYKTVKDDAADPVSAVLIYNGIKYDNKHIDVEMID
                     SRLHVFTRLRGRAEITEKDRSLQRKAFREMEAKMTK"
     gene            complement(2487..3035)
                     /locus_tag="PKH68_02040"
     CDS             complement(2487..3035)
                     /locus_tag="PKH68_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11745.1"
                     /translation="MKRIGYLLIFVLGTFSTMNAQQAVSELNVGLLVQKTQHLYWENG
                     IGVDYASDVLFNRHIHLKAGYISSRFGSSLVSNAVKQDNLLLGVDFRFRPTKPFQIFA
                     GVNTGYFYADYENPAFDVLPNSSLLFSAETGVVYRFKAPHVTLGLSAGYNFINGDGIS
                     KPGTLFPVFYKMSLFYNIGTSK"
     gene            complement(3102..5006)
                     /locus_tag="PKH68_02045"
     CDS             complement(3102..5006)
                     /locus_tag="PKH68_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX11746.1"
                     /translation="MKPTIIDMFNSSVEKFAQNPFLWEKDTNEFVPTTYLETQQQAVL
                     FAAGLLAAGVKPGDKTALLSEGRNAWIISELGILHAGAINVPLSIKLEESNDLVFRII
                     HSESKFIVVSGGQLPKIRKIIASLHDVEKVIVLDEQTDYQPKEIPLSELLAAGKVLLD
                     KTPEIVTERYKTIRPDDIATISYTSGTTADPKGVMLTHRNYTANVEQALTLMTIPSTF
                     RTLIILPLDHCFAHVAGFYSFMASGASVATVKSGKTGIETLKNIPINIREIRPNLLLS
                     VPTLTKSFKRNIESSIHAKGPVVTWLFNTAIKMSYAYNKEGFNKGQGIQAIYKPLLML
                     FDKILFSKVREGFGGQIEYFIGGGALLDIDLQRFFYAVGMPVFQGYGLSEATPIISSN
                     GLKRHKLGSSGYLVSPLELKICDDNDNALPNYQKGEIVIRGENVMKGYYKNEKATAET
                     LRDGWLHTGDLGYMDDDGFLYVMGRFKSLLISSDGEKYSPEGIEESMVENSYFIDQVV
                     LHNNQHPFTSAIIAPNKEALKRYVHRKKPELSWETREAKELALTKLQREVNEYRKGGR
                     FEGMFPERWLPSAIAVIGEPFTEQNGLVNSTMKVIRGKVEERYADRIADMYEAGGKDI
                     INEKNMEALT"
     gene            complement(5352..6167)
                     /locus_tag="PKH68_02050"
     CDS             complement(5352..6167)
                     /locus_tag="PKH68_02050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX11747.1"
                     /translation="MMNRIYSFLLTILIGVLFTACSNSTQKDKSQPAENKSSEVNMNS
                     AAEIMAKPQVPVLCYHRITTGRNDEYTVTPAAFGAQMQLLKDSGYHSINPDELYDYLV
                     YNKPLPEKPFMITFDDSRVEHAAIAAPEMEKRGFHGVFFIMTITYNKKNYMTTDQIAG
                     LAKNGHTVGLHSWDHTMATKYLTDSVAVQQIVKPREKLEGITGKKVEYFAYPYGITNH
                     ETSVAMEKYFKLSFILSTKRDSIAPLQTVRRMIVPSGWTPGGMLKAMHKTFGS"
     gene            complement(6244..>7281)
                     /locus_tag="PKH68_02055"
     CDS             complement(6244..>7281)
                     /locus_tag="PKH68_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX11748.1"
                     /translation="RLAVRYSKLKIFRILKLVEIPIMLLACVAFYYQWVIVAVLSVFA
                     LGTQSCLYSPSKYSLIRDIGGEEGASYGSGMFETMAFMGILLGTFVASVVSDHYSPGL
                     FGLIALGVAVLGYYSSKTIRAVELPEEQSQSSIVNPILFVMANYRYARNYRLVNSAVF
                     GSSAFWLIANMVQMNIIIHCKGHYQTSNSMTGVVMACAAVGIAIGTGITGNVMGEKVN
                     KGLILPALAGMIAALLVVVFIPLSFPAFVGMVVVFAFMAGMFQVPCMAMMQQADLGRK
                     RGDTVAYLNMMNFVFILLGTAVFSLTTALTDENSFTVFGVMIFLCLLIAAYFLFRYPD
                     FRKETATSLKK"
     assembly_gap    7282..7381
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7594..9111
                     /locus_tag="PKH68_02060"
     CDS             7594..9111
                     /locus_tag="PKH68_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX11749.1"
                     /translation="MKKSNIWILIILMAITFGALILLQARFLKATSEMAEKQFDDAVK
                     RSLIQTANILEENEALEYLAQTLDENDYIQTENKTGNFGVNKQKDTLNTNWKSTSAVS
                     PRIRLSTGHGKASIEQTSKYLQQRFQLNFSRSKTVLDQAVFRWLRENENKEISERVDY
                     TDLNETLETYLKSNNVDLPFYYTIADQRNKVIYQCNKTINTKGKPNTDIYVQELFPHE
                     DSSRKAYMQVVFPTKQSFIYESLNLLLPSIGVILLTFVIFVLTIFIIFRQKQLNNMKN
                     DFVNNMTHEFKTPISTISLASQMLQDPGVAKNPDMMKHVSNVIRDETKRLSLQVEKVL
                     QMSMFEKEKSTLKFTEIHINNLIKDIIGSFSLKVTSKGGKINSNLKAKQDMALVDEIH
                     FTNVIFNLMDNALKYSDKPLILNLETWNEKDNLLISVEDNGIGMKKEDLKRIFEKFYR
                     VSTGNLHNVKGFGLGLAYVKKMVNDHNGIIKVESEPGLGTKFTIIIPTLKKNNYE"
     gene            9104..9811
                     /locus_tag="PKH68_02065"
     CDS             9104..9811
                     /locus_tag="PKH68_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX11750.1"
                     /translation="MNDEKVRILLCEDDENLGMLLREYLQTKGYYCDLQPDGEAGYKS
                     FSRNKFDLCVLDVMMPKKDGFALAADIRAINSEVPIIFLTAKSMKEDILQGFKLGADD
                     YLSKPFSMEELLYRIESILRRVRGKKSKDVVTYQIGGFTFDAQKQTLIFEGEIKKLTT
                     KESELLNLLAANANNILERNFALKTIWVDDNYFNARSMDVYITKLRKLLKDDQNVAII
                     NIHGKGYKLIMPQVKED"
     gene            complement(9866..>11698)
                     /gene="mutL"
                     /locus_tag="PKH68_02070"
     CDS             complement(9866..>11698)
                     /gene="mutL"
                     /locus_tag="PKH68_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNX11751.1"
                     /translation="IHLLPDSVANQIAAGEVIQRPASVIKELVENSIDAGAKNIQVLI
                     KDGGSTLIQVIDDGVGMSETDARMSFERHATSKIKDAADLFSLRTMGFRGEALASIAA
                     VAQVELRTRRENEELGTLIEIAGTRVFRQETVQCDKGTNIAVKSLFFNVPARRRFLKS
                     PSTEMTHIRNEFYRIVLVHPDISFFLYDNDTEMMTLPATSLKARIEHVFIGAYRKKMD
                     QQLLPIEAKTSLLTITGFVGRPEFAQKIANQYFFVNGRYMRHPYFHKAVMVAYSQLIK
                     PADNPNYFIYFDIDTQSIDVNVHPSKTEIKFENEQAIWSILLASVKESLGKFQVMPPL
                     DFDQDGLPDIPVIDSSTEIQVPKTNFDPGYNPFGATTRPKRPTLDWEQLYGDFSKPNR
                     QPGFQNAPTDSEAGFPAPLQPEQQQELDIPDTSTDFFQFKNKYILTTVKSGLMLIDQH
                     RAHFRILFDRFIQKSGPQKGITQQVLFPEILEVERDDLHIVEQILPDLRWVGFDIEPI
                     GKSEFAINGIPSEIEMTSTLPLLKDLIAAVRETEISVKETIREKIAVHLSRQTAIQYG
                     QSLTGAEMKELFESLFACENHQYTPDGQMIVTMMKYEEIENKFK"
     assembly_gap    11699..11798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11799..12841)
                     /locus_tag="PKH68_02075"
     CDS             complement(<11799..12841)
                     /locus_tag="PKH68_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNX11752.1"
                     /translation="MENNYCVIMAGGLGSRFWPFSRNDKPKQFLDFFGTGRSLIQMTY
                     DRFRAIVPAANILIVSNVIYRELILQQLPEIKPEQVLLEPARRNTAPCIAYAIQRIKA
                     ITDKANIIVAPSDHLITKEAEFLETIKTGLDFVDKNDSLLTLGIKPSRPETGYGYIQV
                     DEGSTNPRKVKTFTEKPNAELAQIFFETGEFFWNSGIFLWNLQAIDNAFRTYLPDVAA
                     KFNEGKNYYNTDKEQDYINEMYPSCPNISIDYGIMEKAERVHVLCADFGWSDLGTWGS
                     LYDQSAKDEKNNVTLKCEAIFYESEDNIVALPSGKLAVIHDLNGYIVAESDNVLLVCK
                     KSDEQRIRQFVNDS"
     gene            complement(13135..17433)
                     /gene="rpoC"
                     /locus_tag="PKH68_02080"
     CDS             complement(13135..17433)
                     /gene="rpoC"
                     /locus_tag="PKH68_02080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNX11753.1"
                     /translation="MAFKNDNKTKSSFNKISIGLASPEEILRLSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPTKDFECHCGKYKRIRYRGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWFFRSLPNKIGYLLGMPTKKLDAIIYYEKYVIIQAGILDNGENITNNELL
                     TEEEYLNLLDSLPRDNQLLEDTDPNKFIAKMGAEAIYDLLTRLDLDTLSYDLRNKANT
                     DTSQQRKTEALKRLQIVESFRASKTRNKAEWMIMKIVPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSSAVKTDAN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKP
                     FVIRKLIERGIVKTVKSAKKIIDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAF
                     QPKMIEGKAIQLHPLACTAFNADFDGDQMAVHLPLGNEAVLEAQMLMLASHNILNPSN
                     GAPITVPSQDMVLGLYYITKPRPGSKGEGLIFYSPEEAEIAYNEGVVLLHSLIKVRTK
                     DLDENGELTVRLIETTVGRILFNKHVPEEMGFINELLSKKSLRDIIGRVIDTCGVART
                     ALFLDDIKDLGYYMAFRGGLSFNLSDVIIPKEKETLVQEGNAEVEEIMNNYNLGFITP
                     RERYNQIIDTWTHVNTKLSNVLIKQLAEDRQGFNSMYMMLDSGARGSKEQIRQLAGMR
                     GLMAKPQKSGAEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVSQDVIINEEDCGTLRGLVATEIKNNEDVISSLYERILGRVSVHDIYHPLTG
                     ELLVTSGEQIVEVAARKIQDSPIERVEIRSVLTCESKKGVCAKCYGRNLATGKMVCQG
                     EAVGVIAAQSIGEPGTQLTLRTFHVGGIASNIAAESKIILRYDGRLEFDELRTVDAVD
                     EEGKPCRVVVSRLTELRVVDPNTGIILTTQNVPYGSTLYAKHGDLGKKGQFICSWDPF
                     NAVIIAETEGKIEFEDVIENVTYKTEVDDATGLREKIIIEAKDKTKVPTANVVDVEGN
                     IRNYNLPVGAHLVLDDGSTIKAGQMLVKIPRAIGKAGDITGGLPRVTELFEARNPSNP
                     SVVAEIDGEINFGKIKRGNREVSVTSKTGEVKKYLIPLSKQILVQENDFVRAGTPLSD
                     GAITPADILAIKGPTAVQDYIVNEVQDVYRLQGVKINDKHFEVIVRQMMRKVLIEDQG
                     DTRFLEKQVVDKHEFMEENDRIWGKKVILDPGDSESLKAGQIITARKLRDENSVLKRK
                     DLKLVLTRDAVAATSSQVLQGITRAALQTNSFMSAASFQETTKVLNDAAINGKIDKLE
                     GLKENVICGHLIPAGTGLREYEKIIVGSRDEYEKMMDARKNVLDISQDEDVETE"
     gene            complement(17554..>21287)
                     /gene="rpoB"
                     /locus_tag="PKH68_02085"
     CDS             complement(17554..>21287)
                     /gene="rpoB"
                     /locus_tag="PKH68_02085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNX11754.1"
                     /translation="QLKSFQDFFQMDTAPEKRKTEGLYKVFNENFPIADTRNNFVLEF
                     LDYFIDPPRYSIEECFERGLTYSVPLKAKLKLYCTDPEHEDFDTVIQDVYLGPIPYMT
                     PKGTFVINGAERVIVSQLHRSPGVFFGQSMHANGTKLYSARIIPFRGSWIEFATDINN
                     VMYAYIDRKKKLPVTTLLRAIGFESDKDILEIFNLAEEVKVNKTNLKKVLGKKLAARV
                     LHTWNEDFVDEDTGEVVTIERNKVIIDRETILENSHIEEIIESGTQTVLIHKDDANQT
                     DYAIIYNTLQKDPSNSEKEAVLYIYRQLRNAEPADDSSAREVINNLFFSEKRYDLGDV
                     GRFRINRKLNLSTNSDIKVLTKEDIIEIIKYLVELINSKAEVDDIDHLSNRRVRTVGE
                     QLHNQFGVGLARMARTIRERMNVRDNEVFTPIDLINAKSISSVINSFFGTNALSQFMD
                     QQNPLAEITHKRRLSALGPGGLSRERAGFEVRDVHYTHYGRLCPIETPEGPNIGLISS
                     LCIYAKINELGFISTPYRTVTNSIVDLSPEGIKYYTAEEEEDLVVAQGNAPLNDDGSF
                     IRKVVKTRLEADFPVVPPSDVNLMDVAPSQIASIAAALIPFLEHDDANRALMGSNMMR
                     QAVPLLRCDAPIVGTGIEGQLIRDSRTQITAEGEGEVEFVDANTIKVRYNRSEEEEFV
                     SFDTAVKTYELPKFLKTNQNTTIDLRPIVTKGQKVTAGQIMTEGYSTHNGELALGKNL
                     KVAFMPWKGYNFEDAIVINERVVREDIFTSIHVVEQLLEVRETKRGIEEFTSDIPNVS
                     EEATKDLDENGIIRIGARIEPGDIIIGKITPKGESDPSPEEKLLRAIFGDKAGDVKDA
                     SLKATPSLSGVVINKRLFSKAQKNRKSKLADKAVLPKLDEEFEAQSAALKERLIEKLL
                     IIIEDKTSAGVKDYLGADIITRGSKFTAERLREVDYTTVQLGKWTTDSHKNDLIRLLI
                     LNYLHKYKELDAQIKRQKFNITIGDELPNGIVQLAKVYIAKKRKIRVGDKMAGRHGNK
                     GIVSKIVRQEDMPFLEDGTPVDIVLNPLGVPSRMNLGQIFETVLGWAGKELGVRFATP
                     IFDGASLDDLDQWCDKAGVPRYGKTYLYDGGTGERFDQTATVGIIYMLKLGHMVEDKM
                     HARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAFGASHILQEILTVKSDDVMGR
                     ARTYEAIVKGDPMPTPGIPESLNVLLHELRGLGLSINLE"
BASE COUNT         5998 a         4595 c         4313 g         6181 t
ORIGIN      
        1 aagggttgta gcccgcttca atttttcatg taacctgaca gggaatcgca atcccttact
       61 gaccataccg ccgtccgtta tacattacaa aagagctaag gagaatttac aatgaattta
      121 gaagaattaa agagagactg tgcaataaaa caaaagaaag gtttgcactt tattttaggg
      181 tcaatcgcta tttggggatt gatggcaatt atacatatga ctgatttgcc aatactgact
      241 aagaatatgt taacattttg tgcgacagca cctttagtac caattgctta tctgatatca
      301 aggctaataa aggttgagtt tagtgacaaa aaaaatccgt taaatcagtt gggtttgctt
      361 ttttcgctaa accagatgat atatctactg atagcaatgt gggtatattc tgcagtgccc
      421 gataagatgg taatggtaat tgcaatgata ttcggggcac atctgttacc ttatgcatgg
      481 ttgtatagct ccagaatata tggtataatg tcaataatag ttccgataag cgctcttata
      541 gtgggaataa attttgaggc atatatttta gccattttaa tgttaatata tgaggttgtt
      601 ttcagtttgg tacttgctat ggaagtcaaa aagatcgaca gagttaaagc gtgaagaaaa
      661 cttcggtgca ctacctcaga caacaggatg tatcagaaat tcatctgttt aatgtttgat
      721 aaaatgggaa tatactgcgc tgatgtactt ccgcaacaca caatcgttga ataaccgaca
      781 ctttaacagg gtattgagtg aaattatgat taactctttt tataattgaa ataaattgac
      841 aactgacaag atacttaaca tcaaatcaag agctgagtgg agaaactggc ttcaacaaaa
      901 cttcaacatt gagaaggaag tttggttggt taatgccaaa aagtcatcgg gcgaactacg
      961 cattgaatac aacgatgcgg tggaagaagc actgtgtttc ggctggatag acagtacaaa
     1021 taaaaccctt gacaaagacc acaccattca gcgatacact ccccggcaaa ccaggagcag
     1081 ttattcgcaa cccaacaaag aacgactgaa acgattagcc gagaaaaact taattcatca
     1141 ttcaattctt aaaacagttc atgagattat ttctgaagag tttatttttc cctcggacat
     1201 tatggatgaa ataaggaaaa acaaaacagc ctggaataac tacctgaaat ttccagaacc
     1261 ctacaaacga atacgaattg catacattga cagtgcaaga aaacgaccgg aagaattcac
     1321 caaacgactg actaacttta ttctgaaaac acaaaacaac aaacttattt caggatttgg
     1381 cggaattgac aaatattatt aacccataca atgacctctc tcccactccc ctgatttttg
     1441 ttgcgatagt tgcctgaatg aggtatccaa gttaaatttc aggctgaagt tttgcaatca
     1501 ttttatttcg tcattttcgc ttccatttcc ctgaatgctt ttcgctggag cgaccggtct
     1561 ttttcagtta tttcagcacg acctctcagt cgggtaaaca catgcagtct ggagtctatc
     1621 atttccacat caatgtgttt gttatcatat tttattccgt tgtaaataag tactgcactc
     1681 accgggtcgg cggcatcatc tttcaccgtt ttgtacactt tcatatatat ttcatccgga
     1741 actttgatac cccgttgcaa ttccctacga gtaaggtcaa tgagtgacat caattgcctg
     1801 agggtttcct gagggcggta aacgtaccat ttcccttttt cgccgtccga catttcggtt
     1861 tcggtatatc ccagtaccgg acccaataaa ccaaccaatg aaagctgttt gtcggacgaa
     1921 actacgatgg gatcaatcag aaacactcct tccagtccgg gatcttcggt gaatagtgtc
     1981 gtagtcagca actgaataat cagcggacca ccggtggaag aaccggctaa gtaaattttg
     2041 cgataacctt tctgacttaa tttgacatat tcatcccgga tggaactttg ccagacttgc
     2101 caggtggagc tcttgaaatc ggtgtacgaa cggccatgtc cacccaacaa aacctgcgac
     2161 acataaaatg tcctggccga atcggcatat tcgcgcagtt catcccattc gaaggtggtt
     2221 gcgctgtaac cgtgagcagc aattattacc ggtgtatttt tctggacctc ggtaggattg
     2281 gcgatgtagt tcgaaaccaa tcgcttctgc aacgaatcgg gaacagcgaa tgaatatttt
     2341 ccgctgtcga gcatcgtatc atctatcgga ggtgtgggat tgcaggctgc tatcagtgtc
     2401 accaatccga atattacggg taaatatatt ttatttttca tagtggtaat atcttttttg
     2461 tgtgttttta acctgaatgc aaatcatcac tttgaggttc caatgttgta aaacaggctc
     2521 attttataaa acaccggaaa aagtgttccg ggtttggata ttccatcacc gttgatgaag
     2581 ttataaccag ccgacagacc aagtgtaaca tgtggcgcct taaatctgta aaccactccg
     2641 gtctcagccg aaaaaagcag tgatgagttg ggcaacacat caaaagcggg gttttcataa
     2701 tcggcataaa aataaccggt attcacccct gcaaaaatct ggaaaggttt tgtcggccga
     2761 aaccggaaat caactcccag cagtaggtta tcctgtttta cggcattcga taccagactg
     2821 ctgccgaacc gactggaaat gtatccggct ttcagatgaa tatgccggtt gaatagaacg
     2881 tcagatgcat aatctactcc aatcccgttt tcccaatata aatgttgggt tttttgtacc
     2941 aataaaccga cattcaactc gcttactgcc tgttgggcat tcatcgttga aaaagttccc
     3001 aacacaaaaa ttaaaagata acctattctc ttcatagttg atgcgttttg ttatttctgt
     3061 tttcttccaa attcgctgtt agtctaaatt tgaccggttc tttaagtcag cgcttccatg
     3121 ttcttttcgt tgataatgtc cttaccaccg gcttcgtaca tatcagcaat gcggtcggca
     3181 taccgttcct ccacttttcc gcgtatcacc ttcatcgtgc tgtttactaa accattttgt
     3241 tcggtaaatg gttcaccaat tactgcaatg gccgaaggaa gccagcgctc gggaaacatt
     3301 ccttcgaacc ggccaccttt tcggtattca ttgacttcgc gctggagttt ggtcaaagcc
     3361 agttctttgg cttcgcgggt ttcccatgac aattcgggct ttttgcggtg aacgtatcgt
     3421 ttgagagctt ctttgttggg tgcaataatt gccgaggtaa acggatgctg attattgtga
     3481 agcaccacct gatcgatgaa ataggaattc tcaaccatcg actcttcgat accttcagga
     3541 ctgtattttt cgccatcgct cgatatcagc aggcttttga atcttcccat aacgtacaaa
     3601 aaaccatcgt catccatgta tcccaaatcg cctgtatgca accaaccgtc ccttaacgtt
     3661 tcggctgttg ctttttcgtt tttgtaatat cctttcatta cattttcacc cctgattact
     3721 atttcgcctt tttggtaatt gggcagagca ttgtcattgt catcgcatat tttgagttcc
     3781 aaaggcgaaa caaggtaacc cgacgaccct aacttgtgtc gtttcagtcc gttggacgaa
     3841 ataatgggtg ttgcttccga taagccgtag ccctgaaaca cgggcattcc gacagcatag
     3901 aaaaaccgtt gcaaatcaat gtcgagcagg gcccctccac cgataaaata ttcaatctgc
     3961 cccccaaaac cttcacgaac tttgctgaac agaattttat caaacagcat taacaatggt
     4021 ttgtatatag cctgaatacc ctgtcctttg ttaaatcctt ccttattgta ggcgtaagac
     4081 atttttattg ccgtattgaa cagccaggta actaccggac ctttggcatg aatgcttgac
     4141 tctatatttc ttttgaagct tttggttaat gtaggcacac tgagcaataa gttgggtctg
     4201 atttcccgga tgttaatggg aatgtttttc agggtttcaa tgcccgtttt acccgacttc
     4261 acggttgcca cactcgcacc tgaagccata aaactgtaaa agccggccac atgcgcaaaa
     4321 cagtggtcga gcgggagaat aatcagtgtg cgaaaagtgc tgggaatggt catcaacgtc
     4381 agtgcctgct ctacgttggc cgtataattc cggtgagtga gcatcactcc tttgggatcg
     4441 gccgttgtgc cagaagtata gctgatagtg gcaatatcat ccggtcggat ggtcttgtat
     4501 cgttcggtta caatttccgg tgttttatct aataacactt ttccggctgc caacaattca
     4561 ctcaggggaa tttctttcgg ctgatagtcg gtttgttcgt caagcactat taccttttcc
     4621 acgtcgtgca gcgaagcaat gattttcctg attttgggca actgaccacc tgaaaccaca
     4681 atgaattttg actcggaatg gattatccgg aatactaaat cgttgctttc ctccagttta
     4741 atggacaacg gcacattaat cgccccggca tgcagaattc ccagttcgct gatgatccag
     4801 gcatttcttc cttcggataa cagggctgtt ttatcacccg gtttcacgcc ggctgcaagt
     4861 agtccggcag caaaaagcac cgcctgctgt tgtgtttcca gataagtggt gggaacaaat
     4921 tcattggtat ctttctccca aagaaatgga ttctgtgcga acttttcaac gctggagttg
     4981 aacatatcaa tgatggtggg tttcataggc ataattgaag ggtattgtta gtttcaaatt
     5041 ttcaaatgta aggaaaaatt tccaaaatta taaatattgc tatttaaaat actaattacg
     5101 ccctcgtagt ataaatttat tgtaataaag ctactccaaa gtcaaattga ataataaatg
     5161 atttacctga atctattttc aaccgttttt cagtgtaaat acaatatgtt ccgggcgaaa
     5221 aaaaaagtta ataagctata ttacaatggc ttaaaaaata aaaacacaaa attattgaaa
     5281 ctgacaacaa aaaaccggag ctgataaaac cccggtaagt tgaatggaaa agatcggata
     5341 tcatggcttt cctaactgcc aaatgttttg tgcatcgctt tgagcatacc tcccggtgtc
     5401 catcccgagg gtacaatcat gcgtctgaca gtctgcaagg gtgcaataga atctcttttg
     5461 gtggaaagaa taaaactgag cttaaaatat ttttccatcg ccacagaggt ttcgtggttg
     5521 gtaattccat acggataggc aaaatattcc acttttttac cggttatacc ttccagtttc
     5581 tcgcggggtt taacaatctg ctgcacagcc acagaatcgg tcagatattt ggtagccatg
     5641 gtatgatccc aactatgaag tcccactgta tgaccgtttt tggccagacc ggcaatctgg
     5701 tcggtggtca tgtagttctt cttgttgtag gtaatggtca tgataaagaa tacaccgtga
     5761 aagccgcgtt tctccatttc gggagcggct atggcagcat gttccacccg cgaatcatcg
     5821 aaagtgatca taaatggttt ttctggaagc ggtttgttgt aaaccaggta atcgtatagc
     5881 tcatcgggat tgatggaatg atatccactg tccttcaaca gttgcatttg tgcaccgaaa
     5941 gcagccggtg tgacggtata ttcatcgttt cggccggtgg tgatgcggtg ataacagagc
     6001 acgggaacct gcggtttggc catgatttcg gccgcagaat tcatattcac ctcactgctt
     6061 ttattttcgg caggttggga tttatccttt tgggtagaat tgctgcaggc agtaaaaaga
     6121 acaccaatca gtatcgtgag gaggaagctg taaattctat tcatcatttt attttcttta
     6181 atttttcaaa attacacaaa ttcagtaaat ctacccgaaa atgaaattta atcttaacag
     6241 attttacttt ttcaacgatg ttgcagtttc tttcctgaaa tcggggtaac gaaacaggaa
     6301 atatgccgct atcaacaaac aaaggaaaat cattacgccg aaaacggtga aactgttttc
     6361 gtcagtaagt gctgtagtca gtgaaaacac agcagtgcca agaaggataa aaacaaagtt
     6421 catcatattg agataagcca ccgtatcgcc tctttttcgc cccagatcgg cctgttgcat
     6481 catggccata cacggcacct gaaacatacc ggccataaag gcaaaaacca ccaccatacc
     6541 tacgaatgcc gggaaagaca gtggtatgaa aaccactacg agcaatgcgg caatcatacc
     6601 cgccagagcc ggcagtatca atcctttatt caccttttca cccatcacat ttccggtaat
     6661 acccgttccg atagctatgc ctaccgctgc gcaggccatg accacgccgg tcatcgaatt
     6721 cgaagtctga taatgacctt tgcagtgaat gatgatattc atttgcacca tattagcaat
     6781 aagccaaaat gccgacgagc caaatacggc actgttgacc agccggtagt tgcgggcata
     6841 gcggtaattg gccattacaa ataaaattgg attgacgatg gaagattgag attgctcttc
     6901 gggcaattcc acagcccgga tcgttttgga actataataa ccgagcacag ccacacccag
     6961 tgctatcaat ccaaacaacc cgggtgaata atgatcggat accaccgatg caacaaaagt
     7021 accgagtaaa atacccataa aagccatggt ctcgaacata ccactgccgt acgaagctcc
     7081 ctcttcgcca ccaatatcgc gtatcaaact gtatttggaa ggggaataca ggcaactttg
     7141 agtgccaagg gcaaaaactg acaatacagc cacaatgacc cactgataat aaaaagccac
     7201 acaggccagc aacatgatgg gtatctctac aagcttgagt atgcggaaaa ttttcagctt
     7261 ggaatagcgc accgccaacc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 naatttattg gaataaatta ataaaaatca gtcaaatgtt aaacaaaaaa cggttttaaa
     7441 tgttttttct ttgtaaagat ataatgttat aaatttcaaa aattcacaga aaagaaaaat
     7501 tacattgact tcatttttag ctatcttatc tcacgagtta aattttgtta aatgttaatt
     7561 taacacttga atttaactta actttgtaac ttgatgaaaa aaagtaatat ctggatatta
     7621 ataatattga tggccatcac atttggagca ctaattctac tccaggcgag gtttctgaaa
     7681 gcaacatccg aaatggctga aaagcaattt gatgacgcag tgaaacgaag cctgatacag
     7741 acagcgaata tacttgaaga gaacgaagca ctggaatacc tggcacagac tctggatgaa
     7801 aacgattaca ttcaaacgga gaataaaacc ggcaactttg gtgtaaacaa acagaaagat
     7861 acgctcaata caaactggaa aagcacatcg gcagtaagtc cgcgcatccg cttatcgaca
     7921 gggcatggta aagcgagcat tgaacaaacc tcaaaatacc tacagcaacg atttcagttg
     7981 aatttttcgc ggtcgaaaac agtgttggat caggcggtat tccggtggtt gcgtgaaaat
     8041 gagaataaag aaatcagcga gcgcgttgat tatacagacc tcaacgaaac acttgaaaca
     8101 tatctgaaaa gcaacaatgt ggatttgcct ttttattaca ccatagccga tcaaagaaac
     8161 aaggttattt atcagtgtaa taaaacgata aacacaaaag gaaaaccaaa tacagatatc
     8221 tacgtacagg aattgtttcc acacgaagat agctctcgca aagcatacat gcaggttgtt
     8281 ttccctacga aacaaagttt tatttacgaa tcattaaacc tgctgttacc ctctattggt
     8341 gtaatactgc tcacgtttgt catctttgta ctgactatct ttatcatttt cagacagaaa
     8401 cagttgaaca acatgaagaa cgatttcgtt aataatatga cgcatgagtt taaaacgccg
     8461 atttcgacca tatcacttgc atcgcaaatg ctgcaggacc ccggagtagc caaaaatccg
     8521 gacatgatga aacacgtttc gaacgtaata cgcgatgaaa caaaacgact gagtttgcag
     8581 gtagaaaaag tacttcagat gtcgatgttc gaaaaagaga aatcgactct gaaatttaca
     8641 gaaattcaca tcaataacct gataaaagat ataattggca gtttctcact caaagtaacg
     8701 agtaagggtg gaaaaataaa ttcaaatctg aaggcaaaac aggatatggc attggttgat
     8761 gaaatacatt tcaccaatgt aatcttcaat ttgatggaca acgctctcaa atacagcgat
     8821 aagccgctga tactcaatct ggaaacctgg aacgaaaaag acaacctgct gatcagcgtg
     8881 gaggacaacg gtataggaat gaaaaaagag gatttgaagc gaatatttga aaaattttac
     8941 cgcgtttcga ccggtaacct acacaacgtg aaaggatttg gacttgggct tgcatatgta
     9001 aaaaaaatgg tcaatgacca caatggtatc ataaaagtag aaagcgaacc cggtttagga
     9061 acaaaattta cgataattat accaacacta aaaaaaaaca actatgaatg acgaaaaagt
     9121 aagaattttg ctgtgcgaag atgatgaaaa tcttggcatg cttctcagag agtaccttca
     9181 aacaaaaggt tactactgcg atttacaacc tgatggcgaa gcaggttata aatcttttag
     9241 ccgcaataaa ttcgacctgt gtgtattgga tgtaatgatg ccaaaaaaag atggttttgc
     9301 tctcgctgcc gatatcagag ccattaattc tgaagtgcct atcattttcc tcaccgccaa
     9361 atcaatgaag gaagacattc tgcagggctt caagcttggt gctgacgatt atttgtcaaa
     9421 gccattcagc atggaagaat tgctctatcg gatcgaatcc atattacgcc gcgtacgtgg
     9481 taaaaaatcg aaagatgtgg ttacctacca aatcggcggt ttcacctttg acgctcagaa
     9541 acaaactctg atattcgaag gcgaaatcaa gaaacttaca accaaggaat cggagctgct
     9601 caatctgctg gctgctaatg ccaacaatat cctggaacgc aactttgcac tgaaaaccat
     9661 ctgggttgat gacaattact tcaatgcccg cagcatggat gtgtatatca caaaattacg
     9721 caaactgctg aaagacgacc agaacgtagc tattatcaat attcatggta agggttataa
     9781 actgatcatg ccacaggtga aggaagatta atctgatgga tggaattata taaaaaatgc
     9841 ggtgaattcc ttcatcgcat tttttttatt tgaatttatt ctctatctcc tcatacttca
     9901 tcatcgtcac tatcatctga ccatcgggcg tatattgatg attctcgcag gcaaacaaac
     9961 tctcgaatag ctctttcatc tctgctccgg taagcgattg accgtactga atagcggttt
    10021 gtcgcgaaag atgaaccgca attttttcac ggatagtctc cttgaccgag atttctgttt
    10081 cacgtactgc agctatcaga tctttgagca gtggcaatgt cgaagtcatt tcaatctcgg
    10141 aaggaatacc gtttatggca aattcgcttt taccgatggg ttcaatatcg aatcccaccc
    10201 atcgcaggtc cggtaagatt tgctccacaa tgtgaagatc atctctctca acttccagaa
    10261 tttcggggaa taaaacttgt tgtgtaattc ccttttgagg tcccgatttc tgtataaacc
    10321 ggtcgaagag tatcctgaag tgcgctctgt gttgatcaat gagcatcaat cccgacttca
    10381 cagtggtgag gatatattta tttttaaact gaaaaaaatc ggtgcttgta tccgggatat
    10441 ccagttcctg ctgctgttca ggttgtaaag gtgccggaaa accggcttcg gaatcagttg
    10501 gagcattctg aaaaccgggc tgccggtttg gcttactgaa atcaccatac aattgctccc
    10561 agtcgagtgt ggggcgtttt ggtctggtag tagctccaaa gggattgtaa cccgggtcaa
    10621 aattcgtttt tggaacctgt atttcggtac ttgagtcaat tacaggtata tcgggcaaac
    10681 catcctgatc aaaatcaagg ggaggcatca cctgaaattt gcccagcgat tccttcacag
    10741 aagcaaggag tatagaccag atagcctgct cgttttcgaa cttaatttcc gttttcgacg
    10801 gatgcacatt cacatcgatg gattgtgtgt caatgtcgaa ataaataaaa taattcggat
    10861 tgtcggcagg ctttatcagc tgactgtatg ccaccattac agctttatga aaatatggat
    10921 ggcgcatata gcgaccattc acaaaaaaat attgattggc tattttctgg gcaaattccg
    10981 gtcggccaac aaaaccggtg atggtcagca aacttgtttt ggcttcaatg ggcagcaact
    11041 gttgatccat tttcttccgg tacgccccaa tgaagacatg ttcaattctt gctttcaagg
    11101 atgttgcggg cagagtcatc atctcagtat cattgtcata caggaagaaa ctgatatccg
    11161 gatgaacgag tactatgcgg taaaattcat tgcggatatg ggtcatctcg gttgagggtg
    11221 acttcagaaa gcgtcggcgg gcaggcacat tgaaaaacag acttttaacg gctatgttgg
    11281 ttcccttgtc acattgcact gtttcctgac gaaaaacccg cgttccggct atttcaatca
    11341 atgttcccaa ttcttcgttt tccctgcggg ttcgcagttc tacctgagcc acagccgcta
    11401 tcgatgccag ggcttcgccc ctaaacccca tggtgcgaag tgaaaataaa tctgctgcgt
    11461 ctttgatttt tgatgttgcg tgacgctcaa aggacatacg ggcatctgtt tcgctcattc
    11521 ccactccatc gtctatcacc tgaatgagtg tactaccacc gtcttttatc agcacttgaa
    11581 tatttttagc accggcatca atgctatttt cgaccagctc cttgattaca gatgctggtc
    11641 gctggatcac ttctccggca gcaatctgat tggcaaccga atcgggtaat aaatggatnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gtcgtttaca aactggcgta
    11821 tgcgttgctc atctgacttt ttacacacca gcagtacatt gtccgattca gccacaatat
    11881 atccgttcag atcatgtatc acggctagtt ttccacttgg aagagctaca atattgtctt
    11941 cgctttcgta aaatatagct tcacatttca gcgtcacatt atttttttca tcttttgccg
    12001 attgatcata aagcgaaccc caggttccga ggtccgacca tccgaaatcg gcacagagca
    12061 catgtacacg ctctgctttt tccatgattc cataatcgat ggagatattg ggacaggaag
    12121 ggtacatttc gttgatataa tcctgctctt tatctgtgtt gtagtaattt tttccttcgt
    12181 taaatttagc ggcaacatcg gggagataag ttctgaatgc gttgtcaatg gcctgcaaat
    12241 tccaaagaaa gatgcccgag ttccagaaaa attctcctgt ttcgaaaaaa atctgagcca
    12301 gttcggcatt gggtttttca gtaaaggttt tcacttttcg tggatttgtt gagccctcat
    12361 ccacctgaat atatccataa cctgtttcgg gacggctggg tttaattccc aaagtgagga
    12421 gactgtcgtt tttatccaca aaatccaatc ccgtttttat tgtttcaaga aattcggctt
    12481 cttttgtaat gaggtggtca gatggagcta cgataatatt tgctttatct gtaattgcct
    12541 taatccgctg aattgcataa gcaatacagg gtgctgtatt tcttcgggct ggttctaata
    12601 gtacctgttc gggtttaatt tcaggcaatt gctgcaaaat caattccctg taaatcacat
    12661 tggatacaat taaaatattg gctgcaggca caattgccct gaaccgatca taggtcattt
    12721 gtatcaatga acgcccagta ccaaagaaat caaggaattg tttgggttta tcgttacggc
    12781 tgaagggcca aaaacgtgaa ccaagcccac cggccatgat tacacaataa ttattttcca
    12841 tatatttatg atttactgat aaatacaaat tctaaaaacg ttccactaaa gtaagattat
    12901 tttttagatt aagcaaatat atcggaaata aaatagcaac ggttcaacga tcaataccaa
    12961 caacctgaaa tatgatgttt tataatgact tttgtcagtc tgcgaatttc gctggaatac
    13021 cagttgtgaa ttacttttgc tttgcaaagg tcaatattcc ccttttatct tgtaaaaata
    13081 aataaaggta caatcttgcg agtgtacctt tacttttatt aaatgtccgg aggactattc
    13141 tgtttccaca tcttcatcct ggctgatatc aagcacgttt ttgcgtgcat ccatcatctt
    13201 ttcgtattcg tcacgcgaac ctacaatgat tttttcgtat tcacgcagac cggttccggc
    13261 aggtatcaga tgaccacaaa tcacgttttc tttcaaccct tcgagcttat cgatttttcc
    13321 gttgatagct gcgtcattca gtactttggt tgtttcctgg aatgaagcgg ccgacatgaa
    13381 actgttcgtt tgaagtgctg cacgggtgat accctgaagt acctgactgg atgtggcagc
    13441 taccgcgtcg cgggttagaa ccagtttaag gtccttgcgt ttcaatacac tgttttcatc
    13501 cctcagttta cgggcggtaa taatttgtcc ggccttcagg ctttctgaat cacctggatc
    13561 aaggattact tttttacccc aaatacgatc attttcttcc atgaattcgt gtttatcaac
    13621 cacctgtttt tcgaggaagc gtgtatcacc ctgatcttcg atcaatactt tgcgcatcat
    13681 ctgacgaaca ataacttcaa agtgcttgtc attaattttc acaccttgca gacgataaac
    13741 atcctgtact tcattcacaa tataatcctg aacggcagtc ggccctttga tagccaaaat
    13801 gtcggcagga gtaatggctc cgtcagatag cggtgtaccg gcacgaacaa agtcattttc
    13861 ctgaacaagt atctgttttg acagcggaat aagatatttc ttcacttcgc cggttttgga
    13921 tgttactgat acttcgcggt taccacgttt gatttttcca aagttgattt caccgtcaat
    13981 ttcagctaca accgaaggat tggaaggatt acgggcttcg aacaattcgg ttacacgcgg
    14041 aagaccaccg gtgatatcac cagctttacc aatagcgcgg ggaattttaa caagcatctg
    14101 accagctttg atggtactac catcgtcaag caccaggtga gcgcctaccg gtaagttgta
    14161 atttcggata ttaccttcta catcaacaac attcgcagtc ggtactttcg tcttatcttt
    14221 tgcttcaatg ataatctttt cacgaagtcc ggttgcatca tctacttctg ttttataggt
    14281 aacattttca atcacatctt cgaattcgat cttaccttct gtttcggcaa tgattacagc
    14341 attgaatggg tcccagctac agatgaactg acctttctta cccaaatcgc catgtttggc
    14401 atacagtgtg gaaccgtaag gaacattctg agtggtgagg ataattcctg tattcggatc
    14461 cacaacacgc aattcggtca aacggctaac taccacacga cagggtttgc cttcttcatc
    14521 gacagcatct acagtacgaa gttcatcaaa ttccaggcga ccatcgtaac gcaggattat
    14581 tttggattcg gctgcaatgt tggatgcaat accaccgacg tggaatgtac gaagtgtaag
    14641 ctgcgtaccc ggctcaccaa tggactgagc ggcaataacg cctactgctt ctccctggca
    14701 taccattttg ccggtagcca gattgcgacc ataacatttg gcacaaacac cttttttgga
    14761 ttcacaagtc agtactgagc gtatttctac acgttcaatc ggggaatcct gaattttacg
    14821 ggcagcaact tctacaatct gttcacccga agttaccagc aattcacctg tcaacgggtg
    14881 gtaaatatca tgtacggaca cacgtcccaa aatccgttcg tagagcgacg aaattacatc
    14941 ttcgttattt tttatttcgg tagcaaccag tccgcgtaat gttccacagt cttcttcgtt
    15001 gatgattaca tcctgtgaaa catccaccaa tcgacgggtc agataaccgg catcagccgt
    15061 tttaagagct gtatcggcca accctttacg ggcaccgtga gtggatataa agtattcgag
    15121 taccgatagt ccctctttaa agttcgacaa aattggattt tcaataatct gtcctccttc
    15181 agcacccgat ttctggggtt tagccatcag accacgcata ccggcaagct gacgaatttg
    15241 ttccttcgaa ccacgggcac cagagtcaag catcatatac atggagttaa agccctgacg
    15301 gtcttcggcc agttgtttga tcaaaacatt tgacaatttg gtattaacgt gtgtccaggt
    15361 atcaatgatc tggttataac gttcacgcgg tgtaatgaaa cccaggttgt agttattcat
    15421 aatttcttcc acttcagcat ttccttcctg tacgagcgtt tctttttctt ttggaataat
    15481 aacgtcgctc aggttaaagg acaaacctcc gcggaaggcc atataataac caaggtcttt
    15541 gatatcgtca aggaaaagtg cagtacgtgc aaccccgcag gtatcaatca cgcgtccgat
    15601 gatatcacgc aacgattttt tggataacag ttcgttgatg aatcccattt cttcaggtac
    15661 gtgcttattg aaaagaatac gtcccacagt agtttctatc aaacgaacgg ttaattctcc
    15721 attttcgtcg agatctttag ttctcacttt gataagcgaa tgaagtaata ctacgccttc
    15781 attgtaagca atttcggctt cttcgggcga atagaatatc agtccttcgc ccttggaacc
    15841 cggccgtggt ttggtaatgt aatacaaacc aagcaccatg tcctgtgatg gtacggtaat
    15901 aggtgcacca tttgaaggat tgagtatatt gtgagaagcg agcatcaaca tttgggcttc
    15961 cagtacagct tcatttccaa gtggcaaatg cacagccatc tggtcaccgt caaagtcagc
    16021 attgaaagca gtacaggcaa gcgggtgaag ttgtatggct tttccttcta tcattttggg
    16081 ctggaaagcc tgaataccca aacggtgcaa tgtaggggca cggtttagca atacaggatg
    16141 tcctttcatt acgtattcca gaatatccca gattacaggg tctttgcggt caataatctt
    16201 tttggccgat tttaccgttt ttacaattcc gcgttctatc agtttacgaa ttacaaatgg
    16261 cttatacagt tcagctgcca tatcctttgg caaaccgcac tcgtgcattt tcaattcggg
    16321 tcctaccaca attaccgaac gtgccgaata atccacacgt ttaccgagca ggttctgacg
    16381 gaaacgtccc tgtttacctt tcaaactgtc ggagagagat tttaacggac gattggcatc
    16441 tgtttttacg gcactcgatt tgcgtgaatt gtcaaataaa gaatccaccg cttcctgaag
    16501 catacgtttt tcattgcgca gaatcacttc cggtgctttg atctcaatca gtcgtttcag
    16561 tcggttgtta cgtataatca ccctgcggta caaatcattg aggtcggaag ttgcaaaacg
    16621 tcctccatcc agtggaacca acggacgcaa ttcgggtgga ataaccggaa cgattttcat
    16681 aatcatccat tccgccttat ttctagtttt cgaggcacgg aacgattcta caatctgcag
    16741 tcgtttaagc gcttcggttt tgcgttgttg agaggtatcg gtattggctt tgttgcgcag
    16801 atcgtaagaa agtgtatcca gatcaagtct tgtcagcaaa tcatagattg cttctgcacc
    16861 catctttgcg atgaatttat tggggtcggt atcttccagg agctgattgt cgcgtgggag
    16921 tgaatccagc aggttcaggt attcctcttc tgtgagcaat tcgttattgg taatattttc
    16981 accgttatcg agaataccgg cctgaataat cacatatttt tcgtaataga tgattgcatc
    17041 cagttttttg gtaggcattc ccagcaaata accgatttta ttgggcagtg agcggaagaa
    17101 ccagatatgt gccacgggca caaccagctg gatatgtccc atacgctcac gacgtacctt
    17161 cttttcggtt acttccaccc cgcagcggtc gcaaactata ccgcggtaac ggatacgttt
    17221 gtatttaccg cagtgacatt cgaaatcctt ggtaggaccg aaaatccgct cgcagaacaa
    17281 tccgtcgcgc tctggtttat aggtacggta attaatagtt tcaggtttca gcacttcgcc
    17341 gctcgaaagt cgcagaattt cttccggcga ggccagcccg attgagattt tattaaaact
    17401 actctttgtt ttattatcgt ttttgaaagc catgtttctt gacaatttat ttatttacat
    17461 tttacatttt ccacgtgagt gaaaaagttc tctaaattga ttttcccata attttttatc
    17521 ccggtcagga gaaaattagg gggcttttct gctttattcc aggttgatgc ttaatccaag
    17581 acctctcaat tcgtgtaaca acacgttcag agattccggt attcccggtg ttggcatcgg
    17641 atcacctttt actattgctt cgtatgtacg tgcacgaccc atcacatcat cagacttcac
    17701 ggtaagtatt tcctgaagta tatgtgatgc accaaaagct tcaagtgccc aaacctccat
    17761 ttcaccaaaa cgctgacccc caaactgagc tttacctccg agtggttgtt gggtgatgag
    17821 tgagtacgga ccgatggaac gggcatgcat tttatcttct accatgtggc ccagtttaag
    17881 catataaatg atacctactg tggctgtctg gtcgaaacgt tctccggttc caccgtcata
    17941 gagataagtt ttaccataac gcggaacacc ggctttgtca caccattgat ccaggtcatc
    18001 gagcgaagca ccatcaaaaa tcggagttgc aaagcgtaca cccaattctt taccagccca
    18061 gcctaaaaca gtttcgaata tctgtccaag gttcatacga gaaggtacac ccagtgggtt
    18121 caaaacaatg tctaccggtg ttccgtcttc aaggaacggc atatcttcct ggcgaacaat
    18181 ttttgaaaca atacctttat tcccgtgacg accggccatt ttatcaccca cacgtatttt
    18241 acgttttttg gcaatatata ctttagccag ctgaacaata ccgttgggaa gttcgtcacc
    18301 gatggtgata ttgaacttct gacgtttgat ctgagcgtct aattctttgt atttatgtaa
    18361 ataattcagt atcaacagac ggatgaggtc atttttatga ctgtcagtag tccatttacc
    18421 aagctgcact gtagtataat ctacttcccg cagccgttcg gctgtaaact tactgccgcg
    18481 tgtaattata tcagcaccca gatagtcttt tactccagcc gaggttttat cttcaatgat
    18541 tatcagcagt ttttcaatca accgttcctt gagtgcagct gattgagctt caaattcctc
    18601 atccagttta ggcaacacag ctttatcggc cagtttcgat ttacggtttt tctgagcttt
    18661 tgagaataat cgtttattaa taacaacacc agaaagtgaa ggtgtggctt tcagcgatgc
    18721 atctttcaca tcaccggctt tatcaccaaa gattgcacgg agcagttttt cttcgggtga
    18781 aggatccgat tctccttttg gagtgatctt accaataatt atatcaccgg gttcaatacg
    18841 tgctccgatg cggataatac cattttcatc aagatctttc gtagcttctt cgctcacatt
    18901 tggtatatca gaggtaaatt cttcgatacc gcgttttgtc tcacgcactt cgagcaattg
    18961 ctccaccacg tgtatggaag tgaaaatatc ctcacgcacc acccgttcgt tgattacaat
    19021 agcatcttcg aagttgtaac ccttccaggg catgaatgcc actttcaggt ttttgcccag
    19081 ggccagttca ccgttatggg tagagtatcc ttcggtcatt atctgtccgg cagtcacttt
    19141 ctgacctttc gtaacaattg gacgtaaatc aatggtcgta ttctggttcg ttttcaggaa
    19201 ttttggcaat tcgtaagtct taacagctgt atcaaagctt acaaattctt cttcttcgga
    19261 gcggttatac cgtactttaa tggtgttggc atcaacaaat tcaacttcac cttcaccttc
    19321 agcggtaatc tgcgtacgcg agtcgcggat caactgacct tcgatacctg tacccacaat
    19381 cggagcatcg caacgaagta gcggaactgc ctgacgcatc atgtttgagc ccatcagtgc
    19441 acggttggca tcatcatgtt caagaaacgg aataagcgca gcagcaatag aagctatctg
    19501 cgagggagct acatccatca gattaacatc cgatggtgga acaaccggga aatcagcttc
    19561 gagacgggtt tttaccactt tgcgtataaa actgccatca tcattgagcg gagcattacc
    19621 ctgagctacc accaggtctt cttcttcttc ggcagtgtaa tatttaattc cttcaggcga
    19681 caaatccaca atcgaatttg taaccgtgcg atagggtgtg gatataaatc ctaattcatt
    19741 aatcttcgca taaatgcaaa gtgatgagat aagaccgata ttcggacctt cgggagtttc
    19801 gataggacaa agacgtccgt agtgtgtata atgtacgtca cgaacctcaa atccggcacg
    19861 ttcacgcgac aaaccgcctg gcccgagagc cgacaagcgg cgtttgtggg taatctcagc
    19921 caacggattt tgttgatcca tgaattgtga gagggcattc gttccaaaga atgaatttat
    19981 caccgaggag atgctcttgg cattgataag atcaatggga gtaaacacct cgttgtcacg
    20041 cacattcatg cgttcgcgaa tggtacgggc catacgtgcc aatcccacac caaactggtt
    20101 gtgcagttgc tcgcctactg ttcgtacacg gcggttactt aagtggtcaa tatcatcaac
    20161 ttcagctttg gaattgataa gttcaaccag gtacttgata atttcaataa tatcttcctt
    20221 ggttaatact ttaatatctg aattggtaga taaatttaat ttgcgattaa tacgaaaacg
    20281 gccaacatca ccgaggtcgt agcgtttttc agagaaaaac aaattattaa ttacttctct
    20341 tgcagaggag tcatcggccg gttcggcatt ccgcaactgt cgataaatat acagcacggc
    20401 ttctttttcc gaattgctcg gatctttttg caatgtattg taaatgatgg cataatcagt
    20461 ctgattagca tcatctttgt gaatgagtac tgtctgtgta cccgattcaa taatttcttc
    20521 gatatgactg ttctccagaa tggtttcgcg gtcaataatc actttatttc gttcaatagt
    20581 aactacctct ccggtatctt catccacaaa gtcttcgttc caggtatgca gtacacgtgc
    20641 agcaagtttt tttcccagta cttttttaag gtttgtcttg ttaaccttaa cctcttcagc
    20701 cagattaaaa atttccagaa tgtccttatc gctttcaaaa ccgatagcgc gtaagagtgt
    20761 ggttaccggt aatttctttt ttcggtcgat gtatgcatac atcacattgt tgatgtcagt
    20821 tgcaaactca atccatgaac ctctgaatgg aataatcctg gcagaataaa gtttagtgcc
    20881 attggcatgc atgctctgcc caaagaacac acctggtgac cgatggagct gcgatacaat
    20941 aacacgttcg gcaccgttga taacaaatgt gcctttggga gtcatatatg gtataggtcc
    21001 gagatataca tcctgaatta ctgtatcaaa atcttcgtgt tcgggatcgg tacagtagag
    21061 tttcagttta gccttgagag ggacactata ggtcaatcca cgctcgaaac attcttcaat
    21121 ggaataacgg ggtggatcaa taaaataatc gagaaactct aatacgaaat tattacgtgt
    21181 atcggcaatg gggaagtttt cgttaaaaac tttatataaa ccttcggttt ttcttttttc
    21241 gggcgccgtg tccatttgaa aaaagtcctg gaaagatttt aattgta
//
LOCUS       DAOPZK010000018        21212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2550_length_21212_cov_22.671173, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000018 DAOPZK010000000
VERSION     DAOPZK010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21212
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_21212_cov_22.671173"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..86)
                     /locus_tag="PKH68_02090"
     CDS             complement(<1..86)
                     /locus_tag="PKH68_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNX11755.1"
                     /translation="MSSKKNFVIDTNVILHDYNCLHNFEENDV"
     gene            complement(338..676)
                     /locus_tag="PKH68_02095"
     CDS             complement(338..676)
                     /locus_tag="PKH68_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX11756.1"
                     /translation="MRKFTLILTFILCGIAGIQSQKNSTLFDKNTTETSNTQSTKTSE
                     SDVVPKFTVTGKTLNVSNMDNGVQIDIYSALGAKVLTTVYNGTSISLVNLNKGIYIVR
                     AGKFTQKIML"
     gene            945..2591
                     /locus_tag="PKH68_02100"
     CDS             945..2591
                     /locus_tag="PKH68_02100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX11757.1"
                     /translation="MNKIEPEYIFETSWEVCNMVGGIYTVLSTRSETLYNHFKDKLIF
                     IGPDIWKETESPYFDPSEELLSEWREHFTRTTGLPVRTGRWKVPGNPVTILVDFTPLT
                     YRKNEIYGHIWEKYGVDSLHGYGDYDESSMFGYATGLVIESYYQYHNLSAENNVVAHF
                     NEWMTTFGAFHIQEAVPEIATLFTTHATSIGRSIAGNHKPLYDYLTEYNGDQMAYELN
                     MVSKHSTEKKAAHIVDCFTTVSDITARECTQLLDKTPHIVTPNGFDDTIVPTGKLFDD
                     KRKTARAKLKEVTETLIGYKLNDDALFISTAGRYEYKNKGIDTFIESLKKLTNHKELN
                     REVVAFIMVPAHIKGPRVDLTAKLNGSDIAIDYWNKHTTHELYDYQYDNVISALRWFH
                     FTNLQNEKVKVIFVPSYLNGNDGIFNMGYLDLLIGMDVSIFPSYYEPWGYTPLESVAF
                     SVPTITSDLSGFGQWVKSHYDTRHGVAVVHRSDYNQAQISEEIGNILAQFANYSAAEA
                     ENVRKQALETSKQALWNEFIQYYYQAYDIALTNRNKRIKH"
     gene            2777..5341
                     /gene="glgP"
                     /locus_tag="PKH68_02105"
     CDS             2777..5341
                     /gene="glgP"
                     /locus_tag="PKH68_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNX11758.1"
                     /translation="MKLKANNTNDVPWRNIIIKSNLPENLNKLDEMAQNIWWSWDSEA
                     KKLFREIDRPVWKEAESSPVQLLNLLSYDKLDALANNEEFIAKVDNVYNKFKAYLNEP
                     MNTSRPSVAYFSMEYGLNEVLKIYSGGLGVLAGDYLKEASDSNVDLTAVGFLYRYGYF
                     TQSLSVDGEQISVYEPQNFNTLPIKQVMDENNLPLEVEVPYPGRTVYAHVWKVSVGRV
                     ALYLLDTDFDKNNDYDRSITHQLYGGDWENRLKQEIMLGIGGILTLKKLGIKKQIYHC
                     NEGHAALINVQRLVDLIQDEHLTFNEAIEVVRASSLYTVHTPVPAGHDSFDEGLFGRY
                     MSEYPAKLQISWGDFMDMGRENPGDTNEKFNMSVFACNTNQEVNGVSWLHGEVSKKMF
                     APIWKGYFPEESHVNYVTNGVHLPSWASSEWKALYEKYFTKAFYEDQSNLKIWEKIHQ
                     VPDQEIWNLRMSLKNKLVEYIRNQFQESWLKNQGDPSRIVSILDSINPNALIIGFGRR
                     FATYKRAHLLFTDLERLEKIVNDPIHPVQFLFTGKAHPADGAGKGLIKRIVEISHMPQ
                     FLGKVIFLENYDMRLAKRLISGVDIWLNTPTRPLEASGTSGEKAQMNGVLNFSVLDGW
                     WYEGYRKNAGWALTEKRTFQNQEFQDKLDAITIYNILENEIIPLYFAKNTKGYSPEWI
                     QYIKNSIAQITPCFTTKRMMDDYFERFYNKLAKRGGLLKANDFKVAKELAAWKETLSE
                     GWNNIEVVSVSTTEKFITNPQVGENYEANVVIDTKMNNEKNIGIEMVTISTDRNNVDH
                     FFKASEMNLVKTEGSKMYFNLTFKLHQAGVYKTSYRMFPKNELLPHRQDFATVRWF"
     gene            complement(5466..6491)
                     /gene="tdh"
                     /locus_tag="PKH68_02110"
     CDS             complement(5466..6491)
                     /gene="tdh"
                     /locus_tag="PKH68_02110"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNX11759.1"
                     /translation="MKALVKLRPEKGIWMDNVPVPEIGINDVLIKIKKTAICGTDLHI
                     YKWDDWSQKTIKTPMTIGHEYMGVVVDKGAGVKNVKIGDRVTGEGHIACGHCRNCRRG
                     KLHVCENTVGIGVNRDGAFAEYLSLPEVNVVHLDDRIPDEIASIMDPFGNATHTALSF
                     PVLGEDVLITGAGLIGTMATAICKFAGARNIVVTDLSEYRLEIARKMGATYTVNGKNG
                     ETVEGARKELKLRGFDVGLEMSGAPVAFREMIDNMYNGSKIALLGILPNSTTVDWNEI
                     IFKALTLKGIYGREMWETWYKMQQMLLTGLDLSPVITHRFSIDDFQQGFDVMESGQCG
                     KVILKWD"
     gene            complement(6588..7778)
                     /gene="kbl"
                     /locus_tag="PKH68_02115"
     CDS             complement(6588..7778)
                     /gene="kbl"
                     /locus_tag="PKH68_02115"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNX11760.1"
                     /translation="MYQEFKSHLQTELTNIWEAGLYKNERIIVSPQGAVIRVADGKEV
                     LNFCANNYLGLANSQTLKDAAKEAIDSHGYGVSSVRFICGTQDLHKQLEASISRFFGT
                     EDAILYAACFDANGGVFEPLLNEEDAIISDALNHASIIDGVRLCKAQRYRYANADMDD
                     LEEQLKKSQAQRYRLIVTDGVFSMDGNVAPLDKIYELAEKYNAMVMIDESHSAGVVGR
                     TGRGVTERYDLRGKIEIITGTLGKAFGGAIGGFTTGKKEIIELLRQRSRPYLFSNSIP
                     PMVAAAGIKMFEMIDATNDLQDKLHANTEYFVKRMKDAGFDIKPTESAICAVMLYDAK
                     LSQDIATRLLDEGIYVTGFYYPVVPKGQARIRVQISAAHEPQHLDTCIAAFTKVAKEF
                     GVIK"
     gene            complement(7911..8516)
                     /locus_tag="PKH68_02120"
     CDS             complement(7911..8516)
                     /locus_tag="PKH68_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNX11761.1"
                     /translation="MKRTNYSALIFFITTVVLAVMLIQTKKAERKAIYDSTTVLNKIV
                     FVQELTLVRYNYAGVISFKDYMTIMNFQVPLTEKFFLLKYNGYIKAGVNLKGATVKVD
                     GKSVVVQLPKAQIFETVIDEKSIRVYDESMNAFNPISINDYNKAISKEKLTITKSAVS
                     QGILDDASRQAHLIITSILTEMGYEKIQVVDVKSLILPGKE"
     gene            8957..9667
                     /locus_tag="PKH68_02125"
     CDS             8957..9667
                     /locus_tag="PKH68_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNX11762.1"
                     /translation="MIFPFKKILAILVIIAITIPCISGSKKPSKTTESEPIPITVLLQ
                     PTIESVYDSLHLDQKGLSKVAFDYAINGYKELKQKGMLHNDSIITIVDFDQPSYNKRM
                     YIIDVKNFKMLFNTWTAHGRNTGQETAKYFSNRPQSYQSSLGFYLTDASYNGKNGYSL
                     KLIGLEKENSNALNRAIVLHGAPYVSQRMINAQGYIGRSQGCPAVPQELNKPIIEKIK
                     GGSCFFIYNNAYHFDNRI"
     gene            9825..11090
                     /locus_tag="PKH68_02130"
     CDS             9825..11090
                     /locus_tag="PKH68_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNX11763.1"
                     /translation="MAPLAERMRPQTLDQYIGQKHLVGNNAILRSMIDSGHIASFILW
                     GPPGVGKTTLARIIANQLERPFYTLSAVSSGVKDVREVIEKAKSSRFFSQANPILFID
                     EIHRFSKSQQDSLLGAVENGTVTLIGATTENPSFEVITPLLSRCQVYVLKPLDDNDLL
                     ELADRVIKNDVELKKRNIFIDEKEAMLRFSGGDARKLLNIIELVVNSESDPEVHITNE
                     TVTNRLQQNPMAYDKDGEVHYDIISAFIKSIRGSDPDGAIYWLARMVAGGEDPKFIAR
                     RLVISAAEDIGLANPNALLLANTCFDALHKIGWPEGRIILAETTVYLASSPKSNSAYL
                     AIDEALALVEQTGNLPVPLHLRNAPTKLMKNLDYGKGYKYAHDFENNFVNQQFLPDKI
                     KDKRIWKVQENAAEKKMNEWLRFLWKGKY"
     gene            11145..12017
                     /locus_tag="PKH68_02135"
     CDS             11145..12017
                     /locus_tag="PKH68_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX11764.1"
                     /translation="MKKISILLLSLLSLMGFAQKSAYQVTSPVELNVMTFNIRLDSEK
                     DGLNNWKNRKDIVANTIRYYEADIIGTQEVLHNQLLDMEALLPGYSSIGVGRLDGMEK
                     GEYSAIFYNHNKLTETGSGYFWLSENPLAVGNKGWDAACERIATWAKLKDNRTGKIFF
                     VLNTHFDHVGKKARTESAKLILEKVKLYAKKLPVIVTGDFNSTPESETIKILTDTSNP
                     LHLTDSHAVSPTVEGPDWTFHDFGRLPLKERELIDYVFVKNNVNILTYKVIDEKVDDK
                     YTSDHNPVLVRVQF"
     gene            complement(12092..12835)
                     /locus_tag="PKH68_02140"
     CDS             complement(12092..12835)
                     /locus_tag="PKH68_02140"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNX11765.1"
                     /translation="MIYFIADAHLGSRLVKDPRKHEMRLVNWLDRVKKDAEAIYMLGD
                     MFDFWFEYKSVVPRGFARFLGKMAELTDSGIEIHYFTGNHDVWTFGYLEREIGLIVHR
                     GNETINIHGKKFFLAHGDGLFVDDKGFKLIRSIFHCKICQKMFQWIPPDIGQNFGYNW
                     SKNNRLKIQEIENKYQGEENETLVIFAKNYVKSHEVDFLIFGHRHIELDLQLQNQSRI
                     IILGDFVGIFSYGIFDGENFWLETDTDEN"
     gene            complement(12889..13203)
                     /locus_tag="PKH68_02145"
     CDS             complement(12889..13203)
                     /locus_tag="PKH68_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNX11766.1"
                     /translation="MNEFLEREEKIVRMLKTVFDPEIPVNIYDLGLIYRIEPQEDNSV
                     DIDMTLTAPNCPAVDFIVEDVRMKVESIPGISEARINIVFDPPWDKDMMSEEAKLELG
                     FL"
     gene            complement(13264..13677)
                     /locus_tag="PKH68_02150"
     CDS             complement(13264..13677)
                     /locus_tag="PKH68_02150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNX11767.1"
                     /translation="MTIEKYKYELEFKVRDYECDLQGIVNNAVYQNYLEHTRHEFLLN
                     HQVSFSDLHDQGIDAVVARIEIAYKYPLRSGDKFVCKLYVVKEGVKYVFHQTIYRKPD
                     NQLCVTAKVATVVTIDGKLTADFELFDRLVEQTNA"
     gene            complement(13689..14315)
                     /locus_tag="PKH68_02155"
     CDS             complement(13689..14315)
                     /locus_tag="PKH68_02155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNX11768.1"
                     /translation="MNIDDYILQHIDPEPEYLTRLNRETWIRMLNPIMCSGHLQGRVL
                     SMLCKMIQPVHALELGTFTGYSALCIAESLPENGELDTIESDDELEEFILRNFEKTPF
                     EDKINLLIGNALDIIPTLDKTYDLVFIDADKREYEAYYEAVLPKIRQGGFILADNTLW
                     SGKVVEKVHQNDQQTLGILRFNELIATDNRVEKVILPLRDGLTLIRKK"
     gene            complement(14315..15538)
                     /locus_tag="PKH68_02160"
     CDS             complement(14315..15538)
                     /locus_tag="PKH68_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNX11769.1"
                     /translation="MKSYKTHTLSNGLRLVHLPGNSLVSYCGVIINAGSREEKKGEYG
                     MAHFIEHMLFKGTEKRTSRQIINRLEDVGGELNAYTSKEETVVYAGFLNEYTERSIEL
                     IADIVQNSVFPQNEIDKEIEIIQDEIQSYNDSPSELIFDDFEELLFKPNPMAHNVLGN
                     KKNIEHFDTARALEFYHSHYLPTEMVFFSLGAVDFDKIVRWCQKYFIESERNRKVNTR
                     IAPPVQPAEQKVVKRNTHQIHCMIGTRAYDINHPDRMILYLLNNILGGPGMNSLLNLS
                     LREKHGLVYNVEAEYQAFTDCGWWAVYYGCDPENADKCENLVRQELASLCLNPIKEHK
                     LNKYKLQLMGQIAISSENKENLALSLGKSYLRFGKFDGMDEVRKKINTITSEELQRVA
                     IEIFNPQNLTLLKYN"
     gene            complement(15636..17231)
                     /locus_tag="PKH68_02165"
     CDS             complement(15636..17231)
                     /locus_tag="PKH68_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX11770.1"
                     /translation="MKKAMILALLVGVVTFVSAGNKMATDYYEAGDYATAKTHFLAGN
                     VDAMDSYYLGQIYFKQNKMDSAKYYFEKGLQIDPANSYNTVGLATVKMATDLKGADNM
                     LKAVSKLKQYKKDAGIFTAIAEAYQLNNNDLQAKAFLAKAKKANKKSALPYIIEGDKF
                     LSQKNNGEAAVRYENAIYFDPNSKAALLKLANLYETTRTQLAFEYLRKAIDVDPQYAI
                     AYSNLGEMSYRKGFYPQAMEAYEKYLGLVKPTPKDYAQYATILYFNKQYSKALEAISK
                     APQDYVMDRLKMYSLNELGNLSEAEAIGAKFMKETPVKDVISLDYTTYAKVLAKAKKY
                     NDAAANYEAAFKLDTTKLQYVSEAAKAYERAKNYPKAIENYQHAISSNPGHSMADIYT
                     LGGAYYNAGLDTVATKDKAARKKYLLKADSTFKSMSVKFPDHYLGYLSSARTNSALDP
                     ETTEGLAKPYYEKALEVMLPSQNERKAEILECYRYLGYYYYVKNELVQSKTFWNKVLE
                     IDPTDAVSLQVIKSIDSSLKKGK"
     gene            complement(17313..18251)
                     /locus_tag="PKH68_02170"
     CDS             complement(17313..18251)
                     /locus_tag="PKH68_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX11771.1"
                     /translation="MKNFHILIVLLLSIAFLSSCGGKKEVKNENTTTSGYIKIAVDQT
                     MQKVIQNEIDVFEGLYPAKIEPVYTTETEAIELLKKDSVRLIVTARKLTEGEMIFFEE
                     KKFQPKNVRIAIDGIALIIHPQNPDSIINVENVKKILTGEYTKWSEIYPGSKLGKIQV
                     VFDNTKSSTVRYAIDSICRDKPISQNLSALELNEEVVDYVSKSPAALGFIGVNLISDD
                     ADTTAIDFTKKIQVMRVSREDKADRYNSVQPYQYYIYTHQYPFVRDIYFIVNDPRGEL
                     PRGFSRFVASDKGQRIIKRSGLLPSTMPISTVHIKD"
     gene            complement(18579..19406)
                     /locus_tag="PKH68_02175"
     CDS             complement(18579..19406)
                     /locus_tag="PKH68_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX11772.1"
                     /translation="MAKDVNLTSQDWIDLIFEGKNKAYGAYTLRKSSPKRHLVAFLGV
                     GTVAVLTFLSASLINMYNQNRKKELMTEVAQMSNINMKKAEVPKENIEKQFKAPPPPE
                     LKSSMKFTAPIIKKDEEVKEENEVKSQEEITESKVAISVADVKGTNEETGVDIATLQE
                     HKVIVAEEEPEKVFEVVEQPPSFPGGDAALMEYLNNNISYPAIAQENSIQGKVTCSFV
                     VGKDGSIQDVRVERSVDASLDKEAVRVIKSMPKWIPGRQGGNAVKVKYYLPVSFRLQ"
     gene            complement(19432..20079)
                     /locus_tag="PKH68_02180"
     CDS             complement(19432..20079)
                     /locus_tag="PKH68_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX11773.1"
                     /translation="MSAEIQQKDSGGKKKAGHMKKLSTRIDFTPMVDMIMLLLTFFML
                     ATSMSKPQTMEIGMPTKDKVEDKDKNEAKASEAVTIYLSKDHKVYYFMGIPELDKPDF
                     LKQTDFSPEGLRKILIQKNAAIVAKVNELKDKKLKLQVTQADYEKQLSTLKSSPGTPV
                     VVIKPLDNSTYNDMISALDEMLICNVGKYAITELDANDKTLLKNSKVQYEEAPKN"
     gene            complement(20142..20759)
                     /locus_tag="PKH68_02185"
     CDS             complement(20142..20759)
                     /locus_tag="PKH68_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNX11774.1"
                     /translation="MPKIKLPRKDTWIDMTPMSDVMILLLTFFMLTSTFLKPEPVKVN
                     TPGSVSEIKIPEKNIFSVLISPDGKVFLDVDNPDDRIAILERVAAEYQINLTEKEKKD
                     FSIATSFGTPIQQFKEFMAIPGEKQKVELANYGVPTDSTDNQFQAWVKAAREVKGKDM
                     VIAIKADQGTPYKKIKAVMGSLQDLRENRYNLITSLREVSAPKPD"
     gene            complement(20865..>21212)
                     /locus_tag="PKH68_02190"
     CDS             complement(20865..>21212)
                     /locus_tag="PKH68_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNX11775.1"
                     /translation="TINKTIEEATALEMPTMNQNLPVIATFTALGTLTGLFGTVLGMI
                     RSFSALAGEGGTDSAALSVGISEALVNTAFGILTGALAVISYNYFTAKIDNISYAIDE
                     IGFSIVGVFQSKH"
BASE COUNT         6213 a         4341 c         4050 g         6608 t
ORIGIN      
        1 acatcatttt cctcaaaatt gtgtaaacag ttgtaatcat gcaatataac attggtatca
       61 atgacaaaat tcttttttga actcatggtt ggtattaatt gaagggctga atttatttca
      121 gatatctaat tcgcctaaaa aggctgaaaa gttctcactg taaatatcgt gagttatttt
      181 ctattttcaa acaaaaaaca tgaagaatat aatcgcattc acgcagactg agattttata
      241 gatctaaaag tgccattatg tcatgaaaaa taaaaaaacc gacaatttgt cattatcggt
      301 tttttaataa ggaaaaataa tattcttttt tattcgctta aagcattatt ttttgggtaa
      361 acttaccggc tcgaactata taaatgcctt tattcaggtt gacgagtgat atgcttgttc
      421 cgttgtacac ggtagtgagg actttggctc ccagcgcact gtaaatatct atctgcacgc
      481 cattatccat attgctcaca ttcaacgtct tacctgtaac agtaaatttt ggaactacat
      541 cgctttccga tgtttttgta ctttgtgtat tgcttgtttc agtggtgttc ttgtcaaaaa
      601 gagtcgaatt tttttgagat tgaattcccg ctattccaca aagtataaat gtcagtataa
      661 gagtaaattt tctcataggc ttgatttttt ttaatacttt gtaatcatca ctacaaagat
      721 aatgcaaaac tatagaaact gtatctttat tttataaaaa aataaatttt aatgattctt
      781 tcaggctgag agtgtttaat ttatacgaat aaagccacct ttccggaggc agcaaaatat
      841 tatattaatg gttattaaaa actgaattac aactatataa ttccttcatt tatcattaaa
      901 ttttcactat ctttgcacga ttattttaaa atttgagatt accgatgaat aagattgaac
      961 cggagtatat ttttgagaca agttgggaag tttgtaatat ggtcggcgga atttatactg
     1021 tactttccac gcgatccgaa acactataca atcattttaa ggataagttg atttttatcg
     1081 gacccgatat ctggaaagaa actgaatcac cctactttga cccatccgaa gaattgctca
     1141 gcgaatggcg ggaacacttc acccgcacca cgggactgcc cgtacgtacc ggccgatgga
     1201 aagttcccgg aaatcccgtg actattctgg ttgatttcac tccgctcaca taccgtaaaa
     1261 atgaaattta tggccatatc tgggaaaaat acggagttga ttcgcttcac ggctacggcg
     1321 attacgacga atcgtccatg tttgggtatg ccaccggttt ggtgatcgaa agttattacc
     1381 aataccacaa tctatcggcc gaaaataatg tggtagctca tttcaatgaa tggatgacca
     1441 ccttcggagc atttcacata caggaagctg tacccgaaat cgcaactctt tttaccacac
     1501 acgctacctc cattggccgt tcgatagcag gcaaccacaa accgctgtac gattatctga
     1561 ccgaatacaa cggtgatcaa atggcttatg agttaaatat ggtatcaaaa cattcaaccg
     1621 aaaaaaaagc agcacatatt gtcgattgtt ttaccaccgt gagtgatatc accgcccgtg
     1681 aatgcacaca actgctggat aagacaccgc atatagttac tcccaatgga tttgatgaca
     1741 ccatcgtacc cacaggaaaa ttatttgatg acaaacgaaa aaccgcccgc gccaaactga
     1801 aagaagtaac agaaactctc attggttaca aactcaacga tgatgcatta ttcatcagta
     1861 ctgcgggaag atacgaatac aaaaacaagg gtatcgatac attcatcgaa tcgctcaaaa
     1921 aactaaccaa tcacaaggaa cttaaccgcg aagtggtggc tttcatcatg gtacccgccc
     1981 atatcaaagg tccccgggtg gatcttaccg caaagctcaa tggctcagat attgctattg
     2041 attactggaa caaacacacc actcacgaac tgtatgacta tcaatacgat aatgttatct
     2101 cggctctgcg ctggtttcac ttcaccaatc tgcaaaacga gaaagtgaaa gtgattttcg
     2161 ttccgtcata tttgaacgga aacgacggca ttttcaatat gggatatctc gatttgctta
     2221 ttggtatgga tgtgagcatt tttccctcat actacgaacc ctggggatac accccacttg
     2281 aaagcgtggc gttctcagtg ccaaccatca catccgattt atcgggattt ggtcaatggg
     2341 taaaatcaca ctacgacaca agacacggtg tggcagtggt tcatcgctcc gactacaatc
     2401 aggcacagat aagtgaagaa attgggaata tactggcaca atttgccaat tactcagctg
     2461 cggaagcaga aaatgtcagg aaacaagcac tggagacatc caaacaagct ttgtggaacg
     2521 agtttattca atattattat caggcttacg atattgcgct gaccaaccgt aataaaagaa
     2581 taaaacactg aattgaagct caataccaat ttctgcatac gattgaaatt ttttattatc
     2641 acatttaact atacacctct tcaattttaa ggatattttc tctaattttg cggatatata
     2701 tgctataaaa catattttta gagcataata aataatgttt tcgattaaaa acaagaataa
     2761 atagcaacta ttttttatga aacttaaagc aaacaataca aatgatgtac cctggaggaa
     2821 tatcattata aagtcaaatc ttccggaaaa tctgaacaaa ctggatgaaa tggctcaaaa
     2881 catttggtgg tcctgggata gtgaagcaaa gaaattgttt cgtgaaatag accgaccggt
     2941 gtggaaagaa gcagaatcaa gcccggtaca attgctcaat cttctaagct atgataaatt
     3001 ggacgctttg gctaataatg aagaattcat agccaaggtg gataacgttt acaataaatt
     3061 caaggcttac ctgaacgagc ccatgaatac aagtcgccct tctgtggctt atttcagtat
     3121 ggaatatggc ctgaatgaag tacttaaaat ttattcaggc ggacttgggg tacttgccgg
     3181 cgactacctc aaagaagcaa gcgacagcaa tgtggacctg accgctgttg gttttttgta
     3241 tcgatatgga tatttcactc aatccctttc ggtggatggt gagcagattt cagtttatga
     3301 accgcagaat ttcaacacat tgcctatcaa acaggtgatg gatgagaata acctaccgct
     3361 ggaagtggaa gttccatatc cgggacgtac ggtatatgct catgtatgga aagtatcggt
     3421 aggacgtgta gcgctttatt tgctcgatac agattttgat aaaaacaatg attacgaccg
     3481 ctccattaca caccaattgt atggcggtga ttgggaaaac cggctgaaac aggaaatcat
     3541 gctgggtatt ggcggtattc tgaccctgaa aaaactgggt atcaaaaaac agatttacca
     3601 ttgcaacgag ggtcatgcag cacttatcaa tgtacaacgt ctggttgact taattcagga
     3661 cgaacatctt actttcaacg aggcaattga agtggttcgt gccagttctc tctatacggt
     3721 tcacacccct gtacctgccg gtcacgacag cttcgacgaa ggcttgtttg gcagatacat
     3781 gagcgaatat cctgcaaaat tacaaatttc atggggtgat tttatggata tgggacgcga
     3841 aaatcctggc gacaccaacg agaaattcaa catgagcgta tttgcctgca ataccaacca
     3901 ggaagtaaac ggtgtgagct ggcttcacgg cgaagtgtca aaaaaaatgt ttgctcccat
     3961 ctggaaaggt tatttccccg aggaatcaca tgtaaactac gtcaccaacg gcgttcatct
     4021 gccatcttgg gcttcatcgg aatggaaggc actctacgaa aaatacttta ccaaagcttt
     4081 ttacgaagat cagtccaatc tgaaaatttg ggaaaaaatt catcaggtac ctgatcagga
     4141 aatatggaat ctgcggatgt cgcttaaaaa caaattggtg gaatatatcc gtaatcagtt
     4201 tcaggaaagc tggttgaaaa atcagggtga cccgtcgcga atcgtctcga tactggattc
     4261 aatcaatccc aatgccctga taatcggctt tggccggcgt tttgccactt acaaacgggc
     4321 acatttgctt ttcaccgacc ttgaaagact ggaaaaaatc gtaaatgatc ccattcatcc
     4381 cgtacagttt ctctttaccg gcaaagcaca ccccgccgat ggagcaggca aaggccttat
     4441 caaacgtatt gtggaaattt cacacatgcc gcagttcctg ggtaaagtga ttttcctcga
     4501 aaactatgat atgcgtcttg ccaaacgtct gatttcgggg gtggatatct ggctcaacac
     4561 acctacccga ccactggaag cttccggtac ttcgggcgaa aaagctcaga tgaatggcgt
     4621 actgaatttt tcggtactcg atggatggtg gtacgaagga tacaggaaaa acgcaggttg
     4681 ggcgctgacc gaaaaacgga ccttccaaaa tcaggaattt caggacaaac tggatgccat
     4741 caccatctac aatatacttg aaaatgagat aattcccctg tattttgcta aaaacaccaa
     4801 aggatattct ccggaatgga ttcaatacat taaaaattca atcgctcaga ttactccgtg
     4861 ttttaccaca aaacgtatga tggatgatta ctttgaaagg ttctacaaca aactggcaaa
     4921 aagaggtggt ttattgaagg ccaacgattt caaagtggcc aaagaactgg ctgcctggaa
     4981 agaaacattg tctgaaggat ggaataacat cgaagtagta agtgtaagca ctactgaaaa
     5041 attcattacc aatccgcagg tgggtgaaaa ctacgaagca aatgtagtta tcgataccaa
     5101 aatgaacaat gagaaaaaca ttggcattga aatggtaaca atttctaccg atagaaacaa
     5161 tgttgaccac ttcttcaaag cctccgagat gaatctggta aaaacggaag gaagcaaaat
     5221 gtacttcaac ctgactttca agcttcatca ggctggtgtg tacaaaacca gttatcgcat
     5281 gtttccaaag aatgaattat taccccatcg tcaggatttt gccactgttc gttggtttta
     5341 ataatgcctc attacaacta aaaaaacctg taaagaaaat tcttcacagg tttttttttg
     5401 aacaccctca gctgggtggt tttcgtttta gaaatgaaac atgtaataat tgtaatgtaa
     5461 gctttctaat cccatttcag aataacttta ccgcattgtc cgctttccat tacatcgaat
     5521 ccctgctgga aatcgtcgat agagaaccgg tgtgtaatca cgggagaaag atccagaccc
     5581 gtgagcaaca tctgttgcat tttataccag gtttcccaca tttcacggcc ataaataccc
     5641 ttgagtgtca atgccttgaa aataatttca ttccagtcaa cagtagtaga attgggtaaa
     5701 atcccaagca aagctatttt tgaaccgttg tacatattat caatcatttc gcggaatgct
     5761 actggtgccc ccgacatttc caaaccgaca tcaaacccgc gaagtttaag ttctttgcgg
     5821 gctccttcta cagtttcacc attttttcca tttacagtat atgttgcacc cattttacgg
     5881 gcaatttcaa gtcggtattc gctcaggtcg gttaccacga tattacgcgc accggcaaat
     5941 ttacatatcg cagttgccat ggttccaatc aggccggcgc cggtgataag tacatcttcg
     6001 cccagcacag ggaatgacaa cgccgtatgg gtggcattac cgaacggatc catgatagag
     6061 gctatttcgt caggaatacg gtcatcgaga tgcaccacat tcacttcggg caacgaaaga
     6121 tattcggcaa aagcaccatc gcgatttact ccaattccga cagtgttttc acacacgtgt
     6181 agttttcccc tgcggcagtt acggcagtgt ccacaagcta tgtggccttc accggtaaca
     6241 cggtcgccta ttttcacgtt tttcacacct gcacctttat ccactaccac acccatgtat
     6301 tcgtgaccaa tggtcatggg ggtctttatg gttttttgtg accagtcgtc ccatttataa
     6361 atatggagat ctgtaccgca tatagctgtt ttttttattt ttatcagcac gtcattaata
     6421 cctatttcag gaaccggaac attatccatc cagataccct tttcaggccg tagttttact
     6481 aatgctttca tatttagtta tttagttatt ttattattgg gttattgtct ccattagata
     6541 aggagagttt ttctgttatt gagatttcga aaaagaggct cagttcttta tttaattaca
     6601 ccaaattctt ttgctacttt agtaaatgca gcgatgcatg tatccaggtg ttggggttca
     6661 tgagcggcag atatctgtac ccgtatacgc gcctgtcctt tgggcactac cggatagtag
     6721 aaaccggtta cgtagatgcc ttcatcgagc aggcgtgtag ctatatcctg cgagagtttg
     6781 gcatcataaa gcattacggc acaaatggca gattctgtcg gtttgatatc aaaaccggca
     6841 tctttcattc gtttcacaaa atattcggta ttggcgtgca gcttgtcctg cagatcgttg
     6901 gtagcatcta tcatctcaaa catcttgata ccggcagctg ccaccatcgg tggaatggaa
     6961 tttgaaaaca aatagggacg tgaacgctga cgaagcaatt cgataatttc tttttttccg
     7021 gttgtaaatc ccccgattgc accaccaaag gcttttccga gggttccggt gatgatttca
     7081 atttttcccc gtaagtcata ccgttcggta acaccgcgtc cggttcggcc aactacaccg
     7141 gctgagtgcg attcgtcaat catgaccatg gcattgtatt tttcggccag ttcatagatt
     7201 ttatcaagcg gagcgacatt gccatccata gagaaaaccc catctgtcac aatgaggcgg
     7261 tagcgttgag cctgagactt tttcagttgt tcttccaggt cgtccatatc agcattggca
     7321 tagcgatagc gctgtgcttt gcacagacgt acaccgtcaa taatagaggc gtgatttaaa
     7381 gcgtcggaaa tgatggcgtc ctcctcgttg agcaggggct caaatacacc accgttggca
     7441 tcgaagcagg cagcataaag aatggcatct tctgttccaa aaaatcggga gatggatgct
     7501 tccagttgtt tgtgcaaatc ctgagttccg cagataaaac gaacggaaga aacaccgtag
     7561 ccgtgagagt caatggcttc ttttgcggcg tctttaagag tttggctatt ggcgaggcct
     7621 aaataattgt tagcacagaa gttaagtact tcttttccat cggctacacg gattactgct
     7681 ccctgaggtg aaacgatgat tcgttcattt ttgtacagac ctgcttccca gatattggta
     7741 agttcagttt gtaaatgtga tttaaattcc tgatacatag taattttatt tataaggtgt
     7801 ttcttgtttt acaacgacca caaaggtatg gattctatgg aaaaaaaaac gtttatttcg
     7861 ggataaataa ccgaatgaaa ttagcatctc cgataaaaaa aatgagagta ctactccttg
     7921 ccgggtagta tcagtgattt aacatcaaca acctgaattt tttcataacc catttctgtc
     7981 aatatcgaag taattatcaa atgtgcctgt ctggaagcat cgtccagtat cccctgactt
     8041 acagcacttt tggttatcgt taatttttct ttcgatatgg ctttgttata atcattgatg
     8101 gagatgggat tgaaagcatt catgctttca tcatataccc ggatggattt ctcatcgatt
     8161 acagtttcga aaatttgcgc ttttggtagt tgaacgacca cactttttcc atccactttc
     8221 acggttgctc ctttcaaatt gacaccagct ttgatatacc cgttgtattt caataaaaaa
     8281 aacttttcgg tgagtggcac ctgaaaattc attatggtca tataatcctt gaaacttatc
     8341 actccggcat agttgtacct gacaagcgtc agttcctgta caaatacaat tttattcaat
     8401 accgtagtac tgtcgtaaat cgctttccgt tcggcttttt tagtctgaat aagcatgact
     8461 gccaaaacca ccgtggtgat aaaaaaaatc agtgctgagt aatttgttct tttcattttc
     8521 caatagatat tattgaaaac tcataattaa aagcatttat ttgccggtaa aaaaataaag
     8581 taaggtgatt aataatccga ccgatgccac aactgccaat aatattttaa tcatcaattt
     8641 gacaaattcc ttgttttcca ttccattttc attcatattg atcggggcta aatgaaggtg
     8701 agtttttttt acttcaacaa agataattga ttttcttaaa ttcagatttt caatctgtaa
     8761 agttttctta agctttcggc aagttagtcg ggaaatagct cccaatcttc tgaaaagttc
     8821 ttaaataacc acaattttaa agttaaaaca gattgttaat cagcatgtta actttattgt
     8881 tcgtaacttt gcacaattat tccgaattag tacgttttta aatgcgtact gatattattc
     8941 actcattctg tattctatga tttttccatt caaaaaaatt cttgcaattt tagttatcat
     9001 tgccatcacc attccctgca tttcaggatc aaaaaaacca tcaaaaacta ccgaaagcga
     9061 accaattcca attacggtat tattgcaacc aaccattgaa tcggtgtatg acagtctgca
     9121 tcttgatcag aaaggacttt ccaaagtggc ttttgattat gccataaatg gttacaaaga
     9181 actgaaacaa aaaggaatgc ttcacaatga ttccattatt accatcgttg attttgacca
     9241 accaagctac aacaagcgca tgtacattat tgatgtaaaa aatttcaaaa tgctattcaa
     9301 tacctggact gcacacggcc gtaataccgg ccaggaaaca gctaaatact tcagtaacag
     9361 acctcaatcc tatcagagca gtcttggatt ttatcttacc gatgcatcat acaacggtaa
     9421 aaacggatac tcgctaaaac tcatagggtt ggaaaaagag aacagcaacg ctttgaaccg
     9481 ggcaattgta ctgcatggtg ctccttatgt gagccagcgg atgataaacg ctcagggata
     9541 tatcggccgc agtcagggct gtccggcagt acctcaggag ctcaataagc caatcataga
     9601 gaaaataaaa ggaggatcgt gtttctttat ctataataat gcgtatcatt tcgacaaccg
     9661 aatataaaac cagttgatat ttctgaaaaa gccggataaa aaatccggtt ttttcgtttt
     9721 aagtactttc aattccggac tgattctaaa attatatttt gtaattttgt aaaaactaca
     9781 ttttcgtttc tcttccttca ggatactaaa aaataaagat aaaaatggct ccacttgctg
     9841 aacgcatgcg tccgcagacg ctcgaccaat atatcggaca aaaacatctg gtgggtaata
     9901 atgccatttt gcggtcgatg atagactccg gtcatatcgc ctctttcata ctctggggac
     9961 ctccgggtgt tggcaaaact accctggcca ggataatagc caatcagctc gaaaggccgt
    10021 tttacacgct gagcgctgtt tcgtcgggcg tcaaggatgt gcgcgaagtg attgagaaag
    10081 ctaaatcaag tcgtttcttc tctcaggcta atcctattct ttttattgat gaaattcacc
    10141 ggttcagcaa atcgcaacag gattcgctgc tgggagcagt tgaaaacggg acagtgactc
    10201 tcattggcgc caccaccgag aacccttcat tcgaagtaat tactccgctg ctttcacgct
    10261 gtcaggtata tgtgcttaaa cctctggacg ataacgatct actggaactg gccgatcggg
    10321 tgataaaaaa cgatgtggaa ttaaagaaaa gaaatatctt tattgacgaa aaagaagcca
    10381 tgcttcggtt ttcgggtggc gatgcccgta aactgctgaa tataattgaa ttagtagtca
    10441 actcggaatc ggatccggaa gttcacataa ccaatgagac cgtaaccaat cgccttcaac
    10501 aaaaccccat ggcgtatgat aaagacggtg aggtacacta cgacattatt tcggctttta
    10561 ttaaatccat acgcggcagc gatcccgatg gtgctattta ctggctggca cggatggtag
    10621 ccggcggcga ggatccgaag tttattgccc gccgtctggt gatatcggct gcagaagata
    10681 tcggactggc caatccgaat gcattgctgc tggccaatac ctgttttgat gccttgcata
    10741 aaatcggctg gcccgaaggc cgcatcattc tggctgaaac taccgtttac ctggcgtcat
    10801 cgcctaaaag caattcggcc tatctggcta tagacgaagc gctggctttg gtggaacaaa
    10861 cgggcaatct gcccgtacca ttgcatttgc gcaatgctcc taccaaactg atgaaaaatc
    10921 tggattacgg caagggatat aaatatgccc acgattttga aaataatttt gtcaatcaac
    10981 agtttctacc cgataaaata aaggacaagc gcatctggaa agtgcaggaa aatgctgcag
    11041 aaaagaaaat gaatgaatgg ctccggtttt tatggaaagg caaatattaa tctggcatga
    11101 agattgatat atctgagcta taatcactaa actttgttat tatcatgaag aaaatttcta
    11161 ttctccttct atcattactg tcattgatgg gttttgcaca aaaatcagcc taccaggtca
    11221 ccagtcctgt ggagttgaat gtaatgactt tcaacatacg gttggatagc gaaaaggatg
    11281 gcttaaacaa ctggaaaaac cgaaaagaca ttgtagccaa tactatcaga tactatgaag
    11341 cagacataat aggaactcaa gaagttcttc acaatcagct gctggatatg gaggcgttgc
    11401 tgcccggcta ttcctccatc ggtgtaggac ggctggatgg aatggaaaaa ggtgaataca
    11461 gcgccatttt ttataatcac aataaactga cagaaaccgg atcgggatat ttctggctca
    11521 gcgaaaaccc acttgcagtg ggtaataaag gctgggatgc agcctgcgaa cgcatagcca
    11581 cctgggccaa actgaaagac aacagaaccg gaaaaatttt ctttgtactg aatacgcatt
    11641 tcgaccatgt aggtaaaaaa gcccgaacag aaagtgccaa actgatactt gaaaaagtaa
    11701 aactttatgc gaaaaaactg cccgtcatcg taacaggaga ttttaattcg acacccgaat
    11761 cggaaacaat caagattctg accgatacat caaatccatt acatctcacc gactcacacg
    11821 cggtatcacc tactgtcgaa ggtcccgact ggacattcca tgatttcgga cgcctacctc
    11881 tgaaagaaag ggaactgata gactacgttt ttgtgaaaaa caatgtaaac atactgacct
    11941 acaaagtgat agatgaaaaa gtagatgaca agtatacatc cgatcacaat ccggtactgg
    12001 tgcgggttca gttttaacaa agactagata ctatacaaaa acaggcttaa ctctaagaag
    12061 aattaagcct gttctttgtt taattacaat atcaattttc atctgtatcg gtttccagcc
    12121 agaaattttc accatcaaaa attccgtagg agaaaatccc gacaaaatcg cccagaataa
    12181 ttatccggct ttggttttgg agctgcaaat ccagttcaat atgccggtga ccaaaaatca
    12241 ggaaatcaac ctcatgagat ttcacataat ttttagcaaa aattacaagt gtttcatttt
    12301 cttctccctg atacttattc tcaatttcct gaattttcaa tcggttgttt ttagaccagt
    12361 tatagccaaa attttgtcct atatcgggtg gtatccactg aaacattttc tgacagattt
    12421 tacagtgaaa tatacttcgg atgagtttaa atcctttgtc atcgacaaaa aggccatcgc
    12481 catgtgcgag aaagaatttt ttcccatgga tatttattgt ttcattaccg cgatgtacaa
    12541 tcaacccgat ttcacgctcg agatacccga atgtccaaac atcatgattg ccggtgaaat
    12601 aatgaatttc gatgccgcta tcggtaagtt cggccatttt tcccagaaaa cgggcaaatc
    12661 cccggggaac aacggattta tattcgaacc agaaatcgaa catatcgccc agcatgtaaa
    12721 tagcttcggc atcttttttt accctgtcga gccagtttac aagccgcatt tcgtgctttc
    12781 tgggatcctt taccaacctg gaaccaaggt gtgcatctgc aataaaatag atcatgtagt
    12841 atgtgttttt acaataaaga cgcattattt tgcgtctcta tcgatgaatt ataaaaagcc
    12901 cagttcaagt tttgcttcct ctgacatcat atctttgtcc cagggtggat caaatacgat
    12961 gttaattcgg gcttcgctga taccgggtat gctttctacc ttcatgcgca catcttccac
    13021 gataaaatct actgcaggac aattgggggc tgtgagggtc atatcaatgt ccacgctgtt
    13081 atcttcctgg ggttcaatgc ggtaaataag gcctaaatca tagatattta cgggaatttc
    13141 aggatcaaaa actgttttga gcatcctgac tattttttct tctctctcta aaaattcatt
    13201 cataatctga catttgttta acaaacaatt taattgcaat aatgttgacc tctcctatga
    13261 atttcaggca ttagtttgtt ctaccaatcg gtcaaataat tcgaagtcgg ctgtcagttt
    13321 tccatcaatt gtaaccactg tagccacttt ggcagttaca cacagttgat tgtcgggttt
    13381 ccggtaaata gtttgatgaa aaacatactt tacaccctct ttcactacat acaatttgca
    13441 cacaaactta tcgcctgagc gaagcggata cttgtaggct atctcaatcc ttgccaccac
    13501 tgcatcaatg ccctgatcat gcagatctga aaaactcacc tgatgattaa gcaaaaattc
    13561 gtgtcgggta tgttcaagat aattctgata gacagcattg ttcacaatgc cctgtaaatc
    13621 acattcataa tcgcgaacct taaattccag ttcgtattta tacttctcga tagtcatttt
    13681 atagatattt actttttacg aattaaagtg agaccgtcgc gcagggggag aatgactttc
    13741 tccacccggt tatcagtggc tattagttca ttgaaccgta aaattccaag agtttgctga
    13801 tcgttttgat gtactttttc caccactttg cctgaccaaa gtgtgttatc ggccagaata
    13861 aatccaccct gtctgatttt gggcaataca gcttcatagt aggcttcata ttcgcgtttg
    13921 tcggcatcaa taaaaaccag atcgtatgtt ttatcgagtg tcgggattat gtccaaagcg
    13981 ttaccaatca gcaaattaat tttatcttca aagggagttt tttcaaaatt tcgtaaaata
    14041 aattcttcaa gctcatcatc gctttcaatg gtatcgagtt caccgttttc gggcagcgat
    14101 tcagctatgc acaaggccga ataaccggtg aaagttccaa gctccagtgc atggaccggt
    14161 tgtatcattt tacagagcat actcagcacc cttccctgca aatgtccgga gcacatgatg
    14221 ggattaagca tacgaatcca tgtttccctg ttgagccggg tgagatattc aggttcgggg
    14281 tctatgtgct gcaatatgta atcgtcaata ttcatcagtt atatttcaat aatgtcagat
    14341 tttgaggatt gaaaatttca atcgctactc gctgtaattc ttcagaggtg atggtattaa
    14401 ttttcttacg tacttcgtcc atcccatcaa acttcccaaa acgtaagtaa cttttaccga
    14461 gcgacaatgc cagattttct ttgttctccg acgaaatggc tatttgtccc atcaattgca
    14521 atttatactt attaagttta tgttctttaa tagggtttag gcaaagcgaa gccagttcct
    14581 gacgcaccaa attttcgcat ttgtccgcat tttcgggatc acagccgtag tataccgccc
    14641 accagccaca atcagtaaac gcctgatatt ctgcttccac attataaacc aatccatgtt
    14701 tttcgcgcag cgataagttc agcaggctat tcatgcccgg tccacccagg atattattca
    14761 acagataaag tatcatcctg tcggggtggt tgatatcata agcccgtgtt ccgatcatac
    14821 agtgaatctg gtgagtgttt cgcttaacta ctttttgttc agccggctgt accggtggag
    14881 caattcgggt atttactttt ctgttacgct ctgattcaat aaaatatttc tggcaccatc
    14941 gtacaatttt atcaaaatcg actgctccca atgaaaagaa taccatttcg gtgggcaaat
    15001 agtgactgtg atagaattca agcgctctgg cagtgtcgaa gtgttcaata tttttcttat
    15061 ttcccaatac attgtgcgcc atgggattgg gcttaaatag taattcttca aaatcatcga
    15121 aaatcaattc ggaagggctg tcgttgtacg attgaatttc atcctgaatg atttcgattt
    15181 ctttgtcaat ttcgttttgc ggaaatacag aattttgaac aatatcggct attaattcga
    15241 tggatctttc ggtatattca ttcaggaaac cagcgtaaac cactgtttct tctttagatg
    15301 tataggcatt aagctcgcct cccacatctt caaggcggtt gattatttga cgcgatgttc
    15361 gtttttctgt tcctttgaaa agcatatgct caataaaatg agccattcca tactcacctt
    15421 ttttttcttc gcgtgaaccg gcattaataa ttacaccaca gtatgaaaca agtgaatttc
    15481 cgggtaaatg caccaaccga agaccatttg ataatgtatg ggttttataa ctcttcaaaa
    15541 cttaatattt tatggagtgc aaagttaaca atttttactg aagcaaaaaa ggctaccagt
    15601 caggcagcct tttttgctaa taacttataa ttaaactatt ttcctttctt caggcttgaa
    15661 tcaatgcttt ttatcacctg cagtgatacg gcatccgttg gatcgatttc aagcacttta
    15721 ttccaaaatg ttttggactg taccaattca ttttttacat aataataata tccaaggtaa
    15781 cgataacatt ccagaatttc ggctttacgt tcattttgag atggaagcat tacttccagt
    15841 gctttttcgt aatatggctt agcaagtcct tcggttgttt cggggtcaag agcagaattc
    15901 gttcttgcac ttgacagata gcccagataa tggtccggga attttacgct catagatttg
    15961 aacgtgctgt cggctttcaa cagatatttt tttcgggctg ctttgtcctt ggtagccacg
    16021 gtatccagtc cggcattgta gtatgcgcca cccagtgtgt atatgtcagc catggaatga
    16081 cctggatttg agctaattgc atgttgataa ttttcaattg ctttgggata atttttagct
    16141 ctttcatatg ctttagccgc ttcgctcaca tattgtagtt tggtagtatc cagtttgaat
    16201 gcagcttcat aatttgcagc agcgtcgttg tattttttcg ccttggcaag aactttagca
    16261 tacgtggtat aatccagtga aataacatcc ttgacaggtg tttctttcat aaatttagct
    16321 ccaattgctt cggcttcgct caaattacca agttcattta aactgtacat tttcagacgg
    16381 tccattacat aatcctgtgg cgctttgcta atagcttcga gtgcttttga gtactgctta
    16441 ttgaagtata gaatggtagc atactgcgca taatctttgg gtgtaggctt tactaatcca
    16501 aggtattttt cgtacgcctc catcgcttga gggtaaaatc ccttacgata gctcatttct
    16561 cctaaattgg aataagcaat ggcatactgt gggtcaacgt caatagcctt ccgaagatat
    16621 tcgaaagcca gttgagttct ggtagtttcg taaagattag ccagtttcaa caatgctgct
    16681 tttgaatttg gatcaaaata gattgcattt tcatatctca cagcagcttc tccgttattt
    16741 ttttgggaaa ggaacttatc accttcaata atataaggaa gcgcactttt cttattggct
    16801 tttttggcct tggccagaaa agcttttgct tgtaaatcat tattattgag ttggtaagcc
    16861 tcagctattg ctgtaaaaat accagcatct tttttgtatt gtttgagttt cgaaactgct
    16921 ttcagcatat tatcggcacc tttaagatcg gttgccattt taaccgttgc caatcccacc
    16981 gtattataag aatttgcagg gtcaatctgt aatcctttct caaaataata ctttgccgaa
    17041 tccattttgt tttgtttgaa ataaatctga cccaggtagt aactgtccat cgcatccaca
    17101 ttgccagcaa ggaagtgtgt ttttgcagtt gcataatcac cagcttcgta ataatctgtt
    17161 gccatcttgt tgcctgcaga aacaaaagtt accacgccta caagtaaggc taaaatcata
    17221 gcttttttca ttttctcaat catttaattg tttatattta gttattatat tattctgttc
    17281 aataattatg cctaaaaatt ttacatatta gttcagtcct tgatatgtac ggtacttatc
    17341 ggcatagtgg atggcaataa tcctgaacgc ttgattattc gttgaccctt atcgctcgcc
    17401 acgaagcgtg aaaatcctct gggtagttcg ccacgtggat cattaactat aaagtaaatg
    17461 tcgcgaacaa acgggtattg gtgtgtgtaa atatagtact ggtagggttg tacgctattg
    17521 tatctgtcag ctttatcttc tcttgacaca cgcatgacct gaattttttt tgtaaaatca
    17581 atagctgtag tgtcggcatc gtcgcttatg agattaacac cgatgaagcc gagtgctgcc
    17641 ggtgattttg agacataatc caccacttct tcattaagtt ccaaagcgct tagattttga
    17701 gaaattggtt tatccctgca tattgaatca atagcgtacc gcaccgtact tgattttgtg
    17761 ttgtcaaata ctacctgaat ttttcctaat tttgatcccg ggtaaatttc actccatttg
    17821 gtgtattcac cggttaatat tttttttacg ttttctacat taatgatgga atccggattt
    17881 tgaggatgaa taataagtgc tatcccatca atggctatac gtacattttt tggctggaat
    17941 tttttttcct cgaaaaaaat catttcacct tcggtaagtt ttcgggcagt tactattaat
    18001 ctgacgctgt cctttttgag tagttcaatt gcttcagttt cggtggtata tacaggttct
    18061 attttggcgg gatatagtcc ttcgaacaca tctatttcat tctgtatcac tttttgcatt
    18121 gtctgatcta ccgctatttt gatataaccg ctggtagtag tgttttcatt tttcacttct
    18181 ttttttcctc cacacgaaga gaggaaagct atgcttagca ataaaacgat taaaatatga
    18241 aaatttttca tacaattatt ttttgaaaga aaatggttat aaaatgtaag agattgacct
    18301 gtactactca aatagattat ttaatttttt gtttaatatg ttttcaaact tattcatcaa
    18361 tcattaatga aacaaataat acatataact tgtttcagaa aaataaaaat tttactcaca
    18421 tgatatgctt attattcagt ggtttaggtt agacttaaca tattgaattg tgaaagatta
    18481 aatacaaaca cacaatatgt aacaaaaaca aaatttaatc tgaacctgta aaaaaacaag
    18541 ccggattaaa ttctgttttt tgttttaagg ttatactgtt actgtagtct aaatgaaact
    18601 ggaagatagt attttacctt tacggcgtta ccaccttgtc ttccgggtat ccatttaggc
    18661 attgatttga ttactcttac agcttccttg tcgagacttg cgtctacact gcgttcaaca
    18721 cgaacatcct ggatagagcc atctttacca accacgaatg agcaggttac ttttccctga
    18781 atactatttt cctgtgcaat agccggataa ctgatattgt tattcaggta ttccatcagc
    18841 gcagcatcac ctccggggaa cgatggtggt tgttccacca cttcgaacac cttttcgggt
    18901 tcttcttctg ccacgattac tttatgttcc tgaagtgtag caatatccac acctgtttct
    18961 tcgttggtac cttttacgtc ggccaccgaa atcgctactt ttgattccgt tatttcttcc
    19021 tgagatttta cctcattttc ttctttcact tcctcatctt ttttgattat cggggcagta
    19081 aatttcatac tacttttcaa ctctggtggt ggaggcgctt tgaattgttt ctcaatattt
    19141 tcttttggca cttctgcctt tttcatattg atattcgaca tctgagctac ttcagtcatc
    19201 aattcctttt tgcggttctg attatacata tttatcaggc tcgctgaaag aaaagtaagt
    19261 acagctactg ttcctactcc gagaaatgct accaaatgtc gtttggggga acttttacgc
    19321 agcgtatatg ctccgtaggc tttatttttt ccctcaaaaa tcaaatcgat ccagtcctga
    19381 gaagttaaat taacatcttt tgccattttt caattattaa atgataatta attaattctt
    19441 tggagcttct tcatactgaa ctttggaatt tttcagtagc gttttatcgt tagcatccaa
    19501 ttcggtaatt gcatatttac ctacattaca tataagcatt tcgtcaagtg ctgaaatcat
    19561 atcgttgtac gtggagttat caagtggttt gataactact accggtgtcc cgggactact
    19621 tttcagtgtt gacaattgct tttcataatc tgcctgtgta acttgtaact ttaacttttt
    19681 atctttaagt tcgttaacct ttgctacgat tgcagcattt ttctgaataa ggatttttct
    19741 taatccttcg ggtgaaaagt cggtttgctt taaaaagtct ggtttatcaa gttcaggaat
    19801 acccataaaa tagtacacct tgtgatcctt ggataaatag atagtaacag cctccgaagc
    19861 ttttgcttcg ttcttgtcct tatcttcaac tttatctttg gtaggcatac cgatttccat
    19921 tgtctgcggt ttgctcatac tcgtggcaag cataaagaaa gtaagtaaca acataatcat
    19981 atccaccatg ggggtgaagt cgatacgtgt actcaatttc ttcatgtggc cggctttctt
    20041 tttaccgccg ctatctttct gttgaatttc tgctgacata ttcttaattt tttacttcaa
    20101 aacttatttc aatttaaata aaataaagtt tattgatttt attaatctgg ttttggtgca
    20161 ctaacttcac gcaatgaagt tataagattg tacctgttct ctcttaagtc ctgaagagag
    20221 cccattacgg ccttaatctt cttgtaagga gtgccctggt ctgcttttat agcaataacc
    20281 atgtcttttc cttttacttc gcgtgccgct ttcacccaag cctgaaattg attgtcagta
    20341 ctgtcggttg gaacaccata attagccagt tcaacttttt gtttttctcc gggtatggcc
    20401 atgaattcct tgaattgctg aataggcgta ccaaaggatg tagcaatact aaaatctttc
    20461 ttttcctttt cagtcagatt gatttgatat tcagcagcaa cccgttccag tatagcaatt
    20521 cggtcatcgg gattatcaac atccagaaaa actttcccat caggagaaat taatacagag
    20581 aagatatttt tttcgggtat ctttatttcc gaaacagatc cgggggtatt caccttaacc
    20641 ggctcaggct tcaggaaagt agaggtcaac atgaagaagg tcaacagcaa gatcattacg
    20701 tcgctcatcg gggtcatgtc gatccaagta tcttttcttg gtaattttat tttcggcatt
    20761 gttcttcgtt ttattaatta attgaatcta atttaatatc ggtaattaag ctgctgataa
    20821 aaacagtgcc taattactac gcttcgttga atccgattca aatattaatg tttcgattgg
    20881 aaaacaccta ctattgaaaa tccgatttcg tcgattgcgt aagagatatt gtcaattttt
    20941 gcggtgaagt agttgtaaga aattacagcc aaagcaccgg tcaagatacc gaaagccgta
    21001 tttacaagag cttcagaaat acctacagaa agagcagcag agtcagttcc accttcgcct
    21061 gccaaagcag agaatgaacg gatcataccg agtactgtac caaacaaacc ggtcagtgta
    21121 ccgagtgctg taaatgtagc aattacagga aggttttggt tcatagtcgg catttccagt
    21181 gctgtagctt cttcgattgt tttgttgata gt
//
LOCUS       DAOPZK010000019        20546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2680_length_20546_cov_25.005759, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000019 DAOPZK010000000
VERSION     DAOPZK010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20546
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2680_length_20546_cov_25.005759"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /gene="dprA"
                     /locus_tag="PKH68_02195"
     CDS             complement(<1..1100)
                     /gene="dprA"
                     /locus_tag="PKH68_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNX11776.1"
                     /translation="MEKDELLKYQIGITLIKGIGPNLAKNLIAYVGSVEGVFREKRQN
                     LAKIPGIGEVLSREIVSQDVQARAEKEVNFVVKNNILTMFYTEKSYPYRLKECADAPA
                     MLYYKGEQNLNNSKFIGVVGTRKATDNGKDICRKLIHDLSSKLPNTTIVSGLAYGVDI
                     CAHKAAIDFDMATIGVLGHGLDRIYPAVHRATAVKMVQRGGLLTEYMSETNPDRQNFV
                     QRNRIIAGLCDALIVVESAQSGGALITAELANDYNRDVFAVPGRVGDEWSAGCNKLIR
                     ENKASLIESADDLLRFMSWESVDKPAGNTIQTSLFADLSEPEEAIIGILRQFAEGINV
                     NELAIQLNIPFSKLSSQLLEMEFKGLVKCLPGG"
     gene            complement(1079..1477)
                     /locus_tag="PKH68_02200"
     CDS             complement(1079..1477)
                     /locus_tag="PKH68_02200"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNX11777.1"
                     /translation="MIKNFVSTYWKSMLVVLIITVLSVYPFSSGLNIPHFKFQDKLIH
                     TFMYVALAFFLWYDTYSACNPKSGGRLLLSIILLFPTIYGGFIEIIQDLFFPPRTAEW
                     LDWAADIVGSVIGFMLALFLLRKKWKKTNY"
     gene            1640..2431
                     /locus_tag="PKH68_02205"
     CDS             1640..2431
                     /locus_tag="PKH68_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX11778.1"
                     /translation="MKKVLPLILLAVFFTACSSVMLTGRKQLNLVSDAEINAMSFQSY
                     KQFIDSVPLSTSKTNTAMVKRVGTNIANAVETYMKANGYEADIANFAWEFNLVKDTSV
                     NAFCMPGGKVVAFEGILPKTLTESGMAVVMGHEIAHAVARHSSERLSQQMMLQYGSAL
                     TGVLLSGKSAAVQQGIGALYGIGAQLGVLLPYSRKQEYEADKLGLIFMAMAGYNPTDA
                     LSFWQRMSADKSGSVSDFMSTHPSDEKRIARLQEELPAAMAYYKK"
     gene            complement(2511..3161)
                     /gene="upp"
                     /locus_tag="PKH68_02210"
     CDS             complement(2511..3161)
                     /gene="upp"
                     /locus_tag="PKH68_02210"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNX11779.1"
                     /translation="MKIINLSEKNSVLQHYLKEIRSVEIQKDSMRFRRNMERIGEIMA
                     YEISKTLNYKADKVKTPLGIKETTKIEEKLVVSTILRAGLPFHNGFLSYFDNAENAFV
                     SAYRKYKDALKFDIFIEYIASPKLDGKTLIITDPMLATGGSMELAIGALQTKGKPRKV
                     IIATIIASQPAIDYMQTHFGDDITLWTADIDPELNDHSYIVPGLGDAGDLAFGEKL"
     assembly_gap    3165..3264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3265..>4779)
                     /locus_tag="PKH68_02215"
     CDS             complement(<3265..>4779)
                     /locus_tag="PKH68_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX11780.1"
                     /translation="EPNKKALLWTNDKGDCREFTFAEIKKYTDQTASYFQTLGLKKGD
                     IVMAILKRRYEFWFTIIALHKLGITIIPATHLLTKKDIVYRNNAADITTIIAVGEEPI
                     ASAISEALAESPSIKHLISVGPIIPEGWEDFHRGIENAPEFTPIPKVNSNDDSMIISF
                     TSGTTGNPKMVTLDFVYPLAHIITAKYWHNLDESSLHLTIADTGWLKAVWGKLYGQWI
                     VGACVFVYDHDKFAPADMLQMIEKYKVTSLCAPPTIFRFLIREDLTKYDISSLKYCTI
                     AGEALNPEVYKQFYNLTGIKLREAYGQSETTVSVLTPPWIEPKPGSMGLPSPHYDVDL
                     LAPDGSPAEVGEQGQIVIRTDKGYPVGLFKGYHRNQQLTDECYSDNIYYTGDVAWRDE
                     DGYFWFVGRADDVIKSSGYRIGPFEVESALMTHPAVVECAITGVPDEIRGQVVKATII
                     LSREYKDKAGDELVKEIQQHVKTVTAPYKYPRIIEFVEELPKTISGKIRRKEIRD"
     assembly_gap    4780..4879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4880..5346)
                     /locus_tag="PKH68_02220"
     CDS             complement(<4880..5346)
                     /locus_tag="PKH68_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX11781.1"
                     /translation="MNHQIKQIAARLRGLRDALELSTEEFALKCEIPVQDYELWESGE
                     SDIPMSFIFHVAQTFGIDTTELLSGETAHTNAFFVTRKGKGISVERTKAYKYQALGAG
                     FSGNRAELFEVTVEPNDEPMTLNTHTGQEFNFVLQGTMQLHVAGNDLVLNPGD"
     gene            complement(5360..5977)
                     /locus_tag="PKH68_02225"
     CDS             complement(5360..5977)
                     /locus_tag="PKH68_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11782.1"
                     /translation="MMRTFLKIISYIFQPLLMPLYGMILLMQTNLFSFFAPTYKIIAL
                     AGTLLFTGLLPALPIYMMMKRGQIKDLFISKREERTMPYLFSMLSYLFWLFFLWRTLQ
                     FPPEFALVAAGSVLSVFLMVFINLKWKISAHLAGMGGFVGGVFAYSYLMAVNPIGLIV
                     ASVIISGLVAVSRVYLKAHTLSQTLAGFCLGFLCVFLPGVIYQLI"
     gene            complement(6002..7450)
                     /gene="rpoN"
                     /locus_tag="PKH68_02230"
     CDS             complement(6002..7450)
                     /gene="rpoN"
                     /locus_tag="PKH68_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNX11783.1"
                     /translation="MLKQQLQLKLQQRLSPAQIQVIKMLEIPTLELEERIRQEIEENP
                     ALEEGVEKSDDSDEQDDFNEDFDTNDTPDFEEYMADDDIPDYKLIANNSSRDDKHEDI
                     PFSAGLTFHEFLSDQVGLLQLTDRERSLMDYIIGNIDEEGYLRRSPESMVDDIVFQAG
                     VETTDEEMKRLVREVQQFDPAGVGAINLQECLILQLERREQTPDVINARKIITNFFEE
                     FSKKHYDRIIRAMAITEVELKEALNQIVKLNPKPGNAWSGNVLEKTFSAIVPDFIIEN
                     DEGTLTVQLNNSNVPELRVSSTYNEMFRDFTSNKENQSREMKDAVMFAKQKIDAARWF
                     IDAIKQRQQTLIATMTAIVNYQREFFIEGDETYLKPMVLKDIADITGYDISTISRVSS
                     SKYAQTEFGIFPVKFFFSETMLNDSGEEISTREIKKIMQECIDEEDKRAPINDDKLAE
                     MLKEKGYNIARRTVAKYREQLNIPVARLRIEI"
     gene            7700..>8116
                     /locus_tag="PKH68_02235"
     CDS             7700..>8116
                     /locus_tag="PKH68_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HNX11784.1"
                     /translation="MYSKDELKQLKKEFWEGFGIYCSNHPVLGKRKSKFMLYNTKMKG
                     VELKFDATREGAFVILELNSLNETERLAKFELLEQYKMLMEKNFPAGLIWNFAYELDS
                     GREVCRIYSQKTGIDIHRRIHWMEFYRFMAEEMLKLE"
     assembly_gap    8117..8216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8395..10353
                     /gene="dnaG"
                     /locus_tag="PKH68_02240"
     CDS             8395..10353
                     /gene="dnaG"
                     /locus_tag="PKH68_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNX11785.1"
                     /translation="MIDQATIDRINDAAQIVDVVSDYVTLRRRGVNMIGLCPFHNEKT
                     PSFTVSPAKGIFKCFGCSEGGNSVHFIMKQEQLSYYEALKFLAKKYHIEVRERELSDE
                     EQAARNDRESMLLVNEFAQKYFTNTLHHHIDGKAIGLGYLRERGFRDDIILKFQLGYC
                     LDERDAFTKSAVNAGYNKDFLVKTGLTIEGENNYVADRFRGRVMFPVHSLSGKVLAFG
                     GRVLKKDEKTAKYVNSPESEIYHKSNELYGIYFAKQSIVKHDRCFLVEGYTDVISMHQ
                     SGIENVVASSGTSLTPGQIRLIHRFTENVTVLYDGDPAGIKASIRGIDLLLEEGLNIR
                     VLLLPDGDDPDSFSRKHNASDFIEFVNQHSVDFMQFKTNLLLHDAGNDPIKRASLVQD
                     IVRSIAMIPNQIIRSEYVKECSTLLNVDEKLLYHEINKIKNTELEAKILKKTVDEKRE
                     TIPDLSAPANTEPKSENKFNNEERNLVRALIKFGSDVLFVSKPDENSTETPVTVSDYI
                     LGELQIDQLTFRNELHEKIRQEYIQHSTRENFNAERFFINHPEVEVSKLAADLISEKY
                     ILSKIHSKFHKLETETDRLAQLAPRFVFEYKLAVIMEKRKEKLMEMKTAAETRNEKLM
                     LQLMNEIEAIDNFKNMLSKELDRVLNLI"
     gene            10419..11663
                     /locus_tag="PKH68_02245"
     CDS             10419..11663
                     /locus_tag="PKH68_02245"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNX11786.1"
                     /translation="MENLWQQDTLKAARLLNLITEEKINAVLLTLADEAVAATGDILA
                     ANALDLAALETSNPLYDRLLLTPERIKSIANDIRNVAALPSPLGKILMETERPNGMKI
                     KKISVPFGVVGIIYEARPNVSFDVFSLCFKSGNTCVLKGGSDAFNSNRAIVELIKNVL
                     RKHELDENTVTLLPAGREAAAELMNAAGTVDVIIPRGGKGLIDFVRKNSLVPVIETGA
                     GVCHTYFDEFGDLQKGKDIVFNAKTRRVSVCNALDCLIIDQKRLIDLSLICQKLAEKN
                     VTIYADEPAYRALNSHYPDSLLKMTDEYSYGTEFLDYKMAVKTVAGVREAVEHIATYS
                     SQHSECIITENAATAEYFTKVVDAACVYTNVSTAFTDGAQFGLGAEIGISTQKLHARG
                     PMGLAELCSYKWVIKGEGQTRS"
     gene            12059..12304
                     /locus_tag="PKH68_02250"
     CDS             12059..12304
                     /locus_tag="PKH68_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNX11787.1"
                     /translation="MNIYFGNLSYKVRENDLQGVVEEYGEVSSCKIIKDRDTGKSKGF
                     AFVEMADDAAATKAIEELNGAELDGRTLVVKEARPKA"
     gene            12414..13295
                     /locus_tag="PKH68_02255"
     CDS             12414..13295
                     /locus_tag="PKH68_02255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNX11788.1"
                     /translation="MLKETFRNSGTLGKLLQLFFITLFLGIAAMGIVSIIFDSTALIN
                     DQKVMQLILSLFTFLIPPVVVGYLWYERPAAAYGLTQKPDGKQIILAVLLMIALSPGI
                     NLLAYLNEQIGLPDFMKGIEQYFKDMEALASSVTEQMLKVNNIGDLMVNLLVMAVVPA
                     FCEELYFRGTLQNIFSEKRNKHAAIWIVAIIFSLIHFQMYGFIPRMILGAVLGYLLVW
                     SGSLWVPVIAHFTNNALAVLVSGFGQGNEQVKAMEEIGKSETYWYGIISILISALIIR
                     EIYKMRKKETQEIKPVS"
     gene            complement(13307..13696)
                     /locus_tag="PKH68_02260"
     CDS             complement(13307..13696)
                     /locus_tag="PKH68_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNX11789.1"
                     /translation="MVALLSLLILAGLALFLVTQVARSRRKEEVEIVINEDPECCGAH
                     EVCERDNLQIKDTKIEYFEDEELDALAGLAPEKFSDEQTEAIAYVLYSLRESDVASWL
                     RSLQLRNIQLPASLREQALMIVGERRA"
     gene            13872..17336
                     /locus_tag="PKH68_02265"
     CDS             13872..17336
                     /locus_tag="PKH68_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX11790.1"
                     /translation="MKIRTLHIGYLLLAVLLMSACSTKKNTAATRNYHALTTRFNIGF
                     NGSESYREGLENIEKANKDDYTRVIPMYPIGNKASATAAVSNMDRTIEKCRKAIKLHS
                     IRKKPQKNYKKANDPKYLSFYNQNEFNPALRQAWMLLGQAEFHKGDFLGSVGTFSYIG
                     RYYASVPQMVVQSQIWTARAYTEMDWLYEAEDVLQKINQDQITGKNTGLYAAVSANLM
                     IKKGQYKEAIPFLKLAIENEKNKKLRTRFNFVMAQLQERTGDKVAASDYYTEVIKSNP
                     GYELNFNARINRAQLLARNVKSIEKELKKMARNRNNRDYLDQIYAALGNMYLQEKDTV
                     KAISNYNLALEKSKRNGIDKAVAALKLGELYYNRRDYVKAQPCYDEASKIFTNDYADY
                     ASISKRAEMLGELVKEHEIIVLQDSLQALAMLSDDKRLEAINRVIEKVKKEEKEEAEN
                     KKNPQQGSRGMNDPEDDFAMNMGAPIGGGNNRNWYFYNPVTINSGKADFKKKWGNRKL
                     EDNWRRSNKSAALFADNTGSTPASSDNPLTTGTDSVQTPKIKATSDKTSVDYYLKQLP
                     FTAEQRKKSDAEVADAMFAKGFIFKDKLEDFPMAYSTFEEFQRRFGNDERVVETLFQC
                     YLMASKEKNESQAEAYRNRILTAFPNSKMAQLLKDPDYIRRQGSMFAEQDSLYSQTYA
                     AYTRSDFSTVFKNTDYFRKKYPLAALLPKFEFLNALSIGKTAASTEFEKSLSKLVESY
                     PQSDVSAMSKDILALMKQGNLAQKGTSHGTLLSKRAAVDSTNIARDAAMSFSPDKEGK
                     HRLLLLTDATSDGINKLLYNLAIFNFSRFMIKDFDFVVGPADATHQGISVTNLESYDE
                     VLWYQKTLKTDQSLTALMDSLKVTQVVISEDNFGKMKSIFSLDEYLAFEKNNLMKEKP
                     ANLAVSTTSTPSKKEVAKKPATETLQASPVKEVAVTAASTTPSAQKTTTPSEPKTVAA
                     IDQKAAAQPAKNIETTKTVTTDKTATAVNKQPAASEPVKTAEKAEPEKPAVMEEKVEW
                     YKNLFAIRLNAPHFVAFYLPPGKFDFPKIQKALDAYNAANYSTMNLAVNLEDPGKDKF
                     IIVGTFTDANVAKSYFMRMLKDPGITEAFRGVNRRNLVGTKENLNIMVKKDALNTYFE
                     FMREYYLK"
     gene            17466..19013
                     /locus_tag="PKH68_02270"
     CDS             17466..19013
                     /locus_tag="PKH68_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNX11791.1"
                     /translation="MKKDRILWADDEMDLLKPYLLFLEEKGYEVVAVTNGKDAIEICQ
                     NESFDIIFLDENMPGLSGLETLAQIKVLDPNVPMVMITKSEEEDIMNMAIGNKIADYL
                     TKPVNPSQILLTLKKNIHKKEIVSEHTSTSYQQQFRNIGMQINESLTYMDWVDVYKKL
                     VYWELELAETENGMDEMLKMQKNEANSTFNKFVKKNYLDWMSNPALRPLMSTDLFKTK
                     VFPLLDKGEKVFFVLVDNFRYDQWIIIRELLSEFYTFTEEETYCSILPTATQYARNAI
                     FSGLMPLQIQQMYPHLWIEEEDEEGKNINEEALLQTQLDRFRKKYNFSYHKVFESGYC
                     EKVINQLPSLENNQLNVVVLNFIDMLSHARTESKMMRELANDEKAYRSLTLSWFRHSA
                     TYDLFKAIAQRGYKVIVTTDHGTIQVNNAIKVVGDKNVNTNLRYKVGKNLSYNKKEVF
                     EILQPQKAGLPSPNVSSAYIFAGNDDFFAYPNNFNHYVTYYKNTFQHGGISLEEMIIP
                     VVTMEPK"
     gene            19285..20238
                     /locus_tag="PKH68_02275"
     CDS             19285..20238
                     /locus_tag="PKH68_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HNX11792.1"
                     /translation="MKTYTNVKDLGNLQEAVKEALEIKNNRYAYKQLGENKTLLMVFF
                     NSSLRTRLSTQKAGMNLGMNTMVLDINQGAWKLETERGVIMDGEKPEHILEAIPVMGT
                     YCDIIGVRAFAQFENKEFDYQETIINQFIQYSGKPVFSMEAATGHPLQAFADLITIEE
                     YKKTARPKVVLTWAPHPKALPQAVPNSFADFMNEADVEFVITHPIGYELSEKFVRGAR
                     VEYDQMKAFEGADFIYAKNWAAYQDPNYGKILSTDRNWTVSDRQMAVTNNAYFMHCLP
                     VRRNMIVTDDVIESPQSIVIPQAANREISAQVVIKRLLENI"
     gene            20249..>20546
                     /locus_tag="PKH68_02280"
     CDS             20249..>20546
                     /locus_tag="PKH68_02280"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine phosphoribosyltransferase family protein"
                     /protein_id="HNX11793.1"
                     /translation="MNLEQVIASIRNVPDFPKPGIQFKDITTAMKDAEVLHYISDTLY
                     EYYKDKGITKVVGVESRGFMLGSILAYKLNAGFVLLRKKGKLPAETYSINYALEY"
BASE COUNT         6003 a         4487 c         4421 g         5335 t
ORIGIN      
        1 cctccgggca ggcacttcac cagtcccttg aattccattt cgagcaattg cgaggagagt
       61 ttgctgaagg gtatattgag ttgaatggcc agttcgttga cattgatacc ttcggcaaac
      121 tggcggagta tgccgatgat ggcttcttcc ggttccgaca aatcggcaaa caaggaagtc
      181 tggatggtat tcccggctgg tttatccacg ctctcccagc tcataaaacg cagcaggtca
      241 tcggccgact ctatcagcga tgctttattt tcccgaatca gtttattaca ccccgcggac
      301 cactcgtctc ccaccctgcc gggcacggca aaaacgtcgc ggttgtaatc attggcgagt
      361 tcggctgtga tgagtgcgcc tcccgattgt gctgattcta ccacaatcag tgcatcgcac
      421 agcccggcaa taatgcggtt tcgctgtaca aaattctgcc ggtcgggatt ggtttcactc
      481 atgtattcgg taagcaatcc gccgcgttga accattttaa cagcggtggc acgatgcact
      541 gccggatata tccgatccag accatgtccc agaacgccaa tagtggccat atcgaagtcg
      601 atcgctgctt tgtgggcaca tatatccacc ccgtaagcca aaccgcttac aatcgttgta
      661 tttggcaatt tcgaagacag gtcatgaatt aactttctgc atatatcttt tccgttgtcg
      721 gtggcttttc gcgttcctac tacgcctata aattttgaat tgttcagatt ttgttctcct
      781 ttgtaataca acattgcagg agcatctgcg cattccttca gccggtatgg atacgacttt
      841 tcggtataaa acatcgtcag aatattgttt ttaacgacaa aattcacttc cttttcggct
      901 cgtgcctgca catcctgcga aacaatttcg cgagacaaca cctcaccaat tccgggaatt
      961 ttggccagat tttgccgctt ttccctgaaa accccttcca cagaacctac gtaagctatc
     1021 agattcttgg ccaggttggg tccgatgcct ttgatgaggg ttatgcctat ctgatatttt
     1081 aatagttcgt ctttttccat ttcttacgga gtaaaaataa tgcaagcata aaaccaatta
     1141 ccgatccgac aatatcagct gcccagtcga gccattcagc ggtacgtggc gggaaaaata
     1201 aatcctgaat aatctcaata aaaccaccgt aaatggtcgg aaaaagcagt atgatggata
     1261 gcaataatcg tcccccggat ttcggattgc aggcactgta cgtgtcgtac cagagaaaaa
     1321 aagcgagcgc aacgtacatg aaggtatgaa tgagcttgtc ctgaaattta aagtgaggta
     1381 tattcagacc tgatgaaaag ggataaacgg aaagaacagt aatgatcaga accaccaaca
     1441 tggatttcca ataggtagaa acgaaatttt ttatcatcaa cgggtatttt tttcaccggg
     1501 aatattactg ataaattccg ggcataattt ttgtaaataa aacttcaggt caaatataat
     1561 aattcaaaag cttttattag atttgcaaag ataatttttt aggataaata tacaccgttc
     1621 aattattaaa attttaatca tgaaaaaagt tcttccgttg atactgcttg cagttttttt
     1681 taccgcctgt tcaagtgtaa tgcttaccgg acgtaaacaa ttgaatctgg tctccgatgc
     1741 cgaaattaat gccatgagtt tccaaagcta taaacaattt attgattctg tgccactttc
     1801 caccagcaaa acaaatactg ccatggtaaa aagagttggc accaacattg ccaatgctgt
     1861 agaaacttat atgaaagcca atggctacga agccgacatc gccaattttg cctgggaatt
     1921 taatctcgtt aaagacacct ctgtcaatgc attctgtatg cccggcggaa aagtagttgc
     1981 attcgaagga attttaccta aaaccctcac cgaatcaggt atggctgtag tgatgggaca
     2041 cgaaattgct catgcggtag cccgtcacag cagcgaacgc ctcagccagc aaatgatgct
     2101 gcaatatggc agcgcgctga caggcgtact cctttcgggt aaatcggctg cagtacagca
     2161 aggtatcggc gcgctgtatg gaattggtgc ccaactgggt gtactgctcc cctactctcg
     2221 caaacaggaa tatgaagccg ataagctggg gttgatattt atggcaatgg ccggttataa
     2281 tccaaccgat gcactttcct tctggcaacg tatgtcagcc gacaagtcgg gctctgtatc
     2341 cgactttatg agcactcacc cttcggacga aaaacgaatt gcccgcctgc aggaagaact
     2401 tcctgctgca atggcttatt acaaaaaata acaacagaat ttattctaat aaaaaaacag
     2461 acaggtgaac gttcacttgt ctgttttttt tgtcaattaa atgtcctgca tcagagtttt
     2521 tctccgaaag ccagatcgcc ggcatcgccc agaccgggaa caatgtacga atgatcattc
     2581 agttcgggat caatatcggc agtccagaga gtaatatcgt cgccaaaatg agtttgcata
     2641 tagtcaatgg ccggctgact ggcaataatg gtagcaataa tcactttacg gggcttaccc
     2701 ttggtctgta aggctccgat tgccagttcc atagagccgc cggtggcaag cattggatcg
     2761 gtgatgataa gagttttacc gtcaagtttt ggcgatgcga tatattctat aaaaatatca
     2821 aacttgaggg cgtccttata tttgcggtaa gccgatacaa aagcattttc agcattgtca
     2881 aaataactga gaaaaccgtt gtgaaaaggc agtccggctc gaagaatcgt tgaaaccact
     2941 aacttctcct caattttggt ggtttcttta atacccagcg gagtttttac cttatcggcc
     3001 ttataattca gcgttttact tatttcatat gccattattt cgccgatacg ttccatatta
     3061 cggcgaaagc gcatggagtc tttctgtatt tccacagatc tgatttcttt gagataatgc
     3121 tgaagtactg agtttttttc ggatagattg atgattttca tattnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnatcgcg gatttctttt ctgcgaatct ttccactgat
     3301 agtctttggg agctcttcca caaattcgat tattcgcgga tatttgtagg gtgcagtcac
     3361 cgttttaaca tgttgctgga tttcctttac cagttcgtca ccggctttat ccttgtattc
     3421 acgcgataaa atgatggttg ctttcaccac ctgtccgcgg atttcgtcgg gcactccggt
     3481 aatggcgcat tccactaccg caggatgtgt catcaaagca ctttccacct caaagggacc
     3541 gatgcggtaa cccgagcttt taatcacgtc gtcggcacgg cccacaaacc agaaataccc
     3601 atcttcgtcg cgccaggcta catcgccggt gtagtatata ttgtcgctgt aacattcgtc
     3661 agtcagctgc tgattgcggt gataaccttt gaataatccc acgggatatc ccttgtcggt
     3721 gcggattact atttgtccct gttcacccac ttcggctggg ctgccgtcgg gagcaagcag
     3781 atccacatcg tagtgtggac ttggcaggcc catcgaaccg ggttttggct ctatccaggg
     3841 cggagtgagc accgatacgg ttgtttcgct ctgtccgtaa gcttcccgca gtttgatgcc
     3901 ggtaagatta tagaactgtt tgtacacctc cggattgagt gcttcaccgg caatggtgca
     3961 gtatttcaac gaagaaatgt cgtattttgt caggtcttcg cgtatcagaa agcggaatat
     4021 ggtcggaggt gcacagagcg atgtaacttt gtatttttca atcatttgaa gcatgtctgc
     4081 aggagcaaat ttatcatgat catatacaaa cacacaagcc ccgactatcc actgtccgta
     4141 tagttttccc catacggctt tgagccagcc ggtatcggca atggtgagat gaaggctgct
     4201 ctcgtcgagg ttgtgccagt atttagcggt aatgatatgt gccagcggat aaacaaagtc
     4261 gagcgttacc attttcggat ttccggttgt accggaggta aaactgataa tcatcgaatc
     4321 gtcgttgctg ttcactttcg ggattggcgt aaattcggga gcattctcta ttccccggtg
     4381 aaaatcttcc cacccttcgg gaatgatcgg ccctacggat atcaggtgtt tgattgatgg
     4441 agattcggca agcgcttcgc tgatagccga agcaatgggt tcctcaccca cagcaatgat
     4501 ggtggtaata tctgctgcat tattccgata aacaatatct tttttggtca gtaagtgtgt
     4561 tgccggaata atggtaatgc ccagtttgtg aagtgctata atggtaaacc agaattcgta
     4621 ccggcgtttg agaatggcca tgacaatatc tccttttttc aatcccaggg tctgaaaata
     4681 ggatgccgtt tggtcggtat atttttttat ttcggcaaat gtaaattcac gacaatcgcc
     4741 cttgtcattg gtccaaagca gtgctttttt gtttggttcn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnc tgtcgcccgg attaagcacc agatcgttgc ccgccacatg
     4921 gagctgcatg gtaccctgca acacaaagtt aaattcctga ccggtatgtg tattgagggt
     4981 cattggttca tcgtttggct ctacggtgac ttcaaaaagt tcagcccggt tgcccgaaaa
     5041 tccggctccc aatgcctggt atttgtatgc tttagtgcgt tccacactta tcccttttcc
     5101 ctttcgggtc acaaaaaaag cattggtatg ggctgtttcg cccgacaaga gttcggtggt
     5161 atcgattcca aaagtctggg caacatggaa aataaaactc atggggatgt ccgattctcc
     5221 actttcccat agttcgtaat cctgtacggg tatttcacac tttagggcga actcttcggt
     5281 gctgagttca agcgcatcgc gcaatccccg caatcgtgca gctatttgtt tgatctgatg
     5341 attcataatc tcttgatctt tatataagtt gataaattac accgggtaag aaaacacata
     5401 aaaatcccag acaaaaaccg gccagtgtct gcgaaagggt gtgcgctttg aggtaaactc
     5461 tggaaacggc cacaagtcct gaaatgataa cagaggctac gatcagcccg atgggattga
     5521 ccgccataag gtatgaataa gcaaaaactc cgcctacgaa accacccatt ccagccaggt
     5581 gagcactgat tttccatttc agattgatga ataccatgag aaaaaccgac aataccgacc
     5641 cggctgccac cagcgcaaat tcgggaggga actgcagcgt tctccacaga aaaaacagcc
     5701 aaaacaaata tgaaagcatc gaaaaaagat agggcatggt tcgttcttcg cgtttggaaa
     5761 taaacaaatc cttgatttga ccacgtttca tcatcatata aatgggtaaa gcaggcagca
     5821 atccggtgaa gagaagtgta ccggccaaag ctatgatttt atatgtggga gcaaaaaaac
     5881 tgaacagatt ggtctgcatc aaaagaatca ttccatacag aggcatcaac aatggttgaa
     5941 aaatatagga aattatcttc aggaatgttc tcatcatttt ttatttttta cagtatctta
     6001 tttatatctc aattctaagt ctggccaccg gaatattgag ttgttcacgg tatttggcca
     6061 ccgtacggcg ggcaatgtta tatccttttt ccttgagcat ttcggccagt ttatcgtcgt
     6121 tgatgggtgc ccgcttatcc tcttcgtcaa tgcattcctg cattattttt tttatttcgc
     6181 gtgtcgaaat ctcttcaccg gagtcattca acatggtttc ggagaaaaag aattttacag
     6241 ggaaaattcc aaattcagtt tgggcatact tgctgctgct cacccgcgaa atggtggaaa
     6301 tatcgtagcc tgtaatatcg gcaatatctt tgagcaccat gggtttgagg taggtttcgt
     6361 ctccttcgat gaaaaattca cgctgataat tgacaattgc cgtcatagtg gcaatgagcg
     6421 tttgctggcg ttgtttgata gcatcgataa accagcgtgc tgcatctatt ttttgcttgg
     6481 caaacatcac ggcatctttc atctcgcgcg actgattttc tttattggaa gtaaaatcgc
     6541 ggaacatttc attgtaggta ctgcttacgc ggagttcggg aacattgctg ttgttgagct
     6601 gaacggtgag tgtgccttca tcattttcaa taataaaatc gggtacaatt gccgagaagg
     6661 ttttttcgag cacattgcca ctccaggcat tgccgggctt gggattgagt tttactatct
     6721 gattgagagc ctctttcagt tcaacttcgg tgatagccat cgcacggatg attctatcgt
     6781 aatgtttttt ggagaattct tcaaaaaagt ttgtaatgat ttttcgtgca ttgatcacat
     6841 cgggagtctg ctctcttctt tcgagttgta gtatcagaca ctcctggaga ttgatagcac
     6901 ctacaccagc aggatcaaac tgctgcactt cgcgtaccag ccgtttcatc tcctcatcag
     6961 tagtctccac accggcctga aatacgatat cgtccaccat cgattcgggg gagcgtcgca
     7021 gatacccttc ttcgtcaatg ttgccgatga tgtaatccat caggcttcgc tcacgatctg
     7081 tcaactgtaa caatccgacc tgatccgaca agaattcatg aaaagtgaga ccggctgaaa
     7141 atggaatatc ctcgtgcttg tcgtcgcgcg agctgttatt ggcaattaat ttgtagtcgg
     7201 gaatatcatc gtcggccata tactcttcaa aatcaggagt atcgtttgta tcgaaatctt
     7261 cattaaaatc atcctgttcg tcgctgtcat ccgatttttc gacaccctct tcgagggccg
     7321 gattttcttc tatttcctgg cggatacgtt cttccagctc caaagtagga atttcgagca
     7381 tcttgatcac ctgaatttga gcaggcgata gtctttgctg aagttttagc tgaagttgct
     7441 gttttaacat aaatatattt tactgactgt gagttatcct atccctgttt catccgagta
     7501 caaaggtacg ttttttcgtt taaaaaataa cgaacagcac ccattttaaa cctttacaca
     7561 actttgcagc ctcatatagg gagtcttccc atcaccgcgt tgaaatctgt tctgtttgtt
     7621 taacagtatt ctttttttga aaagttgaat taaatgagta gttttgcaca ggtattttac
     7681 ttcaatttcg gcatcggtca tgtacagcaa ggacgaatta aaacaactga aaaaggaatt
     7741 ttgggagggt tttggtattt attgcagcaa tcatccggta cttggcaaaa ggaaaagtaa
     7801 atttatgctg tacaacacca aaatgaaagg tgtggaactc aaatttgatg ctacccgcga
     7861 aggtgctttc gttattctgg aattaaattc actgaatgaa acggagcggt tagccaaatt
     7921 tgaactgttg gaacagtata aaatgctgat ggaaaaaaat tttcctgccg gactgatttg
     7981 gaactttgca tatgaacttg attctggtcg ggaagtctgc cggatttaca gtcagaaaac
     8041 gggcatagat attcaccgcc gcattcactg gatggaattt taccggttta tggctgaaga
     8101 gatgcttaaa ttggaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg
     8221 aagagatgct taaattggaa aaagcattca aaaattgccc cctaaccccc tgataaatag
     8281 agacaggctc tgatggtggt atctgcagca tttttcaaga gtatatgaaa taaaaaagga
     8341 ttaataataa ttaatgattg aaagcctccc cttcggggag gttggagggg ctcaatgatt
     8401 gaccaggcta ccatagaccg gataaacgat gctgcacaaa tagtagatgt ggtgtccgat
     8461 tacgttacac tgcgccggcg cggggtgaac atgatcggtc tgtgtccgtt tcacaatgaa
     8521 aaaacgccgt cgtttaccgt ttcgccagcc aagggaattt tcaaatgttt cgggtgcagc
     8581 gaaggaggta attcggtgca tttcatcatg aagcaggagc agctttcgta ctacgaagcc
     8641 ctgaaatttc tggccaaaaa ataccacatt gaagtacgcg aacgcgaact cagcgacgag
     8701 gaacaggcag cccgcaacga ccgcgaaagc atgttgctgg tcaatgaatt tgctcaaaaa
     8761 tactttacca ataccctcca ccatcatatc gacggaaaag ccatcggtct gggttatttg
     8821 cgcgaacggg gattccgtga tgatatcatc ctgaaatttc agctcggtta ctgcctcgac
     8881 gaacgggacg cttttaccaa atcggctgtc aatgccggtt acaacaagga ttttctggtt
     8941 aaaaccggtc tgaccattga gggggaaaac aactacgttg ccgaccgatt ccggggccgg
     9001 gtgatgtttc ccgttcattc actctcgggc aaagtgctcg cctttggcgg gcgggtgctc
     9061 aaaaaagacg aaaaaacggc caaatatgtc aactcgcccg aaagtgagat ttaccataaa
     9121 agtaatgagc tatacggaat ttattttgcc aaacaatcta tagtaaaaca cgaccggtgt
     9181 tttctggtgg agggttatac cgacgttatc tccatgcatc agagtggcat cgagaatgtg
     9241 gtggcatcgt cgggcacatc gcttacgccg ggtcagatac gacttattca ccgctttacc
     9301 gaaaatgtaa cggtgctcta cgatggtgac ccggccggaa ttaaagcatc catccggggt
     9361 attgacctgc tgctggaaga agggctcaac atccgcgtgc ttctgctgcc cgacggcgat
     9421 gatcccgatt cgttttcgcg caagcacaat gcatccgatt ttatcgaatt cgtaaatcag
     9481 cattcggtgg atttcatgca gttcaaaaca aacctactgc tccacgatgc cggcaatgac
     9541 cccatcaaac gggcttcgct ggttcaggac attgtacgaa gcatagccat gatccccaat
     9601 cagataatcc ggtcggaata tgtgaaggaa tgcagcaccc tgctgaatgt ggatgaaaaa
     9661 ttgctttatc acgaaatcaa taagatcaaa aataccgaac tcgaggctaa aattctgaaa
     9721 aaaacggtgg acgaaaaaag ggaaaccatc cccgacctgt ccgcacccgc aaacacagaa
     9781 cccaagagcg aaaataaatt caataacgaa gagcgaaatc ttgtacgggc gctgatcaaa
     9841 ttcgggtccg acgttctgtt tgtatcaaaa ccggacgaaa acagcaccga aacacctgtt
     9901 accgtgagcg attatattct cggcgaattg cagattgacc aactgacatt caggaacgaa
     9961 cttcatgaaa aaatcagaca ggaatacata caacactcga cgagagaaaa tttcaacgcc
    10021 gaacgatttt tcatcaatca ccccgaagtt gaagtcagca aactggccgc cgacctgatt
    10081 tcggaaaaat atatcctgag taaaatacat tccaagtttc acaaacttga aaccgagacc
    10141 gaccggctcg cccagctggc tccgcgattt gtatttgagt acaaactggc tgttatcatg
    10201 gaaaaacgca aggaaaaact gatggaaatg aaaacagctg ccgaaacccg caacgagaaa
    10261 ctgatgcttc aactaatgaa tgaaatagaa gcaattgaca acttcaaaaa tatgctttcg
    10321 aaagaactcg accgggtttt gaatttaatt tagcagaaat attttttctt ttttttggaa
    10381 gggaaaaaat gtatgacaat caccaaatta tgattattat ggaaaattta tggcaacagg
    10441 acaccctgaa agctgcccga ttgctcaacc tcattaccga agaaaaaatt aatgccgtat
    10501 tgctgacact ggccgacgaa gcggtggctg ccaccggtga tattctggct gccaatgcac
    10561 ttgatctcgc tgcactggaa acttcaaatc ctctgtatga ccggctgctt ctcactcccg
    10621 aaagaataaa aagtatagcc aacgatatcc gtaatgtggc tgctctgccc tcgcccctgg
    10681 gcaaaattct gatggaaacc gaaagaccaa acgggatgaa aattaagaaa atatcggttc
    10741 cgttcggcgt ggtgggcatc atatacgaag cccgccccaa tgtgagtttc gatgtgtttt
    10801 cactttgctt caaatcgggc aatacctgcg tgctgaaagg tggcagcgat gccttcaact
    10861 caaaccgggc tatcgttgaa cttatcaaaa atgtactgcg aaagcatgaa cttgacgaaa
    10921 atactgtaac actgcttcct gccggacggg aagcggctgc cgaactgatg aatgccgctg
    10981 gtacggtaga tgtgatcata ccgcgtggtg gaaaaggatt gattgatttt gtacgaaaaa
    11041 actcactggt gccggtcatt gaaacaggtg ccggtgtttg tcatacctac tttgatgaat
    11101 tcggtgattt gcaaaaagga aaagacatcg ttttcaatgc aaaaacacgc cgtgtaagcg
    11161 tgtgtaatgc cctggactgc ctgataattg accaaaaacg attgattgat ttatcgctga
    11221 tttgccaaaa actggctgaa aaaaacgtaa cgatatatgc cgatgaacct gcttaccggg
    11281 ctctgaacag ccattatccc gatagtctgc tgaaaatgac tgatgaatac agttacggta
    11341 ctgaatttct ggattataaa atggctgtga aaacagttgc cggagtccgc gaagcggtgg
    11401 agcatatcgc cacttacagt tcgcaacaca gcgagtgcat cattaccgaa aacgctgcaa
    11461 cagccgaata ttttacaaaa gtggtggatg cagcctgtgt ttacaccaat gtttccacag
    11521 cctttaccga tggagcacag tttggactgg gagccgagat aggcataagc acgcagaaac
    11581 tgcatgcccg cggaccgatg ggactggcag agctatgttc gtacaaatgg gtcatcaaag
    11641 gcgaaggaca aacacgcagt tgatgcaatt ttattctatg cggtgaagtt ttcgagctct
    11701 tattcaacag cttctacgaa acggttttaa gtatgtgccc gggtttgtgt gtttttttca
    11761 aaatccaaga taacccgttt ttcatttcta ttctgcagta aatatgctgc aatatggaaa
    11821 taaacttcca ctttttcacc cataccggga acataattta caatatatca ataattttat
    11881 gtttttttgt tggaatttca aaaaaaatgt ttttctttgc ctttcgaaag aaaagtgtat
    11941 ttttcgtacg gtttaaagcc aacagttttc acctgatctt atgaatgact tttgtttttt
    12001 taccttccgt ttttatgcta tttctgtaac cttatttttt attcaatctt ttctcattat
    12061 gaacatttat tttggaaacc tgagctacaa agttcgggaa aatgaccttc agggtgttgt
    12121 agaagaatac ggtgaagtat cttcctgcaa aattatcaaa gaccgcgaca ccggtaaatc
    12181 caaaggattt gctttcgtag aaatggctga tgatgcagcc gccaccaaag ctatagaaga
    12241 acttaacggc gctgaactcg acggacgtac acttgtagta aaagaagcac gccccaaggc
    12301 ttgatttttt cacacaaaag gattaaaaca acttccattc cttctgaggg gtggaagttt
    12361 tttttcatac catttatttt taatactttt gtgttaaatt aaagaacagg aaaatgctta
    12421 aggaaacttt caggaattcg ggaacattgg gaaaactgct gcaattattt tttataaccc
    12481 tttttctggg cattgccgca atgggcatcg tgtcgattat tttcgactca acggccctta
    12541 tcaacgacca gaaggtgatg caactgatac tttctctgtt tactttttta attccgcctg
    12601 ttgttgtggg ctatttgtgg tacgaacgcc ctgctgcagc ctatgggctg actcagaaac
    12661 cggatggaaa acagattatt ttagcggttc tgctgatgat agccctctcg ccgggcatca
    12721 acctgctcgc ctatctcaac gagcaaattg gtctgcctga ttttatgaag ggtatcgaac
    12781 agtatttcaa agacatggaa gccctggcct cttcagtaac cgagcaaatg ctgaaagtga
    12841 acaacatcgg tgatttaatg gtcaatctgc ttgtaatggc cgtagtcccg gcattttgcg
    12901 aagaacttta tttcaggggt acgttgcaaa atatattttc tgaaaaaaga aataaacatg
    12961 ctgcaatctg gattgtggct attattttca gtctgataca ctttcaaatg tacggcttta
    13021 ttccgcgcat gattttgggt gctgtgcttg gatatctgct tgtgtggagc ggaagtttgt
    13081 gggtgccggt cattgctcac tttaccaaca atgcactcgc cgtactggta tccggtttcg
    13141 gacagggtaa tgaacaagta aaggcgatgg aggaaatcgg aaaatcggaa acttactggt
    13201 atggaataat cagtatcctg atttcggcat taattattcg cgaaatctat aaaatgcgga
    13261 aaaaggaaac tcaggaaata aagccggtat cataacaccg caaaatctac gctctcctct
    13321 cccccactat catcagtgcc tgctcgcgta gagatgcagg taactgaata ttccgcaact
    13381 gtaaactacg caaccaggaa gccacatcgg attcacggag cgaatacagc acgtatgcaa
    13441 tcgcttcggt ctgttcgtcg ctaaactttt cgggagccaa tccggccagg gcatccagct
    13501 cctcatcctc gaaatactca atttttgtat cttttatctg cagattgtcc cgttcgcaca
    13561 cctcatgcgc tccacaacac tcggggtctt cgttaattac aatttcaacc tcctctttcc
    13621 ggcgtgaacg tgccacctgt gtaactaaaa acagagccag tccggccaaa atcaacagcg
    13681 ataataaagc aaccatacga aggaaataat ttgggatttc agtgattaaa caactattat
    13741 catgcaaagt tagatattgt tggtcgaaaa attgtatttt tgtaggttaa aactttatta
    13801 ttcacacaat aaaacccccg tttgatggtt ttttgttatg ttgaattagt cccgaaaaac
    13861 aattatttat cttgaaaata agaaccttac atatcggtta tctcctgttg gctgtgttgc
    13921 tgatgagtgc atgttccacc aaaaaaaata cagcagcaac acgcaattat cacgccctta
    13981 ccacccgttt caacatcgga tttaacggct cggaaagcta ccgcgaaggg cttgaaaata
    14041 ttgaaaaagc caacaaagac gattacaccc gggtgatacc catgtacccc attggcaata
    14101 aagccagtgc aacagccgcc gtatcgaata tggaccgcac cattgagaaa tgccgtaaag
    14161 ccattaaact acattcaatc cgcaaaaaac cacaaaaaaa ttacaaaaaa gccaacgacc
    14221 ctaaatacct gtcattctac aaccagaatg agttcaatcc ggcattgcga caagcctgga
    14281 tgcttctggg tcaggcagaa tttcacaaag gcgacttttt gggttctgta ggtacatttt
    14341 catatattgg caggtattac gcatcggttc cgcaaatggt ggtgcaatcg caaatctgga
    14401 ctgcccgggc ttataccgaa atggactggc tatacgaagc cgaggacgta cttcaaaaaa
    14461 tcaatcagga tcagattacc ggcaaaaaca ccggattata cgcagcagtc agcgccaatc
    14521 tgatgataaa aaaaggacag tacaaggaag ccataccatt cctgaaactg gctatagaaa
    14581 atgaaaaaaa caaaaaactc cgcacccgtt tcaactttgt gatggcacaa ctgcaggagc
    14641 ggacgggtga caaagttgcc gcatcggatt actataccga agttataaaa tcaaatcccg
    14701 gctatgaact caacttcaat gcacgcatca accgcgcaca actcctggcc cgaaatgtaa
    14761 aaagcataga gaaagaactg aaaaaaatgg cccgtaaccg caacaaccgc gattatctgg
    14821 accagattta tgctgctctg gggaatatgt atctgcagga aaaagatacc gtaaaagcta
    14881 tcagtaatta caatcttgcc cttgaaaaaa gtaaacgcaa cggaatagac aaagcggtgg
    14941 cagcactcaa actcggcgaa ctgtattaca accggagaga ctatgtaaaa gcccaaccgt
    15001 gctacgatga agcttccaaa atttttacca acgattatgc cgattatgcc agcatcagca
    15061 aacgggcaga aatgcttggt gaactggtca aagagcatga aataatagtg ctacaggaca
    15121 gcctgcaggc attggcgatg ttgagcgatg acaaacggct ggaagccatc aaccgggtga
    15181 tagaaaaggt aaaaaaagag gaaaaggaag aagctgaaaa taagaaaaat ccgcagcagg
    15241 gctcacgggg tatgaatgac cccgaagatg actttgccat gaatatgggt gcacccatcg
    15301 gcggtggaaa taacagaaac tggtatttct acaacccggt gaccatcaac tccggcaaag
    15361 cagattttaa gaaaaaatgg ggaaaccgca aactggagga caactggcgc cgcagcaata
    15421 aatcggctgc tctgtttgcc gacaacaccg gcagtactcc ggcaagctct gataacccac
    15481 tcactacagg aacggattcg gtgcagacac ccaaaataaa agccacttcg gataaaacaa
    15541 gcgtggatta ctacctgaaa caactgccgt ttaccgccga acaacgaaaa aaatcggatg
    15601 ctgaagtggc agatgccatg tttgcaaaag gatttatttt taaggataaa ctggaagatt
    15661 tccccatggc ctacagcact tttgaagaat ttcaacggcg cttcggcaac gacgaacgcg
    15721 tagtagaaac ccttttccag tgctacctca tggcttcgaa agagaaaaac gaatcccagg
    15781 ctgaagcata ccgaaaccgc atactcaccg cattccccaa cagtaaaatg gcacagctgc
    15841 tgaaagatcc ggactatatc cggcgtcagg gcagtatgtt tgccgaacag gattctcttt
    15901 attctcagac ttatgcagca tacacacgca gcgatttttc cacagtcttc aaaaatacgg
    15961 actattttcg taagaaatat ccgcttgctg ccttgctgcc aaaatttgaa tttctgaatg
    16021 cattgagcat cggaaaaaca gccgcttcaa ctgagtttga gaaatctctg agtaaattgg
    16081 ttgaatccta tcctcaaagc gatgtaagcg ccatgtcgaa ggacattctg gccctgatga
    16141 agcagggaaa cctggcgcag aaaggaacca gccacggcac gctgctgagc aagcgtgcag
    16201 ctgtcgacag caccaatatt gcccgcgacg cagccatgag tttctcgccc gacaaagagg
    16261 gaaaacaccg cttactgctg ctgaccgatg ccacatccga tgggataaat aaactgcttt
    16321 acaacctggc aatatttaat ttctccaggt ttatgattaa ggatttcgat tttgtggttg
    16381 gccccgccga tgccactcat cagggcatat cggtcaccaa cctcgaatcg tacgatgaag
    16441 tgctgtggta tcagaaaacg ctgaaaactg accagtcgct tacagccctg atggactcgc
    16501 tgaaagtaac acaggttgtc atttcggagg ataatttcgg aaaaatgaag tccattttca
    16561 gtctggacga atacctggca ttcgaaaaaa acaatctgat gaaagaaaaa ccggcaaatc
    16621 tggcagttag tacaacaagt actccgtcaa aaaaagaagt tgcaaaaaaa cctgcaacgg
    16681 aaaccctaca ggcatctccg gttaaagaag tcgccgtaac tgctgcttca acaacgcctt
    16741 cggcacaaaa aacaactaca ccatcagaac caaaaaccgt agccgccatt gaccagaaag
    16801 ccgcagcaca accggcaaaa aatattgaga cgactaaaac cgtgacgacc gataaaacag
    16861 ccacggcagt gaataaacag cctgctgcgt ctgaaccggt aaaaacagct gaaaaagcgg
    16921 aacctgaaaa acccgctgtg atggaagaaa aggtggaatg gtacaaaaat cttttcgcca
    16981 tccgtctcaa tgctccgcat tttgtagcat tttatcttcc acccggaaaa tttgattttc
    17041 cgaaaattca aaaagccctc gacgcttata atgcggccaa ctacagcacc atgaacctgg
    17101 cagtcaacct ggaagatccg ggtaaagaca aattcatcat agtaggaaca tttacagatg
    17161 ctaacgtggc aaaatcttac ttcatgcgta tgcttaaaga tccgggcatc acagaagcat
    17221 tcagaggggt aaacagacgc aatctggtag gaaccaaaga aaacctgaat atcatggtaa
    17281 aaaaagatgc tctcaatacc tattttgaat ttatgagaga atattacctg aaataaatac
    17341 taactttttt gttttttggt aatttgtgag gttgtttttc aagctccaaa ttaacaaaca
    17401 accacaacaa acaatcattt aaataaaaat atattccttc tgatgggaat aggggagagc
    17461 aaattatgaa aaaagacaga atactttggg cagacgatga aatggattta ctgaaaccct
    17521 acctgctctt tctggaagaa aaaggatatg aagtggtggc cgtcaccaac ggaaaagacg
    17581 ccattgagat atgccagaat gaaagttttg atatcatctt tctggatgaa aatatgcccg
    17641 gcctgagtgg cctcgaaacg ctggctcaga ttaaagtcct ggacccaaat gtaccgatgg
    17701 tgatgattac caaaagcgaa gaagaggaca tcatgaatat ggccatcggc aataaaatag
    17761 ccgattacct gaccaaacct gtgaatccaa gccaaattct gctgacgctg aaaaaaaata
    17821 ttcataaaaa ggaaatcgta tcagaacata ccagtaccag ctaccaacag caattcagaa
    17881 acattgggat gcagataaac gaatcactga cctacatgga ttgggtggat gtatataaaa
    17941 agctggttta ctgggagctg gaactcgcgg aaaccgaaaa tggcatggac gaaatgctga
    18001 aaatgcagaa aaacgaagcc aacagcacct tcaacaaatt tgtgaagaaa aactacctgg
    18061 actggatgag caatcctgca ctgcgaccat tgatgagcac cgatctcttc aaaacaaagg
    18121 tgtttccact ccttgataag ggtgaaaaag tcttttttgt gttggtggat aatttccgtt
    18181 acgaccagtg gattatcatc cgtgagttac taagtgaatt ttataccttc accgaagaag
    18241 aaacctattg cagtatcctg ccaacagcca cacaatatgc ccgaaatgcg attttctcgg
    18301 gactgatgcc acttcaaatt caacaaatgt atcctcatct ctggattgag gaggaagatg
    18361 aagagggaaa aaatatcaac gaggaagcgc tgctgcagac acaactggat cgtttccgca
    18421 aaaaatacaa tttctcctat cacaaggttt tcgaatcggg ttactgcgaa aaagtgatta
    18481 atcaacttcc ttccctggaa aataatcaac tgaacgtggt agtgctcaat tttatcgaca
    18541 tgctctcaca cgcacgcact gaaagcaaaa tgatgcgcga gttggccaac gacgaaaagg
    18601 cataccgatc gctgacgcta tcgtggttcc gccactccgc cacctacgat ttgttcaaag
    18661 ccattgcaca gcggggttac aaagtgattg tcacaaccga tcacggcacc attcaggtaa
    18721 acaatgctat caaagtagtg ggcgataaaa acgtaaatac caacctgcgc tacaaagtgg
    18781 gcaaaaacct gagttataac aagaaagaag tatttgaaat cctgcaacca cagaaagccg
    18841 gtctgccgag cccgaatgta agctctgcct atatttttgc cggaaatgac gatttctttg
    18901 cttatcccaa caacttcaac cactacgtaa cgtactataa aaacactttc cagcacggtg
    18961 gaatttcgct ggaagagatg attattccgg tagtgaccat ggagccgaaa taaaagtgcg
    19021 gccccctaac cctcccgaaa caagttcggg acaggctctg aagggggaat attaagagtg
    19081 catttcggaa aaggtttggg tttggcctca ttttgttaca aatctgtctg attaaatagc
    19141 agttacctct gagataactg atttttatct gatttcagaa aaacaaatcc caatcaatcc
    19201 atcaattcct accattattt tttgtaaatt tgcctattca atttcactaa ttaatttccc
    19261 cctttagggg gttagggggc cagcatgaaa acctacacaa acgtaaaaga cctcggcaac
    19321 ttgcaggagg ctgtaaaaga agcacttgaa ataaagaata accggtatgc ctacaagcag
    19381 ctgggtgaaa ataaaacact gctaatggtg ttttttaatt ccagcctgcg cacgcgactg
    19441 agtacccaaa aagccggaat gaacctggga atgaatacga tggtgctcga cattaatcag
    19501 ggtgcctgga agctggaaac cgagcggggc gtaattatgg acggtgaaaa gccggaacac
    19561 attctggaag ccattcctgt aatgggaacc tactgcgata ttatcggagt gagggctttt
    19621 gcacagtttg aaaacaagga atttgattat caggaaacca ttattaatca attcattcaa
    19681 tattccggaa agcctgtttt cagtatggaa gcagctacgg gtcatccctt gcaggctttt
    19741 gccgatttga ttaccattga agaatataaa aaaactgccc gccccaaagt agtactcacg
    19801 tgggcgccac acccaaaggc tttgccgcag gcagttccaa attcgtttgc cgactttatg
    19861 aacgaagccg atgtggaatt tgtcatcaca caccccatag ggtatgaatt gagtgaaaaa
    19921 tttgtgcgtg gagctcgcgt ggaatatgac cagatgaaag ctttcgaagg agcggacttt
    19981 atttatgcca aaaactgggc ggcttatcag gatccaaact acggaaaaat cctatcaact
    20041 gaccgcaact ggaccgtttc cgaccggcaa atggcggtta ccaacaatgc gtattttatg
    20101 cactgcctgc cggtgaggcg taatatgatt gtgaccgacg atgtaattga aagtcctcag
    20161 tccatcgtta tcccccaagc tgccaaccgc gaaatatccg cccaagttgt tattaaacga
    20221 ttattagaaa atatttaaaa aataaattat gaatttagaa caggttattg cctccatccg
    20281 caacgttccc gactttccga aaccgggaat tcagtttaag gatattacta ccgctatgaa
    20341 agatgcggaa gtgcttcatt atatttccga tacactgtac gaatactaca aggacaaagg
    20401 aataaccaaa gtagtaggag tggaaagccg cggttttatg ctcggaagca ttctggctta
    20461 taaactaaat gccggatttg tattgctgcg aaaaaaggga aaattaccgg ccgaaactta
    20521 cagcataaat tatgcactcg aatatg
//
LOCUS       DAOPZK010000020        20532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2685_length_20532_cov_26.168824, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000020 DAOPZK010000000
VERSION     DAOPZK010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20532
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2685_length_20532_cov_26.168824"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1422)
                     /gene="ftsZ"
                     /locus_tag="PKH68_02285"
     CDS             complement(97..1422)
                     /gene="ftsZ"
                     /locus_tag="PKH68_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNX11794.1"
                     /translation="MDYLEDTLPLDLPLANSSIIKVIGVGGGGGNAVNHMYKQGIADV
                     SFVVCNTDNQDLVRSPVPIKIQLGKNITQGLGAGGRPEVARRAAEESVADLHTMLADN
                     TKMVFITAGMGGGTGTGASPIVAKTAHDMGILTVGIVTIPFAFEGNVKIRQALEGVVA
                     LSEYVDAILVINNEKLKNIFPDLNLSNAFAKADDVLTNAAKAIAEIITISGYINIDFA
                     DVSTIMRDGKVAIMNTGYASGDNRITRAIDDALTSPLLNTSDVKGAKKVLLSLYCSHS
                     NEIKMEEITQIHDFMAKVGEDVTVIWGASFDDELGDDVKITLIATGFDVKDIPGMPSG
                     LVKKRKTTDERPENEPEHSEVPVSEEEMEEHKKSSEEEISKVMGELYGDSLRQNKTEK
                     REEVIQETIPFEAATDEKLPVVNILELESEEEFNRVEQIPTWKRRFMKK"
     gene            complement(1517..2791)
                     /gene="ftsA"
                     /locus_tag="PKH68_02290"
     CDS             complement(1517..2791)
                     /gene="ftsA"
                     /locus_tag="PKH68_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNX11795.1"
                     /translation="MSKNTIVHIDMGGSRISALAGVVLNDELKILGEQSRPSDDVKSG
                     IVEKMTGAAYKINETTKLLQNALRIEPIASVSITVNAKSMKQIPYTQKTTLRKPISEG
                     MLKKLKEECKEDLKNDKTDVIDCIPLAYYLDGKRIEDPVGQKGYNFRIDYNLIVCHPL
                     VIENIERSIERTGLAVDYIHLGMEAQATAVLEEDDRDNGCAILSFGSTTTSLAVYCEG
                     HLQELMVVPLGGYNITKDIEELGMSFANAETLKCKIGCALEELVVKPVNVQIPHVNPN
                     NDQIVISTKFLSTIIESRLDEILEPIFEKLEQVAFPLQTGIILTGGASKLKHLSDYIT
                     NRTGFPVRLGSHAEWLSADTDPKFKDPVYSQAVGAILLTDYLKKQKGEEEKVIKPRIP
                     SKNLITKATAIFKNKMTDLFDYDEMDRDQNDQ"
     gene            complement(2819..3556)
                     /locus_tag="PKH68_02295"
     CDS             complement(2819..3556)
                     /locus_tag="PKH68_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11796.1"
                     /translation="MNKILKYILLTAGVVICLAYAVYGAYSGISTGKEQVCSNIEVNI
                     QDFNNRQLITNTEIARLLETEGLNPIGKTMKRIKTKSIEDAIEKHPMVRKAECFKTPV
                     GEITVSIEQRIPVLRVVGYENYYVDDQRKPMPVSQNFAAYVPVISGRVTQKMATGVLF
                     DFADYINKDPFWSNQIMQININDKLKVELVPRVGDHMVMMGDLSRYKQKLEKLKMLYL
                     YGLNEIGWNQYKTIDLQFRDQVVCTKK"
     gene            complement(3596..4969)
                     /gene="murC"
                     /locus_tag="PKH68_02300"
     CDS             complement(3596..4969)
                     /gene="murC"
                     /locus_tag="PKH68_02300"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNX11797.1"
                     /translation="MAFTQYYFIGIGGIGMSAIARYYHVKGFAVAGYDRSETKLTSDL
                     QAEGISITFEEGVASIPPDFRMKEKTLVVITPAVPQEHEQLVYFREHNFKILKRAEML
                     GEITRQSKAICIAGTHGKTTTSTITAHLLYQSHVNCSAFLGGISNNYNTNLLLSNESN
                     YVVVEADEYDRSFHQLTPFMAVIISVDADHLDIYGTKEAFLESFEHFTSLIMPGGALV
                     VNKKAELKPKLKKGVKMFTYGLDPTADFHAKNIRTEKGNILFDFVTPTDTIYDVRLGV
                     PIMINVENSVAAMAMAWLNGVTNEELRTGISTFSGIYRRFNIVFNSEKVVYMDDYAHH
                     PSELKESISSIRQLYPERKITGIFQPHLYSRTRDFAPEFAKSLSQLDEVILLPIYPAR
                     ELPIEGVTSELILNDVTSIHKELSSKENLLTLLDEKELDVIVTFGAGDIDKLVPEIKN
                     YLKKRFS"
     gene            complement(4972..6078)
                     /gene="murG"
                     /locus_tag="PKH68_02305"
     CDS             complement(4972..6078)
                     /gene="murG"
                     /locus_tag="PKH68_02305"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNX11798.1"
                     /translation="MKYIISGGGTGGHIFPAISIANAIKKQDPFAEILFVGAIGRMEM
                     ERVPQAGYPIEGLPVRGFDRKNLLKNVSVIIDLLKSTRKAKKIIAAFKPDIAIGVGGY
                     ASAPTLKAASNVGVPTLLQEQNSYAGVTNKLLAARASKICVAYEGMEKFFPAEKIILT
                     GNPVRQDLFAVAPKAREAYEFFGFDPAKKTILIVGGSLGARTINRSIKEGLQSLAGSD
                     VQCIWQTGKLYVTEAREAAASFRNSNMIVTDFVTRMDYAYAIADLVISRAGASSISEL
                     CLLAKPVILVPSPNVAEDHQTKNALALVNRDAAVMIRDADAEKSLVHKALELIENNPA
                     LEQLSKNIAQLAQRNSADRIVDEVFKIIEQRKKF"
     gene            complement(6183..7430)
                     /locus_tag="PKH68_02310"
     CDS             complement(6183..7430)
                     /locus_tag="PKH68_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNX11799.1"
                     /translation="MENFFKKYLKGDTIVWTVFFILCIFSIIELYSASSTLAFKAASH
                     TAPIVQHFSYLALGAVLAYVVHLVPYRYIRIFAYLGLLISILLLIYVLLKGQDTNDAS
                     RWIKLFGIQFQPSEPAKLSLIVVIADFISRIRANPADENRNFWIIMAIAGVVCGLIFL
                     ENFSTAAILGLTIYFMMFIGRISWKKLASILAVGVILLVIGYGMVKAIPEEKMPDMFH
                     RAYTWVARIDRSAGENDKDKYVINDENLQVQHGRIAVARGGLFGVFPGNSVQRDFLPH
                     AFDDFIFSIIIEEMGLLGAIFIMFLYMVLLFRTGQIATVCKSTFPAMLVTGLGLLITL
                     QATVNMFVGSGFGLVTGQPLPLISRGGTSIMINGIYFGIILGVIREIKAEKEKKIKQK
                     EQVSQPVEEAEEEEYVLDIEEIG"
     gene            complement(7503..>8129)
                     /locus_tag="PKH68_02315"
     CDS             complement(7503..>8129)
                     /locus_tag="PKH68_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNX11800.1"
                     /translation="LQREEKTKAFIENEQLIINTLNNVFTMPATELSLKGMHNTYNSM
                     AAGLTASIVNIRKESIRQSLQEFQGVEHRLEYVATVKNVKYINDSKATNVNSCWYALQ
                     SMKTQVILILGGTDKGNDYTEIEELVKEKVSGLIFLGVDNSKLHEFFDGKVENIADAH
                     SMEECVKTAYMMAKPGETVLLSPCCASFDLFQNYEDRGKQFKSCVRNL"
     assembly_gap    8130..8229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8230..8663)
                     /locus_tag="PKH68_02320"
     CDS             complement(<8230..8663)
                     /locus_tag="PKH68_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNX11801.1"
                     /translation="MKKIVVLGAGESGAGAAVLARKQGFDVFVSDMGEIKPEYKQMLN
                     DHGIGWEEKKHTEELILNADEVIKSPGIPDKAPIIQKLKAQHTPVISEIEFAGRYTDA
                     KMICITGSNGKTTTTMLIYHILKNGGLNVGLAGNVGKSLALQV"
     gene            complement(8679..9944)
                     /gene="mraY"
                     /locus_tag="PKH68_02325"
     CDS             complement(8679..9944)
                     /gene="mraY"
                     /locus_tag="PKH68_02325"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNX11802.1"
                     /translation="MLYHLIHYLDKYYDIPGSGMFLSISFRTGLAFILALLVSTMIGR
                     KIIDRLQLLQIGETIRDLGMEDQLQKKGTPTMGGIIIIVSILIPTLLLADLTNIYTLL
                     MIITCVWMGAIGFLDDYLKIKYRNKEGLKGRFKIVGQIGLGLIVGLTLYLSPDAVIRE
                     NAEIKGENARVENVLYASHDVKSTKTTIPFMKNNNLDYADAFKWAGDQAQKYGWILFV
                     LMAIFIVTAVSNSANLTDGMDGLATGSTAIIGVTLGILAYVSGNVNYAGYLNIMYLPG
                     TGELLIFAGSLIGATVGFLWYNSYPAQVFMGDTGSLTLGGIIATFSIAIHKELLIPVL
                     CGLFFVESISVIVQRFYFKYTKKRFGQGKRIFLMTPLHHHFQKGGKSGIAAAINKPWN
                     PVHESKLVVRFWIIGIILAAISIITLKIR"
     gene            complement(10037..11482)
                     /locus_tag="PKH68_02330"
     CDS             complement(10037..11482)
                     /locus_tag="PKH68_02330"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNX11803.1"
                     /translation="MNLTDLLQNIITEKITGKSDIEISGIQFDSRIVAAGNLFVATRG
                     TASDGHQFIPMAIQKGATAILCEQLPQELNPEVTYIQVKDSADALGLVASCWYGFPSS
                     KLILVGVTGTNGKTTIATLLYKLFRELGYDAGLLSTVCNYVNDDAVEATHTTPDALEL
                     NALLARMVEAGCKYAFMEVSSHAVEQRRIAGLEFDGAVFTNLTRDHIDYHLTFENYLK
                     AKKRFFDDLDGNAFALTNADDKNGMVMLQNSRAKKFSYSLRTMADFKTKILDDGFEGM
                     LLDMNNREVHVSFIGKFNASNLTAVYGAAVLLGQDELEVLRIISSLKSVSGRFDTLYS
                     PTGYAAIVDYAHTPDALNNVISTINEILKGNGRLITVVGCGGNRDKGKRPMMAREAVD
                     GSWKAILTSDNPRNEEPMDILNDMLAGLDPSQKAKSLTIADRREAIKTACSLAQKGDV
                     VLVAGKGHEDYQIIQGVKHHFDDKEEVRKCF"
     gene            complement(11508..>12713)
                     /locus_tag="PKH68_02335"
     CDS             complement(11508..>12713)
                     /locus_tag="PKH68_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNX11804.1"
                     /translation="VSLMAVLDDGKAKLTDVIATGNGLYSYGRAVMKDHNARKGGYGD
                     ITLEQSLNASSNVGISRTIVKAYGDNPAAFVNKLYKMKLNEPFNLYIPGSAAPNIRHP
                     NDKSSYWSQSTLPWMSIGYEVQIPPIYTIAFYNAIANNGKYLEPLIVKSISKNGQIVK
                     QFTSRTINERICKPSTLKDVRQALLGVVENPKYGTGKAVHSQYVRIAGKTGTAQISKG
                     SQGYKSGGTSHQVSFCGFFPFENPKFTCLVVIREPKIGYPSGGLMAGVVVKNIAERIS
                     ALDTVANVRAMPVDSNYIDAKNPVIKSGKQAPLQTVLAELNQKYSGKNSEWVKTLSDG
                     KTTQVESLAITNKTVPDLSGMGAKDAVFLCERLGLTVNFSGIGKIVSQSLAPGTAVHK
                     GQQITLTLK"
     assembly_gap    12714..12813
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12814..13025)
                     /locus_tag="PKH68_02340"
     CDS             complement(<12814..13025)
                     /locus_tag="PKH68_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11805.1"
                     /translation="MSDPGKNIISRFAAVYVFIVLLLLLIIYNIVVIQTVERKEWLAL
                     GSKSKRTDIIVLPNRGNIYSADGRLMA"
     gene            complement(13038..13517)
                     /locus_tag="PKH68_02345"
     CDS             complement(13038..13517)
                     /locus_tag="PKH68_02345"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNX11806.1"
                     /translation="MSWFKKILETIKGSEDFSDIKSSTLRDILNGSILKKKFIQKHYG
                     LFILIAFLIFVYIDNRFYCETQISKEVKLKQQLKDIKFESLTISAQLTTLSRRTYVMN
                     YINEKGLNLKESPTAPAIITEPDPEKDEEVLKAKKEQQDASNKIKNDTTVVEEYIIN"
     gene            complement(13514..14428)
                     /gene="rsmH"
                     /locus_tag="PKH68_02350"
     CDS             complement(13514..14428)
                     /gene="rsmH"
                     /locus_tag="PKH68_02350"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNX11807.1"
                     /translation="MSDTPYHIPALLSQTMDGLNINPDGTYVDVTFGGGGHSREILSH
                     LSSKGKLIGFDQDEDALKNAIDDPRFIFVRSNFRYLTNFLKYHNVEQVDGILADLGVS
                     FHHFDESDRGFSFRFEGALDMRMNTKSPVTAASIINTYEEEKLANVFYLYGELHNSRR
                     IASTIVKARQSQPIALIQEFVDILKPHFSREKEKKDMARVFQALRIEVNKEMIVLQEL
                     LTQSVNVLKPGGRLVVLTYHSLEDRLVKNFFRSGNFEGELEKDFYGNVLAPLKPVNTK
                     VIVASDEEVARNPRARSAKLRIAEKTRI"
     gene            complement(14429..14887)
                     /locus_tag="PKH68_02355"
     CDS             complement(14429..14887)
                     /locus_tag="PKH68_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNX11808.1"
                     /translation="MSTFIGKYDAKADVKGRIFIPSSYRKLLPEGDRSKVVMRKDADN
                     DCLVLYPESVWNKKLDDFKSKLDEWNADDQLLLMQFVSDAEWLEIDSQGRVLISKKYL
                     QLIGIENSEVLFVGMMDRFAVWGKSKFESVKLTPVDFAARLKEKMTKQPE"
     gene            15278..16102
                     /locus_tag="PKH68_02360"
     CDS             15278..16102
                     /locus_tag="PKH68_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX11809.1"
                     /translation="MENGKVMQLNVEQIIKEKAPGAKFPKFVVNYLKRIIHQEELNTF
                     FESVPGQKNLEFIESALTDHLQAGADFEGIEHLPPKEGRYIFVSNHPLGGLDGVTLGL
                     MFGRKYDGKVKIFANDILMFLEPLKEMFLPVNKVGAGSNSRDNAKIVDDFFKSDYHLI
                     TFPAGTCSRRIGGKIQDLPWKKSFVTKAVQYRRDVIPVYFEGRNSNFFYNLSKIRTLL
                     GLPNIEMLYLVNEMYKQRGNRFKVKIGQPIPYQTFDNTKTPVEWAAEVREKVYALK"
     gene            complement(16143..16661)
                     /gene="rimM"
                     /locus_tag="PKH68_02365"
     CDS             complement(16143..16661)
                     /gene="rimM"
                     /locus_tag="PKH68_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445025.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNX11810.1"
                     /translation="MIAKNEIFPIGRLLKTHGLSGEMTFSTTTTLLDEIEIPYLILEP
                     EGIAVPFFIEEVRMKSGETGLIKFEGIDSGEQAREYVGLTLYLPNEYLEEMDETDEEA
                     GYFVGFEVVEENHGKIGTITDVDQSTENILFVIETDDDEILIPAVEEYITDIDHEKKI
                     IRLRLPDGLLDL"
     gene            complement(16761..16937)
                     /locus_tag="PKH68_02370"
     CDS             complement(16761..16937)
                     /locus_tag="PKH68_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX11811.1"
                     /translation="METVEKVLEAIKQAGQPVNAGKVVELTGLDRKEVDKAMTKLKAT
                     GDIVSPKKCFWTAK"
     gene            complement(16999..17397)
                     /locus_tag="PKH68_02375"
     CDS             complement(16999..17397)
                     /locus_tag="PKH68_02375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNX11812.1"
                     /translation="MTIFSRIIAGEIPCYKVAEDENFFAFLDINPMSKGHTLVVPRKT
                     ENDYLFNLDDETYTGLTLFARKVAKAIEKSVDCKRIGVAVMGLEVPHTHIHLIPINKE
                     GDMNIGNPKLKLAPEEMAEIAEKIRNNFNQ"
     gene            complement(17439..17909)
                     /gene="greA"
                     /locus_tag="PKH68_02380"
     CDS             complement(17439..17909)
                     /gene="greA"
                     /locus_tag="PKH68_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNX11813.1"
                     /translation="MAVTYMTADGYKKLVDDLNEMENIQRPAISKLIAEARDKGDLSE
                     NAEYDAAKEAQGMLEMKIAQLKDILGSARMIDETKISTHEVQILTRVKIKNVKSGQVM
                     TYLIVSESEANLKEGKLSVTTPIAKGLLGKKVGEKASVNVPSGAMEFEVMEITL"
     gene            complement(18067..18549)
                     /locus_tag="PKH68_02385"
     CDS             complement(18067..18549)
                     /locus_tag="PKH68_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11814.1"
                     /translation="MEDLGDYIYLILLVLAGMSGIFSKKKKESNTQEKKKPVINLPKS
                     WEEFEQQTTTRSQKPVVKAQTTQREFKPERTIMDEGVSGEIIPGEFETGTFLTYDSVD
                     DTSKMRAKKTVTKSFKSKPDYSLPDIAEQEMNTTFTLDNPESARSAFIYSEIFNRKYN
                     "
     gene            19000..20373
                     /gene="miaB"
                     /locus_tag="PKH68_02390"
     CDS             19000..20373
                     /gene="miaB"
                     /locus_tag="PKH68_02390"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNX11815.1"
                     /translation="MNETNSVASPDSKSALNDKKLFIETYGCQMNSADSEVVASIMQM
                     DGYELTENISEADAIFVNTCSIRDNAEQRVINRLKNFQSLKRKKKNLVIGVIGCMAER
                     VKEDLIENHGVNLVVGPDAYLDIPNLMGHAMNGEKAINVELSKTETYADVMPSRISNP
                     ISGYISIMRGCNNFCSYCIVPYTRGRERSRDVESILNELRDLQAKNFKEVTLLGQNVN
                     SYSFEKDGQKIEFPDLLRMVAEAAPEIRFRFTTSHPKDMSDRTLEVIAAHPNLCRFIH
                     LPVQSGSNKILKLMNRKYTREWYLDRIAAIRRILPDATIGTDVFSGFHSETDEDHQET
                     LSLMREVGFDMAFMFKYSERPGTHAATHLPDNVPEEVKLRRLDEIIKLQNELSNASNQ
                     RDVGKDFEVLVEGFSKRSKEQLFGRTSQNKVAIFPREGRRIGETIRVKITRASSATLF
                     GEVVEKV"
BASE COUNT         5276 a         4525 c         4273 g         6258 t
ORIGIN      
        1 tcagcgctta tctggtcaaa taatttttct tccatgttgg ttttaatgtt tataattatt
       61 gatattcagc agtttatctg ctacaataat tgttggttat tttttcataa atcggcgttt
      121 ccaggtcgga atttgttcca cgcggttgaa ttcttcttcg ctttcgagtt ctaggatatt
      181 aaccaccggc aatttttcat cggttgccgc ctcaaaaggt atagtttcct gaattacttc
      241 ttcgcgtttt tccgttttat tctgccggag cgaatctccg taaagctcac ccataacctt
      301 ggatatttct tcttcactgg attttttatg ctcttccatt tcttcttccg ataccggtac
      361 ttcgctgtgt tcgggttcgt tttcaggccg ttcgtccgtg gtttttcttt ttttgaccaa
      421 tcctgatggc attcccggta tgtctttcac atcaaacccg gtggcaatga gtgttatttt
      481 tacatcatcg cccagttcgt cgtcaaaact tgctccccag ataacggtaa catcttcgcc
      541 cactttggcc atgaaatcat ggatttgggt gatttcttcc atcttaatct cattggaatg
      601 agagcaatac aggctcagaa gtactttttt cgctcctttt acatcactgg tgttaagcag
      661 cggagaggtc agcgcatcat caatggctcg tgtaatacgg ttatcgcccg aggcataacc
      721 ggtattcatg atggccacct taccgtcgcg catgatggtg cttacatcgg caaagtcaat
      781 attgatgtat cccgaaatgg taatgatttc ggcaatagcc tttgctgcat tggtgagcac
      841 atcatcggct ttggcaaatg cattcgacag atttaaatcg ggaaaaatat ttttaagttt
      901 ctcgttgttt atcaccagta tggcgtcaac atattcgctc agagcgacca ctccttcgag
      961 tgcctggcga attttcacat taccctcgaa agcaaaaggt atggtaacaa ttcccaccgt
     1021 gagtattccc atgtcgtgtg ccgttttggc tacaatgggc gatgcaccgg tacctgttcc
     1081 accacccatg ccggcggtga taaataccat tttggtgtta tcagccaaca ttgtatgcaa
     1141 gtcagccacc gattcttcgg ctgcacgccg ggctacttcg gggcgtccac cggcaccaag
     1201 tccctgtgtg atgttttttc caagctgaat tttaatagga accggagagc gaaccaaatc
     1261 ctgattatcg gtattgcaca ctacgaatga gacatcggca attccctgtt tgtacatgtg
     1321 attgactgca ttgccgccgc ctccacctac gcctataact tttatgatgg acgaattagc
     1381 cagtggaagg tccaatggca gggtatcttc taaataatcc ataatttatt gttgttttaa
     1441 agtcagaata ttgtggtaac aatgaaatgc attatgctga cctgttggtt tctaatttcg
     1501 ttgaaaaata aaaaatttat tgatcatttt gatcccgatc catttcatcg taatcaaaaa
     1561 gatcggtcat tttattttta aaaatagctg ttgcttttgt aattaaattt tttgaaggaa
     1621 ttcttggttt tataaccttt tcttcttcgc ctttctgttt tttcagataa tcggtcagca
     1681 gaatcgcccc cacagcctga gagtaaaccg ggtccttgaa tttcggatcc gtatcggccg
     1741 acagccattc ggcatgactg cccaaacgaa ccggaaagcc ggttctgttg gtgatataat
     1801 cgctcagatg tttcagtttt gaagcaccac cggtaagtat gatgccagtc tgcagcggaa
     1861 atgcgacctg ctccagtttc tcaaaaatag gttcaagtat ttcgtcgagt cgtgattcga
     1921 tgatagttga cagaaatttg gtggaaatca cgatttgatc attattggga ttgacatgcg
     1981 gaatctgaac attcacgggt ttgaccacca gttcctcgag tgcacagcca attttacatt
     2041 tcagtgtttc ggcatttgca aaactcatac ccagttcttc aatgtctttg gttatgttgt
     2101 agccacccag cggaactacc atcagttcct gcagatgccc ttcgcaatac acagccagtg
     2161 atgtggtggt ggagccaaat gaaagtatgg cgcaaccatt atcccggtcg tcttcttcga
     2221 gtacggctgt agcctgggcc tccataccca gatgaatata atccacagca agtcctgttc
     2281 gctcgatact tcgttctatg ttttcgatta ccagtgggtg acaaacaatc aggttatagt
     2341 caattcgaaa attatatccc ttttggccta caggatcttc tatccgcttt ccatccaggt
     2401 agtaagccag tggaatgcag tcaatcacat cggttttgtc atttttcaaa tcttctttgc
     2461 actcttcttt tagctttttg agcattcctt cacttatcgg ttttcgcagt gtggttttct
     2521 gggtgtaggg aatttgcttc attgatttgg cattcaccgt gatactgaca ctggcaatag
     2581 gctcaatacg taaggcattt tgcagcagct tggtcgtctc attgatttta taggctgcac
     2641 cggtcatttt ttccacaatt ccacttttca cgtcatcgct cgggcgtgac tgttccccca
     2701 gaatttttag ttcatcgttc agcaccacac ctgccagtgc cgatattcgt gaacctccca
     2761 tatcaatgtg tacgattgtg tttttgctca taaggaataa ttttctacag gaattaattt
     2821 actttttggt acataccacc tggtctctga actgcagatc aatggttttg tactgattcc
     2881 atccgatttc gttcaacccg taaagataca acattttcag tttttccagc ttttgtttgt
     2941 aacggcttaa gtcgcccatc atcaccatat gatcgcccac ccggggtaca agttccactt
     3001 ttagcttgtc gttgatgttt atttgcatta tctgattgct ccagaagggg tctttgttga
     3061 tgtaatcggc aaagtcgaac aaaactcccg tagccatttt ctgtgttacc ctgccggaaa
     3121 ttaccggaac ataagccgca aaattttgcg ataccggcat cggttttcgc tgatcgtcca
     3181 cgtaataatt ttcatagccc acaacccgga gtaccggaat acgctgttcg atgctcacag
     3241 ttatttcacc tacgggagtt ttgaagcatt cagccttcct caccatcgga tgtttttcaa
     3301 tggcatcttc aatcgatttc gtttttattc gcttcatggt ttttcctatc ggatttaatc
     3361 cttcggtctc cagaagtctg gctatctcgg tgttggtaat cagttgtcgg ttattaaaat
     3421 cctgaatatt tacctcaata tttgaacaga cctgttcttt accggtcgaa atgccactgt
     3481 aggcaccata aaccgcatac gccaggcaaa taacaacgcc tgctgtaagt aatatgtatt
     3541 tcagtatctt gttcattcag tgtgattttt gtttttatgt tccaataaaa aattactatg
     3601 aaaatctttt cttcagataa ttttttattt caggaacgag tttgtcgata tcacctgcac
     3661 caaaggtaac tatcacatcc agctcttttt catccaacaa agttagcaaa ttttccttgg
     3721 atgataattc tttatgaatt gatgtgacat cattcagaat aagttcggat gtaacaccct
     3781 caataggcaa ttcgcgtgca gggtagatgg gcagaagtat cacttcatcc agctggctca
     3841 gcgatttggc aaattccggt gcaaaatcgc gggtgcggct gtacaggtgc ggctggaaaa
     3901 ttccggtaat tttacgctcc gggtaaagct ggcgaatgga cgaaatactc tctttcagtt
     3961 cgctcggatg atgggcgtaa tcgtccatat ataccacttt ttccgaattg aaaacaatat
     4021 tgaaccgacg gtaaatgcct gaaaaggtag agataccggt tcggagttct tcattggtta
     4081 ccccgttgag ccaggccata gccatggctg ccacgctgtt ttccacattt atcatgatgg
     4141 gtaccccaag gcggacatca tatatagtat cggtaggagt cacaaaatcg aatagaatat
     4201 tccctttttc agttcgaatg ttcttggcat gaaagtcggc tgttgggtct aatccgtagg
     4261 taaacatttt tacccctttc ttcagttttg gcttcagttc cgctttttta ttgaccacca
     4321 aagctcctcc gggcatgatt agtgaggtga aatgctcgaa actttcgaga aatgcctctt
     4381 tcgtaccata aatatccagg tggtcggcat ccacagagat tatcacagcc ataaacggcg
     4441 tcagctggtg aaaggaccgg tcgtactcat cggcttccac taccacgtaa ttactctcgt
     4501 tcgaaagcag cagattggtg ttgtaattat tggaaatacc gcccagaaaa gcgctgcaat
     4561 tcacgtgcga ctgatacagc aggtgtgctg tgatagtcga agtggtggtt ttgccgtggg
     4621 ttccggctat gcagatggct ttactctgtc gggtgatttc gcctagcatt tcggcacgtt
     4681 tcagtatttt gaaattatgt tcgcgaaaat aaaccagttg ctcgtgttcc tgcggtaccg
     4741 ccggagttat caccaccaat gttttttctt tcatccgaaa atcaggtgga atgctggcca
     4801 caccttcttc gaatgtaatg ctgatgccct cagcctgcag gtcggatgtg agttttgttt
     4861 cggagcggtc gtaaccggcc acagcaaaac ccttcacatg ataataccgc gcaatggcgc
     4921 tcatgcctat tccacctatt cctatgaaat aatattgtgt aaaagccatt gtcaaaattt
     4981 ttttctttgt tcaataatct taaaaacttc atcgacgatt ctgtcagccg aatttcgttg
     5041 tgcaagttgg gcgatgtttt tcgagagctg ttccagtgcc ggattatttt caatcagttc
     5101 cagcgcttta tgaacaagac ttttttcggc atcggcgtca cgtatcatca cggctgcgtc
     5161 ccggttgacc agtgcgagcg catttttggt ctgatggtct tcggccacat tgggtgaagg
     5221 caccagtata acgggcttgg caagcaggca aagttctgaa atggaactgg caccggcacg
     5281 tgaaattacc aaatcggcta tggcgtaagc ataatccatc cgggttacga aatccgtcac
     5341 tatcatatta ctgttccgaa aggatgcagc agcctcgcgt gcttcggtaa cgtacagttt
     5401 tccggtctgc cagatacact gaacgtccga accggcgagt gattgcagcc cctcttttat
     5461 gcttcggttg atggttctgg cacccagact gccaccgact attaatattg ttttttttgc
     5521 cggatcaaaa ccaaaaaact cataagcttc ccgggcttta ggagcaactg cgaataaatc
     5581 ctgccgaaca ggattgcctg taagaattat tttttcggcc ggaaaaaatt tttccattcc
     5641 ttcgtaagcc acgcatattt tactggcacg ggctgcgagc agtttattgg ttactcctgc
     5701 atatgagttt tgttcctgca gcagggtcgg cactccaaca ttggaagcag ccttcagggt
     5761 gggagcactg gcatatccgc ccacacctat ggcaatgtcg ggcttgaaag ctgcaataat
     5821 tttttttgct ttgcgggtgc tttttagcag gtcaataatt acactcacgt ttttcagcag
     5881 gtttttgcgg tcgaaaccgc gtacaggcaa tccttcgatt ggatatccgg cctgcggtac
     5941 ccgttccatc tccatacgac cgatagcccc tacgaataga atttcagcaa aaggatcctg
     6001 ctttttgatg gcattggcta tgctgatagc cggaaaaatg tgaccgcctg tgccacctcc
     6061 gctgatgatg tatttcataa ttattcctcc ttaattttaa ttgcctgacc gggaattgtc
     6121 tgttaaaagt tacatgttaa atattccaca aacttaataa ttgggaattc atgagcaaat
     6181 aattatccaa tttcctcaat atcaagtacg tattcttcct cctcggcttc ctcaaccggc
     6241 tgactgacct gttctttttg ttttatcttc ttttcctttt ccgcttttat ttcacgtatt
     6301 actcccagaa ttattccgaa gtagataccg ttgatcataa tggaagttcc gccacggctt
     6361 attaaaggca acggctgacc ggttaccaaa ccaaatccgg aacccacaaa catattgacc
     6421 gtggcctgca gcgtgataag caagcctaaa ccggtaacaa gcatggccgg aaaggtactt
     6481 ttgcatacag tggctatctg gccggtcctg aagagtaata ccatgtagag aaacatgata
     6541 aaaattgcac ccaataagcc catttcttca ataatgatgg agaagataaa atcgtcaaaa
     6601 gcgtgtggga gaaaatcccg ttgaacgctg ttgccgggaa aaactccaaa caaaccaccg
     6661 cgggctacgg caatacgtcc gtgctgaacc tgaagatttt cgtcattgat cacatattta
     6721 tccttatcat tttcgccggc cgaacggtcg atgcgggcta cccaggtgta agcacggtga
     6781 aacatatcgg gcatcttctc ttcaggaatg gctttaacca ttccataacc tataacaagc
     6841 aatataaccc ccacggccag aattgaagcc agttttttcc acgatatccg gccaataaac
     6901 atcatgaagt agatagtaag accgagaatg gctgccgtag agaaattttc cagaaagatc
     6961 aaaccacata ccacaccggc gatggccatt atgatccaga aatttcggtt ttcatctgcc
     7021 ggattggccc gtatgcgtga tatgaaatcg gcaataacga cgatcaggga caattttgca
     7081 ggctcagagg gttgaaactg aatgccaaac agctttatcc accggctggc atcgttggtg
     7141 tcctgaccct tgagcaacac ataaataagc agcagaatag aaatcagaag tcccaggtaa
     7201 gcaaaaatgc gtatgtaccg gtatggaacc agatgcacca cataagccaa aactgctccc
     7261 aatgccaggt aggaaaaatg ctgaacaatg ggtgcggtat gactggctgc tttaaatgcc
     7321 agcgtactgg aggcactgta caattcaatg atagaaaata tacacaggat aaagaatacc
     7381 gtccagacga tggtatctcc ttttaggtat ttcttaaaaa aattttccac ttcttgtttt
     7441 gttcaatcgg gtgaataaat aggttaaaaa taaacgctta atatgggcac tgtgaaaaat
     7501 gtttacaaat ttctgacaca ggatttgaat tgttttccac gatcctcgta attctgaaat
     7561 aagtcgaaac tggcacagca gggtgacagc aggacggttt cgccgggttt tgccatcatg
     7621 taagcggtct tcacacattc ttccatggag tgcgcatcgg caatattttc aacttttcca
     7681 tcaaagaatt catgaagttt ggagttatca actcccaaaa aaattaaccc gctcactttt
     7741 tcttttacca gttcttctat ttcggtgtag tcgtttcctt tgtcggttcc gcccaggatc
     7801 aggattacct gagttttcat gctctgaagg gcataccagc acgaatttac gttggtagct
     7861 ttggagtcat tgatgtattt tacatttttc actgtggcta catactccag tcggtgctct
     7921 acgccctgaa attcctgcaa cgattgccgg atactttctt tgcgtatatt gactatggaa
     7981 gccgtaagtc ctgctgccat tgagttgtag gtgttgtgca ttcccttgag tgatagttcg
     8041 gttgccggca tggtaaaaac attatttaaa gtattgataa tcaattgttc gttttcgata
     8101 aaagcttttg ttttttcttc gcgctgcagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnna cctgcagtgc caggcttttt cccacattgc cggccagacc cacattgagt
     8281 ccgccatttt tcagaatatg gtatatcagc atggtagtcg ttgttttgcc attgctcccg
     8341 gtgatacata tcattttggc atcggtgtat cttcctgcaa attcaatttc ggaaataacg
     8401 ggtgtgtgct gtgctttcag tttttgaatt attggcgcct tgtcgggtat tccgggactt
     8461 tttataactt catcagcatt taatatcagt tcttcggtgt gttttttctc ttcccacccg
     8521 atgccgtgat cattcagcat ttgtttgtat tccggtttta tctcgcccat gtccgataca
     8581 aatacatcaa agccctgctt tctggccaat acggctgcac cagcaccact ttcacctgct
     8641 ccgagtacta ctattttttt cattttttat tgaactgatt atcttatttt caatgtaata
     8701 atgctgatgg ctgccagaat gatgccgata atccagaatc ttactaccag ttttgattca
     8761 tgtaccggat tccagggttt attgatggcg gctgcaatgc ccgattttcc acctttctga
     8821 aaatgatgat ggagtggtgt catcagaaaa attcgttttc cctggccaaa tcgttttttt
     8881 gtgtatttga aataaaagcg ttgaacgatt actgagatac tttccacaaa aaataatccg
     8941 cacaatacgg gtatcaacag ttctttgtga atggcaatgg agaaggtggc gataattcca
     9001 cctaacgtca gactacctgt atcgcccata aacacctgag ccggatagga attgtaccac
     9061 aaaaatccga ccgtggcacc aatgagtgaa ccggcaaaaa tgagcagttc acccgtacca
     9121 ggcagataca tgatattgag gtatccggca tagtttacat tacccgacac ataggccagg
     9181 ataccgagcg tgacaccaat aatagctgtg gaaccggtgg caagtccatc catcccgtct
     9241 gtcagattgg cactgttcga tacggcagtt acaataaata tggccatcag cacaaataaa
     9301 atccaaccgt atttctgggc ctgatcaccc gcccatttga aggcatcggc gtaatccagg
     9361 ttgttatttt tcatgaaggg aatggtcgtt ttggtcgatt ttacatcgtg cgaagcatac
     9421 aaaacatttt ctacccgtgc attttctcct tttatttcgg cattttcacg gattacggca
     9481 tccgggctga gataaagtgt aagtcctaca ataaggccaa gtccaatctg acctactatt
     9541 ttaaatcttc ctttgagtcc ttccttgttc cggtatttta ttttcagata atcgtccaga
     9601 aatccgatag ctcccatcca cacacaggtg ataatcatca gcagggtgta aatattcgtc
     9661 agatcagcaa gtaacaaagt aggtattagt atcgaaacga tgatgataat tcctcccatg
     9721 gtaggtgttc cctttttctg aagctgatct tccatgccca gatcacggat ggtttcacct
     9781 atctgcagca attgcaaccg atctattatt tttcgtccaa tcatggttga taccagcaaa
     9841 gcaagtataa aggctaagcc ggtgcgaaac gaaatggaca ggaacatgcc cgatccgggt
     9901 atgtcgtagt atttgtccag atagtggatt aagtgataca gcataaactt aaattttttg
     9961 tgttgctatt tataagtcat gttcctaatc tgagaataag gatttttgaa tcgtgtctgt
    10021 gataaattta acgttttcaa aaacattttc tcacctcttc tttgtcatca aaatgatgtt
    10081 ttacaccctg aatgatctga taatcctcgt gtccttttcc tgctaccagc accacatcgc
    10141 ccttttgggc taaagaacag gctgttttta tagcttcgcg gcggtctgca atggttaaac
    10201 tttttgcttt ctgcgatggg tccagtccgg cgagcatgtc gttcagaatg tccatgggtt
    10261 cttcgttgcg cggattatca gaggtaagta ttgctttcca gctgccatcg acggcttcac
    10321 gtgccatcat cgggcgtttc cccttgtcac gattgccgcc gcatcccacc acggttatca
    10381 gccggccatt gcccttcagt atttcattaa tcgtcgaaat cacattgttc agcgcatcgg
    10441 gcgtatgagc ataatccact atggcagcat atccggtggg cgaatacagc gtgtcgaacc
    10501 ggcctgatac tgatttcagc gaactgatga tgcgaagtac ttccagttca tcctgaccga
    10561 gcaatacagc agcgccgtaa actgcggtaa gattggatgc attgaatttt ccgataaagg
    10621 aaacatgcac ttcgcgattg ttcatgtcca gcagcatacc ttcaaatccg tcgtccagaa
    10681 tttttgtttt gaaatcggcc atggtgcgta gcgagtaact gaactttttt gcccgggaat
    10741 tctggagcat caccattccg tttttatcgt cggcattggt gagtgcaaat gcattgccgt
    10801 cgagatcatc aaaaaagcgt ttttttgctt tcagataatt ttcaaatgtc aggtgataat
    10861 cgatgtggtc gcgcgtcaga ttggtgaaaa ccgcaccatc aaattccaga ccggcgatgc
    10921 gtctttgctc cactgcgtgt gaactcactt ccataaatgc atatttgcag ccggcttcca
    10981 ccatcctggc aagcagcgca ttgagttcaa gcgcgtcggg ggtggtgtga gtagcttcta
    11041 ccgcatcatc attcacataa ttgcaaacag tggaaagcaa tcctgcatca tagccgagtt
    11101 cgcggaaaag tttgtacagt agtgtggcaa tagtcgtttt cccgtttgtt cccgtaactc
    11161 caaccaaaat caattttgac gaaggaaatc cataccagca cgatgccacc agtcccagcg
    11221 catcggcgct gtcttttacc tgaatataag taacttcggg attcaactct tgtggcaatt
    11281 gttcacaaag aatggctgtt gccccttttt gaatggccat gggaataaat tggtgtccat
    11341 cggaagcggt accgcgtgta gccacaaata aattgccggc cgcaactatg cgggagtcga
    11401 actgaatgcc ggaaatttca atgtctgatt ttccggtaat tttttcagta ataatatttt
    11461 gtaataaatc ggttaaattc atccctgcca gattttaatt ggttgattta ttttaatgtc
    11521 agtgtaattt gttgcccctt gtgaactgct gtgcccggtg ccaaactctg agaaacgatt
    11581 ttgccaattc ccgaaaaatt taccgtaagt cccaatcgtt cacaaagaaa aacagcatct
    11641 tttgcaccca taccgctcag gtcgggaact gttttatttg ttatggcgag tgattccacc
    11701 tgtgtagttt tgccgtcgct tagtgttttg acccactcgc tgttttttcc ggagtatttc
    11761 tggtttaatt cagccaatac ggtctgcagc ggggcttgtt tgcctgattt tatcaccgga
    11821 ttttttgcat ctatataatt actgtccacc ggcattgctc tgacattggc caccgtatcg
    11881 agagccgaaa tccgctcggc aatgtttttt accactacac ccgccatcag tccgcccgag
    11941 ggataaccga tttttggttc tctgattact accaggcagg taaatttagg attttcgaaa
    12001 gggaaaaatc cgcagaagga aacctgatgt gatgttccac cggatttata accctgtgac
    12061 ccttttgaaa tctgagctgt acccgttttg ccggcaattc gcacatactg tgaatgaaca
    12121 gctttacctg ttccgtattt tggattttcc accacaccga gtaaagcctg ccgcacatcc
    12181 ttcagtgtcg atggtttaca gattcgctca ttgatggtgc gcgaggtaaa ttgcttgaca
    12241 atctggccgt ttttgctgat ggattttaca ataagcggtt ccaggtactt accattattg
    12301 gctatagcgt tgtaaaatgc aatggtataa ataggaggaa tctgcacttc gtaaccgata
    12361 gacatccagg gtaatgtgct ctgtgaccag taactgcttt tgtcgtttgg atgccggata
    12421 ttgggtgctg ccgaacccgg aatgtaaaga ttgaaaggct cgttcagctt cattttatac
    12481 agtttgttga caaatgcagc cggattgtcg ccatatgcct tcactattgt tctggaaatc
    12541 cccacattcg acgaagcatt caatgattgc tccagggtaa tatccccata gccgcctttt
    12601 cgggcattat gatctttcat tacagctcgt ccataagaat acagaccgtt gccggtggct
    12661 atcacatcgg tcagtttggc ttttccgtcg tccaatacag ccatcagcga tacnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccatga ggcggccgtc ggctgaatag
    12841 atattgccac gattaggcag tacgatgatg tcggttcttt tactttttga tccgagtgcc
    12901 aaccactctt tgcgctctac agtctgaatt acgacaatat tatatatgat aagcagaagc
    12961 agcaatacga taaatacgta tactgctgca aatctgctga ttatattttt gccgggatca
    13021 gacattgtaa tgattgctta attaattata tattcttcaa cgactgtggt gtcgtttttt
    13081 attttatttg aggcatcctg ttgctctttt ttggctttca gcacttcctc atctttttcc
    13141 ggatcgggtt ctgtgattat tgccggtgcg gtaggacttt ctttcaggtt caatcctttt
    13201 tcgttgatat aattcattac atacgtacgg cggcttaggg tggtgagctg agcggagata
    13261 gtgagcgatt cgaatttgat gtccttcagt tgctgtttca gtttcacttc tttggatatc
    13321 tgagtttcgc agtagaaccg gttgtcaata tatacgaaaa tcagaaatgc aatcaggata
    13381 aaaagtccgt aatgtttttg aatgaatttt tttttcaata tgctgccgtt cagaatatcg
    13441 cgcagggtac tcgatttgat atccgagaaa tcttcgctac ctttaatggt ttctaatatt
    13501 tttttgaacc agctcatatt ctagtttttt ctgcaattct aagtttagcg ctgcgggcac
    13561 gtggattgcg ggctacttct tcgtccgaag ccactattac ctttgtgttt acgggcttca
    13621 ggggtgccag tacattcccg taaaaatctt tttcgagttc accttcaaaa tttccgctgc
    13681 ggaaaaaatt cttcaccaaa cggtcttcca gcgaatgata agtgagcact accagcctgc
    13741 cacccggctt gagcacattt acgctttgtg tcagcagttc ctgcagtact atcatttctt
    13801 tattcacttc aatgcgcaat gcctgaaata cgcgtgccat atcttttttt tctttttctc
    13861 tcgaaaaatg gggtttcaga atatccacaa attcctgaat aagtgcaatg ggctgcgact
    13921 gccgggcttt gacgatggtg gatgctattc ttctggaatt gtgcaattca ccatacagat
    13981 aaaacacatt cgccagcttt tcttcctcgt aggtgttgat gatgcttgcg gctgtaaccg
    14041 gtgattttgt attcattcgc atgtcaagcg ccccttcgaa ccggaatgaa aatccgcggt
    14101 ccgattcgtc gaaatgatgg aatgaaacgc ccagatcggc gagtattcca tcgacttgct
    14161 ccacgttgtg gtatttcaga aaattagtca gatatcgaaa attgcttctt acaaaaataa
    14221 atcgcggatc gtcgatggca tttttcaggg catcttcgtc ctggtcaaat ccaatgagtt
    14281 tacccttgct gctgaggtgc gagagaattt cacgcgaatg tccgccacct ccaaaagtga
    14341 catcgacata cgtgccgtcg gggttaatgt tcagcccgtc catcgtttgg ctcaataatg
    14401 ccggtatgtg ataaggtgtg tctgacattt attcgggttg tttggtcatt ttttccttta
    14461 gccgggctgc aaagtcaacc ggtgtaagtt ttacgctttc aaattttgat tttccccaca
    14521 cggcaaagcg gtccatcatt cctacaaaaa gaacttcact gttctcaatt ccaataagtt
    14581 gcaggtattt tttggagatc agcacccgac cctgcgagtc tatttccagc cattcggcat
    14641 ccgacacaaa ctgcataagc agcaactggt catctgcgtt ccattcgtcc agttttgatt
    14701 tgaaatcatc cagtttttta ttccagacag actcgggata aagcaccagg caatcattat
    14761 cggcatcctt gcgcatcacc accttgctgc ggtcgccttc gggcaatagt ttgcggtaag
    14821 aggacggaat gaagattctt ccctttacat cggcctttgc gtcatatttg cctataaatg
    14881 ttgacataac tgtttgaatt ataatatcga atggtaaaag tatataatat ttttcaaatt
    14941 ccccacattt ccccacaaaa tatttatcaa caagttataa accgatttta cccgattttt
    15001 acagagttat caacatgttt tcagttcggt ttatatcatt taaaaacaac taagaagcat
    15061 aatatcaatg tattatgaat ttaataacga gaaatggcgt ggaagtgggg agagcagaat
    15121 aacttcaaga taaccgtgac caaattttta ttcataatac agtgtcaaaa ggtgatgcaa
    15181 tggtaattga tttcgggcgt atcggcagat aaaatttcgg taagcaggat ttttttataa
    15241 ttttgtgaaa taaaagaatt taatcagaag cagcggtatg gaaaacggaa aagtaatgca
    15301 actgaatgtg gaacaaatta ttaaggagaa agctcctggc gcaaagtttc caaaatttgt
    15361 ggtgaattac cttaaaagaa taattcatca ggaagagtta aatacatttt ttgagtcggt
    15421 tcccggtcag aaaaatctgg aatttattga atctgcttta accgatcatt tgcaggccgg
    15481 tgccgatttc gagggaattg aacacttgcc gccgaaagag ggaaggtata tttttgtgag
    15541 caatcatccg ctgggagggc tcgacggtgt cacactgggt ttaatgtttg gccggaaata
    15601 cgacggtaag gtgaaaattt ttgccaacga tatcctgatg tttcttgaac cgttgaaaga
    15661 gatgttttta ccggtaaata aggtgggtgc cgggtccaat agcagagaca atgccaaaat
    15721 agtggacgat tttttcaaat cggattatca tcttattact tttccggccg gtacctgttc
    15781 gcgccgaata ggcggaaaaa ttcaggacct gccctggaaa aaatcatttg taaccaaggc
    15841 tgtgcagtat cggcgcgatg tgattcctgt ttattttgaa ggcagaaatt ccaatttctt
    15901 ttacaacctc tccaaaatca gaaccctgct gggcttgcca aatattgaaa tgctatatct
    15961 ggtgaatgaa atgtacaagc aacgcggaaa ccggtttaag gtcaaaatcg gacaaccgat
    16021 tccctatcag actttcgaca acaccaaaac accggtggag tgggcggctg aggtacggga
    16081 aaaagtctat gccctgaaat gagcattgca gaaatccatc attcgtactg ctgctgaggt
    16141 acttacaaat caagcagccc gtcgggtaag cggaggcgga ttattttttt ttcgtggtca
    16201 atgtcggtaa tgtactcttc cacagcaggt atgagtattt catcatcgtc agtttcaatc
    16261 acaaacagaa tattttctgt ggactgatcc acatcagtaa tggttccaat ttttccgtga
    16321 ttttcttcca ctacttcaaa tccaacaaaa taacccgctt cttcgtcggt ttcatccatc
    16381 tcttcgagat attcattggg cagatacagg gtaagtccta catattcgcg cgcctgctcg
    16441 ccggagtcaa taccttcgaa cttaatcaga ccggtttcac cggatttcat cctcacctct
    16501 tcaatgaaaa atggcacggc aatgccttcg ggctccagta tgaggtaggg gatttctatc
    16561 tcatccagta gtgttgtggt ggtactgaag gtcatttccc cgctcaatcc gtgcgttttc
    16621 agtagtcgtc cgatgggaaa aatttcgttt ttggctatca tattcgtata tttaaaatac
    16681 ataagcacca agtgtgcacg ttaccacgtc ttgtgtctaa cggggttgga tgtgtactta
    16741 gtgcttgtgt actaaatgaa ttattttgct gtccagaagc atttttttgg tgacacaata
    16801 tcacctgttg ctttcagttt ggtcatcgct ttatccacct ctttgcggtc aagtccggtg
    16861 agttccacta cttttccggc attaaccggc tgacctgcct gcttgatagc ttctaatact
    16921 ttttctacag tttccatagt ttgaatgatt tttgagtgat tattttgaaa atattacagg
    16981 tactatcgtt ctactatttt attggttaaa attatttctg attttttcag caatttcagc
    17041 catttcttcg ggtgccagtt tcagttttgg gttgccgatg ttcatgtcac cttccttgtt
    17101 gatggggatt aggtgaatat gggtatgagg aacttcaagt cccattacag ccactccgat
    17161 gcgtttgcag tccaccgatt tttcgatggc ttttgccacc ttacgggcaa acaaagtcag
    17221 gccggtgtac gtttcatcgt ccagattgaa caaataatcg ttttcggttt tcctgggcac
    17281 taccagtgta tgacccttgc tcatgggatt aatgtccaga aaagcgaaaa agttttcgtc
    17341 ttcggctact ttataacaag gaatttcgcc ggctatgatt ctgctgaaaa tggtcatagt
    17401 ttctgtctga taaattttgt ttcctgtttc taaaaaattt ataatgtaat ttccattact
    17461 tcaaattcca tagcacctga aggtacattc accgaggctt tttcacccac ttttttacct
    17521 aacaatcctt tggcaatggg tgtggtaacc gacagtttac cttccttgag gttggcttcg
    17581 ctttccgaaa ctattaaata ggtcatcacc tgacccgatt tgacattttt aatttttacg
    17641 cgggtcagta tctgcacttc gtgagtcgaa atctttgttt catcaatcat gcgcgccgat
    17701 ccgagaatgt ctttgagctg tgcaattttc atctccagca ttccctgcgc ttctttggct
    17761 gcgtcatatt ctgcattctc cgacaaatct cctttatcac gggcttctgc tataagtttg
    17821 gatatagcgg ggcgctgaat gttttccatt tcgttaagat catccaccaa ttttttataa
    17881 ccatcagcgg tcatataggt cactgccata atgtttgttg ttaagtttta gtttatttgt
    17941 aaacaaaaaa gaattccgac aaacagtcgg aactctcagt agaaaatgtg attatatatg
    18001 caaagataca cgcttttttc gaaattcaat ttttttttga aaaaaagttt tttttgaaaa
    18061 aaggatctaa ttgtatttcc ggttgaaaat ttcggagtaa ataaatgccg accgggcgct
    18121 ctcgggatta tccagtgtaa aagtggtatt catctcttgt tcagcaatat ccggcaatga
    18181 ataatccggt ttcgatttga aacttttcgt tacggttttt tttgcccgca ttttgctggt
    18241 atcatccacc gagtcgtaag taagaaacgt gccggtttcg aattcccctg gaattatctc
    18301 acccgacacg ccctcatcca ttatggttcg ttccggcttg aattcccgtt gagtagtttg
    18361 tgcctttacc accggttttt ggctccgggt agtggtttgt tgctcaaact cctcccacga
    18421 tttggggagg ttgatcaccg gttttttctt ttcctgagta ttgctttcct ttttcttttt
    18481 gctgaaaatg ccactcatgc cagccagtac gagcaaaatc agataaatat agtcgcctaa
    18541 gtcttccata cagatatatt attgaatgca atattacaaa ataaaatgat tactgacaac
    18601 catcaacaca aaaaaaacaa tgggtaactt cacagccacc cattgctaca ttttaaccta
    18661 aaccttaatt atgaaaaatt tatctgtttg tttactctgc aaatgtagta cgtttttttt
    18721 aattaccaaa taaatttcat tcttttttta aaatatttta ttagataatg ctattaaatg
    18781 aacaaaaatg gaagaagttg accgttagta actgataatc tttaatttgt ataataatat
    18841 tggagaaaaa taccggccgg ctaatacaca tatgtgcagc gaacttacac ttctgacaaa
    18901 aaatagtaac tttgcacccg gaaacttttt tgtcaatcac agatacgctg tgagagatta
    18961 gtattaattg cttgaagcaa atacataaat attaagctaa tgaacgaaac aaattcagtc
    19021 gcttcgccgg actctaaatc tgcgctgaac gacaaaaaac tctttatcga aacctacgga
    19081 tgtcagatga actcagctga cagcgaggtg gtggcatcca tcatgcagat ggatggatac
    19141 gagctgacgg aaaatatttc ggaagccgat gccatttttg taaatacctg ctctattcgc
    19201 gataatgccg agcagcgtgt cattaaccgg ctgaaaaact ttcagtcgct gaaaaggaag
    19261 aaaaaaaatc tggtgattgg cgtcatcggc tgtatggccg agcgggtcaa agaagatttg
    19321 attgaaaatc acggcgtaaa tctggtagtc ggccccgatg cctacctgga tatccccaac
    19381 ctgatgggac acgccatgaa tggcgaaaag gctatcaatg tggaactctc caaaactgaa
    19441 acttacgccg atgtgatgcc ttcgcgcatc agcaatccca tttccggata tatttccatc
    19501 atgcgcggat gcaacaattt ttgctcctac tgcattgtgc cctacacgcg tggccgtgag
    19561 cgcagccgcg atgtggaaag tattctgaat gaactccgcg acctgcaggc aaaaaacttc
    19621 aaagaagtga cgctgctggg gcaaaatgtt aattcctact ctttcgaaaa agacggacag
    19681 aaaatcgaat ttcccgattt gctccggatg gtggctgagg ctgcacccga aatccgcttc
    19741 cggtttacca cctcacaccc caaggatatg agcgaccgca cactggaagt gattgcagcc
    19801 catcccaacc tgtgccggtt tatacacctg ccggtgcaga gcggcagcaa caaaattctg
    19861 aaactgatga accgcaaata cacccgtgaa tggtatctgg accgcattgc agctatccgg
    19921 cgcattttac ccgatgccac tatcggcacc gatgtgttca gcggtttcca cagcgaaacg
    19981 gatgaagatc atcaggaaac attgtcattg atgcgcgagg tgggttttga tatggccttt
    20041 atgttcaagt actccgaacg tcccggcaca cacgcagcga cccacctgcc cgacaatgtg
    20101 cccgaagagg tgaagctccg gcggctcgac gaaattatta agctgcaaaa tgaactttct
    20161 aacgccagca accagcgcga tgtaggaaag gattttgaag tcctggtcga aggtttctca
    20221 aaacgttcca aagagcagct tttcggtcgc acttcgcaga ataaagtggc gatttttccg
    20281 cgcgaaggac gccgaatcgg cgaaactata cgcgtgaaaa taacaagagc cagctcggca
    20341 acccttttcg gtgaagtggt agaaaaggtg taaaataatt tttaaccgat gacccgtttt
    20401 ttaagaatat gtaaaatact ctgaaaaaca acgtttaacc tgacgtttca aactgtaagg
    20461 tttgtcgttg aaaaaatact gaatttcaaa gaaagcagaa cgtaaagttt tgctttttta
    20521 tttgctgaaa at
//
LOCUS       DAOPZK010000021        20312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2732_length_20312_cov_23.380264, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000021 DAOPZK010000000
VERSION     DAOPZK010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20312
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2732_length_20312_cov_23.380264"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKH68_02395"
     CDS             <1..573
                     /locus_tag="PKH68_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNX11816.1"
                     /translation="LNVEKAMQHKVLDSEEIRNIYTALGVTIGTEDDSKELNLSKLRY
                     HKVIIMTDADVDGSHIATLLMTFFFRHMKEIVEQGYLYIATPPLYRCYKGKIEEYCWT
                     EQQRQAFIEKYGGGSEAGIHTQRYKGLGEMNEIQLWDTTMNPDNRTLRQVTIENAATA
                     DHVFSMLMGDDVAPRRQFIEQNATYANIDA"
     gene            801..1349
                     /locus_tag="PKH68_02400"
     CDS             801..1349
                     /locus_tag="PKH68_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNX11817.1"
                     /translation="MKVCVFCSSSNNIPEKYKQSAYRFGQFLANEGITLVYGGATGGL
                     MDAVAQGAKAGDGEIIGVIPDIIIKMNRLSHLPTQQIIVSTMSERKMRLKQEADAFVV
                     LPGGYGTLDEMFDVIASGNVGEHRKPLICVNEDGFYRQLLGQIATMRTECFTPKNENY
                     KPVFVATLEECMKQIRILHTNK"
     gene            1356..2675
                     /locus_tag="PKH68_02405"
     CDS             1356..2675
                     /locus_tag="PKH68_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX11818.1"
                     /translation="MNLFWKKRFGGMLSTSKFEKMEEAAVSDMKRYDEIAKSLELEEY
                     RKLFHVVKSSEFKEKKKTLQNRKYQDTEEYRISRKYQKLHTNSSIRQYYETLHSERLN
                     TFLDFKTTPEYEELGDKKKVKASEKLQKLKDFENSKEYKNYIRYHDSFIIRDYEELKK
                     KIATPEFVKSNQFWADQHRWKTVPEHAVEQRYYELAKNPDIIFYENEKPARFEKYRKL
                     SPAFIEDFDWYTLDKSRWAYGFHASNEQLIGNYSFDNERQANSGGKNVSVVHGTFTLE
                     TKHEKCISRAWNPTKGFYEKEFDYTSDVVQTADEFRQTYGIFKAKIRCTGRAHHAFWL
                     SNNSKLPHINIFHYDGKAITMGNAHENVMDEVRITGLKHSSFYIYTLEWTPKELIWYI
                     NDLEVHRTTSNVPKENLYLGFNSFLPAKEKPSTGKLEVDWVKVLSFN"
     gene            2745..3629
                     /locus_tag="PKH68_02410"
     CDS             2745..3629
                     /locus_tag="PKH68_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNX11819.1"
                     /translation="MEKPKIIAEIGCNHKGEMDIALEMIKTAATFCHVDVVKFQKRNN
                     HELLTEEEFNAPHPNPYNSYGATYGEHREYLEFSLEQHRQLQSWCNEFGVEYSCSVWD
                     VTSAKEIISLNPAIIKIPSACNLNQELLTCICDHFDGEIHVSFGMTDREEEEKIVSFF
                     EKKGRNKELVIYSCTSGYPVPFEDICLLELTRLRKTYESRVKAIGFSGHHLGIAVDAA
                     AVALGAEWVERHFTLDRTWKGTDHAASLEPDGLRKLCRDCRAVYKALKYKEEDILEIE
                     KPQLEKLKRNQIRWNDDK"
     gene            3611..>4410
                     /locus_tag="PKH68_02415"
     CDS             3611..>4410
                     /locus_tag="PKH68_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HNX11820.1"
                     /translation="MERRQVTAFIPVRGGSKSIPLKNIREFCGKPLVYWNIAALQAAG
                     LVDKIVVATDSDDIEATVRSFGFDKVEIYRRSAENASDTASTESVMLEYIEAANLSEE
                     TVFMLVQATSPLTQTIHFQEALQLYSEGNYDSLLTCVVNKRFFWNKNGTPRNYDYMNR
                     PRRQNFDGELMENGAFYINTVKNILQSGNRLSGKIGIYEMPEYTAIEIDEPDDWTMLE
                     NLMRKHVLKTQPVTKIKLFLTDVDGVLTDGGMYYSEIGDEQKKFNTRDG"
     assembly_gap    4411..4510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4511..5718)
                     /locus_tag="PKH68_02420"
     CDS             complement(<4511..5718)
                     /locus_tag="PKH68_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNX11821.1"
                     /translation="MWWMDLGVKTVIMGAKIASLWNKKIKQGLDGRRHLAESLAKDIK
                     PSDRVVWFHAASFGEFEEGRPLIEEIRSRYPNYRILVTFFSPSGYNAHKNYPLADRVC
                     YLPADTTRDVRAFLDVVHPEIAVFIKYEFWPNLLAELKNRSVRTFVVSARFIPNSRFF
                     RWYGGVFRKALSTFEVLFVQDSRSIELLKTIGLTNAILAGDPRFDRVLSISKEEWKDE
                     IVEKFRQDGKLFIAGSTVGAGDEDLLQKLINKHPETRFLLVPHEMDREPMNKIVANTT
                     GEVLIYSECTPDTDFTNTQVLIVDKIGMLARLYRYGNWAYIGGGFVAGIHSVIEATIY
                     GMPAVFGPNYNKNRPGLEMVELGICASVSNIEELDAWFTPLEFDDEKLQKISQQARDY
                     SLKNCGATEI"
     gene            5823..6659
                     /locus_tag="PKH68_02425"
     CDS             5823..6659
                     /locus_tag="PKH68_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNX11822.1"
                     /translation="MRFKKIYIEITNSCNYHCSFCFDTSRPKKFMSPEEFSLVLSEIR
                     PFTDYIYLHVLGEPLLHPNIGRILQLSAEAGLKVNITTNGSLLQKQKELLLQHPVRQI
                     NISLHDAEENIQPEKWKEFVQTMLNSATDLSKNTYINFRLWNQTSKSSKEFNDLCFSL
                     IKDTFQLEEKHDLSAQSGRNITLAPNIWLQLAPRFEWIKNETTQVKNCYGLRDQIAIL
                     SDGSVVPCCIDADAHLLLGNIFTGKLTDILQSERAQRIKNGFEQHKAIEPYCQSCGFR
                     AE"
     gene            6903..7361
                     /locus_tag="PKH68_02430"
     CDS             6903..7361
                     /locus_tag="PKH68_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HNX11823.1"
                     /translation="MKTTAICPISDKRINENVARGNATLTVLFLTAFAITSNVLLIVF
                     LLIDFLLRGMELSRYSPFAIICSKVVKLLGIKPKIINAGPKLFAARIGVFFSLAILVS
                     SLSGLEITAYILTGVFGVCAFLEAFFGYCVACKIYPFVYRTFYAASCQQS"
     gene            complement(7489..8646)
                     /locus_tag="PKH68_02435"
     CDS             complement(7489..8646)
                     /locus_tag="PKH68_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX11824.1"
                     /translation="MEEYNFDDIRSYNTDDIPAAMERLCSQKPFMKVVSTLFPLMPKE
                     QLKQRLLSFTTSNELQQQMVYPFLQYLEANMTTGIVLHGLENIDRDQKYLYISNHRDI
                     ILDSALLCAKLIENKLDTVEIAIGDNLLVFPWIEDLVRVNKSFIVQRGLNARQVLESS
                     KRLSAYLRHAMKERKNSIWIAQREGRAKDSDDRTQESLLKMFNLGGNSGSFIDNLKEL
                     NICPLSISYQYDPCDFLKAKEFQLKRDNPDYKKSPEDDLINMQTGVMGYKGKVVYHIS
                     GCINGELDAIARETPNRNEQIERAAHLIDYHIHRNFSITAENKIAYDEIHGDKDFAKD
                     YSTEEKTTFEEYLDKQIRKIDLENKDRDFLRYKMLEMYANPLKNYMKANNI"
     gene            complement(8696..9505)
                     /locus_tag="PKH68_02440"
     CDS             complement(8696..9505)
                     /locus_tag="PKH68_02440"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNX11825.1"
                     /translation="MNNFVKRSISGGIFVAITISSILLSPYTFAAVFALFTAMAAYEF
                     HKLTNKPAEVQVNKTVAVIGAVLLFICSFLYASGRFQYPIYSFYGLFIVGVFIAELYR
                     KKQNPVNNLAYFILGQLFIALPFSLLSFILYVSDYQPIILLAVFITIWVNDTGAYVSG
                     MLLGKHKLFERISPKKTWEGFIGGAVFALISGYVFSLFIPELNLLQWFIFSEIIVIFG
                     TFGDLTESLIKRTENVKDTGNIIPGHGGLLDRFDSMLMSAPVIFLWLSFVF"
     gene            complement(9561..10223)
                     /locus_tag="PKH68_02445"
     CDS             complement(9561..10223)
                     /locus_tag="PKH68_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNX11826.1"
                     /translation="MNNNSSRDKILKRISEAKQTREFGSVFSVSPDDEIYKPIEPNAV
                     TCFKTELEAVNGQCELSETQDELFVQLKKILNDRNIDTIFCRDRQIGTHLTKHGIAFT
                     GCQDDFETMSAAVTPCEFLIARTGSVVVSSAGESGRQLLSYPPVHIVLAGTNQLVAYP
                     ADGYDALQKKYAEHLPSQITTITGPSRTSDIEKTLVLGAHGPKEFIVLLCNDKSFNNF
                     AL"
     gene            complement(10213..12279)
                     /gene="ftsH"
                     /locus_tag="PKH68_02450"
     CDS             complement(10213..12279)
                     /gene="ftsH"
                     /locus_tag="PKH68_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNX11827.1"
                     /translation="MENKNPTPKPLKTGGKPSKFNFTWIYALIMIGLLASYLIGNDTP
                     SKQVNVTQYEQYLNKGYIEKVYIYSNNTLQAFIIKDSASYVFGKDSTYNKKAPAISLL
                     IGSPEEAKKTIDNASKTLGREIQVEYKTNKDYLNLILWGFGPIILMVFLFWMMNRRMG
                     GMGGGAGGGIFSVGKSKAQLFDKGDGQNKITFKDVAGLQGAKQEVEEIVEFLKQPTKY
                     TELGGKIPKGALLVGPPGTGKTLLAKAVAGEADVPFFSMSGSDFVEMFVGVGASRVRD
                     LFRQAKEKAPCIIFIDEIDAVGRARGKNPNMGSNDERESTLNQLLTEMDGFGSNSGVI
                     ILAATNRADILDKALLRAGRFDRQIHVDLPDVHERKEIFNVHLRPIKIDNTVDVDFLA
                     RQTPGFSGADIANVCNEAALIAARKAKSFVGKQDFLDAVDRIIGGLEKKNKITTVEEK
                     KSIAYHEAGHATISWKLRYANPLVKVTIVPRGEALGAAWYLPEERQLTNREHMLDEMC
                     ATLGGRAAEEVFLTQTSTGAINDLERVTKRAYAMVAYYGMSEKLPNMSYYDSTGNSDY
                     GFTKPYSEKTAETIDEEARLIVAEQYERAKQILKENAAGHNTLAEILLDQEVIFAEDV
                     ERIFGKRPWESRSDELMRLNEDANALREKAKEAEQKAELPSSDETDGGEEKTGTADTN
                     QEKNEQ"
     gene            complement(12431..12790)
                     /gene="rsfS"
                     /locus_tag="PKH68_02455"
     CDS             complement(12431..12790)
                     /gene="rsfS"
                     /locus_tag="PKH68_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNX11828.1"
                     /translation="MIENEVIVEKIVEGIQEKKGKNIVVVNLNKLNDAPCSYFVICQG
                     DSNTQVNAIGTSIKDWVLEKTNIKPYATDGFENCQWIAMDYGQIIVHVFQRPFREFYD
                     LEHLWEDADLNFIPDLD"
     gene            complement(12863..13603)
                     /locus_tag="PKH68_02460"
     CDS             complement(12863..13603)
                     /locus_tag="PKH68_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11829.1"
                     /translation="MKRLIQLVAMFLLLSISTFAQQAVTQSVITLKTGERYRGEIVMR
                     TDEIVMLKTGDGKRYQFRVGEIEKIKQETLREIQVDANGNRTAGSHFAGIISVNGGAA
                     SAPGAVSLSPSAHLSLALGTRNAFHSNAFLGFGLGVETIFEKNNNLNYLPVFIQIQSD
                     RSKQIAGGIKTGYDFALNKIYKGGPMAEISGGVNQQLTGNTGLFFGLFAQVRQINGMV
                     TETTPWGKFTGTNNAALYTLGLKAAYMF"
     gene            complement(13607..14734)
                     /locus_tag="PKH68_02465"
     CDS             complement(13607..14734)
                     /locus_tag="PKH68_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX11830.1"
                     /translation="MKPFDVYPLFDIEITKGRGCYTYDKNGVEYLDMYGGHAVISIGH
                     SHPYYIQKMEEQMEKLVFYSNSVQNSLQVKLAEKLGKLSGYEDYSLFLINSGAEANEN
                     ALKLASFHTGRKKVVAFSKSFHGRTSAAVRVTDNPNIVAPLNEDLDVEFLPLNDIQAV
                     KEALKNKDVCAVIIEGIQGVGGIRVPEPEFLKSLREVCTATGTILILDEIQSGYGRSG
                     KFFAHQHAGIRPDMITTAKGMGNGFPIGGVLISPLFKPVHGMLGTTFGGNHLACSAAI
                     AVLDVMKGEKLVENAATVGSYLIKKLNAFPQIKEIRGMGLMIGIEMNEPVKQLRNNLL
                     FEQKVFTGASGTHVIRLLPPLNISKQDADLFLSRFSNALNS"
     gene            complement(14903..16960)
                     /gene="htpG"
                     /locus_tag="PKH68_02470"
     CDS             complement(14903..16960)
                     /gene="htpG"
                     /locus_tag="PKH68_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNX11831.1"
                     /translation="MAKGNIGVTSDNIFPVIKKFLYSDHEIFLREIISNAVDATQKLK
                     TLAAVGEFKGELGDLTIRVSVDPKKKKLTVSDRGIGMTAEEIDKYINQIAFSGAEEFL
                     EKYKNDAQNIIGHFGLGFYSSFMVSKKVEVFTQSFREGATGQHWSCNGDPEYTLEETI
                     KADRGTDIVMHIDDDNLDYLEESKIQELLTKYCKFLPVEIAFGKQKEWKDGKQVETDK
                     DNIINDVNPAWVRKPADLKDEDYDKFYHELFPMSDEPLFHIHLNVDYPFNLTGILYFP
                     KIKNNIDIQRNKIQLFCNQVYVTDHVENIVPEYLTLLHGVIDSPDIPLNVSRSYLQSD
                     ANVKKISSHITKKVADRLNDIFKTDRAQFEQKWDDLKLFIQYGMLSDEKFYERAEKFA
                     FLKNVEGKYFTFEEYKNLVEGNQKDKDGDLIYLYATNKDEQYTHLEAARAKGYDVLLM
                     DGQLDVHAISQLEQKLEKTRFVRVDSDVVEKLIRKEDTGKVELTDEQRDALITIFDSQ
                     LPSIEKTNFIVTFEKLSPEANPVFITQNEFMRRMKEMAAMQNGGMMNFYGEMPDSYNL
                     VINTAHPLIEKLQKEEQETTEAEVLPFTAQLSELKNRQTSLKDSHKDKKEDEIPTAEK
                     EELDSLQQKIDELTEGKKTVYKNFASGNKAVGQLIDLALLANNMLKGEALAKFVKRSI
                     EIL"
     gene            17171..18709
                     /gene="zwf"
                     /locus_tag="PKH68_02475"
     CDS             17171..18709
                     /gene="zwf"
                     /locus_tag="PKH68_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNX11832.1"
                     /translation="MNERIKTKAKNSTEGQVLVIFGANGDLSHRKLLPAIFHLFVDNL
                     LPDKFAVIGAGSDEKTEETFRTEVRTQLAQFAPETVADNPDKLTKFLELLYYKPVNNK
                     IKEDFDGLKSYINTLCTRLDIPRNLIYYFSIPPFMYEVVAAHLVSSGLNTEKDGWKRI
                     IVEKPFGYSYDSAVELDGKLHDGFAEHQIYRIDHYLGKETVQNIMVTRFANGFFEPIW
                     NRRYVDRVEITSAEKIGVGNRGGYYDTSGAMRDMIQNHLLQVLAIVAMEPPAVFESES
                     IRNESVKVLQALRPINPNEVEKYVIRGQYLQSATDGELMQGYRQEKGVAPGSKTETYV
                     GMKVFVDNWRWGDVPFYIRTGKRLPSRVTEVVIHLKPAPLQLFKHRCISQSTNMIILR
                     IHPDAGIAVDFGMKIPGAGYKVQNVNMDFHYSDLAQNKIPEAYERLLLDCMIGDSTLY
                     ARADAVRASWKFVDPILTAWKNNPDIPLYFYESNTWGPKEANQLFDNKLLKWLPPSCK
                     FNCE"
     gene            18711..19442
                     /gene="pgl"
                     /locus_tag="PKH68_02480"
     CDS             18711..19442
                     /gene="pgl"
                     /locus_tag="PKH68_02480"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNX11833.1"
                     /translation="MTENNMYTFETTEEVARAVARVIAVNTNRQERNKRYYNVALSGG
                     NTPKLLFQILASEFQEKIRWQALRLFWVDERCVPPSDHESNYGMTYENLLKNIYIPVT
                     NIFRMKGENDPSEEAVRYQALLEKELKLKKGIPQFDLVLLGMGDDGHTASIFPDNLAL
                     LHADETVAVARHPVSGQMRITLTGKIIRQARQRMFLITGKSKSNVLNEILKNEVPAQK
                     YPAFHILSLPDTEVYLDREAASFLG"
     gene            19483..>20312
                     /locus_tag="PKH68_02485"
     CDS             19483..>20312
                     /locus_tag="PKH68_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNX11834.1"
                     /translation="MNTDTFTTLPYKVADITLADYGRKEIELAEKEMPGLMALRQKYG
                     ETKPLKGARIMGSLHMTIQTAVLIETLAALGAEVRWCSCNIYSTQDHAAAAIAAAGFP
                     VFAWKGETLEEYWWCTLQALTFEGHKGPNVIVDDGGDATMMIHIGAAAEKNEAVLDKQ
                     TDGEDERELYKILKVVLKEDNGIWNRMAPDIKGVSEETTTGVHRLYQMLEEGSLLFPA
                     FNVNDSVTKSKFDNLYGCRESLADGIKRATDIMLAGKVVVVCGYGDVGKGCAKSMRAY
                     G"
BASE COUNT         5588 a         4379 c         4446 g         5799 t
ORIGIN      
        1 ctgaacgtgg aaaaagccat gcagcacaaa gtgctcgaca gcgaagaaat ccgcaatatt
       61 tatactgctc tcggtgtaac catcggaaca gaggacgaca gcaaggaact gaacctctca
      121 aaactgcgtt atcacaaagt gattatcatg accgatgccg acgtggacgg aagtcacatt
      181 gcaacgttgc tcatgacatt cttcttccgc cacatgaaag aaattgtgga acaggggtat
      241 ctctatattg ctactccacc gctataccgg tgctacaaag gaaaaataga ggaatattgc
      301 tggactgaac agcaacgcca ggcttttatt gaaaaatacg gtggcggcag cgaagcaggt
      361 atccacaccc agcgttacaa agggttgggt gaaatgaacg aaattcagct ttgggacacg
      421 accatgaatc ccgataaccg tactctgcgt caggtaacta tcgagaatgc agcaacagct
      481 gaccacgttt tctccatgct catgggcgat gatgtggcac cacgccgaca gtttattgaa
      541 caaaatgcaa catatgccaa cattgatgca taaaggatta taattaaatt tgtagggaga
      601 attgaatgcc tgattagttc gggtattcaa ttttctgtaa aaaagcaatt catttataat
      661 aggatagcaa aaaaagcgtt ttttaatttt aagtctgtaa taagccgatg cgtattgaag
      721 aaccagctta atccggtttt ttcgttcatc cgttttttta tcgtaaaaaa acattcattt
      781 aatccatttt caatacaata atgaaagtct gcgtattctg ttcgtcaagc aataatatcc
      841 ccgaaaagta caagcaatcg gcttaccggt ttggtcagtt tctggcgaat gaggggatta
      901 ctctggttta cggaggtgct acgggcggtt tgatggatgc cgtagcgcag ggagctaaag
      961 cgggcgatgg tgaaattatc ggcgttattc cggatatcat catcaaaatg aaccgtttga
     1021 gccacttgcc cactcagcaa atcatcgtat ctaccatgag cgaacgaaaa atgcgactga
     1081 agcaggaagc tgatgccttt gtggtgttgc ccggcggcta cggtacactc gacgaaatgt
     1141 ttgacgtcat tgcttcgggc aatgtaggtg aacaccgtaa accgctgata tgtgtcaacg
     1201 aagatggatt ttaccgacaa ttactgggac agatagccac catgagaacc gaatgtttca
     1261 ccccgaaaaa tgaaaattac aaaccagttt ttgtggcaac cctggaagaa tgtatgaaac
     1321 aaatacgtat tttgcatact aataaatagt gtactatgaa tctattctgg aaaaagagat
     1381 ttggcgggat gttatccacg tcgaaattcg agaaaatgga agaagcggca gtcagtgaca
     1441 tgaaacgcta cgacgagata gccaaatcac tggaactcga agagtaccga aaactgtttc
     1501 atgttgtgaa gtcgtcggaa ttcaaagaga aaaagaaaac actccagaac cgtaaatatc
     1561 aggatacgga ggaataccgc atcagccgca agtatcaaaa actgcacacc aacagcagta
     1621 tcagacagta ttatgaaaca ctccattcgg agagactaaa cacttttctg gatttcaaga
     1681 ctactcccga atacgaagaa ctgggcgaca aaaagaaagt aaaagcctcc gaaaaacttc
     1741 aaaaactgaa agactttgaa aattcgaagg aatataaaaa ttacatacgg tatcacgatt
     1801 cgtttattat ccgggattac gaagaactga aaaagaaaat tgccacccct gaatttgtaa
     1861 aatccaacca gttctgggct gatcagcatc gctggaaaac ggtacccgaa catgctgtgg
     1921 agcaacggta ctatgagctg gctaaaaatc cggatatcat tttttatgaa aatgaaaaac
     1981 cggcgcgatt cgaaaaatac agaaaattgt cacccgcatt catcgaagat ttcgattggt
     2041 acacgctcga taagtcgcgc tgggcatacg gctttcatgc ctccaacgaa cagctcatag
     2101 gcaactattc gttcgacaat gagcgtcagg caaatagcgg aggtaagaat gtaagtgttg
     2161 tacatggcac tttcaccctc gaaacaaagc acgaaaaatg catttcacgg gcatggaatc
     2221 ccaccaaggg attttatgaa aaagaatttg actacacctc cgatgtggtg cagactgccg
     2281 atgaatttcg tcagacctac ggcattttca aagcaaaaat acgctgtaca ggtcgtgcac
     2341 accatgcatt ctggctcagc aataactcga aacttccgca catcaatatc ttccattacg
     2401 acggtaaagc cataaccatg ggtaatgccc acgaaaatgt gatggatgag gtacgaatta
     2461 ccggactgaa acacagcagc ttttacatct ataccctgga gtggacaccc aaagaactga
     2521 tctggtacat caatgacctg gaagtgcatc gcacaacctc caatgtgccc aaagaaaatc
     2581 tttatcttgg atttaattca ttccttccgg caaaagaaaa accatctacc ggaaaacttg
     2641 aagtggattg ggtgaaagtg ctttctttca attaaaatca taagatttat gttgcagaaa
     2701 ttctgccgtt taccgcagaa atgtaacaat aacaaagact tgtaatggaa aaaccaaaaa
     2761 taatagccga aattggttgt aatcacaagg gtgaaatgga tatagcgctc gaaatgatta
     2821 aaactgcagc tacattttgt catgtggatg tggtgaagtt tcagaaacga aacaatcatg
     2881 aattgctcac cgaggaggaa ttcaatgcac ctcatccaaa tccctataat tcctacggag
     2941 ctacctacgg tgagcatcgc gaataccttg aattctcgct cgaacagcac cgccagctcc
     3001 aaagctggtg taatgagttc ggtgtggagt attcctgctc ggtgtgggat gttacttcgg
     3061 ccaaagaaat tatttctctc aatcccgcca ttatcaaaat tccttcggcc tgcaatctca
     3121 atcaggaatt gctgacctgt atttgcgacc attttgatgg tgaaatacac gtttcgtttg
     3181 gtatgaccga cagagaagag gaagaaaaaa tagtatcctt ttttgaaaag aagggacgta
     3241 acaaagagtt ggtaatttac agctgcactt ccggatatcc cgttccgttt gaagatattt
     3301 gcctgctcga actcacccga ttgcgaaaaa cttacgaaag ccgcgtgaaa gccattggct
     3361 tttcgggtca tcacctggga attgctgttg atgctgctgc cgtagcgctg ggagcggagt
     3421 gggtcgaacg acattttaca ctggaccgta cctggaaagg cactgatcac gcggcatcgc
     3481 tcgaacccga cggactgcgc aaactctgcc gcgattgccg ggcagtgtat aaagcactga
     3541 aatataagga agaagatatt ctggaaatag aaaagccaca actggagaaa ctaaaacgaa
     3601 accagatacg atggaacgac gacaagtaac tgcattcata ccggtaaggg gaggaagtaa
     3661 atccattcca ctgaaaaata tccgggaatt ttgcggcaaa ccactggttt actggaatat
     3721 tgcagcactg caggcagccg gattggttga taaaatcgtt gtagccaccg attctgacga
     3781 catcgaagct acagtccgtt cctttggatt tgataaggtg gaaatatacc gccgctcggc
     3841 agaaaatgca tcggatacag cgagtaccga aagtgtgatg ctcgaatata ttgaggctgc
     3901 caatttgtcc gaagaaactg ttttcatgct ggtgcaggct acatcaccgc tcacgcagac
     3961 cattcatttt caggaagcac tccaactgta ttccgaaggc aactacgatt cgttgctgac
     4021 ctgtgtggta aacaaacggt ttttttggaa taaaaacgga acaccccgaa attacgatta
     4081 catgaatcgt ccacgcagac aaaatttcga tggagaactg atggaaaacg gagcttttta
     4141 tatcaatacc gttaagaata ttctgcagtc aggtaatcgg ttgagtggaa agataggaat
     4201 atatgagatg cccgaataca ccgccatcga aatagatgaa cccgatgact ggacgatgct
     4261 cgaaaacctg atgcgaaaac atgtgctgaa aactcaaccg gtaacaaaga tcaaattgtt
     4321 tctgaccgat gtggacggcg tacttaccga cggcggcatg tattactctg aaattggcga
     4381 tgaacagaag aaatttaata cccgcgatgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn attatttccg ttgcaccaca atttttgagt gaatagtcac gggcttgttg
     4561 gcttattttc tgcaattttt catcgtcaaa ttcaagcggc gtaaaccagg catccagttc
     4621 ttcgatattg ctgactgatg cacaaattcc caattccacc atttccaatc caggccggtt
     4681 tttgttgtaa ttagggccaa agacggcagg cattccatag atggtagctt caatcacgct
     4741 gtgaataccc gctacaaatc caccgccgat atatgcccaa ttgccgtagc ggtacaatct
     4801 tgccagcata ccaattttat ctacaatcag cacctgtgta ttggtaaagt ctgtgtcagg
     4861 agtacattcg gagtaaataa gaacttcacc ggtggtattg gctacaattt tgttcatcgg
     4921 ttcgcggtcc atttcgtgag gtacgagcag aaaacgggtt tccggatgtt tgttgatcag
     4981 cttctgtagc aaatcttcgt caccggcacc aacggtactt ccggcaataa acagtttccc
     5041 atcctgcctg aatttctcca caatttcatc tttccattct tcctttgaaa tgctcagaac
     5101 acggtcgaaa cgcggatcac cggccaaaat tgcgttggtc aaaccgatag ttttcagcaa
     5161 ttctatggaa cgactatcct gaacaaacag gacttcaaaa gtgctcagtg cttttcggaa
     5221 aacgccaccg taccaacgga aaaaacggga gttgggaata aagcgggcgg aaaccacaaa
     5281 ggtgcgtact gatcggttct taagttcagc caggaggttt ggccaaaatt catatttaat
     5341 aaaaacggct atttcgggat gcaccacatc caggaatgca cgtacatcgc gggtagtatc
     5401 ggccggaaga taacataccc gatcggcaag agggtagttt ttatgtgcat tgtagcccga
     5461 aggggaaaag aaggtgacta aaatcctgta gttcgggtag cgggaacgga tttcctcaat
     5521 cagcggacgt ccttcttcga attcgccgaa cgaagctgca tgaaaccaaa ccacccggtc
     5581 ggaaggttta atatcttttg ccaggctttc ggccagatga cgtctgccgt caagcccttg
     5641 ttttattttt ttattccaca atgaagctat ttttgctccc ataattacgg tttttacacc
     5701 gagatccatc caccacatac gctattttcc gatttttgta caaagataag aatttcagat
     5761 ttaatttgca ggattttgac gatttgcctt actttgtgtg gaaaagtgaa aacctgctga
     5821 tcgtgagatt taagaaaatt tacattgaga taactaacag ttgtaattat cattgcagct
     5881 tttgttttga caccagccga ccgaaaaaat ttatgtcgcc ggaggaattc agtcttgttt
     5941 tgagtgaaat acggcctttt accgattaca tttatcttca tgtgcttggt gagccgcttt
     6001 tgcacccgaa cattggcaga atactacaat tgtcggcaga agcaggtttg aaagtgaata
     6061 ttaccaccaa tggaagtttg cttcagaaac agaaagaatt actcctgcaa catccggtaa
     6121 ggcagataaa tatatcgctc cacgatgccg aagaaaatat tcagcctgaa aaatggaagg
     6181 agtttgttca gactatgttg aactcagcta ctgatctttc gaagaatacc tacatcaatt
     6241 ttcgcctgtg gaatcagacc agtaagtcaa gcaaggaatt taatgatctc tgtttcagtc
     6301 tgataaaaga cacatttcag ttagaagaaa aacatgatct ttctgcccaa agcggtcgga
     6361 atatcactct ggctcccaat atctggctgc aacttgcccc acgatttgaa tggataaaaa
     6421 acgagaccac tcaggtgaaa aattgctacg gtctgcgtga tcagatagcc atactgtcgg
     6481 atgggagcgt ggtaccctgc tgcatcgatg ccgatgccca cctgctgctc ggcaatattt
     6541 ttacgggtaa acttaccgat atcttacaat cagaacgtgc tcaacgcatc aaaaatggtt
     6601 ttgaacaaca caaagccatc gagccgtact gtcagtcctg cggattcaga gctgaataaa
     6661 tcctcttcat tttttatccc acaatgatgg tatggaaaat accatttacc ggtgattgta
     6721 ctgaactgcc ggcagcagta tctttgcttc atacttaaaa gaaaaaaaaa gatatgaaac
     6781 gcctatgtcc aactaaaaat tttggacaca taatggtatg attaaataac gtacaacaaa
     6841 tgatagacgc tcaatattcc ccctttaggg ggttaggggg ccgctaaata aattataaac
     6901 acatgaaaac aactgctata tgccccattt cggataaaag aataaatgaa aatgttgcca
     6961 ggggaaacgc aacccttact gttttgtttc tgaccgcttt cgcaatcact tccaacgtat
     7021 tattaattgt gtttttgttg attgatttcc tactgcgcgg gatggaatta tccagatata
     7081 gtccttttgc cattatttgc agcaaagtgg tgaagctttt gggtatcaaa ccaaaaataa
     7141 taaatgccgg tcctaaactt tttgcagccc gcattggcgt atttttcagt ctggctattc
     7201 tggtgtcgtc actttcaggg cttgaaatca cagcttacat tctgacgggc gtttttggag
     7261 tgtgtgcatt ccttgaagca ttcttcgggt actgtgtagc ctgcaaaatt tatccgtttg
     7321 tttaccgtac attttatgct gcttcctgtc agcaatcctg atttttgcca gttgagattg
     7381 ctgtctatat tcattttcta aagtgatttt ttttattttc atttcctaac aaaaaaaggt
     7441 tcagaaagca ttgactttcc gaacctttta cgcatttaat atattgaatt atatgttgtt
     7501 agccttcatg tagtttttca gcgggttggc atacatttcc agcattttgt accgaaggaa
     7561 atcacggtct ttattctcca aatcaatttt acggatttgt ttgtccaaat attcttcaaa
     7621 tgttgttttt tcctctgtgc tgtaatcctt tgcaaagtcc ttatcaccgt gaatttcgtc
     7681 gtaggcaatt ttattttctg cggtgatgct gaagtttcta tggatatgat aatcaatcag
     7741 atgtgctgcc cgctctatct gttcgttcct gttgggagtt tcacgtgcta ttgcatccag
     7801 ttcgccgttt atgcagcccg aaatatgata aaccactttt cctttataac ccatcacgcc
     7861 ggtttgcata tttatcaggt cgtcctccgg tgatttttta tagtcgggat tatcgcgttt
     7921 cagttgaaat tccttggctt tcagaaaatc gcacgggtcg tattggtacg aaatgctgag
     7981 cggacagata ttcaactctt tcagattgtc gataaaagaa ccggaattgc cgcccaggtt
     8041 gaacattttc aacaggcttt cctgagtacg atcgtcggag tctttggcgc gtccttcgcg
     8101 ttgtgctatc catattgagt ttttcctttc tttcatggca tggcgcaggt atgccgaaag
     8161 gcgtttggag ctttccaaaa cctgacgtgc attcagtccg cgctgtacaa taaaactttt
     8221 atttactcgt accaggtctt caatccatgg gaaaacgagc aggttgtcac cgatggcaat
     8281 ttcaacggta tcgagtttat tctcaataag tttggcgcaa agcagcgccg aatccagtat
     8341 tatatcccgg tgattggaaa tgtacaggta tttttgatcc cggtcaatat tttccagacc
     8401 atggagtacg ataccggtgg tcatattggc ttccaggtac tgcagaaacg gataaaccat
     8461 ctgctgttgc aactcattgg aggtggtgaa gctcagcaac cgttgcttta actgttcctt
     8521 gggcataagc gggaagaggg tgcttacaac cttcataaat ggtttttggc tgcaaagccg
     8581 ttccatcgct gcgggtatat cgtcggtgtt gtacgaccgg atatcatcaa aattatattc
     8641 ttccattctt ttgcttagtt aatcggtttt tatttttttg aaaaaagttg cgggattaaa
     8701 aaacgaaact caaccagaga aaaattacag gcgctgacat aagcatgctg tcaaagcgat
     8761 ccagcagtcc accatgtccg gggatgatgt tgcccgtatc tttcacattt tcagtgcgtt
     8821 ttatgagaga ttcggtcagg tcgccgaaag tcccgaaaat aacgatgatt tcggagaaaa
     8881 tgaaccactg aagcaggttc agttccggaa tgaaaagtga aaaaacatat cctgaaatga
     8941 gtgcaaaaac ggctccgcca ataaacccct cccaggtctt tttgggtgaa atgcgttcaa
     9001 aaagtttgtg tttgcccagt agcattccgg atacgtaggc tccggtatcg ttgacccaga
     9061 tggtaataaa tacggcaagg agtatgatgg gttggtaatc gcttacataa aggataaaac
     9121 tgagaagcga gaagggtaaa gctataaata actgtccgag aataaaatag gcgaggttgt
     9181 tgaccggatt ttgctttttt cgatacaatt cggctataaa aactcccaca ataaacaatc
     9241 cgtaaaacga ataaatcgga tactgaaacc ttccggaagc ataaagaaat gaacatataa
     9301 acagcagaac agcaccgata acggctacgg ttttgttgac ctgaacttcg gccggtttgt
     9361 tggtaagttt gtgaaattca taggctgcca tagccgtaaa taatgcaaaa acagctgcaa
     9421 aagtataagg cgagagcaga atggaggata tggtaatagc aacaaatatg cccccgctga
     9481 tggatcgttt tacgaaatta ttcatacaac ttatgttttt gaagcaatac gtttaagcag
     9541 ggtttgtacc tacggcgaat ttataatgca aagttattaa aagatttatc attgcaaagt
     9601 aaaacgataa attctttcgg tccgtgagct cctaaaacca acgttttttc aatgtctgat
     9661 gtccggctgg gtcctgtgat tgtggttatt tgtgaaggaa gatgttctgc atattttttc
     9721 tgtaaagcat catacccatc agcaggatat gccaccagct gatttgtacc ggccaacacg
     9781 atatgaacgg gagggtagga gagcagttgt ctgcccgact caccggccga tgaaaccact
     9841 acactgcctg ttctggcgat gaggaattca cagggtgtaa cagctgctga catggtctca
     9901 aaatcgtcct gacagccggt aaaagcaatt ccgtgtttcg tgagatgtgt tccgatttgt
     9961 ctgtcacggc agaaaatagt atcaatattc ctgtcgttga ggattttttt aagttgaaca
    10021 aaaagttcat cctgcgtttc agatagttca cattgtccgt ttacagcttc aagttctgtt
    10081 ttgaaacagg tgacagcatt gggttcgatg ggtttgtaga tttcgtcatc gggggaaacg
    10141 gaaaaaacag acccgaattc acgggtctgt tttgcttctg atattctctt cagtatttta
    10201 tctctggaag aattattgtt cattcttttc ctggtttgtg tctgctgtgc cggttttttc
    10261 ttcacctcca tctgtttcgt cagatgaagg tagttcagct ttttgttccg cttcctttgc
    10321 tttttcccgg agggcattgg catcttcgtt gagacgcatc aactcatcgc tgcgtgactc
    10381 ccacggacgt ttgccaaaaa ttctttccac atcttcggca aatataactt cctgatcaag
    10441 aagaatttcg gccagcgtat tatgaccggc agcattttcc ttcagtattt gtttggcacg
    10501 ttcgtactgt tcggccacta tcaggcgggc ttcttcgtca atggtttcgg ctgttttttc
    10561 gctgtatggc tttgtaaatc cataatcgga atttccggta gagtcgtaat agctcatgtt
    10621 tggcaatttc tcactcattc cgtagtaagc caccatggca tacgcccgtt tggtaacgcg
    10681 ttccaggtcg ttgatagcac cggtagatgt ttgcgtgaga aacacttctt cagctgcccg
    10741 tccgccgaga gtggcacaca tctcatcgag catgtgctcg cggttggtta gctgacgttc
    10801 ttcgggaaga taccaggcag cacccagtgc ctcgccacgc ggaacgatag tcactttcac
    10861 cagtggatta gcataacgca gtttccaact gatggtagcg tgccctgctt cgtgataagc
    10921 gattgatttt ttttcttcta ctgtggtaat tttgtttttc ttttcgagcc caccgatgat
    10981 acgatccaca gcatccagaa aatcctgttt gcccacgaat gatttggctt tacgggcagc
    11041 tatcaatgcg gcctcgttgc atacattggc aatgtcggct cccgaaaatc cgggagtctg
    11101 gcgagccaga aaatccacat caacggtgtt gtcaatttta ataggacgca gatgcacatt
    11161 gaaaatttcc tttcgttcgt gtacatcggg caggtcaacg tgtatttgac ggtcgaacct
    11221 tccggcacga agcagcgctt tgtccaggat atcggcacgg ttggtggcag ccaggattat
    11281 tacaccgctg ttggaaccaa aaccatccat ttcagtcagt agctgattga gtgtgctttc
    11341 gcgttcatcg ttgctgccca tgttggggtt ttttccacgc gcccgaccta cagcatcaat
    11401 ttcatcgata aaaatgatac acggagcttt ttctttggcc tgacggaata aatcacgcac
    11461 acgtgaagca cccacaccca caaacatttc cacaaaatcg gaacccgaca tggaaaagaa
    11521 cggtacatcg gcttcgccgg ctactgcttt tgccagaagg gttttaccgg ttcccggagg
    11581 gcctacaagc aaggcacctt tgggtatttt tccacccagt tcggtatatt tcgtcggttg
    11641 tttgaggaat tcgactattt cttccacttc ctgtttggcg ccctgaagtc cggctacatc
    11701 cttaaaggtg attttattct gtccgtcacc cttgtcaaag agctgtgctt tggatttgcc
    11761 aacactgaaa atgcctccgc cggctccacc gcccattccg cccatacggc ggttcatcat
    11821 ccagaacagg aaaaccataa gtataatcgg tccgaaaccc cacagaataa ggttcagata
    11881 atccttattg gttttgtatt ccacctgaat ttcccggccc agggtcttac ttgcgttgtc
    11941 gattgttttt ttagcttctt cgggagaacc aatcaacagg cttattgccg gtgctttctt
    12001 gttgtaggtg gagtcttttc caaatacata actggcagaa tcttttatta taaatgcctg
    12061 aagcgtattg ttgctgtaaa tgtacacttt ttcaatataa cctttattca gatattgttc
    12121 gtattgagtt acattcactt gtttggacgg tgtgtcgttg ccaatgagat aagacgctaa
    12181 taatccgatc attataagcg catatatcca tgtaaaatta aactttgagg gtttgccacc
    12241 ggttttgagt ggtttgggag ttggattttt attttccatt aataaacagt gtgtttttta
    12301 ttttttttta attctgataa attcttcaat tgtaatatgt gtatgataat tcctgaaaat
    12361 atccatttgt caggaattta tttacaattt aacaatctgc aggctgtttt tgtttgaatc
    12421 tgcggatgat ttaatccaga tcgggaataa aattgaggtc ggcatcttcc cacaggtgtt
    12481 cgaggtcgta aaactcccga aacggacgct ggaaaacgtg tacaattatc tgaccgtaat
    12541 ccatggctat ccactggcag ttttcgaaac catcagttgc atagggctta atattcgttt
    12601 tttcgagtac ccaatctttg atagaagtgc ctatggcatt aacctgtgtg ttggaatcgc
    12661 cctgacagat cacaaaataa ctgcagggag catcgttgag tttgttgaga tttaccacta
    12721 caatgttttt tccctttttt tcctgaattc cttctactat tttttcaact ataacctcgt
    12781 tttcaatcat tcgtattttt ttcttttgat tttgtttttg tactaataaa gttcagtgtt
    12841 ccctttttgc atcagtcgtt tttcaaaaca tgtatgcagc tttcaaacct aaggtgtaca
    12901 aagcagcatt gttagttccg gtaaatttac cccacggtgt agtctcagtt accattccgt
    12961 ttatttgcct tacctgagcg aataatccaa aaaataagcc ggtgttcccc gttaactgtt
    13021 gattaactcc tccgctgatt tcggccatcg ggccgccttt atagattttg ttcagcgcaa
    13081 aatcgtaacc ggttttaatg ccgcccgcta tttgtttgct cctgtcgctc tgaatttgaa
    13141 taaataccgg caggtaattt aagttattat ttttttcaaa aattgtttcc acacccaggc
    13201 caaagcccag aaacgcattg ctatgaaagg cattgcgggt acccagcgcc agtgaaagat
    13261 gcgctgaagg tgatagcgat acagctcccg gagctgaggc ggcacctccg tttacactga
    13321 ttattcctgc gaaatgactt ccggctgttc tgttgccatt agcgtccacc tgaatttccc
    13381 gtaaagtttc ctgttttatt ttctcgattt caccaacccg aaactggtat ctttttccat
    13441 ccccggtttt cagcatcact atctcatcgg tacgcatcac aatttcaccc cggtaacgtt
    13501 cgccggtttt gagcgtgatt acactctgtg taaccgcttg ctgggcaaaa gtactgatgc
    13561 tcaaaagcaa aaacattgct acgagttgga taagacgctt catcgatcag gaatttaatg
    13621 cattgctgaa acgactgaga aataaatcgg catcctgttt cgatatattg agcggtggga
    13681 gcaaccgtat cacgtgtgtt ccgctggctc cggtgaatac tttttgttcg aacagcaagt
    13741 tgttgcgaag ttgcttcacc ggttcgttca tttctatgcc tatcatcagc cccatgcccc
    13801 taatttcttt tatctgggga aatgcattta atttcttaat cagataactg ccaacggtag
    13861 ctgcattttc cacgagtttt tcccctttca tcacgtccag aacggctata gctgcgctgc
    13921 atgccaggtg atttcctccg aaagtagttc cgagcattcc gtgtaccggc ttaaatagcg
    13981 ggctgatgag cactcccccg atggggaatc cattgcccat tcctttggcc gtagtgatca
    14041 tgtcaggtcg gatgcctgcg tgctgatgag cgaaaaattt tccgctacgc ccatagcccg
    14101 attggatttc atccagtata agtatggtgc cggtggcggt acaaacttcg cgcagtgatt
    14161 tcaggaattc cggttcgggt acccgtatgc cgccaacgcc ctgtatgcct tcaatgatga
    14221 cggcacaaac atctttgttt ttcagtgctt ccttaacagc ttgtatatca ttcaacggca
    14281 aaaattccac atcgagatct tcgttgaggg gagctacgat gttgggatta tcggtcaccc
    14341 gaacggcggc cgaggtacga ccatgaaagc ttttgctgaa agccaccact ttttttcggc
    14401 cggtgtgaaa ggaggcaagt ttcagcgcat tttcgttggc ttcagcaccc gaattgatca
    14461 gaaacagcga ataatcctca tatcccgaca gtttaccaag tttttcggcc agtttcacct
    14521 gaagtgaatt ctgcaccgaa ttggaataga aaaccagttt ttccatctgt tcttccattt
    14581 tctggatata ataaggatgt gaatgaccaa tggaaataac ggcatgaccg ccgtacatat
    14641 ccaggtattc tactccgttt ttgtcgtagg tataacatcc tctccctttt gttatttcga
    14701 tatcaaacag gggataaaca tcaaatggtt tcatctctaa ctttttctga ttattttatg
    14761 caaaagtagc gattttttcg ctgataataa acaataagac actaagctcc cgatattcct
    14821 gaattaattt caggctgtta tgcagcgaac ttagtgtctt gctgtctgtg ctgtattcta
    14881 ttcttccgtt gagcgtataa ggctacagta tttcaatact ccgttttaca aacttagcca
    14941 aagcctctcc cttgagcata ttgttggcca gcagagcaag gtctatcagt tgtccgactg
    15001 ctttatttcc tgaagcaaaa tttttataaa ccgttttttt gccttctgtc agttcatcta
    15061 ttttctgttg caggctgtcg agttcttctt tttcagcagt aggaatttca tcctcttttt
    15121 tgtccttgtg actgtctttt agcgatgtct gacggttttt cagttcgctg agttgtgccg
    15181 tgaaaggcag aacctctgct tcagtagttt cctgttcttc tttttgcagt ttctcaatga
    15241 gcggatgagc cgtatttatc accaggttgt agctgtcggg catttcaccg taaaaattca
    15301 tcatacctcc attctgcatg gctgccattt ctttcatgcg gcgcataaat tcgttttggg
    15361 taatgaaaac gggattggct tcgggcgaaa gtttctcgaa ggttacgatg aagtttgttt
    15421 tttcgatgga tggtaactgc gagtcgaaaa tggtaatgag tgcatcgcgc tgttcgtccg
    15481 ttaattctac ttttccggtg tcttccttgc gaatgagttt ttcaaccaca tcgctgtcca
    15541 cccgaacaaa gcgtgttttt tccagttttt gctccagttg gctgattgcg tgcacatcca
    15601 gttgtccgtc catgagcagc acatcgtatc ctttggccct ggcagcttcc aggtgtgtgt
    15661 attgttcgtc cttattggtg gcgtagaggt aaatcaggtc accgtcctta tctttctggt
    15721 tgccttccac caaatttttg tattcttcga atgtgaagta ttttccttcc acatttttga
    15781 ggaatgcaaa cttttcagca cgttcgtaga atttctcatc gctcagcatg ccatactgga
    15841 tgaatagctt caggtcatcc catttttgtt cgaattgtgc gcggtcggtt ttgaaaatat
    15901 cattcagtcg gtcagccact tttttggtga tgtgtgatga tattttcttc acattggcat
    15961 cgctctgcag gtagctgcgc gaaacattaa gcggaatatc cggtgagtcg atcacaccgt
    16021 gaagcagggt aaggtattcg ggcacaatgt tttctacgtg gtcggtcaca tacacctggt
    16081 tgcagaaaag ctgaatttta tttcgctgaa tatctatgtt atttttaatt ttcgggaaat
    16141 aaagaatacc ggtcaggttg aacggataat ccacattgag gtgaatatgg aaaagcggct
    16201 cgtcgctcat ggggaaaagt tcgtgataga acttatcgta atcttcgtcc ttcaaatcgg
    16261 ctggtttgcg tacccatgcc ggatttacat cgttgataat gttgtcttta tccgtttcta
    16321 cctgtttgcc gtctttccat tctttctgtt ttccgaatgc aatttcaaca ggcaggaatt
    16381 tacagtattt tgtcagcagt tcctgaattt tgctttcttc gaggtaatcc aggttgtcat
    16441 cgtcgatgtg cattacaata tctgtaccgc gatcggcctt gatggtctct tcgagtgtat
    16501 attcggggtc gccgttgcag gaccagtgct ggcctgtagc tccttcgcgg aacgattggg
    16561 tgaaaacttc cacttttttg gaaaccataa acgaggaata gaagccaagt ccgaaatgtc
    16621 cgataatgtt ctgagcatca tttttgtatt tttccagaaa ttcctcagca cccgaaaatg
    16681 caatctgatt gatgtatttg tcaatttctt cggcagtcat cccaatgccg cgatccgata
    16741 cggtcagttt tttcttcttt ggatcaacgc ttaccctgat ggtcagatct ccaagttcgc
    16801 ctttgaactc gcccacggct gccagtgttt taagtttctg ggtagcatcc acagcattcg
    16861 aaatgatttc acgcagaaaa atctcgtgat cggaatatag aaatttcttg attacgggga
    16921 aaatattatc acttgttacc ccaatgtttc cttttgccat aaacagtatg ttttttttat
    16981 tgttaatttt gtagttgaca gatttcggta aatattccct cgaaatctgt atgccattcg
    17041 ttttgcaaat acgatgccaa acagcatgaa ctgacagaat ggcagattgt cggtgaattt
    17101 tgactgaaaa caggacaaaa ttaaacatta ccgataaaat atcgttatta aatgaaactc
    17161 atagaacatc atgaacgaaa gaattaaaac gaaagcgaaa aactcaactg aaggtcaggt
    17221 attagtgatt tttggcgcaa acggcgattt gtcgcaccga aaattactcc ctgcaatatt
    17281 ccatttgttt gtggataatc tcctgcctga caaatttgca gtcataggtg cgggcagcga
    17341 cgagaagacc gaagaaacgt ttcgcaccga agtacgcact cagcttgcac aatttgcacc
    17401 ggaaactgtt gccgataatc ccgataaact gaccaaattt ctcgaattgc tttattacaa
    17461 gcctgtcaac aataaaataa aggaagattt tgacggattg aaatcttaca ttaacacgct
    17521 gtgtacccgg ttggatattc cccgaaacct gatttattat ttctccattc caccgtttat
    17581 gtacgaagtg gtggcggctc atttggtgag cagtggcctt aatactgaga aagatggctg
    17641 gaaacggatt attgtggaga agcctttcgg ttacagttat gattcggcag tggaactcga
    17701 cgggaaactg catgatggtt tcgccgaaca tcagatttat cgtattgacc attatctggg
    17761 taaggaaacg gtacaaaaca tcatggtcac ccgctttgcc aacggattct tcgaacccat
    17821 ctggaaccgc cggtacgttg accgtgtgga aatcacttca gcagaaaaaa taggggtggg
    17881 caatcgcggc ggttattacg acacctcggg tgccatgcgc gacatgattc agaatcacct
    17941 cctgcaggta ctggctatcg tagccatgga gcccccggct gtattcgaat cagaatcaat
    18001 acgcaacgaa agtgtgaagg tgcttcaggc actgcgtcct ataaatccga acgaagtgga
    18061 aaaatatgta atcaggggac aatacctgca atcggctaca gatggtgaat tgatgcaggg
    18121 ttatcgtcag gaaaagggag tggctccggg ttcaaaaacc gaaacatatg ttgggatgaa
    18181 agtctttgtg gataactggc gctggggcga tgtgcccttt tacattcgca ccggtaagcg
    18241 tttgccttcg cgcgtgaccg aagtggttat ccacctcaaa ccggcaccgc tccaactatt
    18301 caaacatcgc tgcatcagtc agtccaccaa tatgattata ctgcgcatcc atcccgatgc
    18361 cggaattgcg gttgattttg gcatgaaaat tcccggagcg ggttacaagg tacagaatgt
    18421 gaatatggac tttcactatt ccgatttggc acaaaataaa atacccgaag cctacgagcg
    18481 tctgctgttg gattgtatga ttggcgactc caccctctat gcccgtgccg atgcagtgcg
    18541 tgccagctgg aaatttgtag atccgatact cacagcctgg aaaaataacc ccgatattcc
    18601 gctttatttc tacgaaagca acacctgggg accaaaagaa gctaatcagc tctttgataa
    18661 taaactgctc aaatggcttc caccaagttg taaattcaac tgcgaatgat atgactgaaa
    18721 ataatatgta taccttcgaa acaaccgaag aagttgcccg ggctgtagcc cgggttattg
    18781 cagtaaatac aaaccggcaa gaacgaaaca agcggtatta caatgtggcg ctctcgggtg
    18841 gaaatactcc gaaactgttg tttcagatat tggcatccga atttcaggaa aaaataagat
    18901 ggcaagcgtt acggcttttt tgggtggatg agcgctgtgt gccgccatcg gaccatgaaa
    18961 gcaactacgg catgacgtat gagaatttgt tgaaaaatat atatattccg gtaacaaata
    19021 ttttcaggat gaaaggggag aatgatcctt ctgaggaagc tgtaaggtat caggcactgc
    19081 tcgaaaagga attaaagctg aaaaagggaa ttccgcagtt tgatttggtt ctgctgggca
    19141 tgggcgacga cggacatacc gcatcaattt ttcccgacaa cctcgcgtta cttcatgcag
    19201 atgaaactgt agccgttgcc aggcatcccg tgagtggaca aatgcggatt accctcaccg
    19261 ggaaaatcat cagacaggct cgtcagcgga tgtttctcat caccggaaaa tccaaatcga
    19321 atgtattaaa tgaaatactt aaaaacgaag ttccggctca aaaatatccg gcttttcata
    19381 ttctttccct cccagacaca gaggtttacc tcgaccggga agccgcatct tttttaggat
    19441 aaaaaaaaat agtacctttg tagccctaat attaaaaaat aaatgaatac agacacattc
    19501 accacattgc cgtacaaagt ggccgatatc acactggccg attacggacg aaaagaaata
    19561 gaattagctg aaaaagaaat gcccggtctg atggcgcttc gccaaaaata tggagagaca
    19621 aaaccactga aaggggcccg cattatgggt tcactacaca tgaccataca gacagctgtg
    19681 ctgattgaaa cactggcagc ccttggtgcc gaagtacgct ggtgcagttg taatatttat
    19741 tccacccagg atcatgccgc tgctgccatt gctgctgccg gatttccggt ttttgcctgg
    19801 aagggcgaaa cactcgaaga atactggtgg tgcaccctgc aggcactgac attcgaagga
    19861 cataaaggcc cgaacgtgat agtagatgat ggcggcgatg caactatgat gatacacatt
    19921 ggtgcagcag ccgaaaaaaa tgaagccgta ctcgacaaac aaaccgatgg tgaagatgaa
    19981 cgtgagctat ataaaatcct gaaagttgtt ttgaaagagg acaacggaat ctggaaccga
    20041 atggcgcctg acatcaaggg tgtatccgaa gaaaccacaa cgggtgtaca ccgcctgtat
    20101 cagatgctcg aagaaggatc gctgcttttc ccggctttca atgtgaatga ttcggtaacc
    20161 aaatcaaaat ttgacaacct ctacggttgc cgcgaatcgc tggcagatgg cattaaacgc
    20221 gctaccgaca ttatgcttgc aggtaaagtg gtggttgtgt gcggatatgg cgatgtgggt
    20281 aaaggttgtg ccaaatccat gcgggcttat gg
//
LOCUS       DAOPZK010000022        19569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2894_length_19569_cov_21.955417, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000022 DAOPZK010000000
VERSION     DAOPZK010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19569
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2894_length_19569_cov_21.955417"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      1..95
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            155..2569
                     /locus_tag="PKH68_02490"
     CDS             155..2569
                     /locus_tag="PKH68_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX11835.1"
                     /translation="MRKLFLINLLFLIPFCISAQNFIRGNVVDENDLPLSKVIVTLTQ
                     SKQTATTNDNGEFEFAGLKDFAYKLSAKYIGYETGEQIVKTGSTVIVRMKPLEIHLED
                     VVVTATRATAGTPVAFTNVSKKQIGERNAGQDLPYLLMLTPSFIATSDAGTGIGYTGF
                     RIRGTDANRTNVTVNGIPYNDAESHSVYLVDMPDFASSLNSVQVQRGVGTSTNGASAF
                     GASINMQTETLNPVTYAEIGTSVGSFNTMKNTLKAGTGLIKNFAVDVRLSNLVSDGFI
                     DRASANMKSYYASAGYYGNKTMLKFMTFGGKEKTYQAWNGVDLDNFPRTYNELGKYTD
                     SYGNTQYYKNQTDNYGQYHYQLHLTQILSPSLSLNAALHYTRGIGFYEEYKTDRKYAE
                     YGLTPAIVNGTVLKKTDLVRQKWLDNHFGGATFSLNYQKEKYSLIVGGAANQYAGDHY
                     GKVIWVRNATNLNVDHQYYFNKSTKTDANIYGKGNYHLFNNLLAYIDLQYRYIQLNMN
                     GKDDKYDAETGTMRVLSNQKPFHFFNPKVGLNYKINTANNVFASFAVANREPNRNNYT
                     DAGPTEKPTSERLYDTELGYNHQSDGFSFGANVYYMKYKNQLILNGKMSEIGELLTSN
                     IPDSYRTGIELASGVKITGGLRWDGNLNLSRNKILNFTEYVDMYDADWNWTDTKVNQF
                     EKTDISYSPDLIANSIISYSFRNFETALYSTYVGKQYLDNTSNNDRSIDPYFVNNLRI
                     GYSLKLKKLKSIDFNLLINNLLNTEYETNGYNWYSYYLDGKRVNEKRYFPQAGINFLG
                     SVTIKF"
     gene            complement(2613..3317)
                     /locus_tag="PKH68_02495"
     CDS             complement(2613..3317)
                     /locus_tag="PKH68_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNX11836.1"
                     /translation="MMPTLYLIPTTLGETALDTILPAQNDQIVISLKYFIVENIRTAR
                     RFLKKVNREINIDELTFFELNQHTSPEEISTFLKPMQEGQDMGVISEAGCPAVADPGA
                     DVVAIAQQRNYVVKPLVGPSSILLSLMASGFNGQSFTFHGYLPIQQSDRAKMLKKMEV
                     QIYNNNQTQLFIETPYRNMKMLEDILTVCMPDTKLCIAVDITLETEFIKTKTVKEWKS
                     QKPDLNKRPCIFLLYK"
     gene            complement(3322..5265)
                     /locus_tag="PKH68_02500"
     CDS             complement(3322..5265)
                     /locus_tag="PKH68_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX11837.1"
                     /translation="MISIEQLSVEFGGSPLFDNIGFLINQKDKIALVGRNGAGKTTLL
                     KLIAGKQQPTRGKIIASTDLTIGYLPQHMIHNDGTTVMQEAEKAFEHITQLQDDIVRL
                     TQELADRTDYETEAYGAIINKLSHTNEHLQIINSGNFYAEIEKTLLGLGFVRSDFDRQ
                     CSEFSGGWRMRVELAKILLQDPDVLLLDEPTNHLDIESIQWLENFLVNTGSAILLVSH
                     DKAFIDAVTNRTIEISLGRIYDYSVNYSKYLELRKERHEQQVRAYENQQKAIQETEDF
                     IERFRYKATKAVQVQSRIKQLEKIDRLEVDLEDTSRLSLKFPPSPRSGSIPLEIEHLS
                     KAYGDHLVLENIGLIINRGEKVAFVGKNGEGKSTLVKCILDEIAYSGILKLGHNVKIG
                     YFAQNQASLLDENLTVFQTIDYVAVGDIRTKIRDILGAFMFGGEASDKKVKVLSGGER
                     SRLAMIRLLLEPVNLLILDEPTNHLDMPSKDVLKEAIRAFDGTAVIVSHDREFLDGLV
                     TKVYEFGNKKVREHLGGIYDFLQNKKIENLNELGLSKSKTATIDNSPKEISENKLSYE
                     ARKELNKKLRKAEKAVEEAEAIVSALENEIANMQKQLEIPEKASDPEFIMQLQKKQRE
                     LEQKMYEWEIFAEELEELQSAQQ"
     gene            complement(5262..6392)
                     /locus_tag="PKH68_02505"
     CDS             complement(5262..6392)
                     /locus_tag="PKH68_02505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNX11838.1"
                     /translation="MNQILHFISTHPYLVLLFSFTIGYLFMPVVIEVARKYNFVVSPN
                     KRTSHNGHIPNIGGINIFVSFLFTVFLFSYNVFSDIQFAVLGLFIILIVGFIDDLIDI
                     KASWKLIGEVVAAFFLIVVADLRLPSFHGFMGIYELPLLISYAVSFFVFIVIVNSLNL
                     IDGVDGLASGLGIIYSLFFAIYFQLSGHVDLSITAYAMVGSLVVFFYYNVFSNKRKIF
                     MGDSGALLLGYMINLFVFSFCEMNASHSLPPRFFINAAPALAISLLIVPLFDTVRVMV
                     TRMKNGHSPFNADKNHVHHLLLGLGLKHRDVSFILMGITLGFLILGIILRNLSNGMLV
                     SIDFIICLLLTKYLWTLVDRNNIQNKKNKVTKKKPAEFNKKP"
     gene            complement(6389..7852)
                     /locus_tag="PKH68_02510"
     CDS             complement(6389..7852)
                     /locus_tag="PKH68_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNX11839.1"
                     /translation="MIYGTRSAGIAIAKMLRTNEKIKYKLVGFVDDDRRSIEKVVMGV
                     RVYLKTEENVKKIISKKAKAIIVSPLKMETINPQNDLDVFLNHQMSVLIAPPMSIWDG
                     DKMPTAKQIKSVQIEDLLERPQIKISTENISAEINNKVVLITGAAGSIGSEIVRQIAV
                     FKPRLIVLLDQAETPMHDLILRLKEHYPKQDFSSYLADVRNIDRMKSMMDLYRPDIVF
                     HAAAYKHVPLMEDNPTESIQANVLGTKNMADLSVRYGVSRFVMVSTDKAVNPTNVMGA
                     SKRIAEIYVQSLNRKQEIDGKVVTKFITTRFGNVLGSNGSVIPYFKHQIEKGGPVTVT
                     HPDIIRYFMTIPEACLLVLEAGTMGKGGEIYIFDMGEPVKILDLAKKMIRLAGFIPEV
                     DIPITFSGLRPGEKLYEELLNQKEITRKTHHPKIMIATVREYEFEQISQEIDKLIKYS
                     YLGKNFLTVSQMKTIVPEFISNNSQYERLDVENIIAE"
     gene            complement(8482..10119)
                     /locus_tag="PKH68_02515"
     CDS             complement(8482..10119)
                     /locus_tag="PKH68_02515"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNX11840.1"
                     /translation="MIRKTILFCVLFSVFTASFAGEVRPTKNVILMISDGTSLSVLSA
                     SRWLKTYRNEGNKLNIDPWLCGTVTTFSSNAPIGDSAPTTSCYMTGITGQASNISVYP
                     VADPANDLIRLNPDSAYQPLATLLEAMQIEQKKSAGLVVSCEFPHATPADCASHYYNR
                     NDYKVLAPQMAYNNLEVMFGGGTDFVTDDMKKHFQNTGTTYIQNDKNAVLNFTGNRIW
                     GLFGPREMPYDLDRDTATVPSLAQMTSKAIEVLNKNENGFFMMVEGSKVDWAAHANDP
                     AAILSEFLAFDQAVGAAIDFARKDGNTTVVILSDHGNSGLSIGKSNMSKSYTRMTLQD
                     LMGSVSNYRKTAEGLEKILINTKPENIKEVFKQYTGIELTDDEMKSLKGSKNYRAESY
                     MDATHVENMNFFITRIMNSHSNFGFTTGGHTGEEVFLAVYHPQGDRPTGNIRNKEVND
                     YLVRAAGLNTSLKQLTTDIFAKHADVFAGMKYNIVQEKDKPAKLIVKQKRKTLEITAF
                     SSVATLSGKPLDLGSVVVFMDKNNTFYLPKNLRMILE"
     gene            complement(10131..>12530)
                     /locus_tag="PKH68_02520"
     CDS             complement(10131..>12530)
                     /locus_tag="PKH68_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNX11841.1"
                     /translation="HKEALKFLYAYMSLNDLGDYNSDFFYRQTKAAFEARDYFSWGKK
                     VPEELFRHFVLPPRVNNEDLDTARIVFFNELKNRIKDMNMTDAALEVNHWCHEKVTYR
                     PADSRTSSPLATVRTGYGRCGEESTFTVTALRAVGIPARQCYTPRWAHTDDNHAWVEV
                     WADGKWYFMGACEPEAELNKAWFTYPAKRAMMVHTNVFGKYDGPESKTEYPLYTKINV
                     LENYTITKKLDVIVVDENSNAVSGAKVKFLIYNYAEYYPIYEQVADQKGKASIITGLG
                     DVMVWAAKNGKYGYRKVAVGVSDEVLVQLDKTTGKDYEEEWDIKPPVNLATFTTDTTA
                     KTAENNKRLHFEDSLRNNYLSTFMKGDSARTLAKKLGLNAEKVVDFITKSEGNYSEIS
                     EFLIQNADNPNALNLLSTLTDKDLRDTPAAVLQSHLENSKNSGNLPDDVFIQGVLSPR
                     ISNELIRNWRPFLQKEFKGIFPSQPTVDDIKQWMNKHIQVVEDENYSGCLISPIGVYK
                     LKKTDKNSRKVFFVALCRSLNIPTVVDPATGEIRQYLQGKWNTVTLDTKAEKPATGKL
                     TINYSSQNGLIPQYWTYYTVSKFTDGFFQSLDFEGDKRVEKFPVQLDLEEGYYCITSG
                     NRYADGRVLARNEYFTILPNKTITKNLIIRALQPEKKIYGALKSSSLSSDLYQRKGMV
                     ICFLEPDREPTKHILNELPNHKSELDKWGGNFVFVVPEDKLSKNFEANNYKNLPQNST
                     FITRQGIEIMNDFLKSTNQSFRDNYPLIYIVNPQGKIIFYSEGYRIGVGDLIWKTVTT
                     N"
     assembly_gap    12531..12630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12631..12938)
                     /locus_tag="PKH68_02525"
     CDS             complement(<12631..12938)
                     /locus_tag="PKH68_02525"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HNX11842.1"
                     /translation="MKLNKLTPEEESVIIHKGTERPFTGEFVHNDKSGTYICKRCDAP
                     LYRSSDKFESSCGWPSFDDEIPGAVKRVSDADGRRTEIICAACGAHLGHVFEGEGFTP
                     "
     gene            complement(13143..13616)
                     /locus_tag="PKH68_02530"
     CDS             complement(13143..13616)
                     /locus_tag="PKH68_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNX11843.1"
                     /translation="MIQRIQTVYLILVTVLMTLAAVLPVAEYFDVAKNIVYQLDMRGF
                     VQLNPDGTFLSAISTNPVTFIFGIILVVTIMTIFKYKNRKQQFRLCTVNFLLILIYTI
                     VLAVVIFVGKNKLVGTELTLKIPAVFSIVALILNYLAMRGIAKDENLVKSMDRLR"
     gene            complement(13633..14658)
                     /locus_tag="PKH68_02535"
     CDS             complement(13633..14658)
                     /locus_tag="PKH68_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX11844.1"
                     /translation="MVKFAVIGQGHIGKRHAEMIRRNEDAELVAVCDILPKEQLGLAD
                     IQEPFFTDIDSLLEAGLDIDVVSICTPNGYHAEYAVKALKASKHVVLEKPIALTKNDA
                     ERIIFKSLKMSRHVFCVMQNRYSPPSVWLREVMDQRLLGKIFMVKLDCYWNRDDRYYK
                     KGNWHGDAKLDGGTLFTQFSHFIDIMYWLFGDITNIRGNFADFNHKELTDFEDSGVVT
                     FDFINGGMGCLNYSTSVWDTNLESSITIIGEKGTIKVAGQYMNEVAYCHVKDYTMPEL
                     APSNPPNDYGLYKGSAQNHHYVIENVVEKLTDKGSITTNVLEGLKVVEIIERIYQVRD
                     LQWKKEK"
     gene            complement(14683..15258)
                     /locus_tag="PKH68_02540"
     CDS             complement(14683..15258)
                     /locus_tag="PKH68_02540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNX11845.1"
                     /translation="MSYFAHPTAVIDENCIIGDGTKIWHFSHIMSGCVIGEQCNIGQN
                     VVISPKVELGRNVKIQNNVSVYTGVICEDDVFLGPSMVFTNVVNPRSHVNRKNEYAST
                     LVRKGASIGANATVVCGNEIGEYALVGAGAVITKAVKPFALVVGNPARQTGWVSRNGH
                     KLTFNDEGVAVCPETGEKYKLKEGLVKLISE"
     gene            complement(15293..16426)
                     /locus_tag="PKH68_02545"
     CDS             complement(15293..16426)
                     /locus_tag="PKH68_02545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNX11846.1"
                     /translation="MKPIQMVDLKSQYEKIKLQINAGIQEVIDSAVFIKGGKVVEFQH
                     QLEEYLHVKHVIPVGNGTDALQISLMALGFRPGDEIITPTFTFIATAEVVALLGLTPV
                     VVDVDPDTFNISLESIRAAITSRTKAIVPVHLFGQNADMEAILDLAREFDLKVIEDAC
                     QSIGSVYTFSDGRVVHSGCMGDIGCTSFFPSKNLGCYGDGGAIFTNNDELASKIQAIA
                     NHGMVVRYHHDLVGVNSRLDSIQAAILDVKLKYLDEYIASRQRAAAFYDNAFAECDKI
                     TIPYRNTNSTHVFHQYTIKLNGVDRSALQNYLSELGIPAMVYYPVPLHLQKAYKNSRY
                     REGDFPIAEMLSSCVLSLPMHTELDDEQLEYITKGVLEFIEKK"
     gene            complement(16475..17350)
                     /locus_tag="PKH68_02550"
     CDS             complement(16475..17350)
                     /locus_tag="PKH68_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HNX11847.1"
                     /translation="MFWFVHSLGRERESIINIPVNYIGIPEDVKIISELPRNITIVIR
                     DEGLRLLEYNKKKTIPLTLDLQRVYFQKGEILITADQLKTRLYSYVLPTTAVLSIKPD
                     SIAIHYYKLATKDLKIKLNATISLANQYILSDSIHVEPSNVKVFGPRHIVDTMKAAYT
                     EPVELKDIADTTLIKTKLRPITDVKYAFDDVNVGVFVEMFTEKKQQIPVTLINVPETM
                     NIRVFPVMIEATYNIGLSNFNKIKSNDIQAVFDYNEVTKNQKRRHKLQVINHSPYISS
                     LRFSPDEVEFLLEKK"
     gene            complement(17476..17814)
                     /gene="yajC"
                     /locus_tag="PKH68_02555"
     CDS             complement(17476..17814)
                     /gene="yajC"
                     /locus_tag="PKH68_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNX11848.1"
                     /translation="MNLLSILLQAATGAAQPSGGMGGYSGILMMVLIFVVFYFFMIRP
                     QSKRQKEIKAQREAMKVGDKVVTSGGIYGKVKDIKETTITVEIAENVRITVDKNSVFA
                     TAADLPNEVK"
     gene            complement(17847..18839)
                     /gene="nusB"
                     /locus_tag="PKH68_02560"
     CDS             complement(17847..18839)
                     /gene="nusB"
                     /locus_tag="PKH68_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNX11849.1"
                     /translation="MKKFLPLHQINDASATIFFMINRALLRIKIIQILYSYYKGDGQS
                     ISLAEKELFFSIEKTYELYFHLLQFSVEITHYAALKIDSRRNKLRPTEEDLHPNTRFI
                     DNRFVAQLSRNNTLQEFLKENKTSWVNNQDAIKSIYELIVASESYADYMNAETSDYTA
                     DKDIWRKIYKKIILMNEEFSDSIEDQSIYWTDDLEIVISFIIKTIKRFDTENGVNQEL
                     LPMFKDDEDAEFASRLIKNALENETLYRQMIDQHTNNWELDRIAFMDIIIMQAALAEI
                     MTFPTIPVNVTLNEYIEISKSFSTDKSATFINGVLDNIVKELKADNKLIKVVKI"
     gene            19091..19510
                     /locus_tag="PKH68_02565"
     CDS             19091..19510
                     /locus_tag="PKH68_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNX11850.1"
                     /translation="MEVEKRKIEIEKKDSEIIYSKAIKAGKRIYYLDVKKSRNEDLYL
                     AITESKKKVTGFDEDAQVTYEKHKIFLYKEDFDKFIEGLEDVVGFIKKEQGEAQPRAE
                     YTPDYKKEETKEETITGSIAEETSTEAKRSFFGKFKF"
BASE COUNT         5656 a         4001 c         3813 g         5999 t
ORIGIN      
        1 ttcaacaatt ggggtgcctt aataatttcg ggctgagatc ataccctttg aacctgcacg
       61 ggtaattccg cgaagggaaa aagatataat cctttttaca ctaccgtatt tttaggctct
      121 ctcacgcgaa tattaatcat ttaattaatt caaaatgaga aagttatttt taatcaatct
      181 tttattttta attcctttct gtatttcagc acagaatttt atccgcggca acgtggtgga
      241 tgaaaacgat cttccgttat cgaaagtaat cgtcacactt acacaatcca aacaaactgc
      301 aactacgaat gacaatgggg aatttgagtt tgccgggttg aaagactttg cttacaagct
      361 aagcgctaaa tacatcggtt acgaaaccgg tgagcaaatt gtaaaaaccg gttcaacggt
      421 aatcgttcgg atgaaaccgc tggaaattca tcttgaagat gtggtggtaa ccgctacgag
      481 agccacagct ggaactccgg tagcattcac aaatgtttca aaaaaacaaa tcggcgagcg
      541 aaatgcggga caggatttac cttatctgct tatgcttact ccttcattta tagccacttc
      601 tgatgccgga accggaattg ggtacaccgg ttttcgtatc cgtggaacgg atgcaaaccg
      661 cactaacgta acggtgaatg gaatacctta caacgatgcc gagtcgcaca gtgtatacct
      721 ggtggatatg cctgattttg cctcttcact gaattctgtt caggttcagc gtggcgtggg
      781 aacttctacc aatggtgctt cggcattcgg agcaagcatt aatatgcaga ccgaaacgct
      841 aaatcccgtt acgtatgccg aaatcggtac atctgtcggt tcattcaaca ccatgaaaaa
      901 tacgctgaaa gcaggcaccg gactaataaa gaactttgcc gttgacgtac gtttatcaaa
      961 ccttgtctcc gatggattta tcgaccgcgc ttctgccaat atgaaatcat actacgcttc
     1021 agccggatat tatggcaata aaaccatgct gaaattcatg acattcggag gtaaagagaa
     1081 aacctatcag gcatggaacg gcgttgatct ggacaatttt ccgcgcactt acaacgaatt
     1141 gggaaaatat acggatagtt acggaaatac acaatattat aaaaatcaga cagataacta
     1201 cggacaatat cactatcaac ttcatctgac acagatttta agcccgtcgc tgagtttgaa
     1261 tgcagccctg cattatacgc ggggaattgg tttctacgaa gaatataaaa ccgaccggaa
     1321 atacgctgag tatggtctga cacctgccat agtaaatggc acagtactca aaaagaccga
     1381 tttggttcgt cagaaatggc tcgataatca ttttggaggt gccacttttt cactcaatta
     1441 tcaaaaagaa aaatattcgc ttatcgtagg cggggcagcc aatcagtacg ccggtgacca
     1501 ttacggaaaa gtgatttggg taaggaatgc aactaatctg aatgtagatc accagtatta
     1561 tttcaataaa agcacaaaaa cagatgctaa tatttacgga aaaggcaatt atcatctttt
     1621 caacaaccta ctggcttaca tcgatttaca ataccggtac attcaattaa acatgaatgg
     1681 gaaagatgac aaatacgatg ctgaaacggg taccatgcgg gtattatcca atcaaaaacc
     1741 atttcatttc tttaatccga aagtcggact gaactataaa ataaatacag ccaacaacgt
     1801 gtttgcttcc tttgcagtgg ctaaccgcga accaaaccga aacaattaca ccgatgccgg
     1861 acccactgaa aagccaacca gcgaacggtt gtatgacacc gaacttggtt acaatcatca
     1921 gtcggacggt tttagtttcg gggcaaacgt ttattacatg aaatataaaa accaactaat
     1981 cctgaatggc aaaatgagtg aaattggcga gctgctaacc tccaacatac ctgacagtta
     2041 cagaaccggc attgaattag cctcgggagt aaaaatcacc ggtggattgc gctgggacgg
     2101 aaatctgaac ctaagccgaa ataaaatact gaactttaca gaatatgttg atatgtatga
     2161 tgccgactgg aactggacgg acacgaaagt caatcaattt gagaaaaccg atatatccta
     2221 ttcacctgat ttgattgcaa acagcattat ttcctattct ttccggaatt ttgaaacagc
     2281 tctttattcc acatatgtgg gaaaacaata cctggataat acttcgaata acgaccgatc
     2341 aatcgatccg tattttgtaa ataatctccg gattggctat tcattgaagt taaaaaaatt
     2401 aaaatcaata gattttaatc tattgataaa taacttatta aatacagaat atgaaaccaa
     2461 tggttacaac tggtacagct attatctgga tggaaaaagg gtgaacgaaa aaagatattt
     2521 tccacaggca ggaattaatt ttctgggttc ggtaacgata aaattctgat ttttaccgaa
     2581 aatccttttt cttctccgaa aagggatttt ttttatttat aaagtagaaa aatacaggga
     2641 cgtttattca aatcaggttt ctgtgatttc cattctttta ccgttttagt tttgataaat
     2701 tctgtttcga gcgtgatgtc aacggctatg caaagtttgg tgtcaggcat acagacagtg
     2761 aggatatctt ccagcatttt catattgcga tacggagttt cgataaaaag ctgtgtttga
     2821 ttgttgttgt aaatctgcac ttccattttt ttcagcattt ttgctctatc cgactgctga
     2881 ataggcagat aaccgtgaaa agtgaaactc tgaccgttga accctgaagc catgagcgac
     2941 agtaaaatgg acgaaggacc gaccagaggt ttcaccacat aatttcgctg ctgagcaata
     3001 gccaccacat cggcaccggg atctgccact gcaggacatc cggcttctga gattactccc
     3061 atatcctgtc cttcctgcat tggtttaaga aaagtactaa tttcttcggg agaagtatgc
     3121 tgattaagct caaaaaaagt cagctcgtcg atattgatct cccggttcac ttttttcaga
     3181 aatcggcggg cagtgcgtat attctctaca ataaaatatt tcagcgaaat tacaatttgg
     3241 tcgttctgag ccggcagaat agtatccaaa gccgtttcac caagtgtggt gggaattaag
     3301 tagagtgtgg gcataatgtt gttattgttg tgctgattgt aattcttcca gttcttccgc
     3361 aaaaatttcc cattcataca ttttttgttc caattcccgc tgttttttct gcaattgcat
     3421 aataaattcg ggatcagaag ctttttcggg tatctccagc tgtttttgca tgttggcaat
     3481 ttcgttttcc aatgcggata caattgcttc ggcttcttca acagcttttt cggcttttct
     3541 cagcttttta ttcagttctt tgcgggcttc gtagcttagt ttattttccg atatttcttt
     3601 tggagaatta tcaatggtcg cggtttttga tttcgataat cccagttcat tcagattttc
     3661 tattttcttg ttttgaagga aatcataaat tccacccagg tgttcgcgta cttttttatt
     3721 accgaattca tagaccttgg ttaccaaccc atccagaaat tcacggtcgt gggatacgat
     3781 aaccgccgtt ccgtcgaatg ccctgattgc ttctttgagc acatctttgg aaggcatatc
     3841 caggtggttg gtaggttcgt cgagtatgag cagattgacc ggttccaata ataaacgaat
     3901 cattgccagg cggcttcttt cgcctcccga aagcactttc acttttttgt cggaggcttc
     3961 gccgccaaac ataaatgctc cgagtatatc ccttattttg gtacgaatat cacctacagc
     4021 cacataatct atagtttgaa atacagtcag attttcatca agcagggaag cctgattttg
     4081 tgcaaaatat ccgattttta cattatgtcc caattttaag atgccggaat aagctatttc
     4141 atccaatata cactttacca gcgtactttt tccttcgccg tttttgccta caaaggccac
     4201 tttttcaccg cggtttatta tcagtcctat attttcaagg actaaatgat cgccgtaggc
     4261 ttttgagaga tgttctattt ccagcggaat ggatcccgag cgtggtgatg gtggaaattt
     4321 gagactgagc cgcgaagtat cttccaaatc cacttcgagc cggtcaattt tttccagctg
     4381 tttgatgcgt gattgaacct gtactgcttt ggtagctttg tagcgaaagc gctcgataaa
     4441 atcttcggtt tcctgtatgg ctttttgctg attttcatac gcccggactt gctgttcgtg
     4501 gcgctccttg cgcagttcca ggtattttga atagttgacg ctgtaatcgt aaatcctgcc
     4561 gagcgaaatc tcgatggttc ggttggttac ggcatcgata aaagctttgt catgcgaaac
     4621 gagcaaaatg gcacttccgg tattaaccag gaaattttca agccattgaa tggactctat
     4681 atccaggtgg ttggtgggct catccagcaa aagtacatcc ggatcctgaa gcagaatttt
     4741 tgccagttct acacgcatac gccagccgcc gctgaattcg gaacattgcc tgtcaaaatc
     4801 acttctgaca aaacccagcc cgagcagggt cttttctatc tcagcataaa aatttccact
     4861 attaataatt tgcagatgct cattggtatg gctcagtttg ttgataattg caccatatgc
     4921 ttcggtttcg taatctgtac ggtcggccag ttcctgtgtc aaccgtacaa tatcatcctg
     4981 caattgagta atatgttcaa atgctttttc ggcttcctgc atcacagtcg tcccgtcgtt
     5041 gtgaatcatg tgttgaggca ggtatccgat ggttaaatcg gtagaagcaa ttatttttcc
     5101 acgggtgggt tgttgttttc cggcaattaa tttgagaagt gtggtttttc cggcgccatt
     5161 gcggccaacc aatgcaattt tgtctttttg attaataaga aaaccaatat tatcaaagag
     5221 cggtgatccg ccaaattcta cactcaactg ttctattgat atcatggctt tttattgaac
     5281 tctgcaggtt tttttttagt caccttattt tttttatttt gaatattgtt cctgtcaacc
     5341 agtgtccaga gatattttgt caatagaagg caaatgataa aatcaattga caccaacatt
     5401 ccattggata aatttcgtaa aatgatacct aaaattaaga aacccagtgt gatgcccatt
     5461 aaaataaaag acacatcgcg atgcttcaat ccaaggccca acaacaggtg atgaacatga
     5521 tttttatcgg cattgaacgg cgaatgtcca tttttcatgc gggtaaccat cactctcaca
     5581 gtatcaaaca gtggaacaat gagcaatgaa atggccagtg ccggagcagc atttataaaa
     5641 aatcgggggg gtaaactatg gctggcattc atttcgcaga aggagaacac aaagagattg
     5701 atcatgtatc cgagcaataa ggcacccgag tcacccatga agatttttcg tttgttggaa
     5761 aacacattgt agtaaaagaa tacaaccaat gaaccaacca tggcataggc tgtgatcgac
     5821 agatccacat ggcctgataa ctgaaaataa attgcaaaaa acagggagta aatgattccc
     5881 agccccgatg caagtccgtc gactccatct atgagattaa gtgaattgac aatgacaata
     5941 aatacaaaaa atgaaactgc atagctgatt aacaatggta attcatatat gcccataaat
     6001 ccgtgaaaac tgggaagccg caagtcggcc actacaatga gaaaaaatgc tgcaaccact
     6061 tcgccgatta gcttccaact ggctttaatg tcaatcagat cgtcaataaa acccactatg
     6121 aggataataa acaatcccag tacggcaaat tgaatatcag aaaaaacatt gtatgaaaat
     6181 aaaaataccg taaacagaaa agaaacaaat atattgatgc ctccaatgtt gggtatgtgt
     6241 ccgttgtgtg aggttctttt attgggactt actacaaaat tgtacttacg ggcaacctca
     6301 atgacaacgg gcataaataa atatccgatg gtaaaagaga aaagtaatac taaatacgga
     6361 tgagtgctta taaaatgtaa tatctggttc attctgctat aatattttca acatcaagtc
     6421 gttcgtactg agaattgtta ctgataaact caggtacaat tgttttcatc tgcgatacgg
     6481 tcaggaaatt ttttcccagg taactgtatt ttatcagttt atcaatttcc tgagatattt
     6541 gttcaaattc atactcacgc acagttgcaa tcataatttt tgggtgatgt gttttacggg
     6601 taatttcttt ctgatttagt agctcttcat acaatttttc acccggtcgt aaaccgctaa
     6661 aagtaatggg aatatcaacc tccggtataa aaccggctaa ccggatcatt tttttggcca
     6721 gatcgagtat tttcaccggt tcgcccatat caaaaatgta aatttcgcca cctttgccca
     6781 tggttccggc ttcgagtaca agcaggcagg cttcgggaat agtcatgaaa taacggataa
     6841 tatcgggatg agtcaccgtc accggtccgc ccttttctat ctgatgttta aagtagggaa
     6901 taaccgaacc attggagcca agcacattac caaaccgggt agttatgaat ttggtaacca
     6961 cttttccatc tatttcctgc tttctgttca atgactgaac atatatttcc gcaattcgct
     7021 tggaggcccc cattacattg gtgggattta cagctttgtc tgtagaaacc atcacaaacc
     7081 gcgaaactcc atatctgacc gataagtcgg ccatattttt ggttcccagc acattggcct
     7141 gaatactctc tgtgggattg tcctccatga gaggtacatg cttataagct gctgcatgaa
     7201 acacgatatc gggacggtaa agatccatca tgcttttcat gcggtctata tttcgtacat
     7261 cggccaggta tgagctaaaa tcctgtttgg ggtaatgctc tttcaacctc agtattaaat
     7321 catgcatggg tgtttctgcc tgatcaagca ggacaatcaa ccgcggtttg aaaacagcta
     7381 tctgacgaac aatttcgctg ccgatagaac ccgctgcacc ggtgataagc acaactttgt
     7441 tgttgatttc agcagatata ttttctgtag aaattttgat ctgaggacgc tccagtaaat
     7501 cttcaatctg taccgatttt atttgcttgg ccgtaggcat tttgtcacca tcccatatac
     7561 tcatcggcgg ggctatcaac actgacattt gatgattgag aaatacgtca aggtcattct
     7621 ggggattaat tgtttccatc ttcaaaggag aaacaatgat tgcttttgct ttttttgata
     7681 ttattttttt tacattttcc tccgttttca aataaaccct tacacccatc accaccttct
     7741 ctatcgaccg tctgtcatca tcaacaaatc ccaccagttt gtatttaatt ttttcattgg
     7801 ttcgtagcat ttttgctata gcaatacctg ccgaacgggt accgtaaatc attacaggca
     7861 aggtgttacc tgcattttcg gatatgaaat caaagatggt ttttgccgac aaacgtatga
     7921 aaaataataa aaagaaagat attactccat aaaaaagcaa agagtaaaag taataaatat
     7981 ttaagtccaa aaataattta accaggaagg ttattatcat cagtaaaatg acatttgaaa
     8041 atactgctaa aagaagtttg gcggcatcca catagcccga atatctgaga atgccggaat
     8101 aggtatgaaa aaaccaaaaa aagattatct gaaaaaacaa taaaataaaa agtcggccgg
     8161 gcagtgctaa gtttaatatt tcatcgtttg aaacagaaag atgtgagaca ataaaataag
     8221 agaaaacaaa cgagaatgta caaagcaaaa catcaacaag taatactaac cagcgtggta
     8281 gatattttat ggagataaaa tgcgaaaaaa tatctgattt tttgataaat gtccaggggt
     8341 ttttcatatt attcaagttt catatattat tccataaaca aacgtgcaaa gataatgctt
     8401 cttcacaaat taatgctcaa ttttaactta aaacagaaaa tcatcccctt gttttaagac
     8461 taaaaaatga tataaaataa actattccaa tatcattctc aggtttttag ggagataaaa
     8521 tgtattattt ttatccataa aaaccactac cgagcctaaa tcgagtggtt taccactgag
     8581 tgtagctaca gagctgaaag ccgtgatttc aagggtcttg cgtttttgtt tcactattag
     8641 ttttgccggt ttgtcttttt cctgtacaat attatacttc atacctgcaa atacatcggc
     8701 atgtttggca aaaatatcgg tggtaagctg cttcaacgaa gtatttaagc cggcagcccg
     8761 taccagataa tcgttaacct ccttgttacg gatatttccg gttggccggt caccctgtgg
     8821 atgatatacg gccagaaaaa cttcttcgcc ggtatgtccg cccgtagtaa agccaaaatt
     8881 gctgtgcgaa ttcattattc tggttatgaa aaaattcata ttctccacat gagttgcatc
     8941 catataactt tccgcacggt aattctttga tccctttaag gatttcatct cgtcatcagt
     9001 gagttcaatc ccggtatatt gtttgaaaac ttctttgata ttttcaggct ttgtattaat
     9061 gagaattttc tcaagtcctt cagcggtttt ccgatagttg gaaacactgc ccatcagatc
     9121 ctggagcgtc atgcgggtat agctttttga catattgctt tttcctatag aaagtccgct
     9181 gttaccatga tcggaaagta ttaccacggt tgtatttccg tcttttcggg caaaatcaat
     9241 ggcagcccca actgcctggt caaatgccag aaattcactt aggatggccg ccggatcatt
     9301 ggcatgagcc gcccagtcaa ccttactgcc ttccaccatc atgaaaaacc cattttcatt
     9361 tttattgagc acttcgatgg cttttgaagt catttgagcc aaggatggca ctgttgccgt
     9421 atctctgtcc agatcgtaag gcatttcccg gggtccaaac aatccccaaa tccggttgcc
     9481 ggtgaaattc aataccgcat ttttatcatt ttgaatatag gttgttcctg tattttggaa
     9541 gtgtttcttc atatcatcag taacaaaatc ggttccccca ccaaacatca cctccaggtt
     9601 attgtaggcc atttggggtg ccagcacttt gtaatcgttc cggttataat aatgtgatgc
     9661 acaatcggcc ggtgtggcgt gcggaaattc gcagctaacc accagtccgg ctgatttttt
     9721 ctgttcaatc tgcatggctt caagcagcgt tgccagcggc tgatacgcac tatccggatt
     9781 taaccggata aggtcattcg caggatcggc tacaggatac acggagatat tcgaagcctg
     9841 acccgtaatc ccggtcatat agcaggaggt agtaggtgcc gaatcgccaa tcggagcatt
     9901 ggacgagaaa gtggtgactg tgccacacag ccatgggtcg atattcagct tattgccttc
     9961 gttccgatag gttttgagcc agcgtgaagc agatagtacg ctgagtgaag tgccatccga
    10021 aatcatgagg attacatttt tggtaggtct gacttcccct gcaaaacttg ctgtaaaaac
    10081 cgagaaaaga acacagaata aaatagtttt tcgtatcatt ttttttaaaa ttaattcgtt
    10141 gttactgttt tccaaatcag atcccctact ccaatcctgt acccttcgga ataaaaaata
    10201 attttaccct gaggattgac aatataaatg agcggataat tatcacggaa agactgattg
    10261 gtacttttca ggaaatcatt cattatttcg attccctgtc gggttataaa agtgctgttt
    10321 tgaggtaaat ttttatagtt atttgcctcg aagttcttac tcagtttatc ttcgggcact
    10381 acaaaaacga aattaccacc ccatttatcc agttccgatt tatgatttgg cagctcattc
    10441 aggatgtgct tcgtaggttc gcgatcgggt tccaggaagc agataaccat tccttttctc
    10501 tgatacaaat cagaagataa actactggat ttcaaggctc cataaatctt tttctctggt
    10561 tgaagtgctc tgataatcag atttttggta attgttttat ttggcagtat agtaaaatat
    10621 tcattacgtg ccagcacacg accatcggca tagcgattgc cggaagtgat acaataatat
    10681 ccttcttcca aatccaattg aaccggaaat ttctcaaccc gtttgtcgcc ctcaaaatca
    10741 agtgactgaa agaaaccgtc cgtaaatttt gatactgtgt aatacgtcca gtattgagga
    10801 atgagaccat tctgagaaga ataattgatc gtaagtttac cggttgctgg tttttctgct
    10861 ttagtgtcaa gcgtcacagt attccatttt ccctgaaggt actgacggat ttcgcctgta
    10921 gcaggatcaa caacagtagg aatattaagg cttcggcata aagcaacaaa aaatactttt
    10981 cgcgaatttt tatctgtttt tttcagtttg tacactccga tgggtgaaat taggcatcct
    11041 gagtaattct catcttcaac cacctgaata tgcttattca tccactgctt tatatcgtca
    11101 accgtaggct gtgagggaaa tattccttta aattcttttt gcaggaacgg acgccaattg
    11161 cgaatcaatt cgttggaaat tcggggcgaa agtaccccct gaataaaaac atcgtcaggt
    11221 aggtttccgc tgttttttga attttccaga tgactttgaa gtacggcagc cggcgtatcg
    11281 cgcaggtctt tgtcggtaag tgtcgataac aggtttaatg cattgggatt atctgcattt
    11341 tgaattaaga attcagaaat ttcggaataa ttaccttcac ttttcgtgat gaaatctacc
    11401 actttttcag catttaatcc caactttttg gctaatgttc gcgcactgtc gcctttcata
    11461 aaagttgaaa gataattgtt acgcagagaa tcttcaaagt gaagtcgttt attattttcg
    11521 gcggttttag cggtagtatc agtggtgaaa gtagccagat tgaccggtgg tttgatatcc
    11581 cattcctctt cgtagtcttt acctgtggtt ttatcaagtt gtacaagcac ttcatcgctc
    11641 actcctactg caactttccg atagccatat ttaccatttt tggcagccca caccatcaca
    11701 tcgcccaaac cggtaatgat tgatgctttg cctttctgat cggcaacctg ctcgtagatt
    11761 ggataatatt cggcataatt gtagattaaa aacttcacct tagcgccact caccgcatta
    11821 ctattttcat caacaactat tacatccagt ttttttgtta tggtataatt ttcaagcaca
    11881 ttaattttag tgtacagtgg atactccgtt ttactttcag gtccgtcgta tttcccaaaa
    11941 acattggtat gcaccatcat agcacgtttg gcaggatagg taaaccaggc tttgttgagt
    12001 tcagcctcag gctcacaggc gcccataaaa taccattttc cgtcagccca aacttcgacc
    12061 caggcatgat tgtcgtcggt atgtgcccag cggggtgtat aacattgacg ggctggtatt
    12121 cccaccgcac gcaatgcggt gactgtaaag gtggattctt cgccacaacg tccgtatccg
    12181 gttcttacgg tagccaaagg cgatgaagtg cggctgtcgg ccgggcggta tgtcactttt
    12241 tcgtgacacc agtggtttac ttccaatgcc gcatccgtca tattcatgtc ttttatcctg
    12301 tttttcagtt cgttgaagaa aactatgcgg gctgtatcaa gatcttcatt atttactctc
    12361 ggtggtaata cgaaatgacg aaacagctcc tcgggcactt tttttcccca gctgaaataa
    12421 tcgcgcgctt cgaaggcagc ttttgtttgt cggtaaaaaa aatcgctgtt gtagtcaccc
    12481 aaatcgttga gcgacatata tgcatacaga aatttgagtg cttctttatg nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttggtgtaa atccttcacc ttcgaacaca
    12661 tgcccgaggt gtgcaccaca tgctgcacat attatttcag tgcgccggcc atcagcatcg
    12721 gacacacgtt ttacagctcc gggtatttca tcgtcaaagc ttggccagcc acaacttgac
    12781 tcgaatttat cagacgaacg gtacaaaggc gcatcgcacc gtttacaaat atatgttccg
    12841 cttttatcat tatggacaaa ttcaccggta aatggtcttt cggtgccttt gtggatgatt
    12901 acactttctt cttcaggggt taatttattc aatttcatag ctttagtgtt tttagtttgg
    12961 ccgcaggccg aaaaaacaac aaggagcagc agtaataaaa tatgctgtcc cctgtttgtg
    13021 taattactat attttataac tttcatatca cctgatttta ctatgttgaa aaacaattga
    13081 aatcaaattt tgtttcaagc cgatccgata ataactgaaa gttatttttt tgatttaaat
    13141 tcctacctca atctgtccat ggacttcacc aggttttcgt cttttgcaat tcccctcata
    13201 gccagataat ttaatatcag tgcaacgatt gaaaatactg caggaatttt caacgtaagt
    13261 tccgtaccaa ccagtttatt ttttcctaca aaaatcacca ctgccagcac gatggtgtaa
    13321 atcagaataa ggagaaaatt gacagtgcac aaacgaaact gctgctttcg gtttttatac
    13381 ttgaaaatag tcataatagt cactaccaga attattccaa atataaatgt aacaggatta
    13441 gtgctgatag ccgataaaaa tgttccgtcg gggttcaact gaacaaatcc gcgcatatcg
    13501 agctgataaa caatgttttt ggcaacatca aaatattcgg ctacgggcaa tacggcagct
    13561 aaagtcatga gaacagttac caatatcaga taaacggttt gaattctttg tatcatattc
    13621 ttttctgttt ttttactttt cctttttcca ttgcaggtca cgtacctggt aaattcgctc
    13681 gataatttcc accactttca gtccttccag tacattggtc gtgatggagc ctttatcagt
    13741 cagtttttcc accacatttt caatcacata atggtggttt tgagctgaac ctttgtataa
    13801 tccgtagtca tttggcggat tggatggtgc aagttcgggc attgtataat ctttcacatg
    13861 gcaatacgcc acttcattca tgtattgtcc ggcaactttt atggtacctt tttcgccaat
    13921 aatggtgatg ctgctctcca gattggtatc ccacaccgag gtggaataat tcaaacaacc
    13981 catgccaccg ttgataaagt caaatgtcac tacgccacta tcctcaaaat ctgtgagttc
    14041 cttgtgatta aaatcagcga aattgcctcg tatattggta atgtcgccaa agagccagta
    14101 catgatatca ataaagtgcg aaaactgagt gaacagtgtg cctccatcga gttttgcatc
    14161 tccgtgccag ttaccctttt tgtaatagcg atcgtcgcga ttccagtagc agtcaagttt
    14221 taccataaag attttgccca gcagacgctg atccatcact tcgcgaagcc ataccgaagg
    14281 aggcgaatat cggttttgca tcacgcagaa tacgtgtcgc gacatcttca gcgatttgaa
    14341 tataatccgt tcggcatcgt ttttggtgag tgctatgggt ttttcgagaa ccacatgttt
    14401 actggctttc agtgctttga ctgcatattc ggcatgatat ccgttgggcg tacatatact
    14461 aactacatca atatcaagac ctgcttccaa caaactatca atgtcggtaa aaaatggttc
    14521 ctgaatatcg gcaagtccta attgctcttt tggcaaaata tcacaaacgg ctaccagctc
    14581 ggcatcctcg ttgcgtcgga tcatttcggc atggcgtttt ccaatatgtc cctgccctat
    14641 tactgcaaat tttaccatat aattttttta tttcaatatt tattactcgg atatcagttt
    14701 taccagtcct tccttcaatt tatatttttc tcctgtttcg ggacaaacgg ccacaccttc
    14761 atcattaaaa gtgagtttat gcccgttccg gcttacccat cctgtttgcc gggcgggatt
    14821 gcccacgacg agtgcaaagg gttttacagc ctttgtaatc actgcgccgg ctcccaccag
    14881 tgcatattcg cctatttcgt tgccacacac caccgtagca ttggctccga tactggcacc
    14941 tttgcgcaca agcgttgaag catattcatt tttcctgttc acatggctgc gcggattaac
    15001 aacattggta aaaaccatgg aaggtcccaa aaaaacatca tcttcacaga ttacgccggt
    15061 gtaaaccgat acattgttct gtatctttac gttgcgtccg agttctacct ttggtgatat
    15121 cactacattc tgaccaatgt tgcattgttc gccaataacg catcccgaca tgatgtgcga
    15181 aaaatgccag atttttgtgc catcgccaat gatgcaattt tcatcaatca ctgctgtagg
    15241 gtgtgcaaaa taactcattc tgatttatta tatgactgat tattaaccta ttctattttt
    15301 tttcaataaa ttccaataca ccttttgtaa tatactccag ctgttcatcg tccagttcgg
    15361 tatgcattgg cagtgacaaa acacatgagc ttagcatttc agcaataggg aaatcacctt
    15421 ctctatatcg tgaattttta tacgcttttt gcagatgcag cggaacggga taatagacca
    15481 tcgccggaat gccaagttcg gataaataat tttgcaatgc agaacggtca acgccgttta
    15541 atttaatagt gtattgatga aatacatgcg ttgaattagt atttcgataa ggaattgtta
    15601 ttttatcaca ttctgcaaat gcattatcgt aaaaagcagc tgcccgctga cgtgaagcga
    15661 tatattcgtc aaggtattta agtttcacat ccagaatagc tgcctgaata ctgtcaagac
    15721 gtgaattaac tcccaccaga tcgtgatgat atctgaccac catgccgtga ttggctatgg
    15781 cctgaatttt ggaagccaac tcatcgttgt tggtgaatat tgcacctcca tcaccataac
    15841 atcccagatt tttggaagga aaaaacgatg tacaacctat gtcgcccatg catcccgagt
    15901 gaactacacg tccatctgaa aaagtataga ctgagcctat cgactggcag gcatcttcta
    15961 taactttcag atcaaattcg cgagccaagt ccagaatagc ttccatatcg gcattctgac
    16021 caaaaaggtg aaccggaaca atggctttgg tccgggaagt gatggctgca cgaatgcttt
    16081 cgaggctgat attgaatgta tcgggatcaa catccaccac tacaggagtt aaaccgagta
    16141 aagctaccac ttcggcggta gctatgaatg tgaatgtggg agtaataatt tcgtcgccgg
    16201 gtcgaaaacc gagtgccatg agcgaaattt gcaaggcatc tgtaccattt ccaaccggaa
    16261 taacatgctt cacatgcaga tattcttcca attgatgttg aaattccacc acctttcccc
    16321 ccttaataaa aacagcagaa tctatcactt cctgaatacc ggcgttaatt tggagtttta
    16381 ttttttcata ctgacttttc aggtcaacca tttggatggg tttcatcatt ttatgcaata
    16441 taaattttta aaatttcttt tttatgacaa caggctactt tttttcaagg aggaactcca
    16501 cctcatcggg tgaaaaccga agactggaaa tataaggtga atgattgatt acctgtagtt
    16561 tatgtcgtct tttctgattt ttagtcactt cattgtaatc gaaaactgcc tgaatatcat
    16621 tcgatttaat tttgttgaaa ttgctcaatc caatattata agtagcctct atcattacgg
    16681 ggaataccct gatattcatg gtttcgggca cattgatcag agtaaccggt atttgttgtt
    16741 ttttttcggt aaacatttca acaaaaaccc ccacattaac atcatcaaat gcgtatttca
    16801 cgtcggtaat gggtcttaat ttcgttttta tgagcgttgt atcagctatg tctttgagtt
    16861 caacgggctc agtataagct gctttcatag tgtcaactat atgtctgggt ccgaatacct
    16921 taacattgga cggttctacg tgaatgctgt cgctgagaat gtactgattt gccagcgaaa
    16981 tggtggcatt tagttttatc ttcaggtctt tggtagccag cttgtaataa tggatggcta
    17041 tggaatcggg tttgatactc aacactgccg tggtgggcag cacgtagctg tataaacggg
    17101 ttttcagctg gtcggccgtg atgagaattt cacctttttg gaagtaaacc cgttgtaaat
    17161 cgagtgtaag gggaatggtt ttctttttat tgtattcgag cagacgaagt ccttcgtcgc
    17221 ggatgacaat agtgatattt cgtggcaatt ccgagattat ttttacatcc tcaggaatac
    17281 cgatataatt gaccggaatg ttgataattg attcgcgctc gcgacccagc gagtgtacaa
    17341 accaaaacat gcctgaaagg acaagaaata ccaagaaggt caaaacattc ttagaaataa
    17401 gtaatgcttt aaccttcaaa attgagtttt ttatcgcctt aaatcctgac atgccggacg
    17461 tgtagcaaat aaaaattatt ttacttcgtt cggaagatcg gcagctgtgg caaacactga
    17521 atttttgtcc acggtgatgc gtacattttc agctatttct accgtgatag tggtttcttt
    17581 gatgtcttta accttaccgt atataccacc cgaagtaact actttatcgc ctactttcat
    17641 ggcttcgcgt tgtgccttaa tttctttctg gcgtttggat tgtggacgta tcatgaagaa
    17701 gtagaaaacg acaaagatca ggaccatcat caaaatacct gaatatccgc ccataccacc
    17761 tgaaggttga gctgcgcctg ttgcggcttg taacaaaata cttaataaat tcataagtgt
    17821 gtgtttatta gttgattaat tgataattat attttaacga cttttatcag tttattgtca
    17881 gccttaagtt ccttcacaat attatcgagc actccgttga taaaggtggc actcttatcg
    17941 gtgctgaatg atttggaaat ttcgatgtat tcatttaacg ttacattcac cggaattgtt
    18001 gggaaagtca tgatttcggc caatgccgcc tgcatgatga tgatatccat gaatgcgatg
    18061 cgatcgagct cccagttatt ggtgtgctgg tcaatcattt gccggtacaa agtttcgttt
    18121 tcaagagcat ttttgatcag tctggatgcg aattctgcat cctcatcatc cttgaacatc
    18181 ggcagaagtt cctgatttac accattttcc gtatcaaatc gtttgatggt tttgattata
    18241 aaactgatga caatttcgag gtcatcggtc cagtaaatac tctgatcttc aatcgaatca
    18301 gagaattcct cattcatcag aatgattttc ttatatattt ttcgccagat atccttgtcg
    18361 gctgtgtaat ctgaagtttc ggcattcata taatcggcat aactttcgga ggctacaatc
    18421 agttcataga tactttttat agcgtcctga ttattcaccc acgaagtttt attttctttg
    18481 agaaattcct gcaaagtatt gttacgtgag agctgtgcaa caaatctatt gtcaataaat
    18541 cgagtgttgg gatgcaaatc ttcttcggta gggcgcagtt tgttacggcg tgaatctatt
    18601 ttgagggctg cataatgggt gatttcgacc gaaaactgca agaggtgaaa atataattcg
    18661 tacgttttct cgatactgaa aaacaactct ttttctgcca aagatatgga ttgtccatcg
    18721 cctttgtaat aagaatataa aatctggata atctttatcc gaagtaaagc ccggtttatc
    18781 ataaaaaata tagttgcgct tgcgtcatta atttgatgca aaggtaaaaa ttttttcatt
    18841 tattacgccc atatcgtttc aaaatcttgt catttgggaa tttattaacg cttccactgc
    18901 tgtcatttga aaaataaata aaataaaagt ttctattcgt ttgataaatc aaaaaaatat
    18961 tcagaaattt gacccttgaa agagagtatt cgtacgcttt tcaagagata ttaccgaaat
    19021 cattactaat ttgcagcaaa aacccggcac tatttgagtt gatagctgtt ttaaaaatgt
    19081 accgtaattt atggaagtcg aaaagagaaa aattgagatt gaaaagaaag acagtgaaat
    19141 catttattcc aaagccatta aagcaggaaa aaggatttat tacctggatg taaagaaaag
    19201 ccgtaatgaa gatttgtatc tggccattac agagagtaag aagaaagtaa ccggatttga
    19261 tgaagatgct caggttacgt atgaaaaaca caaaatattc ctttacaaag aagatttcga
    19321 taaatttatc gaaggcctgg aagatgtggt aggttttatt aaaaaagaac agggtgaagc
    19381 acaaccaaga gcagaataca ctcctgacta caaaaaagaa gaaacaaagg aagaaaccat
    19441 aaccggttcc attgctgaag aaacaagcac cgaagcaaag cgaagttttt tcggcaaatt
    19501 caaattctga tttgtagtta caattccttt actgagcgtc cgcggaaaat ccgaacgggc
    19561 gctttttta
//
LOCUS       DAOPZK010000023        19374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2951_length_19374_cov_22.847145, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000023 DAOPZK010000000
VERSION     DAOPZK010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19374
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2951_length_19374_cov_22.847145"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1273)
                     /locus_tag="PKH68_02570"
     CDS             complement(239..1273)
                     /locus_tag="PKH68_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNX11851.1"
                     /translation="MTLLIVIIVLALIFDFINGFHDAANSIATIVSTKVLTPFQAVLW
                     AAFFNFLAFFIAKYIIGGFGIADTVSKTVIAEYVTLPIIFSGIVAAIIWNLATWWLGI
                     PSSSSHTLIGGFAGAAIAANMGNFAVVKGAVILKIASFIFLAPLIGMTVAALLTLFVY
                     WTFRRANPHKANEWFKHLQLVSSGLFSIGHGLNDSQKVMGIIAAALIATHIHHPEIGH
                     GIESVAQLPNWVAFACFTAISLGTMSGGWKIVKTMGTRITKVTPLEGVVAETAGALTL
                     YLTEFLKIPVSTTHTITGAIIGVGSVKRLSAVRWGVTQQLLVAWILTIPVSALIAAII
                     YFAFGHLLIS"
     gene            complement(1337..1984)
                     /locus_tag="PKH68_02575"
     CDS             complement(1337..1984)
                     /locus_tag="PKH68_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNX11852.1"
                     /translation="MLNSFFSKFTPREPKFFPLLKAMVDVIKDATDIMIEFIEKGNHE
                     NAAEYYRKIKEEERKGDALSNKIFDELNKTFITPFDREDINNLATHLDDVTDYINSAA
                     KRIFLYNPKVLPPSANELVQLIKEGSLMLEKAVDELDVLKKNAKNIKEYCYELHLIEN
                     KADDVYEHFLIDLFENAKDGVELIKQKEIMYELEKATDAAEHVGKIIKTIIVKYA"
     gene            2379..2546
                     /locus_tag="PKH68_02580"
     CDS             2379..2546
                     /locus_tag="PKH68_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX11853.1"
                     /translation="MAYVITEDCIACGTCISECPVEAISEGDIYVIDPDVCTDCGTCA
                     DVCPSEAIHPA"
     gene            complement(2608..3678)
                     /gene="wecB"
                     /locus_tag="PKH68_02585"
     CDS             complement(2608..3678)
                     /gene="wecB"
                     /locus_tag="PKH68_02585"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNX11854.1"
                     /translation="MKRIITIVGARPQFVKAATLSRLFGLMGVDEKIIHTGQHFDANM
                     SEVFFEEMEIPKPAYNLDIHGVGHGAMTGRMLEEIEEILLKEKPEGVLVYGDTNSTIA
                     GALAAAKLHIPLVHVEAGLRSFNMAMPEEINRILTDRISNLLLCPTDTAVNNLKREGF
                     ENYSCKIVKNGDVMQDAALYYVQKAEQKSVILSKLNSPEFVLATFHRQENTDSPQNLK
                     GIIDGLNAINKQITVIVPLHPRTRNILNAQKIVPEFTVIEPVGYFDMILLLKNCKMVV
                     TDSGGVQKEAFFFGKQCITLRNETEWVELVDNSYNVLTGSDSRKISEAFNWSVNKKSD
                     FSLNLYGNGQAAKIAASEIIAY"
     gene            complement(3675..4787)
                     /gene="wecB"
                     /locus_tag="PKH68_02590"
     CDS             complement(3675..4787)
                     /gene="wecB"
                     /locus_tag="PKH68_02590"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNX11855.1"
                     /translation="MKKLILVVGARPNFMKIAPVMHQLKDDKEIQPVLVHTGQHYDTQ
                     MSGRFFEELGIPAPDINLEVGSASHAVQTAKIMIGFEQVCLDEKPDFVMVVGDVNSTL
                     ACTLVAAKLNIKTIHYEAGLRSNDRTMPEEINRLVTDAVSDYFFTTSADADENLMREG
                     VDPKKIFMVGNLMIDTLEANLRKAQNSPQSFPLLNKTDKIDINDFSENGYGVMTFHRP
                     SNVDEKESLELLVDIWCRISKSIPLIFPIHPRTLGNIRKFGLMDKLNTSNVFLIEPLG
                     YLEFIHLVSNARFALTDSGGIQEETTHLNIPCLTVRPNTERPVTIWEGSNKLIKTEEI
                     VPEVSEILRGNGKTGQNPKFWDGHTAERIIEIIKNL"
     gene            complement(4803..6098)
                     /locus_tag="PKH68_02595"
     CDS             complement(4803..6098)
                     /locus_tag="PKH68_02595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX11856.1"
                     /translation="MKKVLIITYYWPPLGGAGVQRWLKFSKYLPKYDWEPIIYTPENP
                     DFAIEDQSLQKDISPGLVVIKRPIWEPYEIYKTLIGKRGQKVNVSFSEGGKKKSLIHK
                     FSMSVRGNFLIPDPRCFWINPSVRYLKKYLKENPVDAIISTGPPHSMHMIGLKLHKQV
                     HIPWLADFRDPWTNIDFYRELNLSWVADKIHHHHEKKVLSNADKVVSVTPTWCRELAE
                     IAGRTVDLVHNGYDEEDVIPTNKSVDAEFSISHIGSINSDRNPEILWVALSLLLEENE
                     EFRRSLRIKLVGNVEPVVFADLEKYQLTGFVEKVGYLSHQEAVEFQQTSQILLLLINN
                     TPNANGILTGKLYEYIASRRPVLCIGPPESDIANLLKETKSGSIVDFEDVDAMKKVIL
                     ELFEKYKNNRLQSTAENYEQYSRKAQCGIMAQLLDNITN"
     gene            complement(6136..7671)
                     /gene="gltX"
                     /locus_tag="PKH68_02600"
     CDS             complement(6136..7671)
                     /gene="gltX"
                     /locus_tag="PKH68_02600"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNX11857.1"
                     /translation="MERNVRVRFAPSPTGPLHIGGVRTALYNYLFARQHGGDMLLRIE
                     DTDSARFVPGAEEYVVEALRWLGVEIDEGIGADKEGAHAPYRQSERKEIYKKYVEQLL
                     ADGKAYMAFDTPQELDVQRSKIANFQYDASTRDMMQNSLTLTKEEVERRLSEGDQYVV
                     RIKIEPNIEIHVNDLIRGEVIINSSVLDDKVLYKSIDELPTYHLANVVDDYLMEISHV
                     IRGEEWLPSAPLHVLLYRYLGWEAVMPQFAHLPLLLKPDGNGKLSKRDGDRLGFPVFP
                     LQWEDPNTETVSSGYRESGYFPEAVVNFLALLGWNPGTEQELFSMKELIEQFSLDRVS
                     KSGAKFDYEKGKWFNHKYLQMKSNEELADIFQHILVEKDIIEDSEKVSRVVGLIKERA
                     NFVTDLWDQSFYFFRGPAEYDEKSVKKRWKQDSPERLTELTEVIQTMDDFSAANQEAV
                     ICKWIEDNGYGMGAILNAFRVAIVGAASGPHLFDITEIIGKEETVVRLQRAISFLSKQ
                     DEE"
     gene            complement(7842..8846)
                     /gene="tsf"
                     /locus_tag="PKH68_02605"
     CDS             complement(7842..8846)
                     /gene="tsf"
                     /locus_tag="PKH68_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNX11858.1"
                     /translation="MAVTMAEITKLRTMTGAGMMDCKNALIEAENDFEKAVEIIRKKG
                     QAVAAKRSDREASEGCVLADTKDGFGAVLALKCETDFVAKNADFIALTQSILDKALAE
                     KPESLEALKAITITDDGRTIGDLVTERSGITGEKLELDYYEFVKGGTVTAYIHPGNKL
                     ATIVAFAENDAEYETIHGVAMHIAAMSPVAIDESAVPQKVKDNELAVAIEKTKTELVA
                     KEVEVALKKAGINPAHVDSEDHIESNMAKGWITAEDAAKAREIKETVTAEKSASLPEQ
                     KVNSIAAGRLAKFFKEYTLVEQKYEGGGEEAGKTTVKELLAKKHLTCTGFKRVTLNQE
                     "
     gene            complement(9025..9858)
                     /gene="rpsB"
                     /locus_tag="PKH68_02610"
     CDS             complement(9025..9858)
                     /gene="rpsB"
                     /locus_tag="PKH68_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNX11859.1"
                     /translation="MSRTNFEELLEAGCHFGHLKRKWNPAMAPYIFMERNDIHIIDLH
                     KTVVKIDEAAAAMKQIAKSGRKILFVATKKQAKDVVADKATSVGMPYITERWAGGMLT
                     NFPTIRKAVKKMTTIDKMSTDGTFDNISKREKLQITRQREKLEKNLGSIADLTRLPSA
                     LFVVDVMKENIAVKEAQRLGIPVFGIVDTNSNPNDIDFVIPANDDATKSVEVILNAMC
                     AAIQEGLEERKVEKIDAEASEAPEMKEKKSRVSKRPRTQKDDDEALKANVVKKFVKEE
                     E"
     gene            complement(10084..10470)
                     /gene="rpsI"
                     /locus_tag="PKH68_02615"
     CDS             complement(10084..10470)
                     /gene="rpsI"
                     /locus_tag="PKH68_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNX11860.1"
                     /translation="MEVVNALGRRKAAIARVFVSEGKGQITINKRDLAVYFPSPILQY
                     VVKQPLNKIGVVEKYDIKVNLDGGGYKGQAEALRLAIARALVKINPESKPALKSEGFM
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            complement(10476..10931)
                     /gene="rplM"
                     /locus_tag="PKH68_02620"
     CDS             complement(10476..10931)
                     /gene="rplM"
                     /locus_tag="PKH68_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNX11861.1"
                     /translation="MDTLSYKTISANKATVQKEWVVVDATDLVLGRMGSKVAKLLRGK
                     YKPSFTPHVDCGDNVIVINAEKVKLTGNKWTDRIYLRHTGFPGGQREYTPSDLMAKDP
                     DRLIRKVVRGMLPKNRLGDKIINNLYVFAGAEHNMQAQKPKQIDLNALK"
     gene            complement(11186..11791)
                     /locus_tag="PKH68_02625"
     CDS             complement(11186..11791)
                     /locus_tag="PKH68_02625"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX11862.1"
                     /translation="MLIKIYNDNPNPNQIRHVAEVLRNGGIIIIPTDTVYAFGCDIFN
                     SAAVEFISRLKNKDLRKANLSFICHEMSQISEYAKLDDDSFKLMKKNLPGPYTFILNG
                     SNRLPKLFKNKKTVGIRMPDNRITLQIVRELGNPMMTSSIFIDDETTEYITDPELIDE
                     KYGNQVDLVVDGGMGGLLHSTVIDCTGDEPEIVRQGSHDLI"
     gene            complement(11889..12644)
                     /gene="tpiA"
                     /locus_tag="PKH68_02630"
     CDS             complement(11889..12644)
                     /gene="tpiA"
                     /locus_tag="PKH68_02630"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNX11863.1"
                     /translation="MRKNIVAGNWKMNKTLQEGVALATELKELLAGQTPNCEVVIGVP
                     FIHLATVAGMVEGSAIQVAAQNCANKESGAYTGEVSASMVKSTGATHVILGHSERREY
                     YGETSAILNEKVALAFANGLTPIYCCGEALEVRNANTQNEFVKTQLEETIFNLSAEDF
                     SKLAIAYEPIWAIGTGVTASTEQAQEMLAFIRSLIAARYGAEMAENTSILYGGSCNAK
                     NAPELFAQPDIDGGLIGGASLKAADFKAIIDAF"
     gene            complement(12801..13952)
                     /locus_tag="PKH68_02635"
     CDS             complement(12801..13952)
                     /locus_tag="PKH68_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX11864.1"
                     /translation="MYQQHQRARSANKPATTTLQKATKILLEIARILVGLTFVFSGFV
                     KAIDPLGSTYKFEDYFTAFGGFFEHLTPIAFPAAIALSTLELLIGICFLLKIKIKVMS
                     VVALLFMAVMTPLTLYIALKNPVTDCGCFGDAIILGNWTTFYKNLVLVALILLVLIFN
                     RQFRNLFMPKVEWAIVVLTAIVGVGLSVHGFRHQPMIDFRPYKVGVNIPEKMVVPEGA
                     AGDKYETTLIYQKNGQQQEFTIENYPKDSTWTFVEQKTTLVSKGFTPPIHNFSIVDQK
                     LNDITEYVLTYKGTVNLLCMYDLSKTSLKGAEAAETLYQKIKASGAPFYALTASSDEE
                     IDAFRSKTGATYPFCKTDPITLKTIVRGNPAMMTIKNGTIIGKWNWRDF"
     gene            complement(13984..14526)
                     /locus_tag="PKH68_02640"
     CDS             complement(13984..14526)
                     /locus_tag="PKH68_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNX11865.1"
                     /translation="MNTTSQQFDQVTAICRDIFTKKLKDYGSSWRILRPRSVTDQIFI
                     KANRIRSIEIKGVNKVGEDIRGELIAIVNYSIIGLIQLELGFADVDDLSVEDALTLYN
                     KYIELAKSLMAAKNHDYDEAWRSMRAESYTDLILQKIYRTKQIEDHEGKTLISEGIDA
                     NYFDMINYAVFGLIKIEFDN"
     gene            complement(14599..16029)
                     /locus_tag="PKH68_02645"
     CDS             complement(14599..16029)
                     /locus_tag="PKH68_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNX11866.1"
                     /translation="MASLAELFEKQTGTKPISEVQLTVSGSNRKYYRLANKKISLIGV
                     VGTSIEENRAFIAMALHFRKKGLPVPELGEVSDDEMMYLQEDLGDTVLFDYLVNSRKT
                     GVFSSEEKVFLDKTMEMLARFQVVGAQGFDFSVCYPLPEFNERSILWDLNYFKYCFLK
                     STGLDFQENRLEDDFVKLAQELLCAKTDTFMYRDFQSRNVMMKDNEPYFIDFQGGRRG
                     PLYYDVASFIWQAKAQYGPALREELVDAYLSSLKKYMEVDEAEFRRVLDHFVLFRLLQ
                     VLGAYGFRGYFEKKPHFLQSIPYALENLRALMKNNFKEYPYLMKVLREMSELKQFKDV
                     RLHRPLVVTVYSFSYKKGIPHDVSGNGGGFVFDCRAVNNPGKYERFYSMTGLDEPVIQ
                     FLENDGEILKFLSSAYSLVDASVKRYKDRGFTSLMVSFGCTGGQHRSVYSAQKMAEHI
                     SEKFGVEVNLIHRERNLEQTFEEVKE"
     gene            16383..18116
                     /gene="lysS"
                     /locus_tag="PKH68_02650"
     CDS             16383..18116
                     /gene="lysS"
                     /locus_tag="PKH68_02650"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNX11867.1"
                     /translation="MSIELSEQELFRRQSLTELRNMGIEPYPAAEYPTNAFSTDIKTE
                     FKDEDTRRQVCVAGRIMSRRIMGKASFVELQDSKGRIQVYVSRDDINTEAQPEMYNTV
                     FKKLLDIGDFIGIKGFVFRTQMGEISVHAQEMTVLSKSLKPLPIVKYKDGVAYDAFED
                     PEQRYRQRYVDLVVNEGVKDIFIKRTKVYQSMRNFFNAQDYIEVETPILQSIPGGAAA
                     RPFITHHNALDIPLYLRIADELYLKRLIVGGFEGVYEFSKNFRNEGMDRTHNPEFTAM
                     EIYVAYKDYNWMMTFTENMLEKICLDVNGTTEVKVGDNIINFKTPYKRISMIDSIKEF
                     TGIDINGMNEEQLFEVCKKLNVDVNNTMGKGKLIDEIFGAKCEGNYIQPTFITDYPRE
                     MSPLCKRHRENPELTERFELMVNGKELANAYSELNDPIDQLERFQEQLQLGEKGDDEA
                     MFIDLDFVRSLEYGMPPTSGMGIGMDRLVMLMTGQQAIQEVLFFPQMKPEKAISQDTN
                     EKYIALGIPAEWVDVVKKAGITKVRDLSGLNPNKFHQDICGLNKKNKLDLKNPTKEEV
                     QAWIAAAGDLA"
     gene            18220..19293
                     /locus_tag="PKH68_02655"
     CDS             18220..19293
                     /locus_tag="PKH68_02655"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNX11868.1"
                     /translation="MITESIEWGNTNISLTDILENNPPKKHVDKVRIAIMGGGSWATA
                     LAKIALTNVRSINWYMRRQEQIDEFIRLSKNPSYLTGVKFDTDRIKFTNDINKVVKTS
                     DILVFATPSPFLKQHLTKLKRRLDDKIIVSAIKGIVPDENMLISEYFHEAYKVPEANI
                     VALGGPCHAEEVALERLSYLTIACKSRDNARYIAEKFTNSYIHSRISDDVYGLEYTSV
                     MKNIYAIASGICSGLKYGDNFQAVLISNAVQEMNRFCNATNPQHRDISESAFLGDLLV
                     TAYSKFSRNRLFGTMIGKGYSVKTAQLEMEMIAEGYYATKTIHELNEKYQVNIPIVNA
                     IYEILYNRSSPILEIKELTEKLQ"
BASE COUNT         5395 a         4171 c         3879 g         5929 t
ORIGIN      
        1 acagctatta cacccgctat tccggtgaaa taaatcccga taaaagtgag atttcagaag
       61 gccgatattg gaaaccggag gagattaaat cggctatagg aaagaatatt tttacaccca
      121 attttgaatc cgagtttcca aaattaatcg aagtcctgat acaaaaaaat aactgatttt
      181 taaaataaca aaagcacctg aataatcaga tgctttttat aatttgtgat ttattatttt
      241 atgaaatcag caagtgaccg aatgcaaaat aaataattgc tgcaataagc gcacttaccg
      301 gtattgtaag tatccaggca accaacaact gctgtgtcac tccccagcga actgcggata
      361 agcgttttac tgaacctacg ccaataattg ctcctgtaat tgtatgtgtg gtacttaccg
      421 gaattttcag gaactcagtc agataaaggg ttaatgcacc tgctgtttcg gctacaacgc
      481 cttcgagtgg agtaactttg gtaatgcggg tacccattgt ttttacaatt ttccagccac
      541 cggacatggt gccaagagag attgcagtaa aacacgcaaa agcaacccag ttgggtaatt
      601 gtgctacgga ttcaatgccg tggcctattt ccggatgatg aatgtgggtt gcaatcaaag
      661 ctgccgcgat aatacccatt actttctggg aatcattcaa accgtgtccg atactgaata
      721 aaccggaaga cactaattgt aagtgtttga accattcatt tgctttgtga ggatttgccc
      781 ttcgaaacgt ccagtagaca aaaagtgtaa gtagggctgc cactgtcata ccgattagag
      841 gagctaaaaa gatgaaagag gctattttca gaattacagc accttttaca acggcaaagt
      901 tacccatatt ggccgcaatt gctgcaccgg caaaaccacc gatcagcgta tgcgaggagg
      961 aagatggtat tcccagccac cacgtagcca gattccagat aatggcagcc acaatcccgg
     1021 agaaaataat ggggagtgta acgtattctg cgattactgt cttggaaacc gtatcagcaa
     1081 ttccaaatcc gccgataatg tattttgcaa taaaaaatgc cagaaagttg aaaaatgcgg
     1141 cccacagtac ggcctgaaaa ggtgtcagca ctttggtgga tacgatggtg gcaatggaat
     1201 tggcggcatc gtgaaacccg ttgataaaat caaatatcag tgccagtaca atgattacta
     1261 ttaataatgt cattcttgta tttttgttaa taaaaaaaat aatgcataaa tgtcggatta
     1321 aaaattatcc tgatacttat gcatatttaa caataatggt ttttataatt ttacctacgt
     1381 gttcggcagc atcggtagct ttttcaagct catacataat ttctttctgt ttgataagtt
     1441 caacgccgtc ttttgcattt tcaaaaagat caatcagaaa atgttcataa acatcgtcag
     1501 ctttattttc aattagatga agttcatagc aatactcctt gatgtttttt gcatttttct
     1561 tcagtacatc cagctcatcc acggcttttt ccaacataag gcttccttcc tttatcaact
     1621 gtacaagttc gttagctgaa ggtggaagaa cttttggatt ataaagaaaa attcttttag
     1681 ctgcgctgtt gatatagtca gttacatcat ccagatgagt agccagatta ttgatatctt
     1741 ccctgtcgaa cggtgttata aaggttttat tcaattcgtc aaatatctta tttgacaaag
     1801 catctccttt tcgttcttct tctttaatct tccgatagta ctcagcggca ttttcatgat
     1861 ttcctttttc aatgaattcg atcatgatgt ctgtagcgtc ttttattaca tctaccattg
     1921 ctttcaatag tggaaagaac ttgggttctc tgggtgtaaa tttactaaaa aatgaattca
     1981 acattgttct tttaaattta gttgttattt ttttatttgt ttaattttca attatttatc
     2041 cttgaaacat ttcgaaaaga attcagatag ttcttttttt aatattttta caaaattaca
     2101 aaaaacgttt caaatgaaat gtaaataaaa cacatattta acggaaatgt aacattattc
     2161 taatcatttg ataatctgat ctttaaactt tcagctatta tgtattttaa aaaaaacaag
     2221 aaatgctgat ccacaatgac tatctcttcc cggtatgaaa actgtaaaag tacttatctt
     2281 aaataattat ttcataaaat gaagataaaa cttttttagg tatagaattt gttttacatt
     2341 tgcagaacta attagtacta acaaaaattt ttattaaaat ggcttacgta attacagaag
     2401 attgtattgc atgtggcact tgcattagcg aatgtccggt tgaagcaatt tcagaaggtg
     2461 atatctatgt tatcgaccct gatgtatgta cagattgcgg cacttgtgct gatgtttgtc
     2521 cttcagaagc aattcatcca gcgtaacaca atacgctaaa aatgcaaatc ctattcgtcc
     2581 ccgtgacgga taggattttt ttttgtttca ataagctata atttcactgg cagctatttt
     2641 ggcggcctga ccattgccat ataaattcag agaaaagtct gatttcttat tcacactcca
     2701 attgaatgct tcggatattt tacggctatc acttccggtg agtacattat aactgttatc
     2761 caccagttcc acccattcgg tctcattgcg cagcgtaata cattgcttac cgaagaagaa
     2821 agcttctttt tgcaccccgc cactatcggt aacgaccatt ttgcagtttt tgagcagcaa
     2881 aatcatatca aaatatccga caggttcaat gactgtaaat tcaggaacga ttttttgagc
     2941 gttgaggata ttccgtgttc gcggatgtaa cggcactatg actgtaattt gtttatttat
     3001 agcatttagt ccgtcaatta ttcccttcag attttggggt gagtcggtat tttcctgtcg
     3061 atgaaatgtt gccaacacaa attccggtga atttagttta gacaaaatga cagatttttg
     3121 ttctgccttc tgcacataat aaagtgcagc atcctgcatc acatcgccat tttttactat
     3181 tttgcaacta taattttcaa aaccctctct tttcaggtta ttgacagcgg tgtcggttgg
     3241 gcaaagcagc aagttggaaa tccggtcggt gagaatgcgg ttgatttcct cgggcatagc
     3301 catattgaaa gagcgcaagc ccgcttccac atgcaccaga ggtatatgca gtttagctgc
     3361 tgccagtgct cctgctattg ttgagtttgt atctccgtat actaagactc cttcgggttt
     3421 ttctttcaac aggatttctt ctatttcttc gagcatacgt ccggtcatgg ctccgtgacc
     3481 tacaccgtga atgtccaggt tgtacgcagg ttttggaatt tccatttctt caaaaaaaac
     3541 ttccgacata ttggcatcga aatgctgacc ggtgtgaata attttctcgt caacacccat
     3601 caatccaaac agccggctca aggtggctgc tttaacaaac tgaggtcggg caccgacaat
     3661 ggtaattatt cgtttcatag attttttatg atttcaatga ttcgctcggc agtgtgacca
     3721 tcccaaaatt tgggattctg acctgtttta ccattaccac gtagaatttc ggaaacttcc
     3781 ggtacaattt cttctgtttt aattaattta ttggagcctt cccagattgt taccggtctt
     3841 tcggtattgg gccgcacggt gaggcaggga atattcaggt gagtggtttc ttcctgtatg
     3901 ccacccgaat cggtcagggc aaaccgggcg ttgctcacca gatgaataaa ttcaaggtaa
     3961 ccgagtggtt ctattaagaa gacattgctc gtattcaact tatccattaa gccaaacttt
     4021 ctgatattac ccaatgtacg gggatgaatg ggaaaaatga gtggaatact ttttgaaatg
     4081 cggcaccaga tatcgacaag taattccagc gattcttttt catccacgtt ggacggacga
     4141 tgaaatgtca taactccata tccgttttct gaaaaatcgt tgatatcaat tttatcggtt
     4201 ttattcagaa gaggaaaact ctgaggagaa ttctgagctt ttctcaggtt ggcttccagg
     4261 gtatcaatca tcagattgcc caccataaat atttttttag gatcaactcc ctcgcgcatg
     4321 aggttttcat cagcatcggc actggtagtg aaaaaataat cactgacagc atcggttaca
     4381 aggcggttta tttcttcagg catggtgcgg tcattgctcc gcaagcccgc ttcatagtga
     4441 attgttttaa tattcagttt tgcagctacc agcgtgcagg ccagtgtact gttcacatcg
     4501 cccaccacca ttacgaaatc aggcttttcg tccagacaaa cttgctcaaa gccgatcatt
     4561 atttttgccg tttgtacggc atgagaggcc gaccccactt ccagattgat atccggtgcc
     4621 ggaatgccaa gttcttcgaa aaaacggccc gacatctgtg tgtcataatg ctggcctgta
     4681 tgtactaaaa ccggttgaat ttctttatca tccttcaatt gatgcatcac aggagctatc
     4741 ttcataaaat tgggacgggc tcccacaacc agtattaatt tcttcatttc tgttggtttg
     4801 acttagttgg taatattatc taaaagttga gccattatcc cacattgcgc ttttctggaa
     4861 tattgctcat aattttcagc tgttgattgc agccgattgt ttttatattt ttcaaatagt
     4921 tcgagaataa cctttttcat ggcatcgaca tcttcaaaat caacaatact tcccgatttg
     4981 gtttctttaa gtaaattagc tatatcactt tcaggaggcc caatgcataa aaccggacga
     5041 cgggaagcaa tatattcata taactttccg gttaatattc cattggcatt aggggtgtta
     5101 ttgatgagca gaagtaaaat ctgagatgtt tgctgaaatt caacagcttc ctgatgcgat
     5161 aaatatccaa ccttttcgac aaagccagtc aattgatatt tttctaaatc ggcgaacaca
     5221 accggttcta catttccaac cagttttatc cttaaactcc ttctgaattc ttcattctct
     5281 tcgagcaaca ggcttaatgc aacccacaat atttcaggat tacggtcact gtttatactt
     5341 ccgatgtgtg aaatagagaa ttcggcatct acggatttat tcgttgggat tacatcttct
     5401 tcatcgtaac cgttatgaac taaatctacc gttcgtcctg caatttctgc caactcacgg
     5461 caccaggtag gtgtaacact caccacttta tcggcatttg aaagtacctt tttttcgtga
     5521 tgatgatgta ttttgtcggc cacccaactc aggttgagtt ctcggtaaaa atcaatattt
     5581 gtccagggat cacgaaaatc ggctaaccag gggatatgaa cttgtttgtg taatttaagt
     5641 ccaatcatgt gcatggaatg cggcggaccg gtggatatta tagcatcgac cggattttcc
     5701 ttcaggtatt ttttaagata tcgaacagaa ggattaatcc agaagcaacg gggatcggga
     5761 attaaaaaat tacccctgac agacatcgag aacttatgta tcagactttt tttcttgccg
     5821 ccttccgaaa atgatacgtt aactttctga cctcttttac ctatgagcgt tttataaatt
     5881 tcgtacggtt cccatatcgg tctttttatc actaccaaac cgggtgaaat atctttttgc
     5941 aaactttggt cttcgatagc aaaatcggga ttttccggcg tgtaaataat cggctcccaa
     6001 tcatactttg gaaggtattt ggagaatttc aaccagcgct gcacaccggc accacccaat
     6061 ggtggccagt aataagtgat tatcaggact ttcttcatgt tggaaatttc caatttacct
     6121 ttaactcaat gatcattatt cttcatcctg tttgctcaaa aagctgatag cacgttgaag
     6181 ccgaacaact gtttcttcct ttccgataat ttcggtaata tcaaacaggt ggggaccgct
     6241 ggcagcacca actatagcca ctctgaatgc attcagaata gctcccattc cataaccatt
     6301 gtcctcaatc catttgcaaa taaccgcttc ctgattagct gccgaaaaat catccatggt
     6361 ttgaattact tcagtcagct cggtcaggcg ctcgggcgag tcctgtttcc agcgtttttt
     6421 taccgacttc tcatcgtact cggcaggtcc cctgaagaaa taaaaactct gatcccacag
     6481 gtcggtaacg aaattggcac gttctttaat aagccccact accctgctta ctttttcgga
     6541 gtcttctata atatcttttt caaccagaat atgctgaaaa atatcggcca gttcttcatt
     6601 ggatttcatt tgcaggtatt tatggttgaa ccatttacct ttttcgtagt caaattttgc
     6661 accactttta cttacccggt ccagcgaaaa ttgttcgatc agttccttca tgctgaataa
     6721 ttcctgctct gttccgggat tccagccaag caatgccaga aaattaacaa ccgcttcggg
     6781 gaaataacca gattcccgat atcccgatga tactgtttcg gtatttggat cttcccactg
     6841 gaggggaaat accggaaatc caagccggtc gccatcgcgt ttactcagct tgccatttcc
     6901 atcgggtttg agcagcaatg gtaagtgtgc aaactgcggc atcactgctt cccaacccag
     6961 atatctgtaa aggagtacat gcaagggtgc tgaaggaagc cattcttcac cgcgtataac
     7021 gtgcgatatt tccatcagat aatcatccac cacatttgcc aggtgatacg taggcaattc
     7081 atctattgat ttgtagagta ctttatcgtc caataccgag gaattgatta tcacttcacc
     7141 gcgaatgagg tcattgacat gaatttctat gttcggttca atttttattc tcactacata
     7201 ctgatcgcct tccgacaaac gtctttcaac ttcttctttt gtcaaggtca atgaattttg
     7261 catcatatca cgtgtcgaag catcgtattg gaaattggca attttactgc gctgaacatc
     7321 gagttcctgt ggtgtatcaa atgccatgta tgctttgccg tcggcaagca attgttcgac
     7381 atattttttg taaatttctt tccgttcgct ctgccggtat ggggcatgtg ctccttcctt
     7441 atcggcacca atgccttcgt caatttcaac accgagccag cggagtgctt ccaccacata
     7501 ttcttcagct ccgggcacaa aacgggcaga atcggtgtcc tctatccgta gtagcatatc
     7561 gccaccgtgc tgacgggcaa ataaataatt atacaaggct gttcgtacac ctccaatgtg
     7621 taaaggaccg gtaggacttg gtgcaaaacg cacccttaca tttctttcca ttttaattat
     7681 taatgatgaa gatacaaaat tacagaaaag tttatgaaat tcaaaagatt aaaaatttca
     7741 aatcttccta gcggttcaaa ggtttataaa taaaagtgaa attcaacaaa aaacctcccg
     7801 gaattccggg aggtttttgc tttattataa tctttataag attattcctg atttaatgtt
     7861 actcgtttaa aaccggtgca ggttaaatgt ttttttgcaa gcagttcttt tactgttgtt
     7921 tttccggctt cttctccacc accctcgtac ttttgttcta caagagtata ttctttgaag
     7981 aattttgcca gacgtcctgc ggcaatactg tttactttct gttcaggcag agaagcagac
     8041 ttttctgcag taacagtttc ctttatttcg cgggcttttg cagcatcttc tgcggtgatc
     8101 cagccttttg ccatattcga ttcaatgtgg tcttcgctat caacatgagc ggggttgatg
     8161 ccggcttttt tcaaggcaac ttccacttcc tttgcaacaa gttcagtttt ggttttttcg
     8221 atagccactg ccagctcgtt atcttttacc ttttgaggta cagccgattc atcaatagcc
     8281 acaggcgaca ttgctgcaat atgcattgct actccgtgta tggtttcata ttcggcgtca
     8341 ttttcagcaa aggcaacgat agtagccaat ttgttgcccg gatgaatgta agcagttacc
     8401 gtgccgcctt ttacaaattc atagtagtcc aactccagtt tttcgccggt aattccggaa
     8461 cgttcggtta caagatctcc gattgtacgt ccgtcatcag taatagttat agctttaagt
     8521 gcttctaaac tttcaggttt ttcagctaat gccttatcga gtattgattg tgttaatgcg
     8581 ataaaatcag cattttttgc tacaaagtca gtttcacatt ttaatgccaa aacagctcca
     8641 aatccatctt ttgtgtctgc aagcacacaa ccttcagaag cttcacggtc gctgcgtttt
     8701 gctgctacag cctgtccttt tttacgtata atttctactg ctttttcaaa atcgttttcg
     8761 gcttcgatga gtgcattttt gcaatccatc atgccggcac cggtcatggt gcgcaatttt
     8821 gtaatttctg ccattgttac tgccataata tctttaattt aattactacc cccaacccct
     8881 aaaggggagt tatttgagag tctgtgttat tttaatttat taatttaatc tgaaatcgaa
     8941 aaaaaacagg ttacaagcta caaaaaatca taacccgcaa cctttttttt ggtcccgaca
     9001 ttccccttta ggggttaggg gtctttattc ttcttctttt acgaattttt ttacaacgtt
     9061 tgctttcagg gcttcgtcgt catctttctg agttctgggt cttttcgaaa cacgtgattt
     9121 cttttccttc atctcaggag cttctgatgc ttcagcgtcg attttttcaa ccttacgttc
     9181 ttccagacct tcctgaatgg cagcacacat ggcgttgagg ataacttcaa ctgattttgt
     9241 ggcgtcatca ttggccggaa ttacgaaatc gatatcattt ggattggagt tggtatcaac
     9301 tataccaaac accggaatac ccagacgttg tgcttccttc actgcgatat tttctttcat
     9361 cacatcaact acaaaaagtg cggaaggcag acgtgtaagg tcggcgattg aaccaaggtt
     9421 cttttccagt ttttcgcgct gacgggtaat ctgaagtttt tcacgtttgg agatattatc
     9481 aaatgttcca tcggttgaca ttttgtcgat ggtagtcatc tttttcacag ccttacggat
     9541 agtcgggaaa ttggtcaaca taccacctgc ccagcgttct gtaatgtatg gcatacctac
     9601 cgatgtagct ttatcggcta ctacgtcttt tgcctgtttt ttggttgcta cgaataatat
     9661 tttacggccc gatttagcta tttgtttcat tgcagcggct gcttcgtcaa ttttcaccac
     9721 cgttttgtgc aaatcaatga tatgaatatc attgcgttcc ataaagatat atggagccat
     9781 tgcaggattc catttacgtt tcaggtgacc aaagtggcaa ccggcttcta ataattcttc
     9841 gaaatttgtt cttgacattt ttattttttt atttgtttac attctgttga aaatcaatcg
     9901 ttgagtagcc tttcagctca cagccgtcag cttgctgctt gcagccgtca gccattcaga
     9961 gtctccccga tttagatact aaacttaaaa ccattgacga tttattcatt taccatttac
    10021 tattggcgtt tacgccgcag aataaaattg taaattgtaa atcgaaaaat ggtaaatatt
    10081 tttttaacgt tttgagaatt ggaatctctt gcgtgctttt ggacgtcccg gttttttgcg
    10141 ttccacttcg cgtgggtcgc gggtcataaa tccttcggat tttaatgccg gtttgctttc
    10201 aggattaatt ttcaccaaag cgcgggctat tgccagacgc aatgcttctg cctgtccttt
    10261 gtatccgcca ccatcaagat tcacttttat atcgtatttt tctacaacac caattttatt
    10321 cagcggttgt tttacaacat attgcagtat aggtgatggg aaataaacgg caagatctct
    10381 tttattgata gttatctgtc cttttccttc acttacgaaa acgcgagcaa tagctgcttt
    10441 tcttcttcct aatgcattta ctacttccat atcggttatt taagtgcgtt taaatcaatt
    10501 tgtttcggtt tctgagcttg catattatgt tcggcaccgg caaacacata aaggttgttg
    10561 atgattttgt caccaagacg atttttgggc aacatgcctc ttaccacttt acgaatcaaa
    10621 cgatccggat cttttgccat taaatcggaa ggagtatatt ctctttgtcc acccggaaaa
    10681 ccggtgtgac gaaggtatat gcgatcagtc catttgttac cggttaattt cactttttcg
    10741 gcattgataa cgattacatt gtctccacaa tccacatgag gagtaaaact tggtttgtat
    10801 tttccgcgca aaagttttgc aacttttgag cccatacgtc ccaataccag atcagtagca
    10861 tctactacca cccattcttt ctgcacggtt gctttattgg cagaaatggt tttataactt
    10921 aacgtatcca cttttataat tgtttttata attaatattc agtctgtttc ctgacgacat
    10981 atcggctaca tgagagaggc taatcggcac attataggat atttaactcg tcggatgcgg
    11041 actatttcac cgggataata aacggactgc aaaaatacag tttttgtttt gattggcaaa
    11101 ataatttgaa aagatttttt tatagccttt acagctatcc ctgagggctt tcaatgatga
    11161 gagaaatgat tattctaaaa tccggtcaga tcagatcgtg agaaccctga cgcacaattt
    11221 ccggttcgtc gcccgtgcaa tcgataacgg tagaatgaag cagaccgccc ataccgccat
    11281 ctacaaccag gtcaacctga ttaccgtatt tttcatcaat cagttcggga tcggtgatat
    11341 actcggtggt ttcgtcgtcg ataaatatgg agctggtcat cattggattg ccaagttcgc
    11401 ggactatttg gagcgtaatg cggttgtcgg gcatccgaat gccaaccgtt ttcttatttt
    11461 tgaaaagttt gggaagccgg ttactgccat tgaggataaa ggtgtatggt ccgggcagat
    11521 tttttttcat cagtttgaag gaatcatcat ccagtttggc atattcgctt atctggctca
    11581 tttcgtgaca gataaatgaa agatttgctt tgcgaagatc tttatttttc aatcgggaaa
    11641 taaattccac ggctgccgag ttgaaaatat cacaaccgaa agcgtaaacc gtgtcggtag
    11701 gtataataat aatacctccg ttgcgcagaa cttcggcaac gtgacgaatc tgattggggt
    11761 taggattatc gttatagatc tttatcaaca tggttttcgt tttttcaaac cggactgcaa
    11821 aaatacaaaa taatcctgca aacaaaagtt tgcaggatta tttatttgat tttttctttc
    11881 aattgaaatt aaaaggcatc aataattgcc ttgaaatcag ctgctttgag cgatgctcca
    11941 cctatcagcc cgccatcaat atcgggttgt gcaaataatt ctggcgcatt tttggcatta
    12001 caacttcctc cgtacagaat ggatgtgttt tcagccattt ctgcaccata tctggcagca
    12061 atgaggctac ggatgaatgc cagcatttcc tgagcttgtt cggtggatgc agttacaccc
    12121 gttccgatag cccagatagg ttcgtaggct atggcgagtt tactgaaatc ttcagcgctc
    12181 aggttgaaaa tggtctcttc gagctgagtt tttacgaatt cgttttgagt attggcgtta
    12241 cgtacttcaa gcgcttcgcc gcagcaatag atgggggtaa gtccgttggc aaaagccagc
    12301 gctacttttt cattcaatat tgcgcttgtt tctccgtaat attcgcggcg ttcggagtgt
    12361 cccagaatta catgggtagc accggttgat ttcaccatgg aagcacttac ttcaccggtg
    12421 taggctccgg actctttatt cgcacagttt tgggctgcaa cctgaatagc ggaaccttca
    12481 accattccgg ctaccgtagc caggtggata aaaggtacac cgattaccac ttcacaattt
    12541 ggtgtttgac cagccaagag ttcttttaat tccgtagcta aagctacacc ttcttgcagg
    12601 gttttgttca ttttccagtt acctgctaca atgttttttc tcataatgat gaatgtacct
    12661 ctaaccccta aaggggaaag atggattttt tatttttaag ttattattaa tattatttcc
    12721 gacatataaa tcaatgtaca aaacatgttc aattaagcta aattagcatc aaattcccct
    12781 ttaggggtta ggggcagggg ctaaaagtct ctccagttcc atttgccgat tatcgttccg
    12841 tttttaatcg tcatcattgc agggttacca cgaacaatcg ttttgagtgt aatcggatcc
    12901 gttttgcaga atggataagt cgctccggtt tttgaacgaa aagcgtcgat ttcttcgtcg
    12961 gacgaggctg taagtgcata gaatggtgca ccgctggctt ttatcttctg ataaagtgtt
    13021 tcagcagctt cggcaccttt cagtgacgtt ttgctcaggt catacataca aagcagattg
    13081 actgtacctt tgtatgtgag cacatattcg gtgatatcat tcagtttctg atccactatg
    13141 ctgaaattgt gtataggcgg tgtaaatcct ttggaaacca gcgtggtttt ttgttctacg
    13201 aatgtccatg tactgtcttt cggatagttt tcgatggtaa attcctgctg ctgtccgttc
    13261 ttttggtaaa taagtgtggt ttcgtattta tcacctgcag caccttcggg aacaaccatt
    13321 ttttcgggga tattcacgcc cactttgtag ggtcggaaat caatcatcgg ctggtgacga
    13381 aaaccgtgaa ccgacaaacc tactccaaca attgctgtca aaacaacgat tgcccattcc
    13441 actttcggca taaacagatt tctaaattgc ctgttgaaaa tcaataccaa taatataagt
    13501 gcaaccagca ccaggttttt gtagaatgtg gtccagttac ccagaatgat ggcatcgcca
    13561 aagcaaccgc aatcggttac cggatttttc agcgcaatat agagcgttag cggggtcatc
    13621 actgccatga agagcaatgc aactacagac atgaccttaa tctttatttt caacaagaaa
    13681 catattccta tcaaaagttc cagtgtggaa agggcaatgg ctgccgggaa tgcgatgggt
    13741 gtgagatgct caaagaaccc tccgaaagcg gtgaagtaat cctcaaattt gtaggtagaa
    13801 cccagcggat caatggcctt tacaaatccg gagaaaacaa acgtaagacc gacaagaata
    13861 cgggctatct caagaaggat ttttgttgct ttttgaagtg tggttgttgc cggtttattg
    13921 gccgagcgtg ctctttgatg ttgctgatac atttttttct ttattttacc gtttggatgt
    13981 aaattaattg tcaaattcta ttttaatcag accaaaaacg gcgtaattta tcatatcgaa
    14041 ataattggcg tcaatgcctt cggaaatgag agttttgccc tcatgatctt ctatttgctt
    14101 ggtgcggtag attttctgca gaattaaatc ggtatatgat tctgcccgca tgctgcgcca
    14161 ggcttcgtcg tagtcgtggt ttttggcagc catcagactt ttggccagtt caatatactt
    14221 attgtacaat gtcagcgcat cttcaacgct caggtcatcc acatcggcaa acccgagttc
    14281 gagctgaata agtccgatga tgctgtaatt gacaatggca atcagttcac cgcgtatatc
    14341 ttcgcccact ttgttcaccc ctttaatttc gatactgcgg atacggttgg ctttgatgaa
    14401 gatctgatcg gtcacggagc ggggacgcag aatacgccat gaagaaccat aatccttcag
    14461 ttttttggta aaaatgtcac ggcagatagc tgttacctga tcgaattgct ggctggtggt
    14521 attcatacgg agttgagtta atttttttaa ttaacgcgca aaattactaa ttttctgcga
    14581 aacagcaaaa ccggttattt actcttttac ctcttcgaag gtttgttcca ggtttcgctc
    14641 acggtgaata agattgactt ccaccccgaa tttttcgctg atatgctctg ccattttttg
    14701 ggccgaatat accgagcgat gctgtccgcc ggtacatccg aaacttacca tcagactggt
    14761 gaaaccgcgg tctttgtagc gcttcaccga tgcatcgacc agcgaatagg cactgctgag
    14821 gaacttcaga atttcaccgt cgttttccag aaactggatg acaggttcat cgaggccggt
    14881 catggaataa aaccgttcat atttaccggg attattcaca gcccggcagt caaacacaaa
    14941 accgccccca ttgcccgaca catcatgcgg aatacctttt ttgtacgaaa aactatacac
    15001 tgtcactacc agcggccgat gcagccgaac gtccttaaac tgcttgagtt cagacatttc
    15061 cctgagcacc ttcatcaggt acgggtattc tttgaaatta tttttcataa gtgcccgcag
    15121 gttttccagc gcataaggga tgctttggag gaaatgcggt tttttctcaa aatagcccct
    15181 gaaaccataa gcgccgagca cctgaagcaa tcggaaaagc acgaaatgat cgagtacgcg
    15241 tctgaattcg gcttcatcca cttccatgta ttttttcaga gaactgagat aggcgtcaac
    15301 caactcttcg cgcagggcag gcccgtattg cgcttttgcc tgccagataa atgaagccac
    15361 atcgtagtaa agaggtcctc ttcttcctcc ctgaaaatcg ataaaataag gctcgttgtc
    15421 cttcatcatc acgttgcggc tctgaaaatc gcgatacata aaggtatccg ttttggcgca
    15481 gagtaattcc tgtgcgagtt ttacaaaatc atcttccagc cggttttcct gaaaatcgag
    15541 accggtcgat ttcaggaagc aatacttgaa ataattcaaa tcccaaagta tagagcgttc
    15601 gttgaactcc ggcagcggat aacaaaccga gaaatcaaat ccctgggctc ccaccacctg
    15661 aaaccgtgcc agcatctcca tggttttatc cagaaaaact ttctcttcgg agctgaatac
    15721 acccgtctta cgactattaa ccaggtaatc gaacaatacc gtatcgccca ggtcttcctg
    15781 caggtacatc atttcatcgt cagacacctc gcccagttcg ggtacaggca gacctttttt
    15841 gcggaaatgc agggccatag caatgaaagc tctgttttcc tctatcgatg taccaacaac
    15901 tcctataagt gaaatctttt tattggcaag ccggtaatac ttgcgattgg aacccgatac
    15961 tgtcagttgc acttcggaga tgggttttgt tccggtctgt ttttcaaata gttctgctaa
    16021 tgatgccata gaatgaattt gaatataaaa tgaacaggaa atgtttctgc aaatttacaa
    16081 ataaaaaaac acatttcaca attcaatacg ggaccatttt accccgatag ttatattgtt
    16141 acttgtaagt gaatttgttc gcatgtgcta aatgcagtat taaatttgac tttaatttac
    16201 tacaaaacat tgtttttata cctgtatcac attttacaaa cacctcatta ttagataaaa
    16261 acctcagcaa ttaacaacta ttcattgtca attggcttaa aattcatcct ttttttgtag
    16321 ttttgcaaaa taatttgtaa tgtgcatgag aatgcattta aatgattgaa aaataacagc
    16381 atatgagtat agaattaagt gaacaggaac tgttcagacg tcagagtcta accgaattgc
    16441 gcaatatggg tatagagcct taccctgcag ccgaatatcc caccaacgct ttttccacag
    16501 atattaaaac tgagttcaaa gacgaagaca cccgtagaca ggtgtgtgtg gcaggtcgca
    16561 tcatgagccg ccgcatcatg ggtaaagcat cgttcgtgga gttgcaggac agcaaaggac
    16621 gtattcaggt gtatgtgagc cgtgatgata tcaacaccga agcgcagccc gaaatgtaca
    16681 acaccgtttt caaaaaattg cttgacatag gcgattttat tggcatcaag ggatttgtgt
    16741 ttcgcacaca gatgggcgaa atcagtgtac atgcacagga aatgaccgta ctgagcaaat
    16801 cgctgaaacc gctgcccatt gtaaaatata aggacggcgt agcctacgat gctttcgaag
    16861 accccgaaca acgctaccgt cagcggtatg tggacctggt ggtgaacgaa ggagtgaaag
    16921 acatcttcat caaacgcacc aaagtatatc agtccatgcg caattttttc aacgcacagg
    16981 attacattga agtagaaacc cccattctcc agtcaattcc cggcggtgca gcagcacgac
    17041 cgtttatcac acatcacaat gcactggata ttccgctgta tctgcgcata gccgacgagt
    17101 tgtatttgaa acgcctcatt gtgggtggtt tcgaaggtgt gtacgaattc tcaaaaaact
    17161 tccgcaacga agggatggac cgcacacaca atccggaatt taccgccatg gaaatttatg
    17221 tagcctacaa ggactacaac tggatgatga ccttcaccga aaatatgctc gaaaagatat
    17281 gcctggatgt gaacggaact accgaggtga aagtgggtga caatattatc aacttcaaaa
    17341 ccccttacaa acgcatctcc atgattgatt ccattaagga attcaccggc attgacatca
    17401 acggaatgaa cgaagagcaa ctatttgaag tctgcaagaa actgaatgtg gatgtgaaca
    17461 acaccatggg caaaggcaaa ctgatagatg aaattttcgg tgcaaaatgc gagggaaact
    17521 acatccaacc cacctttatt accgattatc cgcgtgagat gagcccgctc tgcaagcgcc
    17581 accgcgaaaa tcccgaactg accgaacgtt tcgaattgat ggtgaacgga aaagaactgg
    17641 ctaatgctta ctccgaactg aatgacccga tcgaccagct cgaacgcttt caggaacaac
    17701 tccagcttgg cgaaaaaggc gatgacgaag ccatgtttat agacctcgat tttgtacgct
    17761 cactggaata cggtatgccg ccaacttcgg gaatgggtat cggaatggac cggttggtaa
    17821 tgctaatgac cggtcagcag gctattcagg aagtattgtt tttcccgcaa atgaaacctg
    17881 aaaaagctat atcacaggat acaaacgaaa agtatattgc cctcggcatt ccggccgaat
    17941 gggtggatgt tgtaaaaaaa gcaggcatca ccaaagtgag agacctgagc ggccttaatc
    18001 cgaacaagtt tcatcaggat atatgcgggc tgaataaaaa gaataaactt gacctgaaaa
    18061 atccgaccaa agaagaagtg caggcctgga ttgcagcagc cggagactta gcgtaaatat
    18121 tcacgggaaa atcgcttgca gggaacatat tttccctgta aagcgtttaa ataaaacctc
    18181 cgtgagttaa aaaattttcc taacaacgac aaattagaaa tgataacaga aagtattgaa
    18241 tgggggaaca ccaatatctc cctgacagat atacttgaga ataatccccc gaaaaagcat
    18301 gttgacaaag tgcggattgc catcatggga ggaggaagct gggcaacggc actcgccaag
    18361 attgcactta cgaatgtaag gtccatcaat tggtacatgc gccgacagga gcagattgat
    18421 gaattcatcc ggttgagcaa aaacccctcg tacctcacag gagttaaatt tgataccgat
    18481 cgtatcaagt ttaccaacga tatcaataaa gtggtgaaaa catcggatat cctggttttt
    18541 gccaccccct cgcccttcct gaagcagcat ttgacaaaac taaaacggcg cctcgatgac
    18601 aaaatcattg tttcggccat caaaggaata gtacccgatg agaatatgct gatatccgag
    18661 tattttcatg aagcatacaa agtgcccgaa gcaaatattg tagcgctggg cggtccctgt
    18721 catgctgagg aagttgcatt ggaacgtctc tcctacctca ccattgcctg caagtcgcgc
    18781 gacaatgccc ggtacattgc cgaaaagttc accaacagct acatccattc ccgcatcagc
    18841 gacgatgtgt acggactgga atacacttcg gtgatgaaaa acatttatgc cattgcatcg
    18901 gggatttgca gcggactaaa gtatggtgat aactttcagg cggtacttat atccaatgct
    18961 gtgcaggaaa tgaaccgttt ctgcaatgcc accaatccgc aacaccgcga catcagcgaa
    19021 tctgctttcc tcggagactt actcgtaacg gcttattcca agttcagtcg caatcgcctc
    19081 tttggtacga tgatagggaa agggtattcg gttaaaacgg cacagcttga aatggaaatg
    19141 atagccgagg ggtattatgc caccaaaacc attcacgaac tgaatgaaaa ataccaggtc
    19201 aacataccca tagtgaacgc catctacgaa attctctaca accgcagttc acccatactg
    19261 gaaattaaag aacttacgga gaaattacaa taagaggccc ccgaaagcct gcctgccgca
    19321 ggcagggggg aattccggcg ctttaaatca acatctcaaa atcaggtttt tttg
//
LOCUS       DAOPZK010000024        19302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2965_length_19302_cov_24.226321, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000024 DAOPZK010000000
VERSION     DAOPZK010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19302
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2965_length_19302_cov_24.226321"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PKH68_02660"
     CDS             <1..675
                     /locus_tag="PKH68_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX11869.1"
                     /translation="GETFIGLLKALDNIDADVRYRISSVEPNLLTDEIIQFVADSRHI
                     MPHFHIPLQSGTNEILQLMKRRYTVELFAEKMATIKRILPDAFIGVDVIVGVRGETDE
                     LFEQSFEFIQSLDFSQLHVFTYSERANTKMLEIEHNVPVKERKRRSERLHELSEEKTA
                     AFYASQTGKTASVLWEAKKNEEMMHGFTENYVRVERPYDKKRVNTAETVTLGDWNEDK
                     TALKAI"
     gene            752..1507
                     /gene="hisF"
                     /locus_tag="PKH68_02665"
     CDS             752..1507
                     /gene="hisF"
                     /locus_tag="PKH68_02665"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNX11870.1"
                     /translation="MLAKRIIPCLDIKDGKTVKGINFLNITDVGDPVELGKRYAEMGA
                     DELVFLDITATNEKRKTLSELVTRIAKQINIPFTVGGGISSVEDVSVLLSCGADKISV
                     NTAAVRNPQLIKDLADNFGSQCVVLAVDTKCIESEWFVFLNGGRVPTEIKTLDWVKQA
                     VALGAGEILLTSMNHDGTKDGFALDITRAVSEAVNVPVIASGGAGTMEHFVDVFDAGK
                     ADAALAASIFHYQEIGIQELKHFLKEKGVEVRL"
     gene            complement(1581..2411)
                     /locus_tag="PKH68_02670"
     CDS             complement(1581..2411)
                     /locus_tag="PKH68_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNX11871.1"
                     /translation="MTNDINLTSQEWLDIIFEGKNKDYGAYTLRSDSPKRHAVAFIVV
                     AAAAVLTFLAASLINTYNQNKQKEKITDVVQMSDISMKNAEVPKENIEKRYEAPPPVE
                     LKSTIKFTAPVIMDDNKVNENNQITSQDELVGAKVEISTTTVIGTDEQFGKNIADLEK
                     DKLVVGEKAPDEEVHIVVEQPPSYPGGDAALMEYLNNNISYPTIAQEMNIQGKVTCSF
                     VVGKDGSIQDVRVERSVDGSLDKEAVRVIKSMPRWIPGRQGGNAVKVKYYLPVSFRLQ
                     "
     gene            complement(2688..4436)
                     /locus_tag="PKH68_02675"
     CDS             complement(2688..4436)
                     /locus_tag="PKH68_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11872.1"
                     /translation="MPKTDRLLKSGFYAGLILLLISLAACRPADEQQPAENLVELDSM
                     AYRYPKYIYNVVNNLIKQNQLTSYDRAYAGLILSIAADNMGRTLPSDSLISATVDVFA
                     QRSDYTPNNYVRALIYQGITRFHTGISDNRAYLPIKEALTLSELSRLDPKTRQKACYY
                     LGVIHTKNNNVSQAHEYFRQAYVIAENLKDSVILFKTCREMYWNRMKALDFLTARTLL
                     ETLQSFSNANKEQIRDIRNAEALYYNSNKRYRTALKIDYNLLRTDIQNKDSNALLADY
                     YRISDNYKYLNQPDSALHYGELATRNIRDTTFYLNYYYYLNVAELASQMKNWDKSTAA
                     YQQVYRLMNKTINNQLNTQILELERKYDLASAENRAMKLKNNNIILQLVLIILTMGLI
                     ITLLIFRNRSRLQKEQEKSLQQEKKILEQKQLIAEANEKQMMLDKQLAERKLVEKQFV
                     IPIYRQISQRNNAIKNFLLDLQSNSHIAKNPQILQKIETEYHDFVQTTNISEPQFLTD
                     ELFSGLTGISSEESRLFNESERMMMAFMATGSDNQQMATLMNTSVESIRVRKSKLKKK
                     MLENKVKIPEKMETEG"
     gene            complement(4469..4852)
                     /locus_tag="PKH68_02680"
     CDS             complement(4469..4852)
                     /locus_tag="PKH68_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HNX11873.1"
                     /translation="MRGKYLYEYAVIRLFPKVEREEFINIGIILFSKEANYIGMKYRS
                     DVEQTCRTATELDFGLLGDTLKSFAIICNGEKQGGKIAELEIAERFRWLTAERSTCIQ
                     TSRSHNGFTDNPENKLEELFAELVM"
     gene            complement(4830..5612)
                     /locus_tag="PKH68_02685"
     CDS             complement(4830..5612)
                     /locus_tag="PKH68_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HNX11874.1"
                     /translation="MNLRTVNVNRYITPLREGGSLPALAEADDGFRYVVKFSGSGHGT
                     KMLISELIGGAVAKILGLRIPELVYVQLDEAFGRSEGDEEIQDLLKASTGLNLGLHFL
                     SGALTYDPVTTKVDAALASKIVWMDAFLTNVDRTVKNTNMLMWHKELWLIDHGSTLYF
                     HHNWANREKQAANPFPLIKDHVLLEFASQLREADEECRNILNDEMLRDVVNLIPDEWA
                     HWNHDGETPEMIRDAYFKFLTDRLAQSEIFLNEAIHARKILV"
     gene            complement(5630..6073)
                     /locus_tag="PKH68_02690"
     CDS             complement(5630..6073)
                     /locus_tag="PKH68_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11875.1"
                     /translation="MKKSFPVLVLIITFLVTASTLAKAQLSVTSYSIYALGVSTDTQK
                     PFSVELKTFANSNFHNLGFELDAFHNFKTHEYHRFSVGLGLNSQPFNEIYLNEITVPL
                     VLEVFPLQSFKKLSLLVEFAPAMDVNIDDAGDLSFRHLWGIRYRF"
     gene            complement(6289..6951)
                     /locus_tag="PKH68_02695"
     CDS             complement(6289..6951)
                     /locus_tag="PKH68_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX11876.1"
                     /translation="MDILQTLDTLDKQWLLALNNDYPAFWDGLMYGISDKLTWIPFYV
                     ALIIILIKSLKKQSWLVILALIVCIIIADQVSSGLIKEMVHRLRPSRNPDLENMVCLL
                     KGYRGGKYGFVSSHAANSFGLAMLTSLFFRSRKYTIIIFLWATIVAYSRIYLGVHYPG
                     DVIGGAIVGVGAAMLVYWLFTKVLPDKIVFKKSAEINMSLPLWVLGASFVALIPYSLW
                     IY"
     gene            complement(7095..7856)
                     /locus_tag="PKH68_02700"
     CDS             complement(7095..7856)
                     /locus_tag="PKH68_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX11877.1"
                     /translation="MKRIYIIFCLTFLMFGQTVAQEKKSADKVALANQMYKQGQFTDA
                     ARIYEEELKKGVSAELYYNLANSYYKANEIGHAILNYERALRIDPGFDDASYNLQIAE
                     SKIVDNVNTSPTFFVRKWTNSLIKSYTSNQWAVISVVCFILMLALFFLFAFSRIRTRR
                     KYSFFASVLFGILCVVALSFSGIRKDQIKNHHEAVIMMGAVAVKSSPDQSGTDLFQLH
                     EGTLVKVKSVLGNWTEITLENGAVGWVEETAIERI"
     gene            complement(7858..9684)
                     /locus_tag="PKH68_02705"
     CDS             complement(7858..9684)
                     /locus_tag="PKH68_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNX11878.1"
                     /translation="MKRILIFFTSVLLTLTALSAQDKVSFTASAPATVYMDTPFQLVY
                     SVNASAKDLRAPDFQFFEILAGPFESHSSSYQNINGKATSSVNVSYTFTLLPNKVGTF
                     KIPGAAIEVDDEKYVSNAMTIKVLPADKTQSQGQGGAGSPATGAETQRISNDNIFIRT
                     SVSKTNVYEQEAILVTYKLYTLLDVAQFTEMKFPDFNGFLKQEIAQPKNAQLSYENYN
                     GKNFGTVVLYQVLLFPQRTGEINIDKANFTAILRIQNKAQVRSIFDDFFDSYSNVQKS
                     LTAPGARITVNALPAGKPASFTGAVGNFSLSSSISKTNLKANDAGIIKVVISGTGNMK
                     LLQNPEIKFPEGFEVYDPKADNKFSTNANGVSGTKTIEYMFIPQHAGVFNIPSAELSY
                     FDLNDRTYKTLRTPAYRLNVAKGVGGENTIVGNYTNKEDVTQMAKDIRYIHTGKIKLH
                     AEETPLFGSMLFWMMFLIPLLVAGILFIYFRKQIRENADISLMKTKKANKVAQKRLKL
                     AQKFLTEGKKSQFYEEVMKAVWTYLSDKLSIPVAALNKENIAAEMTERGIDNELTNQF
                     TNILNTCEYASYAPNSGQQEMGNLYEETIEAIGKVEEYFKKK"
     gene            complement(9756..10436)
                     /locus_tag="PKH68_02710"
     CDS             complement(9756..10436)
                     /locus_tag="PKH68_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX11879.1"
                     /translation="MKELKNILLIVAMLLPLVGYPQKESNDVRTGNRHYNNKKYTEAE
                     IEYRKALVKNNKSFEANYNLGNALFKQGKYSEALQQYKSAIPFAGNNKQKAASEFHNI
                     GNTLIGENKIQEAIDAYKTALKNKPEDNDTRYNLAYAQMLLKKQQQENKDNKDKQDKG
                     NDQQQKQPKNEPDKDKKQEQPQPQQPQMSKENAQQILEALQQDEKDTQDKAKKAQIQG
                     RKKAEKDW"
     gene            complement(10462..11505)
                     /locus_tag="PKH68_02715"
     CDS             complement(10462..11505)
                     /locus_tag="PKH68_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX11880.1"
                     /translation="MFRFAHPEYLFLLLPAVIPVAAFVRAAVLRKKNIKLWGNAALLA
                     TLMPNVSYARPRWKFYMQLVALILIIVVLAQPQFGTKEEKVRKKGIEVMIALDVSNSM
                     LAQDIQPNRLQKAKQILSQLIDKMEEDKVGMIVFAGDAYVQMPITSDYGSAKMFLSSI
                     NPQMVPRQGTAIGSAIDIAIKSFGQKSGVSRAIIVLTDGENHEDDAIAAAKLANDNGI
                     KIFVVGMGTPEGAPIPVGETVSFKKDKDGNVVVSKLNEQMCSGIAQAGGGVYVRSDNS
                     NAALRTVSRQLDALAKNEMDARVFSEYNEQFQSFAFIALFLMLIEFFVFSRKNKWLSR
                     IRLFDVKVQKIKA"
     gene            complement(11577..12560)
                     /locus_tag="PKH68_02720"
     CDS             complement(11577..12560)
                     /locus_tag="PKH68_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNX11881.1"
                     /translation="MTFLNPEYLFLLLLLLPMIIWYIREIHRSEASLQISSVQTLKDL
                     PRTRRMKFRHLPFILRLLAISMLILAIARPQSSNNWRTENTEGIDIMMALDISGTMQA
                     EDLRPSRLEAAKTVASEFILSRPNDNIGLVVFAGESFTQCPLTIDHGVLINLFSGIRY
                     GLIEDGTAIGLGLANAVSRIRDSKAKSKVVILLTDGSNNRGDIAPITAAEIAKTFGIR
                     VYAIGVGSYGVINIPIQTPFGVRYQQEETQFDEKTLKDIADITGGKYFRATNNNKLRA
                     IYQEIDQLEKTKISVKEFSNKSETYFIFALLAFLLFFAEILIRNTWMKTLP"
     gene            complement(12601..13488)
                     /locus_tag="PKH68_02725"
     CDS             complement(12601..13488)
                     /locus_tag="PKH68_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11882.1"
                     /translation="MDSTQLWIGNQTGFTFEILQAPGQKVTAPIFSDTLVGNLDIVEQ
                     AKMDTVKLSNDKIQVNIRYKLTSFQDSLVYVPAFPFVTGKDTAWSNSTSLKVVQPFVI
                     DTAANSLTDIKPVYKPPFDWKNFLQKLLLAILLLALAVILFVLVRKFVMKKPVFHTQT
                     PEEIIPANIEALRRLDKIKEEKIWQKGRVKEYHTELTDVLRTYIERVFNIPSMEMTSD
                     EILRSSQFLKVDKPAAFEGLKQILRLADLVKFAKWNPETVENELSLMNSYLFVNQTTV
                     EEIKSEESKADKTAEPETR"
     gene            complement(13607..14473)
                     /locus_tag="PKH68_02730"
     CDS             complement(13607..14473)
                     /locus_tag="PKH68_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNX11883.1"
                     /translation="METHELLKKVRKIEIKTRGLSQNIFAGQYHTAFKGRGMTFAEVR
                     EYQYGDDVRSIDWNVTARFGHPFIKVFEEERELTVMLLVDVSGSRDFGTGFMLKKDII
                     TEVAATLAFSTIQNNDKVGVIFFSDKIEKFIPPKKGKKHVLHIIRELLVFEPSSRQTD
                     IGGALKYFTNAIKKHSTAFVISDFMDHGFDRTLQIANRKHDIAALQVYDIREAEIPNV
                     GLIKLKDAETGERIWIDTADKRLRTSYKHKWNERQLELQKTFKQSGVDSVTMSTNEDY
                     VRALMKLFRLRA"
     gene            complement(14592..15587)
                     /locus_tag="PKH68_02735"
     CDS             complement(14592..15587)
                     /locus_tag="PKH68_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX11884.1"
                     /translation="MDQMVDIKELNERIERQSAFVDAITMGMNKVIVGQKHLIESLLI
                     GLLADGHILLEGVPGLAKTLAIKTLADLIKADFSRIQFTPDLLPADVIGTMIFSQKQE
                     AFNIRKGPIFANFVLADEINRAPAKVQSALLEAMQERQVTIGDKTFKLEEPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVINYPKKEEEKLIIRQNIAKEQPRVAPVLSPQDIV
                     AARDVVRQVYIDEKIERYIIDIVFATRFPEEYGLDSMKDMISFGASPRASINLALAAR
                     AYAFIKRRGYVIPEDVRAVCYDVLRHRIGLSYEAEANNLTSEEIITDILNAVEVP"
     gene            complement(15811..17514)
                     /locus_tag="PKH68_02740"
     CDS             complement(15811..17514)
                     /locus_tag="PKH68_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX11885.1"
                     /translation="MSKEKISMQEIVDLMVVHRNMTRKSAEEFVRQLLSTIEDALLEG
                     DSVKISGFGTFKPQWNEPRKSIDVNTGNEIIIEGFYKVAFVPDNELKELINEPYSHLE
                     AIEIGNKAEDIIESEPENDNFKQVIEPMRVFEEQAEEIKNLLNEIGALSTNKMTAKKE
                     KSSVEKAIEPAENLIETPVEVMDDVENIVLADDELDNRVIAEEEELSQPELTEKPEVI
                     AEETMPIIIDREEEPVSDIEDAKVIGFSETAKQISIEAKREQDTRPDDFDIIREGGKI
                     IFPGKKSTVDNTHPEPELVADIKPQTEQANAKNLMQDEIVAEETLPADLIIENSPEIS
                     QSVNHEPPYFAPESPVNDEGEVLKQDVTPKSVVETVSDTEKAATDAVSVPYVPESVVE
                     ELPDEVDIEEIKKRPVWIYILSVLALLALAAVAAWFYYPGIFNNLNNKTAQTLPKVAE
                     QTNLNPVEPVIPDTLAAPNALPDTIINVFDAPRQFTEYIATEPMKRGSMLSIFAKKHL
                     GHPYFWVYIYEANKDIIQDPNNVPLGVDIKIPKVDPRLIDVNNPECVQFALKLSEQYL
                     K"
     gene            complement(17516..17797)
                     /locus_tag="PKH68_02745"
     CDS             complement(17516..17797)
                     /locus_tag="PKH68_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX11886.1"
                     /translation="MNNKELISNLALKMNLPKSEINDMLEAFSHTCIDRLKEDNSIGF
                     QGFGTLEVRKKEERISVHPVTKIRTLIPPKLVVSFKQSNILKEKLNSQQ"
     gene            complement(17993..19282)
                     /gene="rimO"
                     /locus_tag="PKH68_02750"
     CDS             complement(17993..19282)
                     /gene="rimO"
                     /locus_tag="PKH68_02750"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNX11887.1"
                     /translation="MKKNTVDIITLGCSKNLVDSEQLMRQFEVLGYNVRHDATNPDGE
                     VVIVNTCGFIGDAKEESINTILQLAEQKKEKNNKLFVMGCLSERYLKELQLEIPEVDK
                     FYGKFNYTDILKELGQEYRADLRLERSLTTPSHFAYIKISEGCNRMCSYCAIPIITGR
                     HRSRSMEDIEDEVRILVDKGVKEFQIIAQDLSYYGIDRYKTSKLAELIRRLSDIKGVE
                     WIRLHYAYPAHFPYDLLTVMRERDNVCNYLDIALQHISDNMLKKMQRNFTSAQTYELI
                     DRLRAEVPCIHLRTTMMVGHPGETEEDFEELKKFVKWARFERLGAFTYSHEEGTYAYK
                     NYTDHIPEEVKQRRLDALMEIQREIAAEINETKIGKTLKVIIDRREEEYYIGRTEFDS
                     PEVDPEVLIPIAVEGVKIGEFYQAKIVDASDYELFAE"
BASE COUNT         5027 a         4150 c         4038 g         6087 t
ORIGIN      
        1 ggcgaaacat tcatcggttt actcaaagcg ctggataata ttgatgctga tgttcgctac
       61 aggatttcat ccgtggaacc gaacctgctc accgatgaga ttattcaatt tgttgccgac
      121 agtcgccaca tcatgcctca tttccatatt ccgctgcaga gcggcaccaa cgaaatactt
      181 caactgatga aacgccgcta taccgtagaa ttgtttgccg aaaagatggc aaccatcaaa
      241 cgtatacttc ctgatgcttt tatcggggtg gatgtcattg tgggagtcag gggcgaaacg
      301 gatgaactgt ttgagcaatc gtttgaattt atccaaagtc tggatttttc gcagcttcat
      361 gtatttacct attctgagcg cgcaaacacc aaaatgcttg aaatagaaca caatgtgcct
      421 gtaaaagagc gtaaacgccg cagtgaacgg cttcatgagt tgtccgaaga aaaaacggca
      481 gctttctatg cttctcaaac cggaaaaaca gcaagcgtac tctgggaagc caaaaagaac
      541 gaagaaatga tgcatggatt taccgaaaat tatgtacggg tagaaaggcc ctatgataaa
      601 aaacgggtga acacggcaga aactgtgact ttgggtgatt ggaacgaaga taaaactgcc
      661 ttgaaagcca tttaacggcc aatagaccga gaaataaatt tgattttatt ataacaaaaa
      721 atattcccaa tacgtaatac cggttaccat catgttagct aaaagaatta ttccctgcct
      781 ggacatcaaa gacggaaaaa ccgtgaaagg tatcaacttt ctgaatatca ccgatgtggg
      841 tgatccggtg gaactgggca aacgttacgc cgaaatgggt gccgacgagc tggtatttct
      901 ggatatcaca gccaccaacg aaaaacgaaa aacgctttcg gaactcgtta cacgcattgc
      961 gaagcagata aatattccgt tcacggtagg cggaggcatt tcatcggtgg aagatgtgtc
     1021 ggtgctgctt tcgtgcggag ccgataaaat ttcggtaaac acagcagccg tgcgcaatcc
     1081 gcaactgatt aaagacctgg ctgataattt cggcagccag tgtgtggtgc ttgcagtcga
     1141 taccaaatgt atagaaagtg aatggtttgt ttttctgaac ggcggccgtg ttcccactga
     1201 aattaaaacc ttagactggg tgaaacaggc tgtagcgctg ggagccggtg aaattctgct
     1261 tacctccatg aaccacgacg gaaccaagga cggttttgca ctggatatta cccgggcagt
     1321 atctgaagct gtgaatgtgc ccgtaatagc gagcggagga gccggaacta tggagcattt
     1381 tgtagatgtt tttgatgccg ggaaagccga tgctgccctt gctgccagta tttttcacta
     1441 tcaggaaata ggtattcagg aactaaaaca ttttctgaaa gaaaaaggcg tagaagtgag
     1501 actatagaaa acctccgggg aaaccaaaac gatggcttcc ccgctttttt tatttcttgt
     1561 cctcacccga cttcatagtt ctattgcagc ctgaacgata ccggaagata gtatttcacc
     1621 ttcaccgcat taccgccctg cctgcccggt atccatcgtg gcatggattt aattacccgc
     1681 accgcttctt tgtcgaggct gccatccaca ctgcgctcca cccgcacatc ctgaatagaa
     1741 ccatctttac ccactacgaa tgaacaggtt acctttccct ggatattcat ttcctgagca
     1801 atggtcgggt aactgatatt attgttcagg tattccatca atgctgcatc gccgcccgga
     1861 tacgaaggtg gttgttctac cactatgtga acttcctcat caggagcctt ttcgcccacc
     1921 accagcttat ccttttccag gtcagcgata tttttcccaa actgttcatc tgtgcctatg
     1981 acagttgttg ttgagatttc gactttagcc ccaacaagtt catcctgcga tgttatctga
     2041 ttgttttcat ttaccttgtt gtcatccatg ataaccggcg ctgtaaactt gatggtactt
     2101 ttcaactcta caggcggagg tgcttcgtat cgcttctcga tattttcttt gggcacttct
     2161 gcgtttttca tactgatatc cgacatttgt accacatctg ttattttttc cttttgcttg
     2221 ttctgattat aggtattaat cagacttgcc gcaagaaatg taagtacagc tgctgcagcc
     2281 actacgatga atgctacggc atgtcttttt ggggaatcag agcgaagcgt gtatgctccg
     2341 tagtctttgt tctttccttc aaaaatgatg tctaaccatt cttgtgaagt taaattaatg
     2401 tcgtttgtca tgatgattaa tttttaaatt gtttagtaac ccgattttac ctgagcaggc
     2461 agttcatttt ttccagtcgt aagatgcagt agtttattct gcaatttttt tgaaactaaa
     2521 actgctttgg attgctcatg acaaagttaa agcccgtaca agggttttgt acgggcctat
     2581 gctgaagtta acgccaactg ttgggttatc ttactatttt acagtattat acaaattgtt
     2641 tattaacgac tgaaggggat tacaatgggc taaaatgtgc tggaaaatca accttctgtc
     2701 tccatttttt cgggaatttt caccttattc tccagcattt tcttcttcag tttcgattta
     2761 cgcacgcgga tactttccac agatgtattc atcagcgtag ccatctgctg attatccgat
     2821 cctgtagcca taaatgccat catcattctt tcactttcat tgaaaagacg gctttcttcg
     2881 gaactaattc cggtaagacc cgagaaaagt tcgtcggtaa ggaactgggg ttcggaaatg
     2941 ttggtggtct gcacgaaatc gtggtactcc gtttcaattt tttgcaggat ttgtggattt
     3001 ttggcgatgt ggctgttgct ttgcaggtcc agcagaaaat tctttatggc attattgcgc
     3061 tgtgatatct gccggtatat gggtattacg aattgtttct ccacgagttt tctctctgcc
     3121 agctgtttat cgagcatcat ttgtttttca ttggcttcag ctatcagctg tttctgttcc
     3181 agtatctttt tttcctgctg cagcgatttt tcctgttctt tctgaagccg cgaccggttt
     3241 ctgaaaatca gcagggtaat aatcaatccc attgtgagga taatgagaac caactgaagg
     3301 attatattgt tgtttttaag tttcattgcc ctgttctcgg ccgaagcaag atcgtatttg
     3361 cgttcgagtt ccagtatctg cgtattgagc tgattgttga tggttttatt catcaggcgg
     3421 taaacctgct gataggcagc agtgctttta tcccagtttt tcatctgcga agccagttcg
     3481 gccacattca ggtaataata gtaattcaga taaaatgtag tatcgcggat attccgggtt
     3541 gccagctcgc cataatgcag tgcgctgtcg ggttggttca gatatttgta gttatccgaa
     3601 atgcgataat aatcggcaag cagggcattg ctgtccttgt tttgtatatc ggtacgaagc
     3661 aggttataat ctatcttgag tgctgtgcgg tatcgtttat tgctattgta gtacaaagct
     3721 tcggcatttc ggatgtcccg tatttgttct ttatttgcat ttgaaaaaga ctgcagagtt
     3781 tcgagcaggg tgcgggctgt gagaaaatcg agtgctttca tccggttcca gtacatttcg
     3841 cggcaggttt tgaacaaaat tacactgtct ttcaaatttt cggcaatcac gtaggcttgc
     3901 ctgaagtact catgagcctg acttacgtta ttatttttgg tatgaatgac gcccaggtag
     3961 tagcaggctt tctgcctagt tttcgggtca agccgtgata actctgagag ggtcaacgct
     4021 tccttaatgg gcaaataagc gcgattgtca gagattccgg tgtgaaaccg cgtaattccc
     4081 tggtatatca atgctctcac ataattgttg ggagtataat ccgacctttg ggcaaacaca
     4141 tcgactgtag cacttatcag actatcggaa ggcaatgttc gacccatatt gtcggctgcg
     4201 atagaaagta tcaagcctgc ataagcccga tcgtaactgg taagttgatt ttgtttgatc
     4261 agattattga caacattata aatatacttt ggataccggt aggccatgct gtccagctca
     4321 acaagatttt cagccggttg ctgttcatct gccggtcgac aggctgccag acttatcagt
     4381 agtaaaataa gtcctgcata aaatccggat tttaataatc tgtcagtttt cggcatctca
     4441 ggtggattgt tttttgaaaa aaatcgtatt acataaccaa ctctgcgaat aattcttcca
     4501 gtttattttc gggattatct gtaaatccat tgtgcgaccg ggaagtctgg atacaggtac
     4561 tcctctcggc cgtcagccag cgaaaacgtt ccgctatttc caactctgcg atttttccgc
     4621 cctgtttttc accattacaa atgatggcaa aggatttcag cgtgtcaccg agcagtccaa
     4681 aatcgagttc ggttgcagtc cggcaagtct gttcaacatc ggaacggtat ttcatgccaa
     4741 tatagttggc ttcttttgaa aacaaaataa tgccgatatt gataaactct tcgcgttcca
     4801 ccttcgggaa aaggcgtatt acagcgtatt catacaagta ttttccgcgc atggatagct
     4861 tcatttaaaa agatttctga ttgtgccagt ctgtctgtca ggaacttaaa atatgcgtcc
     4921 cggatcattt caggcgtttc gccgtcgtgg ttccagtgtg cccattcatc aggaataaga
     4981 ttaaccacat cacggagcat ttcatcatta agaatgttgc ggcattcttc atcggcttcc
     5041 cggagttgtg acgcaaattc gagcaataca tggtctttta tcaacggaaa aggattggca
     5101 gcctgttttt cgcggtttgc ccagttgtgg tgaaagtaga gcgtggaacc atgatctatc
     5161 agccagagtt ccttatgcca catcagcata ttggtgtttt tcacagtgcg gtctacattg
     5221 gtcagaaagg catccatcca cacaatcttt gaagccaacg cagcatccac tttggttgta
     5281 accggatcat aagtcagcgc tcccgaaaga aaatgcagtc cgagattaag accagtgctt
     5341 gctttgagca ggtcctgaat ttcctcatca ccttcggagc gtccgaatgc ttcgtcaagc
     5401 tgaacataaa ccagttcggg aattcttaac ccgagaattt tcgccacggc gccaccaata
     5461 agctcagaga tgagcatttt ggtgccatgc cccgatccgc taaacttaac cacatacctg
     5521 aaaccatcgt cggcttcggc cagtgcaggc agggagccgc cctcgcgcaa cggtgtgata
     5581 tagcggttta catttactgt ccgtaaattc atagcttgat gtttattgat taaaaccggt
     5641 atctgatgcc ccacaaatgt ctgaaactca aatcaccggc atcatctata ttgacatcca
     5701 tagccggggc aaactccacg agcagtgaca attttttaaa gctttgcaat ggaaaaactt
     5761 caagtaccaa tggcaccgta atttcattga ggtaaatttc attgaaaggc tgactgttca
     5821 aacccagacc tacggaaaac ctatggtact catgcgtttt gaagttgtgg aacgcatcca
     5881 gttcgaaacc caggttatgg aaattggaat ttgcaaacgt ttttagttcc acactgaaag
     5941 gtttttgagt gtcggtactc acgccgagcg cgtaaatgga ataggaagtg accgaaagtt
     6001 gagctttagc taaagttgaa gctgtaacca gaaaagtaat aatcagtaca agtacgggaa
     6061 acgatttttt catgtgttta attttatata tctgccccct aaccccctaa agggggaata
     6121 ttgagcgttt atcatttgtt gtgagttttt taatcatgcc cgtgtatgtt aaatataatt
     6181 aacctgggca tgtgtgtttc attttattct tcgttgaatg agttagcagt aaagtttcga
     6241 acaaagttaa aataaaaaat cagaaaaagc gaggagttgg atttttattt aatatatcca
     6301 cagactgtat gggatcagtg ctacgaatga agcacctaaa acccataagg gcaaagacat
     6361 attgatttca gctgactttt tgaaaacaat tttgtcggga aggacttttg taaacaacca
     6421 atacaccagc attgccgctc ccacacctac gattgcaccg ccaataacat cgccggggta
     6481 atgaacaccc aggtaaatgc gcgaataagc cactatggtt gcccaaagaa atatgataat
     6541 agtatatttt cggcttctga aaaacaggga agttagcatg gccagtccga aggagttggc
     6601 agcatgagag gaaacaaaac catattttcc accgcgataa cctttaagca gacaaaccat
     6661 gttttcaaga tcaggattgc gtgaaggacg aagccggtgt accatttctt taatcaaacc
     6721 ggacgaaacc tgatcagcaa tgataataca gacaatcaaa gcaagaataa ccagccagga
     6781 ttgttttttc aatgatttaa taaggataat aatcagtgca acataaaaag gaatccaggt
     6841 aagtttatcg ctgataccgt acatcagccc atcccagaaa gcaggatagt cgttgttcag
     6901 tgccaacaac cactgtttgt caagcgtgtc gagtgtttga aggatgtcca ttttgttggc
     6961 gattggttat tggttattaa gttattggtt attaagttat tggttattaa gttattggta
     7021 attggcaatt ggcaatttga atgtttcaac aagcctgtag catttttcta ctcccccttg
     7081 agggggcagg ggggctaaat cctttctatc gcggtttctt ccacccagcc tacagcgcca
     7141 ttctccagtg taatttcagt ccagttgccc agtacgcttt ttacttttac caatgttcct
     7201 tcatgaagct ggaaaaggtc tgttccgctc tggtccggtg aacttttgac ggcaactgct
     7261 cccatcataa tgaccgcttc gtggtgattt ttgatctgat ctttgcgaat gcctgaaaaa
     7321 cttaacgcta cgacacacaa aataccgaac aggacggatg caaaaaaaga atattttctc
     7381 cgggttctta ttcgggaaaa tgcaaacagg aagaatagtg ccagcattag aataaaacag
     7441 acaacactaa tcaccgccca ctggttggat gtgtaggact tgataagtga gttggtccat
     7501 tttcttacaa aaaaagtagg ggaagtattt acattgtcca caatcttgct ttcggcaatt
     7561 tggaggttgt aagatgcatc atcgaagccg ggatcaatac gcagggcacg ttcgtaattc
     7621 aatatggcgt gacctatttc gttggcttta taatagctgt tggcaagatt ataatacaat
     7681 tcggctgaaa cacctttttt cagttcttct tcataaattc gggcagcatc ggtgaattgg
     7741 ccctgcttat acatttgatt agccaaagcc actttatcag cagatttttt ttcctgagcc
     7801 actgtctgac cgaacatcag aaaagtaagg cagaatatga tataaatccg tttcatatta
     7861 ttttttctta aaatattctt ccactttacc aatagcctca atggtttctt cgtagagatt
     7921 acccatttcc tgctggcccg aattgggagc ataactggca tattcacagg tattcagaat
     7981 gttggtaaat tgattagtca gttcattgtc aataccgcgt tcagtcattt ctgcagcgat
     8041 attttctttg ttaagcgctg caaccggaat ggatagttta tcggacagat aggtccaaac
     8101 cgccttcatc acctcttcgt agaattggga tttttttcct tcagtaagga atttctgtgc
     8161 cagtttcaac cgtttctgag ccactttatt ggcttttttt gttttcatca ggctgatatc
     8221 ggcattttct ctgatttgct tacggaaata tataaacaga attccggcaa cgagcaatgg
     8281 aattaagaac atcatccaga acagcatgga gccgaacagt ggcgtttctt ctgcatgaag
     8341 tttgattttt ccggtatgaa tataccggat atctttggcc atctgggtga cgtcttcctt
     8401 gttggtgtaa ttacctacaa tcgtattttc accaccgaca cctttggcta cattgagcct
     8461 gtaagcggga gttcgcagtg ttttgtacgt acggtcattc agatcaaaat aggataattc
     8521 ggccgaaggg atattaaaga cacccgcatg ctggggtata aacatgtatt caatggtttt
     8581 tgttcccgat acaccgttgg cattggtact aaatttatta tctgctttgg gatcatagac
     8641 ttcaaatcct tcgggaaatt ttatttcggg gttttgaagc agtttcatat ttcctgtacc
     8701 tgaaatcacc actttaatga ttccggcatc gttggctttc aggttggttt tgctgataga
     8761 tgagctgagg ctgaaattgc ccactgcacc ggtaaacgaa gccggtttac cagcaggcag
     8821 ggcattgacc gtaatgcggg ctcctggtgc tgtcagactt ttctgtacat tgctgtatga
     8881 atcgaagaaa tcgtcaaaaa tactgcgaac ctgtgccttg ttctgaattc ttaaaatcgc
     8941 cgtaaagttt gctttatcga tgttaatttc gcccgttcgt tgtggaaaca gcaacacctg
     9001 atataagacc accgttccga aatttttacc gttatagttt tcgtatgata gctgagcatt
     9061 tttgggctgc gctatttcct gtttgagaaa tccgttgaaa tccggaaatt tcatttcggt
     9121 aaactgagcc acgtcgagca aagtgtacag cttataggtc accagaatag cttcctgttc
     9181 gtacacattg gttttcgata cactggtacg aataaatatg ttatcgttcg atattcgttg
     9241 tgtttccgct ccggtggcag gagaacctgc cccaccctgt ccctgcgatt gagttttgtc
     9301 agccggcaat accttgatgg tcattgcatt agacacatac ttttcatcat ccacctctat
     9361 agctgcaccc ggaattttga atgtgccgac tttattggga agcaacgtaa aggtatagga
     9421 tacattgacc gaagaagtag ccttaccgtt tatattctga taactcgaac tgtgcgattc
     9481 gaatggtccg gcaagaattt cgaaaaactg aaaatcgggt gcgcgcaagt ctttggcgga
     9541 agcattgacc gaatacacta actgaaaggg tgtatccata taaactgtag caggtgccga
     9601 ggctgtaaaa ctgaccttgt cctgtgccga tagagcggta agggtgagaa gtacagatgt
     9661 aaaaaatatc agtattcgtt tcataggata tgtgtctgct tattgctcag ttgtaattct
     9721 tttaatggaa atatataaat ctactgttca ataaattacc agtctttttc tgcttttttc
     9781 cggccctgta tctgggcttt tttggctttg tcctgagtat ctttttcgtc ctgttgcaat
     9841 gcttctaata tctgttgagc attttctttg gacatctgag gctgctgagg ttgtggttgt
     9901 tcctgttttt tatctttatc gggctcattt ttgggttgct tctgctgctg gtcgttgccc
     9961 ttgtcctgct tatctttgtt atccttgttc tcctgttgtt gtttcttgag cagcatctga
    10021 gcgtaagcaa gattataacg tgtgtcgttg tcttccggtt tatttttcaa tgcagtttta
    10081 tatgcatcaa tagcctcctg tattttattt tcacctatca atgtattgcc gatattgtga
    10141 aattccgaag cggctttttg tttgttatta ccggcaaagg ggattgccga tttatattgc
    10201 tggagtgctt cactgtactt gccctgctta aaaagtgcat ttcccaggtt gtagttggct
    10261 tcaaacgatt tgttgttttt taccagtgcc ttacggtact ctatttcagc ttcggtatat
    10321 tttttattgt tgtaatggcg attacctgtg cgcacatcgt tgctttcctt ctgcggataa
    10381 cccaccaatg gcaagagcat tgctacaatc aacagtatat ttttcagttc tttcatcttt
    10441 ttaaaaaaat aaaaaatcaa cttatgcttt aattttctga accttcacat caaatagacg
    10501 aatgcggctc agccatttat tcttccggga gaatacaaag aattctatca acataagaaa
    10561 tagtgcaatg aatgcaaatg actgaaactg ttcgttgtat tccgaaaaca ccctggcatc
    10621 catttcgttt ttagcaagtg catccagctg gcgacttacc gtacggagag ctgcattact
    10681 gttgtcggag cgcacataca cacctccacc ggcctgtgct attccggaac acatctgttc
    10741 gttcagcttc gaaacgacca catttccatc cttatctttt ttgaaactta ctgtttcacc
    10801 cacaggaatt ggcgcaccct cgggagtacc cattcccacc acaaagattt ttattccgtt
    10861 gtcgttggcg agtttagccg cagctatagc atcatcctcg tggttttcac cgtcggtaag
    10921 tacaatgatg gcgcggctga cacctgattt ctggccaaat gatttgatgg caatgtcaat
    10981 agccgatccg atggcagtgc cctgacgggg taccatttgt gggttgatgg atgacagaaa
    11041 cattttggcc gatccgtaat cggatgtgat aggcatctgc acataggcat cgcccgcaaa
    11101 cacgatcatc cccaccttat cttcctccat tttatctatc agttgcgata gtatctgctt
    11161 tgctttttga agccggttgg gctgaatatc ctgcgccagc atggaattgg acacgtccag
    11221 ggcgatcatc acttctatcc cttttttgcg tactttttct tccttggtgc caaactgagg
    11281 ttgtgcaagt actacaataa tgagaattag agcaacaagt tgcatataaa atttccatct
    11341 gggacgtgca taggacacat tgggcataag cgttgccaat aaggcagcat ttccccacaa
    11401 tttgatattc tttttccgaa gtacagcagc gcgcacaaaa gcagctaccg gaattactgc
    11461 cggtaaaagt aaaaaaagat attcgggatg tgcaaatcta aacattatat gatgttataa
    11521 atctttcttt atgagctgtt taattctaat ttaattacct ataaatctat ccggatttag
    11581 ggtaatgttt tcatccaggt attacgaatg agtatttcgg caaaaaatag caggaatgct
    11641 aaaagtgcaa aaatgaaata ggtttcactt ttgttactga attcctttac gctgattttt
    11701 gttttttcca actggtcaat ttcctgataa atagccctga gcttgttgtt gttggttgcc
    11761 ctgaagtatt tgcctccggt aatatcagca atgtctttca gtgttttttc atcaaactgg
    11821 gtttcttcct gctgatagcg cactccgaat ggagtttgta tcgggatatt gatgaccccg
    11881 taagagccca ctccgatggc gtacacgcgg ataccgaatg ttttggctat ttcagcagcc
    11941 gtgatgggcg caatatcgcc gcggttgttg gaaccatcgg taagcaggat gactactttc
    12001 gattttgctt tgctgtccct gatgcggctc acggcattgg ccaaaccaag cccgatggca
    12061 gtcccatctt caatcaatcc ataccggatg cccgaaaaca gattgatcag cacaccgtga
    12121 tcgatggtga gcggacactg ggtgaaactt tctccggcaa ataccaccag gccgatgtta
    12181 tcgttcggtc gggacagaat aaattccgat gctacggttt tggcagcttc gaggcggctt
    12241 ggccgcaaat cttcggcctg catggtacct gaaatatcca gtgccatcat aatatctatg
    12301 ccttcggtgt tttcggtacg ccagttgttg gacgactgcg gacgtgcaat agccagtata
    12361 agcatgctaa tggccagcaa acgcagaatg aagggtaaat gtctgaactt catgcgtctg
    12421 gtccgtggca aatcctttaa cgtctgcacg gatgaaatct gcaggctggc ttcagacctg
    12481 tgtatctccc tgatgtacca aataatcatt ggcagtagta aaaggagcag aaacaaatat
    12541 tcgggattaa gaaatgtcat cttctatttc gtcaactgtt gttattaaca ggcatttgtt
    12601 ttatcttgtt tcgggttcgg cagttttgtc ggctttgctt tcttctgact ttatttcttc
    12661 tactgtagtc tgatttacaa acagatatga attcatcaga ctcagttcat tttctacggt
    12721 ttcaggattc catttggcaa attttaccag atcagccagt cggaggattt gtttcaatcc
    12781 ttcgaaagca gcaggtttat ccactttcag gaattgtgaa ctacgcagta tttcatcgga
    12841 ggtcatttcc atgctcggga tattaaacac cctttcgata taggtacgca gcacgtctgt
    12901 cagttcggta tgatattctt ttaccctgcc tttttgccag attttctctt ccttaatttt
    12961 atcaagccgc cgcagggctt ctatgttggc aggaataatc tcttcaggcg tttgtgtatg
    13021 gaatacaggt tttttcatta caaatttgcg caccaaaaca aacaatatca ccgccaaagc
    13081 caacaagagt attgccagca gcaatttttg taaaaagttt ttccagtcaa agggtggttt
    13141 atacaccggt ttaatatccg tcaggctatt ggcggctgta tctatcacga atggttgaac
    13201 cactttgaga gaagtagaat tggaccaggc agtatctttt ccggtaacaa aagggaaagc
    13261 cggcacataa accagcgaat cctgaaaaga agttagtttg taacggatat ttacctgaat
    13321 tttatcgtta gagagcttca ccgtatccat tttggcttgc tccacgatgt cgagattgcc
    13381 aacaagggta tccgagaaaa tcggagccgt tactttctgg ccgggtgcct gaagaatttc
    13441 gaaggtaaat ccggtctgat tgccaatcca aagctgagtc gaatccattt tcgctttgat
    13501 gataaccggc tgtgccagca tcactgaggc acaaaaaacc attatagtca agatgaaata
    13561 atgaatctgt tttctcatat ttattttgga taatttaacc gtgttattaa gctcttaatc
    13621 tgaaaagttt catcaatgcc cgcacataat cttcattggt gctcatagta acagaatcta
    13681 cccccgattg tttgaatgtt ttctgtaatt ccagttggcg ttcgttccac ttgtgtttgt
    13741 atgaagttcg taagcgttta tcggcggtat ctatccatat tcgttcaccg gtttctgcat
    13801 ctttcagttt gataagtcct acattgggga tttcagcttc gcggatatca tacacctgca
    13861 aggcagctat atcgtgcttg cggttggcta tctgcagagt gcgatcaaat ccgtgatcca
    13921 tgaagtcgga aataacaaaa gccgtagagt gttttttgat ggcgttggtg aaatacttca
    13981 gtgcaccccc aatatcggtc tgccggctcg atggttcgaa taccagtaac tcccgaataa
    14041 tgtgcagcac gtgctttttc ccttttttgg gaggaataaa tttctctatc ttgtcggaga
    14101 aaaatatcac gccgacttta tcgttgtttt gaatagtgga aaatgcaagt gtggcagcca
    14161 cttcggtgat aatatccttt ttcagcataa atccggtacc aaaatcgcga ctacccgaca
    14221 catccaccag caacattacc gtcaattcac gttcttcttc aaataccttg atgaatgggt
    14281 gaccaaaacg ggcagtaacg ttccagtcga tggaacgcac atcgtcgcca tattgatatt
    14341 cacgcacttc ggcaaaggtc ataccacggc ccttgaaggc tgtatgatac tgacccgcaa
    14401 aaatattttg cgagagtccg cgcgttttta tctctatttt ccgaactttt tttagtaatt
    14461 cgtgggtttc catttacaca gttattttgc tgccgggttt gttgggttac aacttatcat
    14521 agccatcagc aggtttttta gaggctgtat tattctgatt ttcaaacagt caaatgacat
    14581 tcgttaataa atcagggaac ctctactgca ttcagaatat cggtaatgat ttcttccgag
    14641 gtcaggttgt tggcttcagc ttcataactc agcccgatac gatgcctcaa tacatcgtag
    14701 catacagcgc gaacatcttc gggaataacg tatccgcggc gtttgataaa agcgtaagca
    14761 cgggctgcca gagccagatt gatggaagca cggggtgatg cgccaaagga aatcatatct
    14821 ttcatcgaat ccagtccgta ttcttcgggg aaacgggtgg caaatacaat gtcgataata
    14881 taacgttcaa ttttttcgtc gatgtaaacc tgacgaacca catcgcgggc tgctacaata
    14941 tcctgaggtg ataatacggg agcaactcgg ggctgctctt tggcaatatt ctgacgaatt
    15001 attaattttt cttcttcttt tttggggtaa ttgatcacca ctttcaacat aaaacgatcg
    15061 acctgtgctt cgggaagcgg ataagtgcct tcctgctcga tagggttttg agtagccatt
    15121 acaagaaatg gttcttcgag tttgaaagtc ttgtcgccaa tagtaacctg acgttcctgc
    15181 atggcttcga gcaaggcact ctgaactttg gccggtgcac ggtttatttc atctgccagc
    15241 acgaaattgg caaaaattgg ccctttccgg atattaaacg cttcctgctt ctgactgaaa
    15301 atcattgtac caataacatc ggcaggcaac aaatcgggag taaactgtat acgactgaaa
    15361 tccgccttaa ttaaatcggc caatgttttg atagctaatg ttttggctaa gccgggtaca
    15421 ccttcgagca ggatatgacc atcggcaagc aatccaatca acaacgattc aatcaggtgt
    15481 ttctgaccta cgatgacctt gttcataccc atcgtaatgg catctacaaa cgcactctgg
    15541 cgttcaattc gttcgttaag ttccttaata tcaaccattt gatccatatc taataaaaat
    15601 tatatttttt ttcgtgagca aagatacaat ttattaaaat gttaaatatc tgatttaata
    15661 tctattaacc tgaatttaga gaatgtaaac aatcatactg gctgtgagtt acttcagttg
    15721 cctatttcac tctttttttg aatgtttatt tgtatttttt acaccaaaaa aggtctgttt
    15781 gacagacctt tttttagata aacaaattta tcatttcaga tattgctccg ataactttag
    15841 cgcaaactgt acacactccg gattgttcac atcaatcagt cgtggatcaa ctttaggaat
    15901 tttgatatcc actcccagtg gcacattgtt gggatcctga atgatgtctt tgttggcttc
    15961 gtaaatatat acccaaaaat acggatgtcc caggtgtttt tttgcaaaaa tgctcagcat
    16021 actgcctctt ttcattggct ctgtagctat atattcggtg aattggcgcg gagcatcaaa
    16081 aacattgatt attgtatcgg ggagtgcatt aggagcagcc agcgtatccg ggatcacagg
    16141 ctcaaccgga tttaagttcg tctgttcagc tactttgggc aaagtttgag cagttttatt
    16201 attgaggtta ttaaaaatcc ccggataata aaaccaggct gcaacagcgg ctaatgccaa
    16261 taatgcgagc actgagagta tgtaaatcca gaccggacgt ttctttattt cttcaatatc
    16321 aacctcatca ggtaattctt ccactactga ttcaggcaca tagggcactg acacagcatc
    16381 tgttgcagct ttttctgtat cggaaacagt ttcaaccacc gattttggtg ttacatcctg
    16441 tttgagtact tcaccttcat cattcaccgg actttcggga gcaaaataag ggggttcgtg
    16501 atttactgat tgtgatattt cgggtgaatt ttcaatgatt aaatcagccg gcagagtttc
    16561 ttcggcaaca atttcatcct gcatcaggtt ttttgcgttc gcttgttcgg tctgtggttt
    16621 tatgtctgct accaattctg gttcaggatg tgtattgtcc acagtacttt tcttgccagg
    16681 aaaaattatt tttccacctt cacgaattat gtcaaaatca tcgggtctgg tgtcttgttc
    16741 tcttttggct tctatgctga tttgtttggc tgtttctgaa aatcctatca cttttgcatc
    16801 ttcaatatca gaaaccggtt cttcttccct gtcaatgatt attggcattg tttcttctgc
    16861 gatcacttca ggtttctctg tcaattcagg ttgactgagt tcctcttcct ctgctatcac
    16921 ccgattatcc aactcgtcat cggcaagtac aatattttct acatcatcca taacttccac
    16981 aggtgtttct ataagatttt cagcaggttc aatcgctttt tctacagaac ttttttcctt
    17041 ttttgcagtc attttattag tagataatgc tccgatttcg ttcagcaaat tttttatttc
    17101 ctcggcttgc tcttcgaata cccgcatggg ctcaatcacc tgtttgaaat tgtcgttttc
    17161 aggttcggat tcgatgatgt cttcggcttt atttccaatt tctatagctt cgagatgtga
    17221 ataaggctcg ttgataagtt ctttcagttc attatccggg acaaatgcca ctttataaaa
    17281 accttcaata attatttcat taccggtatt tacatcaatg cttttacgcg gctcgttcca
    17341 ttgaggtttg aaggtgccaa aacctgatat tttcacagaa tcgccttcca acagtgcatc
    17401 ttcaatggtc gaaagcagct gtctgacaaa ttcttcggcc gattttctgg tcatatttct
    17461 gtgcacaacc atcaaatcga ctatttcctg catcgaaatt ttttccttgc tcatttcact
    17521 gttgcgaatt aagtttttct ttcagaatat tgctttgttt gaagctcact accagttttg
    17581 gtggtatgag tgtacgaatt ttcgtaaccg gatgaacgga aatccgttct tcctttttgc
    17641 gaacttcgag ggtaccaaaa ccctgaaatc caatagaatt atcttccttc agccgatcaa
    17701 tacatgtatg actgaatgct tcgagcatat cgttgatctc cgactttggt agattcattt
    17761 tcaacgccaa atttgaaatt aactctttgt tattcatata taaagttaga agttagaagt
    17821 ttgaagtttg aagtttgaag tttgaagtca gcagatttta ttcatattgt caattaatat
    17881 tattctttgc tctttgctct ttattctttg ctctttcctc tttgctcttt gctctttgct
    17941 ctttgttctt tgttctttgc tctttgctct ttgctcatat caactgttcc gtctattctg
    18001 caaacaactc gtaatcagat gcatctacaa ttttggcctg ataaaactcc ccaattttta
    18061 cgccctccac agcaatcggg atcaatactt cgggatccac ttcgggcgaa tcgaattcgg
    18121 ttcggccaat gtagtattcc tcttcccttc ggtcgataat gactttcagg gttttaccga
    18181 ttttagtttc attgatttca gcagcaattt cgcgctgtat ttccatcagt gcatccagac
    18241 ggcgttgttt tacctcttcg ggaatgtggt cggtgtaatt tttataagcg taggttccct
    18301 cctcgtgcga ataggtaaat gctcccaacc gctcaaaacg cgcccacttc acaaatttct
    18361 tcaattcttc aaaatcctcc tccgtttcac ccggatgacc gaccatcata gtagtacgca
    18421 ggtgaataca gggaacttcc gcccgtaacc ggtcaataag ttcataggtc tgtgctgatg
    18481 tgaaattgcg ttgcattttt ttcagcatat tgtcgctgat atgttgtaag gcaatatcca
    18541 ggtaattgca cacattgtcc cgttcgcgca tcaccgtgag cagatcatac ggaaaatgag
    18601 ccggataggc atagtgcaag cgtatccatt ccacgccttt aatatccgat aatcgccgga
    18661 tcagttcagc aagctttgat gttttatacc ggtcaatgcc gtaataagac aaatcctgag
    18721 caatgatctg aaattctttg actcctttgt caaccaaaat tctcacttca tcttcaatat
    18781 cttccatgga gcgagatcgg tgccgtccgg taatgatagg tatggcgcaa taggaacaca
    18841 tgcggttgca gccttccgat atttttatgt aagcaaaatg tgaaggtgtt gtcagcgagc
    18901 gttcgagccg caggtcagcc cggtattcct gcccaagctc tttcagaatg tcggtgtaat
    18961 tgaattttcc gtagaacttg tccacttcgg gaatttccag ctgcaattct ttcagatatc
    19021 gttccgacaa acaacccatt acaaatagct tattgttctt ttcctttttt tgttctgcca
    19081 gttgcagtat tgtgttaata ctttcttcct ttgcatcacc aataaatcca caggtattga
    19141 caatgaccac ctcaccgtcg ggattggtag catcgtgccg cacgttgtaa ccaagcacct
    19201 caaactgacg cattaattgc tcactgtcaa ccaggttctt agagcaacca agcgtaatta
    19261 tatcaactgt attttttttc atacgcaacc ccctaaatcc cc
//
LOCUS       DAOPZK010000025        19153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_2992_length_19153_cov_21.168656, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000025 DAOPZK010000000
VERSION     DAOPZK010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19153
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2992_length_19153_cov_21.168656"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..790)
                     /locus_tag="PKH68_02755"
     CDS             complement(44..790)
                     /locus_tag="PKH68_02755"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF004079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNX11888.1"
                     /translation="MLLGLMGAMPEEMDKIIDALTDKKVVERGSRLYYRGKLYGQEVV
                     AVFSRWGKVAAATTATNLIVEFGVDRIVFTGVAGAISDDLNIGDVVIAQRLFQHDMDA
                     RPLMRRFEIPLTGKTSYEISEQNSGLMVEAVHNFLKNNNQLRNFAMEQHAPTPKLMLG
                     DIASGDLFISGTEMKKALIRNLPSVVCAEMEGAAVAQVCNDFDVPLTVVRIISDTADE
                     QAHTSAIGFVNQYGGEYSLAILREYIQLLK"
     gene            complement(864..2090)
                     /locus_tag="PKH68_02760"
     CDS             complement(864..2090)
                     /locus_tag="PKH68_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNX11889.1"
                     /translation="MVQFLFFLIIFILIADFILERSLSFLNAKAAAQPIPSLLSDVYT
                     HSQYRKQQLYFQANTKFGMITSAVSLTVVLLMFCTGGFGWLSQLVGGWSENPIVVSLI
                     FFGLIFLANELISIPFEWYQTFVIEERFGFNKMTPKLFITDKLKGYLLSVVVGGAILT
                     LVMWIYRLNPTYFWLIAWSCITVISLFISFFYSELIVPLFNKQTPLEAGELRDEIEKF
                     AVKSNFKLKNVFVIDGSKRSTKANAYFTGFGKKKRIVLYDTLIRDLTTDEIVAVLAHE
                     VGHNKHNHTLKNMMLNMPYTLLMFFLFGIMLRSQTLAQALGGSEASFHLNALGFGFIY
                     SPLAMLIGLGMNILSRRFEYQADAFVKVNGLSNQLISSLKKISSKALSNLTPHRAYVF
                     VHYSHPTLYQRIKALL"
     gene            2170..3414
                     /locus_tag="PKH68_02765"
     CDS             2170..3414
                     /locus_tag="PKH68_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX11890.1"
                     /translation="MKYYLCYKDNLTTIHLKMAMYLTSLIAFTALILIPDLWFFMKMK
                     KHHFYPWFRLLSLVPGFLFILSFFYIRFGLVYNSSYQVTAGISWILFAFSVIYTPKVL
                     YIVFYYINHSFNRIFNAKTRIFRIIGFALSVYMVTFLFYGAVVTRDDFYLKTQYIYVD
                     ELPRNFDGYKIAVFADLHLGNWNNRYKIMEPIITLLNEGKPDIIVFGGDLINNYENET
                     VGWEPYFRRLKARQGMYAVLGNHDYGDYTKWKTKEEKEENLDKTKRNIRNLGFRLLLN
                     EHVELVKGSDTITLAGVENYGSGHFENHGDLTAALKGTRPERKKILISHDPNQWDEEI
                     ATRKKDIFLTLSGHTHAGQIGITNSKIHLSPAAIIYPKWDGLYSQGNQYLYVNRGAGY
                     VGIPLRLGVPPEITLVVLKRKK"
     gene            complement(3441..4109)
                     /locus_tag="PKH68_02770"
     CDS             complement(3441..4109)
                     /locus_tag="PKH68_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNX11891.1"
                     /translation="MSVAQNIQEIRKNIPANVSLVCVSKFHPENAILEAYNCGERIFG
                     ESRVQELQAKQTKLPADIQWHFIGHLQSNKIKLILPTVSLIHGVDSFNLLKEINSRAL
                     KAGRVVSCLLQVHIAREETKFGFSMEEIRQMLSGNEFGSLENVQINGLMGMATLTDDK
                     EQIRAEFRTLRHLYDKLKREYFQNNENFLELSMGMSDDYQIAIDEGSTMVRIGSSIFG
                     MREY"
     gene            complement(4155..4586)
                     /locus_tag="PKH68_02775"
     CDS             complement(4155..4586)
                     /locus_tag="PKH68_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HNX11892.1"
                     /translation="MHNWFQCKVKYEKNAQDGGIVKTSEAYLVDALSFTEAEERINEE
                     MRPFISGEFLVSDIKREKVNELFANEAGDRWYRSKVNFISLDEEKGVEKRIATTMYAQ
                     ASSVKDALEVIEKGMKGTLADYEIAAITETNIMDVFPYKAE"
     gene            4794..5219
                     /gene="ruvX"
                     /locus_tag="PKH68_02780"
     CDS             4794..5219
                     /gene="ruvX"
                     /locus_tag="PKH68_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNX11893.1"
                     /translation="MGRVMAIDYGTKRTGLAVTDPMRIIANGLDTVPTHEVLDFLEKY
                     VAREPVDIFVLGLPTQMNGEESETMKQIKPFAHKLQKKFPTIELIYVDERFTSALAHQ
                     AMLTGGLKKKDRQNKALVDKISATIILQSYLENKINLLK"
     gene            5216..5761
                     /gene="def"
                     /locus_tag="PKH68_02785"
     CDS             5216..5761
                     /gene="def"
                     /locus_tag="PKH68_02785"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNX11894.1"
                     /translation="MILPIYTFGNSVLRKIAEPIDQDYPGLKDLIKNMYDTMVHAEGV
                     GLAAPQIGIPIRLIVIDLAPLKEDNPELGDFKVTMINPELLEESENEVEYDEGCLSIP
                     GINETVSRPEAIKISYMDENFIEHVEVYDGYKARVVQHEYDHLEGHLFTDKIKPLRRQ
                     LIKGKLTNIVKGKASCKYKIK"
     gene            complement(5831..6955)
                     /locus_tag="PKH68_02790"
     CDS             complement(5831..6955)
                     /locus_tag="PKH68_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNX11895.1"
                     /translation="MTEQQLNKKEYRYILIALILILGFILFRELQPYLSGFMGAITLF
                     VLLRGQMKYLTKKKKLNRGLSATIILLETLLVFLIPLTGITFLAIDTLSGIKIDPEII
                     KEQLLNFVNLIENRFNFKLFTPENLSFIPKLGTNVVQVLATSSYSLVINVVVILFVLY
                     FMLYSYEEFEITIREILPFTHDNKQSFVHETKSIIQANAVGIPLLAIIQGIFAYVGYM
                     IFGLDNALLYAVLTACATILPVVGTALIWLPLGVIYLFQANYVGGIGIILFGAIVIGG
                     VDNLARLILQKRLADIHPLITIFGVFIGIPMFGFWGVIFGPLLLSLFILFFNMYRRDY
                     IPGSVAKPIVTNHYIPELKIREKAEESEDKKEGNAVEQKK"
     gene            7445..>9035
                     /locus_tag="PKH68_02795"
     CDS             7445..>9035
                     /locus_tag="PKH68_02795"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNX11896.1"
                     /translation="MERKIYTQAEVENATRDYFKGDELAAKVWATKYALKDSFGNIYE
                     KTPDDMHRRLASEIARIEEKYPNPLSKEELFELLRDFKYIVPQGSPMTGIGNEYQVAS
                     LSNCFVIGFDGDSDSYGAIIKIDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNSALTST
                     GLVPFMERYSNSTREVAQDGRRGALMLSVAIKHPDAESFIDAKMESGKVTGANVSVKI
                     DDEFMNAAINNIPYTQQYPLYGNNPWVKKDIDAAALWKKIVHNAWQSAEPGVLFWDTI
                     LKESVPDCYADLGYRTVSTNPCGEIPLCPYDSCRLLAINMYSYVNKPFTPEAWFDFDL
                     FSKHVQLAQRIMDDIIDLEMEKIDRIIAKIESDPEDIDIKNTEFQLWEKIKTKTSQGR
                     RTGVGTTAEGDMLAAMGLTYGTDEAIKFSEKVHKAVALAAYRSSVTMAKERGAFSVYD
                     AKREENNPYISRIREADPALYEDMTKYGRRNIACLTIAPTGTTSIMTQTTSGIEPVFM
                     PVYTRRRKVNPNDKNVRIDFVD"
     assembly_gap    9036..9135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9136..10146
                     /locus_tag="PKH68_02800"
     CDS             <9136..10146
                     /locus_tag="PKH68_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNX11897.1"
                     /translation="RRRKVNPNDKNVRIDFVDENGDSWEEFIVFHHKFVTWMEANNYS
                     TTKNYTKEEVDELVAKSPYYKATANDVDWMKKVQMQGRIQKWVDHSISVTINLPNDVS
                     EELVGKLYEEAWRSGCKGATVYRDGSRAGVLVAMEEKKEEKKDDKERCFCYEDERLVR
                     PRELKCDVVRFQNAKDKWIAFVGLYDGRPYEIFTGLADDEEGIVLPKNVTEGKIIKVV
                     DEKGKRYDFQFTNKRGFKTTVEGLSHKFDKEFWNYAKLISGVLRYGMPIDQVIKLVSG
                     LQLDSESINTWKVGVERALKKYIPDGTQISEQACPHCGQKSLVYQEGCLTCKNCGYSR
                     CG"
     gene            complement(10203..11249)
                     /locus_tag="PKH68_02805"
     CDS             complement(10203..11249)
                     /locus_tag="PKH68_02805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX11898.1"
                     /translation="MNVSVVILNWNGEKYLNEFLPILLNNLDNVPDAEIVVADNGSTD
                     GSLQLLAEKFPTVKVDAFEKNYGFAGGYNKALKNINSEYYVLLNSDVEVTKDWLSALY
                     EFMTKNPDVAACQPKIRSYHRRNYFEYAGAAGGFIDKYGYPFCRGRIFGEVEEDLGQY
                     DDIKDVFWASGACMFIRSADYWDAGGLDEEFFAHMEEIDLCWRLKSRGKRIVCVPRSV
                     VYHIGGGTLNAEHPRKTFLNFRNNLIMLYKNLPQKNIKHVFDVRFYLDNLAALHLLLQ
                     GKYQNAKAVTDARTEFRIIQSKFSDQRKENILKSVNSLQKEISQHSVIYEFYIRRHKK
                     YSEISPNEETANMQ"
     gene            complement(11268..12134)
                     /locus_tag="PKH68_02810"
     CDS             complement(11268..12134)
                     /locus_tag="PKH68_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX11899.1"
                     /translation="MYHIINGLLKLKSLLPFSILYVLSDFTYLIVYYLIGYRKNVVRE
                     NLKRSFPGKTDRELLATEKKFYHHLCDLLFESMKMATISKEEMSRRMKILNYEPLLKH
                     YDENKSVLLITSHYGNWEWTSSFSMYLPADKPMYQVYKQQKNKLFDRLIYNLRIRFGS
                     VNVEVNELFDVLSSLKQQGKMGMIGLISDQSPSRKGIKYQSQFLNQRTPVITGTEIIA
                     KKYDFPVYFVQIRRVKRGYYTCNPVPICLHPQESGKFEITEKYIRLLEQEIMAEPAYW
                     LWSHRRWKFADK"
     gene            complement(12209..12541)
                     /locus_tag="PKH68_02815"
     CDS             complement(12209..12541)
                     /locus_tag="PKH68_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11900.1"
                     /translation="MKTQDDDLLVYDEDDAVKFILKLIPDEQKARINDDVINYVLDVV
                     YEYYEENGLIDEESTEEASIDEEEMMKFVLKASKKDKMNLTEDEIQLVLDGEYEYGKS
                     LGIYSEEE"
     gene            12740..13156
                     /gene="rnpB"
                     /locus_tag="PKH68_02820"
     ncRNA           12740..13156
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKH68_02820"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            13202..14524
                     /locus_tag="PKH68_02825"
     CDS             13202..14524
                     /locus_tag="PKH68_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNX11901.1"
                     /translation="MKKLIETIKYLYNFILYDIWRITGNELTRSRRFFYGVIKTIYLA
                     FRGYSRNKLSVRAAALTYSITFAIVPLIALMVTIAKGFGIESFIEKTLQDTFIAQANL
                     IPATMGFVEKYLETMQGGLFIGIGVAILLYSVMNLFMQIEATLNNIWQVQKSRSIFKQ
                     FTTYFAGVVLFPLLLTITSGLSIYINGILKNTFIYQIYSPFEQFTVALVPYLTGILMF
                     TIIYLIVPNTRVRFVNALIAGTVAGIAFQLFQTLYVNGQISLTRYNAVYGGFAAIPLL
                     LIWLYVSCLIFLVGAEISYVSQNLRNFDYEVDTDNISNRYKTYLEFFVAYIIVKRFEN
                     DKPPMTAEDIVNEYKLPIRLLNQILSILTNSKIIIAVKQDNNETAYQPALDINLLTMN
                     MMYSKIDLRGSDMFLNLKNDEMDAFWHKMQELQAKTDKIREKILLKDL"
     gene            14792..18097
                     /gene="secA"
                     /locus_tag="PKH68_02830"
     CDS             14792..18097
                     /gene="secA"
                     /locus_tag="PKH68_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNX11902.1"
                     /translation="MGFNDILKKIVGSKATRDMKEISPEVTKIKAVYEQIKNLSNDEL
                     RGRTELLKARIQEAIAPENERIEQLKATIEETELHLREKIYNDVDKLEKEIVEKIAVV
                     LDEILPEAFAIMKDTARRLNENSDLVVTATQFDRDLAAKYDFVSIEGDKAHFKNEWIA
                     GGNLIKWDMVHYDVQLFGGVVLHKGKIAEMGTGEGKTLVATLPVFLNGLTHRGVHVVT
                     VNDYLSKRDSEWMGPLYMFHGLSVDCIDKHQPNSEQRRQAYMADITFGTNNEFGFDYL
                     RDNMANSPMDLVQRKHVYAIVDEVDSVLIDDARTPLIISGPIPKGEEQLFEELCPKVE
                     KLVNAQRTLVTKYLADARTLMKSDKEKDREEGTLALFRSYKGMPKNKPLIKYLSEQGV
                     KAAMMKVEEIYMEQNNRRMPEATDPLYFVIDEKNNSIELTDKGIDLITGNSDDPYFFV
                     LPNVGDEIAELEKMPGLTPDEKQNKKDEILQNYSIKSERVHTINQLLKAYTLFERDTE
                     YVVLENKVKIVDEQTGRIMEGRRYSDGLHQAIEAKERVQVEAATQTFATITLQNYFRM
                     YQKLSGMTGTAETEAGELWNIYKLDVVVIPTNRPVVRNDMEDRVYKTKREKYTAVIEE
                     IVKLVEAGRPVLVGTTSVEISELLSRMLTSRHIKHNVLNAKLHQREAEIVAEAGQKGT
                     VTIATNMAGRGTDIKLTPEVKDAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSVF
                     YVSLEDDLMRLFGSERISSVMDRLGFEEGEVIEHSMISKSIERAQKKVEENNFGIRKR
                     LLEYDDVMNSQREVIYAKRRHALMGERIGVDISNMIYDGAETIVETAKNSEDFEGFEM
                     DLMKIYAMDSPIEEDELKSIKQKDLIEKVVDSARQAFKRKTEKMATVAYPVIKDIYEN
                     KGNQYESILIPITDGKRVFNITSKLEVAYKNEGKEIIKDFEKQTLLYVIDDEWKEHLR
                     QLDELRQSVQNASYEQKDPLLIYKLESFGLFKEMIDNINRKVLAILMRGQIPIREPEQ
                     VRQGEQQRRQDYSKYRTQKDDADRSSSSSDPTKQDTREPQKPEPIHVEKKVGRNEPCP
                     CGSGKKFKNCHGA"
     gene            18138..19037
                     /gene="lgt"
                     /locus_tag="PKH68_02835"
     CDS             18138..19037
                     /gene="lgt"
                     /locus_tag="PKH68_02835"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNX11903.1"
                     /translation="MLNYITWNVNPVFFSLGPLTVRWYGFLWAIGIFLALIIVQKLYK
                     NEKLPEAWIDKQFFYVVIGTIVGARLGHCFFYEWHLLPQPVEFLGISFKYGNPYLSHP
                     WKLLFIWQGGLASHGGAIGIIIAMYLYHRNVTHRGFVWGLDRLVIGAAATAFCIRLGN
                     LMNSEIYGSATTLPWGFIFVRDGQTEPMHPTQIYEMLYCLVTFLVTWWLYWKKAAYKR
                     TGLILGAFLLIVFGTRFLLEFIKLNQEAFESGMILNMGQILSIPFIVWGIWLIYDSKN
                     HIQPEEVAPAPSNPKNPKKKKQF"
BASE COUNT         5859 a         3938 c         3943 g         5313 t
ORIGIN      
        1 aaaaacttct ctacctgacc agtagagaag ttttttttat gaattacttc aacaattgaa
       61 tatattctct gagaatggcc agtgagtatt caccgccgta ctgattgaca aaaccaatag
      121 cactggtatg tgcctgctcg tcagctgtat cggatataat gcgcactacg gtgagcggca
      181 catcaaaatc gttgcagacc tgagctactg ctgcaccttc catttccgca caaactaccg
      241 atggaagatt tcggataagg gcttttttca tttcagtacc tgaaataaac agatcgccac
      301 tggcaatatc gccaagcatt agtttagggg ttggtgcatg ctgctccata gcaaaattcc
      361 ttaattgatt gttattcttc agaaaattat gtactgcttc aaccattaaa cctgaatttt
      421 gttcggagat ttcgtacgag gtttttcctg taaggggtat ttcgaatcgt cgcatcagcg
      481 ggcgggcatc catatcatgc tgaaacaggc gctgcgctat gactacatca ccgatgttta
      541 gatcatcgga gatggctccg gccacaccgg tgaacacaat ccgatccacg ccgaattcta
      601 caatcaggtt tgtagccgtg gtagctgctg ccacttttcc ccaccgcgaa aatacggcaa
      661 caacttcctg tccgtacaat ttaccccgat agtacaaccg gctcccccgc tccaccactt
      721 tcttatcagt cagcgcatca ataattttat ccatttcttc gggcattgcc cccatcaaac
      781 ctagtagcat aatatattaa ttagttgaaa tttatggaat aaataaaagt acatattgta
      841 aataaataca taaatgtgtg atgtcagagt agcgccttaa ttcgctgata caaagtggga
      901 tgtgaatagt gaacaaatac atacgcccga tgaggagtca gattggatag cgctttggac
      961 gaaattttct tcaacgacga aattagttga ttactcagcc cgttgacttt cacaaaggca
     1021 tcggcctgat attcaaaccg gcgcgaaagg atgttcatgc ccagaccaat caacattgcc
     1081 agcggtgaat agataaaacc aaatccaagc gcattaaggt ggaaggaagc ttctgacccg
     1141 cccaacgctt gtgccagagt ttggcttcgg agcatgatgc caaataagaa gaacatcagc
     1201 agggtgtagg gcatattaag catcatattt ttcagcgtat ggttatgttt gttgtgtcca
     1261 acttcatgtg ccagcacagc cacaatttca tcggttgtca aatccctgat tagggtatcg
     1321 tacaacacaa ttcgcttctt tttaccaaat cctgtaaaat aggcattggc ttttgtgctt
     1381 cttttagagc cgtcgattac aaatacattt ttcagtttga aatttgattt tacagcgaat
     1441 ttttctattt catcgcgcag ttctcccgct tcgagcggag tctgcttatt gaataatggc
     1501 actatcaatt cggaatagaa aaagctgata aacaagctga taactgtaat acaagaccag
     1561 gctatgagcc agaaataagt aggattaaga cgataaatcc acataaccag tgtaagaata
     1621 gcgcctccaa ccaccaccga aagtaagtat cctttaagtt tatcggtgat aaataatttt
     1681 ggtgtcattt tattaaatcc aaatcgctct tcgattacaa atgtttgata ccattcaaag
     1741 gggatagata tcaattcatt agccagaaat atcagaccaa aaaaaatgag cgaaactaca
     1801 ataggattct ctgaccaccc gcctaccaac tgactgagcc atccaaatcc acccgtacaa
     1861 aacataagca gtaccaccgt gagactaacg gccgaagtaa tcatgccaaa cttggtattt
     1921 gcctgaaaat aaagttgctg ttttcgatac tgagaatggg tataaacatc ggaaagcagc
     1981 gaaggaatag gctgggcggc agcttttgcg ttgagaaaag acaacgagcg ctccagtata
     2041 aaatcggcaa tcagaataaa aataatgagg aaaaaaagaa attgtaccac gaatattgaa
     2101 attttgattt attctacaaa gatacatttt ttagaggtaa aaaatgaaaa ccgatacaca
     2161 tattacaaga tgaagtatta tctttgctat aaggacaacc taaccacaat ccacctgaag
     2221 atggcaatgt acctcacatc cctgattgca tttacggcat taatactgat accggatttg
     2281 tggtttttta tgaaaatgaa aaaacatcat ttttatccct ggtttcgtct gttgagttta
     2341 gtacccggat ttctttttat actttccttt ttttatatcc gtttcggatt ggtgtacaat
     2401 tccagttatc aggttactgc cggaatatcg tggattttat tcgctttttc agtcatttac
     2461 acgcctaagg tactgtacat tgtgttttat tatatcaatc actcatttaa caggatattc
     2521 aatgcaaaaa cccgtatttt tcgcattatc ggctttgcat tgtcggtgta tatggttacc
     2581 tttctgtttt atggtgccgt tgttacccgc gatgattttt atttaaagac acaatatata
     2641 tatgtagatg agcttccacg taattttgac ggatataaaa ttgctgtgtt tgccgacctt
     2701 cacctgggca actggaacaa ccgttacaaa ataatggagc cgatcatcac tttgcttaat
     2761 gaggggaagc ccgatatcat tgtttttggt ggcgatttaa tcaacaatta tgagaatgaa
     2821 accgttgggt gggaaccata ttttagaagg ctaaaagcca ggcagggtat gtacgcggtg
     2881 ctgggtaatc atgattatgg cgactatacc aagtggaaaa cgaaggaaga aaaggaagaa
     2941 aatctggata aaacaaaaag aaatatccgc aatctgggtt tccgcttact gctcaatgag
     3001 catgtggaac tggtgaaagg atcggatacc attaccctgg caggcgtaga aaactacggt
     3061 tcaggtcatt ttgagaatca cggcgacctt acggcagcat tgaaaggaac caggcctgag
     3121 cgaaaaaaaa tcctgatttc acacgatccc aatcagtggg acgaagagat agcaacccga
     3181 aaaaaagaca tttttctcac actttcggga cacactcatg ccggacagat aggaattacg
     3241 aatagtaaaa ttcatctttc acccgcagct attatttatc caaagtggga cggactgtac
     3301 agccagggta accagtatct gtacgtaaac cgtggagcgg ggtatgtagg catcccattg
     3361 cgccttggag ttccaccgga aattacactg gttgtgctaa agcggaagaa gtgaaactac
     3421 aacttttttt ctgacaaaaa tcaatattct ctcattccaa aaatagaact tccaattcgt
     3481 accatcgtgc tgccttcgtc tatggctatt tgataatcat ctgacattcc catggacaat
     3541 tcgaggaaat tttcattgtt ctgaaaatat tctcgtttca atttgtcata cagatgtcgc
     3601 agggttctga attcagccct gatttgctcc ttgtcatccg taagagtagc catacccatc
     3661 aatccgttta tctgaacatt ctctaaagaa ccgaattcat tgcccgaaag catttggcga
     3721 atttcttcca ttgaaaaacc gaatttggtc tcttcccggg ctatatgcac ttgcagtaag
     3781 caggatacta ccctgcccgc tttcagagcg cgggagttga tttctttgag cagattgaaa
     3841 ctgtccactc cgtgaataag acttaccgtt ggcagtatga gtttgatttt atttgattgc
     3901 aaatgtccga taaaatgcca ttgaatatcg gccggaagtt tagtttgctt tgcctgtagt
     3961 tcctgcacac ggctttcacc aaaaattcgt tcgccacagt tgtaggcttc caatatcgca
     4021 ttttcgggat gaaatttgga cacacacacc aaagaaacat tcgcgggaat gttttttctg
     4081 atttcctgaa tattttgtgc gacactcatt tgtcccaaag tctggtgatt aatttcttac
     4141 agaattctgt tgtcttattc tgctttatag gggaatacat ccataatatt tgtttcggta
     4201 attgcggcga tttcgtaatc ggccagcgta cccttcatcc ctttttcaat cacctccagt
     4261 gcatctttta ccgaacttgc ctgagcgtac atcgttgtgg caattcgttt ttccacccct
     4321 ttttcttcgt caagactgat aaagttcact tttgagcggt accagcgatc accggcttcg
     4381 ttggcgaaaa gttcgttaac tttttcccgt ttgatgtctg acaccagaaa ttcaccgctg
     4441 ataaacggac gcatttcttc gttgatacgc tcttcagcct ctgtgaacga cagagcatca
     4501 accagatacg cttcacttgt tttaactatg ccaccatcct gggcattttt ttcgtacttc
     4561 actttacact gaaaccaatt atgcatttct gttaattatt taattatgag tatattaata
     4621 tatgttttat agccttttga ccggatacaa aagtaacaaa aaaacaccaa acagaaatca
     4681 tcataaatct caaaggatta aaaatattct ttaactaaca taaacacagt gtgtaatttc
     4741 tgaattttat ctgaataatt ttttttcgta cctttgccga aaatttggga aacatgggca
     4801 gagtgatggc aatagattat ggcacaaaac gcacaggact ggcggtaacc gatcccatgc
     4861 gcattattgc caacggactt gatacagttc ccacacacga agttctcgat ttcctcgaaa
     4921 aatatgttgc gagagagcct gtggatattt ttgtgttggg attgcccact cagatgaatg
     4981 gcgaagagtc cgaaacgatg aaacaaatca agcctttcgc gcataaatta caaaaaaaat
     5041 ttccaaccat tgaactcatt tatgtagatg agcgttttac ctcggcactc gctcatcagg
     5101 ccatgctgac cggcggatta aagaaaaaag atcgtcagaa taaagccctg gtggacaaaa
     5161 taagtgctac catcattctt caaagctatc tggaaaataa aattaactta ctaaaatgat
     5221 actacccata tatacattcg gcaattcggt actccgaaaa atagcagaac cgatagacca
     5281 ggattatccc ggtctgaaag atttgattaa aaacatgtac gataccatgg tgcatgccga
     5341 aggcgtaggc cttgctgcac cgcagatagg tattcccatt cgtttgatag tgatagattt
     5401 ggcaccgctg aaggaagata atccggaatt aggcgatttc aaagtgacca tgatcaaccc
     5461 tgagttgctg gaggagagcg aaaatgaagt ggaatatgac gaaggctgtc tgagcattcc
     5521 gggcatcaat gaaacggtgt cgagacccga agctataaaa atcagctaca tggacgaaaa
     5581 tttcattgaa cacgtggaag tttatgatgg ttacaaagct cgcgtagtgc agcacgaata
     5641 cgatcatctg gaagggcatc tgtttaccga caagataaaa ccgcttcgcc gtcaactaat
     5701 taaaggcaaa ctgacaaaca ttgttaaagg aaaagcaagt tgtaaataca aaataaaata
     5761 gcacactaca catacgaaaa acgggatgaa tattaaacaa ttcatcccgt tttttcttag
     5821 ttatatttcc ttattttttc tgctctacgg catttccttc ttttttatct tcggattctt
     5881 cagccttttc cctgattttc aattcaggaa tgtaatgatt agttacaatg ggtttagcta
     5941 ctgatccggg tatgtaatca cgccggtaca tattgaaaaa caatataaat aacgaaagca
     6001 gcagcggtcc aaatatcact ccccaaaacc caaacatggg aataccgata aaaacgccaa
     6061 aaatagttat caacggatga atatcagcca gccgtttttg cagtatcagc cgggctaaat
     6121 tgtcaacacc gccaataacg atggccccga aaagaataat tccgatgcct cccacataat
     6181 tggcctgaaa taagtagatt acgcccagcg gcagccagat gagcgcagtt cccaccaccg
     6241 gcaatatggt ggcacaggct gtgagtacgg cgtacagcag tgcattgtcc aaaccgaaaa
     6301 tcatataacc aacgtaggca aatattccct gaataatggc cagcagcggt attcctacgg
     6361 cattggcctg gataatggac tttgtttcat gaacaaacga ctgtttgttg tcgtgagtaa
     6421 aaggtaaaat ttcacgtatt gtaatttcga attcctcgta actgtagagc ataaaataca
     6481 aaacaaataa aattaccact acattgataa ccagcgaata ggaactggta gccagcactt
     6541 gaaccacatt tgtacccaat ttgggaataa atgacagatt ctcgggagtg aacagtttga
     6601 aattaaagcg attttctatc aggtttacaa agttaagtag ctgttcttta atgatttcag
     6661 gatcaatttt gatgcccgaa agtgtgtcga tggctaaaaa tgtaatacct gtcaggggaa
     6721 tcaaaaatac cagtaacgtt tcaagcagaa tgatcgtagc acttaatcct ctattcagct
     6781 ttttcttttt agtcaggtat ttcatttgtc cacgcaagag tacaaacaag gtgattgcgc
     6841 ccataaaacc actcagataa ggttgtagct ccctgaagag tataaatccc agaattaaaa
     6901 tcaaggctat gagtatgtac ctgtactcct ttttattcag ttgttgttct gtcatagtgt
     6961 gtcatatttg ggtcttggaa ttatcaaaat cagagaatat cctgtggata tcagtttgta
     7021 aaattacaat aaaattcatt ttattcaaca gaaaacgtat aattgtttta ttagctaaag
     7081 acgaaagaat atttagttaa aaaaatccga tatattttaa ttgttgttct tttgctgtga
     7141 atattttcga tggcaggttg atatacaagc aattattaag tccatattat tatttgtaaa
     7201 aattaatttt ctcaaactat tcccggtgat tgaatttcaa attggaaaac tttttgttaa
     7261 aaataaaatt ctatatatca aatacacaag taattagaat ttattttgga aaacttttca
     7321 gaaatattga aaatatattt tgttattctt tggataatat caaattattt tcttaaaatt
     7381 gtagtccgca aaaaccaaaa tcagaaattt cataaacacc taatttataa catcctattc
     7441 aatcgtggaa agaaaaattt acactcaggc cgaagtcgag aatgcaacca gagactactt
     7501 caaaggagac gaactggcag caaaggtatg ggcaaccaag tatgcattga aagactcgtt
     7561 cggcaacatt tacgaaaaaa ctcccgatga catgcaccgt cgtctggcaa gtgaaatcgc
     7621 aagaatcgaa gaaaagtatc ccaatcctct ctcgaaagag gaactttttg aattgttgag
     7681 ggactttaag tacattgtac cccagggaag ccccatgaca ggtatcggca atgaatatca
     7741 ggttgcctca ttgtccaact gttttgtaat cggtttcgac ggagactccg attcatacgg
     7801 tgccatcatc aaaatagatg aagaacaggt acagctaatg aaacgacgtg gtggtgtagg
     7861 tcatgacctt tcgcacatcc gccccaaagg ttcgccggtt aaaaactctg ccctcacctc
     7921 taccggtctt gttccattta tggagcgata ttccaactcc acccgcgaag tggctcagga
     7981 tggccgacgt ggtgctctga tgctgagtgt ggctatcaaa caccccgatg cagagtcatt
     8041 tattgatgcc aaaatggaat cgggaaaagt aaccggagcc aatgtatcgg taaaaattga
     8101 cgacgaattc atgaacgccg ccatcaacaa catcccttac acccaacagt atccgttgta
     8161 cggaaacaat ccgtgggtca aaaaggacat tgatgctgct gcactgtgga aaaaaattgt
     8221 acacaatgcc tggcaatctg ccgagcccgg ggttcttttt tgggatacta ttctcaaaga
     8281 atctgtcccc gactgctatg cggatctggg ttaccgaaca gtttccacga atccctgtgg
     8341 agaaatacca ctatgtccgt atgatagctg ccggttgctg gccatcaata tgtattcgta
     8401 tgtcaacaag ccatttaccc ccgaagcctg gtttgatttt gatcttttca gcaaacatgt
     8461 acagctggct cagcgcatca tggacgacat catcgatttg gaaatggaaa agattgaccg
     8521 gataatagct aaaatcgaat cggatcctga agatattgat attaaaaaca ccgaatttca
     8581 gctgtgggaa aaaattaaaa ccaaaacatc gcagggacgc cgcaccggtg tgggtaccac
     8641 cgccgaagga gatatgctcg ctgctatggg tcttacctac ggcaccgatg aggccattaa
     8701 attttcggaa aaggttcata aagctgtagc acttgccgcc taccgctctt cggtgaccat
     8761 ggccaaggaa cgcggtgcat tcagcgtgta tgatgccaaa cgtgaggaaa acaacccgta
     8821 tataagcaga attcgtgaag cagatccggc attgtatgaa gacatgacaa aatacggacg
     8881 cagaaatatt gcgtgcttaa ccatagcacc tacgggtacc acaagcatca tgacccaaac
     8941 cacttcggga atcgaaccgg tattcatgcc ggtttacact cgcagacgca aagtaaatcc
     9001 gaacgataaa aatgtacgta tagattttgt ggacgnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnncgcag acgcaaagta aatccgaacg ataaaaatgt acgtatagat
     9181 tttgtggacg aaaacggtga ttcgtgggaa gagtttattg tatttcacca caaatttgtg
     9241 acctggatgg aagctaacaa ttattcaacc acaaaaaatt acaccaagga ggaggtggat
     9301 gaattggtag ctaaatcgcc ctattacaaa gctaccgcga atgatgtgga ctggatgaaa
     9361 aaggtgcaga tgcaggggag aatacagaaa tgggtggatc actctatcag tgttaccatc
     9421 aatttgccaa acgatgtttc ggaagagctg gtaggaaaac tctacgagga agcatggaga
     9481 tcgggatgta aaggtgcaac tgtttatcgc gatggttccc gtgccggagt tctggtagca
     9541 atggaagaga aaaaagaaga aaaaaaggat gataaggaac gctgtttttg ctacgaagac
     9601 gaacgattgg tacgtcccag ggaactgaaa tgcgatgtgg ttcgcttcca gaatgcaaag
     9661 gacaaatgga ttgcattcgt aggactgtat gacggacgac cgtatgagat ctttaccgga
     9721 ttagccgacg atgaggaggg aattgtactg ccaaagaacg taacagaagg gaaaatcata
     9781 aaagtggtgg acgaaaaggg taaacggtac gactttcagt ttaccaacaa aagaggtttc
     9841 aaaaccactg ttgaaggact ttcgcataaa tttgacaaag aattctggaa ttatgccaaa
     9901 ttgatttcgg gtgtattacg ttacggaatg cctattgacc aggtcattaa attagtatcc
     9961 ggattgcagc ttgacagcga atccatcaat acctggaagg tcggtgtgga acgtgcgcta
    10021 aaaaaatata ttcccgatgg aacgcaaatt tcagaacagg cctgcccgca ttgcggacag
    10081 aaatcactgg tgtatcagga gggttgcctt acctgtaaga attgcggtta ctcgcgatgc
    10141 ggttaatcag ctgatattta gctattcaat aaaaaagaca ggttatcagc ctgtcttttt
    10201 tattattgca tattcgccgt ttcttcattg ggcgaaattt cactgtattt tttgtgcctt
    10261 cggatgtaaa attcatatat cacgctgtgt tgggaaattt ctttttgcag tgaattcacc
    10321 gatttgagga tattctcctt tcgttgatcc gaaaatttag actgaataat tctgaattct
    10381 gtacgggcat ctgttactgc ttttgcattc tgatattttc cctgcaggag cagatgcaat
    10441 gcagccagat tgtccagata aaaccggaca tcaaatacat gcttaatatt tttctggggt
    10501 aaatttttgt aaagcatgat caggttgttc ctgaaattaa ggaaagtttt tcgggggtgt
    10561 tcggcattca aagtgcctcc tccaatgtga taaacaacac tccggggcac acagactatc
    10621 cgttttccgc gacttttcag ccgccagcac aggtcaattt cttccatgtg agcaaaaaac
    10681 tcctcatcga ggcctccggc atcccaataa tcggccgaac gaataaacat acaagcaccc
    10741 gaagcccaaa aaacatcttt aatgtcgtcg tattggccca aatcttcttc tacctctccg
    10801 aatatccgtc cacgacaaaa gggatatccg tacttgtcga taaatcctcc cgctgccccg
    10861 gcatattcaa aataatttcg ccggtgataa gaacggattt tcggctggca ggcagcaaca
    10921 tccggatttt tagtcataaa ttcataaaga gctgatagcc agtctttggt aacttccaca
    10981 tccgaattta gcagaacata atattcggaa ttgatgttct ttagtgcttt attgtatccc
    11041 ccggcgaatc cgtaattttt ctcaaaagca tccactttca ctgtgggaaa tttttcggcc
    11101 aaaagttgca gcgaaccatc ggtggagcca ttatctgcta caacaatttc agcatccggt
    11161 acattatcca gattattcag aaggataggt aaaaattcat tcaggtactt ttcaccattc
    11221 cagttaagta ttactaccga tacattcata taagatatcg ttttttttta tttatcagca
    11281 aatttccacc tgcggtgtga ccaaagccag taggctggtt cagccattat ttcctgttcc
    11341 agtaagcgga tgtatttctc tgtaatttca aattttccac tttcctgagg atgcagacat
    11401 atgggtaccg gattacaggt ataatatccc cgtttcactc tgcggatttg gacaaaatac
    11461 accggaaaat cgtatttttt agctattatt tctgtgcccg taattacagg agtacgttgg
    11521 ttcagaaact gagattgata tttaatccct tttcgggacg gactttggtc cgaaataagg
    11581 cctatcattc ccatttttcc ctgttgtttt aatgatgaca ggacatcaaa tagttcattt
    11641 acttccacat tcacagagcc aaagcgaatt cgtaaattat aaatcaaccg gtcaaacagc
    11701 ttgtttttct gttgtttata aacctgatac atcggtttat cagccggaag atacatgctg
    11761 aatgacgaag tccattccca gttgccgtaa tgcgaagtga tgagcaatac acttttattc
    11821 tcatcgtaat gtttgagaag gggctcgtaa ttgagtattt tcatcctacg gctcatctcc
    11881 tctttgctga tagtggccat tttcatactt tcaaacagca aatcgcaaag atgatggtaa
    11941 aacttctttt ctgtggccag taattctctg tccgttttac cgggaaatga ccgtttcaaa
    12001 ttctccctga ccacattttt tcgatatcct atcagataat atactatcag gtaggtaaaa
    12061 tctgacagga cataaagtat cgaaaatggc aaaagagatt ttaatttcag cagcccgtta
    12121 ataatatgat acatggggat taattatagc caaaaaaaca cccgacaaaa gaatgtcggg
    12181 gtgcttatat tgtattctgt agatgatttt attcttcttc ggaatatatt ccgagtgatt
    12241 ttccatattc atactcaccg tcgagtacca gctgaatttc atcttcggtg agattcattt
    12301 tatctttttt ggaagctttc agcacaaatt tcatcatttc ttcttcatca atgctggctt
    12361 cttcggtcga ttcttcatca atcaaaccat tttcttcata atattcataa actacatcca
    12421 acacataatt gatgacatcg tcgttgattc gggctttctg ttcatccgga atcagtttca
    12481 gaataaattt tacggcatcg tcttcgtcgt aaaccagtaa atcgtcgtct tgtgttttca
    12541 taattataag tatttatttg tttttgaatt tctgcttaat tgcgtttcaa agataaatga
    12601 ttttcttaac acaatctatt tttttagttt ttattattaa aaaagccgtt tctaagtacc
    12661 agttcgtatg attgtattat agcggtatta tcagtcaaat ttggatatta tttaaaaaaa
    12721 cgcttatctt tgtatattaa tagcaggctg gtttgtcgct gttccgctca cgcggaattg
    12781 aggaaagtcc gggcaacgca gagcaccata tttcctaacg ggaagctgct tgtgagagca
    12841 gagtagtgta acagaaaata accgtctccg aaacacatta ccttattccc tctccgtttg
    12901 gagagggtgc ccgaagggcg ggtgaggtgt agggataagg gtgaaaaggc ggggtaagag
    12961 cccaccggtt gtgatggtga catcgcaagc cgtacacctt atgggttgca agatcatgta
    13021 taccggcgca gtagggctgc tcgtccgatg ccggggggta gattgctaga tttcgtcagc
    13081 aatggcgaaa ccagataaat gacagacact ccgatttttc ggagtacaga acccggctta
    13141 caggcctgct ttttttataa aattccgggt gcatattaca tgcactttca attcatatcc
    13201 gatgaaaaaa ctgattgaaa ccatcaaata tctgtacaat tttatattgt atgatatctg
    13261 gcgtataacc ggcaacgaac tgacccgaag ccgtcgtttt ttctacggag ttattaagac
    13321 tatttatctg gcattcaggg gttacagtcg caataaactt agtgtgagag cagcagccct
    13381 cacctactcc attacatttg ctattgtacc gcttatcgca ttgatggtaa caattgccaa
    13441 aggttttggt atcgagagtt ttatcgaaaa aacgctgcag gataccttta tagcgcaagc
    13501 taaccttatt ccggctacca tggggtttgt ggaaaaatac ctggaaacca tgcagggagg
    13561 tctttttatc ggtattggcg ttgctatttt gctttactcg gtcatgaatc ttttcatgca
    13621 gatagaagcc acgctcaaca atatctggca ggtacaaaag tccaggtcca tattcaagca
    13681 atttaccacc tattttgctg gtgtggtttt gtttccctta cttctgacca taaccagtgg
    13741 tctgtccatc tacatcaacg gtatacttaa aaacaccttc atctatcaga tatacagtcc
    13801 gtttgaacag ttcacagtgg cgctggttcc gtacctgacc ggtattctca tgtttaccat
    13861 tatttatctc attgtgccca atacgcgtgt ccgctttgtg aatgcgctta ttgccggtac
    13921 agttgccgga attgccttcc agcttttcca gacactgtat gtcaacgggc agatcagctt
    13981 aacgcgctac aatgctgtat atggaggatt tgctgccatt cccctgttgc ttatctggct
    14041 gtatgtgtcc tgtctgattt ttttggtagg agccgaaatt tcgtacgttt cccaaaatct
    14101 tcgtaacttc gactacgaag tggataccga taatatcagc aaccggtata aaacgtatct
    14161 tgaatttttt gtagcctaca tcattgtcaa gcggttcgaa aatgataaac ctccaatgac
    14221 tgccgaggat attgtgaatg aatataaact tccgatacgg ctgctgaacc aaattttatc
    14281 cattctgaca aattcaaaaa taataatcgc tgtaaaacag gataacaacg aaactgccta
    14341 tcaacctgca ttagacatta atctactgac aatgaatatg atgtattcca aaattgatct
    14401 gcggggttcg gatatgtttc tcaatttgaa aaatgatgag atggacgcat tctggcataa
    14461 aatgcaggaa cttcaggcta aaaccgacaa aatacgcgaa aaaatactac tgaaagacct
    14521 ttaattgctt attgtctgaa atttttactc atcctgaatt gcaattactc acaacttata
    14581 aacacttttt ataggtcagt agtactgtta aattgcaaaa aaacagctat ctttgcagga
    14641 atttgcaaaa aatcaataca aaaaatcaat cagtatatta ctaatcggag ttatactgat
    14701 attcaacaag ataactttat taaaaacgat acagatttca gaaatttcac aagaatagct
    14761 gaaatgaaat caacaaaaaa ttattagaaa tatgggattt aacgatattc tgaaaaagat
    14821 agtaggcagc aaagccacgc gcgatatgaa agaaatatcg ccggaagtta ctaagataaa
    14881 ggctgtatat gaacaaatta aaaatctgag caacgatgaa cttcgcggac gtaccgagct
    14941 gctcaaagcc agaattcagg aagccatcgc tcccgaaaat gaacgtattg aacaacttaa
    15001 agccactata gaggaaaccg aactccacct tcgtgaaaaa atttacaacg atgtggataa
    15061 actggaaaaa gaaattgtag agaaaatagc cgttgtgctc gacgagatac ttcccgaggc
    15121 attcgccata atgaaagata cggcacgccg actgaatgaa aacagtgatt tggttgttac
    15181 cgccactcaa ttcgaccgtg atctggctgc aaagtacgac tttgtatcta ttgaaggaga
    15241 taaagcacac tttaaaaatg aatggattgc cgggggcaac ctgataaagt gggacatggt
    15301 gcactacgat gtgcagttgt ttggtggtgt ggtgcttcac aagggaaaaa ttgccgaaat
    15361 gggaaccggt gaaggtaaaa cactggtggc cacccttccg gttttcctca acggactgac
    15421 acacagaggt gtgcatgtgg taactgtgaa tgactacctg tcaaaacgtg actccgaatg
    15481 gatgggacca ttgtatatgt tccacggatt atcagtagat tgtatcgaca aacatcaacc
    15541 taactccgaa caacgccgtc aggcatacat ggccgatatt acctttggca ctaacaatga
    15601 attcggtttc gactacctgc gcgataatat ggccaacagt ccgatggacc tggtacagcg
    15661 caagcacgtt tatgccattg tcgatgaggt tgactccgtt ttgattgacg atgcccgtac
    15721 tccattgatt atttcaggcc ctatccccaa gggcgaagag caattattcg aagagctttg
    15781 tcccaaagtg gaaaaactgg tgaatgcaca gcggacactc gtaaccaaat acctggccga
    15841 tgcacgtaca ctgatgaaat ccgacaagga aaaagatcgt gaggaaggca cactggcatt
    15901 gttccgttcg tataaaggga tgcctaaaaa caagcccttg ataaaatacc tcagcgagca
    15961 gggtgtaaaa gctgccatga tgaaagtgga agagatatac atggagcaaa acaaccgtcg
    16021 catgcccgaa gctaccgatc cactgtactt tgtgatagat gaaaaaaata attcgataga
    16081 acttaccgac aagggtattg acctgataac cggaaattct gatgaccctt actttttcgt
    16141 actacccaac gtgggtgacg aaatagctga actggaaaaa atgcccggtt taactccaga
    16201 tgaaaaacaa aataaaaaag acgaaattct tcagaattat tccattaaat ccgagcgtgt
    16261 acacaccatc aatcagttgc tgaaagcata tacattattc gaaagagaca ccgaatatgt
    16321 ggttcttgaa aataaagtaa aaattgtgga cgaacagacg ggtcgtatta tggagggacg
    16381 ccgttactcc gacggcttgc atcaggccat cgaggccaag gaacgtgttc aggtggaagc
    16441 agccacgcag acatttgcca ccatcaccct tcagaattat ttccgtatgt atcagaaact
    16501 gtcgggtatg acgggtactg ccgaaacgga agccggtgaa ttgtggaata tctacaaact
    16561 cgacgtggtg gtgatcccca ccaaccgccc cgtagtgcga aatgacatgg aagaccgtgt
    16621 gtataaaacc aaacgcgaaa aatacactgc tgtcatagaa gaaatagtaa aactggttga
    16681 agccggacgt cctgtacttg taggtaccac atccgtagaa atttcggagt tactgagccg
    16741 tatgcttacc agcagacaca ttaaacacaa tgtactgaat gccaagctgc accagcgtga
    16801 agccgaaata gtagccgaag caggtcaaaa aggcactgta accattgcca ccaatatggc
    16861 cggtcgtggt accgacatca aactgacccc ggaagtaaaa gatgccggtg gtttggccat
    16921 cattggtacc gaaagacatg aaagccgacg tgttgaccgt cagttgcgtg gtcgtgccgg
    16981 tcgtcagggt gatcccggtt cctccgtatt ctacgtttca cttgaagatg atctgatgcg
    17041 attgttcggt tccgagcgaa tttccagtgt tatggaccgt ctgggatttg aagaaggaga
    17101 agtaatcgaa cactccatga tttcgaaatc catcgaacgg gctcagaaaa aagtggaaga
    17161 aaacaacttc ggtattcgta agcgattgct cgaatacgat gacgtgatga actcccagcg
    17221 cgaagtcatt tatgccaaaa gacgacatgc actgatgggc gaacgtatcg gtgtggatat
    17281 ttcgaacatg atatatgatg gcgctgaaac cattgtggaa actgcaaaaa attcggaaga
    17341 tttcgaagga tttgaaatgg acctgatgaa aatatacgcc atggattctc ctatcgaaga
    17401 agacgaattg aaaagtatca aacaaaaaga cctcattgaa aaagtggttg actcagcccg
    17461 ccaggctttc aagcggaaaa ccgaaaaaat ggcaaccgtt gcatatccgg tcattaagga
    17521 tatttatgag aacaagggaa accagtatga aagcatcctc ataccgataa ccgatggaaa
    17581 acgggttttc aatatcactt caaaactcga agtagcttac aaaaatgaag gaaaagaaat
    17641 tatcaaggat tttgaaaagc aaaccttgct ctatgtaatt gatgatgaat ggaaagaaca
    17701 tttgcgccag ctggacgaac ttcgtcagtc ggtacaaaat gcgagttacg agcagaaaga
    17761 tccgctgctt atttacaagc tggagtcatt cggacttttc aaagaaatga ttgacaatat
    17821 caaccgcaaa gtacttgcca ttcttatgcg cggtcagata cccatccgcg aaccggagca
    17881 ggttcgtcag ggtgagcagc aacgccggca ggattacagc aaataccgca cccagaaaga
    17941 tgatgccgac agaagttcat catcatcaga cccaacaaag caggacaccc gcgaaccgca
    18001 aaagccggaa ccaattcatg ttgagaaaaa agttggacgc aatgaaccat gtccttgcgg
    18061 aagtggcaaa aaattcaaaa actgtcacgg agcttagcga ttcatataac taattcataa
    18121 tcaatccatc agatatgctg ttgaattata taacctggaa tgtaaatcct gtatttttct
    18181 cactgggacc tttgacagtt cgctggtatg gttttctgtg ggctattggg atttttttgg
    18241 cacttatcat cgttcagaaa ctgtacaaaa acgaaaaact tcccgaagcc tggattgata
    18301 aacagttttt ctacgtggtc attggaacca ttgtaggagc ccgactggga cattgttttt
    18361 tctatgaatg gcatctgctg ccccagccgg tggagttttt aggtataagc tttaagtatg
    18421 gtaatccata cctgagccac ccctggaaat tgctcttcat ctggcaggga ggattagcca
    18481 gtcacggagg tgccattgga attataattg ccatgtatct gtatcataga aatgtaactc
    18541 accgcggatt tgtctggggg ctcgaccgcc tcgtaatcgg agctgcggcc actgcttttt
    18601 gtatccgact gggcaatctg atgaattcgg agatttacgg cagtgccact accctgccct
    18661 ggggcttcat ttttgtgcgc gatggtcaaa ccgaaccgat gcatcccaca cagatatacg
    18721 aaatgcttta ttgcctggtc actttccttg ttacctggtg gctctactgg aaaaaagcag
    18781 cctacaaacg aacaggactg atactcggag catttttatt aattgtattt ggtacccgtt
    18841 ttctgcttga gttcatcaaa ttaaatcagg aagcattcga aagcggcatg atactgaata
    18901 tgggacaaat actgagtata ccgtttattg tctggggtat ctggttgatt tacgacagta
    18961 agaatcatat tcaaccggaa gaagttgctc ctgctccatc caatccaaaa aatcccaaaa
    19021 agaagaaaca attctaacca tactgattta agccttgcct gcaaggctta ttttattaaa
    19081 aaatattgga aatggatatt aacaacgtaa aaccatataa ccaggaagag gaaaaaaccc
    19141 gtcagctgaa ccg
//
LOCUS       DAOPZK010000026        18838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3064_length_18838_cov_22.526593, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000026 DAOPZK010000000
VERSION     DAOPZK010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18838
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_18838_cov_22.526593"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKH68_02840"
     CDS             <1..242
                     /locus_tag="PKH68_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNX11904.1"
                     /translation="CGGVNVDRLKKVKKMPIRMFHGGADPVVSREHSRNAFIELKAEG
                     STRVEYVEFPGVGHDSWTNAFEYPDFLSWMYYQSR"
     gene            393..629
                     /locus_tag="PKH68_02845"
     CDS             393..629
                     /locus_tag="PKH68_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX11905.1"
                     /translation="MAKAKGAVVVNTERCKGCNLCVVSCPWDVLSLNKEANSKGYNYS
                     YMLAPENCVGCATCAYICPDACITVYKVKAEKTV"
     gene            750..1832
                     /locus_tag="PKH68_02850"
     CDS             750..1832
                     /locus_tag="PKH68_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNX11906.1"
                     /translation="MEEIILMKGNEAIAHAAIRSGCDGYFGYPITPQSEVMETLMELK
                     PWETTGMVVLQVESETASINMVYGAAGCGKRAMTSSSSPGISLMQEGMSYMAGAELPA
                     VVINVMRGGPGLGTIQPSQADYFQTVKGGGHGDYKVIALAPASVQEMADFTVLAFDLA
                     FKYRVTCMLLADGVIGQMMEKVQLPPYRPRWTEEEIRQQNPWGTMGKTNDRKPNVITS
                     LALQADEMEVINLRLQARYREIEENEVRFEKIMCDDAEYLVVAFGISARIGQKAVELA
                     RQEGYKVGLLRPITLFPFPTVEISKMADQVKGMLTVELSAGQMVEDVRLAVNGRVPVE
                     FYGRMGGIVPSPDEVLRALKEKVLGK"
     gene            1865..2626
                     /locus_tag="PKH68_02855"
     CDS             1865..2626
                     /locus_tag="PKH68_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX11907.1"
                     /translation="MTTEEIVNPANLVYKKPSVLNDVNMHYCPGCSHGVVHKLVAEVI
                     DEMDIQNQTIGVAPVGCAVFAYKYLDVDFQEAAHGRAPAVATGIKRLLPDRYVFTYQG
                     DGDLAAIGTAETIHACNRGENIVIIFINNGIYGMTGGQMAPTTLIGMKTATSPYGRTA
                     ELNGYPLNITPLLAQLEGTCYVTRQSVHNVANIRKTKKALRKAFENSMMNKGTSVVEV
                     VSTCNSGWKLSPEASNDWMVENMFPEYPLGDMKDK"
     gene            2754..3299
                     /locus_tag="PKH68_02860"
     CDS             2754..3299
                     /locus_tag="PKH68_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNX11908.1"
                     /translation="MKEEILISGFGGQGVLSMGKILVYSGMMQGQEISWLPSYGPEQR
                     GGTANVTVILSDERISSPILHNYDTVIVLNQPSLDKFESKVKPGGTLIFDSNGFKKFP
                     TRTDINVYRIDATEYAYAHDSSKTLNMIILGGFLKIRPVVKIENVLKGLKKSLPERHH
                     HLIPQNEEAIKVGMSLIQKYN"
     gene            3611..4192
                     /locus_tag="PKH68_02865"
     CDS             3611..4192
                     /locus_tag="PKH68_02865"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNX11909.1"
                     /translation="MNLINRVKSILLTPKTEWPVIETETTDVKTLTVNYLLPLALITA
                     VATFIGYGLIGQNVFGVHIGSAGFGLRYALVSLVSTIGGAFLTAYIIDLLAPSFGSEK
                     NFLQSFKLVVYSYTPMLVAGILLIIPSLGILATLAGLYGLYLLYIGLQPMKKTPEDKK
                     TSYFVVSLIVVILGSVVLSAILSSIFITSGIRY"
     gene            4270..5568
                     /locus_tag="PKH68_02870"
     CDS             4270..5568
                     /locus_tag="PKH68_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX11910.1"
                     /translation="MKRIVFFLCVSFLFSLQLSAQGWLERAGQRVKDRVVEKVENRID
                     KKTDEATDKVLDKAEGSVKKSGKKNKQGQQDAEEAAETDENQDENAAPKGKTAQKLES
                     VSAYDFVPGDKILFFEDFSQDAIGDFPALWTTNGSGEVKTVNIAPGKWFHMNGDDAVY
                     CYTKEINFPDNFIVEFDIIPEKNFQYGICLILYQEDPENPKEVNDDLFPGLRGLRIAP
                     NSDGWETNGYESDPNKPWLDGKGSKNPLIAEELNHVILWIQKRRVRIYHQGQKVLDMP
                     TNIYADTKFNRLLFSGWDRNCAPLVSNIKITTASPDTRSKLITEGKLITYGITFDVNK
                     ADVKPESYGTLKSIADVLTENPGVKVKITGHTDSDGDDAKNLELSKRRAESVKNELVK
                     NFGIDASRLSTDGAGETKPIAANDTPANKAKNRRVEFIKL"
     gene            5655..6704
                     /locus_tag="PKH68_02875"
     CDS             5655..6704
                     /locus_tag="PKH68_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11911.1"
                     /translation="MKKSVVIILFLSTAVTSLVTAQKTALEYLKTVPGNPDSVCVCLK
                     EVRQNYETAVSELYDIVKEDIQSRTRAVEDYMDQNQDRMKASMMKEMSEKTGMSQQEL
                     ETLSKKKNLSKEDKQALMDKALGQHNLSMGEVQNMKNMDANARKAWAEAYATEQMAMA
                     QGNSKNPQVKVNNDKAMGQVALMQEQQTVRNQLSTFETETANRYKTLIQTSGKEKMAA
                     DLQKLENELRTIGSIYECNECPPPPAEDVKHWNAVMKKIHDTKIQYCRLNTPKMIAYL
                     NWYRENLVKNIPLYDKSEELQYKLTSATTNTQLTPPDKGIFSLQAVQGFIGAKQKLFE
                     FKDYEIENFTYDFIK"
     gene            6756..8948
                     /locus_tag="PKH68_02880"
     CDS             6756..8948
                     /locus_tag="PKH68_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX11912.1"
                     /translation="MKSYLTIFFLLISLSLAAQSTNADEIRRQMAKVRQSTNWSDPAA
                     ARKATEEIRRLAAKLSGNQNLPVIGGSDNSTPLGKQTEVKSSVNPTKENILRIAERFY
                     NRSYKQLNAIEKSRFDLEYQNAQKNKFNRQAVNALTSNGANLMMFGTNHHEGCVYLTS
                     AVKADINDTLAVNNFGAYLRIIDSLKVSLPVLLYANTLFDKSPVILTQIGCSYFELND
                     FKKGETYLKLALQVNPGFGKAHSALCELYLKTGRWKEALQELIAGVVNQGCSYMQAHA
                     NFQQLADVSSANKSGGSAKAQGFYNQNNLSDDDALAPLVPENKGKVQIDPKDMLASLV
                     PEDNIPDEDKLAPLVPQDNKIKMPQFANITSVNDWMEGGGYSGAISAFQSFLTTDMAA
                     ANELQQVLKEQPNISPDAVLRDYPNERFAIDCITEMIGDMSNDESMKLSKKTDKYIEQ
                     AVSYQQIYMDKYQRISKDYMSCLQACPTENPMHDYCTGECQRKYCLEMCNAANSYNQN
                     LQNIFDDWTRDFNESKQKQQELLDDLYGFTTPWFKKLYSPYWSRIEAYEIRRVALLIV
                     GNTVTGYAHRALTFPAHTDCGSDCSVFVNAAPPPVEEVDKKEPNGNECPFEGNKLSLG
                     IGLCSVDFDCESIEAGCSAGVSISAKRNFKNKSSTLFVGAGGEANLGAVSGEIKTGVT
                     FTKYDNGNMDVGGKFEMTGKVGPVGKNYEMSATLMEGANVEGKNVIGL"
     gene            9039..9698
                     /locus_tag="PKH68_02885"
     CDS             9039..9698
                     /locus_tag="PKH68_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11913.1"
                     /translation="MKNSIFILSIVFVLAACGQKPADKAATDEKSATETVTQVKKGKK
                     YPIEKGIVYQKSDVMGIVSNPVIYFDKWGEWEATETTTSIEMMGIKESSTSLKIVKGE
                     DNWEIDMDKKTGFHYNRAVMINQMGVDMENLTTEIMGKMKLEKVGEEKYLGYPCVKYK
                     MKNDEMKMDVEYLMCGNLMMKMKGKAMEIPTELEVTKIEAVTPPAEKFEIPAGVKITE
                     Q"
     gene            9891..13118
                     /locus_tag="PKH68_02890"
     CDS             9891..13118
                     /locus_tag="PKH68_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNX11914.1"
                     /translation="MKNFRLLNNIFGWVAFAVAAVTYLLTIEPSASFWDCGEFISSAY
                     KLDVGHPPGAPFFMLTAKFFTLFASDPTQVAMMVNSFSAIASAFTILFLFWTITHLAQ
                     QVLGKSEEEYSAANIIGILAAGMVGALAYTFSDTFWFSAVEGEVYAYSSLFTAVVFWL
                     ILKWERVADHKGSDKWLILIAYLMGLSIGVHLLNLLAIPAIVLVYYFKNYKVNVKGSM
                     LALLTSVVILGLVLYGLIPGFVVVASKFELLFVNTLGMSFNTGVYIYIIILVAAIAWS
                     LYESYTEKHYLRMTVSYMLAVILAGIPFIGEKLLLGIVIIAALVFFFYQYKKKVNARW
                     LNTSMAMIAVMLIGYSSYSIIMIRSAAQPTMDQSSPDNIFALKSYLNREQYGDRPLLY
                     GAVFSAPLKLDVQGNTCVPIADKTPIYSPKPKTDPAEKDKYIVSGYKSDYEMDSRFMM
                     LFPRMYSSDKSHVSYYTTNYVRNGRKISYDFCGQEKSDVMPTFGQNLRFFFDYQVNFM
                     YWRYFMWNFSGRQNDIQSYGELEHGNWITGIPFIDNILIGDQTNLPTELKENKGHNVY
                     FMLPLILGILGLFFLLNSGKKGNHTFWITGLLFVLTGLAIVVYLNQTPYQPRERDYAY
                     AGSFYAFSIWIGLGVLGVIRLLDKFLPKRASAVIAAVACMGVPVLMAVQNWDDHDRSD
                     RYTARDFGQNYLNSCKPNAIIFTNGDNDTFPLWYNQEVEGVRTDIRVCNLSYLQTDWY
                     IDQMKRASYNSAPLPISWEPKDYISGKNEVLQVEDVLQGQSVDMKTAFDFIKSDDPNT
                     KLEGDGFFPTKQLYLPINADEIVKNGTVAAAKKADIVPQMVFDLPSRVTKSETMMLEM
                     IKENNWKRPIYVAVTVAEEYYPSSLPKYFERTGLVYHLVPVAQKAEGATNVNTDEMYE
                     NMMTKFKYGNIDNPKVYLDDNIMRMCRTHRAMFAYLIEALVNEGDTVRAKKALQYCEK
                     VIPSTTVPHDYSSNMLAHYCYKLGMNKEGNKILDQVARTSVEYLTWYAGLSIAQQNNS
                     SNAIGRNVAILNNVLQISQETKGREIFNKYLPIFETFANKYNTGQ"
     gene            13175..13777
                     /locus_tag="PKH68_02895"
     CDS             13175..13777
                     /locus_tag="PKH68_02895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX11915.1"
                     /translation="MLQVPEILKSVIKNVVWRINPAKKVIYLTFDDGPNSRVTPLVLD
                     ILDRFEVKATFFCVGENVSRFPDVFDEVKRRGHTVGNHTFNHLKGFEYSTDDYVRNVK
                     KASEFIDSRLFRPPHGQIKPSQIKALKDDYLIIMWDFITYDYDKRIEPEKIIAEVKKR
                     SRNGSIVVFHDSLKAEKNVLQVLPEALRFWKENGYEVCHI"
     gene            13970..15241
                     /locus_tag="PKH68_02900"
     CDS             13970..15241
                     /locus_tag="PKH68_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNX11916.1"
                     /translation="MKESVLHHVWQYRLFAANGLTTTGGETVEVIDPGKLNTDAGPDF
                     FNAKLKIDNTLWVGNIEIHNLSSDWVRHNHQRDKAYDNVILHIVSKADAEVRRTNGEF
                     IPQLEMKVPDDIQERYDELISARKWVACEDKLQLLPAVLISSWKSALLTERFERKTAA
                     VESLLSQSQNHWEEAFYISLARSFGFSTNSQAFETMAKSLPLSVLARHKDNVFQLEAL
                     LMGQAGFLEKVEQDEYQLRLHKEYRFLQVKYGLKPMELSQWKLLRLRPDNFPTVRLAQ
                     FASLIFRSSKLFSVILETHSGKELRKLFDTRVTEYWQKHYLFGRKSSVSTKKMGEKSI
                     DILLINTVIPFLFAWGKKKNDQHRMEEAIRLLEEIKPENNAIIHRWKERGVTCQSAFD
                     SQALLQLKKNYCEEKKCLQCRIGHKVLTINK"
     gene            15241..17073
                     /locus_tag="PKH68_02905"
     CDS             15241..17073
                     /locus_tag="PKH68_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX11917.1"
                     /translation="MNKNKRYINRLLLTAIAVVTLHACANRGQGPEGGPKDETPPSVV
                     KSMPSNKALNIKKGKVEITFDENINVLKASENVIISPPQREMPDIKSYGKTVTVQLND
                     TLLPNTTYSINFGDAIVDNNESNPLKNYFFAFSTGSEIDTMQIAGTLVDAKNLNPVQG
                     VTVGIHSDLSDTVFMKKPFLRISRTNDLGRFTVPNVRNGMYRVYALTDGNRDNFYQPG
                     EALAFTPSVFTTSLENYMRSDTIWKDSLTVDTIMTVPAIRYLPDDVVLRYFKDETTRQ
                     YLVKSERTVPHRISMFFNTAADKLPEIKPINVTDWENKILLQKNERLDSLTYWFTDST
                     LIKNDTITLEVKYMKSDSAFQLKPQTDTINFIMKKLKGRAVPKATAKTKKDFLSFNAN
                     LSPQFDVYNPIMLNFEVPLKSIDTTKIHFFQVKDTIQTPLKFRLEKSDSIGMKYVIRH
                     KWIPETNYRLTIDSAALFSIYNQYNDKYKGDMSVKSLDEYSAIKLQLAAFEPTAVLQV
                     LDKNDQVVRTAPAQPKGTLIEYLTPGEYFIRMFLDRNGDGKWTTGSYLLSRQPEEVYY
                     YPKKLKLIKNWEFEETWDYNAIPLLKQKPDELIKKSEGSGSKNN"
     gene            17395..17757
                     /locus_tag="PKH68_02910"
     CDS             17395..17757
                     /locus_tag="PKH68_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11918.1"
                     /translation="MTLWNNDTEIQLFKEALKNFASPEQLFYHLQNGYFAYVPKGSEA
                     EGQTLQSRNSLIGQFTEKWSKTIFEPIAKELGLFAVNSVVCDELGLSRQSSADLALCT
                     TSTVIANVRRKASKYYGK"
     gene            17747..17944
                     /locus_tag="PKH68_02915"
     CDS             17747..17944
                     /locus_tag="PKH68_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11919.1"
                     /translation="MENRKFEIKIYYTGFCTYEIEAQDEAEAIIKARKLSVNQNEIIT
                     NLENWEEADTATDIDNEKSKI"
     gene            17925..18758
                     /locus_tag="PKH68_02920"
     CDS             17925..18758
                     /locus_tag="PKH68_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNX11920.1"
                     /translation="MKKVKSELPLSYVTIKTTKSRIDKGLLAIPVSLIDIFPKKSNKI
                     YLVNERGIVEVKTFTPYNSSSRECRIGGLKTFYENYQVSDGEELVIQLIDNDKYRIIP
                     EKLFQNILSNRLTLLKNSIDDDESDKILYQISEISNLQKIDILKNEFVALSKEKFEDR
                     KKVEKNTPIVRESVPFSIRKILVELYEGKCQVTNFSFFTKKNKPYFELHHIDPEKGNH
                     LKNLLVVSPNTHAQFTHANLIQHFDNEGWLRKVKFNKDEYKVFQIIDDLPNEFEKEIH
                     F"
BASE COUNT         5914 a         3862 c         4108 g         4954 t
ORIGIN      
        1 tttgcggtgg agtgaatgtg gatcgcctca aaaaagtaaa gaaaatgccc atccggatgt
       61 ttcacggagg tgcggatccg gtggtatcac gggagcattc ccgcaatgca tttattgaac
      121 taaaggcaga aggctcaaca cgcgttgaat atgtggagtt tccgggggtg ggtcacgata
      181 gctggacaaa tgctttcgaa tatcccgatt ttttgagctg gatgtattac cagtcccgat
      241 aaaaacatga atatatgtga aaaaataatc attttgatag ttggaaagaa aaaatagata
      301 gtgaaaaaat aaaaaaaaag aagaaattca ccgttagtct tctttatttt ggctattttt
      361 gcacaattaa aaaaatatta aacgaataaa taatggcaaa agcaaaaggt gctgtggtgg
      421 ttaacaccga gcgttgtaaa ggatgtaatc tctgtgtggt atcctgtccg tgggatgttc
      481 tttcgctgaa taaagaagca aattccaagg gatacaatta ttcgtatatg ctggctcccg
      541 aaaattgtgt gggctgtgct acatgtgctt atatttgccc cgatgcgtgc attaccgttt
      601 acaaagtgaa agctgaaaaa acggtttaat tccttacgtc cggctgatta tacctcggtc
      661 gggtcagtaa attggatcat caactaacta agtaataaag ccaagccggg agatgctaat
      721 ttttaacggc tatttcaaaa caatataata tggaagaaat aatattaatg aagggaaacg
      781 aagccattgc ccatgcggct atccgcagcg gatgcgacgg ttacttcggt taccctatta
      841 ctccccagtc ggaagttatg gaaacgctga tggaactcaa accctgggaa actaccggta
      901 tggtggtgct tcaggtagaa agtgaaactg cttccatcaa tatggtatac ggcgccgccg
      961 gttgtggcaa acgagctatg acttcttcgt ccagtcccgg tataagcctt atgcaggagg
     1021 gaatgtcgta tatggcagga gccgaacttc ccgcagtagt catcaatgtc atgcgtggcg
     1081 gtcccggtct cggtactatt cagccaagtc aggccgacta tttccagact gttaaaggtg
     1141 gcggtcacgg cgattataaa gtgatagcac ttgctcccgc atctgtgcag gaaatggcag
     1201 attttacagt gttggctttt gaccttgcat tcaagtatcg cgttacctgt atgttgctgg
     1261 ccgatggagt gattggtcag atgatggaaa aagtacaact gcctccctac cgtccgcgct
     1321 ggactgaaga ggagattcgc cagcaaaatc cctggggcac gatgggtaaa accaacgatc
     1381 gcaaacccaa tgtgatcacc tcactggcac tgcaggccga cgaaatggag gtaatcaacc
     1441 tccgccttca ggcccggtat cgcgaaatag aagaaaacga agttcgtttt gaaaaaataa
     1501 tgtgcgatga tgcagagtac ctggtcgtag cattcggtat cagtgcacgc attggtcaga
     1561 aagcagtgga acttgcccgc caggaaggtt ataaagtagg gttgcttcgt ccgattacgc
     1621 ttttcccatt ccctacggtg gaaatcagca agatggccga tcaggtaaaa ggaatgctca
     1681 ccgtggaact gagtgcaggt cagatggtgg aagatgttcg tctggcagtg aatggccggg
     1741 ttcccgttga attttacgga cgtatgggag gaattgtacc ctcacccgac gaagtgctcc
     1801 gcgcactcaa ggagaaagtc ctggggaaat aaccaatttg cttaaattat taaaaaagga
     1861 aattatgaca acagaagaaa tagttaatcc cgccaatctg gtgtacaaga aaccctcggt
     1921 gctcaatgat gtgaatatgc attattgtcc cggatgcagc cacggcgtgg ttcacaagct
     1981 ggtggccgaa gtgattgacg aaatggatat acaaaaccaa accattgggg tggcacctgt
     2041 aggatgtgcc gtttttgcat acaaatacct cgatgtggat tttcaggaag cagctcacgg
     2101 acgggctccg gctgtggcta ccggtattaa gcgcctgctg cccgaccgct atgtgtttac
     2161 ctatcagggc gatggtgatc ttgctgccat aggtactgca gaaaccatac acgcctgcaa
     2221 ccgcggcgaa aatattgtaa ttatctttat taataatggt atatacggta tgaccggcgg
     2281 gcagatggca cccactacgc ttattgggat gaaaactgct acttcgccgt atggccgtac
     2341 cgccgaactc aacggatatc cactgaatat cacaccacta ctggctcagt tggagggaac
     2401 ctgctatgtg acccgtcaga gtgtgcacaa tgttgccaac atccgcaaga ctaaaaaagc
     2461 cctgcgcaag gcatttgaaa attccatgat gaataaggga acatccgtgg tggaagtggt
     2521 ttcgacctgt aattccggtt ggaaactttc gcccgaagcc tccaacgact ggatggtgga
     2581 aaatatgttc ccggaatatc cattgggaga tatgaaggat aaatagaccc ctaaccccta
     2641 aaggggaatg gacagatatt ttatgtgttt tagcaataaa aaattattta atcattttgt
     2701 tttagctctc cggttccccc tttagggggt tagggggcat ataactaata gcaatgaaag
     2761 aagaaatatt aatctcaggt tttggtggac agggagtgct gtcaatggga aaaattctgg
     2821 tgtattcggg tatgatgcag ggtcaggaaa taagctggct gccatcctac ggtcccgagc
     2881 aacgcggcgg cacagccaac gtaacggtta ttctcagtga cgaacgaatc agttcgccta
     2941 ttctccacaa ctatgatacg gtgattgttc tcaatcaacc ttcactcgat aagttcgaaa
     3001 gtaaggtgaa gcccggcgga accctgattt tcgacagcaa tggatttaag aaatttccaa
     3061 cgcgtaccga tatcaatgtg taccgaattg acgcaacaga gtatgcttat gcgcacgact
     3121 cgtccaagac actgaatatg atcatcctgg gtggttttct gaaaattcgt ccggtggtaa
     3181 aaatagaaaa tgtgctcaag ggactgaaaa aatccctgcc ggagcgccac catcacctca
     3241 ttcctcaaaa cgaagaagcc attaaggtgg gcatgagcct gatacaaaaa tacaactgaa
     3301 aattaattca gaaagataat taaagactta tccgaaaggg ttggtctttt tttttatacc
     3361 taagattatg aattttaata tttttatcct gaaaatcgtc ttaatctaaa aagaatgtta
     3421 tatatttgaa aaaatatttc cccctttatt tgtaatggta aattatccct taatgagata
     3481 acaatcaagt ctacagagta atagttgatg tcatacaatg ttattttccg gttttaacct
     3541 taaattttta agtaaataaa attccgaatt ttaaatcctt aattcataaa accttttaaa
     3601 ttgtaaatgt atgaacttaa ttaatcgtgt aaaaagtatt ttgctaactc ctaaaacgga
     3661 atggccggtc attgaaaccg aaacaactga cgtaaaaacg cttactgtca attatttact
     3721 ccccttggct ttaatcacag ccgtagccac ttttataggt tatggattga ttggtcagaa
     3781 tgtatttggt gtacatatcg gatcggccgg atttggattg cggtatgcgc ttgtcagtct
     3841 ggtaagtact attggcggtg cttttcttac cgcctacatc atcgatttgc tggcacccag
     3901 tttcggttcc gaaaaaaact tcctccaatc attcaagttg gtggtttatt cctacacacc
     3961 catgctggtt gcgggtattc tgctaattat tccatcgctg ggtattctgg caacgctggc
     4021 cggcttgtat ggcttatacc tgctctacat cggactgcaa cccatgaaaa aaacgcccga
     4081 agacaaaaaa acaagctatt ttgtagtcag cctgatagtt gtaatactcg gttctgtagt
     4141 cctttcagct attttatctt ccatcttcat cacatccgga attcgctatt gattttttga
     4201 ataaattctg ttttttaata aaagcaaaat cagtagaata ttattattta attcaaaaaa
     4261 tccaaggata tgaaacgaat tgtatttttt ctttgtgtca gttttctgtt tagtttacaa
     4321 ctgagtgccc agggctggct tgaacgtgcc ggtcagcggg ttaaagaccg agtggtggaa
     4381 aaagtggaaa accgtattga caaaaaaacc gatgaagcaa ccgataaagt gctcgataaa
     4441 gccgaaggat cggtaaaaaa atcgggaaag aaaaataaac aaggtcaaca agatgcagaa
     4501 gaagctgctg aaaccgatga aaaccaggat gaaaatgctg ctcccaaagg taaaactgct
     4561 cagaaactcg aatcggtcag tgcctacgat tttgttccgg gtgataaaat cctctttttc
     4621 gaagatttca gccaggatgc catcggcgat tttccggcac tatggaccac caacggcagc
     4681 ggtgaagtga agactgtcaa cattgctccc ggcaaatggt ttcacatgaa cggtgatgat
     4741 gctgtatatt gttataccaa agagattaat tttcccgata attttattgt tgaatttgac
     4801 atcattcccg agaaaaattt tcagtatgga atatgcttaa tcttgtatca ggaggatccc
     4861 gaaaacccca aagaagtaaa tgatgatctt tttccgggat taagaggatt gcgtattgcg
     4921 ccaaacagcg atggttggga aacaaatggt tatgaatcag acccaaacaa accctggctg
     4981 gatggcaaag gatccaaaaa tcctcttatt gcagaagaac tgaatcatgt gattctatgg
     5041 attcaaaaac gcagggtgag gatttatcat cagggacaaa aagtgcttga tatgcccaca
     5101 aacatctatg cagataccaa atttaaccgg ttgcttttct caggctggga tcgtaactgc
     5161 gcacctttgg ttagcaatat caagattacc accgcatcgc ccgacacccg cagcaagctc
     5221 attaccgaag gtaaactcat cacttacggc atcacttttg atgtgaacaa agccgatgtg
     5281 aagcctgaat cctacggcac tctcaagagt atagccgatg tgttgaccga aaatccgggt
     5341 gtgaaagtga aaattaccgg acacaccgac agtgatggtg atgatgccaa aaatctcgaa
     5401 ctgtccaaac gaagagccga atcggtaaaa aacgaactgg tgaaaaactt tggcattgat
     5461 gcctcaagac tttctaccga tggagctggt gaaacaaaac ccattgctgc aaatgataca
     5521 ccggcaaata aggcaaagaa ccgcagggtg gagtttataa agttataaat gaatgcagac
     5581 agatatccgc cggttcggag gatatctgtc tgccatttta catcccaaaa acttaaaaaa
     5641 acctctatta acctatgaaa aaatcagttg taattatttt gttcctttca acagcggtta
     5701 caagtcttgt taccgctcaa aaaaccgcat tggagtattt gaaaactgtg cccggtaacc
     5761 cggattctgt ttgtgtctgt ctgaaagaag tgagacagaa ttatgaaaca gcagtaagtg
     5821 aactctacga cattgttaaa gaggatattc aaagccgaac aagagccgtt gaagactaca
     5881 tggatcaaaa tcaggacaga atgaaagctt ccatgatgaa agaaatgtct gaaaaaacgg
     5941 ggatgtcgca acaggaactg gaaacgctct ccaaaaagaa aaacctgtcc aaagaagaca
     6001 aacaggcact gatggataaa gcgctcggac agcacaacct ttcgatgggc gaggtgcaga
     6061 acatgaaaaa catggatgca aatgcccgaa aagcatgggc agaggcatat gcaaccgaac
     6121 aaatggcgat ggctcaggga aatagtaaaa atccacaagt aaaggtcaat aacgataaag
     6181 cgatgggtca ggtagcgctc atgcaggagc agcaaacggt cagaaatcaa ctcagcactt
     6241 tcgaaactga aacagctaac cgctacaaaa ccctgataca aacttcaggt aaagaaaaaa
     6301 tggcagctga tcttcagaaa cttgaaaacg aacttcgcac cattggcagc atctacgaat
     6361 gcaatgaatg tccgcctcca ccggctgagg atgtaaaaca ctggaatgcc gtgatgaaga
     6421 aaattcacga taccaaaatt cagtattgcc ggctcaacac gccaaaaatg atagcctact
     6481 tgaactggta tcgcgaaaat ctggtgaaaa atattccgct ttacgacaaa tcggaagaac
     6541 ttcagtataa attgacttcg gctacaacca acacgcagct tacaccgccc gataaaggca
     6601 tcttttcact gcaggctgta caaggcttta tcggagcaaa gcagaaatta tttgaattta
     6661 aggactacga aatcgaaaat ttcacgtatg attttatcaa ataatcggtt tgttttcagt
     6721 aaaaaaaagt gattgcctaa ccaaaaaata ttccaatgaa atcatacctc acaatatttt
     6781 ttctgttgat ttcattatcg ctggctgccc agagtaccaa cgcggacgaa atacgccgcc
     6841 aaatggccaa agtgcgtcag agcaccaact ggagcgaccc ggcagcagcc agaaaagcca
     6901 ctgaagaaat ccgccgcctt gctgccaaat tgtccgggaa ccaaaatctg ccagttattg
     6961 gtggctcgga taattcgaca ccgctcggta agcaaaccga agtgaaatca tctgtcaatc
     7021 ccacgaaaga aaatattctg agaatagccg aacgctttta caaccgaagt tacaagcagt
     7081 tgaatgccat tgaaaagagc cgtttcgacc tcgaatatca aaatgcgcaa aaaaacaagt
     7141 tcaaccgtca ggctgtaaat gcgctgacaa gcaacggagc caacctgatg atgtttggca
     7201 ccaatcacca cgaaggctgc gtatatctta cctcggccgt gaaagctgat attaatgata
     7261 ccctggctgt gaataatttt ggagcttacc tgcgcatcat cgactcgctg aaagtctcgc
     7321 tgccggtttt gctgtatgcc aataccctgt ttgataaaag tccggttatc ctcacccaaa
     7381 tcggatgcag ttattttgaa ctgaatgact tcaaaaaagg ggaaacctac ctgaaactgg
     7441 cgttgcaggt caatcccggt ttcggcaagg ctcattcggc attgtgcgaa ctttatctga
     7501 aaaccggccg ctggaaagag gccttgcagg aactgatagc cggagtggtc aatcagggct
     7561 gctcgtacat gcaggcgcat gccaactttc agcagctggc agatgtgtct tcggccaata
     7621 aaagtggcgg ctcagcaaaa gcgcagggat tctacaatca gaataattta tcagacgacg
     7681 atgcgctggc gccgctggtg ccggaaaata aagggaaagt gcagattgat ccgaaagaca
     7741 tgctcgcctc attagtgccc gaagacaata tccccgatga agataagctc gcgcctttag
     7801 ttcctcagga caataaaatt aaaatgcccc agttcgccaa catcaccagc gtcaacgact
     7861 ggatggaagg gggcggatat tcgggagcta tcagcgcatt tcagagtttt ctgacaaccg
     7921 acatggctgc agccaacgaa ttgcagcaag tcctaaaaga acagccaaat atttcgcctg
     7981 atgccgttct gcgcgattat cccaacgaac gttttgccat cgattgcatc acagaaatga
     8041 taggcgatat gtccaacgac gaaagtatga agttgtcaaa aaaaacggat aaatacattg
     8101 aacaggctgt aagctatcaa caaatataca tggataaata tcagcgtatt tccaaggatt
     8161 acatgagttg cctgcaggct tgtccgaccg aaaatccgat gcacgattat tgtaccggtg
     8221 aatgtcagcg gaaatactgc ctggagatgt gcaacgctgc caattcgtac aatcaaaacc
     8281 tgcagaatat tttcgacgac tggacccgcg atttcaacga aagtaaacaa aaacaacaag
     8341 aactgcttga cgacttgtac ggatttacca caccctggtt taagaaactc tacagccctt
     8401 actggagccg aattgaagcg tatgaaatcc gccgggtggc tttactcatc gtaggcaata
     8461 ccgttacagg atatgctcat cgtgcattga cttttcccgc tcataccgac tgtggcagtg
     8521 attgttccgt atttgtaaat gccgccccac caccggtaga ggaggtggat aagaaagaac
     8581 ccaacggcaa tgaatgtccg tttgagggaa ataaattatc acttggtatt ggcttgtgca
     8641 gtgtcgattt cgattgtgaa agcatcgaag caggctgttc ggcaggagtt tccatttcgg
     8701 ccaaacgaaa tttcaaaaat aaaagttcca ccctttttgt aggtgcagga ggcgaagcca
     8761 atctgggagc cgtatccggc gaaatcaaaa ccggggtgac ttttacaaaa tatgacaacg
     8821 gcaatatgga tgtaggcgga aaatttgaaa tgaccggaaa agtaggtcct gtgggtaaaa
     8881 attacgaaat gtccgctacg ctcatggaag gagccaatgt agagggcaaa aatgtgatag
     8941 gattataagt tacacactcc gggagtttta taaaaaaaaa tatcatcaaa ataaaacgat
     9001 tattcagaaa aatgtattca tctaaaacca tcgaaaaaat gaaaaattca atttttattc
     9061 tcagcatcgt ctttgtactt gcagcctgcg ggcagaagcc ggccgataaa gcagcaaccg
     9121 acgaaaaaag tgccaccgaa acggttacac aggtaaagaa aggaaaaaaa tacccgattg
     9181 aaaaaggtat tgtctatcag aaatcagatg tcatgggcat cgtaagcaat cccgtaattt
     9241 attttgataa atggggcgaa tgggaagcca ccgaaactac aacttccatc gaaatgatgg
     9301 gcataaaaga atcgtccacc tcgctcaaaa tagtgaaggg ggaagataac tgggaaattg
     9361 atatggataa aaagaccggg tttcactata atcgcgctgt aatgattaat cagatgggtg
     9421 tggatatgga aaatctaacc accgaaatca tgggcaaaat gaagctggag aaagtgggtg
     9481 aagaaaagta tttgggctat ccgtgtgtaa aatacaaaat gaagaacgat gaaatgaaaa
     9541 tggatgtgga atatctcatg tgtggtaatc tgatgatgaa gatgaaaggc aaagccatgg
     9601 aaatacctac tgaattagaa gtgacaaaaa ttgaagcggt aacaccacct gccgaaaaat
     9661 ttgaaattcc tgccggggtg aaaattacgg agcaatagtt ttaatccctt atcggttttt
     9721 tttataaatc ggtgacagcc gctatcgtct gatagtggct gtcgcttttt tttaatattc
     9781 cctaaaatgc agcccgctat acctgcaaac tgaattttct ttgtatcttt gaaagttttt
     9841 ttacgaaaag tgtaaattaa ataatacttt caatactaat ttcaaaatca atgaaaaatt
     9901 tcaggttatt gaacaatatc tttgggtggg tagcttttgc ggtggcagcg gttacttatc
     9961 tgcttaccat tgagccgtcg gccagtttct gggattgcgg tgagtttatc tcttcggctt
    10021 ataagctcga tgtgggtcac cctcccggag caccattctt tatgctcaca gccaaatttt
    10081 tcaccctttt tgcctccgat cccacacagg ttgccatgat ggtgaattca ttttcggcca
    10141 tcgccagtgc atttaccatt ttattcctct tctggactat cacccacctg gctcaacaag
    10201 tactgggtaa atccgaggaa gaatattctg cagcaaacat cataggcatt cttgctgccg
    10261 gcatggtagg agctctggca tacaccttct ccgacacatt ctggttttcg gctgtagaag
    10321 gggaagtgta cgcttactca tcgctgttca cagcggtggt tttctggctt attctcaagt
    10381 gggaacgcgt agccgatcac aaaggttccg acaaatggct cattctgata gcctacctga
    10441 tgggactttc catcggtgtt cacctgctca acctgctggc cattccggct atcgtgctgg
    10501 tgtattattt caaaaattac aaagtaaatg tgaaaggttc catgctggca ttactcactt
    10561 cggtggttat cctgggactg gtactttacg gattgattcc cggttttgtg gtcgtagcat
    10621 ctaaatttga acttcttttt gttaacacac tgggtatgtc attcaatacc ggtgtatata
    10681 tctacatcat tattttggtg gcagctattg cctggtcgct ctacgaatca tacaccgaaa
    10741 aacattacct gcgaatgaca gtttcgtaca tgctggctgt gattttggcc ggcattccct
    10801 ttatcggcga aaaactgctg ctgggtatcg ttataattgc cgcattggta tttttctttt
    10861 atcaatacaa gaaaaaagtg aatgcccgct ggctgaatac gagtatggcc atgatagccg
    10921 tgatgcttat tggctattcg tcgtattcca tcatcatgat ccgttcggcc gcacagccta
    10981 ccatggacca gagttcaccc gacaatattt ttgccttgaa atcatacctc aaccgtgaac
    11041 agtacggtga tcgtccgttg ctatacggag cagttttcag tgctccgctg aaactcgatg
    11101 tgcagggaaa tacctgcgtg cccattgctg ataaaacgcc catttattcg ccaaaaccca
    11161 aaactgatcc tgctgaaaag gacaaataca ttgtttcggg atacaagagt gattatgaaa
    11221 tggacagtcg gtttatgatg cttttccccc gtatgtacag cagcgacaaa tcgcacgtat
    11281 cttattacac taccaactac gtgaggaacg gccgtaaaat atcctatgat ttctgtgggc
    11341 aggaaaaaag tgatgtgatg cccacctttg gtcaaaatct tcgctttttc ttcgattatc
    11401 aggtcaactt catgtactgg cgctatttca tgtggaattt ctccggtcga cagaacgata
    11461 ttcagagcta cggtgaactg gaacatggca actggattac cggtataccc tttattgata
    11521 atattttgat tggcgatcaa accaatctgc caaccgaact gaaagaaaat aaaggtcata
    11581 acgtgtactt catgcttccg ttgatactgg gtattctggg gcttttcttc ctgctcaatt
    11641 ccggcaaaaa aggcaatcac accttctgga ttaccggtct gctatttgtg cttaccggcc
    11701 tggccattgt ggtatacctg aaccaaacac cgtatcaacc ccgtgaacgt gattatgcct
    11761 atgccggatc tttctatgct ttcagcatct ggattgggct tggagtactc ggtgtaatcc
    11821 gtttactcga taaatttcta cccaaaagag caagtgctgt tattgctgca gtagcctgta
    11881 tgggtgtgcc ggtgttgatg gccgttcaaa actgggatga tcatgaccgc agcgaccgct
    11941 atacagcacg tgatttcggt cagaattatc tcaattcctg caaaccaaat gctattattt
    12001 tcaccaacgg tgataacgat actttcccgc tctggtacaa tcaggaagtg gaaggcgttc
    12061 gaaccgacat tcgggtgtgc aacctgagtt atttgcaaac cgactggtat attgaccaga
    12121 tgaagcgggc atcatacaac tctgcgccgc tgcccatttc gtgggaaccc aaagactata
    12181 tttcgggtaa aaatgaagtg ttgcaggtgg aagatgtgct gcagggacaa tcggtagata
    12241 tgaaaaccgc tttcgacttt atcaaaagcg acgaccccaa tacaaaactg gaaggggatg
    12301 gatttttccc aaccaaacaa ctttatttgc ccattaatgc cgacgagata gtgaaaaacg
    12361 gaacggtagc cgctgccaaa aaagccgata ttgttccgca aatggtcttc gatttaccct
    12421 cacgtgtgac gaagagtgag accatgatgc tcgaaatgat taaggaaaat aactggaaac
    12481 gacccattta tgtggctgtg acagtggccg aagaatatta cccatcatca ctacccaagt
    12541 attttgaacg taccgggctg gtgtatcatc ttgtacccgt ggctcagaag gccgaaggtg
    12601 ctaccaatgt gaataccgac gaaatgtacg aaaatatgat gacgaaattc aaatacggca
    12661 acattgacaa tccaaaggta tatcttgacg acaacatcat gcgtatgtgc cgcactcacc
    12721 gggctatgtt tgcttacctc atcgaagcgt tggtcaacga aggcgatacc gttcgtgcta
    12781 aaaaggcact gcagtattgc gaaaaggtga taccatccac aactgttcca cacgattact
    12841 cctccaatat gcttgctcat tactgttata agttgggcat gaacaaagaa ggtaacaaaa
    12901 ttctggatca ggtggcacgc acaagcgtag aataccttac ctggtatgcc ggtctgagta
    12961 ttgctcagca aaataattcg agcaatgcca tcgggcgtaa tgtagccata ttaaacaacg
    13021 tgctgcaaat cagtcaggaa accaaaggaa gggaaatatt taacaaatat cttcccatct
    13081 ttgaaacatt tgccaataaa tataatacag gtcaataaaa aaaatccggt gtctgcaaat
    13141 aattttcagc caccggattt tattggaata atcgatgctt caggttcccg aaatactaaa
    13201 atcggtcatt aaaaatgttg tctggcggat aaatcctgcg aaaaaagtca tttacctcac
    13261 ttttgacgac ggaccgaatt cacgggtaac tcctctggtg ctcgatatac tcgaccggtt
    13321 tgaggttaag gctacctttt tctgtgtggg tgaaaatgtt tcccgctttc cggatgtgtt
    13381 tgatgaagtg aaacgacggg gacataccgt tggaaatcac acatttaatc atctgaaagg
    13441 ctttgaatat tcaaccgatg actatgtgag gaatgtaaag aaagcttcgg aatttataga
    13501 tagccggcta ttccggccgc cacacggaca gattaaacca tcgcaaatca aggcattgaa
    13561 ggacgattac ctcataatca tgtgggattt tatcacctac gattatgata agcgtattga
    13621 acccgaaaaa ataattgcag aggtaaaaaa gcgcagtcgg aatggttcaa ttgttgtttt
    13681 tcacgattcg ttgaaagctg aaaaaaatgt attacaggtt ttgcccgaag cattgagatt
    13741 ttggaaggaa aacggatacg aggtttgtca catataacac ctctaattca aaaaaggaca
    13801 ttttagaata tttgaatata atttctttta atcttttgtt attcagttaa ttgtgataac
    13861 aaaggtgttt gatttttata aacagcatta ttactcaccc atattccccc tttcccgata
    13921 tcccgaagct tcgggacggg acacagtagg gggttagggg gcttttaaaa tgaaagaatc
    13981 agttcttcac catgtatggc aataccggtt gttcgcagct aacgggctga ctactaccgg
    14041 tggcgaaacc gtggaggtga ttgatccggg aaaattgaat acggatgcag gacccgattt
    14101 tttcaatgca aaactgaaaa ttgacaatac actctgggtc ggaaacattg aaattcacaa
    14161 cctgagttcc gattgggtga gacacaatca ccaacgagat aaagcatacg acaacgtaat
    14221 actgcacatt gttagtaaag cggatgctga agtgagaagg acaaatggtg aatttatccc
    14281 tcaactggaa atgaaagtgc ccgatgatat tcaggaacga tacgatgagc tgatttctgc
    14341 ccgcaagtgg gttgcctgtg aagataaatt acagttgcta cctgctgtac tcatcagcag
    14401 ctggaaatcg gccttactca ccgaacggtt cgaacgtaaa acagctgctg tcgaatcgct
    14461 gctttcgcag tcgcaaaacc actgggagga ggctttttat atttctttgg cacgtagttt
    14521 tggattttcg accaacagtc aggctttcga aacgatggcc aaatcattac cactgtctgt
    14581 tttggcaagg cataaggata atgtctttca actcgaagcg ttgctgatgg ggcaggccgg
    14641 ttttctcgaa aaggtggaac aggatgagta tcaactccgt ttacacaagg aatatcgttt
    14701 tcttcaagtg aagtatggac tgaaaccgat ggaactgtcg caatggaaac tgctcagact
    14761 tcgtcctgat aattttccta cggtaaggct ggcgcagttt gcttcgctaa tattcaggtc
    14821 ttcaaagtta ttttcggtca tactcgaaac acattccggt aaagagttgc gaaaactctt
    14881 tgataccagg gtaacggaat actggcagaa acattatctt ttcggaagaa aaagttctgt
    14941 ttcgacaaaa aaaatgggcg aaaaatccat tgatattctg ctgataaata ccgtgatacc
    15001 ttttctgttt gcctggggca aaaagaaaaa tgaccaacac cgaatggaag aagccatccg
    15061 gttgttggag gaaatcaaac ccgaaaacaa cgcgatcata caccggtgga aggagcgggg
    15121 agtaacctgt cagtcggctt tcgactcaca ggcattgctg caactgaaga aaaactactg
    15181 cgaagaaaaa aaatgcctcc aatgccgcat tggacacaaa gttctgacaa ttaacaagta
    15241 atgaataaaa ataaaagata cataaatcgc cttctgctga cagcaattgc tgtagttaca
    15301 ctgcatgcct gtgctaaccg cggacaggga cccgaaggcg gcccaaagga tgaaacaccg
    15361 cccagtgtgg tgaagtctat gccctctaac aaagcattga atattaagaa aggcaaagtg
    15421 gaaatcactt tcgatgaaaa tattaatgta ctgaaagctt ctgagaatgt gattatctca
    15481 cccccacagc gcgagatgcc cgatatcaaa tcgtatggca aaacagtgac tgtgcaactc
    15541 aatgatacat tactccccaa tacgacgtat tctatcaatt ttggcgatgc cattgtggac
    15601 aataatgaaa gcaatccgct gaaaaattac tttttcgctt tttctaccgg cagcgaaatt
    15661 gataccatgc agatagccgg gactctcgtc gatgccaaaa atctgaaccc cgtacagggt
    15721 gttactgttg gtattcacag cgatttgtcg gatacggtat ttatgaaaaa accgttttta
    15781 cgtatttccc gcaccaatga ccttggccgt tttacggtac ccaatgtgcg gaatggaatg
    15841 taccgggtgt atgcactcac cgatggcaac cgcgacaatt tctaccagcc gggtgaagcg
    15901 cttgcattta ctccttcggt atttaccacc tccctcgaaa attacatgcg atcggatact
    15961 atctggaaag acagcctcac agtggatacc atcatgaccg ttccggctat cagatacctg
    16021 cccgatgatg tggtgttgcg ctattttaag gacgaaacca cccggcagta tctggtaaaa
    16081 tctgaacgta cagtgccaca tcgtatatcc atgtttttta atactgcagc cgataaattg
    16141 ccggaaataa aaccaataaa cgtaactgat tgggaaaata aaattttact tcaaaaaaac
    16201 gaacggcttg actcgctaac ctactggttt accgattcta cactgataaa aaatgatact
    16261 atcaccctgg aagtgaaata catgaagtcg gattcggcct ttcaactcaa gccgcagacc
    16321 gataccatca attttatcat gaaaaaattg aaaggacgag ctgttcccaa agccaccgct
    16381 aaaaccaaaa aagattttct cagtttcaat gccaatctat cgcctcagtt cgatgtgtac
    16441 aaccccatca tgctcaactt tgaggtgccg ctgaaaagca ttgacaccac caaaatccat
    16501 ttctttcagg tgaaggatac gatacagacg cctttgaaat tccggctcga aaagagcgac
    16561 tccattggga tgaaatatgt gatcaggcat aaatggatac ccgaaacaaa ctaccgcctc
    16621 acgattgact cggctgccct gttcagcatt tacaaccagt ataacgataa atacaaaggt
    16681 gatatgagtg tgaaatcgct ggatgaatac tcagccatta agcttcaact ggctgctttt
    16741 gaaccaactg ccgtgttgca ggtgctggat aaaaacgatc aggtggtacg caccgctccg
    16801 gcacaaccca agggaacttt gatagaatac ctcacgcccg gtgaatactt tatccgtatg
    16861 tttcttgacc ggaatggaga tggaaaatgg actaccggca gctatctgct tagccgtcag
    16921 cccgaagaag tgtattacta cccgaaaaaa ttaaagttga taaaaaactg ggaattcgaa
    16981 gaaacttggg attacaatgc aataccgtta ttgaaacaga agccggatga actgattaaa
    17041 aaatccgaag gctcaggttc gaaaaataat taatcggagc gaacagcgtt tattgaaatt
    17101 ttgtactttt gctttgaaaa gaatgaaata cagaactgtc ttaaagtccc ctttgagcct
    17161 gtccctgcct gtcccgaatt ttttcgggaa acttgtttcg ggaggtttta ggggcaattt
    17221 agttgtttgc ggcaatagtt cagttggcag agcgctccga tttcaatcgg agaggtggcg
    17281 ttaagttagg ttttattaag ttgtatgcgg aaatagttca gatgcggtta cggcataaaa
    17341 aagacagacc gaaaaaaccg tgaaaatgac tatctttgta aaaaagaaaa taatatgaca
    17401 ctttggaata atgatactga aattcaatta tttaaagaag cattaaaaaa ttttgcttca
    17461 cctgaacaac ttttctacca tcttcaaaac ggatactttg catatgtccc aaaaggctca
    17521 gaagcagaag gtcaaacttt acaaagtaga aactcactta taggtcaatt taccgaaaaa
    17581 tggagtaaaa ctatttttga accaatcgca aaagaacttg gactttttgc tgtcaatagt
    17641 gtagtatgcg atgaacttgg cttatcgaga caatcaagcg ctgaccttgc tctctgtaca
    17701 acttccactg ttatcgctaa cgtaagaaga aaagcttcaa aatactatgg aaaatagaaa
    17761 atttgaaatt aaaatttatt atacggggtt ttgtacttat gaaattgaag cacaagatga
    17821 agcagaagca attataaaag caagaaaact ttcagttaac caaaatgaaa ttataaccaa
    17881 tttggaaaat tgggaagagg cagatacagc aacagatatt gacaatgaaa aaagtaaaat
    17941 ctgaattacc acttagttat gtaactatta agacaacaaa aagtcggata gataaaggct
    18001 tattggctat ccctgtttct ttgattgaca tttttcctaa aaaaagtaat aagatatatt
    18061 tagttaatga aagaggaata gtagaagtta aaacatttac accatacaat agcagtagtc
    18121 gtgaatgtag aattggcgga ttaaaaacat tttacgaaaa ctatcaagtc tcagatggtg
    18181 aagaattagt tattcagtta attgataatg ataaatatag aataattcca gaaaaattat
    18241 ttcagaatat tctgagtaat cgtttaactc tacttaaaaa ttcaattgat gatgatgaat
    18301 ccgacaagat tttatatcaa atttcagaaa tctcaaatct tcaaaagatt gacattctaa
    18361 aaaatgaatt tgtagccctt tctaaagaaa aatttgaaga cagaaaaaaa gtagaaaaaa
    18421 atacgcctat tgttagagaa agtgttccat tctcaattcg taaaatttta gtagaattgt
    18481 atgaaggtaa atgtcaagtt actaattttt catttttcac aaaaaaaaat aagccatatt
    18541 ttgaattgca ccatattgac ccagaaaaag gtaaccacct aaaaaattta ctggtagtta
    18601 gtccgaatac tcacgcacag tttacacacg caaatcttat acagcatttt gataatgaag
    18661 ggtggctaag aaaagtaaag tttaataaag atgaatataa agtttttcaa attattgatg
    18721 atttgcctaa tgaatttgaa aaagaaattc atttttagca tcctgtacca tttcccaaag
    18781 aattacctaa tagactaatt caactttatt cttttaaagg cgatataatt ttagaccc
//
LOCUS       DAOPZK010000027        18739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3088_length_18739_cov_22.344948, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000027 DAOPZK010000000
VERSION     DAOPZK010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3088_length_18739_cov_22.344948"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="PKH68_02925"
     CDS             complement(<1..1736)
                     /locus_tag="PKH68_02925"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNX11921.1"
                     /translation="MKKISLFLIALLLTISVKADEGMWMLPLIEKLNIQKMQGMGLTM
                     TAEEIYSDKNVSLKDAIVIFGGGCTGVMVSPEGLVFTNHHCGYDAIQKHSSVEHNYLK
                     DGFTANELKDEIPSPGLAVTFLVKIQDVTDRVLPQIENLDGKKRDAVQDSVCKAISDE
                     AAKGTHYTAQVKSFYSGNEFYLFVYERFNDVRFAYAPPSSIGKFGGETDNWMYPRHTG
                     DFSVFRVYCAPDGKPAEYSKDNVPYSPKRFVTISNQGYQDGDYAMILGNPGSTERYLS
                     SWGINERVNNGNKARIEIRGAKQDVWKKYMLANEAINIAYAGKYARSSNYWKNSIGMN
                     DAIEHLKVVDQKQKQEAEFAAWVKADAQRVSKYGNVLKDLEDNYNAIYPYSHALNYLR
                     ESLLSGVELPRIASMVKRGLKNNETSDKILQRAEELYKDYYPEVDQATFSVLLDAYRK
                     SVPADALPEIYNTIDKKFKGNYAKYAAYLFEKSAFVSPARLSNALNKKGYNFEKDPAI
                     VFYNDVLKTMNTFNNQKYADALQKINTAERLYEAGLKEIAAQNNTARYPNANFTMRLT
                     YGKVGSYNPADAT"
     gene            complement(1946..2965)
                     /locus_tag="PKH68_02930"
     CDS             complement(1946..2965)
                     /locus_tag="PKH68_02930"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNX11922.1"
                     /translation="MENIDWSNLGFGYMKTDYNVRCTYKDGKWGEIRTCEDENITMHM
                     AATCLHYGQEAFEGLKAFKGKDGRIRIFRLDENAKRLQSSCEGIMMAKLPVEMFEEAV
                     LKVVKLNERFVPPYGSGASLYIRPLLIGTSAQVGVKPAKEYEFLIFVTPVGPYFKGGF
                     ATTPYVIMREYDRAAPLGTGTFKVGGNYAASMVAGMKAHDMGYSAVFYLDAKEKKYID
                     ECGAANFFGIKNDTYITPKSTSILPSITNKSLMVLAEELGMKVERRAVPEEELATFEE
                     AGACGTAAVISPIERIDDLQENKTYIISKDGKPGKWSEKLYHKLRAIQYGDEPDTHGW
                     ITYVD"
     assembly_gap    3172..3271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3361..4002)
                     /locus_tag="PKH68_02935"
     CDS             complement(3361..4002)
                     /locus_tag="PKH68_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNX11923.1"
                     /translation="MSRSIGKLDSGELGSRAMLSTAYLPPVSYFMCMLHANEIWLEAC
                     ENYVKQSYRNRCRIATANGVETLSIPVESNSGQKNPIRDVRISSHGNWQQTHWRAITS
                     AYMNSPFFEYYQDDFRPFYENKWTFLWDYNLELLTLILKLLNENPKIIPTETYQNNLF
                     DFNDLREIIHPKKEPVLVTKPYYQVFETKYGFLPDLSIIDLLFNMGNEAVMIL"
     gene            complement(4115..5521)
                     /locus_tag="PKH68_02940"
     CDS             complement(4115..5521)
                     /locus_tag="PKH68_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNX11924.1"
                     /translation="MNTAITASRFKKPAKQWIKCAFWSLIFVLFVSWVGNFWWLLLLP
                     FIFDVYITKIIPWTWWKKSKNKTVLAIMSWVDAIVFALVAVYFIHTYLFQNYQIPTSS
                     LEKTLLVGDFLAVSKASYGPRVPMTPLSFPLVQHTFPILNSKSFLEKPQWEYKRLKGT
                     DKLERNDIVVFNFPTGDTVCVKQQNPDYYTLCFLYGRDVVRSRTDIFGEIVYRPVDRR
                     ENYVKRCVGVAGDWLEVKDNQVIVNGKPQEHFPGIQYNYFVQTDGTRLTNEVLEDMNI
                     SNEDIVLLDNSQAAGLITSLGLNPNLPVYNFPLTAESYSKMQNTRGVVKLVVEPSGIL
                     MEDHTFPLGNTYNWTRDNYGPIYIPKAGAVLKLTLANLPIYERIIRVYEKNKLEVRDG
                     QIFINDQPTTSYRFKMNYYWMMGDNRHKSADSRYWGFVPEDHIVGRPMFVWLSLNKDK
                     SLFNGKIRFDRFFKNAER"
     gene            complement(5570..6286)
                     /gene="dapB"
                     /locus_tag="PKH68_02945"
     CDS             complement(5570..6286)
                     /gene="dapB"
                     /locus_tag="PKH68_02945"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNX11925.1"
                     /translation="MKLALIGYGKMGKIIERIALERGHEIVSIIDIDNRDDFKSDAFK
                     SADVAIEFTVPQVALDNYKQAFEAGVAVVSGTTGWADQQEKVKADVLKNGYTLFWSSN
                     FSLGVNIFMAVNKYLAKIMNRFPEYNVEMTEVHHIQKLDAPSGTAITLAEGILDNIDR
                     KTGWVKEIQQNDNELAIKSIREGQVPGIHTIRYESFADIIEITHDAKSREGFALGAVL
                     AAEFTCGKKGFLGMNDMLNF"
     gene            complement(6450..6806)
                     /locus_tag="PKH68_02950"
     CDS             complement(6450..6806)
                     /locus_tag="PKH68_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNX11926.1"
                     /translation="MKTLIIGASKNPERYSYKAAERLLHHNHEIELLGLRPDVIFDRE
                     IDTEKKNYESIDTVTLYVGPQRQPEYYDYILSLKPRRVIFNPGTENEEFYKILKDNNI
                     EAEVACTLVLLGTGQY"
     gene            complement(6956..8002)
                     /gene="aroB"
                     /locus_tag="PKH68_02955"
     CDS             complement(6956..8002)
                     /gene="aroB"
                     /locus_tag="PKH68_02955"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNX11927.1"
                     /translation="MTESKTIITSDFTAELNQLLNEWQGNKIFILTDEHTEKFCLPEV
                     MKCSMAEGSHIITIEHGDNHKNIDSAVKIWTLLTENGATRKSLMINLGGGMITDIGGF
                     TASTFKRGLSYINIPTTLLGAVDAATGGKTGINSRGLKNEIGVFSPASTVLVNVDFFR
                     TLDARNFLSGFAEMVKHALIDSRRAWNEVLAFDFDNIDFENLKELLAYNIGIKQRIVA
                     EDPTERGIRKALNLGHTFGHAFETQSYKTAQPLLHGYAVAYGMICELYLSHIKLGFPK
                     EDLQKYRHFVQENYGRFNCDCSNYDTILELMMHDKKNDNQEINFTLLSDVGEIRINQT
                     ATKEEIYECLDILF"
     gene            8183..8476
                     /locus_tag="PKH68_02960"
     CDS             8183..8476
                     /locus_tag="PKH68_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX11928.1"
                     /translation="MLPDNTEKMEEAAYMLRAISNGTRLQVIATLSEKEELSVSQLQE
                     QLNCEQSLLSHHLTDMRAKGVLNCRREGKNCYYSLKNKQIIQILNCINSCNCN"
     gene            8510..8710
                     /locus_tag="PKH68_02965"
     CDS             8510..8710
                     /locus_tag="PKH68_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNX11929.1"
                     /translation="MMIAFLKKNILPIIGLLVGAAGGYLYYYYVGCASGTCPLTSNPY
                     ISVLYGALMGFLLFDIFKKKEK"
     gene            8840..9091
                     /locus_tag="PKH68_02970"
     CDS             8840..9091
                     /locus_tag="PKH68_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11930.1"
                     /translation="MKQLKDYFRMWDFARWFKLTLAVALGLAYVFGSGSIYLFGSLFF
                     GVQAILNMGCGCATGNCTTTVKKDEKQISYDIEEYKPTK"
     gene            9093..9326
                     /locus_tag="PKH68_02975"
     CDS             9093..9326
                     /locus_tag="PKH68_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNX11931.1"
                     /translation="MYSTFLVNGEACLTCNNQVLKKIGTLQGVFGAEIDRIRGEVVVS
                     HTDEVSREMIEKTLNELGFFEENNSDNNCEINT"
     gene            9340..9855
                     /gene="trxA"
                     /locus_tag="PKH68_02980"
     CDS             9340..9855
                     /gene="trxA"
                     /locus_tag="PKH68_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX11932.1"
                     /translation="MKKLALATTLLISLAIVSCAGTSKDKEKKSGTTVQTETKKVENN
                     KTETKKMGTIHLTKEEFLKKVVNYETNSKEWKYLGDKPCLIDFYADWCGPCKVVAPIL
                     EDLATEYAGQIYIYKINTDEEQELAAAFGIRSIPSLLFVPMGETPQMTQGAMPKANLK
                     EAIDNVLLKKK"
     gene            10061..10453
                     /locus_tag="PKH68_02985"
     CDS             10061..10453
                     /locus_tag="PKH68_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNX11933.1"
                     /translation="MENLFLEVQSAYNFDQTVQKLTETILSAGWKVTHVHDLQETMRK
                     NQFEVLPVKVMELCKPAYSVKLLQGDAERLYSSMMPCRISVYDKSDGNTYVSLMNSGL
                     VAASIGGVVEHVMTDAFNESQIFIQSIQ"
     gene            10559..11173
                     /locus_tag="PKH68_02990"
     CDS             10559..11173
                     /locus_tag="PKH68_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11934.1"
                     /translation="MKKYGIFVMAAMLTISLSITAQNAPENQNQNGRQAPQREQMTTQ
                     QRAENLAKQLNLTAEQTQKVQALYEKQDAKRAQQREEFRKNRQNMQQMRNAEREQMRA
                     QRDKEMKEEDAELESIIGKEKMDQVNAMRAQRRERAQQRMDNRGEGMRQGGNNRIGAG
                     PQGMNPGRENMRMGEGPKKEAVDTVKMNGKKAGKHLKKQQNKRK"
     gene            complement(11326..12576)
                     /locus_tag="PKH68_02995"
     CDS             complement(11326..12576)
                     /locus_tag="PKH68_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNX11935.1"
                     /translation="MRTEILAGITTFLTMAYILAVNPNILAATGMDKGALFTATAVAS
                     IVGTLAMSLLAKLPFAQAPGMGMNAFFAFTVCLGMGYSWQFALTAILVEGLIFLVLNF
                     FNLRAAIVDAIPATLKSAISAGIGLFIAFVGLQSAGIVVKNDATMVSLGHITSGTALL
                     ALIGLIITSVLLIKKVKGGLLLGIIATAIIGIPMGITKFTGFMDIPPSPAPLFFKFDF
                     AQIFTWDMLIVVFTFLFVDVFDTVGTLIGVCSKAHMLDEKGQIPNVKKAFYSDAIGTI
                     SGAVFGTSAVTTYVESASGVSEGGRTGMTSLITAGCFALALLFAPLFLSIPGAATAPI
                     LILVGLMMLDPIKEIDLTDFSEAIPAFMCIIAMPLTYSIAEGIALGLLAYVLLNVISG
                     KFKKVSVAMYILAVLFILNYIFIQ"
     gene            complement(12661..13362)
                     /locus_tag="PKH68_03000"
     CDS             complement(12661..13362)
                     /locus_tag="PKH68_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HNX11936.1"
                     /translation="MRKVLFALLFLSTVSVAQAQNLQVHYDFGETRKHVTTTFEMFKP
                     DKWGNTFSFIDFDFNYGDKNSPSGAYMEIARCLNFWGGPLSAQIEYNGGLGGYAIAPS
                     QYSGGSYAINNAWLAGVDYFIHSKDFSRTLNLKALAKNIVGKNYTPQFTGVWTVQMLS
                     KKLTFTGFADLWWEKDTYANAGTTANPVFISEPQLWYNFTPNISAGSEVEVSTNFAGN
                     EGFMVNPTLALKWNF"
     gene            complement(13823..14683)
                     /locus_tag="PKH68_03005"
     CDS             complement(13823..14683)
                     /locus_tag="PKH68_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNX11937.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIK
                     NGDRVALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQQNERKPSAEELAE
                     ELDIPVDKIADTMKVSGRHVSVDAPFVEGEDNSLLDVMINDDSPNADRVLINESLSKE
                     IERALSTLSEREHEIVKKFFGIGIPEMTLEEIGDEFGLTRERVRQIKEKAIRRLRQSS
                     KSKLLKTYLG"
     gene            complement(15041..15868)
                     /locus_tag="PKH68_03010"
     CDS             complement(15041..15868)
                     /locus_tag="PKH68_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNX11938.1"
                     /translation="MKSKLKIFILMLCLTAGNAFASKVDTVLTFSPSMKKNIKAVVIT
                     PDTYPKTDKMPVLYLLHGYSGKYDNWTKHMPNINYLADQYQFIIVCPDGNYGSWYFDS
                     PIDTTFKYETYVAGELIQWIDKNYKTISSPTGRAISGLSMGGHGALFLAFRHQDVFGA
                     CGSMSGGVDIRPFPNNWELAKRLGEQRTHPENWEKNTVINQLHLLTPGSLKIIFDCGT
                     EDFFYPVNVRLHEELTYRNIPHDFISRPGQHNREYWNNAVKYQAQYFSDFFKPGLNK"
     gene            complement(15969..17498)
                     /locus_tag="PKH68_03015"
     CDS             complement(15969..17498)
                     /locus_tag="PKH68_03015"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNX11939.1"
                     /translation="MNLSNKTKTIALFSAIVIVVSAATSYATTYISNKKRAVEFQPTT
                     EQTLSTPARFASATPALETDFTVAADLTVHAVVHVKTKGIISAGQQNFFDDPLFDFFF
                     GPQQQQQQRNNTPKEAVPLGAGSGVIISDDGYIVTNNHVIKDASQIDVTLNDKRSFTA
                     KLIGTDPSTDIALLKIDAKNLQPITFGNSDALKVGEWVLAVGNPFNLTSTVTAGIVSA
                     KARNIGIIGQNNFSQGQTNLSIESFIQTDAAINPGNSGGALVNTRGELVGINTAIASQ
                     TGAYNGYGFAIPSSIVSKVVTDLRKHGAVQRALLGVQIQDLTTEIAKEKDIKTLEGAL
                     VGDVSSDGAAEKAGVKKNDVINEINGVKVKSVAELQEQIGRYSPGDKVTVGIVRDNKQ
                     LKLNAQLMNADGNTDLVTASTTEADLGATLKPIDTKVKRALGIDYGLSVESITKGELA
                     AVGIRQGFIILKINNEPMETVKDFKNACNTALKKDKKLYIAGVYPTTGKVAYYEVKLE
                     K"
     gene            complement(17874..>18739)
                     /gene="eno"
                     /locus_tag="PKH68_03020"
     CDS             complement(17874..>18739)
                     /gene="eno"
                     /locus_tag="PKH68_03020"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNX11940.1"
                     /translation="FTLPVPMMNIINGGSHSDAPIAFQEFMIRPVGACCFREGLRMGA
                     EVFHSLKKVLHDKGLSTAVGDEGGFAPTLAGGTEEALESILLAIKNAGYEPGKDVMIG
                     LDCAASEFYKDGVYDYTKFEGANAKKRTSAEQVDYLAELAANYPIDSIEDGMAEGDWD
                     GWKLLTDKLGKKIQLVGDDLFVTNVEYLKKGIDMGCANSILIKVNQIGSLTETLDAIE
                     MAHRAGYTSVTSHRSGETEDSTIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEE
                     LGDLAVYGWKK"
BASE COUNT         5284 a         3865 c         3904 g         5586 t
ORIGIN      
        1 gtagcgtcag caggattgta actgcctacc ttgccatacg tcagacgcat ggtgaaattg
       61 gcattcggat atcttgccgt gttgttttgg gcagcaattt ctttcagccc ggcttcatag
      121 agtcgttctg cggtattgat tttctgtaag gcatcggcat atttttggtt gttgaacgtg
      181 ttcatcgttt tcaaaacgtc gttgtaaaaa acgatagccg gatctttttc aaaattatag
      241 cctttcttat tcagagcatt gcttagtcgg gccggtgaaa caaatgccga tttttcgaaa
      301 agataagcag cgtattttgc gtaattgcct ttgaattttt tatcaatggt gttgtaaatt
      361 tcgggcaacg catcggcagg aaccgatttg cggtacgcgt cgagcagtac gctgaatgta
      421 gcctgatcca cttcgggata ataatccttg taaagttctt cggctcgttg gagtatttta
      481 tccgaagttt cgttgttttt tagtccacgt tttaccatac tggcaatgcg tggcagttct
      541 acacccgaca acaacgattc gcgcagataa ttcagcgcat gactgtatgg gtaaatggcg
      601 ttgtagttat cttccagatc tttcagtaca ttgccgtatt tgctcacccg ttgggcatct
      661 gcttttaccc aggcagcaaa ttcagcttcc tgtttttgct tttgatccac cactttcagg
      721 tgttcgatgg catcgttcat cccgatgctg tttttccagt agtttgaact gcgggcatat
      781 ttgcctgcat acgctatgtt gattgcctcg ttggccagca tatatttttt ccaaacatcc
      841 tgttttgcac cccgaatttc gatgcgggct ttatttccgt tatttacccg ctcattgatg
      901 ccccacgagc tcaggtatcg ctcggtagag cccggatttc ccaaaatcat ggcgtaatcg
      961 ccatcctgat aaccctgatt ggaaatggtt acaaaccgtt tcgggctata gggtacgtta
     1021 tctttggagt attcagccgg tttaccatcg ggtgcgcaat aaacccgaaa taccgaaaaa
     1081 tcgcctgtat gacgtggata catccagtta tctgtttcgc cgccaaattt tccgatagac
     1141 gatggaggtg cataggcaaa ccgaacatca ttaaatcgtt cgtaaacgaa taaataaaat
     1201 tcgttgcctg aatagaacga tttcacctgt gcggtataat gggttccttt agcagcttca
     1261 tcgctgatag ctttgcaaac cgagtcctga acggcatcac gtttctttcc atccagattt
     1321 tcaatttgag gcaaaacccg gtcggttaca tcctgaattt taaccagaaa agtaacagca
     1381 agtcccggtg atggaatttc atccttcagt tcgttggctg taaatccgtc tttcaggtaa
     1441 ttatgttcta ccgaactatg tttctgaatg gcatcatatc cgcagtggtg gttggtgaat
     1501 acgagtcctt cgggcgaaac catcacaccg gtacatcctc ctccgaaaat gacaatggca
     1561 tctttcaggc taacattttt atcactgtaa atttcttcgg cagtcatggt gagacccatt
     1621 ccctgcattt tctgaatgtt gagtttttct atcaaaggca acatccacat gccttcgtcg
     1681 gctttcacac tgatagttag cagcaatgcg atcaaaaata aacttatttt tttcattgga
     1741 tttttttatc cacatccact aattgtggat ccggaaaatt ttattgttct tatttgattt
     1801 taataaattc tgaattataa aaaactactg acagatagct caaacactgt cagcagttaa
     1861 atttgttttc tggccggaaa tctgtttttt ttcgaaaaca tcctcattcc ttaccggaat
     1921 tcccttattc agggtttgta tcaacttaat ctacataagt aatccagccg tgtgtgtcgg
     1981 gctcgtcgcc gtactgaatg gcacgcagtt tatgatacag tttttcgctc catttgccgg
     2041 gttttccgtc tttggagatg atgtaggttt tgttttcctg caggtcgtcg atgcgttcta
     2101 tcggactgat aacggctgct gtaccgcaag ccccggcttc ttcgaaggtt gccagttctt
     2161 cttcgggaac cgcacgtcgt tccactttca tgcccagttc ttcggccagc accatcagac
     2221 ttttgttggt gatggagggc aggatagaag tagatttcgg tgtaatatac gtatcgtttt
     2281 ttattccgaa gaaattggca gcgccgcatt cgtcaatata tttcttttct ttcgcatcga
     2341 ggtagaatac ggccgaataa cccatgtcgt gagctttcat gccggctacc atactggctg
     2401 catagttgcc gcccacctta aatgtacctg tgcccagtgg tgctgcacgg tcatattcac
     2461 gcatgattac gtagggcgta gtggcaaatc cgcctttgaa ataaggtcct acgggagtta
     2521 cgaaaataag gaattcatat tcttttgcag gtttcacacc cacctgagcg cttgtaccga
     2581 taagtaacgg acgaatatag agcgatgcac ccgacccgta aggcggcacg aaccgttcgt
     2641 tcagtttcac cactttcagc acagcttctt cgaacatttc caccggcagt tttgccatca
     2701 taatgccttc gcatgaagat tgcaggcgtt tggcattttc atccaggcgg aaaatgcgta
     2761 ttctgccgtc ttttcccttg aatgctttca gcccttcgaa tgcttcctga ccataatgca
     2821 ggcaggtagc tgccatgtgc atcgtgatat tctcgtcttc acatgttctg atttcgcccc
     2881 acttaccgtc tttgtaagta cagcgaacgt tgtaatcggt tttcatatag ccgaaaccca
     2941 aattgctcca atcaatgttt tccataattt aataaataag ttgcccccta accccagcct
     3001 ctccctattc cgaagcttcg gaaccctccc caaaagggag ggacttttaa agaggggaat
     3061 ttaagaacgt ttataaattt tgttatttta tttttactag tttaattctt atgtcaattc
     3121 attagaaatt aatgattttt cagcatttat tcccctttta gagcctgtcc cnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattcattag aaattaatga tttttcagca
     3301 tttattcccc ttttagagcc tgtcccgaac ttgattcggg agggttaggg ggcaggtctt
     3361 ttatagtatc atcactgcct cattccccat attaaacaac agatcgatga tgcttaaatc
     3421 gggcaaaaat ccatacttgg tttcgaaaac ctgataatag ggcttagtga ccaaaaccgg
     3481 ctccttttta ggatgaatta tctcgcgtaa atcgttgaaa tcgaacaaat tattctgata
     3541 tgtttcagtc ggtataattt ttggattttc attcagtaat ttcagtatca aagttagcaa
     3601 ttcaagattg taatcccaaa gaaaagtcca tttattttca tagaaaggtc ggaaatcatc
     3661 ctgataatac tcaaagaacg ggctgttcat gtaagccgat gtaatcgccc gccagtgagt
     3721 ttgctgccag ttgccgtgag atgaaattct gacgtcgcga ataggatttt tctgtccgct
     3781 gttactctcc accggaatgc tcagtgtctc cacaccattt gctgtagcta tccggcagcg
     3841 gtttcggtac gattgtttta cgtaattctc acaagcctcc agccatatct cgtttgcatg
     3901 caacatacac atgaagtaag aaacgggtgg gagataggca gtcgagagca tggccctgct
     3961 tcctaactct cccgaatcaa gtttcccgat agatcgggac atgcagggac tagctctgaa
     4021 tgtggaactt aaaagtgctg atttgaagct ttgtactgca tttgatggta tgctgtcaac
     4081 acccccttca gggggttggg gggcagcctg tgtcttatcg ttctgcattc ttaaaaaatc
     4141 tgtcaaaccg gattttgccg ttgaagagtg atttgtcttt gttcagtgaa agccagacaa
     4201 acatggggcg tcccactatg tgatcctctg gtacaaagcc ccagtaacgt gaatcggcag
     4261 atttatgacg gttgtcaccc atcatccagt aatagttcat tttaaaccgg tagcttgtgg
     4321 tcggttggtc gttgataaat atctgaccgt cgcgcacttc cagtttgttt ttttcgtaca
     4381 cgcggatgat gcgttcgtat ataggcagat tggcaagcgt gagtttcaga acagcaccgg
     4441 ctttaggaat gtaaataggg ccatagttgt cacgcgtcca gttataagta tttccgagcg
     4501 ggaaagtatg atcttccatt aaaattccgg acggctccac caccagctta accacaccgc
     4561 gggtattttg catctttgaa tacgattcgg cggtcaacgg aaaattataa accggcaaat
     4621 ttggattaag ccctaacgaa gtaatcaacc ccgcagcctg tgaattgtcc agcagcacaa
     4681 tgtcttcatt actgatattc atatcttcca atacttcatt ggtcagtcgt gttccatctg
     4741 tttgtacaaa atagttgtac tgtatccccg ggaaatgctc ctgtggttta ccatttacaa
     4801 ttacctgatt gtcttttact tccagccagt cgccggcaac tcccacacat cgttttacgt
     4861 agttttcacg ccggtcaacg gggcggtaaa caatttcacc gaaaatatca gtgcgcgaac
     4921 gaacaacatc ccgtccgtac agaaagcaca aggtgtaata atccggattt tgctgcttta
     4981 cacacacggt atcaccggtg ggaaaattaa atacaacaat atcgttacgt tccagtttat
     5041 cggttccttt caggcgtttg tattcccact gcggtttttc gagaaacgat tttgagttga
     5101 gaatggggaa tgtgtgctga accagcggga atgaaagtgg cgtcatcggt acacgcggac
     5161 cgtaactagc tttgcttaca gccagaaaat cacccacaag cagcgttttt tcgagcgagg
     5221 aggtgggtat ctgatagttc tgaaacaaat aagtgtgaat aaagtacaca gcaaccaatg
     5281 caaaaacaat ggcatcgacc caactcatga tggcgagtac cgttttgttt tttgattttt
     5341 tccaccaggt ccagggtatt attttggtga tataaacatc gaaaatgaat ggcagtaaca
     5401 gtagccacca gaaattacca acccatgaaa cgaatagaac aaatataagg ctccagaaag
     5461 cgcatttaat ccactgtttt gccggctttt taaatcttga agctgttatt gcggtgttca
     5521 tataaatgga atttaagttt tagtattaat attttaattg agaaataatt cagaaattca
     5581 gcatatcatt catgcctaga aaaccttttt taccgcacgt aaattcggct gccagtacgg
     5641 caccgagcgc aaaaccttcc cgactttttg catcgtgtgt tatttctatg atgtcggcaa
     5701 atgattcata ccggatagtg tgtatgcccg gaacttgtcc ttcgcgaatg gatttaatgg
     5761 caagttcatt gtcattttgt tgtatctctt tcacccagcc tgttttccgg tcaatattgt
     5821 caagaattcc ttcagcgagc gttatcgccg tacccgatgg tgcatccagt ttctgtatgt
     5881 gatgcacttc ggtcatttct acgttatatt caggaaaccg attcatgatt ttagccaggt
     5941 atttgttaac tgccatgaaa atatttactc cgaggctaaa attggaggac caaaagagtg
     6001 tataaccgtt tttcagcacg tcggctttca ctttttcctg ttgatcagcc caacctgttg
     6061 tacccgagac tacagctaca ccggcttcaa atgcctgttt ataattgtca agggcaacct
     6121 gcgggacagt aaattcgata gctacatcag cgcttttgaa agcatccgat ttgaaatcat
     6181 cccggttgtc aatgtcaatg attgaaacta tttcatgtcc gcgttcgagg gcaatccgtt
     6241 ctatgatttt gcccatttta ccatatccta ttaatgcgag tttcataaat atgtctgccc
     6301 cctaaccccc taaaggggga attgtagagc ttttattttg taagttaatt ctatatgaaa
     6361 aaatttaaat gctaatattc aagaatagat tttatttaac aaaacaatgc catcccccct
     6421 ttagggggtt agggggcagg cctgtctgct caatattgcc ccgttcccaa taacacgagt
     6481 gtacaagcca cttctgcttc gatgttgttg tctttcagga ttttataaaa ttcctcgttt
     6541 tccgttccgg gattaaatat caccctgcgt ggtttcagcg aaaggatata atcgtaatac
     6601 tcaggctgcc gttgaggtcc aacgtaaagt gtcacggtat caatcgattc gtagtttttc
     6661 ttttcggtgt caatttcgcg gtcaaatatc acgtccggtc gaagtccgag taactctatt
     6721 tcgtggttgt gatgcagcag acgctccgct gctttgtacg aataccgttc gggattttta
     6781 ctggcaccta ttattaatgt tttcataatt aagttattta gcccccctgc cccctcccgc
     6841 cttgcgggat aaactgcagg gggttcaagg aaaaacaatt attggttatt gagttataag
     6901 ttcagaaaca tcaggattta gttttggaat ttctaatttc taatttcatc attctttaaa
     6961 aaagtatgtc caggcattcg tatatttctt cttttgtggc tgtctggtta atcctgattt
     7021 cgcccacatc cgacaataag gtgaaattta tctcctgatt gtcattcttt ttatcgtgca
     7081 tcatcagttc aaggattgtg tcgtaattgc tgcaatcgca attgaatctt ccgtaatttt
     7141 cctgaacaaa atgcctgtat ttctgcaaat cttctttcgg aaagccgagc ttgatatgcg
     7201 aaagatacaa ttcgcaaatc attccgtaag ccacagcata gccgtgaagc agaggttggg
     7261 cagttttata cgattgagtc tcaaatgcat gaccgaaggt gtgacccaga tttaaagctt
     7321 tgcgtattcc ccgttcggtc gggtcttcag ccactattcg ttgctttatt ccaatattat
     7381 aggctaaaag ttcttttaaa ttttcaaaat cgatattgtc gaaatcaaat gcgaggacct
     7441 cattccaggc acgacgtgag tctatcagtg catgcttcac catttcagca aatccggaga
     7501 gaaaattccg ggcatcgagc gtacgaaaaa aatcaacatt gaccaaaacg gtgcttgcag
     7561 gcgaaaaaac gccgatttca ttcttcagac cccgagagtt aattccggtt ttacctcccg
     7621 tggcagcatc tacggcacca agcagggtag tggggatgtt gatgtaactc agtccccgct
     7681 taaaggtgga cgcggtaaac ccaccgatgt cggtaatcat tccacctccc agattaatca
     7741 tcagcgattt gcgggtggcg ccattttcgg tcagcaatgt ccagattttc acagccgaat
     7801 caatgttctt atgattgtcg ccgtgttcga tggtgattat atgacttcct tcagccatgc
     7861 tgcatttcat cacttcgggc aggcagaact tttcggtatg ttcatctgtt agtatgaaaa
     7921 ttttgttccc ctgccattca ttcaggagtt gattgagttc agcagtaaag tcggaagtaa
     7981 taatggtctt tgattccgtc atcgcatttt tttttagccc acaaagatac attatttctt
     8041 aaaattatct ttttatttct gctgaaaatc agccccgttt taagcttata tagctaatac
     8101 ggaaaataat atattaaaaa atatgaaatt attttgaatg tatgaagata tgttcataca
     8161 tttgcagaaa attattcgaa ccatgttacc agataatacc gaaaaaatgg aagaagcagc
     8221 ttatatgtta agagcaattt caaacggaac ccgtttgcag gtgattgcca ccttgtctga
     8281 aaaggaggag ttgagcgtat cccagcttca ggagcaactg aactgcgaac aatcacttct
     8341 ctcgcatcac ctcaccgata tgcgtgccaa gggtgtactc aattgcagac gtgaaggcaa
     8401 aaactgttat tattcgctca aaaacaaaca aataattcag atattgaatt gtatcaattc
     8461 atgcaactgc aattaaatat ttattaataa caaaatgaaa aactgaagta tgatgatcgc
     8521 ctttttgaaa aaaaacatac tgcctataat cggactactg gtgggtgctg ccggtggata
     8581 tctatactat tactatgtag gatgtgcttc cggaacctgc cccttaacct ccaatcccta
     8641 catcagcgtg ttgtatggag cattgatggg ttttctgctg ttcgatatat ttaagaaaaa
     8701 agaaaaataa aataaatatg tccaagtaaa tatttttgac aaacacgggc atgataaaaa
     8761 aactcacaac aaatgataga cgctccatat tcctccgtca gctgacggat ttgggggccg
     8821 ataaatagat tataaacaaa tgaaacaatt gaaagactat tttagaatgt gggattttgc
     8881 ccgctggttc aaactgacat tagcagtagc attgggactg gcttatgtgt ttggaagtgg
     8941 cagcatttat ctgttcggat ctttgttttt tggcgttcag gcaatactca acatgggttg
     9001 cggatgtgcc accggcaatt gcactacaac cgtgaaaaag gatgagaagc agataagtta
     9061 cgatattgaa gaatataaac caaccaaata atatgtacag tacatttctg gtgaatggtg
     9121 aggcatgtct cacctgcaac aatcaggtgt tgaagaaaat cggaactttg cagggcgttt
     9181 tcggagctga aatagatcga attcgcggtg aagtggtcgt gtcgcatacc gacgaggtaa
     9241 gccgtgaaat gatagagaaa acactcaacg aacttggatt ttttgaagaa aataacagtg
     9301 ataataattg cgaaataaat acttaaaaaa aatattgtaa tgaaaaaatt agctttagca
     9361 accactttac tcatctcatt agcgatagtg tcctgtgccg ggaccagtaa agacaaggaa
     9421 aaaaaatcag gcacaacagt acaaactgaa acaaaaaaag tagaaaataa taaaactgaa
     9481 acaaaaaaaa tgggaacaat tcatttaaca aaagaagaat ttcttaaaaa agtagtaaac
     9541 tacgaaacca attcaaaaga atggaaatac ctgggcgata aaccctgttt gattgacttt
     9601 tatgccgact ggtgtggacc atgcaaagtg gtggctccaa tcctggaaga ccttgccaca
     9661 gaatatgccg gacaaattta catttacaag attaataccg acgaagaaca ggaactggct
     9721 gctgctttcg gtattcgcag catcccttca ctgctttttg tacctatggg cgaaacaccg
     9781 caaatgacac agggtgcaat gccaaaagct aacctgaagg aagcaatcga caatgtattg
     9841 ttgaagaaaa aataaatttc aaggtgtagt aagcagaaaa tccggttttc gatgattata
     9901 tccgtataat gattgatcaa ccggattttt tttcataaaa gagcaaagag caaagagcaa
     9961 agagcaaaga ataaagacaa aagacctgtc atcgttttgc aggtatcaat aactcaataa
    10021 ctcaataact caataactta ataacttaat aaccaaccaa atggaaaatc tatttttaga
    10081 ggttcagagt gcatacaact ttgaccaaac agtgcaaaaa ctaaccgaaa caatactctc
    10141 ggccggctgg aaagtaactc atgtgcatga tctgcaggaa actatgcgta aaaatcaatt
    10201 cgaggtacta cccgtgaaag tgatggaact gtgcaagcct gcctattcgg taaaattgct
    10261 tcagggtgat gccgaacggc tttattcttc catgatgccc tgccggattt cggtgtacga
    10321 taaatcggac ggcaatacct acgtgtcact gatgaattca ggtctggtgg ctgcctcaat
    10381 tggtggagtg gtagagcacg ttatgaccga tgcttttaat gaatcgcaaa tatttattca
    10441 atccattcag tagtactaag tgttaaattt gtaaaaattc taaggcataa ttaaacgttt
    10501 tgatttacca ttcgtcaaag caataaacga tttaattatc aactttaaca aaatgaatat
    10561 gaaaaagtat ggaatttttg taatggcagc aatgttgaca attagtttgt cgatcacagc
    10621 ccagaatgct cctgaaaacc aaaatcaaaa cggcagacag gctccgcaac gtgaacaaat
    10681 gaccacacag caacgtgctg aaaatctggc caaacaactt aatcttacag ctgaacaaac
    10741 tcagaaagtg caggctctct acgaaaaaca ggatgccaaa agagcacaac aacgcgaaga
    10801 attcagaaaa aaccgtcaga atatgcagca aatgcgaaat gctgaacgtg aacaaatgag
    10861 agctcaacgc gataaagaaa tgaaagaaga agatgccgaa ctggaaagca ttatcggtaa
    10921 agaaaagatg gaccaggtga atgcaatgcg tgcacaacga agagaaagag cacaacaaag
    10981 aatggataac cgtggagaag gaatgcgtca gggtggcaac aatcgaatag gagccggacc
    11041 acagggaatg aaccccggca gagaaaacat gcgaatgggc gaaggaccta aaaaagaagc
    11101 ggttgataca gtaaagatga acggtaaaaa agccggtaaa catttgaaaa agcaacagaa
    11161 taaaagaaaa taagtttgtt tcttgttttg atttgatttg atttgatttg attgaatgac
    11221 taattgatta atgtaaaaac ggctccaatg ttcatcgggg ccgttttttt atgttggctt
    11281 ttccaaaaat actttttgaa agaaatgaat ttgaaaaaaa agtttttact gaataaaaat
    11341 ataattcaga ataaacaata cagccagaat atacatggca actgatacct ttttgaattt
    11401 tccactgatt acattcagca gcacgtatgc caaaagtcca agtgcaattc cttcggcaat
    11461 gctataggtc aagggcattg ctataataca cataaatgca ggtatagctt ccgagaaatc
    11521 ggtcaggtca atttctttga tcggatcaag catcataaga cctacaagta taagtattgg
    11581 tgcggttgca gcacccggta tcgaaagaaa caggggcgca aataacaaag caagtgcaaa
    11641 acagcccgcc gtaatcaacg aagtcatacc cgtacgacca ccttcagata ctccggaggc
    11701 actttcaaca taggtagtaa ctgcacttgt tccaaatact gctccggaaa tcgttccgat
    11761 agcatcggaa taaaaagctt tcttgacatt tggtatctga cctttttcat ccagcatgtg
    11821 ggctttggaa catacgccaa tgagcgtacc tactgtgtcg aacacatcga caaagagaaa
    11881 ggtaaacact acaatcaaca tatcccaggt gaaaatttgt gcgaaatcga atttaaaaaa
    11941 caggggagca ggacttggcg gtatgtccat aaaaccggta aatttggtga tgcccatcgg
    12001 aatacctatt attgctgtcg caataatacc gagtaataaa ccacctttca ctttcttaat
    12061 caaaagaacg gatgtgataa ttaatccaat aagcgctaat agtgcagtac ctgaagttat
    12121 atgaccaagt gaaaccattg tggcgtcatt tttgacaacg atacctgcac tttgcaaacc
    12181 aacgaatgca ataaataaac caatacccgc actgattgcg ctctttagag tggcaggaat
    12241 tgcatcgaca atagctgcac gcaaattgaa gaagttcaac accagaaaaa tcaaaccttc
    12301 aaccaggatt gccgtaagtg caaactgcca ggaataaccc atgcccagac aaaccgtgaa
    12361 agcaaagaaa gcattcatgc ccataccggg agcctgagca aatggtaact tagccaacaa
    12421 tgacatagcc agagttccta caatggaggc aacagccgta gccgtaaaca gagctccttt
    12481 atccatccct gtcgcagcca ggatgttggg gttcacagcc agaatatagg ccattgtcaa
    12541 aaaagtggta ataccagcaa gaatttcggt tctcatggtt gtaccgtttg cctttaaatg
    12601 gaaatatttt tcaaacatat atctgatttt ttaattgtaa tagattttgt tatcccgatt
    12661 ttagaaattc cattttaagg ccagggtcgg attgaccata aatccttcat ttccggcaaa
    12721 attggtagaa acttcaactt cactgcctgc cgagatattg ggcgtgaaat tataccaaag
    12781 ctgaggctcg gagataaaaa ccggatttgc tgtcgtacct gcattggcgt aggtgtcttt
    12841 ttcccaccaa agatcagcaa aaccggtgaa agttaatttt ttactgagca tctgaaccgt
    12901 ccaaacaccg gtaaattgcg gggtgtagtt tttcccaacg atgtttttgg ctaatgcttt
    12961 cagattgagt gttctcgaga aatctttact gtgtataaaa taatcgacac cggcaagcca
    13021 tgcattatta atggcataag agccgcccga atactgtgat ggagcaatgg cgtatccgcc
    13081 aagtccgccg ttgtattcta tttgtgcgct cagtggaccg ccccaaaagt tcagacaacg
    13141 ggcaatttcc atataagcac ccgaaggtga atttttgtcg ccgtagttaa agtcaaaatc
    13201 gataaaggaa aaggtatttc cccatttgtc tggtttaaac atttcaaatg ttgttgttac
    13261 atgctttctg gtttctccaa aatcataatg aacctgaaga ttctgtgcct gagcaactga
    13321 tacagtactc aaaaataaca gtgcgaaaag tacttttctc ataaactgat gtttttttta
    13381 atttaattat tattgaatga ataaagatga aattctcatt ttattgattg accgaaaaaa
    13441 aggagatatc tgttcggtat tacatttttc aaaaattctt tttaacaatt cggaagataa
    13501 gattctgtaa aaatatcagg tataagataa atcacaaagt ctataaaaca gataaacagg
    13561 aaggcactaa gacaaagaga taggttcagg catagttgta tgccggttat atgtggcaac
    13621 ttacccggct gcaaaagtag gaaaattaat ttaaattttg attaagccga agagttaata
    13681 ggataatata actcatctga ttaattatca gcattatgac atgagatggg atgaaaaatt
    13741 gtcgctaacc ggttaaaaat aaaaaagcaa ctccggttaa ggtgttgctt agtaatttgt
    13801 atgttcccaa catgtaattt ctctaaccca ggtaggtttt cagaagtttg ctttttgagc
    13861 tttgtctcaa tcgtctgatt gctttttcct tgatctgacg tacacgttca cgtgtaagtc
    13921 cgaattcgtc tccgatttcc tcgagtgtca tttcaggaat accgatacca aagaattttt
    13981 ttacaatttc gtgttcccgc tcgctgagcg tagataatgc acgttcaatt tctttggaca
    14041 gagattcgtt tatcaacaca cggtctgcgt tgggagaatc gtcattaatc atcacatcga
    14101 gcaagctgtt gtcttcacct tctacaaagg gagcatctac ggagacgtgg cggccggaaa
    14161 ccttcatggt atcggcaatt ttgtcaaccg gaatgtccag ctcttcagcg agttcttcgg
    14221 ccgaaggttt acgctcgttt tgctgctcaa atttggaaaa tgctttgttg attttgttca
    14281 atgagcctac ctgattcaac ggcaaacgta cgatacgcga ttgctcggcc agggcctgaa
    14341 gaatggactg acgaatccac catacagcat aggagataaa cttgaaccca cgtgtttcgt
    14401 cgaatttttc agcagctttt atcagaccca ggttaccttc attgatgagg tcggggagac
    14461 taagtccctg attttggtac tgtttggcta ccgaaactac gaaacgcaaa tttgcacggg
    14521 taagtttttc caatgccaca cgatcgccgt tcttaatacg ttgagcaagt tctacttctt
    14581 cttcaacagt aataagatct tcgcgtccta tctcttgcag gtacttgtca agtgatgcac
    14641 tctcgcggtt tgtaattgat tttgtaattt ttagttgtct catataggga ggaaaaattt
    14701 agcccgcaaa ggtaatgaat tttttaatca tttgaaaatt aataacgtga ataatttttc
    14761 aggtgtttct ttattttttt tgtgatttta agggttaata actttcattt ttaaaaggtc
    14821 aaacagtttg tcagccaacc gtgaaagagt ttgaaagacc aatttttttg atatttttgg
    14881 tgtttgcaat aatctcactc atttataagc agaacgtatc taagcgttct ttgttgcgac
    14941 aaaccatttt aatttgtcaa taagctttgc agtttattga tacaaattta taacaaaaat
    15001 agtaagtttt tgtttttgaa atgcagcaat tgtgtaacaa ttatttattc agccccggtt
    15061 tgaagaaatc ggagaaatac tgagcctgat atttcacggc attattccag tactccctgt
    15121 tgtgttgtcc cggccgtgaa ataaagtcgt gtggaatgtt gcggtaggtt agttcttcgt
    15181 gcagacgaac atttacgggg taaaagaagt cttccgttcc acagtcgaaa attattttca
    15241 gactgccggg tgtgagcagg tgtaattgat tgattactgt atttttttcc cagttttcag
    15301 gatgggttcg ctgttcgcca agccgtttgg ccagttccca gttgttgggg aaagggcgaa
    15361 tatccacgcc gccgctcatg ctgccacaag ctccgaatac atcctgatgg cggaaagcca
    15421 gaaacaaagc tccgtgtccg cccatactga ggccggaaat ggcacgtccg gtggggctgc
    15481 taatggtttt gtaattttta tctatccatt gaataagttc accggcaacg taagtttcgt
    15541 atttgaaggt tgtgtctatc ggactatcga aataccagct gccatagttt ccatcggggc
    15601 aaacaatgat aaactggtac tggtcggcca ggtaattgat gttgggcatg tgtttggtcc
    15661 agttatcata tttgccgcta tacccgtgaa gcagatacaa taccggcatt ttatcggttt
    15721 tcgggtaggt atcgggggtg ataactaccg ctttaatgtt tttcttcatc gaaggactga
    15781 aggtgagcac tgtgtccact ttcgaagcaa atgcattgcc ggctgtaaga caaagcatta
    15841 gaataaaaat ttttagtttt gatttcatgg gattgtgttt aattttttca aaggcagtaa
    15901 aaataagaaa aaagactgac atttcatttg aaagccagtc ttttttttaa catggaaatt
    15961 tcaactgttt atttttccag tttcacttcg tagtaagcca ctttgccggt ggtgggatat
    16021 actccggcga tatagagttt tttatctttt ttcagtgctg tgttgcaggc atttttaaag
    16081 tctttcaccg tttccatggg ttcattgttg attttgagaa tgataaaacc ctggcgtatg
    16141 cctactgcag caagttcacc tttggtgatc gactctactg ataatccata atcgatgccc
    16201 aatgcgcgtt ttactttggt atcgatgggt tttagggtag ctcccagatc tgcttctgtg
    16261 gtagaagccg ttactaaatc tgtattacca tcggcattca tcagctgggc attcagtttc
    16321 agttgcttat tgtctctcac aattcctacc gtaactttgt caccgggact gtatcgtccg
    16381 atttgttcct gtagctcagc caccgatttt accttcactc cgttgatttc gttgatcacg
    16441 tcgttctttt tcactccggc tttttcggct gcgccatcgg atgaaacatc acccaccagc
    16501 gcaccttcaa gggttttgat gtctttttcc ttggcaattt ctgtggttaa atcctgtatc
    16561 tgtactccca ataatgcgcg ctgtactgct ccgtgcttgc gtaaatcggt tacaaccttg
    16621 ctcacaatgc tcgaagggat ggcaaatcca tagccgttgt atgcgcctgt ttgtgaggca
    16681 attgctgtgt taatacccac cagctcacca cgggtattga cgagtgcacc gccgctgttg
    16741 cccgggttga tagctgcatc ggtttggata aaggattcga tcgacaaatt ggtttgtccc
    16801 tggctgaagt tattttgtcc aataattccg atatttcggg ctttggcgct tacaataccg
    16861 gctgtcacgg tcgaagtcag gttaaacgga ttgcccactg ccagcaccca ttcacctact
    16921 ttaagcgcat cggaattgcc gaatgtgatt ggctgcagat tttttgcatc aatcttgagc
    16981 agggcaatat cggtcgaagg gtctgtaccg atcagtttgg cggtaaaact ccttttgtca
    17041 ttcagtgtga catctatctg gcttgcatct ttgattacat gattgttggt cacgatatat
    17101 ccatcatcgc tgatgatgac gcctgaaccc gctccgagag gaacagcttc tttgggtgta
    17161 ttatttcgtt gttgctgttg ttgttgcggt ccgaagaaaa aatcaaataa aggatcgtca
    17221 aagaaattct gctgtccggc cgaaataatt cctttggttt tgacatgaac cacagcatgc
    17281 acagtgaggt cggccgctac cgtaaagtcg gtttccagtg ctggtgttgc gctggcaaat
    17341 cgtgccggtg tactcagcgt ttgttcggtg gttggctgaa attcaacagc ccgtttcttg
    17401 ttcgaaatgt aagttgttgc ataacttgtt gcggcactta ccactattac aatggcagaa
    17461 aaaagtgcaa tcgtctttgt tttgttactt aagttcatac tcttttttat gtttagttgg
    17521 tttgtaatca tttacttctg aaaacacccg cgtgaaggta ttttcgatac gaaaatatac
    17581 aaactatatg cccgatgtgt cagttgaccg gtatatttct tcaaatttaa caatgttaaa
    17641 atgccgttta acgggcttta acgcagaaag atagcattat ttaaaaagta taaatgaaaa
    17701 catgacaaaa ttgcagtctt atgtttttcg ctttgaaatc tgaggctgaa attatgtcaa
    17761 tggctgacag aattgcagga tttaaaaaca aaaacagctg ccaatcctca gatcaacagc
    17821 tgttttctag ctttctttct taaaagtccc ccttggggga tttagggggc tgattacttt
    17881 ttccagccat aaacagccaa atcgcccagt tcttcttcta tacggagaag ctggttgtat
    17941 ttagccatac ggtctgagcg gctcagtgaa ccggttttga tttgtccgga gttggtagct
    18001 acagcgatat cggcgatggt tgaatcttcg gtctctcctg agcggtgcga tgttacagag
    18061 gtgtaacctg cacggtgagc catttcgata gcatccagtg tttcagtcaa cgaaccgatt
    18121 tggtttactt tgataaggat agagttagcg caacccatat caataccttt tttgaggtat
    18181 tctacgttgg ttacaaacaa gtcatcgcca acgagctgga tttttttacc aagtttgtca
    18241 gtcagcaatt tccatccgtc ccagtcgcct tcagccattc cgtcttcgat agagtcgatt
    18301 ggataattag cagcaagttc ggccagatag tctacctgtt cggctgaagt acgtttcttg
    18361 gcatttgcac cttcaaattt agtgtaatca taaacaccgt ctttgtagaa ttctgatgca
    18421 gcacagtcaa gaccaatcat tacatcttta cctggttcat aaccggcgtt tttgatagcc
    18481 agcaggattg attccagtgc ttcttcggtg ccgccggcca gggtaggagc gaatccacct
    18541 tcgtcaccaa cggcagtgct aagacctttg tcatgtagta cttttttcag tgaatggaat
    18601 acctcagcac ccatacgcag cccttcgcgg aagcagcatg cacctacggg acgaatcatg
    18661 aattcctgga aggcaattgg agcatcggag tgagaaccac cgttaatgat gttcatcatt
    18721 ggaacaggca gagtgaaag
//
LOCUS       DAOPZK010000028        18380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3177_length_18380_cov_18.386467, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000028 DAOPZK010000000
VERSION     DAOPZK010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18380
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3177_length_18380_cov_18.386467"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1592)
                     /locus_tag="PKH68_03025"
     CDS             complement(9..1592)
                     /locus_tag="PKH68_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HNX11941.1"
                     /translation="MVLNIGFNAEAKNHYQYLYKDLPFSMPLVKVPVFKKNMVSVEEF
                     GGVGDGITLNTNAFAKSMDALAAKGGGTLIVPKGIWFTGPIVFRSNINMHLEKGAVIL
                     FSPDFNLYPIVETIFEGLDTRRCQSPISGRNLVNVAITGQGSINGYGEAWRPLKKNKV
                     TASQWKKMIGAGGVVKNNDTWYPSESSFKGSQISDMNVPRQNLTEQEWLEIKDFLRPV
                     MVSFIECKNVYLQGVLFENSPSWNLHPLMCENVILDGLFVRNPGYSQNGDGLDLESCR
                     NSIIVNCTFDVGDDGICIKSGKDEPGRRRARPTENVIVDNCKVFQGHGGFVVGSEMSG
                     GVRNISVKNCQFLGTDVGLRFKSTRGRGGVVENIYISDIYMFNIETESFLFDLYYGGK
                     SAVETLEDGDVTPTEEKLLPVTEETPVFRNIFVKNLVSRNARRAMLFNGLPEMKINNI
                     QIEDINITAQYGAELSNSKDIVFKNVHIVPQNGSALILKDVENFISENFTYPSGLEKP
                     IDLKQNGNSNISISGKKYK"
     gene            complement(1752..3407)
                     /locus_tag="PKH68_03030"
     CDS             complement(1752..3407)
                     /locus_tag="PKH68_03030"
                     /inference="COORDINATES: protein motif:HMM:NF027642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5123 domain-containing protein"
                     /protein_id="HNX11942.1"
                     /translation="MKKYKNRTYGTILILLMIVGVFSACKEEEPELQLPYMFRPINFN
                     VELNKTVATFSWAKVDSAVSYTLQLSNDSLDYSKPLLDVTTTGLSLVKELAGDTKFYA
                     RIRANAADAAKSSKFNTALSFKTPKENLFQGYGTSINTGVVYSAYMTDFNALTVKWTP
                     AANVTHLILTNENATAKDSVLISAAEALAGSKVVSSLSNSVWKVRIFNNSILRGTTYG
                     TVEGDILVRSGEDLQAALNSAADGQVIVLEGGAVYNVGSGTYRFNKSVKLRGTLTSTL
                     PVVCMTVGTGTPPSATSSMLGFVDGSSMQSVKFENIDFTGYVDNNTAATKIGYLFNNN
                     TMTTVSKLSFANCNMHNFGNTPMRVQGAKNQVIDSLSINGCKINDIGFSSTYAIVNSN
                     SADLINAIEIANSTIYNFKGSLVLRTGQTLKSVKVINCNIDHGMQDAGSARYLMDFNN
                     ASFTGGGVTISNCVFGQTGAAMGANGIRYVAGTSVSIKGSYFTTDYVDDPIPAGATST
                     SIKPNMTSYKSASTALWTDPVNGNFKLLDTSFGGKGVAGDLRW"
     gene            complement(3448..5298)
                     /locus_tag="PKH68_03035"
     CDS             complement(3448..5298)
                     /locus_tag="PKH68_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX11943.1"
                     /translation="MKRLSKINILAGIAALMLSFSSCESFLDVPSNSLISDDVIFSQP
                     DSAYKAISAIYSIIGENNSYRNRLWLQMGVNTDIEFRSGWSSGAVLSSIKSDDLFALY
                     NPNSSIGDGYNNTDAANPWSRIYQGIERANLAIEGIRKFGNPAEGNDMGHLLGSALTM
                     RAYFFYDLIKWWGDVPPRFEPVSEATIYLAKTDRDTIYNQIIKDLQEASTLLYAPGTK
                     YTNTVKMISRDAARGLLARICLSAAGYSQRPSGTDAVIKITASDSRRRELYTIARDAC
                     RAIIADGHYKLDPDFKNIFYEQCQDVETHGREAIWELPYNLGIRGRMVYNLGLSREAD
                     GKNNKVALSPQFRIMPNFYYDYNVNDKRRDITVVPFKVVKNATTGVMEQSLSSGVTGF
                     NLGKWRAEWVKIPISGTDDGVSPIVLRYADVLLMFAEADLFLGGSEGAGYFNQVRRRA
                     FGTTAATYDLPLTLANIKMERAFEFCGENIRKYDLERWGELKASIDKAKASLIALRDG
                     TGDYATVPSVIYYKYVVDNSITTGERVLIVYGLQRGETDDKTVTDPTGGWTKKTWTNA
                     TTSTGELYLSDGFINNMYLGNPDKRQLLPIMHQIILASNGSLVNDYGYDN"
     gene            complement(5311..>6366)
                     /locus_tag="PKH68_03040"
     CDS             complement(5311..>6366)
                     /locus_tag="PKH68_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11944.1"
                     /translation="GVGYTYQWQNAASTSNRGAELSLNIGLVESKNFNLNFMFNISAN
                     QNKVDDLGGLDSYSFNEAWTSMTEASNSYVVKPGEPVGQIYGYVNEGWYSAKDFMWNG
                     TKWVMNSGKYNKYDAATKIYSDQYGNKFVDNSSIDGLSWGPGAMKLKDQNDDGKISDL
                     DRVVIGNTNPAHFGSFAFSGDFRGVDFALNFNWVYGNDIYNANKIEMSSFYYKYRNML
                     SSNLNSYTTMNWETGKRITDVNELDLMNANSHASIWAPPTGRYAITSWAIEDGSFLRF
                     NNMTVGYTLPTRLLKGLYIQKLRLYATAYNIYTFTNYSGYDPEVDTRRATPCTPGVDY
                     SAYPRSRSYNFGINLTF"
     assembly_gap    6367..6466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6467..8337)
                     /locus_tag="PKH68_03045"
     CDS             complement(<6467..8337)
                     /locus_tag="PKH68_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNX11945.1"
                     /translation="MQKHKIRVRHIALMLGMLLLSTNIVAQQRTISGTVTDQNGDPVI
                     GATVIVKEGTQGTVTNVDGKYTLGVPATARTLIFRSVGKINKEVDIAGSVVNVVLEDL
                     LKTLDEVVVIGYGTQRKRDLTGSISSVSEKALKDIPVATVAEALTGKLPGVQVTTTEG
                     SPDAEIKIRVRGGGSITQSNSPLYIVDGFLKDDIKDVAPSEIQSIDVLKDASSTAIYG
                     SRGANGVIIITTKSAKQGKISLSYDGYVGFKNITKKLEVLSPYQFVQKQYERATWNNA
                     VASEYEKYFGSYEDINLYNYKNGTNWQDETFGRTGFQQNHSVALSGGTKQLKYNFSYT
                     NLSDKAIMAMSDYNRNNFSGKLNYQPLKWLKLDVAARYAATVINGSGANDVTGSEKST
                     SDSRVKNAVIYTPIELKNMVAQDDDMDAVASLYSPLVTTADNNRYQKKRDYNINGGFT
                     VNFTKNLSLRSTYGLTKSNKDDKRFYGVTSYFAREGGALKRDNAVAPSILLTNGDVTT
                     LQNTNVLNFSKDDLFAGQDLSVTLGHEMYSRSSENTTSDIQAFPVDYDSFMAWDDIAS
                     GTNRYGDAFFRDMDDRMISFFGRVNYNILDRYLLAATFRTDGSSKFSGDNKWGYFPS"
     gene            8730..13157
                     /locus_tag="PKH68_03050"
     CDS             8730..13157
                     /locus_tag="PKH68_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNX11946.1"
                     /translation="MIKRLFLLISGVLMSLSPLIAEPMCHIRHYSTEDGLPQYNIMDM
                     LQDRKGYLWLATWDGLSKFDGYNFRNYKAKSGNKYFMKSNRIEKLYEDAYGRIWFKSY
                     DGDTHCFNPSNEEFWGLQSMDNTTNDKISNIEIKPSGKIWLIVEKNGCMLLKDSLFKP
                     NIFNLKNNKLSSERVNSILEEKSGNTWILTDNGIVSLSTNNLTKKYFSEPISTRKENY
                     QAFFNGIELNNEIWFGSQNGRIWRINKKSGKAGLLQLQVQSDIIDFIPVSEFEVGIFT
                     RNAGFAIYNTHTNTVAFFNTGNLNGLSSNEIKPVYVDKVHNLWFETNTIGINRFNLQS
                     HSFKSYRVSTIDMMSFVSPPVTLVIEDKNGRLWVQPKGGGFSLYNSTTDALEPFFNSP
                     TAENWRFSSTIYSAYSDLQGNLWLSTHSHGLEKIVFDNSYFTATSISSHKFPKTSNEV
                     RAVFEDNDLNLWISTKDRKLTVFDKNKRKLGELDADGNIRSSASMPAIAYSIIKDKND
                     DIWVGTKGAGIFILKKTANPLKFRIEHYSHSSADVYSLSDNSVYSIFEDHQGRMWIGT
                     YGGGLNLVNKSNDGKLTFINHRNQLKNYPIDVCAQVRFVTQNMGGNLCLGTTSGMLMF
                     SPSFSSPENITFKRYTVEPGSSNCITNNDVHGICITRKGEMFLFTFGGGINKVTKFDK
                     QGFPLDFKSYTRSNGLSSDVTLAMVEDEFGKLWISTENSLTKFDPEKEVFKTFSEIRK
                     LMISSNFSEVSTCRLRNNDIIFGYSDGYLSFSPRNIVRSNFKPYIAFTNLQLFNKDVS
                     IGEKSPLKSIVDDIPKLKLTHKQKFFNIEYAALDYLDPENILYAYKLDGFDDDWNYVQ
                     KQRIAYYTNIPKGKYLFRVKSTNSDGDWVDNERTMAIEVMPSFWQTGFARLLYILLAV
                     GLLYLFFRIILSFYHLKANVALEKKMSEMKLRFFTDISHEIRTPLTMITGPVDYLLHD
                     SKTPDDIKHQLHVVSQNSNRLLRLVNQILDLRKIQFQNLKVQEVELANLVSKICDNFT
                     DIAQNQQIQFNYQDLTRNATVWVDPDATEKIVMNLLSNAFKYTPAGKSIDVIIREDEK
                     NLMLEIKDQGVGISKEKQKKLFTRFVTFSDDKIKPSTGIGLNMIKELADKHAAKVSLE
                     SEEGIGSSFTVSFQKGLAHFGKDVEIIAASNENLIKNEVKEENGNAVETECPPLAKNQ
                     KQSILIVEDDDDLRAFIRTILEEEYQVLEAADGEAGMKMALKQLPDFIVSDIMMPNMD
                     GVELLQALRREKTTCHIPIVLLTAKTTIETKLEGLQYGADDYITKPFSVSYFRARIAN
                     LLQQRKDLQRIYRENLVPAAQPGTGNTVAAVDYQPQTVTIASHDHEMMKKIMTLIENN
                     MDNTEFSVEEMGQYVGMSRSVFFKKLKSLTGLSPLEFVRDIKMKRAAQLLDTGELMVK
                     EVASQIGITDTRYFAKCFKAKYGMIPQEYKSTRYKKKEE"
     gene            complement(13225..14004)
                     /locus_tag="PKH68_03055"
     CDS             complement(13225..14004)
                     /locus_tag="PKH68_03055"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HNX11947.1"
                     /translation="MVNFSLEGKIALVTGASYGIGFALASAFAQAGATIVFNDINQEL
                     VNKGLAAYKEAGIKAHGYVCDVTNEDQVNTFAALVEVEVGVVDILVNNAGIIKRIPAL
                     EMTAAEFRQVIDIDLNGPFIVSKAFVPGMIKKGHGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNFASEWGEFNIQCNGLGPGYIETPQTAPIRVPGHPFNEFIIAKTPAGRW
                     GKTEDLEGPAVFLASDASNFVNGHILYVDGGILAYIGKQPK"
     gene            complement(14024..14866)
                     /gene="kduI"
                     /locus_tag="PKH68_03060"
     CDS             complement(14024..14866)
                     /gene="kduI"
                     /locus_tag="PKH68_03060"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HNX11948.1"
                     /translation="MKTNYELRYAAHPEDAKQYTTARLRKDFLIEKVFSSDEVNMVYS
                     MYDRMVVGGAMPVNESLKLEAIDPLKQPVFLMSRELGIYNVGGSGVVKVGNEKYELNY
                     KEALYLGSGNREVYFESNDAANPAKFYFNSATAHRNYPDKKVTKADAVTMELGSLETA
                     NHRVINKMLVNQVLPTCQLQMGMTELAPGSVWNTMPAHVHSRRMEAYFYFEVPADQSV
                     CHFMGEPDETRHIWMHNDQAVLSPEWSIHSAAATYNYTFIWGMAGENLDYGDQDFSKI
                     TDLK"
     gene            complement(14913..16340)
                     /locus_tag="PKH68_03065"
     CDS             complement(14913..16340)
                     /locus_tag="PKH68_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX11949.1"
                     /translation="MDNTQKMTNFRWTIVVLLFATITINYLDRQVLSLTWDEFIKPEF
                     HFGDDIYGFITGFFSLFYAVCYLFAGRFVDWIGTKKGFLWAIGIWSLGACVHAVCGIA
                     TEAVVGLPDKTALINATGDVAVVISTVAMWAFIVARSILALGESGNFPSAIKTTAEYF
                     PKKDRAYATSIWNSGASVGALASPLAIPPLAKAFGWEMAFIIIGALGFIWMGFWVFLY
                     DSPEKNKRVNAAELEYIDQDKHEQVSEKAEKIEKIPFLKCFTYKQTWSFAFGKFMTDG
                     VWWFYLFWMPLYIKAQFDIKTTDPTGQLLIFTMYAIITVLSIYGGKLPTIFINRSKQN
                     PYAARMKAMLIFAFIPLLVLVAQPLGTYSYWFPVIIIALAGSAHQSWSANLFSTIGDM
                     FPKGAIATITGIGGMAGGLGSFFLQNTAGKLFIHAEKTNLQFLGFEGKPAGYFIMFCF
                     CAVAYLIGWTVMKSLVPKYKVITDF"
     gene            complement(16367..17035)
                     /locus_tag="PKH68_03070"
     CDS             complement(16367..17035)
                     /locus_tag="PKH68_03070"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNX11950.1"
                     /translation="MMKFNKIQVLTVMSETGIIPVFYHKDGEVAKKVLKACYEGGIRA
                     FEFTNRGDFAQEVFAGLVKFAAVECPEMIVGIGSIVDAPTAAMYLQLGANFVVGPLFN
                     PDVAKVCNRRLVPYAPGCGSVSEVGFAQEAGCDVCKVFPGDVLGTGFVKGLKAPMPWS
                     MLMVTGGVKPEETNLRGWFDAGVTCVGMGSNLFPAEMIKAGDWEGITKLCSDALAIVK
                     KIKK"
     gene            complement(17195..18235)
                     /locus_tag="PKH68_03075"
     CDS             complement(17195..18235)
                     /locus_tag="PKH68_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNX11951.1"
                     /translation="MSKVVTFGEIMLRLATPGFQRFIQSASLNATFGGGEANVAVSLA
                     NYGIDTEFVTRLPQNDIADWCVSELRKYNVGTKEIIRGGERVGIYFLETGAVSRSSKV
                     VYDRANSSIADIKPGMINWHEVLKDADWFHWTGITPALSQGAADACLEAIKVANELGV
                     TVSTDLNYRKNLWKYGKKASEVMPELVAGCDIILGNEEDAEKVFGIKPEGFDAEHTGG
                     EVDAAEFQSVCAQLMNKFPGAKKVIITLRGSINANHNTWGGVLYSNDTLHQSKRYDIT
                     HIVDRVGGGDSFMGGLIYGLLTYTTDDQKALDFAVAASCLKHTIYGDFNLVTVSEVEN
                     LMKGDGSGRVMR"
BASE COUNT         5302 a         4068 c         3736 g         5174 t
ORIGIN      
        1 tcagcctctt atttatattt ttttcccgag atgcttatat tgctgttgcc attttgtttc
       61 aagtcaatgg gtttctcaag tcctgatgga taagtaaaat tttctgaaat aaaattttcc
      121 acatctttca aaattaatgc tgaaccgttt tgaggaacga tatgtacatt tttgaaaaca
      181 atatcttttg aattacttaa ctctgcgcca tattgagcag taatattaat atcttcaatc
      241 tgaatgttat ttattttcat ttcgggcaat ccattgaata acatggctct gcgggcatta
      301 cgcgaaacaa gattcttgac gaatatattc ctgaataccg gagtttcctc ggttacgggc
      361 agtagttttt cttctgttgg agttacgtca ccatcttcaa gggtttctac agccgatttt
      421 ccgccgtaat agaggtcaaa aaggaatgat tcggtttcga tattaaacat gtagatatct
      481 gaaatgtaaa tattttcgac cacaccgccg cgtccgcggg tacttttgaa acgcagacct
      541 acatcggtgc ccagaaactg gcaatttttc acagagatat tgcgcacccc gcctgacatt
      601 tcactgccaa ctacaaagcc accgtgtccc tgaaacacct tgcagttatc cacaatcaca
      661 ttttcggtgg gacgggcacg ccgcctacca ggctcgtcct tgccggattt gatacaaatt
      721 ccgtcatcac ccacatcaaa ggtgcagttg actatgatgg aattgcgaca agactccaga
      781 tccagtccat cgccattctg tgaataaccc ggatttcgga caaacaatcc atcgagaata
      841 acattttcgc acatgagcgg gtgcaaattc cagctgggtg aattttcaaa aagtacgccc
      901 tggagataca catttttgca ttcgatgaaa ctgaccataa cagggcggag aaaatccttg
      961 atttcgagcc attcctgttc ggtcagattt tggcgcggca cattcatatc gcttatctgg
     1021 gaacccttaa aagagctttc tgacggatac caggtatcat tatttttaac cacacctcct
     1081 gctccgatca tttttttcca ctgactggct gtgactttat tctttttgag cgggcgccag
     1141 gcttcaccgt aaccattgat gcttccctga cctgtaatgg ctacattcac caggttacga
     1201 cccgaaatgg gcgattgaca acgccgcgta tcgagccctt cgaatatggt ttctacaatg
     1261 ggatagaggt tgaagtcggg tgaaaacagg ataacggcac ccttctccaa atgcatattg
     1321 atattggagc gaaatacaat gggtccggta aaccaaatgc ccttaggaac tatcaatgtg
     1381 ccaccacctt tggctgccag ggcgtccatt gatttagcaa atgcattggt attgagagta
     1441 ataccatcgc ccaccccacc aaattcttcg actgaaacca tatttttttt aaataccggt
     1501 actttgacca acggcattga aaatggtaaa tccttataaa gatattgata gtggtttttg
     1561 gcttcagcat taaacccaat gtttagcacc atgtaaacca gtatcaaatt tcgcatttta
     1621 tatagtattc tcatattaag tttgttttat gataataaaa acaaatactt tattcacaaa
     1681 aatctcacac agtattattg gagggatatt tatctgaata ccagataaat atcccatatt
     1741 tcacataaac actaccaacg caaatcgcct gctacgcctt ttccgccaaa agaagtatcg
     1801 agtagtttga aattgccgtt tacaggatct gtccacaatg ccgtagatgc acttttatag
     1861 gaggtcatat tgggtttgat agaagtgctc gtagctccgg ccggtatcgg atcgtccaca
     1921 taatcagtag taaagtatga gccctttatg gaaacagatg ttccggccac atatctaatt
     1981 ccatttgcac ccatagcggc accggtctga ccgaatacac aattgctgat agtaactcca
     2041 cctcccgtga aagaagcatt gttgaaatcc atcaaatacc gggctgaacc tgcgtcttgc
     2101 atgccgtgat caatgttaca gttgataact ttcaccgatt tcagggtttg acccgtgcgc
     2161 agcaccagtg agcctttgaa attatagatg gtactgttcg ctatttcaat ggcgttgata
     2221 aggtctgccg aattactgtt gacaatggca taagtgcttg aaaatccgat atcattaatc
     2281 ttacaaccat tgatcgacaa actgtcaatt acctgatttt tagcaccctg aacacgcatg
     2341 ggtgtattgc caaagttgtg catattgcaa ttggcaaacg aaagtttgga aacagtagtc
     2401 atcgtattgt tattaaacag atatccgatt ttagtggctg ccgtattatt atcaacatat
     2461 cctgtaaaat caatattttc aaacttaact gattgcatgg acgaaccatc cacaaatcca
     2521 agcatactag aagttgctga gggaggtgta ccggtgccca cagtcataca aactaccgga
     2581 agagttgatg tcagtgttcc tctcagctta accgatttat tgaaccgata cgttccgcta
     2641 cctacattat acaccgctcc gccttccaaa actattacct gtccatcagc agcactgttg
     2701 agcgctgctt gcagatcttc accggatctg accaaaatat ccccttccac tgtaccatag
     2761 gttgttcctc ttaatatgga attgttaaaa atccggactt tccataccga attggacaat
     2821 gaagaaacaa ccttgcttcc ggcaagtgct tcggcagccg atatcaaaac cgaatctttt
     2881 gctgtggcat tttcattggt taaaattaaa tgggtaacat tggcagctgg tgtccatttt
     2941 acggttaatg cattgaaatc ggtcatatat gcactgtaaa ccacaccggt attaatactg
     3001 gttccgtacc cctggaatag attttccttt ggagttttga aagaaagtgc cgtattgaat
     3061 ttggatgatt tagcagcatc agcggcattg gcacggatac gggcataaaa tttggtatcg
     3121 cccgccaatt ctttgaccaa cgacaaaccg gtagtggtta catcgagcaa aggcttggag
     3181 taatccagac tatcgttgct gagctggagt gtgtacgaca cagcgctgtc taccttagcc
     3241 catgaaaaag tggcaacggt tttattcagt tcaacattaa aattgattgg ccggaacatg
     3301 tatggtaatt gcagttctgg ttcttcttcc ttgcaggcgg agaaaacgcc cacaatcatc
     3361 agaagaatta gaatagttcc gtatgttctg tttttatatt ttttcattgt tttaattact
     3421 taataggttt ttattctgca atttacatta attatcatac ccataatcgt tgacgagcga
     3481 gccgttgctg gcaagaataa tctggtgcat gatgggcaat agctgacgtt tgtcgggatt
     3541 gcccagatac atgttattga taaatccgtc gctcaggtaa agttcacccg tagaggttgt
     3601 tgcgtttgtc caggtttttt tggtccatcc gccggtgggg tcggttaccg ttttgtcatc
     3661 ggtttcacca cgttgcaggc cataaactat gagtacccgt tcaccggttg taatactgtt
     3721 gtccaccaca tatttgtaat aaatgacaga aggaactgta gcatagtcgc cggtgccgtc
     3781 gcgcagtgca ataagactgg ctttggcttt atcgatggat gctttgagtt caccccatcg
     3841 ttccagatca tacttgcgga tgttttcacc gcaaaattca aatgcccgtt ccattttgat
     3901 attggcgagt gtgagtggca aatcgtaagt ggctgccgta gttccgaatg cacggcggcg
     3961 aacctgattg aaataaccgg caccttcaga gccaccgagg aataaatcgg cttcggcaaa
     4021 catcagcaat acatcggcat aacgcaatac tatcggtgaa acaccatcgt cagttccgga
     4081 aataggaatt ttaacccatt cggcacgcca ttttcccaga ttgaaaccgg tcacaccact
     4141 cgaaagtgat tgttccatca ctcccgtggt tgcatttttt accactttga atggaactac
     4201 cgtgatatcg cggcgtttgt cattcacgtt ataatcgtag taaaaatttg gcataattct
     4261 gaactgcgga cttaatgcca ctttgttgtt tttaccatcg gcttcgcgcg ataatcccaa
     4321 attataaacc atacggccac gaatacccag attgtaaggc aattcccaga ttgcttcgcg
     4381 tccgtgagtt tccacatcct gacattgttc gtagaaaata tttttaaaat ccgggtcaag
     4441 tttataatgt ccgtcagcaa tgatagcgcg acatgcatcg cgtgctatgg tgtagagttc
     4501 gcggcggcga ctgtccgatg cggttatttt tatcacggca tcggtgcccg acgggcgctg
     4561 agagtatcct gcagccgaca agcaaattcg ggccagtaat ccgcgggctg catccctgct
     4621 gatcatttta acggtattgg tatatttcgt tcccggagcg taaagcagtg tcgatgcttc
     4681 ctgcaggtct ttgattatct gattgtaaat ggtatcccgg tcggttttgg caagatagat
     4741 agtagcttcg cttaccggtt caaaacgcgg aggtacatcg ccccaccatt tgatgaggtc
     4801 gtagaaaaaa taggctctca tagtaagtgc agaaccgagc agatgaccca tatcattgcc
     4861 ttcggcagga tttccaaatt tgcggatacc ctcaatagcc agatttgccc gctcaatgcc
     4921 ctgatagata cggctccagg gattggcagc atcggtattg ttgtaaccgt cacctatcga
     4981 tgaatttgga ttgtaaagag caaagaggtc gtcggatttg atggaggaga gtacagctcc
     5041 cgatgaccat cccgaacgaa attcaatgtc ggtattcaca cccatctgta accacagacg
     5101 gtttcggtac gaattgtttt cgccaatgat gctataaatg gcactgatag ctttataggc
     5161 agagtctggc tgactgaata tcacatcatc gctgatgagc gaattgctcg gcacatccag
     5221 aaagctctcg caggaagaga atgatagcat cagcgctgca attcctgcca atatatttat
     5281 tttacttagt cttttcattt ttattggatt ttagaaggtt aagttgatgc caaagttata
     5341 cgaacggctt cttggatatg ctgaataatc cacacccggc gtacacggtg tagcacggcg
     5401 tgtatccact tcgggatcgt aacccgagta attggtaaag gtgtaaatgt tgtaagccgt
     5461 tgcataaagt cgcagtttct gtatgtacag tcctttcaat aatcgggttg gtaaagtgta
     5521 tcccactgtc atattattga accgaaggaa tgagccgtct tcgattgccc aggatgtgat
     5581 ggcatagcgg ccggtaggtg gcgcccagat ggaagcatga ctgttggcat tcatcagatc
     5641 gagttcattg acatctgtaa tccgtttgcc ggtttcccag ttcatggtag tatatgaatt
     5701 cagattgctc gaaagcatat tgcggtattt gtaatagaat gatgacattt caattttgtt
     5761 ggcgttataa atgtcattgc catacaccca gttgaaattg agggcaaaat cgacccccct
     5821 gaaatcgccc gaaaatgcaa atgaaccgaa atgtgcggga ttggtattgc caatcactac
     5881 gcggtcgagg tcggatatct ttccatcgtc attctgatct ttcagtttca tggcaccggg
     5941 cccccagctt agtccatcga tagagctgtt gtccacaaat ttatttccat actgatcgga
     6001 ataaattttg gttgctgcat cgtacttatt gtattttccc gaattcatga cccatttggt
     6061 gccattccac ataaaatctt tggcactgta ccatccctca ttcacatatc cataaatctg
     6121 acccacaggt tcaccgggtt taaccacata gctgttggat gcttcagtca tactggtcca
     6181 ggcttcgttg aacgaatacg aatccagtcc gcccaaatca tccactttat tttgattggc
     6241 actgatattg aacatgaaat tcagattgaa gtttttactt tcaaccagac caatattcaa
     6301 tgacagctcg gctccgcggt tggaagtgga agctgcattt tgccactgat aggtatagcc
     6361 tacaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaatg aagggaaata
     6481 accccatttg ttgtcgccgg aaaatttact ggaaccatcg gttcggaaag tggctgccaa
     6541 cagataacga tccagaatat tataatttac ccgaccgaaa aaggatatca tgcggtcatc
     6601 catatcccgg aagaaagcat caccgtatcg gtttgtgccc gatgcaatat cgtcccaggc
     6661 catgaatgaa tcataatcaa ccgggaatgc ctgaatgtcg ctggtggtat tttcgctcga
     6721 ccggctgtac atttcgtgac ccagtgtaac agataaatcc tgtcctgcga agagatcgtc
     6781 tttggagaaa ttcagcacat tggtattttg cagcgtagtt acatcgccat ttgttaataa
     6841 tatggatggt gccacagcat tatcacgttt cagcgctccg ccttcacggg caaagtacga
     6901 agttacaccg tagaaacgtt tgtcgtcctt attgcttttg gttaatccat acgtacttct
     6961 aagagaaaga tttttggtaa aattcactgt aaatcctccg ttgatattgt aatccctctt
     7021 tttctgatac cggttgttgt cggctgtggt tacaagtgga gagtacagac ttgcaacagc
     7081 atccatatca tcgtcctgtg ccaccatatt ttttaattca atgggagtat aaattacggc
     7141 atttttcaca cgcgaatcgg aagtggattt ttcggatccg gtcacatcat tagcgcctga
     7201 gccattaatt actgtagcag cgtagcgtgc agccacatcc agtttcagcc atttgagcgg
     7261 ttgataattc agttttccac tgaaattgtt gcgattataa tcggacatgg ccatgatggc
     7321 tttgtcagac aaattggtgt agctgaaatt gtatttcaac tgctttgtac cacccgataa
     7381 tgctaccgaa tgattttgct gaaaaccggt acgaccaaat gtttcgtcct gccagttggt
     7441 tccgttttta tagttatata aattaatgtc ttcgtagctg ccaaagtatt tttcatattc
     7501 tgacgctacg gcattattcc aggtagcccg ttcgtattgc ttttgtacaa actgatacgg
     7561 actaagcact tcaagttttt tggtaatgtt tttaaaaccg acatatccgt catacgaaag
     7621 ggaaattttt ccctgtttgg ccgatttggt ggtaatgatg attaccccgt tggcaccccg
     7681 ggaaccataa atggctgtgg aagaggcatc tttgagtaca tcgatggact gtatttcgga
     7741 aggagccaca tccttgatat cgtctttgag aaatccatct acaatataaa gtggtgaatt
     7801 gctttgggtg atagaaccgc ctccacgtac acggatcttt atttcggcat cgggcgaacc
     7861 ttcagttgta gtcacctgca cacccggcag ttttccggtc aatgcttcgg ctacggtagc
     7921 taccggtatg tctttcaatg ctttttcgct gacagacgat atggaaccgg tcaaatcccg
     7981 tttacgctgt gtgccatacc cgatgaccac cacttcgtcg agagttttca gcagatcttc
     8041 gagcaccaca ttgacaacag atccggcaat atcaacttcc ttattgattt ttcccacgga
     8101 gcggaatatc aatgttctgg ctgttgcagg tacgcccagg gtgtattttc catccacatt
     8161 ggtgaccgtt ccctgagtgc cttcttttac aattactgta gcgccgatta caggatcacc
     8221 gttctgatct gttactgttc cggaaatggt acgttgttga gcaacaatat tcgtactcag
     8281 cagcaacatt ccaagcatca gcgcaatgtg tctaactctg attttgtgtt tttgcatatt
     8341 gatttgagaa ttaattgaaa ttaataattt ttattgtgta attattggca ttgattgtgg
     8401 tatgaaaata taaaccacat ttttgaatgt caatcaagag ttaatggtta atttttaatt
     8461 attttaaggt ataatatcga atgcaattat agttatattt gaattaaacg aggtgtgtca
     8521 tttcactttt tgaagtggga taaattaatt attaagaaaa ataaatcgtt tcggtggcct
     8581 gatttagttc acaaaccgaa taaaaggcaa ttacgaaatt gataatttac tcaaaattag
     8641 tatttttgtc taatatgatt tttataaaac ctattactat agaaatttaa cagacaaaac
     8701 ctcagataac gttatttaaa caattatcga tgataaaaag attatttctg ctaatttccg
     8761 gagtgttaat gagcctctca ccgctcattg cagaacccat gtgtcatatc aggcactatt
     8821 ctaccgagga tggacttcca caatataaca ttatggatat gcttcaggac cgaaaaggat
     8881 atctgtggct ggctacatgg gacggattga gcaaattcga cggttataac ttccggaatt
     8941 ataaggcaaa atcgggcaat aaatacttca tgaaaagcaa ccggatagaa aaactctacg
     9001 aggatgctta cggcagaatt tggttcaagt cttatgatgg tgatacacat tgctttaatc
     9061 cgtccaatga agagttctgg ggtttacaat ctatggacaa taccacaaat gacaaaatat
     9121 cgaatattga aatcaaacct tcaggtaaaa tatggcttat tgtagaaaaa aacggttgta
     9181 tgctgctgaa agattcactg ttcaaaccca acatatttaa tctgaaaaat aataagttaa
     9241 gttccgaaag ggtgaatagt attctggagg aaaaaagcgg caatacctgg atacttaccg
     9301 acaatggcat tgtatcactt agcaccaata atttgactaa aaaatacttc agcgaaccga
     9361 tttcgacccg aaaggaaaat tatcaggcat ttttcaatgg aatagaactc aacaatgaaa
     9421 tctggtttgg ttcgcagaac ggccgtatct ggcgtataaa caaaaagagc ggaaaagccg
     9481 gtctattgca gcttcaggtg caatcagaca ttattgactt tattcctgta tccgaattcg
     9541 aagtcggcat tttcacccga aatgccggat ttgccattta taatacacat acaaatacag
     9601 ttgcattttt caacaccggc aacctcaacg gcctgtcgtc caatgaaata aaacctgtat
     9661 atgttgataa agtacacaat ctgtggtttg agaccaatac gatcggcatc aaccggttca
     9721 atcttcaatc acacagcttc aaatcgtatc gggtttccac cattgatatg atgtcatttg
     9781 tttcgccacc cgtcacactg gttatcgaag acaaaaacgg aagactatgg gttcagccca
     9841 aaggtggcgg attttcactg tacaatagca ccacggatgc gctggaacca ttcttcaact
     9901 caccaaccgc cgaaaactgg cgattttcga gcaccatcta ctccgcctat tccgatttgc
     9961 agggcaatct gtggctcagc acacactcac acggactgga aaaaatagtg tttgacaaca
    10021 gctacttcac ggctacatcc atttcgagcc ataaatttcc gaaaacatcc aacgaggtca
    10081 gggctgtatt cgaagacaat gacctcaacc tctggatttc gaccaaagac cgaaaactta
    10141 ccgtatttga taaaaacaaa cgaaaactgg gggaactgga tgccgacgga aatatccgct
    10201 cctccgcttc gatgcccgca atagcttata gcattatcaa agataaaaat gacgacatct
    10261 gggtaggaac aaaaggtgcc ggcatattta ttctcaaaaa aacagctaat ccactgaaat
    10321 tcagaataga acattacagt catagtagtg cagatgtgta cagcctgagc gacaacagcg
    10381 tgtacagtat ttttgaagac catcagggac gtatgtggat aggaacttac ggaggcggac
    10441 tgaacctggt gaataaatca aacgatggaa aactcacttt tatcaatcac cgcaatcaac
    10501 tgaaaaatta tcccatcgat gtatgcgctc aggtgcgatt cgtgacacaa aacatgggcg
    10561 gaaacctttg cctgggcact acaagcggca tgttgatgtt ttctcccagt ttttcttcac
    10621 ccgaaaatat aactttcaaa cgctacaccg ttgagcccgg cagcagtaac tgcatcacca
    10681 acaacgatgt gcatggtatc tgcatcaccc gaaagggcga aatgttcctc ttcacatttg
    10741 gcggtggtat caataaggtt accaaattcg acaaacaggg ttttccgctg gatttcaaat
    10801 cctacacccg ctccaacggc ttgtcatcgg atgttacact ggccatggtg gaagatgaat
    10861 tcgggaaact gtggataagc actgaaaaca gtctcaccaa atttgaccct gaaaaagagg
    10921 tttttaaaac attttccgaa attcgcaaac taatgattag cagcaatttt tcggaagtca
    10981 gtacctgccg gttgagaaat aatgatatca ttttcgggta ctccgatgga tatttgtcat
    11041 tttcgccccg aaatattgtc aggagtaatt tcaaaccata catagcattc accaacctgc
    11101 agctattcaa caaggatgtg tcgattggtg agaaatcgcc gcttaaatcc attgtcgatg
    11161 acattccaaa gctaaaactg acccataaac agaaattttt taatattgag tacgccgctc
    11221 tggattatct tgatcccgaa aatattctgt atgcctataa gctcgatgga tttgacgacg
    11281 actggaacta tgtgcaaaaa cagcggattg cctactatac aaacattccg aaaggaaaat
    11341 atctgttcag ggtaaaatcg accaacagcg atggcgactg ggtggataat gaacgaacaa
    11401 tggcgattga agtcatgccg tcattctggc aaacaggatt tgcgcgattg ctatacattc
    11461 ttctcgcagt tggacttctg taccttttct tcaggataat actgtcattt tatcacctca
    11521 aagccaatgt ggcgctggag aaaaaaatgt cggaaatgaa actgaggttc tttaccgaca
    11581 tatcgcacga aatccgtaca ccgcttacca tgataaccgg tccggtggat tacctgcttc
    11641 acgacagtaa aacccccgat gatatcaaac atcagttaca tgtcgtttcg cagaactcaa
    11701 accgcttgct gaggctggtc aatcagattt tggatttgcg aaaaatacaa tttcagaatt
    11761 tgaaagttca ggaagtggaa ctggccaatc tggtatcaaa aatctgtgac aactttaccg
    11821 acattgcgca aaatcagcaa attcaattta actatcagga ccttacccgc aatgcgactg
    11881 tgtgggttga tcccgatgct accgaaaaaa tagtgatgaa cttactgtcc aatgcattca
    11941 aatacactcc tgcaggcaag tctatcgatg taattatccg agaggacgaa aaaaacctga
    12001 tgctggaaat taaagatcag ggtgtgggaa tatcgaaaga aaagcaaaag aaacttttca
    12061 cccgttttgt aactttcagt gacgataaaa tcaaaccaag cacaggcatt ggactgaata
    12121 tgatcaagga actggctgac aaacatgcag caaaagtaag tctggaaagc gaagaaggca
    12181 ttggaagctc atttacggtc agtttccaga aaggactcgc ccactttgga aaagacgtag
    12241 agataattgc tgcctccaac gaaaatctta ttaaaaatga agtaaaagaa gaaaatggta
    12301 atgctgtaga aacagaatgt ccaccattgg ctaaaaacca aaaacaatcc atactgatag
    12361 tggaagatga tgatgacctt cgggctttca tccgcaccat tctggaagaa gaatatcagg
    12421 tgctcgaagc cgcagacgga gaagctggta tgaaaatggc actcaaacaa cttcctgact
    12481 ttatagtgag cgacattatg atgccaaaca tggacggtgt ggaactttta caggcattac
    12541 gtcgtgaaaa aacgacctgc cacattccaa tagtgcttct cacagccaaa acaaccatag
    12601 aaaccaaact ggaagggttg cagtacggag ccgacgatta catcaccaaa ccgttcagcg
    12661 tctcttattt ccgggccaga atagccaacc tgctgcaaca acgcaaagat ttacagcgca
    12721 tataccgcga aaatcttgtt ccggctgctc aaccgggcac aggcaatact gttgctgctg
    12781 tcgattatca gcctcagacg gtgaccattg cttcgcatga ccatgaaatg atgaaaaaaa
    12841 taatgacact gatcgaaaac aatatggaca acaccgaatt ttcggttgaa gaaatgggac
    12901 aatatgtagg aatgagccga tcggtatttt tcaaaaagct taaaagcctg accggactgt
    12961 cgccgcttga atttgtacgg gatataaaaa tgaagcgggc agcacaattg ctcgacacgg
    13021 gcgagttgat ggtaaaggaa gttgcatcac aaattggcat tacagacacc cgctactttg
    13081 ccaaatgttt taaggccaaa tacggcatga taccacagga atacaaatca accagataca
    13141 aaaagaaaga ggaataagag aaaaaaaaac ggtcaggaat gaatttccca accgtttttt
    13201 tttagtttca taaattgcat gttgtcattt tggttgttta ccgatataag ccaaaattcc
    13261 gccatccaca tacagaatat gtccattgac aaagttggat gcatccgaag cgaggaatac
    13321 ggcaggccct tcgaggtctt cagtttttcc ccatcggccg gccggtgttt tggcaataat
    13381 aaattcattg aacggatgac ccggaacccg gataggtgca gtctgcggag tttcgatata
    13441 accagggccc agaccattgc actgtatgtt gaattcgccc cattccgaag caaaattctt
    13501 ggtcagcatt ttcagtccgc ctttggctgc tgcgtaggca ctcaccgttt cacgtccgag
    13561 ttcactcatc atcgagcaga tattgataat tttgccatgt ccttttttga tcatacccgg
    13621 tacaaacgcc tttgacacaa taaacggtcc attaaggtcg atatcaatca cctgccgaaa
    13681 ttcggctgca gtcatttcca atgccggaat tcgtttgatg ataccggcat tattaaccag
    13741 aatatccact actcccactt ccacttccac caatgccgca aatgtattta cctgatcttc
    13801 attggttaca tcgcacacat atccgtgagc tttaattcct gcttccttat atgctgcaag
    13861 ccctttattc accagctcct ggttgatatc attgaaaacg atggtagcac ccgcctgagc
    13921 aaaagccgaa gcaagtgcaa aacctatccc gtaggaagca cctgtaacca gtgctatttt
    13981 tccttctaat gaaaaattta ccatattatt gtttattagt taattatttc aaatcagtta
    14041 ttttcgaaaa atcctgatcg ccataatcga gattttctcc ggccattccc cagataaagg
    14101 tataattgta ggtggctgca gctgagtgaa ttgaccattc gggtgaaagt accgcctgat
    14161 cgttgtgcat ccagatgtga cgggtttcgt ccggttcgcc cataaagtgg cacacagact
    14221 ggtctgcggg cacttcaaaa tagaaatagg cttccatacg gcgtgagtgt acatgcgcag
    14281 gcatggtatt ccacacactg cccggagcaa gttcggtcat tcccatttgc aactgacagg
    14341 taggcagtac ctgattgaca agcatcttat ttattacacg gtgattggca gtttcgagcg
    14401 aaccaagttc catagtaaca gcgtcggctt tggtaacctt tttatcggga taatttcggt
    14461 gagcagtggc cgaattgaaa taaaattttg ccggatttgc agcatcattg ctttcaaaat
    14521 atacttcccg gttgcctgag cccaggtaaa gcgcttcctt gtaattcagt tcatactttt
    14581 cattaccgac ctttactacc ccactaccgc ctacgttata aattccaagt tccctgctca
    14641 ttaaaaaaac aggttgtttc agcggatcga tagcttcgag tttcagactt tcgtttacgg
    14701 gcattgcacc gcccaccacc attcgatcgt acatggaata aaccatattc acttcgtctg
    14761 aagaaaatac tttttcaatt aaaaaatctt tacgaagtct tgctgtagta tattgctttg
    14821 catcctcggg atgcgcagca tatcttaatt cataatttgt tttcatacaa ttcaatttga
    14881 aaattactga tttgttaatt cagtttctgt tgttaaaaat ccgtaatcac cttatactta
    14941 ggcaccagcg atttcatcac cgtccaaccg ataaggtatg ctaccgcaca aaaacagaac
    15001 ataatgaaat atccggcagg tttgccttca aagcctaaaa actgtaaatt agttttctct
    15061 gcatggataa aaagtttacc ggctgtgttt tgcaggaaaa aggaacctaa tccgccagcc
    15121 atacctccaa ttccggtgat tgttgctatt gcgccttttg ggaacatatc gccaatggta
    15181 gaaaacaggt tggcactcca ggattgatga gcagatccgg ctaaagcgat aattataacc
    15241 ggaaaccaat acgaataggt acccaatggt tgtgctacaa gtacaagtaa gggtataaaa
    15301 gcaaaaatca acatcgcttt cattcgggca gcatacggat tttgtttgct gcggttgatg
    15361 aaaatagtcg gtaattttcc accgtaaatg gacagtacgg taattatcgc atacattgta
    15421 aaaatcaaca attgacctgt tgggtcggtc gttttgatat caaattgagc cttgatgtag
    15481 agtggcatcc aaaacaggta aaaccaccac acaccatcgg tcatgaattt tccgaaagcg
    15541 aaagaccacg tctgtttata tgtaaaacat ttgaggaaag gaattttttc aattttctcg
    15601 gctttttcag aaacctgctc gtgtttatcc tgatcgatat attcgagctc ggcagcgtta
    15661 acgcgtttgt ttttttcggg actatcataa aggaaaaccc aaaatcccat ccagataaaa
    15721 cccaatgcac ctattataat aaatgccatt tcccatccga atgcctttgc taaaggtgga
    15781 atggccagtg gcgatgccaa tgcgccaact gatgcacctg aattccagat ggaagtagca
    15841 taggcgcggt ctttctttgg aaaatattcg gcagtggttt ttatggctga ggggaaattg
    15901 cccgattcgc ccaatgcaag gatacttcgg gctacgatga aagcccacat ggcaacggtt
    15961 gaaatcacaa ctgcaacgtc accggtggcg ttaatcagcg cagttttgtc gggcagacct
    16021 accacagctt cggtagcaat tccacaaaca gcgtgtacac aagcgcccag cgaccaaatt
    16081 ccgatagccc aaaggaaacc tttcttagtg ccaatccaat ctacaaagcg tccggcaaac
    16141 aaatagcata cggcataaaa cagagaaaaa aatcctgtaa tgaaaccgta aatatcatcg
    16201 ccaaaatgga attcgggttt gataaattcg tcccaggtaa gcgaaagtac ctgacggtcg
    16261 agataattga tggtaatggt tgcaaacagc aaaaccacta tggtccatcg gaagttcgtc
    16321 atcttttgag tgttatccat gatataattt taaaggttat tgaagattat ttttttattt
    16381 tttttacaat agcaagggca tcgctgcata gtttggttat tccctcccaa tcaccagctt
    16441 taatcatttc agccgggaaa agattggatc ccatgcccac acaggtaact ccggcatcga
    16501 accagcctct aagattggtt tcttcaggct ttacaccacc ggtaaccatc agcatagacc
    16561 agggcatagg tgctttcaat ccttttacaa aaccggttcc caatacatcg ccggggaaaa
    16621 ccttgcaaac atcacagcca gcttcctgag caaatcccac ttctgaaacc gaaccgcatc
    16681 cgggtgcata gggtactaaa cggcggttac acacttttgc tacatcggga ttgaagagcg
    16741 gaccaaccac aaaattggct cccaactgaa gatacatggc tgcagtcggt gcatcaacga
    16801 ttgaaccgat tccaactatc atttcagggc attctaccgc tgcaaattta accagtccgg
    16861 cgaaaacttc ctgagcaaaa tcgccccggt tggtaaactc aaaagcccgt atgccacctt
    16921 catagcatgc ttttagtact tttttagcaa cttcgccgtc tttgtgataa aataccggaa
    16981 tgataccggt ttctgacatt acagtcagta cctgaatttt attgaatttc ataatattgg
    17041 ccccctggcc ccctaaaggg ggaatttaga gcattgatta gagtatgtaa atatttcaaa
    17101 ttatgtaaga ataaacctat taattcccta aaaggtaaaa aaactctatg ttgctgtaca
    17161 aaaagttccc cctttagggg gttagggggc ccttctacct catcacccgt ccacttccgt
    17221 cacccttcat cagattctca acttctgata ctgtaacgag attgaaatcg ccataaatgg
    17281 tatgtttgag gcacgaagcg gcaactgcaa aatcaagcgc tttttgatcg tcggtagtat
    17341 aagtcagcaa tccgtaaatc aatccgccca taaatgaatc gccgccacct acgcggtcaa
    17401 caatatgtgt aatatcataa cgtttagact gatgcagcgt gtcgttactg taaagcactc
    17461 caccccaggt gttatgattg gcattgatag aacctcgcag ggtaataatt acttttttag
    17521 ctcctggaaa tttattcatc agctgagcac ataccgactg aaattcggca gcatctacct
    17581 cgccaccggt atgttctgca tcaaaacctt cgggttttat tccgaaaact ttctcagcat
    17641 cttcctcatt tcccaaaata atatcgcaac cggctaccag ttcaggcatc acctcggatg
    17701 ctttttttcc gtatttccag agatttttcc ggtaattcag gtcggttgaa acagttacac
    17761 ccagttcgtt ggccactttt atagcctcca gacaagcatc ggctgctccc tgcgacaatg
    17821 ccggagtaat tcctgtccag tgaaaccaat ctgcatcttt aagaacctca tgccagttga
    17881 tcatgccggg ttttatatca gcaatggatg aatttgcacg gtcgtatacc actttgctgc
    17941 tgcgtgaaac agctcctgtt tcgagaaaat aaatgcctac cctttcgccg cctctgatta
    18001 tttctttggt tcctacgttg tattttctca actcggaaac acaccagtca gctatatcat
    18061 tttgaggcaa acgtgttaca aattcggtat caatcccgta attagcaagt gacacagcca
    18121 cattggcctc accaccacca aaggttgcat tcagactggc ggattggata aatcgttgaa
    18181 atccgggtgt tgctaaccgc aacataattt caccgaaagt tactacttta ctcatttttt
    18241 atatttaaat attttgctaa taatcaatct atcaaagcaa tgaaaaatcc gttaaaattc
    18301 aaaaaaccgt ttggcattgt aataagaaat atcttccacc attttaccaa gaaagtctat
    18361 ctctgaagcc ggtaacagtc
//
LOCUS       DAOPZK010000029        18364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3181_length_18364_cov_24.050085, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000029 DAOPZK010000000
VERSION     DAOPZK010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18364
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_18364_cov_24.050085"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKH68_03080"
     CDS             complement(<1..544)
                     /locus_tag="PKH68_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNX11952.1"
                     /translation="MHHLLLIALVLLSGCGNKPGTGNEPPTVDITWQFYPESITLTSG
                     ASTQNLTVTSNGTWSVTSSQTWCTTSPTVGYNGQTSLKITAMNNSAEAERTAVLTFKS
                     GSYTKQFTVKQAAGTIQNYVPAGYTLKWQDEFNDARQTNGKTPLPSTTRWWYETGAGG
                     WGNNEIQNYISAYRGTDTCAV"
     gene            complement(697..>1695)
                     /locus_tag="PKH68_03085"
     CDS             complement(697..>1695)
                     /locus_tag="PKH68_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11953.1"
                     /translation="HMGLGPQGSFGQSWWGAGVNAKETWKLYDFKFTFIQAGVKLNIV
                     NKGEGYGRKASAATKGGFNVTTVDGDDALFAYNGGNYNFALDESGKYPKITLTGNAFM
                     GYYCGSQVYEIIYQTDKVMALRVDNTVEGQDWVFIYCLQELNVPAPPVVKEPKAVPLA
                     ENFETGTSGIKFVAEDMGSKSGIFDNPAPVPINESNKVYRYQKSTAFYSNLNFVAEKY
                     KFDLTKQNKIKVKVYIPSNDYTTDWATAGSWISNAKLRPQLAVKLQDSSLGGNAWQSQ
                     TEIVKADLALNTWIELTFDFSNVASRTDYDKIVIQFGAEGHAGPGLFFFDDFSFTE"
     assembly_gap    1696..1795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1796..3189)
                     /locus_tag="PKH68_03090"
     CDS             complement(<1796..3189)
                     /locus_tag="PKH68_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX11954.1"
                     /translation="MKKYIFIVFVALIGFTSCGEDFLTALPTEKQAAGGSATEGAILS
                     NLGSAYQILLFDSYANNNYNSIPLMSDLRSDDIYKGGGDAGDQHQLYLLSLFTTTPNE
                     NIDGLWSIFYTGLARCNNVIIACDNAVNVPDAKLNQYKAEAHFLRAYYVHWLWKFWGN
                     IPYFEAPLTTPPYMTKQNTANEVYAAIMKDLDFACGKSDGGTDRLPMNYAQGTPNSGR
                     VTKAAALMLKARVVMYQKDAARYGEVTKDMAEIIKSNDFDLMSNFGAMWLDENEFCKE
                     SIFESNQLPEGKTWSSGWQGYGTNLPAFISPNGLNTGSKTGDFKGGWGFGPVRQATWD
                     MYEEGDTRREGSINKWEASQYTARFQDTHLFMAKYSARVGYNPQGDVDLNYCNNFRIF
                     RFSETLLNYAEMVVVHGQSVVDGITAQSCLDRVRKRAFGMDKPIPATVANIKLERRRE
                     FVGEGIRFWDIVRWG"
     gene            complement(3211..6696)
                     /locus_tag="PKH68_03095"
     CDS             complement(3211..6696)
                     /locus_tag="PKH68_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX11955.1"
                     /translation="MRKQLFKRRTLILFTLMFIGLSSMGYAQKVSLNSSQQSLKQVLE
                     SISRQTGYTLAYSKEVVNLNEIVSINVRNASIQDVMAKLLTPRNLSYDIRDRKIFIAD
                     NSSGIESGQKAAQQADNVQIRGKVTDENGNPVMGASVAIPNSGLGTITNVNGDFTLSV
                     PRGAVLRVSYIGYTSQEFTVTSQSTLNVQLREDIASLDELVVIGYGVQKKSVVTAAIS
                     RVTADQLNNTKPSRVEDVLKGKVSGVQITQSSGQPGSDSKVRIRGIGTVNNSEPLYIV
                     DGMPVDGGINYLNPVDIQSVEILKDASSAAIYGARAANGVVLVTTKNGAIGKTQINYN
                     VSYGWQNPWKKKSVLNAQEYMVIMNEGLINDGGLPRYSNEQVMSGIEGTDWQDATFNY
                     DAPVKSHQLSVSGGTEKSQYFLSLGYFDQQGIVGGDYGKSNYNRWSVRSNSTHTIFEE
                     KSRDFLNELRIGVNLGYSRDKSSTIDANSEYGSILGSALTFSPLVPVYADEATAAAIL
                     AQYPNAVKDKDGRVFSIPPSGFQEIANPVGMLNQPSSGIGNSDKIVSTFWGELSLLPQ
                     LKFKSTYGVDLAFWGSDSYTFPYFLATQGKTVDVSTVQSEMNRGFKWQVENTLSYNQT
                     FANVHNVEVILGQSAQKYTYRNLGGNDQQLLETDPLKANINSAIADRKLERVWGGTGG
                     YNLASLASYFARLDYNFDERYILQATIRRDGSSNFGPTHKWATFPAFSLGWNLTNEAF
                     MQDRPKWMDALKLRFSWGKNGNERIGAFRYTSLMDGGQNYYFGGGYKVNPGDPTKVGE
                     TGGIMQYGSSPASIPNPDVKWEESQQTDVGFEAYLFKRAFTFSFDYFKKTTNGMLMEQ
                     PIPTYVGQGSPIANVGDMQNWGLEFETGWKQSIGDFKYFLSANASYLKNKLIKLGNAT
                     GEQIYENAGASGVGSYVKGKNGEVFPYFYGYKTNGLFQNQAEVDAYVNDKGEKYQSVA
                     KPGDVRFVDTNMDGIISDDDKVKIGKGMPDWTFGLTLGAEWKNFDLNVFFQGTQGNDI
                     FDFSQRGDIPAMNRPEWILQRWHGEGTSFTIPRMTIANANSNWRSSDLYIKDGSYVRM
                     KSTQLGYSLPGSLTRKASIEKLRLFIQAENLLTFTGYDGFDPEVASGGYTTIGIDRGI
                     YPQSRTISVGANISL"
     gene            complement(6948..7937)
                     /locus_tag="PKH68_03100"
     CDS             complement(6948..7937)
                     /locus_tag="PKH68_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNX11956.1"
                     /translation="MHPIDKETIDKVLNGTATKKEARQVAEWFSDTAEGQAYLSELMD
                     HEAAFIESNPDRYSDLSAVQSANMLAVINKRIRIKKTRRIYWQVAAALIPLILVVGLT
                     IRLNSQVNLFGKTVYSEVYVPKGKQEHIIFQDGSEAYLNSDTRLRYPRKFGLSKREIF
                     LQGEGYFNIATNKRRPFIVRINNTDVTVLGTSFNVHAFKEDNEMKILLDKGSIVFNTP
                     QNSYQILPGQQAVYDVLTGKCVIDYVDKPYEVSVWKDNIVLFRDTPLADVIKTLNRRY
                     NVEFAILNPKALTYTYTLTTNQTSLENIVKELEKIAPVKFSIRKDNSIIVSLK"
     gene            complement(8009..8572)
                     /locus_tag="PKH68_03105"
     CDS             complement(8009..8572)
                     /locus_tag="PKH68_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNX11957.1"
                     /translation="MNSNNEDSSLFLLIKKRDKEAFTVVYHKYHHYLYSLAFRYLKDV
                     ELAEDAVQHVFVKLWDSARYIDVDINMKNYLYTMTKNFVLNQIRDNKEMISLNYENAQ
                     IEITDESSDIVSIMEDKQLRSLLYEGIQHLPPQKKEICHLKIDENKTNQEIAEEMGIS
                     VNTVKSHYQESVKMLRDYFQKIKLLLF"
     gene            complement(8856..9647)
                     /locus_tag="PKH68_03110"
     CDS             complement(8856..9647)
                     /locus_tag="PKH68_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11958.1"
                     /translation="MHDPIFSKENYLLKAGHYPEINHREKAILAKLELIDKSDTRLVG
                     LYYLVLWDLFLIGEREDSTYIERLTLARLLRYLYTYESGIILMGKKEPFAVNEISSKQ
                     IREFQDVVKKQLSETFLMVRLHEDAEFHFARKTWSCENASQWPPDKRTIEVLETTTFA
                     MKYMNQDKPVRIVIDKDTIDLIISMEEERVIDEKRTYLGRFLSFINKYATKYGYFHHQ
                     ENTISIPDALFLYDTLVLLEFVQPNQLTDEHDKYLFIKKELKNGK"
     gene            complement(9818..12004)
                     /gene="recQ"
                     /locus_tag="PKH68_03115"
     CDS             complement(9818..12004)
                     /gene="recQ"
                     /locus_tag="PKH68_03115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNX11959.1"
                     /translation="MIKHSELTSHLKKNFGFDTFKGNQEAIINNVLEGKDTFVLMPTG
                     GGKSLCYQLPSLILEGTAIVISPLIALMKNQVDAMRNFSEEDGIAHFLNSSLTRVAID
                     AVKEDVISGKTKLLYVAPESLTKEENIEFLRQVKISFYAVDEAHCISEWGHDFRPEYR
                     RIRPIINEIGIAPLITLTATATPKVQHDIQKNLNMLNATVFKSSFNRANLYYEVRPKT
                     KDVEKDIIKYIRSQAGKSGIIYCLSRKKVEELTETLQVNGISALPYHAGMDSQVRSEN
                     QDKFLMEKADVIVATIAFGMGIDKPDVRYVIHYDIPKSLEGYYQETGRGGRDGGEGQC
                     ITFYAYKDLEKLRKFMQGKPVAEQEIGNQLLLETQAYAESSVCRRKLLLHYFGEEYTP
                     ENCGSCDNCMKPRKFVEGKELVLLVLETVQQLKEKFKAEHIVDVLKGNESPDVKSYQH
                     AELENFGSASDEDEKLLHAVIRQAMIAGYLTKDIETYGVLKMTAAGKEYLKNPTSFPI
                     VKDTEFEDDEDEINGRNAGGTCAADDVLFSILKDLRKKLSKKLEVPPFVIFQDPSLEA
                     MATSYPISMDELQNIPGVGAGKAKRYGEEFLKVIREHVKENEIIRPEDLRVRTVANKS
                     KLKVSIIQSIDRKIALDDIALSKGLDINELLDEIEAIVYSGTKINIDYFLEEIMDPDR
                     VDEIFEYFNTAETDKISTALDELGENDYSEAEVRLVRIKFLSELGN"
     gene            complement(12245..13477)
                     /gene="clpX"
                     /locus_tag="PKH68_03120"
     CDS             complement(12245..13477)
                     /gene="clpX"
                     /locus_tag="PKH68_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNX11960.1"
                     /translation="MAKTVKHCSFCGRTESQVGFLITGLEGAQICNECSEQASAIVRD
                     AKMGGDKASKLDKEKLPKPREIKNYLDQYVIGQNDAKKYLSVAVYNHYKRLMQEKSND
                     DVEIEKSNIIMVGYTGTGKTLLAKTIAKKLHVPFTIVDATVLTEAGYVGEDVESILTR
                     LLQVADYNVKEAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGAIVNV
                     PPQGGRKHPEQKFIPVNTQNILFICGGAFDGIERKIANRLNTRVVGYGASVENEQVDR
                     TNLLQYIAPQDLKSFGLIPEIIGRLPILTYLEPLDRDALRNILTEPKNSIIKQYTKLF
                     KMDDVELTFEPEVLDYIVDKAIEFKLGARGLRSITESIMMDEMFEIPSKKVKKWVVTL
                     EYAHSKMDKMNSHRLKTA"
     gene            complement(13482..14159)
                     /gene="clpP"
                     /locus_tag="PKH68_03125"
     CDS             complement(13482..14159)
                     /gene="clpP"
                     /locus_tag="PKH68_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNX11961.1"
                     /translation="MNNNDFRKYATKHLGMNGLSLDEYFKVSNNGYISPSILEERQLN
                     VTQMDVFSRLMMDRIIFLGTQVDDYTANVIQAQLLYLDSADPGKDISIYLNTPGGSVY
                     AGLGIYDTMQFISSDVATICTGMAASMGAVLLVAGTQGKRSALKHSRIMIHQPSGGAQ
                     GVAADMEINLREMLKLKKELYQIIADHSGKTYEQIEKDSDRDFWMTSQEALEYGMIDR
                     VLVKEKK"
     gene            complement(14368..15729)
                     /gene="tig"
                     /locus_tag="PKH68_03130"
     CDS             complement(14368..15729)
                     /gene="tig"
                     /locus_tag="PKH68_03130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNX11962.1"
                     /translation="MNIVRKDLDQNKATLTVSVTKADYDEKVEKSLRDYRKKANIPGF
                     RPGNAPLGMLRKIYGKALVAEEVNRLVSDGLYNYIKDNNIHILGEPLPAETQGEVDFE
                     NSEEYEIDFEIAVAPEFEVSVSKSDKITYYKIKATDEMINNQIKSYTGRFGKYNQEEI
                     VEENDMVKGELLEMVNGKVNENGIKVKDAVLTPEYMKDAAQKALFVGKTKGDKVVFNP
                     QTAFENEVEISSMLKISKEEAKKLTSDFMMQIEGITRYQEGEVNQELFDKVFGEGAVT
                     SEKEFRNKIAESIQENLTADSDYKFGIDAREVFTEKYNHLQFPTNILKRWLKVANENL
                     TDEKLEEDFPRIINDLIWQLVKEKIVKMNDIKVETSDVQEYARKVAKSQFAQYGMVGL
                     EDSIIDNYVKDMLKKEETVKNFIDKALEEKVIAYIKTAVKLNEKEISVEDFNKLFETE
                     AAN"
     gene            16102..17289
                     /locus_tag="PKH68_03135"
     CDS             16102..17289
                     /locus_tag="PKH68_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX11963.1"
                     /translation="MKKTILFVSLILGLSTLTAFSQGIQFEENNDLKAALVKAKAENK
                     LVFIDAYAVWCGPCKVMARDIFPLKEVGDFFNAHFVNLKLDMEANGNLEIAKKYEVKA
                     YPTYLFLNAEGELVHQGLGSMPADKFIEVAKTATDSENNFSALSGKIKKGDLSLTTIN
                     KYLAQNPYDKGNGALVESYFQTLSENQIYTKENWELFNNFVDDISSPSFQYFIQNREK
                     IAGFIGKKQVDEKIYKTMGNAYFQNPGTEASLKSIDPELFGKVKTRVDMMKANGKFNQ
                     NKNDKAAWDNLIKVSSLAQNQITNPTELNNMAWLVYENYKKFNDKNALKSALGWAKKA
                     HETAPDQDFIADTYAHLLFETGKKKDALKMETKALDLATKAGNTERVEEFKKAIQKFK
                     GRK"
     gene            17359..>18364
                     /locus_tag="PKH68_03140"
     CDS             17359..>18364
                     /locus_tag="PKH68_03140"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNX11964.1"
                     /translation="MAPKPFIYQDPFPIGKEKTEYYLLTKDFVSVEKCGDREILKVEP
                     EGITALARTAMRDCNFLLRPEHQKQVAHILADPEASENDKFVAITMLRNAEIAAKGVL
                     PFCQDTGTATVYAKKGNNVWTDGTDEEALSKGIYETYTQENLRYSQNAALNMYDEVNT
                     GTNLPAQIDLVASHGDEYKFLFIAKGGGSANKSYLFQETKALLNPVSLEKFLVEKMKT
                     LGTAACPPYHIAFVIGGTSADACMKTVKLASTKYYDELPTTGNELGQAFRDVEMEEKV
                     LKAARESGYGAQFGGKYFAHDIRIVRLSRHGASCFVGMAVSCSADRNIKGKINKDGIW
                     V"
BASE COUNT         4932 a         4003 c         3687 g         5642 t
ORIGIN      
        1 tcacggcaca ggtgtcagtt ccgcgataag ccgaaatgta gttttgaatt tcgttgtttc
       61 cccatcctcc ggcaccggtt tcgtaccacc agcgggttgt actgggcaac ggagtttttc
      121 cattggtctg acgggcatca ttgaattcat cctgccattt gagcgtatat ccggctggca
      181 cgtaattttg aatggttccg gctgcttgtt taacggtgaa ctgtttggtg taactgcccg
      241 atttaaatgt aagtacagcc gtgcgttcag cttctgcact attattcatc gcggtaattt
      301 tgagactggt ttgtccgtta tatcccactg tgggagaggt ggtacaccac gtctggctgg
      361 atgttaccga ccaggttccg ttggaggtaa ctgttaagtt ttgggtactg gccccggacg
      421 ttaatgtaat actttcggga taaaattgcc aggtaatatc aactgttgga ggctcattgc
      481 cggttccggg tttattcccg cagcccgaca gtaaaaccaa agctataagt aataaatggt
      541 gcattgtttt catggtttgt ttttgataca ttgcttagaa cgctgaagtt aatcaatgta
      601 acaacgtgtt ccatcacagg tttattttgt taacaaaaaa aaggaagggc tactgcccgg
      661 cagccgcttc cgtttttttg aaaggagtgc taattattat tctgtaaaag aaaaatcatc
      721 aaagaagaat aatcccggtc cggcatgacc ttcggcacca aactgaatca cgattttgtc
      781 gtaatcagtc cgggaagcca cattgctgaa atcgaaagtc agttcaatcc aggtattcaa
      841 cgccagatcg gctttcacta tttcagtctg cgactgccag gcattaccgc ccaaactgct
      901 gtcctggagt tttactgcca gttgtggacg cagttttgca ttcgaaatcc acgaacctgc
      961 cgtagcccaa tcagtggtat aatcgttgga tgggatatac acttttactt taatcttgtt
     1021 ttgtttggtc agatcgaatt tatatttttc ggctacaaaa ttaagattgc tgtagaaagc
     1081 agtagatttc tgataccggt acactttgtt cgactcgttg ataggcaccg gagccggatt
     1141 atcaaagatg ccgcttttac ttcccatatc ttcagctaca aacttgatgc ccgaagttcc
     1201 ggtttcaaaa ttttcagcca gtggcaccgc tttcggttct ttcactaccg gaggagccgg
     1261 cacattcaat tcctgcagac aatatatgaa tacccaatcc tgaccttcta ctgtgttatc
     1321 cacacgcaat gccatcactt tatcggtttg ataaatgatt tcgtacactt gtgaaccaca
     1381 ataatagccc ataaaggcat tgccggtaag tgttattttt ggatattttc cactttcatc
     1441 taaagcaaaa ttgtaattac ctccattata agcgaataaa gcatcatcac catcaaccgt
     1501 tgttacgtta aaacctccct tagtagctgc tgatgctttg cgcccatagc cttcaccttt
     1561 gtttacaata ttgagtttaa cgcctgcctg aataaaggta aacttgaaat catacaattt
     1621 ccatgtttct ttggcgttga cacctgctcc ccaccatgat tgcccgaatg aaccctgagg
     1681 tccaaggccc atatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccca
     1801 gcgtacaata tcccagaaac ggataccttc acccacaaat tcacggcgac gttccagctt
     1861 gatgttggcc acggtagcag gtatcggttt gtccattcca aatgcacgtt tgcgaacgcg
     1921 gtccagacag gattgagctg taatgccatc cacaacggac tgtccgtgaa ctaccaccat
     1981 ttcggcataa ttcagtaatg tttctgaaaa acggaaaatt ctgaaattat tgcaatagtt
     2041 caaatccaca tctccctgag gattgtaacc aacgcgagca gaatattttg ccataaacag
     2101 gtgtgtatcc tggaaacggg cagtatactg tgatgcttcc catttattga tggagccttc
     2161 gcgacgggta tcaccctctt catacatatc ccaggtagcc tgacgtaccg gaccaaatcc
     2221 ccatccgcct ttgaaatcac cggttttact tccggtattc aatccgttgg gcgaaatgaa
     2281 tgcgggtaaa ttggtaccat aaccctgcca accgctcgac caggtttttc cttcgggcaa
     2341 ctgattgctt tcgaaaatgg attccttgca gaattcgttt tcgtcgagcc acatagctcc
     2401 aaagttggac atcagatcaa aatcattact tttgattatt tcagccatat ctttggttac
     2461 ttcaccgtat cgggcggcat ccttctgata cattaccacc cgggctttca gcatcagggc
     2521 tgcagctttg gttacccggc ctgaatttgg agtaccctga gcatagttca tcggaagtcg
     2581 gtcagtgccg ccatctgatt taccacaggc aaaatcaaga tctttcatga tagcagcata
     2641 cacttcattg gctgtattct gcttggtcat gtaaggcggt gtggtgagcg gcgcttcaaa
     2701 atagggaatg tttccccaga atttccaaag ccagtgtaca taatatgcac ggagaaaatg
     2761 agcttcagct ttgtattgat ttaattttgc atcgggaaca ttcacagcat tgtcacaagc
     2821 aattattacg ttgttacaac gagccagtcc ggtataaaat atactccaaa gtccgtctat
     2881 attttcattg ggagtagttg taaacaacga aagcaggtac aactgatgct ggtcaccggc
     2941 atcaccgcca cccttgtaaa tatcatccga ccgtaaatcg gacatgagcg gaatactgtt
     3001 gtagttatta ttagcataac tatcaaataa cagaatctga taggcagagc ccaaattgga
     3061 caatatggct ccttcagtgg cagaacctcc tgccgcttgt ttttcggtgg ggagagctgt
     3121 caaaaagtct tcaccgcaag atgtaaaccc gataagggct acaaatacta taaatatata
     3181 ctttttcatg tgtatcatat tgttttagaa ttaaagactt atgttagcac cgactgaaat
     3241 cgttcttgac tgcggataga tgccccggtc aataccgata gtggtatatc ctccggatgc
     3301 cacttcagga tcaaaaccat cgtatccggt gaaagtcaac agattttctg cctgaataaa
     3361 caatctcaat ttttcgatgg atgctttacg ggtcaacgat ccgggcagtg aatagcccag
     3421 ctgcgtagat ttcatacgaa cgtaggaacc gtccttgata tacaagtctg acgaacgcca
     3481 gttgctgtta gcattggcta tggtcattct gggtattgta aaggaggtac cctcaccatg
     3541 ccagcgttgc agtatccatt ccggacgatt catagcaggt atgtcacctc gttgtgagaa
     3601 atcgaaaata tcgttaccct gtgtaccctg gaagaatacg ttcaaatcga aatttttcca
     3661 ttcggctccc agggtcagac caaaggtcca gtcgggcata cctttaccga tttttacctt
     3721 atcatcgtcg ctgataattc catccatgtt ggtatctaca aacctaacat cacctggttt
     3781 agccaccgac tggtattttt cgcccttgtc attcacatat gcatcgacct ctgcctgatt
     3841 ttggaacaga ccatttgttt tgtatccgta aaaatacggg aatacttcac cgttctttcc
     3901 tttcacgtag ctacccactc ctgaagcgcc tgcattttcg taaatctgtt caccggtggc
     3961 attaccaagt tttatcaatt tgtttttcag atatgaagca tttgcagaca agaagtattt
     4021 gaagtctccg atggattgtt tccatccggt ttcaaactca agtccccagt tttgcatatc
     4081 gcccacgtta gcgattggag aaccctgacc aacatacgtt gggataggtt gttccatcaa
     4141 catgccgttg gtggtttttt tgaaataatc aaaactgaat gtaaaggctc ttttgaagag
     4201 atacgcttca aagccgacat cggtttgttg cgattcttcc catttcacat ccggattggg
     4261 gatggaagcc ggagaacttc cgtactgcat gattccgccg gtttcgccca ctttggtcgg
     4321 gtcgccgggg tttaccttat atccgccgcc aaagtagtag ttttggccac catccataag
     4381 cgatgtataa cggaatgctc caatacgttc gttaccgttt tttccccagc tgaagcgaag
     4441 tttcaatgca tccatccatt tgggtctgtc ctgcataaaa gcctcattgg ttaagttcca
     4501 tcccagcgaa aatgccggga aagtagccca tttatgtgta gggccaaaat tggacgaacc
     4561 atcccgacgt atcgttgcct gaagaatata tcgttcgtca aagttgtaat ccaagcgggc
     4621 aaaataggat gccagtgacg ccagattgta tccaccggtt ccaccccaaa cccgttcgag
     4681 ttttcggtcg gcaatggcag agttgatatt tgctttcagg ggatcggttt cgagcagttg
     4741 ctggtcattt ccacccagat tgcggtaggt atatttttgg gctgattgac cgagaatgac
     4801 ttccacatta tgtacattag cgaatgtttg attgtaggaa agtgtatttt ccacctgcca
     4861 tttgaaaccc cggttcattt cactctgcac cgtgcttaca tccacggttt taccctgagt
     4921 ggccagaaaa tacgggaatg tataactgtc gcttccccag aaagccaggt caacaccgta
     4981 agtgctctta aatttcaatt gtggaagaag acttaactct ccccagaaag ttgaaacgat
     5041 tttatcgctg ttgcctattc cgctgcttgg ctggttgagc ataccgaccg gattggctat
     5101 ttcctgaaat cccgatggag gaatggaaaa tacacgtccg tctttatctt tcacggcatt
     5161 aggatattga gccaggatag cggcagcagt agcttcgtcg gcatatacag gaaccagcgg
     5221 agagaaggtc agagcgctac caagaatgga accatattcc gagttggcat caatagtgga
     5281 acttttatct cttgaatagc ccaaattcac gccaattctc agttcgttta agaagtctct
     5341 gcttttttct tcgaatatgg tatgggtgga attggaacga acgctccagc ggttgtagtt
     5401 cgatttaccg taatctccac caacaatacc ttgttggtcg aaatagccta aggaaaggaa
     5461 gtattgtgat ttttcggttc ctccggaaac gctcaactga tggcttttta ccggtgcatc
     5521 ataattgaaa gttgcatcct gccagtcggt tccttctatt ccgctcatta cctgttcgtt
     5581 ggaataccgg ggtaaaccac catcgttaat caggccttca ttcatgatta ccatgtattc
     5641 ctgtgcattc agcactgatt ttttcttcca tgggttttgc catccgtaac ttacgttgta
     5701 gttaatctgt gttttgccga ttgctccgtt tttggttgtt accagcacca caccattagc
     5761 agcacgtgct ccgtaaatgg cagccgatga agcatccttc aatatttcta ccgattgaat
     5821 gtcaacgggg ttcaggtagt tgataccacc atccacgggc attccatcga caatataaag
     5881 aggttcactg ttatttacag taccaatacc gcggatacgg actttggaat ctgagccggg
     5941 ttgtccggaa ctttgagtaa tctgaacacc tgaaactttg cctttcaata catcttctac
     6001 gcgggaaggt tttgtgttat tcagttgatc ggcagttacc cggctgatag cagcggtaac
     6061 cacactcttc ttctgcacac cgtacccgat taccaccagt tcatccaggc ttgctatatc
     6121 ttcacgaagc tgaacattta aggtcgattg gctggtaacg gtaaattcct gggaggtgta
     6181 accgatgtat gacacccgca atactgcacc gcgaggaacc gacagggtga aatcaccgtt
     6241 aacattggta atggtgccta atccgctgtt tggaatagcc acacttgcgc ccatcaccgg
     6301 atttccattt tcatcggtaa ctttacctct gatctgtaca ttatccgctt gctgagccgc
     6361 tttttgtccg gattcaatgc ccgaagaatt gtcggcaatg aaaatcttcc gatccctgat
     6421 atcgtaactc aggttacggg gagtcaataa tttagccatt acatcctgaa tggaagcatt
     6481 gcgcacattg atgctgacaa tttcattcag attaacgacc tctttgctgt aagccaacgt
     6541 atagccggtc tgacggctga tactttctaa gacctgtttt aaactttgct gacttgaatt
     6601 taaacttacc ttctgtgcat agcccatact gctcaggcct ataaacatta gtgtaaataa
     6661 aatcaacgtc cgccttttaa aaagttgttt tctcatggta taactttttt tagtgaaaca
     6721 ttaattatta gaaataattt attcagatat tttaaagaac gtgttttcga aataagaatc
     6781 tcaggggtta tagggtttta atttcctgaa ttccggacaa ttaaaagaat aatatcaaaa
     6841 aaaaaatcag attaaggtgt actaaagtga ataaaaagaa acaaaatgga tgtcaacatc
     6901 gaatcattga cacatgggga taataaaagt atgaggtaaa aagaaaatta ttttaaagaa
     6961 actatgatac tgttatcttt tcgaatggaa aattttaccg gtgcaatttt ttccagctcc
     7021 ttcacaatgt tttcgagcga tgtctgattg gtggtgagtg tatatgtata ggtaagtgct
     7081 tttggattta gtatggcaaa ttccacatta tatcttcggt tcagggtttt tatcacgtcg
     7141 gccagtggtg tatcccgaaa taacactata ttatctttcc atacagaaac ttcgtagggt
     7201 ttgtccacat aatcgatgac acatttgccg gtaagtacat catatactgc ttgttgaccg
     7261 ggcagtatct gataactgtt ttgaggtgta ttaaatacta tactgccttt atcaaggagt
     7321 attttcattt cgttgtcttc cttaaaggca tgaacgttga aggaagttcc cagcactgtg
     7381 acatcggtat tgtttatgcg aacgataaat ggtcttcgct tgttggtagc aatattgaaa
     7441 taaccctcgc cctgtagaaa gatttcgcgc tttgacaatc cgaatttacg gggatatcgc
     7501 aaacgggtat ctgagttgag gtatgcctca ctcccatcct gaaaaataat gtgttcctgt
     7561 ttgcctttgg gaacatacac ttcagaataa accgttttgc cgaataggtt tacctgcgaa
     7621 ttcaaccgaa tggtgagccc caccaccagt attaagggaa taagtgcagc agctacctgc
     7681 cagtaaatac gacgtgtttt tttgatccgt attcgtttat taatcactgc aagcatattg
     7741 gcggactgca ctgccgataa gtcactgtat cgatcggggt tcgattctat gaaagcagct
     7801 tcgtgatcca tgagttctga aagatatgcc tgaccttcgg cggtgtccga aaaccattca
     7861 gccacctgac gtgcttcttt tttggtggct gtaccattca gtactttatc tatcgtttct
     7921 ttatcgatcg ggtgcatagg gttttatatc tttatctact ttattgacac agaagatgaa
     7981 aaaaatatga gttacaattg ttaataattc aaaaaagcag cagttttatt ttttggaagt
     8041 aatcccgaag cattttcacg gactcctgat agtgcgattt tacagtattt acagatattc
     8101 ccatctcttc agctatctcc tgattggttt tattctcatc aatctttaag tggcatattt
     8161 cctttttctg aggtggcaag tgctgaatgc cttcgtagag cagactgcgg agttgcttat
     8221 cctccattat gctgactata tcactgcttt cgtccgttat ttctatttga gcattctcat
     8281 aattcaacga tatcatttcc ttattatccc ttatttggtt gagcacaaaa ttcttagtca
     8341 tggtataaag ataatttttc atatttatat ccacatcaat ataccttgcc gaatcccaga
     8401 gcttgacaaa aacatgctgc acggcatctt cggctaattc aacatctttc aggtatctaa
     8461 aggccaaact gtacaggtag tggtgatact tgtgatatac aaccgtaaaa gcttctttat
     8521 ccctcttttt aatcaggagg aataatgaac tgtcttcgtt attggaattc attgagaatt
     8581 tgactcgcat agctaagcag taaaattgct ttttattata aggcaaatgt aacgattatt
     8641 tatttctgtt tattctacaa atatgcgatt ttcaaattat tttatggcat gcacatcctt
     8701 caagatcacc ggcaacatag atatactatc aaaatgatat ttttcagctg acattaagtt
     8761 tcttatctta aaaaaacaaa gccttacttt catattgtaa gactttattc ttcaaatgga
     8821 cttttcaata ataccggcat aatcttttcc ccggcttatt ttccgttttt caattctttt
     8881 ttgataaaga ggtatttgtc atgttcatcg gtgagctgat ttggctggac aaattcgagc
     8941 aataccaacg tatcgtacag gaaaagtgca tcgggaatgc ttatggtatt ttcctggtgg
     9001 tgaaagtagc cgtatttagt tgcatactta ttgataaaag agagaaatcg tcccaaatag
     9061 gttctttttt catctattac ccgttcctct tccatggaaa ttatcaggtc aatggtgtcc
     9121 ttgtctatta ctattcttac cggtttgtcc tggttcatgt acttcatggc gaaagtggtg
     9181 gtttcgagca cttctattgt tcgcttatcg ggcggccatt gcgaagcatt ttcacacgac
     9241 caggtttttc tggcaaaatg aaattctgca tcttcatgga gcctcaccat aaggaaagtt
     9301 tccgacaatt gttttttgac tacatcctga aattcgcgta tttgtttgct cgaaatttca
     9361 ttcactgcga atggctcctt ctttcccatc aatattattc cactttcgta ggtataaaga
     9421 taacggagta aacgggccaa tgtcagcctt tcgatgtaag tggagtcttc tctttcaccg
     9481 ataagaaaca aatcccaaag tacgaggtaa taaagtccca ccagcctcgt gtcactcttg
     9541 tctatcaatt ctaatttagc gagaatggcc ttttcccggt gattaatctc gggataatgt
     9601 ccggctttca aaagatagtt ctctttactg aaaataggat catgcatggt ttaggtgact
     9661 taaaatgtaa ataatttcag acaaatggtt tatttgtatt tataacaaat catttccgtc
     9721 ttaagttgct tttacaattg ctaaaagaac aaaaaaaatg caggaagagt ggtatatctt
     9781 cctgcaaaaa taaaacttat tgaaaaaaac tacattttta gtttcccaat tcagataaaa
     9841 atttgatccg gaccagtctc acttcggctt cactgtaatc attttcgccc agttcgtcga
     9901 gtgcagtact gattttgtcg gtttcggcgg tgttgaaata ttcaaatatc tcatctacgc
     9961 gatcgggatc cattatttcc tccaggaaat aatcgatatt aatttttgtg ccggaataga
    10021 cgatggcttc aatctcatca agcaattcat tgatatcgag cccttttgaa agtgctatat
    10081 catccagggc tatttttcgg tcgatagact ggatgatgga taccttgagt tttgatttgt
    10141 tggctacagt ccgtacccga agatcctcgg gacgaatgat ttcgttttcc ttaacatgtt
    10201 cccgtatcac tttgagaaat tcctctccat agcgttttgc tttaccggca cccactccgg
    10261 gtatattctg gagttcatcc atggatatag ggtagctggt tgccatagct tcgagcgatg
    10321 ggtcctgaaa tataacaaat ggcggaactt cgagtttttt cgagagtttt ttacggagat
    10381 ccttcaggat ggagaagagc acatcgtcgg cagcacaagt accaccagca tttcgcccgt
    10441 ttatttcatc ttcgtcatcc tcaaattcgg tgtcttttac gatggggaac gaagtcggat
    10501 ttttcagata ttccttaccg gcagctgtca ttttcaacac accataggtt tctatatctt
    10561 tggttaagta tccggctatc attgcctgac ggattacggc atgcagcaat ttttcatcct
    10621 catcggaagc cgatccgaaa ttctcgagtt cggcgtgctg atacgatttc acatcaggcg
    10681 attcattccc tttcaataca tccacaatat gttcggcttt gaatttttct ttcagttgct
    10741 gaacagtttc caatactaat aatactaatt cttttccttc cacgaatttt ctgggtttca
    10801 tacagttatc acaacttccg caattttcgg gtgtatattc ttccccgaaa taatgcagca
    10861 ataattttct tctacataca gatgattcgg cataagcctg agtttcaagc aggagctgat
    10921 tgccgatttc ctgttcggct accggttttc cctgcataaa tttacgcagt ttttccaggt
    10981 ctttataggc ataaaatgtt atgcactgac cttcaccacc atcgcgtcca ccccgtccgg
    11041 tttcctgata atatccttcc aggcttttgg ggatgtcgta gtggatcaca tagcgcacat
    11101 cgggtttgtc aattcccatt ccgaaggcga tagtagccac gatgacgtcg gctttttcca
    11161 tcaggaattt atcctgattc tcgcttcgaa cctgcgaatc cattcctgcg tgatagggca
    11221 atgcagatat tccgttcacc tgaagggttt cggttagttc ttccaccttt ttgcggctca
    11281 ggcagtaaat aatgcccgat tttccggctt gtgaacggat atattttata atatctttct
    11341 ctacgtcctt agttttaggc cgaacctcat aatacaggtt cgcacggttg aacgaagatt
    11401 tgaacactgt agcgttcagc atattcaggt ttttctgaat atcgtgctgc accttgggag
    11461 tggctgtagc ggtcagtgtt atcagcggag caatacctat ttcgttgatt ataggtcgta
    11521 tacgccggta ttccggcctg aaatcatgtc cccattccga tatgcaatgt gcttcgtcca
    11581 ccgcataaaa ggagattttt acctgtcgga gaaattcgat attttcttcc ttggtcaatg
    11641 attcgggtgc cacgtagagt aatttcgttt ttccggatat aacatcctct tttacagcat
    11701 caattgccac ccgtgtgagc gatgaattga ggaaatgggc aatgccatct tcttcactga
    11761 aattacgcat ggcatccacc tgatttttca tcagtgcaat gagcggagat atcacaatgg
    11821 ctgtgccttc cagtatgagc gatggaagct ggtaacacag tgacttgcca cctccggtgg
    11881 gcatcagtac aaaggtatcc ttaccttcaa gcacattatt gataatggct tcctgattgc
    11941 ctttgaacgt gtcaaagcca aagtttttct tcaaatgtga tgttaactcc gaatgcttga
    12001 tcatagtaat tgctcttcaa agattgaaaa cgaaattaca tttaattttt atattacaca
    12061 aatatatcac ttatcagatg aaatatttta aattttatct gaaattttat tttagcaaaa
    12121 gtaaaaacag atttgaaata aacaaggtct ttgaaaaaaa aaacaaggat attagcattt
    12181 ttttatacta acatcctcat ttgctttatt ttattaatac ataatctacg ataatttaga
    12241 aagattatgc tgttttaagg cggtgagagt tcattttatc cattttggaa tgggcatact
    12301 ccagggttac cacccatttc ttcacttttt tggaaggtat ttcaaacatt tcgtccatca
    12361 tgatggactc ggtgattgag cgcagtccgc gagcaccaag tttgaattca atggctttat
    12421 ccacaatata atcaagcact tcgggttcga aggtgagttc cacatcgtcc atcttaaaca
    12481 attttgtata ttgtttaata atggagtttt tgggctctgt gagtatattg cgcaatgcat
    12541 ctctgtccag tggctccagg taggtgagta tgggcagacg tccgatgatt tcggggatca
    12601 gcccgaaaga tttcaaatcc tgaggtgcaa tatactgcag caggttggta cggtcaacct
    12661 gttcgttttc caccgatgct ccatatccta ccacccgggt attgaggcgg ttggctattt
    12721 tgcgttcgat gccgtcaaaa gcacctccac aaataaagag gatattctgg gtattgaccg
    12781 gaataaattt ctgttcggga tgtttgcgtc cgccctgcgg tggcacattg acaatggctc
    12841 cttcgagcag tttcaacaga ccctgctgta ccccttcgcc gctcacatcg cgcgtaatac
    12901 ttggattgtc gcttttgcgg gctattttat caatctcgtc gatgaataca atgccccttt
    12961 cggcttcttt cacattataa tcggccacct gaagcaaacg ggtcagaata ctttccacat
    13021 cttcgcccac atatcctgct tcggtgagta ccgtggcatc gacgatggta aagggaacgt
    13081 gcagtttttt ggcaatagtt ttggcgagca gcgttttgcc tgtgccggta tatcccacca
    13141 tgatgatatt ggatttttca atttccacat catcgttgga tttttcctgc atgaggcgtt
    13201 tgtaatggtt gtaaaccgct accgaaaggt atttttttgc atcgttctgt ccaatgacat
    13261 actgatccag gtaatttttt atctcccgtg gtttcggtaa tttttcttta tcgagttttg
    13321 atgccttgtc gccacccatt tttgcatcgc gaacaattgc agaagcctgt tccgaacatt
    13381 cgttacaaat ctgagcacct tccaggccgg taattaaaaa acccacctgg gactctgttc
    13441 ttccacagaa gctgcaatgt ttgactgttt ttgccatata attatttttt ctcctttaca
    13501 agcacgcggt ctatcatgcc atactccaac gcttcctgcg aagtcatcca gaaatcccgg
    13561 tcactgtcct tttctatttg ttcgtatgtt ttgcctgaat gatcggcaat aatctgatac
    13621 agttcctttt tgagtttcag catttcgcgc aggttgattt ccatatcggc agccactccc
    13681 tgtgcgcctc cgctgggttg atggatcatg atgcgcgaat gtttgagtgc cgatcgtttg
    13741 ccctgtgtgc ctgctactaa aagtacggcg cccatcgagg cggccatacc ggtacaaatg
    13801 gtggccacat cggacgaaat aaactgcatg gtatcgtaaa tgcccagtcc ggcatatacc
    13861 gaacctcccg gagtattgag ataaatggag atatctttgc ccggatcggc cgagtccaga
    13921 tacagcaact gtgcctgaat gacgttggca gtataatcat cgacctgagt gcccagaaaa
    13981 ataatccggt ccatcatcag gcgggagaat acatccattt gagttacatt cagctgacgt
    14041 tcctccagaa tggaaggtga aatatagccg ttgtttgaca ccttgaaata ttcatcgagc
    14101 gacaaaccat tcatccccag atgtttggtg gcgtatttgc gaaaatcgtt gttattcatt
    14161 ttttttgttt taataatttc taaatttaag acattggaat acactgcgaa tatcctgcca
    14221 gacttttata tctgccattt tgtcagttat tgaaatttat gtcagtattt aaaaacagag
    14281 aaggctttta aatgtaatct cagcaaaggt aaactatttt gttgatcaaa cagatttaaa
    14341 aaccttttcg gttatcattc agaaatatta attggcagct tcggtttcaa acaacttgtt
    14401 gaaatcttct actgagattt ctttttcgtt tagtttcact gctgttttta tataagctat
    14461 cactttttct tccagggctt tatcgatgaa gtttttcacg gtttcttcct tcttgagcat
    14521 gtccttcaca taattatcta tgatcgagtc ttcaagtcca accatgccat actgagcaaa
    14581 ttgcgatttt gccactttgc gggcgtattc ctgtacatca ctggtttcaa ccttgatatc
    14641 attcatttta acgatttttt ccttcaccag ttgccagatc aaatcattaa tgatgcgagg
    14701 aaagtcttct tcgagttttt cgtcggtcag attttcgtta gcaactttca gccagcgctt
    14761 gagtatatta gtcgggaact ggaggtggtt gtatttttcg gtgaaaactt cacgggcatc
    14821 gataccaaat ttgtaatcgc tgtcggcagt gaggttctcc tgaatacttt cggctatttt
    14881 gttgcggaat tctttttcac tggttacagc accttcgccg aatactttgt cgaacaattc
    14941 ctgattgact tcgccttcct ggtaacgggt gatgccttca atctgcatca tgaaatcgga
    15001 agttaatttt ttagcttctt cctttgaaat tttcagcatg gaactgattt ccacttcgtt
    15061 ttcgaaagct gtttgtgggt tgaataccac tttatctcct ttggtttttc ctacgaataa
    15121 tgctttctga gctgcatctt tcatgtattc aggtgtcaga actgcatctt tgactttgat
    15181 gccattttcg ttaacttttc cattcaccat ttccaggagt tcacctttca ccatgtcgtt
    15241 ttcttccaca atttcttcct gattgtattt tccgaaacgt ccggtatatg atttgatctg
    15301 attgttgatc atttcatcgg ttgccttgat tttgtagtag gtaattttgt cgcttttcga
    15361 cacgctcacc tcaaattcgg gagccacagc aatttcgaaa tcgatttcat actcttcgct
    15421 gttttcgaaa tcaacttctc cctgtgtttc agccggaagg ggttcgccca aaatatgaat
    15481 attgttgtcc ttaatataat tgtacaaccc gtccgaaacc aatctgttga cttcttcggc
    15541 tacaagggct ttgccataaa tttttctcaa catgcccaat ggcgcattgc ccggacgaaa
    15601 gcccggaata ttggcttttt tgcggtaatc acgcagactt ttctctactt tttcgtcgta
    15661 atcggctttc gtaacactta cggttaaggt tgctttgttt tgatccagat cttttctgac
    15721 aatattcata tcctgtttat tattagcaca ttgtgcattt gtaaattttc gaatttaata
    15781 ggggtgcaaa ggtacaaaaa cttgagataa ttcagacatt atttaggtaa aaaagataaa
    15841 aatcatcaaa ttttgccctt tatctttccg catgatccat acaagattga ttatataaac
    15901 aaataatgac agttcgggtt aattaattct aattaagcaa ttaactttta gttcttcaaa
    15961 aggctccatt caaaatatca cttttttttg taattttgtc gggcatccgg tgtacgggta
    16021 tcaatgtggc atttctttcc aaactaaaaa cgcaagtcat taacaataaa gatttaacaa
    16081 tcaaaataaa acacaatcaa catgaaaaaa acaatcttat ttgtaagtct gattttgggc
    16141 ctttcaacat taacggcttt ttcacaggga atacagtttg aggaaaataa cgacctgaag
    16201 gctgcgcttg taaaagcaaa agctgaaaac aaactcgttt ttattgatgc gtacgccgta
    16261 tggtgcggcc catgcaaagt gatggcgcgt gatatattcc cgctcaaaga agtcggagat
    16321 ttttttaatg cccattttgt gaacctgaaa ttggatatgg aagccaacgg aaatctggaa
    16381 attgccaaaa aatacgaagt aaaagcctat ccgacctacc tttttctgaa tgccgaaggc
    16441 gaactggtac atcagggact gggcagcatg ccagccgata aatttataga agtggctaaa
    16501 acggcaactg acagcgaaaa caacttcagc gctttgtcag gaaaaatcaa aaaaggtgac
    16561 ctcagcctga ctaccatcaa caagtatctt gctcaaaatc cttacgacaa aggcaacgga
    16621 gcgttggtcg aaagctattt ccagaccctt tccgaaaatc aaatctacac aaaagaaaac
    16681 tgggaactgt ttaataattt tgttgatgat atttcttctc cgtcgtttca atatttcatc
    16741 caaaacaggg aaaaaattgc cggatttatt ggtaaaaaac aggtagatga aaaaatatat
    16801 aaaacaatgg gtaatgccta ttttcagaac ccgggcacag aagcaagtct gaaaagcatt
    16861 gatcccgaat tgtttggaaa agtaaagacg agggtggata tgatgaaagc caatggtaaa
    16921 ttcaatcaaa ataaaaatga taaagcagcc tgggataatc tgataaaagt ctcttcactc
    16981 gctcaaaatc agattactaa tcccactgaa ttgaataaca tggcctggct ggtgtatgaa
    17041 aattataaga aattcaatga taaaaacgcc ctgaaaagcg cactcggctg ggcaaaaaaa
    17101 gcccatgaaa ctgctcccga ccaggatttc attgctgata cgtatgcaca cttgctattc
    17161 gaaaccggca aaaagaaaga tgctctgaaa atggaaacca aagcattaga cttagccaca
    17221 aaagccggaa atacagaacg ggtggaagaa ttcaagaaag caattcagaa atttaagggt
    17281 cggaaataat tcttttttcc accatttttt tcctaattca taatttataa ttcataattc
    17341 ataattatat aaaagactat ggcaccaaaa ccatttattt atcaggatcc gtttccgatt
    17401 ggaaaagaaa aaaccgaata ttacctgctg acaaaagact ttgtaagcgt ggagaaatgc
    17461 ggcgaccgcg aaatactgaa agtggagccc gaaggcatca cagcactggc acgcactgcc
    17521 atgcgcgact gcaattttct gcttcgcccc gagcatcaga agcaggtagc ccacatcctg
    17581 gctgaccccg aagccagcga aaacgacaaa tttgtggcca tcaccatgct ccgcaatgcc
    17641 gaaatagctg ccaaaggcgt actgccattt tgtcaggaca ccggcactgc taccgtgtat
    17701 gccaaaaaag gcaacaacgt gtggaccgac ggcaccgacg aagaagccct ctccaaaggc
    17761 atttacgaaa cctacacaca ggaaaacctg cgctactcgc aaaatgcggc actcaatatg
    17821 tacgacgagg tgaacacagg cactaacctg ccggctcaga ttgacctcgt agccagccac
    17881 ggcgacgagt ataagttcct gtttatagcc aaaggcggcg gctcggccaa caaatcgtac
    17941 ctttttcagg aaacaaaagc actgctcaat cccgtttcgc tcgaaaaatt tctggtggaa
    18001 aaaatgaaaa cgctcggtac agcagcctgt cctccttacc acattgcatt cgttatcggc
    18061 ggaacatcgg ccgatgcctg catgaaaacc gtgaaactgg cttcaactaa atactacgac
    18121 gaactaccca ctaccggtaa tgaactcgga caagcattcc gcgatgtgga aatggaagaa
    18181 aaagtactta aagctgcccg cgaatcgggt tacggcgctc agtttggagg aaaatacttt
    18241 gctcacgata ttcgcattgt ccgcctgtcg cgccacggtg cttcctgctt tgtaggaatg
    18301 gctgtttcgt gctcggccga ccgcaacatc aaaggcaaaa tcaacaaaga cggcatttgg
    18361 gtgg
//
LOCUS       DAOPZK010000030        18318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3188_length_18318_cov_21.413842, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000030 DAOPZK010000000
VERSION     DAOPZK010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18318
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_18318_cov_21.413842"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /gene="rpmB"
                     /locus_tag="PKH68_03145"
     CDS             complement(<1..205)
                     /gene="rpmB"
                     /locus_tag="PKH68_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNX11965.1"
                     /translation="MSKICQITGKKAMGGCNVSHSKRHTKRTFDVNLFTKKFYWVEQD
                     MWISLKISAAGLRTINKIGLDAAI"
     gene            complement(393..1625)
                     /locus_tag="PKH68_03150"
     CDS             complement(393..1625)
                     /locus_tag="PKH68_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNX11966.1"
                     /translation="MIEIITIGDEILIGQIVDTNSAWMAVELNKAGFQLAQITSVHDE
                     ADHIKKALDEALLRADVVLMTGGLGPTKDDITKQTLCEYFGTKLVFNPEVLENIQQIY
                     HTRQSVMNELTKSQAMVPEKCTVIQNRAGSAPITWFEKEGKVIVSMPGVPLEMKKVMS
                     EEIIPRLQKHFKTPAIIHKTVQVYGIPESQLALRLTEWENSLPEYLHLAYLPNFGIVK
                     LRISGAGQDKYKLEEAINQQVETLKSILGESIFAYEDKPVEKIIYEKLKSSGLTVSTA
                     ESCTGGNIAHRLTLIPGISDCFKGTVVAYHNELKINMLGVSAQDIEQYGAVSSQVATQ
                     MAEGARKVMQTDLAVATTGIAGPTGGTYEKPVGTIWISVSSPERTITRSFNFGQFARE
                     NFIERSTMAALMMLNEMI"
     gene            complement(1691..2284)
                     /locus_tag="PKH68_03155"
     CDS             complement(1691..2284)
                     /locus_tag="PKH68_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNX11967.1"
                     /translation="MILIDDTILSFDLFDEKFVCDLASCKGICCIEGDSGAPLDWTEI
                     DILEEILPQIWDDLSPASQNLIKKQGVFYLDQEDEPVTSIVNGRECVFTYTDGQGICK
                     CAIEKAFREGKTDFYKPISCHLYPVRLQKNKEFTAVNVHKWIVCECARVQGKNLGVPV
                     YKFLKEPLIRKFGAEWYNQVEITAVELAKAKQSGHKF"
     gene            complement(2281..2928)
                     /locus_tag="PKH68_03160"
     CDS             complement(2281..2928)
                     /locus_tag="PKH68_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNX11968.1"
                     /translation="MNLSFINFIDYLGTFAFAISGMRLASAKRFDWFGAYVVGLVTAI
                     GGGTTRDLLLDVTPFWMLQPSYLIVTGFALLFVIFLGKWVIRLHNTFFIFDAIGLGLF
                     VVVGIDKALELNYPFWVAILMGMITGSVGGVIRDILINEVPLIFRKDIYALACVVGGL
                     IYFVSFKLNFSPVVMQTTAAVSVIVTRVIAVKYHISIPVLKAYEDFKNEKENDRK"
     gene            complement(2989..3390)
                     /gene="secG"
                     /locus_tag="PKH68_03165"
     CDS             complement(2989..3390)
                     /gene="secG"
                     /locus_tag="PKH68_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNX11969.1"
                     /translation="MYVFLIVLIIIFSLLLTFIVLIQNSKGGGLAAGFQSNNQIMGVR
                     KTTDFLEKATWGLAAVIIVLSVISVGVKSGAPKDVNDSEIKEQSMDLNKQMQQTTATP
                     NFGETAPAQEQQAAPAQETPKQAPAATPEKK"
     gene            complement(3403..4044)
                     /locus_tag="PKH68_03170"
     CDS             complement(3403..4044)
                     /locus_tag="PKH68_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11970.1"
                     /translation="MTYDNLIDKLRQSEKLTGDDAPALRKLVDLYPYFGVGRWLYLKA
                     LYNSNSLFFGAELKRTALHAPNRRNLYFFIHPEELKTVGQKERTGNSTGSYFDMLEAV
                     ENKNKNNPNSLRALAERLKAAREMLHTGIPEEHSAACTQEVENLSQEEQQQTMPGSDL
                     SDEQRELEVKKCIREKKYREAIELLKELNFNNPKKSIYFADQIRYLEKILQNL"
     gene            complement(4061..4609)
                     /locus_tag="PKH68_03175"
     CDS             complement(4061..4609)
                     /locus_tag="PKH68_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNX11971.1"
                     /translation="MALIYDILNLIMIKKLKISLGLCAVALILQGCAVTYGFKSGSID
                     YTKTKSISITDFPNNADLVNPTLSQTFVEALKDKYTRSTRLQLLSRGGDMHLEGEIVG
                     YQISSMAISADSYASQTKLTLTINVRFTNNKKPEDDFETRYSAFQMFNSDRMLNDVEP
                     ELVKTMVDEIVDKIFNDSVAKW"
     gene            complement(4593..5918)
                     /locus_tag="PKH68_03180"
     CDS             complement(4593..5918)
                     /locus_tag="PKH68_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNX11972.1"
                     /translation="MKPKELQAVKQRFGIIGTSELLDRAIDIAIQVAPTDLSVLITGE
                     SGVGKENFPQIIHNYSHRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGATADRKGY
                     FEVADGGTIFLDEVGELPLSTQVRLLRVLEAGEFIKVGSSKTQKTDVRVVAATNMNII
                     QATRDGRFREDLYYRLNTVPIFVPPLRDRKEDIPLLFRKFAADFAEKYKMPPIRLSDD
                     ARLMIMNYYWNGNVRQLKNITEQISVIEQQRDITPAILRSYLPVEDMERLPAIFQREN
                     SDHKTFQNEREILYQVLFDMKKDMIDLKKIVNELSMGRSVNVQPVDTDFSTGFFHNDN
                     ILNQNPNQSFSPDNTPFKKFSHADDVETFHEVEEYEDADADEEEYKEKLEKLFDAADD
                     GKYTMAEMECEMIKKSLEKNHGRRKLAAKDLQISERTLYRKIKDYGIDL"
     gene            6176..8134
                     /locus_tag="PKH68_03185"
     CDS             6176..8134
                     /locus_tag="PKH68_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNX11973.1"
                     /translation="MNKEHKVPFGLMGLFLAVGIVYGDIGTSPLYVMKAILSGLPAGQ
                     KANPEFIYGAISCIIWTLTLQTTVKYVLITLRADNHGEGGILSLYALIRKNYRWAYFI
                     AAIGASTLLADGVITPAITVISAVEGLNALQPALPILPITIAILVFLFLIQPLGTSRL
                     GKTFGGIMFLWFTMLGALGIYALLSFPHVLAAFNPVYAVKLILASPNALIILGAVFLC
                     TTGAEALYSDLGHCGLQNIRISWIYVKTTLILNYLGQGAWIITHPNNLYEGVNPFFGM
                     MPSWFSFIGVAMATLAAIIASQALISGSFTIISEAVSLDLWPNIHIKYPTEIKGQLYI
                     PLVNHALMVLCIIVVLFFASSSNMEAAYGLAITITMMMTTVLLFIYLYNSKKPLWFSI
                     PVTLFFITLEGGFFTANMLKFVHGGYVTIVIAGGLFFIMYVWYNGRRIKNRSVSYIPI
                     ARHIPQIDKISNDREVPKFATHLVYVTRAKYADEMESKIIQSIINKQPKRADTYWFVY
                     LTRSDEPYEFSYEVKTFIPGKIFRVNIRAGFKMGIHVDTYVRRICKEMEKKGEVDLTS
                     RYPSLKEANIEGDWRFVVVERIVRNMDLPTISRAILSMYYGIKKFTTSDTQILDLDPS
                     VVTVEYVPLKNLRPAPRKDQPYFTFEEE"
     gene            8305..9408
                     /gene="ald"
                     /locus_tag="PKH68_03190"
     CDS             8305..9408
                     /gene="ald"
                     /locus_tag="PKH68_03190"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNX11974.1"
                     /translation="MKIGIPKEIKNNENRVSLTPSGAYTLVLRGHTVYVQKSAGVNSG
                     FSDERYIKAGAQILPDAESVYAEAEMIMKVKEPIESEYKLIRPNQLVFTYFHFASDEK
                     LTRAMIDSQAVCLAYETVEIGRTLPLLFPMSEVAGRMSVQEGAKYLEKPKGGKGILLG
                     GVPGVKPANVLVIGGGVVGQNAALMAAGLGARVTICDISLPRLRYLSEIMPANVVTLM
                     SSKYNIEEILPSTDLVIGAVLIPGAKAPYVITKDMLKLMEPGSVLVDVAIDQGGCFET
                     SHPTTHAEATYKVDEIVHYCVANIPGAVPFTSTLALTNATLPYALLLADMGWETACGI
                     YPDLRLGLNIVNGKVVYKAVADTFGLPYEELTF"
     gene            complement(9490..>9810)
                     /locus_tag="PKH68_03195"
     CDS             complement(9490..>9810)
                     /locus_tag="PKH68_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNX11975.1"
                     /translation="IINDVTEDKSINIGDIEITNKFTFFDVFEDQKDKVLNSFRHAED
                     MEVSVAKGSKSGGDYSDRGKRDFSSKERKSSGGGNYRNEEKPWRNRDTKNRGNDKKKR
                     WKTA"
     assembly_gap    9811..9910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9911..11025)
                     /locus_tag="PKH68_03200"
     CDS             complement(<9911..11025)
                     /locus_tag="PKH68_03200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX11976.1"
                     /translation="MTFKELNLKDGILSAIEDLGYEQPMPVQEQVIPFMLTQSADLVA
                     LAQTGTGKTAAFGLPILNMIDVDRHQVQALVLAPTRELCIQISNDLKGYSKNLDGLRI
                     VPVYGGEDIRVQLRQLDQAPQIIVATPGRLIDLIERGKIKLQNINFLVLDEADEMLNM
                     GFKEDIEKILEQTPDTRRTTLFSATMPAEIQRIARQYMHDYKEITVGSRNAGTENVEH
                     IYYVSQARQRYLVLKRIVDLNPDIYGIVFCRTRQETKEVADKLMQDGYNADALHGDLS
                     QSQRDTVMLKFRVRNIQLLVATDVAARGLDVSDLTHVINYNLPDDVEIYTHRSGRTGR
                     ANKTGVSVSIIHSKEKFKIKDIERMLKRKFEQQQIPNG"
     gene            11428..13074
                     /locus_tag="PKH68_03205"
     CDS             11428..13074
                     /locus_tag="PKH68_03205"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNX11977.1"
                     /translation="MTKSPLQIARANYQPKLPAALLGNVAVVEGAATESVADQADIKN
                     LFPNTYGMPLLKFEPGEKKTYPQVNVGVILSGGQAPGGHNVISGLFDGLKRLNPANKL
                     YGFLDGPIGLVDHKYIELTEKGVADYRNTGGFDIIGSGRTKLEEEWQFDKGVEICQKL
                     GIKAIVIIGGDDSNTNACVLAEYYKQKDAGIQVIGCPKTIDGDLKNEMIETSFGFDTA
                     CKVYSELIGNIQRDANSAKKYWHFIRLMGRSASHITLECALQSQPNICLISEEVEAKN
                     MTLDDIVNNIVKVIVNRGNAGLNFGTVLIPEGLIEFIPAMKKLIAELNDMLADNDDFN
                     AMNTDDERREYVKGMLTPDSCNIYRSLPKGIAKQLTLDRDPHGNIQVSLIETEKLLIE
                     MVGKRLAQMKAEGNYKGKFSAINHFFGYEGRCAIPSNFDADYTYSLGYTASVLISEGK
                     TGYMASVRNLTEPAAEWIAGGVPITMMMNMERRSGKMKPVIQKALVRLDGEPFKFFAT
                     YREKWEDAQLDYIYPGPIQYFGPTEVCDQPTRTLLLEQGK"
     gene            13378..>13883
                     /locus_tag="PKH68_03210"
     CDS             13378..>13883
                     /locus_tag="PKH68_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX11978.1"
                     /translation="MLKFFTDNRGYILCLLTAATLFTSCVTAKKTNYLQNPSIYIPKY
                     DNSVGYEEYKLAPSDKLYVRMYSTEKETNRLFNGSNQMMTSMMGGSSDYTDLYTYTIK
                     NDGTVKLPIAEESIYLAGRNIRETKKIIEDALRPYIKEEFVVDVKIIGRYFSVIGGGS
                     NGKFPILR"
     assembly_gap    13884..13983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14071..14862
                     /locus_tag="PKH68_03215"
     CDS             14071..14862
                     /locus_tag="PKH68_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNX11979.1"
                     /translation="MPQILNKDRNGQLKLVRLTLLAGLVLILNSCYTPGNTLLLQNSK
                     ALPNYPKAAFEDYKIQVNDEVMFRLITSNENFASLIDAGNVASSQQSNSYRVYPDGTI
                     DLPFMDSIPVAGLTFTQAARSIEKRFKDLIPDAEVNLTLANKSYTVIGEAGTGIFPVY
                     KDRLTIFQALAQSGEIQQSGDRRHVKILRETSGGPAIMEFDIRPTSIIDSKYYYIYPN
                     DIIYVRKQTSSFYKVNNYGSFLGLIMSSVSLFINVFYYTKLTKSN"
     gene            14904..>15367
                     /locus_tag="PKH68_03220"
     CDS             14904..>15367
                     /locus_tag="PKH68_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11980.1"
                     /translation="MEENYYYNKQKFNDEEESGFDIKEWISLFLHFWYLFIIFGLLAL
                     GLAYLKNRSLIESYQSSGTIIIEESRYAGGSQALMQGFGVKSGYSNVDNQIVMLTSYD
                     LINRVVDSLPQMKVDYISKGRFKTRNLYNTSPIVINTDYIDSKAYGALFKISL"
     assembly_gap    15368..15467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15468..17105
                     /locus_tag="PKH68_03225"
     CDS             <15468..17105
                     /locus_tag="PKH68_03225"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNX11981.1"
                     /translation="KLSETFLAENLERKNDAATKTMRFIDEQLDAVSKSLSVSEGAMT
                     EFRRSNKIVDLPSHSSEVLGKATQYDEKQSELKLRETYLNYLTDYLKTNMASGAVVAP
                     SSLGLNEPMLMALVQQFNELISERNKTSERNPLYAKNEREIANLKLSINEVVKNMRAS
                     MNIEKTDLNNKLASVQKEINSLPDKEMEMISIERKYRVDDNYYTFFLQKRAEAAIQKA
                     SNSPDNNILDKARIITQINGDKKSKTTMVYLLIGLLIPAIFIVLKEVLNNTIRNSKDV
                     EKNGAFTLIGAIRHTHSTDPILAGIRPRSSFTEMFRVIRTRIEFIVQRKSKIAILATS
                     TESGDGKTYFCTNLASVYALTGLKTLLIDMDIRKPSVNNRMGITEKNGVTNYLIGEFE
                     LNDLILKKDGIPFDILLAGTVPPNPGELIRSDKLKEMFSQLRLIYDYIIVDSSPIGLV
                     ADAYSLAPLMDTNLMIVRSEKTNKSFFKRLNIQLKADNMSNMYTIINDVKLDGNSYSK
                     YSSSYGYGYGYGYGYGSGEGKKKGNSYTHYYEDDSDI"
     gene            complement(17196..17528)
                     /locus_tag="PKH68_03230"
     CDS             complement(17196..17528)
                     /locus_tag="PKH68_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11982.1"
                     /translation="MNQRILNIAQIAGAILTFGGALIKLLEPGTPGTYIFTAGAVILL
                     VVQFINVLKTKNADMRIKRLNRILLLATAVLGVAVYMMFANPVSESWVVMVLLYAVLS
                     VFLTFRAN"
     gene            complement(17525..17836)
                     /locus_tag="PKH68_03235"
     CDS             complement(17525..17836)
                     /locus_tag="PKH68_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNX11983.1"
                     /translation="MTTNQHKFSRFRRLLLNKYVVTLVGFFIFLIFFDNHNLISRWET
                     SRKINSMEKEIKFYQSEIDKNKAKMNEMQTSDESLEKYAREQYHLKKDSEDVFIIKEE
                     E"
     gene            complement(17926..>18318)
                     /locus_tag="PKH68_03240"
     CDS             complement(17926..>18318)
                     /locus_tag="PKH68_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNX11984.1"
                     /translation="MLPVGFLVEELEKAQFASAFGRFCRSEILLLLANDYGSKAATNA
                     GRMKELFDKFDFRYRQEKASTDSFSVEKEAVARAQREGYGVVIISASRDYGLDDLLLG
                     PKELHLVRKSTVPLLLVNPRGDLYTLCD"
BASE COUNT         5167 a         3942 c         3711 g         5198 t
ORIGIN      
        1 ttatggctgc atccagacca attttattga tggtacgtaa tccggcagcc gaaattttga
       61 ggctgatcca catatcctgt tctacccaat agaacttttt ggtgaacaag ttcacatcaa
      121 aagtacgttt ggtatgacgt tttgagtgtg atacgttaca tccacccatt gcttttttac
      181 cggtaatttg acaaattttt gacatcgctg tatctcttta atttttatct ttattttttc
      241 tcaaaaacag tgtgcaaaag tatggttttt ttttctttca cacaaatttt ttctgcttat
      301 atttcaattt ttctgacaat taacaaaaat aaagtattca aggcatatat tagctaagaa
      361 aaacgaatgc tttactaaat tcttcgattt tttcaaatca tttcattgag catcatcagt
      421 gccgccatag tgcttctttc tataaaattt tcgcgtgcga actgtccaaa attgaaactt
      481 cgggtaatgg ttcgttccgg cgaacttacg gatatccaga tggtacctac gggtttttcg
      541 taagttccac cggtgggtcc tgctataccg gtagtggcta ctgccagatc agtttgcatc
      601 actttacgtg ctccttcagc catttgagtg gctacctgtg aacttaccgc tccatactgc
      661 tcaatatcct gtgccgatac acccagcata ttgattttca attcgttgtg ataggccact
      721 accgtacctt tgaagcagtc tgaaatgccc ggtattaacg taagccgatg tgctatattg
      781 cctccggtac aactttcggc ggttgaaact gttagtcccg aacttttcag tttttcgtag
      841 atgatctttt caaccggttt atcttcgtaa gcaaaaatac tttctcccaa aattgattta
      901 agggtttcaa cctgctggtt aatagcctct tccagtttat atttatcctg accagcacca
      961 cttatccgca acttaacgat accaaaattc ggtaaataag ccagatgtaa atattcgggc
     1021 agcgaatttt cccattcggt aagacggagc gcgagctgag actcgggaat tccatacacc
     1081 tgaacagttt tatgaatgat agcgggtgtt ttgaaatgct tttgcaggcg gggaataatt
     1141 tcttcgctca tcactttttt catctccagc ggcacgccgg gcatcgacac aatcactttt
     1201 ccctcttttt cgaaccaggt aatgggtgca gagccggccc tgttctgaat tacggtgcac
     1261 ttttcgggta ccattgcctg cgatttggtc agttcattca ttacagactg gcgggtatgg
     1321 taaatttgtt gaatattttc gagtacttcg ggattaaata ccagttttgt tccgaaatat
     1381 tcacacaggg tctgtttggt aatatcgtct ttggtgggtc caagcccgcc ggtcataagc
     1441 accacatctg cacgaagcaa agcttcatcc agtgcttttt tgatgtgatc agcctcatcg
     1501 tgtacagatg taatctgtgc cagctgaaat cctgctttgt tgagttccac ggccatccag
     1561 gctgaattgg tatcaacaat ctgacctatc agtatttcat cgcctatggt aataatttca
     1621 atcatgttac cctccctaaa atcctgaaat acaagattta ttcaagaatt ttgttttgtt
     1681 atatatgatt ttaaaatttg tgtccagatt gttttgcttt ggctaattct acagcagtta
     1741 tttccacctg attgtaccat tctgcaccaa atttgcgaat taaaggttct ttaaggaact
     1801 tatacaccgg cacgcccaga ttttttccct gaacacgggc acattcgcac acaatccatt
     1861 tatgtacatt tactgccgta aactctttat ttttctgcag ccgtacggga tacaaatgac
     1921 aggagatcgg cttgtagaag tcagttttcc cttcgcggaa agctttttct atggcacact
     1981 tacatattcc ctgcccgtcg gtgtaagtaa agacacattc gcgtccgttg acaatcgaag
     2041 taaccggctc atcctcctga tcaagataaa aaacaccttg ttttttaatc agattttgag
     2101 atgccggcga taaatcatcc caaatttgag gcaggatttc ctcgagaata tcaatctctg
     2161 tccaatcgag cggagcgccc gaatcgcctt ctatacagca tattcctttg cacgatgcca
     2221 ggtcgcagac aaatttttcg tcgaacaaat caaaacttaa tatcgtatca tcaattaaaa
     2281 tcattttcgg tcgttttctt tttcattttt gaaatcttca taagctttca gtacaggtat
     2341 gctgatatga tattttactg ctatcacacg cgttacaatt accgagactg cagctgttgt
     2401 ctgcatcact acgggtgaaa aattcagttt gaacgacaca aaataaatca atcctccaac
     2461 cacacaagcc agtgcataaa tatcttttcg gaaaatgaga ggaacttcat tgatcaatat
     2521 atcgcgaatt acgcccccga ccgacccggt aatcataccc atcaatatgg ctacccaaaa
     2581 gggatagttg agctcaagcg ccttgtcgat gcccaccact acaaacagtc ccaacccgat
     2641 ggcgtcaaat ataaaaaaag tattgtgtag ccggatgacc cattttccta aaaaaataac
     2701 aaacagcagt gcaaaaccgg ttacaataag atacgatggc tgcaacatcc agaagggagt
     2761 gacatcgagc agcaaatcgc gggttgtacc gccaccgatg gctgtaacca atcccactac
     2821 ataggctcca aaccagtcaa atcgtttcgc cgaggccagt cgcatgccgc ttattgcaaa
     2881 tgcaaaagta cctaagtaat cgataaaatt tatgaaagac aagttcatac gataaaaaaa
     2941 agcgtatcag aaattttctc gatacgcttt tgaaggggtt atttcctttt atttcttttc
     3001 aggcgtagca gcaggtgctt gtttcggagt ttcctgagcc ggagcagctt gttgttcctg
     3061 tgcaggagca gtttcaccaa agttgggtgt agctgttgtt tgctgcattt gcttattaag
     3121 atccatcgat tgctctttta tttcggagtc attcacatct ttaggtgcac cggattttac
     3181 tcctaccgat ataacagaca aaacgatgat tactgccgcc aatccccagg ttgccttttc
     3241 cagaaagtca gtggttttgc gaacacccat aatctgatta ttagactgaa aacctgctgc
     3301 caggccacca cctttggaat tctggattaa aacaataaaa gtaagtaaaa gtgaaaaaat
     3361 gatgattaat acaattaaaa aaacgtacat aatgagttta ttttataagt tctgtaatat
     3421 tttttctaaa taccgaattt ggtctgcaaa gtaaatactt tttttcggat tattaaaatt
     3481 taattctttc aataattcaa ttgcttcccg atattttttt tcgcggatac attttttgac
     3541 ttcgagctcc cgctgttcgt cgcttaaatc cgaacccggc attgtctgct gttgctcttc
     3601 ctgtgacagg ttttcaacct cttgagtaca cgctgcggag tgctcttccg gtataccggt
     3661 gtgaagcatc tcgcgggctg ctttaagccg ctcggccagt gcacgcagtg aatttgggtt
     3721 atttttattc ttattctcga cagcttcgag catatcgaaa tagctgcctg ttgaatttcc
     3781 agtccgttcc ttctgtccca cggtttttaa ttcttcggga tgaatgaaaa agtaaagatt
     3841 tcggcgattt ggtgcatgca gggctgttcg tttcagttct gctccaaaaa acaggctatt
     3901 ggaattgtac agcgctttca gatataacca ccgccctacc ccgaaatacg ggtacaaatc
     3961 aaccagcttc ctgagagcag gcgcatcatc accggtcagt ttttcggatt gacgaagttt
     4021 atctattaag ttatcgtaag tcataggccg ttcagcagga ttaccatttg gctaccgaat
     4081 cattaaatat tttatccaca atttcgtcca ccatggtttt gaccaactcg ggctccacat
     4141 cgttaagcat ccgatcgctg ttgaacatct gaaaagcgga atatctcgtt tcaaaatcat
     4201 cttcaggttt tttgttgttg gtaaatcgca cattgatggt cagcgtgagt ttggtctgcg
     4261 atgcgtatga atcggcgctg atggccatgg atgagatctg atatccgact atttcgcctt
     4321 ccaggtgcat atcaccgcca cgggacaaca actgcagacg agtagatcgg gtgtatttgt
     4381 ctttcaatgc ctcaacgaaa gtttgtgaca gagtcggatt gaccagatcg gcattattgg
     4441 ggaaatcggt aatggaaata cttttggttt tggtataatc aatagagccg ctcttgaatc
     4501 cataggttac cgcacaaccc tgtagtatta atgccacggc acacaatccc aacgaaattt
     4561 tcagcttttt tatcattatc aaattcaaaa tatcataaat caatgccata atctttaatt
     4621 ttgcggtata atgtccgttc ggaaatctgc aaatccttag cggccagttt tcgtcgtccg
     4681 tgattttttt ccaacgactt tttgatcatt tcacactcca tttctgccat tgtgtatttt
     4741 ccatcatcgg ctgcatcgaa taatttttca agtttctcct tatattcttc ttcatctgca
     4801 tcagcatctt catactcttc aacttcatgg aatgtttcca catcgtcggc atggctgaat
     4861 tttttaaagg gagtattatc gggcgaaaat gattgattcg gattttgatt gaggatgtta
     4921 tcattgtgaa aaaaaccggt cgaaaaatcg gtatctaccg gctgaacatt tacggaacga
     4981 cccatggaaa gttcgtttac aatctttttc aggtcgatca tgtctttttt catgtcgaat
     5041 aagacctgat acaatatttc gcgctcattt tgaaaagttt tatggtccga attttcgcgt
     5101 tgaaaaatgg ccggaagtct ctccatatct tcaaccggca gatacgagcg aagaattgcc
     5161 ggagtgatat cacgctgttg ctcaatgacc gaaatttgtt ctgttatatt tttcaattgt
     5221 cgaacattgc cattccagta atagttcata atcatcaacc gggcatcatc actaaggcgg
     5281 ataggcggca tcttgtattt ttcggcaaaa tcggctgcaa atttccggaa cagcagcgga
     5341 atatcttctt tgcggtcgcg gagtggcggc acaaaaatgg gtactgtatt taagcgataa
     5401 tataagtctt cgcggaatcg tccgtcgcgg gtagcctgta tgatattcat attggtggca
     5461 gctactaccc gtacatcggt tttttgtgtt tttgaggacc ccactttgat aaactcaccg
     5521 gcctcgagca cgcgcaggag tctcacctgt gttgacaggg gcagttctcc cacttcatcc
     5581 agaaatatgg ttcctccgtc ggcaacttca aaatagcctt tccggtcggc tgtagcacct
     5641 gtaaatgagc ctttttcatg tccgaaaagt tcggagtcaa tggttccttc aggtatggcg
     5701 ccacagttga ctgcaatgta aggaccgtgt ttgcggtggc tgtagttatg tattatctgc
     5761 gggaaatttt ctttccccac accgctttcg ccggtaatca gaacagataa atcggtagga
     5821 gctacctgaa tagctatatc gatagcccga tcgagtaatt cggaggttcc aataatacca
     5881 aatcgctgct taacggcttg taattcttta ggtttcatat caagttccac atttttgcac
     5941 tgttaatcat ctgacaagaa aaattaagtg acaaaattga caaattagct gacaaaatta
     6001 caaaagtttt tccaaaacct ttctggttat aggttaaaaa tagttttagg taaatctgaa
     6061 caaaccttct aagatataaa gtctcgattt ttttcaaggt tttcctcctt acctgataaa
     6121 aaacaaagaa tattaaataa atttgcacgt tttaaaaata ctcatttttt agcaaatgaa
     6181 caaagaacat aaagtgcctt tcggcctgat ggggctattt ctggcggtag gaatagttta
     6241 tggcgacatt ggcacctcgc cgctctatgt gatgaaagcc attctcagtg gtttgcctgc
     6301 aggccagaaa gccaatcccg aatttatcta cggtgccatt tcctgcatca tctggacgct
     6361 taccctgcaa accaccgtaa aatatgtgct tatcaccctt cgtgccgata atcatggtga
     6421 aggtgggata ttatcactat atgcacttat ccgtaaaaat tacaggtggg catactttat
     6481 agcagctatc ggtgcttcca ccctgcttgc cgacggggta atcacaccgg caatcaccgt
     6541 tatttcggcg gtggaagggc tgaatgccct gcagcctgca ttacctattt tacccattac
     6601 catagctatt ctggtattcc tgtttttaat acaaccattg ggaacttcgc gtctgggtaa
     6661 aacatttggg ggcatcatgt tcctgtggtt taccatgctc ggtgcactgg gtatttatgc
     6721 tttgctttca tttccccatg ttttggctgc tttcaatccc gtatatgccg tgaagttgat
     6781 actggcatcg cccaatgcat tgattattct gggcgctgta ttcctctgta ccaccggtgc
     6841 tgaggctctt tattcagatc tgggacattg tggacttcag aacatccgga tttcgtggat
     6901 ttatgtgaaa accacactta tattgaatta tctgggacag ggtgcatgga taatcaccca
     6961 tcccaataac ctctacgaag gtgtaaaccc ctttttcgga atgatgcctt cctggttttc
     7021 atttatcggg gtggctatgg ctacactggc agccattatt gccagtcagg cacttattag
     7081 cggttcgttc accatcatca gcgaagccgt ttcactggat ttatggccca atatccacat
     7141 caaatacccc accgaaatta agggtcaact gtacattccg ctggttaatc atgcactcat
     7201 ggtactatgt attattgtag tgctgttctt tgccagctca tcaaatatgg aagctgctta
     7261 cggactggcc atcaccatca ccatgatgat gactacagta ctgttgttta tttatcttta
     7321 taattcaaaa aaaccacttt ggttcagcat tcccgtcacc ctgtttttca tcaccctcga
     7381 aggtggtttt tttactgcca acatgctaaa attcgttcat ggagggtatg ttaccatcgt
     7441 gattgccggt ggcttgtttt ttatcatgta tgtgtggtac aacggccgta gaattaaaaa
     7501 ccgcagcgtt tcgtacatac ccattgccag acatatacct cagatagata aaataagcaa
     7561 cgacagggaa gtgccgaaat ttgccacaca cctggtatat gtaacccgtg ccaaatatgc
     7621 cgacgaaatg gaatcgaaga tcatccagtc aatcatcaac aaacaaccaa aacgggctga
     7681 tacctactgg tttgtgtacc tcacccgaag cgacgagccc tacgaattca gctacgaagt
     7741 gaaaacattt attccgggta agatattccg ggtaaacatt cgcgccggtt tcaaaatggg
     7801 cattcatgtc gatacgtatg tgcgccgcat ttgcaaggaa atggagaaaa agggagaagt
     7861 ggatctgacg agccgctatc cctccctgaa ggaagcaaat attgaaggcg actggagatt
     7921 tgtggtggtg gagagaattg tgcgcaatat ggatttgcct acgatatccc gagccatact
     7981 ttcgatgtat tacggcataa aaaaattcac cacatccgat acacaaatac ttgatctgga
     8041 tccttcggtg gtaacagtgg aatatgtacc gctcaaaaat ctgcgacccg ctcccagaaa
     8101 agatcaaccc tatttcactt ttgaagagga ataaccggaa cgaattattg gattaaaatc
     8161 agacatttag ttagcaacaa aacgcccaca ttcgccaaat cagaaattat tttgctgaaa
     8221 tctgcaatat ttccggataa atatatcaat ttgtcaaaaa actatcgtat ttttatcccg
     8281 taaaattcat caacatttaa aaccatgaaa ataggaattc caaaagaaat taagaacaat
     8341 gaaaaccgtg tttcattgac tccatcggga gcttacaccc ttgtactgag gggtcatacg
     8401 gtatatgtac agaaaagtgc cggcgtcaac agcggatttt cggacgaacg ttacatcaag
     8461 gccggtgcac agattttgcc cgatgccgaa agcgtatatg ctgaagcaga aatgatcatg
     8521 aaagtaaaag aaccgattga gagcgaatac aaattaatcc gccccaatca gttggtgttt
     8581 acctattttc actttgcttc cgacgaaaaa ctgactcgcg ccatgatcga tagtcaagcg
     8641 gtttgtctgg cttacgaaac ggtggaaatt ggccgaacgc tgccattgtt atttccaatg
     8701 tctgaagttg ccggacgtat gtcggtccag gaaggtgcca aataccttga aaagcccaaa
     8761 ggtggaaaag gtatattgct tggaggagta ccgggtgtta aacctgccaa cgttcttgta
     8821 attggtggcg gtgtagtggg acaaaatgcc gcattgatgg cagccggcct cggtgcccgc
     8881 gttaccatct gcgacatttc attgccaagg ctacgttacc tgagcgaaat tatgcctgcc
     8941 aatgtggtga ccctgatgtc gagcaaatac aatattgagg aaattcttcc ttctactgat
     9001 ctggtgattg gcgcagtact cataccgggt gcaaaagcgc catatgtcat taccaaagac
     9061 atgctcaaac tcatggagcc gggatcggta ttggttgacg tggctattga tcagggtggc
     9121 tgtttcgaga cttcgcaccc cactacacat gccgaagcca cctataaagt ggatgaaata
     9181 gttcattact gtgtggccaa catacccgga gctgttccat ttacatccac actggcgcta
     9241 accaatgcta ccctgcctta tgcattgctg ctggctgaca tgggttggga aacagcttgt
     9301 ggcatttatc cggatctgcg ccttggattg aatattgtca atgggaaagt ggtgtacaaa
     9361 gcggtagctg acaccttcgg gttgccttac gaagaactga cattctaata tcagagttct
     9421 taaaaatcca taaaaacaaa gagcttattc gttgtgaata agctctttgt ttttatttca
     9481 aatcagcggt taggcagttt tccatctttt ctttttatca tttccccgat ttttggtatc
     9541 gcggtttctc cagggtttct cctcgtttcg gtaattgccg ccgccactgc ttttccgttc
     9601 tttggagctg aagtcgcgct tgccccgatc cgaataatca ccacccgatt ttgatccttt
     9661 ggctaccgac acttccatat cttcggcatg cctgaatgaa ttgagcactt tatccttctg
     9721 gtcttcgaat acatcgaaga aggtgaattt gttcgttatc tcaatatcgc cgatattaat
     9781 ggatttgtct tcagttacat cgttgattat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn ccgttaggga tttgttgctg ttcgaacttg cgtttcagca tgcgttcgat
     9961 atccttgatt ttgaattttt ccttggagtg aatgatggat accgaaactc ccgttttgtt
    10021 ggcacgtccg gtacgaccgc tgcggtgggt ataaatttcc acatcatcgg gcaggttgta
    10081 attgatcaca tgcgtcaggt cgcttacatc gagtccacgg gcagccacat cggtagctac
    10141 caggagttgg atattgcgta cgcgaaattt gagcattaca gtatcgcgct gcgattgtga
    10201 caagtcgcca tgcagtgcat cggcattgta accatcctgc atcagtttat cggccacttc
    10261 cttggtttcc tgacgggtac ggcagaaaac aattccgtaa atatcggggt tcaggtccac
    10321 gatgcgtttc agcaccaggt aacgctgacg ggcttgagac acataataaa tatgttcaac
    10381 attttcagta cctgcatttc gtgagcctac agtaatttct ttgtaatcat gcatgtactg
    10441 acgtgcaatt cgttgaattt cggcaggcat ggtagccgaa aacagtgttg tacgtcgggt
    10501 atcgggagtt tgttccagaa ttttttcaat atcttcttta aatcccatgt tcagcatttc
    10561 atcggcttcg tcgagcacca gaaagttgat attttgtaac ttaatctttc ctctttcgat
    10621 gaggtcaatg agtcgacccg gtgtggcaac aataatctgc ggagcctggt caagctgacg
    10681 aagctgcaca cgaatatctt caccgccata aaccggcaca attcgcaaac catccaggtt
    10741 tttggaatat cctttcaggt cgttggatat ttgaatacaa agttcgcggg tgggcgccag
    10801 cacgagtgcc tgcacctgat gacgatcaac gtcaatcatg ttgaggatgg gcagtccgaa
    10861 tgcagctgtc ttaccggttc cggtttgggc aagtgccact aaatcggccg actgtgtcag
    10921 catgaatgga atcacctgtt cttgtacggg cataggttgc tcgtatccga gatcttcgat
    10981 agccgaaaga atcccgtctt ttaaattcag ttctttaaat gtcattttgt tttgtttagc
    11041 acacgtttgg agtagtcatc cgaagcccga tgaaagcaaa accgtaaatc attgttacgt
    11101 gtttcttcta aaaaaaagct ttcaacactc tgttagaata aaaaagaaaa tgtttccgga
    11161 aggaaaacgt gtaccttaaa gatatccatc tgtctgaacg acagcaaaat tcagaatttt
    11221 acccacaacg cgcagttgtt ttttaaatcg ggtgcaaagg tacggaaatt attccgaata
    11281 aaaatacaag tattgaattt tgttattttg tcaaaataga gattataatt ctttcaaata
    11341 ttaatttatt tttctaattt tgcagagaat tatcattaaa tgcaaacaac tgaatatcaa
    11401 acatctaaaa tacaatttat gaaaccaatg acaaaaagcc cgctgcaaat agcacgtgct
    11461 aattaccagc ccaaacttcc ggcagcatta cttggcaatg tggctgtggt agaaggtgct
    11521 gccacagaat ctgttgccga ccaggctgat ataaaaaatt tattccccaa tacgtatggg
    11581 atgccactgc ttaagttcga acccggtgaa aaaaagactt atcctcaggt aaatgtaggc
    11641 gttatccttt cgggtggaca ggctccgggc gggcacaatg taatcagcgg actttttgac
    11701 ggacttaaac ggttgaaccc cgcaaacaag ctgtatggtt ttctggatgg ccccattggt
    11761 ctggtagatc acaaatacat agaattaacc gaaaaaggag tggccgatta ccgcaatacg
    11821 ggtggtttcg atattatcgg gtccggccgt accaagctgg aagaagaatg gcagttcgac
    11881 aaaggggtgg aaatctgcca aaagctggga attaaagcca tagtaatcat cggtggtgac
    11941 gactccaata ccaatgcctg tgtgctggct gagtactaca aacaaaaaga tgccggaatc
    12001 caggtaatcg gttgcccaaa aacaattgac ggtgacctga aaaacgaaat gattgagacc
    12061 tcgttcgggt ttgataccgc ttgcaaagtg tactccgaac tcatcggaaa tatacagcgt
    12121 gatgctaact cggcaaaaaa atactggcat tttattcgcc tgatgggccg ttcggcatca
    12181 cacatcaccc tggaatgtgc actccagagc cagcccaaca tttgtcttat ctccgaagag
    12241 gtagaagcaa aaaatatgac cctggatgat attgtgaata acatcgtgaa agtgatagtg
    12301 aaccgtggta atgccggttt gaattttggt acggtactta ttcccgaagg tttgattgaa
    12361 ttcataccgg ctatgaaaaa actgatagcc gaactaaacg atatgctggc cgataacgac
    12421 gacttcaatg ccatgaacac cgatgacgaa cgccgtgaat atgtaaaagg aatgcttact
    12481 cctgatagtt gcaacatata ccgcagtctg cccaaaggga tagccaagca gctcaccctc
    12541 gatcgtgatc ctcacggcaa cattcaggtt tcactcatcg aaactgaaaa gttgctgata
    12601 gagatggtgg gtaaacgtct ggctcagatg aaagccgaag gtaactacaa aggcaaattc
    12661 tcggctatca accacttctt cggttacgaa ggccgatgtg ccatcccttc caattttgat
    12721 gccgactata cctattcatt gggttacacg gcttccgtcc ttatttcaga aggaaaaacc
    12781 ggctacatgg cttcggtgcg taatcttacc gaaccggcag ccgaatggat tgcaggagga
    12841 gtgcccatta ccatgatgat gaatatggaa cgccgcagcg gaaaaatgaa accggtaatt
    12901 caaaaagcac tggttcgtct ggatggtgaa cctttcaaat tctttgcaac ataccgcgaa
    12961 aaatgggaag atgctcagct tgattatatt tatccgggtc ctatacaata tttcggtcct
    13021 accgaagtat gcgatcagcc aaccagaacc ttgctgctcg aacagggaaa ataatttggc
    13081 taaactaaaa aataatactc cgcagtttac gtttgcagta agttgcggag ttttttttgt
    13141 aaccggtaat ttaatcggtg aaaaatcatt cagacccggc agcaatgctg gaaacgcaaa
    13201 attcggaata ttatccaata ataagtcaat acggttcaaa attctctgaa tttcttatct
    13261 ctttcaaggt agaaagtgaa aaaaatggat tttttacctc atattctttt taagaaaaat
    13321 agatatttcg accaatttga atatctttgc agataaaaca gttaaaatac gtgcttaatg
    13381 ttaaaatttt ttaccgataa ccgaggctat attttgtgtt tactcacggc tgccactttg
    13441 tttacatcct gtgtcacggc aaagaaaacc aattacctgc agaatccctc catttatatt
    13501 cccaaatatg ataacagtgt aggttacgaa gaatacaaac ttgcaccgag tgacaaactc
    13561 tatgtgagaa tgtattccac cgaaaaggaa accaatcggc tattcaatgg cagcaatcag
    13621 atgatgacct ccatgatggg tggttcgtcg gactatacgg atttatatac ctataccatc
    13681 aaaaatgacg gaacagtgaa acttcctatt gccgaggaga gtatttactt agccggaaga
    13741 aatatccgtg aaacaaaaaa aatcattgaa gatgccctga gaccttatat aaaggaagaa
    13801 tttgtggtag atgtaaagat cattggccgg tatttcagcg tgattggagg cggctccaac
    13861 ggtaagtttc cgattttaag agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnctttcca ctgttttttc caccttctcg tttggtttgt ttatttacaa tttatttacc
    14041 ccttctactc ctgcttcaaa ttaaccgaat atgccacaaa tacttaacaa agatagaaat
    14101 gggcaattaa agctggtaag actaacattg ctggcgggac ttgtattgat tttaaactcc
    14161 tgttatacgc ccggcaacac gctgctgttg caaaattcaa aagcgctgcc caactacccg
    14221 aaagctgcat tcgaagatta taaaattcag gtgaacgacg aagtgatgtt ccgccttatt
    14281 acttcgaatg aaaattttgc ctcactcatt gatgcaggca atgtagcttc ctcgcagcag
    14341 tcgaactcgt acagggtgta tcctgatggt accatagatc tgccgtttat ggattcgata
    14401 ccggtggccg gacttacatt tacccaggct gcacgctcca tagagaaacg atttaaggat
    14461 ctgatacccg atgctgaggt taatcttacg ctggcaaata aatcatacac cgtaataggt
    14521 gaagccggaa caggaatatt tccggtatat aaagaccggt tgaccatatt tcaggcactg
    14581 gctcagtcag gcgaaataca acagagtgga gaccgtaggc atgtgaagat tttgcgtgaa
    14641 acctccgggg gacctgccat catggaattt gatatcagac cgacctctat tattgactca
    14701 aaatactatt atatttaccc caacgacata atctacgttc gcaagcaaac atcgagtttt
    14761 tacaaggtaa ataactatgg gtcattcctg ggattgatta tgtcatccgt ttctttattt
    14821 attaatgttt tctattatac caagttaact aaatccaatt agcttaaagc ctattatttc
    14881 cacacaaata aatatccctg attatggaag aaaattatta ttataacaag caaaagttca
    14941 acgacgaaga agaatctggt ttcgatataa aagaatggat ttcactattt ttacattttt
    15001 ggtatctgtt tatcattttt ggtttgcttg cattggggct ggcatatctt aaaaaccgtt
    15061 cattgataga aagttatcaa tcaagtggta ccattattat tgaagaaagc aggtatgcag
    15121 gcgggtctca ggccttgatg cagggttttg gcgttaagtc ggggtatagc aatgtggaca
    15181 atcagatagt aatgctcaca tcatatgatt tgatcaatag agttgtcgat agtttgcctc
    15241 aaatgaaagt agattacata tccaaaggcc ggttcaaaac cagaaatctg tacaacacct
    15301 cacccattgt tattaatacc gattatattg acagcaaagc gtacggtgca cttttcaaaa
    15361 tcagcctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa ctcagtgaaa
    15481 cattcctggc cgaaaacctg gaacgaaaaa atgatgctgc caccaaaacc atgcgattca
    15541 ttgatgagca gctggacgcc gtttccaaat cgctgtctgt gtcggaagga gccatgaccg
    15601 aattccgacg ttccaacaag attgtggacc tgccgagcca ctcgagtgag gtgctgggaa
    15661 aagccactca gtacgacgaa aaacaatcgg aactaaaact tcgcgagacg tacctgaact
    15721 acctcaccga ctacctgaaa acaaatatgg cttcgggagc tgtagtggcc ccttcgagcc
    15781 tcggtcttaa cgagccaatg cttatggcgc tggttcaaca attcaatgaa ctgatatcgg
    15841 aacggaataa aaccagcgaa agaaatccgt tgtacgctaa aaatgaacgc gaaattgcca
    15901 atttgaaatt atccatcaac gaggtggtaa aaaacatgcg ggcttcgatg aatattgaaa
    15961 agacagacct gaacaacaaa ctggcaagtg tgcagaaaga aatcaattca ctgcctgata
    16021 aagaaatgga gatgatatcc atcgaacgga aataccgggt tgatgacaat tactatacat
    16081 tcttcctgca gaaacgcgcc gaagcagcca tacaaaaggc ctccaacagt cccgacaata
    16141 atattctgga caaagcacgc ataatcacgc agatcaacgg ggataaaaag tccaaaacta
    16201 cgatggtata tctacttata ggacttctca ttccagccat attcattgta ctcaaagaag
    16261 tgctcaacaa caccatacgt aactcaaaag acgtggaaaa aaacggggca ttcaccctga
    16321 tcggtgccat ccgtcatact cacagcaccg acccgatttt agccggtata agaccacgct
    16381 cttcgtttac cgaaatgttt cgcgtaatac gtacccgcat tgaattcatt gtacagcgca
    16441 aatcaaaaat agccattctg gccacctcca ccgagtcggg cgacggaaaa acctatttct
    16501 gcaccaacct ggcatcggtt tatgcgctca caggactgaa aactttgctc attgacatgg
    16561 atattcgcaa gccgagcgta aacaaccgca tgggaataac cgagaaaaac ggggtaacca
    16621 attatctgat cggtgaattt gaactgaatg atctgatact gaaaaaagac ggcattcctt
    16681 tcgacatact actggcaggc actgtgcctc ccaaccccgg tgaattaatt cgttccgata
    16741 aactgaaaga aatgttttct caactaaggc tcatatacga ttatatcatc gttgactcaa
    16801 gtcctatcgg actggtagcc gacgcttact cgctggcacc gctcatggac accaacctga
    16861 tgatagtgcg cagcgagaaa accaacaaaa gtttcttcaa aagactcaac atccaactca
    16921 aagcagataa tatgagcaat atgtacacca tcatcaatga tgtgaaactc gatggaaatt
    16981 catattccaa atattcaagc agttacggtt atggctatgg atacggctac ggatacggta
    17041 gcggcgaagg caagaaaaaa ggtaattcct atacccatta ttacgaggac gattcggata
    17101 tttgacagca gagagaaaat ccggtataaa tacagcatga gggcaattca gaaatccgaa
    17161 ttgccctcat gctgtattat ttgagaataa aaaaatcagt tggcacggaa agtgagaaat
    17221 accgaaagga cggcataaag caacaccatc accacccagg attccgagac cggattggca
    17281 aacatcatgt aaactgcaac acccagtaca gcggtggcta agagtaaaat ccggttcagg
    17341 cgcttgatgc gcatgtcggc atttttggtt ttcaggacat tgataaactg cactaccaat
    17401 aaaataactg cacctgctgt aaatatgtac gttcccgggg tgcccggttc aagtaatttg
    17461 atcaaagctc cgccaaatgt aagtattgca ccggcaattt gagctatatt gagtattcgt
    17521 tggttcattc ttcttctttt atgataaaca catcttcact gtctttcttc aaatgatatt
    17581 gttcgcgggc atatttttca agactctcat cgctggtctg catttcattc attttcgctt
    17641 tgtttttatc aatttccgac tgataaaact taatttcttt ctccatactg ttgattttac
    17701 gcgaagtttc ccaccgactg atcaaattat gattatcaaa aaatatcaga aaaataaaaa
    17761 atccaaccaa cgtcaccacg tatttattca ataaaaggcg gcgaaaccgg gaaaatttat
    17821 gttgattagt tgtcatgcgt aattttttca gatgggaggt acaaagatat aaatttttgt
    17881 attggaaatc agaacaagaa ctgttaaaaa taacaaggta aaatatcaat cgcacaaggt
    17941 atataaatca ccccgtggat tgaccagaag caggggcacg gtggactttc ggactaaatg
    18001 caactctttg gggcccaata agaggtcgtc cagaccgtaa tcacgggagg cggaaatgat
    18061 aaccactcca tatccttcac gctgagcccg ggctacagct tccttctcca cactgaagct
    18121 atcagtgctc gctttttcct gcctgtatcg gaaatcaaat ttatcaaaaa gttctttcat
    18181 cctgccggca ttggtggctg ccttagagcc ataatcattg gccaggagca gcagtatttc
    18241 tgacctgcag aaacggccaa aagccgaagc aaactgagct ttttcgagtt cttcgaccaa
    18301 aaaacccacc ggcagcat
//
LOCUS       DAOPZK010000031        18210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3216_length_18210_cov_24.223960, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000031 DAOPZK010000000
VERSION     DAOPZK010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18210
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3216_length_18210_cov_24.223960"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /gene="lpxK"
                     /locus_tag="PKH68_03245"
     CDS             complement(<1..650)
                     /gene="lpxK"
                     /locus_tag="PKH68_03245"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNX11985.1"
                     /translation="MLKPGKNIIFYILSLIYGSLTTIRNCFFDWGILKQHSFDMPVIS
                     IGNLAVGGTGKTPHTEFLIRQLSEKFRTAVLSRGYRRKTTNFVLADTNSTAQSIGDEP
                     FQIFGKFPQVTVAVDEKRVRGIQNLLKIHPDVQVIILDDGFQHRYVKPGLSILLTDYA
                     NLYTDDYMLPYGTLREFKTNSKRADMVVITKCPEETQPDECKSIAAKLALKPHQKL"
     gene            complement(790..2550)
                     /gene="sppA"
                     /locus_tag="PKH68_03250"
     CDS             complement(790..2550)
                     /gene="sppA"
                     /locus_tag="PKH68_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNX11986.1"
                     /translation="MKQFLKFTLATIVGIFITSLLGFLLLFGFIGAIASTSEKTVKVD
                     PNSVYQLDLEGQLIERSQDDPFSGIFSDAIGRNEVKNLGLDEVLANIKKAKTDKNIKG
                     IYLKGGTLMGGYASVKEIRDALLDFKKSGKFLVAYADTYSQKMYYLCSAADKIYLNPQ
                     GMIEFQGIAANTMFLKNTLDKLGVEAQVVKVGTFKSAVEPYVNTKMSDANKEQVTAFI
                     GSIWNNLLTEISDGRKIHKDTLNRYADEMMTFAPAEKLLQYRMVDSLTYIDGMENVLK
                     KMMKVDKDDKLETINQSDLARVPASVKMEKEKIAVIYAVGGIDMGDTEGIISKDLVET
                     IRKVGDEKSVKAVVFRVNSGGGSAYGSEQIWHALSELKAKKPVIVSMGDYAASGGYYI
                     SCMADSIVAQPNTLTGSIGIFGVIPNIKGLNDKLGLTYDGVKTNKLSDAIDFNRAFTP
                     DERNIMQNYVNRGYEIFVKRCADGRKKTPDQIKAIAEGRVWTGEDAKKIGLVDKIGGL
                     NDAIAMAAKKAKISNYMIREYPEKESFMTRIMKDFNSTIETRIMEGQLGEKYEVLKQI
                     KRLEKFNGIQAVLPFEITTN"
     gene            complement(2652..2948)
                     /gene="trxA"
                     /locus_tag="PKH68_03255"
     CDS             complement(2652..2948)
                     /gene="trxA"
                     /locus_tag="PKH68_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX11987.1"
                     /translation="METFNEIINADKPVLIDFYATWCGPCKTMAPIVEQIGKEVQGKA
                     RVLKIDVDKNPALAARYQIQAVPTLMIFKNGTVVWKHPGGLDKQNLKNALLAHA"
     gene            complement(3039..3473)
                     /locus_tag="PKH68_03260"
     CDS             complement(3039..3473)
                     /locus_tag="PKH68_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNX11988.1"
                     /translation="MSLVRFSQTPWFDRFFDNDLLDWSNRNFSQTNTTLPSVNIKEND
                     DAFMVEVAAPGMDKKDFKIEVHNDLMTISSEKQEENETKNEKEHYTKREFSYQAFTRS
                     FTLPATANGDNVSAAYDKGILLVTIPKKEEAKPKPARMIEIK"
     gene            complement(3776..4498)
                     /locus_tag="PKH68_03265"
     CDS             complement(3776..4498)
                     /locus_tag="PKH68_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11989.1"
                     /translation="MRKIILYILLTIWISSAFGQSGESDYKFSIGDKSLIITSGFSLP
                     SFDIGGKLWEKQPGDLSPTVNFGFRYLLPANFGIKLFGEFTTYADDDKGTKNDTRGGG
                     GKYKFSNQIYRGGARLEYHILGNVTDWEARHNVYVFGGGGVAYATSTVVGDPDKDHFV
                     NTTPVTTPLLLTGIGYQYRLSRNFAVGLDLSYEYYFSDYVEGFTTSWSKSNDVGYGAM
                     FTFSYYFTRIYFQTKKCRCDVD"
     gene            complement(4678..5418)
                     /locus_tag="PKH68_03270"
     CDS             complement(4678..5418)
                     /locus_tag="PKH68_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX11990.1"
                     /translation="MKKLIIVSIFLLFIASVFPQGKGLLTQMPLGEFSLFGGVGPNKL
                     SGDIGGDNFKSLFQNDMGYGLSLGARYTFKYNFALRVFGDYSGYRGKDKVEYNTYGAV
                     RKLEFQSKVTGLGGQLEVVLFGNPLAKDPIPHSVYLLAGLKTTKVDATLINPENNANG
                     PEETKNPLTHFLGIGYQYRFSNQFSFGVEAKQNYFHSDRVDGYNPVIRANKHRDEAFD
                     LKLTAAYYFPFSRRMSEFNNRWDRRQRR"
     gene            complement(5553..7220)
                     /locus_tag="PKH68_03275"
     CDS             complement(5553..7220)
                     /locus_tag="PKH68_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNX11991.1"
                     /translation="MDKTVTIYCKNTQNYHDFPLGTSLLEIYNSLSINLKYQVVAARV
                     NYKVEDLNFLIYKPKDIEFIDTSTSSGMRVYVRSLSMVLSKAVSELYPHTEIRIEHPI
                     SKGYYCRIEHLNEPLTTDIVNRIKAKMREIISRNEPIICEEQQAEHVKKLFRNGTNNT
                     SKDTLFESLGDPYLRYFRIGNFIDYYTSILLPSTGYLNIFDLIPFYDSMLLQVPDRDN
                     PDQLEDMVLQPKMFEIFKEYLGWNRIMDLKSVGEFNRACQNNQAFNLIKIAEALHEKK
                     VASVADMIQARPTVKVVMISGPSSSGKTTFSKRLSIQLMVSGLKPKTISLDNYFVDRE
                     HTPRDENGDWDFEALHALDLPFFNQQLSQLMNGEEIALPSYSFEDGKRHFKGEKLRLE
                     EDTILIMEGIHALNPELIQSIPQDAIFKIYVSALTTISIDEHNWIPTADTRLLRRIIR
                     DYRYRGYSARDTISRWPSVRRGEEKWIFPYQENADVMFNSALIFELAVLKRHVEPILE
                     QVPKYCDEYTETHRLMKFLRYFTAIPDREIPPTSLLREFVGGSSFRY"
     gene            complement(7348..8649)
                     /locus_tag="PKH68_03280"
     CDS             complement(7348..8649)
                     /locus_tag="PKH68_03280"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX11992.1"
                     /translation="MNKFNFIYVKKYSFHVFVALSILVVIQPSVAGVKNYLQKPDSLF
                     NSYKGVVTDINTREKLPYASLTVESTNTSTVTNADGEFLLKVPKTDTDRMLVVTFMGY
                     GSQRISLTSMLPEGNKIELKSLPIYLPELKVISKDPEAVVQKMLDNRYANYSNKNVIM
                     NAFYRETIRKRNTNVSLSEALVEVYKQSYSNNMNDMAALYKSRKSTDYSKLDTLVYKL
                     MGGPFNCLYLDVMKYPEFIFTNSIFANYSFKFLQTDRIDDRLIYVIEFEQLKNVMEPL
                     FYGKLYIDAQSFALARADFDMNLTDNEEATKLFIRKKPYNAIVNTTKAHYQIEYRLRD
                     NKWYYAYSRVDLNMKINWKKKIFNTYYNSTIEMAATDWFDDVNKKEFKMKDRINPNVV
                     IQDAITGFSEPEFWGEYNIIEPDKSIENAINKIQKRLKKQE"
     gene            complement(8719..9444)
                     /gene="recO"
                     /locus_tag="PKH68_03285"
     CDS             complement(8719..9444)
                     /gene="recO"
                     /locus_tag="PKH68_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNX11993.1"
                     /translation="MQVKTTGIILHQIRYTDTSNIVSIYTRKFGRMSYFVRGASKKKS
                     ACRPALLQPLSVVEIEAVHSPKRTIHTLREMRVALPFYEIPFDQVKIALALFIAEILH
                     KTLKHSENDEELYHFIENSIRELDACKDGLGNFHLVFMTQLSHYLGFAPNMDNPDQAE
                     YFDLMNGIFIHQPPTHIHFLKPETTSALKKLTTLDYQNLNTMKLSRSERSELLDKLIE
                     YYQLHLPDFFPVASLDILHKLWE"
     gene            9660..12329
                     /locus_tag="PKH68_03290"
     CDS             9660..12329
                     /locus_tag="PKH68_03290"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNX11994.1"
                     /translation="MELASKYNPSEVEDKWYQYWLDNNFFHSEPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLVRRARMLGKNACWVPGTDHASIATEAKVVNRLAAQGISK
                     ADLSRDEFLKHAWEWTHEHGGIILEQLKKLGASCDWERTAFTMDKVRSESVLKVFIDL
                     YNKGLIYRGVRMVNWDPKALTALSDEEVIHKEVNGKLYYLRYRIDAPQPPEGGVGDPQ
                     YAVVATTRPETILGDTAMCINPNDEKNKWLKGKRVLVPLINRSIPVIEDEYVDMEFGT
                     GCLKVTPSHDVNDYVLGEKYNLPSIDIFNPDGTLNEHGAMYAGMDRFDVRKQIEKDLE
                     EAGLMEKIEAYTNKVGYSERTDVPIEPKLSMQWFLKMSELAKPALDNVENDTIKFHPE
                     KYKNTYRYWMENLKDWCISRQLWWGHRIPVYYAAPVNSFEGETSQQVIIVAENEEKAR
                     EIADNNPSLTNGYTLRQDEDALDTWFSSWLWPISVFDGINNPNNTDINYYYPTSDLVT
                     GPDIIFFWVARMIMSGYEYRQQPCFNNVYFTGIVRDKQGRKMSKQLGNSPDPIELMEK
                     FGADGVRMGMLLTAPAGNDLLYDDSLCEQGRNFNNKIWNAFRLVKGWEVADVEQSESA
                     KIAIEWFDSQLSKTMLEVDDLFTKYRLSEALMAVYKLFWDEFSSWYLEMVKPAYQKPI
                     DRETYNATLGFFDSLLKMLHPFMPFITEELWQALAVRQPGESIMIQLMPKAKQVDEKL
                     IAGIEKVKEIIAGVRTVRLQKNIPNKEPLQLQIIGDHQADFDAVIAKLCNLENIEKLT
                     EKSANAVSFLIGTTEYALPMGSLINVEEEIRKMEDEIVYLEGFLQSVIKKLSNERFVQ
                     NAKPEIVENERRKMADAETKIASLKESILSLSK"
     gene            complement(12405..12647)
                     /locus_tag="PKH68_03295"
     CDS             complement(12405..12647)
                     /locus_tag="PKH68_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HNX11995.1"
                     /translation="MLKLLFGIILFFLLLLLLAGAAGIGLLRTLFGGIKSPPRQPNET
                     NRPYSNPDKDKIFGDKEGEYVDYEEIKEDKTKNDVN"
     gene            complement(12819..13766)
                     /gene="metF"
                     /locus_tag="PKH68_03300"
     CDS             complement(12819..13766)
                     /gene="metF"
                     /locus_tag="PKH68_03300"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNX11996.1"
                     /translation="MSVVERIKSAQNTAFSFELLPPLKGNGIDGVYRTIDALREFDPK
                     YINITTHRSEVVFRENAQGLFEQIAERHRPGTVAVAAAIKNKYNIPVVPHIICSGFTR
                     EETEYVLIDLKFLDINDLLLLRGDKAKSEKGFTPTGHAHATDLQKQVNRFNEGYFLDG
                     SKMKIIDAPFSYGMACYPEKHDEAPNMESDLFWAKQKVDAGAEYLVTQMFFDNQKYYD
                     FVAKCRETGIDVPIIPGIKPVTFLNQLTLLPKIFHTDIPEDFARELRKCKTDEEASEV
                     GVEWCTMQAKDLVAHRVQSIHFYSLMATQSVRRVAAQVY"
     gene            13999..14469
                     /locus_tag="PKH68_03305"
     CDS             13999..14469
                     /locus_tag="PKH68_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HNX11997.1"
                     /translation="MKNKRERLGIISEILRMQVIGSQEELLDILRAKGYDVTQATLSR
                     DLKVLKVAKTPLSNGAYKYVLPPQIKPISDELNAHNFTSHGAVLNLEFSGQLAVLKTK
                     PGYASAIAWDIDNRSNDEVLGTIAGDDTILLIPREGVTRADITKMINMIFQEKS"
     gene            14489..15163
                     /locus_tag="PKH68_03310"
     CDS             14489..15163
                     /locus_tag="PKH68_03310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX11998.1"
                     /translation="MNKKHTENKDITVQVADISHLTYVQTILTTIEEAAKIRGTGIAK
                     RTPEYIQQKIKESKAIIALKGDEFAGFCYIETWGNKKFVANSGLIVVEKFRGHGLAKL
                     IKQKAFQLSRERYPDAKIFGLTTGLAVMKINSELGYKPVTFSELTNDEAFWKGCQSCV
                     NYDILQRTGRKHCLCTGMLYDPAWQENNGTKKTPRKNKMWKLIQVMGEWMSPRILNQK
                     QQKEKE"
     gene            15175..16374
                     /gene="argG"
                     /locus_tag="PKH68_03315"
     CDS             15175..16374
                     /gene="argG"
                     /locus_tag="PKH68_03315"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNX11999.1"
                     /translation="MKEKVVLAFSGGLDTSFCAKYLAEEKGYEVYTAVANTGGFSDVE
                     LKIIEEKAYRLGAVKHVAIDVTREYYEKSIKYMVFGNVLRNGTYPISVSSERIFQAIA
                     IINYAKEIGAAYVAHGSTGAGNDQVRFDLTFQILAPEIKILTPTRDMTLTREYEISYL
                     KEHGYTADFKKMEYSINKGLWGTSIGGKETLKSEQTLPESAYPSQVVKTGEETISIGF
                     EKGEIVSVNGENSEDKIHLINKLEDIANKYGIGRDMHIGDTIIGIKGRVGFEAAAPML
                     IINAHKMLEKHTLTKWQQYWKDQLGNWYGMFLHEAQYLEPVMRDIEQFLESSQQNVTG
                     TVTVKLRPYSYTLVGVESDFDLMKTDFGEYGEVNKAWTADDVKGFTKILGNQMKIFYN
                     VQKRNEK"
     gene            complement(16926..>18210)
                     /locus_tag="PKH68_03320"
     CDS             complement(16926..>18210)
                     /locus_tag="PKH68_03320"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNX12000.1"
                     /translation="APFWLHSDRYGKISSSPMSALIFTGIEKQYTNPEKRFDYGFKAG
                     AIVRTDYHKNEIFLNEIYAKARYAVFNIMAGSREQIFGNQDSTLSSGGLLFSKNARPM
                     PEISVGIDNFTPVPFTFGLLELKGSIRQGWFTDNIYTRGIMLHHKFGYVRLGGKLPVH
                     VQYGLEHVAQWGGTDPVTGPLPHGLKNFGRIFLAKSGGEDASLSDQINVLGNHLISQG
                     FKVEADVAGIRVNGYWQNISEDRPIKFIGSNEVNKPDGLWGISVKSNKFPFIKGVLYE
                     LLNTTDQSGPYHDKDGLIYGGTDGYFGGAYPVGWSYYSRTIGTPFITSPLYNINGALS
                     TTNNRVRLHHFGIEGSIEGFEYRALASFSRNYGTYGSFVNIPNKSFLLEVNKHISLLS
                     GFDLSCAAGGDVGKFYGNSVGIMVSIRKTGFLFGY"
BASE COUNT         5274 a         3705 c         3861 g         5370 t
ORIGIN      
        1 aacaacttct gatgcggttt cagagctaat tttgcagcaa tggatttaca ttcatcaggt
       61 tgagtttctt ccggacattt tgtaatcacg accatatcag cgcgtttgct gttcgtctta
      121 aattctctca acgtaccgta aggaagcata taatcatctg tgtacagatt tgcataatca
      181 gtcagcagta tggataatcc cggcttcaca taccggtgtt gaaaaccgtc atcaagtata
      241 atcacctgaa catcaggatg aattttcagc agattttgga tgccacgaac ccgcttttcg
      301 tccacagcta cagtcacctg cgggaattta ccgaaaatct gaaacggttc atcgccaata
      361 ctttgcgcag tgctatttgt atctgcaagt acaaagttgg tagtttttcg cctgtagccg
      421 cggctcagaa ccgccgttct gaatttttcg ctcaactgtc ggataagaaa ttctgtatgc
      481 ggagttttcc ccgtgccacc cactgccaga tttccgatgg agataacagg catatcaaac
      541 gaatgctgtt tcaaaattcc ccaatcaaag aagcagttgc ggattgtggt caagctgcca
      601 taaatcagag aaagaatata aaaaataata tttttgccgg gctttaacaa tgatttcttt
      661 ttatacaaag atatgaaatg ttttgtaaaa aggggaatga atagggttca atttgaaatt
      721 cggagttcag tataaaaaaa gcgtttccgt aaaccgaaaa cgctttgggt tattttactt
      781 atcaacaaat taatttgtgg tgatttcaaa tggcagaacc gcctgtatgc cgttgaattt
      841 ttccaatcgt ttgatttgtt tcaacacttc gtatttttca cctaactggc cttccatgat
      901 gcgggtttcg attgtagaat taaagtcttt cattattcgg gtcataaaac tttccttttc
      961 aggatactct ctgatcatat aattggagat ttttgctttt tttgctgcca ttgcaatagc
     1021 atcgttcaat ccaccgattt tatccaccaa accaatcttt tttgcatctt cgccggtcca
     1081 aacacgtcct tcggctatgg cttttatttg atccggagtt ttcttacgac cgtcggcaca
     1141 gcgttttaca aatatttcat aaccccggtt tacgtaattt tgcatgatat tccgctcgtc
     1201 gggggtgaat gctctgttga agtcaatagc gtcgctcaat ttattggttt tcacaccgtc
     1261 gtaagtcaat cctaatttat cgttcagtcc tttgatattc ggaattacac cgaaaatacc
     1321 gatagaaccg gttaaggtat tgggttgagc cacaatgctg tcggccatgc acgaaatgta
     1381 atatcctccg gatgcggcat agtcgcccat cgatacaatg accggttttt tagcttttag
     1441 ttcgctcagc gcatgccaga tttgttcaga tccataagca cttcctccgc ccgaattaac
     1501 ccggaatacc acagctttca ccgatttttc atctccaact ttccggatgg tttcaaccag
     1561 gtctttcgat atgatgcctt ctgtgtcgcc catatcaatg cctccaacgg cgtaaatgac
     1621 tgcaattttc tctttctcca tttttactga tgctggtacg cgggctaaat ctgattggtt
     1681 gatggtctcc aacttgtcat ctttatctac tttcatcatt tttttcaata cattttccat
     1741 accatcgata taggtcagcg aatcgaccat gcggtattgg agaagttttt ctgcaggcgc
     1801 gaatgtcatc atttcgtcag cataccgatt caatgtatct ttatgtattt ttcgcccatc
     1861 tgatatttcg gtcagcagat tattccagat tgaaccaatg aaggctgtaa cctgttcttt
     1921 gttggcgtca ctcattttgg tattcacata cggttctacg gccgatttga atgtgccgac
     1981 ttttaccacc tgagcttcaa ctccaagttt gtccagcgtg tttttcagaa acatggtgtt
     2041 ggctgcaatt ccctgaaatt caatcattcc ctgcggattg aggtagattt tatcagcggc
     2101 agagcataaa tagtacattt tttgagaata ggtatcggca tacgccacca gaaattttcc
     2161 cgatttctta aaatcgagaa gagcatccct gatttccttt acagaggcat atccgcccat
     2221 cagtgtaccg cctttcagat agataccttt tatgttttta tcggttttag ctttttttat
     2281 gtttgccagc acctcgtcca gaccaaggtt tttcacctca ttcctgccaa tggcatccga
     2341 gaatattccg gagaatggat cgtcctgaga gcgttcaatc agctgtccct ccagatcaag
     2401 ctggtacaca gagttggggt cgacctttac ggttttttca ctggtgcttg caatagcccc
     2461 gataaagcca aataataaca aaaaaccaag cagggaagtg ataaatatcc ccacaattgt
     2521 agccagggta aatttcagaa attgtttcat aagttttgat ttttcaaatg attatgaatg
     2581 caaaggaaca aaataaattg aacacaaaaa tcatttattt aaataaaaat gtgaaaaact
     2641 gtaatttgtt ttcaggcgtg agcgagtaat gcgttcttca ggttttgttt gtcgagacca
     2701 ccgggatgtt tccaaacaac agtaccgttt ttaaaaatca taagggtagg tacagcctgt
     2761 atctggtatc gggctgcaag agccggattt ttatccacat caattttcaa aacacgagct
     2821 ttgccctgca cttccttgcc tatttgttct acaataggag ccattgtttt acacggaccg
     2881 caccaggtgg catagaagtc aatcagcacc ggtttatcgg catttattat ttcgttgaag
     2941 gtttccataa attactacta tgaattattg ataagattga atgagtttaa aaaataaaaa
     3001 ggaaggcgaa ttcactcgcc ttcctgaaag ttggtttttt acttaatttc tatcatgcgt
     3061 gccggtttcg gtttagcttc ctcttttttg gggattgtaa ccagcaatat tcctttatca
     3121 tacgctgcgc tcacattatc gccattggca gtggcaggca gtgtaaatga tcgggtgaaa
     3181 gcctgatagc tgaattcgcg tttggtgtag tgttcctttt catttttggt ctcattttct
     3241 tcctgtttct ccgaagaaat ggtcatcagg tcgttgtgaa cctcaatttt gaaatctttt
     3301 ttgtccattc ccggtgctgc tacttctacc ataaaggcat catcattttc cttgatgttg
     3361 accgatggca atgtggtatt ggtttgtgaa aaatttctgt ttgaccagtc taataaatca
     3421 ttgtcgaaaa agcggtcaaa ccacggtgtt tgtgaaaatc ttacgagtga cataatttaa
     3481 tcctccatat ttttaagtta aacattaatt gttttgttgt ttgaacctga aaatacaaac
     3541 ttaatgccaa taggaaatgt gtgccataat ggcaggttga ttggattgag aggattttta
     3601 catgaattaa aaattcggat agcaggcgtg agtgtgattg gtcaaatgtc tgtatatcag
     3661 caaagagaac cttattcaat aaaagaaatt cggccagaaa acaggagtga caaaatggca
     3721 gttttgctca aactgtaatg aagcataagt agtagaaaac gatgtggatt agaaattaat
     3781 caacatcaca gcggcatttc ttagtttgga aataaatgcg ggtaaaataa tacgagaatg
     3841 taaacatggc tccataacca acatcgttgc ttttcgacca gctggtagta aacccttcca
     3901 cataatccga aaaataatac tcataactca gatccagacc aacggcaaaa tttctggaga
     3961 gacggtattg atacccgata cctgtaagca ataatggtgt ggtaacgggc gttgtattta
     4021 caaagtggtc tttatccgga tctccaacta ctgtggaagt tgcataagca acacctccac
     4081 ccccgaacac gtatacattg tggcgggcct cccagtctgt tacattacca agaatgtgat
     4141 attcaagccg ggcgcctcct ctgtaaattt gattagaaaa tttatattta cctcctcctc
     4201 ctctggtgtc gttttttgtt cccttatcat cgtctgcgta cgtggtaaat tctccgaata
     4261 attttatccc aaaattggca ggcaacagat atctgaaacc gaaattaact gttggtgata
     4321 aatcacctgg ttgtttttcc catagtttcc ctccgatatc aaacgaaggg agagaaaaac
     4381 cacttgtaat gataagtgat ttgtctccta tcgaaaattt atagtcagat tcaccactct
     4441 gcccaaaagc agatgatatc cagattgtta gaaggatgta aagtattatt tttcgcatca
     4501 gataaatttg tcatcaaaat tattattcaa ggatattacc ggctaacaaa cttattaaaa
     4561 aaaatcggat atgacccaac aattatatga ttaatttggc tgctgaaaag ccaattttta
     4621 aatctttatt tcgggtttac cctgattttt tttaacgaac agattcttgc atctaattta
     4681 tcttctttgt ctccgatccc atctgttatt gaattcactc attcttctac tgaacgggaa
     4741 atagtaagct gccgtcagtt tcaggtcaaa agcctcatcc ctgtgtttgt tggctcgaat
     4801 tacgggatta tagccgtcca ccctgtctga atgaaaatag ttttgtttgg cttccactcc
     4861 gaaactaaac tgattggaga agcggtactg atatccaata cccaaaaaat gggttaaagg
     4921 attttttgtc tcttccggac cattagcatt gttttcggga ttaattagtg tggcatccac
     4981 ttttgttgtt ttcagtccgg ctaacagata taccgaatgt ggtatcgggt ctttggccag
     5041 aggattgcca aagagtacaa cctccaactg gccgcccaaa cccgtaacct ttgactgaaa
     5101 ttcaagtttt ctgacagctc cgtaggtatt gtattccact ttatcctttc cccggtaacc
     5161 ggagtagtcg ccaaaaactc tcaaagcgaa gttgtatttg aatgtatagc gggctccgag
     5221 tgaaagtccg tagcccatgt cgttttgaaa caggcttttg aaattatcac ctcctatgtc
     5281 gcccgacagt ttattgggtc cgacaccgcc aaacagcgaa aattcgccta acggcatttg
     5341 ggtaagcaat ccttttccct gagggaaaac ggatgcgata aacaacagaa aaattgaaac
     5401 aattattagc tttttcataa gcggttaaag tgtaggggtt caaatcatag ttctgtttaa
     5461 ctcatattga agtatttaat ttttttcatt taatacggat gcatttcgta ttcaaattca
     5521 tacattttca ggttattcaa attaagctgt cattaatatc ggaagctgct cccacccaca
     5581 aattcgcgca ggagcgaagt gggaggaatt tcacgatcgg gaatggcagt gaaataacgc
     5641 aggaatttca tcaggcggtg tgtttcggtg tattcatcac aatatttggg tacctgttcc
     5701 agtatcggtt ccacgtgtct tttcagcact gccagttcaa atatcagggc ggaattaaac
     5761 attacatcgg cattttcctg gtagggaaaa atccattttt cttcgccacg tcgcacactc
     5821 ggccagcgcg atatggtatc gcgtgctgag tagccgcggt aacgataatc ccgtataatg
     5881 cgccgtagta gccgcgtgtc ggcagtgggt atccagttgt gctcatcgat ggaaatggta
     5941 gtaagggctg atacatagat tttgaaaatg gcatcctgtg gaatggattg aataagttcc
     6001 ggattgagtg cgtgtattcc ttccatgatc aggatggtgt cttcctccag tctcaatttt
     6061 tcccctttga aatgccgttt tccatcctca aaactatagg atgggagagc gatttcttca
     6121 ccattcataa gttgactgag ttgttgattg aaaaatggaa ggtcgagtgc atgaagtgct
     6181 tcaaaatccc agtcgccgtt ttcgtcacgc ggggtgtgtt cgcgatccac aaagtaattg
     6241 tcgagtgaaa ttgtttttgg ctttaatccg ctcaccatca gctggatgga aagtcgcttg
     6301 ctgaaggtgg ttttacccga cgaagagggt ccggatatca tcactacctt tactgttggt
     6361 cgtgcctgta tcatatcggc aaccgatgcc acttttttct catgaagagc ttcggctatt
     6421 tttatcaggt tgaatgcctg attgttctgg caggcacggt tgaattcgcc caccgatttc
     6481 agatccatga tgcggttcca gcccaggtat tccttgaaaa tttcgaacat tttaggctgt
     6541 agaaccatat cctccaactg gtcgggatta tcccgatcgg gaacctgcaa cagcatgctg
     6601 tcgtagaaag gtatcagatc aaagatattc agatatccgg ttgagggaag caggatgctg
     6661 gtgtagtaat cgatgaagtt tccaattcgg aaatagcgaa gatacgggtc gcccagactt
     6721 tcgaaaagtg tgtctttgga tgtgttattc gtgccgtttc tgaacagttt tttcacatgt
     6781 tccgcttgtt gttcctcgca gataatgggt tcattccggc tgataatttc cctcattttt
     6841 gcctttatcc ggttcacaat gtcggttgta agtggctcat tgaggtgttc tatccggcag
     6901 taatatcctt tggaaatggg atgctctatg cggatttcgg tatgcggata caattccgat
     6961 accgctttcg aaagtaccat gctcagactt cgtacataca cacgcatacc cgatgatgtg
     7021 ctggtatcaa taaactcaat atcttttggc ttgtaaatca gaaaattaag atcctctacc
     7081 ttgtaattga cacgggcggc aactacctga tatttaagat tgatggaaag tgaattatat
     7141 atctcgagca gggaagtccc taatggaaaa tcatgataat tttgagtgtt tttacaataa
     7201 attgttacag ttttatccat aaaataaatg tttcaggcaa gattaatata aaagcattca
     7261 cttccgtttt cagttagaaa cagatattaa gaccaccaca aatatagttg atattttcga
     7321 atatcaatta tcgtgttcaa aataaattta ttcctgcttt ttcagtcttt tttggatttt
     7381 gttgatggca ttttctatcg atttgtcggg ttctatgatg ttgtattctc cccagaattc
     7441 cggttccgaa aatccggtaa tggcatcctg gattaccaca ttggggttaa tgcggtcttt
     7501 cattttgaat tcctttttat ttacatcgtc aaaccaatcg gtagcggcca tctcaatggt
     7561 ggaattgtaa taggtgttga atattttttt cttccagttg attttcatgt tcagatccac
     7621 ccggctgtat gcgtagtacc atttgttgtc tctcaaccgg tattcgattt gataatgggc
     7681 ctttgtcgtg tttacaatgg cattgtatgg ctttttacgg ataaaaagtt tggtagcttc
     7741 ttcgttgtcg gttaggttca tgtcaaaatc ggcacgtgcc agcgcaaacg attgagcgtc
     7801 aatgtacaat ttcccataaa acaaaggttc catcacattt ttcagttgtt cgaattcaat
     7861 cacgtagatc agtcggtcat caatccggtc tgtttgcaga aatttgaaac tgtaattagc
     7921 gaaaatggaa ttggtaaaaa tgaattcggg atacttcatt acatccagat aaaggcagtt
     7981 gaatggtcca cccatcagtt tgtataccag tgtgtcgagt ttgctgtagt cggtgctttt
     8041 tctcgatttg tacagtgcag ccatatcgtt catattgttg ctgtacgact gcttgtacac
     8101 ttccactaag gcttccgata gtgaaacatt tgtatttcgc tttctgattg tttcgcgata
     8161 gaaagcgttc attatcacat ttttattgct gtagttggcg tatcggttgt ccagcatttt
     8221 ctgtactacc gcctcaggat ctttcgaaat tactttcagt tccggcagat aaatgggcaa
     8281 tgattttagc tcaattttat tcccttcggg cagcatggaa gtgagcgaaa ttctctgaga
     8341 gccatagccc ataaatgtga ccacgagcat tctgtcggta tccgttttgg gtactttgag
     8401 cagaaactca ccatcggcat tggttacggt cgaggtattg gtactttcta ccgtcagcga
     8461 tgcgtaaggc agtttttcac gcgtattgat atcggttaca acccctttgt aagaattgaa
     8521 aagcgaatcg ggcttttgca aataattttt tacacctgcc accgaaggct ggatcactac
     8581 aagaatactc agagcaacga aaacgtgaaa agagtatttc ttaacgtaga taaaattgaa
     8641 tttattcatg ataataatgt gttttttctg ttactaaaac aaccactgtc atttaatgtt
     8701 gtatgtcgat aaaaaatatt actcccaaag tttgtgcaga atatccaacg aagcaaccgg
     8761 aaaaaaatct ggcagatgca gctgataata ctctatcagt ttgtctaata attcagaccg
     8821 ctcgctccgg ctgagtttca ttgtattcag gttttggtaa tccagcgtcg tcagtttttt
     8881 cagtgcactg gttgtctccg gcttcaaaaa atgaatatga gttggtggct gatgaataaa
     8941 tataccattc attaagtcga aatactcagc ctgatccgga ttgtccatat tgggtgcaaa
     9001 gcccagatag tgcgataatt gtgtcataaa taccaaatga aaatttccca aaccgtcctt
     9061 gcaggcgtcc agctcacgga tggagttttc aataaaatgg tacaattctt catcgttctc
     9121 tgaatgcttc agggttttat gcagaatttc ggcaataaat aatgccaggg caatttttac
     9181 ctgatcgaat ggaatttcgt aaaaaggcag tgccacgcgc atttcccgaa gggtgtgaat
     9241 agttcgcttc gggctgtgaa cggcttcaat ctccaccacc gaaagaggct gaagaagtgc
     9301 cggccggcag gcagacttct tcttgctggc tccccggaca aagtatgaca ttcgcccgaa
     9361 ttttcgggtg taaatgctta caatattcga tgtatcggta tatcggatct gatgcaggat
     9421 gatacccgtg gttttaactt gcattttaca aaaataagaa taattctgct gaaatattct
     9481 taacgctgcg tttttgtttg gtttatatga gctttttggt tcaaaataat aataaaaatg
     9541 cttttttagt tatctttgca ctttgttttt tcaacaaaag aaataaaaga tgagattgaa
     9601 attcagtaag tttgagctcc gatcctaagt tttgaaaatc gtaaattgta ataaataata
     9661 tggaacttgc aagcaaatat aatccttcgg aagtggagga taaatggtat cagtattggt
     9721 tggacaacaa ttttttccac tccgaacccg atgaacgtga accttatacc atcgtaattc
     9781 caccgcccaa tgtaaccggt gtgctgcaca tgggacacat gctgaacaat accatacagg
     9841 acgtactcgt tcgccgcgca cgcatgctgg gcaaaaatgc ctgctgggtg ccgggtaccg
     9901 atcacgcgtc aatagccacg gaagcaaaag ttgtaaaccg gctggcagca cagggtatca
     9961 gtaaagcaga tctcagccgt gatgaatttc tgaaacacgc ctgggaatgg acacacgagc
    10021 atggtggaat catcctggaa cagctgaaaa aactcggtgc ctcctgcgac tgggagcgca
    10081 cggctttcac gatggacaag gtgcgttccg aaagtgtatt gaaagttttc atagatcttt
    10141 acaataaagg gctgatttac cgcggggtac gcatggtcaa ctgggacccc aaagcactca
    10201 ccgctctgag cgacgaagag gtaattcata aggaagtgaa cggaaaattg tattacctcc
    10261 gatacaggat agatgccccc caaccccctg aagggggagt tggagatcca caatatgctg
    10321 tggtagcaac aacaagacct gaaactattc ttggtgatac agcaatgtgt atcaacccaa
    10381 atgatgaaaa aaataaatgg ttaaaaggaa agcgtgtttt ggttccatta attaaccggt
    10441 caatacccgt cattgaggac gaatatgtgg atatggaatt cggtacgggt tgcctgaaag
    10501 tgacaccatc tcacgatgta aacgactacg tgttgggaga aaaatacaac ctgccttcca
    10561 tcgatatttt caatcccgat ggcacactca acgaacacgg tgccatgtat gccggaatgg
    10621 accgctttga tgtgcgcaaa cagatagaaa aagacctgga agaggctggg ctgatggaaa
    10681 aaatcgaagc atacactaat aaagtgggct actcggagcg taccgatgtg cctatcgaac
    10741 ccaaactgtc gatgcaatgg tttctcaaga tgagcgaact ggcaaaacct gcgttggata
    10801 acgtggaaaa tgacactatt aaatttcatc ccgagaaata caaaaacacc tatcgctact
    10861 ggatggaaaa cctgaaggac tggtgtatca gccggcaact gtggtgggga caccgtattc
    10921 cggtgtatta tgcggcccct gtaaattcct tcgaagggga gacttcccaa caagtaatta
    10981 ttgttgcgga gaatgaagaa aaagcacggg aaattgcaga taataatccc tcacttacca
    11041 atggttatac gctccgacag gacgaagatg cgctggatac ctggttctca tcgtggttgt
    11101 ggccaatttc tgtttttgat ggaataaata atccaaataa tacggatatt aattactatt
    11161 atcccacaag cgacctggta acaggacccg atatcatttt tttctgggtg gctcgtatga
    11221 ttatgtcggg ctatgaatac cgccagcaac cctgtttcaa caatgtgtat ttcacaggaa
    11281 ttgtacgcga taaacagggt cggaaaatgt caaaacaact tggtaactct cccgatccga
    11341 ttgaactgat ggagaaattc ggtgctgatg gagttcgcat gggtatgtta ctgacagctc
    11401 ctgccggaaa cgatttgctt tatgacgaca gcctgtgtga gcagggacgc aacttcaata
    11461 ataaaatatg gaatgcattc cgtttggtta aaggttggga agtggcagat gtggaacaat
    11521 ctgagtcagc aaaaattgct attgaatggt ttgattctca actttcaaaa acaatgcttg
    11581 aggtagatga tttatttaca aaatatcgtc tttcggaagc tttgatggct gtttacaaat
    11641 tgttctggga tgaattttct tcctggtatc tcgaaatggt aaaaccggcc tatcaaaaac
    11701 cgattgaccg tgaaacctac aacgctacgc tcggattttt cgatagtttg ctcaaaatgc
    11761 tgcatccgtt catgccattt atcaccgaag aactctggca ggcactggca gttcgccaac
    11821 cgggagaaag tatcatgata caactgatgc caaaggcaaa acaggtggat gaaaagctga
    11881 ttgccggtat cgaaaaagta aaagaaatta tcgccggtgt acgtaccgtc cgtttgcaaa
    11941 aaaatattcc taacaaagag ccacttcaat tgcaaattat cggtgatcac caggctgatt
    12001 ttgacgcagt aatcgccaaa ctttgtaatt tggaaaatat cgaaaaacta accgagaaat
    12061 cagccaatgc tgtatcattc ctcattggaa ctactgaata tgccttaccg atgggtagtt
    12121 tgataaatgt ggaggaggaa atccggaaaa tggaagatga aatcgtttat ctggaaggat
    12181 tcctgcagtc ggttataaaa aaactgagca acgaacgctt tgtacaaaat gccaaacccg
    12241 aaatagtgga aaacgaacgc aggaaaatgg ccgatgccga gactaaaatt gcttcgctga
    12301 aagaaagtat tttatcattg agcaaataat catatagctt acatggataa aaaaggcacg
    12361 aagaggtatt ctttcgtgcc ttttatttta tgaaagtgag gaatttaatt cacatcattc
    12421 ttcgttttat cctcttttat ttcttcatag tccacatatt cgccttcttt atcaccgaat
    12481 attttatctt tatccgggtt ggaataaggc cggtttgttt cgttgggttg tcggggcggt
    12541 gacttgatgc ctccaaacag ggttcgcagt agcccaatgc cggcagctcc tgccagtaac
    12601 agcagcagca aaaaaaagag tataattcca aataatagct ttaacataat ataaaaataa
    12661 ctgtttttta atgaccggaa cggtaaattc agtatgcaga tttaggtttt acaatctaaa
    12721 atttacaatt tgtgctttta agaaatgctt tacattgaat tattttgaga ttttttggct
    12781 catttagtcc ccctttaggg ggatttaggg ggcttctatt aatatacctg tgctgccacc
    12841 cgtctcacac tttgtgtggc catcagactg taaaaatgaa tactttgcac acggtgcgcc
    12901 accaaatctt ttgcctgcat tgtacaccat tccacaccca cctcagaggc ttcttcatcc
    12961 gttttacatt tacgcagttc gcgggcaaaa tcctcgggta tatcggtgtg gaaaattttt
    13021 ggcagtaacg tcagctgatt caaaaaggta accggtttaa tgcccggaat aatcggcaca
    13081 tcaattcctg tttcgcgaca tttggctaca aaatcataat atttctgatt atcgaaaaac
    13141 atttgagtaa cgaggtattc ggctcctgcg tccacttttt gtttcgccca gaacaaatca
    13201 gattccatat tcggcgcttc atcgtgtttt tcggggtagc aggccatgcc atacgaaaaa
    13261 ggtgcgtcaa ttattttcat cttcgagcca tccagaaaat atccttcatt gaaccggttg
    13321 acctgcttct gtagatcggt agcatgggcg tgacctgtgg gggtaaaacc tttttcgctt
    13381 ttcgctttgt caccgcggag aagtaacagg tcattgatgt ccagaaattt aagatctatc
    13441 agaacgtatt cggtttcttc gcgggtaaaa ccgctgcata tgatatgtgg tacaaccgga
    13501 atgttgtatt tgtttttgat tgccgctgcc acagccactg taccgggcct gtgtctttcg
    13561 gctatttgct caaataagcc ctgcgcattt tccctgaaaa caacttcaga gcggtgtgtg
    13621 gttatgttaa tgtacttcgg gtcaaattcc cgcagggcat caatcgtgcg gtacaccccg
    13681 tcaatgccgt ttcctttcag cggaggaagc agctcaaacg aaaaggcagt attctgtgcg
    13741 gatttaatcc tttcgacaac actcattcaa taataatttt agactgcaaa aatacaaaag
    13801 aaatttcgaa aatagcttta ttgacaagga cggtataacg aaaaattcat ttttatgttg
    13861 aaaattagct tttttccttt ctttaattgt ataaaattac atgaaaaaca ataaatatcg
    13921 aaaataaact tcatttattt gcataaatct gcataaagtt gtatttttgc accggatatc
    13981 taataaatat acgaaaacat gaagaataaa agagaacgtt tagggataat atccgaaatt
    14041 cttcggatgc aagtaattgg cagccaggaa gaactgctcg atattttacg cgccaaaggt
    14101 tatgatgtta ctcaggcaac cttatcacgc gatttgaaag tgctgaaggt agccaaaaca
    14161 ccactctcca acggagccta caaatatgta ctgcctccac agataaaacc catctcagac
    14221 gaactgaatg ctcataattt cacgtcgcac ggtgcggtac tgaacctcga gttttcgggc
    14281 cagcttgctg tgttaaaaac aaaacccggg tacgccagtg ccattgcgtg ggatattgac
    14341 aatcgttcga atgatgaagt actcggaaca atagccggag atgatacgat tttattaata
    14401 ccccgtgaag gtgtaacacg cgctgacatt accaaaatga ttaacatgat ttttcaggaa
    14461 aaaagttaga aggagtttat aaactaaaat gaataaaaaa catacagaaa acaaggatat
    14521 aactgtgcag gtggctgata ttagccactt gacttatgtt cagaccatac taacaaccat
    14581 cgaagaagct gcaaaaattc gtggcacagg tatagccaaa cgtacccccg aatatattca
    14641 gcaaaaaata aaggaaagca aggccatcat cgcacttaag ggtgatgaat ttgccggctt
    14701 ctgctatatc gaaacctggg gaaacaaaaa gtttgtggct aactcaggtt tgattgttgt
    14761 ggaaaaattc agggggcacg ggctggccaa actgatcaag caaaaagcat ttcagctttc
    14821 gcgcgaaagg tatcccgatg ctaaaatctt tggattaact accggcctgg ctgtgatgaa
    14881 aatcaattcg gaactgggct acaaaccggt aacattctca gaactgacca acgatgaggc
    14941 attctggaaa gggtgccaga gttgtgtaaa ttatgatatt ttgcagcgaa caggccgcaa
    15001 acactgcctt tgtaccggta tgctttatga tccggcctgg caggaaaata atggtacaaa
    15061 gaaaactcca cgaaaaaata aaatgtggaa actgatacag gtgatgggtg aatggatgtc
    15121 gccccggata ttgaaccaga aacaacagaa agaaaaagaa taaatcacga aacgatgaaa
    15181 gaaaaagtag ttttagcatt cagcggtgga ttggacacct ccttttgtgc caaatattta
    15241 gccgaagaaa aaggatatga ggtttatacg gcagtagcca ataccggtgg atttagcgat
    15301 gttgaattga aaataatcga agaaaaggca taccggctcg gtgcagtaaa acatgtggcc
    15361 attgatgtga cccgcgagta ttacgaaaaa agcataaaat acatggtttt cggcaatgtg
    15421 ctccgcaacg gtacctatcc catttcggtg agttccgagc gtatttttca ggccattgct
    15481 atcatcaatt atgccaagga aattggtgct gcgtatgtgg ctcacggcag taccggggcg
    15541 ggtaacgatc aggtgcgttt cgacctcact ttccagattt tagctcctga aattaagata
    15601 cttactccta cacgcgatat gactttgaca cgtgagtatg aaatcagtta tctgaaagaa
    15661 cacggttaca cggctgattt caaaaaaatg gaatattcca tcaacaaggg gctttgggga
    15721 acaagtatcg gtggtaagga aacgctcaaa tctgaacaaa cgctgcccga aagcgcttac
    15781 ccgagccagg tggtgaaaac gggtgaagaa accattagta ttggttttga aaaaggggaa
    15841 atcgtttcgg taaacggaga aaattcggaa gataaaatcc accttatcaa caagctggag
    15901 gatattgcca ataaatacgg tatagggcgc gatatgcaca tcggcgatac cattatcggc
    15961 atcaagggca gggtagggtt tgaagcggca gctcccatgc ttatcatcaa tgctcacaaa
    16021 atgctcgaaa aacatacgct cactaaatgg caacaatact ggaaagacca gctgggcaac
    16081 tggtacggca tgtttctgca tgaagcgcag tatctggaac cggtgatgcg cgacatcgaa
    16141 cagtttcttg aaagttcaca gcagaatgta accggaacgg taacggtgaa gctccggccg
    16201 tacagctata cactggtggg tgtggaaagt gattttgacc tgatgaaaac cgatttcggc
    16261 gaatatggtg aagtgaacaa agcctggaca gctgacgatg tgaaaggttt caccaaaata
    16321 ctgggcaatc agatgaagat attctacaat gtgcagaaaa gaaacgaaaa ataaaatgca
    16381 ataaacttac gtgagttcaa ggtaagatta ctcctttcag gtaatataaa cacaccccaa
    16441 tctgaaaatt tccagattgg ggtgcttttt tattgttcct tcattaaacc agaagagggt
    16501 tcaacttcgc ttcagattgc tggctgataa actaataaac cttagatatc tcatagataa
    16561 accatagata tcccatatat aacatatatc atatttgata tgggatatct atatgaagaa
    16621 tataatatat ataaatgata atcctgttta tacagactta atgatatggg aatgcatact
    16681 atataaacca cagatagtcc gtagaatttg cttaggaagc atggcaaaag gatggaatgc
    16741 ccgggaataa gtttctttga agtaactact ttttaggcta atcttattta aggagagaca
    16801 ttctggaatt cattttgcag aactcacttt aatctcaatc ggattgaaaa agtgaagtaa
    16861 aaaaaggaag tgacatgtgt aaaagttttc aggagattaa atcccgattt ttttttattg
    16921 cggggttagt aaccgaaaag aaatccggtt tttcggatgg aaaccattat gcccaccgaa
    16981 ttgccgtaaa atttacccac atctccaccc gcagcgcaac tcagatcaaa tcccgacagc
    17041 agtgaaatat gtttgttcac ctccagcaga aatgatttgt tgggaatatt cacgaatgag
    17101 ccataggtgc cgtaatttcg gctaaaggat gccagcgccc tgtattcaaa gccttcaatg
    17161 ctgccttcga taccgaaatg atgcagacga acccggttgt tggtggtgct cagcgctcca
    17221 tttatattat agagcggtga ggtgataaag ggagtgccga tggtgcggga gtagtaactc
    17281 cagccaaccg gataagcacc accgaaataa ccgtcggtac cgccgtaaat caatccgtct
    17341 ttatcgtgat agggtcccga ctggtcggtg gtgttcagca attcatacaa tacgcctttt
    17401 ataaagggaa atttattgct ttttaccgaa attccccata gtccgtcggg tttattaacc
    17461 tcgttggaac cgataaattt aatgggtcgg tcttccgaaa tattttgcca gtaaccattc
    17521 accctgattc cggccacatc ggcctctacc ttgaacccct gcgaaatcag gtgattaccc
    17581 agtacgttga tctgatcgct caggctggca tcctcgcctc ccgatttagc cagaaatatg
    17641 cggccgaaat ttttcagtcc atgaggcaga ggtcctgtaa ccggatcggt gcctccccac
    17701 tgtgccacat gctccagacc atactgcaca tgaaccggca gttttccacc caaccgaacg
    17761 tacccgaatt tgtgatgaag cataatgccc cgtgtataaa tattatcggt aaaccaaccc
    17821 tgccttatac tgccttttag ttcaagtaaa ccaaaggtaa atggcacggg agtgaaatta
    17881 tcaattccca ctgaaatttc aggcataggt cgtgcatttt tggagaagag caagccaccg
    17941 cttgacaggg tagaatcctg attgccgaaa atctgttccc tcgaacctgc cataatatta
    18001 aaaactgcgt atcgtgcttt ggcgtagatt tcattcagaa atatttcgtt tttatgatag
    18061 tcggttctga ctatagcccc ggctttgaaa ccataatcaa accttttttc cgggttggta
    18121 tattgttttt caattcctgt aaaaattaaa gcactcatcg gggaggagga tattttcccg
    18181 taccggtcgc tgtgaagcca gaatggagca
//
LOCUS       DAOPZK010000032        17985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3278_length_17985_cov_25.230731, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000032 DAOPZK010000000
VERSION     DAOPZK010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17985
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3278_length_17985_cov_25.230731"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2007
                     /locus_tag="PKH68_03325"
     CDS             <1..2007
                     /locus_tag="PKH68_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX12001.1"
                     /translation="FINFGQTRVDNYYWMKDKNNPKVIDYLKAENAYTDTVMASTKDL
                     QKKIYDEIIGRIKEDDQSYPTLSNGYYYYTRTEKGKQYRTYCRKNGSEKAPEEIIFDV
                     NKMAEGKPAFMFGGYDVSPDNQLAGYFYNETGSFADYVLKIKDLKTGKDMDFAVKGAS
                     SFAWANDNKTLFYSVIDPVTLRSNRVFRQVLGNPKAVLIYEEKDTKFSLGVTETKTRD
                     FIFINTGSTNTTEELFVPADKPAEAFKIFMPRVKDVDYSVFTHRDYFFIRYKDKQNLN
                     GKLYQVARKDYADRKAWKEIMPHDKDVRIESVVVQKDYLIIETRKNGLANLEIRNLNN
                     GKKEFITFPEPVYTANSNGNPEYDAATFRYTYTSLNRPTTLYEYNCETAKTEKLKQQE
                     IPSGFNPDDYKVERLWATAADGASIPMAVVYKKNLKKNGKNPALLYSYGSYGMSSDVY
                     FSSSFYSLIDRGFVFGIAQIRGGSDMGEQWYEDGKLLKKKNTFTDFIACSEKLIADKY
                     TSADKLAAMGGSAGGLLMGAVANMRPDLYQTIVAQVPFVDVVTTMLDETLPLTTGEYE
                     EWGNPNEEDYFRYMLSYSPYDNVKAQNYPNMLVTGGINDSQVLFHEPSKYTAKLRALK
                     TDDNLLILHMNMDSGHGGATGRYDGIKDTAFELAFILNRVGIKK"
     gene            complement(2074..4146)
                     /locus_tag="PKH68_03330"
     CDS             complement(2074..4146)
                     /locus_tag="PKH68_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX12002.1"
                     /translation="MRPQRIILLISLLWLLISGLRAQDASGNTEQIIADIYEQVSEDT
                     ETEIDFTSLYEDLMALAENPINLNKTNKEELEKLQFLSDTQIENILYYIYKSAPLNTI
                     YELQLIEGLDMTDIRRMLPFVSVGEGESQTRKIRLRDIRRFGKNELMMRFDRGLETKE
                     GYRFLPEEDEQSTAQNAKKYLGDPYYTYLKYRFHYRDRIQAGITAEKDAGEQMWGNEH
                     KGYDFYSAHVELRNFGKLKTLVLGDYRVNFGQGLVIRTDFSMGKSSYVLNVSPRSGGL
                     KKYSSTAESNFFRGSGATLKLGKTELTAFYSNKMLDGDTTNGNFQTIIRDGLHRTAND
                     LLTKGTVNEQVMGGNVSFTHSWFQLGATLVHTRLDHDLQPDEAIYNRFYFRGKNQTAG
                     SVNYRIRWHKLNIFGETAMTEKSAVATINGLNFSPVSRVSLVALHRYFAKNYDTFFAN
                     TFSETSRVNNESGLYIGAEIRPVKFWKISAYVDSYRFFWPKFGINSPSIGNDFLLQAD
                     YFPKRNVAMFWRVKYEEKGHNFTDTLTTMPLVLTQPKWQARYQLNYSFGRFKFKNQLD
                     ANGYSDGVNKPSYGFSALQDVSYSFERLPLSLDIRLHIFDALNFENRFYTYEKDVLYA
                     FSIPMAYGQGTRYYLNLRYDVNSSLSLMFKLAQTVYADDRTTISSSNEEIQGNRKTDF
                     RFLMKWQF"
     gene            complement(4154..4975)
                     /locus_tag="PKH68_03335"
     CDS             complement(4154..4975)
                     /locus_tag="PKH68_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12003.1"
                     /translation="MKKFIYIFLLIYPAIGNINAQIAPIVPSSTSIANASVAVQNDWS
                     AFQNTAALAHIDKLELAMQYENHSMVKEMSWKSAQAGINAKYVNVGVSFSYFGYSLYN
                     EMIAGIGLARNFSDKFSMGLQFNYYSAYFSGGEENRYRSALLPQMGISSRLFPNFTVG
                     FHAFNPFQTNIKTDLTEKRIPSIYSIGTNYEFAKNLAWLTQIDKEVSSNYRLATGFEY
                     TMLQQLTVKLGAYRMDYLVPCFGIGLYLGQLHFDLNGELHPQLGLNTMARMKYRF"
     gene            5203..5817
                     /locus_tag="PKH68_03340"
     CDS             5203..5817
                     /locus_tag="PKH68_03340"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNX12004.1"
                     /translation="MRRYLKIFILLLITFSSAGVVAQDVTVAKDKFSYSTMMEKVILN
                     GDTLFVDYIREVYVFPPMVFTSKNQEKFYWRTVRDVKKALPYARMVSYEITQVNAELL
                     KLPGDRERKQYISQYKKEAFRKYEKELRNLTVNQGKMLMKLIDREYEVNTYELVKGYL
                     GAVPAFFWNSVAGLFGSTLRQDYDGKDKDRIVERVVILVDAGQL"
     gene            5880..6488
                     /locus_tag="PKH68_03345"
     CDS             5880..6488
                     /locus_tag="PKH68_03345"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNX12005.1"
                     /translation="MIGFSLKRFFEKNFSIFQKQKQIKDKKYQIRADIRAKKRQLSEE
                     EKKEAARIVFERIETMLAFQKAKVVFLYWSTPSELPTHEYVRKWSENKIILLPSVSGD
                     KMHLRRYVSSKEMQMGEGNILEPTTDIYKGHFDLAIIPGIAFDRERHRMGRGRGYYDR
                     FLNSKSITTYGVGFDFQVLRKIPVTRRDMKVTSVFSPSKTIE"
     gene            complement(6509..7222)
                     /locus_tag="PKH68_03350"
     CDS             complement(6509..7222)
                     /locus_tag="PKH68_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12006.1"
                     /translation="MPYRRLPNTDQARLRALRTAIQMGEKQSYDKQVVSFKAIHEAQT
                     FLNVFEKQMQLYKQTLESQVNANKQYQQILYNARMYISHFIQVFNLSVIRGEIKKEHK
                     LYYHLNPDTHQVPDLSTETAIQEWGKNIISGENERIRNGGTPVYNPTIAKVQVHFEVF
                     KEYKSTQRIYQSTTTHNWEELVNQRKKADAIILDIWNQVEEHYRNEKPYAKLMKCKKF
                     GLVYYYRRHEKELTPDDDL"
     gene            complement(7319..8296)
                     /locus_tag="PKH68_03355"
     CDS             complement(7319..8296)
                     /locus_tag="PKH68_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNX12007.1"
                     /translation="MYTFEETLQLINSEIEHIDWNKEPPGLYLPIKYVLALGGKRIRP
                     AVTLMSCNLYSDNVMQAMNSAIGLEIFHNFTLLHDDIMDRADMRRGKPTVHKKWNDNT
                     AILSGDVMQIEAYKWMTQSPADKLKEILDLFSRTAAEICEGQQYDMEFENRENVTADE
                     YIEMIRLKTAVLIAAGCVIGARIGGAGAEDARKLYDFGINIGLAFQLKDDLLDVYGSE
                     ANFGKKIGGDILCNKKTYLLIHALKKAKGKDAEELNYWLKNINPEYACEKIKAVTELY
                     NRLSVKDICEEAIDYYYTKAVASLAEVSAPDVKKSELRGFSEKLMSRKD"
     gene            complement(8339..9313)
                     /locus_tag="PKH68_03360"
     CDS             complement(8339..9313)
                     /locus_tag="PKH68_03360"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNX12008.1"
                     /translation="MKRKIAIIAGGDSSELGVSLKSAAGIYSFIDKEKYDCYIITLVG
                     TTWQVEYSDSEKLPIDKNDFSFRLNGEKVLFDFAYITIHGTPGENGILQGYFDLIKMP
                     YSCCGVLAAAMTFSKFTCNQYLKGFGIRVADSILLRQGQTVSDREVIEKIGLPCFIKP
                     NVGGSSFGVTKVKEPVQIQPAIAKAFSEGIEVLIETFTPGKEFTCGMYKTKSKTTVFP
                     VTEIVPKNEFFDFGAKYNNESEEITPARISENLSVRIKTLTEAIYDILGCKGIIRVDY
                     IISEGEIINLLEVNTTPGMTATSFIPQQVRAAGIDIRDVMTDIIEDSF"
     gene            complement(9326..10408)
                     /locus_tag="PKH68_03365"
     CDS             complement(9326..10408)
                     /locus_tag="PKH68_03365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNX12009.1"
                     /translation="MLEKEKDEYSEEFDFPEDVDEVENGPEMFEHFRFEVDKGQSMLR
                     IDKYLVIQMASTSRNRIQDAADAGNILVNGKPVKSNYKVKPLDVISIVMAYPPSSFEI
                     IPQNIPINIVYEDDHIILINKQPGLVVHPGFGNFDGTLLNAIAFHMKDHPDFNVNDAR
                     IGLVHRIDKDTSGLVLIAKTPDAKSRLGKQFFEKTTKRSYNTVVWGNVKEDEGTITGS
                     LARDPRDRMLFKVFGEGENPLAKHAVTHYKVLERLGYVTLVECRLETGRTHQIRVHMK
                     HIGHTIFNDERYGGHEVLKGNRTAKYQQFVRNCFDICPRQALHAKTLGFVHPISGEEM
                     FFESELPDDMQELTEKWRNYAKQLEK"
     gene            complement(10418..11263)
                     /locus_tag="PKH68_03370"
     CDS             complement(10418..11263)
                     /locus_tag="PKH68_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNX12010.1"
                     /translation="MEKNSFWNNNIFGYTLKNLLIAIAVFIGLVWFTLIMIDFYTNHG
                     DTHKVPGVKGVNVEQAMDMLSRLDLKAVVIDSVYVKDKKLGTVIEQNPVEGSVVKPGR
                     PIYLVVNSRSVRLIALPSVTDISIRQAEATLMSIGIKVSSIQYAPSEYKDLVLEVKYR
                     GQSIIQGAKIPEGSSVVLVAGNGYGASATAVPSVLGMNLSAATDIISASSFVPGGVIY
                     DIEPNGDEELYLIYDQRPAAGDSVSSGSTINLWLSKDPAKKAPPRVKKQQVEEEKVQD
                     IEDFF"
     gene            11548..11982
                     /gene="dut"
                     /locus_tag="PKH68_03375"
     CDS             11548..11982
                     /gene="dut"
                     /locus_tag="PKH68_03375"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNX12011.1"
                     /translation="MKINIVNKSRHPLPEYATSHSAGMDLRANIDEPVELKPLERKLI
                     PTGLFIELPEGYEAQIRPRSGLAIKKGITVLNSPGTIDADYRGEVCIILINLSAETFV
                     INDGERICQMIVAPHARAEWDEVALLNETDRGTGGFGHTGKN"
     gene            12020..13786
                     /locus_tag="PKH68_03380"
     CDS             12020..13786
                     /locus_tag="PKH68_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX12012.1"
                     /translation="MIKQHFLLIFSAILLFTACGTQKSAVATTPGNSLTADEQQRFDY
                     YFYEALRLKNTGDPGAQMEALRMCLEIDSTSGAVQSETGHAFAQLNSLPEATRAFRKA
                     VMASPTNWWYRVQLISMLSGTEQYAEAITHAVALSKIYPEREAVYNMLSSLYKQTGEY
                     DKAIYALDKLESFVGINEYLSFEKFQLYAALNKAKKATAEIDRLVEKYPAETRYQVLR
                     GDIFMDQKQPEKAFEVYQQVLASDPSNPFVYVSLANYYKEKNQPEKALESIVSALKNP
                     ALPSDTKMDILGQYVDKLLGDNQKIAETEELFKLLVEMYPLDEMPHAYYAHFLQNQKR
                     MPEALSELESVININPKNEAAWVTSLQILADKEDTVAILDLTERGIRELPKIPQFYFY
                     RSMAQYQQGNYEAALQTNEEALKNVDTAPASVISSFYGQIGDIWYKLSNKEKAFANYE
                     KALEQNPGNVYVMNNYAYYLSEEKLDLRKAERMSGKTVELEPKNSTYLDTYAWILYQQ
                     GNYSLAKFYIERAVDNLEKEHDPGVILEHYGDILQALNDRKKAVEIWQKALDAGITDE
                     ALKAKIEKTKQEIEYENESKNK"
     gene            13767..14582
                     /locus_tag="PKH68_03385"
     CDS             13767..14582
                     /locus_tag="PKH68_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNX12013.1"
                     /translation="MKVKINKVILLSMIILALGACKTKKQLTKTEIPAEMTVAQMVGK
                     LQEKQPAFSSAYIDKMTVDVDVKNRQMTVNATCKMRTDSAIHLSIQPFFGIELFKLEL
                     TPYNIILIDKTNKMYYRGQYSMLRSRFGVDVDYKSIQALISNRLFVAGNRAFLTDDFK
                     WRNDSATSGVLAVQGERTSQEVTVNPALERIIRMVIKTNDNAYTLNTDYSGFKNFDKQ
                     LFPENINIEALNAGEKASFLFRIERVEFDKPLVMEPANLQRYRQGDFSRFLTK"
     gene            14644..16119
                     /locus_tag="PKH68_03390"
     CDS             14644..16119
                     /locus_tag="PKH68_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNX12014.1"
                     /translation="MKNKTRFLLSVILLGFVINLSLQAQTVDDLQRERKQIQERILTT
                     NKQIKQTEKKEKASLSKLDFLKRNLKERKNLINSYGKEISLLDKKINELTAQKKELET
                     QLEKLKQDYAKLIQKTQANRSSYSKLMFLLSSNNFDQTLRRVRYLQEFTNYRKEQAQR
                     IEKVKLEIALKTDSLDTHKGSKSRALKAKELEAAKLKQDENNEKVMLASLQQEEKKLR
                     DEYRIQQSKRNQIDNKIEQIIAEEIRKAEARRRAEAAQKQAEELRKKLAEERKIKEER
                     DRKAAEERKLAEAKKAKEKTSTSTTAKTETSKAAETPVIAEATTKTAETVAKSAETAR
                     ESSGTSAVVYEMSREESLLSGGFEQNRGRLPWPVERGSITGHYGVQPHPVLKHVQIDN
                     KGVYFQSPAGTNARAVFDGVVERRFSLPGSGNAIIIQHGNYRTLYGNLTNIYVRTGDR
                     VKAKQAIGQIYTDDENGGKTELIFQVWSGLTRLNPENWISR"
     gene            16170..>17985
                     /locus_tag="PKH68_03395"
     CDS             16170..>17985
                     /locus_tag="PKH68_03395"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNX12015.1"
                     /translation="MENLKEQEVAEITYDDANIVTLEGLEHIRRRPGMYIGKLGDGTH
                     SDDGIYVLLKEVIDNSIDEYRMGYGKTIEVGINDGVVSVRDYGRGIPLNSLVKAVSIM
                     NTGGKFDSQAFKKSVGLNGVGTKAVNALSSRFVIQSFREGQSRRAEFECGILQAEELE
                     AVKADNGTLVVFQPDRELFQNYVYNDDIIETMLRNFAYLNTGLTILFNGKKILSKNGL
                     TDLLNENMTAEPLYPIIHLKAEDIEIAITHSDQYGEEYYSFVNGQHTTQGGTHQSAFR
                     EAVAKTIKEFYNKNHELTDIRNGIIAAVAIGVEEPVFESQTKTKLGSRDMSKNGITVN
                     KFIADFVKKELDNYLHRNSETADILLQKIQESEKERKAIAGVTKLARDRAKKVNLHNK
                     KLRDCRIHYNDTKGSPELLDSSSIFITEGDSASGSITKSRDVNTQAVFSLRGKPLNSF
                     GLTKKIVYENEEFNLLQAALNIEDGIDGLRYNKVIVSTDADVDGMHIRLLLITFFLQF
                     FPDLIKKGHVYILQTPLFRVRNKKETIYCYSEEERLAAMARLGANPEITRFKGLGEIS
                     PDEFKHFIGKDIRLDQVTLKKEDAVMDLLAFYMGKNTSE"
BASE COUNT         5281 a         3862 c         3968 g         4874 t
ORIGIN      
        1 tttatcaatt tcgggcaaac acgagtggac aattactact ggatgaagga taagaataat
       61 ccgaaagtga ttgattacct gaaagctgaa aatgcctata ccgatacggt gatggcttca
      121 accaaagatc ttcagaaaaa aatatacgac gaaatcattg gtcgtatcaa ggaagatgac
      181 cagtcgtatc cgaccctgtc taatgggtat tactactata cccgtaccga aaaaggcaag
      241 cagtaccgca cctattgccg taaaaacgga tcagaaaaag cgcccgaaga aatcattttt
      301 gatgtcaata agatggccga aggcaaacct gcttttatgt ttggcggata cgatgtaagt
      361 cccgacaacc aacttgccgg atatttttac aacgagacgg gttcgtttgc cgattatgta
      421 ctgaaaataa aagatctgaa aactggaaaa gatatggact tcgccgtgaa aggtgcttcc
      481 tcatttgcct gggccaacga caataagacc ttgttctaca gtgtgataga tccggtcaca
      541 ctgaggtcga accgcgtatt tcgtcaggtg cttggcaatc cgaaagcggt gcttatttac
      601 gaagaaaagg ataccaaatt ttccctcggt gttaccgaaa ctaaaacccg ggatttcatt
      661 tttatcaaca ccggcagtac gaataccacc gaagaactat ttgtaccggc cgacaaacct
      721 gccgaagcat ttaagatttt tatgccccgg gtaaaggatg tggactattc tgtttttaca
      781 cacagggact actttttcat tcgatataag gacaagcaaa atctgaacgg aaaactctac
      841 caggtggctc gaaaagacta tgccgaccgc aaagcatgga aagaaatcat gccacatgat
      901 aaagatgtgc gcatcgaaag tgtggtggtt cagaaagact atctcatcat cgaaacccgc
      961 aaaaacggac tggcaaatct tgaaattcgt aatctcaaca acgggaaaaa agaatttatc
     1021 accttccccg aaccagttta tacagccaat tccaacggaa acccggagta cgatgcagcc
     1081 actttccgct atacatatac ttcactcaac aggcctacaa ccctgtatga atacaactgc
     1141 gaaactgcca aaaccgaaaa gctgaagcaa caggaaattc catccggttt caatcccgac
     1201 gattataagg tggaaagact ttgggctacg gctgccgatg gagcaagtat cccgatggct
     1261 gttgtttaca aaaaaaatct gaagaaaaat ggcaaaaatc ccgctttgct ctactcttac
     1321 ggtagttatg gtatgagttc ggatgtatat ttcagcagca gtttttacag cctgatcgac
     1381 cgcgggtttg tatttggtat tgcccagata cgtggcggca gtgatatggg cgaacaatgg
     1441 tacgaggacg gtaaactcct gaaaaagaaa aatacattta ccgattttat tgcctgcagc
     1501 gaaaaactga tagccgataa atacacttca gccgacaaac tggccgccat gggaggcagt
     1561 gccgggggct tgctgatggg tgccgtggcc aatatgcgac ccgatcttta tcagaccatc
     1621 gtggctcagg tgccgtttgt ggatgtggtg actaccatgc tcgacgaaac tcttccgctc
     1681 accaccggcg agtacgaaga atggggaaat ccgaacgaag aagattattt ccgctacatg
     1741 ctttcgtatt caccttatga taatgtaaaa gcacagaatt atcccaatat gctcgtaacc
     1801 ggtggtatca atgactctca ggttttgttt cacgaaccga gtaaatacac tgccaaactt
     1861 cgggcactga aaaccgatga taatctgttg attttgcaca tgaatatgga ctccggacat
     1921 ggcggagcta ccgggcgata cgatggaata aaggacactg cctttgaact ggcatttatc
     1981 ctaaaccggg taggtattaa aaaataataa acgactaatt tgccatcaga aacgtcaggt
     2041 atccattctc ctgacgtttt tgttttttta ctcttaaaac tgccatttca tcaaaaaccg
     2101 gaaatccgtt tttcggtttc cctgaatttc ttcattgctg ctgctgatgg tcgttcggtc
     2161 gtcggcatac acggtttggg cgagtttgaa catgagtgac aggctcgagt taacatcgta
     2221 ccgcagattg aggtaatacc gtgtgccttg tccgtaagcc atggggatgg aaaatgcata
     2281 caatacatct ttttcgtagg tgtagaagcg gttttcgaaa ttgagagcat caaaaatatg
     2341 caaccggata tccagactca atggcaaccg ctcaaaactg taactcacat cctgtaaagc
     2401 cgaaaatcca tacgatggtt tattcactcc atcgctgtat ccgtttgcat ccagttgatt
     2461 tttgaatttg aagcgtccga acgaataatt cagttggtaa cgtgcctgcc atttgggttg
     2521 ggtgagtaca agcggcatag tagtcagggt atctgtgaaa ttatgtcctt tttcctcata
     2581 ttttacccgc caaaacatgg ctacatttcg ttttgggaaa taatcggcct gcaggaggaa
     2641 atcattccct atggaaggtg aattgatgcc gaatttcggc cagaagaatc gataactatc
     2701 cacataagct gaaatcttcc agaattttac aggacggatt tcagcaccga tatataaccc
     2761 gctttcgtta ttgacacggg atgtttccga aaatgtattg gcaaaaaaag tatcgtaatt
     2821 tttggcaaaa tagcggtgta atgccaccag acttacccgc gatacgggac tgaaattcag
     2881 accgttgata gtagccacgg ctgatttttc ggtcatggct gtttcaccga aaatattcaa
     2941 tttatgccag cgtatgcggt agttgacact tccggctgtc tggtttttac ccctgaagta
     3001 aaaccggtta taaatggctt cgtcgggttg caggtcatga tcgagccggg tgtgcaccag
     3061 ggtggcgccc aactgaaacc acgaatgggt gaaactcacg tttccaccca tgacctgttc
     3121 gttgaccgtt ccttttgtca gcaagtcgtt ggctgtgcgg tgaagtccgt cgcggataat
     3181 ggtctgaaaa ttaccgttgg tggtatcacc atcgagcatt ttatttgaat aaaaagccgt
     3241 aagttcagtt ttaccaagtt ttaatgttgc acctgaacct ctaaaaaaat tggattcggc
     3301 ggtagaactg tactttttca aacctcccga acgtggcgat acatttagaa cgtaggatga
     3361 tttacccatc gaaaaatcgg tgcgaattac cagtccctgc ccaaaattaa cccggtaatc
     3421 acccagcacc agggttttca attttccgaa atttctcagt tcaacatgag ccgaatagaa
     3481 atcatagcct ttgtgctcat ttccccacat ttgttcaccg gcatcttttt cggcagtaat
     3541 tccggcctgt atgcggtcgc ggtaatgaaa gcgatatttc aggtaagtgt aatatggatc
     3601 acccaggtat ttttttgcat tttgagcggt tgattgttcg tcttcttcgg gcagaaaccg
     3661 gtaaccttcc ttggtctcca gtccacgatc gaacctcatc atgagttcat ttttaccaaa
     3721 acggcggata tcccgcagtc tgattttccg ggtttgactt tccccttcac ccacacttac
     3781 aaacggaagc atccggcgga tgtcggtcat gtccaatccc tcaatcagtt gcaactcgta
     3841 gattgtattc agcggagcac ttttataaat atagtacagg atgttttcga tctgagtatc
     3901 ggacagaaac tgtagttttt ccagttcttc cttattggtt ttattcagat taattgggtt
     3961 ctcagcgagc gccataaggt cttcgtagag gcttgtgaag tcaatttctg tttcggtatc
     4021 ttccgaaacc tgctcgtaga tatcggctat gatctgttcg gtattacccg atgcatcttg
     4081 cgcccgcaat ccggatataa gtaaccagag cagagagatc agcagtatga ttcgttgcgg
     4141 tctcataatt gtttcagaac cggtatttca tacgtgccat tgtgttcagt ccgagttgtg
     4201 ggtgcagctc gccgttcagg tcgaaatgta actgaccgag gtatagccct attccgaaac
     4261 agggaaccag ataatccatc cggtaagcac ccagtttcac agtgagttgt tggagcatgg
     4321 tgtactcaaa tccggtagca agccggtaat tactactcac ttccttgtca atttgcgtga
     4381 gccacgccag attttttgca aattcgtagt tggtaccgat gctgtaaatg gacggtattc
     4441 ttttttcggt taaatcggtt ttgatgtttg tctgaaatgg attgaaagca tggaaaccta
     4501 cggtaaaatt tggaaacagg cgggatgaaa ttcccatttg gggcaacagt gcgcttcggt
     4561 agcggttttc ttcgccgccc gagaaatatg cactgtagta gttgaattgg agtcccattg
     4621 aaaatttatc gctaaagttg cgcgccagtc caatccctgc aatcatttca ttgtaaagcg
     4681 aatagccaaa ataggaaaaa gaaactccaa catttacata tttggcattg attcctgcct
     4741 gagcactttt ccagctcatt tccttgacca tcgaatgatt ttcgtactgc atggccagtt
     4801 ccagtttatc gatgtgagcc agtgcggctg tattctgaaa agcactccag tcgttctgta
     4861 cagctacaga tgcattggct atggaggtag acgagggaac aatcggcgct atctgtgcgt
     4921 taatattacc gatagcagga tatataagca gaaaaatgta gataaatttt ttcatcaatt
     4981 acatttatat gttgtaaaac ggcgatttac agggcttaac tgaaatatca attatttata
     5041 tcaggataaa agtaattgtt ttttccaaaa catagaaata aatttaaaaa atgtttattc
     5101 taaaaaaaat aatttggaat aaaaattgtt atatacttgt ggaacttgca aataacgctt
     5161 atttttgcac cacaatattg tatgctaata aaaattagtg aattgagacg atatctgaaa
     5221 atatttatac tgctcctgat tacattctcc tccgctggag tcgttgccca ggatgttact
     5281 gtcgctaagg ataagttttc atattccaca atgatggaaa aagtaatcct gaatggagat
     5341 acactttttg tggactatat cagagaggta tatgtatttc cgcccatggt ttttaccagc
     5401 aaaaatcagg aaaaattcta ctggcgaacg gttcgggatg taaaaaaggc acttccgtat
     5461 gcccgtatgg tttcgtatga aatcacccag gtgaacgcag aattattgaa attgccgggt
     5521 gacagggagc gtaaacaata catcagtcag tacaaaaaag aagccttccg aaaatacgaa
     5581 aaagaactgc gaaacctgac ggtaaatcag ggaaaaatgc tgatgaaact catcgatcgc
     5641 gaatacgagg tgaataccta cgaattggta aagggttatc tgggtgctgt acctgctttt
     5701 ttctggaatt cggtggctgg attatttgga tctacgctca ggcaggatta tgacggaaag
     5761 gataaagacc gtatagtgga gcgtgtggtg atactggtgg atgccggtca actttaactg
     5821 taaatgttca gaaatatttg tttacccgat ataaaccttt gtttgaaaaa gatattagta
     5881 tgattggatt ttcccttaaa cgattttttg aaaagaattt cagtattttc caaaaacaga
     5941 aacaaataaa agataagaaa tatcaaattc gtgccgatat ccgcgccaag aaacggcagc
     6001 tatccgaaga ggaaaaaaag gaagctgccc gaattgtatt tgaacgaatt gaaacgatgc
     6061 ttgccttcca aaaggcgaaa gtcgtatttc tttactggtc taccccctcg gaacttccca
     6121 ctcacgaata cgtaagaaaa tggagcgaga acaaaattat tttgctcccc tcggtttcgg
     6181 gtgataaaat gcatctgcgc cgatatgttt cgtccaaaga aatgcagatg ggcgaaggta
     6241 atattctgga acctaccacc gatatttaca agggacattt tgatctggcc attattccgg
     6301 gcatcgcttt tgaccgggaa cggcaccgta tgggacgcgg acgaggctac tacgatcgat
     6361 ttctcaattc aaaaagtatt acaacgtatg gtgtgggatt tgattttcag gtattacgaa
     6421 agattcctgt tacccgtcgg gatatgaaag ttaccagcgt gttcagtccc agcaaaacaa
     6481 tagagtaaac agccactggc tgagggattt acaggtcatc atcgggtgta agttcctttt
     6541 cgtgccgtcg gtaataatac accaatccga attttttgca tttcatcagt ttggcatacg
     6601 gtttttcgtt ccggtaatgc tcctctacct gattccaaat atccagaatg atggcatctg
     6661 cttttttccg ctgattgacc agttcctccc agttgtgagt ggtggtgctt tgatagatgc
     6721 gttgagtgga tttatattcc ttgaaaacct cgaaatgaac ctgcactttt gctatggtgg
     6781 ggttgtatac aggggttccg ccattcctga tgcgctcatt ttcaccgctg atgatgtttt
     6841 ttccccattc ctgaatggct gtttcggtgc ttaaatccgg aacctgatga gtgtcggggt
     6901 tcaggtgata atacagtttg tgctcttttt ttatttcccc ccgtatgacc gaaagattga
     6961 acacctgaat gaaatgtgag atgtacattc gggcattgta taaaatctgc tgatattgtt
     7021 tgttggcatt gacctgactt tcgagagttt gcttgtagag ttgcatttgt ttctcaaata
     7081 cattgagaaa tgtctgagct tcatgaatgg ctttgaagga caccacctgc ttgtcgtaac
     7141 tttgcttttc gcccatttgt atggcggtgc gtaaagctct tagtcgggcc tgatcggtat
     7201 ttggcaagcg gcggtagggc atctggttag ttgaaaatag ttaattccgg gctgaaagtt
     7261 agttgtttac agggggttat ttcgcactaa ataacgtttg taagcaaaag tacataattt
     7321 aatctttacg tgacatcagt ttttctgaaa atcctcttaa ttcggatttt ttaacgtcgg
     7381 gagctgagac ctctgcgagt gatgctactg ctttggtgta gtagtaatca atcgcttctt
     7441 cgcaaatatc tttgactgat agccggttat agagttctgt cactgcttta attttttcgc
     7501 aggcatattc cggattaata ttctttaacc aataattcag ctcttcagca tcctttcctt
     7561 tggctttttt aagggcatga atgagtaggt aagttttttt attacacaga atatcgcctc
     7621 cgattttttt tccgaaattg gcttcactgc catatacatc aagcagatcg tcctttagct
     7681 gaaatgcaag tccgatattg atgccgaagt catagagttt tcgggcatct tctgcaccgg
     7741 caccgcctat acgggcacct atcacgcagc cggcggcaat cagcacagcc gttttcagcc
     7801 ggatcatttc tatatattca tcagccgtaa cattctcacg attctcaaat tccatgtcgt
     7861 attgctgtcc ttcacatatt tcagcagccg ttctcgaaaa aagatcgaga atttccttca
     7921 gtttgtcagc cggtgattgg gtcatccatt tataggcttc aatctgcatc acatcgcccg
     7981 aaagaatagc tgtgttatcg ttccattttt tatgtaccgt tggttttccg cgtcgcatat
     8041 cggcacgatc catgatatcg tcgtgcagaa gggtgaaatt gtgaaatatc tccagcccga
     8101 tggccgaatt catggcctgc atgacgttat ccgaatacag attgcatgac atcagagtga
     8161 cagcaggccg tattctttta ccgccgagtg ccagtacata ttttattggc agataaagtc
     8221 ccggaggttc tttgttccag tctatatgtt cgatttccga gttaatcagt tgaagtgttt
     8281 cttcgaaagt gtacatggcc ttaatttttt acatgattag gtaggcagtg aacggaaatt
     8341 aaaagctatc ctcaatgata tcggtcatca catcacggat atcgataccg gctgcccgca
     8401 cttgttgagg aatgaaactg gtggcagtca taccgggtgt ggtgttcact tccagcaggt
     8461 taataatttc tccttcggaa ataatatagt ccacccggat gatacctttg cagcccagta
     8521 tgtcgtagat ggcttcggtc agcgttttga tgcgtactga caaattttcg gaaatccggg
     8581 cgggagtaat ttcttccgat tcgttgttgt atttagctcc gaaatcaaaa aattcattct
     8641 ttggtacaat ttcagttacc gggaaaaccg ttgtttttga tttggttttg tacattccgc
     8701 aggtaaattc tttgcctggg gtgaacgttt ctatcagcac ctcaatgcct tcgctgaatg
     8761 cttttgcaat ggcaggttgt atttgcaccg gttcttttac ttttgttaca ccaaaactgc
     8821 tgccacctac atttggtttg atgaagcagg gtagtccaat tttttcaatc acttcccggt
     8881 cgcttacagt ttgtccctga cgcagtaata ttgaatcggc cacgcggata ccaaatcctt
     8941 tcaggtactg attgcaggta aatttgctga aagtcatggc ggcagccaat acaccgcagc
     9001 aagaataggg catttttatc aaatcgaaat agccttgcag aatgccgttt tcgccgggtg
     9061 taccatggat ggtgatgtag gcaaaatcaa acagcacctt ttcaccatta agtctgaaac
     9121 tgaaatcatt cttatcaatc ggcagctttt cgctatcgct gtattccacc tgccaggtag
     9181 ttcccaccag ggtgatgatg tagcaatcgt atttttcttt gtcaataaag gaataaatac
     9241 cagctgcact tttgagcgac actcccagtt cggatgagtc gcctccggct atgattgcaa
     9301 tttttctttt catatgtata atagcttatt tttcaagttg tttggcgtaa tttctccatt
     9361 tttcggtcag ttcctgcatg tcgtcgggca attcgctttc gaaaaacatt tcttcaccac
     9421 ttatgggatg aacaaatccc agtgttttgg cgtgcagtgc ctgtcgcgga cagatatcaa
     9481 agcagttgcg aacaaattgc tgatatttgg cagttcgatt tcctttcaga acttcgtggc
     9541 ctccgtatcg ctcgtcgtta aagatggtgt gaccaatgtg tttcatgtgc acgcgtatct
     9601 ggtgtgtgcg accggtctcc aggcggcatt ctaccagtgt tacatatccc agccgctcca
     9661 gtaccttgta gtgcgttaca gcatgttttg ccagtggatt ttcaccctct ccgaatacct
     9721 taaaaagcat tctgtcacgc gggtcgcgcg caagactgcc tgttatcgtg ccctcatctt
     9781 ccttcacatt tccccatacc acagtgttgt acgaccgttt tgtggttttt tcaaaaaact
     9841 gttttcccag tctcgacttg gcatcgggtg ttttggctat aagtaccagt cctgatgtgt
     9901 ccttgtcgat tcggtgaacc agtccgatgc gggcatcgtt gacattaaag tcgggatgat
     9961 ctttcatgtg aaaagcaatg gcattgagca gtgtgccgtc aaagttgcca aaacccggat
    10021 gaacaaccag tcccggttgt ttattaatca gaatgatatg gtcatcttca tacacaatat
    10081 tgatgggaat attctggggt ataatttcaa aactggaggg tgggtaggcc attacaatag
    10141 aaataacatc cagcggcttc accttgtagt ttgattttac cggttttccg ttgaccagaa
    10201 tattgcccgc atcggcagca tcctgtatgc ggttgcgcga tgtcgaagcc atttgtatga
    10261 ccagatattt gtcaatgcgg agcatcgact gccctttgtc cacttcaaac ctgaaatgtt
    10321 caaacatttc gggtccattt tccacctcat caacgtcctc ggggaagtca aattcctcag
    10381 aatattcgtc tttttctttt tccaacatac tcctcgctta aaagaaatcc tcaatatcct
    10441 gaactttttc ttcttctacc tgctgtttct ttaccctggg tggtgctttt ttagcaggat
    10501 ctttgctgag ccacaggtta atggtactgc ccgatgacac cgaatcgccg gctgcaggac
    10561 gctgatcata tatcaaatac aattcctcgt caccgttggg ctcgatatca taaattacgc
    10621 ctcccggaac aaaggaagaa gcactgatga tatcagtggc tgcagaaaga ttcatgccca
    10681 ataccgaagg aactgccgta gctgaagctc cgtagccgtt accggcaacc agtaccaccg
    10741 aactgccttc ggggattttg gcaccctgaa taatgctttg accgcggtat tttacttcga
    10801 gcaccagatc tttgtattcg gagggagcat attgtatgga actcactttg ataccaatcg
    10861 acataagtgt ggcttcagcc tgacgtatag aaatgtcggt gaccgaaggc aatgctatga
    10921 ggcgaacaga acgggaattg acaaccagat aaatgggacg accgggtttt actactgatc
    10981 cttcaaccgg attttgctca atcacagtac caagtttttt gtctttgaca taaaccgagt
    11041 ctattaccac agcttttaag tccaaccggg aaagcatatc catcgcctgc tctacattca
    11101 cccctttcac cccgggaact ttgtgggtgt caccgtggtt ggtgtagaaa tcaatcatta
    11161 taagcgtgaa ccataccaaa ccgataaata cggcaatggc tatcaataag ttctttaatg
    11221 tataaccgaa aatattatta ttccagaaac tgtttttctc catgtaatgt cactgtttag
    11281 cagttgagac atcaggtgtt gatgtttttt ttattaataa tgcaaaatta ctgaaattat
    11341 tgcagtaatt attcgtttat caaaataaat atcgggaccg aatagatgaa tactgttgag
    11401 tttaaccatt tttttcaatt taataaatgg gaaaattaga tattttaagc aataaagtaa
    11461 aagcctttcg cattttatgt cgtatttttg caagttaaaa taaaaaaata attttcgttg
    11521 gtcattaaca aaaaagccaa gtaatatatg aaaatcaaca tagtaaataa gtcccgccat
    11581 ccactgcccg aatatgccac atcgcattcg gccggtatgg atctgagggc aaatattgat
    11641 gaaccggttg aattgaagcc ccttgaaaga aagttgatcc ctaccggact gttcatcgaa
    11701 ctgcccgaag gatatgaggc acagatacgc ccccgaagcg gactggctat caaaaaggga
    11761 attactgttt tgaattcgcc cggaaccatt gatgctgatt ataggggaga agtttgcata
    11821 atactgatta acctctccgc cgaaacattc gtaatcaatg acggggaacg gatttgtcag
    11881 atgatagttg ccccgcatgc acgggctgaa tgggatgaag tcgctctgct gaatgaaacc
    11941 gatcggggaa caggtggatt tggacatacc ggcaaaaatt aaatgaataa taaaaaactt
    12001 taccggcaga ttttttagaa tgataaaaca acactttctc ctcatatttt ccgctatttt
    12061 gcttttcaca gcttgtggta cgcagaaatc tgccgtggct actacacccg gaaattccct
    12121 gactgccgat gagcaacagc ggttcgatta ttatttctac gaagccctgc ggttgaaaaa
    12181 caccggcgat cccggtgcac agatggaagc attgcgcatg tgtctggaga ttgactctac
    12241 cagcggtgca gttcaaagtg aaaccggtca tgcttttgca caacttaata gtttgcctga
    12301 ggccaccaga gcttttagaa aagcagtaat ggcaagtccg accaactggt ggtacagggt
    12361 gcagctcatt tcaatgcttt cgggcaccga acagtatgcc gaagcaatca cccacgccgt
    12421 ggcgctcagc aagatttacc ccgaacgcga agccgtgtac aatatgctct cttcgctcta
    12481 caagcagacc ggtgagtacg ataaagccat ctatgcactc gacaaactgg agtcttttgt
    12541 gggaattaat gaatatcttt cctttgaaaa atttcagctc tatgctgcgc tcaataaggc
    12601 caaaaaagca accgccgaaa tagaccgctt agtggagaaa tatccggctg aaacccgcta
    12661 ccaggtactc cgtggcgata ttttcatgga tcaaaagcaa cccgaaaaag cattcgaagt
    12721 atatcagcag gttcttgcca gtgatccctc caatcccttt gtatatgtgt cgctggccaa
    12781 ttactacaaa gaaaaaaatc aacccgaaaa agccttggaa tcaattgtat ctgcattgaa
    12841 aaatccggcc cttccgtctg ataccaaaat ggatattctg gggcaatatg tcgataaatt
    12901 gttgggcgac aatcagaaaa ttgccgaaac cgaggaactg ttcaaactgc ttgtggagat
    12961 gtatccgctc gacgaaatgc cgcatgctta ttatgcccat tttctacaaa atcaaaaaag
    13021 aatgcccgaa gcactctcgg aactcgaaag tgtgattaat attaacccca aaaacgaagc
    13081 tgcctgggta acttcgctgc aaattctggc tgataaggaa gacactgttg ccatactcga
    13141 cctcacagaa cgtggcatca gggagttacc caaaattcca cagttttatt tttatcgttc
    13201 catggctcag tatcaacagg gcaattatga agctgcactc caaacaaacg aagaggcgct
    13261 gaaaaatgtg gatactgcgc ccgcttcggt tatcagcagt ttttacggtc agattggcga
    13321 tatctggtac aagttgagca ataaggagaa agctttcgcc aattacgaaa aagccctgga
    13381 acaaaacccc ggcaacgtct acgtgatgaa taattacgcg tactatctct ccgaggaaaa
    13441 acttgatctg cgaaaagcag aacgcatgag tggcaaaacg gtagaactgg agcccaaaaa
    13501 cagtacttat ctggatacct atgcctggat attgtaccag cagggcaact acagcctggc
    13561 taaattctac attgagcggg cggtggataa cctcgaaaaa gaacacgatc cgggagtaat
    13621 actcgaacat tacggcgata ttcttcaggc actcaatgac cgtaaaaaag cagttgagat
    13681 atggcaaaaa gccctggatg caggaattac ggatgaagca ttgaaagcaa aaattgaaaa
    13741 aacaaaacag gaaattgaat atgaaaatga aagtaaaaat aaataaggtc atacttttga
    13801 gtatgattat tctggctttg ggagcctgca agaccaaaaa acagttgaca aaaaccgaaa
    13861 taccggcaga aatgacagtt gcccaaatgg taggaaagtt gcaggaaaaa caaccggcat
    13921 tctcctcggc ctatatcgat aagatgaccg tggatgtgga tgtgaaaaac cgtcagatga
    13981 ccgtaaatgc gacctgtaaa atgcggaccg attcagccat acacctctcc atacagcctt
    14041 ttttcgggat cgaattgttc aaactggaac ttactcccta caatatcatt cttattgaca
    14101 aaacaaataa aatgtattac cgcgggcagt acagtatgct ccgcagtcgt tttggagtgg
    14161 atgtggatta taaaagcatt caggcgctta taagcaatcg tctgtttgtg gctggcaacc
    14221 gggcatttct gaccgatgat ttcaagtgga gaaacgacag cgcaacttcc ggcgtacttg
    14281 cagtgcaggg cgaacgtacc tcccaggaag taactgtcaa ccctgctctg gagcgaatta
    14341 tacgtatggt tatcaaaacc aacgataatg catacactct gaatacagat tattccggat
    14401 ttaaaaattt tgacaaacaa cttttccccg aaaatataaa catagaggca ctgaatgcgg
    14461 gagaaaaggc ctcgttccta ttcaggattg aacgtgtaga gtttgataaa ccactggtca
    14521 tggaacccgc caatctccag cgataccggc agggtgactt cagtcgtttc cttacaaaat
    14581 agtgctgata acgggtattg gatatagata gtttttttat tgaaataaaa agatgtaatc
    14641 gtaatgaaga ataagacaag atttctactc tctgtaattt tacttggttt tgttatcaat
    14701 ttgtcgttgc aggcacaaac tgtggacgac ctgcaaagag aacgcaaaca aattcaggaa
    14761 cgaattctta caaccaataa gcagatcaaa caaaccgaaa aaaaagaaaa ggcttcgctg
    14821 agtaaactcg attttctgaa gcggaattta aaagaacgga aaaatctgat taatagttac
    14881 ggaaaagaaa tcagcctgct cgacaagaaa attaatgaac tcacagcgca gaaaaaagaa
    14941 ttggaaactc agctcgaaaa gctgaaacag gattatgcta agctcataca gaaaactcag
    15001 gccaaccgca gctcgtattc caaactgatg tttctgcttt cttcaaataa tttcgaccag
    15061 acgctgcgac gtgtaaggta tctgcaggaa ttcaccaact accgtaagga gcaggcacag
    15121 cggatagaaa aggtcaaatt ggagattgca cttaaaactg atagtctgga tactcacaaa
    15181 ggttccaaat ccagagcgtt aaaggctaaa gaactggaag ccgccaaact caaacaggat
    15241 gaaaacaatg aaaaagtgat gctggccagc ctgcaacagg aagaaaagaa acttcgggat
    15301 gaatatcgca ttcaacaatc caaaagaaac cagatagaca ataaaataga acagatcata
    15361 gccgaagaaa tccgaaaggc agaagcaaga cgacgtgccg aagcggccca aaaacaagcc
    15421 gaagaacttc ggaaaaaact ggcagaagag cggaaaataa aagaagaacg tgaccgcaaa
    15481 gcagccgaag aacgcaaact ggcagaagca aaaaaagcaa aagagaaaac ttcgacgtca
    15541 actacggcta aaactgaaac ttcgaaagcc gctgaaaccc cggtgatagc cgaggcaacc
    15601 acaaaaacag ccgaaacggt tgcaaaaagt gctgaaaccg cccgcgaaag ttcaggtacg
    15661 tcggcggtgg tatatgaaat gtcacgcgaa gaaagtctgc tctccggtgg atttgaacaa
    15721 aaccgcggac ggctgccctg gcctgtggaa cggggtagta tcaccggaca ttacggagtg
    15781 cagccacatc cggtgctcaa acacgtgcaa atcgacaaca aaggggtata ttttcaatct
    15841 cccgccggga caaatgcccg ggctgtgttc gatggagtag tggaacggag gttttcgctc
    15901 cccggcagtg gaaatgcaat tatcatacag cacggaaatt accgcacact atacggaaac
    15961 cttacgaata tttatgttcg tacaggcgat cgggtgaaag ccaaacaagc tattggtcag
    16021 atatatacgg atgatgaaaa cggaggaaaa acagaactga tattccaggt gtggtccgga
    16081 ttgacccgat tgaatcccga gaactggatt tcccgataac cctcaaaaat aacagttgaa
    16141 ttcagaagtt tttttattga tttggaaata tggaaaattt gaaagaacag gaagttgccg
    16201 aaatcacata cgatgacgcc aatattgtaa ccctcgaagg actggaacat atacgccgcc
    16261 gacccggtat gtatatcggc aaactcggtg atggtacgca ttccgatgat ggtatttatg
    16321 tgctactcaa agaagtgatt gacaattcga tagatgaata tcggatgggt tacggtaaaa
    16381 ccattgaagt ggggattaat gatggtgtgg tgagtgtaag ggattatggg cgcggtattc
    16441 cactcaatag cctggtaaaa gcagtttcca tcatgaacac cggaggtaaa ttcgattcac
    16501 aggcattcaa aaaatcggtt ggcttgaacg gagtgggaac taaggcagtg aatgcactct
    16561 catcacgttt tgtaatccag agttttcgtg aaggtcagtc gcgcagggcg gagtttgaat
    16621 gcggcatcct gcaggctgaa gagctggaag ccgttaaagc cgataatggc acactggtcg
    16681 tcttccaacc cgaccgcgag ctgttccaga attatgtata caacgacgac atcatcgaaa
    16741 cgatgcttcg taactttgcc tacctcaata caggactaac catcctgttc aacggaaaaa
    16801 aaatactctc gaaaaacgga ttgaccgatc tgctcaacga gaacatgact gccgagccgc
    16861 tttatcccat cattcacctc aaagccgaag acatcgaaat tgccatcaca cacagtgatc
    16921 agtatggtga agaatactat tcattcgtca atggccaaca taccacgcag ggtggaactc
    16981 atcagagtgc attccgcgaa gcggtagcca aaaccatcaa ggaattttac aacaaaaatc
    17041 acgaactcac cgatattcgt aatggtatta tcgccgctgt ggctatcgga gtggaagaac
    17101 ccgtatttga gtcgcagacc aaaacaaaac tcggctcacg cgatatgtcg aaaaacggca
    17161 tcacggtcaa taaatttatc gctgattttg ttaaaaagga gctcgataac tacctgcacc
    17221 gaaacagcga aacagcggat attctattgc aaaaaattca ggaatccgaa aaagaacgaa
    17281 aagcaatagc cggagtgacc aagctggcac gcgacagagc caaaaaagtg aacctccaca
    17341 ataaaaaact acgcgactgc cgcatacatt acaatgacac gaagggaagc cccgaactgc
    17401 tcgatagcag cagcatcttt atcaccgagg gcgattcggc cagtggttcc attaccaaaa
    17461 gccgcgatgt gaatacacag gcggtgttca gccttcgtgg aaaaccactc aacagtttcg
    17521 gtctgactaa aaaaatagtt tacgaaaacg aagaatttaa tctcctgcaa gctgccctga
    17581 atatcgaaga cggcatagac ggattgcgat acaacaaagt aattgtatcc accgatgccg
    17641 atgtggacgg tatgcacatt cgcctgctcc tcatcacctt tttcctgcag ttttttcccg
    17701 atcttatcaa aaaaggtcac gtttatattc tgcagacgcc acttttcaga gttcgtaata
    17761 aaaaagaaac aatttattgc tattccgaag aggagaggct ggccgctatg gctcggttgg
    17821 gtgccaatcc cgaaatcacc cggttcaaag ggttgggcga aatctcacct gacgaattca
    17881 agcattttat aggtaaggat atccgactcg accaggtaac gttgaaaaaa gaagatgcag
    17941 tgatggatct gttagctttt tatatgggta aaaacacttc cgaac
//
LOCUS       DAOPZK010000033        17950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3290_length_17950_cov_23.325063, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000033 DAOPZK010000000
VERSION     DAOPZK010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17950
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3290_length_17950_cov_23.325063"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKH68_03400"
     CDS             complement(<1..664)
                     /locus_tag="PKH68_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12016.1"
                     /translation="MKSYNTKTLFIRYYLPIFLVFSISVLAFQYQREKKYKADVLDMR
                     LEDTNAAIFYFLEHSGGSLNLLDSLIKNSTFKDERITIIDLSGKVVYDDVVRDVSKMD
                     NHLSRNEVMEARKKGSGSDIRTSKTNRVPYYYHATRFDNCYVRSSLPFNVNVSSVLSP
                     DNLFLYFWLVLTFIVIAILFYFSNRFTIQMRHEQMEQNSLVRRKLTQQVAHELKTPLS
                     SII"
     gene            complement(782..1270)
                     /locus_tag="PKH68_03405"
     CDS             complement(782..1270)
                     /locus_tag="PKH68_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNX12017.1"
                     /translation="MKKLTALLFAIMLCTAMGQAQNLNRFFRKYAKNKQVEYTNVNTG
                     KFFSSILSGISVDTGGELPILNGLKILTLTEDSTNLKLTSKFAGDLHKIIDKGKFDTI
                     FSTRSKTNKTYIYKRLKSRGKADVMIVTKNGLKTNIVWFNGKITQNFIDNILNSTTND
                     SE"
     gene            complement(1348..1836)
                     /locus_tag="PKH68_03410"
     CDS             complement(1348..1836)
                     /locus_tag="PKH68_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNX12018.1"
                     /translation="MKKLFMGLAVLIMFASAARAQSLDKFFNKFSDDERFGYVSVGSG
                     LMNLASAFSGVDREAKNMISKMKGVKILTLEEDTNSGLSNSFINELNDIVAKGDFETM
                     VEAREKNERTYIYKRIDAKNNADMLIITKEPKETTVIWLKGKITPEEMAKIMNDNNQN
                     GE"
     gene            complement(1936..2451)
                     /locus_tag="PKH68_03415"
     CDS             complement(1936..2451)
                     /locus_tag="PKH68_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12019.1"
                     /translation="MERIDELLKKYFEGTSTLAEEKELKTYFNGENIAENHVIYKSLF
                     CLFESERAEISPSITPITEDEKAKKINRKIILLTLSGIAACLLAITVIRTPSLSEDYL
                     IQHGKRINNPELAQQMARKKLEKSLAVVTRRLEPMQTLGKVETKMEKIEKIEKINQIK
                     QDIHNKLSKIK"
     gene            complement(2439..2945)
                     /locus_tag="PKH68_03420"
     CDS             complement(2439..2945)
                     /locus_tag="PKH68_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX12020.1"
                     /translation="MFNSLFTALSGKLYRLAKSMLRHTEEAEDALQEVQLKLWEKHDE
                     LDTVENPEGFAMRTMRNLCLDKIRKVHDTSELNEELISKNKNPYEQTELNDLTDRIKG
                     LIDRLPEMQRSIMRMRDVEEMEISEIAYIMNLTENAVTVNLSRARTKVKSQILNELQA
                     TNNTIWNE"
     gene            complement(3148..3921)
                     /locus_tag="PKH68_03425"
     CDS             complement(3148..3921)
                     /locus_tag="PKH68_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNX12021.1"
                     /translation="MFTIITLPLIPLRAEADERSEMISQLLFGQQVEILETTDSWYLV
                     KNLTDNCTGWIGKRTLSPRYFTAAPSDTSAFRAAKTPLVVCFRTSSVEKITLPGGSML
                     PPVQREQFELQDEIYQLAQFEPVFTKNNNGQLVIELANQYVNAPNLRGGKSIFGIDSS
                     GLVQIVFSMIGVLLPRYASQQVEFGTVVDFLSEVQAGDLAFFENEEGNIVSTGILINS
                     HQIIHSSGHVKIEIIDSQGIISSQTGDYTHKLRVIKRIL"
     gene            4232..6289
                     /locus_tag="PKH68_03430"
     CDS             4232..6289
                     /locus_tag="PKH68_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX12022.1"
                     /translation="MKFKLTQDTIKNVTKLGLFIALVAIIVQLFPKDKQFKYQYEIGK
                     PWSYELMTASYDFPIYKTEKQLDADKKELMENYSPYFQVDENVATQQLNRWRSDWKLK
                     NNTDPKYFAFVEKKLKTIYENGIISSLMYDRMKSKGYKSIVVVKPSRLTENVSIDELH
                     TPKTAYEEILMDKPYFISDAELSNYNLNLYLVENLRYDSITSEAVKKDMFKNLSLTSG
                     MVQTGERIIDRGEIVTPGTYAILNSMKIESEKRKSVFEESYLVLIGEIIIIFMLILLL
                     TFYLYLFRPYIFGTSKNLVFIALMILLIVGLSSLVLRFSSLSIYIVPFALLPIIIRVF
                     FDSRTALFAHIITVLIISFMVDNPFLFIILQITAGMTAVTGLKDMTQRSQLTQTALYI
                     FLSYTVMYLSSEFIAEGDIARIHFLPIINFALSSLLLLFAYVLIYILEKIFGLISAIT
                     LVELTNINSDLMMKFAEQAPGTFQHSLQVSNLATEAAKKINANSLLVRTGALYHDIGK
                     MKNPQYFIENQEGGKNPLLEMSYEDAAKAVVSHIADGVAIAKKYRLPDQIVGFITTHQ
                     GRTKTKYFYNSYVNANPGITPDENVFMYPGPIPFSKETAILMMADAVEARSRTLGEYT
                     EKSISEMVENMIDSQIADGQLKDAPISFRDVETVKKVFTEKIKNIYHNRITYPELQNQ
                     PKK"
     gene            6429..7721
                     /locus_tag="PKH68_03435"
     CDS             6429..7721
                     /locus_tag="PKH68_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX12023.1"
                     /translation="MKTDNTTYKLKAEIKPRKADQHHVLQLACEPLPIVRIALIGLGT
                     RGSLAVNRLMNIEGAQIAALCDIKDENLRNAQNHLKKHHQTPAAEYIVADDWRRACEN
                     PDIDLIYICTDWLSHVPIAVYAMEHGKHVAIEVPAALTVDECWQLVDTAERTRRHCMM
                     LENCCYDFFELNTLNMIRQGLFGEVMHAEGAYLHDLRSLNFPPREDTRKGMNRQKFSR
                     EHTGNLYPTHGLGPVCMAMKIHRDDKMNYLVSMSTKQQGITDYAGKVYGHNSPEVRQT
                     YKLGDMNTTLIRTENEKTIMIQHNISNPRPYSRIHSVTGTRGYFEKYPLPKIAIEPDT
                     NNFLTKKALNVLMKKYEHPFIKQYGKQAKKVSGSRALDFIMDSRLIYCLRNGLPLDMD
                     VYDAAEWSCLTELTELSVTNGSCPVEIPDFTRGDWKKY"
     gene            complement(7839..9899)
                     /gene="metG"
                     /locus_tag="PKH68_03440"
     CDS             complement(7839..9899)
                     /gene="metG"
                     /locus_tag="PKH68_03440"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNX12024.1"
                     /translation="MKMTFKRTTVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKG
                     EEVIFIGGSDEHGVPITIKARKEGVSPQDIVDRYHAIIKKSFEDFGITFDIYSRTTSK
                     THHQLASDFFKKLYEEGKFIEQTTEQYYDTEAQQFLADRYITGTCPHCGNENAYGDQC
                     EACGTSLSATDLINPKSAISGSVPEMRETKHWFLPLDQYEPWLREWILENHKEWRPNV
                     YGQCKSWLDMGLQPRAVSRDLDWGIPVPVEGAEGKVLYVWFDAPIGYISNTKELCDAQ
                     PEKFGSWETWWKDPETRLIHFIGKDNIVFHCIVFPSMLKAEGSYILPENVPSNEFLNL
                     ESDKISTSRNWAVWLHEYLEDFPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNEL
                     VAIYGNFVNRALVLTQKYFEGKVPALGELNDYDKQTLTDFTNVKTQVEALLDTFHFRD
                     AQKEAMNLARIGNKYLAETEPWKLAKTDMNRVATILNISLQIAANLSIAFEPFLPFSS
                     EKLRRMLSMETFNWNELGKTDLLAEGLQLGTPELLFEKIEDEQVEAQVQKLQNTKEAN
                     ELANYQPKAVKETVSYDDFGKLDIRVGTVLECTKVPKADKLLQFRLDDGMGGRTILSG
                     IAQSYPNPEELVGTQVCFIANFEPKKLRGVMSEGMILSAENSDGKLVLIQPKDLVTNG
                     VSIS"
     gene            10066..11463
                     /locus_tag="PKH68_03445"
     CDS             10066..11463
                     /locus_tag="PKH68_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX12025.1"
                     /translation="MPDAKTNSKLFKTKFSSAFWVANSVELLERLAYYAVFIVITLYL
                     SNIWGFSDIEAGAISGVFSATLYLLPLFSGAYADKIGFRKAIIVAFLLLTIGYLGLGV
                     LPTLLESAELVSYGKTTTFTGLNESYLRWSIVPVMAFIVLGGSFIKSVISGTVARETT
                     SENRARGYSIFYMMVNIGAFTGKTVVDPLRRSLGDQGLVYLNYFSASMTLMALIAVIM
                     FYKSAGKPAGEGKSFGQIGRDMIKVLSNGRLLALILIISGFWMIQSQMYATMPKYVIR
                     LIGDGATPGWYANVNPLVVFVLVNFITSFMKKRTALTSMIIGMLIIPFSALVMSFGNQ
                     LSADTILGMHPVAFMMIIGIAMQAVAECFISPRFLEYFSLQAPKGQEGLYLGFSHLHS
                     FFSYLFAFGLSGFLLDRYCPDPRGFSSPEAFAAATQHAHYIWYVFVGIGLISAFALLI
                     YGRLTVKELKKDTQE"
     gene            complement(11464..12390)
                     /locus_tag="PKH68_03450"
     CDS             complement(11464..12390)
                     /locus_tag="PKH68_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX12026.1"
                     /translation="MQLTKKHGHIALFGANLIFGLNTPLSRTIIPELIDPFSLTFLRL
                     AGSTALFWLLSLFLKKEKVPFKDIILLFFASIFGIVLNQVPYITGLSMTSPIDASLVT
                     SMLPIITMLLAALIIREPITWLKAIGVLVGASGAFILIMNSTNGNGKGNITGDLIILS
                     AALSFALYLTIFKKLISKYSPVTTMKWMFLFATIVSYPVLRKSIAHTDFMAMDLSVWL
                     RIGYIVVFATFITYLLIPIGQKTVRPTTLSMYNYMQPIVASFVAVGLGMDSFGYEKII
                     STLLVFAGVYIVTQSKSRAQMEQEKLRKQEQQ"
     gene            complement(12495..14741)
                     /locus_tag="PKH68_03455"
     CDS             complement(12495..14741)
                     /locus_tag="PKH68_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNX12027.1"
                     /translation="MTENNHTDHTAEDEIIQKEFDGLLQDYLNTNHRKKIEIITKAFN
                     FARAAHKGVKRRSGEPYILHPLAVARIVVSEIGLGSTSICAALLHDVVEDTDYTTEDI
                     ANLFGPKIAEIVDGLTKISGGVFGEKASEQAENFRKLILTMSEDIRVILIKIADRLHN
                     MRTLGSMPPAKQYKIGGETEYIYAPLAHRLGLFRIKTELENLSFKYEHPETYHEIEQK
                     LEGAKKSLNKFYNEFSKPIYHKLEEMGYEFELKERIKSVYSVWNKMSTRNIQFEDVYD
                     ILALRIIFKPKEGLNEKDQCWMLYSAITAIYKPHPERIRDWVSTPKANGYEALHVTVM
                     APGGRWVEIQIRSERMNEIAEKGLAAHWKYKTGDDEVSELDKWLKTIKELLENPEPNA
                     MDFIDTFKLNLFASEIFVFTPKGEIKTIAKDATALDFAFMLHSDVGYRSIAAKVNHKL
                     VPLNHKLNSGDQVEILTSRKQTPQSEWLQFVTTAHAKSKLRLYFKKEEKNLMTAGQKK
                     IEVALEDIQQKPGNENIVKLLNHYNLETRADLYLQAGQGQINLEDVSKIIFKTKSQSV
                     FSKILKNPFGSSSSADKKTPEVKPENDTKKFDFKKTYKLNDLDVGKSYELASCCHPIP
                     GDDVLGYVSEQKMIQVHKRQCSVAAIIKSNFGNRIVNVEWGTHNAFFAETLEIKGIDK
                     IGIVIEILKVISEENNVNVSKINIESNAGVFLGLFEVIIHDTKDINNLINNLIKIKEI
                     NSVQRVQK"
     gene            complement(14843..16045)
                     /locus_tag="PKH68_03460"
     CDS             complement(14843..16045)
                     /locus_tag="PKH68_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNX12028.1"
                     /translation="MTQQPKDNRRLFPEECLIREIAGKPRLSIGIPKENPGIETRLAL
                     TPEGVSIVTEEGHSVYVQRGAGNPMSYSDLQFSEAGAFMVDDMAEVYGADMVLKITPP
                     TLDELTMMHNRSTIFSMLQLSNMSKECIELIMQKKMNAIAYELIKDEQKAFPVVNSIS
                     EIEGNTAIAVISDLMSSERGGKGLLLGGVAGITPPEVLIIGASITGSVAARAAMALGA
                     QVKVFDHDINKLRKIQHYLGTQIFTSVIHPAVLFRALASADAVIGNLRYINGSERFMV
                     SEDLVKTMKKGAVIVDLSVDQGGCFETSECRTISNAVFVKHGITHYCVPNISARVGRT
                     SSMALSNIFSHILLKIGHSGSVRQSIAESSGFRHGAYIFNGIMVNRLIGDYYNIPSND
                     IGLLLAGY"
     gene            16209..16772
                     /locus_tag="PKH68_03465"
     CDS             16209..16772
                     /locus_tag="PKH68_03465"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNX12029.1"
                     /translation="MIDEIKKAIEILRNGGVILYPTDTIWGLGCDATNPAAVQKVYDL
                     KHRAEAKSMLILVDSPAKLQAYVEEIPDMAWDLIELTTKPLTIIYPGAKNLAPNLTGD
                     DRTIGIRVTSEEFSKRLCEMFRKPIVSTSANISGEKSPARFSEISDEIKNAVDYVVNY
                     RQNETTPAQVSSIIKLGTGNQIEIIRQ"
     gene            16779..>17950
                     /locus_tag="PKH68_03470"
     CDS             16779..>17950
                     /locus_tag="PKH68_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNX12030.1"
                     /translation="MNKNLLRIRYLIFDIVAALIVWVLFMIFRIVVNDGRAFQGLDIF
                     IPVYNFYSNLFLFPVLCLIIHYLSGYYENPVKESKSLEFFTTVIASAIISFIIFFVLL
                     LDDVVISYVNYYYSLMVLFLLLSSITLIIRVLQSSMIRRNFYKKKWTKNTLIIGTGAN
                     AQKMAEGIERNSYFNTIIGYISVDAAEKNVEPAKIVGNLQSIKDNIERKDVKEVIVAL
                     DHADEYKIFDIINKLFQYDIDIRFTPRQYEIVTGKARINHYGINPLVSVNKPTMNDWE
                     ISVKRTFDVIISMLAMILLAPLMLYLSLKIKQDSKGPVFYRQKRIGRNGAEFEILKFR
                     TMYYGSENGKPKLSSPNDERITNAGRLLRKYRLDEIPQFWNVLKGDMSIVGPRPER"
BASE COUNT         4893 a         3724 c         3814 g         5519 t
ORIGIN      
        1 cgatgatgct gctcaacggc gttttaagtt catgtgccac ctgttgggtc agtttacggc
       61 gcaccagtga gttttgttcc atctgctcgt gtcgcatctg gatagtaaat ctgtttgaga
      121 aataaaacag tatggcgata actataaagg taagcaccag ccaaaagtac aggaaaagat
      181 tatcgggaga aaggaccgat gagacgttca cattaaaagg tagcgatgag cgtacatagc
      241 agttatcgaa acgggtggcg tgataataat aaggcacacg gttggtttta gatgtccgta
      301 tgtccgaacc cgaacctttt tttcgggctt ccatcacctc gtttcgcgac agatgattat
      361 ccattttaga cacatcccga accacatcat catacaccac ttttcccgaa agatcgatta
      421 ttgttatccg ttcatctttg aaagtcgaat ttttgataag tgaatcgaga agattgagcg
      481 agcccccgga gtgttccaga aaataaaaaa tagcagcgtt ggtatcttca agtctcatat
      541 ccaacacatc agccttgtat tttttttctc tttggtattg aaaggcgagt accgaaatgg
      601 aaaagaccag aaaaatcggc agataataac ggataaacag ggttttggtg ttgtatgact
      661 tcatgttatt cctttgatga tagctaaaaa tgttaacaat ttaccggtgc aaagatagat
      721 tgtttttagg taatacatgt ttcaaaacta ttaaattcct attagtaagc aatttcagct
      781 ctcactccga atcatttgta gtcgaattta aaatattatc aataaaattc tgtgttattt
      841 ttccgttgaa ccacacaatg tttgtcttca aaccgttttt agttactatc attacatctg
      901 ctttgccccg tgatttcaac cgtttgtaaa tatacgtttt attggttttg ctgcgggtag
      961 agaatatggt atcgaatttt cccttatcaa taattttatg cagatcaccc gcaaatttcg
     1021 aggttaactt taagttggtg ctgtcttcgg tcaaagtcag tattttcaat ccgttcagta
     1081 tgggtagctc accgccggta tccaccgaaa ttccagatag tatggatgaa aagaactttc
     1141 cggtattcac attggtgtat tccacctgtt tgtttttggc gtatttacgg aaaaatctgt
     1201 tcagattttg tgcctgaccc atggcagtac acagcataat ggcgaaaagc agggctgtaa
     1261 gttttttcat gacaggtgta attgacagtg atacaatgtt tcgatgagaa gccggcatgc
     1321 actaatgccg gctttaatta gtggttttta ttctccgttt tgattgttgt cgttcattat
     1381 tttggccatt tcttcgggtg tgattttccc tttcaaccag atcaccgtgg tttcttttgg
     1441 ctctttggtg atgatgagca tatcggcatt gtttttagcg tctatccgct tgtaaatgta
     1501 ggtacgttca tttttttcgc gtgcttccac catggtttcg aaatctcctt ttgctacaat
     1561 atcattcagt tcattgataa atgaattgga gagccctgag ttggtatctt cttctagtgt
     1621 cagaattttt acacctttca ttttcgaaat catgtttttg gcttcacggt ctacgcccga
     1681 aaatgcggag gcgagattca tcaatccgct gcctaccgat acatacccaa accgttcgtc
     1741 gtcagaaaat ttgttgaaaa atttgtctaa tgactgagcg cgggcagccg aagcaaacat
     1801 aataaggacg gctaatccca taaataactt tttcatacga ttatttttta aaaatttttg
     1861 taatttgttt tttggtacga attcagcatt ggttttcccg ggtctgaata tcaagcaatg
     1921 ctggtttcgt gtctgttatt ttatttttga taatttgttg tgtatatcct gttttatttg
     1981 gttgattttc tctattttct ctattttctc cattttggtc tccactttac ccagggtttg
     2041 catgggttcc agcctgcggg taaccactgc cagtgatttt tccagttttt ttcgggccat
     2101 ttgttgagcc agctccggat tattgatccg ctttccatgc tgtatcaggt aatcctcaga
     2161 gagtgacgga gttctgatca ctgttatagc caagaggcac gctgcaatgc ctgaaagggt
     2221 gagcaggata atttttctat ttatcttttt tgccttctca tcttcagtta ttggtgtgat
     2281 ggaaggggaa atttcagctc tttcactctc aaacagacaa aataaagact tatatatcac
     2341 gtgattttcg gcgatatttt ctccgttgaa gtaagttttc agctcttttt cttcagccag
     2401 cgtactggtt ccctcaaagt attttttcag caactcatct attcgttcca tattgtgtta
     2461 tttgtagctt gtaattcgtt caaaatttgt gatttaactt ttgtgcgtgc acgtgagagg
     2521 ttaacggtaa ctgcgttttc ggtcaaattc ataatgtaag ctatttcact gatttccatc
     2581 tcctccacat cgcgcattcg catgatgctt cgctgcatct caggtagccg gtcgatgaga
     2641 ccttttattc tgtcagtcag gtcattgagt tccgtttgtt catacggatt tttgtttttg
     2701 ctgatcagtt cttcatttaa ttcgctcgta tcgtgcactt tgcggatttt gtccaggcag
     2761 aggtttcgca tggttcgcat tgcgaaaccc tccggatttt caaccgtatc cagttcgtcg
     2821 tgtttctccc aaagtttcaa ctgcacttcc tgcaaggcat cctcagcctc ttcggtatgg
     2881 cgaagcatcg attttgccag tcggtatagt ttgccggaaa gcgcagtgaa taatgagttg
     2941 aacattgaag catccataca ctataaatga cgaatggata gaaaaaatat tacattgaaa
     3001 ataaaaaaaa gaatttcttc ccaaaaaaac ggccttttat gccttcaaga gcctttttgg
     3061 ggatataaaa gaacacatgg agtaaaaatt cctgaagatt tttatcccat gcgttccctg
     3121 ttttttttgt tttaattcgt tttacagtta caaaattcgt tttatcaccc tcagtttatg
     3181 agtgtaatct ccggtttggg acgaaataat tccctgactg tcaattattt caatttttac
     3241 atgacccgaa gaatggatga tttgatgtga attgatgaga attccggttg acacgatgtt
     3301 accttcctca ttttcgaaaa aagccaggtc gccggcctgc acttccgaca gaaaatcgac
     3361 aactgttcca aattccacct gttgcgaagc gtagcgggga agcaatacgc caatcatcga
     3421 aaatacgatt tgcaccaaac ccgaactgtc aatgccgaaa atactttttc caccccggag
     3481 attgggggca tttacatatt gattggccag ttcaataacc agttgtccgt tgttgttttt
     3541 agtaaatacc ggctcaaact gagcaagttg gtatatctca tcctgcaatt cgaattgttc
     3601 gcgctgtacc ggtggcagca tacttcctcc gggcagcgta attttctcca cactcgaagt
     3661 cctgaaacat actaccagcg gcgttttggc agctctgaat gctgaagtgt ctgaaggtgc
     3721 agcagtaaaa taccgtggcg acagtgttct cttgcctatc cacccagtgc aattatcggt
     3781 cagatttttt accagatacc agctatcggt ggtttcgaga atttcgacct gctgcccgaa
     3841 aagcaattgc gagatcattt cgctgcgctc atcggcctcg gcgcgaagtg gaattagtgg
     3901 aagagtgatg attgtgaaca tatttttttt atttaaatga gggggtatat caagtcaaaa
     3961 aaagaataat ctgcctgtcg gagaacggta taaacatact tcttttctac aaaaataaat
     4021 aaagttcaaa cattttagac aagattcctg ttttttttat acttttatac ctttatagaa
     4081 tgctaaaaat ccgcgaagtg tcattccggt cgaataaacc tggatatttc tatagttaaa
     4141 ctttaacaca gaaaccaata ttactactgg ctgaaacaga aaatatgaaa ataatggaag
     4201 tttcacctga aaatacaaac agaataatcc gatgaaattc aagttaacac aggacacaat
     4261 caagaatgta acaaaattgg gcttgtttat cgctttggtg gccattattg tacagctgtt
     4321 ccccaaagat aagcaattta aataccagta cgaaatcggt aagccctggt cgtatgaact
     4381 gatgacagcc tcgtacgatt tcccgattta taagacagag aaacaactcg atgccgataa
     4441 aaaagaactg atggagaatt attctcccta ctttcaggtg gacgaaaatg tagcgacaca
     4501 acagttgaac cggtggcgct ccgactggaa attgaaaaac aataccgacc cgaaatattt
     4561 tgcctttgtg gaaaaaaaac tgaaaaccat ttacgaaaat ggcattatca gttcgttaat
     4621 gtatgacaga atgaaatcga agggttataa aagcatcgtg gtggttaagc ccagccgact
     4681 cactgagaat gtttctattg acgaactcca tacaccaaag actgcttacg aagaaatact
     4741 gatggataaa ccttacttta tcagtgatgc agaactcagc aactataacc taaacctcta
     4801 tctggttgaa aatctgaggt atgatagtat cacttctgaa gcagtaaaga aagatatgtt
     4861 caaaaatctc tccctcacat cgggcatggt gcaaacgggc gaacgaatta ttgaccgggg
     4921 cgaaatagta actcccggta catatgccat ccttaactcc atgaaaattg agtctgaaaa
     4981 aagaaaatct gttttcgagg aatcgtacct ggtgcttatt ggtgaaatca tcatcatttt
     5041 catgttaatt ctgctgttga ccttttatct ctacctgttt cgtccttaca tatttggaac
     5101 ttctaaaaat cttgttttca tcgccctgat gatattgttg attgttggat tgtcatcact
     5161 ggtacttcga ttttccagtt tgagcattta catcgttccc tttgcattac ttcccattat
     5221 aatccgggtg tttttcgatt cgcgtactgc actttttgct catattatta ccgtgctgat
     5281 catatcgttc atggttgaca atccttttct gtttatcatt ttacagatta ctgctggtat
     5341 gacagcagtg actggtctca aggatatgac gcagcgatct cagttgacac aaacggctct
     5401 ctatatattc ctgagttata cggtcatgta tctctcctct gagttcatcg ccgaaggtga
     5461 tatcgcccgc attcattttc ttccaataat caatttcgcc ctgagttcgc tgctactgct
     5521 gtttgcgtac gttcttattt atattttgga aaaaatattc gggctgattt cggctatcac
     5581 cctggtggaa ctgaccaaca ttaacagtga cctgatgatg aaatttgccg agcaggcacc
     5641 tggaaccttc cagcactcgc tacaggtttc caacctcgct accgaagcag ccaaaaaaat
     5701 taatgccaat agtttattgg ttcgtaccgg tgccttgtat cacgatatcg gaaaaatgaa
     5761 aaatccacaa tatttcattg aaaatcagga aggaggaaaa aatccgttac ttgagatgag
     5821 ttacgaagat gctgccaaag cggtagtcag ccatatagcc gatggcgttg ctattgccaa
     5881 aaaataccgg ctgcccgacc agattgtcgg atttatcacc acccatcagg ggcggacaaa
     5941 aacaaaatat ttttacaact cctatgtaaa tgcaaatccg ggtattacac ccgatgaaaa
     6001 tgtattcatg tatcccggtc ccataccttt cagcaaggaa acagccattc tgatgatggc
     6061 cgatgcggtg gaagcccgtt ccagaacgct gggagaatat acagagaaaa gtatcagcga
     6121 aatggtcgaa aacatgatcg attctcaaat agcagacgga cagctcaaag atgcgccaat
     6181 aagcttcaga gatgtggaaa cggtaaaaaa agtgtttact gaaaaaatta agaatatata
     6241 tcacaatcgt attacctatc cggaattgca aaatcaaccg aaaaagtagt gagatatcta
     6301 aattaagtca aaaacgaacc tttgcaacct gcatgaataa acacctgcag gttgtttttt
     6361 tttattactg gctatattta atttagcatc aggaaaaatt gaattatttt tgtgctgata
     6421 tagtgaatat gaaaacggat aatactactt ataaattaaa agcagagatt aaacctcgta
     6481 aggcagatca acaccacgtg ttgcaactcg cctgtgaacc gctgccaata gtgcggatcg
     6541 ctctcatcgg gttaggtacc cgcggaagtc tggctgtaaa tcgtttaatg aatattgaag
     6601 gagcacaaat tgcagccctg tgtgacatta aagatgaaaa tcttcgcaac gcacaaaatc
     6661 atctgaaaaa acaccatcaa actcccgccg ctgagtatat cgttgcagat gactggcgcc
     6721 gggcatgtga aaatcccgat attgacctga tttatatttg taccgactgg ctctcgcatg
     6781 tgccgattgc tgtgtatgcc atggaacacg gtaaacatgt ggcgatagaa gttccggctg
     6841 ccctcacggt agatgaatgc tggcaactgg tggatacggc cgaacgtacc cgtcggcact
     6901 gtatgatgct cgagaattgc tgttatgatt tttttgaact gaatacgctc aatatgatca
     6961 ggcaggggtt gttcggagaa gtgatgcacg ctgaaggtgc ctatctgcat gacctcagaa
     7021 gcctgaattt tccaccccgt gaagataccc ggaaaggaat gaaccgacag aaattcagcc
     7081 gtgagcacac cggaaacctg tatccaacac atggattggg gcctgtttgt atggcaatga
     7141 aaatacaccg cgatgataaa atgaattacc ttgtttccat gtccaccaaa cagcagggca
     7201 taaccgatta tgccggtaaa gtgtatggac ataattcacc cgaagtcaga caaacataca
     7261 agctgggcga tatgaatact acccttattc gaaccgagaa tgagaaaacg attatgattc
     7321 agcataatat cagcaatccg cgtccctaca gccgtattca ttcagttaca ggaacacggg
     7381 gatatttcga aaaatatccc ttgcccaaaa ttgctattga gccggatacc aataattttc
     7441 tgactaaaaa agcattgaac gtgttgatga aaaaatatga acatccgttt attaagcaat
     7501 atggaaaaca agctaaaaaa gtgagcggtt cccgggcact cgattttatc atggattccc
     7561 gattgattta ttgtctgaga aacggattgc cgctggatat ggatgtgtat gacgctgctg
     7621 aatggtcgtg cctgactgaa ctgaccgaat tatctgtcac taacgggagt tgtccggttg
     7681 aaatacccga cttcacccgg ggtgactgga aaaaatacta aaccacaact tgatctaaag
     7741 tctattttaa tggaaaaaaa agattgcgca ctgtctaact gacaattcgc aatctttttt
     7801 ttgaaagatg taatgtgatt aagatacatt gaacggcatc agcttatgct tacgccattg
     7861 gttacaaggt ctttgggctg aatcagaacc agttttccat ccgaattttc ggctgaaagt
     7921 atcatgccct cactcatcac accgcgtaat ttttttggtt cgaagttggc gatgaagcaa
     7981 acctgtgtgc cgaccagctc ttcggggttg gggtaggact gtgcaatacc cgacaggatg
     8041 gtgcgtccac ccatgccgtc atccagccgg aattggagca gtttgtcggc tttcggcact
     8101 ttggtgcatt ccagcacggt tcccacgcgg atatccagtt tcccaaaatc atcgtacgaa
     8161 acggtttctt tcacggcttt tggctgataa ttggccaact cattcgcttc ttttgtgttt
     8221 tgaagttttt gaacctgtgc ttccacttgt tcgtcttcaa ttttttcgaa caataattcc
     8281 ggtgtgccaa gctgcaatcc ttctgccagt aagtctgttt tacccaattc attccagttg
     8341 aaggtttcca tggataacat ccggcggagc ttttccgatg agaacggtaa aaacggttcg
     8401 aaagcaatgg aaagattggc agcaatctgc aaactgatgt tcagaatggt ggctacacgg
     8461 ttcatgtccg tttttgccag tttccacggt tcggtttcag ccaggtattt gttgccgatg
     8521 cgggccaggt tcatggcttc tttttgtgcg tcgcggaagt ggaacgtgtc gagcaatgct
     8581 tctacctgag tttttacatt tgtaaaatca gtcagtgttt gtttgtcata atcgttcagt
     8641 tcgcccaacg caggcacttt tccttcgaaa tatttctgtg taagcaccag tgcgcgattg
     8701 acaaaattac cgtaaatggc aaccagctca ttgttgttgc gtgcctgaaa atctttccag
     8761 gtaaaatcat tgtctttggt ttcgggagca ttggcagtga gcacatagcg cagcacgtcc
     8821 tgttttcccg ggaaatcttc caggtattcg tgaagccaca ctgcccagtt gcgcgaggtg
     8881 gatattttgt cgctttcaag gttcaaaaac tcattcgaag gtacattttc aggcaaaata
     8941 tagctacctt ctgctttcaa catacttggg aatacgatgc aatgaaacac aatattatct
     9001 tttccaataa aatgtatcaa acgggtttcc ggatctttcc accaggtttc ccagctgccg
     9061 aacttttcgg gttgtgcgtc gcacagttcc ttggtgtttg aaatatatcc aataggagcg
     9121 tcaaaccaca cgtaaagcac ttttccttcg gcaccttcca ccggcacggg aattccccag
     9181 tcgaggtcgc ggcttacggc acggggttgc aatcccatat cgagccagct tttacactga
     9241 ccatacacat tcggacgcca ttctttatga ttttcgagta tccattcacg cagccagggt
     9301 tcatattggt cgagagggag aaaccagtgt ttggtttcac gcatttcggg cacgcttccg
     9361 ctgatggctg atttcggatt gatcaggtcg gtggcactca gcgatgttcc gcaagcttca
     9421 cattggtctc cataagcgtt ttcgttgcca caatgcggac aagttccggt gatataacgg
     9481 tcggccagaa attgctgcgc ttcggtatca tagtattgct cagtggtttg ctcaatgaat
     9541 ttaccttctt cgtacagctt cttgaaaaaa tcggaagcca gctgatgatg agttttagaa
     9601 gtggtacggg aatatatatc aaacgttatt ccaaaatctt cgaatgattt tttgataatg
     9661 gcatggtaac ggtcaacaat atcctgcggg ctaacccctt ccttgcgggc tttgatggtg
     9721 attggcacgc catgctcatc cgaaccgccg ataaatatca cctcttcgcc tttgaggcgc
     9781 aggtagcgaa catagatatc ggccggaaca tatacaccgg caaggtgtcc gatgtgcact
     9841 ggaccattgg cgtagggcag ggcagaagtt acagttgtac gtttgaaagt cattttaatg
     9901 cgtttgtttt gttgaatatg ttgttttttt tggactgcaa agatacagag aatttattaa
     9961 aaattaataa gaaaactgct aattcaataa gacggtacag ggtgcttttt ccagcaaatt
    10021 tgttttgttt aactttgcat ctttacaaca tttaaaatcg ttattatgcc agacgctaaa
    10081 acaaattcta aattattcaa aacaaaattt tcaagtgcct tttgggttgc caattcagtg
    10141 gaactgctcg agcgcctggc atattatgct gttttcatag ttataacact ttatctttcc
    10201 aatatatggg gtttctctga tattgaagcg ggcgctattt cgggtgtatt ctctgcaact
    10261 ttatacttat taccactttt ttcaggtgcg tatgccgata aaatcggttt tcgaaaagca
    10321 atcattgtag ctttcttatt gttgacaatc ggttatttag gattaggtgt tttaccaacc
    10381 ctgcttgaaa gtgcagaact ggtaagttat ggtaaaacaa caactttcac cgggttgaat
    10441 gaaagttact tacgctggtc gatagtgccg gtaatggcgt ttatcgttct gggaggttcg
    10501 ttcatcaaat ccgtaatttc aggtactgtg gcgcgtgaaa ccacttctga aaacagggca
    10561 cgcggatatt ccatttttta tatgatggtg aatatcggtg cctttacagg taaaaccgtt
    10621 gttgatccgc ttcgcagaag tcttggtgat cagggacttg tttatctcaa ttatttttcc
    10681 gcttccatga ccctgatggc tttgatagct gttatcatgt tttataaatc agccggtaag
    10741 cctgccggcg aaggaaaaag tttcgggcag attgggcgtg atatgattaa agtcctgtcg
    10801 aatggcagat tgcttgcact gatattaatt atcagtggat tttggatgat acagagccag
    10861 atgtacgcta caatgcccaa atatgtaatc cgtctgatag gcgacggagc cacgccgggc
    10921 tggtatgcca atgtaaatcc gttggtggtg tttgtgctgg tgaattttat tacttcattt
    10981 atgaaaaagc gaacagccct cacgtccatg atcattggca tgctcatcat cccgttttcg
    11041 gctttggtga tgtcgtttgg taaccagttg tctgccgata ccattctcgg tatgcatccg
    11101 gtggctttta tgatgataat tggtatcgcc atgcaagctg ttgccgaatg ttttatttct
    11161 ccccgattcc tggaatattt ctccttgcag gctccgaaag ggcaggaagg cttatatctt
    11221 ggtttcagtc atcttcattc gtttttttcc tatctttttg cttttggact ttcgggtttc
    11281 ttacttgaca gatattgtcc cgacccgcgt ggtttcagct ctcctgaggc atttgccgca
    11341 gctacccaac atgctcacta catctggtat gtttttgtgg gaattgggtt gatttcagcc
    11401 ttcgctttgc ttatttacgg acgattaacc gtgaaagaac ttaaaaagga tactcaggaa
    11461 taatcattgc tgctcctgtt ttctaagttt ttcctgttcc atttgtgctc tggacttact
    11521 ctgggttaca atgtaaactc ctgcaaaaac aagcagggtg gaaataattt tttcatagcc
    11581 aaaactgtcc atgcccagtc ccacagccac gaacgaagcc acgatcggtt gcatataatt
    11641 atacatactc agcgtggttg gccgtaccgt tttttgcccg atgggtatta aaagataagt
    11701 aataaatgtg gcaaatacaa cgatgtagcc tattctcagc catacactca ggtccatggc
    11761 cataaaatcg gtatgagcaa tcgattttcg caaaaccgga taggagacga tggtagcaaa
    11821 cagaaacatc cacttcatgg tcgttacggg cgaatacttc gaaataagtt ttttgaaaat
    11881 agtaagatac agtgcaaatg acaaggctgc tgagagaata atcaaatcgc cggtaatatt
    11941 acccttgccg ttgccgtttg tgctgttcat tatcagaata aatgcacccg aagcgcctac
    12001 cagcacaccg atggctttca gccaggtgat gggctctctg atgataagtg ccgccagcag
    12061 catggtgatg atgggcaaca tgctggtaac cagcgaagca tcaatgggcg aagtcattga
    12121 caatccggtg atgtacggaa cttgattgag aacaatgccg aaaatggatg caaaaaacag
    12181 cagaataatg tctttgaatg gcaccttttc ttttttcaaa aataaggaaa gcagccagaa
    12241 taaggcagtt gaaccagcta accggagaaa agtgagcgaa aacggatcga tcagttcagg
    12301 aatgatggtg cgtgaaaggg gtgtgttgag accgaatatc aggttagccc cgaacagggc
    12361 gatatgaccg tgtttttttg tgagttgcat acgtaatttt gcagtggcag gttaaaatcc
    12421 tgcctggttt tttttgaaaa aaatcagggt gcaaaattac tcaaattcgg tgagaacggt
    12481 tacgggtggc gaagttattt ctgaacacgt tgtaccgaat tgatttcctt tatttttatc
    12541 aggttgttta tcaggttatt gatgtctttt gtgtcgtgga taattacctc aaataatcct
    12601 aaaaaaactc cggcattact ttcgatattg attttactga cgttcacgtt gttttcctct
    12661 gaaataactt ttagaatttc aattacaata ccaatcttgt cgatgccttt aatctcgagg
    12721 gtctcagcaa aaaaagcatt gtgagtgccc cattccacat ttacaatcct gtttccaaaa
    12781 ttgcttttta tgattgccgc caccgagcat tgccttttat gaacctgtat cattttttgt
    12841 tcgcttacgt atccaagcac atcgtcgccg ggaatgggat ggcagcacga agcaagctca
    12901 tacgactttc caacgtccaa atcgttcaat ttgtatgttt ttttgaaatc gaattttttc
    12961 gtgtcatttt ccggtttgac ttcgggcgtt tttttatcag ccgatgagct gctcccaaat
    13021 ggatttttca gaatttttga aaatacactt tgggatttgg ttttgaaaat aatttttgat
    13081 acatcctcca gatttatctg tccctgtccg gcttgcaggt acagatcggc ccgtgtttcc
    13141 aggttgtagt gattcagtag tttcactata ttttcattcc ccggtttctg ctgaatgtct
    13201 tccagtgcaa cttcaatttt tttctgaccg gcggtcatca ggtttttttc ttcttttttg
    13261 aaatacagcc tgagttttga tttggcatgc gctgtggtca caaattgcag ccattcgctt
    13321 tgtggcgttt gctttcgcga agtaagaatt tcgacctggt caccactatt gagtttgtga
    13381 ttaagaggaa caagtttatg attaaccttg gcagcaatgc tccggtaacc cacatcggag
    13441 tggagcataa aggcaaaatc cagtgctgta gcatcttttg cgatggtttt tatttcgcct
    13501 ttgggagtaa atacaaaaat ttcggaagca aaaagattta gcttgaacgt atcgatgaaa
    13561 tccatggcgt ttggttccgg attttcgagc aattctttga tcgttttcag ccatttatcg
    13621 agttccgata cctcgtcgtc gcctgttttg tacttccagt gggctgccag tcctttttca
    13681 gctatttcgt tcatccgctc gctgcgaatt tggatttcaa cccatcttcc acccggcgcc
    13741 attactgtta catgcagtgc ttcatacccg ttggctttgg gagtacttac ccagtcgcgg
    13801 atacgctcgg ggtgaggttt atagatggcc gtaatggcag aataaagcat ccagcactga
    13861 tctttctcat tgagaccttc cttgggcttg aaaataatgc gaagtgccag aatatcatat
    13921 acatcctcaa actgtatgtt gcgggtggac atcttattcc agaccgaata gacggattta
    13981 atcctttcct tgagttcgaa ttcatagccc atttcttcga gtttatggta tataggcttt
    14041 gaaaactcat tatagaattt attcagtgat tttttggcac cttcaagttt ttgctctatt
    14101 tcgtggtatg tttcggggtg ttcgtatttg aaactgagat tttcgagttc ggttttaatt
    14161 ctgaaaagtc ccaaccggtg ggcaaggggt gcgtaaatat attctgtttc acctccgatt
    14221 ttgtattgtt tggcaggtgg cattgagccc aacgtacgca tgttgtgcag acggtcggcg
    14281 atcttgatta aaatcacccg gatgtcttcc gacatggtca gaatgagttt gcgaaaattt
    14341 tcagcttgct cggatgcttt ctcgccaaat actccgccgg aaattttggt caaaccatcg
    14401 acaatctcag ctattttagg gccgaacaga ttggcaatgt cttcggtggt ataatcggta
    14461 tcttctacca cgtcgtgcag caaagccgca caaatagagg tggacccaag tccgatttcg
    14521 ctcaccacaa tacgtgccac agccagcgga tgaagtatgt acggttcgcc cgatcggcgc
    14581 ttcactccct tgtgagccgc acgtgcaaag ttaaatgctt tggtgattat ttcgattttc
    14641 ttccggtggt tggtgttcag ataatcctgc aacaacccat cgaattcttt ctgaataatc
    14701 tcatcttcag cggtatgatc tgtatgattg ttttcggtca tagtactttt ttgctgtgaa
    14761 ttctgtgcga caagattttc ctccactttt tcctcttttt tcacaggaat taaatcattc
    14821 atattttttt ctgaaaaacc acttaataac cggctaaaag gagcccgata tcattcgaag
    14881 gtatgttgta ataatcacca atcaagcggt tgaccattat tccgttgaat atataagccc
    14941 cgtggcggaa gcccgaactc tcagcaatag attgccgtac gctccccgaa tgaccaattt
    15001 taagcagaat gtgactgaaa atattgctga gcgccatgga tgaagttctg cctacacggg
    15061 ccgatatgtt gggcacacag tagtgtgtaa tgccgtgttt cacgaaaact gcattggata
    15121 tggttcggca ttccgatgtt tcgaaacatc caccctgatc gacacttaaa tcgacaatga
    15181 cggcaccttt tttcattgtt ttcaccaaat cttccgacac cataaatcgc tctgaaccat
    15241 ttatatacct gaggtttccg attacagcgt cagccgaagc cagtgctctg aacagtacag
    15301 ccggatgtat caccgatgta aagatctgtg tacccaggta atgctgaatt tttcggagtt
    15361 tatttatatc gtggtcaaaa accttaacct gcgctcccag cgccatggct gcacgggcag
    15421 ctaccgatcc ggtaatactt gccccgataa tcaacacctc gggaggggta attccggcta
    15481 caccacccaa caagagacct ttgccgccgc gttcgctgct catcagatcc gaaattacgg
    15541 cgatagcggt atttccttcg atttcagaaa tggaatttac caccggaaaa gctttttgtt
    15601 catctttaat gagttcgtaa gcgatggcat tcatcttttt ctgcataatg agctcaatgc
    15661 attccttcga catattactc aactgcaaca tggaaaaaat ggtggaacga ttgtgcatca
    15721 ttgtcagctc atcgagggtg ggaggtgtga ttttcaatac catgtcagca ccgtacactt
    15781 cagccatatc atcgaccata aatgcgcctg cttcgctgaa ctgaaggtct gaatatgaca
    15841 tcggattgcc tgctccccgt tgtacatata cactatgacc ttcctcggta acgatggaga
    15901 caccttcggg agtgagagcc aggcgtgtct caatgccggg attttccttc ggaataccta
    15961 tcgaaagacg gggttttccg gctatttctc tgatcaggca ttcttcagga aataatctgc
    16021 ggttatcttt tggttgttgt gtcatagttt gattttttaa tagccacaaa tctaattact
    16081 attcttgaat tgaacaaatt gtgtgaggaa taatttactg ccgcttgccg atattttaat
    16141 tatctttgta accttgttaa tgaaataaaa ccgaatgttt ttcgttatct tttaattgca
    16201 taaggtctat gattgatgaa ataaaaaaag cgatagaaat tctgcgaaac gggggtgtga
    16261 ttttgtatcc caccgataca atctggggcc tggggtgcga tgccacaaat cccgcggctg
    16321 tgcagaaagt gtatgacttg aagcaccgtg ccgaagcaaa atcgatgctc atactggtgg
    16381 attcgccggc aaaattgcag gcatatgtgg aagaaatacc cgatatggcc tgggatctga
    16441 tagaattaac caccaaaccg cttaccatca tatatcccgg tgccaaaaac ctggctccca
    16501 atttaaccgg ggatgaccga actataggaa taagggttac atcggaagaa ttttcgaaac
    16561 gactgtgtga aatgtttcgc aaacctattg tatcaacttc tgccaatatc agcggggaaa
    16621 aatcgcctgc ccggttttcg gaaatttcgg atgaaataaa aaatgcggtg gattacgtgg
    16681 tgaattaccg acaaaatgaa accacaccgg ctcaggtaag ttcaattata aaactcggta
    16741 caggaaacca gattgagatt atccgtcagt aaacgaccat gaataaaaat ttactcagaa
    16801 taagatacct gattttcgat attgtggcag cgctcattgt atgggtgctt tttatgatat
    16861 ttcgcatcgt ggtgaatgat gggagggcat ttcagggact cgatatcttt attccggttt
    16921 ataattttta ttccaatctt ttcctttttc ctgttttgtg tttgataata cattatttgt
    16981 caggatatta cgaaaatccg gtaaaagaat caaagtcact ggaatttttc accacagtca
    17041 ttgcctcagc cattatctca tttatcatat tttttgtgtt gctgctcgat gatgtcgtca
    17101 tcagttacgt caattattac tactccctga tggtgctgtt tttgcttctt tcgtccataa
    17161 cgttgattat ccgtgtgttg caaagttcga tgatccgacg aaatttttac aaaaaaaaat
    17221 ggacaaaaaa cacgctgata atcggtaccg gtgcaaatgc ccaaaaaatg gccgaaggaa
    17281 ttgaaagaaa ttcctatttt aacaccataa taggttatat ttcagttgat gcggccgaga
    17341 aaaacgtaga gcctgcaaaa attgttggca acctgcagag tattaaagat aatattgaac
    17401 gaaaggatgt aaaggaagtg attgtagcgc tcgaccacgc cgatgaatat aaaatttttg
    17461 atatcatcaa caaattattt caatacgata ttgacatccg gtttacaccc cgtcagtatg
    17521 aaatcgtaac cggtaaagcc agaattaatc attacggcat caatccgctg gtaagtgtaa
    17581 acaagccgac tatgaacgac tgggagataa gtgtcaaaag aacttttgat gtgataattt
    17641 cgatgctggc aatgattttg cttgctcccc ttatgcttta tcttagcctg aaaataaaac
    17701 aggactcgaa aggtccggtt ttttatcggc aaaaaagaat tggcagaaat ggagcagaat
    17761 ttgaaatact taagttcaga accatgtact acggctccga aaacggtaaa cccaaactca
    17821 gcagcccgaa tgatgaacgg attacgaatg ccggccgatt gttgagaaaa taccgactgg
    17881 atgaaattcc acagttttgg aatgtgctga aaggggatat gagcatagta ggacctcgtc
    17941 ccgaaaggaa
//
LOCUS       DAOPZK010000034        17765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3341_length_17765_cov_24.137775, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000034 DAOPZK010000000
VERSION     DAOPZK010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3341_length_17765_cov_24.137775"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..680)
                     /locus_tag="PKH68_03475"
     CDS             complement(60..680)
                     /locus_tag="PKH68_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNX12031.1"
                     /translation="MTETDNNAGKSGKARPAADDEKAIRKQTIERAKRIQAARKAEAA
                     KKAADEKKALKLKKEEAKKPVVGSAKKTANVSVNPVPEKTEEKIIKQEKAMIAEEKKE
                     PEFVDFAITARKYKTLLTNKYKNRKFWVNPSPYEIQSYIPGTIIEVFVKEGRVVKEGD
                     PLLILESMKMQNRIDMPFTAKIKKINVKAGERIPKDFLMIELMPEK"
     gene            complement(759..2303)
                     /locus_tag="PKH68_03480"
     CDS             complement(759..2303)
                     /locus_tag="PKH68_03480"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNX12032.1"
                     /translation="MSLKKNIIALRQKKAIVEKGGGDKALEKQIAMGKMPARVRILAV
                     LDEGSFHEYDMFVEHEEKNFGMANKELPAEGVITGTGTINGAPVCIYAQDFTVMGGSL
                     GLKHARKITKIMDHALKMKIPCIGINDSGGARIQEGIGALAGYGEIFFRNTMASGVIP
                     QLSVILGPCAGGAVYSPALTDFVFVVENISKMFITGPNVIETVLGEKISQEDLGGARV
                     HAEITGNAHFYATSEMECFKQIKTLISLIPHNNEDKAKSVPPKEPKFRKKIENIIPAD
                     PKQPYDVRDVILALADESKFFEVQELWAANIVIGFGRMNGETVGFVANQPMYLAGVLD
                     CDASDKAARFIRFCDSFNIPIITLEDMPGYLPGVDQEHAGVIRHGAKMLYAYSEATVP
                     TITIILRKAYGGGYIAMNSRHLGADFVYAWPSAEIAVMGPEGAANIIFRKEIMEAEDE
                     DAMRQQKVKEYVDKFANPFVAASKGYIDAVIEPAETRDMLVHSLEVSKRKEEHRPFKK
                     HGIPPF"
     gene            complement(2396..2929)
                     /locus_tag="PKH68_03485"
     CDS             complement(2396..2929)
                     /locus_tag="PKH68_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12033.1"
                     /translation="MNFNEVIAHRLTMKEVHLVRDELRQNKEHFADFFQLIFQDPYKI
                     AWQAAWICEKVSEVDPSMFTDEDYRQLIDFTLTNKEDSLQRLCLSVLFNLPFRKPLSV
                     EFINNCFEQMISPKKPVGVQVLSMKILHKICLVEPGFTDELLLSLENLDYFNYSAGFV
                     AARNQVIKSLIKSMIRY"
     gene            complement(2957..3247)
                     /locus_tag="PKH68_03490"
     CDS             complement(2957..3247)
                     /locus_tag="PKH68_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNX12034.1"
                     /translation="MRRRNTEKLSDILGQVLKQNHLDEKLYETRVLKSWSVVLGENVM
                     NYTSNLYFSKKRLYVTLTSSVLRQELFLTREEIRNSLNNYVGFPVVKEIVFQ"
     gene            complement(3252..4190)
                     /locus_tag="PKH68_03495"
     CDS             complement(3252..4190)
                     /locus_tag="PKH68_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNX12035.1"
                     /translation="MNKPLIIVIALILTLPATFAQNRSASYLTYIEKYSSLAVEQQKR
                     YRIPASITLAQGLLESGAGRSELSLSSNNHFGIKCHNTWEGEKVYHRDDHADPECFRK
                     YNEVRDSYEDHSLFLTKRNHYSFLFDLNPTDYKGWAYGLKKAGYATDPAYPVKLIKII
                     DDYELHRFDLEDKSEYLASKTDYPKKRHTPIVTNSSTTLYQHELFRNNKVKCIFSLPG
                     DTYAGIAQEFNLSLNKLLQYNDLAQTQELEPGTIIYIKEKKKKAARGYLQHVVKEGEN
                     MYRISQKYAIKLDKLYDLNKMSYSSGAEVGLKLKLR"
     gene            4318..4800
                     /locus_tag="PKH68_03500"
     CDS             4318..4800
                     /locus_tag="PKH68_03500"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNX12036.1"
                     /translation="MEQITYETRIKVYQPTEIPAEFLPLIDEAKLQTQKSYALYSGFK
                     VGAAILLANGIVVGGNNQENSAYPSGLCAERTAMFYANSQYPDTAVLTLAIAAFTNGE
                     FTSEPIAPCGACRQVLLETENRYDKPIKIILYGTDKIYVLENVKQLLPVSFGKDSLNR
                     "
     gene            4918..5301
                     /locus_tag="PKH68_03505"
     CDS             4918..5301
                     /locus_tag="PKH68_03505"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNX12037.1"
                     /translation="MKKEIFSIEYPLNNASEIVLWRMIGTPLGLAEWFSDGVTVEGNE
                     YTFTWKGHEQIAFLKQIKEGKLISFQWEEDKNTDAYFQFEIVTLDLSNHVGLVITDFT
                     TADDKEDAILLWNSQVETLCRIGGM"
     gene            complement(5312..6373)
                     /gene="mnmA"
                     /locus_tag="PKH68_03510"
     CDS             complement(5312..6373)
                     /gene="mnmA"
                     /locus_tag="PKH68_03510"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNX12038.1"
                     /translation="MNIATLISGGVDSSVVVHLLKESGITPTLFYIKIGMDDDELLHC
                     SSEEDIEMASLIARKYGCRLEVVDLHQDYWDSVVAYTIDKAKQGLTPNPDVMCNKLIK
                     FGVFEQRVGYNFDKTATGHYATTIEQNGKIYLGTAKDPVKDQTDFLAQINYLQVSKLM
                     FPVGNLMKSEVRDIALKAQLPSAKRQDSQGICFLGKVNYNEFIRRYLGVKTGPIVELE
                     TGKILGHHHGYWFHTVGQRKGLGLSGGPWYVIKKDVEANIVWASKGYDVETQYGYEFE
                     MKDFHFITENPWMNTAESADICFKIRHTPEFTQGKIHKTSSGYRIVSSEKLQGIAAGQ
                     FGVVYDRDAHICIGSGEIQ"
     gene            complement(6388..7038)
                     /locus_tag="PKH68_03515"
     CDS             complement(6388..7038)
                     /locus_tag="PKH68_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX12039.1"
                     /translation="MQQIILSDNQDISRAGLIHIFSAAGSYRFSEADNKKELIACLES
                     HPESLVVLDYTLFDIQSAEELLVLNAGFGKAGWLLFSNELSDEFVKQIYINSSNISIL
                     YKDSSRHELLMALHEIQKGRRYFSNIVSKSLLESAYKHQSEGTDAKLTATEKEILKDV
                     ASGRTTREISENRHVSVHTVVSHRKNIFRKIGVNNVHEATKYAVKAGLIDLADYFI"
     gene            complement(7141..8040)
                     /gene="gldN"
                     /locus_tag="PKH68_03520"
     CDS             complement(7141..8040)
                     /gene="gldN"
                     /locus_tag="PKH68_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNX12040.1"
                     /translation="MKKYSILLVVISLFVVSNKTNAQKTLQFFDEIGVEKLQTAEMDA
                     MADTIAVVNHRWDDIAWSRTVYRVIDMRDKQNFQLYFPTRPNDQYRSLFRVMLDAVAN
                     GINVYKRDPRDIKPSWKDVLTGYELSRVFAYDESTENNLLQVDTITEQRSVGVDQYAR
                     YIRNQLKFLIQEKIFFDKHTSRMYSKIMAIAPLYALHPDNMDSKESMAYFRNSVICWF
                     AFDELRPYLIRQFVVPKGNDTERMTFDDFFNQKMYSSYLLGESSTYGRMLLEYAVDED
                     ALRAEQKRIDTELINFEEDLWEY"
     gene            complement(8073..9596)
                     /gene="gldM"
                     /locus_tag="PKH68_03525"
     CDS             complement(8073..9596)
                     /gene="gldM"
                     /locus_tag="PKH68_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNX12041.1"
                     /translation="MSGAKNCPETPRQRMIGMMYLVLTAMLALNVSSEILEGFGLVDR
                     GLKNSIESAEIRNKGMYADFQEEAVKNPQKIGEWLKLARNVEAQSNDLYKYIGDYKYQ
                     ILKIADKDKADKNAVNIIARDNLDAAGQYSLVGGHYKELKKKIETYRELLIQLSEGDI
                     AKQQMYKKIFDTSNKFGKTWQSSMFESMPVAAVITMLTKYQADVRSAEAETVQYLRTR
                     ADLKDFRVNKLEAFVIPESSYILQGGTYTAKIVLAAIDTTSSYTYSVNGGRLSGNVYR
                     AGGSTPGTYKYSGSISVPNNQGDVKSYPFTSEYVVGKPSATLSNVDLNVVYKGIDNKF
                     SISAPGIPDENLRISADGATVGRSGNFFIIKPTRDGEIKINVSAVMQGKTVSMGSGIY
                     RVKYLPDPTAYFAYTDAGGVPRTTQDAQMSKKVLRSGRLIASYGPDELVKANFTITSF
                     TVHTIFGTATSSSSEFSPRQLSLMTELEGGDVVTLKNIKAVGPDGKVRSLGLIQIEI"
     gene            complement(9699..10592)
                     /gene="gldL"
                     /locus_tag="PKH68_03530"
     CDS             complement(9699..10592)
                     /gene="gldL"
                     /locus_tag="PKH68_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNX12042.1"
                     /translation="MAQEHSTFYRMWNSNRGKKIISAAYSLGASVVILGALFKILHLA
                     GANYMLMAGMITESVIFALGIFDTPHKDYDWGQIFDFKNPEKLNLMATGIQSPGSTAA
                     VAPARQVGLNYTEAISDDEVKKLSEGIKNLSATADNLQVLANVAVAANGLTKNIETAA
                     EATVNFAGTQQKLNTTSETLAAKYQGITSEMDVITNTTKSYADKVAEVNKSLASINSI
                     YEIQLRHIQSQTEGLNLQSENIRTVSGHLDDITSDILKMKEATALTLEDSLKYQVTTK
                     KIAKQVEDLNAVYGNMLNALS"
     gene            complement(10638..12020)
                     /locus_tag="PKH68_03535"
     CDS             complement(10638..12020)
                     /locus_tag="PKH68_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX12043.1"
                     /translation="MYKIRMKRFLPILFVASLFVSCNKSAGELVGVGNTGTFHEANPY
                     GMIFVKRGSFMMGANDQSAFSGANDRSMNVTVDAFWMDETEITNDEYKQFVNWVKDSI
                     ALRKLVIAGKDEYRKQFKNQSDDASPEEARLNWKAKIPWNSKDEDVREILSSMYYDGN
                     NLLGSRRQIDPTKLQYKYEWLNTDQAALPGNKFNVNTGAYYPGARARVDSSYVDENGV
                     IHNVTITKKLRSRRDLISNRIVNVYPDTISWLRDFQFAYNDPKMKMYFSHKGFSQYPV
                     VGVSWEQAQAFCQWRTNYFNNSNSVGGQDYRLPTEAEWEFAARGGRDLSLYPWGGNYV
                     RDAKGCFLANFKPMRGSYTDDTGAATMKVGSFAPNNFGLYDMAGNVSEWTSTAWGGSS
                     NMVVSDMNPNYQYNAKNDDPDVLKRKVVKGGSWKDIAYYLQCGVKTYEYQYESRPYIG
                     FRCVRSYNGE"
     gene            complement(12053..13033)
                     /locus_tag="PKH68_03540"
     CDS             complement(12053..13033)
                     /locus_tag="PKH68_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNX12044.1"
                     /translation="MTNPFENRKTKRIVLLIFSMACTLPAFSQFEAQLSQYMFHPATY
                     NPAAIAEGGMINVTGQHKIQWLGMPGAPQTTFFTINAPFRGSKTVTQGLGIRFLNDKI
                     GAFTNQSAGLQYSFKKNIGKGVLSMGADLGFVSVGFIADSVKKSDINSEYHDFSGDMT
                     IPTADENGMNLDMSVGAFYSAPKYYAGISYVHLNAPAVKLGEKSIFHVRGVMYLTGGY
                     DAAFANPKLLLRTSTLLKTDYTTWQAELSSRLEYDKRFWGGLSYRYQDAVIVLAGLNL
                     ASGFTLGYAYELPTGKMLTVSSGSHELLLSYSFAFDLSKNKNKYKSIRIL"
     gene            complement(13179..13400)
                     /locus_tag="PKH68_03545"
     CDS             complement(13179..13400)
                     /locus_tag="PKH68_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNX12045.1"
                     /translation="MYWTLELASKIEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGEIYETIEDIWPDYPSKEDFFFNEEEY"
     gene            complement(13476..14030)
                     /locus_tag="PKH68_03550"
     CDS             complement(13476..14030)
                     /locus_tag="PKH68_03550"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNX12046.1"
                     /translation="MTKLYTKTGDKGQTGLIGGTRVSKADIRLEAYGTVDELNAQIGL
                     LVAEDLNPEHTTFLQFIQNLLFTVGSNLATDTTKTGYRVASVMKHEYITAIEQEIDRL
                     DSQLTPLTNFILPGGSRKSALCHVCRTVSRRAERRIIEMSNVYETENKIIVFVNRLSD
                     YFFVLSRYMLMEDQMQEILWKQPK"
     gene            complement(14027..14701)
                     /locus_tag="PKH68_03555"
     CDS             complement(14027..14701)
                     /locus_tag="PKH68_03555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX12047.1"
                     /translation="MIYEKTPFYIFPEIEKLRASLRRDQRQLHLTDYGTGNRTVSKVC
                     DVARKSLKSPRQAQLLYRIVNFTKANTVLELGTSLGITTSYLAASNSQLRCITLEGSE
                     ELAAVARDNFRKLGLKNIEIVTGNIDQTLDAVLNGIETLDVVFFDANHRKEAVLQYFE
                     KCVKHISRYSVFIVDDIHWSRDMEDAWIEIQNHRDVQSTIDLFDCGLVFFDSYLSNKH
                     YKMKHL"
     gene            complement(15050..16699)
                     /locus_tag="PKH68_03560"
     CDS             complement(15050..16699)
                     /locus_tag="PKH68_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX12048.1"
                     /translation="MQLSDRTLSDWLEHWAAETPDKEYIVYSDRNLRFTWKTFNERVD
                     NMAKGLIAIGVSRGTNVGIWAQNVPDWLTFLYATAKIGAVAVTVNTNYQSEEVSYLLK
                     NSDMHTLCMTDGVAGNNYTDIIYELVPELKTTQRGKFKSERFPELKNVVYIGQEKYRG
                     MYNTAEILLLGNNVESDELTRLRNLTGCHDVVNMQYTSGTTGFPKGVMLTHHNIANNG
                     YLTGVHMKFTPEDKCCICVPLFHCFGVVLATMCCLTHGSTQVMVERFDPLITLASIHK
                     ERCTVLHGVPTMFIAELNHPMFSLFDMSSLRTGIMAGALCPIELMRQVNEKMFMDITS
                     VYGMTETSPGMTQTRVDDTFEVRCTTVGREYEFTEVKVLDPETGLECPVGVQGEICCR
                     GYNVMKGYYKNPEATAQVIDKNGFMHSGDLGVKDENGNYRITGRIKDMIIRGGENISP
                     KEVEDYLYKIPEVRDVQVVAVASPKYGEDVGAFIILKDGCELTPEDVRDFCKNHIARY
                     KIPRYVFFIKQFPMTGSGKIQKFRLREMALEMCQQQGIEIV"
     gene            complement(16912..17478)
                     /locus_tag="PKH68_03565"
     CDS             complement(16912..17478)
                     /locus_tag="PKH68_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNX12049.1"
                     /translation="MNTVTEKIKTLCADKNISVEELAEGTGLTVEQIQQILNSQIIPS
                     LSSLIKIARALGVRLGTFLDDSENFGPVVNRSADKQQAATFSSQLSTANSHMDFFSLA
                     SRKAARHMEPFLIDLKPSADGEKVSSSHEGEEFLYVLSGTVLVQYGREQYILEQGDSI
                     YYDSIVDHLVTTANDQPAQILGIVYSPF"
BASE COUNT         4656 a         3923 c         3664 g         5522 t
ORIGIN      
        1 aaatgcctgc agaattttcc tctgcaagca tttttttttc aatttgttat cctgaaaatt
       61 tatttttcgg gcattaactc tatcatcaga aaatctttgg gaatgcgctc gcccgctttt
      121 acatttatct tttttatttt agctgtaaag ggcatatcaa tgcggttttg cattttcatg
      181 gattcgagta tcagcagggg atcgccctcc ttaacaaccc ttccctcttt gacaaagact
      241 tcgataatag tacccggaat atacgattga atttcgtacg gacttggatt gacccaaaat
      301 ttccggtttt tatacttatt ggtcagtagt gtcttatatt ttcgggcagt aatggcaaaa
      361 tcaacaaatt cgggttcttt tttttcttct gctatcatag ctttttcctg tttaataatt
      421 ttttcttcgg ttttttcagg cacaggatta acactgacat tggctgtttt tttggcactg
      481 ccgacaaccg gtttttttgc ttcttccttc ttaagtttca gcgctttctt ttcatcagca
      541 gctttcttgg ctgcttcagc ttttcgcgct gcctgaatgc gtttggctcg ttctattgtt
      601 tgtttcctga tagctttttc atcatcagca gcaggtcggg ctttacctga ttttccggcg
      661 ttattatctg tttcagtcat acttttctct ttattaaaaa ataaatttaa cttactcgaa
      721 tcagcacctt tacatcacaa cgaatcgtaa cctctccctt aaaatggagg tattccgtgt
      781 tttttgaacg gacggtgctc ttcctttctt ttagatactt ccagcgagtg aactagcata
      841 tcgcgggttt cggcaggttc gattacagca tcgatatatc cttttgatgc agccacaaac
      901 ggattggcaa atttgtcaac atattccttt accttctgct gacgcatggc gtcttcgtct
      961 tcggcctcca tgatttcctt tcggaaaata atattggcag caccctcggg tcccattacg
     1021 gctatctcgg cacttggcca tgcatacaca aagtcggcac ccaggtgacg tgaattcata
     1081 gcaatgtaac cccctccata cgcttttcgc aggatgatgg ttattgtcgg tacagtggct
     1141 tcagaataag cataaagcat ttttgcaccg tgacggataa ctcctgcgtg ttcctggtct
     1201 acacccggta aataacccgg catatcttcc agcgttatga tagggatatt gaaagaatca
     1261 cagaagcgaa taaagcgggc agctttatcc gaagcatcgc aatcgagcac tccggccaga
     1321 tacatgggct gattggccac aaaccccacg gtttcaccat tcatgcgtcc aaaaccaata
     1381 actatattag cagcccaaag ttcctgcact tcaaagaatt ttgattcgtc ggcaagagcc
     1441 aggatgacat cacgtacatc gtagggttgc ttcggatctg caggtataat attttcaatt
     1501 ttctttctga atttgggttc tttaggtggc acgcttttgg ctttatcttc attattatga
     1561 ggaataagcg atatcaatgt tttgatttgt ttgaaacatt ccatttcact tgttgcgtag
     1621 aaatgcgcat ttccggttat ttcagcatgt acacgggcgc caccaagatc ttcctgcgag
     1681 attttctcac caagcaccgt ttctatcaca ttggggccgg tgataaacat tttggagata
     1741 ttttcgacta cgaaaacaaa atcggtcagt gcgggagagt aaacagcacc accggcgcag
     1801 ggaccaagga taaccgataa ctgtggaata actcccgaag ccatggtatt gcggaaaaat
     1861 atttcaccat atccggccag tgctccgata ccttcctgaa tacgtgcacc tcccgagtca
     1921 ttgataccaa tgcagggaat tttcattttc agcgcatgat ccattatttt agtgatttta
     1981 cgggcgtgct tcagtccaag tgagccaccc atcacagtaa agtcttgtgc ataaatacag
     2041 acaggagccc cgttaattgt tccggttccc gtgattacac cttcggcggg taattctttg
     2101 ttagccattc caaagttttt ttcttcatgt tccacgaaca tatcgtattc atgaaatgaa
     2161 ccttcgtcga gtacagctaa tattcgcaca cgggccggca ttttacccat agctatctgt
     2221 ttttcaaggg ctttgtcgcc accgcctttt tcaacaatag cttttttctg tcgtagagcg
     2281 ataatgtttt tctttaaaga catacttttt aatttaaata aaacaattta ttatcggacg
     2341 caaaggtaac actaaaatcg ctaaacagca tcatttttaa ctttctgttt ggacgttagt
     2401 agcgtatcat gctttttatc agcgacttaa tcacctggtt acgtgcagct acgaatcccg
     2461 cactgtagtt aaagtaatcc aggttttcga gactaagcag gagttcatct gtaaaaccgg
     2521 gttctacaag gcaaatctta tgcaaaatct tcatggagag tacctgcaca cccaccggtt
     2581 ttttgggcga tatcatctgc tcgaaacagt tattaataaa ctcaacagat aatggttttc
     2641 gaaagggtag gttaaataaa acagaaagac agaggcgctg taagctatcc tctttgttgg
     2701 tcagggtaaa atctatcaat tgacgatagt cttcgtcggt aaacatcgaa ggatccactt
     2761 cgcttacttt ctcacaaatc caggctgcct gccaggcaat tttataggga tcttgaaata
     2821 tcaactgaaa aaaatcagca aaatgctcct tgttctgtct cagttcatcg cgtaccagat
     2881 gaacttcttt catagtcagc cggtgagcta taacctcatt gaaattcatg gttgaaatta
     2941 agtttagcaa acgaaattac tgaaacacta tctcctttac aaccggaaaa ccgacataat
     3001 tgttcagtga atttcgaatt tcttcgcggg tgaggaaaag ttcctgcctc agcaccgacg
     3061 aagtgagggt gacatacaac cgttttttac tgaagtacaa atttgaggtg tagttcatca
     3121 cattctctcc caacacaacc gaccacgact tgagcaccct ggtttcgtag agtttctcat
     3181 cgaggtgatt ttgcttcaac acctgtccca gaatatcgct cagtttttcg gtatttcgtc
     3241 tgcgcatagg gctatcttaa tttcaatttc agtcccactt ctgcacctga tgaataggac
     3301 attttgttga gatcgtaaag tttatcaagc ttaatggcat atttctgcga aatccggtac
     3361 atgttttcgc cctctttaac tacgtgctgc aggtatccgc gtgccgcttt tttctttttt
     3421 tccttgatat aaataatggt tccgggctcc aactcctgag tttgggcaag gtcattgtat
     3481 tgaagcagtt tattcagcga caggttgaat tcctgtgcaa taccggcgta ggtgtcaccc
     3541 ggaagtgaaa agatgcattt cactttgtta ttccggaata gttcgtgttg gtataatgtt
     3601 gttgatgaat tagttacaat tggcgtatgt ctcttcttag gataatcagt ttttgaagcg
     3661 agatattccg atttatcctc cagatcaaac cggtgcaatt cgtaatcatc tattatttta
     3721 atgagtttca ccgggtatgc gggatctgtg gcataaccgg ctttcttcag tccgtaagcc
     3781 caccctttgt aatcggttgg atttaagtca aacaaaaaag aataatgatt ccgtttggtt
     3841 aaaaacaggg aatgatcctc gtacgaatct ctcacctcat tatacttcct gaaacactcc
     3901 ggatcggcgt gatcatcgcg atgatagacc ttctccccct cccaggtgtt gtgacatttg
     3961 atgccaaagt gattgttgga cgagagtgaa agttcacttc ttccagctcc agattccaat
     4021 aaaccctgag ccagggtgat gctggcagga attcggtatc gtttttgttg ctctactgcc
     4081 aggctggaat acttctcaat ataagtcagg tacgaagccg aacggttttg tgcaaatgtg
     4141 gccggaaggg tgagaatcag tgcaatgact atgatcaggg gtttattcat tatatagttg
     4201 tgaataatag attcggtttg cgataataat ttccacaaat ataaaactta ttgacgtaaa
     4261 aagcggtttt ggttaaatta ttttatatat ttgtaccatt acaccaattt ttgacatatg
     4321 gaacaaatta catacgaaac caggataaaa gtatatcaac caactgaaat tcctgcagaa
     4381 ttccttcctc tgattgatga ggcaaaatta cagacccaaa aatcatacgc gctttattcc
     4441 ggatttaagg ttggtgctgc tattttgctg gcaaacggca tcgttgtggg cggtaataat
     4501 caggaaaatt cggcatatcc atctggcttg tgtgccgaac gcactgccat gttttatgcc
     4561 aattcccaat atcctgacac agcggtactg acgctggcga tagctgcatt taccaacggc
     4621 gaattcacat cagaacccat tgctccctgc ggggcatgcc gtcaggtact gctggaaacc
     4681 gaaaaccgat atgataaacc gataaaaata attctttacg gtactgataa aatttatgta
     4741 cttgaaaatg taaaacagct gcttcccgta agttttggca aggacagcct gaatagatag
     4801 gattttcgtt ttttttgttg gacaaatata tagtattaca agagtcggaa tacactaatt
     4861 tttgagattg caactttttc gtcgattagt aattctgacc actaaatctt ttaagggatg
     4921 aaaaaggaaa tattttcgat tgagtaccct ctgaataatg cttcagaaat tgttctgtgg
     4981 cgcatgatag gtactccgct gggattggcc gagtggtttt cggatggtgt gactgtagag
     5041 ggtaacgaat atacctttac ctggaaaggg catgagcaaa ttgcttttct caaacagatt
     5101 aaggaaggaa aattaatttc attccaatgg gaagaggata aaaataccga tgcatatttt
     5161 caatttgaaa ttgtcacgct agatttaagc aatcatgtag gccttgtgat taccgatttc
     5221 acaactgcag atgacaaaga agatgctatc cttctgtgga acagtcaggt agaaaccctt
     5281 tgcaggattg gcggcatgta atcgcttcgg gttactgtat ttcgccacta ccaatgcaga
     5341 tatgtgcatc ccggtcgtac accactccaa actggccagc ggcgattccc tgaagtttct
     5401 ctgaagatac tatgcggtaa ccggatgaag ttttgtgtat tttcccttgg gtaaactccg
     5461 gtgtgtgtct tattttgaag cagatatctg cgctttccgc tgtattcatc catggatttt
     5521 cggtaataaa atgaaaatct ttcatttcaa actcgtaccc gtactgtgtt tccacatcgt
     5581 acccttttga tgcccataca atattcgctt ccacatcttt tttgatcaca taccacggtc
     5641 cacccgaaag tccgagtccc ttgcgctgcc ccacggtgtg aaaccagtat ccgtgatggt
     5701 gacccagaat tttgccggtt tccaactcaa caattggtcc ggtttttacc cccaggtagc
     5761 ggcgtataaa ttcattgtaa ttcactttac cgagaaaaca gataccctgg ctgtcctgcc
     5821 ttttggctga tggaagctga gctttgagtg caatatcgcg aacctcgctt ttcatcaggt
     5881 tgccaaccgg gaacatgagt ttggaaacct gcaggtagtt aatctgagcc agaaagtccg
     5941 tctgatcctt tacgggatct ttggcagttc ccaggtatat tttaccgttc tgttctatag
     6001 tggttgcata gtgtccggtg gctgttttgt caaaattgta tcctacacgc tgttcaaaca
     6061 ctccgaattt gatcagttta ttgcacatca catccggatt gggtgtcaat ccctgttttg
     6121 ccttgtcgat ggtataagcc accaccgagt cccagtaatc ctgatgcagg tcaaccactt
     6181 cgagcctgca accgtatttt cgggctatca gacttgccat ctctatatct tcttcggaac
     6241 tgcagtgaag caattcatca tcgtccatcc caattttaat gtagaacaag gtaggagtaa
     6301 ttccgctttc cttcaataga tgtactacta ccgaactgtc aacgccacct gaaattaaag
     6361 tcgctatatt catttttctc tgtcttctta aataaaataa tcagccaaat caatcagacc
     6421 ggcctttacc gcatattttg ttgcttcgtg cacattgttc acccctattt ttcggaatat
     6481 attctttcgg tgactaacca cggtatgcac actcacgtgc cggttttccg aaatctcgcg
     6541 ggtcgttctt cccgaagcca catctttgag tatttctttt tcggttgccg ttagttttgc
     6601 gtcagtaccc tccgattgat gtttataggc actttcaagc agggattttg aaacaatatt
     6661 gctaaaatag cgacgacctt tctgaatttc gtgcaacgcc ataagcagtt cgtgtctgct
     6721 gctgtcttta taaagtatac tgatattcga tgaattaata tatatctgtt ttacaaattc
     6781 atcgctcagt tcgttggaga aaagcagcca gcccgctttc ccgaatccgg cattcaatac
     6841 cagcaattct tctgcacttt gaatatcgaa cagggtataa tcaagcacta caagcgattc
     6901 ggggtgactt tcaagacatg ctatcaattc ctttttgttg tcagcttcgc tgaaacgata
     6961 tgaaccggca gcagagaaga tatgaattaa acctgcccgg ctaatatcct gattatctga
     7021 aagtataatc tgttgcactt tatttgcttt tgtaatacaa ggcacaaagt tagttatttt
     7081 ttcttacaaa aaaagacgac ccattgagcc gtccttttat atctgtacta ttttcaggaa
     7141 ttaatattcc cacaaatcct cttcgaaatt gatcagttcg gtatcaatac gcttttgttc
     7201 ggccctgagc gcgtcttcgt ccacagcata ttccagcagc atacgaccat acgtgctgct
     7261 ttcgccgagc agataactcg aatacatttt ctggttaaag aaatcatcaa atgtcattct
     7321 ttcggtatca tttcccttgg gtaccacaaa ctgacggata agataaggcc gtagttcatc
     7381 aaaggcaaac cagcaaataa ctgagttgcg gaaataagcc atcgattcct tgctgtccat
     7441 gttgtcaggg tgcaatgcat agagcggagc tattgccata attttagaat acatccggga
     7501 agtgtgcttg tcgaaaaata tcttttcctg aatgaggaat ttgagttgat tgcgaatata
     7561 gcgggcgtac tgatccactc ccaccgaccg ttgttcggtg atagtgtcta cttgtaacag
     7621 attgttttca gtagattcat cgtaagcaaa tacgcgtgac agttcgtacc cggtgagcac
     7681 atccttccag cttggtttga tgtcacgcgg gtcacgtttg tacacattaa ttccgttggc
     7741 aacggcatcc agcattaccc tgaaaaggct gcggtactgg tcattagggc gcgtgggaaa
     7801 ataaagctga aaattttgct tatcacgcat atcaatgaca cgatatacag tgcgtgacca
     7861 ggcaatatcg tcccaacgat gattgacaac ggcaattgta tcagccattg catccatttc
     7921 ggcagtttgc agtttttcta ccccaatttc gtcaaaaaac tggagtgttt tctgagcatt
     7981 ggttttgttg gaaacaacaa acaatgaaat gacaaccaat aaaatactat attttttcat
     8041 attgatgaat tgaaaattcg atttgtatgt tattatattt ctatttgaat caagcccagg
     8101 cttcggacct ttccatccgg tcctactgct tttatatttt tcaatgtaac cacatcgcct
     8161 ccttcgagtt cagtcatgag cgacagttga cgcgggctga attctgaact ggaagaagta
     8221 gccgtaccga aaatggtatg aacggtgaat gatgtgatag taaaatttgc tttgaccagt
     8281 tcatcgggac cgtagctggc tatcaggcga ccacttctga gcactttttt cgacatttgt
     8341 gcatcctgcg ttgtccgggg tactcctcct gcatcagtgt aggcaaagta tgctgtcggg
     8401 tccggcagat atttaacgcg atatataccg ctgcccatcg atactgtttt tccctgcatc
     8461 actgccgata cgttgatttt tatttcaccg tcacgggtag gtttgatgat gaagaagttg
     8521 cctgatcgac caacagtagc accatcagcg ctaatacgca ggttttcgtc agggataccg
     8581 ggtgcagaaa tgctgaattt attgtcaatt cccttatata ccacattaag atctacgttg
     8641 gagagtgtgg cagagggttt tcccacaacg tattcgctgg tgaagggata cgattttaca
     8701 tctccctgat tattgggaac tgaaatgctt ccagagtatt tatacgttcc gggtgtagat
     8761 ccgccggcac ggtacacatt gcccgacaat cgtccaccgt tgaccgaata ggtatagctt
     8821 gaagtggtgt caatggctgc aagcacaatt ttagcggtgt aggtacctcc ctgcaaaatg
     8881 taactggact ccggaatgac aaatgcctcc agtttattca ccctgaagtc tttgaggtcg
     8941 gctctggttc gtaaatactg aacagtttcg gcttcggccg aacgcacatc cgcctgatat
     9001 tttgtcagca tggtaatgac agccgcaacc ggcatacttt cgaacatact cgattgccag
     9061 gtttttccga atttattcga ggtatcaaat atctttttgt acatctgttg cttagctata
     9121 tcaccttccg acaattgaat taaaagttcg cgataggttt caattttttt cttcagttct
     9181 ttatagtgtc cgccaaccag tgagtattga cctgctgcat ccaggttatc gcgtgcaata
     9241 atatttacgg cgtttttatc cgctttatcc ttatcggcaa tctttaatat ctgatattta
     9301 taatctccga tatatttgta aagatcgtta gattgagctt ctacatttcg ggcgagttta
     9361 agccattcac caattttttg tggatttttg acagcttctt cctgaaaatc ggcgtacatg
     9421 cctttattcc tgatttcggc cgattcaatt gaatttttca atcccctgtc aaccaatccg
     9481 aatccttcca gaatttcgct cgatacattc agtgcaagca ttgctgtaag caccagatac
     9541 atcatcccaa tcattctctg acgcggggtt tcaggacaat tttttgctcc actcatttgt
     9601 tagtaaattg gttattcagt tattggttat tggttattaa gctctttttc tcttgaaccc
     9661 ccttgagggg gcagggggct ttgtaacttg taacttgctt atgacaatgc attcaacata
     9721 ttgccgtaaa cggcattcaa atcttctacc tgtttggcaa tttttttggt tgtgacctga
     9781 tatttcagac tatcttccag tgtcagtgct gttgcttctt tcattttaag aatatccgag
     9841 gttatatcat ccagatgtcc cgaaacagta cggatatttt ctgattgcag attcagtcct
     9901 tcagtctgcg actgaatgtg gcgcaactga atttcataga ttgaattaat ggacgccaga
     9961 cttttattaa cttctgctac cttatcggca tagctttttg tagtgttggt gatcacatcc
    10021 atttcggatg taatgccctg atatttagca gccagcgttt ctgatgtagt attgagtttt
    10081 tgctgcgtac cggcaaaatt tacagtagct tccgcagctg tttcaatgtt cttagtcaat
    10141 ccgttggcag cgactgctac attggccaga acctgcagat tatcggcagt agcagaaaga
    10201 ttttttattc cttcgctcag tttctttact tcatcgtccg atattgcttc cgtatagttg
    10261 agaccaacct gccttgcggg tgcaacagca gcggtgcttc cgggcgactg tattccagtg
    10321 gccataagat ttagcttttc aggatttttg aaatcaaaaa tctgccccca atcataatct
    10381 ttgtgaggcg tatcaaatat acccaaagcg aaaataaccg attcggtaat cataccggcc
    10441 ataagcatat aattggctcc agctaagtgt aatattttaa acagagcacc gaggattaca
    10501 acagatgcac ctaagctata tgctgctgaa atgattttct tgccacggtt tgaattccac
    10561 attctgtaga atgtgctgtg ttcttgtgcc atcttatact tttatttgat tgatttttta
    10621 tacatggaaa ttttgattta ttcaccgttg taggatcgaa cacagcggaa acctatgtaa
    10681 gggcggcttt cgtactgata ttcgtatgtt ttcactccac actgcaaata ataagctatg
    10741 tctttccagc ttccgccttt taccaccttg cgtttaagta cgtcggggtc gtcatttttt
    10801 gcattgtact gataattggg gttcatatcg gacaccacca tattgcttga cccaccccag
    10861 gcggtagaag tccattccga aacattgcct gccatatcat acagaccgaa attgtttgga
    10921 gcgaatgatc ccactttcat ggttgcagca cccgtatcat cggtgtagct accacgcatg
    10981 ggtttgaaat tggccaggaa acatcctttt gcgtcacgca cataattacc accccaggga
    11041 taaagtgaca aatcacgtcc gccacgggca gcaaattccc actctgcttc agtaggaagg
    11101 cggtaatcct gtcctcccac cgaattggaa ttgttgaaat aattcgtacg ccactggcag
    11161 aaagcctgcg cctgttccca cgaaactccc acaaccggat attgcgaaaa acccttatgt
    11221 gaaaaataca tcttcatttt ggggtcgttg taggcaaact gaaaatcgcg cagccagctg
    11281 attgtatcgg gatacacgtt cacaatgcgg ttggatatca aatcccggcg cgatctgagt
    11341 ttcttggtga tggtaacatt atgaatgaca ccgttttcat ccacgtacga actatccacg
    11401 cgtgccctgg ctcccgggta gtatgcaccg gtattcacgt taaatttatt tccgggtaac
    11461 gctgcctggt cggtgttgag ccattcatac ttatattgca atttagttgg gtcaatctgc
    11521 ctgcgtgaac ccagcagatt attaccatcg taatacatgg aactcaggat ttcgcgcaca
    11581 tcttcatctt tcgaattcca gggaattttt gctttccagt tcaggcgggc ttcttccggc
    11641 gaagcatcat cactctgatt tttgaattgt tttctgtact catcttttcc tgcaatgaca
    11701 agtttgcgga gtgctataga atctttcacc cagttcacaa attgtttgta ctcatcattg
    11761 gtaatttcgg tttcatccat ccagaaggca tccaccgtta cattcatcga tcggtcgtta
    11821 gctccgctga aggctgactg atcattggcg cccatcataa acgatcccct tttcacgaaa
    11881 atcattccgt aaggatttgc ctcgtgaaat gttccggtat tacctacacc aacgagctct
    11941 ccggctgatt tgttgcagga aacaaacagg gaagctacga ataaaattgg cagaaatctt
    12001 ttcattctta ttttgtacat attaataagg aattgattct aatagttatt ttttacaata
    12061 ttctgatact cttgtattta ttcttatttt tactcagatc aaaagcaaaa ctgtatgaca
    12121 aaagcagttc gtgcgatccg gaactcactg tgagcatttt acctgtgggt aactcatagg
    12181 catatcccag ggtaaatccg ctggctaaat tcaatccggc caaaacgatt actgcatcct
    12241 gataccggta tgataaaccg ccccaaaatc ttttatcata ttcaaggcgc gaggacaatt
    12301 ctgcctgcca ggttgtataa tcggttttga gtaaggttga tgtacggagc agtagcttcg
    12361 gattggcaaa tgctgcatcg tatccgccgg tgagatacat cactccacgc acatgaaaga
    12421 tcgatttctc gcccagtttt actgccggag catttaaatg aacgtaagaa atacccgcat
    12481 agtatttcgg agccgaataa aaagcaccaa ccgacatatc caggttcatt ccgttttcat
    12541 cagccgtggg tatggtcata tcgccggaga aatcgtgata ctcggagttg atatccgatt
    12601 tttttacact atcggcaatg aaccctacgc tcacaaatcc cagatctgca cccatactca
    12661 gcactccttt tcctatattc tttttgaaag aatactgcag gccggccgac tgattggtaa
    12721 atgcgccgat tttatcattc aaaaaccgga tacccaatcc ctgagtaacc gttttgctcc
    12781 ctctgaacgg ggcgttgatg gtaaaaaagg ttgtttgagg tgcaccgggc attcccagcc
    12841 actgaatttt atgctgtccg gttacattta tcattcctcc ttcagctatt gctgccggat
    12901 tgtaggttgc cggatgaaac atatactgac ttaactgcgc ttcgaactga gaaaaagcag
    12961 gaagtgtaca agccattgaa aatatcagca atacaatgcg tttcgttttt ctattttcaa
    13021 aaggatttgt catgaactgc ttttttcttt aaatctttat gcctgaaaga ttaaactcca
    13081 aagatttcaa aaaattgtat caacaagcca ttttgttttt catttgtcaa tatcgcgacg
    13141 gcgtaagcag accgggtata tgcaactata tcagctgttt aatactcttc ttcattaaag
    13201 aaaaagtctt ccttgctcgg ataatcgggc cagatatctt cgattgtttc ataaatttcg
    13261 ccttcatctt ctatttcctg aaggttttca ataacttcga ggggcgcgcc cgatctgatg
    13321 gcataatcaa ttaattcatc ctttgtggca ggccagggag catcttcaat ttttgatgcg
    13381 agttctaatg tccagtacat acgctaaatc aaatcttttt aggttaatat cgttaaaatc
    13441 tataaattgg agtgcaaaag tatataatta ttttgttatt ttggttgttt ccacaagatt
    13501 tcttgcattt gatcttccat cagcatgtat cgcgaaagta caaagaaata atcagacaaa
    13561 cgattcacaa aaacaattat tttattttct gtttcataaa cattactcat ttcaataatt
    13621 ctccgttcgg ctctgcgcga aacggtacgg cacacatgac acaaggcact cttgcggctg
    13681 cctccgggaa ggataaagtt tgtcagtggt gtaagttgcg aatccaaccg gtctatttcc
    13741 tgctctatgg ctgtgatgta ttcgtgcttc attaccgatg ccacacggta gcccgtttta
    13801 gtggtatcgg tagccagatt ggaacccacg gtgaacagga ggttctggat gaactgcaaa
    13861 aatgtggtgt gctccgggtt caggtcttca gccacgagca ggccaatctg agcattcagt
    13921 tcatccactg ttccgtaggc ttccagccga atgtcggctt ttgagacccg cgttccgcct
    13981 atgagaccgg tttgcccttt gtcacccgtt ttagtataaa gttttgtcat aaatgtttca
    14041 ttttatagtg cttattgctg agatacgaat cgaaaaagac aagcccgcaa tcaaaaagat
    14101 caatggtaga ttgtacatcc cggtgatttt gaatttcaat ccaggcatct tccatgtctc
    14161 tcgaccagtg aatatcatca acaataaaaa cagaatagcg gctgatatgt tttacgcatt
    14221 tctcaaaata ctgtaatacg gcttctttac ggtggttggc atcaaaaaac accacatcga
    14281 gcgtttcaat cccgtttaat accgcatcaa gcgtttgatc gatatttccg gtcactatct
    14341 ctatgttttt caaccccagc tttctaaaat tatcgcgggc tacagcagcc agttcttcac
    14401 taccttcgag ggtaatacac cgtagctgcg aattggaggc agccagatag gatgtagtaa
    14461 ttccgagtga agtgccgagt tccagaaccg tatttgcctt tgtgaaattt acaattctgt
    14521 acaacaattg cgcctgacgg ggcgatttaa gcgatttgcg cgccacatcg cacacttttg
    14581 aaacagtccg gttgcccgta ccgtaatcgg tgaggtgaag ctgacgctga tcacgcctga
    14641 gtgacgcacg cagtttttca atttcaggaa agatataaaa gggtgttttt tcataaatca
    14701 cgcactgcgt gaatctgaag agatatggag aatgcactcc atgacctttc gtattccttg
    14761 attcgaaaaa atgaattaag tacctgaata taaataaaat taatttcaat ttatcgtttt
    14821 ggttttatat gttcaaatta aagaaacaaa aatacagaaa caaaacaata aatgaaataa
    14881 aaaagcggga ataaagtaat tttattgtgt ctgaggtatt aagttttcat atagtctctt
    14941 ttctttttcg cccggcaggg gttgaaatca cctgaaggta aactacaaaa agccagtacg
    15001 aaagaattcc cgaaagattt tccaggaatt gtctgccgtc atattatttt cagacaattt
    15061 caattccctg ctgttggcac atttccagtg ccatttcgcg aaggcggaat ttctgtattt
    15121 tgccgctgcc ggtcatggga aattgcttga taaagaacac ataacgggga attttgtacc
    15181 gggcgatgtg atttttgcag aaatcgcgca catcttcggg ggtgagttca cagccgtctt
    15241 tgagaataat gaatgcaccc acatcttcgc cgtatttggg tgaagccacc gcaactacct
    15301 gcacatccct gacttcagga attttataca gataatcttc cacttctttg ggcgaaatat
    15361 tttccccgcc ccgaataatc atatccttaa tacgtcctgt aatgcggtaa ttaccgtttt
    15421 cgtcctttac gcccaggtcg ccggaatgca taaatccatt cttatcaatt acctgtgctg
    15481 tggcttcggg atttttgtaa taccctttca ttacgttgta tccccggcag catatctcgc
    15541 cctgcacccc taccggacat tccagaccgg tttcgggatc gagtaccttc acttccgtaa
    15601 attcgtattc acgccctacc gttgtgcatc gcacctcaaa ggtatcgtcc acgcgggttt
    15661 gagtcatgcc gggcgatgtt tcggtcatac catacacgct ggtgatgtcc ataaacattt
    15721 tttcatttac ctggcgcatc agttctatgg gacaaagtgc tccggccata atccccgtac
    15781 ggagcgaact catatcgaaa agactgaaca ttggatgatt aagctctgca ataaacatgg
    15841 tgggcacacc gtgaagcacc gtgcagcgtt ccttgtgaat ggaagcgagg gtaatgagcg
    15901 gatcaaaccg ctccaccatc acctgcgtac tgccatgcgt aaggcagcac atggtggcca
    15961 gcaccacacc aaagcagtga aacagcggta cacagataca gcatttatct tccggggtaa
    16021 atttcatgtg tacaccggtg aggtatccgt tgttggcgat gttgtgatgg gtgagcatca
    16081 cccctttggg aaaaccggtg gttccggagg tgtactgcat attcaccaca tcgtggcaac
    16141 cggtgaggtt gcggagtcgg gtcagttcgt cgctttccac attattgccc agcaggagta
    16201 tttcggccgt attgtacatg ccgcggtatt tttcctgtcc gatgtacacc acgtttttca
    16261 gttccggaaa ccgttcactt ttgaatttac cgcgttgcgt ggttttcagt tcgggcacca
    16321 gctcatagat aatgtcggta tagttatttc cggccactcc gtcagtcata cacagggtgt
    16381 gcatatccga gtttttgagc aggtacgaaa cttcttccga ttggtagttg gtattgacgg
    16441 taactgccac cgcaccaatt ttggccgttg cgtagagaaa tgtaagccag tcgggtacgt
    16501 tttgcgccca gatacccacg ttggttccac ggcttacacc tatggctatc aatccttttg
    16561 ccatattgtc aacacgctcg ttgaaggtct tccaggtgaa gcgcaggtta cggtcggaat
    16621 acacgatata ttctttgtcg ggagtttcag cagcccagtg ttcgagccag tcactcaggg
    16681 ttcggtcgga aagttgcatc tatttaaagt ttgaagtttg aagtttgtaa gccccccagc
    16741 cccctaaagg gggagaaaga aaaaaccagt tacaagttac gagatagaaa ttagaaatta
    16801 gaagttagag gttagaagtc agaaaaaact aattaattca aaattcgcta taattcgtgt
    16861 aacttgcctg ctgcaagcag gttcgtctta attcgtgttc attcgtacat tttagaaagg
    16921 agaataaacg atgcccagaa tttgtgcggg ttgatcgttg gcagtggtaa cgaggtgatc
    16981 tacaatcgaa tcgtagtaaa tgctgtcgcc ctgttcgagg atatactgct ccctgccgta
    17041 ctgcaccagc accgtaccgc tcaacacgta gagaaactct tcaccctcgt gcgaagagga
    17101 tactttttcg ccatcagccg atggtttcag gtcgataaga aacggttcca tgtggcgggc
    17161 tgccttgcgc gatgccagcg agaaaaaatc catatgggag ttggctgttg agagctggct
    17221 cgaaaacgtg gctgcctgtt gtttatcggc actgcggttg actaccggtc cgaagttttc
    17281 actgtcgtcg aggaatgttc cgaggcgcac tccgagtgca cgcgctattt ttatcagtga
    17341 agataatgag ggaatgatct ggctatttag aatttgctga atttgctcca cggttagtcc
    17401 tgtgccttcg gctaattctt ccacagaaat atttttatcg gcacacaacg ttttgatctt
    17461 ttcggttacg gtattcatga gttctggttt atagaaaaat aaattattga tttcggagca
    17521 caaatgtatg tatttttcag tttacagaca aattatattt caatatgttt gtttttgatc
    17581 gttttttatg ttgaattatt tgtatggaat aagttacata atattgcttt tatcttaaaa
    17641 aaatgagttg taagagtgct aacggatttt tcataaaaat aattactttt gtgatactga
    17701 attcaataaa catgtcatgc tatgaaaaca cgtctcttac tcctgttgct tgtggcagtc
    17761 cagct
//
LOCUS       DAOPZK010000035        17638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3373_length_17638_cov_23.874879, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000035 DAOPZK010000000
VERSION     DAOPZK010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17638
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3373_length_17638_cov_23.874879"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="argS"
                     /locus_tag="PKH68_03570"
     CDS             complement(<1..1067)
                     /gene="argS"
                     /locus_tag="PKH68_03570"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNX12050.1"
                     /translation="MNLESILSEGVIQAVSELYGQTISPDIIQFQKTKKEFEGDITLV
                     VFPFVKLARKSPEQTGEEIGAFLKSRFESIEKYNIIKGFVNFTIAPAYWIGQLDEMHH
                     QPEFGIVAAKADADLMMIEYSSPNTNKPLHLGHIRNNLLGFSIAEIQKANGWQVVKTN
                     IVNDRGIHICKSMLAWKLFGNGETPESTGMKGDHLVGKYYVEFDKAYKAEIKMLMDNG
                     KSEEEAKKEAPLIIQAQEMLLKWEQGDEEIRSLWEMMNSWVYAGFDATYRKLGVDFDK
                     IYYESETYTEGKSKVEEGLEKGVFYRRDDGSVWANLIDDGLDEKLLLRSDGTSVYMTQ
                     DIGTAKLRFDDYPIHKMVYVVG"
     gene            complement(1242..1514)
                     /locus_tag="PKH68_03575"
     CDS             complement(1242..1514)
                     /locus_tag="PKH68_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNX12051.1"
                     /translation="MNKAELINAIAETSGMTKVASKKALDATIKSITKALASGEKVGL
                     VGFGTFTVADRAARTGINPSTKKAIKIPAKKVAKFKPGADLADAVK"
     gene            1771..2544
                     /locus_tag="PKH68_03580"
     CDS             1771..2544
                     /locus_tag="PKH68_03580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX12052.1"
                     /translation="MNNYRSSFLSNIPPVVKNILAINVVLFLASVLLPAIFERRGINL
                     DLNDIMGMHYFAAQKFNPAQMITYMFMHASFSHLFFNMFAVFMFGGILERLWGPRKFM
                     FFYLISGLGAAIVQQVFWYFELHTVVSALNTAISSGTFEGLRPYFSGDISAMTISEVL
                     NQKKEFLNMPVTIGASGSVFGLLLAFGWLFPEERLFLMFIPVPIKARWFVLGYAVVEL
                     FLGVAQFSGDSIAHFAHLGGMLFGALLILYWKRKGKLYR"
     gene            2593..3468
                     /locus_tag="PKH68_03585"
     CDS             2593..3468
                     /locus_tag="PKH68_03585"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNX12053.1"
                     /translation="MSIIDNLKLTFKNGNSLIKLIYLNVTVFLLVNILLIVFRLFNAD
                     ASEYIHWLAVPSNVGNLARHFWTPVTYMFLHTGFSHILFNMLMLFWFGRMFLAYYSEK
                     QLLSLYLLGGLGAALVYVVAYNIFPYYSAVAYYSVLMGASGSIMAIVVATAVRAPNAE
                     MNLLLIGRVKLIYIALATVLISVFSLTGKNGGGEMAHLGGALTGYLFVVFDKKGTDIT
                     LFITRVLDFFVNLFRPKPKLKATKFNAQRMSPEEYNQNKARTEVEIDRILDKIKTSGY
                     ESLNAEEKRKLFEKK"
     gene            3485..4582
                     /locus_tag="PKH68_03590"
     CDS             3485..4582
                     /locus_tag="PKH68_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX12054.1"
                     /translation="MTALIKWLLILINIMAVLALGLVKAGSLVSPEKMLLPAYASLFL
                     LPLTLLNIGFVVFWIAGRKWVFLLSMLALIVFYSTIKSAFPINPGKSEVDTTRQKISI
                     LSYNTMNTGLMKKHKPDDPNPVVQYILDTDADIVCLQECAISENKHQFNYDDFERIFK
                     KYPYKHVSFQLNKWSMGIGLVTMSKYPIVEKRNAGYKSIFNLTIYSDIVIGDDTLRIV
                     NNHLESNRITAQDMQMTSKLKDNFDTDELGSVTKYLSQKLSVAYRVRASQADIVAKLI
                     ENSPYKVISCGDYNDVPASYAYTRVLGNLKDAFKETGNGLGWTFAHSFYRFRIDYIMY
                     DKDFQSANYKQGKLRASDHYPIQTDLYLSNN"
     gene            4681..5058
                     /gene="gcvH"
                     /locus_tag="PKH68_03595"
     CDS             4681..5058
                     /gene="gcvH"
                     /locus_tag="PKH68_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNX12055.1"
                     /translation="MNVPSNLKYTKEHEWVRVEGDNAFVGITDFAQGELGEIVFVEVE
                     TVGETLNQGDVFGSVEAVKTVSDLFLPVGGEILEFNEKLEDAPELVNSDPYGEGWMVK
                     IAIADVAELEGLLSAEEYSALLG"
     gene            5221..5727
                     /gene="purE"
                     /locus_tag="PKH68_03600"
     CDS             5221..5727
                     /gene="purE"
                     /locus_tag="PKH68_03600"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNX12056.1"
                     /translation="MTPKVSIIMGSTSDLPVMEKTAKLLDEFQIPFEIHALSAHRTPA
                     EVEKFAKGAQARGIRVIIAAAGMAAHLPGVIASMTTLPVIGVPINASLDGLDALLAIV
                     QMPPGIPVATVGINGALNAGILAVQILAVADDKLQTKLADYKEDLKKKIVKANDELSK
                     VEYKYKVN"
     gene            5733..6059
                     /locus_tag="PKH68_03605"
     CDS             5733..6059
                     /locus_tag="PKH68_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12057.1"
                     /translation="MEVIPMGIYLLYFVYTLPAIFQLIAGTQKLRRKSEHSLLKIAAI
                     SCSSLVVIAGINFLITLFRTKQAQNHDGLWIVGIGALFFMGLILILLISGIQSLILIN
                     QKRKNI"
     gene            6078..7337
                     /locus_tag="PKH68_03610"
     CDS             6078..7337
                     /locus_tag="PKH68_03610"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNX12058.1"
                     /translation="MQTLDNFNFAGKKAFVRVDFNVPVDENFAITDDTRIRAAMPTLK
                     KILADGGRLIIASHMGRPKKNPDPKYSLKVIVDRVSELLEVPVKFAADCMGEETEKMI
                     AELKDGEAVLLENLRFYAEEEGKPRLPETATEEEKAAAKKELKASQKEFTKKLASYAD
                     AYVNDAFGTAHRAHASTALIADYFDADHKMFGYLMNKEVDAVDKLMDNPARPFTAIMG
                     GSKVSTKIEIIENLLTKVDNLIIAGGMTYTFTKALGGKIGISLCEDDKLDLALELIEK
                     AKKNNVNLVLAVDAKIADSFSNDAKTDFVPVGEIPDGWEGLDIGPETEKIFADVIKNS
                     KTILWNGPTGVFEFENFTHGSRSVGEAIAEATRNGAFSLVGGGDSVACVNKFGLVDDM
                     SYVSTGGGALLEAIEGLVLPGIAAIKG"
     gene            complement(7416..8918)
                     /locus_tag="PKH68_03615"
     CDS             complement(7416..8918)
                     /locus_tag="PKH68_03615"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNX12059.1"
                     /translation="MIDQLKLNPNPLVSYLEKLPQDFTKADILRYIEGNGIKMINFRY
                     AGGDGRLKVLNFVINNYAYLDQILTAGERVDGSSLFSFIEADSSDLYVVPRFNTAYLD
                     PFAEIPTLGFLCAYFNKDGNPLESSPEYILQKANRVFTHVTGGMEFYAMGELEYYVIG
                     DEEDAFPAVDQRGYHEAAPFSKFEDFRCKALMYISQVGGQLKYGHNEVGNFVQNGKIY
                     EQNEIEFLPSPVTEAVDQLILGKWIINRLAYEYGLDITFAPKITVGKAGSGMHVHTCI
                     MKEGKNQYVANGQLSDTARKAIAGFMTLAPSLTAFGNTNPSSYFRLVPHQEAPTTVCW
                     GDRNRSALVRVPLGWSSDADMCARANPNEKEEIRDFSIKQTIEFRAPDGSANLYLLFA
                     GLVTAACHGFEMDNALEVAANTYVDVNIHKAGNEKLTNHLEHLPSSCHESAAKLEEMK
                     EIYTRHGVFSERMINGLASQLREFNDSNIRAEVAARPELMQELVDKYFYS"
     gene            complement(9183..9521)
                     /locus_tag="PKH68_03620"
     CDS             complement(9183..9521)
                     /locus_tag="PKH68_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNX12060.1"
                     /translation="MKNNDWKNKLGSLLPAGYIPEPEQEPIKEKTVTTKEPLRVELDK
                     RNGKPATLITEFQGTDDELRQLAKTLKVKCGAGGSQRDGEILIQGDFRVKVAEILMSM
                     GYKVKKINFK"
     gene            complement(9542..10279)
                     /locus_tag="PKH68_03625"
     CDS             complement(9542..10279)
                     /locus_tag="PKH68_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX12061.1"
                     /translation="MKFYKYFLLLLACSVTLFSHGQGNLQKDLYQAFLLNDAAKWEKV
                     TYNYEKNADLSSNVDLLKLIHCYYGYISVLIDKDQDKKATENLKKAETYIDKILKEEP
                     NNAAALNYKGVFISYYVSMNKMKAPALGNQILTNLNKAYKLDPNNVQILFDRGNSLFY
                     PPKMFGGNKTEALKYYQKAISIIEKQNNTRENWIYVQLLFQVGRCHELTGNLELAKKE
                     YEKTLKIEPNLKLLRDKYYPGLLNKMG"
     gene            complement(10276..10821)
                     /locus_tag="PKH68_03630"
     CDS             complement(10276..10821)
                     /locus_tag="PKH68_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX12062.1"
                     /translation="MRKLKITELNRLTTEEFKLRKKTPLVIVLDNVRSLHNVGSVFRT
                     SDAYLVETVCLCGITSTPPHAEIHKSALGAENSVDWVYFEDTLEAIKELKTKGFEVLA
                     IEQAEGSTLLNQLEINPAGKYAVVLGNEVKGVQQAVVDACDGCIEIPQYGTKHSLNVS
                     VTAGIIIWEFASKLFNNKPLI"
     gene            complement(10860..11870)
                     /locus_tag="PKH68_03635"
     CDS             complement(10860..11870)
                     /locus_tag="PKH68_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX12063.1"
                     /translation="MKKIYSILIFSSILAFQSCMEEPEIRPNTPQGNFKSLWEIIDTR
                     YCYLSYKNIDWDSVYTAYKPRVDTVSGASSLFDLMSEMLAMLKDGHVNLYSEFDRSRY
                     WNWYSDYPSNFNSEVLFKPHYLGSHYRIASTLRYNKIARGKVGYVYYGSFSDGFSDQN
                     MNAIFSSFASCEGIIIDIRNNGGGMLSYAEQMASYFFKEKTLTGYISHKTGSGHTDFS
                     KPTPLYVTPNENVKWNKPVIVLTNRMSYSATNDFANKMRYAPQATIIGDQTGGGGGMP
                     FSSELPNGWMIRFSSSPMYDADMQNIEWGIAPDIRVSMDLVALSKGYDTIIERAIAEI
                     VK"
     gene            complement(11901..12749)
                     /locus_tag="PKH68_03640"
     CDS             complement(11901..12749)
                     /locus_tag="PKH68_03640"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HNX12064.1"
                     /translation="MRKALFLFLWIFTVQTIGAQDLTEKKFPLTTSVKTVNVGNIRVL
                     DPYLSPFEYSGFLVGYKEDNRRFLFSSDTTLSFTSRKMLNVGSADHPNKNNSMLMFNG
                     NYNLGLNYHIRPADKLMILIGGSWDIDLGGKYIGRNVNNPFSLDLYTNINAAAEIQYS
                     FNIRRQHFRLQYGAQTPLIGCMFVPMQGASYYELFSLSNLTDAIHLSSLVNNRAVMQY
                     FHLDLPLKYTTFRFGVQHDYMQYAANDMVFHKKGFSFSIGYLVDLYSFPGKRNEMPGN
                     FISAYK"
     gene            12896..13366
                     /gene="queF"
                     /locus_tag="PKH68_03645"
     CDS             12896..13366
                     /gene="queF"
                     /locus_tag="PKH68_03645"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNX12065.1"
                     /translation="MEKEKLTLLGGKTEYKNDYAPEVLGTFENKHPGNDYWVTFDCPE
                     FTSLCPITGQPDFATIRIDYIPAVKMVESKSLKLYLFSFRNHGAFHEDCVNIIMKDLV
                     RLMDPKYLEVTGIFTPRGGISIYPYANYGRKDTEFEKLARERLFQHSFPINRQL"
     gene            13363..14064
                     /locus_tag="PKH68_03650"
     CDS             13363..14064
                     /locus_tag="PKH68_03650"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNX12066.1"
                     /translation="MNNRKKLVVLTGAGMSVESGLSTFRGSGGMWGQHRVEDVATIDA
                     WYRNKQLVLDFYNMRRKELLGVKPNQGHIALAELEKDFDVEIITQNVDNLHEQAGSKR
                     VLHLHGELMKVRSTGPGAEVYDLIPENYITREGDLCPGGYQLRPHIVWFGEAVPEMEH
                     AIDIVQNADIFLIIGTSLQVYPAAGLLNYVASQVPVFMIDPNEVGTYSRKITVIKKGA
                     GDGLKELKELLKNFL"
     gene            14208..15287
                     /locus_tag="PKH68_03655"
     CDS             14208..15287
                     /locus_tag="PKH68_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12067.1"
                     /translation="MIKDKKKILFILLYLFLSFSVGSQPAVPTHGQEHELTLYIMPTL
                     EPLDWTSPKSIYTSTFHCYLKNITTKDNYLLGHVAIKLKSSLLDKPLLTTQTSSGMQE
                     KLDLIFKQKIGLGILGAPITGRMEPSTELSRKLKVYSERNKLTFIRYNINEKAARRII
                     EFVEQYTQVRDSSFATCNYYGGSFWPLNKNEGAGCSSFGIAVLQLAGIEPIEPEKWIV
                     EVKIPMKLIGGSLNNNKKIKINEIKKTNQWYEGRGVANVDYVNYKVYDPSLMFDWVLN
                     MTRQPSSHYRVISDEGTKGLLIDATAAVVDENEPMFSPNKEADLFVTHYLQKIPRQLQ
                     TNEGQDTTQSSGIPEHLFLQYPPRN"
     gene            15444..15635
                     /gene="rpsU"
                     /locus_tag="PKH68_03660"
     CDS             15444..15635
                     /gene="rpsU"
                     /locus_tag="PKH68_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNX12068.1"
                     /translation="MIVVPVKEGENIEKALKKFKRKFEKTGVVKELRRRQCFDKPSVV
                     KREKKMHAVYVQHMDRNED"
     gene            15719..16618
                     /locus_tag="PKH68_03665"
     CDS             15719..16618
                     /locus_tag="PKH68_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX12069.1"
                     /translation="MINKFLQYLQYEKNYSSHTVLSYQTDLLQFCDFLSIDSLDFSPA
                     GVTPLEIRQWVMHLINNNYTAKSVSRKISALKSFWRFLLIHGLAEKNPTLKIVLPKTK
                     KLLPAFYKQQEMERALAEDTLPGGFESLRNRLIISLLYEAGLRVSELTSLTDEDFDFA
                     SQQIKVIGKRNKQRIIPLGGDICEMISEYKQLRNKEVERQNNALFLLSSGLPMYSKAV
                     YNIVHSEMSQVSTLHKQSPHVLRHTFATTLLNNGADINAVKDLLGHSSLAATQVYTHV
                     TFSELNKIYKQAHPRAQKKGGNL"
     gene            16615..16914
                     /gene="raiA"
                     /locus_tag="PKH68_03670"
     CDS             16615..16914
                     /gene="raiA"
                     /locus_tag="PKH68_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNX12070.1"
                     /translation="MKVMIQSINFDATEKLEEYVNKKAKKIEKFFDEIRIIDVYLKVI
                     KPETATNKQAEIKVTAPNVEFFASKTCDTFEEAVDLALEAIEKQVIKFKEKIAKK"
     gene            17026..17099
                     /locus_tag="PKH68_03675"
     tRNA            17026..17099
                     /locus_tag="PKH68_03675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17060..17062,aa:Thr,seq:tgt)
     gene            17242..17324
                     /locus_tag="PKH68_03680"
     tRNA            17242..17324
                     /locus_tag="PKH68_03680"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17276..17278,aa:Tyr,seq:gta)
     gene            17398..17470
                     /locus_tag="PKH68_03685"
     tRNA            17398..17470
                     /locus_tag="PKH68_03685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17431..17433,aa:Gly,seq:tcc)
BASE COUNT         5002 a         3638 c         3827 g         5171 t
ORIGIN      
        1 ccaaccacat acaccatttt gtggatgggg tagtcgtcga agcggagttt ggcggtacca
       61 atgtcctgtg tcatgtacac cgatgttccg tcggagcgca gcaatagttt ctcatccaga
      121 ccgtcgtcaa tcagattagc ccacacagag ccatcgtcac ggcggtagaa aacccctttt
      181 tccaatcctt cttccacttt tgattttcct tcggtgtaag tttcggattc gtaatatatt
      241 ttatcaaaat ccactccgag tttccggtat gttgcatcaa atcctgcgta cacccagctg
      301 ttcatcattt cccacagtga gcgtatctct tcatcgccct gttcccattt cagcaacatt
      361 tcctgtgcct gtatgatgag cggagcttct tttttggctt cttcttcact cttcccattg
      421 tccatcagca ttttgatttc tgctttgtaa gccttgtcga actccacgta gtatttccct
      481 acgaggtggt cgcctttcat gccggtgctt tcgggtgttt ctccgttgcc gaatagtttc
      541 caggcaagca tggatttgca gatgtgaatg ccgcggtcgt ttacgatatt ggttttcacc
      601 acctgccagc cgttggcttt ttgaatttcg gcaatactga atccgagcag attgttgcgg
      661 atatggccga ggtggagcgg tttattggtg ttgggcgaag aatattcaat catcatcagg
      721 tcggcatctg ctttggctgc tacgattccg aattcaggct gatgatgcat ttcgtcaagc
      781 tggccgatcc agtatgccgg agcgatggtg aagttgacaa atcccttgat aatgttgtat
      841 ttctcgatac tttcgaaccg ggatttcaga aaagcaccga tttcttcacc cgtttgttcg
      901 ggcgatttgc gggccagttt tacaaatgga aaaaccacca gggtaatatc gccttcaaat
      961 tccttctttg ttttctgaaa ctgaatgata tcaggtgata tcgtttgtcc atacagttcg
     1021 cttacggcct gtattactcc ttctgaaagt attgattcta agttcatttt cggtgtgtta
     1081 atgtttctgt atttatttga caggctgaaa tgcggaaaag tgcattcagt ctgtttgctg
     1141 gttgaaagtg caaagataca attttctgct gtttcgggaa aataaaaaag ggcactaacg
     1201 ggtagtgtcc ttcttttaat gcgtttgggg agtggctgtt attatttaac agcatcggct
     1261 aaatcggcac cgggtttgaa ctttgcaact tttttggcag ggattttaat cgcctttttg
     1321 gttgatggat tgatacctgt gcgggctgca cgatcagcaa cagtaaatgt tccgaaacct
     1381 accagaccaa ctttttcacc gctggcaaga gctttggtga tacttttgat ggtagcatca
     1441 agcgcttttt tagaagcaac cttagtcata ccggatgttt ctgcaattgc attgattaat
     1501 tcagctttgt tcataattta ctaagttttt agattataag ttattaagca tagacattat
     1561 gccacaaatg taaaaaattg aaaaacaaaa aacaatcttt tcgacaaata aatgttgatt
     1621 ttttgtaaaa attcttcatt ttatgttcaa aaacattgaa ttggagtttt tttagtaatt
     1681 ttgcacactt aaaaaccagg acacgacggg caaatcggat ttcatttcag taatttgcct
     1741 ctgattaccg gtttgaaaaa atactgattt atgaacaact accgatcttc ttttttgtca
     1801 aatatacctc ctgttgttaa aaatatactt gccatcaatg ttgtgttgtt tctggcatct
     1861 gtattacttc ccgctatctt tgagcgacgc ggcatcaacc tcgatttgaa cgacatcatg
     1921 ggcatgcatt attttgcagc tcagaagttc aatccggcgc agatgatcac ctatatgttt
     1981 atgcacgcct cgttttctca cctttttttc aatatgtttg ccgtctttat gttcggtggt
     2041 attctggaaa ggttgtgggg accgcgaaag ttcatgtttt tttacctcat cagcggactg
     2101 ggtgctgcca tcgttcagca ggtattctgg tatttcgaac ttcatacggt ggtatctgcc
     2161 ttaaatacag ctatttcatc cggcactttc gagggattac gaccctattt cagcggtgat
     2221 atctcagcaa tgaccatttc ggaggtgtta aatcaaaaaa aggaatttct caatatgccg
     2281 gtcaccatcg gtgcatcggg gtcggtgttc ggattgctgc tggctttcgg ctggcttttc
     2341 cccgaagagc gcctttttct catgtttatt ccagtgccca tcaaggcgcg atggtttgtg
     2401 ctcggttatg ctgtggtgga actgtttctg ggagttgctc agttttcggg cgacagtatt
     2461 gcgcattttg cccacctggg cggtatgttg ttcggcgcgc tgcttattct ttactggaaa
     2521 agaaaaggaa aactttaccg ctgaaaaatc tgtaactgtt ctcaggtcgt tatctttaat
     2581 gtctaaaaat caatgagtat catcgataat ctcaaactga cctttaaaaa cggaaattcg
     2641 ctcatcaaat tgatttatct caatgtgacg gtatttctgc tggtcaatat actgctgatt
     2701 gtattccggc tgttcaatgc agatgcttcg gagtacatcc actggctggc tgtaccctcc
     2761 aatgtcggaa atttggcccg tcatttctgg acaccggtta cctatatgtt tcttcacacc
     2821 ggtttctctc acattttatt taatatgctc atgctttttt ggtttggcag gatgtttctg
     2881 gcctattatt ccgaaaagca actgctgtca ctttaccttc tcggggggct gggtgcagct
     2941 ttagtgtacg tggtggctta taatattttc ccctactatt cggcagtggc atattacagc
     3001 gtgttgatgg gagcatcggg gtcaattatg gctattgtgg tggctactgc cgtgagagcg
     3061 cccaatgccg aaatgaattt gttgctcatc ggtcgtgtga aactgattta tatagcactt
     3121 gccacggttc tcatcagtgt tttcagccta acgggcaaaa acggaggcgg ggagatggct
     3181 cacctgggag gggcattaac aggctattta tttgtggttt ttgataaaaa agggacagat
     3241 attacacttt ttattacccg cgtattggat ttctttgtaa atttgttccg tccaaagccg
     3301 aagctcaaag cgaccaaatt caatgcccag cggatgtcgc cggaggaata caaccaaaac
     3361 aaagcccgca cagaagttga aattgatcgc atacttgaca aaattaaaac ttcgggttac
     3421 gaaagtctga atgccgaaga aaaacgtaaa ctttttgaaa aaaaataaga ctaaaagcag
     3481 accaatgacc gctttgatta agtggttgct tattctgatc aatatcatgg cagttttggc
     3541 tttgggattg gttaaagccg gctcgctggt cagtcccgaa aaaatgctgc taccggctta
     3601 tgcatcgctt tttctccttc ccctgacgct tctcaatatc ggttttgtgg tgttttggat
     3661 tgccggtcgc aagtgggtgt ttttgctctc gatgctggcc ttaattgttt tttacagcac
     3721 cataaaatcc gcctttccca tcaatcccgg taagtccgaa gtggatacta cccggcagaa
     3781 aatcagcata ctttcataca ataccatgaa tacgggattg atgaaaaaac ataaacccga
     3841 cgatcctaac ccggtggtcc agtacattct ggataccgat gccgatatcg tgtgcctgca
     3901 ggagtgcgcc atcagcgaaa ataagcatca gtttaactac gacgatttcg aacggatatt
     3961 taaaaagtat ccctacaagc atgtgagttt tcagctcaac aaatggagca tgggtatcgg
     4021 acttgtaact atgtcgaaat atcctatcgt cgaaaagcga aatgcaggct acaaatccat
     4081 atttaatcta accatttact ccgatattgt cattggtgat gatacgctgc gcattgttaa
     4141 taatcacctc gagtccaacc gaataaccgc tcaggatatg cagatgacct caaaactgaa
     4201 agataacttc gataccgatg aacttggatc ggtaaccaaa tacctgtcgc aaaaactgag
     4261 tgtggcttac cgtgtacgag cctcacaggc cgatatagtg gcaaagctga tagaaaattc
     4321 tccctacaag gtgatttcgt gcggcgacta caacgatgtg cctgcatcat acgcttatac
     4381 cagggtgctt ggaaatctca aagatgcctt caaagagacc ggcaacgggc ttggatggac
     4441 atttgctcat tccttttacc gttttcgtat cgattacatc atgtacgata aggattttca
     4501 gtctgccaat tataaacagg gcaaactgag agcttccgat cattatccca tacaaactga
     4561 tttgtattta agcaataatt aactacaatg ctgtttgttt tttttgtaac tttgttgctc
     4621 ttttattgca gtgaaatatc ataaactaaa aaatatatac aactttaaaa aactaatctt
     4681 atgaatgtac catctaacct gaagtacacc aaagaacatg aatgggtacg cgttgaaggc
     4741 gataacgcct ttgtgggaat caccgacttt gcacagggcg aattaggcga aattgttttt
     4801 gtggaagtgg aaactgtggg cgaaactctc aaccagggtg acgtattcgg ctctgtggaa
     4861 gctgtcaaaa cagtttctga cttgtttctg cctgttggcg gagaaattct ggaattcaac
     4921 gaaaaactgg aagatgctcc cgaactggtc aacagtgacc cgtatggcga aggctggatg
     4981 gtgaaaattg ccattgccga tgttgccgaa ctggaaggat tgctttctgc agaggaatat
     5041 agtgcattgc tggggtaaga gactggcccc cttaccccct aaagggggaa ttttgaatgc
     5101 ttactgataa aattattaag tatttatcaa aataaaataa agggatctta atttaaaata
     5161 aatttgaaat ttgcttttta ttcccccttt agggggttag ggggcagact acaggaaatt
     5221 atgacaccaa aagtaagtat tatcatgggc agtacgtccg accttccggt gatggaaaag
     5281 acggctaaac tgcttgatga atttcaaatt ccgtttgaaa ttcatgcttt gtcggcacat
     5341 cgcacgccgg ccgaagtgga gaaatttgca aagggagctc aggcaagagg tattcgggta
     5401 ataattgctg ctgccggaat ggctgctcat cttcccgggg tgattgcttc catgaccacg
     5461 ctgccggtga ttggtgttcc catcaatgct tcgctcgatg gtctggacgc tttgctggcc
     5521 atcgtacaga tgcctccggg catacccgtt gctaccgtgg gtatcaacgg agctctgaat
     5581 gccggtatcc ttgcagttca gatacttgct gtagccgacg ataaactaca gactaaactg
     5641 gctgattaca aggaggatct gaaaaagaaa attgtaaagg ctaacgatga actgagtaag
     5701 gtggaatata aatataaggt gaattaagcg atatggaagt cattccaatg ggtatttatt
     5761 tattatattt tgtttatact ttaccggcaa tatttcaact gatagccgga acacaaaaat
     5821 taagaagaaa atcagaacac tcgctgctga aaattgcagc aatcagctgt agtagtcttg
     5881 tcgtaattgc aggtataaat tttcttatta cattattcag aacaaaacaa gcccaaaatc
     5941 acgatggatt atggattgtc ggaataggtg cattgttttt tatgggacta attttgattt
     6001 tacttatttc aggcattcag agcctgattt taataaacca aaaaagaaag aatatataac
     6061 ttaaaaataa attgattatg caaacattag acaactttaa ttttgccggt aaaaaagcct
     6121 ttgttcgcgt ggactttaat gttccggttg acgaaaactt tgcgattacc gatgatacac
     6181 gtatccgggc tgccatgccg actttgaaaa aaattctggc tgatggtggt cgtctgatca
     6241 tcgcttcaca catgggccgt ccgaaaaaaa atccggatcc gaaatactcc ctcaaggtaa
     6301 ttgttgaccg tgtatctgaa ttgctggaag ttcctgtaaa atttgctgct gactgtatgg
     6361 gtgaagaaac tgaaaaaatg attgctgaac tgaaagacgg tgaagccgta ttgctcgaaa
     6421 atctccgctt ctatgccgaa gaagaaggta aaccccgttt gcccgaaacg gccaccgaag
     6481 aagaaaaagc tgctgcaaaa aaagaactga aagcttcgca aaaagaattt accaaaaagc
     6541 tggcatcgta tgccgatgct tatgtaaatg atgcttttgg aacagctcac cgtgcccatg
     6601 cctctaccgc attgattgct gattattttg atgctgacca caaaatgttc ggttacctga
     6661 tgaataagga agtggatgca gtagataaac tgatggacaa tcctgcccga ccattcacgg
     6721 ccatcatggg tggttccaag gtttccacca aaatcgaaat cattgaaaat ctgctcacca
     6781 aagtggataa tctgattatt gccggtggta tgacttatac ttttaccaaa gcacttggag
     6841 gtaaaatcgg tatttcgctg tgcgaagatg acaaactgga cctggcattg gaattaattg
     6901 aaaaagcaaa aaagaacaat gtaaaccttg tattagctgt cgatgcaaaa attgcagact
     6961 ccttcagcaa tgatgcaaaa actgatttcg ttcccgtagg tgaaatcccc gacggttggg
     7021 aaggtcttga tatcggtccc gaaactgaaa agatttttgc tgatgtaatt aaaaattcca
     7081 aaactattct ctggaatggt cctaccggtg tgttcgaatt cgaaaacttc acacacggct
     7141 cacgctcggt aggtgaagct atagctgaag ccacccgcaa cggtgcattc tcgctcgtag
     7201 gcggcggcga ctctgtagct tgtgtcaata agtttggtct tgtcgacgat atgtcgtatg
     7261 tttccaccgg tggcggagcg ttgctcgaag ccattgaagg attggtgctg ccgggaattg
     7321 cagctatcaa aggataaaac accgaaaata caaactaaaa aagccggacg atgaaaatta
     7381 tccggctttt ttctgtgttt ttcaatgtga gaaggttagc tgtaaaagta tttgtccacc
     7441 aattcctgca tcaactccgg ccgggcagcc acttcggcgc ggatattcga gtcgttgaac
     7501 tcccgaagtt gacttgccaa tccattaatc attcgctcgc tgaacacacc atgacgggtg
     7561 tagatttctt tcatttcctc cagtttagca gcagactcat gacatgatga cggtaaatgt
     7621 tcaagatggt tggtcagttt ttcgttaccg gctttgtgaa tgttgacatc cacgtaggtg
     7681 ttggcagcaa cctccagtgc attgtccatt tcaaaaccgt ggcaggctgc tgttaccagt
     7741 ccggcaaaca gcagatacag attggccgaa ccgtcgggtg cccggaattc aatggtttgc
     7801 tttatcgaaa aatctctgat ttcttctttt tcattcggat tagccctggc gcacatatcg
     7861 gcatcggagc tccagccaag tggaacgcga acgagtgccg atcggttgcg gtcgccccag
     7921 caaacggtgg taggagcttc ctgatgtggc accagccgga aataagagct cggattggtg
     7981 ttgccaaaag ctgtgagcga gggtgccagt gtcataaatc cggcaatggc ttttcgggcc
     8041 gtatcactca actgaccatt tgcaacatat tgatttttac cttctttcat gatgcaggtg
     8101 tgtacatgca tgccgcttcc ggcttttccc accgtaattt tcggagcaaa agttatatcc
     8161 agtccatatt cgtaggccag tctgttgatg atccatttac ccagaattaa ctggtcaaca
     8221 gcttcggtta cgggcgatgg cagaaattca atttcattct gttcgtagat ttttccgttt
     8281 tgcacaaagt tacccacttc gttgtgtccg tatttgagct gcccgccaac ctgcgaaatg
     8341 tacatcaggg ctttgcacct gaaatcttca aatttgctga agggtgctgc ttcgtgatat
     8401 ccacgctgat caacagccgg gaatgcatct tcctcatcac caatcacata atattccagt
     8461 tcgcccatgg cataaaattc cattccgcca gtcacatggg taaacacgcg gttcgccttc
     8521 tgaagaatat attccggtga actttcgagc ggatttccgt ctttgttgaa gtatgcacac
     8581 agaaaaccga gagttggaat ttcggcaaac ggatcgaggt aagccgtatt gaagcggggt
     8641 actacatata aatccgacga atcggcttcg ataaacgaaa agagactgga gccgtccacc
     8701 cgttcgccgg cagtcaatat ctggtcaagg taagcgtaat tattaatcac aaagttgaga
     8761 actttcagtc ttccgtcgcc acctgcgtac ctgaagttga tcattttaat gccattacct
     8821 tcgatgtagc ggaggatgtc tgcttttgta aagtcttgag gaagtttttc cagataagaa
     8881 accagtggat ttggattgag tttgagttga tcgatcatga tataatgata ttatattaaa
     8941 ggtttgtggt gcaaatataa ttcatttatt tcttaaaatg gcatgacgag caattaaaag
     9001 ctgacaaaat gataaaaata ccgggatttt tttcgaagaa aagcacattg tgatttattc
     9061 ggacaatgtg gcacttctct ttttcggaat aattttaaaa aaaatagagt gggtagagtt
     9121 tgggtaacag gttggatgag tgtgttaaat ttaatttaca tatagttctg tataccaata
     9181 ggttatttga aatttatctt ctttacttta tagcccatac tcatcagtat ttcagccact
     9241 ttcaccctga aatcgccctg aatgagtatt tcaccatcgc gttgtgagcc gccggcgcca
     9301 catttcactt tcagtgtttt ggcaagttgc ctgagttcat cgtcagttcc ctgaaactcc
     9361 gttatcagag tagccggttt accgttacgt ttgtcgagtt ccacgcgtaa aggttccttt
     9421 gtagttacgg ttttctcctt tattggctct tgctcaggtt ccgggatgta accggcaggc
     9481 aacagggaac ctaatttatt tttccagtcg ttatttttca tatttttttt ccaaagcaaa
     9541 attaacccat tttatttaac aaaccgggat agtatttatc tcgcagcaat tttaaattgg
     9601 gctctatttt caacgttttt tcgtactctt tttttgccaa ctcgaggttt cccgtcagtt
     9661 catggcatcg tcctacctga aaaagcagtt gcacatatat ccagttttcg cgggtgttat
     9721 tctgcttttc gataattgat attgctttct ggtaatattt cagggcctca gttttatttc
     9781 ctccaaacat tttgggtggg taaaacagcg aattgccccg gtcaaaaaga atttgcacat
     9841 tatttgggtc caatttgtag gctttattta aattagtcag aatttgattt ccgagtgctg
     9901 gagccttcat tttgttcatt gaaacataat agcttataaa tacacctttg tagttcagag
     9961 cagcagcatt gtttggctct tcctttaata ttttatcgat gtaggtttca gcttttttta
    10021 aattctcagt tgcctttttg tcttgatctt tatcaatcaa aacggaaata tagccataat
    10081 aacaatgtat aagtttcagc aaatcaacat tgctactcaa atctgcattt ttttcataat
    10141 tataagttac cttttcccat tttgcagcat cattgagcaa aaaagcctga tagagatctt
    10201 tttgaagatt gccctggccg tggctgaaaa gtgttactga gcatgctaaa agtaagagga
    10261 aatatttata aaatttcata ttaatggctt attgttaaat aatttagaag caaattccca
    10321 gatgataatt cctgctgtaa cgctcacatt gagtgaatgt ttagtgccgt actgaggaat
    10381 ttcgatacag ccgtcgcagg catccactac ggcctgctgc acgcctttca cctcgttgcc
    10441 cagcacgact gcatatttac ctgccgggtt gatttccagt tggttgagca gggtgctgcc
    10501 ctcggcctgc tctatagcca gtacttcaaa tccctttgtt ttcagttctt tgatggcttc
    10561 cagcgtatcc tcaaaataga cccagtccac cgaattctca gctcccaatg ccgacttgtg
    10621 tatttcggcg tggggtggag tagaagtgat gccgcacaga cagactgttt ctaccaggta
    10681 ggcatcggaa gttctgaaca ccgaaccgac attgtgaaga ctgcgcacat tgtcgagcac
    10741 aataaccagt ggtgtttttt ttcgtaattt aaattcctcg gtagtaagcc ggttgagttc
    10801 ggttattttc agttttcgca taattttcag gtaattgcat tattccaaca tagaaaaatc
    10861 tacttaacaa tttcggcgat agcccgttca ataatggtgt cataaccctt ggacaatgcc
    10921 accagatcca tagatacccg aatatcgggc gcaattcccc attcaatatt ttgcatatcg
    10981 gcatcataca tcggactgct cgaaaaacgg atcatccagc cgttgggcag ttcactggag
    11041 aagggcattc caccgccacc accggtctgg tcaccgatga tggttgcctg tggagcatat
    11101 ctcattttat ttgcaaaatc attcgtagcg gaatacgaca tcctgttggt aagaacaata
    11161 accggcttat tccacttgac attttcgttt ggagtaacgt aaagaggtgt tggctttgaa
    11221 aagtcggtat gtcctgatcc tgtcttgtgg cttatatatc cggtgagtgt tttttcctta
    11281 aaaaaatatg aagccatttg ctcagcgtag ctcagcatgc cgccgccgtt attgcgaata
    11341 tctataatta ttccctcgca ggaggcaaaa ctggagaata ttgcattcat gttttggtct
    11401 gaaaatccgt cagaaaagct tccgtaatat acatatccta cctttccgcg ggctatcttg
    11461 ttgtatctta atgtacttgc tatccgatag tgtgatccca ggtaatgtgg tttgaacaac
    11521 acttcggaat tgaaatttga cggataatcg gaataccagt tccagtaacg actgcggtcg
    11581 aattctgaat ataggtttac gtgaccgtct ttcagcatgg cgagcatttc gctcatcaga
    11641 tcgaagagtg aagaggcacc cgaaaccgtg tcgacccgcg gtttataggc agtgtaaacc
    11701 gaatcccagt cgatattctt gtaactcagg taacaatagc gtgtgtcaat tatttcccaa
    11761 agtgatttga aattgccctg aggagtattt ggccgaattt caggctcctc catgcaggac
    11821 tgaaatgcca aaatcgaact gaagattagt atgctgtata tctttttcat tccgtatcaa
    11881 tgacaacact cttataccat ttatttgtag gcggagataa aattaccggg catttcattt
    11941 cttttacccg ggaatgagta cagatctacc agatagccga tcgaaaaact gaagcctttt
    12001 ttgtgaaaaa ccatatcatt agctgcatat tgcatgtaat catgttgcac accaaagcgg
    12061 aaagtggtat atttcaacgg caaatccagg tgaaaatact gcatcactgc gcggttattg
    12121 accagactgc tcaggtgaat agcgtcggtc aggttgctca gactgaacag ctcataatac
    12181 gaagcaccct gcataggaac aaacatacac ccaataagcg gtgtctgagc gccgtattga
    12241 agcctgaaat gttgacggcg aatgttgaaa ctatactgaa tttcggcagc agcattaata
    12301 ttggtataaa ggtcaagcga aaagggattg tttacattcc gaccgatata ttttccgccc
    12361 agatcgatgt cccacgatcc gccaatcaga atcatcagtt tatcggcagg acggatgtgg
    12421 tagttgagcc cgaggttgta gttgccgttg aacatcaaca tggaattatt cttattcggg
    12481 tgatcggcgg agcctacgtt gagcattttt cggctggtaa aagaaagtgt ggtgtcagaa
    12541 gaaaatagaa agcgtctgtt atcttcctta tagccaacca gaaaaccact gtattcgaaa
    12601 ggcgacaaat agggatcgag tacacggatg tttcccacat tcacggtttt gaccgatgta
    12661 gtgagcggaa atttcttttc cgtcaaatcc tgtgccccga ttgtctgaac cgtgaaaatc
    12721 caaagaaaca gaaaaagtgc ttttctcatc tgataagcta aaagtgaatg attatattgc
    12781 aaaaatataa aaaataagcc aagtcgttgg ggttttgttc caaaaaaaga ggaaacattt
    12841 gtattttccc cggattgaaa gtaaatttgt gaaatcatta aaatggatac agagtatgga
    12901 aaaagaaaaa ctgacgctgc ttggcgggaa aaccgaatat aaaaatgact atgctccgga
    12961 agtactcgga acgtttgaaa acaagcatcc cggcaacgat tattgggtca cttttgattg
    13021 tccggagttc accagtctct gtcccataac cggtcagccc gattttgcta ctatccggat
    13081 tgactatatt cccgctgtga agatggttga aagtaaaagc ttgaagctct atttgttcag
    13141 tttccgtaat catggcgcat ttcacgaaga ttgtgtcaat attataatga aggatctcgt
    13201 ccggctgatg gaccccaaat acctggaggt gaccgggatt tttacgcctc ggggcggcat
    13261 aagcatttat ccctatgcca attacggtcg taaagacact gaatttgaaa aattagccag
    13321 ggagagattg ttccagcatt cttttccaat aaacagacaa ctatgaataa tcgaaaaaaa
    13381 ctggtggtac tgaccggtgc aggtatgagt gttgaaagtg gattgagtac attccgcggc
    13441 tcgggtggta tgtgggggca acaccgggtg gaagacgtgg ctaccattga tgcctggtat
    13501 aggaataaac aacttgtatt ggacttctac aatatgcggc ggaaagaact tctgggtgtt
    13561 aagcccaatc agggtcatat tgctctggcc gaactcgaaa aggattttga tgtggaaatt
    13621 attacacaaa acgtggacaa tctgcatgaa caggctggaa gtaagcgggt attgcacctg
    13681 cacggtgagt tgatgaaagt gcgttccacc ggtcccggag ccgaggttta tgatttaatt
    13741 cccgaaaatt atattacccg cgagggtgat ctgtgccccg gggggtatca gctccggccg
    13801 cacattgtgt ggttcggtga agccgtaccc gaaatggaac atgcaataga tattgtacaa
    13861 aatgcagata tttttctcat tatcggcacc tcgctgcaag tatacccggc tgcaggcttg
    13921 ttgaattatg tagcatctca agtgcctgtt ttcatgatag atcccaatga ggtgggaaca
    13981 tacagccgga aaatcactgt gattaaaaaa ggggcaggcg atggattaaa agaactgaaa
    14041 gaacttctga aaaattttct gtaacttagt catagaggtg aaaatccgaa attaataccg
    14101 gaataaattg gtaattcgaa ctttaatttc tatctttaca ccgcttaact gccttgtggt
    14161 attatttacc accattattc atctgtttcg cttacaaaat attgattatg attaaagaca
    14221 aaaagaagat actctttatt ctcctgtatt tgtttttgag tttttctgtc ggttcacagc
    14281 ctgctgttcc cacccatgga caggagcacg aactgacatt atatattatg cccacacttg
    14341 aacctctcga ctggaccagt cctaaaagta tttacacaag cacctttcac tgttatttaa
    14401 aaaatatcac caccaaagac aattacctgt tggggcacgt ggccatcaaa ctgaagtctt
    14461 cactgttgga taaaccactg ctgactactc aaacatcttc ggggatgcag gagaaattag
    14521 atcttatttt caaacaaaaa attggtcttg gtatattggg tgctcccatt acaggtagaa
    14581 tggaaccctc cacggaatta agcaggaaat tgaaagtgta ttccgagcga aacaaactga
    14641 cttttatacg ctacaatata aatgaaaaag cagcccgacg aataatcgaa ttcgtagagc
    14701 agtacaccca ggtgcgcgat tcgtcttttg ccacgtgcaa ctattacggc ggttcgttct
    14761 ggccactcaa caaaaatgaa ggtgccgggt gttcgtcttt tggtattgca gtgctccagc
    14821 tggccgggat agaaccaata gaacctgaaa aatggatagt tgaagtgaaa attcccatga
    14881 agctgatagg gggaagtttg aacaacaaca aaaaaataaa aattaacgaa ataaagaaaa
    14941 ccaatcaatg gtatgagggc aggggggtag ccaatgttga ttacgtaaat tataaggtat
    15001 atgatccgag tcttatgttt gactgggttc ttaacatgac ccggcaaccc tcctcacact
    15061 accgggttat cagcgatgaa ggtactaagg gcttgctgat agatgccaca gcagccgtag
    15121 tggatgaaaa tgagcccatg ttttcaccca acaaggaagc cgacctgttt gtaactcatt
    15181 atctgcaaaa aattccccga caactccaga caaatgaagg gcaggatacg actcagtcgt
    15241 ccgggatacc cgaacatctt tttctgcaat atccaccacg caactaatac ctgccgccct
    15301 taaccccttg cattctgttt taaagaatgt atatcattca ttcgataaaa aaccaatttt
    15361 cattttgcta tttaaaaatg attctctatc tttgcagtcc aaatttgaaa taacaattat
    15421 aacatattaa ataagaaata agtatgatcg tagtacctgt aaaagaaggc gaaaacattg
    15481 aaaaagcact gaaaaaattc aaacgtaaat tcgaaaaaac cggtgttgtt aaagaattac
    15541 gtcgtcgtca atgctttgat aagccttcgg tagtaaaacg cgaaaagaaa atgcacgcgg
    15601 tttacgtaca acacatggat agaaacgaag attaattaaa aaacttcttc gaaaagttgg
    15661 tattctgaca ttattgttct attttcgttg ttaaattctt tcaacgagtt tgtcaggtat
    15721 gatcaacaaa tttctgcaat atttacagta tgagaagaat tattcatctc atactgtttt
    15781 gtcatatcag accgacctcc tgcagttttg cgatttcctc agtatcgact cccttgattt
    15841 ttcccctgcc ggtgtaactc cgctggaaat ccgtcaatgg gtgatgcatc tgattaataa
    15901 taactacacc gcaaaatctg tttcacgtaa aatatctgcc ctgaaatctt tctggcgctt
    15961 cctgctgata cacggactgg ccgaaaaaaa tcccacatta aaaattgttt taccaaaaac
    16021 aaaaaagctg cttcctgcgt tctataaaca acaggagatg gaacgtgcat tggctgaaga
    16081 taccttgccg ggagggtttg agtccttgcg caaccgattg ataatttcat tgctttacga
    16141 agccggattg agggtctcgg aactgacatc acttaccgat gaggattttg acttcgcatc
    16201 gcaacaaata aaagtaatcg ggaaaagaaa caaacaacgg attatacctt taggtggtga
    16261 catctgtgag atgattagcg agtataaaca gttgagaaat aaagaagtgg aacgacaaaa
    16321 caatgcacta ttcctgctta gcagtggttt gcctatgtat tccaaagctg tatataatat
    16381 tgttcacagc gaaatgagcc aggttagtac ccttcacaaa caaagtccgc acgtactgcg
    16441 gcatactttt gctactacat tgttgaataa tggagccgat attaatgcgg tgaaagattt
    16501 gctcgggcat agcagccttg ctgcaaccca ggtctatacc cacgttacat tcagcgagct
    16561 aaataaaatc tataaacaag cccacccacg ggcacaaaaa aaaggaggta acttatgaag
    16621 gtcatgattc aatccatcaa ttttgatgca acagaaaaac tggaagagta tgtaaacaaa
    16681 aaagccaaaa aaatagaaaa attttttgac gaaatcagaa taatcgatgt ttacctgaaa
    16741 gttattaagc cggaaaccgc tacaaataaa caggcagaaa tcaaagtgac ggctcccaat
    16801 gttgaatttt ttgcatcaaa gacctgtgac acatttgagg aagctgtgga tctggcattg
    16861 gaagccatcg aaaagcaagt gattaagttc aaggaaaaaa tagctaaaaa ataaattttg
    16921 gtaaatatat tttgtatatc aaattaattt cgtatttttg caagccgttt gaatagcgat
    16981 atttaaacgg ctttttagcc ctcaaaatgg cataaaatgc actttgcctc tttagctcag
    17041 ctggccagag cacgtgattt gtaatctcgg ggtcgttggt tcgaatccga caagaggctc
    17101 aaattgaccc caaacccctt aaacagggat caaaacaggg cgaaacaaga ttgagaaact
    17161 tcttctactt aaaaagaaaa agagaattaa tcaaatcatt cacttcccta cagaggttgg
    17221 ggagaaaata aaaaaaacat tgggcagtta ccagagtggc caaatgggac tgactgtaac
    17281 tcagttgtct ttcgacttcg gaggttcgaa tccttcactg cccacaagcc taccccaacc
    17341 ctcccaaagg gagggagact ttcccccttc gggggaataa gaagggggct ttatattgcg
    17401 gaagtagctc agttgataga gcattagcct tccaagctga gggtcgcggg tttgagcccc
    17461 gtcttccgct ctcaaaaaaa aagaagtcca ccccaaccca cccaaagtga ggttgacttt
    17521 ctcccgttga gaaacgttga gaaaattata attcctggga taggaaatag aatggtggca
    17581 gcctgctgtt gtagctcagt ggtagagcac ttccttggta aggaagaggt catgggtt
//
LOCUS       DAOPZK010000036        17192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3509_length_17192_cov_23.288090, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000036 DAOPZK010000000
VERSION     DAOPZK010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_17192_cov_23.288090"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..279
                     /locus_tag="PKH68_03690"
     CDS             103..279
                     /locus_tag="PKH68_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12071.1"
                     /translation="MEIPADDAGLSAIKEHIRKDGGKTFTLKGLRTNVKALRSDETTL
                     SNIDKLFETYEKVK"
     gene            complement(725..2047)
                     /locus_tag="PKH68_03695"
     CDS             complement(725..2047)
                     /locus_tag="PKH68_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNX12072.1"
                     /translation="MYSNKKIYQVTYPIFLTLIAQNIVNVTDTAFLGRVSEVALGASA
                     IGGVFYIAIYFVGFGFSQGAQILIARRNGEKKYAEIGPIFNNALIFNFLIATLIFMLS
                     VLFIPDLMRYLVHSDHIYEASVEFLNWRIYGFFFSFLNVVFRAFFVGITRTRVLTISA
                     VITAATNIILDYLLIFGHFGFPQWGIAGAAIASTAAEATTLIYLIAVTLKQDDKHIFR
                     LFKLNKIDFQTIGKILNLSIFIMFQYFISISTWFMFFIFIERLGERPLAVTNIGRSLY
                     ILLMIPGSALSTTVNTLVSNMIGEGHKERVIPFMIKILKIVLLILLPLMVFTFAFPEL
                     FARIYTNNPELVAATIPTLRVVSVAMVFCGLGSILFSVISGTGNTKSAFIIEFITLFF
                     YIVYVYYTAVILRTPVEIVWMSEFVYWTIIGLMAYWYLLKGNWRKKEI"
     gene            complement(2137..3435)
                     /gene="nhaD"
                     /locus_tag="PKH68_03700"
     CDS             complement(2137..3435)
                     /gene="nhaD"
                     /locus_tag="PKH68_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNX12073.1"
                     /translation="MIALIIAVFVVGYAAIAFEHNIKINKTASALITGVICWTIYILF
                     NADTHLVNEELLLHLGDISSILFFLMGAMTIVELIDSYNGFEVITSKITQTNKKSLLW
                     IIGILTFFMSAILDNLTTTIVMVSLLRKLIKDHDTRLFYVGIVVIAANAGGAWTPIGD
                     VTTTMLWIGGQISAFNIMKGLFLPSIISLAVPLIVLSFTMKGDVKRPVVATETYKMLS
                     RNQQVFVLILGVSMLIMVPIFKTLTHLPPFMGMMLGVGIMWIATEIMHRHHTGKDEEQ
                     NRRTLVSVLTRIDLPSILFFLGILLAIAGLEVSGVLGNLAVYMDKTIGNLNAIVISIG
                     LASAVIDNVPLVAATQGMYSMAQYPMDHYLWEFLAYCAGTGGSILIIGSAAGVAAMGM
                     EKINFFWYIKKISWLALIGYLAGAAFYMLEYYFSHGSFYH"
     gene            complement(3594..5381)
                     /gene="lepA"
                     /locus_tag="PKH68_03705"
     CDS             complement(3594..5381)
                     /gene="lepA"
                     /locus_tag="PKH68_03705"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNX12074.1"
                     /translation="MKKIRNFCIIAHIDHGKSTLADRLLENTQTVIGKEMQSQVLDNM
                     DLERERGITIKSHAIQMNYTHGGEEYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ATQGIQAQTISNLYMAIEHDLEIIPVMNKMDMDSAMPEEVEDQIVELLGCKRDDVLRA
                     SGKTGMGIDEILAAIVERIPAPKGDPEAPLQALIFDSVFNSFRGIIAYMKIVNGTIKK
                     GDLVKFVNTGKEYNADDIGILKLDMSPTDTLSAGNVGYIISGIKTSKEVKVGDTITHV
                     KRPCDKAISGFEEVKPMVFAGVYPIDAEDFEDLRSSLEKLQLNDASLTFEPESSVALG
                     FGFRCGFLGLLHMEIIQERLDREFDMNVITTVPNVSYKVHTKKGDLVEVHNPAGLPDI
                     MAIETIEEPYIRASVITRTDYIGQIMTLCLGKRGELVKQEYVSGNRIEIIYDLPLGEI
                     VFDFYDKLKSISKGYASFDYHMNGWRPSKLVKLDILLNGEPVDALSSLIHFDNATSLG
                     RRMCEKLSELIPRQQFDIAVQAAIGAKIIARETIKAVRKDVTAKCYGGDVTRKRKLLE
                     KQKKGKKRMKQIGNVQVPQKAFLAVLKLD"
     gene            complement(5629..5802)
                     /locus_tag="PKH68_03710"
     CDS             complement(5629..5802)
                     /locus_tag="PKH68_03710"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HNX12075.1"
                     /translation="MSPDFYKALEISGIGIAGVFIFMTIFFFVLIGLDKAFPNEKKPK
                     KAAHPADENSGDE"
     gene            complement(5816..6967)
                     /locus_tag="PKH68_03715"
     CDS             complement(5816..6967)
                     /locus_tag="PKH68_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNX12076.1"
                     /translation="MELSQLFPAIAGMTWQNLIMIAVGFTLIFLAIKKQYEPTLLLPM
                     GFGAILVNIPFTAALTQIDPATGQALEGALDVFFKAGIATEVFPLLIFVAIGAMIDFS
                     PLFQRPSLLLFGAAAQFGIFVTMVFATAVGFTLKEAASIGIIGAADGPTSIIVSSILA
                     PNLLGPISVAAYSYMALVPIIQPPVVRLLTSRKERMMRMRSDANKKVSKTALILFPIL
                     VTVLAGLIAPSSLALIGFLMFGNLIRECGVLNSLSKTAQNELANLVTILLGITIASTM
                     TGDRFVTFDTIKILSLGIFAFVFDTAGGVLFAKAMNLFLKDEKKINPMVGACGISAFP
                     MSARVIHGMGLKEDPHNYLLMPAISANVGGQIGSVIVGGLILMLVPLFA"
     gene            complement(7089..8012)
                     /locus_tag="PKH68_03720"
     CDS             complement(7089..8012)
                     /locus_tag="PKH68_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNX12077.1"
                     /translation="MTKSNLSPDTDEKSAKNLSPVVDWIVRLVKGVIVGIGFILPGLS
                     GGVMAVIFGIYDRLLKFLANLKKDFVKNFWFFLPVGIGLLIGIFLFSIVVEKAFGKYV
                     AQFICLFVGFVVGTFPSLYKTAGKQGRKSKDFIIMFASTVLIFALMMMGEQQLMNIAP
                     SIPVWFGSGALIGLGLIVPGMSPSNFLIYFGLYDKMASGIKNGDMATIIPLAVGAIIC
                     VLLFAKLAAYLFRKYYSGMYHFILGMVIGSSLAIFPTIVFPAFNTEGLEKSGLSFAGA
                     FLFCLILFAVGTIASYLFSKVEEKYPREDIF"
     gene            complement(8041..9105)
                     /locus_tag="PKH68_03725"
     CDS             complement(8041..9105)
                     /locus_tag="PKH68_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNX12078.1"
                     /translation="MGSLTLWWVAFIAIFIIVYAIDLAVTARRKETESISTALKWTGV
                     WISIALLFTVAIYFFFPQNPDSTVSTAHVMGTKFLSGYLTEYSLSVDNLFVFIMIFSM
                     MGISEKNQPKLLMIGIMISIVLRILFILVGMELVERFEWIIYVFGAILLYTAYKMVFS
                     KEEDNVDPKSNILYKGASRLFPVDPDVHNPKFFATLNGKKYITVSLLCLLVIGSTDVL
                     FAVDSIPAIIGVIKEGATNILTADEENFLAISSNVFAVMGLISLFFALRGIMGMFRFL
                     KVGVSFILFFIGCKMLLGAIPAVSGFFSAHSWVSLGVIVFTLAFSIVLSMLISENKEV
                     AELKEELKVEKLKDERQDEI"
     gene            9448..12279
                     /gene="uvrA"
                     /locus_tag="PKH68_03730"
     CDS             9448..12279
                     /gene="uvrA"
                     /locus_tag="PKH68_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX12079.1"
                     /translation="MSKQGNILVKGARVHNLKNIDVEIPRNKLVVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSSYARMFLGRMSKPEVDYIKGIPPAIAIEQKVNTRNPRSTVG
                     TSTEIYDYIKLLYARIGRTYSPVSGELVRKHEADDVVQAALEYPDGTRLMVLAPVGPK
                     EGISSEERLNLFLQNGFSRIVIDGEILRISDLLANNQKPDTSKDIYLLIDRLEVDHSA
                     SGISRLTDSVETAFLEGSEACTLKILTENETITSHFSKAFEADGIQFELPNENMFSFN
                     NPLGACPVCEGFGKIIGIDEDLVVPNKSLSIYEDAVVCWRGEVMSEYKKQLIRTADQF
                     NFPIHKPFIELTDEQRELLWTGNSYFEGLNAFFNMLEANQYKIQYRVMLARYRGKTIC
                     PACKGTRLKKEVNYVKINNVPITELVQLPVRRLHEFFETLDLDEHDASVAKRLLIEIK
                     NRIRFLMDVGLDYLTLNRLSNSLSGGESQRINLVTSLGSSLVGSLYILDEPSIGLHSR
                     DTHLLIKVLHQLRDLGNTVVVVEHDEEIMRAADYIIDIGPEAGRKGGNVVWQGKPEMQ
                     PPKAPQSPLPLRDFPLRGLSYTLDYLHGLEKIEVPLHRRKWNNYIEVLGARENNLKGI
                     NVKFPLGIMTVVTGVSGSGKSSLVKTVFYSALKKYYGGVAEKTGQFGSLRGSMHLAKD
                     IEFVDQNPIGRSSRSNPVTYIKAYDEIRKLFADQQLSKQMGYQSSHFSFNTPGGRCEE
                     CQGEGTVTVEMQFMADLVLECEHCHGKRFKQDILDVSFKEKNIYDVLDMTVNQAIEFF
                     SEHTGSTEKRIVKRLLPLQNVGIGYVKLGQSSSTLSGGESQRVKLASFLANEKPEPTI
                     FVFDEPTTGLHFHDIKTLLKALNALIAKGHTVIIIEHNLEVIKCADHVIDIGPEGGEN
                     GGNVVFEGTPEELVSCECSYTGKFLKNKL"
     gene            12339..13538
                     /locus_tag="PKH68_03735"
     CDS             12339..13538
                     /locus_tag="PKH68_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12080.1"
                     /translation="MKRILLGVGILLFTAVLNAQDIAKTITDYEETKSTIISKGRKLL
                     LDQFVADRIDKVEEVYTYLNDKVADIDYAAFNAFEQINLSCWTRRYEYALKEILYLDS
                     LSTARKDVQSSGPSRIYPENDRLSTRIYEKTAPVLSSVLNNIETSELSREQKDVLQLI
                     MKDIYLTGNNRSKSPFQDSINVLSTKFLSSYPASPYANYIRNSVRFVYHRNDWGFGYD
                     FGLGYTQVMGSTADNYKNGFNANGGIDIYYKQTVAYIRFLMSTSGTKHSVVANQGNVI
                     PPETFMNFNHFEFSLGYALPDLKLLTVTPFAGIGAFGISYGTAEERTAGSMLKNAGKT
                     VLCYQGGLNLDFNLTKKNTTEGKIPLRLRCTYIKPANMIYKDMNTEVLTLSLGLSLQG
                     WGTKREL"
     gene            complement(13617..16445)
                     /locus_tag="PKH68_03740"
     CDS             complement(13617..16445)
                     /locus_tag="PKH68_03740"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNX12081.1"
                     /translation="MKLNSLKLTILCLLLIMTAGMFAQQPQMQPLPMDPKVRYGTLEN
                     GLTYYIRANKEPKERAEFHIAQNVGAILENDDQNGLAHFLEHMAFNGTKNFPGKGIIN
                     YFEKNGVKFGYDINAYTSLDETVYRLSNVPTAREGLLDSALLVLHDWSGFILLQENEI
                     DSERGVIREEWRTGNNASRRMWKTSNAKKYPGSQYAKRDVIGDTAIINNFTYKTLRDY
                     YKKWYRPDQQAIVVVGDVDVDKMEAKIKQIFSDIPKKENFGERPVYEIADNKAPIVAI
                     VTDPEATQTQMEVEYKKDKLPKEVSLAMPGYVMNLANSLISSVLSERFEEITMQADAP
                     FAAAMAGYSELVKSKDAFQMVVVPKEGKELEGLNALLLEAEKMKRFGFTNAELERAKT
                     NMLKNFEKSYNERDNQKSISLAREYIRHYLSNEPIPGIEFEYQTVQMILPQLKTEMIN
                     QLAKSYVSDTNVVISVMAPEKANVKVPTESQILAAIDATQKAELTAKAEEVMNKPLLE
                     KTPKAGKVKKIKQNPAMETTEMTLSNGVKLVFKPTTFKKDEITMRAFSDGGLSKVHNV
                     ADLPSASMATGIVQANGIGNYTAVELGKILTGKIASVSPSIGQYEEGMSGNSSVKDFE
                     TMLQLTYLYFTQPRKDDNSYAALINMYRTQLANVASNPNKAFYDSIQTTTTANDPRTV
                     LQSLKTLEKVNQDKALEIYKQRFANPADFTFIFVGNIDPNNKEIQQLLATYLGGLKTN
                     KKNVEKFDKVYREFPKGKVNNYFTFNMKTKKASNRIIYTADMPYNINNEVTVSAIGDI
                     LDMRYLESIREKEGGSYGVGVYGGLNNVPKDQATLLMQFDTDPEKQAKLMGIIHEEVQ
                     QIVNNGPLKDDLAKVKENLMKQYKQDLEQNSWWSSSLKTFYQDGLNYRTDYKAAVDAL
                     TPDAIQKTLKAIVDQKNVIEVVMKPAAE"
     gene            16616..17101
                     /locus_tag="PKH68_03745"
     CDS             16616..17101
                     /locus_tag="PKH68_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNX12082.1"
                     /translation="MSENLILSANADKETRYHEIIPQLKALIEGETDMTANLANVSAV
                     LKEAFGWWWVGFYLVKGEQLVLGPFQGPVACTRIAFGKGVCGTAWKKKKSILVPDVEQ
                     FAGHIACSSASVAEIVIPLINNQNEVIGVLDIDSEKYDILDETDVKWLEEIVRIVAPA
                     P"
BASE COUNT         5168 a         3682 c         3560 g         4782 t
ORIGIN      
        1 cccggcggac gatgcgggac tttcagcaat taaagagcat atccggaagg acggatttat
       61 aaatccggat taaaaaatcc ttctgtttaa agagccgtga ttatggaaat cccggcggac
      121 gatgcgggac tttcagcaat taaagagcat atccggaagg acggaggaaa aacatttacc
      181 ctaaaaggat taagaaccaa tgtaaaagca ctacgaagcg atgaaaccac tttgtcaaac
      241 attgataaac tatttgaaac ctatgaaaaa gttaagtagt cgtccattaa aaaccacata
      301 aacaaaagaa tatcaacaaa atgcctttca aaatagttgt ataagtctgc aagatttagt
      361 atatttgaag cataaatctt gcaaataaat tatgctatca aagaagaaag acaccacaca
      421 gagcagtatg ttttttggat tgaaagatat gttaaaccaa aaacatccga tttttttgga
      481 aaagagcaaa attttgaagt caacatgact ttttataatg cggtctcaac gaggcattca
      541 ttttgcaacg ccaataatac tcatcgctga tattgttgcc tcaagggggc ttcaaattaa
      601 gcaccggagg acattttggt tttaataaaa aatacagacg aaatctgtca tattattgat
      661 tgtgtagtct atatcatcat gttgaaatta acaataaata aaatttgaag tcaggcaaag
      721 atgttcaaat ttcttttttc ctccagttac ctttcagcag gtaccaatat gccatgagcc
      781 ctataatggt ccagtacaca aattccgaca tccagacaat ttcaaccggg gtgcggagta
      841 ttacagcggt gtaataaaca tatacgatgt agaaaaaaag tgtaatgaat tcgattataa
      901 aagctgattt agtattgcct gtacctgaga taacgctgaa caaaatactg cccaacccgc
      961 aaaaaaccat ggccaccgaa actacccgca atgtgggaat agtggcggct acaagttcag
     1021 gattattggt gtaaatgcgg gcaaaaagtt ccggaaatgc aaacgtgaaa accatcagcg
     1081 gaagcaaaat cagaagaaca atcttcagta tttttatcat gaacggtatg acccgctctt
     1141 tatgcccttc acctatcata ttgcttacca gcgtattgac ggtggtggaa agtgccgatc
     1201 cgggtatcat aagcagaatg taaagacttc ggccgatgtt ggtgactgcc agaggccgct
     1261 caccgagcct ttcgataaaa ataaaaaaca taaaccaggt ggaaatggag ataaaatact
     1321 gaaacatgat aaaaatcgac aggtttaata tctttccaat agtctgaaaa tcaattttat
     1381 tcaatttgaa cagacggaaa atatgtttgt cgtcctgctt cagggtgacg gcgataagat
     1441 aaataagcgt agttgcttcc gctgcggtgg atgcaatggc agcaccggca ataccccatt
     1501 gtggaaagcc gaaatggccg aaaataagca gataatccag tataatattg gttgcagcgg
     1561 taatcacagc ggaaattgta agaacgcgtg tgcgggtaat tcccacaaaa aaagcacgaa
     1621 aaaccacatt gagaaacgaa aagaaaaatc cgtaaatacg ccagttgaga aattccacag
     1681 aagcctcgta aatatgatcg gaatgcacca gatacctcat cagatccgga atgaataata
     1741 ccgaaagcat aaatatcagg gtagctatca ggaaattaaa tatcagggca ttgttgaata
     1801 tcggtcctat ttcagcgtat tttttctctc cattccgccg ggctatcagt atttgcgctc
     1861 cctggctaaa cccaaatcca acaaaataaa tagcgatata aaacacccca ccgatagccg
     1921 aagcacccag cgccacttcg ctcacgcgcc ccagaaaagc cgtatccgtt acattgacaa
     1981 tgttttgcgc tatcagcgta aggaaaattg ggtaggttac ctgatatatt tttttgttgg
     2041 aatacaatga attagctatt atgtgattaa aaagtattct ataatgaaca taaaaaaggc
     2101 tgcttttgtt aacggcagcc ttttgttaca ttttttttaa tgataaaatg agccgtgtga
     2161 aaaatagtat tcaagcatat aaaaagctgc cccggctaag tatcctatca acgccagcca
     2221 gctgattttc ttgatatacc agaagaaatt gattttttcc atacccatgg cagccactcc
     2281 ggcagccgaa ccgataatca ggatacttcc acctgttccg gcacagtaag ccagaaattc
     2341 ccacagatag tgatccatcg gatattgagc catggagtac ataccttgag tggcagctac
     2401 cagcggaaca ttatcaatca cagccgaagc aagcccgata gagatgacaa tagcgttgag
     2461 gttgccgatt gttttgtcca tatatacagc caggtttccg agaacgcccg atacttcgag
     2521 accggcaata gccagcagaa taccgaggaa gaaaaggata cttggaaggt caatgcgggt
     2581 cagcacactc accagagttc ttctgttttg ctcttcgtcc ttaccggtgt gatgacggtg
     2641 cataatttca gtggcaatcc acatgatacc tacccccagc atcatgccca taaatggagg
     2701 caaatgagtc agtgtcttaa atattggcac cattattaac atggatacgc ccaaaatcag
     2761 tacaaatacc tgttggttgc gggaaagcat tttatacgtt tcggttgcca cgacaggtcg
     2821 ttttacatcg cctttcatgg tgaatgacaa cacgataaga ggtacagcta aactgatgat
     2881 gcttgggaga aataaccctt tcatgatgtt gaaagcagaa atttgtcctc caatccacag
     2941 catagtggtt gtcacatcac cgatgggtgt ccaggcacct ccggcattgg cagctatcac
     3001 tactatgccc acgtaaaaca ggcgtgtgtc atgatccttt atcagttttc ggagaagcga
     3061 aaccataacg atggtggtgg tcaggttgtc gagaatggcc gacatgaaga atgtgagaat
     3121 tccgataatc cacagcaagg attttttatt tgtctgggtg attttactcg ttatcacctc
     3181 aaaaccattg tagctgtcta tcagttcaac aatggtcatg gctcccatga ggaaaaacag
     3241 aatgctggat atgtcaccga gatgaagtaa caattcctcg ttgaccaaat gcgtatctgc
     3301 attaaaaagg atgtaaatgg tccagcagat tacgccggta ataagagccg aagctgtttt
     3361 attgattttg atgttgtgct caaaagcaat ggcagcataa ccgactacaa aaactgcaat
     3421 tattagtgct atcatacatt tcaaaaaaaa tgttccaaac cttcagttag aaatttcaga
     3481 acattacgtt aagggttatt tttatattta atattttctg atttatgaaa cggaaattga
     3541 ctacgaaagt tcaaatgagc tgatcaatta cggtaatttc tttgataaaa aaattaatcc
     3601 agtttcagca cagctaggaa tgccttttgt ggaacctgaa cattaccgat ttgcttcatc
     3661 cgtttttttc cttttttctg tttttcgagc aattttcttt tacgggtcac gtcgccgccg
     3721 tagcattttg ctgtcacgtc tttgcgcacc gctttgatgg tttcgcgggc aatgattttg
     3781 gcaccgattg ccgcctgaac agcaatatcg aactgctgac gcggaataag ttcactcagt
     3841 ttctcacaca tgcgacgtcc gagtgaagtg gcattatcaa aatggatgag cgacgataag
     3901 gcatccacgg gctcgccgtt gagcagaata tccagcttca ccagcttcga gggacgccag
     3961 ccgttcatgt gataatcgaa cgaagcgtaa cccttggaaa tgcttttaag tttatcataa
     4021 aaatcaaaaa cgatttcgcc gagcggcagg tcgtaaataa tctcaatccg gttgcccgac
     4081 acatattcct gcttcaccag ttcgccgcgt ttacccagac aaagggtcat tatttggccg
     4141 atgtagtcag tacgcgttat aacagaggct ctgatatacg gttcttcgat ggtttcaata
     4201 gccatgatat cgggcagtcc ggccggattg tgcacttcta cgagatcacc ttttttggta
     4261 tgcactttat aggacacgtt gggcactgta gttatcacat tcatatcaaa ctcccgatcc
     4321 aaccgttcct ggattatctc catgtgaagt aatcccagaa atccgcaacg aaaaccaaag
     4381 cccagcgcta ccgacgattc cggttcaaac gtaagcgatg catcgttcaa ctgcaatttc
     4441 tccagcgacg agcgtaaatc ttcaaaatct tcggcatcga tggggtaaac tcccgcaaaa
     4501 accatcggtt tcacttcctc aaaacccgat atggctttgt cgcacggtcg tttcacgtgg
     4561 gtaatggtat ctcccacttt cacttctttg gatgttttga tacccgaaat gatgtaaccc
     4621 acattgccgg cactcagtgt atcggtgggc gacatgtcca gtttcaaaat gccaatgtca
     4681 tctgcgttgt actcttttcc ggtattgaca aatttaacca gatcgccctt ttttatcgtt
     4741 ccgtttacta ttttcatgta ggcaataatg ccccggaatg aattgaatac cgaatcaaaa
     4801 atcaatgcct gaagcggggc ttcgggatct ccttttggag ccggaattcg ttcgacaata
     4861 gcagccagta tttcatcaat tcccatccct gttttgccac tggcacgcaa tacgtcatcc
     4921 cgtttacatc cgagtaattc tactatttgg tcttccactt cctcgggcat ggcgctgtcc
     4981 atatccattt tattcatcac cggaattatt tccagatcgt gttcaatggc catatacagg
     5041 ttggaaatag tctgtgcctg aataccctgc gtggcatcta caatgagcaa tgctccttcg
     5101 caggcagcta tagatcggga gacttcgtac gaaaaatcca cgtgtcccgg agtatcaatc
     5161 agattgaggg tatattcttc gccaccgtgg gtataattca tctggatggc gtgacttttg
     5221 atggtaatgc cacgctcccg ttcaagatcc atattgtcca gcacctgcga ctgcatttcc
     5281 ttaccgataa cggtctgcgt attttcaagc aggcgatcgg caagagtact tttaccgtgg
     5341 tcaatatggg caataataca aaaatttcgg atcttcttca tctgtaatta actgtcaatt
     5401 ttatttacgg gtacaaaaat acgaaaaaaa actcatctct gcgattgttg actgctccgg
     5461 gatgacaatt ttatttgacc taaacatatt aatatcaacc caaaaaccga aaaatatgtt
     5521 ttgataccat tgacccggtc aatctcttaa cctcctgtga attaaaaagc cggaaacatt
     5581 atttcttgaa ataacatttc cggcttattt tacagtttga atacctgttt attcatcccc
     5641 cgaattttcg tcggcaggat gagctgcttt tttgggtttc ttttcgttag gaaatgcttt
     5701 atcaagtcct atgagaacaa aaaagaaaat tgtcataaaa ataaatacac cggcaatgcc
     5761 aattccggaa atttctaagg ctttataaaa atctggactc ataattctac gttttttaag
     5821 caaataatgg aactaacatt aaaataagac cacctacgat tactgaacca atctgtccgc
     5881 ctacattggc gctgattgcc ggcatcagca ggtaattgtg cggatcttcc ttgagaccca
     5941 ttccatgaat tacacgggca gacatgggga atgctgaaat gccgcacgca ccaaccatcg
     6001 ggttgatttt cttctcatcc ttcaggaata aattcatcgc cttggcaaac aatacgccac
     6061 cggctgtgtc aaatacgaac gcaaaaatac ccaaactaag tattttgatg gtatcaaaag
     6121 ttacaaaacg atcgccggtc atggtgctgg ctatggtaat accaagcaat atagttacca
     6181 ggttcgcaag ttcattctgt gctgttttgg acaaactgtt aagtacaccg cactcgcgga
     6241 tgaggttacc gaacatcagg aagccgataa gagcgagcga agagggcgct attagacctg
     6301 caagcactgt aacgaggatg ggaaacagga ttaaggctgt tttcgacact ttcttgttgg
     6361 catcagaacg catacgcatc attctttctt tgcgggaggt aagcaatcta actaccgggg
     6421 gctgaataat aggaaccagt gccatatacg aataggctgc tacagaaata ggtcccaata
     6481 aattaggagc aaggatggaa gatacaatga tggaggtggg accgtcggca gcaccaataa
     6541 ttccgatgga tgcagcttct ttaagcgtga aacctacagc agtggcaaac accatcgtaa
     6601 caaaaatacc aaactgagca gcagctccga acagcaacag tgaaggacgc tgaaaaagcg
     6661 gtgaaaaatc aatcattgca ccgatagcca caaaaataag aagtggaaac acttcggtgg
     6721 cgatacccgc tttgaagaat acgtccagtg ctccttccag ggcttgcccg gttgccgggt
     6781 caatttgtgt aagtgctgct gtaaaaggga tattcaccag tatggcacca aatcccattg
     6841 gcagcaggag ggttggctcg tactgcttct taatggctaa aaaaatcaat gtaaacccga
     6901 cggcaatcat aatgagattt tgccaggtca ttcccgcaat ggcgggaaat agttgcgata
     6961 attccatggt ttgtagttta attaaatagt gatctgttat aattgtgttt taaataaata
     7021 ctgtttttgc agcttaattt tttatgaaaa gttctgtacg attaacgaag tattctaaat
     7081 attatatgct aaaatatatc ttcacggggg tatttttctt ccaccttgct gaaaaggtaa
     7141 gaagctattg tccccacagc aaatagtatc aaacaaaaaa ggaatgctcc ggcaaaactt
     7201 aaaccggact tttcaagacc ttctgtgtta aatgcgggaa aaacaattgt tggaaaaatg
     7261 gccaatgatg acccgatgac catcccaagt ataaagtggt acattcccga ataatacttg
     7321 cggaataaat aagccgccaa tttggcaaat aaaagcacgc aaattattgc tccaacagcc
     7381 aatgggataa ttgtagccat atctccattt ttaataccgg aagccatttt gtcgtatagc
     7441 ccgaaataaa tcagaaaatt agaagggctc atcccgggta caataagccc aagaccaatg
     7501 agagcgcctg aaccaaacca gacgggaata ctcggcgcaa tgttcatcag ctgttgctct
     7561 cccatcatca tcagagcaaa aatcaagact gtagaagcaa acataatgat aaagtccttt
     7621 gactttcgac cctgcttccc ggcagttttg tacagcgaag gaaaagtacc taccacaaac
     7681 ccaacaaaaa ggcaaataaa ttgcgccaca tatttcccga aagctttttc tactacaatc
     7741 gaaaaaagaa aaatgccaat taaaagccca atgccaacag gaagaaaaaa ccagaaattc
     7801 tttacaaaat cttttttgag atttgccaga aatttcaata agcggtcgta aatgccgaat
     7861 attacagcca tcacacctcc cgacaatccg ggaagaataa aacctatccc cacaattaca
     7921 ccttttacca gtcttactat ccagtcaacg acaggactta ggttctttgc tgatttttcg
     7981 tcggtatctg gtgataaatt actttttgtc attagcttat tttgtatttt ttttctacaa
     8041 tcaaatttca tcctgtcttt catccttcag cttctccact ttcagctcct ctttcagttc
     8101 cgcaacttcc ttgttttcag aaataagcat ggaaagtacg atggagaatg ccagggtaaa
     8161 aacaattaca cccagcgaaa cccacgaatg agcggagaag aaacctgaaa ctgccggaat
     8221 ggcacccagc aacattttac atccgatgaa gaataaaatg aaacttacgc ccactttgag
     8281 gaagcggaac atacccatga tgcctctaag cgcaaaaaac aaggatatca accccatcac
     8341 agcaaacaca ttggaactga tagccaggaa gttttcttca tcggctgtca gaatattggt
     8401 ggctccttcc ttgattacac cgataatggc aggtatggaa tcaacagcaa ataaaacatc
     8461 ggttgaaccg attacaagta aacagagcag gcttacggtg atatattttt tcccgttaag
     8521 ggtagcgaaa aatttcggat tgtgcacatc aggatcaacc gggaaaagtc tggaagcacc
     8581 tttatagaga atatttgact tgggatccac attgtcttct tccttggaga aaaccatttt
     8641 ataagccgta taaagcagaa tagcaccaaa aacataaata atccattcaa atctctccac
     8701 cagttccatc cccacaagaa taaacaatat gcgcaatacg atggagatca taataccgat
     8761 catcaggagc tttggttgat ttttctcact aattcccatc atggagaata tcatgataaa
     8821 aacaaaaagg ttatcaactg aaagagaata ctcagtcaga taccccgaaa ggaattttgt
     8881 acccatcaca tgggccgtac tgacggtact gtcgggattt tgcggaaaga aaaaataaat
     8941 tgccacagta aaaagcaagg caatactaat ccagacgccg gtccatttta aagctgttga
     9001 aatactttct gtttctttac gtcgtgctgt cacggccaaa tcaatagcat aaactataat
     9061 aaagattgcg ataaaagcaa cccaccacaa ggttagtgaa cccatacaat taaaattttt
     9121 tatgtgtgat taattaataa attcgaaaca attaagtctt ttatctcaat aaaatccgaa
     9181 aaaaagcact ataaagtaat tctacagctt tgacaaacaa cacattaaca atattatgcc
     9241 atttttggaa atccgaacgc taaggtattg aataatttcg tcatattaat gataatttta
     9301 aagtttcttt atcaaaaaga aatatatcaa aatcgtatgg atgaaatgaa tttatactat
     9361 gaaagaagta ttatgagtat tcgctgaaaa gtagaatatt gcttaccttt gcagattgca
     9421 attctctcta aaaaatacgc cgttagtatg tcaaaacaag gaaatatact ggtaaaagga
     9481 gcccgggttc acaacctgaa aaatattgat gtagaaattc cacgtaacaa actggtggtt
     9541 atcaccggat tatcgggttc aggaaaatca tcgctggctt ttgacaccct ctatgctgaa
     9601 ggtcagcgcc gctacgtgga aagtctatcg tcctacgcgc gcatgttttt gggtagaatg
     9661 tcgaaacccg aagtggatta catcaaaggt attccaccgg ctattgctat tgagcaaaaa
     9721 gtcaacaccc ggaatccccg ctcgaccgta ggcacttcca ccgaaattta tgattatata
     9781 aaattgctgt atgcccgtat cgggaggact tattcgcctg tttcgggtga actggtcagg
     9841 aaacatgaag ccgacgatgt ggttcaagct gcactcgaat accccgacgg taccagactg
     9901 atggtattgg ctccggtagg accaaaagaa gggataagca gcgaagaacg cctgaacctg
     9961 tttctgcaaa acggtttcag ccgcattgtt attgacggtg aaattcttcg aatatcggat
    10021 ctgttagcaa ataaccagaa acctgatact tcgaaggata tctacctgtt gattgaccgg
    10081 ctcgaagtgg atcactctgc gtcgggtata agccgcctga ccgattcggt cgaaacagca
    10141 tttctggaag gaagtgaagc ctgtacgtta aagattctaa cagaaaacga aaccattaca
    10201 tcgcattttt ccaaggcatt tgaagcagac ggcattcagt ttgagctgcc caacgagaat
    10261 atgttcagtt tcaacaatcc gttgggagcc tgcccggtat gtgaaggttt cggaaaaata
    10321 atcgggatag acgaggattt ggtagtaccc aataaatcgc tttccattta cgaggatgca
    10381 gtcgtgtgct ggcgcggtga agtgatgagt gaatataaaa agcagttgat ccggactgcc
    10441 gatcaattta atttccccat tcacaaacca ttcattgaac tgaccgacga acagcgcgaa
    10501 ctgctctgga caggcaacag ctatttcgaa gggctcaatg cttttttcaa catgctggaa
    10561 gccaatcagt acaaaattca ataccgggtg atgcttgccc gataccgcgg aaaaacaatc
    10621 tgtcccgcct gcaagggtac aaggttgaaa aaagaagtga actatgtaaa aataaataat
    10681 gtacccataa ccgaactggt gcagctgccc gtaagacgcc tccacgaatt cttcgaaacg
    10741 ctggacttgg acgaacacga tgcttctgtg gcaaaacgtc tgctgattga aatcaaaaac
    10801 cgtattcgat tcctcatgga tgtgggactg gattatctga ccctcaaccg gctttcaaat
    10861 tccctttcgg gcggtgaaag ccagcgcatt aatcttgtta cttcgctggg aagcagtttg
    10921 gtaggttcac tgtatatatt agacgaaccg tccatcggac ttcattccag agatacacac
    10981 ctgcttatca aagtgcttca tcaattgcgg gatttgggaa atacagtagt ggtggtggaa
    11041 catgacgagg aaataatgcg tgcagccgat tatattattg atatcggacc ggaagcagga
    11101 cgtaaaggag gaaatgtggt gtggcaggga aagcctgaaa tgcagccccc taaagcccct
    11161 caaagccccc ttccccttcg ggacttcccc ctcagggggc tttcctacac ccttgattat
    11221 ctccacggtc tggagaaaat agaagtacca cttcaccgtc gtaaatggaa caactacatt
    11281 gaggtgctcg gagcacgtga gaacaacctg aaaggtatta acgtaaaatt tccgttgggt
    11341 atcatgacgg ttgtgaccgg tgtgagtggt tcgggtaaat cgtcactggt aaaaacggtg
    11401 ttttattcag ctctgaaaaa atactacggc ggtgtggccg aaaaaacggg acaattcggt
    11461 tcgctccgtg gcagcatgca cctggcaaag gatattgaat tcgtggatca gaatccgata
    11521 ggacgctcaa gccgttcgaa ccccgtaacc tacatcaaag cctacgatga aatccgcaaa
    11581 ctttttgccg accagcaact ttccaaacaa atgggatatc agtcttccca cttttcgttc
    11641 aatacaccgg gcggtcgttg tgaagaatgt cagggggaag gtactgtgac tgtagaaatg
    11701 cagtttatgg ccgatctggt actggaatgc gaacattgtc acggcaagcg gttcaaacag
    11761 gatattctgg atgtttcctt taaggaaaaa aacatctacg atgtactgga tatgactgtt
    11821 aaccaggcta ttgaattttt ctcggaacat accggaagta ccgaaaaacg catcgtaaaa
    11881 cgattattgc cgctgcaaaa tgtgggtatt gggtacgtaa aattgggaca atcatccagc
    11941 acactttcgg gcggcgaaag ccagcgtgtg aaactggctt cattcctggc caacgaaaaa
    12001 cccgaaccga ccatttttgt atttgatgaa cccacaacgg gacttcattt tcacgacatc
    12061 aaaaccctgc tcaaagcact gaatgcactg atagccaaag gtcataccgt cattatcatt
    12121 gagcacaacc tggaagtgat taaatgcgcc gatcacgtca tagacatcgg tcccgagggt
    12181 ggagagaatg gtggaaatgt agtgtttgag ggaactcccg aagaactcgt gagctgtgaa
    12241 tgttcttata ccggtaaatt tctgaaaaat aaattataag ttgaatttcg tttgataatt
    12301 tgaatttttg gttaattttg tcaaaaaacc aaatcaatat gaaacgaatt ttgcttggcg
    12361 taggaatttt actttttacc gcagtactaa atgctcagga tattgccaaa accattactg
    12421 attacgaaga gacaaagtca accatcattt caaaaggtcg caaactgttg ctcgatcagt
    12481 ttgtagccga ccgcatcgac aaagtagaag aggtttacac gtatctgaac gataaggttg
    12541 ccgatatcga ttatgctgca ttcaacgcat ttgagcaaat taacctgtcg tgctggacac
    12601 gccgctacga atacgcgctc aaagaaatac tgtatctcga ctcgttaagt acagcccgca
    12661 aagatgttca atcttccggg ccaagcagaa tttatcccga aaatgaccgg ctgagcaccc
    12721 gaatctacga aaaaacagca cccgtgttat cttcggtgct gaataatatt gagacatcgg
    12781 agctgagtcg tgaacaaaaa gatgtgcttc agttaatcat gaaagatatt tatcttaccg
    12841 gtaataatcg cagcaaatca ccttttcagg attcaatcaa tgtgttgagt actaaatttc
    12901 tttccagcta tcctgcttca ccctatgcca attacatccg caattcagta cgctttgttt
    12961 atcaccgcaa tgactggggt tttggatacg atttcggact gggttacacg caggtaatgg
    13021 gctccacggc cgataactac aaaaacggat ttaacgcgaa cggtggaatt gatatttact
    13081 ataaacagac ggtggcttat atccgttttc tgatgagtac ttccggcaca aaacacagcg
    13141 tggtggcaaa tcagggtaat gtcatacctc ccgaaacatt tatgaatttc aatcacttcg
    13201 aattcagtct gggttatgca ttgcccgatc tgaaactcct gaccgtaact ccatttgccg
    13261 gtatcggagc ttttggtata agctacggaa cggctgaaga acgcacggct ggttccatgc
    13321 tgaagaatgc cggaaaaacc gtactttgct atcagggagg attgaacctg gactttaatc
    13381 tgaccaaaaa gaatacaaca gaaggaaaaa taccgctgcg attgcggtgt acgtatatta
    13441 aaccggcaaa tatgatctac aaggatatga ataccgaagt tctcaccctt tcactgggac
    13501 tgtcattgca aggctgggga acaaaaaggg aattgtaaca accatttact ttagaaagaa
    13561 aacagggaaa attcgtcttg aattttccct gttttctttt ttttattctg tccgacttac
    13621 tcagcagccg gtttcataac tacttctatt acatttttct gatctacaat cgctttcagt
    13681 gttttctgaa tagcgtcagg agtcagtgca tcaacagcgg ctttgtagtc ggtacggtaa
    13741 ttcagaccat cctggtagaa tgtcttcagt gaactgctcc accagctgtt ttgttccaaa
    13801 tcctgcttgt attgcttcat cagattttct ttcactttgg caagatcatc tttgagcgga
    13861 ccgttgttga caatctgctg aacttcttcg tgtatgatac ccatcagttt agcctgcttt
    13921 tcgggatcgg tgtcaaactg catcagaagg gttgcctgat ctttcggtac attgtttaag
    13981 ccaccataaa ctcccacacc gtacgatccg ccttcttttt cgcgaatgct ttccaggtag
    14041 cgcatgtcca gaatatcgcc aatagccgat acggtaactt cgttgttgat gttgtatggc
    14101 atatcagctg tgtaaataat tcggtttgat gcctttttag tcttcatatt gaaggtgaaa
    14161 tagttattta cctttccttt ggggaattcg cggtaaactt tatcgaattt ttccacattt
    14221 ttcttattgg ttttcagacc tccaagataa gttgcgagta attgttggat ttccttgtta
    14281 ttcgggtcaa tattacctac aaatataaac gtaaagtctg cgggatttgc aaatcgttgt
    14341 ttgtagattt ccagcgcctt atcctggttc actttttcga gtgttttcaa tgattggaga
    14401 accgttctcg gatcgttagc tgttgttgtc gtctgaatgc tgtcgtagaa agctttattc
    14461 ggattgctgg caacattggc caactgagtg cgatacatat tgatgagcgc tgcatacgaa
    14521 ttatcatcct tgcgtggctg cgtaaaatac agataagtca gctgcagcat cgtttcaaaa
    14581 tccttaaccg atgaatttcc actcattcct tcttcatact gtccgataga aggtgaaaca
    14641 gaagcaatct taccggtgag tatttttcct aattccactg ctgtatagtt gccaatgccg
    14701 ttggcctgaa caatacctgt agccatactt gccgaaggaa gatcggccac attgtgaact
    14761 ttggataaac cgccatcact gaacgctctc atggttattt catccttttt gaaggtcgtt
    14821 ggcttgaaaa ccagtttcac accattgctc agggtcattt ctgttgtttc catggcaggg
    14881 ttctgcttga ttttctttac tttccctgct tttggggttt tttcaagaag cggtttattc
    14941 attacttctt cagctttggc agtcaattcg gctttttgcg tcgcatcgat ggctgcaagt
    15001 atctgacttt cagtcggcac tttcacattc gctttttccg gagccattac cgaaataacc
    15061 acattggtat cagacacata cgatttggcc agttggttaa tcatttcggt tttcaactgt
    15121 ggcagtatca tctgtacggt ctgatattcg aactctatac ccggaatggg ttcattgctc
    15181 aggtagtggc gtatatattc acgggccaga ctgatactct tctgattgtc gcgttcgttg
    15241 tatgatttct caaaattttt caacatattg gttttcgctc tttcgagttc ggcattggta
    15301 aaaccaaacc gtttcatctt ttctgcttca agaagtaaag cattcaaacc ttccaattcc
    15361 ttaccttctt tgggaaccac aaccatctgg aaagcatcct ttgattttac aagttcgcta
    15421 tagcctgcca ttgcagctgc aaatggagca tcagcctgca tggtgatttc ttcaaatcgt
    15481 tcgctaagaa cggaagatat cagtgaattc gccaggttca taacataccc gggcatggcc
    15541 aaactcacct ctttgggcag cttatccttt ttgtattcca cttccatctg tgtctgtgta
    15601 gcttcaggat ctgttactat tgcaacaatt ggtgctttat tatctgcaat ttcataaaca
    15661 ggacgttcgc caaagttttc ttttttcgga atatcagaaa atatttgctt gattttggct
    15721 tccattttat ccacatccac gtcgccgaca acaacaatgg cctgctgatc cggacgatac
    15781 cattttttat aataatcgcg cagtgtttta tatgtaaaat tgtttataat ggctgtatcg
    15841 cctattacgt cgcgtttagc atactgcgat ccgggatatt ttttagcatt ggaagttttc
    15901 cacatacgtc tcgaagcatt gttaccggtg cgccactctt cgcggattac gccacgctcg
    15961 ctgtcaatct cattttcctg gagaagtata aaaccagacc agtcgtgcaa cacaagcaga
    16021 gcgctgtcca gcagtccttc gcgggctgtt ggaacattag ataagcgata cacagtttcg
    16081 tcaagcgaag tgtaggcatt aatatcatac ccgaatttta ctccgttttt ttcaaaataa
    16141 ttgataatgc ctttcccggg aaagttcttt gttccattga aagccatgtg ttccagaaaa
    16201 tgtgccagac cattctggtc gtcattctca aggatagcac ccacattttg ggcaatgtga
    16261 aattcagctc tttcttttgg ttccttattg gcacgaatgt aataagtcaa accattttcg
    16321 agtgttccgt aacgaacctt cggatccatt ggcagcggtt gcatctgagg ttgttgtgca
    16381 aacatgcctg ccgtcatgat caacagcaaa cataagattg ttaatttcaa tgaattaagt
    16441 ttcataagat tttttaattt ttacttttaa tttacaaaca atacacaaag gaaacttttt
    16501 tttaacaaac taccaattaa tatcaaacaa ttagcgttaa tttaattgaa aaagctttat
    16561 agcaggcttt tcggataaag ttttgtattt ttgcataaac gaaaaaatac aaactatgag
    16621 cgaaaatctt atcctgtcgg ccaacgccga taaagaaaca cgataccatg aaattattcc
    16681 tcaactgaaa gcactgatag aaggggaaac tgacatgact gctaatctgg cgaatgtatc
    16741 tgcggtactg aaagaggctt ttggctggtg gtgggtaggt ttttacctgg tgaaaggcga
    16801 acaactggtg ttgggtcctt ttcagggacc ggtggcttgt acgcgtattg cattcggaaa
    16861 aggtgtgtgc ggtacagcgt ggaaaaagaa aaaatctata ctcgttccgg atgttgagca
    16921 atttgccggg catatagcct gcagcagtgc ttcggtggcg gaaatagtta tacccttgat
    16981 aaataatcaa aacgaggtta tcggagtgct tgatattgat agtgagaaat atgatatact
    17041 ggacgaaacg gatgtgaaat ggctggaaga aatagtgaga atagtagccc cggcccccta
    17101 accctcccga taaatcggga gggttagggg gctgaccttt catttacaga tcacgcaccc
    17161 ttcacattta ctcagttcgc cgcgtaatcg tt
//
LOCUS       DAOPZK010000037        16848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3612_length_16848_cov_25.204133, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000037 DAOPZK010000000
VERSION     DAOPZK010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16848
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3612_length_16848_cov_25.204133"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKH68_03750"
     CDS             <1..428
                     /locus_tag="PKH68_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNX12083.1"
                     /translation="SVANSSREYRKVYKVIDGDTFWMKDGFGQIEKIRLTGIDAPESR
                     KSSHKDIQRFGKESKDFLTNLLRGKKVAFEYDVQKKDQYGRTLAYVYLEDGTFLNDYL
                     IRNGYAKVVTFPPNVKYHKRLLASERYARQHSLGMWKEK"
     gene            complement(492..1058)
                     /gene="efp"
                     /locus_tag="PKH68_03755"
     CDS             complement(492..1058)
                     /gene="efp"
                     /locus_tag="PKH68_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNX12084.1"
                     /translation="MATTADIKNGMCIDIEGQYYYITEFLHVKPGKGPAFVRTKMKNV
                     VTGRTLERTFNSGIRLEEVRIEHRPYQFLYKDDMGYNFMNNETFEQVSIAKEQINGVD
                     FLKEGMEIEVVTHADSDTILYADLPVQVALLVTYTEPGLKGDTATNATKPATVETGAT
                     VRVPLFINEGETIRVDTRDGSYVERVKA"
     assembly_gap    1373..1472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1473..1777
                     /locus_tag="PKH68_03760"
     CDS             <1473..1777
                     /locus_tag="PKH68_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12085.1"
                     /translation="ELAEFIWGSRKGEPWALTSAVDDLKIDFTLSPNPVRNQLTISSD
                     EPDLSYSIFNLNGQLLQENDLYGTQVISVDNLNNGMYLIQVKSGNRRTVQKFIVSH"
     gene            1857..3764
                     /locus_tag="PKH68_03765"
     CDS             1857..3764
                     /locus_tag="PKH68_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNX12086.1"
                     /translation="MKKLSFLLPLFFILSVSVFAQIPAGYYSAAVGKTGAALKTQLSS
                     IITAGAVDKGYDFLYTIYKTSDNLPNGKVWDMYSLKSDGTANYYYDHGGDKCGSYSNE
                     GSCYNREHTFCDSWLGNASPQRSDAHHLIPTDGYVNNRRSSFPHGKVGSASWTSSNGS
                     KLGSSDASTGYSGTVFEPIDEFKGDFARMYFYVATRYESKIGGWANNGSAGEILAGNS
                     YPAYKAWFYNLMLKWNAQDPVSQKEIDRNNAIYTNFQHNRNPFIDHPEFAEYIWGTKK
                     GQAWSESGSTSPTLFSPTSGSTVDFGTVIYQQTTTKTIVVSGMNLTGDLTLSISGADA
                     SYFSVPVTTIPKASAQAGYSLTISYNAPVVGTHNAILILSGGGITDATVNLTATATAT
                     NCQNLNFSAPFSSTMSPFTQYSVTGSQSWYWKSSAYGVAMSGYVNSTSNANEDWLISP
                     SLDLSTSKDVVLAFDHTINKGVVTNMPTENTVWISNNYSSGNPNTAIWTQLTIPTYPA
                     GNDWTFVNSGTISIPTDKCQLNTFIGFKYISTTAANSTWEIKNLTVNGTCISSAVYNA
                     NKSYKHKVFSTNKNISISNLENEDLVVYDICGKNVFSSRNLTGETSINSLNSGIYIIK
                     AGNEILKAMVK"
     gene            3787..4017
                     /locus_tag="PKH68_03770"
     CDS             3787..4017
                     /locus_tag="PKH68_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX12087.1"
                     /translation="MKKISQFKHIIKLNTLTTREKEILHLIAKGKTDKEIADKLFISI
                     RTAKKHRNNMITKIGVNNTVELVAFAVEEGEC"
     gene            4098..6119
                     /locus_tag="PKH68_03775"
     CDS             4098..6119
                     /locus_tag="PKH68_03775"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX12088.1"
                     /translation="MFAKSKHSKALLRLLSLLIFFALSQIMCAYTATSDTTQISHYLQ
                     LAQKHEAVSIDSVVYYFHKAVTLANSGLHSTTNQKVVKKLLMQLIKAELGLGHTYYEK
                     PDFEKSLQHNSRALESAKKIGDSFYIGESLFNMGEDMLEQCRYNEAMEKYGEAITYYR
                     KVNDETSVFWCYSGMGIVQKNAGNFDDAIECFKKALASAQKRSLKNEEASCYNNIGNV
                     YRRQGNFAKAMEAFQKALDQFIRLKKDLSVSDCLNNIGNLYLDINDPFRALNYYNRSL
                     RIIKDKNDDYRLIGRYKNLADAYTDLKDYDNAVHFLDDAIKLSEKSGNVSYLASCYTQ
                     YGKLQMSKGEFLISVAYFKKAESLFQKIDAKAEEAEALFEIASAEQQKGLVNDAILHA
                     NEVLKLAMISGALNIELKANKCLAKCYEQRGDAKKAIPYLNRAILLKDSIYSVEKYRT
                     IENIESGFKHNELKTENQILIQNSFLQKQAIRTKNLLAILLGISLILSAALFWMIVNR
                     YQNSKKEAIRLQNLKEQQIEKLNENLKMKERELTSKTIFINQKNQLLERLIRELEQLK
                     KADITVGAVSHLQTELKQELSPNAWKEFEIQFNEVHPDFQNQLLIRFPELSPSERRLC
                     SFLRLDMNTREIVSLTGQTFKSIEVARTRIRKKMSLSREDNLTNFISSI"
     gene            6267..7343
                     /locus_tag="PKH68_03780"
     CDS             6267..7343
                     /locus_tag="PKH68_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12089.1"
                     /translation="MKSIYYLLFLTCFLFVGSCSKDDYNDPDNNGSSDNKPHVYICGH
                     ESVGTNKIAKYWKDGTEVILTDGTHDANAYAIFVDGKDVYVAGGESDGTHRVAKYWKN
                     GVAVNLTDGKYDAIARDIFVSGKDVYVAGSVQDGKSSYYGGVYVAKYWKNGEAITLSD
                     GNNDASARAILVSNNMVYTAGTVFEPTYSNNIAVYWVNKQVKQLLTPNSPNQGSGALD
                     IAKTDNGNFIIAGSTHKGNTNLATYWKDEQQVNLTDGKVGTNLGSVYPSGADLYFAGW
                     RYKSEGDYTMLANYWKNGTETVLNDGTKDAEAYSICVSNSVVHVVGWENGNYKREAQY
                     WINGVAKRICKSQRWSEATDIVIK"
     gene            7384..8808
                     /locus_tag="PKH68_03785"
     CDS             7384..8808
                     /locus_tag="PKH68_03785"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNX12090.1"
                     /translation="MNKYRIILLFLFALLMGCSKNESDEVLESENTQKGSGSLVWLTS
                     AGGKLSQYGQKVVVDPEGYLYVIGYFNGTAAFGTITLVASGENNTFVAKVDTKGNFIW
                     AKGFGGTSLFTTDYPNSIAIDTDGNIYVTGMFDYKAEFGSITVNAHNGSTDIYIAKLD
                     KAGNFQWAKNAGGNQNVDHTYAICAGLNDNIYLTGIFYTKAWFDDIELSADKQGYYTF
                     LAAMDKAGNFKWVKRMGTTSLLSITDLTTDNNGDLFLLGYYFGDENLGGVALLNNTQY
                     AYTYVAKTNQYGVINWVQTTSSSDGGIFGRSVCADNSGNLYITGEFIKNGVVGSVSLS
                     SATKDAFWAKIDSQGNWKWAKQSQTGSVIAENFCAGKSISVDAEGNPFLSGNFMGNTV
                     LGNTTHQTNNFENGFWAKLNTDGSISWLKKTNSTGTANVNDLIPDNQGYLYLTGYYGN
                     KAAFDSIQPTWRGSTDIFVLKSKQ"
     gene            8882..11671
                     /locus_tag="PKH68_03790"
     CDS             8882..11671
                     /locus_tag="PKH68_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12091.1"
                     /translation="MKSLKNKIKVLVLLVVIHSMLAIKLMAADYTWTNLSAKLPANAP
                     ITDMAWLSNHFSHKIFLAAGSSLLQVNDDNTVTTISSNGNYAALYQKVDAGYLWVADG
                     SLLRTYSKSQGLSEIGIPLAGIASELWASDTEYKGYAVGSLGISLFDGIWGTVTAKTT
                     PGSGNISDIWAVDNAINSVYALSANGSVYRSTDGAASWGAVPGATDIRAMYFRNFNQG
                     WAAGTNGHIYKLNASGSWEQKNTGISDEFCSIAFTTSGSCGVAVAKNGTAVFSGDEGE
                     TWAQLQVSAGSSGITKAIITEDKKIYLAGNKALFVGIPALTTGHNNLPALVQMVSVRT
                     DGDSPFFGSYGSSVSNDGNYVVFASRDEKLVANDAYDSNNLFLWDGSKSSITMADTLA
                     NGNRADGISHFIQVSGDGKWLMLSSSGESLTSGANDHISLPIVDPITGSDVFLKNREN
                     GTISLASTFDEGGAYTGLDMSSDGSRVLYVDGTLSLYLYNRFNGSNAPIELGHYGSGT
                     GDGCHVLTGEGAISGDGSTIVYQTEDYMIVDDPGGGHAKSNMSRSVYVCTAGGASRLL
                     FKSSYADFAYDYQGVTYFSLNYDGRYFVHRDEKGQIKFWDLNTGTSEIVSKSTMGEQA
                     NGNCWATGISANGRYVVFYSAAGNLAPNDTNGKFDVFLRDRTSGKTTLVSTNIAGEPS
                     TERSNSEFDSSQNLSISADGQYITYESQADNLVSTPKNSSTRCIFWAMLDVEKDGLTI
                     SGELAVSTISPATNISATGAVVSGNVVAGSDVTVTERGFVYSASANPSVGGSGATKVT
                     AGNGAGTFSATLAGLASNTIYHVRAYASDGTEITYGEDVTFTTLMATGVIEKPTDGNF
                     MIFPNPTAGTVHISTPTGESINRVEVFSVQGKQVLSIDNVQENSINLSGLPKGIYLVR
                     IKSQSGFYNRKIVKN"
     gene            11684..12793
                     /locus_tag="PKH68_03795"
     CDS             11684..12793
                     /locus_tag="PKH68_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12092.1"
                     /translation="MRSITDMQIMLNLLLLILGGCSGGSSSETSGNEIQSGKQAILFH
                     RQNEPNENAFSFLLPQNWTVSGGITRVDPNSGGGAANAIEAKLYLKLSSPDNKASIAW
                     LPDTRFFDMRRYPGANMAASMFPDGSNYNGMTVLQMLSPGDFVKRVAFPFAHPHAQSL
                     QITGVQQLPELAEEYRRMSSAMVPGYQFNYVAAIVTLEYAENSIQYLEKMVCVIEDYG
                     RLGTGLWGNKETWYVRAEKGKFESMAPIFSTIGQSVKINPEWIRHEIRSQQINSQIAI
                     QTQRDVERIDKEITEHRARTNSEINNDMYLNLTGQEDYTNPFTGETERGTNEWNYRWE
                     NRQGDVIYSDNQNYSPNDDVNLTVKGFKRSEIRKR"
     gene            12840..15587
                     /locus_tag="PKH68_03800"
     CDS             12840..15587
                     /locus_tag="PKH68_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HNX12093.1"
                     /translation="MKTYLLFVTICISISFVHAQVPKTVTVNTPGTLESVLTSTELIN
                     TTSLVVKGKINELDFLTMRIKMSALVSVDISETDVMESTRNGYTYPANEIPKYALSEN
                     IKIKAIKLPSSANSIGVGAFRSCTGLRSFTNAVSVTTIQHEAFYNCKSLKTVEIPAKV
                     KTIQYEAFYQCDSLTLVNISASLVSFERNCFNFTASFTVDPKNLYFSALDGILYNKNK
                     TTLLQCPNSKSGNFSIASSVVTIGANAFRKCEKITGVIFSPVLDSIKDFSFIGCTGLT
                     ELNFPSSLRYIGNNAFFSCTNLKTVGFSDAMEFIGDDAFNECALIDSIVMPSKMKRIG
                     SCAFRYCTALKAVRFVAPSSLNEIGILTFQACTGLTSVSIPNSVKKLGGGAFQDCTNL
                     KEVSLPSSLPNISISLFKGCEKLTSVDIPSSVSKISDYAFLGCYSLSNLLLPTGVDSI
                     GYRAFGGCTKLKYANIPATVKFIGAEAYLNCKALSVLYVYTQTPVDLSSSDNVFGGFS
                     KSKCNLMVPSVSKNLYKTANQWKDFTLVGDIDVLLSEIKVNGIDVPTFHSMVFDYSMI
                     LPPETSIYVISANSLYPNATVKITQVASVPGAATIEVTGIDNTTKQIHTVYLAIADEH
                     SNIVTINSGVKTGDKILMIVDGTPGQAMVDYGDGILKTTDLFNYSGFTPYISFFDATS
                     PVDNPTIKVYAKSLKNLFVTALNKAISLDVSKNSGLEILICSDNQLTTLDVSKNTAII
                     QLECNNNKLRSIKCSAENTALKEIYCKGNQLSECGLDSLFYSLPFRSYENNGTLYVGD
                     NPGTLISKTEIALNKFWSTDISGDGSGCTHSGIDVSETVDLVLYPNPGNGRFNLCIPE
                     GQQLHSLTVVNSLGKTVRNVDISGQDSEFTFDLSDQPNGIYLLRCSTNKGLGCRKLVK
                     Y"
     gene            15774..16541
                     /locus_tag="PKH68_03805"
     CDS             15774..16541
                     /locus_tag="PKH68_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HNX12094.1"
                     /translation="MIDVTTGIPEDTIKYGVAFHECIDADGNSYKVVKIGDQTWMDEN
                     LKTTKFNDGTAIPYTKDNSVWSASVLASTPAYCWYSDDTYQNAYGALYNWYAVNTGKL
                     APVGWHVATDDEWTILGNYLMANGYNYDGTTTSNKYAKSLATTYSRFGSIFSAGCIGD
                     DLSKNNSAGFYALPGGYRDSGSGVYYLNQSAFWRSASENGTGGFGWNRSLLYYSAEMY
                     RDSYVIPTGMSICCVKDLFICLIVQRLLLLFSCNQEK"
     gene            16688..>16848
                     /locus_tag="PKH68_03810"
     CDS             16688..>16848
                     /locus_tag="PKH68_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNX12095.1"
                     /translation="MKIHEYQAKEIFARYAIPVTREILCYTTAEALAAAEKLGFPAVV
                     KAQVLTGGRG"
BASE COUNT         5152 a         3270 c         3625 g         4701 t
ORIGIN      
        1 cttcggtggc taactcctcc cgggaatacc gtaaggtcta taaagtcatt gacggcgata
       61 cattctggat gaaggatggg tttggacaaa tagagaaaat caggttgacg ggcatagatg
      121 cacccgaatc gcgcaaatca agccacaaag atattcaacg gttcgggaag gaatcaaagg
      181 acttcctgac caatttgctg cggggtaaaa aagttgcatt tgaatacgac gtacagaaaa
      241 aagatcagta tggccgtaca ttggcttatg tatacctcga ggatggcact tttctgaatg
      301 attatctcat caggaacggt tatgccaagg tagtcacatt tccacccaat gtaaaatacc
      361 acaaacgctt gcttgccagt gagcgatatg cccggcagca ttcgctggga atgtggaagg
      421 agaaataaca gacacaaaaa aatccggtag attttcatcg ccggattttt tatgatggaa
      481 aatgtgtgaa tttaggcttt tacccgctcc acgtatgagc catcgcgtgt gtctacccga
      541 atagtttcac cttcgttgat gaataacgga acacgaaccg tagcaccggt ttcaacggta
      601 gccggttttg tagcgttggt agctgtatcg cctttgagtc ccggctccgt ataagtaacc
      661 agcaaagcca cctgtacggg aagatcggcg taaagaattg tatcggagtc ggcatgtgta
      721 accacttcaa tttccatacc ttctttcagg aaatcaacac cgttaatttg ctctttggct
      781 attgaaactt gttcgaaagt ttcattgttc ataaaattat agcccatatc atccttgtac
      841 agaaactggt acggacgatg ctcgatgcga acttcttcga ggcgaatacc cgaattgaaa
      901 gtacgttcca atgtacgtcc ggtcactacg tttttcattt tggtgcggac aaaagccgga
      961 ccctttcctg gttttacgtg tagaaattca gtaatataat aatattgacc ttcaatatcg
     1021 atgcacattc cgtttttaat atctgctgtt gtagccataa aaaagtgttt ttttaaaata
     1081 ttattctgat aaattttttg gagtgcaaag ttagtgtttt tctttgaatt gcaaaaacaa
     1141 attgattatc agcttaatct gaagttgaat tttttatgat gagagatttg ttgccaatga
     1201 gcgtaatact tgaatttcat acaaaattga tgatataaat aaatgtctca aacttaataa
     1261 aatgatgcct gatttattac cattaaccat aatgtgagca ttccggatac gtttaacaat
     1321 gtttgtgata atttacatct aaatatttca atttttatgt aagatttaat acnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgaactg gcagaattta tctggggaag
     1501 ccgtaagggt gaaccctggg cgttaacatc agcggttgat gacttgaaaa ttgatttcac
     1561 cctgtcgccc aatccggtac gcaatcaact gacgataagt tcggatgagc cggatctcag
     1621 ttattccatt tttaatttga acggacagtt gttgcaggaa aacgatttgt atggcactca
     1681 ggttatctca gtggataatt tgaataacgg aatgtatctg atacaagtca aatccggtaa
     1741 ccgtagaacc gtacagaaat ttattgtcag tcactaaaat ccatagtcac ataaaatttg
     1801 cacaatccag cttggcaaat tctcaaaatt tattaatcca ataacatcat caattcgtga
     1861 aaaaactttc ctttttactc ccgttatttt ttattttatc agtatccgtt tttgctcaaa
     1921 ttcctgccgg atattacagt gccgcagtgg ggaaaacagg agccgcactt aaaactcaat
     1981 tgtcgtcaat tatcactgcc ggagcagtgg acaaaggata tgacttcctg tacacaatct
     2041 acaaaaccag cgacaacctg cctaacggaa aggtgtggga tatgtattcg ctcaaaagtg
     2101 acggaacggc aaattattat tatgatcatg ggggcgataa gtgtggaagt tacagcaacg
     2161 aaggcagctg ctacaaccgt gaacatactt tttgtgattc atggcttggt aatgcatctc
     2221 cacaacgttc agatgctcat cacctgattc ctaccgacgg ctatgtgaac aaccgccgca
     2281 gttcatttcc tcacggaaaa gtgggcagtg cctcatggac atcttccaat ggttcaaaac
     2341 ttggaagttc cgatgcatcc accggttatt caggaactgt tttcgaaccg attgacgaat
     2401 tcaagggtga ttttgcccgt atgtatttct atgtagccac ccgttacgaa tcaaaaattg
     2461 ggggatgggc taataacggt agtgccggtg aaattttggc aggaaacagt tacccggctt
     2521 ataaagcctg gttttataat cttatgttga aatggaatgc acaagaccct gtaagtcaaa
     2581 aagaaattga ccgaaacaat gcaatttata cgaattttca gcacaaccgt aaccctttta
     2641 ttgatcatcc ggaatttgca gaatatattt ggggaacaaa gaaaggtcag gcttggtctg
     2701 aatccggttc tacttcacct accttattct cacctacatc cggttctacg gttgattttg
     2761 gaacggttat ctatcaacaa accacaacta aaacaattgt agtatctggc atgaacctta
     2821 caggtgattt aaccttgagc ataagcggag cagatgcttc ctatttctcg gtacctgtta
     2881 caactattcc aaaagcaagt gcacaagctg gttacagtct tactatctcg tataatgctc
     2941 cggtagtagg aacgcacaat gctattttaa tcctttctgg tggaggaatt acagatgcaa
     3001 cagtcaatct gactgcgact gcaactgcta ccaattgtca gaatttgaat ttttctgcac
     3061 ctttctcctc tacgatgtct ccatttacac aatacagcgt aacaggaagt caatcatggt
     3121 attggaaaag ttcggcgtat ggagtagcta tgtctggtta tgttaattct acaagcaatg
     3181 caaatgaaga ttggttaatc tctccttcct tggatttatc taccagcaaa gatgtagtat
     3241 tagcatttga tcacaccatt aataaaggtg tagttacaaa tatgccaact gaaaatacag
     3301 tgtggatttc aaacaattat agtagtggaa atcctaatac agctatctgg acacaactta
     3361 ctattccaac ttatccggca ggaaacgact ggacatttgt aaattcgggc acgatctcca
     3421 ttccgactga caaatgccaa ttaaatacat ttatcggatt taaatacata tccactactg
     3481 cagctaactc aacttgggaa ataaaaaatt tgacagtaaa cggaacttgt atctcatctg
     3541 cagtttacaa tgcaaataaa agctacaagc acaaagtctt ttcaacaaat aaaaacatca
     3601 gtatctcaaa tcttgaaaac gaagatcttg tcgtatatga tatttgcgga aaaaatgtat
     3661 tctcaagcag aaatttgaca ggagaaactt caataaattc acttaattca ggtatataca
     3721 ttatcaaagc cggaaatgag attttgaaag caatggtgaa ataatcataa tcaattaaat
     3781 tcggcgttga agaaaataag tcagttcaaa catatcataa aacttaatac gctaacaaca
     3841 cgcgaaaagg agatactaca tttaattgca aaagggaaaa cagataaaga aattgccgac
     3901 aaattattta tcagtattcg tactgctaaa aaacacagaa ataatatgat tacgaaaatt
     3961 ggagtaaata atacggtcga attagtggct tttgctgtag aagaaggaga atgctgaaca
     4021 atggacatta aaaattgtcc gttttaaaat agtaaccaaa caaaagttct tttttattac
     4081 attcattgca aatacaaatg ttcgccaaat caaaacacag taaagcattg ttacgcttat
     4141 tatcactgct gattttcttc gcattatctc aaattatgtg cgcatatact gctacatccg
     4201 acacaactca aatttcgcat tatttgcaat tggctcaaaa acacgaagct gtttctatcg
     4261 attcagtcgt ttattatttc cataaagcgg ttactttggc taattccgga cttcattcaa
     4321 caaccaatca gaaggttgta aagaaattac ttatgcaatt gatcaaggca gaacttggac
     4381 ttggtcatac ctattatgaa aagccggatt ttgaaaaatc gcttcaacac aattcacggg
     4441 cactcgaaag tgctaagaag attggcgact ccttttatat tggcgaaagc cttttcaata
     4501 tgggagaaga tatgcttgag caatgtcgtt acaacgaggc tatggaaaaa tacggcgaag
     4561 cgattaccta ttaccggaaa gtcaatgatg aaacaagtgt gttttggtgc tattcgggaa
     4621 tgggaattgt tcagaaaaat gccggaaatt ttgatgatgc cattgaatgt ttcaaaaagg
     4681 cattagcttc ggctcaaaaa agatcactga aaaatgaaga agcttcgtgt tacaacaata
     4741 ttgggaatgt ttaccgcagg cagggtaatt ttgcgaaagc catggaagcc tttcaaaaag
     4801 cccttgatca atttattaga cttaaaaaag atttgtcggt ttcagactgt ttgaacaaca
     4861 ttggaaatct ataccttgat attaatgatc cttttcgggc gttaaattac tacaataggt
     4921 ctcttcggat tatcaaagat aaaaacgacg attaccgact gataggacgg tataaaaatt
     4981 tggctgacgc ttacaccgat ttgaaggatt atgacaatgc ggtgcatttt ttggatgatg
     5041 ccattaaact gtctgaaaaa tcgggaaacg tatcgtatct tgcttcatgt tacacccaat
     5101 acgggaagct gcaaatgtca aaaggtgagt ttttgatctc cgttgcatat tttaaaaaag
     5161 cggaatcgct ttttcagaaa attgatgcca aagccgaaga agcagaagct ttatttgaaa
     5221 tagccagtgc ggagcaacag aaagggttgg tcaatgatgc catacttcac gcgaatgaag
     5281 tcttgaaatt agcaatgatt tcgggagcat taaatatcga gttgaaagca aataaatgct
     5341 tagccaaatg ttatgaacag aggggggatg caaaaaaggc cattccgtat ctaaatcgag
     5401 ccatactact gaaggatagt atctatagtg tagaaaagta ccgaacgatc gaaaatatag
     5461 aatctggatt caaacacaat gagcttaaga ccgaaaacca gattttaatc caaaacagtt
     5521 ttttgcaaaa gcaagctatc cgaacaaaaa atttgctggc tattctgctt ggaatcagtt
     5581 taatattaag tgccgcctta ttttggatga tagtcaatcg gtatcaaaat tcaaagaagg
     5641 aagctattcg gctgcagaac ttgaaggaac agcaaattga aaaactgaac gagaatctta
     5701 aaatgaaaga gcgcgaactc acgtccaaaa ctatttttat caatcaaaaa aatcagttgc
     5761 tcgaaagact aatccgcgaa ttggagcaac taaaaaaagc tgacatcaca gtaggagctg
     5821 ttagtcatct tcaaacagag ctaaaacagg aattatctcc caacgcctgg aaagagtttg
     5881 agattcagtt taacgaagtt catcccgatt ttcaaaacca acttttaatc cgcttccccg
     5941 aactatctcc ttcggaaaga aggctttgtt cctttctcag gttggatatg aatacccgcg
     6001 aaattgtctc gcttacaggt caaaccttta aaagcataga ggttgcccgc actcgtatcc
     6061 gcaaaaaaat gagcctgtcg cgggaagata accttaccaa ttttatatct tccatttaaa
     6121 ttcccatccc aattccccaa tttaattcgt acaataatac aatgagtgtt tttgtagggt
     6181 ggctcctttg tatttgtagg gaacattatt aaactgtagt cgtacaatta cagtaacttt
     6241 gtgtttgaaa tcaaaaatca acgatcatga aatcaatcta ttatttgctt tttttaacct
     6301 gctttttatt tgtcggttcg tgcagcaaag atgactacaa tgaccccgac aataacggtt
     6361 cctctgataa taagccccat gtttatattt gtggacatga atctgtcgga accaacaaga
     6421 ttgccaaata ttggaaagat ggcactgaag ttatcctgac cgatggcaca cacgatgcca
     6481 atgcctatgc aatttttgtc gatgggaaag atgtgtacgt tgccgggggc gaatcggatg
     6541 gaacgcaccg ggtagctaaa tactggaaaa acggcgttgc ggtaaacctg accgatggca
     6601 aatacgatgc gattgcaagg gacatttttg tttcgggcaa agatgtgtat gtggcaggct
     6661 cggtgcagga tggtaaatct tcctattacg ggggagttta tgtggccaaa tactggaaaa
     6721 atggcgaagc aataacactc tctgatggaa ataatgatgc ctcggcaagg gcgatcctgg
     6781 tttcgaacaa catggtttat accgcaggaa cggtgtttga gccaacatac tccaacaaca
     6841 tagctgttta ctgggtaaat aaacaggtca aacaactgct aacgcccaat agcccaaatc
     6901 aggggtcagg ggctttagac attgcaaaaa ccgataatgg aaattttatt atcgcaggta
     6961 gcacccataa aggaaatacc aatttggcta cttactggaa agacgagcaa caggttaacc
     7021 tgaccgacgg gaaagtaggc acaaacctcg gatcggttta cccttcagga gcagacctgt
     7081 attttgcagg ctggcgatac aaaagcgaag gcgattacac catgctcgcc aattactgga
     7141 aaaatggaac tgaaactgtt ttgaacgatg ggaccaaaga tgcggaagcc tattcaatat
     7201 gtgtatccaa ctcggtagtg catgtggttg gctgggaaaa cggcaactac aaacgggagg
     7261 cgcagtattg gataaatggc gttgcaaaaa ggatatgcaa aagccaacga tggtcggaag
     7321 ctacggatat cgtgataaaa taagctgaac attaaactat tggcaactaa taaattatcg
     7381 gatatgaaca agtatcgtat tatcttgctt tttctatttg ccctccttat gggttgttcc
     7441 aaaaacgaat cggacgaagt actggaatct gaaaatacac agaagggaag cggctcgctg
     7501 gtctggctta cctctgccgg cggaaagctg tcacaatacg ggcagaaagt ggtcgttgat
     7561 ccagaaggct atttgtacgt gatcggctat tttaatggca ctgctgcttt tggaacgatt
     7621 acattggtag cttcgggtga gaataacacc tttgtggcaa aagtagatac caaaggtaat
     7681 tttatctggg ctaaaggctt tggtggaaca tcactgttta ctactgatta tccgaattca
     7741 attgccattg acacggatgg caatatttat gtaaccggca tgttcgacta caaagccgaa
     7801 ttcggttcga ttacggttaa cgcacacaac ggctccaccg atatttatat cgctaaactc
     7861 gacaaagccg gcaattttca gtgggctaaa aatgcgggtg gcaatcagaa tgtggatcat
     7921 acttatgcaa tctgtgccgg actgaacgac aacatatacc ttaccggaat tttttatacc
     7981 aaggcatggt tcgatgacat tgaactatcg gctgacaagc aaggttacta tacttttctg
     8041 gccgccatgg ataaagccgg caactttaag tgggttaaac gaatggggac aacttccttg
     8101 ttgagtatca ccgatctgac aaccgataac aatggggatc tatttctgct cggatactat
     8161 tttggcgatg aaaacctggg aggagtagcg cttctgaaca atactcaata tgcttatact
     8221 tatgtggcaa aaaccaacca atacggtgta atcaactggg ttcaaacgac cagtagcagt
     8281 gacgggggga ttttcgggag gtcagtttgt gccgataatt ccggcaattt gtacatcaca
     8341 ggagaattta tcaagaatgg cgttgtgggg tccgtttcgc tgtcgtcggc aaccaaagat
     8401 gctttttggg caaaaatcga tagccaggga aattggaaat gggccaaaca gtcccaaacc
     8461 ggaagtgtga ttgctgaaaa tttttgtgca ggaaaatcca tctccgtcga tgcggaagga
     8521 aacccattcc tatcggggaa ttttatgggg aataccgttt tggggaacac aacacatcaa
     8581 accaataatt tcgaaaacgg gttctgggcc aaattaaata ccgatgggag tattagctgg
     8641 ttgaaaaaaa ccaattcaac cggaacggcc aacgtaaacg atttgatccc cgacaatcag
     8701 ggatatctct acctgactgg gtattacggc aataaagcgg cttttgattc catccaacca
     8761 acctggaggg gaagcaccga tatctttgtc ctgaaatcga aacaataggt agttgatgct
     8821 gataatcagg agataagatt taattcaggg taaagaaaat aggcaaaaaa tattttaagt
     8881 tatgaaatca ctaaaaaata aaatcaaggt acttgtattg ttggtagtta tacacagtat
     8941 gttggctata aaactaatgg cggccgatta tacctggacc aacttgtcgg caaaattgcc
     9001 tgccaatgct cccatcaccg acatggcctg gttaagcaac catttcagcc acaaaatttt
     9061 ccttgccgcc ggaagctcat tgttgcaggt caacgacgac aacacagtaa caacaatcag
     9121 cagcaatgga aattatgctg ctttatatca aaaggttgat gccggttacc tttgggtagc
     9181 cgatggctct ttgttgcgca cgtacagtaa atcccaaggt ttgtcggaaa tcgggattcc
     9241 gttggctgga attgccagtg aactgtgggc atcggatacc gagtataaag gatatgccgt
     9301 gggtagcctg ggaatcagtt tatttgatgg catctggggg actgtaactg ccaaaaccac
     9361 acccgggtcg ggcaatattt cggatatatg ggcagttgac aatgcgatta actcagtata
     9421 tgcactttct gcaaatggtt cagtttaccg gtcaaccgat ggcgctgctt catggggcgc
     9481 cgttcccggg gcaacggata tccgggccat gtatttcagg aattttaacc aaggatgggc
     9541 tgccggaacc aacgggcata tttataaatt aaacgcttcg ggaagctggg aacaaaaaaa
     9601 tacgggcatc agcgacgaat tttgctcaat agcgtttact acttccggat cgtgcggtgt
     9661 tgctgttgcg aaaaatggca cggccgtatt ttcaggcgac gagggtgaaa cgtgggcaca
     9721 gcttcaggtc tctgctggtt cgtccgggat taccaaagca attattaccg aggataaaaa
     9781 gatatacctg gccgggaata aagctctttt cgttggcatt ccggctttaa ccacaggcca
     9841 taacaacttg cctgccctgg tgcaaatggt ttcggtacga actgatggcg actcgccttt
     9901 ttttggaagc tatggcagtt cggtttccaa tgatggcaat tatgttgtct ttgcttccag
     9961 ggacgaaaaa ttagtggcaa acgatgccta cgactccaat aatcttttct tatgggatgg
    10021 ttccaaaagt tcaataacga tggccgacac tttggcaaac gggaacaggg ccgatggaat
    10081 ttcacatttt atccaggtca gcggcgacgg gaaatggctg atgttgtcga gcagcggcga
    10141 atcattgacc agcggggcaa acgatcatat ttccttgcct attgttgatc ccattactgg
    10201 ctcggatgtg tttttgaaaa acagggaaaa tgggactata agcctggcca gtacatttga
    10261 tgagggggga gcatatacgg gactggatat gagttcggat ggaagccggg ttctttatgt
    10321 ggacgggact ctttctctgt atttatacaa tcggttcaac ggatcaaatg caccaattga
    10381 gcttggccat tatggctcag gtactgggga tggttgccac gtgcttaccg gagaaggcgc
    10441 aataagcggg gatggaagta ctattgttta ccaaactgaa gattacatga ttgttgacga
    10501 ccccggaggc ggccatgcaa agagcaacat gtccaggtcg gtttatgttt gcacggctgg
    10561 cggcgcttca cgtttgcttt ttaaatcgag ctatgccgat tttgcctatg attatcaggg
    10621 agtgacttat ttcagcttaa attacgatgg tcgttatttt gttcatcgcg acgagaaagg
    10681 ccagataaag ttttgggacc tcaataccgg cacgtccgaa atcgttagca aatcaacaat
    10741 gggcgagcag gcaaatggta attgttgggc aactggtata agcgccaatg ggcggtatgt
    10801 ggttttttat tcggcagccg gcaacctggc cccaaacgat acaaacggga agtttgatgt
    10861 ctttttgcgc gaccggacat caggcaaaac aactttggtc agtacaaata tcgccggaga
    10921 gcccagtacc gaacggtcga attcggagtt cgatagttcc caaaacttaa gcattagtgc
    10981 cgacgggcag tacattacgt acgaatctca ggccgacaat ttggtttcaa cgcctaaaaa
    11041 cagttccacc cggtgcattt tctgggccat gctcgatgtt gaaaaagatg ggcttacaat
    11101 atccggtgaa ttggccgtca gtactatatc cccggcaacc aacatttcgg ctacaggcgc
    11161 tgttgtatcg ggcaatgtgg ttgcaggcag cgatgtaaca gtgaccgagc gcggatttgt
    11221 gtacagcgca tcggccaatc cttcggttgg tggttctggt gcaacaaaag taactgccgg
    11281 caatggcgcc gggacttttt cggcaacact ggccggattg gcttcaaata ccatttatca
    11341 tgtacgggcg tacgcttccg atggcaccga aataacatac ggcgaagacg tcacatttac
    11401 aaccttgatg gcaactggcg ttattgaaaa accaacagat gggaatttca tgatttttcc
    11461 aaacccaact gcgggaactg tccacatttc aacgcctaca ggcgaatcga taaaccgggt
    11521 cgaagttttc agtgttcaag gtaagcaggt tttgtcaatt gacaacgttc aggagaacag
    11581 cattaatttg tctggcctac ctaaaggcat ttaccttgtc aggataaaaa gccaatccgg
    11641 tttttataac cggaagatcg taaaaaatta gaaaactgtt aaaatgagat caattaccga
    11701 tatgcaaatc atgctcaact tattattact gattttggga ggctgctccg ggggaagtag
    11761 ttctgaaaca tctggaaatg aaattcaatc agggaaacag gcgattttat ttcaccgtca
    11821 aaacgaaccc aacgaaaatg cgttctcgtt cctgctgcca caaaactgga cggtttcagg
    11881 tgggattacc cgggtcgacc cgaacagcgg cggtggtgcc gcgaatgcca tcgaagccaa
    11941 attatacctg aaactatcgt cacccgataa caaagcttcg attgcatggt tacccgatac
    12001 ccgctttttc gacatgcgcc gttatccggg tgcaaacatg gctgcttcga tgttccctga
    12061 tggcagtaat tacaatggga tgactgtttt gcaaatgctg agtcccggcg attttgtaaa
    12121 acgggttgct ttcccgtttg cgcacccaca tgcccaatcg cttcaaataa ccggagttca
    12181 gcagcttccg gaactggcgg aggaatatcg ccgaatgtcg tccgcgatgg ttcccggtta
    12241 tcaatttaac tatgttgcgg ccattgttac gctcgaatat gccgagaaca gcattcagta
    12301 cctcgaaaaa atggtctgcg tgatcgaaga ttacggacgg ttgggcaccg gtttgtgggg
    12361 gaacaaagaa acctggtatg tgagggcaga aaaagggaaa tttgaaagca tggctccgat
    12421 tttttcaacc atcgggcaat cggtaaaaat caatcctgaa tggattaggc atgaaatacg
    12481 cagtcagcag ataaacagcc agatagcgat acaaacgcag cgcgacgttg agcgcatcga
    12541 caaggaaatc accgaacacc gggcgcggac aaattccgaa atcaacaacg acatgtacct
    12601 gaaccttacc gggcaggaag attacaccaa cccatttacc ggcgaaaccg agcggggaac
    12661 caacgagtgg aattaccgtt gggaaaaccg gcagggcgat gtgatttatt ccgataacca
    12721 aaattatagt ccaaacgacg atgtgaactt aacagtcaaa gggtttaagc gttcggaaat
    12781 caggaaacga taaacattta ctccgtaatg ccaaaacaga aaaccataaa taatcaacaa
    12841 tgaaaaccta tttactcttt gtaacgattt gtatttcaat tagttttgtt catgctcagg
    12901 taccaaaaac ggtaacagtg aatacgcccg gaaccttgga atctgtactt acctctactg
    12961 aattgattaa cactacttcc ttggtggtaa aggggaaaat caacgagttg gattttttga
    13021 ccatgaggat aaaaatgtct gcattggttt cggtagatat tagcgaaaca gatgtgatgg
    13081 aatcaacgcg aaacggatat acttatccgg cgaatgagat accaaaatat gcattatcag
    13141 aaaatattaa aatcaaggca attaagcttc catcttcagc taattccatc ggagtgggag
    13201 cttttcgaag ctgtaccgga ttacgctcat ttaccaatgc tgtttctgtt acaaccatac
    13261 agcacgaagc tttttacaat tgtaaaagcc ttaaaacggt tgaaattcct gcaaaggtaa
    13321 agactattca atacgaagcc ttttaccaat gcgattctct gactttagtg aacatttcag
    13381 cttccttagt gagttttgaa cgtaattgtt ttaattttac ggccagtttt acagttgatc
    13441 cgaaaaacct ttacttttct gctctggatg gtattttgta taataaaaat aagacgaccc
    13501 ttcttcaatg ccctaattct aaaagcggca atttcagtat tgcatcttca gtggtaacta
    13561 ttggtgccaa tgctttcagg aaatgtgaaa aaatcactgg tgttattttt tctccggtgt
    13621 tggatagtat taaagatttc tcttttattg gatgcacagg gctaactgaa ctaaattttc
    13681 cttcttcgtt aagatacatt ggaaacaatg ccttcttttc gtgtacgaat cttaaaacgg
    13741 ttggattttc agatgccatg gagtttattg gagatgatgc ttttaatgaa tgtgccttga
    13801 ttgattcaat tgtcatgcct tctaaaatga agagaattgg tagctgtgct ttccgttatt
    13861 gcacagcact gaaagctgtg cggtttgttg caccatcttc gctaaacgaa attggcattt
    13921 tgacatttca agcttgtacc ggattaactt cggttagcat tccaaattca gtaaaaaaat
    13981 tgggtggagg ggcttttcag gattgtacca atttgaaaga agtatccctt ccatcatccc
    14041 ttccaaatat ttcaatttcg ctttttaaag gatgtgaaaa gcttacatca gttgatattc
    14101 caagttcggt atcaaaaatc agtgattacg catttttggg atgttattct ttatctaatt
    14161 tgcttttacc taccggggtt gactccattg gatatcgggc atttggtggt tgtaccaagt
    14221 taaaatatgc taatattccg gcaactgtaa aatttattgg agcagaagcc tatttaaact
    14281 gcaaagccct ttcggttttg tacgtttaca ctcaaacccc ggtagattta agttcgtctg
    14341 acaatgtatt tggaggattt tcaaaaagta aatgtaattt gatggttcct tctgtttcaa
    14401 aaaatctgta caaaacagcg aatcaatgga aagactttac tttggtcggg gatatagatg
    14461 ttctgttgtc tgaaattaaa gttaacggaa ttgatgttcc aacttttcac agtatggttt
    14521 tcgattacag catgattttg ccacccgaga catccattta tgtaatatca gccaacagtc
    14581 tttatccaaa tgcaacagtt aaaataaccc aggtggcttc tgttccaggc gctgcaacca
    14641 ttgaagttac cggtatagat aatacaacaa aacaaatcca cacggtttat ctggctattg
    14701 ccgatgagca ttcgaatatt gtaacaatta attcgggagt aaaaacaggg gataaaatct
    14761 tgatgattgt tgatggaact ccgggacagg cgatggttga ttatggcgat ggtattctga
    14821 aaacaacaga cctcttcaat tattctggtt tcacccctta tatatccttt tttgatgcca
    14881 cgtcgccggt cgataacccc accattaaag tttatgccaa atcattaaaa aatttatttg
    14941 tgactgcact taacaaagcg attagtttag atgtgagtaa aaattcaggt ttggaaatac
    15001 tgatatgctc tgacaatcaa ttgacaacat tggatgtcag taagaatact gctataattc
    15061 aactggaatg caataacaac aaacttagat cgataaaatg ttctgcggaa aataccgctt
    15121 tgaaagaaat ttattgcaaa gggaaccaat tatctgaatg tggtttggat tctttgttct
    15181 attccttgcc ttttcgaagc tacgaaaaca atggaacgct atatgttggc gataatccgg
    15241 ggacattaat cagtaaaaca gaaatagctc taaataaatt ttggtcgacc gatatctcag
    15301 gcgatggttc aggatgtact cattcaggta ttgatgtatc ggaaactgtt gatttggttc
    15361 tgtatccaaa tcctggaaat gggagattca atctgtgcat acctgagggt caacaattgc
    15421 attccttgac ggttgtcaat tctttgggga aaaccgtccg aaatgttgat atctcgggtc
    15481 aggattcaga atttactttc gatcttagcg accaaccaaa cggcatttac cttttgcgct
    15541 gttcgacaaa taagggactg ggttgcagga agcttgttaa atactaaaac aaattcgaca
    15601 tgaaaacaat cacatttatc gcatagtttt ttttgttgga tttcatcttt gtgctcagga
    15661 tataaactta tcgttttaaa tccaaatctg gcgatggctc aatagatagc atcagggcaa
    15721 caatccttaa aacaaaagaa tcagttttga tatcgggaaa aagtaccctg aaattgatcg
    15781 acgtaaccac aggtattcca gaagatacca taaaatatgg cgtggctttt catgaatgta
    15841 ttgatgctga tggtaatagt tataaggttg taaaaatcgg tgatcagacc tggatggacg
    15901 aaaacctgaa gacaaccaag ttcaacgatg gcaccgctat tccctataca aaagataatt
    15961 ctgtatggtc agcatcagtt cttgcatcaa ctcctgcata ttgttggtat agcgatgata
    16021 cctatcaaaa tgcatacggg gctttgtata attggtatgc cgtaaacaca ggcaagttag
    16081 ctcctgtcgg ctggcacgta gctaccgatg atgagtggac aattctcgga aattatctga
    16141 tggctaatgg ttataattac gatggtacaa ctaccagcaa taaatatgca aaatcattag
    16201 caactaccta ttctcggttt ggctctattt tttcagcagg ttgcattggt gatgatttat
    16261 caaagaacaa ctcagccggg ttctatgctc ttccgggtgg ctatcgtgac agcggtagtg
    16321 gagtttacta tcttaatcaa tccgcatttt ggaggtctgc ttcggagaac ggtacggggg
    16381 ggttcggctg gaacagatcc ttactctatt actccgctga aatgtacaga gacagctatg
    16441 tcataccaac tggtatgtct atctgttgtg tgaaggattt gtttatttgc ttaatagtgc
    16501 aaaggttgct tttattattc agttgtaatc aagagaaata agaggctgtc tgtcttttga
    16561 gacagtctct tatttttatc taaaatttaa aaaaaataat caaaacaaaa taattaccta
    16621 tctttagaac aaaattaaat aaaataatgt tggatgtaaa aaatatataa aaatttctca
    16681 aaaaaacatg aaaatacacg aatatcaagc gaaagaaatt tttgcccgtt atgccattcc
    16741 ggttacacgt gaaattcttt gctacactac agcagaagct cttgccgctg ctgaaaaact
    16801 cggatttcca gccgtagtaa aagctcaggt gctcacaggt ggacgcgg
//
LOCUS       DAOPZK010000038        16695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3664_length_16695_cov_20.635817, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000038 DAOPZK010000000
VERSION     DAOPZK010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16695
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3664_length_16695_cov_20.635817"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..3706
                     /gene="purL"
                     /locus_tag="PKH68_03815"
                     /gene_synonym="purI"
     CDS             26..3706
                     /gene="purL"
                     /locus_tag="PKH68_03815"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNX12096.1"
                     /translation="MILFFRTPDQLIYAVQSPQPLDFDINKLIWLFGDAELLKEDKIS
                     GKFIGPRREMITPWSTNAVEITQNMGIKGILRIEEFSPVLEKDDHDPMLQRIYTELNQ
                     DIFTINKLPEPIQNIDDIAAYNAKEGLALSDDEIEYLNSISEKMGRKLTDSEVFGFSQ
                     VNSEHCRHKIFNGQFVIDGEEKELSLFKLIKKTSLDNPNNLVSAYKDNVAFNRGPVIE
                     QFSPKSDEKPDFFETKNIESVISLKAETHNFPTTVEPFNGAATGTGGEIRDRLGGGKA
                     SLPIAGTAVYMTSYPRNASKPWEKTLKERKWLYQTPEQILIKASNGASDFGNKFGQPL
                     ICGSLLTFEHQENDKTYGYDKVIMLAGGVGYASRRDALKGEPAPGEKVVVMGGDNYRI
                     GMGGGAVSSVETGQYANAIELNAVQRANPEMQKRVANVIRALAESDHNPIVSIHDHGA
                     GGHLNCLSELVEATGGVIEMDKLPVGDPTLSAKEIIGNESQERMGLLLPENEIERIQQ
                     IAERERAPFYVVGETTGDMQLTFKAPTQPDPIHLPLEYMIGNPPRTVITDTSVEENFA
                     SPEINEEQLLEYIQHVLQMESVACKDWLTNKVDRSVTGKVARQQCVGEIQLPLSDVGV
                     VALDYRGKTGIATSIGHAPLAALADPEAGSVLAIAEALTNIVWAPLKDGLDSVSLSAN
                     WMWPCKNPGEDARLYKAVEACSDFASALGINIPTGKDSLSMTQKYDAEKVFSPGTVII
                     SAGAEVSDIRRVVSPVLVNNPATSLLYVDFSFDKHKLGGSVLAQTMNKIGNEVPTITD
                     PEYFKAAFDTIQELISKKLIIAGHDISEGGMITALLEMCFANAKGGLKINLDHLAEPS
                     MINILFAENPGVIIQVENKAEVEKILRNNGIGYAAIAKPIAERRMEIHRKKTSYSFSI
                     NELRDQWFEASYLLDRRQSGELLAKARFENYKNQQLQFLFPAEFTGKLSQFALSPDRK
                     PGGVKAAIIREKGTNGEREMAYALYLAGFDVKDVHMTDLASGRETLEDINMIVFCGGF
                     SNSDVLGSAKGWAGGFLFNEKAKAALDNFYKRPDTLSLGICNGCQLMVELNLINPEHT
                     NRAKMLHNDSHKFESGFVSLSIPENNSIMLGSLSGSKIGVWVAHGEGKFSLPMGENAY
                     NVIAKYNYEAYPGNPNGSDFSVAGICSADGRHLAMMPHPERAVFPWQCANYPAERLHT
                     DEITPWMEAFVNARIWVEERTK"
     gene            3806..4717
                     /locus_tag="PKH68_03820"
     CDS             3806..4717
                     /locus_tag="PKH68_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX12097.1"
                     /translation="MNQTSLKKFAYLSIAAAVMTIGLKFGAYLLTGSMGLFSDALESC
                     VNLIAAVIALIMITISERPADKEHEYGHSKAEYFSSAIEGSLILVAAFSIIWSAVPRI
                     INPQPLENVGIGLLISTGASAINLAVSLILIKNGKKRHSLLLEADGKHLMTDVWTSVG
                     VIAGIGLVKLTGLIILDPIIAVIVALNIVWTGAQLLRRSGNGLLDSVISTDEQKLITD
                     FLETQKSKGIEYHSLMTRQAGQRIFISLHLLVPGAWTVQQGHDESDYIEEYIENLFEG
                     PVTCSTHIEPVEDPASLNDIGIDRKRK"
     gene            4822..5475
                     /locus_tag="PKH68_03825"
     CDS             4822..5475
                     /locus_tag="PKH68_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12098.1"
                     /translation="MKTVTLFLMAVVLNSISLHAASEPHFLKTTYSNGEFITVCQTEV
                     NAGETQTNAVIARMIDEFHTKPVNLFDWALKNLGMQGQKNNEMIVVLKTSKFDKKTGI
                     THGLVDVEIPYLQDFRNIKIDAVVKKENQPNGQFKATADVLQSGLLLKKAYGTVTAIP
                     ANEDRQILQTVTRIKFGWFLNLFISQKRYQTIVEWRVREFTKNLKLEAEKTIIATPN"
     gene            5525..6613
                     /gene="prfA"
                     /locus_tag="PKH68_03830"
     CDS             5525..6613
                     /gene="prfA"
                     /locus_tag="PKH68_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNX12099.1"
                     /translation="MAENSLLEKLEGLQHKFEEISMLITDPAVIADMKRYIKLNKEYN
                     ELNKIMTAVKEYKTALTNISEAKEIYDKESDAELREMAKAEIDDLEPQIPVMEENIKL
                     LLIPADPEDNKNVVMEIRGGTGGDEAAIFAGDLFKMYTRYCENRGWKVEVTDFSEGTI
                     GGYKEIIFTVSGENVYGILKYESGVHRVQRVPVTETQGRVHTSASTVAVLPEADEFEV
                     DINPADIDFHTSRSGGAGGQNVNKVETKVQLTHRPTGIVVVCQQARTQLGNREIAMEM
                     LRTKIYNMEYQKHIDAIASKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTVYNLVA
                     FMNGDIQDVIDKLIIAENAERMKESDLA"
     gene            6614..7138
                     /locus_tag="PKH68_03835"
     CDS             6614..7138
                     /locus_tag="PKH68_03835"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HNX12100.1"
                     /translation="METVIKYSKLKPVLLLLVSTAFAGICIWMLIESEGKNLLGYAGF
                     AFFFLLSVYIVYRLVKGGGIILSDKGIVTDRNHPEDSFINWDEIEKFTLREADGIKTI
                     NIHLYNPQVFLERQTRKNVRKKMEINEILYKAPVGITPNLMNISYKRLENLLIEKLES
                     NQSSKKRLTPNNHD"
     gene            7131..7961
                     /gene="pyrF"
                     /locus_tag="PKH68_03840"
     CDS             7131..7961
                     /gene="pyrF"
                     /locus_tag="PKH68_03840"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNX12101.1"
                     /translation="MTKQELFENIKRKESFLCVGLDTDINKIPEHILNISDDPIFDFN
                     VEIIKATADLCVAYKPNLAFYESIGLAGWDALERTVNFIRAEYPDQFIIADAKRGDIG
                     NTSAMYARTFFGNMDVDAVTVAPYMGEDSVLPFLTYENRWVILLALTSNKGAFDFQYM
                     EENGEKLFEKVLKTSQQWASAEKMMYVVGATKAEMLSDIRKIIPNHFLLVPGVGAQGG
                     SLSEVAQYGLNDMCGLLVNSSRQILYASTEADFKDAARNEAKRLQIEMAEILRSNHLV
                     "
     gene            8036..>8616
                     /locus_tag="PKH68_03845"
     CDS             8036..>8616
                     /locus_tag="PKH68_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12102.1"
                     /translation="MSEFSTHQRQRLADLRILFDAITYKKNAAEAIRQYQKTIENTIP
                     DDVVILVHQLMESNYPMEDIKSGINKMLNVLRRSIDAYPYTSPTDDTFFAVCIRNNNE
                     VKNLLNSLRPYILDINKKGISDEITSGMLRLWKEVQKFTQYYIIKENVLFPLVEKYLP
                     EYRCIAVMWSIHDDIRRNLSDLINLLSTDDLELP"
     assembly_gap    8617..8716
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8717..9387
                     /locus_tag="PKH68_03850"
     CDS             <8717..9387
                     /locus_tag="PKH68_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNX12103.1"
                     /translation="RNLSDLINLLSTDDLELPKLNRLTGEVFYNIYSIAFREERLLYP
                     MISKNVPAAEIEKMWNESVEIGFPYINPDMKTQHPEVKNDPSDLIDLKSGLLSAEQIF
                     LIFSHLPVDITYVDENNKVRFFSTPKKRIFTRTNAVLGRDVRNCHPHESVHVVEQIVE
                     SFRNGTKDNADFWINMRGKKILIQYFAVRNAENEYKGVIEVTQEISEIQALEGERRIL
                     DWNN"
     gene            9475..9762
                     /locus_tag="PKH68_03855"
     CDS             9475..9762
                     /locus_tag="PKH68_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12104.1"
                     /translation="MNDFVDYVEYTRPDNAKVRVAFEMLGSEVLFYFCEYQHSPTAEW
                     KLIQSPNNENFNYTAPDFQGEMRHYNIQIPTKEEIEKAQQLIVNKYNILSI"
     gene            9996..10646
                     /locus_tag="PKH68_03860"
     CDS             9996..10646
                     /locus_tag="PKH68_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12105.1"
                     /translation="MKILSNLFCTLSVLFFFSSCDEILKPGTTLGGSQSPMGEVGVIV
                     TSSSAPIAGVSGFSASVTSLTDGVSTYSAQATVTNALLKNMVSDFPGVTVNGNTVTIT
                     GLKIQQTTEGIKCLTGDGAGIIVKYSSAVGDTYPVGDTGNKRKVVSKSTDDDYPYGFY
                     NIKTIQVETDANNLGSTGGIDKITYIANHKFGLVGVKITFNDATSVTFPVYTNAQN"
     gene            complement(10731..10804)
                     /locus_tag="PKH68_03865"
     tRNA            complement(10731..10804)
                     /locus_tag="PKH68_03865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10768..10770),aa:Arg,seq:tct)
     gene            11102..13546
                     /gene="thrA"
                     /locus_tag="PKH68_03870"
     CDS             11102..13546
                     /gene="thrA"
                     /locus_tag="PKH68_03870"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNX12106.1"
                     /translation="MKVIKFGGTSVGTVEGILSLKNIVESQQEAVIVVVSALSGITDK
                     LYATSKMASEGNPAYLSEFEQMVSRHLELIENVISPEKRTQTIRLVQENFTDLANIFR
                     GIFLIKDISIRITDTIVSYGEVISSIIVSHLIQDAVHFDARNFIKTNNQFDKHIVDFP
                     TTNKLILETFTDAPRVCVCGGFISTDAKTGYITNLGRGGSDYTAAILASALNATKLEI
                     WTDVDGFMTADPKIISNTYTIERLSFSEATELCNFGAKVIYPPTIFPVYHKNIPVEIK
                     NTFNPEATGTYISSDKDTRQKSQQKAIKGISSINDTSLITIQGLGMVGVIGVNYRIFR
                     VLAKNGISVFLVSQASSENTTSIGVRNADSELAVEVLRKEFAQEIIQGEINQIFSEDD
                     LVTIAIVGDNMKRTPGIAGKLFGTLGKNGINVIACAQGASETNISFVTDRAVLRKALN
                     VIHDSFFLSEYQVLNLFIIGVGTVGSSLLSQIKKQQKNLMVYNGLKINVVGIASEKKM
                     IFSRSGVPLENCVERLKKEGQHADTQLICDEILKMNIFNSVFVDCTASAEVANIYQKL
                     LENNISVVTANKIAASSEYEKYVLLKETSRQRGVKFLFETNVGAGLPIINTINNLTNS
                     GDTILKIEAVLSGTLNFIFNTLSCEIPLSKAIRMAKDARYSEPDPRIDLSGKDVIRKL
                     VILAREAGYKINQEDVQKNLFIPDRYFQGNQDDFWKLIPELDADFEEKRKELDKKGLR
                     WRFVATLDKGKCSVGLHAVDSLHPFYDLQGSNNIILISTERYNEFPMIIKGYGAGASV
                     TAAGVFADIISIANIR"
     gene            13546..14082
                     /locus_tag="PKH68_03875"
     CDS             13546..14082
                     /locus_tag="PKH68_03875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX12107.1"
                     /translation="MKTLNHLFPVIIIFCIGLSSCTKPSPQLPSNKTANGNTAIQSML
                     EMNKVLALKEDSTIQAMVDEQAIPYIKSKSGIWYSVIHATNSDSLINDSIVTFSYKMY
                     DLNGTEMLSEKKTVRFGKKEIPTGLEDGLRLMRKGETIRLIVPWYLAYGMKGEDNIPP
                     YTSLMYLIVSEITSPQAH"
     gene            14325..14873
                     /locus_tag="PKH68_03880"
     CDS             14325..14873
                     /locus_tag="PKH68_03880"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HNX12108.1"
                     /translation="MKRFPSIFLITLSLLISISCSNKKTYSELLDEQDATIQAYIQRN
                     SFNVIKITTDTLPANALKGEKDYYLMPSGLYFHIVTEGDKSVKVASRTKVALRYRPYT
                     LTSPADTLANNWNTIASTDPFTVTYGETSTFSVVGIHQAIGLMRYSGSEAKVIVPASL
                     NTGTYSDQVIPVAYDLKIKIVK"
     gene            14957..16348
                     /gene="glmM"
                     /locus_tag="PKH68_03885"
     CDS             14957..16348
                     /gene="glmM"
                     /locus_tag="PKH68_03885"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNX12109.1"
                     /translation="MALIKSISGIRGTIGGRAGEGLSPLDVVKFTSAYAAFILSSKNS
                     ASNTIVVGRDARISGEMVNKLVVGTLLGMGMNVVDIGLATTPTTELAVTMEQAAGGII
                     LTASHNPKQWNALKLLNEKGEFLNAKEGETILKLAENEDFTFSEVDQIGKLTVNDNYT
                     QKHIESVLALDLVDVEAIKAADFKVAIDCVNSVGGIAIPALLDALGVKTVEKLYCEPN
                     GHFPHNPEPLPEHLTEISNVMKSGKFDAGFVVDPDVDRLAIICEDGSMFGEEYTLVAI
                     ADYILRHTPGNTVSNMSSTRALRDVTQKYNCNYEASAVGEVNVVTKMKETNAVFGGEG
                     NGGVIYPASHYGRDALVGIALFLTHLAKEKKTVSELRKTYPNYFMSKNKIELTPQIDV
                     DGVLAVIKGSYKQYEVNDIDGVKIDFPTGWVHLRKSNTEPIIRIYSEAGTPEQAYNFA
                     KAIMEEIKRIAGL"
     gene            16554..>16695
                     /locus_tag="PKH68_03890"
     CDS             16554..>16695
                     /locus_tag="PKH68_03890"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNX12110.1"
                     /translation="MNTIGKLFTFTSFGESHGRGVGGIVDGCPAGIELDEAFIQQELN
                     RRR"
BASE COUNT         5178 a         3525 c         3582 g         4310 t
ORIGIN      
        1 aaaaccactg taatactttc acaccatgat cttatttttc agaacccccg accaattaat
       61 ctatgcagta cagtcaccac aaccactgga tttcgacatc aataagctca tctggttatt
      121 cggagatgct gagttgctga aagaagataa aatttctgga aaatttatcg gaccacgacg
      181 tgagatgata acaccctgga gtaccaatgc cgtggaaatt actcagaata tgggtatcaa
      241 gggcatttta cgaattgaag aattctcacc ggttctggaa aaagatgatc atgaccccat
      301 gctccagcgt atttacactg aactaaatca ggatatattc accatcaaca aactgcctga
      361 accaattcag aacatcgatg atattgctgc ctataacgcc aaagaaggtc tggcactgag
      421 cgatgatgaa atagaatacc tcaacagcat ttccgaaaaa atgggtcgta aactgaccga
      481 cagcgaagta ttcgggtttt cacaggttaa ttcggaacat tgccgccata agatattcaa
      541 cggacagttt gtcatcgatg gcgaagaaaa ggaattatcg ctatttaaac tcataaaaaa
      601 aacctcactc gataatccga ataacctggt ttcggcatac aaagacaacg tggcattcaa
      661 ccggggacct gtcatcgaac aattctcgcc aaaatcggac gaaaaaccgg atttttttga
      721 aacaaaaaat attgaatcag taatttccct caaagccgag acgcataact tccccaccac
      781 tgtggagcca ttcaacggtg cagctaccgg aacgggcggt gaaatccgcg accggctggg
      841 tggtggaaaa gccagcttgc cgattgcagg tacagcggtg tatatgactt cttatccccg
      901 caacgcttcg aagccctggg aaaaaacact gaaagaacga aaatggctct atcagactcc
      961 cgaacaaata ctgattaaag catcgaacgg cgcttccgat ttcggaaata aattcgggca
     1021 accgctcatc tgcggttcgt tactcacatt cgaacatcag gaaaacgaca aaacatacgg
     1081 atacgacaag gtaatcatgc ttgcaggagg tgtaggatat gcttcgcgcc gcgatgcact
     1141 caagggtgaa cctgctcccg gggagaaagt ggtggtgatg ggtggcgaca actaccgaat
     1201 cggtatgggt ggtggtgctg tttcgtcggt tgaaaccggc caatacgcca atgccattga
     1261 actgaatgca gtgcagcgcg ccaatcccga aatgcaaaaa cgggttgcaa acgtaatacg
     1321 tgcccttgcc gaatcggatc ataacccgat tgtttccatt catgatcacg gagccggagg
     1381 ccatctcaac tgcctctcgg agcttgtcga agctaccggt ggagtgattg aaatggacaa
     1441 actgcccgta ggtgatccta cactcagtgc aaaagaaatt atcggtaacg aaagtcagga
     1501 acggatgggc ttgctgctgc ctgaaaatga aatagaacgc attcagcaaa ttgccgaacg
     1561 tgaacgtgca ccattctacg tagtgggcga aacaaccggc gatatgcagt tgacatttaa
     1621 agcccccaca caacctgacc ccattcatct tccgctggaa tacatgatag gcaatccgcc
     1681 ccgcactgtg atcaccgata caagtgtgga agaaaatttt gcatcaccag aaataaatga
     1741 agaacaattg ctcgaataca ttcagcatgt gttgcaaatg gaatccgttg cctgtaaaga
     1801 ctggctgacc aacaaggtgg accgctccgt aaccggtaaa gttgcccggc agcaatgtgt
     1861 gggtgagatt cagttaccgt tgagcgatgt gggtgtcgtt gcactggatt atcgtggaaa
     1921 aaccggcatt gccacctcca tcggacatgc accgctggct gctcttgccg atccggaagc
     1981 cggttcggta ctggctattg ccgaagcgct gacaaatatt gtatgggcac ccctgaaaga
     2041 tggattggat agtgtttcat taagcgccaa ctggatgtgg ccgtgtaaaa atccgggtga
     2101 agatgcccgc ctttacaaag cagtggaagc atgcagcgat tttgcatcgg cgctgggcat
     2161 taatatccct accggaaaag acagtctgtc gatgacacag aaatacgacg ctgaaaaagt
     2221 attttcaccc ggaacagtga ttatctctgc tggagccgag gtatctgaca ttcggcgggt
     2281 agtaagtccg gttctggtca acaatcccgc taccagcctg ttgtatgttg atttttcgtt
     2341 cgacaaacat aaactcggtg gttcggtact ggctcagacc atgaataaaa tcggcaacga
     2401 agtgcccacg attaccgatc ccgaatactt caaggctgct ttcgatacca tacaggaact
     2461 aatcagtaaa aagctgatca ttgccggaca cgatatatca gaaggtggta tgataaccgc
     2521 attactcgaa atgtgttttg caaatgccaa aggtggattg aaaataaatc tggatcacct
     2581 ggccgaacca tcgatgatca atatcctttt tgctgaaaat ccgggtgtga ttattcaggt
     2641 tgagaataaa gctgaagttg aaaaaatact gcgaaacaat ggaataggtt acgccgctat
     2701 agccaaacca atagccgaac gcaggatgga aattcaccgt aagaaaacct cctactcctt
     2761 ctccatcaac gaattgcgcg atcaatggtt cgaagcatca taccttctcg accgccgcca
     2821 aagtggtgaa ttattagcca aagcccgttt cgaaaattat aaaaatcaac agctccaatt
     2881 ccttttccct gctgaattta ccggaaaact atctcaattt gccctctcac ccgaccgcaa
     2941 gccgggaggc gtaaaagcag ccataatccg cgaaaaagga actaacggtg aacgtgaaat
     3001 ggcttatgca ctttatctgg ccggttttga tgtgaaggat gtgcacatga ccgacctcgc
     3061 ttcgggacgt gagacactgg aagatatcaa catgatcgta ttttgcggtg gtttttccaa
     3121 ttccgatgta ctcggttcgg ccaaaggatg ggcaggcggt ttccttttca acgaaaaagc
     3181 gaaagctgca cttgataatt tctacaaacg tccggatacc ctgagccttg gtatctgcaa
     3241 tggctgccag ctaatggtgg aactgaacct gataaatccc gaacatacca acagggctaa
     3301 aatgttgcat aatgattctc acaaattcga atcgggattt gtctccctgt caattcccga
     3361 gaataattca atcatgctcg gatcactttc gggcagcaag ataggtgtct gggtggctca
     3421 cggtgaaggt aagttttcac tccctatggg tgaaaatgca tacaatgtga tagcaaaata
     3481 caactatgag gcttatccgg gcaatccgaa cggctctgat ttttctgttg cagggatttg
     3541 ctctgccgat gggcgtcatc tggctatgat gccgcacccc gaacgggctg ttttcccctg
     3601 gcaatgtgcc aattatccgg ccgaaagact gcataccgat gaaattactc cgtggatgga
     3661 agcatttgta aatgcacgga tatgggtgga agaaagaaca aagtaactat tgattattaa
     3721 ttaaatacgt ttaatttctt ccggattagc tgcatttgct gtttcagatt gaacaaagta
     3781 acttcgccgg aataatatcg ggattatgaa tcaaacttca ctgaaaaaat tcgcctacct
     3841 gtcgatagct gctgctgtta tgactatagg attaaaattc ggagcctatc tgcttaccgg
     3901 ctccatgggc ttattttccg acgcgctgga gtcgtgtgtc aatctgattg cagccgtcat
     3961 tgccctgatt atgattacca tttccgaaag gccggccgat aaagaacatg aatatggaca
     4021 ctcaaaagct gaatattttt caagtgcaat agaaggatcg ctcattttgg tggcagcatt
     4081 cagcattatc tggtcagctg taccccgcat tatcaatcct caaccacttg aaaacgtggg
     4141 aattggtttg ctgatttcaa ctggagcttc ggcgattaac ctggctgttt cactgatact
     4201 gattaaaaac ggcaaaaaaa ggcattcact gctcctggaa gcagatggca aacacctgat
     4261 gaccgatgta tggacttcgg tgggtgtgat agccggtatc ggactggtta aactcaccgg
     4321 actgattatt ttagacccta tcattgctgt tattgttgct ttgaacattg tttggacggg
     4381 tgcacagctg cttcgccggt ccggtaacgg actattagac tcggtgatat caaccgatga
     4441 acaaaagttg attaccgatt ttctggaaac tcaaaaaagt aaaggtatag aatatcactc
     4501 cctgatgacc cggcaggccg gacaacgcat ttttatttcg ttacatctgc tggtgcccgg
     4561 cgcctggaca gtgcagcagg gtcacgacga gtcggattac atcgaggaat acatcgaaaa
     4621 tctattcgaa ggacctgtga cctgctctac acatattgaa cccgtggaag acccggcttc
     4681 actgaacgat ataggcattg accgaaaaag aaaataaaaa agcttctgaa taagttaaaa
     4741 acaaaacgat catcggcctt gttggaattc ttcagaaaac aaaaagccgg caaacattga
     4801 acagaaattt attgaaattt gatgaaaaca gttactcttt tcctgatggc agttgtattg
     4861 aacagtattt cgttgcatgc tgcatccgaa ccgcatttcc tgaaaacgac ctattccaat
     4921 ggagaattta tcactgtttg tcagacagag gtcaatgcgg gtgaaacaca aactaatgcc
     4981 gtgatagcac ggatgattga cgaatttcat accaaaccgg taaatctgtt tgactgggcg
     5041 ctgaaaaacc tgggaatgca gggacagaaa aacaacgaga tgattgtagt gctcaaaact
     5101 tcgaagtttg ataaaaaaac cggcattacc cacggacttg tggatgtgga aataccgtat
     5161 ttacaggatt tcagaaacat aaaaattgac gccgttgtga aaaaggaaaa tcaacccaac
     5221 ggacagttta aagcaacggc cgatgtactc caatcgggtt tactgctgaa aaaagcttat
     5281 ggcactgtta ctgctattcc cgcaaatgaa gatcgtcaaa tattacagac agtaacccgt
     5341 ataaagttcg gatggtttct aaacctgttc atttcacaaa aacgctatca aactatagta
     5401 gaatggcggg tacgtgaatt cacaaaaaac ctgaaactgg aagctgaaaa aacaattata
     5461 gctacaccca attaatttaa caaaaacaag atacgcccct ctgtgggaaa atcagaataa
     5521 ccttatggct gaaaattcat tattagaaaa attagaagga cttcaacaca aatttgaaga
     5581 aatatcgatg ttgattaccg accctgcagt gattgcagac atgaaacggt acataaaact
     5641 caacaaagaa tacaacgaac tgaacaagat aatgacggcg gtgaaagaat ataaaaccgc
     5701 actgaccaat atttcggaag caaaagagat atacgataaa gaatcggacg ccgagttgcg
     5761 cgaaatggca aaagccgaaa tcgacgattt ggaacctcaa attccggtga tggaggaaaa
     5821 tattaaactt ctgctcattc cggccgatcc cgaagacaat aaaaatgtgg taatggaaat
     5881 ccgcggtggc accggtggcg atgaagcggc gatttttgcc ggcgatttgt tcaaaatgta
     5941 cacccgctac tgcgagaata gaggctggaa agtggaagta accgatttca gtgaaggtac
     6001 catcggtggt tacaaggaaa taatatttac agtatcaggt gaaaatgtgt atggaatact
     6061 gaagtacgaa tcaggagtac accgtgtgca gcgggtaccg gtcaccgaga cacagggtcg
     6121 tgttcacact tcagcatcaa cagttgcggt actgcccgaa gccgatgagt tcgaagtgga
     6181 tatcaacccc gctgatattg attttcacac ctcccgctcc ggcggcgcag gcggacaaaa
     6241 tgtaaacaaa gtggaaacca aagtgcaact aacccaccgc cctaccggca tagtggtggt
     6301 ttgccagcag gcacgtaccc agcttggcaa ccgcgaaatt gccatggaaa tgcttcgtac
     6361 taaaatttac aacatggaat atcagaaaca cattgatgcc atagcttcca aacgtaaaac
     6421 gatggtctcc accggcgacc gctcggcaaa aatccgcacc tacaactacc cacagggacg
     6481 tatcaccgat catcgcatca actacaccgt gtacaatctg gtggcattca tgaatggtga
     6541 tatacaggat gtgattgaca agctgataat agccgaaaac gcagaaagaa tgaaggaaag
     6601 cgacctggca taaatggaaa cagtcattaa gtacagcaaa ttaaaacctg ttctgctgct
     6661 gcttgtctca acagcatttg caggtatatg catctggatg ctgatagaaa gtgaggggaa
     6721 aaatctgctg ggttatgcag gttttgcttt cttctttttg ttgtcggttt atatcgttta
     6781 caggctggtg aaaggcggcg gcatcatatt gtctgacaag ggcatcgtta cggaccggaa
     6841 ccatcctgaa gactcgttta taaactggga tgaaatcgaa aaattcacac tcagggaagc
     6901 cgatggcatc aaaaccatca acatacacct gtacaatcct caggtgtttt tagaaaggca
     6961 aaccagaaaa aacgtacgta aaaaaatgga aatcaacgag atattataca aagctccggt
     7021 gggcatcaca cctaatttaa tgaatatttc ctacaaaaga ctggagaatt tgcttataga
     7081 aaaactggaa tcaaatcaga gttcaaaaaa acgtttaaca ccaaataatc atgactaagc
     7141 aagaactttt tgaaaatatt aaacgcaagg aatcctttct ttgtgtgggt ttggataccg
     7201 atatcaacaa aattcccgaa catattctca atatatcgga tgatccgatt tttgatttca
     7261 atgttgaaat catcaaagcc acggctgacc tgtgtgtggc ttataagccc aatctggcat
     7321 tctacgaaag catcggattg gccggctggg atgccctgga acgtacggtg aatttcatac
     7381 gggccgaata tcccgatcag tttatcattg ccgatgccaa gcggggcgat ataggtaaca
     7441 catcggccat gtatgcccgc actttttttg gcaacatgga tgtagatgcc gtgacggtgg
     7501 ctccttacat gggcgaagac agcgtattgc catttttgac atacgaaaac agatgggtca
     7561 ttttgcttgc tttgacctcc aataaagggg cattcgattt tcaatatatg gaggagaatg
     7621 gcgaaaaact attcgaaaaa gtactgaaaa cctctcaaca atgggcttcg gctgaaaaaa
     7681 tgatgtatgt ggtgggtgct accaaagccg aaatgctctc cgatatccga aaaattatcc
     7741 ccaaccattt tttacttgtt cccggtgtgg gagcacaggg tggcagcctc agtgaagtag
     7801 cgcagtacgg tttaaacgat atgtgtggtt tgctggtgaa ttcatcacgt caaatactat
     7861 acgcctcgac cgaagctgac ttcaaagatg cagcacgcaa tgaggccaaa cgacttcaaa
     7921 ttgagatggc tgaaatactg agaagcaacc atttagtata atatatctaa acttttcata
     7981 gatctaatac tcattgcttt ctttgtttta tttttttaat ccaaattact ctgccatgtc
     8041 tgaattttcc acccaccagc gccagcgact tgccgatctg agaattctat tcgatgcaat
     8101 tacgtataaa aagaatgctg cagaagccat aagacagtac caaaaaacca tcgaaaatac
     8161 aattccagat gatgtggtga tattggtaca tcagctcatg gaaagcaact atccgatgga
     8221 ggatatcaaa tcgggaataa acaagatgct caacgtgttg cgccgttcaa tcgatgctta
     8281 cccctacacc tcccccaccg atgatacctt ctttgccgtt tgcattcgca acaacaatga
     8341 agtaaagaat ttactcaact cgcttcggcc ttacatcctg gatatcaata aaaaaggaat
     8401 ttcagatgaa atcacaagcg ggatgctgcg actgtggaaa gaggttcaga aatttacaca
     8461 gtactatatt atcaaggaaa atgtattatt tccccttgtt gaaaaatacc tgcccgaata
     8521 tcggtgcata gccgtgatgt ggtcaattca cgacgatata cgccgtaatc tgagcgattt
     8581 aataaatctg ctttccaccg atgatttaga acttccnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnngccg taatctgagc gatttaataa atctgctttc caccgatgat
     8761 ttagaacttc caaaactgaa ccgactgacc ggtgaagtgt tttacaacat ttacagcatc
     8821 gcgttccgcg aagaacgatt gctttatccg atgatctcga aaaacgtacc tgccgcggaa
     8881 attgaaaaga tgtggaatga aagtgttgaa attggctttc catacattaa tcccgacatg
     8941 aaaacacaac atccggaagt taaaaacgac ccttctgatc tgatagacct gaaaagcgga
     9001 ttgctttctg cagaacaaat attcctcata ttcagccatt tgccggtaga catcacctac
     9061 gtggatgaaa ataacaaagt tcggtttttt tccactccga aaaaaaggat ttttacccga
     9121 acaaatgccg ttttgggtcg cgatgtacgc aactgccatc cacatgaaag tgtacatgtg
     9181 gttgagcaaa tcgtggaatc gtttcgcaat ggcacgaaag ataacgctga tttctggata
     9241 aatatgcggg gtaaaaaaat actcattcag tattttgccg tcagaaatgc tgagaacgaa
     9301 tacaaaggtg taattgaagt aacccaggaa atatccgaaa tacaggcatt ggaaggagaa
     9361 agacgcattc tggactggaa taattagagt atcaacaaaa catttttgtc agtgatgttg
     9421 tttcacaaca tataatagaa attaattcta actgattgcc aaaggaatat acttatgaat
     9481 gattttgtag attatgtgga atatacccga ccggacaatg ctaaagtccg ggtagcattt
     9541 gaaatgcttg gcagcgaagt gctgttttat ttctgcgaat atcagcattc gcccaccgcc
     9601 gaatggaaat tgatacagtc accaaacaac gagaatttca attacaccgc tcccgatttt
     9661 cagggtgaaa tgaggcatta caacatacaa attcccacca aagaagagat agaaaaagcc
     9721 caacaactaa ttgtgaataa gtacaatata ttgagtatct gagatatgtt tcatgtgctt
     9781 gcgaccttta ggcaatgttt ttcatttcaa ttaaacttct atgtttttaa atgaacaaaa
     9841 taaacataac tgatgtttaa aattcataat agcacagaag tcattcatta tgaattttct
     9901 gcgttatttt taagttgata ttttactgat tggataaaaa gacatcacat acatactgct
     9961 atttatcaag taattaactt ataaatcact cagccatgaa aatcctgtcc aatttattct
    10021 gcacactatc tgtgcttttt ttcttttctt cctgcgatga gatcttaaag cccggaacaa
    10081 cattgggtgg ttcacagtca ccgatgggtg aagtcggggt tatcgttacc tccagttcgg
    10141 ctcccatagc cggtgttagt ggtttttcag cctctgttac ctctctcacc gatggggtca
    10201 gcacatacag tgctcaggct acagtgacca atgctttact taaaaatatg gtatcggatt
    10261 tcccgggagt caccgtaaat ggcaacaccg ttacaattac cggtttgaag atacagcaaa
    10321 caaccgaagg cattaaatgc ctgacaggcg atggtgccgg gataattgtg aagtattcat
    10381 cagcggtagg cgatacctat ccagtgggcg atacaggcaa taaacggaaa gtggttagta
    10441 aatccaccga tgatgattat ccctacggat tttacaatat caaaaccatt caggtggaaa
    10501 ccgatgccaa caacctcgga agtaccggtg gtattgacaa aatcacctac atagccaatc
    10561 acaaattcgg acttgttgga gtaaaaataa catttaatga tgctaccagt gtcaccttcc
    10621 cggtttacac caatgctcag aattagcctg gaaatcagta aacggttaaa tatttgaaag
    10681 acttattgaa aaaaaataaa ctgctgataa ttagcagttt attttttttg tgatcccggc
    10741 gggattcgaa cccacgaccc acagcttaga aggctgttgc tctatccagc tgagctacga
    10801 gaccatttac aaaagtggtg caaaagtaag ccaattttcc cgactttgca atacttttcc
    10861 ttaaaataac ttccaaatca tctgtaattg ccaaaaaatg agtaatattg cagtcggaaa
    10921 tctttttgac accctaaagg tgtttttttt cggtttttat ttccgaaaac ctactttttt
    10981 aatcagattt taagcatttt atccttttgt ctgaaatcaa cgaaaggatt tttcattaaa
    11041 aacaactatt ttgtgttgaa atccattcgg aattttaata cgctatttaa tttttttaag
    11101 gatgaaagtt atcaaattcg gaggaacttc agtcggtacg gtcgaaggta ttttgagctt
    11161 aaagaatata gtagaatcac agcaagaggc tgtgatagtg gtcgtttcag ctttgtcggg
    11221 cattaccgat aagctttacg ctacctccaa aatggcttca gaaggcaatc ctgcctatct
    11281 gtcggaattc gagcaaatgg tatcgcgtca tttagaattg attgagaatg tcatttcacc
    11341 cgagaaaaga acgcaaacca tcagactggt tcaggaaaac ttcaccgatc tggccaatat
    11401 atttcgcggc atatttctga ttaaggatat ttccatccgc atcaccgata ccattgtgag
    11461 ctacggtgag gttatttcat ccattattgt gagccatctg atacaggatg cagtgcactt
    11521 tgatgcccgc aattttatca aaacaaacaa tcagtttgac aaacacatcg tcgattttcc
    11581 cactaccaat aaacttatac tggaaacgtt taccgatgcc ccccgggttt gtgtatgcgg
    11641 cggatttatt tccaccgatg ccaaaaccgg ttatattact aatttggggc gtggtggctc
    11701 cgattataca gcagccatcc ttgcatcggc acttaatgcc acaaaactcg aaatctggac
    11761 ggatgtggac ggatttatga ctgccgatcc aaaaatcatc agcaacacat atactattga
    11821 aagactgagc tttagcgaag caacagaact ctgtaatttt ggtgccaagg tgatttatcc
    11881 acccaccatt tttccggtct atcacaaaaa cattccggtt gaaatcaaaa acactttcaa
    11941 tcccgaagct accggaacat atatttcgag cgacaaagac acacgacaga aaagtcagca
    12001 aaaagccatt aaaggaattt cctccatcaa cgacacatca ctcatcacca ttcagggact
    12061 ggggatggtg ggcgttatcg gggtcaatta ccgcattttc cgtgtgttgg ccaaaaacgg
    12121 tatcagcgtg ttcctggtat cgcaggcatc gtccgaaaac accacctcca tcggggtgcg
    12181 aaatgccgat agtgaattgg cagtagaagt acttcgcaaa gaatttgcgc aggaaatcat
    12241 tcagggagaa atcaatcaga tattctccga agacgatttg gtgaccattg ccattgttgg
    12301 tgataatatg aagcgtactc cgggtattgc cggtaaactt ttcggtacac tcggtaaaaa
    12361 tggtatcaat gtgatagcct gtgctcaggg agcatcagaa accaacattt cgtttgtaac
    12421 tgacagagct gtactgcgta aagcgttaaa tgttatccac gattcattct ttctttcaga
    12481 atatcaggtt ctcaatttgt ttattatcgg tgtgggtacg gtgggcagca gcctgttgag
    12541 ccagattaag aaacaacaaa aaaatctgat ggtttataat ggcctgaaaa ttaatgttgt
    12601 gggcattgct tcggaaaaga aaatgatttt ttcacgcagt ggagttccgc tggaaaattg
    12661 tgtggaaagg ttgaaaaaag aaggtcagca cgctgataca cagttgatat gcgacgaaat
    12721 attgaaaatg aacatcttta attcggtttt tgtggattgt acagccagtg ccgaagtggc
    12781 aaatatctat cagaaattac tggaaaataa tatttcggtg gttactgcca ataaaattgc
    12841 tgcatcgtcg gaatacgaaa aatatgtgct gttgaaagaa acatcgcgtc agcggggtgt
    12901 caaatttctc ttcgaaacca atgtaggagc cggattaccc atcatcaata cgatcaacaa
    12961 cctgaccaac agcggtgata caatcctcaa aattgaagcc gtactttcag gaacactgaa
    13021 ctttatattc aatacactga gttgcgaaat tccattgagt aaagccatcc gcatggccaa
    13081 agatgcccgc tactccgaac ccgatccgcg cattgacctg agcggcaagg atgttatccg
    13141 taaactggtg atactggcac gtgaagccgg ctacaaaatc aatcaggagg acgtgcagaa
    13201 aaacctcttt atccccgacc gttattttca gggtaatcag gatgatttct ggaaattgat
    13261 acccgaactg gatgccgatt ttgaagaaaa acgaaaggaa cttgataaaa aaggattgcg
    13321 ttggcgattt gtagctacgc tggataaagg aaaatgcagc gtagggctgc atgcagtaga
    13381 tagtttgcac ccattctacg atcttcaggg aagcaacaac attatactga tatctaccga
    13441 gcgatacaac gaatttccga tgattatcaa aggctacggt gccggggcaa gtgtaactgc
    13501 tgccggagtt tttgccgaca ttatcagtat agcaaatatc agataatgaa gacattaaat
    13561 catttatttc cggtaataat tattttctgt attggattaa gttcctgtac caaaccttcg
    13621 cctcagctcc catccaataa aactgccaat gggaatactg ccatccaatc gatgcttgaa
    13681 atgaataaag tgctggcttt gaaggaagac agtaccattc aggcaatggt tgacgaacaa
    13741 gccattccct acataaaatc aaaatcaggc atttggtata gtgtaattca cgccaccaat
    13801 tccgattcgt tgataaacga ctccattgtc accttcagct ataaaatgta cgacctgaac
    13861 ggaacagaaa tgctaagtga aaagaaaacc gtccggtttg gaaaaaaaga aattcctacc
    13921 gggctggaag atggtcttag acttatgcga aaaggtgaaa ctatacggtt aattgtgccc
    13981 tggtatctgg cctacggaat gaaaggagag gataatattc caccatacac ttcgcttatg
    14041 tatttgattg tgagcgaaat taccagccca caggcccatt gaaggaggag ttgcagagtt
    14101 ttaagtgaag taaacccctg cttttctacg gagttaatat tttcaaataa aggtccgata
    14161 tccacttgat agcaggtaac cggcatcata taaatgccag ctatatagtt acatgtgaat
    14221 taaaaattgc ttgttactaa aatttaactt ttgtatatca ctataaaatc ggatatttgc
    14281 attacatatt ctctattaaa aataagccag acttcccaca aaaaatgaaa agatttccct
    14341 caatatttct gatcacatta tcactgctga tttcgatttc ctgctcaaat aaaaagacgt
    14401 attccgagtt gcttgatgaa caggatgcca ccatacaggc atacattcaa cgcaacagct
    14461 ttaacgttat aaaaataacc accgatacgc tccctgctaa tgcgctgaaa ggtgaaaaag
    14521 actattacct tatgccttcg ggattgtatt ttcatatagt aactgaaggc gacaaatcgg
    14581 ttaaagtagc cagtcgtacg aaagtagccc tccgctacag accgtatacg ctcaccagtc
    14641 cggccgacac gttagctaat aactggaata caatagccag caccgacccg ttcactgtaa
    14701 cttatggtga aacttccact ttttcggtag tcggaattca tcaggcaata ggactaatgc
    14761 gatattccgg ctcggaagca aaagtgattg tgcctgccag tttgaatacc ggtacgtatt
    14821 ccgatcaggt cataccggtg gcgtatgatt tgaaaataaa aattgtaaaa taaaacactg
    14881 ataaatatgc cccttttcta aagactgggg cattttcttt tatatatttt tttaacagaa
    14941 catttaaaga tactgtatgg cacttatcaa atcaatttca ggtatcagag gaaccatcgg
    15001 cggacgtgcc ggagaaggat tgagtcctct cgacgttgtg aagtttactt cagcttatgc
    15061 tgcatttatt ttatcgtcaa agaattcagc ttcaaacacc attgttgttg gccgagatgc
    15121 ccgcatttcg ggcgaaatgg tgaataaact ggttgtagga acgctattgg gaatgggaat
    15181 gaatgtggtt gatatcggac tggctacaac accgactacc gaacttgctg taaccatgga
    15241 acaggcagcc ggcggcatta ttcttacagc cagccacaac cccaaacaat ggaacgccct
    15301 gaaactgctg aacgaaaaag gagaatttct gaacgccaaa gaaggtgaaa ctattcttaa
    15361 actggctgaa aacgaagatt ttacattctc tgaagtggat caaatcggaa aactcaccgt
    15421 aaatgacaat tatactcaaa aacacatcga aagcgtactg gcacttgatc tggttgacgt
    15481 ggaagccata aaagcggctg atttcaaagt agccattgat tgcgttaatt cggtgggcgg
    15541 aattgctatt cctgctttgc tcgatgcatt gggagtgaaa acggtggaaa aactctactg
    15601 tgaaccgaac ggacactttc ctcacaatcc cgaaccccta cccgagcacc ttacggagat
    15661 ttcaaacgta atgaaaagcg gaaaattcga cgcaggcttt gtagttgatc ccgacgtgga
    15721 tcgtctggca ataatctgtg aagatggaag catgtttggc gaagaatata cactggtggc
    15781 aattgccgat tatattttac gacacacacc cggaaatacg gtttcgaaca tgagttccac
    15841 ccgtgccctg cgcgatgtta ctcagaaata caactgtaat tacgaagcat ctgctgtagg
    15901 cgaagtgaat gtggtgacca aaatgaaaga aaccaacgcc gttttcggcg gcgaaggcaa
    15961 cggcggtgtt atttatccgg caagccacta cggtcgcgat gcactggtgg gaattgcgct
    16021 ctttttaaca catttggcaa aggagaaaaa aaccgtttca gagcttcgca aaacctatcc
    16081 caactatttt atgagcaaaa ataaaataga actcactccg cagattgatg tggatggtgt
    16141 gctggcggta ataaaaggca gctacaaaca atatgaagta aacgatatcg acggagtgaa
    16201 aattgacttc cctacaggct gggtacatct tcgcaaatcg aacacagaac cgattatccg
    16261 catttattcc gaagccggaa cacccgaaca ggcttataat tttgccaaag cgattatgga
    16321 agaaattaag aggattgcag gtttataacc accccctaaa tcccctgaag tgggactata
    16381 aaaacgccac attttttgtg gcgtttttgt tttaacttga ttttttcttc aatttctacg
    16441 ttacaaatga atggaaataa caatcgattt acttttaatc aaattttatt ttaactttgc
    16501 ataagatata actaactaca tagaactccc cctttagggg gttgggggca attatgaata
    16561 caataggcaa actctttaca tttacatcat tcggcgagtc gcacggacga ggcgtcggcg
    16621 gcattgtgga cggttgtccg gccggaattg agctggatga ggcatttatc caacaggaac
    16681 ttaaccgccg ccgtc
//
LOCUS       DAOPZK010000039        16585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3691_length_16585_cov_18.095342, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000039 DAOPZK010000000
VERSION     DAOPZK010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3691_length_16585_cov_18.095342"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKH68_03895"
     CDS             <1..683
                     /locus_tag="PKH68_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12111.1"
                     /translation="EAMDDIKPLLTMDVIGQNSSDRVYLITAEGTTKGYDPGWDGTKS
                     LSTDMVQIFALDADNRRMQVNTDNDLNDTYIGFRTGGESMYSLRFKFNNEMQGLYQSL
                     YVQDLATGTTQEITDGTTMSFASTAGTAEKRFRILAARIITGSGTIEGKETISVMTSS
                     SAIRVNNNTNDEVSLKVFNLAGQLLLLDKAAVGVQTINHHLTKGTYIIEAKSVHSGSR
                     TTVKAIIH"
     gene            795..1439
                     /locus_tag="PKH68_03900"
     CDS             795..1439
                     /locus_tag="PKH68_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12112.1"
                     /translation="MKTKIRAISLMTLGVLILSFFVIRAVLQVMTEEAKNPYKVYQGT
                     ATPTYNTNAYVMKVPAHKSGGIAVNHHRQSGRSVARQSTPNVYVPLKSVEQGISSGFS
                     LPATNNAIYSRRSKKSADDNSMNNQTLTVMPVRITKPSGTMNLVTTGKDVQGDLVASN
                     MSAPFSDGNISGPMRMEGEGDNPPPEGMPVGNGVWVMLFLAAGYILYRRKFAAH"
     gene            complement(1636..2901)
                     /gene="xseA"
                     /locus_tag="PKH68_03905"
     CDS             complement(1636..2901)
                     /gene="xseA"
                     /locus_tag="PKH68_03905"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNX12113.1"
                     /translation="MNSVSLSELTDRIQGVIRANFETSVWVRAEISELRENNGHCYLE
                     LIEKDADTDGILAKTRATCWASVFRMLKPYFESSTGESLRAGLNVLVAVTVEFSGLYG
                     FSLNVRDIDPSFTIGELAARRLQIIRQLEADGIADMNKQLPFPHLPQRLAIISSATAA
                     GYGDFCDQLKRDSSQFAFYPKLFPATMQGEQAEASIIAALERIYKHMDLFDVVVIIRG
                     GGATTDLACFDTYNLALNCAQFPLPVIAGIGHQRDISILDMVAHTSVKTPTAAAEFLI
                     GKMQDAKDELLNLADDIHSVISQLISTQEQQLERMKLQIEQAARTGYLRQQHQLEQQG
                     YRIRTAVRQIMQNHQIKLEQLRNRIDIHSPAFLLQRGYSITTLNGKRLTSVVQVKPGN
                     VIKTWLADGEFESEVGSPPTPQKGGIKRL"
     gene            complement(2898..4544)
                     /locus_tag="PKH68_03910"
     CDS             complement(2898..4544)
                     /locus_tag="PKH68_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX12114.1"
                     /translation="MKKITRNIYLLFLLLFLFGAATADATGYYFYVQLGDKSNSPYSI
                     SHPEAYLSARALERRAYYFIDVDSTDLPVSPVYEQQIRDLGIHIHCRTKWLNGLTVLV
                     ADSSVMSQVRGLPFVKRVQFTGITNENTAAGSRRRISKAVNFNYGAAATQINQLNGSI
                     LHDSGFRGENIHIAVLDAGFLNVDLNPAFDSLRMENRLLGTKDFVNPASNIYDEDPHG
                     AYVLSSMTGNIPGKYIGTAPRASYLLLRTEADEGEYLCEPDFWVSGIEYADSAGVDLA
                     TTSLGYTQFDDSTMNYTYADMDGKTARASIAATMAFRKGIMLLNSAGNDGGYDWHYIG
                     VPADADGVITVGSVTKTGTPSYFSSFGPSSDGRVKPELCAMGSSSSLVSYADSVVING
                     SGTSFACPILAGMTACYLQAAKAKAPSHTLSLLRRYLTESGNLYNAPTDQMGYGIPDF
                     AKAYTAINNTSVENIKNELHVKLLVNSTERKLKIRILDSTLNLNKAMLVLYSISGQAV
                     LSVPIKFRSSDCDIAALRSGIYLLTIEESGIKYRNNILIR"
     gene            5368..5739
                     /locus_tag="PKH68_03915"
     CDS             5368..5739
                     /locus_tag="PKH68_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12115.1"
                     /translation="MKKDWKRKIYGGLWLTSIAFVFQACYGTPQDFGNDLLLTGQVNS
                     KKTGLPVKGIKVSVVDGIQYIKTDDAGKFSLYIGKTESIRLKFEDVDADANGTFQTRD
                     TLLTTSKDVDEINLAIQLEEK"
     gene            5740..6801
                     /locus_tag="PKH68_03920"
     CDS             5740..6801
                     /locus_tag="PKH68_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HNX12116.1"
                     /translation="MNASLQQFIFRQFRKSETKRHELSYLFWECTTRCNLNCRHCGSD
                     CSKDSLFKDMPLTDFLKALDTIPSPSENFTVVLTGGEPLLRTDIEHCGREIRKRGMRW
                     SMVSNGYLYDEAKHIALLNAGLGALTISVDGLEQSHNWLRNNNKSFERVDRAIGLAAG
                     TPRLNFDVVTCVNQRNFHELEKIRKYLISKGVKAWRLFTIIPIGRAKDDAEMFLTDNQ
                     MKELMEFISRSRKKEDIEVKFSCEGYVGNYEPKVRDTPFFCRAGINIGSILIDGSISA
                     CPNIDRTLAQGNIYRDNFFTVWQTKYQLFRNRSWTRTGKCADCKDYRDCRGNGLHYWH
                     GDRTNPLVCHNEQIKNATI"
     gene            complement(6962..7612)
                     /locus_tag="PKH68_03925"
     CDS             complement(6962..7612)
                     /locus_tag="PKH68_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNX12117.1"
                     /translation="METKHQVHNLILLDESGSMTSIKNTIIQGFNEIVQTVKGIALQY
                     PEQEHLITFVTFNGLGMKTLHFCEPVEKLNEIDDEKYQPDAMTPLYDAMGFSFAKLRK
                     ELEGKSEYNVLVTILTDGEENSSKEFSGATIKQLVEELSQKNWTFTYIGADHDVEKFA
                     VSISITNTMQFNKNEADMKQMFVKEQNARMRYSQKIHTKENTSMSFYDEEEDPKKS"
     gene            complement(7686..8597)
                     /locus_tag="PKH68_03930"
     CDS             complement(7686..8597)
                     /locus_tag="PKH68_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNX12118.1"
                     /translation="MDQPKLERLLRVMKMLTANNSLTVDEIADQLSISPRSVYRYIDT
                     FREAGFVIKKKNNCIKLDKSSPYFKDISDLLHFSPEEAYILKEAIESIDENNVLKQNL
                     KKKLYTVYNYNILAETIVNGKNGRNVERLVDAIESRRPVILRNYSSAHGNDIRDRCVE
                     AFAFTTNYVQVWCYCPDENCNKLFKVSRIGSVDIQPGYWKHTRKHKTGKIDIFRMNSS
                     ETFHITLKLGLRAMNLLVEEFPLAAPQLRKLPGNQWLLDTDVCSPEGVGRFVMGLPED
                     IEIINSPELRDYIVNNLVKMKNKYFVS"
     gene            complement(8937..10946)
                     /locus_tag="PKH68_03935"
     CDS             complement(8937..10946)
                     /locus_tag="PKH68_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX12119.1"
                     /translation="MKLVKLNKKLLSAAGLLAVLITFNSCNEFLDRPPLSQVGPGVFL
                     NSEADLASYPLSYYGSVFPSHGGWGVGIGNNDNGTDNQATANPNLNLYTKGTWLVPND
                     GDLGFGTIRGCNYFLEQVLPKWKDGKISGNSQNINHYVGEIYMIRAMAYFEKLKTYGD
                     FPIVKTTLPDQGDVLIAAAKRAPRNLVARQILSDLDSAILLLSSSSFNKVRITKEAAL
                     IAKSRVALYEGSFLNYHKGTPRVPGGPGWPGASMEYNKDFKIEIDTEINFFLDQAMAA
                     AKEVADKTALTANSGVTNPPARDKFSGWNQYFEMFGSKDLSKFAEVAMWRQYNLGLSV
                     THGVTIYVERGGNTGYTKSLVDSYLMKNGLPWYAAGSGYQGDVKIMDVKKDRDLRLQL
                     FLAGEEDVRTVKKDADLFGRPSIINIPETRDVTGYRSRKYLNYDPTEAPGSDLTCVSG
                     SIVFRASEAYLNYIEACYIRKKSIDGDADKYWKALRTRAGVDTDYTKTIAATDMDKEK
                     SDWGAYSAGNMVDATLYNIRRERRNELASEGFRFNDVVRWRAMDQVSNYIVEGFNLWD
                     EAYNDKVYTDPKPGEVTSTGLIADGSSKANVSNKSLSKYLRPYQIVNLSTNLVFNGYN
                     WSKANYLSPMPYRQMQLASPTGDAATSNLYQNPYWPSEPNAAAIE"
     gene            complement(10959..14171)
                     /locus_tag="PKH68_03940"
     CDS             complement(10959..14171)
                     /locus_tag="PKH68_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX12120.1"
                     /translation="MNKQKILIHWRSILTILMCAITLSGYAQTKTVTGTVVDKQNEPL
                     IGVTVKVQGTTNGTVSNFNGVYTLNNVPQNANLELSYVGMKTQTINVSGQSVVNVTMD
                     EDTEMLEEVVVVGYGTQKKVNVTGAVSTVSSKELESRPVQNVSQALQGIVPGLNLSQT
                     NSGGALNSSMSVNIRGTGSIGDGSAASPLILIDGVEGNMNALNPNDIESISVLKDAAS
                     SSIYGARASFGVILITTKSGKTGKTRVSYGGNLRYSNAVQIPEMLDSYRFAQYFNQAA
                     INDGQGAVFSDKALQNILDYQAYQNGTYTGTLTDENRNQIMYGTTADSNNRWLMYGGA
                     NASTNWFKEMYSDWAPAQEHNVNVSGGTDKVTFLVSGNLLDQKGLIRHGEDKFNRYSL
                     NGKMSIKMAPWATLDYNNRWIRETYSRPAYMTGLFFHNIARRWPTNPVYDPNGHYMEG
                     NEILQMEGGGLDKNEKDYLNQQLKLSLEPLSDWFVRLEGSYNTTINFNHWDKLPIYGY
                     DAKNQPFAAPWEGGDAGQSAVGESTYKYNFWSVNLYTDYSKSINDHNFKVLAGTNIEL
                     MKTRTLGGSKKDLITPLIPTINTATNSTPSLSGGYDQWSTVGFFGRLNYNYKEKYLLE
                     ANLRHDGSSRFINDKMWGTFPSFSAGWNIANEDFFKNLNTPISTLKLRASWGQLGNMN
                     TTQWHPWFLSMPISTGSGTWLVGNVKPNTSSAPGIVSSLMTWETIQSSNIGLDWNALN
                     GRFTGSFELFNRRTLNMIGQAIELSSILGTSVPKLNNADMESKGWELELNWRDRISDL
                     SYGVKFVLSDDRQQILRFPNPTKALSNWYEGQVVGEIWGYTTAGIAKSNEEMINHLAT
                     NKPSWGSNWAAGDVMYKDLNDDQKVNSGKSTLDDHGDLSVIGNSNPRFKYGFTLDVAY
                     KGFDFRAFLQGVAKRDYMLGGPYFWGASGGMWQSAAFTQHWDFFRPEGDPLGANLDAY
                     YPRPQFSQSSKNQQTQTRYLQNAAYMRLKNIQLGYTLPASIVKYAAMQSARIYVSADN
                     LLTVSQITGIFDPELLGGDWGQGKLYPLSRTVSFGVNLNF"
     gene            complement(14581..15792)
                     /locus_tag="PKH68_03945"
     CDS             complement(14581..15792)
                     /locus_tag="PKH68_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNX12121.1"
                     /translation="MTVKDKFLKLEQNTKSSQIKKEIISYFLNYGKTSISELAKEMDL
                     SVPTVTKLINNMSDDGLLSDYGKMPTPEGRHPNFYGLNPTSAYFMGIDMTRDYLNMAL
                     MDFSGDIVKIKMNIRYNYENTLESLDDLCTKVEEFINEEPVEIRNNIFNINLNIAGRV
                     NPESGYSYSSFYFSEEPISSILSKKLNRNVGIDNDTRAMTYGEYMKGIVTGQKNVLLI
                     NYSWGLGIGIIIDGKPYYGKSGFSGEFGHFPAFDNEMICHCGKKGCLETEASGFAIYR
                     TVLDRIKKGESSILSDRVDDVNNLTLTDIIDATNQEDTLCIEVVEAVSAKLGKYIAGL
                     INIFNPELVVIGGQVADTGGFVLLPIRSAIRKYSLNLVNKDSQIELSKLKNKAGVYGA
                     CLLGRNRLLSN"
BASE COUNT         4690 a         3482 c         3457 g         4956 t
ORIGIN      
        1 cagaagcaat ggatgatata aagccgctgc ttactatgga tgtgattggc caaaatagtt
       61 cggacagggt atatctgatt actgccgaag gcactaccaa aggctacgat ccgggttggg
      121 atggaacaaa atcattatcc acagatatgg tgcagatttt tgccctggat gccgacaaca
      181 gacgcatgca ggtcaatacc gacaatgatc tgaacgacac ctatataggt ttccgtaccg
      241 gcggcgaaag catgtactcg ctgcggttca aattcaacaa tgagatgcag ggcttatacc
      301 aatcactcta tgtgcaggat ctggctacag gaactacgca ggaaataacc gatggcacga
      361 ctatgagctt tgcatcaaca gcaggcacag ccgagaaacg tttcagaata ttggctgcaa
      421 gaataataac cggttccggg actatcgaag ggaaagaaac aataagtgtg atgacctctt
      481 cttctgcaat cagagtgaat aataatacga atgacgaagt atcactgaaa gtgttcaatt
      541 tggcaggaca actcttgctg ctggataaag cagctgtggg cgttcaaacc ataaatcatc
      601 acctgactaa aggtacgtat attattgaag ctaagtcggt tcactccggc agcagaacaa
      661 ccgtaaaggc aataattcat taactaacgt cataataatc cttgctcaaa acccaatgat
      721 caaatcaatt acatatctct ataccgggac attggacgta aaggcataag tgataacaac
      781 taaaatataa agcaatgaaa acaaaaataa gagctataag tttaatgacg ttaggtgtct
      841 taatattgtc atttttcgtg ataagagccg tgcttcaggt gatgaccgaa gaggcaaaga
      901 acccctacaa ggtgtatcag ggcactgcaa ctccaacgta taacaccaat gcttatgtga
      961 tgaaagtgcc ggctcacaaa tcgggaggta tcgctgtaaa tcatcacaga cagtcgggca
     1021 gatcggtcgc caggcagagt acacccaatg tgtatgtgcc cctgaagagt gtcgaacagg
     1081 gaatttcttc cggattttct ttgcctgcta ccaataatgc catttactca cgaagatcaa
     1141 aaaaatcggc agacgacaat tcgatgaata atcaaacgct gacagtaatg cctgtgcgga
     1201 ttacaaagcc atccggcacc atgaacctgg ttaccacggg taaagatgta caaggcgatc
     1261 ttgtagcctc caatatgtct gctcctttct ccgatggtaa tatctccggt cctatgagaa
     1321 tggaaggtga aggtgataat cctccacccg aaggaatgcc tgtaggcaac ggcgtgtggg
     1381 taatgctctt cctagcagct ggttacatcc tttaccgcag gaaatttgct gctcattaat
     1441 cactgtttaa ttactgatta aaccctaatt acaaacataa caggtgtccg ggcttcttcc
     1501 ggaaacctgt ttctttttag aaacgaaata ccaatttgaa aatgtgtcaa tttgaaaatg
     1561 tgctaatatg aagatttgag accttaatga tgtgcattct ccttaaatta gtttacagta
     1621 ttgaagaatt gtttgctata aacgttttat accccccttt tggggggttg gggggcttcc
     1681 cacttcgctc tcaaattctc catctgccag ccacgtcttt atcacgttcc cgggtttcac
     1741 ctgaactact gatgtcagcc gtttcccatt cagggtggtg atactgtagc cccgctgaag
     1801 taagaaagcg ggcgaatgga tatcaatccg gttgcgtagc tgctccagtt ttatttgatg
     1861 gttctgcatg atttgtctca ctgccgtacg tatccggtaa ccttgctgct caagctggtg
     1921 ctgttgtctc aggtagccgg ttcgggcagc ctgctctatt tgcagtttca tgcgttcgag
     1981 ttgttgttcc tgtgtactta tcagctggct aatgacggaa tgtatatcat cggccaggtt
     2041 caggagttca tctttggcat cctgcatttt gcctataaga aattcggctg ctgcagttgg
     2101 tgtttttaca ctggtgtgtg ccaccatatc cagtatggaa atgtcgcgct gatgtccgat
     2161 accggctatc accggaagtg gaaactgcgc acagttcagt gccaggttat atgtgtcgaa
     2221 gcaggccaaa tcggttgtgg ctccaccacc acggatgatt accaccacat cgaaaaggtc
     2281 catgtgttta taaattcttt ccagtgcagc tatgatggaa gcttcagcct gctcgccctg
     2341 catggttgcg gggaagagtt tcgggtaaaa ggcaaattgg gaactatctc ttttcaactg
     2401 gtcacaaaaa tcgccgtaac ccgcagctgt agccgatgaa ataatggcta accgttgtgg
     2461 gagatgtggg aaaggaagct gcttattcat atcggcaatg ccgtcggctt ccagctgtcg
     2521 gattatttgc agccggcggg ctgccagttc gccaatggta aatgaaggat caatgtcgcg
     2581 cacattgaga ctgaaaccgt aaaggccact aaattcaaca gtaaccgcta ccagtacatt
     2641 cagtcctgcc cgcaggctct caccggtact gctttcgaag tagggtttta gcatccggaa
     2701 tacacttgcc cagcaggtag cccgtgtttt cgccagaata ccatccgtat cggcatcttt
     2761 ctctatcagt tccaggtagc aatgcccgtt gttttcacgc agttcgctta tttctgcacg
     2821 tacccaaact gacgtttcga aatttgccct gattacaccc tgtatccgat cggtaagttc
     2881 tgagagagat actgagttca tcgtattaaa atattattcc ggtattttat tccgctttct
     2941 tctattgtaa gcagatagat accactcctc aaagcagcaa tatcgcaatc ggatgaccgg
     3001 aacttaatgg gcacgctgag tactgcctgc ccgctgatgg aataaagtac cagcatagct
     3061 ttgtttagat tcaatgtact gtccagtatt ctgattttga gttttctttc ggttgagtta
     3121 accaacagtt ttacgtgcaa ctcattttta atattttcca ccgaagtatt attaatggct
     3181 gtgtacgctt ttgcaaagtc cggaatgccg taacccatct gatcggtagg ggcattgtaa
     3241 agatttccac tttctgtcag gtacctacgc agcagcgata atgtatgtga aggcgccttt
     3301 gctttggcag cctgcagata gcaggcagtc attccggcta atatggggca tgcaaaggat
     3361 gtgccgctcc cattaataac aacactgtcg gcataagaaa ctagtgaaga agaggaaccc
     3421 atggcgcaga gttcgggctt aacgcgtccg tctgagcttg gaccgaagga tgagaagtaa
     3481 cttggtgttc cggttttggt cactgatccc actgtgataa ctccgtcggc atcggcaggt
     3541 acgccaatgt aatgccagtc gtaaccaccg tcattgccgg cactgttgag caacatgata
     3601 ccttttctga atgccatcgt tgctgcaata cttgcccggg ctgttttgcc atccatatca
     3661 gcgtaggtgt aattcatcgt actgtcgtcg aattgggtat atccgagcga ggtggtggcg
     3721 agatccactc ctgcactgtc ggcatattct atacccgaaa cccagaaatc gggttcgcac
     3781 agatactcac cttcatcagc ttcggtgcgc aggagcagat aggaagctcg tggagcggtt
     3841 ccaatgtatt tgccgggaat gttgcccgtc atcgacgaaa ggacatacgc accatgagga
     3901 tcttcatcgt atatattgga ggcgggattt acaaaatctt tggtgcctaa cagccggttt
     3961 tccatacgca ggctgtcgaa tgcggggtta aggtcaacat tcaggaaacc ggcatcgagc
     4021 accgcaatat gaatgttttc tcccctgaaa ccgctgtcat gcagtatgct gccattcagt
     4081 tggttgattt gtgtggcggc tgcgccatag ttaaaattca cagccttaga tatccttctg
     4141 cggcttcctg cagcagtatt ctcattggta atgccggtga attgcaccct tttcacaaaa
     4201 ggcaaccccc tcacctgtga cataacgctg ctatccgcca cgagtacagt cagaccattg
     4261 agccactttg tacggcaatg aatatgaata ccaagatctc taatctgctg ctcatacacc
     4321 ggacttaccg gaagatcggt actgtccaca tcaataaaat aataagccct gcgctccagt
     4381 gcacgcgctg ataaataggc ctcaggatga ctgatggagt aaggcgaatt ggatttatca
     4441 ccgagctgga cataaaaata gtaaccggtg gcgtcggcag ttgccgcacc aaacagaaat
     4501 agcagtaaaa acagcaggta tatattacgg gtaatttttt tcattgcgat aacgttcaaa
     4561 taatcataaa gtaacgaatg acaaaaataa gcatttattt cttttattga tgaatttcga
     4621 taccggaaac cgaatatcgg attgatatgc taattattgg tatcaggttg ttgctggtta
     4681 ttattaatgt aatagttcga caacatccct atagccgacg aaaattcatt gtaattattg
     4741 ccggttttat tacgttccaa tgcatgaaat ggcaatatta cgacattaag tgcccgtttt
     4801 ttgacatgaa gtgcgttaaa agggtaatga aattttttat ttattaaaaa aaacagcata
     4861 tttcatttgg aattgagaaa aattacatta actttgtatt gtaaacaaac agataaattt
     4921 ttctaaacaa agatttaggt taaaattggg caatgggtga ctgtgaagtt atccattgtt
     4981 ttttttaata agtttcacat tcaccccacc aactgtcatc aatcccattt gtacgaaaac
     5041 agaatactta aacgttcttc caataatcaa atggaaagtt gtgttaaata ttcttttaaa
     5101 tagaactaat ttaccgattt attttggtat ctttgagtgt gataagattt aaaatctaca
     5161 ggtaattttt tttacatacg aatacaatac aagcaaaata ttcgttcact gaatttattg
     5221 aataaaaaaa ttaacccaat gaaacaccca tgtccaacta aaaattttgg acacacaaag
     5281 gcatgattaa ataacgcata acaaatgaga gacgctcaat attccccctt tagggggtta
     5341 ggggccgata agtaaattaa taacccaatg aaaaaagact ggaaaaggaa aatctacggt
     5401 ggattatggt taacctccat tgcctttgta tttcaggctt gctatggcac tccgcaagac
     5461 tttggcaatg atttactgct taccgggcag gttaattcta agaaaactgg tctgcctgta
     5521 aaagggataa aggtatcggt ggtggatggg atacaatata tcaaaactga tgatgccggt
     5581 aaattttccc tttatatcgg aaaaacagaa agtataagac ttaaattcga agatgttgat
     5641 gctgatgcga atggcacatt ccagactagg gatacactgc tgacaaccag taaagacgtg
     5701 gatgaaataa atctcgctat ccagctggaa gaaaaataga tgaacgcctc acttcaacaa
     5761 tttattttca ggcagttcag aaaatccgaa actaaacgtc acgaactcag ttatttgttt
     5821 tgggaatgca ctacccggtg taatctcaat tgccggcatt gtggcagcga ttgctccaaa
     5881 gacagcctat tcaaggatat gcccctgacc gactttttga aagcgctgga taccatccct
     5941 tcaccctccg aaaatttcac agtcgttctt acaggcggcg aaccgctttt gcgcactgat
     6001 attgaacact gcggaagaga aatccgaaaa cggggaatgc ggtggagcat ggtttctaac
     6061 ggttatttgt acgacgaagc gaaacacatc gcactgctta atgccggcct gggagcgctc
     6121 accatcagtg tggacgggct ggaacaatca cacaactggc tccggaataa caataaaagt
     6181 ttcgaaaggg ttgacagggc tataggcctg gcagccggca ctccgcgcct caactttgat
     6241 gtggtaacct gcgtaaatca gcgcaacttt catgagctgg aaaaaattag aaaatacctg
     6301 ataagcaaag gtgtgaaagc ctggcggctg tttaccatta ttcctattgg ccgcgccaaa
     6361 gacgatgcgg aaatgtttct gaccgataat cagatgaaag aactgatgga attcatcagc
     6421 cggtcaagaa aaaaagagga tatcgaagtc aaattcagct gcgaaggcta tgtgggcaac
     6481 tatgaaccaa aagtgagaga tacgcccttt ttctgtcgtg ccggtatcaa tatcggttcc
     6541 atactgattg atggttccat ttcggcctgc ccaaacatcg accgaacgct ggctcagggt
     6601 aatatctatc gcgacaattt ctttacagtc tggcagacaa agtatcagtt gtttcgcaac
     6661 cgatcttgga cccgaacggg caaatgtgcc gattgcaagg attacaggga ctgccggggt
     6721 aacggacttc actactggca cggcgacagg acgaatccgc tggtctgcca caatgaacaa
     6781 atcaaaaacg caacgattta aggtcaatta ttccttaaaa gttcaaatta cccgactggt
     6841 tgagaatatt aattcgtcat acatgttgca caaacaccat taaaaaagcc acctgataag
     6901 tggctttttt aattgtaaac aaacagaaaa ttactttgat agaggcattg ggtgtatata
     6961 gttaactttt tttcggatct tcctcttcgt cgtaaaagga catacttgta ttttccttcg
     7021 tatgaatttt ctgactatac ctcattctgg cattttgttc tttcacaaac atttgtttca
     7081 tatcagcctc gtttttgttg aactgcatgg tgttggtgat ggagatggaa acagcaaatt
     7141 tctctacatc gtggtcggca ccgatgtagg taaatgtcca gtttttttgt gaaagttctt
     7201 ccacaagttg tttaatagtg gcacccgaga attctttcga agaattttct tcaccatcgg
     7261 tgagaatagt aaccagcacg ttgtattcgg attttccttc cagttctttt ctaagtttgg
     7321 caaagctgaa ccccatggcg tcgtatagcg gtgtcattgc atccggttga tacttttcat
     7381 catcaatctc attgagtttt tctaccggtt cgcaaaaatg taatgttttc atgcccagtc
     7441 cattgaacgt gacgaaggta atcaggtgtt cctgctcagg gtattgcagg gcaatgcctt
     7501 tcacggtttg aactatttcg ttgaatccct gaataatggt gtttttaatt gaagtcatag
     7561 aaccgctttc atccagcaga ataagattgt gtacctggtg ttttgtttcc ataatgtatg
     7621 ggtttttaga ttattttatt gcttaaaaat accaaaccac aaagatagag cggaagtttg
     7681 acagcttatg acacaaaata tttatttttc attttaacta aattattaac aatataatcc
     7741 ctcagttcgg gtgaattgat gatttcaata tcttcgggca gacccatcac aaaccggcct
     7801 acaccttccg gagagcacac atctgtatcg agcaaccatt gattcccggg taatttgcgg
     7861 agctgtgggg ccgccagtgg aaactcctcc acgagcaggt tcatagcccg cagcccgagt
     7921 ttcagtgtga tgtggaatgt ctcactgctg ttcatccgga aaatatctat tttgccggtt
     7981 ttgtgctttc gggtgtgttt ccagtatccg ggttgaatat ccactgaacc gatgcgggaa
     8041 accttgaaaa gcttattgca gttttcatcc gggcaatagc accacacctg cacgtagttg
     8101 gtggtgaagg cgaaggcttc cacgcagcgg tcgcgtatgt cattgccatg tgccgatgaa
     8161 taattgcgca gtatcacagg gcgtctgctt tctatggcat ccaccagccg ctccacattt
     8221 cgcccgtttt tgccgttcac gatcgtttcg gccagtatgt tgtagttata gacggtgtaa
     8281 agtttctttt tgaggttttg cttgagcacg ttgttctcat cgatgctctc aattgcctct
     8341 ttgagaatgt atgcctcttc ggggctgaaa tggagcaaat cactgatgtc tttgaaatag
     8401 ggcgatgatt tgtcgagttt gatgcagttg ttttttttct tgattacaaa accggcttcg
     8461 cggaatgtgt cgatatagcg atataccgat cggggcgaga tggaaagctg gtcggctatt
     8521 tcgtccacag tcagtgaatt attggcagta agcattttca taacgcgcag caggcgctcg
     8581 agtttgggtt gatccatggc agtgaatgtg ttttttttta aggaatttat ttgtataaag
     8641 tcagaacctg acaaatatat actttttatt ctaatctgtt aacaaatctg tagcattttt
     8701 ccatttcctc tgtcaatgtc atgaattaac gcagtataac aagcctgcaa gtgacactta
     8761 acgcctgaat tagtcagtta caaaatatac gaagttatta gatcctgaaa aaatacgata
     8821 ctttcaagta ggcatcaggg acaatgaccg gaaaatttta aacaaaaaaa aggtgtccag
     8881 tcaatccgga cacctttttg atctatagtt atttattatt cacataaccg gccagcttat
     8941 tcaatcgctg cagcgttagg ttctgaaggc caatacggat tctgatacag attggatgtg
     9001 gctgcatctc cggtgggtga tgccagctgc atttgtctgt aaggcattgg tgaaagataa
     9061 tttgctttac tccagttgta accgttaaat acaagattgg ttgataaatt aacgatttga
     9121 taaggacgca aatatttact caaactttta ttggacacgt tagcttttga agaaccatca
     9181 gcgattaatc ctgtagaggt aacttcaccc ggtttaggat cggtatatac cttatcatta
     9241 taggcttcat cccaaagatt aaatccttcc actatataat tggaaacctg atccattgcg
     9301 cgccatctta ctacgtcgtt aaaacggaaa ccttcgcttg ccaattcgtt tcttctttcc
     9361 ctgcggatat tatacaaggt tgcatctacc atattaccag cagaatatgc gccccagtca
     9421 cttttttctt tatccatatc ggtagcagct atcgttttgg tatagtcggt atcaacgccg
     9481 gcgcgtgttc ttaaagcttt ccagtatttg tcagcatctc cgtctattga ttttttacgg
     9541 atgtagcaag cttcaatata gttgaggtaa gcttctgaag cgcggaacac aatgcttccc
     9601 gaaacacagg ttaagtcaga gccaggagct tcggtagggt cataattcaa gtatttacgg
     9661 cttctgtatc cggtcacgtc tcttgtttca ggaatattga ttatcgatgg tctgccgaat
     9721 aaatctgcat ctttctttac agtacgaaca tcttcttcac ctgcaaggaa aagctgaaga
     9781 cggaggtcac ggtctttttt gacatccata atcttcacat ctccctgata acctgaaccc
     9841 gctgcatacc agggtaatcc gtttttcatc agatagctgt caaccagact tttggtatat
     9901 ccggtattac caccgcgttc aacatagata gtcaccccgt gtgttacact caagccaagg
     9961 ttgtattgac gccacatggc tacttccgca aacttactca aatctttgga tccaaacatt
    10021 tcaaagtatt ggttccagcc agagaattta tctcttgcag gaggattggt aactccggag
    10081 ttagctgtca atgcagtttt atcggctact tctttggccg ctgccattgc ctgatcgagg
    10141 aagaaattaa tttccgtatc aatttcgatt ttgaaatcct tattatactc catggaagct
    10201 cccggccatc ccggtccacc cggaacacgg ggagtgcctt tgtggtaatt caggaaactg
    10261 ccttcgtaca gagctactct tgatttggca attaatgcgg cttcttttgt aattcttact
    10321 ttattgaacg aagaacttga aagaagaaga atagcagaat ctaaatcgga taaaatctga
    10381 cgggctacca ggtttcttgg agcgcgcttt gcagctgcaa taagtacatc tccctggtcg
    10441 ggtaaagtgg tttttacgat aggaaaatcg ccatacgttt taagcttctc aaaataagcc
    10501 atagcgcgta tcatgtaaat ttcgcctacg tagtgattga tgttttgtga attgccggaa
    10561 atttttccat ctttccattt tggcaatacc tgttccaaaa agtagttaca tccgcgaatg
    10621 gttccaaaac ctaaatcacc atcattgggt accagccagg tgcctttggt atataaattc
    10681 aggttgggat tagcagttgc ctgattgtca gtaccattgt cattgttgcc gatacctaca
    10741 ccccaaccac cgtgtgatgg gaataccgat ccgtaatagc tgaggggata ggaagctaag
    10801 tcagcttcag aattcaggaa tacacccggc cctacctgac taagaggtgg tctgtcgagg
    10861 aattcattac atgaattaaa tgtaatcagc actgccagca atcctgcagc tgacagcagt
    10921 tttttattta attttactag tttcatagtt tctaattatt aaaagtttaa gttaacacca
    10981 aaagatactg ttctgctcaa aggataaagt ttaccttgtc cccagtcgcc acccaacaat
    11041 tcaggatcaa aaattcctgt aatctgagat actgttaata gattatcagc ggaaacatat
    11101 attctggctg actgcattgc tgcatatttt acaatagaag ccggtaaagt gtaacctaac
    11161 tggatgtttt tcaaacgcat gtaagctgca ttttgcaaat atctggtctg tgtctgttgg
    11221 ttttttgaac tttgactaaa ctgaggtctt gggtaatatg catccagatt agctcccaac
    11281 ggatcacctt caggacggaa gaagtcccaa tgctgagtaa aagctgctga ttgccacata
    11341 ccgccactgg caccccaaaa ataagggcca cctaacatat aatcacgttt ggctacaccc
    11401 tgcaggaatg ctctgaaatc aaagccttta taagcaacat caagtgtaaa accatattta
    11461 aatcttggat ttgaattacc gataaccgat aaatcgccat gatcatccag ggttgatttt
    11521 ccactattca ctttttggtc atcgttcagg tctttataca ttacatcacc ggctgcccag
    11581 ttacttcccc acgatggctt gttggttgcc aggtgattaa tcatctcttc attggatttt
    11641 gcaatgcctg ccgtagtata tccccatatc tctccaacaa cctgaccttc gtaccagtta
    11701 ctaagcgctt ttgttgggtt cgggaaacgt agaatttgct gtctgtcatc cgataaaacg
    11761 aatttaactc catagcttaa atcactgatt ctgtcacgcc agttcagttc cagttcccaa
    11821 ccctttgact ccatgtcagc gttgttcaac ttaggaactg aagtgcctaa tattgacgac
    11881 aactcaatgg cctgaccaat catattcaat gttctgcggt taaataactc aaaagagccg
    11941 gtaaaacgac cattcaatgc attccagtcc aacccgatat tagatgactg gatggtttcc
    12001 caggtcatca gtgagcttac gatgccgggt gctgaagatg tatttggttt tacatttccc
    12061 acgagccagg tacctgaacc ggtagatatt ggcatactca ggaaccaggg atgccactgt
    12121 gtggtattca tattgcccag ttgtccccat gaagctctca gtttaagagt tgagattggt
    12181 gtattcagat ttttgaagaa gtcttcgttg gcaatattcc atccggcaga gaatgatggg
    12241 aatgtacccc acattttgtc gttgataaac cgggaagaac cgtcatgacg taaattggct
    12301 tctaacaggt atttttcttt gtaattgtag ttaagacgac caaagaatcc aactgttgac
    12361 cattggtcat atccacctga taaacttgga gtgctgtttg tagcggtatt gatagtcgga
    12421 atcaaaggag taatcaaatc tttctttgat ccacccaacg ttctggtttt catcaattca
    12481 atgttagtac ctgccagcac tttgaagttg tggtcattga tgctttttga ataatcagta
    12541 tataagttta cactccagaa attgtactta taggtgcttt caccaactgc agattgtccg
    12601 gcatcgccac cttcccaggg agcagcaaat ggctggtttt ttgcatcata accataaata
    12661 ggcaatttgt cccaatggtt gaaattgatg gtagtattat aactgccctc cagtcttaca
    12721 aaccagtctg aaagaggctc aagagataat ttcaattgtt gattcagata atctttctca
    12781 ttcttatcaa gacctccgcc ttccatttga agaatttcat ttccttccat gtagtgtccg
    12841 tttggatcat aaaccgggtt agtcggccag cgacgtgcta tgttgtggaa aaataatccg
    12901 gtcatatagg caggacgact gtaagtttcc cgaatccatc tgttgttgta gtctaatgta
    12961 gcccacggag ccatttttat ggacattttt ccgttcaatg aataacggtt gaatttatct
    13021 tctccatgac gaatcaatcc tttttggtcg agaaggtttc cgctaaccaa aaaagtaact
    13081 ttatcagtac caccgctaac attcacatta tgttcctgtg caggtgccca gtccgaatac
    13141 atttccttga accagttggt acttgcattg gcaccaccat acatcaacca gcgattattt
    13201 gaatctgctg tggttccgta catgatttga tttctgtttt catcagtcag tgttccggta
    13261 tatgttccgt tttgataagc ctgataatca agaatattct gcagcgcttt gtcggagaat
    13321 acagctccct ggccatcgtt gatagcagcc tggttaaagt attgtgcaaa ccggtatgag
    13381 tctaacatct cgggaatttg tacggcattt gaataccgga ggttaccacc gtatgatact
    13441 ctcgtttttc ctgttttacc cgatttggta gtaattaata taacaccgaa agaagctctt
    13501 gcaccataaa tggaagaaga agcggcatcc tttaacactg aaatgctttc aatgtcgttg
    13561 ggattaagcg cattcatgtt tccttccaca ccatcaatca atatcaaggg tgatgcagca
    13621 gatccatcgc caatggagcc tgtaccacgg atgttgactg acatcgaact gttcagtgca
    13681 ccaccggaat tggtttgtga taagttcaac cccggaacga taccctgcag tgcctgagat
    13741 acgttctgta ccggtctgga ctcaagttct tttgatgaaa cggttgatac tgcaccggtc
    13801 acattcactt tcttctgtgt accataccca acaactacca cttcctcaag catttcggtg
    13861 tcttcgtcca tagttacgtt gactacggat tgtcccgaaa cgttaatggt ttgcgttttc
    13921 attcccacat aactcaactc taaatttgcg ttttggggaa cattgttcaa agtgtatact
    13981 ccgttgaagt tgctgacagt gccattagta gtgccctgta ccttcactgt aacaccaatt
    14041 aacggctcat tttgcttgtc cacaacagtt cctgtcactg ttttggtctg tgcatagccc
    14101 gacaaagtaa tcgcacacat caaaatggtg agaatacttc tccaatgaat taaaattttt
    14161 tgtttgttca tgctcatttt ttaatagtta gtattattta gtattagata atcttatcat
    14221 tattctgttc gtattttgac tgaattgttt gaatatttta ttaagttcat gacttttgat
    14281 agtcagcgac ataccagaaa gtgctatttt cttcaaaatc taatattctg taattcagta
    14341 aggaaaataa aaatgctaat taaaagagaa taaaaaacga ataaaattaa aaacgaccca
    14401 aagataaata aaataattta ttaaatgaat gaataaaaaa ataatagcgc atgttatatg
    14461 cgctatttaa tcaatattaa cttgtggtaa gttaaaatta agacttaaaa cattgaaatc
    14521 cggtggatcc ggagggttag tgatgtggta aataaatgga aaaaataaaa tatgagccat
    14581 ttaattggaa agcaatctgt ttcttcctaa caggcaggcg ccataaacac cggctttgtt
    14641 tttcaatttt gaaagttcaa tttgggagtc cttgttgacc agattgaggg agtattttcg
    14701 gatagcgctg cggattggca gcagtacaaa accgcctgta tcggcaacct gtccacctat
    14761 taccaccagt tcagggttaa atatgttgat aagtccggcg atgtatttac ccagttttgc
    14821 gcttacagct tccaccactt ctatacacag ggtgtcttcc tgattggttg catcgatgat
    14881 atctgtcagt gtaaggttgt tgacgtcatc cacgcgatcg gagagtatgg atgattcgcc
    14941 ttttttaatc ctgtcgagca cggtgcggta gatggcaaat ccggaagctt cggtctcgag
    15001 gcagcctttt ttgccgcaat ggcagatcat ttcattgtcg aaagcaggaa aatggccaaa
    15061 ttcacctgaa aatcccgatt ttccgtagta tggtttacca tcgataatga tgccaatgcc
    15121 caaaccccag ctgtaattga tcaaaagtac atttttttgt ccggttacta ttcctttcat
    15181 atattcacca taagtcattg cccgggtatc gttgtcaata cctacatttc ggttgagttt
    15241 ctttgaaagt atgctgctta taggctcttc gctaaagtaa aaactgctgt agctgtaacc
    15301 cgactcgggg tttactctgc cagcaatatt caggttgata ttgaagatat tatttctgat
    15361 ttctacgggc tcttcattga taaactcctc aactttggta caaaggtcgt ccagcgattc
    15421 aagggtgttc tcatagttgt accgtatatt cattttaatc ttcactatat ccccgctaaa
    15481 atccataagt gccatattca aataatcgcg ggtcatatca atgcccatga aataggctga
    15541 agttggattc aaaccgtaaa aattcgggtg tcggccttcg ggggtgggca ttttaccgta
    15601 gtcagataaa agcccgtcat ccgacatgtt attgatcagc ttagtgacag taggaacgct
    15661 taagtccatt tctttggcaa gttcggaaat tgaagttttt ccgtaattca gaaaataact
    15721 gatgatttct ttcttgattt ggctgctttt cgtgttttgt tccagtttta aaaacttatc
    15781 ttttactgtc attgctttaa ttattatcag gttattacta atcaaagaaa ttgcaaatgt
    15841 tgaaggaata gggcaaggaa agaattagaa tacaaacata ctaattttta ttaataaata
    15901 tatgtacttt caaaaaaaat aaatatattt ttaccattgt tgtccggcca aaaaacacag
    15961 aaacgcctgt aatgtgcact aatacagata aataggaata tttagatccg tctaaacttg
    16021 cttttctgta gttgcaaaaa cgctgcaaat attttggaac gctattacta aaaaagccac
    16081 agtgtggcta aatctttgta gtatgaagtg aaaaaaaaga attttgccac agcgtggcaa
    16141 tatattagtc ctatcggata acagtgtgtt tttaaggtga atgtattaaa gctaagatat
    16201 atagataaat agctgtattt taaagttctt ttattatgat gactctttca ttattgtatg
    16261 tttgaaatca taccggaaat attccacgaa tgctattcat atgcgtttaa taaaatcaaa
    16321 caatttgtgc cgtcaactga gctttggtgg caccataaga tttttttcaa aataatattt
    16381 actatcaatg aattttctgt tggcagaaaa caagaaaaac cggctgcaaa aagccggttt
    16441 tattttttta gcgaaaatcc gaaaaatgac tgatgataat tgtttacata agagtttttc
    16501 tatcttttac caactacaag cgtgacagta ccttcgcctt tccaggtcat tatgcctgta
    16561 gtaaaatcaa ccgaacctat catat
//
LOCUS       DAOPZK010000040        16293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3781_length_16293_cov_24.637332, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000040 DAOPZK010000000
VERSION     DAOPZK010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16293
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3781_length_16293_cov_24.637332"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1298
                     /locus_tag="PKH68_03950"
     CDS             180..1298
                     /locus_tag="PKH68_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX12122.1"
                     /translation="MKKILKIALWSLLGIGVLATFYYLYQKSQPKIINYQIEQVKTGT
                     VEKRTVATGKVEPRNEILIKPQMSGIISAVYKQAGDNVTAGDVIAKIKVIPDMVNLSS
                     AESRVSRAKIAIDQSKINYDRDKELYEKKVISKEEFEKTRLLYNNDMEELKAAQNNLS
                     LVRDGISSNTSAYSNTLVRSTVTGTILDIPVKVGNSVIQSNNFNDGTTIATVANMNDM
                     LFVGKLDETEVGRVHVGMPINITIGALQDHKLPAKLEYISPKGTEENGAIMFEMKAAV
                     NVPSDLFVRAGYSANAEILLNEAKDVLTIPESCIEFSGDTAFVYLLKKPQPQEFTKKQ
                     VKIGLSDGVNIEVKEGLKIKEKIRGAEIVEKKKDKPQS"
     gene            1305..2609
                     /locus_tag="PKH68_03955"
     CDS             1305..2609
                     /locus_tag="PKH68_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX12123.1"
                     /translation="MKTKYLIITGLLLAASQLPAQRLWTLEQCIDTALTHNRNIKQQQ
                     LSYKAKEIAYAQAKANLLPNLNASAGQNFNFGRSLGADNTYISSNSATSSFGISSDLT
                     LFDGLKMKYNIEAKKADLLASGADWLKVEKDIILNVSTVFLQVLQNKELLQNAANQLD
                     LTRKNMTQRKELIRAGKLAEGEIYELQAQEAKEEFSLVQAENNLQLSKLDLSQVMDLE
                     DFKDLDIVVPVNLMENELALLSAEEVYNSAVQSRPELKAAEFRLQSSEKNIAIAKADY
                     LPSLGFGAQIGTGYYNMSRLPVNEAFGDQISNNLSTALGFRLSIPVFNRFEVKNRVKS
                     AKLEVENSKIEIEKTKIDLRKSIQQAYFNAIAAKNRWESSQKSVRANEEAFRFASQKF
                     ESGRANQYEVNIAKNNLAQSISEQTQAKYEYVFRMKLLELMK"
     gene            2676..>2854
                     /locus_tag="PKH68_03960"
     CDS             2676..>2854
                     /locus_tag="PKH68_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNX12124.1"
                     /translation="MNAENVKSQMRKGYLEYCILLILKKKPAYASDIISELKEVKLIV
                     VEGTLYPLLTRLKNS"
     assembly_gap    2855..2954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2955..4454
                     /locus_tag="PKH68_03965"
     CDS             <2955..4454
                     /locus_tag="PKH68_03965"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNX12125.1"
                     /translation="NVITVEDVGEIIEIMGKPSQFVEEEADEENPQPEPKKPAGSSKK
                     FYRDTEHQLLGGVGAGLAAYFNMDITLVRVILIILLILTSGYLIPVYLLAWLIAPKAE
                     TTAQKLEMRGEDVNIETIKNKMNDAKTYLESNEFKESATSVGQRFFDIFKAFFKVIFA
                     IIGSLLAIVGVFVAAALIFGLIVLLFEPDAITNIYPELFTGLGGVTPEKIILMVIGLL
                     LVIGCPVFALISWGIKATSKEQPKSYTAIWVAVVLWLAGIFMFLSVGTDTVKKLKDSN
                     LLSFNRNGTYVIEDNPDFISESRNVATFNAIEASGAVQIELSQQKEQTISVNSLKEYI
                     PNIKTEVVDGVLKIYSIDNLIKPRIKIRVSMDSLTSINARGASNIDFATPFSVKRLNI
                     TLQGASKADMDFSSAQNLKFDMEGASKIELKGYADTLKIDAAGATKVEAEDLQTKVAD
                     IEMNGASKATVFATESFSGKAHGASKIVCKGNPKQKNNESNTASKIIYE"
     gene            4474..5253
                     /locus_tag="PKH68_03970"
     CDS             4474..5253
                     /locus_tag="PKH68_03970"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNX12126.1"
                     /translation="MKKIFLMLSLVIAFGFQSFGQKNNAKEDKESGEYEIITENRNVP
                     QFENLNVTGRFRVVLHQTSSQTVSVVAPDKYLEYVETTVDHNALIIKMKEPKKGEDKN
                     IFERFKTKYNDYLIRQPIEIHIGVNNLKQIVLDGASSVETESAFEGKELYINLSGASK
                     ASLEYTLSDVSVVLSGASKLEMKGRAENFQLEGSGAALCETTNLKTKNVTANLSGASK
                     AEVFATDNFDAKLSGASTMICEGNPKEVRQTSSRASKIVIK"
     gene            complement(5351..8200)
                     /gene="gcvP"
                     /locus_tag="PKH68_03975"
     CDS             complement(5351..8200)
                     /gene="gcvP"
                     /locus_tag="PKH68_03975"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNX12127.1"
                     /translation="MTDFLTNRHIGISESDEEKMLQTIGVKSLDELVEQTIPSNIRLT
                     EKIELPEPLTERQYSEHIAELGAKNKIFTSYIGQGWYDTCTPAVIQRNVFENPSWYTS
                     YTPYQAEISQGRLEALLNFQTAVVDFTGLPLANASLLDEATAAAEAATMAYNLRSRDQ
                     VKAGANILFVDENVFPQTLAVIRTRSFVQGIELQVGDYNAVKFTDKYFGAIIQYPNSN
                     GSIEDYTDFVADAHAHNCIVAVAADILSLALLTPPGEWGADIVFGSTQRWGIPMYYGG
                     PSAAYFATKEEYKRDIPGRIIGISKDANGKYALRMALQTREQHIKREKATSNICTAQA
                     LLASMAGFYTVYHGGEGIRQISQQIHSIATYISEMLGVYGYTQENENFFDTLKISLPE
                     NVEIESIKSLALEYEVNFNYFETGEIGLSIDETTDLNDVNTLIEIFATAAGNTPVYAE
                     LEDVTGLRSFDESLERKSDYLTSDVFNLYHTETDMMRYIKKLERRDISLTHSMISLGS
                     CTMKLNAATEMFPLSRPEFAGIHPFAPLDQTEGYNILIANLEDYLCKITGFDACSLQP
                     NSGAAGEFTGLLTMKSYFLSKGEASRNIVLIPSSAHGTNPASAHQCGFEIIVVKCDEK
                     GNVDIADWNEKATANAEKLAGCMITYPSTHGIFEATIKDMCATIHKNGGLVYMDGANM
                     NAQVGLTNPGAIGADVCHLNLHKTFAIPHGGGGPGVGPICTAKHLSEFLPDKKFGKNT
                     VASAPYGSAGVLPITYGYIRMLGEAGLERATKIAILNANYLAARLKDSYGVLYTGETG
                     RVGHELILECRNFKSTSGVTETDIAKRLMDYGYHAPTLSFPVHGTLMIEPTESESLAE
                     LDKFTDAMLKIYGEIKEIEDGTADKTDNVLMNSPHPEYEIVGEKWEHPYTRAKAAYPA
                     DWVAENKFWINVARVDNAFGDRNLVCTCRPTSDWE"
     gene            complement(8386..11112)
                     /gene="ppdK"
                     /locus_tag="PKH68_03980"
     CDS             complement(8386..11112)
                     /gene="ppdK"
                     /locus_tag="PKH68_03980"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNX12128.1"
                     /translation="MSNTKRVYTFGNGKAEGKAEMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTDVCNEYFEKGKDNVVAELKPEVEAAMAGVEELMKSKFGDVENPLLVSVRSGA
                     RASMPGMMDTILNLGLNDTVVEGLIRKTGNARFAWDSYRRFVQMYGDVVLGMKPVNKN
                     DIDPFEKIIEEVKEAKGVHLDTELGVEDLKDLVKKFKAAVLKQTGHEFPESAYEQLWG
                     AVCSVFDSWMNDRAILYRKMEGIPAEWGTAVNVQAMVFGNMGETSATGVCFSRDAATG
                     EDQFIGEYLINAQGEDVVAGIRTPQQITKEGSMRWAKLAGVTEEDRVANYPSMEEAMP
                     EIYKELDTLQTKLENHYKDMQDMEFTVQEGKLWFLQTRNGKRTGAAMVKIAMDMFHQG
                     MIDEKTAIMRVDPLKLDELLHPIFDKTALKNAKVLTKGLAASPGAATGRIVFHADDAA
                     EWAKEGEKVIMVRTETSPEDLAGMAVAQGILTARGGMTSHAAVVARGMGKCCVSGAGA
                     IKVDYSNKTAEVGDIILKEGDYISLNGSTGDVYLGQVATSEAELDADFAEIMMLSDKY
                     TRMNVRTNADTPADALVARKFGATGIGLCRTEHMFFEGEKIKAMREMILSENAEGRRQ
                     ALAKILPYQQEDFEGIFTAMEGCPVTVRLLDPPLHEFVPHDEKGQQEMADTMNVSLKK
                     IKQRVEDLHEQNPMLGHRGCRLGNTFPEITEMQTRAILGAALSLKKKGIKAIPEIMVP
                     LTGILSEFLVQEKIIRDTAAQLFAETGDSIEFKVGTMIEIPRAALTANRIASAAEFFS
                     FGTNDLTQMTFGYSRDDIASFLPIYLEKKILKFDPFQVLDQNGVGQLIRMATEKGRSV
                     RPDLKVGICGEHGGEPSSVEFCHTVGLNYVSCSPFRVPIARLAAAQAAIKNS"
     gene            11382..12245
                     /gene="panC"
                     /locus_tag="PKH68_03985"
     CDS             11382..12245
                     /gene="panC"
                     /locus_tag="PKH68_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNX12129.1"
                     /translation="MRILKTINELISLINELKANNKTIGFVPTMGALHEGHLTLVRQC
                     VAENEVCIVSVFVNPTQFNNKADLEKYPRNVEKDAQLLEKAGCAYVFAPSADEMYSAD
                     ELENTFEFDFGGMDRLMEGKFRPGHFNGVVQVVSKLFKLVQPDKAYFGKKDFQQLAII
                     HRMVRLMHFQVEIIDCPIVREASGLAMSSRNERLSAAERDTAANIYRILNESRNLTDK
                     MSPGELTQWVTDEVNNAGGLSVEYFDIADCETLITIDRWQPESIGCIAVFCGEVRLID
                     NIEYNVQTREN"
     gene            12252..12605
                     /locus_tag="PKH68_03990"
     CDS             12252..12605
                     /locus_tag="PKH68_03990"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNX12130.1"
                     /translation="MIVTILKSKIHRVSVTQANLNYIGSITIDEDLMDAANIIANEKV
                     SIVNNNNGERFETYVITGERGSGTICLNGAAARLVQPGDVIIIMAFAMMEMEEARKYK
                     PAVIFPDTASNKLIH"
     gene            12637..13224
                     /gene="hisH"
                     /locus_tag="PKH68_03995"
     CDS             12637..13224
                     /gene="hisH"
                     /locus_tag="PKH68_03995"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNX12131.1"
                     /translation="MNVAILKYNAGNIRSVLFALERLGVDATVTDNTDELLSADKVIF
                     PGVGEASTAMQYLQEKNLDKLITQLKQPVLGICLGMQLMCSFSEENNTRCLGIFDQHV
                     RKFAAPGFKVPQIGWNTIYDLSSPLFEGISEKEYMYFVHGYYVEKCPQSIAIADYSLE
                     YSAAIHKDNFYAVQFHPEKSGDAGQKILQNFLNLI"
     gene            13239..14369
                     /gene="tgt"
                     /locus_tag="PKH68_04000"
     CDS             13239..14369
                     /gene="tgt"
                     /locus_tag="PKH68_04000"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNX12132.1"
                     /translation="MNFELQHKDPNSNARTGLITTDHGQIETPIFMPVGTVGSVKAVH
                     FHELKEDINAQIILGNTYHLFLRPSIGILEQAGGLHKFNGWDKPILTDSGGFQVFSLS
                     ATRKMKEEGVTFHSHIDGSKHLFTPERVVDIQRIIGADIIMALDECTPGTADYTYAKK
                     SMELTHRWLERGVKQFDTTEPKYGYSQTFFPIVQGCVYPDLRKQSAEFIASQNREGYA
                     IGGLAVGEPTEVMYEMIEVVNEILPADKPRYLMGVGTPINILEAIERGVDMFDCVMPT
                     RNGRNGMIFTSRGIMNMKNEKWKNDFSPLDEFGSSYVDRFYSKAYVRHLFVSKELLAM
                     QIASIHNLAFYLWLVGEARTHIQQGDFSSWKKEMVTQLGKRL"
     gene            14407..15501
                     /locus_tag="PKH68_04005"
     CDS             14407..15501
                     /locus_tag="PKH68_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNX12133.1"
                     /translation="MNYQKIGLKKIDTYIIKKFLGTFFYSIILILSIAVIFDLVEKID
                     DFYDNNAPFKAIVLDYYLSFIPYYANLFTPLFTFISVIFFTSKIANNTEIVAILSSGV
                     SFIRLMRPYIISALVITLFSFLLGAIVIPPATAKLLDFEDKYIEKFKSDNARNVQMEV
                     SKDVIMYIERYEISSNTGYRFSLEKFDKKTLVSRLTGESVTWKSKNNWTVNNYVKRDF
                     NGMRETLTRGAVLDTVIQVQPKEFFITSKEAPQMSLVDLRNYLVTQKKRGIGNIQKFE
                     DEYFRRFSMPISVLIMTLIGVSLSSRKVRGGMGLNLGIGLGLSALYVLFISMSTSFAV
                     SGTMPTVLAVWLPNIVFLTVGLFLYKTAPK"
     gene            complement(15610..16242)
                     /locus_tag="PKH68_04010"
     CDS             complement(15610..16242)
                     /locus_tag="PKH68_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNX12134.1"
                     /translation="MKLVVFGASGKTGSLLIEQALASGYEVNAYVRTQESIKLVHPDL
                     KVFVGQLSEKSKLRLAITGADACISTLGGASLTKHSPQIIQGIDNIVSIMEEEKVKRI
                     LYLSSIGAGNSRNYMPQPIRFFIADLMLRVPLADHTANENRITQSQLEWTIIRPGGLT
                     DGAKSDNLKHGTENMKMKGNQRISRSNVAAFILNQLTNSNYVNKSVWLFE"
BASE COUNT         4751 a         3533 c         3517 g         4392 t
ORIGIN      
        1 tttttcaaag atccgcaagt cagttttaca gtggctatcg gtgccctgtt catactggtc
       61 attatcggac tttttgcagg ttatctgcca gctcgtagag ccatgcaaat caaagccatt
      121 gaggctatca gagacgaaaa ttaatttatt caacataatt tttccaaaca aattcttata
      181 tgaaaaaaat tttaaaaata gcgctttggt cgctgttagg tatcggagta ctggctacat
      241 tttattattt gtaccaaaaa tcacaaccca aaattatcaa ctatcagatt gaacaggtaa
      301 aaacaggtac ggtggaaaaa agaaccgtgg ctaccggtaa agtggaaccc cgcaacgaaa
      361 ttctgatcaa accacagatg tcgggtatta tttcggccgt atacaaacag gcaggcgaca
      421 atgtaactgc cggcgatgtg attgccaaaa tcaaggtgat accggatatg gtcaacctca
      481 gttcggccga atcacgcgta tcgcgtgcaa aaattgctat tgatcagtcg aaaatcaatt
      541 atgaccgcga caaggaattg tatgagaaaa aagtgatttc gaaagaagaa tttgaaaaaa
      601 cccgacttct ctacaacaac gatatggagg aactgaaagc ggctcagaat aatctcagcc
      661 tcgtacgcga cggtatttcg agcaacactt cggcctacag caataccttg gtgcgttcca
      721 ccgtgaccgg caccatactg gatattccgg taaaagtggg taattccgtt attcagtcca
      781 ataatttcaa cgacggaacc accatagcca ccgttgccaa tatgaacgat atgctttttg
      841 tcggcaaact ggacgaaacc gaagtgggtc gtgtacatgt gggaatgcct ataaacatta
      901 ccattggtgc actgcaggat cacaaactgc ctgctaaact ggaatatatc tccccaaaag
      961 gaaccgaaga aaacggagcc atcatgtttg agatgaaagc tgctgtgaat gttccatccg
     1021 acctgtttgt tcgtgccgga tacagtgcca atgccgaaat cttgctcaat gaagcaaaag
     1081 atgtgctcac catacccgag agctgcatcg aattcagtgg cgacactgct tttgtatatc
     1141 tgctgaaaaa accacaaccc caggaattca caaaaaaaca ggtgaaaatt ggcttatcgg
     1201 acggagtaaa tatagaagta aaagaaggcc tgaaaatcaa agagaaaata cgtggtgccg
     1261 agattgttga aaagaaaaaa gataaaccac aatcctaact tagaatgaaa acaaaatatt
     1321 taattatcac gggattactg ctggcggcct cacaacttcc agctcagagg ctttggaccc
     1381 tggaacaatg catcgatacc gcattgactc acaaccgaaa tattaaacag cagcaactga
     1441 gctacaaggc aaaggaaatt gcttacgcac aagcaaaggc aaatttactc cccaacctga
     1501 atgcgtctgc tggtcagaat ttcaactttg ggcgttcact cggtgccgac aacacctata
     1561 tcagttccaa ttcggctacc tcatcgtttg gaatttcgtc cgatctgacc ttgttcgacg
     1621 ggctgaaaat gaaatataac atagaagcaa agaaagcgga cttgctggca tcaggagccg
     1681 actggttaaa agttgaaaaa gatattattc tcaatgtatc aaccgttttt cttcaggtgc
     1741 tgcagaacaa ggaattgttg caaaacgctg caaatcagct tgacctcacc cgaaagaaca
     1801 tgactcagcg aaaagaactg atccgggcag ggaaactggc tgaaggtgaa atctacgaac
     1861 tgcaggctca ggaagcaaag gaagaatttt cgctcgttca ggccgaaaat aatcttcaac
     1921 tgtctaaact ggatttgtcc caggtaatgg atctggagga ctttaaggac ctggacattg
     1981 ttgtgccggt caatctgatg gagaatgaac tcgccctgct atcggccgag gaagtttaca
     2041 acagcgccgt gcagtcgcgt cccgaattga aagctgctga attccgcctt caaagcagtg
     2101 agaaaaacat tgccattgcc aaagccgatt atctgccctc gctgggtttt ggagcacaaa
     2161 tcggaaccgg gtactacaat atgagtcggt tgcctgtgaa tgaagcattt ggcgatcaga
     2221 tatccaacaa cctgagtact gcgttgggtt ttcgtctttc gataccggtt ttcaatcgct
     2281 ttgaagtcaa aaaccgggta aaaagtgcca aactggaggt ggaaaactca aaaattgaaa
     2341 tagagaaaac aaaaattgat cttcgtaaat ccattcaaca agcttatttc aatgccattg
     2401 ctgccaaaaa ccgttgggaa tccagccaaa aatcagtgcg agccaacgaa gaagcctttc
     2461 gatttgccag tcagaaattc gaatccggca gagccaatca gtacgaggtt aatattgcga
     2521 aaaacaatct ggcacaatcc atatccgaac aaacacaggc caaatatgaa tatgtgttcc
     2581 ggatgaaact gctggagctg atgaaataaa cacctgattt acaatcagta aaagacatct
     2641 aacgactaat ttaaattttt gtttaacaag aaactatgaa tgcagaaaat gtaaaatcac
     2701 aaatgcgaaa agggtatctt gaatattgca tactgcttat tctgaaaaag aaacctgctt
     2761 acgcctcgga tataatttcc gagctgaagg aagtgaagct gatagtggtg gaagggacgt
     2821 tgtatccact tctcacccga ctaaaaaaca gcgannnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnaatgtg attaccgttg aagacgttgg cgaaataatt gaaataatgg
     3001 gaaaacccag ccagtttgtg gaggaagaag cggatgaaga aaatccgcaa ccggagccta
     3061 aaaagccggc cggaagttcc aaaaaattct atcgggatac cgaacatcaa ctccttggag
     3121 gcgtgggagc cggacttgct gcttatttca atatggatat tacgctggtg cgcgtgatat
     3181 taatcatcct gcttatactt acatcgggtt acctcattcc cgtgtatctg ctggcgtggc
     3241 tgatagcgcc caaagccgaa actacagctc agaagctcga aatgcggggt gaagatgtaa
     3301 atatcgaaac cataaagaat aaaatgaacg atgcaaaaac ctatctggaa agcaatgaat
     3361 tcaaagaatc ggccacttct gtgggacaac ggtttttcga tatattcaaa gcctttttca
     3421 aagtaatttt tgccattatt ggatcgctgc tggctattgt aggtgtattt gtggccgctg
     3481 cacttatttt cggattaatc gtgctgctat tcgaacccga tgcaatcacg aacatttacc
     3541 ccgaactttt cacaggtctg ggtggcgtta ctcccgaaaa aattatcctg atggtcattg
     3601 gattattgct cgtaattggc tgtccggtat ttgcactcat cagctgggga ataaaggcaa
     3661 cctccaaaga gcagcctaaa tcttacacag ccatctgggt agcggttgta ttgtggctgg
     3721 caggcatatt catgttcctg agcgtaggca ccgatactgt taaaaagtta aaagacagca
     3781 atttgttgtc attcaaccgg aacggtacgt atgttattga agataatcct gattttattt
     3841 ccgaatcaag aaacgttgcc actttcaatg ccattgaagc ctcaggggcc gtacaaattg
     3901 aacttagcca gcaaaaagaa cagaccatat ctgttaactc gctgaaggag tacattccga
     3961 atattaaaac tgaagtagtt gacggtgtac taaaaattta ttccatcgat aatttaataa
     4021 aaccacgcat taaaatacgt gtaagcatgg actcacttac ctcaatcaat gccagaggtg
     4081 cttcaaatat agattttgca actccttttt cggtaaaaag actgaatatc acattacagg
     4141 gagccagtaa agccgatatg gattttagtt cggcacagaa tttgaagttc gacatggaag
     4201 gagccagtaa aatagaattg aagggatatg ccgacacgct gaaaatagat gctgccggtg
     4261 caacgaaggt tgaggccgaa gacttacaaa ccaaagtggc cgatattgaa atgaacggtg
     4321 ccagcaaagc aaccgtattt gcgaccgaat cattcagcgg aaaagcccac ggtgccagca
     4381 aaatagtatg caaaggcaat ccgaaacaaa aaaataatga atcaaacaca gcttcgaaaa
     4441 tcatttatga ataaaaacta aaaagtaagc atcatgaaaa agatcttttt aatgcttagt
     4501 ctggtaatcg cttttggatt tcaaagtttt ggtcaaaaaa acaatgccaa agaggataag
     4561 gaatcgggtg aatacgaaat tattaccgaa aacagaaatg tacctcaatt tgaaaatctg
     4621 aatgtcaccg gccgattcag ggtggtactg caccagactt catcgcagac tgtatcggtc
     4681 gttgcacccg acaaatatct tgaatatgtg gaaacgacag tggatcacaa tgcactcatt
     4741 atcaaaatga aagagccaaa aaaaggtgag gataaaaaca tttttgaaag atttaagact
     4801 aagtataatg actacctgat ccgtcagccc atcgaaattc acatcggtgt gaataacctg
     4861 aagcaaatag tactcgacgg tgcttcgagt gtcgaaaccg aatcggcttt tgaaggcaaa
     4921 gaattataca tcaatttgtc aggtgcttcc aaagccagtc tggaatacac cctgagcgat
     4981 gtgtcagttg tattgagcgg agcttcaaaa ctggaaatga aaggtcgggc tgaaaatttt
     5041 caactggaag gaagtggtgc tgcactctgc gagaccacca atctgaagac gaaaaatgta
     5101 accgccaacc tgagcggcgc cagtaaagca gaggtttttg ctaccgataa ttttgatgcc
     5161 aaactttccg gagcaagcac catgatctgc gaggggaacc cgaaagaggt tcgtcaaacc
     5221 tcgtcaaggg cttcgaaaat tgttattaaa taatatttcc ccgtttttgt ttgtaatttg
     5281 ccaaaatgaa gaaacgccgt tcagtgatta gctgacggcg tttccttttt attgaactga
     5341 tttccggttt ttactcccag tcggaagtcg gacgacaggt gcacactaaa ttccggtcgc
     5401 caaaggcatt atcaacacgt gccacattga tccagaactt attttcggcc acccaatctg
     5461 cgggataagc cgcttttgca cgtgtgtatg gatgttccca tttttcacct acaatttcat
     5521 attcaggatg aggtgaattc atcaatacat tgtcggtttt gtcagcagta ccgtcttcaa
     5581 tttccttgat ttcaccataa attttgagca tggcatcggt gaatttgtcc agttcggcca
     5641 gcgattcact ttcggtgggc tctatcatca atgtgccatg aaccgggaac gacagggttg
     5701 gtgcgtgata tccgtaatcc atcaatcgtt tggcaatatc ggtttcggtg acacccgaag
     5761 tgcttttgaa attgcggcat tccagtatta actcgtgccc tacccgacct gtttcaccgg
     5821 tataaagcac accataacta tctttgagac gtgctgccag ataattggca ttgagaatgg
     5881 caatttttgt tgctctttcc agtcccgctt cacccagcat acgaatatag ccgtaagtta
     5941 tcgggagtac tcctgcactg ccataaggtg ccgatgcaac ggtattttta ccgaatttct
     6001 tgtcgggtaa aaattccgaa agatgtttag ctgtacagat aggacctaca ccgggaccac
     6061 cgccgccgtg aggaatggca aatgttttgt gtaaattgag gtgacaaaca tcagcgccga
     6121 ttgctccggg attggtcaat ccaacctgag cattcatatt ggcgccatcc atgtaaacaa
     6181 gtcctccgtt tttgtgaatt gtggcacaca tatctttgat ggttgcttcg aaaattccat
     6241 gggtggaagg ataggtaatc atacaaccgg caagtttttc agcatttgcg gttgcttttt
     6301 cattccagtc ggcaatatcc acgtttcctt tttcatcaca tttcaccaca ataatttcaa
     6361 atccgcactg atgagcactg gcgggattgg tgccgtgcgc cgatgaaggg attaaaacga
     6421 tattacggct tgcctctcct ttggaaagga aataggattt catagtcagc agaccggtaa
     6481 attctccggc agcacctgaa ttgggctgca gactgcaagc gtcaaaaccg gttattttgc
     6541 acagataatc ttccagattg gcaatcagga tattatatcc ttcggtctga tccaggggtg
     6601 caaaaggatg aattccagca aattcgggac ggcttaacgg aaacatttcg gtggcagcat
     6661 tgagtttcat ggtgcatgaa ccgagcgaaa tcatggagtg agtaagcgaa atatcgcgtc
     6721 gttcgagctt cttaatataa cgcatcatgt ctgtttcagt atgatacaga ttaaatacgt
     6781 cgctagtcag atagtccgat ttgcgttcca gactttcgtc gaatgatctc agtccggtga
     6841 catcttccaa ttctgcatag acaggcgtgt tgccggctgc tgttgcaaaa atttcaatca
     6901 gtgtatttac atcgttcagg tcagtggttt catctatgct gagacctatt tcacccgttt
     6961 caaaatagtt gaaatttact tcatattcca atgccaaaga cttaatactt tcaatttcaa
     7021 cattttcagg taacgaaatt ttcagcgtat caaagaaatt ttcattttcc tgtgtatatc
     7081 cgtaaacgcc gagcatttca ctgatatacg tggcaatgga atgaatttgt tgtgaaattt
     7141 ggcgtattcc ttcgcctccg tgatacacgg tataaaatcc ggccatcgaa gccagtaaag
     7201 cctgagcggt gcagatattc gaagtagctt tttcacgttt gatgtgctgt tcgcgggttt
     7261 gtaaagccat ccgcagtgca tattttccgt tggcgtcttt actaatacca attatgcgtc
     7321 ccggaatatc gcgtttatat tcttctttgg tagcaaaata agctgctgaa ggaccaccat
     7381 agtacatcgg aattccccag cgttgcgttg aaccaaaaac aatatcggct ccccattcgc
     7441 ccggaggagt taggagtgcg aggctgagaa tatcggcagc tacggctaca atgcaattgt
     7501 gagcatgagc atcagccaca aaatcagtat aatcttcaat gcttccgttc gagttagggt
     7561 actgaataat tgcaccaaaa tatttgtcgg taaattttac tgcgttgtaa tcacccacct
     7621 gtaattcaat tccctgtaca aatgaacggg tacggataac tgcaagtgtc tgtggaaata
     7681 cattttcatc cacaaaaagg atattcgctc cggctttcac ctggtcgcgg ctgcgcagat
     7741 tgtaggccat ggttgcagct tcggcggcag cggtggcttc gtcaaggaga gatgcattcg
     7801 ccaggggcag acctgtaaaa tcgacaactg ctgtctgaaa gttgagcagg gcttccagtc
     7861 gtccctgcga aatttcagcc tgataaggtg tatatgaagt gtaccaggaa ggattctcga
     7921 aaacattacg ctggattaca gccggtgtac aggtgtcgta ccatccctgg ccaatgtagg
     7981 acgtgaaaat tttgttttta gcgcccagtt cggctatatg ctccgaatac tggcgttctg
     8041 tcagcggctc cggaagttca atcttttcgg tcaaccggat gttggaaggt atagtttgtt
     8101 ctaccagctc atcgagcgat tttactccga ttgtctgaag cattttttct tcatcacttt
     8161 cggaaatacc tatgtgtctg ttagttaaaa aatcggtcat agtacttttt tgattttaga
     8221 attaattttc aaaggtaata tttttaattg taaacatgga atatcattcg tgtaaaataa
     8281 aattttcttt cttctgaaaa aaaccggata ttatttttcc ttaatcaaag aagccaaatc
     8341 tgcgtgcaga tttggcttct cctataaata ctatcttttc ctttcttagc tattcttaat
     8401 agcagcctga gcagcagcca gacgggcaat gggcacacgg aacggcgagc agctcacata
     8461 gttcaatcct acggtgtggc agaattcaac cgatgatggt tcgccaccat gttctccgca
     8521 aatacctact ttgaggtcgg gacgtacgga gcggcctttt tcggtagcca tgcgtatcaa
     8581 ctgaccaaca ccattctgat ccaatacctg gaacgggtca aatttcagaa ttttcttttc
     8641 cagataaata ggtaagaagg aggcaatgtc atcgcgtgaa taaccgaagg tcatctgcgt
     8701 aaggtcattg gttccgaatg agaagaattc ggcagcagaa gcaattctgt tggcagtaag
     8761 agctgcacgt ggaatttcaa tcatggttcc aaccttgaat tcgatgctgt caccggtttc
     8821 tgcaaacaat tgtgcagccg tatcacgaat tattttttcc tgtaccagga attcagaaag
     8881 aatacctgtg agcggaacca taatttcagg aatagccttg ataccttttt tcttcaaact
     8941 caacgctgca cccagaatag cgcgggtttg catttcggtg atttcgggga aagtatttcc
     9001 aaggcggcat ccgcggtgtc caagcatcgg attttgttcg tggagatctt ctacacgctg
     9061 tttgattttt ttcaatgaaa cattcattgt atctgccatt tcctgttgtc ctttttcatc
     9121 gtgaggcaca aattcgtgca acggtggatc gagcagacga acagtaaccg ggcatccttc
     9181 catagcggtg aaaatacctt cgaagtcttc ctgttgataa ggcagaattt ttgccaatgc
     9241 ctggcgacga ccttcagcgt tttcagaaag gatcatttcg cgcatagcct taattttttc
     9301 accttcgaag aacatgtgtt ctgtgcggca cagtccgata ccggtagcac caaacttacg
     9361 ggcaaccagc gcatcggcag gtgtgtcggc attggtacgt acattcatac gggtatattt
     9421 gtcggaaagc atcatgattt ctgcaaaatc ggcatccagt tcggcttcag aagtggcaac
     9481 ctgtccgagg tacacatcac cggtcgaacc gttgagtgaa atgtaatcac cttctttcag
     9541 aatgatatcg cctacttcgg cagttttatt ggaataatcc accttgatag caccggcacc
     9601 cgatacacag cattttccca taccacgggc aaccacagct gcgtgagaag tcataccgcc
     9661 gcgggcagta agaataccct gtgcaacagc cataccggct aagtcttcgg gagaagtttc
     9721 ggtacgaacc ataattactt tttcaccttc ttttgcccat tcggcagcat catcggcatg
     9781 gaaaacaatt ctacccgtgg cagcacccgg agaagcagcc agccctttgg tcagaacttt
     9841 tgcatttttc agtgctgttt tgtcaaaaat cgggtgaagc agttcgtcca gtttgagcgg
     9901 atcaacgcgc atgatagctg ttttttcatc gatcataccc tgatggaaca tgtccatggc
     9961 tattttcacc atggcagcac cggtacgttt accgttacgt gtttgcagga accacaattt
    10021 accttcctga acggtaaatt ccatatcctg catatctttg tagtgatttt caagtttggt
    10081 ttgcagtgta tccagctcct tatagatttc gggcatagct tcttccatgg atggatagtt
    10141 tgctactctg tcctcttcgg ttacaccggc cagtttagcc cagcgcatgg aaccttcttt
    10201 ggtaatttgc tgcggagtac ggataccggc caccacatct tcgccctgag cattgatgag
    10261 gtattcaccg ataaactgat cttcgccggt agcggcatcg cgtgagaaac atacgccggt
    10321 ggcggaagtt tcgcccatat ttccgaatac catggcctgt acgttaacgg cggtacccca
    10381 ttcggcaggt attccttcca tcttgcgata aagaatagcg cgatcgttca tccatgagtc
    10441 gaacacggag catactgcgc cccaaagttg ctcgtaagct gattcgggaa attcatggcc
    10501 ggtttgtttc aatacagcag ctttgaattt ctttacaaga tccttgagat cttcaacacc
    10561 taattccgtg tcaagatgaa cccctttcgc ctctttaact tcttcaatta ttttttcgaa
    10621 gggatcgata tcgtttttat ttactggttt catacccagt accacgtcgc catacatctg
    10681 cacaaaacga cggtatgaat cccaagcaaa acgcgcattt ccggttttgc ggatgagtcc
    10741 ttcgaccact gtatcattca ggccgaggtt tagaattgtg tccatcatac ccggcatgga
    10801 agcacgtgca cctgaccgaa cggataccaa caacggattt tcaacatccc cgaatttaga
    10861 tttcattaat tcctcaacac cggccattgc tgcttctact tcgggcttaa gttctgctac
    10921 tacattatct ttcccttttt cgaagtattc gttgcaaaca tctgtagtga tggtaaatcc
    10981 gggtggtacc ggcacaccga ttaaattcat ttcggcaagg ttggcacctt ttccgcccag
    11041 cagatttttc atttctgctt taccttcggc ttttccgttg ccaaaggtgt aaactctttt
    11101 tgtgttgctc atataaattg tttttttaaa tggatatatt ctgattggtc aatataacac
    11161 ccttcaacgg gttaagttca ctgtcgtaac agtcaaacta ataatcattt aagatgattc
    11221 tgcaaagata ggatttttag ttaacatata aaaatctcaa ctcttaaata atcagacgat
    11281 gcattaaaaa attgttctaa tatcggattg ttgatggtta aaacaaattt gttttatact
    11341 tttgcagtct gaatttcaat ctgatatatt tattcctttc aatgagaata ttaaagacta
    11401 tcaatgaatt aatatcgctg atcaacgaat taaaagcgaa taataaaacc atcggttttg
    11461 ttcccactat gggtgcttta catgaaggac acctgacgtt ggtaagacag tgtgtggcag
    11521 aaaatgaggt gtgcatagta agcgtatttg tcaatccgac gcagtttaac aataaagcgg
    11581 acctggagaa atatccccga aatgtggaaa aagatgctca actgctcgaa aaagccggat
    11641 gtgcctatgt tttcgcccct tcagccgacg aaatgtattc agccgatgaa cttgaaaata
    11701 cgtttgaatt tgatttcgga ggtatggacc ggttgatgga gggtaaattc agaccgggac
    11761 actttaacgg agtagtgcag gttgtaagca aacttttcaa attagtacaa cccgacaaag
    11821 cctattttgg taaaaaagat tttcagcaac tggccatcat acaccgcatg gtgcggctga
    11881 tgcatttcca ggttgaaata atagattgcc ccattgtgcg tgaagcgagc ggactggcca
    11941 tgagcagccg caatgagcgg ttgtcggctg cagagcgtga tacagcagcg aatatataca
    12001 gaatactgaa tgaaagccgc aacttaaccg acaaaatgag ccccggagag cttacacaat
    12061 gggtaaccga tgaagtgaac aacgcaggcg gtctgagcgt tgagtatttt gacatagccg
    12121 actgtgaaac gctgataacc atcgaccggt ggcaaccgga gagcattggc tgcattgccg
    12181 ttttctgcgg cgaagtccgg ctgattgata atattgaata caatgtacaa actcgtgaaa
    12241 actaaccaac tatgatagtc accatcctga aatcaaaaat tcaccgcgtt tcggttacgc
    12301 aggcaaatct gaattacatt ggaagcatca ccattgatga agacctgatg gatgcggcca
    12361 acatcattgc gaacgaaaaa gtgagcatag tcaataataa caacggcgaa cgatttgaaa
    12421 catatgtaat caccggtgaa cggggatcgg gaacaatctg cctgaatggt gccgcagccc
    12481 gactggtgca gcccggcgac gttattatca ttatggcttt tgcaatgatg gaaatggagg
    12541 aagcccgaaa gtacaaaccg gcagtaattt tcccggatac cgcctccaac aaactgatac
    12601 actaagtttc cgatccatta attccccgct tgaaaaatga atgttgccat cctgaaatac
    12661 aacgccggaa atatccgatc agtccttttt gcgctcgagc gtctgggagt ggatgcaacg
    12721 gttaccgaca ataccgatga attactgtcg gccgacaagg tgatattccc gggagtaggc
    12781 gaagcttcca ctgccatgca atacttgcag gaaaaaaatc tggataaact gattactcaa
    12841 ctgaaacagc cggtactcgg catttgcctg ggcatgcagc tcatgtgcag tttttccgaa
    12901 gaaaataaca cccgttgtct gggaatattc gaccagcacg tgcggaaatt cgcagctccc
    12961 ggcttcaaag tacctcaaat aggctggaat actatatacg atttatcttc accgcttttc
    13021 gagggcatat ccgaaaaaga atacatgtat tttgttcacg gatattacgt ggaaaaatgc
    13081 ccgcaaagta tagcaatagc agattattca ctggaataca gtgcagccat tcataaagat
    13141 aatttttatg cggtgcagtt tcatcctgaa aaatcgggcg acgcaggaca aaaaatcctg
    13201 caaaattttt taaatttaat ctgacattag aataataaat gaatttcgaa ctacaacaca
    13261 aagacccgaa cagcaacgca cgcaccggat taataacaac cgaccacggt cagattgaaa
    13321 ctcccatttt catgcctgtg ggtactgtcg gatcggtcaa agcagttcat tttcacgaat
    13381 taaaagaaga tatcaacgcc caaatcattt tgggaaatac ctatcattta ttccttcgtc
    13441 cgagtatcgg gatacttgaa caagccggcg gactgcacaa gtttaacgga tgggacaaac
    13501 ccatattgac cgatagtggt ggttttcagg tattttcact gtccgcaacc cgcaagatga
    13561 aggaagaagg agttaccttc cactcacata tcgacgggag caagcatctg ttcacacccg
    13621 aacgggttgt tgacatacag cgcattatag gcgccgacat cattatggcg ctggatgaat
    13681 gcacacccgg aacagccgat tacacgtatg ccaaaaaatc gatggaactc acccaccgct
    13741 ggctggagcg cggagtaaaa caatttgata ctactgaacc aaagtacggt tactcacaga
    13801 catttttccc cattgtacag ggttgcgtat atcccgattt gcgcaaacag tcggccgaat
    13861 ttatcgcttc gcaaaaccgg gagggatatg ccatcggtgg tctggccgtg ggagaaccca
    13921 ccgaagtgat gtacgagatg atagaagtgg tcaatgagat attaccagcc gacaaaccgc
    13981 gatacctgat gggcgtggga actccgataa atatcctcga agccattgag cggggtgtgg
    14041 atatgtttga ctgcgtaatg cctacccgca acggacgcaa cgggatgata ttcacctcgc
    14101 gcggcatcat gaatatgaaa aatgaaaagt ggaaaaacga cttctcaccc ctcgatgaat
    14161 ttggcagttc gtacgtggat cgtttttatt ccaaagcgta tgttcgccat ttatttgtga
    14221 gtaaagaatt gctggccatg cagatagcat ccattcacaa cctggctttc tacctgtggc
    14281 tggttggcga agcacgcact cacatccaac agggagattt ttcatcgtgg aaaaaggaga
    14341 tggtaacaca actggggaaa cgactgtaac aagttgctaa acaaacagat aaagaaatca
    14401 aaaaaaatga attatcaaaa gataggtctg aaaaagattg acacctatat cattaaaaag
    14461 ttcctcggaa cgtttttcta ttccatcata ctcattttga gtattgcggt tattttcgat
    14521 ctggtggaaa aaattgatga tttctacgat aacaatgctc ctttcaaagc cattgtgctt
    14581 gattattacc tgagcttcat cccctactat gccaatcttt tcacgcccct tttcaccttt
    14641 atctccgtca tatttttcac ctcaaaaatt gccaacaata cagaaattgt agccatattg
    14701 tccagcggtg tgagctttat ccgtctcatg agaccttata tcatttccgc cctggttatc
    14761 accctgtttt catttctact gggagccatt gtcatacctc cggccacagc gaaactgctg
    14821 gattttgagg ataaatacat cgaaaaattc aaatcggata atgcccggaa tgtgcagatg
    14881 gaagtgagta aagatgttat tatgtacatc gaacgctacg aaatttcgtc gaatacgggt
    14941 taccggtttt cgctcgagaa atttgacaaa aaaacgctcg tttcccgtct caccggtgaa
    15001 tctgtcacct ggaaatcgaa aaacaactgg actgttaaca attatgtgaa acgcgatttc
    15061 aacgggatgc gtgaaaccct gaccagaggt gcggtacttg atacagtaat ccaggtacaa
    15121 cccaaagaat tttttatcac ctccaaagaa gcgccacaga tgagtttggt ggacctgcga
    15181 aactatctgg ttacacagaa aaaaagaggt ataggcaata tccagaaatt cgaggatgaa
    15241 tatttccgcc gtttctcgat gcctatttcc gtacttatca tgacattaat cggcgtatcg
    15301 ctctcgtcac gcaaagttcg gggcggtatg gggcttaatc tcggcatcgg acttggactg
    15361 agtgcattgt atgtgctctt catctccatg tccacctcct ttgctgtaag tggaacgatg
    15421 ccaactgtac tagccgtatg gctgccgaat attgtttttc tgactgtagg gttatttctc
    15481 tacaaaacag ctccgaaata aaaacatccg acaggcagat attaacagat aacaaaggga
    15541 gcgaccggac aggttgctcc ctttgctttt ttttcttaaa aaattttcaa cgtttgtaat
    15601 aacaattatt tattcaaata accaaacgct tttattcaca tagttactat tggtaagttg
    15661 attgagaata aaagcggcca catttgaacg tgaaatgcgt tggttgcctt tcattttcat
    15721 gttttccgta ccatgtttca gattatcaga cttggcgcca tcggttaagc cgccggggcg
    15781 tattatcgtc cactcgagct gactttgagt gatgcgattt tcgtttgcgg tatgatcagc
    15841 caaaggaaca cggagcatta aatccgcaat aaaaaaacga ataggttgtg gcatataatt
    15901 tcggctgtta ccggcaccga tactcgataa ataaaggatg cgcttcactt tctcttcctc
    15961 cataatactg acgatattat caataccctg aattatctgg ggactgtgtt ttgtcagaga
    16021 tgctccgccc agtgtcgaga tacatgcatc cgctcccgtg atggctaatc taagtttgct
    16081 cttctcactc agctgaccca cgaaaacttt taaatcagga tgaaccaatt ttatagattc
    16141 ctgagttcgg acataagcat tcacctcata acctgatgcc aatgcttgtt ctattaataa
    16201 ggaaccggtt ttaccgcttg ctccaaatac aactaatttc ataagtttaa attttgataa
    16261 gtttgtttat caggcaaaaa atatatttcg att
//
LOCUS       DAOPZK010000041        16104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3856_length_16104_cov_22.647268, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000041 DAOPZK010000000
VERSION     DAOPZK010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3856_length_16104_cov_22.647268"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2198
                     /gene="uvrA"
                     /locus_tag="PKH68_04015"
     CDS             <1..2198
                     /gene="uvrA"
                     /locus_tag="PKH68_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNX12135.1"
                     /translation="ATDKDRKRLKESMQKAMQHGNGIIMLLEKDKDEVRYFSRKLMCP
                     TTGISYDDPAPHNFSFNSPHGYCPRCKGLGFINEIDMAKIIPDTSVSIFNGGIAPLGK
                     HKNSTIFWQIEAILAKYECTLKTPLADVPDDAIDEIMNGTDEQLHIKNESLGNSNYFL
                     SYDGIIKYIEMQQENSASATEQKWANQYSKTIKCTECDGQRLRKESLYFRIHDKNIAA
                     LAEMDISELSEWLKNVDFFLTEKQRIIATEILKELQSRLQFMLDVGLNYLALNRSSQS
                     LSGGESQRIRLATQIGSQLVNVLYILDEPSIGLHQRDNLRLIHSLKQLRDTGNSVIVV
                     EHDKDMMLAADYVLDLGPRAGRLGGELVFAGTPTEMLQSETLTADYLTGKKQIEIPEK
                     TREGNGKFLTLKGAKGNNLKNIDVTFPLGKMICVTGVSGSGKSTLINDTLQPILSQHF
                     YRSLQDPLPYDSIDGVDNIDKIVQVDQSPIGRTPRSNPATYTGVFSDIRNLFATLPEA
                     KIRGYKPGRFSFNTAGGRCETCGGNGYKTIEMNFLPDVYVPCESCNGKRYNRETMEVR
                     FKGKSIADVLNMTINQAVEFFENQTYILNKIKTLQDVGLGYIKLGQSSTTLSGGESQR
                     VKLATELSKRDTGKTLYVLDEPTTGLHFEDIRVLLGVLNRLVNRGNTVIIIEHNMDVI
                     KSADYIIDIGPEGGNGGGRVICTGSPQEVAANTESETGKFLAQELHLHS"
     gene            2319..2525
                     /locus_tag="PKH68_04020"
     CDS             2319..2525
                     /locus_tag="PKH68_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12136.1"
                     /translation="MNQKLKLFLYGLVVFFVYAVLTWVLKLIAHKMPEGEAFLGVYTS
                     TDLWLGLVIAIILTYSHYMKMKIK"
     gene            2530..3849
                     /locus_tag="PKH68_04025"
     CDS             2530..3849
                     /locus_tag="PKH68_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNX12137.1"
                     /translation="MSEDQLSEITEYIIRFLLGSKANETFSNLIGYTSDKTLFSKYKI
                     VILPSGFFDQGFYGTAQSLPQFPLPLWDDMPLLYGQPEIAKNGDTIILHADIIVSTFF
                     LISRYEETIRRNERDEHGRFPGSKSLPFRAGFLHRPVVDEYGKQLRKLLRETGAVINE
                     PEEKFNKIYLTHDVDQLAHYRNIRGIGGAVSRFFKNPEQTFKALQSYFLGLKYDPWFT
                     FPFLIDENRKLRNELVETIFFIKTGGGNLQTDKPLHDIFDKNFQILFRLGIENGVKFG
                     LHPSYQAGIEPRLITDEKNLLDKRLNQQTSYSRNHFLASREPEDMQALIDNGFTDDYT
                     MSYADQAGFRLGTSRAVKWINPTILQLTNLTLHPLTLMDSTLSDKRYMNLIVDEAFSF
                     SKKMIDQVKKNNGDLTLLWHNTSVEKHNGLYHRDLYRWMINYLKMQA"
     gene            3882..5120
                     /locus_tag="PKH68_04030"
     CDS             3882..5120
                     /locus_tag="PKH68_04030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX12138.1"
                     /translation="MKILIIGEPFLPPAYLPRVRYFCSYFIEKGWKVDLVTENSEHLH
                     YVPENVTLLPINYYKYKQGIIAKSEWAFKFLLDLCLDYKGRYFYKKSKELLSQNHYDL
                     VFCSSSFTFPLTVAARVAEKLNRPLFVDLRDIAEQSPDDNHYIAHRPPPLLGKLITRI
                     YKNVSINRRNRVLRLATGVTTVSPWHVQTLSKHNPYTHLIYNGFDENMFVPGKLATER
                     FTISYFGRIYNEQIRNPRLLFSALSELNKKGMVTPENTVVKWFVDEQSRNVILNIANN
                     YNLSGFMEFHNFITPNALSDEMNKSSVLLVLCNAKDTKNYFGIMTTKFFEALGTNRPV
                     VCIPDNNDNLSHLIKETKCGLVSSDELEVEKFLIEKLEEWQQKKRTNGMLDETIRMNF
                     SRKKGAETLEKLFITAIKQQ"
     gene            5120..6004
                     /locus_tag="PKH68_04035"
     CDS             5120..6004
                     /locus_tag="PKH68_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX12139.1"
                     /translation="MNIKKLVPRPLIMMRREMLPGWRKITKKNMQQKKMIPILHLHLA
                     DHCNLDCRGCDNFSPLAPEVFADVDVFEKDCAKMADLSQGRIEEIQLLGGEPLLHPQI
                     NTFMEIVRRYFPTNKISIVSNGILLQTKKDVFWEACRRYKIEIIVTKYPIQLDFEKIK
                     QKVLAQNVPFSFYGNTETVEKTMECLPLDLEGKQDPKDSFLRCARANRCVSLDNGKIY
                     TCSLIPYVKYFNDHFQQNLTVTTADYLELHQATSYDEILNFVCKPMPFCRYCNIKGTI
                     WDIGYGISNKDISEWTGK"
     gene            6212..7108
                     /locus_tag="PKH68_04040"
     CDS             6212..7108
                     /locus_tag="PKH68_04040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX12140.1"
                     /translation="MLSILIPTYNYDVTRLVTELHRQALNTYVDFEILVMEDGSTQFL
                     EENKAISNLQFCHYTALSHNIGRSAIRNRLADEARYGHLLFIDCDAEVHTSHFVEKYL
                     AFCREEVVVIGGTAYEPENNDPDFSLRLKYGRMREAKSAQNREQAGKYSHFSSFNFLI
                     SKSIFNQIRFDESIRGYGHEDTLFGHQIAELHCEVHHIDNDLIHKGLDDNILFIHKTE
                     ESTKNLYLLHKSNKYPFLANQSKLLRTFINLDRKNLSPLLAKLFDVLQTCMQKQLCSS
                     RPSLKIYDLYKLLFLAKISLSD"
     gene            7263..7532
                     /locus_tag="PKH68_04045"
     CDS             7263..7532
                     /locus_tag="PKH68_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNX12141.1"
                     /translation="MNIKPLADRVLIKPAPAEEKTVSGIIIPDSAKEKPLKGEVLAVG
                     NGTKDEEMVVAVGNQVLYGKYAGTELEWEGEKYLIMRQSDILAII"
     gene            7661..9292
                     /gene="groL"
                     /locus_tag="PKH68_04050"
     CDS             7661..9292
                     /gene="groL"
                     /locus_tag="PKH68_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNX12142.1"
                     /translation="MAKEILFNIDARDQLKKGVDELANAVKVTLGPKGRNVILEKKFG
                     APHITKDGVTVAKEIELEDSFQNLGAQLVKEVASKTGDDAGDGTTTATVLAQSIVSVG
                     TKNVTAGANPMDLKRGIDKAVEAVVENIKSQSRTVGDNYDKIEQVATVSANNDARIGK
                     LIADAMRKVSKDGVITIEEAKGTQTTIEIVEGMQFDRGYISPYFVTNTEKMSVEMEKP
                     YILIHDKKISNLKELLPVLEPAVQSGRPLLIIAEDVESEALTTLVVNRLRASLKICAV
                     KAPGFGDRRKEMLEDIAILTGGTVISEEKGIKLENATLEMLGTAEKITVDKDNTTIVN
                     GAGNKEAIATRVTQIKSQIAATTSDYDREKLQERLAKLAGGVAVLYVGAASEVEMKEM
                     KDRVDDALHATRAAIEEGIVPGGGVAYIRAIEALNNLKADNDDEQTGIEIIKRAIEEP
                     LRQIVANAGKEGAVVVQKIREGKADFGYNARTDKYENFFEAGIVDPVKVARIALENAA
                     SIAGMFLTTECVVVEKKEENPAPQMPMGGGMGGMM"
     gene            9657..10682
                     /locus_tag="PKH68_04055"
     CDS             9657..10682
                     /locus_tag="PKH68_04055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNX12143.1"
                     /translation="MKIGILSSGGDCPGINATIRGVGKTAINNYGMEVIGIINGFSGL
                     LYKDIIPLPESSLSGILTLGGTILGTAREKEFRKILKSPDKKDQEMIKKSYKELGLDC
                     LVCIGGNGTQKTTWALSKLGLNVIGIPKTIDNDVFGTDITFGFSTAVDIATDAIDRLH
                     STASSHQRVMVIELMGHHAGWITLHAGMAGGADVILLPELGFDMDIVCEVLNNRKKNG
                     KSYSIVALAEGIEVEGGTGGHHPATYFAREIEKRTGIEARETVLGYIQRGGSPSSYDR
                     NLGTLLGGYAAKLINDKKFGRMVSVKGVKITDVSLEDVAGKLRIVEPNDPMVLQGKKM
                     GISFGIK"
     gene            complement(10786..11136)
                     /gene="rplT"
                     /locus_tag="PKH68_04060"
     CDS             complement(10786..11136)
                     /gene="rplT"
                     /locus_tag="PKH68_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNX12144.1"
                     /translation="MPRSVNHVASRKRRKRILSLTRGYFGGRRNVWTVAKNTWEKGLQ
                     YAYRDRKNKKRSFRALWIQRINAAARLEGMSYSKLMGALHKSGIEMNRKVLADLAMNH
                     PEAFSAIVKKAKNA"
     gene            complement(11242..11439)
                     /gene="rpmI"
                     /locus_tag="PKH68_04065"
     CDS             complement(11242..11439)
                     /gene="rpmI"
                     /locus_tag="PKH68_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNX12145.1"
                     /translation="MPKMKTNSGAKKRFALTGTGKIKRKHAYKSHILTKKTKKQKRNL
                     TQTGLVSSVDNKEVRSLLCLR"
     gene            complement(11471..11980)
                     /gene="infC"
                     /locus_tag="PKH68_04070"
     CDS             complement(11471..11980)
                     /gene="infC"
                     /locus_tag="PKH68_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNX12146.1"
                     /translation="MPNRINEKIKGVKEVRLVGDNVEQGVVTIEEALRKADEMELDLI
                     EISPTAVPPVCKIADYQKFLYQQRKREKEQKAKASKVVLKEIRFGPQTDEHDYNFKLK
                     HAINFLKEGYKVKAFVFFKGRSILFKEHGEVLLLRFANDLEEYAKVDQLPQLEGKRMI
                     IFFSPKKTK"
     gene            12332..12850
                     /locus_tag="PKH68_04075"
     CDS             12332..12850
                     /locus_tag="PKH68_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNX12147.1"
                     /translation="MKRVILSFSILLYTFSTSIIGQQTGGASYYGNKLHGKRTANGRT
                     YHRDSLTCAHRTYPFGTLLRVRNLKNDKEVIVKVTDRGPFVRGRIIDLSYAAAKQIGF
                     VAHGTAKVEVTIYDSLKSIEEIYRKYSPDLKRMKFYPDSMKFPDNSIIPGLNCYNIET
                     EQPQPTHFKPSK"
     gene            complement(12924..13427)
                     /locus_tag="PKH68_04080"
     CDS             complement(12924..13427)
                     /locus_tag="PKH68_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNX12148.1"
                     /translation="MGHHQIDRLDKKILQLISKDARIPFLEVARECNVSGAAIHQRIQ
                     KLRNLGIIKGSEFIVDTYKIGYQTCAYIGIVLTDIKKFDYVVAELKKIPEVVECHYAT
                     GKYSMFIKIYARDNRHLLQIILEHLTLIDGVGHTETFQLSLDEIFNRQLSVFEVGEGD
                     LDDVEEE"
     gene            complement(13564..14487)
                     /gene="rsgA"
                     /locus_tag="PKH68_04085"
     CDS             complement(13564..14487)
                     /gene="rsgA"
                     /locus_tag="PKH68_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNX12149.1"
                     /translation="MQGLVVKNTGSWYTVLADTGELVECKIKGNFRIRDIRTTNPIAV
                     GDRVEFIFTTDGTAMINEICNRKNYIIRRSSNLSKYSQILAANLDLVALIVTVNYPVT
                     STVFIDRFLATAEAYRVPACLVINKTDRYSDDELNYAQALKNLYETIGYPVFLISAVN
                     EDTLEELKSGLNQKITLISGNSGVGKSTLINMLAPHSEARTNIISEYHNKGMHTTTFS
                     EMFSLPAGGYVIDTPGIKGFGTIDMQEFEVGHFFKEIFQVSKNCHFNNCTHIHEPGCA
                     VLDAVENHQISQSRYQSYLSIMDDCKSGKYR"
     gene            complement(14583..15143)
                     /gene="frr"
                     /locus_tag="PKH68_04090"
     CDS             complement(14583..15143)
                     /gene="frr"
                     /locus_tag="PKH68_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNX12150.1"
                     /translation="MVDYKKHINDAEELMQATVEYLDEALAHIRAGKANTRILDGVKL
                     EYYGSLVPLTNVATVTTPDAKTIAIQPWEKSLISVIEKAILNSNVGITPMNNGEVIRL
                     GIPPLTEERRKQLVKQCRQEGEDAKISIRNARREAIEHIKKMVKEGLPEDMEKDAEEE
                     AQKIHDKYVKKIEELLAAKEKEIMTV"
     gene            complement(15210..15920)
                     /gene="pyrH"
                     /locus_tag="PKH68_04095"
     CDS             complement(15210..15920)
                     /gene="pyrH"
                     /locus_tag="PKH68_04095"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNX12151.1"
                     /translation="MIKYKRILLKLSGESLAAEKKQGIDIVRLEQYAAQIKEIAELGV
                     QVGIVIGGGNIFRGLSGASKGFDRVQGDQMGMLATIINSLALNSALQAVGSKSRVLTS
                     VRMEPIGEYYSKRYAVECLERGEVVIIAGGTSNPYFTTDTASSLRAIEIEADVMLKGT
                     RVDGVYTADPEKDKSAVKFEEISYDEIYNRNLKVMDLTATTMCKENDMPIYVFDMDTV
                     GNLKKVIRGEKIGTLVKN"
BASE COUNT         4849 a         3347 c         3238 g         4670 t
ORIGIN      
        1 tcgcaaccga taaagatcgc aaacgtctga aagaatccat gcagaaagcc atgcagcatg
       61 gcaatggcat cattatgctt ctcgaaaaag ataaagacga agttcgctat tttagtcgta
      121 aactgatgtg cccaactaca ggcatttcgt acgatgatcc ggcacctcac aatttctcgt
      181 tcaattcgcc ccacggttat tgccccaggt gtaaaggact tggttttatc aatgaaattg
      241 atatggctaa aatcattccc gacacatcgg tcagtatatt caacggtggt atcgccccgc
      301 tgggaaaaca taaaaacagc accattttct ggcagattga agctatactg gccaaatatg
      361 aatgcacgct gaaaacaccg cttgctgatg tacccgatga tgccattgat gaaataatga
      421 atggcaccga cgaacaactg cacattaaga atgaatcact gggcaactcc aactatttcc
      481 tgagttacga cggcatcatc aaatatattg aaatgcaaca ggaaaattca gcatcggcta
      541 ccgaacagaa atgggcaaat cagtattcta aaaccattaa atgtaccgaa tgtgacggac
      601 aaaggctccg taaagaatcg ttgtatttcc gtattcacga caaaaatatt gctgcgctgg
      661 ccgaaatgga tatctcggaa ctgtcagaat ggctgaaaaa cgtagacttt tttctgaccg
      721 aaaaacagcg catcatagcc accgaaattc tcaaagagct acagtcaaga ctgcaattca
      781 tgctcgatgt ggggctcaat tacctggcgc tcaaccgaag ttcacaatca ctttccggcg
      841 gcgaaagtca acgcatccgc ctggctaccc aaatcgggtc ccaattggtc aatgtgcttt
      901 atattctcga cgaacccagt atcggactac atcagcgcga caacctgcgg cttattcatt
      961 cgctgaagca acttcgggat accggaaatt cggtcattgt ggttgaacat gataaggata
     1021 tgatgctggc tgccgattat gtactggatt tggggccgcg ggcaggccga cttggcggcg
     1081 aactggtttt tgcaggtact ccgacagaaa tgcttcagtc cgaaaccctg accgccgatt
     1141 acctcacagg taaaaaacaa attgaaattc cggagaaaac ccgcgaaggt aacggaaaat
     1201 tcctgacact taaaggcgct aaaggcaata atctgaagaa tattgatgtt acttttccgc
     1261 tgggaaaaat gatttgcgtg accggtgtat cgggcagtgg aaaatccaca ctcatcaatg
     1321 acacgttgca acccattctg agccagcact tttaccggtc actacaggat cctcttcctt
     1381 acgacagcat tgatggggtg gataatatcg ataaaattgt acaggtggat cagtcgccca
     1441 ttggacgcac gccacgatcc aatccggcga cctacacggg tgtattcagc gatattcgca
     1501 atctttttgc caccttgccg gaagctaaaa ttcgtggtta taaacccgga cgattttcat
     1561 tcaacactgc cggtggtcga tgcgaaacat gtggaggaaa cggttataaa acgattgaaa
     1621 tgaacttttt gcccgatgtg tatgttccct gcgaaagctg caacggcaaa cgctacaacc
     1681 gcgaaaccat ggaagtgcgt ttcaagggaa aatcgatagc tgatgtcctg aacatgacca
     1741 ttaaccaggc ggtggaattt ttcgaaaatc aaacttatat tctcaacaaa ataaagacac
     1801 tacaggatgt aggcttggga tatatcaaac tgggacaaag cagcacaacc ctctccggtg
     1861 gcgaaagtca gcgtgtgaaa ctggctaccg aactatcgaa acgcgatacg gggaaaacac
     1921 tctacgtgct ggacgaaccc actaccggac tgcattttga agatatccgg gtacttctgg
     1981 gtgttctcaa tcggttggtt aaccgtggaa atacggttat aattatcgaa cacaatatgg
     2041 acgtaattaa aagcgccgac tacatcatcg acattggtcc cgaagggggc aatgggggcg
     2101 gcagagtaat ttgcaccggt agtccacagg aggtagccgc taacaccgaa agtgaaacag
     2161 gaaaatttct cgcacaggaa ttacatctgc attcctgatt ttgaattagt accctaaatg
     2221 cttatctttg gaataaatgt gcattttaaa ggatttaatt tttaactttg caactaagac
     2281 ataattcttt ttttccaatt aatacaagta aaaaaaatat gaaccaaaaa ctaaaattat
     2341 ttctatacgg actggttgtt tttttcgttt acgctgtact cacctgggtg ctgaaactca
     2401 tcgcccacaa aatgcctgaa ggtgaagcat ttctgggtgt atataccagc actgatcttt
     2461 ggctgggtct ggttattgca atcatactca cctattccca ctacatgaag atgaaaatca
     2521 aataaagaaa tgtcggaaga tcaattatct gaaataacag aatatatcat tcgctttctg
     2581 ctcggcagca aggccaatga aacattcagc aatctgattg gctatacttc cgataaaaca
     2641 cttttcagta aatacaaaat agttatcctc ccttccggtt tttttgatca gggattttat
     2701 ggcactgcac aatcacttcc acagttcccg ctgcctttgt gggatgatat gccactgctt
     2761 tatggtcagc ctgaaattgc gaaaaacggt gataccatca ttttacatgc cgatattatt
     2821 gtcagcactt tttttctgat aagccgctac gaagaaacaa tccggcggaa tgaaagagac
     2881 gaacatggtc gttttcccgg cagcaaatca ttacctttca gggcgggatt tttacatcgc
     2941 ccggtagtgg atgaatacgg caagcaactg cgaaaactgc tgagagaaac cggcgcagtg
     3001 ataaacgaac cggaagagaa attcaataaa atatatctca cacatgatgt ggatcagctg
     3061 gctcactatc gtaatatccg agggataggc ggtgctgtta gtcgtttttt caaaaacccg
     3121 gaacaaactt tcaaggcact gcaatcctat tttttgggac ttaagtatga tccctggttt
     3181 acatttccgt ttctgattga tgaaaacaga aaacttagaa atgaactcgt tgaaacaata
     3241 ttttttatca aaaccggcgg tggaaattta caaaccgata aaccactcca tgatattttc
     3301 gataaaaatt ttcagatatt gtttcgttta ggaatagaaa atggagtaaa attcggactt
     3361 cacccatcct atcaggccgg gattgagccc cgattaatca cggatgaaaa aaatctgttg
     3421 gataaaagac taaatcagca gacaagctac tccagaaatc attttttggc aagccgcgag
     3481 cccgaagata tgcaggcatt gattgacaat ggttttacag atgattacac aatgtcgtat
     3541 gccgatcagg caggtttcag actgggaacc agccgggctg tgaaatggat aaatccgaca
     3601 atcctgcaac tgacaaacct aacattacat ccactcacac tcatggacag tacgctgagt
     3661 gacaaacgat acatgaacct cattgtggat gaagcatttt ccttttcaaa aaaaatgatt
     3721 gatcaggtga aaaaaaacaa tggtgatttg actttgctct ggcacaatac ctcggtggaa
     3781 aaacataacg ggctttatca ccgagatttg taccggtgga tgattaacta cctgaaaatg
     3841 caggcctgat ggtttgtgaa aaagcataaa tacacgaaac gatgaaaatt ctgataattg
     3901 gagaaccttt tttgccacct gcgtatttgc cgcgggttcg gtatttttgt tcctatttta
     3961 tcgaaaaagg atggaaggtt gatttggtta cagagaattc tgaacacttg cattacgttc
     4021 ccgaaaacgt tacactgtta ccgataaatt actataaata caagcaggga attattgcca
     4081 aatctgaatg ggcatttaaa ttcttactgg atttgtgcct ggattataag ggacgctatt
     4141 tttataaaaa atcgaaggaa ttactgagcc aaaatcatta cgacctggtt ttttgcagtt
     4201 ccagttttac ttttccgtta actgtggccg cgagagttgc cgaaaaactc aacagaccct
     4261 tgtttgtaga tttgagggat attgcagaac aatcgcccga cgacaaccat tatattgccc
     4321 atcggccacc gccattgttg ggaaaactga ttacccgtat ttataaaaat gtaagcatta
     4381 acagacgcaa cagggtattg cggttagcca ccggagttac taccgtttca ccctggcatg
     4441 ttcaaacatt atcaaagcac aatccgtata cacacctgat ttacaacgga tttgacgaga
     4501 atatgtttgt tcctggcaag ctggcaaccg aacggtttac catctcctac tttggacgga
     4561 tttacaacga acaaatacgt aatccgcgat tgcttttttc ggctttgagt gaattaaaca
     4621 aaaaaggaat ggtcacaccc gaaaatacgg tggttaagtg gtttgtggat gagcagtcgc
     4681 gcaacgtaat actcaatata gccaacaact ataatttgtc gggtttcatg gaatttcata
     4741 actttataac tccgaatgcg ttatccgatg aaatgaacaa aagttcggtg ctgctggttt
     4801 tgtgtaacgc gaaggacaca aaaaactatt ttggaataat gaccaccaaa ttctttgaag
     4861 cattgggtac taatcgtccg gtagtatgca ttcctgataa taacgacaat ctatctcatt
     4921 taataaaaga gacaaagtgt ggtcttgtca gctctgatga attggaagta gaaaaatttt
     4981 tgattgaaaa actggaggaa tggcagcaaa aaaaacgtac caacggcatg ttggacgaaa
     5041 cgatacgaat gaatttttcc agaaaaaaag gagcagaaac acttgaaaaa ctttttataa
     5101 cagccataaa acaacaataa tgaatataaa aaaactggta ccacgccctt tgatcatgat
     5161 gcgccgcgag atgcttcctg gctggcgaaa aattacaaag aaaaatatgc agcaaaaaaa
     5221 gatgatcccc attctgcacc tacatcttgc tgatcattgt aatctcgact gccgcggttg
     5281 cgacaatttt tcgcccctgg ctcccgaagt ttttgccgat gtggacgttt tcgaaaaaga
     5341 ttgtgccaaa atggcagacc tttcacaggg acgaattgaa gaaatacaac ttttgggagg
     5401 tgaaccgctg cttcatcctc aaatcaatac ttttatggaa attgtacgcc gctactttcc
     5461 aaccaacaaa ataagcatag taagcaatgg cattttgttg cagaccaaaa aggatgtatt
     5521 ttgggaagca tgccggagat ataagattga gattatcgtt acaaaatatc ccattcagct
     5581 tgattttgaa aaaattaagc aaaaagttct ggcacaaaac gtacctttct ctttctatgg
     5641 aaataccgaa acggtggaaa aaacaatgga atgtcttcca ttggatttgg aaggcaaaca
     5701 agatccaaaa gacagcttcc tgcgatgtgc ccgtgccaat cggtgcgtat cgctggataa
     5761 tggtaaaatt tatacctgtt cgcttattcc gtatgtaaaa tactttaatg accattttca
     5821 acaaaatctg actgttacca cggccgatta tctcgagctg catcaagcta cgagttacga
     5881 tgaaattttg aattttgttt gtaaaccaat gccgttctgt cgctattgca acataaaagg
     5941 aacaatctgg gatattggtt acggcatttc gaataaagac attagcgaat ggacaggtaa
     6001 ataaccttga attttcatgt gtttataaat tatatatctg ccccctaacc ccctaaaggg
     6061 ggaatattga gcgtctatca tttgttgtga gttttttaat taaaccgtgt gtgtcaaaaa
     6121 tattttactt ggacatgtgt gtttcatctt atgatttcta ttttttttca atttctgact
     6181 tctaatttct aatttctaat tttttctaaa agtgttatcc atccttattc ccacatataa
     6241 ttacgacgtg acccggttgg taactgagct tcaccgtcag gcactgaaca cgtatgtgga
     6301 ttttgagatt ttggtgatgg aagatggttc tactcaattt ctggaggaaa ataaggcgat
     6361 atccaatctt caattttgtc attacaccgc tttatcacac aatatcggtc gctcagccat
     6421 tcgcaatcgc ctggccgacg aagcccgata cgggcatctg ctttttatcg attgcgatgc
     6481 tgaagttcat acctcacatt ttgtagaaaa atacctggct ttttgtcgtg aagaagtggt
     6541 agtaattggc gggacggctt atgaacctga aaataatgac cctgacttca gtttacggct
     6601 gaaatacgga cgcatgcggg aagccaaatc ggctcaaaat cgagaacaag ccggaaaata
     6661 ttcacatttt tcttccttca actttcttat ttccaaatca attttcaacc aaatccggtt
     6721 cgatgaaagc atacggggtt acgggcacga ggacactctt ttcggacatc agattgccga
     6781 actccactgc gaagttcacc acatagacaa cgatctgata cataaagggc tggatgacaa
     6841 tattttattt atccataaga cagaagaaag taccaaaaat ctttatttgt tgcataaaag
     6901 caacaaatat cccttcctcg caaatcagtc aaaactcctg cgtacgttta tcaatctcga
     6961 tcggaaaaat ctatcacccc tacttgctaa actttttgac gtactccaaa cttgtatgca
     7021 gaaacaattg tgcagcagtc ggccttcact gaaaatctac gatttatata aacttctctt
     7081 tcttgccaaa atttcacttt ccgactgaca acttgtcagt ctattttagt tttttttaat
     7141 ggcatactgc attttctgcc aaaatttctt cttttggctt cttggcacag atttcgcaag
     7201 gttttttaca gccgataaat agaaatatca acatataatt atacaattaa aatattacaa
     7261 ctatgaacat taaaccatta gcagaccggg tgctcatcaa accagcacct gccgaagaaa
     7321 aaacagtaag tggaatcatt atccccgatt cagccaaaga aaaacccctg aaaggtgaag
     7381 ttttagccgt tggcaatggc acaaaagacg aagaaatggt agttgccgtt ggcaatcagg
     7441 ttctttatgg aaaatatgcc ggaaccgaac tcgaatggga aggtgaaaaa tacctcatca
     7501 tgcgtcagtc tgatattctg gcaataattt aaacaattgc cccctacccc cttaaggggg
     7561 aacttatgag caaatattta aacaaaaaac aataacaatt aatttataaa atccgtacct
     7621 cccgcggttc tcccccttca gggggtcggg gggcttatct atggcaaaag aaattttatt
     7681 taatattgat gcccgcgacc aactcaaaaa aggagttgac gagttggcaa atgcagtgaa
     7741 agtaacactt ggaccaaagg gtcgtaatgt tattttggaa aaaaaattcg gcgcacctca
     7801 cattacaaaa gatggtgtaa ctgttgcaaa agaaattgaa ctggaagatt catttcagaa
     7861 tttaggagca caattagtga aagaagtggc ttcgaaaacc ggtgacgatg caggtgacgg
     7921 tactacaact gccactgtac tggcacagtc tattgtaagt gtgggtacaa aaaatgtgac
     7981 tgccggagct aatccaatgg atctgaaacg cggtatcgac aaagcagtag aggctgtggt
     8041 tgaaaacatc aaatctcagt caagaacagt tggtgacaac tacgataaaa ttgaacaggt
     8101 ggccaccgta tcggccaata acgatgcccg tatcggtaaa ctgattgccg atgccatgcg
     8161 taaggtatcc aaagacggtg taattactat cgaagaggcc aaaggtacac agacaaccat
     8221 cgaaattgta gaaggtatgc agttcgaccg tggctatatt tcgccctatt ttgtaacaaa
     8281 caccgaaaaa atgtctgtgg aaatggaaaa accatacatc ctgattcacg acaaaaaaat
     8341 ctccaacctg aaggaacttc ttccggtgtt ggaacctgca gttcagtcag gacgcccatt
     8401 attgattatt gccgaggatg ttgaaagcga agcacttacc acactggttg tgaaccgtct
     8461 gcgtgcttct cttaaaattt gtgcagtaaa agctcccgga tttggcgacc gccgaaaaga
     8521 aatgctcgaa gatattgcca ttcttaccgg tggtaccgtt atttcggaag aaaaaggcat
     8581 taaactcgaa aatgcaacac tcgaaatgct tggcacggcc gaaaaaatca ccgtggataa
     8641 agacaacaca accatcgtaa acggtgctgg caacaaagaa gctattgcaa cccgtgtaac
     8701 ccaaataaag tcacaaattg cagctaccac ttcggattac gatcgtgaaa aactgcagga
     8761 acgtctggca aaactggccg gtggtgtggc agtactttat gtgggagctg cttcggaagt
     8821 tgaaatgaaa gaaatgaaag accgcgttga tgatgcactc catgcaacac gcgcagctat
     8881 cgaagaaggt attgtacccg gtggcggtgt ggcatatata cgtgccatcg aagcgctgaa
     8941 taacttgaaa gccgacaatg atgacgagca aaccggtatt gaaatcatca aacgtgctat
     9001 cgaggaacca cttcgccaga ttgttgccaa tgcaggtaaa gaaggtgctg tggttgtaca
     9061 gaaaatccgc gaaggtaaag ccgattttgg atacaatgcc cgcaccgata aatatgaaaa
     9121 cttctttgaa gccggtatcg ttgatccggt gaaagtagct cgtatagcac tcgaaaatgc
     9181 agcatccatt gcaggtatgt tcctcacaac cgaatgtgtg gtagtagaaa agaaagaaga
     9241 aaatcctgct ccacaaatgc ctatgggcgg tggaatgggc ggtatgatgt agtagccact
     9301 ccaaacctct acgcagggaa ctttaataaa actcctcaaa tcaattctga tttggggagt
     9361 ttctttttgt gaagagaact cttgaaatta gtcaatgact gtttcaggtt tggatgattg
     9421 atttctatcg aatattatta atcacaagtt tttaagataa aacacattaa aaatgaataa
     9481 tatccctgct tttttctttt ctttgtaaaa acaaattcta ttgacgtgaa acgcccatgt
     9541 ccaactaaaa aaaaaatgga cacacaacgg tatgattaaa taacgcataa caaatgatag
     9601 acgctcagta ttcccccttt agggaggtag ggggccgata aacaaattct aatcatatga
     9661 aaataggtat tctctcttca ggtggcgatt gtccgggtat aaacgccacc atccgcggtg
     9721 taggcaaaac agccatcaat aactatggca tggaagtaat cggtatcatc aatggttttt
     9781 cgggcttatt gtacaaggat attattccac tgccggagtc gtcactgtcc ggtattctca
     9841 ccctgggagg aacaattctg ggtaccgccc gtgaaaagga atttcgtaaa atcctcaaat
     9901 ctcccgacaa gaaagatcag gaaatgatta aaaaaagtta caaagaactt ggactcgact
     9961 gtctggtttg tataggaggc aacggcactc agaaaacaac ctgggcattg tcaaaactgg
    10021 gattaaacgt cattggcatt cccaaaacca ttgacaatga cgtttttggt actgatatta
    10081 cttttgggtt cagcacagcg gttgatattg ccaccgatgc aatcgaccgg cttcactcca
    10141 cggccagttc acaccagcgg gttatggtca tcgaactgat gggacaccac gcaggctgga
    10201 ttaccctgca tgccggaatg gcaggtggtg ccgatgttat tctgctgccc gaattgggtt
    10261 ttgatatgga tatagtttgc gaagtactaa acaaccgcaa aaaaaacggc aaatcctatt
    10321 ctattgtcgc actggccgaa ggtattgaag tggaaggtgg aaccggtggc catcatccgg
    10381 ctacctattt tgcacgtgaa atagaaaaac gcaccggaat agaagctcgc gaaactgtac
    10441 tcggttatat tcagcgtgga ggttcaccct catcgtacga ccgcaatctg ggcaccctgc
    10501 tcggaggcta tgcagccaag ctgattaacg ataaaaaatt cggacggatg gtttcagtca
    10561 aaggcgtgaa aattacagat gtttcgctcg aagatgtagc cggcaaactt cgtattgtag
    10621 agcccaacga cccaatggtg cttcagggta aaaaaatggg gatttcattt ggaataaaat
    10681 aaatcagaaa agaatatgaa ataaaaaacc gcaattcctt tacaggttgc ggttttttta
    10741 tctaaaagaa actgttttat agcagttctt caaatggttg aatgattaag cgttttttgc
    10801 tttctttaca atagcgctga atgcttcggg atggttcata gccagatcgg ctaatacctt
    10861 gcggttcatt tcaatacccg atttgtgtaa tgcacccatc aatttggaat acgacatacc
    10921 ttcgagacgt gcagcagcat tgatacgttg aatccacaat gcgcggaaac tgcgtttttt
    10981 attcttacgg tcacggtaag catattgaag acctttttcc caggtgtttt tggctactgt
    11041 ccatacattg cggcgtccgc cgaaataacc tctggtgagg cttaaaattc tttttcttct
    11101 tttacgtgaa gcaacgtgat ttactgatct tggcataatt taatgaattg tgaacgttag
    11161 cgccgcattt tcttgcgatc tttcaggcta aacactcgat tttacaataa aagaaaattt
    11221 gtttctaaag ttaattttga cttatctcaa acacagtaat gatcttacct ctttgttatc
    11281 tacactgctc accaatccgg tttgcgtaag attacgtttt tgttttttgg tctttttagt
    11341 caaaatgtga cttttgtaag catgttttct cttgattttt cctgttccgg taagagcgaa
    11401 tctttttttg gcaccggagt tagttttcat ttttggcatt gtctcttatt tatatattat
    11461 tgaaattcaa tcattttgtt tttttgggag aaaaaaagat aatcatcctt ttaccttcca
    11521 actgtggcaa ctgatccact ttggcatatt cttccaaatc attggcaaaa cgcaaaagca
    11581 atacttcacc gtgttctttg aacagaatag aacgcccttt gaaaaacacg aatgccttta
    11641 ccttgtaacc ttctttgaga aaattaatgg cgtgcttcaa cttgaagttg taatcatgct
    11701 catcggtctg aggtccgaac cggatttctt tcagtacaac ctttgaagct ttggcttttt
    11761 gttctttctc acgcttccgt tgttggtaca gaaatttctg ataatcagca attttgcata
    11821 ccggaggcac cgctgtggga gatatttcta tcagatccag ctccatttca tctgctttgc
    11881 gcagtgcttc ttcaatggta acgacgccct gctccacatt gtctccaaca agtcgtactt
    11941 cctttacacc cttgattttc tcattgatgc gatttggcat ttccttttct ttgtgaccct
    12001 gccgcggctt gataaataat tgtttagcta ttgcttgttt cctcccgaaa attttagtga
    12061 aacttattgt tacttttttg agcgtgcaaa tataacacat ttttttaaat aatgaaaata
    12121 aagcacctat gaatttagca aaacgaaccc ggattgtttt tcagttttgc ttatacccga
    12181 ttagcaaaac aaatgaagcc atttttcgtt atattaaaga tgatacacat aacaatctgt
    12241 tagttagccg tataattttc aattcaaaaa aggcacaata tttgcaatgg gattcagctc
    12301 tgttttcaat tttccaataa gcgtttattt tatgaaaaga gtaatacttt cattttcaat
    12361 acttttatat accttttcca cctctattat aggacagcaa accggtggag catcgtacta
    12421 cggcaataag cttcatggca agcgaacggc taatgggagg acataccacc gcgatagcct
    12481 cacctgtgct catcgtacct atccgtttgg cacattgctc agggtacgta acctgaagaa
    12541 tgacaaggaa gtaatagtca aagttactga tcggggaccg tttgtcagag gcagaatcat
    12601 tgatttgtcg tacgctgcgg ccaaacagat tggttttgtt gcacacggta ctgctaaggt
    12661 agaagttacc atatatgatt cactgaaaag tatagaggaa atttacagaa aatacagtcc
    12721 tgacttaaaa agaatgaagt tttatcccga ttctatgaaa tttccggata attccattat
    12781 tcccgggtta aactgttaca atatcgaaac cgaacaaccc caaccaacgc attttaagcc
    12841 ctcaaaataa ttgaaaataa ttggtaatga aaatagccgt atgaatctta cggctattta
    12901 tgtttaagta tttctaaaaa cgattattct tcttccacat catccaaatc accctctccc
    12961 acctcaaata ctgacaattg acgattgaat atttcatcga gcgacaactg gaaagtctct
    13021 gtatgaccca caccatcgat tagcgttaga tgttccagaa taatttgcaa caaatgacgg
    13081 ttatcacggg catatatctt aatgaacatg gagtatttac cggtagcata atgacattcg
    13141 actacttcag gtattttttt aagttccgca acgacataat cgaatttttt aatatcagtg
    13201 agtacaattc caatatacgc acaagtctga tatccaatct tgtaggtgtc aacaataaat
    13261 tcggaaccct taataatgcc caggttacgt agtttttgta ttcgctgatg gatggctgca
    13321 ccggaaacgt tacactcgcg agcaacttcc aaaaaaggta ttctggcatc cttggatatt
    13381 aattgcagga tttttttatc cagtctgtca atttgatgat gtcccatttt aatttatgtt
    13441 tttgtgtaat ttctttataa atattttgaa tactgatatt aaccgacaca aaaatatcca
    13501 attttattga tttatcccaa caaaatgcta cattttatta cttcatttca aaaaacgatg
    13561 gaattacctg tattttccgc ttttacagtc gtccattatg ctcagataac tttggtagcg
    13621 ggattgactg atctgatgat tttcgacagc atcaagtacc gcacaaccgg gttcgtggat
    13681 atgtgtacag ttattaaaat gacaattttt agaaacctga aatatttcct tgaaaaaatg
    13741 acccacttca aattcctgca tatctattgt tccaaaacct ttaatgcccg gtgtgtcaat
    13801 cacatagcct ccagctggga gcgaaaacat ttcagaaaaa gttgttgtat gcattccctt
    13861 attgtgatac tcggaaatga tgttggttcg tgcttcggaa tgaggagcca gcatattgat
    13921 gagggtcgat ttaccgactc ccgaattgcc cgaaatgagt gtgatttttt ggtttaatcc
    13981 gctcttaagt tcttctaagg tatcttcatt aactgccgaa atgaggaaaa ccggatatcc
    14041 aattgtttcg tagagatttt tcagtgcctg agcataattc aattcatcgt cagaatatct
    14101 gtcagtttta ttaatcacca gacaagcagg aacgcgatat gcttcggcgg tggccagaaa
    14161 acggtcaatg aatacggtag aagttacggg gtaattcacg gtaacaatga gtgcaaccaa
    14221 atccagattg gcagccagaa tttgtgaata cttcgaaaga ttggatgaac gacggatgat
    14281 gtagtttttt cggttgcaga tttcattgat catagcagtg ccatcggtgg tgaatatgaa
    14341 ttccacccta tcgccaacgg ctatgggatt ggttgtacgt atatctctga tacggaaatt
    14401 tcctttgatc ttacattcca ccaattcacc cgtatcagcc agtacagtgt accaactgcc
    14461 cgtatttttc actacaagcc cctgcatgat ttagttatat tctgataaaa aatttcggtc
    14521 atcccgtaaa tccgggacga ccgccaatcc tattctcacg tactcccggt ctacaatcgg
    14581 ggttacacag tcataatctc tttttctttg gctgcaagga gttcttcaat tttcttcacg
    14641 tatttgtcgt gaattttctg tgcttcttct tcagcatctt tttccatatc ttccggtaat
    14701 ccttctttca ccattttttt gatatgttct attgcttctc ttcttgcatt tcggatgcta
    14761 atctttgcat cttcaccttc ctgtcggcat tgtttaacca gttgcttacg ccgttcttcg
    14821 gtgagtggtg gaattcccag cctgataact tcaccattat tcatgggtgt aattcccaca
    14881 tttgagttca aaattgcctt ttcgattacc gaaatcaggg atttttccca cggttggatg
    14941 gcaattgttt ttgcatcggg agttgttacg gtggctacgt tagtcagggg taccagagaa
    15001 ccgtaatatt ccaacttcac tccatcgaga atacgggtat tggcttttcc tgcgcggata
    15061 tgtgccaatg cttcatccag atattctacc gttgcctgca tcaattcttc ggcatcattg
    15121 atatgttttt tgtaatcgac cataatgtta attatttagc ctcttaattc cttcaaagaa
    15181 ggacttaagt atatgttaat aatattctct taatttttta ccagtgtacc gattttctct
    15241 cctcttatga cttttttcag attacccacc gtatccatat caaaaacgta tatcggcata
    15301 tcattctcct tgcacatggt agtagccgtg aggtccatca ctttcagatt gcggttatag
    15361 atttcgtcgt aagaaatttc ttcaaatttc acggctgatt tatctttttc gggatcggcc
    15421 gtgtacacac cgtccacacg tgttcccttg agcataacat cggcttctat ttctatagcg
    15481 cgcagcgacg aagcggtatc ggttgtgaaa tacggattac ttgtaccacc tgcaattatt
    15541 acaacttctc ccctctccag gcattccacg gcgtaacgct tgctgtaata ctcgccaata
    15601 ggttccatcc gcactgaggt gagtacacgt gacttggatc caacggcctg aagagcagaa
    15661 ttcaatgcaa ggctgttgat gattgttgcc aacattccca tctggtcacc ctgtacgcgg
    15721 tcaaagcctt tggatgctcc gctcaatccg cggaatatat tgcctccacc tattactatc
    15781 cctacctgca ccccaagttc agctatttct ttaatctgag ctgcatattg ttcgagccga
    15841 acgatatcaa tgccttgttt tttttcggct gcaagcgatt caccgctcag ttttaggagg
    15901 atgcgtttat attttatcat tgcctttgac agtaattatt tcaaaacaaa agtaagaaaa
    15961 taggttggta tttgcaaatg tgttttgaat tgttgtttga agtaaaataa attaattgat
    16021 cttctgaacc ttactttata acgcgggcta aaatttctga tttaaaaaaa tgttggtatc
    16081 gtgagttgta ttgttcttaa aaca
//
LOCUS       DAOPZK010000042        15754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_3995_length_15754_cov_23.335053, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000042 DAOPZK010000000
VERSION     DAOPZK010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15754
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3995_length_15754_cov_23.335053"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..442)
                     /locus_tag="PKH68_04100"
     CDS             complement(134..442)
                     /locus_tag="PKH68_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HNX12152.1"
                     /translation="MSSIKYEKETVEKMIGLYCSRKHGTDRGLCAECEAVNDYASARL
                     DKCPFGDDKKACSKCKVHCYKPEMRARIREIMRYSGPRMLLYHPFDYLKHLLKDKKES
                     "
     gene            complement(459..782)
                     /locus_tag="PKH68_04105"
     CDS             complement(459..782)
                     /locus_tag="PKH68_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNX12153.1"
                     /translation="MEKIYYSISEVAEMFQINQSNLRFWEKEFKQLKPRRNEKGTRFY
                     SKDDLQLIKQIVFLVNDQKLTLEGVRKKLSDKKDVVAKQQEIKERLTTVREELKSMIA
                     LIDKK"
     gene            complement(794..2083)
                     /locus_tag="PKH68_04110"
     CDS             complement(794..2083)
                     /locus_tag="PKH68_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNX12154.1"
                     /translation="MTKSHVFHILLFLFLCLRGVAQNDNLKANDTGFSPAVSADSALI
                     VPEDFSGSLDHLLHSWAVDKDTPPDCWSQTNPATTDEQYRERLAKLPHEIEMPFNEEV
                     KSFIEIYTRKNRREVEYMLGMSRHYFPIFEEALEKEQLPLELKILPIIESALNPTAVS
                     RSGATGLWQFLIGTGKMYGLEVNTLVDERMNPVASSKAAVQYLKELYSIYSDWHLVIA
                     AYNCGPGSINKAIRRAGGKPDFWAIYPYLPVQTRAYVPIFIAANYTLNYAAQHNLCPV
                     KVRIPALTDTIMINTRMHFEQISAILKLPVEELRLLNPQYRRNIIPGDIKPYPLCLPQ
                     NYAGLMIDKKDEILAYRADVLINNRRAEIEQVKPTTTAKTSEQKKEQSTLHKVKRGQT
                     LGGIAEKYNVSVSNLKKWNNLGNSKIKTGQVLKIRHS"
     gene            complement(2298..3032)
                     /locus_tag="PKH68_04115"
     CDS             complement(2298..3032)
                     /locus_tag="PKH68_04115"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNX12155.1"
                     /translation="MIFKKNKLKKTLVTNLFLLFFIIPGFAGVNICIENNDLNIAVAV
                     DDTTTLTPMDIQPSNKYMNMPPVMADTVVVSSLEKPKFKPDPVRSIWMGAAIPGFGQI
                     VNHKYWKLPIVYGGYLGFAYAISWNNSNYITYKNAYRDIIDGDPTTNSHIDILPRGYT
                     VQMIGEGTYTNILKTRQSTFRQWRDLSIILSVGYYALVLLDAYVDAQLYDFDISPDLV
                     LNVQPTRIERERQSPPAYGMHWSIKF"
     gene            complement(3029..3907)
                     /locus_tag="PKH68_04120"
     CDS             complement(3029..3907)
                     /locus_tag="PKH68_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNX12156.1"
                     /translation="MAKNTGLGRGLGALIDIGSVSTSGSSSINEVEIALIYPNPNQPR
                     THFDEESLTELANSIRELGVISPVTLRKNDDETYLIIAGERRFRAAKSIGLTSIPAYV
                     KTAADEEVMEMALIENIQREDLNAIEIALTFYRLMEEYKLTQDRLSDRVGKKRATIAN
                     YLRLLRLPAEIQMGLKNKKIDMGHARAILGLSEPAVQLKLYDLILKKDLNVRKVEEMV
                     RNFLANGKFEGNPGQGKTNIVKDLKELNDLKSQLSTIFDTKVQMSVDQKGKGKITIPF
                     ANDDELMRIMQLIDKI"
     gene            complement(3932..4693)
                     /locus_tag="PKH68_04125"
     CDS             complement(3932..4693)
                     /locus_tag="PKH68_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008565334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX12157.1"
                     /translation="MGKIISIANQKGGVGKTTTAINLAASLASLGKKVLLVDADPQAN
                     ASSGLGIDVRTVEFTVYECLIDNVPSPQAIHQTEVENLFILPSHIDLVGAEIELLNLE
                     KREHVMEKMLKPIKNDYDFILIDCSPSLGLITVNALTASNSVIIPVQSEYFALEGISK
                     LLNTIKIIKNKLNPILEIEGFLMTMYDNRLRSANQVFAEVKKHFDNLVFDTVIYRNVR
                     LSEAPSYGKPISLFDNESRGSVNYQDLAKELITRQ"
     gene            5019..>6173
                     /locus_tag="PKH68_04130"
     CDS             5019..>6173
                     /locus_tag="PKH68_04130"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX12158.1"
                     /translation="MKLQRYILLLFVVLLAFFSCREDLVVPTVDPPKPDNPTVDIITH
                     SDGLQYVFDIDALPEIYLEISTAEWNRLLSNFDINRDNEEYIQSNCVFVKNSRRDTLN
                     NAGLRLRGNTSRRRPEGTQGQKHNSSNPVWHHVHFTLNFEKYDKKQDLHGLEKITLKW
                     FKDDGVYAREVYCYDLFERFGVWTSPKSSYCKLYLRITGDNRQVYYGVYQIVEQIDKQ
                     YIKNRSGKFAGNEGNLWKATWGADFKNADKNKMGVENITLNPATMVQPVYDLKTNEAS
                     LEAAKTQLEAFINYFNRKTGDDFKTWIAQRMDVPLFLKTYAVSTLCGMWDDYWNNSNN
                     FYFYFDTDDRFYFIPYDYDNTLGTSLLMANSGTQDLLNWGQSTNPLVAKII"
     assembly_gap    6174..6273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6305..7987
                     /gene="gldG"
                     /locus_tag="PKH68_04135"
     CDS             6305..7987
                     /gene="gldG"
                     /locus_tag="PKH68_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNX12159.1"
                     /translation="MKKISKSTYIIFVAIAALTVVSSLFFYRIDLTTDKRYSISPQTK
                     TLMHSVQEPVTVVVYLTGDLNPGFLRLKRATVELLDELNIYSKKQFNISYINPSEAAT
                     SEEREQNYLHLEQRGLTAASVYEKDKEGKNIQKIIYPWLEISYRGKTIPVNLLKNIPG
                     YSGEENLNISIENLEFELTDALRRLINTKVEKIAFLEGHGELSEEETLDISQSLSRYY
                     QIDRGTIGTDPAVLNDYKAVIIAKPTLPFSEKDKFVIDQYIMRGGRVLWLVDGVRVSP
                     ESLSTGGFTPAVELDLNLSDMLFRYGVRISPVLVEDVQSVLIPVNVAAKGEKPQFQPT
                     PWVYAPLLLTSGDHPVTRNLAPVKANFASAIEMVGDDKKLERTFLLASSDNTHILQTP
                     AEISLAAMPDLTDKNYFNRSYVPVGVVLEGEFQSDFTNRMPPEEIVNPPAIQTKSVPG
                     RQIVIADGDIIRNETVGTGGNKKVIPLGFDRYMNQQFGNKDLITNAVLYLTDKNGWME
                     LRSRTVALRLLNKKISAIEKTKWQIINVVIPPVVLLLAGLLYQWIRRRRYAR"
     gene            complement(7991..9331)
                     /gene="argH"
                     /locus_tag="PKH68_04140"
     CDS             complement(7991..9331)
                     /gene="argH"
                     /locus_tag="PKH68_04140"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNX12160.1"
                     /translation="MIMKLWEKSTQVNKKIEKFTVGNDRMLDIYLAKYDILGSMAHIL
                     MLQSVGLLSREELDVLLRELKNIYQLAVNDDFIIEEGVEDVHSQVELMLTRKLGDVGK
                     KIHSGRSRNDQVLVDLKLYTRAEIEKTVDAVSALFEVLIEQSEKYKHILLPGYTHLQI
                     AMPSSFGLWFGAYAESLADDLQMLLAAWKITNRNPLGSAAGYGSSFPLNRQMTTDLLG
                     FDTMNYNVVYAQMGRGKMERIVATALAGVAATVSKLAFDACMFTSQNFGFIKLPDEFT
                     TGSSIMPHKKNPDVFELTRAKCNKLQSIPQQISLITTNLPSGYFRDLQIIKEVFLPAF
                     GELRDCLEMAAMMMERVDVKTDILDDPKYDLIFSVEEVNRLAQNGMPFRDAYKKVGLD
                     IEAGNFNPDKNIHHTHEGSIGNLCNDRIKIYKDSIREQFNFSGVHEAEQNLLNS"
     gene            complement(9415..9858)
                     /locus_tag="PKH68_04145"
     CDS             complement(9415..9858)
                     /locus_tag="PKH68_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNX12161.1"
                     /translation="MTTYESDVKTISSSGEVVFNQLSKLSNFSNLQNMNAEAQAKLNE
                     MLKDVEFDDDSCRFSVEGIGKVGFRITEREPFKTIKLEAENSPIAVNGWIQLVQVAEN
                     DTRMKLTLKADMPMMIKMMADSKLKKGINMIADALVQFLSMKTAK"
     gene            complement(9897..10529)
                     /gene="pyrE"
                     /locus_tag="PKH68_04150"
     CDS             complement(9897..10529)
                     /gene="pyrE"
                     /locus_tag="PKH68_04150"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNX12162.1"
                     /translation="MKTSEKSIAEKLLKVKAVKLQPNNPFVWASGWNSPIYCDNRKTL
                     SYPSIRNYIKVELVRTIINLYPDVEVIAGVATGAIAQGALVADELGLPFIYVRSSPKD
                     HGLENLIEGDLKPKQKVVIIEDLISTGGSSLKAVEAIRKDGSEVLGMVAIFTYGFTIA
                     EDKFKNAKVKLTALSNYDAVLSEALATNYINEKDMETLKEWRQNPSVWKK"
     gene            complement(10680..11045)
                     /locus_tag="PKH68_04155"
     CDS             complement(10680..11045)
                     /locus_tag="PKH68_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNX12163.1"
                     /translation="MADHNDLGNLGEERAQAFLRQKGYDIKETNWVTGKLEIDIIALK
                     DNTLVIVEVKTRSTEFFEHPKEAITYRKIKNLVNAAHNYIMKTNWMGETRFDVISVIP
                     HGQQFVIEHIENAFIPPVN"
     gene            11090..11416
                     /locus_tag="PKH68_04160"
     CDS             11090..11416
                     /locus_tag="PKH68_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12164.1"
                     /translation="MMLLLRSDLTQNYAFSGINTEIQRYNIIFVENILKMAKKKYLKP
                     SPNPVLPRKYRQIFTLNEKENNALNRFLEKYKVKNKSKFIRETLMTEIIRKTEADQPT
                     LFDNIK"
     gene            11430..12779
                     /gene="rmuC"
                     /locus_tag="PKH68_04165"
     CDS             11430..12779
                     /gene="rmuC"
                     /locus_tag="PKH68_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNX12165.1"
                     /translation="MQLSLLLLFVFISLIIGFVAAWLLQSRKVNDLKRGYESTVNQAL
                     EEKNILENQYLVAQERLTLRTEELLKIQGEFQKINNLAENRGMELAGIRTTNANLLEK
                     LENQKREIIEMQNRLTVEFENIATKILKERSDEFSATNHKNLYQILNPLQEKIQLFEK
                     KVEETYDKDLRDKISLKEEIKKLTELNRRVSDEATNLTNALKGDVKKQGNWGEIVLER
                     VLERSGLTKGQEYETQVDITGSDDSRQRPDVIVHLPDDKHIIIDAKVSLIAYERFSSA
                     GTPEEQIRHQKEHINSLRAHVKELSEKNYQEGMRINSPDFVLMFLPIEASFSVAVQAD
                     SELFAYAWDRKIVIVSPTTLLATLRTISSIWRQENQTRNAQEIARLSKALHDKFLAFV
                     DDMSKIKINLDRASGAYDDALKKMKDGKGNIIRTAERIQELGGFDGKKSLPGDFEIF"
     gene            12833..14356
                     /locus_tag="PKH68_04170"
     CDS             12833..14356
                     /locus_tag="PKH68_04170"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNX12166.1"
                     /translation="MKKYVKILSLIVFVNFYAAGVFSAVRYTALTDTLTVVDTTNNPR
                     SSFGDSLRISKPDTTQLLPNNTLLQKNDSVFHAVKPVYKYTLLTPSGAVNVDNSYFVN
                     KDVLNHRLDSLMKLPQKPTDSLLYAASPFHVPLIFMGKNLKPVWDGHIDYQNEFYGKK
                     MSPFEQIQLKSTSSETLVDETRKIARNYISSHALHLYETTLDRLPDPGTFTQKLIKHK
                     AVETFTFEEKGNGVDVKKIEVVRLKPRYWNPKATALMQFTQNYISQNWYQGGNSNLAI
                     LNILSGELNYDNKKDVQWENKMEWRAGFNSVVGDTIRKISTNDDLLRYTSKFGLKASG
                     NWYYSVSGDFSTQLFDTYKAINSTQYKARLLTPFRANVGIGMDYKYKKLLSLMIAPLS
                     FKYIYANDTANVNPNLFGIEKGKNQLRQVGSSFRAQLNYSPSRQWQLDSRLTFYTDYK
                     KVEIDWEMVSNLSVNRFMSVRIALNPRYDNTVILKKGENARLQFKQLLSVGFSYRLL"
     gene            complement(14351..14959)
                     /locus_tag="PKH68_04175"
     CDS             complement(14351..14959)
                     /locus_tag="PKH68_04175"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNX12167.1"
                     /translation="MKQTKLQLLPNYSGHRLECGCDEAGRGCLAGPVVAAAVILPLDF
                     SCPGLNDSKQLTELQRDELRIIIEKEALAWSVALVDEKEIDEINILNASILAMHRAVD
                     KLTVTPEFIIVDGNKFKKYDNIPHQTIVKGDSKYLSIAAASVLAKTHRDELMMKLHGE
                     FPQYGWDRNKAYPTIKHRQAIKQYGVSPYHRLSYKLLSNTDL"
     gene            complement(14946..15491)
                     /locus_tag="PKH68_04180"
     CDS             complement(14946..15491)
                     /locus_tag="PKH68_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNX12168.1"
                     /translation="MNTFDLFIFIPIALGLIFGLFRGFVKEVVSLVAVILAIVAAEML
                     SPAFTGIISGLFSFSDKTAKTAAYVFVFIITIIIALLVSRLADKLISSISLGWLNSLL
                     GGVFGGLKFAIIISVLMNVFDALDTKFHFARPESKTESIGYYPVLKLAPSLWDQSKEA
                     YQKSKNAHQDEDKTTPENETN"
BASE COUNT         4385 a         3313 c         3411 g         4545 t
ORIGIN      
        1 caggccaaaa cagccaatga cattactccg ctggtaaaga aaaaattagg tgtgaaagat
       61 cagcctgctg ctcctgctgc aaaataattc ttcaacataa cacaaaaaaa gaaagctgat
      121 tcggtacttc gaatcagctt tcttttttgt cttttagcag gtgtttcaga taatcgaaag
      181 gatgataaag caacatccgc ggacctgaat accgcattat ttccctaatc cgggctctca
      241 tttcaggctt ataacaatgc actttgcatt ttgagcaggc ttttttgtcg tccccaaagg
      301 gacatttgtc caatcgggcg gaggcgtagt cgttaaccgc ttcacattcg gcacacagac
      361 ctctgtcagt tccgtgcttt cggctgcaat atagcccgat catcttttct acagtctctt
      421 tctcgtattt tatggatgac attttgttct tttttctttt atttcttgtc gataagagct
      481 atcatactct ttagctcttc ccttactgtt gttagcctct ctttaatttc ttgctgtttg
      541 gccactacat cctttttgtc actcagtttt tttcttaccc cttcgagcgt aagtttctga
      601 tcattgacca ggaatacaat ctgcttgatg agttgtaagt catcttttga gtagaaacgt
      661 gttccttttt cgttacgtcg gggtttcagt tgtttgaatt ctttttccca gaaccgaagg
      721 ttagattgat tgatctgaaa catttcagcc acttcggata ttgaataata aattttttcc
      781 atgatgaaag atattaacta tgtcggattt taagcacttg tccggttttt attttgctgt
      841 tgcctaagtt attccatttt tttagattcg acacactcac attatatttc tcagcaatgc
      901 ctcccagtgt ctgaccccgt tttactttgt gcaaagtgct ttgttctttt ttttgttcgg
      961 atgtcttggc ggttgttgta ggtttaacct gctcaatttc ggcacggcgg ttattgatca
     1021 gtacgtcggc ccggtaagcc agtatttcat cctttttgtc aatcatcagt ccggcgtagt
     1081 tttgcggaag acacagcgga taaggtttaa tatcgccggg aataatattt cgccggtact
     1141 gcggattaag caggcgtagt tcctcaacgg gcagtttcag aatggctgaa atttgctcaa
     1201 aatgcatccg ggtattgatc atgattgtat ctgtaagtgc aggtattctc acttttacag
     1261 ggcagaggtt gtgctgggca gcataattga gggtgtaatt ggcggcaata aaaatcggta
     1321 cataagcacg ggtctgaacg ggcaggtaag ggtagatggc ccagaaatcc ggtttaccgc
     1381 cggcacgcct gatggcttta tttatacttc ccggaccaca attataggct gcaatgacca
     1441 ggtgccagtc cgaatagatg gagtaaagtt ctttcaggta ttgcacggct gctttagagg
     1501 aagctaccgg attcatgcgt tcgtccacca gcgtattgac ttccagtccg tacattttac
     1561 cggtgccaat cagaaactgc caaagtcctg tagcacccga acgggaaacc gctgtgggat
     1621 ttaaggcgga ttctatgatt ggcagtattt ttaattccag tggcagttgt tctttttcta
     1681 aggcttcttc gaaaatgggg aaataatgtc ggctcattcc aagcatatat tccacttcgc
     1741 gcctgttttt ccgggtgtaa atttcgataa atgacttaac ctcctcatta aagggcattt
     1801 caatttcatg cggcaatttc gccagtcttt ctcggtactg ctcgtcggtg gttgccggat
     1861 tggtctgtga ccagcaatcc gggggagtgt ctttatcaac cgcccaggaa tgcagcaggt
     1921 ggtccagtga gccggaaaaa tcttcgggaa caattaacgc tgaatcggca ctcaccgcag
     1981 gactaaaccc tgtgtcattc gctttcaggt tatcattttg tgcaactccc cgcaagcata
     2041 ggaacagaaa aaggagtatg tggaaaacat ggcttttagt catatttcag gtattaagtc
     2101 agtttagaaa cattctgccg actattattt cgttagaagc gaacagcaaa cttacataaa
     2161 aatctaagat ttataagcag gtcagttaaa aaagttgatt gagaaaaatt acagatgaaa
     2221 gctgaaaatc aattcaaacc caaaaaataa tatataaaat gtataacaaa gaaaacagga
     2281 ataaaactgg tggaaagtca aaatttaata ctccagtgca ttccgtaagc aggaggcgat
     2341 tggcgttccc gctctatacg ggtaggttga acattgagta ccagatccgg gctgatatca
     2401 aaatcgtaca actgagcatc aacgtaggca tcaagaagta ccagcgcgta atatcccacc
     2461 gacaggataa tgctcaaatc ccgccactgc ctgaaagtac tttggcgggt tttcagtata
     2521 ttggtgtaag tgccttcacc gatcatctga acggtgtatc cgcgtggcag aatatcgatg
     2581 tggctgttgg tggtggggtc gccatcaatg atgtcgcggt acgcattttt ataggtgata
     2641 tagttgctgt tgttccacga aatggcatag gcaaatccaa ggtaaccacc atacacaatg
     2701 ggcagtttcc agtatttatg gttgacaatc tgcccaaatc ccggaatggc agctcccatc
     2761 cagattgaac gtaccggatc gggtttgaat tttggcttct cgagtgagga tacaacaacc
     2821 gtatcggcca taacgggagg catgttcata tatttattgc tgggctgaat atccattggt
     2881 gtcagcgtgg tggtatcgtc aacagccacg gcgatattca aatcgttatt ctcaatgcat
     2941 atattaactc ctgcaaagcc gggaattata aaaaacagaa gaaacagatt tgtaacaagt
     3001 gtttttttta acttattttt tttgaaaatc atattttatc tatcagttgc atgatacgca
     3061 tcaactcatc atcattggcg aaaggaatgg taattttgcc ctttcccttt tggtcgaccg
     3121 acatttggac tttggtgtca aaaatagtac ttaactggct tttcaggtcg tttaattctt
     3181 ttaaatcctt aactatattg gtttttccct gtcccggatt accctcaaat tttccgttgg
     3241 ccagaaaatt tcgtaccatt tcttccactt ttctgacatt gagatccttt ttcagaataa
     3301 gatcatataa cttgagttgt acggccggtt cgctcagtcc caggatagcc cgggcatgac
     3361 ccatgtcgat ttttttattt ttcaagccca tctgtatctc tgccggaagg cgcagcaaac
     3421 gcagataatt ggctattgta gccctttttt tgcctacacg gtcactcagg cggtcctggg
     3481 taagtttgta ttcttccatc agacggtaga aggtaagcgc aatttcaatg gcattgaggt
     3541 cttcgcgttg aatgttttca atcagtgcca tctccatcac ttcctcatcg gcggctgttt
     3601 tcacatacgc cgggatactg gttagcccga tagatttagc tgcccggaag cgccgttcac
     3661 cggctattat cagataggtt tcatcgtcgt ttttacgtag tgtgacagga gaaataactc
     3721 caagttcccg aatggaattg gccagttcgg tgagtgattc ctcgtcaaag tgtgttctcg
     3781 gctggttggg gttaggatat ataagtgcta tttccacttc attaatggat gatgaaccac
     3841 ttgtactgac tgaaccaata tcgattagtg ctcccagtcc acgtccgaga cctgtatttt
     3901 ttgccatatt tattgcttga ttgtgtgtat attattgtcg ggtgatcagc tctttggcca
     3961 gatcctgata atttaccgat ccgcgtgatt cgttgtcgaa cagcgaaatt ggtttgccat
     4021 aactcggtgc ctcactcagt cgtacgtttc ggtaaatgac cgtgtcgaaa accaggttat
     4081 cgaaatgctt tttcacctcc gcaaatacct gattggcaga gcgtaagcgg ttgtcataca
     4141 tggtcatcag gaatccttca atttcaagta tcggattgag tttatttttt attatcttta
     4201 tggtattgag cagtttgctg ataccttcca gggcaaaata ttcactttga accggtataa
     4261 ttaccgaatt ggaagctgtg agtgcattaa cagtaatcag cccgagcgat ggtgagcagt
     4321 caataagaat gaaatcatag tcatttttta tcggtttcag catcttctcc atcacatgtt
     4381 cccgtttctc cagattgaga agctctattt cagcacctac cagatcgatg tgcgagggaa
     4441 gtataaaaag attttccact tcggtttggt gtatagcctg tggagaaggc acattgtcaa
     4501 taaggcactc ataaacagtg aattcaacag tccggacatc gatacccaaa cccgaagatg
     4561 catttgcctg tggatcggca tcaaccaaaa gtaccttttt gccaagagaa gccagtgatg
     4621 cagccagatt aatggctgtt gttgttttac caactcctcc tttttgattg gctatagaaa
     4681 ttatttttcc catataattc gttaaagatt atttttttat tcaacagaaa acaacctgta
     4741 aataggatta acagccacac gtacagggtg ataagcaata agcaatttct tggtttattg
     4801 aatgcaaaga taataaaata aatttagcct tttaactctg taaatgctca atcggtatta
     4861 taagattttt tatcatctga gagtggcccg gtaagttttt ttttgaaaaa tcaatcaggc
     4921 ggtctatgtg cttttttagt tgtccggatt atatttcgga gagtacaaaa ataatagtta
     4981 ctccgaaatt gattatcttt gtcctaatta atgattgtat gaagctgcaa aggtatattt
     5041 tacttctttt cgttgtgtta ctggcatttt tttcctgccg ggaagatttg gttgtgccaa
     5101 ctgtcgatcc accaaaaccg gacaatccca cggtcgatat catcactcat tccgacggtt
     5161 tgcaatatgt ttttgacatc gatgcacttc ctgaaattta ccttgaaatt tctactgctg
     5221 aatggaaccg gttgctctcc aattttgaca tcaaccgcga caacgaagaa tatattcaga
     5281 gcaattgtgt atttgtaaaa aacagcagac gagatacgct caataatgcc ggtctccgac
     5341 tgcgcggaaa caccagtcgc cgcaggcccg aaggaactca ggggcagaaa cacaacagct
     5401 cgaatcccgt ctggcatcac gtacatttta cgctcaattt tgaaaaatac gacaaaaaac
     5461 aggatctgca cggactggaa aaaatcaccc tgaaatggtt caaggacgat ggggtgtatg
     5521 cacgcgaagt ctattgttat gacttgttcg agcgatttgg agtatggaca agtcctaaat
     5581 ccagctactg taaactctat ctgcggatta ccggggataa ccggcaagtg tactatggtg
     5641 tgtatcagat tgtggaacag attgacaaac agtatattaa aaaccgatcc ggaaaatttg
     5701 ccggaaatga aggaaacctc tggaaagcca cctggggagc cgatttcaaa aatgctgata
     5761 aaaacaaaat gggtgttgaa aatattacgc tcaatccggc caccatggta cagccggtat
     5821 atgacctgaa aaccaacgaa gccagtcttg aagcagctaa aacacaactg gaagctttta
     5881 tcaattattt caaccgaaaa acaggggatg atttcaaaac ctggattgcg caacggatgg
     5941 atgtaccgct tttcctgaaa acctatgcgg tgagtacatt gtgcggtatg tgggacgatt
     6001 actggaacaa ttcaaacaat ttttattttt attttgatac ggacgaccgg ttttatttta
     6061 ttccctatga ttatgacaac accctgggca cctccctgct gatggccaat tcaggaactc
     6121 aggatttgct caactggggt cagagcacca atccactggt agctaaaatc attnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagcatt tacccaatta agcgatttgg
     6301 aaaaatgaaa aaaatatcaa aatcaacgta tatcatattt gttgcaattg ccgcattgac
     6361 ggtagtttca tccctgtttt tttaccgcat cgatctcact acagacaagc gctacagcat
     6421 cagtccgcag accaaaacac tcatgcactc ggtgcaggaa cccgtaacgg tagtggttta
     6481 tcttaccgga gatctcaatc ccggttttct cagactgaaa cgggctaccg ttgaactgct
     6541 cgacgaactg aatatttatt ctaaaaagca attcaacatt agttatatca atccgtcgga
     6601 ggcagcaacg agtgaagagc gcgagcaaaa ttatctgcat cttgagcaac gtggcttgac
     6661 tgcagcatcg gtgtatgaaa aagacaagga gggaaaaaat attcaaaaaa ttatttatcc
     6721 ctggctggaa atcagctacc ggggaaaaac cattccggtc aatctgttga aaaatattcc
     6781 gggatattcg ggtgaagaaa acctgaatat ttccatcgaa aatctggaat ttgaactgac
     6841 cgatgctctg cggcggctga taaatacgaa agtggagaaa attgcatttc tcgaaggtca
     6901 tggagagtta agcgaagaag aaacgctgga tataagtcag agtttgagcc gctattatca
     6961 gattgaccgt ggcactatag gcacagatcc tgcggtgttg aacgattata aagcagtgat
     7021 tattgccaaa cccacattgc ctttcagcga aaaggataaa tttgtgatag accagtatat
     7081 catgcgtggc ggcagggtac tatggctggt tgacggagtg cgtgtttcgc ccgaaagtct
     7141 ttcgaccggt gggtttactc cggcggtgga gctggatctg aacctgagcg atatgctctt
     7201 caggtatggt gtccgtatca gcccggtgct ggtggaagat gttcagagtg tattaattcc
     7261 ggttaatgtg gctgccaaag gggagaaacc tcaatttcaa cccacgccct gggtgtatgc
     7321 accgctgttg ctgacatcgg gcgatcatcc tgtcacccga aacctggcac ctgtaaaggc
     7381 aaattttgcc tcggcaatcg agatggtagg ggacgataaa aaactggaac gaacttttct
     7441 gctcgccagt tcggacaata cacatattct tcagacaccc gccgaaattt cactggcagc
     7501 catgcccgac ctcaccgata aaaactattt taaccgttcg tacgtacctg taggagtggt
     7561 tttggaaggc gaatttcagt ctgattttac caaccggatg cctcctgaag agatagtgaa
     7621 cccacccgca attcaaacca aaagtgtccc cggacgccag atagtgatag ccgatggcga
     7681 tataatccgc aacgaaacag taggaacggg aggcaataaa aaggtaattc cgttgggttt
     7741 tgatcggtat atgaaccaac aattcggcaa taaagatctg ataaccaatg ctgtgctgta
     7801 tctgaccgat aaaaatggct ggatggagct tcgttcacga acagtggcac ttcggctgct
     7861 caacaaaaaa atatccgcca tcgaaaaaac gaaatggcag ataattaatg tggtaattcc
     7921 accggttgtt ctactactgg ccggactgct ttatcagtgg atcaggcgca ggcgatatgc
     7981 ccggtaaaca tcagctgttc agcagattct gttctgcttc atgcactcca ctgaaattaa
     8041 actgttcccg gatgctgtcc ttgtaaattt ttatccggtc gttacacaga ttaccgatac
     8101 taccttcgtg ggtatggtgt atgtttttat cggggttaaa atttcccgct tcaatgtcca
     8161 gacccacttt tttgtaggca tcgcggaaag gcattccgtt ttgcgcaagc cggttcactt
     8221 cttccacact gaaaatcaaa tcgtatttgg gatcatcaag tatgtcggtt ttcacatcca
     8281 cccgttccat catcatcgct gccatttcca gacaatcgcg aagctcaccg aatgccggca
     8341 ggaacacttc tttgataatc tgcagatcgc ggaagtaacc cgaaggtaaa ttagttgtaa
     8401 tcagagatat ttgctgtggt atcgactgga gtttgttgca tttggcacgt gtaagttcaa
     8461 atacatcggg attttttttg tggggcatga tgctcgatcc ggttgtgaac tcatcgggca
     8521 gtttgataaa tccaaagttt tgtgaggtaa acatacaagc atcaaaagcc agttttgata
     8581 ccgtggcggc aactccggca agcgccgtgg ctacaatacg ttccatttta ccgcgtccca
     8641 tctgtgcata caccacgttg taattcatgg tgtcaaatcc cagcaaatcg gttgtcatct
     8701 ggcggttgag cgggaaggaa gagccatatc cggctgccga tccgagtgga tttcggttgg
     8761 tgattttcca ggcagcaagg agcatctgca agtcgtcggc caggctctcg gcataagcgc
     8821 caaaccagag cccgaaagac gatggcatgg caatctgcag atgggtgtag ccgggcagca
     8881 gaatatgttt gtatttttcg ctttgctcta tcagtacctc aaaaagcgca gaaacagcat
     8941 cgaccgtttt ttctatttca gcccgggtat aaagtttcaa atctaccagc acctggtcat
     9001 tgcgtgaacg gccgctgtga atttttttgc ccacatcacc cagtttgcgc gtaagcatca
     9061 gttctacctg cgaatgtaca tcttctactc cttcttcaat aataaaatca tcatttacag
     9121 ccagttggta gatgtttttc agttcccgca gcaatacatc aagttcctcc ctgctgagta
     9181 acccgaccga ttgaagcatc agaatatgag ccatggagcc gagaatatca tattttgcca
     9241 gataaatatc cagcatgcgg tcgttaccga cagtgaattt ttcgattttt ttattcacct
     9301 gtgttgactt ctcccatagt ttcatgataa tgtctccttt attttcccga aaacggaaat
     9361 ttttagtaaa aacaaattat tattgaattt aaatacaaaa ttaccggctt aaatttattt
     9421 agccgtcttc atactcagaa actgcaccag tgcatcagca atcatgttaa ttcctttttt
     9481 cagtttactg tcggccatca tttttatcat catgggcata tcggctttca gggtaagttt
     9541 catgcgggtg tcattttcgg ccacttgcac cagttgtatc cagccattta cagctattgg
     9601 agagttttca gcttccagtt tgatggtttt gaagggctcc cgttcggtaa tgcggaaacc
     9661 gactttgccg attccttcca ccgaaaaccg gcacgaatca tcatcaaatt ccacatcctt
     9721 cagcatttcg ttaagttttg cctgagcttc ggcattcatg ttttgtagat tactgaaatt
     9781 cgacagtttg ctcaattgat taaaaaccac ttctccggat gaagaaatgg tttttacgtc
     9841 actttcgtag gtcgtcatct tgtacgttta tgtaaataat atggtttatt cgatgattat
     9901 tttttccaca cggaagggtt ctgacgccat tccttcagag tttccatatc tttttcgttg
     9961 atgtagttgg tagccagggc ttcggaaagt acggcatcgt agttgctcag cgcagtgagt
    10021 tttacttttg catttttgaa tttatcttcg gcaattgtaa aaccgtaagt gaaaattgcc
    10081 accatgccaa gtacttcgga gccgtctttg cggatggctt ctacggcttt gaggctgctg
    10141 ccgccggtgg agatcaggtc ttcaataatt accacttttt gttttggctt taggtcacct
    10201 tctatcagat tttcaagtcc gtgatccttt gggctggagc gtacgtagat gaatggcaat
    10261 ccgagttcgt cggccaccag ggcaccctga gcaatggctc cggttgccac gccggctatc
    10321 acttccacat cggggtagag gttgatgatg gttcgtacaa gttctacttt tatatagttg
    10381 cggatggagg gatacgacaa agttttacga ttgtcgcagt atataggtga attccaaccg
    10441 gaggcccata caaaagggtt attgggttgc aattttacag ctttcacttt caggagtttt
    10501 tctgcaattg atttttcgga agttttcatc gtttaatttc ggttttttgg aaaaaggcca
    10561 taggagcagc ccaaattcat atgtttataa tccgcaaaag tacacaaact atttaataat
    10621 aaaaagttaa atcatgtggt ttggtgaatg tgaaacagga tgttatcacc cgatcagtat
    10681 cagttgaccg gaggtataaa ggcgttctca atgtgctcaa tgacaaattg ctgaccatga
    10741 ggtatcaccg atatcacgtc aaagcgggtt tcacccatcc agttggtttt cataatataa
    10801 ttgtgagcgg cattgaccag atttttaatt tttcggtagg tgatggcttc tttcgggtgt
    10861 tcaaaaaatt cggttgaccg ggttttaacc tctactatga ccaatgtgtt gtccttcaaa
    10921 gctataatgt caatctctaa ctttccggtc acccagtttg tttcttttat gtcataccct
    10981 ttttgacgca gaaaagcctg tgcacgttct tcgcccagat tacccaaatc gttgtggtct
    11041 gccatttata tttaaaaatt taattatttg tgtcttttcc ttcggctgta tgatgctgct
    11101 actcaggtca gacctgacac aaaattacgc tttttccgga attaatactg aaattcagcg
    11161 atataatatt atttttgtgg aaaatattct gaaaatggca aagaaaaaat acttgaaacc
    11221 ttccccaaat cctgttttac cccgaaagta cagacagatt ttcactctca atgaaaagga
    11281 aaacaatgcg ttgaaccggt ttttggaaaa atataaagtt aaaaacaaat caaaattcat
    11341 tcgtgaaacc ctgatgacgg agataatccg taaaacggaa gccgaccaac ccaccttgtt
    11401 tgacaatata aaataaaaaa accccctgca tgcaactatc tttgctgtta ttgtttgtct
    11461 ttatttcgct gattattggt tttgtggctg cctggctgtt acaatcccga aaagtcaacg
    11521 atttgaaaag aggctatgaa tctaccgtca atcaggcgct ggaagagaag aatattctgg
    11581 aaaatcaata ccttgttgca caggaaagac ttactctccg aaccgaagag ttactgaaaa
    11641 ttcagggtga atttcaaaaa attaacaacc tggccgaaaa tcgcggcatg gaacttgcag
    11701 gcatacgtac aacgaatgca aacctgcttg aaaaactgga aaaccagaaa cgggaaatca
    11761 ttgaaatgca aaacaggttg acagtggaat ttgagaacat tgccaccaaa atactgaagg
    11821 aacgttccga tgaattttcg gcaaccaatc acaaaaatct ctatcagatt ttgaacccac
    11881 tgcaggaaaa aatacaactt tttgagaaaa aggtggaaga aacctacgat aaggacctgc
    11941 gcgacaaaat aagtctgaag gaagaaataa aaaaactgac cgagttaaac agacgggtaa
    12001 gtgacgaagc gactaacctt accaatgccc tgaaaggaga tgtgaagaag cagggaaact
    12061 ggggtgaaat agtgctggag cgggtgcttg agcggtcggg actgaccaaa ggacaggaat
    12121 acgaaaccca ggtggacatc accggcagcg atgattcacg gcagcgcccc gatgttattg
    12181 ttcatctgcc cgacgacaag catataataa ttgatgccaa agtatcgtta attgcttacg
    12241 agcggttttc gtcggccggg acaccggaag agcaaatcag gcatcaaaaa gagcatatca
    12301 actcacttcg ggcacatgta aaggaattga gtgagaagaa ttatcaggaa gggatgagga
    12361 tcaattcacc cgattttgtg ttgatgtttc tgcccattga ggcttctttt tcggtggccg
    12421 tgcaggctga tagcgaactc tttgcttacg cctgggacag aaaaatcgtg attgtaagcc
    12481 caactacgct gcttgccaca ctcaggacca tttcttctat ctggcgtcag gaaaatcaaa
    12541 cccgaaatgc ccaggaaata gcccgactga gcaaagcgct gcacgataaa tttcttgctt
    12601 ttgtggatga tatgtcaaaa ataaaaatca acctggatcg ggcttcgggg gcgtatgacg
    12661 atgcgctgaa gaaaatgaaa gacggtaagg gaaatataat tcgtaccgca gagcgcattc
    12721 aggaactggg cggattcgac gggaaaaaat cacttcccgg tgattttgaa attttttaat
    12781 tacatccaca ttcacacccg ggtttattgg tggatgaaaa aaagaatatt gtatgaaaaa
    12841 gtatgtgaag attttaagtt tgattgtttt tgttaatttt tatgctgccg gagtgttttc
    12901 ggcagtgagg tatacagctc tcaccgatac gcttacagta gttgatacta cgaataatcc
    12961 acgatcatcc tttggtgatt cgctaagaat atcgaagccg gataccacac agttgttgcc
    13021 caataatacg cttttgcaga agaatgacag tgtctttcat gctgtgaaac ctgtttataa
    13081 atacacacta ttgacacctt cgggagccgt aaatgttgac aattcttatt ttgtgaataa
    13141 ggatgttcta aatcaccggc ttgattcgct gatgaaactg cctcaaaaac ccaccgattc
    13201 cttattgtat gccgccagcc cattccatgt gcctcttatt tttatgggaa aaaacctgaa
    13261 gccggtgtgg gacggtcata ttgattatca gaatgagttt tacggaaaaa aaatgtctcc
    13321 atttgaacag attcagctca aaagtacatc atccgaaact ctggttgacg aaacacgcaa
    13381 aattgcgcgt aattatattt ccagtcatgc gcttcatcta tacgaaacta ccctcgaccg
    13441 cttacccgat ccgggaactt tcacccagaa actgatcaaa cacaaagcgg tagaaacatt
    13501 tacattcgaa gaaaaaggca acggggtaga tgtgaagaaa attgaggtag tcagactcaa
    13561 acccaggtac tggaacccca aagcaactgc tttaatgcag tttacacaaa attatatttc
    13621 acaaaactgg tatcagggcg gtaattccaa cctggctata ctcaatatcc tgagtggcga
    13681 attgaattac gataataaaa aagacgttca gtgggagaat aaaatggaat ggcgtgccgg
    13741 gttcaattcg gtggtgggcg ataccatccg gaaaatcagt accaatgatg atttgctccg
    13801 atatacttcc aaattcggcc tcaaagcaag cggaaactgg tattacagcg tctcgggaga
    13861 tttttctacg cagttattcg acacttataa agccattaac tctacccagt acaaggcacg
    13921 gcttctcaca ccattcagag ctaatgtcgg catcgggatg gactacaagt ataaaaaatt
    13981 gctgtcattg atgatagcgc cactttcatt caaatacatt tatgccaacg atacggccaa
    14041 tgtaaatccc aacttatttg gtattgaaaa aggtaaaaac cagttgcggc aggtgggtag
    14101 ttcgtttcgt gctcagctga attacagccc atcgcgtcag tggcagctgg attcccgcct
    14161 gaccttttac accgattaca aaaaagtgga aattgactgg gagatggtaa gtaatctgtc
    14221 ggtcaaccgg tttatgtccg ttcgtattgc cctcaatccg cgatacgaca ataccgttat
    14281 cctcaaaaag ggtgaaaatg cccgtctgca gttcaaacag cttctcagtg ttggtttctc
    14341 gtatcggttg ttataaatcc gtattgctca ggagcttgta gctaagccgg tgatatgggc
    14401 tcactccgta ttgctttatt gcctgacggt gtttgatagt ggggtaagct ttgtttcgat
    14461 cccagccgta ttgaggaaat tccccgtgta atttcatcat cagttcgtcc cggtgagttt
    14521 ttgccagtac cgatgcagcc gctatcgaca ggtacttgct gtcacctttc acaatggtct
    14581 gatgaggtat gttatcgtac tttttaaatt tatttccatc cacaataata aattcgggtg
    14641 tcaccgtcag tttatccaca gccctgtgca ttgccagtat ggaagcattc agaatgttga
    14701 tttcatcaat ttctttttca tcgaccagtg ccacactcca ggcaagtgcc tccttttcaa
    14761 taataatccg gagttcgtcc cgctgaagtt cggttaattg tttcgaatcg ttcagcccgg
    14821 ggcaactgaa gtcaagaggt aaaatgaccg ctgccgctac cacagggcct gccagacatc
    14881 cgcgaccggc ttcatcgcaa ccacattcga gtctgtgccc tgagtaattt ggaagaagtt
    14941 ggagtttagt ttgtttcatt ttcgggtgta gtcttatctt cgtcctgatg ggcgtttttg
    15001 cttttttgat aggcttcttt cgactgatcc cagagcgagg gtgccagttt aagtacggga
    15061 tagtagccga tagattctgt tttggattcg gggcgggcaa aatgaaattt ggtgtcgagt
    15121 gcatcaaata cattcatcag tacactgatg ataatggcaa atttcaatcc tccgaaaacg
    15181 cctcccagca gcgaattgag ccatcccagg ctgatgctcg aaatcagttt gtcggccaga
    15241 cgcgacacca gcagagctat aatgatagta atgataaaaa caaaaacata agcagccgtt
    15301 ttggctgttt tgtcggaaaa actgaataaa ccggaaataa tgcctgtgaa cgccggactt
    15361 aacatttcgg cagcaacgat ggcaagtatt acggcaacca gtgatacaac ttctttgaca
    15421 aagcccctga aaagcccgaa aataagacca agcgctatgg ggataaaaat aaacaaatcg
    15481 aacgtattca taaatttcga attttattaa tggctacaaa tatcgtgaaa aatctctaaa
    15541 cataaaaagc atcccgggaa aggatgcttt ttcagaaaaa aaatatctac aatcttaatt
    15601 atttaaccaa tactttgtga gcagtgccgt tgatgcgaag aatgtatgtt cctgcactta
    15661 ccgactgaga aaattcattt gcagctttgg cgttgcgtat caactgaccc gacaaagtga
    15721 aaagttctac ctgagcttcg tcgttgaaag tggc
//
LOCUS       DAOPZK010000043        15362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_4150_length_15362_cov_23.332005, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000043 DAOPZK010000000
VERSION     DAOPZK010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15362
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4150_length_15362_cov_23.332005"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKH68_04185"
     CDS             complement(<1..623)
                     /locus_tag="PKH68_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX12169.1"
                     /translation="MSKTISFNPLTLPTYVMAKPAGSICNLNCSYCYYLEKEKLYQNR
                     KKLEMSDELLELFTKSYIEAQPVKEVLFTWHGGETLLRDISFYRKALLYQRKYGRGRV
                     ITNTLQTNGILLTDEWCRFFKDNNFLIGISIDGPEHYHDAYRKNKGGAGTFRQVMRGI
                     ELLQKHGVDFNTLSVINDYNVQFPLDVYRFFKEIGSQFMQFAPIVERV"
     gene            complement(770..1207)
                     /locus_tag="PKH68_04190"
     CDS             complement(770..1207)
                     /locus_tag="PKH68_04190"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNX12170.1"
                     /translation="MKKLFISFVLLAFAGAVAFAQTDKITGKWKTIDDKDGSEKSIVH
                     IYKATNGKYYGKIEKLFKDPQKLCTECDGANKDKPVMGMVIVNNMVEKDGSLTGGTIL
                     DPKDGKVYQCNISYNAKTGNLKVRGSLDKRGIIGRSQQWIKVN"
     gene            complement(1329..1805)
                     /gene="ybaK"
                     /locus_tag="PKH68_04195"
     CDS             complement(1329..1805)
                     /gene="ybaK"
                     /locus_tag="PKH68_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNX12171.1"
                     /translation="MNKIQKTNVARLLDKAAIAYQLIAYEVDENDLSAVHIADQLNEN
                     PQQIFKTLVLQGNKTGYFVCVIPADEELDLKKAAVVSGNKNCDLIPMKDLLPVTGYIR
                     GACSPMGMKKKFPTYIHNSCTSFSHIFVSAGQRGLQIKIDPFDLIKITRAIPTQLL"
     gene            complement(1900..2259)
                     /locus_tag="PKH68_04200"
     CDS             complement(1900..2259)
                     /locus_tag="PKH68_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HNX12172.1"
                     /translation="MITVLCYPACGTCRKAEKWLKDHKIEYAYRPIKEENPSYEELRL
                     WLDKSGLPVAKFFNTSGLLYKQLNMKEKVKKLPQDELLQLLASDGLLVKRPVLVNGDQ
                     VLVGFKEEEWKEMLNVI"
     gene            complement(2276..3760)
                     /gene="rseP"
                     /locus_tag="PKH68_04205"
     CDS             complement(2276..3760)
                     /gene="rseP"
                     /locus_tag="PKH68_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNX12173.1"
                     /translation="METFLIRALQLILSLSILVVLHEFGHFAFSRLFKVRVEKFYMFF
                     NPKFSLVRAKKINGKWNVKFFAANVPANERMKLDSNGQPMMDGKNPVMEPVPLSEMSQ
                     GDWRRYPESTEWGIGWVPLGGYCKISGMIDESMDKTQMAQPAKLWEYRSRSVWQRLPI
                     IIGGVLVNFILALVIYSAVLFTWGKEYIPLENAKYGLSFSKTMTDIGFQNGDKILTID
                     GEKVDTRADAVEKILIDDAKTVTVERKNQIVRINIPESLTQKILAAGESNLIDIRYPF
                     VVGEVSHGSPAEKAKLIAGDSIIAVNGKEMNIFQDISAELSASKNSLITVTYVRNGIT
                     ENTEVQINEDGKLGVYMKPPSSYIQSEKQEYGFFQSIPAGINMGVETLSSYIKQFKLV
                     FTKEGAKSLGGFGSIGQLFPGTWDWQSFWLMTAFLSVILAFMNFLPIPGLDGGYVLFL
                     VYEMITGRKPSDKFLEYAQTVGMVLLLLLLIYANGNDIFKAFFK"
     gene            complement(3808..4977)
                     /locus_tag="PKH68_04210"
     CDS             complement(3808..4977)
                     /locus_tag="PKH68_04210"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNX12174.1"
                     /translation="MSDKKKNIAILGSTGSIGRQALDVIRANPDKFEVFALTANNNLD
                     LLIEQACEFQPDVVAIANEKLYLQLKQALADQPIKVFAGMDAIAQLVEMEPIDLVLTA
                     MVGYAGLKPTIQAIRANKTIALANKETLVVAGEIIGELALKHKTAILPVDSEHSAIFQ
                     CLAGEGNNPIEKIILTASGGPFRAKTIEELKTVTSAQALKHPNWDMGAKITIDSASMM
                     NKGFEVIEARWLFGLTPDQIEVVVHPQSIIHSMVQFTDGSVKAQLGLPDMKLPIQYAF
                     TYPDRIKTDFPRLDFGRYNELTFEKPDTGRFRNLALAYRAMEKGGNMPCIINAANEIV
                     VAAFLKDKTGFLQMSDIIETVMQKATFIHKPNYEDYVMTDAQVRELTLKEINRQA"
     gene            complement(5005..5874)
                     /locus_tag="PKH68_04215"
     CDS             complement(5005..5874)
                     /locus_tag="PKH68_04215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX12175.1"
                     /translation="MKKELKHILSDFMQLMRFKYRVSILNENTLEENWHTRLSRFSVL
                     LFASAFFLTTFILLTFLIFVTPLRHYLPGYGESGNRIAIVQQSMTTDSLTHQLELQQA
                     YLSVLKDIINEKPQNDTIKSLDSVMLKERATVLLQKSKRENDFIRDYEESQKYNISSI
                     SSASSEKMYVFFRPVRGVIASTYEPQEGNFGISLITSSQENVMSVLEGTVVYAAFTFD
                     YGWVIQVQHENDYISIYKNNTRLLKGAGEHVKAGESIAITGDDQNSGGRKHFYFEIWK
                     QGKPVNPQDVIVF"
     gene            6248..6763
                     /locus_tag="PKH68_04220"
     CDS             6248..6763
                     /locus_tag="PKH68_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNX12176.1"
                     /translation="MKKIGLLTDTHGLLDERIYKYFDEVDEIWHAGDWGSMEVVNKLQ
                     TFKPVRGVWGNIDGQDIRMVFPKHNRFRCEEVDVWLTHIGGYPGKYDAGVLSEISKNP
                     PKLFVCGHSHILKVVYDPKLELLHINPGSAGKYGFHKVQTLIRLVIDGNKIKDLEVIE
                     LKPKAPQPPKE"
     gene            complement(6940..8478)
                     /locus_tag="PKH68_04225"
     CDS             complement(6940..8478)
                     /locus_tag="PKH68_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNX12177.1"
                     /translation="MAENIKQSHPKGLYFVFATGMSERFSYYGMRAIFVLYLIKALLM
                     DKEFASAIYGNYTGLVYLTPLVGGYVADRYLGMRRSIFWGAVLMVLGQLLMFFSALNY
                     QSTELAKWLMYGGLAGLIFGNGFFKPNISTIVGQLYERGDKRLDSAYTIFYMGVNVGA
                     FMAPLLCGYLGDTGDPADFKWGFMAAAIMMGLSLILYVTQKNKYLIGPDGNAIGIYAP
                     HKINKAKDTAEGLPKVKINTNRLLVLGAVAVGLFAVIMHFFDNDVIGAVIYTACIVVP
                     AYVITDSSLSKIEKQRIWVIFIIAFFVIFFWSAFEQAGASLTYFADEQTDRSILGWEM
                     PASWFQSFNPIFVVAFAPLFSMMWLRLGRKNMEPASPTKQAIGLFLLSLGYLLIAFGV
                     KGAAPGAKVSMLWLTGLYFIHSMGELALSPIGLSMVNKLSPVRFASLMMGVWYLSMAT
                     ANKFAGVLSGLYPEAGKPKTFLTFQITDLYDFFMVFVVMSAIASLILFALSKTLQKLM
                     HGVR"
     gene            complement(8612..>8901)
                     /gene="folB"
                     /locus_tag="PKH68_04230"
     CDS             complement(8612..>8901)
                     /gene="folB"
                     /locus_tag="PKH68_04230"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNX12178.1"
                     /translation="EGNTFLVSVIMELDTTLAGKTDNLEDTLNYQQVYDVVRREMEIP
                     SNLIEHAGQRILDAVFDTFTPIKTLKVQLSKLNPPLGGSVESVKIELDKSR"
     assembly_gap    8902..9001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9208..9588)
                     /locus_tag="PKH68_04235"
     CDS             complement(9208..9588)
                     /locus_tag="PKH68_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNX12179.1"
                     /translation="MGLFSALLGNAGTIDPVKLEKEFNNLLTENENIEMGFRLIRDTF
                     IFTNKRMIMINVQGITGSKQEYLSVPYKSISRFCIESAGTFDLEAELKIWISSEQQPS
                     IRKNFTRSVNVYEVQKVLAHYVLK"
     gene            complement(9635..10444)
                     /locus_tag="PKH68_04240"
     CDS             complement(9635..10444)
                     /locus_tag="PKH68_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX12180.1"
                     /translation="MKTVNSMWLSDFGVYYERIEYPQNRNEPVLIFLHDSWGCVEMWG
                     DFPKKMVEITGMNAFLYDRRGYGKSSPFDSTQRNKYYLHDEADELIRIMDRMNIQKAV
                     LYGHSDGATIALIAAANYPDRFDAVILEGAHSFIEEKGKAAVRENRDRARHNSLLQSL
                     RKYHGDKTQELFKRWHETWLSDFFADWSIIPLLTDISCPVLAFRGEKDPYDTEEQLNV
                     LKKEITAPVTIAIIPEAAHTPRVENKTVTMGLIKNFTESIFATGRQDNIEI"
     gene            10590..11996
                     /gene="dacB"
                     /locus_tag="PKH68_04245"
     CDS             10590..11996
                     /gene="dacB"
                     /locus_tag="PKH68_04245"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNX12181.1"
                     /translation="MKKKLSTVFLTFIVCTIFAANPVKSFTDNDLLRKANISITVKDL
                     NTGKIVESYRPECATVPASTLKLVTTATALELLGPEFRFSTTLETDGLITADSTLNGN
                     LYIHGSGDPTLGSAMQGDTAFLTNWKSGILKAGIKRINGSIVADATRFDTQGINPKWL
                     WEDMGNYYGAGAYGISYKDNLFTLEFRSGKAGSTPEIVKMIPEIPDFKFENNLKSSAI
                     GFDSAYFYGAPFSNFRMIYGEIPANRPSFKVKGDIPRPGLLLARDFTAKLQSAGIQVS
                     GIPTDVLTGNNSRSIIYTHFSPPLREIIREINVQSNNHYAEHVFRYLSMVNDTVATSR
                     GSVSVIRKLWASKSLPVDELFMYDGSGLSPVNAVSSGFMAGLLEYMDKSPNKEVFKAS
                     LPVAGTTGTLKSFLKNTPLQGKVRAKSGSIHRVKCYAGYINTKGKNYAFSIMVNNPNG
                     TLSETTKKMEEFLLTITK"
     gene            11993..12724
                     /locus_tag="PKH68_04250"
     CDS             11993..12724
                     /locus_tag="PKH68_04250"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNX12182.1"
                     /translation="MTPTRYYTASTASTNTLLHEMMRQDKLPEGFLLYTDFQSAGKGQ
                     PGNTWESENGKNLLFSMLLYPHSIKVYEQFILSEITGLAIRKVLEKYTDDICIKWPND
                     IYWKDKKIAGILIENSLFRDRIDTCIIGIGLNVNQEVFISNAPNPVSLRQITGNDVNR
                     EILLKEIQAELLNIYQNYSPEFIHQEYLNHLYRRNGFHRYAETAANTVFDAEVEDVLP
                     DGRLILRTQAGEIKSYYFKEIQFVI"
     assembly_gap    12858..12957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12958..13769)
                     /locus_tag="PKH68_04255"
     CDS             complement(<12958..13769)
                     /locus_tag="PKH68_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNX12183.1"
                     /translation="MEEIKQYTPKDFKSDQYVRWCPGCGDYAILNSLQKVMADMGVAP
                     QDIAVISGIGCSSRLPYYINSYGFHTIHGRAAAVASGVKIANPKLTVWQISGDGDGLA
                     IGGNHFIHSVRRNIDMNVLLFNNQIYGLTKGQFSPTTKKGSITKSSPFGTIERPFRPA
                     ELTFGSRGTFFARLLDVDLKNATETIGAAARHKGTSIVECLVNCVIFNHGAHGWIAEK
                     ETRPDRIIFLEHGKPMIYGVNRDKGLVLDGFNLKAVTIGENGITEKDLLVHDA"
     gene            complement(13779..>15362)
                     /locus_tag="PKH68_04260"
     CDS             complement(13779..>15362)
                     /locus_tag="PKH68_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNX12184.1"
                     /translation="PAALKVNAKHVKKGGVIIYDENCFEKSDLVKAGFVTDDPFEELS
                     ISDTISLVPAQLTTLTQKSLEGYPLDNKAILRSKNMFALGLICWLFNRPMDQAIHFLG
                     NKFGKKPSVLEANVKVLTDGFNYGNNLHLTVPTYTVEKSDNVPHGKYTSISGNTATAY
                     GFVAAAEKAGLELFLGSYPITPATDIMHELALRRDLGAKVVQCEDEIAGITTSIGAAF
                     AGCLAVTNTSGPGLALKSEGINLAVMAELPIVIVDVMRSGPSTGMPTKTEQTDLLQAL
                     YGRNGESPLVVMAPGTPDDCFHFAYMASKIALEYMTPVILLTDAFLGNGTSLWKLPKL
                     ADMPEIKPNYVQPGMEGCKVTYRNEDTKVRYWAIPGTPGFEHRNGGLEKDYEKGSIST
                     DGENHQKMVDTRAEKVENVTAVIPDLTVSGSEDADLLVIGWGGTRGHLMSAVSEMNEK
                     GKKVALAHFNYINPLPKNTAEVISKYKKVVVCELNNGQFASFLRSKVPGVNFLQHNKV
                     QGQPFTMAELVEHFELILK"
BASE COUNT         4235 a         3384 c         3116 g         4427 t
ORIGIN      
        1 acacgttcca cgatgggtgc aaactgcata aactgactgc ctatttcctt aaaaaaacga
       61 taaacatcca gtggaaattg cacattgtaa tcgttaataa ccgatagtgt attgaaatca
      121 accccgtgtt tttgcaacag ttcaatgcca cgcattacct gccggaatgt tccggctcct
      181 cccttatttt tccggtacgc atcgtggtaa tgttccggcc cgtcgataga aatgcctatc
      241 aggaaattat tgtctttgaa aaaccggcac cattcatcag tgagaagtat tccgttggtt
      301 tgcaacgtgt tggtaatcac cctgccgcgt ccgtattttc gctgatacag cagtgctttc
      361 cgataaaatg aaatatcgcg caataaggtt tcgccgccgt gccaggtaaa tagcacttct
      421 ttcacaggtt gcgcttcaat gtagcttttt gtaaaaagct cgagcagttc atcactcatt
      481 tccagctttt tacgattttg atacaacttt tccttttcca aataataaca atagctgcaa
      541 ttcaggttac agatggaacc tgcgggctta gccatcacat aggtaggcag tgtaagcgga
      601 ttgaatgaaa tggtcttcga catagtttat cgatttggaa atatgaagag taaaggtaag
      661 ccatttttta gaatcaagag agcaagaatc aagaaaatca gatgaaacct gcgttttttc
      721 atctgatttt caagtcccgt attaacgggc aagtatcaaa gaaatccgtt taattgactt
      781 tgatccattg ctgggaacgg ccaataattc ctcttttgtc gagcgagcca cgcactttga
      841 gattaccggt tttggcattg taactgatat tgcactgata cactttgccg tctttggggt
      901 ccagaatggt gcctccggtc agactgccgt ctttttcaac catattattt acaatcacca
      961 ttcccatgac cggtttatct ttatttgcac cgtcgcattc ggtacataac ttttggggat
     1021 ccttgaataa tttttcgatt tttccgtaat atttcccgtt ggtagcttta taaatatgta
     1081 caatggattt ttcacttcca tccttatcgt cgatggtttt ccattttccg gtaattttat
     1141 ctgtctgtgc aaatgccaca gctcctgcaa aggcaagtaa aacgaatgag atgaacagtt
     1201 ttttcatttt ttatatcgtt aatgttagta ctaaaaaaat tatagctgca aagatagacc
     1261 atatttgcac atttcaactc cgtttgtgca gaaaataaga ttttttagga attacattga
     1321 aaaacaactt acaaaagttg agtgggaatg gcacgtgtga ttttgataag gtcgaaggga
     1381 tcgattttga tttgtaatcc gcgctggccg gcactgacga aaatatgtga gaatgaagta
     1441 cagctattgt gaatgtatgt gggaaatttt tttttcattc ccattgggga acaggccccc
     1501 cggatatatc ctgtaacggg tagtaaatcc ttcatcggaa taagatcaca gtttttatta
     1561 cccgaaacga cggcggcctt tttcagatcg agttcttcgt cagccggaat gacgcagaca
     1621 aagtagccgg ttttattgcc ctgcaacacc agtgttttga aaatctgctg cggattttca
     1681 ttcagttgat ctgctatgtg caccgccgac agatcatttt catccacttc gtaagcaatg
     1741 agttggtaag ctattgctgc tttatccaga agtcgggcta cgttggtttt ctgaatttta
     1801 ttcatttaat tttcctcctg aaataaaaac acaaaaaggc tgattgtaaa taaaatcagc
     1861 ctctttgtta ttgttcgtta tctttttttg aattctaaat caaattacat ttaacatttc
     1921 cttccattcc tcttctttga atcctacaag cacctggtcg ccgtttacca aaaccggtct
     1981 tttcaccagc aagccatcgg atgccagcag ttgaagcagt tcatcctgag gcagtttttt
     2041 tactttttct ttcatattca gttgtttgta aagtaatcca ctggtattga agaattttgc
     2101 aacaggtaat ccggacttat caagccacaa ccggagttcc tcatagcttg gattttcctc
     2161 ctttatcggg cggtaagcat attctatttt atgatccttc agccattttt cggctttgcg
     2221 gcaggtgcca caggcagggt aacacaacac tgtaatcata tttgcagatt gatttttatt
     2281 tgaaaaatgc tttgaaaata tcgttaccgt tggcataaat gagtagcagc agcaacagaa
     2341 ccatgcctac tgtctgtgcg tattccagaa atttatcact gggtttgcgt ccggttatca
     2401 tttcatacac cagaaacaat acatacccac cgtcgagccc cggaatgggc aaaaaattca
     2461 taaatgcaag tattaccgac agaaatgcgg tcatcaacca gaacgactgc cagtcccacg
     2521 tacccgggaa gagctgtccg atagaaccga aaccgccgag cgacttggct ccttctttgg
     2581 taaataccag tttgaattgc ttgatatagc tcgatagcgt ttctactccc atgtttattc
     2641 cggccggtat tgactggaaa aagccgtatt cctgtttttc ggattgaatg taactggagg
     2701 gtggtttcat gtacacaccc agtttaccgt cttcgttgat ctgaacttcc gtattttcgg
     2761 tgatgccgtt gcgcacataa gttaccgtga tcagggaatt tttggaggca ctcagttcgg
     2821 ccgatatatc ctgaaaaata ttcatttcct tgccgttaac ggcaattata ctatcacccg
     2881 cgattaactt tgccttttct gcaggcgatc cgtgtgaaac ttcgcccacc acaaacgggt
     2941 aacgaatatc aatcagatta gattctccgg cagccagaat tttctgagtc aggctttcag
     3001 gaatattgat tcgtactatt tgatttttac gctctacggt gacggttttg gcatcatcga
     3061 taagtatttt ttctacggca tcggcgcgcg tgtctacttt ttcaccgtcg atagtcagaa
     3121 ttttatcacc attctgaaag ccgatatccg tcattgtttt ggaaaaactc aatccgtatt
     3181 ttgcattttc gagcggaata tattcttttc cccaggtaaa taatacggcc gaataaatca
     3241 ccagcgccag aataaaattg acgagtacac ctccgataat gataggcagc cgctgccata
     3301 ccgaacgcga gcggtattcc cacagtttgg ccggttgagc catttgggtt ttgtccatcg
     3361 actcgtcgat catgcctgaa attttgcaat agcctccgag tggcacccac ccaattcccc
     3421 attcggtaga ttcgggataa cggcgccagt caccctgcga catttcgctg agcggaacgg
     3481 gttccattac cggatttttt ccgtccatca tgggctgtcc gttgctgtcc agcttcatcc
     3541 gttcattggc gggtacattg gctgcaaaaa atttcacatt ccatttgcca tttatttttt
     3601 ttgctcttac caatgaaaat ttcggattga aaaacatgta aaatttttct accctgactt
     3661 tgaataagcg ggaaaaagcg aaatgtccaa actcatgcaa tacaaccagt attgataaac
     3721 tcaaaatcaa ttgtaatgct ctgatcaaaa atgtttccat aaattcgttt tacttatttt
     3781 ttcgttttaa atatccgtga tgaagtttca ggcctgcctg tttatttctt tcaacgttaa
     3841 ttcacgtacc tgtgcatcgg tcatcacata gtcttcataa ttgggtttat gtataaatgt
     3901 agccttctgc atcaccgttt caatgatgtc gctcatctgc agaaagccgg ttttatcttt
     3961 caggaatgcc gccactacta tttcgttggc agcattaatg atacaaggca tattgccgcc
     4021 tttttccatg gcccggtaag ccagtgcgag gtttcggaag cgcccggtat cgggtttttc
     4081 aaaagtcagt tcgttgtacc gcccgaaatc cagtcttgga aaatctgttt taatcctgtc
     4141 gggataagtg aatgcatatt gtataggcaa cttcatatcg ggcaatccaa gttgtgcttt
     4201 caccgaacca tcggtaaact gcaccatcga gtggatgata gattgtggat gcaccaccac
     4261 ctcaatctga tcgggtgtaa gtccaaagag ccaacgggct tcgataactt cgaaaccttt
     4321 attcatcata cttgccgagt cgatggttat tttggcaccc atatcccagt tgggatgttt
     4381 gagagcttgt gccgaagtga ctgttttaag ctcttcaatt gttttggcac gaaatggtcc
     4441 gcctgaagct gtaagaatta ttttttctat cggattgtta ccttcgcccg ctaaacactg
     4501 aaagatggct gaatgttcag agtccacggg taaaatagcc gttttgtgct tcagagccaa
     4561 ctcgccgata atttcaccgg ctaccaccag cgtttccttg ttggccagag caattgtttt
     4621 attcgcccgg atagcctgaa tggtgggttt tagtccggca taaccgacca tggcggtaag
     4681 caccaggtca ataggttcca tttccacaag ctgagcgata gcatccattc ccgcaaacac
     4741 tttgatcggt tgatcggcca gtgcctgttt gagctgcaag taaagctttt cattggctat
     4801 tgccaccaca tcgggctgaa actcacaagc ctgctcaatg agtaaatcca ggttgttatt
     4861 agccgtaagt gcaaatacct caaacttatc gggattagcc ctgatcacgt ccagtgcctg
     4921 ccttccgata gaacccgtgg agcccagaat agcaatattt tttttcttgt ctgacatatt
     4981 tgtacttttc atattttcaa cacattaaaa cacaatcaca tcctgaggat tgaccggttt
     5041 tccctgtttc cagatttcga aataaaaatg ttttcgcccg cctgaatttt gatcatcacc
     5101 cgttatggct atactttcac cggcttttac atgttcaccg gcacctttga gtaaccgggt
     5161 attattttta taaatggaaa tgtaatcatt ttcgtgctgc acctgtatca cccacccata
     5221 atcaaatgta aatgcagcat aaactaccgt accttcaagt acactcatca cattttcctg
     5281 cgatgatgta atgagcgaaa tgccgaaatt tccctcctga ggttcgtagg tagaagctat
     5341 cacaccgcgt accggccgaa agaaaacata cattttctca ctggatgccg aactgatgga
     5401 gctgatgttg tacttctgac tttcctcata gtcccttata aaatcgtttt cccgttttga
     5461 cttttgaagc agcacggttg cacgttcttt gagcatgacc gaatcgagcg attttatggt
     5521 atcgttttgc ggtttttcgt tgataatatc cttgagtaca ctcaggtatg cctgttgcag
     5581 ttccagttgg tgtgtaagcg aatcagtcgt catcgattgc tgcacgatag ctatacggtt
     5641 gcccgactct ccataaccgg gcagataatg ccgtaaaggc gtaacaaaaa tcagaaacgt
     5701 gagcagaata aaagttgtga gaaaaaatgc agaggcaaaa agaagtacgc tgaaccgcga
     5761 caatcgggtg tgccagtttt cttccagtgt attttcattg agaatggaaa cacggtactt
     5821 gaatcgcatc agctgcatga aatctgacag gatatgtttg agctctttct tcacacttat
     5881 tgtatctgta aaaaggattg caaatgtagc tctttttatg aaaacgagca aaatataacc
     5941 ctttaagtat cattttaata gttaataatt ccaaacaaaa taccggtcaa aatcattgaa
     6001 tttcaaacat ttatagctgc tgtacaagga aagcacttca tactttcaga cccaatcggg
     6061 tcttaccggc cgtaaaaatt cttacttttg taaaacaaaa aaaacagtca tgaaacaccc
     6121 atgactaaac gataatttag ccaaacaaag gattgattaa accaaatact attcgcacaa
     6181 atctggtata acactcaaaa tccccccctt tagggggtta gggggcagat ataaaaatta
     6241 tacagtcatg aaaaagatag gattactcac cgatacccac gggcttctgg acgaacggat
     6301 ttataaatat ttcgatgaag tggatgaaat ctggcatgca ggcgactggg gttcgatgga
     6361 ggtggtaaac aaacttcaga ctttcaagcc tgtacgcgga gtatggggaa atatcgacgg
     6421 acaggatatc cggatggttt ttccgaaaca taaccggttt cgttgcgaag aagtggatgt
     6481 atggctcacc cacataggcg gatatccggg caaatacgat gccggtgtat tatccgaaat
     6541 cagcaaaaat cctcccaaac tctttgtatg cggacattca cacattctga aagtggtgta
     6601 cgatccgaag ctggaattgc ttcacatcaa tcccggatca gccgggaaat acggttttca
     6661 caaggttcaa acgctcatcc gcctggtgat tgacggaaat aaaatcaaag acctggaagt
     6721 gatagagctt aaaccaaagg ccccccaacc ccctaaagag tgagcttcag aaaatatact
     6781 gagaattttt tattaatatc aacaagtttt tttataacta ttgcaagctg catctttctt
     6841 taagcattcc tttatacgcc gacaatatat tacaaggagt aattacattg aattacaatg
     6901 tcttacatta acttagcaaa ttaataaagt gcggtttttt taccgcactc cgtgcatcag
     6961 tttttgcagt gttttggaga gtgcaaaaag gataagcgac gcaatagccg acatgaccac
     7021 aaacaccata aagaaatcgt agagatcggt aatctggaag gtgaggaaag ttttaggctt
     7081 tcctgcttcg ggataaagtc cactgagcac accggcaaat ttattggcag tagccatcga
     7141 aagataccac acacccatca tcagtgatgc aaatcgtacg ggcgacagtt tattcaccat
     7201 cgaaagtccg atgggcgaca gcgccagttc acccatggaa tgaatgaaat aaagacctgt
     7261 gagccacagc atacttactt tggcacccgg agcagctcct ttgaccccaa aagcgataag
     7321 taaatacccc agagaaagta aaaacagccc gatagcctgc ttggttggcg atgcaggttc
     7381 catgtttttg cggcctaacc gcaaccacat catggagaat agcggagcaa atgcaactac
     7441 aaagattgga ttaaaagact gaaaccagct ggctggcatt tcccagccga gtatacttct
     7501 gtcggtttgc tcatcggcaa aataggtaag tgatgcaccg gcctgctcaa aggccgacca
     7561 gaagaagatg acaaaaaatg caatgataaa tataacccaa atacgctgct tttcgatttt
     7621 cgaaagcgaa ctatcggtga ttacgtaagc cggcactaca atacaagctg tatatatcac
     7681 ggccccaatc acgtcattgt caaagaaatg catgatcact gcaaataagc caaccgccac
     7741 tgcacccaac accagcaatc tgtttgtatt tattttcact ttcggaagtc cttcggctgt
     7801 atctttcgcc ttattgattt tatgaggagc ataaatcccg atagcatttc cgtccggtcc
     7861 tatcaggtat ttatttttct gtgttacata gagtataagg cttaatccca tcatgatcgc
     7921 agcggccata aatccccact tgaaatcggc aggatcgccc gtatcaccca gataaccgca
     7981 caagagcgga gccataaatg cacccacatt gacccccatg taaaaaatag tataggcaga
     8041 atcgagccgt ttgtcgcccc tttcgtacag ctgacctaca atggtggaaa tattgggctt
     8101 gaaaaatccg ttaccgaaaa taagtccggc aagtccaccg tacatgagcc actttgccag
     8161 ttcggtactt tgataattca atgcactgaa aaacatcaat aactgaccca acaccatcag
     8221 cactgcaccc cagaaaatgg atcggcgcat acccaggtaa cggtcggcca catagccgcc
     8281 aacgagcgga gtgaggtaaa ccagcccggt ataattacca taaattgctg aggcaaattc
     8341 cttgtccatc agcagggctt tgattaaata caacacaaaa atggcacgca ttccataata
     8401 gctgaaacgc tccgacatac cggttgcaaa aacgaaataa agccccttag ggtgtgattg
     8461 tttgatattt tctgccatga ttttattttt gatttttaca aaaaaaagaa cgccttggtt
     8521 gttgcgttca tttttcggat tttaaaagtg gtgcaaatgt acataattta ttttaaagat
     8581 agaattatcg ctgtcaacaa gctgcttttc tttatctact tttatcaagt tcgattttta
     8641 cgctctccac actccccccc aggggtggat tgagtttgga aagctgaacc ttaagtgtct
     8701 ttattggggt aaatgtgtcg aatacagcgt ccaatatcct ttgtccggcg tgttctatca
     8761 gattggaagg gatttccatt tcccgtctca ccacatcata cacctgttga tagttcaacg
     8821 tatcttccag attatcggtt ttacccgcaa gtgtggtatc cagctccata attacggata
     8881 ccaaaaacgt atttccttct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 ngcaagtgtg gtatccagct ccataattac ggataccaaa aacgtatttc cttctttttt
     9061 ttcgaaatcc agacaaccgt gataggcgtg gaatttcata ttgtgtaatg tgatgactgc
     9121 catagtagcc tcctaactcc taaaggggga atttttaaaa tatttttata ctaaatcgta
     9181 cgaaactctc tttcatggat ctaaggctta tttcagcaca taatgtgcca gcactttttg
     9241 cacttcgtat acattgaccg agcgggtaaa gtttttgcga atgctgggct gttgctcact
     9301 cgaaatccag attttcagtt cggcttccag atcaaaggta ccggcggatt caatgcagaa
     9361 ccgggaaatg cttttgtagg gaacggataa gtactcctgc ttgctgcccg taattccctg
     9421 tacattaatc attatcatcc gtttattggt gaaaataaat gtatcacgga ttaacctgaa
     9481 tcccatttcg atgttttcat tttcggtcag taaattattg aattcttttt cgagtttcac
     9541 cggatcaatg gtgccggcat ttcctaatag tgctgagaat agtcccatat tgagtaattg
     9601 attttaaaat tgctctgatt atttttttaa agaattaaat ttcgatgtta tcctgtcttc
     9661 cagttgcaaa tatgctttca gtaaaatttt ttattaaacc catggtgacg gttttgttct
     9721 ccacccttgg tgtatgtgca gcctcaggga tgatagcgat ggtaaccggt gcagttatct
     9781 cttttttcag cacattcagt tgctcttcgg tatcataggg gtctttttca ccccgaaaag
     9841 ccaatacagg acagctaatg tcggttaaca gaggaattat actccaatcg gcaaaaaaat
     9901 cgctcaacca ggtctcgtgc caccgtttga aaagttcttg ggttttatcg ccgtgatact
     9961 tccggagtga ctgtaacaat gaattatgtc gggctctgtc gcgattttcc cgaacagctg
    10021 ctttcccttt ttcctcgata aaactgtgtg ctccctccag tattactgca tcaaaccggt
    10081 cgggatagtt ggctgcagca atcaaagcga tagtcgcacc atcgctgtga ccatacaaca
    10141 ctgctttctg aatattcatc ctgtccatga tgcggatgag ttcatcggct tcatcgtgga
    10201 ggtagtattt attgcgttgc gtactgtcaa atggcgagga tttgccgtat ccgcgccgat
    10261 cgtataaaaa ggcattcatt ccggtgattt ccaccatttt tttgggaaaa tcgccccaca
    10321 tttccacgca tccccacgaa tcatgcaaaa atatcaacac gggttcattc cggttttggg
    10381 gatattcaat tctttcataa taaacgccaa aatcactcaa ccacatactg ttaactgttt
    10441 tcataaacct ataattcaca cattccacac ctgcaaaagt acataaaaag ggataaatac
    10501 aggtaaatga ggtatataat atttcgataa agagattttt caagtacttt tgcacgactg
    10561 gttacttttt cagaaccagt tccgtcgcca tgaaaaaaaa attatcaacg gtttttttaa
    10621 cctttattgt ctgcactatt tttgctgcca atccggtgaa gtcgttcacc gacaatgacc
    10681 tgctgcgaaa ggccaacatc agcatcaccg tgaaagacct gaatacgggc aaaatagtgg
    10741 aaagctatcg tccggaatgt gccacggtgc cggcatctac gctcaaactg gtaaccaccg
    10801 caacagcact ggaactcctc gggccggaat ttcggtttag cacgaccctt gaaacagatg
    10861 gactgattac tgccgacagc acactgaacg gaaatttgta cattcatggc agcggtgatc
    10921 ctacgctggg gtcagccatg cagggagata ctgcttttct caccaactgg aaaagcggca
    10981 ttctgaaagc aggaattaaa cggattaacg ggagtattgt agccgatgca acccgcttcg
    11041 atacacaggg cataaatccc aaatggctgt gggaagatat gggaaactac tacggagccg
    11101 gtgcttacgg aatttcatac aaggataatt tattcaccct ggaatttcgc tcagggaaag
    11161 caggcagtac tccggaaatt gtgaaaatga taccagaaat ccccgatttc aaattcgaaa
    11221 acaacctgaa aagttccgct atcgggtttg acagcgctta tttctatgga gcgccttttt
    11281 caaattttag aatgatttac ggtgaaattc cagccaaccg accttcattt aaggtcaaag
    11341 gcgatattcc acgtccggga ttgctgctgg ccagggattt tactgccaaa cttcagtcgg
    11401 cgggaattca ggtgagtggc attcctaccg acgttttgac cggtaataat tcccggagca
    11461 tcatttatac ccatttttca cctccattgc gcgagataat tcgtgaaatt aatgttcaaa
    11521 gcaataacca ttatgccgag catgtttttc gctacctttc gatggtaaac gatacggttg
    11581 caacaagccg tggttcggta tcggtaatcc gaaagctctg ggcatccaaa tcattgccgg
    11641 tggatgaact tttcatgtac gacggctcgg gtttatcacc ggtcaatgcg gtatcgtccg
    11701 gatttatggc cggtttattg gaatatatgg acaaatctcc caacaaagag gttttcaaag
    11761 cttcgttgcc cgtagccggc accacgggca ccctcaaatc atttctgaaa aacacacctt
    11821 tgcagggtaa agtacgggca aaaagcggct ccatacatcg ggtgaaatgt tatgccggtt
    11881 atataaatac aaaaggtaaa aattatgcct tttcgattat ggtgaataat ccgaacggaa
    11941 ctctgagcga aacaactaaa aaaatggaag aatttcttct cacaataacg aaatgacacc
    12001 aacccggtac tacacagcat caaccgcttc gaccaacaca ttgcttcatg aaatgatgcg
    12061 gcaggataag ctgcccgaag gatttctgct gtataccgat tttcagagtg ccggcaaagg
    12121 tcaacccgga aatacctggg aaagtgaaaa cggaaaaaat ctgctgttca gcatgttgct
    12181 atacccgcac tccattaaag tgtacgaaca attcatcctg agtgaaatta ccggtctggc
    12241 catcagaaaa gtgctggaaa aatatacaga tgacatctgt ataaaatggc ccaacgatat
    12301 ttattggaag gataaaaaaa ttgccggtat actgatagaa aattcacttt tcagagaccg
    12361 gattgacaca tgtataattg gtatcgggct gaatgtgaac caggaagtat tcatcagcaa
    12421 tgctcccaat ccggtttcac tccgacagat taccggaaat gatgtaaaca gggaaatatt
    12481 attaaaggaa attcaggctg aattattgaa tatttatcaa aattattcgc ccgaatttat
    12541 tcatcaggaa tacttgaatc atttgtaccg gaggaatggt tttcaccgct atgcagaaac
    12601 cgctgcgaac acagtttttg atgctgaggt tgaagacgtt ttgccggacg gcagattgat
    12661 tttacgtaca caggcaggcg aaatcaaatc gtattatttc aaagaaattc aatttgtgat
    12721 ataaaaagca aagagtcgga aattcatccg actctttgct ttataaataa atgatttatt
    12781 tcaaatttcc cactgttcca gtgaattcac caggtcatcg aagcatttga tttttgattt
    12841 ctcctgtact tcggctannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca
    12961 tcgtgtacca gcaggtcttt ttcggtaatt ccgttttcac caatggtaac agctttcaga
    13021 ttaaatccgt caagaacaag ccctttatct cgattcaccc cataaatcat gggttttccg
    13081 tgttccagaa aaataatgcg atccggtcgg gtctcctttt cggcaatcca tccgtgcgct
    13141 ccgtgattga aaatcacaca gttgaccagg cactccacaa tcgaggtgcc tttgtgacgg
    13201 gcggctgctc ctattgtttc agtagcattt ttcaaatcca catccagcaa acgggcaaag
    13261 aatgtaccac gtgaaccaaa cgtgagttcc gccggacgaa atggacgttc aatagtacca
    13321 aagggagacg attttgttat gctccctttt ttagtggtgg gcgaaaattg tcctttggtc
    13381 agaccgtaaa tctggttgtt gaacaacaat acattcatat ctatatttcg tcgaacgctg
    13441 tgaatgaagt gattgccacc tatagccagt ccatcgccgt ctcccgaaat ctgccagact
    13501 gtcagtttgg gattggcaat tttcacaccg gaagccacag ccgctgcgcg accgtgaata
    13561 gtatgaaatc cgtaactatt gatgtagtaa ggcaggcgcg aagagcatcc gatgcccgat
    13621 attacggcaa tatcctgagg agcaacaccc atatcggcca tcactttctg caaactgttg
    13681 aggatggcgt agtcaccaca accgggacac cagcgaacgt actgatcact tttaaaatct
    13741 ttgggggtat attgttttat ttcttccata gctataaatt atttcagaat taattcaaaa
    13801 tgttctacaa gttctgccat agtaaatggt tgaccctgaa ctttgttgtg ttgaaggaaa
    13861 ttcactcccg gcactttgga gcgcaggaat gaagcaaact gtccgttgtt gagttcgcaa
    13921 acgactactt ttttgtactt tgaaatcacc tcagctgtat tttttggcag cggattgatg
    13981 tagttgaaat gagccagtgc tacttttttc cctttttcat tcatttcaga tacggcactc
    14041 atcaggtgac cgcgggttcc accccatcca attaccaata aatcagcatc ttccgaacca
    14101 gatactgtta gatccggaat aacggcagtc acattttcca ctttttcggc acgggtatcc
    14161 accattttct gatgattttc gccatcggtg gaaatgcttc ccttttcgta gtccttttcc
    14221 agtccgccat ttcggtgttc gaagcccgga gttcccggaa tagcccagta acgcactttc
    14281 gtatcttcgt tgcggtaagt taccttgcaa ccttccatac cgggttgaac gtaattcggt
    14341 tttatttcgg gcatatcggc cagtttcgga agtttccaga gtgaagttcc attgcccaga
    14401 aatgcatctg tcagcaaaat aaccggtgtc atatattcca gtgcaatttt ggaagccatg
    14461 taagcaaaat gaaaacaatc atcgggagtt ccgggcgcca ttaccaccag cggactttcg
    14521 ccatttcggc catagagtgc ctgtaataaa tcggtttgtt ctgttttggt cggcataccg
    14581 gtggaaggac cacttcgcat tacatccaca attacgatcg gcaattcagc catcactgcg
    14641 aggtttattc cttctgattt cagtgccagt ccggggccgg aagtattggt cacagccaga
    14701 cagccggcaa atgcagctcc gatggaagtg gttatgccgg caatttcatc ttcacattga
    14761 accacttttg cacccagatc gcggcgcaaa gccagttcgt gcataatatc agtagccgga
    14821 gttatggggt aactgcccaa aaaaagttct aagcctgctt tttcggcagc ggccacaaat
    14881 ccgtatgcgg ttgccgtatt tccggagatg cttgtatatt ttccgtgagg aacattatcc
    14941 gatttttcta ctgtgtaggt aggaactgtc agatgtaaat tatttccata gttgaaacca
    15001 tcagtaagca cttttacgtt ggcttcgagt accgaaggtt ttttcccgaa tttatttccc
    15061 agaaaatgaa tggcctgatc catcggacgg ttgaataacc agcaaataag ccccagtgca
    15121 aacatatttt tgctgcgaag aatagcttta ttgtcaagcg gatatccttc aagacttttc
    15181 tgggtgagcg tggtgagctg agcaggcacc agactgatgg tgtcggaaat ggataattcc
    15241 tcaaaaggat catcggtcac aaatccggct ttaaccaaat cgctcttttc gaagcaattc
    15301 tcatcataaa tgattacacc gccttttttt acatgtttgg catttacttt gagtgcggcg
    15361 gg
//
LOCUS       DAOPZK010000044        15315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_4166_length_15315_cov_22.440105, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000044 DAOPZK010000000
VERSION     DAOPZK010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4166_length_15315_cov_22.440105"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PKH68_04265"
     CDS             <1..1454
                     /locus_tag="PKH68_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNX12185.1"
                     /translation="LLEELGVDRIEVASARVSEGEFQSVKKIAQWAQTHGHLNRVEVL
                     GFVDGTASLDWISSAGCRVVNLLCKGSLKHCTHQLRKTVEEHLADIKAVIANAGKMGI
                     KVNIYLEDWSNGMRDSEAYVYQMMDELQHQPIMRYMLPDTLGVLNPDETRDFCQRMIS
                     RYPSLHFDFHAHNDYDMAVANVYEAVKAGVKGVHVTVNGLGERAGNAPLTSVIALIHD
                     HLKLHTNVKENNVYHVSKVVESYSGIRIPNNKPVIGDNVFTQVAGIHADGDKKNNLYF
                     NDLMPERFGRTRAYALGKTSGKANILKNLELLGIELDDESMKKVTERIIELGDKKEVV
                     STDELPYIISDILQSDIEKQDIRMLNYSLTLTDGLRPIANVKISIHDEIYEQSAAGDG
                     QYHAFSKAMYKIYKRLNKPTPELIDYVVVIPPGGQTDAFVQTIITWKFDGKTFKTRGL
                     DIDQTVAAIKATMKMLNLIETGNIPTINYMELP"
     gene            1765..2838
                     /gene="leuB"
                     /locus_tag="PKH68_04270"
     CDS             1765..2838
                     /gene="leuB"
                     /locus_tag="PKH68_04270"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNX12186.1"
                     /translation="MKTLKIAVLPGDGIGPEIVKQALDVTLAVCDKFGHKLEYNYAIV
                     GACAIDETGDPYPAATHELCMKSDAVLFGAIGHPKFDNDPNAKVRPEQGLLRMRKTLG
                     LYANIRPVSTFPQLIHKSPLRAELIEGADFMCIRELTGGMYFGRPQGRSEDGNTAYDT
                     CVYTREEVERILHLAYKYAGQRRKKVTVVDKANVIATSRLWRQIAQEIAPQYPDIETE
                     YMFVDNAAMRIIQWPKSFDVLVTENLFGDILTDEASVITGSLGMLPSASIGLHTSVFE
                     PIHGSYPQAAGKNIANPLATILSAALMFEYAFGMMEEGRLIRQAVTASMDAGIVTEDI
                     SEGGKAYSTSDVGAWIVKWIQEK"
     gene            complement(2910..4166)
                     /locus_tag="PKH68_04275"
     CDS             complement(2910..4166)
                     /locus_tag="PKH68_04275"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNX12187.1"
                     /translation="MIVYKFGGASVKSPDGIRNIAKIVSSVQEDLFIVVSAMGKTTNA
                     MEVVLDFFMKAEKESALNKLAEVENYHREITTELFSDKSVAENAVKPLFDELKELITT
                     GIGTDYDRWYDRLVSYGEVISTKIVSEFLLMQGIQNRWLDMRQLLVTDSNFREANVHM
                     LESEKRLKAAVDFDKSKIYIGQGFIGTNMQGSPTTLGREGSDYTAAVVGNLLNAESVS
                     IWKDVPGILNADPRIFPDTVLIPELTYMDAVELAYSGAQIIHPKTIKPLENKSIPLYV
                     RPFGSPSEAGSVIKASTSHPIDIPVLILKKNQMLVTLRPLDFSFILEESLTWIFTVVE
                     KHRLKVGLIQSSAISISICVDNSRYLQRALDELANDYRVSYNDNLELLTIRGTNPEII
                     KRTTEGREILLQQQTRRIGRFLMRQI"
     assembly_gap    4273..4372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4427..5671)
                     /locus_tag="PKH68_04280"
     CDS             complement(4427..5671)
                     /locus_tag="PKH68_04280"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNX12188.1"
                     /translation="MKKLSVFVLLLISISVAGQPVNRTQLLHENIKTLQVKVDENPIC
                     LPVITLNGSEMLQISFDEMSHNIHSYSYSIKHCNADWSLSNLSSTEYIDGFTTGRIDD
                     YALSVNTTFLYTHYRFQLPNNEIKWKLSGNYVVNIFEDNQQDDPIARVCFSVVDPRVQ
                     IEAAVRGNTDTELSGRLQQLDFDVLLNDYRVQDAQSEIKVVVRQNNRTDNQVTDIKPS
                     YLAAGKLSYKNNRSLIFEGGNEYHRFDISSIYNYDEGVSAIDFVRPAYHAYLRQDQII
                     ARNPYLTNPDVNGKFVINLQNASDDNTEADYMYVHFYLPVDAPFFDGKIYVGGEWNYN
                     QMDKTSLMRYDSEHAMYYNTVLLKQGGYNYQYWMIPQGETKAQVSPIDGSHWQTRNEY
                     TIYIYHRPWGGRYDKLIGVKIL"
     gene            complement(5699..7957)
                     /locus_tag="PKH68_04285"
     CDS             complement(5699..7957)
                     /locus_tag="PKH68_04285"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX12189.1"
                     /translation="MKSRIPLIFTALGLLIISSCSNKTSTDYTTLVDPFIGTGGHGHT
                     FPGATYPHGMVQLSPDTRIYDWDACSGYHYSDSLVNGFSHTHLSGTGCADYGDVLVMP
                     VVGEADIRKMPLGSQNTSYASAFSHKKEEAIPGYYSVMLDRYNVKAELTASQRAGFHK
                     YTFPVSDKAGLLIDLDYAIQGQVTKELTLEVISDSEIVGYKKSAYWAPEQYIYFYAKT
                     SKPFTSATFYRNDSIIAGVKEVSGSGKVLLSFATKADEVVMLKVGISAVDTDGARKNL
                     EAEIPEWDFDKIKFATRKAWNDYLAKVDVKTKNSKYKRIFYTALYHTAIAPNLFSDVD
                     GRYRGMDKKIYTSNENMYTVFSTWDTMRALHPLFTIIDPALNQNFIRSLIQKSKEGGI
                     LPMWELAANYTATMIGYNAVPIIVDAYMKGNRDFDVDEAYKAMLKTSSYDTTNIVASY
                     AFKHALMPLSKKYKNELGYIPCDTEHENVAKGLEYAYDDWCILQVAKDRGDTANIARY
                     DKLARNYKNYFDKTTGFMRGKDSKGNWRTPFNPNSSNHRSDDYCEGTAWQWLWFVPHD
                     VDSLVQLLGGKNKFTAKLDQLFKTDSKIEGELVSADITGLIGQYAHGNEPSHHIIHFY
                     NYVGQPYKTQELIDSVLHSQYSDKVDGLAGNEDCGQMSAWYILNAMGFYQVCPGKPVY
                     SIGRPLFDKVTVNLPGNKTFTIKTYNNKRENKYITSIKLNGHKLEKPFFTHAELMAGG
                     TLEMIMSDKPKR"
     gene            8208..9536
                     /locus_tag="PKH68_04290"
     CDS             8208..9536
                     /locus_tag="PKH68_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HNX12190.1"
                     /translation="MNLPEPFVSRTQELLKEELPAFLDALDYPSPVSVRVNDKMEYLP
                     SDDTVKWCDAGYYLAERPVFTLDPLLHAGVYYVQEASSMFLQQALNRFVVPQSVVLDL
                     CAAPGGKSTLISQYLSTHGMLVSNEIIRSRAYILAENLIKWGNDTVVVSNNEPKDFQR
                     LPSFFDAVVVDAPCSGEGMFRKDAGAVGEWSEENVMMCARRQRQILTDVWDTLKKDGI
                     LIYSTCTYNREENEENVSWMETELGAELIRLNTDAFPEITVSDKGYRFFPHKTRGEGF
                     FLSVLRKTSDAPSLRIKKQDRRQQKSEFNETELKNKLIEPENWKIYTDNNLISAFRTK
                     FSDKVEMLKSNLYIMHYGITLYEIKGRDLIPHTSLALSKSIQLENVLQAEVELRDALL
                     FLKKETISLSETEKGFVLIKYRNIPLGWVKNLGNRCNNLYPNEWRIRMKI"
     gene            9574..10398
                     /locus_tag="PKH68_04295"
     CDS             9574..10398
                     /locus_tag="PKH68_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX12191.1"
                     /translation="MNFINDNNSSLTLPLEPVPINREGRRATITFLGTGTSTGVPQLG
                     CKCPVCRSDNPKDKRLRNSALITVDDKRFLIDAGPDLRQQLITNHIFHVTGILLTHEH
                     YDHVGGLDDVRPLGETNIYAEANVLEAIERNMPYCFGAKIYPGVPKMNLHKITTNPFQ
                     IQGVEIIPIRALHARLPVLGFRVGDLAYLTDVKTIEDTELEKLRGLKVLVINALRKEE
                     HISHISLSEAIHIARKVNAENTYFSHISHHLGLHDEVSRELPENIHLAYDNLSINI"
     gene            10472..11533
                     /locus_tag="PKH68_04300"
     CDS             10472..11533
                     /locus_tag="PKH68_04300"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HNX12192.1"
                     /translation="MKSVVTKIFPALGLIFLITSCASTRMYTTLDILRPAQVSFAPEV
                     NNILIVDNSVPQPATVGHYLEKLNSNESQIELQFDSASIFCTASLRENLEEKGFFRNV
                     YQAHNRLNLSGDYAKITPLTPEVVKSLCAVYHADAVLALNRLIYADKITEFTYENEFG
                     FHNYLDVKIRTDWSIQYPTDKPMTYTHFTDSFTWQSSNAIRSKAANELPARYDALVDA
                     SLLTGLNVADRLIPRWEKEDRYFYNPRNKYMRQAMDSVVTRNWQAAILLWKKVADKPG
                     SSSLKFKANNNIAIAYEILGDLDNAIKYCNSAINAYPQLQVFNSYNTIYEIEELLAYK
                     KELEKRAGEIKQIKTQLGN"
     gene            11571..13088
                     /locus_tag="PKH68_04305"
     CDS             11571..13088
                     /locus_tag="PKH68_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNX12193.1"
                     /translation="MLQPNDEQILQKKGISLQQIEEQLRYFVTGFPYLQIRNSASIGN
                     GIIRVNETETQSYLTEWDKYLTSDAVVLKFVPASGAASRMFKDLFGFLEGQSELPVTA
                     FEKKFFDEIEKFAFHDDLNAVCVANSGKTIPELISTHQYKEIVANLLLEKGLNYGALP
                     KGLLKFHSYKDEKRTPMLEHLVEGAVYASTASGQVNIHFTVSAEHRSLFEKHLAENLP
                     RFEKRFGVKYNVHFSEQKPSTDTIAADAENNPFRDKGELVFRPGGHGALIENLNDLHA
                     DIIFIKNIDNVVPDAMKEVTITYKKVIAGVLVHYQQQAFAYLKELENEKISDAKLTVI
                     AQFCEKKLNNHADNLKSLSGNELRQYLMGKLNRPIRVCGMVKNAGEPGGGPFLTVNAD
                     GTVSPQILESSQIDTDNPADKEKMMNSTHFNPVDLVCGVKDYKGRKFDLLKFVDKNTG
                     FISSKSKSGKELKALELPGLWNGAMSDWNTIFVEVPIETFNPVKTVNDLLRKEHQ"
     gene            13581..15140
                     /locus_tag="PKH68_04310"
     CDS             13581..15140
                     /locus_tag="PKH68_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNX12194.1"
                     /translation="MKSRFMFLLCLFYISFHLTANAQEKSLWKMVFEDEFNAQTFNTK
                     YWSYCTRANPDWAKYLTSSPATVNNADGFLKLRLIKNTDTSVDNVPYLSGGIQTKSKF
                     NFKYGKVEVRAKFSNGQGSWPAIWMMPQDASAGWPACGEIDIMEHVNSEDRIHQTIHS
                     DFSNTQGIKNPSPTLTTAFDKNAFNVYGVEWHPDRLDFTLNGKLTFSYPRINTTKTGQ
                     WPFDKPFYIILNMAGGGSWTGAITEAALPFEMQVDWVRVYERDPEGSYIVPGWTSNKS
                     QNNTQNDSYWANTFVKKITTTGALNNLNYETSQRPESYYYEHADTLNVTENSSVSFDL
                     TANSLGDYTTSTVLQDLRYTCNYVYADFDGDKRFETSLERIGNVPPTNGVGGNFNVMN
                     ISRTLNIPQNSSGRTGRVRIIYHNAWSDRLSPETSVFEGLVYDFPIRIKQKDTKVQQI
                     EHRPAVYRHGEYVISENIDGNYKVELFNASGQCLQVDHYRDTNFRIQAPKGFVIVRIT
                     NSLGYSFSYKL"
BASE COUNT         4345 a         3268 c         3385 g         4217 t
ORIGIN      
        1 tgctgctcga agaactgggt gtggaccgca ttgaagtggc ttcggcacgg gtttcggaag
       61 gtgaatttca atcggtgaaa aaaatagcgc aatgggcgca aactcacgga catctcaacc
      121 gggtggaagt gcttggattt gtggatggaa cggcgtcgct cgactggatt tccagtgccg
      181 gttgccgggt agttaatctg ctttgcaagg gttccctgaa acattgcact catcagcttc
      241 gcaaaacagt ggaagaacat ctggcggaca tcaaagcggt gattgccaat gccggaaaaa
      301 tgggtattaa agtcaatatt tacctggaag actggtcgaa cggtatgcgc gactccgagg
      361 cctatgtcta tcagatgatg gatgaactgc aacatcaacc gattatgcgc tatatgctgc
      421 ccgatacgct gggcgtactc aatccggacg aaacccgcga cttttgccag cggatgataa
      481 gccgttaccc ctcgcttcac ttcgactttc atgcgcacaa cgattacgac atggccgtag
      541 ccaacgtgta tgaagctgtg aaagccggag taaaaggcgt tcacgtaaca gtaaacggac
      601 tgggcgaaag ggccggaaat gccccgctga ccagtgtcat tgcactgata cacgatcacc
      661 tgaaactgca caccaacgta aaagaaaaca acgtgtacca tgtgagcaaa gtggtggaaa
      721 gttattcggg tattcgtatt cccaacaata aaccggtgat aggcgataat gtattcacgc
      781 aggtggcagg aattcatgcc gatggcgaca agaaaaacaa cctttatttt aacgacctga
      841 tgcccgaacg tttcggtcga acccgggcgt atgcgctggg taaaacatcg ggtaaagcca
      901 atatcctgaa aaacctggaa ctgctgggca ttgagctgga tgatgaaagc atgaaaaaag
      961 tgactgaacg gatcatcgaa ctgggcgaca aaaaagaagt ggtgagtacc gatgaattgc
     1021 cctatattat ttcggatata ttgcagagcg atattgaaaa acaggacatc cgaatgttga
     1081 actattcact tacgctcacc gacgggctga ggccaattgc caatgtaaaa atttccattc
     1141 acgacgaaat ctacgaacaa tcggcagccg gcgacggaca atatcacgcc ttctccaaag
     1201 ccatgtacaa aatttacaaa cggctcaata aacccacacc cgaacttatt gactacgtgg
     1261 tggtaattcc gcctggcgga caaaccgatg cgtttgtaca aaccattatc acctggaaat
     1321 ttgacggaaa aacattcaaa acacgcggtc tcgacatcga ccaaactgtg gcagccatca
     1381 aagcaaccat gaaaatgctc aacctgatag aaaccggaaa tatcccgacg ataaattata
     1441 tggagttgcc ataatggcgc ggccccctaa cccccctaaa gggggaatat tggcaggtaa
     1501 aatcagagtt gatgattacc ggaaaatttg acaattactt tggaacaaca gataacccag
     1561 gagtttttaa ctttacagaa atattagaat aaacataatc aacgctttct tagttccccc
     1621 cttcagggga gtagggggca gatatttaca aacttaattt atgctttcta aattcccccc
     1681 tttagggggt tagggggcaa attcaaacaa ttagaatcaa cgctttctta gttccctctt
     1741 tgggggttag ggggctaatc atttatgaaa acattaaaaa tagccgttct tccgggcgat
     1801 ggtatcggtc cggaaatagt aaaacaagcg ctggatgtaa ctctggccgt atgcgacaaa
     1861 tttggtcata aactggaata caactatgcc atagtaggtg cctgtgccat cgacgaaacc
     1921 ggcgatcctt atccggctgc cacgcacgaa ctctgcatga agtccgatgc agtactattc
     1981 ggtgccatcg gccatccgaa atttgacaac gacccgaatg ccaaagtgcg tccggagcaa
     2041 ggcttgctgc gtatgcgcaa aacactggga ttgtatgcca atatccgacc ggttagtaca
     2101 ttcccgcagt taattcataa atcgccgctt cgtgccgaac tcattgaagg cgccgacttt
     2161 atgtgtatcc gtgaactcac cggtggcatg tatttcggac ggcctcaggg acgcagcgaa
     2221 gatggaaata ccgcttacga tacctgcgtg tatacccgcg aagaagtgga acgcattctg
     2281 cacctggcct acaaatatgc cggtcagcgc agaaagaaag taactgtggt ggataaagcc
     2341 aatgtaatag ccacgtccag attgtggcgt cagattgcgc aggaaattgc cccgcagtat
     2401 cccgacattg agaccgaata catgtttgtg gacaatgctg ccatgcgtat cattcagtgg
     2461 cctaaaagtt tcgatgtgct ggtaaccgaa aatcttttcg gcgacattct taccgacgaa
     2521 gcctctgtga ttaccggttc gctgggaatg cttccttccg cctctatcgg gttgcacaca
     2581 tcggtttttg aacccattca cggttcttat ccgcaggcag caggaaaaaa tattgccaat
     2641 cctttggcaa caattttatc agccgcattg atgtttgagt atgctttcgg gatgatggaa
     2701 gaaggccggc tcatccgcca agcggtaact gccagcatgg atgccggtat tgttaccgaa
     2761 gatatttccg aaggtggaaa agcgtattcc acgagcgatg tgggcgcgtg gatagtgaag
     2821 tggatacagg aaaagtaatg aattttacca aaaaactcag gaggcggcta tagtaattag
     2881 tcgcctcctg agttataaaa ttcaaacact tatatttgtc tcatcaagaa tcttccaatt
     2941 ctgcgggttt gctgctgcag caaaatttcg cgtccttcgg tggttcgttt tataatttcg
     3001 ggattggtac cgcgaatggt cagcaattcc aggttgtcgt tgtagctcac gcggtagtcg
     3061 ttggccagtt catccagtgc gcgctgcagg tagcggctgt tgtccacaca aatagaaatg
     3121 ctgatggccg aactttgtat caatcccact ttcagccggt gtttttccac cacagtaaaa
     3181 atccaggtga gactttcttc caatataaat gagaagtcaa gcggacgcaa agttaccagc
     3241 atctgatttt ttttgagaat aagtaccggt atgtcaatgg ggtgggaggt ggatgctttg
     3301 atgaccgagc cggcctccga agggcttcca aacggacgaa catagagcgg aatggacttg
     3361 ttttcgagag gcttaattgt tttggggtga ataatctgag caccgctgta agccaattct
     3421 acggcatcca tataggtgag ttccggaatt agcactgtgt cggggaaaat tcgcggatca
     3481 gcgttgagta tgccgggaac atctttccag atggatacgc tttcagcatt gagcaaattg
     3541 cctactacgg cagccgtgta gtccgaacct tcgcgaccga gggtggtagg ggaaccctgc
     3601 atatttgtcc cgataaatcc ctggccgatg tatattttcg atttgtcgaa atcaacagct
     3661 gctttcagcc ttttttcaga ttcaagcata tgcacatttg cttcgcggaa gttgctgtcg
     3721 gtgaccagta actgacgcat atcgagccat cggttttgaa ttccctgcat tagcaaaaat
     3781 tccgacacta ttttagtgga aataacttca ccgtatgaaa caagtcgatc gtaccaacgg
     3841 tcgtaatcgg tgccaatgcc cgtagttatg agctctttga gttcgtcgaa cagcggcttt
     3901 actgcatttt cagccactga tttatcactg aaaagttccg ttgtaatctc gcgatggtaa
     3961 ttttcaactt ctgccagttt gtttaaagca ctttcttttt ctgctttcat gaaaaaatcg
     4021 agtaccactt ccatggcatt ggtggtttta cccatggccg aaaccacaat aaacaggtct
     4081 tcctgtaccg atgaaactat ttttgcaatg tttctaattc cgtccggcga ttttacggac
     4141 gctccgccaa atttatatac gatcattgtt atgtggttat taagcccccc taccccccga
     4201 aagggggttc aagaaaagac agtttttggt tattaagtta ttaagctatt ggttattgaa
     4261 atagggttat tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattaggtt
     4381 aaagaataaa acatcaaaca tcaaacatta aacattaaac atgaaactat agaatcttca
     4441 cacctattaa tttgtcatat cgtcctcccc agggtctgtg gtaaatgtag atggtgtatt
     4501 cgttgcgtgt ttgccagtga ctgccatcaa tcgggcttac ctgtgctttt gtttctccct
     4561 gaggtatcat ccagtactga taattgtaac ctccctgctt gagaagaact gtgttgtagt
     4621 acattgcatg ttcgctgtcg tatctcatga gtgatgtttt atccatttga ttgtaattcc
     4681 attcgcctcc tacgtatatt ttcccgtcga aaaaaggtgc atccaccggc agataaaaat
     4741 gcacatacat atagtcagct tcggtgttat catcgctggc attctgcaaa tttatgacga
     4801 attttccgtt tacatccgga ttagtcaggt agggatttcg ggcaatgatt tgatcctgtc
     4861 gcagataagc atgataggca ggcctgacaa aatcgatggc actcacccct tcgtcgtagt
     4921 tgtagataga cgagatatcg aagcggtgat attcatttcc cccttcaaaa atcaggctgc
     4981 ggttgttttt ataactcagt ttgcctgcag ccaggtagct gggtttgata tctgtcacct
     5041 gattgtcggt gcggttattc tgccgaacga caaccttgat ttccgactgt gcatcctgaa
     5101 cccggtaatc attcagcagt acatcaaaat cgagctgttg cagtcgcccg ctcaactcgg
     5161 tatcggtatt tccccgaacg gctgcctcta tttgcacccg gggatcaacc accgaaaaac
     5221 aaacccgggc aatcgggtca tcctgctgat tatcctcgaa gatattcacc acataattac
     5281 ccgaaagttt ccattttatt tcattatttg gaagctgaaa ccggtaatgg gtgtataaaa
     5341 aagtagtatt taccgagagc gcataatcat ctatgcgtcc ggtggtaaat ccgtcgatgt
     5401 actcggtcga tgacaaattg gaaagcgacc agtccgcatt gcagtgcttt attgagtacg
     5461 agtaggagtg tatgttgtgc gacatttcat caaaacttat ctgcagcatt tcactaccat
     5521 taagggtaat gacaggcaag catattggat tttcatctac cttcacctgc agggttttga
     5581 tgttttcgtg caatagttga gtgcggttaa caggttggcc tgcaaccgaa atgcttatca
     5641 gtaaaagtac aaatacactc agttttttca ttttcaaatt acgttggatt tagcgtgttt
     5701 accgttttgg tttgtcggac atgatcattt ccagtgtacc tccggccatc agctcggcat
     5761 gtgtaaagaa tggcttttcc agtttatgtc cgttcagttt tatggatgtg atgtatttgt
     5821 tttcacgctt attattgtaa gtttttattg taaatgtttt attcccgggc aaattgacag
     5881 ttaccttgtc gaacagcggg cgcccgatac tgtaaaccgg ttttccggga caaacctgat
     5941 aaaatcccat ggcattgagg atgtaccagg ccgacatttg tccgcaatct tcgttaccgg
     6001 ccagtccgtc cactttgtcg gaatactgcg aatgcaacac gctgtcgatt agttcctggg
     6061 ttttgtaggg ctgacccacg taattgtaaa aatgaataat gtggtggctc ggttcgttgc
     6121 cgtgggcata ctgtccgatc agacccgtaa tatctgcaga tacaagttca ccttcaattt
     6181 tggaatctgt tttgaacaac tggtcaagtt ttgctgtgaa tttatttttc cctcccaata
     6241 actgtaccaa actatccaca tcgtgcggca caaaccacag ccactgccag gcagtacctt
     6301 cgcagtaatc gtcggagcga tgatttgaac tgttcggatt gaaaggtgta cgccagttgc
     6361 ccttgctgtc ctttccgcgc ataaatccgg tggttttatc aaaataattt ttataattcc
     6421 gtgccagttt gtcatatctt gcaatattgg cggtatctcc ccggtctttg gctacctgca
     6481 atatgcacca gtcatcgtag gcatattcca gtcctttggc tacattttcg tgttctgtat
     6541 cacacggaat ataaccaagt tcgtttttat acttttttga taatggcatg agtgcatgtt
     6601 tgaatgcata ggatgctaca atattggtgg tgtcataact tgacgttttc agcatggctt
     6661 tgtaagcctc atccacatca aaatcgcggt tacctttcat gtaagcatcc acaataattg
     6721 gcacagcatt gtacccgatc atggttgctg tgtaattggc agccagttcc cacatgggca
     6781 gaattcctcc ttctttcgat ttttggatga gcgatctgat aaaattctgg ttcaacgccg
     6841 gatcaataat tgtgaacagc ggatgcaatg cccgcatcgt atcccacgtg gagaacacgg
     6901 tgtacatatt ttcatttgag gtgtagattt ttttatccat accgcggtat cttccgtcca
     6961 catctgagaa aagattggga gctattgcgg tgtgatagag ggccgtataa aaaatccgtt
     7021 tgtatttgga gttttttgtt tttacatcca cttttgccag gtagtcattc caggctttgc
     7081 gtgtggcaaa tttgattttg tcaaagtccc attcgggaat ttctgcttcc agatttttgc
     7141 gggctccatc tgtatctact gctgaaatac ccactttcag catcacgact tcatccgctt
     7201 tggtcgcaaa tgagaggagc acttttccgc ttcccgacac ctctttgaca ccggcaatga
     7261 ttgaatcgtt gcggtagaat gtagccgagg taaatggttt tgaagttttt gcatagaaat
     7321 aaatatactg ttccggtgcc cagtaagccg atttcttgta accaactatt tctgaatcgc
     7381 tgattacttc gagagtaagc tctttggtaa cctgtccctg tatggcatag tccaggtcga
     7441 taagcaaacc tgctttatcg gagacaggga aggtgtattt atgaaatccg gcccgttgcg
     7501 aagcggtaag ttcggctttc acattgtacc ggtcgagcat tacggaataa taacccggaa
     7561 ttgcttcttc ctttttgtgt gagaaggcag atgcataaga tgtgttttga ctgccgaggg
     7621 gcatttttcg gatatcagct tcgcccacta ccggcatcac cagtacgtcg ccgtaatcgg
     7681 cacatccggt gccgctcagg tgcgtgtgcg aaaaaccatt gacaagcgag tctgaataat
     7741 gataaccgga gcaggcatcc cagtcgtaaa tgcgggtatc gggactgagc tgcaccatac
     7801 cgtgcggata ggttgcaccc ggaaatgtat ggccgtgacc tcctgttcca ataaaaggat
     7861 cgaccagggt ggtgtaatcg gtcgaagttt tattgctgca agaactgatt attaacaatc
     7921 ccagagctgt aaaaatgagc ggaatgcgtg atttcatagt gacttattta atggaaattc
     7981 cggttgttta attcgaatgc aatattaatc aatttttgtt gaatatgtaa attcacacgg
     8041 gtgatcctgg aaaaataaaa tgggacaaat taacaggtaa aaaaatgggt attgttcacc
     8101 tgactgccgg acaaagagta ttattacatt ccgaataatt tttacctgtt gcaaatttac
     8161 acataaatca cgaaatttat gtaagtttgc acattaaaaa gtcccgcatg aatttacccg
     8221 aaccatttgt cagtcgtact caggaattat taaaagaaga attacccgca tttctggatg
     8281 cactcgatta cccgtcgcct gtgagtgtac gtgtgaatga taaaatggaa tatttacctt
     8341 ccgacgatac ggttaaatgg tgtgatgccg gatattatct ggctgaacga ccggttttca
     8401 ccctcgatcc actgctgcat gccggtgttt attatgtcca ggaagcttct tccatgtttt
     8461 tgcagcaagc tctcaatcgg tttgttgtgc ctcagagtgt ggtgctcgac ctgtgtgcag
     8521 cacccggcgg aaagtccaca ctgatatcac aatacctgag tacgcacgga atgctcgtca
     8581 gcaacgagat tattcgcagc agggcgtaca ttctggccga aaatctgata aaatggggca
     8641 atgatacggt ggtggtttcc aacaacgagc caaaggattt tcagcgtttg ccttcctttt
     8701 tcgatgcggt ggtggtggat gctccctgct cgggtgaagg aatgtttcgc aaggatgcag
     8761 gagcggtcgg cgaatggagc gaagagaatg taatgatgtg tgcccgaagg cagcgccaaa
     8821 tcctgaccga cgtgtgggat acgctgaaaa aggatggtat tttaatttac agcacttgta
     8881 cttacaacag ggaagaaaat gaagaaaatg taagctggat ggagacagaa ctgggtgctg
     8941 aattgattcg gttgaataca gatgcttttc cggaaattac cgtttcggat aaaggttacc
     9001 ggttttttcc tcacaaaacc cgcggtgaag gtttttttct ttccgttttg cgaaaaacgt
     9061 cggatgcacc ctcactccga ataaagaaac aggatagaag gcaacaaaag tcggaattca
     9121 atgaaactga acttaaaaac aaactcattg aacctgaaaa ctggaagatt tataccgaca
     9181 ataatctgat ttcagcattc agaacaaaat tctccgacaa agtagaaatg ctgaaaagta
     9241 atttatacat aatgcattat ggtattaccc tctatgaaat aaaaggtcgt gatttaattc
     9301 ctcataccag tctggcatta tcaaaaagta ttcagttgga aaatgtactt caggccgaag
     9361 tcgaactgag ggatgctttg ttgtttctta aaaaagaaac catcagtttg tctgaaacag
     9421 aaaagggatt tgtacttatt aaatatcgga acataccgtt agggtgggtt aaaaacctcg
     9481 ggaatcgctg caataatctt tacccgaatg aatggcggat caggatgaaa atataaaaag
     9541 caactaaaac attgttttct gaaaattaaa aaaatgaatt ttattaatga taacaacagc
     9601 tctttaactc tccctttgga gcctgtcccg attaatcggg agggtcggag ggctactatc
     9661 actttcctgg gcaccggaac ttccaccggc gttccacagc ttggctgtaa atgccccgtc
     9721 tgccggtcgg ataacccaaa ggacaagcgt ctccgcaatt ccgcacttat tacagttgac
     9781 gataagcgat ttctcattga tgccggacct gacttgcgtc agcagttaat caccaaccat
     9841 attttccacg tcacaggcat tctgctcact cacgagcatt acgaccacgt gggaggactg
     9901 gatgacgtgc gcccgctcgg tgaaacaaat atatatgccg aagccaatgt gctggaagct
     9961 atcgaacgta atatgccata ctgtttcgga gcaaaaattt atcccggtgt accaaaaatg
    10021 aatttacaca aaataaccac aaatccattt caaattcagg gagtggaaat cattcccatc
    10081 cgcgctctgc atgcacgact tccggtgttg ggcttccgcg ttggagatct ggcgtatctt
    10141 accgatgtga aaactattga agatacggaa ttagaaaaat tgcggggtct aaaggtgttg
    10201 gttatcaatg cacttcgaaa agaagaacat atttcgcaca tctcgctgtc ggaagccatt
    10261 catatagcac ggaaagtgaa tgccgaaaat acctatttct cgcatataag tcatcacctg
    10321 ggtttgcatg atgaagtcag ccgtgaattg cccgaaaata ttcacctggc ttatgataat
    10381 ttatcaataa atatttaact ttgcagtaaa caacttggtt accaaagagg ttttttagtt
    10441 gaaccttttt ccccaaaaaa acacccagag catgaagtct gttgttacta aaatattccc
    10501 ggcattagga ttgatttttc tgataacttc ctgtgccagc acccgcatgt acactacgct
    10561 ggacatactt cgcccggcgc aggtttcctt tgcaccggaa gtaaataata tactgatagt
    10621 ggacaattca gtgccacaac cggcaactgt tggacattat ctggaaaaat taaattcgaa
    10681 cgaatcacag atagaacttc agtttgatag tgcttccata ttctgtacgg caagtcttcg
    10741 cgaaaacctg gaggagaaag gatttttcag aaatgtatat caggctcata accgcctgaa
    10801 cctctccggc gattatgcca aaatcacacc gctgacacct gaagtggtta aaagtttgtg
    10861 tgccgtatat catgccgatg ccgttctggc actcaaccga ttgatttatg ccgataaaat
    10921 tacggaattt acctacgaaa atgaatttgg ctttcacaac tatctggatg tgaaaatacg
    10981 aacagactgg tctatccagt atcctaccga caaaccaatg acttacacac actttactga
    11041 tagttttacc tggcagagca gtaatgccat tcgaagtaaa gctgccaatg aacttcctgc
    11101 ccgttacgat gccctggtgg atgcatcgct gcttacgggg ctcaatgtag ccgaccgcct
    11161 cataccccgc tgggaaaaag aagaccgcta cttctataat ccacgcaata agtacatgcg
    11221 tcaggcaatg gattcggtag tgacccgcaa ctggcaggca gccatcctgc tttggaaaaa
    11281 agtggctgat aaacccggaa gttcatcact aaaatttaaa gcaaataaca atattgctat
    11341 tgcttacgaa attctgggtg acctcgacaa tgccatcaaa tattgcaatt cagccatcaa
    11401 tgcctatcct caattgcagg ttttcaattc atacaacacc atctacgaaa ttgaagaatt
    11461 gttggcttac aaaaaagagc tcgaaaaaag agccggggaa attaaacaga taaagacaca
    11521 actcggcaat tagatatgaa acctgttaat cctaatcaat actaaaaact atgcttcagc
    11581 caaatgatga acaaattctg cagaaaaagg gaatttctct gcagcaaatc gaagaacaac
    11641 ttcgttactt cgtaacgggt tttccttacc tgcagatcag aaattcagca tcgattggta
    11701 atggaattat aagggtaaat gagactgaaa cccaatctta tctcaccgaa tgggacaaat
    11761 acctgacttc cgatgcggta gtgctgaaat ttgtaccggc ttcgggagca gccagccgca
    11821 tgtttaaaga cctgtttgga tttctggaag gtcaaagcga actccctgtc actgcttttg
    11881 aaaagaaatt cttcgatgag atagaaaaat ttgcctttca cgatgatttg aatgccgttt
    11941 gtgtagcaaa ttcggggaaa acgatacctg aactcatcag cactcatcag tacaaagaaa
    12001 ttgtggccaa tctgttgctg gaaaaaggac tgaattacgg cgcactcccc aaaggtctgc
    12061 taaaatttca ttcgtataag gatgaaaaac ggacaccgat gctcgaacac ctggtggaag
    12121 gagctgtgta tgcctcaaca gcttcggggc aggtaaatat tcattttacg gtgtcggctg
    12181 agcaccgtag cctgttcgaa aaacacctgg ccgaaaatct tccccgattt gaaaaacggt
    12241 ttggtgtcaa gtacaatgtt catttttcgg agcaaaagcc atcgaccgat accatcgcag
    12301 ccgatgccga aaataatccg ttccgcgata agggagaact ggttttccgt cccggtggtc
    12361 atggtgcact catcgaaaac ctgaacgacc tccacgccga tattattttt atcaaaaaca
    12421 ttgataatgt agttcccgat gcaatgaaag aggtgactat tacctacaaa aaagtgatag
    12481 caggcgtgtt ggtacactat cagcagcagg catttgctta cctgaaggaa cttgaaaatg
    12541 aaaagatatc ggatgccaaa ctcaccgtca tagctcagtt ctgcgaaaag aaactgaata
    12601 atcacgccga caacctgaag tcactttcgg ggaatgaact gcggcaatac cttatgggca
    12661 aactgaaccg gcccattcga gtatgcggta tggtgaaaaa tgccggagaa cccggcggag
    12721 gtccattcct taccgtcaat gccgatggaa ctgtgtctcc gcaaatcctc gaaagttcgc
    12781 aaattgacac agataatcct gccgataagg aaaaaatgat gaattccacc cacttcaatc
    12841 cggtggattt ggtgtgcggt gtgaaggatt acaaagggcg aaaatttgac ctgctaaagt
    12901 ttgtggataa gaacaccggt tttatttcct ccaaatcaaa aagcgggaaa gaacttaaag
    12961 ccctcgaatt gccgggtctg tggaacggag ccatgagcga ctggaatacc atttttgtag
    13021 aagtgcccat cgaaaccttc aatccggtaa aaactgtcaa cgatttgctg cgcaaagagc
    13081 accagtagga tacgtatata acaatgaaaa aaggatgttt tttgacgaac atcctttttt
    13141 gttgaataat gtgaattcta tacagaggaa tgtacactct aacacccgaa tgtgacgtcc
    13201 caacgtggag tgtttttcaa caggcaaatt tttcccgcaa accagtaaag ccccaaagaa
    13261 ggtgtttttt ttattcattt attccggttt tttttctgta gggttggggg caatcgatta
    13321 tgttatacgg agacctctaa tttgtattca catgaatata acccgatatt tgggcactca
    13381 aatataattt aatatatttg caaccaaatt tggtttttgg tgacaatcac taaccatttt
    13441 atgaggcaat ttctccggac tctgcttcat ttttttttgc cgtcaaccgg atggatactc
    13501 ttatccccgt ttgccgataa tactatgata atactatgat aatattaaga ttctgaattt
    13561 attaattaaa tctaaggtat atgaaatcac gttttatgtt tttattgtgt ttattttaca
    13621 tttcttttca tttaacagca aatgctcagg aaaaaagtct gtggaaaatg gtttttgagg
    13681 atgaatttaa tgcccaaact tttaacacta aatactggag ttattgtaca cgtgcaaatc
    13741 ctgactgggc aaaatatctc acatcgtcgc ctgcaacagt gaacaatgct gatggcttcc
    13801 tgaaactcag acttatcaaa aataccgata ccagtgtgga caatgtgcct tatttgagtg
    13861 gtggaattca aactaagtct aaatttaact ttaaatacgg caaggtggaa gtacgtgcta
    13921 aatttagtaa tggtcagggt tcctggccgg ccatctggat gatgcctcag gatgccagtg
    13981 ccggctggcc tgcctgtgga gagattgata ttatggaaca cgtgaattca gaagacagaa
    14041 ttcaccaaac aatccattca gatttttcaa atacacaagg aatcaaaaac ccgtccccaa
    14101 cgctgacaac ggctttcgac aaaaatgcct ttaatgtata tggtgttgaa tggcaccctg
    14161 acagactcga ttttacacta aatggcaagc ttacatttag ctatccgcgt atcaatacca
    14221 ccaaaaccgg tcagtggcca ttcgacaaac cattctatat tattctgaat atggctggtg
    14281 gcggcagctg gacaggcgct atcaccgagg ctgcgcttcc attcgaaatg caggtggatt
    14341 gggtacgtgt gtacgaacgc gatcccgaag gatcatatat tgtacccgga tggacatcga
    14401 ataaatctca aaataatact cagaacgaca gttattgggc taataccttt gttaagaaaa
    14461 ttacaacaac tggtgctctg aataacctga attatgagac ttcacagcgt cctgaatctt
    14521 attattacga acatgccgat acgttaaacg tcaccgaaaa cagtagtgtt tcttttgacc
    14581 tgacagcaaa ttcactgggc gactatacta caagtaccgt acttcaggat ttgaggtaca
    14641 cctgcaacta tgtgtatgcc gattttgatg gtgataaacg tttcgaaact tcgctcgaac
    14701 gtattggaaa tgtgcctcct actaatggag tgggaggaaa ttttaatgtc atgaacattt
    14761 cgcggacact gaatatccca cagaatagtt caggtcgtac aggacgggtg agaataattt
    14821 accataatgc ctggtccgac aggttatcac ccgaaacaag cgtttttgaa ggtttggtct
    14881 acgattttcc catacggatt aaacaaaaag atacaaaagt gcagcaaatt gaacaccgtc
    14941 cggcagttta ccggcatgga gaatatgtta tatctgaaaa tattgacgga aattacaaag
    15001 tagaactttt caatgcaagc ggacagtgct tacaggttga tcattatcgc gatacaaatt
    15061 ttagaataca agcacctaaa ggttttgtaa ttgtcagaat taccaactcc ttagggtatt
    15121 cattcagtta taaattgtag ggatcttttt tttgttgggt aaaatgtctt gttgggtaaa
    15181 atgaaacgaa atacgcaggt attgaagtgg agttttacca ctctcagttt attaaagtat
    15241 tttttttgat ctggatatcc ggatggtgag cctttttggg gactcgaacc ccagacctat
    15301 tcattacgaa tgaat
//
LOCUS       DAOPZK010000045        15305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_4173_length_15305_cov_21.567410, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000045 DAOPZK010000000
VERSION     DAOPZK010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15305
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_15305_cov_21.567410"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2111)
                     /locus_tag="PKH68_04315"
     CDS             complement(<1..2111)
                     /locus_tag="PKH68_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX12195.1"
                     /translation="MKRNFFLLLLLLCIQFTQADLLTDITDGKFKPKSLSAIQSMNDG
                     EHYTVMQDDDMIIKYNYKSGKAVDTILDLNKIKKSPLVNIKGYTFSPNEAKILVYSNP
                     QYRYRRTFTANYYIFDVKRNELKPLSENGPQEAPLFSPDSRYVAFARANNLFLYKIDF
                     GTESPITKDGEFGKIINGTPDWVYEEEFVETRHFVFSPDSKLLAFIKFNETDVPTYSM
                     QHFLDSPIRQDELPLYPSVQTFKYPNPGQNISKVSVCVYDDFYKSVKTINLDTSEKDY
                     YIPRIKWTADQEKLAVFVLNRNQNRLDMLIANPKSLLSKLILRDEDNSYVDYDNIDAV
                     FFTPDNKYFTYLSEKDGYRHAYLYNVNGTLSRQLTKGSWDVTNVYGYDAATQTLYYQS
                     AESSPLQRDIYSLDAKGKKLRLTDGKGTHDALFSKSFAYFVDNASTANNPTVSIMRDK
                     KGIKIRTMEDNATLASEFKALNLPVKEFFTFTTSEGIKLNGWMLKPVGFNPAEKYPTL
                     MVQYSGPNSQEVLDRWKIEWEYFLSTKGYVVVSVDGRGTGARGAEFRKCTYQQLGVLE
                     ARDQIEAARYLATQSFVDKDRIGIWGWSYGGYMTILAMSSGEKIFKAGIAVAPVTDWR
                     LYNAAYTERFMRTPQENNRGYDAGSVLNKAGKLNGNLLLIHGTADDNVHLPNTMIYLD
                     KLMKAGKHADLQLYTDKNHSIL"
     gene            2400..3251
                     /locus_tag="PKH68_04320"
     CDS             2400..3251
                     /locus_tag="PKH68_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX12196.1"
                     /translation="MRSIKHFFNNSESNNPNETIGLCLSGGGALGFAHIGVLQALHDY
                     RIFPQIISGTSMGSIIGSLYAAGYTPAQMIKFIDEGKLYRVTKLMTFQPAFWKAGLTS
                     QAAIMSLINDLIPNNSFEKLEKPMHICVSNLTTGKYEIIEHSNKLDMWIGASCSIPGI
                     FNGMKINDMTYVDGGLLNNFPAQPLREKCSVIIGSDVIPHSSVMKNQKSRDTLLSSLR
                     VGIHQNSLPGREMCDYLIEPKAIEKFHEFSFENFQSIYHYGYKATVQFIADNPEILRL
                     QQPIQPD"
     gene            complement(3359..3700)
                     /locus_tag="PKH68_04325"
     CDS             complement(3359..3700)
                     /locus_tag="PKH68_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12197.1"
                     /translation="MEETKTVYDAVPDYRTQSQQGQKETALPKLSFCILMDLAGLATY
                     ALPALGEWGDMLWAPISGLIFMKTFGGMTGVIGGIANMAEELIPFTDFIPTFTIGYFY
                     TKYQLNKASKK"
     gene            complement(3834..5216)
                     /locus_tag="PKH68_04330"
     CDS             complement(3834..5216)
                     /locus_tag="PKH68_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX12198.1"
                     /translation="MKTKMIISGLALILLVSCNSSVRENKDAYQPKPYVELTHPEWSK
                     NATIYEVNIRQYTPEGTFRAFESHLPRLKAMGVDILWLMPVHPIGEKNRKGTLGSYYS
                     VKDYRAVNPEFGTMDDLKSLVKKAHDMGFHVILDWVANHSAWDNNLVTEHPEWYTRSL
                     DGNFQPTPWYDWDDIIDFDYNQPGLRKYMTEALKYWVKEADIDGFRCDVAGFIPVDFW
                     DNARRELEEIKPVFMLAEWEDRDMVKNAFDMVYSWSLWGKMKEATTGGKGAGALIEYM
                     AHDVSTFPENGYRMIFTDNHDKNSWEGTQFTNFGKGLETCMVLSTVVNGMPLVYSGQE
                     AGLDRPLKFFEKDTIVWKNHPFAGIYTKLFDLKHRNKALWNGKWGGTMVRVYNDQQDK
                     VVSFYREKEGNKVLAAFNFSDKPVAVTLKTKHETGSYTDLFSGNQVEIKGEDLLQLKP
                     WGYAVYSIEK"
     gene            5370..6176
                     /gene="mazG"
                     /locus_tag="PKH68_04335"
     CDS             5370..6176
                     /gene="mazG"
                     /locus_tag="PKH68_04335"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNX12199.1"
                     /translation="MNTEYKAHSNEEKVKEFERLLNIMTRLRAECPWDKEQTFDSLRT
                     NTIEETYELADAILSGDMPEIRKELGDLLLHIVFYAQMGSETNDFDIYDVCNGLCEKL
                     IYRHPHIFGDVVAHDSEMVVQNWEKLKLKEKGGNRTVLAGVPDALPALIKAHRMQDKA
                     RSVGFDWEHRDQVWEKVKEEIDEFQAEINRMDKDKMEDEFGDVMFSLVNAARLYDINP
                     ENALERTNRKFAARFNYLESQTIAKGKDLKNMTLAEMDEIWEEAKKKKDY"
     gene            6301..8808
                     /locus_tag="PKH68_04340"
     CDS             6301..8808
                     /locus_tag="PKH68_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNX12200.1"
                     /translation="MHTSENTIENITLKTLSNLDATNGRLYPDTFEQKISFNTIRQLL
                     KDRCLCSIGEEMVDKMQFSSNYDEVLQWISQTDEILRLISTNNEDLPVGDFFDVREAL
                     SRVQVVGLYLDETEIFNLRRSLEAVRKLVLFIARREPEQFPELHQLTAGIHSFEAITK
                     RIDSILDKFGKVKDSASAELNRIRKDIFRVQSGISKTLLGILRQAQADGYVEKDVSPT
                     MREGRLVIPVSPSFKRKVSGIVHDESATGKTVFIEPAQVVEANNRVRELESEERREVI
                     RILVEFTDFLRPYFEDIIASQYFLGVIDFLRAKSLFAIEIKAIKPRVDDVCQLEWNQA
                     VHPLLYLTLKKQHKPVVPLDITLNEKQRILLISGPNAGGKSVCLKTVVLLQYMLQCGM
                     LVPFHENSRAGIFEKLFIDIGDEQSIENDLSTYSSHLLNMKYFIKNSNDKTLILIDEF
                     GSGTEPQIGGAIAEASLERFNLNKSFGVITTHYTNLKHFAEDAEGIVNGAMLYDRQHL
                     QPLFQLQIGNPGSSFAVEIARKIGLPEDLIADAAKKVGLEHLDYDKHLQDIVRDKRYW
                     ERKRMEIRQKEKRLEETLEKYETELGLTEKKRKEITTRAKAEAENLLKETNAKIENTI
                     RTIIEAQADKEKTRTVRKELEEFKIKLTEKKIPTSKPKAKFEVKKSVIEPKIENRITF
                     EPGSLVRLKGQTAVGTILELHEKQAVVAFGQLKSTVKLSQLEAASNHQSKKEKRKFES
                     LGTPASDEVRQRKLTFKSEIDVRGMRGDEALQAVIYFIDDAVMVGVASVRILHGTGTG
                     ALRQLIRQYLNTVSGVIRFRDEHVQFGGAGITVVEFE"
     gene            8902..>10061
                     /locus_tag="PKH68_04345"
     CDS             8902..>10061
                     /locus_tag="PKH68_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNX12201.1"
                     /translation="MKNCLYKTLVFTLLAFNWLNASAVPASPYPVSINQPDGTTLTVY
                     LRGDEFHHYQTTEDGVVILKNKNGVYNYARVDATGKISDTSIKAKNVKDRNDSEKLLV
                     SEFGNSTMLNKVSSQMRTKKVSASKKVVRSSVYPNTGSPKSLVILVNFSDVPFVTPSP
                     QSAFSKLLNEPGYSANGGTGSARDYFKDNSMGTFSPDFVVVGPYTLPNTLDYYGKNDT
                     DGYDQNAIQMIVDACKLANENGLDFTQFDTDNDNLVDNVFVYYSGYNEAENAPDNTVW
                     PHRWGIYPNSVYGNGNYTGSTASVTFDGKRVEDYACTSELQGKANANMCGIGTFTHEF
                     GHVLGLADMYATDGGTHHTLDYWDIMDYGPYLNSGRTPPSYSAFERFSLGYLT"
     assembly_gap    10062..10161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10162..10865
                     /locus_tag="PKH68_04350"
     CDS             <10162..10865
                     /locus_tag="PKH68_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12202.1"
                     /translation="AWGKNITLLPIDSVVSPTNIQIRYNPTEPSYLSTHYDNLQFTTP
                     FAEKLSIPLSGKSSRPVYVVPPVAASAADVTYRSFVASWNSVNDATGYYLTVYNVSDE
                     GITDTIVSRKWITATSDTLYNLVSNRNYFYKVQASDKNTLYGYENITNFSNIVKVRSA
                     SYPLKKELRVVYSNGSVRVFVPSRTSIVNVFNMIGQKIMSFTANSDIPEISNLRKGQV
                     YIIQADDYRAKVLVH"
     gene            complement(10940..11206)
                     /locus_tag="PKH68_04355"
     CDS             complement(10940..11206)
                     /locus_tag="PKH68_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12203.1"
                     /translation="MKQITDKNITIEDIPAFKQEAKQKIEVQKVRLITTAQDLVPFSS
                     STTSTITPLALITAPFRKGKAMTVVQGVILGYNLIRKLRQFLKK"
     gene            complement(11187..11576)
                     /locus_tag="PKH68_04360"
     CDS             complement(11187..11576)
                     /locus_tag="PKH68_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNX12204.1"
                     /translation="MATKSGYINSFTQLFTEVKDYLKLQGDYVKIDIVEKITKILSML
                     LFILVVLVLAMGLLFYLLFSLAYILGPEIGFVSSFAIIAGIYLILLVVMVIFRKSLII
                     NPLLRIIVGMFYEEEKIETEGNETDNG"
     gene            complement(11582..11830)
                     /locus_tag="PKH68_04365"
     CDS             complement(11582..11830)
                     /locus_tag="PKH68_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNX12205.1"
                     /translation="MKTTGGGILAALLGGAVVGAALGLLLAPEKGEETRAKIVEGFED
                     TKSKVVEALKKRGINLSKSDLEDLVDELKSEADAKTTV"
     gene            complement(11922..12803)
                     /gene="era"
                     /locus_tag="PKH68_04370"
     CDS             complement(11922..12803)
                     /gene="era"
                     /locus_tag="PKH68_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNX12206.1"
                     /translation="MHKSGFVNIVGNPNVGKSTLMNALVGERLSIITSKAQTTRHRIL
                     GIVNGDDFQIVYSDTPGVLKPNYRLQESMLNFSRSALSDADVLLYVTDVYDNSDKNTD
                     FVEKVKQNPASVILVINKIDLINEEKLVELVEKWKTVLPQAEIFPISATEKFNIEPLL
                     KRIQELLPEGEPFFDKDQLTDKPAKFFVTEIIREKILLNYDKEIPYSVEVEVEQFQEE
                     EKIIRISAVIYAERDSQKGILIGHGGKSLKKVGTESRKDIEAFFEKKVFLELFVKVEK
                     DWRNKDSILKNFGYKPE"
     gene            12957..14114
                     /locus_tag="PKH68_04375"
     CDS             12957..14114
                     /locus_tag="PKH68_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX12207.1"
                     /translation="MNKVYQTNQILKFSFIVGAMLIVLASTIFTNRLAGKLSVEEQKK
                     IEIWAEATRQLILADETTDMNFILKIIEGNTTIPVIITDENDSMIDYRNIKAPVKNSE
                     DFFRTKIIQLKNKKPKLEIALKETNTKQYIYYDDSMLLKQLYIFPYVQFGIIFSFFII
                     VILAFASTKRAEQNKVWVGLSKETAHQLGTPISSLLAWIELLKLRYQNDTLINDMEKD
                     VNRLRVIADRFSKIGSKPDLKKSDLRETLTNAVNYMNNRSSNKIEISLHFASELPVTV
                     PLNVPLFEWVIENLCKNAIDALNGAGKINLNVKNSDNEVIIDVSDTGKGLERNLYQAI
                     FSPGFTTKERGWGLGLSLAKRIIEEYHKGKIFVKHSEVNVGTTFRIILPTN"
     gene            14220..14792
                     /locus_tag="PKH68_04380"
     CDS             14220..14792
                     /locus_tag="PKH68_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNX12208.1"
                     /translation="MSKFEQYNIPLKGLKTESQVFEFDLDDNYFKKIDSPEVQHGKIK
                     ATVTVRAKQGLFDLNFVLNGVIQIPCDRCLDEMNQPIQYKEKIQVKFGDKFSEEDDIV
                     IVPETDGGINVAWFLYEFIVLNIPIKHVHAPGECNKNMVGRLKKHITRQRIDDDDPDD
                     LEFNEEDEIPTDESISDPRWDGLQNIFENN"
     gene            14805..14990
                     /gene="rpmF"
                     /locus_tag="PKH68_04385"
     CDS             14805..14990
                     /gene="rpmF"
                     /locus_tag="PKH68_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNX12209.1"
                     /translation="MAHPKRKQSKQRTAKRRTHDKAKLPTLATCSNCGATHIYHTVCP
                     ECGYYRGKLAIEKIAAE"
BASE COUNT         4654 a         3125 c         3029 g         4397 t
ORIGIN      
        1 aggatgctgt ggtttttatc agtatagagt tgcagatcgg catgtttgcc cgccttcatc
       61 agtttgtcga ggtaaatcat ggtattgggt aaatgcacat tatcatcggc tgtgccgtgg
      121 ataaggagga ggttgccatt cagtttaccg gctttattga gcacagaacc ggcatcgtaa
      181 ccccggttat tttcctgggg agtgcgcata aaccgttcgg tgtaagcagc gttatacagt
      241 cgccagtcgg tcacaggtgc cactgctata cctgccttga aaatcttttc gccggaactc
      301 atggctaaaa tagtcatata gccaccataa ctccagcccc agataccaat gcggtcttta
      361 tccacaaaac tttgagtagc caggtatcgg gctgcttcaa tctgatcgcg ggcttcgagt
      421 actcccagtt gctgataggt gcatttccgg aattcggctc cgcgagctcc ggttcctctc
      481 ccatccacac ttactactac ataacctttt gtgctcagaa agtattccca ttctattttc
      541 caacggtcaa gcacttcctg tgaatttggt ccgctgtact gcaccatcag tgtgggatat
      601 ttttcagccg gattaaatcc tacaggtttc agcatccagc cattcagttt tattccttcc
      661 gaagttgtaa atgtaaaaaa ttcttttaca ggcagattaa gtgctttgaa ttcggaagcc
      721 agtgtcgcgt tatcttccat tgtccggatt tttattcctt ttttgtcacg catgatgctt
      781 actgtcgggt tattcgcagt ggatgcatta tcaacaaaat aagcaaatga tttgctaaat
      841 aaagcatcat gagtaccttt accatcggtc aggcgtagtt tctttccttt tgcgtccagg
      901 gaataaatat cgcgttgcag gggtgaactt tcggccgatt gatagtagag agtttgggtt
      961 gctgcatcgt atccgtacac gttggttaca tcccagctgc ctttcgttaa ctgcctggac
     1021 aaggtaccgt ttacgttata aagataggca tgacgataac catccttttc gctgaggtat
     1081 gtaaaatact tattgtcagg ggtaaagaat actgcatcaa tattatcata atccacatag
     1141 gagttatctt cgtcacgcag aatgagtttt gagagcagtg attttggatt ggcaatgagc
     1201 atatcgaggc ggttctgatt tctgttcaga acaaaaacgg cgagtttttc ctggtcggca
     1261 gtccatttta tgcggggtat ataataatct ttttcgctgg tatccaggtt gatagttttt
     1321 actgatttgt aaaaatcgtc gtaaacacat acactcactt tagaaatatt ctgcccgggg
     1381 ttgggatatt tgaatgtctg tacggaggga taaagcggca attcatcctg tctgatggga
     1441 ctgtccagaa aatgctgcat gctgtaagtg ggtacatcgg tttcattgaa tttgataaaa
     1501 gccagcaatt tgctgtcggg gctgaataca aaatggcggg tttctacaaa ttcttcctca
     1561 taaacccagt cgggtgttcc attgatgatt ttaccaaatt caccatcttt agttatcggg
     1621 ctttcggtgc caaaatcaat tttatacaga aaaagattat ttgcacgggc gaaggccaca
     1681 taccggctgt cgggtgagaa aagcggtgct tcctgcggtc cgttttcgga taatggcttc
     1741 agctcattgc gctttacatc aaaaatatag taatttgccg taaaagtgcg acgatagcga
     1801 tattggggat tggaataaac cagaattttg gcttcattgg ggctaaaagt gtatcctttt
     1861 atattgacca agggcgattt ttttattttg tttaaatcca gaatggtatc aacggcttta
     1921 cccgatttgt aattgtattt gatgatcata tcgtcatcct gcatcacggt ataatgttca
     1981 ccgtcgttca tagactggat agctgacagt gattttggct tgaattttcc gtcggtgatg
     2041 tcggtgagca aatcggcttg tgtaaactga atgcatagta aaagaagcag aagaaaaaag
     2101 tttcgtttca ttttttttta tttttataac caggactatt ggaattatgc cttcctcaaa
     2161 tcataattgg ggtttttgaa aaaaaaggca gaactaaaga atctgaacag tgagtaaata
     2221 ttttttatgt gatttcattt gactaaaaat gaacatgaat tctctcagaa cttataattt
     2281 cacacaaaaa taccttaaaa atatgggtat tacccctaaa atgagtattt ttttggttcc
     2341 cttaacaaaa aaagaaaaat ctttgaatta tctttgcaca ttaaatcaca cacaaaacaa
     2401 tgagatctat taaacatttt tttaataatt cagagtcgaa taaccccaac gagacgatag
     2461 gtctctgtct gagtggaggt ggagctttgg gttttgctca cataggagtg ctgcaggcat
     2521 tgcatgacta ccggattttt cctcagatta tctccggaac aagtatggga agtattatcg
     2581 gatcgctata tgctgccggt tatacgcctg ctcagatgat taagtttatt gatgaaggta
     2641 aactttatcg ggttaccaaa ctgatgacct tccaacctgc cttctggaaa gcgggactta
     2701 ccagccaggc tgctatcatg tcgctcatca acgatctgat acccaacaac agttttgaaa
     2761 agctggaaaa acccatgcat atttgcgttt caaatcttac aacgggtaaa tacgagatta
     2821 tagaacatag caataaactg gatatgtgga ttggtgcttc gtgcagcatt ccgggcatat
     2881 tcaacgggat gaaaattaac gatatgacgt atgttgatgg cggattactc aacaactttc
     2941 cggctcagcc gctgcgcgaa aaatgttcgg taattatcgg ctctgatgtt attccgcatt
     3001 catcggtaat gaaaaatcag aaatcgcgcg acacattgtt gagctcactt cgtgtgggaa
     3061 ttcaccagaa ttcactaccc ggacgtgaaa tgtgcgacta cctgatagag cccaaagcta
     3121 ttgaaaaatt tcatgagttc agcttcgaaa attttcagtc tatctatcat tacggttata
     3181 aagccactgt tcaattcata gccgataatc ccgaaatttt aagacttcag cagcccattc
     3241 aaccagatta ggcttaaatc agggaagttt ttttcaaaaa acacaaaaag cacaaaggtt
     3301 cgcacgatta attcatgcga accttttgtt tattgaaaaa aaaataagac aatccgtttt
     3361 acttttttga tgctttattc agttgatatt tggtgtaaaa atatccaatc gtaaacgtag
     3421 gaataaaatc ggtgaaagga attaattcct cggccatatt ggctatacct ccgattactc
     3481 cggtcattcc gccaaatgtt ttcataaaaa taagacccga tatgggcgcc cagagcatat
     3541 cgccccactc gcccagcgca ggcaaagcat acgtggcaag tccggcgagg tccatcagaa
     3601 tacaaaatga gagtttaggt aaggcagttt ctttttgccc ctgctgcgac tgagtccggt
     3661 aatccggcac agcatcgtac acagttttag tttcttccat tccggttaaa gtaaaggtgt
     3721 taattgttta atgtgaaaaa aaagattttg aattgaactt tacaaccaat tcataaatcg
     3781 tgccgaattt tgttaatatc acatccgggc gtcatttccg ctataaaata tttttacttt
     3841 tctatcgaat aaaccgcata tccccagggt ttgagctgca agaggtcttc accttttatt
     3901 tcaacctgat ttccggagaa taaatcggta tatgatccgg tctcgtgttt ggtttttaat
     3961 gttacagcca ccggtttgtc gctgaaatta aatgctgcca atactttgtt gccttccttt
     4021 tcgcggtaaa atgaaacgac tttgtcctgc tgatcattat aaacccgtac cattgttcca
     4081 ccccattttc cgttccagag tgccttattt cggtgtttca aatcaaatag tttggtgtat
     4141 attccggcga agggatgatt tttccaaaca atggtgtctt tttcgaagaa tttcaacggg
     4201 cggtcaagac cagcttcctg accgctgtaa accaacggca taccattcac cactgtgctc
     4261 agcaccatac aggtttccag accttttccg aaattcgtaa actgagttcc ttcccaggaa
     4321 tttttgtcgt gattatcggt gaatatcatg cggtaaccat tttcgggaaa agtgctcaca
     4381 tcgtgtgcca tatattcaat aagagctccg gctcccttgc cgcctgtggt agcttctttc
     4441 attttgcccc agaggctcca tgaataaacc atatcaaacg catttttgac catatctcgg
     4501 tcttcccatt cggcaagcat aaacacaggt ttaatttctt cgagttcacg gcgggcatta
     4561 tcccaaaaat ccaccggaat aaaaccggct acatcgcagc gaaaaccatc aatatcggct
     4621 tcctttaccc aatatttgag tgcttcggtc atgtattttc gcagtcccgg ctgattgtag
     4681 tcaaagtcaa tgatatcgtc ccagtcatac cagggtgtgg gctgaaaatt gccgtcgagt
     4741 gagcgagtgt accattcagg atgttcggtg accagattat tgtcccaggc cgaatgatta
     4801 gccacccagt ccagaatgac gtgaaagccc atatcgtgcg cttttttcac cagtgattta
     4861 agatcgtcca tcgttccgaa ttcgggattg acagcccgat aatctttcac ggagtaataa
     4921 cttcccagtg ttcctttccg gttcttttca ccaataggat gtacaggcat cagccacaga
     4981 atatccacac ccatcgcttt taatcgcggc agatgacttt caaaagcacg gaatgttcct
     5041 tcgggcgtgt actgacgtat gtttacttca taaatagtgg catttttact ccattcggga
     5101 tgtgtgagct ccacataagg ctttggctga taagcatctt tattttctct gacggaagaa
     5161 ttacaggaca ccaacagaat gagtgccaac ccactgataa tcattttcgt tttcataagt
     5221 attcattttg atattattga atgcaaatat aacttttaaa ttccggaatt attccttctt
     5281 tcaattttca tattttaagg ttgggcaata tctcattcaa tctgatttta gaaatatatt
     5341 cttaactttg cagtttataa atgattgaaa tgaacacaga atacaaagca catagcaacg
     5401 aagagaaagt gaaggagttt gaacggctgc tgaatatcat gacccgactg agagcggaat
     5461 gtccgtggga taaggaacaa acttttgaca gcctgcgaac caatacgatt gaggaaactt
     5521 acgaactggc tgatgcaatt ttgtcgggcg acatgcccga aatacgcaaa gagctgggtg
     5581 acttgctgtt gcacatcgta ttttatgctc aaatgggttc cgaaaccaat gattttgata
     5641 tctatgatgt ttgtaatgga ctgtgtgaaa aattgatcta ccgtcatccg catattttcg
     5701 gcgatgttgt agctcacgac agtgaaatgg tggtgcaaaa ctgggaaaaa ctcaagctaa
     5761 aggaaaaagg aggcaaccgt acggtgcttg ccggcgttcc cgatgcatta ccggcactca
     5821 tcaaggcaca ccggatgcag gataaagctc gcagtgtagg ctttgactgg gaacaccgcg
     5881 atcaggtttg ggaaaaagtt aaggaagaaa tcgatgaatt tcaggctgaa attaaccgca
     5941 tggacaagga caaaatggaa gacgaattcg gagatgtgat gttcagcctg gtcaatgcgg
     6001 cacggcttta cgacatcaat cccgaaaatg cactggaacg taccaatcgt aagtttgcgg
     6061 ctcgtttcaa ctacctggaa tcacaaacca tagccaaggg aaaagatctg aaaaacatga
     6121 ctttggctga gatggacgaa atttgggaag aagccaaaaa gaaaaaggac tattagatta
     6181 tcagagccag aactttaaaa cagaaataag gataaaagtc cctacttgtc acattatttc
     6241 gggaaactta ccataaaagg agactttaag ataagcttta ccaaaactta aaccgttgca
     6301 atgcacacca gcgaaaatac aattgaaaat ataaccctaa aaacactatc aaatcttgat
     6361 gcgacgaatg ggcggcttta tcccgatact tttgaacaaa aaatatcgtt caataccatc
     6421 cgacagttgc taaaagaccg gtgtctgtgt tccattggcg aagaaatggt ggataaaatg
     6481 cagttttcat ccaactacga tgaagtattg caatggatta gtcagacgga cgaaattctg
     6541 cggttaatat ccacaaacaa tgaagacttg ccggttggtg atttttttga tgtccgcgag
     6601 gcactctcac gcgtacaggt agtgggttta tacctcgacg aaactgaaat tttcaacctt
     6661 agacgttcgc tggaagcggt tcgcaaactg gtacttttta tagcacgccg cgaaccggaa
     6721 caattccccg aactgcatca actcacagcc ggcatccatt catttgaagc catcacaaaa
     6781 cgcattgaca gcattctgga taaattcgga aaagtgaagg acagcgcttc tgctgaactc
     6841 aaccgaatta gaaaggatat tttcagagta caaagcggca tctccaaaac cttactcggt
     6901 attctacgtc aggctcaggc tgacggatac gtggaaaaag acgtttcacc caccatgcgc
     6961 gaaggccggt tggtaattcc ggtatcgccg tcattcaaac gaaaagtcag tggcattgtt
     7021 cacgatgaaa gtgcaacagg taaaaccgtg tttatcgaac cggcacaggt agtggaagcc
     7081 aacaaccgcg ttcgtgaact ggaaagcgag gaaagacgcg aagtaatccg aattttggtg
     7141 gaattcactg actttctgag accatatttt gaggatatca ttgcttcgca atattttttg
     7201 ggtgtcattg attttctccg tgccaagtca ctttttgcca tcgaaatcaa agctataaaa
     7261 ccacgtgtgg atgatgtatg tcagctggaa tggaaccagg ccgttcatcc cttgctgtat
     7321 ctgacattga aaaagcaaca taaaccagtt gttccgcttg acattacact gaatgaaaag
     7381 caacggatac ttcttatttc gggtcccaat gccggaggaa aatctgtttg cctgaaaacg
     7441 gtcgtgctgc tgcaatacat gcttcagtgc ggaatgttgg taccctttca cgaaaacagc
     7501 cgtgccggaa tttttgagaa attgtttatc gatataggcg atgaacagtc catcgaaaat
     7561 gatctttcga cgtacagttc acacctgctg aacatgaagt atttcataaa aaacagcaac
     7621 gacaaaacac ttattttgat agatgagttt ggctcgggta ccgaaccgca aatcggcgga
     7681 gccattgcag aagcaagtct cgaacgattt aacctaaata aatcattcgg agtgattacg
     7741 acccattaca ccaatctgaa acatttcgct gaggatgccg aagggatagt gaatggagcc
     7801 atgctctacg accgccagca tctacaaccc ctttttcaac ttcaaatcgg caatcccggg
     7861 agttcgtttg cagtggaaat agcccggaaa atcggtttac ccgaagactt gatcgctgat
     7921 gcagccaaaa aagtgggact ggaacatctg gattacgata aacacctgca ggatattgtg
     7981 cgcgataaac gttactggga acggaaaagg atggaaataa ggcaaaaaga aaaacggttg
     8041 gaagaaacac ttgaaaaata tgaaacagaa ctcgggctga ccgagaaaaa acgaaaggaa
     8101 ataaccaccc gggccaaagc agaggcagaa aatttactga aggaaaccaa cgctaaaatt
     8161 gaaaatacca ttcgtacaat catagaagca caggccgaca aggaaaaaac ccgaactgtt
     8221 cgtaaagaac tggaagagtt taagataaaa ttaaccgaaa agaaaatccc cacaagcaag
     8281 ccaaaagcaa aattcgaggt caaaaaatca gttattgaac ctaaaattga gaatagaatt
     8341 acattcgaac caggttcatt agtacggttg aaaggacaga cagctgtcgg caccatcctg
     8401 gaattgcatg aaaaacaagc tgttgtggct ttcgggcaat taaaatcaac tgttaaactc
     8461 tcccaacttg aagccgccag caatcatcag tcaaaaaaag aaaaacgaaa atttgaaagt
     8521 ctgggcacac ctgcaagcga tgaagtccgt cagcgaaaac ttaccttcaa atcggaaatt
     8581 gatgtacgcg gcatgcgtgg cgacgaagcc ctgcaggctg tcatctactt tatcgacgat
     8641 gccgtgatgg taggtgtggc aagtgtacgc attctgcatg gcacaggcac cggagccctc
     8701 cggcagctta tcagacagta tttgaacact gtaagcggtg ttatccgctt tcgcgacgag
     8761 catgtgcagt ttggtggggc cggtattacg gtggttgaat ttgaataaac gttggcgtga
     8821 tttttgtgtt ttaaattttg ctttatctta attgaaaaaa ttgtatcttt gtatcctttg
     8881 acacttaatt aaattcattg aatgaaaaat tgtttatata aaacattagt ttttacactt
     8941 ctggcgttta actggttgaa tgcaagtgct gtacccgctt caccctatcc tgtatcaatc
     9001 aatcaacccg atggaacaac actgacggtg tacctgcggg gtgatgaatt tcatcattat
     9061 cagactaccg aagatggagt tgtaatcctg aaaaataaaa atggcgttta taattatgca
     9121 cgggtagatg caaccggtaa aatatcggac acaagtatca aggccaaaaa tgtcaaagac
     9181 agaaatgaca gtgaaaaatt gttggtttct gaatttggaa attctacgat gctgaataaa
     9241 gtcagcagtc agatgcgtac aaaaaaagtc tcagcctcca aaaaagttgt tcgttcatct
     9301 gtgtacccca ataccggttc tccaaaatca ctggtaatat tagtaaattt ttccgatgtg
     9361 ccgttcgtca ctccctcacc tcaatctgca ttttcaaaac tgctcaatga gccgggttac
     9421 agtgccaacg gaggtaccgg ttcagcccgc gattatttca aagacaattc catggggact
     9481 ttttcgcccg attttgtggt tgtcggacct tatacactgc ccaatacgtt ggattattac
     9541 ggaaaaaatg acacagacgg atacgatcag aatgccattc agatgatagt ggatgcctgt
     9601 aaacttgcca atgaaaatgg attggacttt actcaattcg atactgacaa cgacaatttg
     9661 gtggacaatg tttttgttta ttactcaggt tacaacgaag cagaaaatgc acctgacaac
     9721 actgtttggc ctcaccgatg gggtatttat ccaaattcag tctatggcaa tggaaattat
     9781 acaggcagca cggcttcagt cacttttgat ggcaagcggg tggaagatta cgcctgtact
     9841 tcggaactac agggcaaagc caatgcaaat atgtgtggta ttggcacatt tacccatgaa
     9901 ttcggccatg tactgggatt agctgacatg tatgccactg atggaggtac tcatcataca
     9961 ttagattatt gggatataat ggattatggt ccttacctga acagcggccg aacacctcca
    10021 agttattctg cctttgaacg cttttcactg gggtatttaa cnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn ncagcctggg gaaaaaacat caccctgttg ccgatcgatt
    10201 cagtcgtatc gcccactaat atccagataa gatacaaccc tacagagcct tcgtatctga
    10261 gcacacatta tgacaatctg cagtttacca ctccttttgc tgaaaagttg tctattccgc
    10321 tgtccggcaa atcttcacgc cctgtctacg tagttcctcc cgtagctgca agtgccgccg
    10381 atgtgaccta cagaagtttc gtagcaagct ggaacagtgt taacgatgcg acaggatatt
    10441 acttaaccgt ttacaacgtc tccgacgaag ggattactga cactattgta tcacgtaagt
    10501 ggattacagc tacatcagac acgttgtaca atcttgtttc aaaccgtaat tatttctata
    10561 aagtacaggc aagtgataaa aacacgctgt acggatacga gaatataacc aacttttcaa
    10621 atatcgtgaa agtacgaagt gcatcctatc ctctcaaaaa agaactgagg gtggtttaca
    10681 gcaatggtag tgtcagggta tttgttccat caagaacttc gatagtaaat gtattcaata
    10741 tgattggtca aaaaataatg agttttacgg caaattcgga tatacctgaa atttcaaacc
    10801 tccgaaaagg tcaggtttat attattcagg ccgatgatta cagagccaaa gtactggtgc
    10861 attaaactca caacaggata aaaaaaggaa tggctccgat tatcagatca gagccattct
    10921 tattttaaac ttatacgatt tactttttta aaaattgtcg taattttcgg atcagattat
    10981 aacccaaaat tacaccctga actactgtca tcgcttttcc tttgcgaaat ggagctgtaa
    11041 tgagcgcaag cggagtaatt gttgaggttg tagaagacga aaatggtaca agatcctgag
    11101 ccgtggtaat taaccgaact ttctggactt caattttctg ctttgcttcc tgcttaaaag
    11161 ctggtatatc ttcaatggta atatttttat ccgttatctg tttcatttcc ctcagtttct
    11221 attttttctt cttcataaaa catacctaca ataatcctta gcagtgggtt tataatcaag
    11281 gatttacgaa aaataaccat gacaacaagt aaaatcaggt aaatacctgc aataatggca
    11341 aaacttgaaa caaaaccaat ttcaggaccc aatatataag ccagtgaaaa taacagataa
    11401 aaaagcaaac ccatagccaa aaccaataca accagtataa acagcaacat ggaaaggatc
    11461 ttcgtaatct tttccacgat gtcaattttc acataatcac cctgaagctt cagataatct
    11521 ttaacttcag taaataattg tgtgaaactg tttatgtaac ctgatttagt tgccatgttt
    11581 tttatacggt tgttttagca tcggcttccg atttaagttc atcgaccaga tcttccaaat
    11641 ccgatttact taaattaatt cctctttttt tgagtgcttc caccactttt gatttagtat
    11701 cttcgaaacc ttccacaatt tttgctctgg tttcttctcc tttttcggga gccaacaata
    11761 atccgagtgc agcaccgact acggctccac ccaataaagc agccaaaatg ccaccacctg
    11821 ttgttttcat aattataatt tttttaagat taaaaatgga tattaattca aaatcaaatt
    11881 tataacactt ttagcagctt aaaggtttag attttacaga attattcagg tttgtatccg
    11941 aaatttttca gaatactatc cttatttcgc cagtcttttt ccacttttac aaacaactcg
    12001 agaaatactt tcttttcaaa aaaagcttca atatcttttc gtgattctgt tcctactttc
    12061 ttgagcgatt ttccaccatg accaatcaga atgccttttt gcgaatctct ttcagcatat
    12121 atcaccgcac ttatccggat aattttttcc tcttcctgaa attgctccac ttcaacttcc
    12181 accgaatagg gaatttcctt gtcgtagttg agcagtattt tctcacggat aatttcagtg
    12241 acaaaaaact tagccggctt atcggtcagt tgatctttgt caaaaaaagg ctcaccttcg
    12301 gggagcaact cctgtatccg ctttaacaac ggctcaatat taaacttttc agtagccgaa
    12361 atgggaaaaa tttcagcttg tggcagtact gttttccatt tttctaccaa ctccaccaat
    12421 ttttcctcgt taattaaatc tattttatta ataaccagaa tgacagaagc cggattttgt
    12481 tttactttct ctacaaaatc ggtgttttta tctgaattat cgtatacatc tgtaacgtaa
    12541 agcagcacgt cggcatcgct caatgccgaa cgcgaaaaat tgagcatcga ctcctgtaac
    12601 cggtaattag gcttcaatac acccggagta tcagaataca caatttgaaa atcgtctccg
    12661 tttacaattc caaggatacg atgacgggtt gtctgagctt ttgaggtgat tattgataaa
    12721 cgttcgccta ccagggcgtt cattaaggtt gactttccta cattgggatt gccaacgata
    12781 ttcacaaatc cggacttatg catattattc ttttttacaa agataacatt tttcaaaata
    12841 acgaacgcaa taatcaccca ttctggtaaa ataaaggagg ttctatcaaa ttatttaccg
    12901 gttttgatac aattttgcta tttttgtcgt tgttaagcaa tataaataca tccggcatga
    12961 acaaggttta ccaaactaat cagatactta aattttcatt cattgtggga gctatgctga
    13021 ttgtgctggc atcaaccatt ttcaccaacc ggctggccgg taaactttcg gtagaggaac
    13081 aaaagaaaat tgaaatctgg gccgaagcta cccgccaact tatactcgcc gacgaaacca
    13141 cagacatgaa tttcattctc aaaatcattg aagggaatac cactattcca gttattatta
    13201 cggatgagaa tgatagtatg attgattata gaaatattaa agctccagtc aaaaatagcg
    13261 aagatttttt taggactaaa ataattcaat taaaaaacaa aaagccaaag ctggaaattg
    13321 cattgaaaga gacaaacaca aaacagtata tttattacga tgattcgatg cttctcaaac
    13381 agctgtacat tttcccttat gttcagtttg gcattatttt ctcattcttt atcattgtaa
    13441 tacttgcctt tgcaagcacc aaacgtgccg aacagaataa agtctgggtc ggtctgtcca
    13501 aagaaactgc ccatcaactg ggaactccca tttcttcgct cctggcatgg atcgagttac
    13561 tcaagctacg ttaccagaat gatacactga taaatgacat ggagaaagac gttaatcgat
    13621 tgagagttat tgcagaccgt ttttcaaaaa tcggttcaaa accggatttg aaaaaatcag
    13681 atttgagaga aacgcttacc aatgctgtca actacatgaa taatcgaagc tccaataaaa
    13741 tagaaatatc actccatttt gcatccgaat tgcccgttac tgttccgttg aatgttcccc
    13801 tgtttgaatg ggtcattgaa aatctttgca aaaatgccat tgacgccctc aacggtgccg
    13861 ggaaaattaa cctgaatgtg aaaaactctg acaacgaagt gatcatcgat gtatccgata
    13921 ccggcaaagg tttagagcgc aacctgtatc aggcaatatt ttcaccgggt ttcacaacca
    13981 aagaaagagg ttggggactt ggtttatcac ttgcaaaaag aataattgaa gaatatcaca
    14041 aggggaaaat atttgtcaaa cactctgagg ttaatgtagg tacaaccttt agaataattt
    14101 tacctaccaa ttaatatatt ccttcgttaa tttgatatat ctttgcagaa attttcagta
    14161 aaacgtttat ttatagcacg aaattttgta cctttgcaag gttttttata gtacacatta
    14221 tgtcgaaatt tgaacaatac aacattccac tcaagggatt gaaaaccgaa tcacaggttt
    14281 ttgaattcga tttggatgat aattacttca aaaaaatcga tagtcctgaa gttcagcatg
    14341 gcaaaattaa agccacagtg actgttcgcg ctaagcaggg tttgtttgat ttaaattttg
    14401 tattgaacgg agtaattcag ataccatgcg acagatgcct ggatgaaatg aatcaaccca
    14461 tacagtacaa agaaaaaatt caagtcaaat ttggtgataa attttcagag gaagatgata
    14521 ttgtcatcgt accagaaacc gatggtggca tcaatgtagc ctggttccta tacgagttta
    14581 ttgtactaaa tatccctatc aagcacgttc atgcgcccgg cgaatgcaat aaaaacatgg
    14641 taggccggct gaaaaaacac attaccagac aaagaattga tgacgatgat cctgatgatc
    14701 ttgaatttaa cgaagaagat gaaattccaa ctgacgaatc tatttctgac ccgagatggg
    14761 acggattaca aaacatattt gaaaacaatt aaataataac taaaatggca catcctaaac
    14821 gaaaacaatc caaacaaaga accgcaaaaa gaagaaccca cgataaagct aaactgccta
    14881 cactggcaac atgttctaac tgcggtgcaa ctcatattta ccataccgtt tgccccgaat
    14941 gtggttacta cagaggtaaa ttagcaattg aaaaaattgc tgcggaataa ataaaatttt
    15001 atgcaatttt ctgattgaag acaggaaatt actttctcac caaaatatta aagagccttc
    15061 tgtactccat gttcagaagg ctctttgggt tgtatacacc tgatatcacc ttctatccat
    15121 ttcaaaaatt ccaaccgatc gtattttatc aataagtgat gcgcaaccaa atgctgtaat
    15181 tgctctttca atttaaacgg cgactatcgg cttttatcct aatttatcgg ggctcctact
    15241 actaacacag taccctgtct gccggcaggc agacagcgtc gtgattggtc ttattccgat
    15301 ctatc
//
LOCUS       DAOPZK010000046        15272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_4187_length_15272_cov_22.769008, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000046 DAOPZK010000000
VERSION     DAOPZK010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4187_length_15272_cov_22.769008"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1150
                     /locus_tag="PKH68_04390"
     CDS             <1..>1150
                     /locus_tag="PKH68_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX12210.1"
                     /translation="LMKKISERTTLYYGLEETLNLVKSTVTNIDITTGANAAPAATRY
                     PDGGNTWNSAALYAQSNYVLSDIFSLTGGLRFTSVYMKSEFKNDRTAGNAIFPFDKAE
                     INEIAPCGNLALVANLPSKTKISATVSTGFRAPNIDDMVKFFDNTSSSTVVVPNTSLK
                     PEYAYNAELNIVQSLGRTGKIEAGAYYTLLDNALTVQPTQFNGKDSVVVDGTTLKALS
                     IQNSNSGRIYGYYIGANINPVKNLRLNGSLTATYGKYTNEQKEVVPMDHIPPMFGKIG
                     ADYSICKLKLEGFMRFSAAKQLADYSPSGEDNLSQTAITAKNAKGEATAWAGTAAWYT
                     LNFRAAYDVTKHINLNAGIENILDRHYRQFASGISAPGRNVYGTLRVSF"
     assembly_gap    1151..1250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1251..2933
                     /locus_tag="PKH68_04395"
     CDS             <1251..2933
                     /locus_tag="PKH68_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12211.1"
                     /translation="ATAQDIENIAKQKPFAITGGFGLNYTSTSSSDSNRIPMPVFWGA
                     NLSLNLSIYGISIPVTAVFTNGKVNLSHSFNQFGFSPHYKWITLHAGYRQYSYSPFTV
                     SGQTLFGGGMDVNYRKLRLGFFGGRLRKAVQVDSTMMFRQDIPGAYPVNISTENGINS
                     YSVRPTFSRIGLGGKIGFGTPTNFVDFILFKGYDNVKSLSTPTDNRDLKPEENVVLGV
                     NIFQRFLKHFTFGFNGAASIYTYDTNEELVKSDFQYLSVINKIIPVRLTTQAQWAAET
                     NINISYPNFNLSGSYKRAQPYFRSMGINSFLTDLNLISIQPSWSMLKQKLRFTNLLQY
                     QSDNLNRYKQLTTKRLMLNSSVSYQISDHWGIDLNYNGSDISQQKAKAQTADSIQSAQ
                     KSSSLTLSPRYLFSTDKISNITSLVTSFTGMRNSQIGGISNDIKNTYATLNNTFLIFR
                     GGWNINTGLNFNNAVTSQNTLQSYGFNAGVSKALFGNSLTLSNNNTLLFNTLDGVSNG
                     TTVSIDLNGNYNFLKRNTISIGYNYLYSPANGIYNLSDFQQNRLMISYQYNF"
     gene            2951..>6267
                     /locus_tag="PKH68_04400"
     CDS             2951..>6267
                     /locus_tag="PKH68_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12212.1"
                     /translation="MKTLNTFRNKLLIGLLFLSSGFLFSQQFSINLNLLPPYSPFYRD
                     YIGYTTNSTKSIVTLTYINPTNNVPAKVYLTASLRKDDGSISMEVKDDFRPGTPIALL
                     PNIPTTLTGTQLRNIFGNGTSNDLVMTGISTGDIITNQAMPEGTYNLCIKLKNYDTNT
                     LLAETCRSMFIAHSEPPQIITPLNNMSETEKTPQYVNVTWSQVTPYVQGITYRLRMVK
                     LLPGVSPYDAINYSTQVVLEKSNIKTTSFPLDLASGIKLDTGSVYVLQVTGITRSAYI
                     KNGGKSEPVQFMYKGMSVGNKRPPVIIKPGLAENIVFLNPEKPVNGQPDTLKVNNEND
                     LLLNWCWLKTKNKDTVTVADKEIIQQKDLKKYTINIERVKSLNKNNAVFAYNAEFLKI
                     DSTGMIKNFIQLTDSQAMAAGFVSNDIYRATLKAYDTKNKEVVSLVSPDFIFMKIADE
                     TPTYKIPLIATVNYGFKNNPEIFPVINSEVTVEAFKRQNSATAANITGADNSKLFNSL
                     PAENIGGINYVKITSAVFKTDSMGKISTVLAVPQKYFDTDSISYRIRLANSYYVDKDF
                     GIKTVPVSRKDSAVNLGYLTAKTYAYQLKLNVRKEFKSYRLVRDEKGLTVSLTDKDNL
                     FTNSSNQAVDNQQSYIYTAGEDTEGKDTIAEGIPVVLYRKYKQENIPVYEGNITKSGS
                     INKNKLSNITVVALGTTVKEKDSTYVTFNNLLSTNKIAEEYYILAVKNLKEFLNPYGV
                     EKQTGLKFSGSSNSQINDFSGLTVENSNLIANAIQQLTQGYSTTNFVDSGKFVAEEKP
                     FYLPLPQNTETDDAYYRTVKANYSITSCTPPTSLIKGRLFYVWKSDKNSVKRPLANTH
                     FRVMVDYVDDKNVSIGAVKNSQTPNFGMGGKWEYTAFVPNNSDQVIPLVDQYATMAEG
                     ITDDEGNFSIEVVNLNKKGDLGSGSVTHSSGSSKPPITGVSLEDEIKGKISGGDIVDP
                     INQFKDGFSNSQNSLNGNTGLSFDQTSQTFEVNGLKGNKAGNKAGGAAGGGIMFAPVA
                     EEIVHGPNPSSASPAGEPTPGNSTTTTNYNIFKRQFRIVIDGDKSDYYYPSREVIEIQ
                     PFESMTTPKDITHYVKE"
     assembly_gap    6268..6367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6368..>9417
                     /locus_tag="PKH68_04405"
     CDS             <6368..>9417
                     /locus_tag="PKH68_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12213.1"
                     /translation="LQSEYSNYLVQSSSTVNTAGKSFGEKTEKITEFTDNTTDWANPQ
                     IPVVSVSLKLTPLKSRVLALVRESGSNKPLTSDRNTRVILSKDESKPKPNYYNSKAVD
                     KYGYAEFLVDENQTKNFFIFKDDINSSDPVYFSASADGYTFGNQSSYKVVLNKKGTQN
                     APVLTLFPTATVTGKLLNADAFEYTPGAANVDNPVFVPSYIQVDSGKVYDINAVDKGI
                     FTIPIAPKAGVKIKIIPKDVAWFGDTLVLTKADETKQLIDLGNINLYRRKHRIQLNVF
                     TKTGIQNVIQNVRVKDARIKLGDWIVTTDQYGKAQFKFENVSINNYTFVINGPQGQGY
                     IPKTLNLKNEETRDFVSVDVILEKGSEVTGTVKLDGKPVKNARVYLEVQNSSASVAAV
                     NYAFQQNAQSINTNTNASQVAIQQPQGNSGNYQYNPNLTPSNSPTGEVTNDANLVVAY
                     TDASGKYTLKGVPVNNQKINVIATIDTTFTVSGDKQQADIKNGSAQLDLNLTSYSGAL
                     VNKLYGFPLTVEKITSINPSQIKVTGLVHWTEAISDFKLNEINKVLRVEDVLFDIKTA
                     ADNSGYAVVHENMVKIPGITELKLSYIDKYNVKLSTNNVPKSYNTKPLEITRDNDLGK
                     LDGYLQIEDNSFNYPSSYLDFKGSKFYLARLNNSKIDNKVSVATSAFTETESIDRSYN
                     DANKYLKGISTKISTYKQQPRPVYYLCDNKADSIEFKLINFTAKADPKKSYIDESGKI
                     HLNTTLSCHIANAQPENFRVVIPNIILDENKVDAASSASPIQLKLEKWTLEVREWTFS
                     TKEGGILSHNSLIKTDIIDIPVDTFVLRNDMFIMRANLKNLSLAGGKFPLTIASGKKP
                     QLNYEYKVGTDMMPHWNFSLLGSGDTKVAMLPKLKGLPDDYRIDLNYIEILSNNEMIV
                     QMMQKSDTLLFGNKVARFEPLTLFNGPNYINVSGQLHTGAPRMSNILLTAKWSDPNSI
                     PTFEKVSTDFEAKGFVHFEAKKKKITIDNNLVTIEGRVLEKPDLT"
     assembly_gap    9418..9517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9518..10132
                     /locus_tag="PKH68_04410"
     CDS             <9518..10132
                     /locus_tag="PKH68_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12214.1"
                     /translation="MGILAGVYNLGMMAGYHKVTDELWGVTNASMNPAVVNNCYRAQL
                     PNNQLSGFYFTASREIINFEESFGFILADGYVRATALLGGSFYVNYQPGNFLTGAEGF
                     VHADVSAGLSAITGTSISGSVKGDGRIGFVFSDPSYFYTSTQLGFEAKIEQDLGVKTI
                     SASKSVNCVLSAKAGTGGSGFDFSFGSSGDNPDCTIKKENNTTN"
     gene            10145..12136
                     /locus_tag="PKH68_04415"
     CDS             10145..12136
                     /locus_tag="PKH68_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12215.1"
                     /translation="MKSIIIRKTGILLAMMLTLHITATGSESKAYQTGIFVFCGKEIP
                     KSFSYIIEKKTGTNTWKPVAELKAPESEAACKASLMQLPAVIEAVTPVSQSTIAFVWE
                     KIKKSVTVDSLHIYSTDPRYQYVAGTGWFDDGIRESGNYSYRIKRLNKNGTLSQISEI
                     SVPFPAKQPELKAAPVRFKLNENSISISYEVSNPALTSGIELYRSAYLTNNFQKVPAK
                     LLYTRQKDKMVAVLTDETATKGLTYSYRAQPYDALANKGFMSDTLNIYFVAKAADIGL
                     ITKFQAFPQPEKAGNLLQWEFDYRMNVNTIDIYRCEEYDGKYELIASVSPKTREYFDG
                     RNITPSRVNYYYIAINTGVGSSLPSPRVPAILEGKKPNNIPPQDLVATRTGNVVTLKF
                     RSVDSDTRAFYIYRGDGYTAPLEQLPRMLLSDSAELTYNDTLPSTMNAAVYSYAVASV
                     NSSYNISPKSKRANVTYSGGHLPVPNDVNAMMSNNAVLLTWSDAAKTHSGVTGYRIFR
                     KAEYNNQIETPEQVIAETGFSNNSLIDSLVIPARYYTYRVQCMGEDTADTGSLSQQAG
                     ILYKGDRLLQPGNVTAIPSDKKIILKWTLPVADNLLSALIYRSVENGEPILLKETDAK
                     TEFFEDTAAASGTMYYYFIVLKYKHNLTSTPTDAVGAKW"
     gene            12361..12627
                     /locus_tag="PKH68_04420"
     CDS             12361..12627
                     /locus_tag="PKH68_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNX12216.1"
                     /translation="MKELTELPNIGKVLAQKLTEVDIKSPGDLISAGSENAFIRIKTV
                     DPDACINMLYALEGAIQGIRWHNLTAERKRELTAFFNHFRTLQT"
     gene            12624..12992
                     /locus_tag="PKH68_04425"
     CDS             12624..12992
                     /locus_tag="PKH68_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNX12217.1"
                     /translation="MNEVDEYITGFPPEVQQIMHEIRRIITDAAPDAVEGMAYGMPGY
                     KTHSKPLVYFAGYKKHIGFYATPTGHSAFAEELAQYKQGKGSVQFPLDKPIPYDLIHR
                     IIVFRALENSVISNSKPKKS"
     gene            13199..14416
                     /locus_tag="PKH68_04430"
     CDS             13199..14416
                     /locus_tag="PKH68_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNX12218.1"
                     /translation="MFLNTEIAKDIFYVGVNDRIKHKFENMLPLPYGVSYNSYLITDE
                     KTALIDTVDVCYGEVFIDKIKSQLGEKSIDYLIINHMEPDHSGSISLIRKYYPNMTIV
                     GNKKTLEMLEGYYGIDDNTLEISDGEVLSLGKHKLQFHFTPMVHWPETMMTYDETENV
                     LFSGDAFGCFGTLDGGVMDTEMNVDKYWDEMIRYYANIVGKYGAPVQKALQKLTPLDI
                     KIICSTHGPVWKEYVSKAVDIYDRMSRYEGKEGAVVVYGSMYGNTERMAEAVAQGLTM
                     GGIKNVILHNVSKSDASVILRDIFKYKGLVLGSPTYSNELYPEIDSLLKKMEIRGIKK
                     RDFAYFGSFTWAGIANKKLAEFGEKMKWRTINFTVEEKMGLKDSNYQSCILLGRKTAE
                     LLKTPEPVDPSCH"
     gene            14730..15125
                     /locus_tag="PKH68_04435"
     CDS             14730..15125
                     /locus_tag="PKH68_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNX12219.1"
                     /translation="MEELTLITPTFLFSAVSLILLAYTNRFLAYTSLVRNLKVKYMEN
                     KSSVTKAEIENLKKRLKLTRLMQLYGVASLLLCVATMFLIYIGLQTVSAVIFAVALLF
                     LIISLALSVWEINISSKSIQIHLSDMEAK"
BASE COUNT         4802 a         3272 c         3096 g         3802 t
ORIGIN      
        1 ctgatgaaaa aaatcagtga acggacaacg ttgtattacg gactggagga aacactgaac
       61 ctggtaaagt cgaccgtaac gaacatcgac attaccaccg gagccaatgc agcacctgcc
      121 gccacccgat atcccgacgg gggaaatacc tggaattcag ccgcgttgta cgcgcagagc
      181 aactacgtac tgtccgatat tttttcgtta acgggtggtc tgcggtttac ttccgtgtac
      241 atgaagtcgg aatttaagaa tgaccgtacg gccgggaatg ctattttccc tttcgacaag
      301 gcggaaatca acgaaatagc accctgtggt aacctggcac tggtagccaa tctgccttcg
      361 aaaacaaaaa tcagcgccac ggtttccacc ggatttcgcg cgccaaacat cgacgatatg
      421 gttaaattct tcgacaacac aagcagttca acggttgtgg tgccgaatac aagtctgaag
      481 cccgaatatg cttacaatgc tgaattgaat atcgttcagt cgctgggacg taccgggaaa
      541 attgaagcag gagcttacta caccctgctt gataacgccc tcaccgtaca accaactcag
      601 ttcaatggta aggattcggt cgtagttgac ggaaccaccc tgaaagctct gtccatacag
      661 aactccaaca gcggacgaat ctatggttat tatatcggtg caaatatcaa tccggttaaa
      721 aacctgagac tgaacggttc gcttactgcc acttacggaa aatacaccaa tgagcagaaa
      781 gaagttgttc cgatggatca tattccaccc atgtttggaa aaatcggagc tgattacagc
      841 atctgcaagc tcaaactcga agggtttatg cgcttcagtg ccgccaaaca actggctgat
      901 tacagcccga gcggggaaga caacctcagc cagacagcca tcacagccaa aaacgcgaaa
      961 ggtgaagcta cagcctgggc gggtactgct gcctggtaca ccctcaactt ccgtgctgct
     1021 tacgacgtga ctaagcatat taatctgaat gcaggaatcg agaatatact cgaccggcat
     1081 taccgtcaat ttgcatcggg tatcagcgca ccggggcgga atgtgtatgg caccttgagg
     1141 gtaagttttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccaccgccc
     1261 aggatattga aaacattgcg aagcaaaaac catttgcaat taccggcggg tttggtctca
     1321 actacacttc caccagttcc agcgacagca accgcattcc catgcctgta ttctggggtg
     1381 ccaacctcag tctcaatctg agtatttatg ggataagcat tccggtgaca gccgtgttca
     1441 ccaatggaaa agtcaatctc agccattcgt tcaatcagtt tggtttcagt ccgcattata
     1501 agtggataac gctgcatgca ggctaccggc aatactccta ctcgccgttt acggtatccg
     1561 gtcagacttt gtttggtgga ggcatggatg taaattacag gaaactacgg cttggatttt
     1621 ttggcggcag gctgcgcaaa gcggttcagg tcgactcgac catgatgttc cgccaggata
     1681 ttccgggagc ttatccggtg aacattagca ccgaaaacgg gatcaactcc tactccgttc
     1741 gtcccacctt ttcgcgcatt ggtttgggtg gaaaaatagg tttcgggaca cccaccaatt
     1801 ttgtggactt catattattc aagggatacg acaacgtgaa aagtctcagc accccgactg
     1861 ataaccggga cctgaagccc gaagaaaatg tggtattggg agtgaatatc tttcaacgat
     1921 ttctgaaaca cttcactttc gggtttaacg gagctgcaag catttacacc tacgacacga
     1981 atgaagaatt ggttaaatca gattttcaat acctcagtgt gattaataaa atcatacccg
     2041 taaggctaac cactcaggct cagtgggctg cagaaaccaa catcaatatc agttacccca
     2101 atttcaacct gtcggggtca tacaaaagag ctcaacccta cttcagaagc atgggtatca
     2161 attcatttct gaccgacctg aacctgatat caattcagcc atcgtggagt atgctgaaac
     2221 agaaattaag atttacaaac ctgctccagt accaatccga caatctgaac cgatacaaac
     2281 aactgaccac caaacgcctc atgctgaatt catcggtgtc gtatcagatt tcggaccact
     2341 ggggcattga cctcaactac aacggcagtg acataagcca gcagaaagca aaagcacaga
     2401 ctgcagactc catccagtcg gcacaaaaaa gcagttcgtt gaccttgtcg ccccgctacc
     2461 tgttcagcac agataaaata tccaacatca catcattagt aacaagtttc accggtatgc
     2521 gcaacagtca aatcggaggt atttccaacg acattaaaaa cacctacgcc actctcaaca
     2581 atacattttt aattttcagg ggcggatgga atattaatac cggactgaat ttcaacaatg
     2641 ctgtcacatc gcagaacaca cttcaaagct atggcttcaa tgccggtgtg agcaaggccc
     2701 tttttggcaa ttcattgact ctgtcgaaca acaataccct gttgttcaac acactggatg
     2761 gggtaagcaa tggcaccact gtttccatag atttgaacgg aaattacaat ttcctgaaaa
     2821 ggaataccat aagtatcggt tataactacc tgtattcccc tgccaacggc atttacaatt
     2881 tgagtgattt tcagcagaac aggctgatga tttcgtatca atataatttt taataaagac
     2941 accccaaccg atgaaaacct taaacacctt tcgtaacaag ctgctgattg gacttctgtt
     3001 cctcagctca ggttttttgt tttctcagca gttcagtatc aacctaaatc tgctgcctcc
     3061 ctattcccct ttttatcgcg attatatcgg atataccacc aatagtacaa agtctattgt
     3121 gaccctcacg tatatcaatc cgacaaataa tgtacccgcc aaagtgtatc tgaccgcttc
     3181 actgcgcaaa gacgacggaa gtatctcgat ggaggtgaaa gatgattttc gaccgggaac
     3241 gcccatcgct ttactgccca acataccgac aacgctcaca ggcactcaac ttcggaatat
     3301 tttcggcaac ggtacatcca acgatctggt gatgacgggc atcagcaccg gtgatatcat
     3361 caccaatcag gccatgcccg aaggcactta taatctttgt atcaaactga aaaattacga
     3421 cacgaataca ttgcttgccg aaacctgtcg ttcgatgttc attgctcaca gtgagcctcc
     3481 acaaattatt acaccgctca acaacatgag tgaaactgaa aaaacgcccc agtatgtaaa
     3541 tgttacgtgg tcgcaggtta cgccttatgt tcagggaatt acgtatcggc tcagaatggt
     3601 caaactgctt ccgggagtta gtccctacga tgctatcaac tactcgacac aggtggtact
     3661 cgaaaaatcg aatatcaaaa caacaagttt cccattggat ttggccagcg gtataaaact
     3721 cgacaccggc tctgtgtatg tgctgcaggt tacaggtatc acacgaagtg catacattaa
     3781 aaatggcggg aaaagtgaac ccgttcagtt tatgtataag ggaatgtccg tcggaaataa
     3841 gagacctcct gtaatcataa aacccggcct ggcagagaat atagtttttc taaatcccga
     3901 aaaacctgta aacgggcaac ccgacaccct gaaagtgaac aatgaaaatg atctgttgct
     3961 gaactggtgc tggctgaaaa ccaaaaataa agacacggtt accgtagccg ataaggaaat
     4021 tattcagcaa aaggatttga aaaaatacac catcaacatc gaacgggtga aaagtctgaa
     4081 caaaaacaat gcagtttttg cctataatgc tgaattcctg aaaattgaca gtaccggaat
     4141 gattaaaaac ttcattcagc ttaccgattc gcaggcgatg gctgccggct ttgtaagcaa
     4201 tgatatctat cgggcaacgc ttaaagccta tgacacgaaa aacaaagagg tggtttcgct
     4261 ggtaagtccc gattttattt tcatgaaaat tgccgacgaa acgccaacgt ataaaattcc
     4321 cttgatagcc acagtaaatt acgggtttaa aaataatcct gaaatttttc cggttatcaa
     4381 ttcagaagtg accgtagaag cattcaaaag gcaaaattct gccacagcgg caaatattac
     4441 aggtgctgat aacagtaaat tatttaacag cctgccggcg gaaaatattg gcggaataaa
     4501 ttatgtaaaa atcacttctg ctgttttcaa aaccgacagt atgggaaaaa taagcaccgt
     4561 tctggctgtt ccccaaaaat acttcgacac cgattctatc agctaccgca tcaggttagc
     4621 caacagttac tacgtggata aggattttgg aattaaaacc gtgccggtaa gcaggaaaga
     4681 ttcagcagtg aacctcggat atctgactgc aaaaacgtat gcctatcagc tgaaactgaa
     4741 tgtacggaag gagtttaaat cctacaggct ggtcagggat gaaaagggat taactgtttc
     4801 attaaccgat aaagataatt tatttacaaa ctcgagcaat caggctgttg ataatcaaca
     4861 atcgtacata tatacagcag gagaagacac tgaaggaaaa gacaccatag ccgaaggaat
     4921 accggtggta ctttacagaa aatacaagca ggaaaacata ccggtttacg aaggtaatat
     4981 tacaaaatcg ggcagtatca ataaaaataa actgtcgaat atcacagtgg tggcactcgg
     5041 tacaaccgtt aaagaaaagg acagcaccta cgtaacgttc aataatttgc tatcgaccaa
     5101 taagatagca gaagagtatt atatattagc cgtcaaaaat ctcaaagagt ttcttaatcc
     5161 atatggtgta gaaaaacaaa ccggattaaa attttcagga agcagcaatt ctcaaatcaa
     5221 tgatttttcc ggtctgacgg tggaaaacag caatttaatt gccaatgcca ttcaacaatt
     5281 aacgcaagga tattcaacaa cgaactttgt cgattctggt aaatttgtag ccgaagaaaa
     5341 accattttat ctgccactgc cacaaaatac tgaaactgat gatgcttatt atcgaacagt
     5401 gaaggcaaat tattccatca cctcctgcac tccacctaca tcgctcatta agggccggtt
     5461 gttttatgtc tggaaatcgg acaaaaacag tgtgaaacga ccgttggcca atacccattt
     5521 tcgggtcatg gtcgattatg tagatgataa aaatgtatca atcggagctg taaaaaactc
     5581 acaaacacct aatttcggta tgggtggaaa gtgggaatat actgcatttg ttccgaataa
     5641 ttccgatcag gttataccac tggtcgatca gtatgccacc atggccgaag ggattactga
     5701 tgacgaaggg aatttctcga tagaagttgt caatttgaat aaaaaaggtg atttgggttc
     5761 cggatcggtg acacacagct cgggaagcag caaaccgccc attacaggag tatcgcttga
     5821 agatgaaata aaaggaaaaa tatccggtgg tgatatcgtt gatccgatta atcagtttaa
     5881 agatggcttt tccaacagtc agaattcact aaatggaaat accgggctaa gtttcgatca
     5941 aacatcacaa accttcgaag tgaatggact aaaagggaac aaagcgggca ataaagccgg
     6001 tggagcagcc ggtggaggca tcatgtttgc tccggtggct gaggagatag ttcacggccc
     6061 caatccatca tcagcctcac cggcagggga accaactccc gggaattcca ccacaacgac
     6121 aaattataat attttcaagc ggcagttccg gattgttatt gatggcgata aatcggatta
     6181 ttactatcca tcgagggaag ttattgaaat tcagcctttc gaaagcatga ccacacctaa
     6241 agatattacc cattatgtga aagaattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnctc cagtcggaat acagtaacta cctggttcaa tcgtcgagta ctgtaaacac
     6421 cgccggaaaa tcgtttggtg aaaaaacaga aaaaatcact gaatttactg ataacaccac
     6481 cgattgggct aatccgcaaa taccggttgt gagtgtttct ttaaaattaa ctccgctgaa
     6541 aagcagggtt ttggcactgg tgcgcgaatc gggcagtaac aaaccgttaa catccgacag
     6601 aaacacacgc gtaattttat cgaaagacga atcgaaacct aaacccaatt attacaattc
     6661 taaggcagtt gataaatatg gttatgccga atttctggta gatgaaaatc aaaccaaaaa
     6721 ttttttcata tttaaagacg atataaattc ttctgatccg gtttactttt cggcatcagc
     6781 cgatggttat actttcggaa atcagtcatc gtataaagta gttttgaata aaaaaggcac
     6841 tcaaaacgcc ccggttctta cacttttccc aactgccaca gttacgggga aattgctcaa
     6901 tgccgatgca tttgaataca ctccgggtgc tgccaacgta gataatccgg tttttgtacc
     6961 ttcgtatatt caggttgact ccggaaaagt gtatgatata aatgctgtgg acaaaggcat
     7021 atttacaatc ccgatagcgc caaaagccgg tgtgaaaatc aaaatcatcc cgaaggatgt
     7081 agcctggttt ggcgacacgc tcgtacttac caaagccgac gaaacaaagc agcttatcga
     7141 cctggggaat attaatcttt accgccgaaa acaccgcata caattgaatg tgtttaccaa
     7201 aaccggcata cagaatgtta ttcaaaacgt aagagtgaaa gatgccagaa tcaaactcgg
     7261 agactggatt gttactaccg atcaatatgg taaagcacaa ttcaaatttg aaaatgtatc
     7321 aatcaacaat tatacatttg tgattaacgg accgcagggt cagggttaca ttccaaaaac
     7381 tctgaatttg aaaaatgagg aaacgcgcga ttttgtatcc gttgatgtta ttcttgaaaa
     7441 aggaagcgaa gtaaccggca cagtgaaact cgacggaaaa ccggtgaaaa acgcccgtgt
     7501 ttatctggaa gttcagaaca gctccgcttc ggttgctgcg gtaaattatg cttttcagca
     7561 aaatgctcaa tccattaata caaacacaaa tgcctcacaa gttgcaatcc aacaaccgca
     7621 gggcaatagc ggaaattatc agtacaatcc taatcttact ccttctaatt ctcccacagg
     7681 tgaagttacc aacgatgcca acctggtagt ggcatatacc gatgcttcgg gtaaatatac
     7741 gttgaaaggt gttccggtta acaatcagaa aatcaatgta attgcaacca tcgacaccac
     7801 atttaccgta tcgggcgaca aacaacaggc tgatatcaaa aacggcagcg cacagttgga
     7861 tttaaatctc acttcataca gtggtgcact ggtcaataaa ctgtatgggt tcccgctaac
     7921 ggtcgaaaaa attacttcca taaatccctc gcaaattaag gttacagggt tggtacactg
     7981 gaccgaagcc atcagcgact ttaaactgaa tgaaattaac aaggtacttc gtgtcgaaga
     8041 tgtattgttt gatattaaaa ccgctgccga taattcaggt tatgcagtag ttcacgaaaa
     8101 catggtgaaa atacccggaa taactgaact gaaattatcg tacatcgata aatacaacgt
     8161 aaaattatcg accaataatg tgcccaaatc ctataatacc aaaccgctgg agattacccg
     8221 cgataatgat ttgggaaaac tcgacggtta tttgcaaatt gaggataact cgttcaatta
     8281 cccgagcagt tatctcgatt ttaagggaag taagttttat ctggcacgcc tgaacaattc
     8341 gaaaatagat aataaagtga gtgtagccac aagcgccttc accgaaaccg aatcgatcga
     8401 cagatcgtac aacgacgcca ataaatacct taagggaatc agcacaaaaa tttcgactta
     8461 caagcaacag ccaagaccgg tgtattactt atgcgataat aaagcagatt ccattgaatt
     8521 taagctgatt aactttactg ccaaagccga cccgaagaag agctatatcg acgaaagcgg
     8581 aaaaatacac ttgaatacta cgcttagctg tcatattgcc aatgcgcaac ccgagaattt
     8641 cagggtggta atacccaata tcatacttga tgagaacaag gttgatgcag cttcatcagc
     8701 ttcacccatt cagttaaaac tcgagaagtg gacactcgaa gtgcgcgaat ggacgttcag
     8761 taccaaagaa ggcggcattt taagtcacaa cagtttgatc aaaacagata ttattgatat
     8821 tccggtagat acgttcgtac tccgcaacga tatgtttatc atgagagcaa atttgaaaaa
     8881 tctcagttta gccggtggaa aattcccgtt aaccatagca agcggtaaaa aacctcaact
     8941 caattacgaa tataaagtgg gaaccgacat gatgccacac tggaatttca gtctcctggg
     9001 atcaggcgac acaaaagtgg caatgctgcc taaactgaaa ggattgcctg acgattatag
     9061 gattgatttg aattatatcg aaattttgag caacaacgaa atgatagtgc aaatgatgca
     9121 aaaatcagac accttgctct ttggcaataa agtggcacga ttcgaaccgc ttaccttatt
     9181 caacggacca aattatatca atgtatcagg ccaattacat accggagcgc cccgcatgag
     9241 caatattcta ttaaccgcca agtggtctga tccaaattcg atcccaacgt ttgaaaaagt
     9301 ttccaccgat tttgaagcaa aaggatttgt ccatttcgaa gctaagaaga aaaaaatcac
     9361 cattgacaat aacctggtta ccatcgaagg acgcgtgctg gaaaaacccg acctgacnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg ggaattcttg caggtgtata
     9541 taatttaggc atgatggcgg gctatcataa ggttaccgac gaactgtggg gagttaccaa
     9601 tgccagtatg aacccggcag tggtaaacaa ctgttacaga gcccaacttc caaataatca
     9661 attgtcaggt ttttatttta ccgccagtag ggaaataatt aacttcgaag aatcgttcgg
     9721 atttatactt gccgatggct acgttcgggc tacagcatta ttgggtggca gtttttatgt
     9781 caattaccag cccggaaatt tcctaaccgg agccgaaggg tttgtacatg ctgatgtaag
     9841 cgccggactc tcggcaatta ccggtacaag tatatccggc agtgtaaaag gtgatggaag
     9901 aattggcttc gtgtttagcg atcccagtta tttctataca agcactcaac ttggattcga
     9961 agcaaaaatc gaacaggatt tgggagtgaa aactatttcg gcttctaaat cggttaactg
    10021 tgttttgtca gctaaagcag gtaccggagg aagcggtttt gatttttcgt ttggaagtag
    10081 tggcgacaat cctgattgta caataaaaaa agaaaacaat acaaccaact aaatcattta
    10141 tcgaatgaaa tcaataataa tcaggaaaac cggtattttg ctggctatga tgctgacatt
    10201 acatataaca gctacaggaa gtgaaagcaa agcctatcaa accggtatat tcgttttttg
    10261 cggaaaagaa attcccaaat ctttctctta cattatcgag aaaaaaaccg gcaccaacac
    10321 ctggaaaccg gtggctgaac tcaaagcgcc tgaaagtgaa gcagcatgta aagcatcact
    10381 tatgcagctt ccggctgtta ttgaagctgt tacgcccgtc agtcagtcaa cgatagcttt
    10441 cgtgtgggaa aaaattaaaa aatcggtcac cgttgattcg cttcatattt acagcaccga
    10501 tccgcgttat cagtacgtag ccggcaccgg ttggtttgac gatggaattc gtgaatcggg
    10561 caattacagt taccgcatca aacgattgaa taaaaatggc actttgtcgc aaatcagtga
    10621 aatttcagta ccctttccgg caaaacagcc tgaactgaaa gcagcacctg taaggttcaa
    10681 actgaacgaa aattcaatca gcataagtta cgaggtttcc aatccggcac tgacaagcgg
    10741 cattgaactc taccgctcgg cttatctgac caataatttt cagaaagtac cggcaaaact
    10801 tctttacaca aggcaaaaag acaaaatggt ggctgtattg actgatgaaa ctgcaacaaa
    10861 aggcttaacc tacagctatc gcgcccagcc ctacgatgcc ctggccaaca aaggattcat
    10921 gtccgacacg ctgaacattt attttgtagc caaagcagca gatattggtt tgattaccaa
    10981 atttcaggct ttccctcagc ccgaaaaagc cggaaatctg cttcaatggg aatttgacta
    11041 ccgaatgaat gtgaacacga tagatattta ccgctgcgaa gagtacgacg ggaaatacga
    11101 attaattgcc tcggtcagtc ccaaaaccag agaatatttc gacggcagaa acataacgcc
    11161 atccagggta aattactact atattgcaat caacaccggc gttggcagca gtttgcccag
    11221 tccccgcgta ccggcaattc tcgaagggaa aaagcccaac aacattccgc ctcaggattt
    11281 ggtggcaacc cgcacaggta atgtggtaac gcttaaattc cgaagtgtcg acagcgatac
    11341 ccgtgcattt tacatctatc gcggcgatgg atatacagca cctctggaac aactgccacg
    11401 catgttgctt tcggattccg cagagcttac ttataatgac acgctgccct caaccatgaa
    11461 tgcggccgtt tacagttatg cggttgcttc ggtcaattca tcgtataata tcagtccgaa
    11521 aagtaaacgg gcaaatgtta cttacagtgg tgggcatctg ccggttccaa acgatgtgaa
    11581 tgcgatgatg agcaacaatg cggtgttact gacctggtcc gatgcagcta aaacacattc
    11641 aggagtaacc ggttacagga ttttcagaaa agccgaatac aacaatcaga ttgaaactcc
    11701 cgaacaggtg attgctgaaa caggattttc aaacaattca ttgatcgatt cactggttat
    11761 acctgcaaga tattatacgt accgtgttca gtgcatgggt gaagacactg ccgataccgg
    11821 aagtttaagc caacaagcag gaattctcta caaaggagat cggttactgc agcccggtaa
    11881 tgtaactgcc attccgtccg ataaaaaaat aatactgaaa tggactttac ccgttgccga
    11941 taatttgctt tcagcgctga tttatcggtc ggttgaaaac ggcgaaccaa ttttgcttaa
    12001 agaaactgat gcaaaaaccg aatttttcga agatactgca gctgcatcgg gcaccatgta
    12061 ttattatttt attgtgctga aatacaagca taatctcacg tccactccta ccgatgcagt
    12121 tggagcgaaa tggtgaaaat tcattctttt tgaatgaagt catcttaact ttttattaat
    12181 acagataatt tatcgtttta aaccacgaat ttccctaatt acacgaataa attaggagtt
    12241 aaaattaaaa agtcaagata aaaccaataa caataaaaca taattttgtg gataaaagta
    12301 aaataaaatc tggacgaagg agtaacaatg aatatcacgg atttagcaat cattttgtca
    12361 atgaaagaac ttaccgaact gccaaacata ggcaaagtac tggcgcaaaa actgacagaa
    12421 gtggatataa aatcgcccgg ggatttaatt tcagccggga gtgaaaatgc atttatccgt
    12481 attaaaactg ttgatcccga tgcctgcatc aatatgcttt acgcgctgga aggtgctatt
    12541 cagggtatta gatggcacaa cctgaccgca gagcgaaaaa gggaacttac cgcattcttt
    12601 aatcattttc gtaccctaca aacatgaatg aagtagatga atatatcacg ggctttcctc
    12661 ctgaagtgca gcagataatg catgaaatcc gccggataat aaccgatgca gctcccgatg
    12721 ctgtggaagg gatggcttac ggaatgcccg gctataaaac tcacagtaaa ccgctggtgt
    12781 attttgcggg atacaaaaaa cacatcggtt tttatgccac tcccaccggt catagtgctt
    12841 ttgccgaaga actggcacag tataaacagg gcaaagggtc ggttcagttt ccgctcgaca
    12901 aacccattcc ttacgacctc atacaccgga taattgtgtt cagggcactc gaaaattccg
    12961 taatatccaa ctctaaacca aagaagtctt aaacctgttt ccggttgcaa ggcggatgtt
    13021 cctatatttt ttcactcata tggaattccc tttaatcctt tcctaaaata atttgtatct
    13081 ttgtattccc aaaaaacaat cgttccagtc aattgttttt tcaggaaaaa gattgactat
    13141 tttatcaccc attcccgatt ataaaccgga tctatcggga atattatatg tttgaactat
    13201 gtttctgaat actgaaattg caaaggatat attttatgtt ggagtcaatg accgcatcaa
    13261 gcataaattc gagaatatgc tcccgctgcc ttatggcgtg tcttacaatt cctacctcat
    13321 aacagatgaa aaaactgcac tcattgatac cgttgatgta tgttacggtg aagtgtttat
    13381 tgacaaaata aaatcacaat taggcgaaaa atccattgat tatttgataa ttaaccacat
    13441 ggagcccgac cactccggtt ctatcagcct tatccgaaaa tactacccca acatgaccat
    13501 tgtgggcaat aaaaaaaccc tggaaatgct cgaaggttac tacggaatag acgacaatac
    13561 gctggaaatc agcgatggtg aagttctttc gcttggcaaa cacaagcttc agtttcactt
    13621 cacacccatg gttcactggc ccgaaacgat gatgacctac gatgaaaccg aaaatgtact
    13681 cttctcgggc gatgctttcg gatgcttcgg tacactcgac ggcggcgtga tggatacgga
    13741 gatgaatgtg gataaatact gggatgaaat gatccggtac tatgccaata ttgtgggaaa
    13801 atacggagct ccggtacaga aagcactgca aaaactcact ccgctcgata tcaaaattat
    13861 ctgttcgacc cacggcccgg tgtggaaaga gtatgtatca aaggcagttg acatctatga
    13921 ccgtatgagc cgctacgagg gcaaagaagg tgctgtggtg gtgtatggtt ccatgtatgg
    13981 caataccgag cgcatggccg aagcagtggc acagggactg acgatgggtg gcatcaaaaa
    14041 cgtaatactc cacaatgtgt ccaaatcgga tgcgtcggtt attctgcgcg acatattcaa
    14101 gtacaaggga ctggtactag gcagccctac gtatagcaac gagctctatc ccgaaatcga
    14161 cagtttgctg aaaaaaatgg aaatcagagg tatcaaaaaa cgtgattttg cttactttgg
    14221 ttcatttacc tgggcaggca ttgctaataa aaaactggct gaattcggtg aaaagatgaa
    14281 atggagaacg attaatttca ccgtggaaga aaaaatgggt ttgaaggaca gtaattatca
    14341 atcctgcatc ctgcttggca ggaaaacggc cgaactgctc aaaacacccg aaccggttga
    14401 cccaagctgc cattaaaagc ttttgctttc gtttttcaac ttggatttga atgaattggt
    14461 aagaattcct gaatgtacta aagcctaaat aaacctgaga tcgatgaaat agaagaatat
    14521 ttaaatgctg ataaaattct gaccagcaat aaggtaataa ggttttcttt attttagcaa
    14581 tttctactaa ggttttcatt cttttttata aaaccttatt ttcaaaataa ttaaccttag
    14641 tagaactgta attaccctga accctaacct tatttacaga tttttataaa atttgttatg
    14701 tcgaactcac attaaaatac aagaccgcca tggaagaatt aacgctcatc actcccacct
    14761 ttctgttttc ggcggtttcg cttattctgc tggcctacac caaccgcttt ctggcgtaca
    14821 catcgctggt tcggaatttg aaagttaaat acatggagaa taaatccagt gtaaccaaag
    14881 cggaaattga aaacctgaaa aaacggttga aactgacccg cctgatgcaa ttgtacgggg
    14941 tggcgagttt gctgctctgt gttgcaacta tgttcctcat ttacatcgga ttacaaactg
    15001 tttcagccgt cattttcgca gtagccctgt tgtttctcat catttcactg gctttgtcag
    15061 tctgggaaat caacatctcg tccaagtcta ttcagataca tttgagcgat atggaggcaa
    15121 aataaatctt cgactagctg ccccctaacc ccctaaaggg ggaatattgg tgcaaaaatt
    15181 caatattcaa aaaaaaaaca agcactcaaa aaataatacc acttataaaa accacaaatg
    15241 atggaaaaac caactacaaa ttcccccttc ag
//
LOCUS       DAOPZK010000047        15267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_4189_length_15267_cov_23.089469, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000047 DAOPZK010000000
VERSION     DAOPZK010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4189_length_15267_cov_23.089469"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3510
                     /gene="gltB"
                     /locus_tag="PKH68_04440"
     CDS             <1..3510
                     /gene="gltB"
                     /locus_tag="PKH68_04440"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNX12220.1"
                     /translation="YHSMLMEPWDGPATLLFSDGRFAGGMLDRNGLRPARYTITNDDR
                     MIIASETGTIELEASNIKARGRLRPGKMLIVDTKTGQIFYDNELKDGLAKAFPYRDWL
                     NKNCSNLDVISSGRSVNNDISNYETLMTAFGYSVEDIEKLILPMADEGKEPTSSMGND
                     VTLAAFSNKPQRLFNYFRQQFAQVTNPPIDPIREELVMSLTGYVGSVHQNLLEQIPEL
                     SKIVKLKSPILTNTQFDILLNLRYKGFSTAVLPMLFDPKGGTRGLEKAIDELCITVEK
                     AVDEGKNYIVLSDRGVDAAHAPIPSLLAVSAVHLYLVEKRKRMQIDIVVESAEPREVM
                     HFALLFGFGANAVNPYLAFAVLNRKVKSEDIQLDFDTAKKNYIKAINKGLLKVLSKMG
                     NSTLRSYRGAHIFEAIGISSRVLNAYFKDISSKIEGIDMEDITREVLQPFYEAFAADN
                     NEPFHLKNLGHYAYRNDGEYHAWNPETIARLQIATKTGDYSKYKEYSALVDNKSNPAF
                     IRDLLDYKRNPIDISEVEPAENIMKRFCTGAMSYGSISREAHEAMAIAMNKIGGRSNT
                     GEGGEDPERYKKRDDGLSTRSAIKQVASGRFGVTAEYLVNADELQIKIAQGAKPGEGG
                     QLPGYKVDKIIAKTRHSIPGISLISPPPHHDIYSIEDLAQLIFDLKNINPKAIVSVKL
                     VSESGVGTIAAGVAKAKADLILISGAEGGTGASPSSSIKHAGLPVEIGLAETQQTLVM
                     NNLRGKIRLQTDGQLKTGHDIIISTLLGAEEFGFATSALIVLGCVMMRKCHLNTCPVG
                     VATQNEELRKRFVGRYEYLVNFFTFLAEETREHLAQLGFKTLDEAVGRADLLERKQLP
                     DYPKTEKIDLSRITFMPKEAAVNALRKTSDQDHKLDSILDRELISKSLPALDLCMPVT
                     IQSKIKNTDRTTGAMLSGEIAKRYGQAGLPENTISIQFEGSAGQSFGAFLSKGMTFHL
                     QGDANDYLGKGLSGGRIIVTPPKGSTFAPEENILCGNTLLYGATSGEVFINGVVGERF
                     CVRNSGATAVVEGAGDHCCEYMTGGRTVVLGITGRNFAAGMSGGVAYVLDEKGDFDYF
                     CNMEMVELSLIEESGDNKELHGLIEAHYKYTDSPLAKKILDSWNEYVDKFIKVVPIEY
                     KKVLEEEKIEAIKRKIASVERDY"
     gene            3635..5065
                     /locus_tag="PKH68_04445"
     CDS             3635..5065
                     /locus_tag="PKH68_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNX12221.1"
                     /translation="MGNPKAFISIPRKEAGYRPVLERIYDFGEVEQTLNQEDRKLQAS
                     RCMDCGIPFCHWACPLGNKMPEWQDLIYKGRWKEAIEVLHETNNFPDFTGRICPAPCE
                     KSCVLNLSEAPVTIRENEAAVTEVAFIEGMIKARPPKTRTGKKVAVIGSGPAGLAAAQ
                     QLNRKGHTVTVFEKDNSIGGLLRYGIPNFKLNKNIIDRRLAQLVEEGIIFKPNTEIGK
                     TISGTEIIKKFDAVCLAVGAGHPRDISPAGRELKGIYFAMDFLAQQNQVIMEEKLTKP
                     ERITAKDKHVLVIGGGDTGSDCVGTSLRQGAVKVTQIEILPKPPAGKNPETPWPYYPN
                     ILKTSSSHLEGCERKWSLNTVEFKGNNGQLEEVVVEKVEWKKDESGRFTMISTGKTEI
                     IKADLVFLALGFVHPVHEGLLTELGVNLDARGNVAVDRQNQSSVAKVFAAGDAAVGAS
                     LVVRAIASGRNVAEDIHKKLGGPPTP"
     gene            5247..6920
                     /gene="asnB"
                     /locus_tag="PKH68_04450"
     CDS             5247..6920
                     /gene="asnB"
                     /locus_tag="PKH68_04450"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HNX12222.1"
                     /translation="MCGIVCTFNIKQPIEVLRPQVLQMSKTIRHRGPDWSGIFANDNA
                     ILAHERLAIVDPRSGRQPLYSKDGKLVLAVNGEIYNHREIRKQFEGKYEFLTQSDCEV
                     ILALYREKGPAFLEDLNGIFAFALYDIENNRFLIGRDHIGIIPLYQGRDKDGAFYVAS
                     ELKALEGYCEKIEEFLPGQYYYSPDGKPTQWYSRDWMEYDNVKDNTSSVDELRQALED
                     AVERQLMSDVPYGVLLSGGLDSSIISAVAKKFAARRIESGNTEDAWWPQLHSFAIGLE
                     GSPDLAAARKVAEHIGSVHHEINFTVQEGLDAIRDVIYHIETYDVTTVRASTPMYLLS
                     RYIKSMGVKMVLSGEGADEIFGGYLYFHKAPNAEEFHKETVRKLSKLHLYDCLRANKS
                     LASWGVEGRVPFLDKEFMDVAMRLNPTDKMAGNGKIEKHILRQAFENYLPSEIAWRQK
                     EQFSDGVGYNWIDTLKEIASEKVTDEQMENVATRFPIQPPMTKEEYHYRSIYSELFPS
                     DSAALCVPSVKSVACSTPIALEWDEAFKKMADPSGRSVSSVHEQGYKQK"
     gene            complement(7018..8217)
                     /locus_tag="PKH68_04455"
     CDS             complement(7018..8217)
                     /locus_tag="PKH68_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNX12223.1"
                     /translation="MRKIKNYIIVILLTISGLSAVNAQGITLSDSATLSLITCSPGPQ
                     IYALFGHTAIRLVDPTRGLDIVFNYGMFNFNKPNFYLKFIQGATDYELGAYETKYFLP
                     EYRERNSSVTEQMLNLTTVEKQQLADALFVNYQPENREYRYNFVYDNCSTRPRDKILS
                     VIKDKVVYDYVSEPQTFRNWVGTYTGENTWAKFGIDMLLGREADELSTRWSSMFLPEV
                     LCREFGAVKIIAHDGTTRSLVNAEKIIVPRQDSIVKTNFLQLPITVTLTVLLLGVLLI
                     FYEKRRKKYYKIIDSVLLIVSGLAGVIIFYLMFFSVHPLVHSNFNLLWCNPLNVLVGL
                     FLWNRKLRSVINYFQLANVLLFFGALLVFVLSVQALNVAFIPLIVLLLVRSVNWIMKK
                     SKQLKKL"
     gene            8471..9172
                     /locus_tag="PKH68_04460"
     CDS             8471..9172
                     /locus_tag="PKH68_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX12224.1"
                     /translation="MTKKQISLLRPIEDVLSFWDVLTPDERNFILNTYTLHKYKKNEV
                     IQCEGDEPTHLMILAEGKVKVYKNGFGNRAQIVRMLKPGEHFGYRAIIANEVNSTTVT
                     AFEDSTIYLISGDIFLSILRHNNAFCYRFLEELSFDLAESDARTVNLTQKHIRGRLAE
                     SLLFLKDNYGLEEDGATISIYLSREDLANHSNMTTANAIRTLANFVNESLIAMDGRKI
                     KLIDEEKLRRISKLG"
     gene            9243..11450
                     /locus_tag="PKH68_04465"
     CDS             9243..11450
                     /locus_tag="PKH68_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX12225.1"
                     /translation="MTPTEIHSTYKQALAYLSQGNLKNAFDKTTELSNELQSPVYRDS
                     LQNMQQSYRYMLQYFLKGINDPERKTVYNKLIARLFVLISSLREDLLTRDSSNFEFSQ
                     KRYFPFTPQPAYSELKKGLMRMEDLVELGDAETIDTQRENQIQLRFESAIHYLFNYFW
                     LKSNYDSLEIMELYRYVMDDASTDSVAKSMLVSALTLNLWRHFDENKILMLLDACTST
                     DVYARQRALTGLCFVLAKYNRFLPYFPAIRNRLVLLADDAVITEQLQNIIIQIIATSE
                     TEQVSKKLQEEIFPELMKLRPLMDEGLNPENLMNLDEWGEINPQWEEMIEKSGASDKI
                     QEFAEMQMEGADVYMSTFAMLKSFPFFSDFSNWFMPFDTENLAVRPLFATEQKSLITA
                     FLSSNVMCNSDRYSFCLSVLQMPELQRSMMKQSFGEAAEQMEEISKDEALHSPNVQAK
                     NISKHYVQDLFRFFKLNPHHGDFTDMFSTSLIMHKTYLFDLLSIDDTLKSNIAEYYFT
                     KKLYLQALELFEEINKEGEISAALYQKMGYAYQQTSRLMEALDAYKKADLIQPDDFWT
                     NRKIALCYRLMGNAEKALEIYRHTNFIKPDQLSIKLQIVSCLKELRKTEEALRELMKL
                     HEEYPDNHKIIREIMHCSFIAKNPAQAKYFATLLLETKNLTADDYCKAGHLEWCLNKQ
                     QKAVGHYKQCLQLLSNNWTQFNTLFLQDKNTLISNGIPESDIPLMLDGLKYEE"
     gene            complement(11484..12713)
                     /locus_tag="PKH68_04470"
     CDS             complement(11484..12713)
                     /locus_tag="PKH68_04470"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNX12226.1"
                     /translation="MNLKKTLRIFFLSLFLAHTAAAQNSENYTFKGEFIYGTIIKHTD
                     HLKNIVKGPVMGGEVAVEWQSADGWHRYFNYPYLGVGAAYLNLGNPDTLGNALAIYPY
                     INFPIVRTGRFSVNFKAGAGLSYVTKTFADFRENVMRVSSDPLFLDYKKSNAAIGSPL
                     NVYFAAGFNADVPLSDNLSFTAEYGWNHISNGMIKAPNSGINMLNAFIGLKYTPNFDA
                     RRNEYLRYFYAPDLPRDITCEITVSGGLRQRYMRDGGEGYGKTFPIASLAAGVYKPLT
                     NFYRMGIGGDFFYDGVYTTGLSSGYKREYITVNELKNKLRFGISWQNELIMGRFTAGI
                     HAGVYLFNPVKNLEPFESPSNKGIIYSYDPEKEVGWFYTRVSGKYLLTKHIYASIGLK
                     THLQKAEFIEWGLGYRF"
     gene            12792..13517
                     /gene="gldF"
                     /locus_tag="PKH68_04475"
     CDS             12792..13517
                     /gene="gldF"
                     /locus_tag="PKH68_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNX12227.1"
                     /translation="MLSVLKKELRSFFSSATGYIVIGIFLLLTGLFLWVIPGEFNILE
                     AGYANTDGLFYLAPWLFLMLCPAITMRFFAEEKQTGTWELIITKPLTTRQIVLGKYFA
                     GTILVVLALIPTLIYFYTVSYLAEPIGNVDAGQFWGSFIGLIFLAAVYTAIGTFTSSL
                     TKNQIIAFIFALVLCFFFYYGFDLLTGFVSGGTKMQFIENLGIHAHYKSISRGVIDSS
                     DVIYFLVLSTVFLELTVLKLKRS"
     assembly_gap    13691..13790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13878..14633
                     /gene="surE"
                     /locus_tag="PKH68_04480"
     CDS             13878..14633
                     /gene="surE"
                     /locus_tag="PKH68_04480"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNX12228.1"
                     /translation="MKPTILITNDDGVNAPGIRVLIRLMKQIGNVIVVAPDGPRSGQS
                     NAITVHTPVRYRLLESSDGLTIYSCNGTPTDCVKLAFNDIIDQNPDLVVAGINHGSNS
                     AINVIYSGTMGAVLEGCQHGVASIGFSLCDHAHEADFTTFEPYILQIARETLKNGLPE
                     NICLNVNAPVGEILGTRITRQCNGVWVKEFARRTDPVGHDYYWLTGNFENHEPDAEDT
                     DEWALANGYVSVVPTTTDMTAHRMIERIKSWKL"
     gene            14696..14980
                     /locus_tag="PKH68_04485"
     CDS             14696..14980
                     /locus_tag="PKH68_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12229.1"
                     /translation="MKKFDHFLYGFIPGLLMPIGFLWIYLNRFYPADLTFIEILKQLY
                     PSAMLGKLLLLSIMLNLVGVFIFYKQDSLRLGIGMMMGAMPYLIGALMMM"
     gene            15050..>15267
                     /locus_tag="PKH68_04490"
     CDS             15050..>15267
                     /locus_tag="PKH68_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12230.1"
                     /translation="MNKNQDIITNQQVSTNPKVPPSGGFRGASSLPSGGGGPSYFLIA
                     GEASGDLHASNLMRELKKIDAEAEFCFLG"
BASE COUNT         4453 a         3308 c         3384 g         4022 t
ORIGIN      
        1 taccacagca tgctgatgga accctgggac ggacccgcta cactgctttt cagcgacgga
       61 cggtttgcag gcggtatgct cgaccgcaac ggacttcgac ccgctcgtta caccatcacc
      121 aacgatgacc gcatgattat tgcctccgaa acgggaacta ttgaactgga agcatcgaat
      181 atcaaagcac ggggcaggtt aagacctggt aaaatgctga ttgtggacac caaaaccggt
      241 cagatttttt acgataatga gttgaaagac ggattggcaa aagcttttcc gtaccgcgat
      301 tggttgaata aaaattgcag caatctggac gttatttcgt ccggaagaag cgttaacaac
      361 gatatttcaa attacgaaac attgatgact gctttcggtt attcggtgga agacattgaa
      421 aaactgatac ttccgatggc tgatgaagga aaggaaccga cttcatccat gggaaatgat
      481 gtgacactgg cagcattctc caacaaacct cagcgtttgt tcaactattt ccggcagcaa
      541 tttgcgcagg ttaccaatcc tccgattgat cctatacgtg aagaactggt gatgtcactc
      601 accggttatg tgggttctgt tcatcaaaat ttattggagc aaattcccga attatcaaaa
      661 attgtaaagc tgaaaagtcc gattttgacc aatacacagt tcgatatctt actgaatttg
      721 aggtacaaag gtttctctac cgctgttttg cccatgcttt tcgacccgaa aggcggcact
      781 cgtggacttg aaaaagccat cgacgaactt tgtattacgg tggaaaaagc cgtggatgaa
      841 ggcaaaaact acattgtgct cagcgaccgc ggtgtggatg ctgctcacgc tcctattcca
      901 tcgttgctgg ctgtttcagc cgtacattta tacctggtgg aaaaacgcaa gcgaatgcag
      961 attgatattg tggtggaaag tgccgagcca cgcgaagtga tgcattttgc cctgctgttc
     1021 ggctttggtg ccaatgctgt taatccttat ctcgcatttg ctgtgctcaa caggaaagta
     1081 aaatccgaag atattcagct tgatttcgat acagccaaga aaaattacat caaagccatt
     1141 aacaagggat tgctgaaagt gctttcgaaa atgggaaatt ccacgctgag aagctatcgt
     1201 ggcgctcata tttttgaggc catcggtatt tcgtcacggg tactgaacgc ctatttcaag
     1261 gatatttctt ccaaaattga aggaattgat atggaggaca ttacccgcga agtgctgcaa
     1321 cctttctacg aagcatttgc tgctgataac aacgaaccat ttcacctgaa aaatctgggt
     1381 cactacgcat atcgcaatga cggcgaatac catgcctgga accccgaaac gattgcccga
     1441 ctgcaaattg ccactaaaac aggcgattac agtaaataca aagaatattc agcgttggtg
     1501 gacaataaat ccaatccggc gtttatccgt gatttgctgg attacaaacg caatccgatt
     1561 gatatttcgg aagtggaacc ggcagaaaac atcatgaaac gtttctgtac gggtgccatg
     1621 tcctatggtt caattagtcg cgaagcacat gaagccatgg caatagccat gaacaaaatc
     1681 ggtggacgaa gcaatacggg tgaaggcggt gaagatcccg aacgatataa aaaacgggat
     1741 gacggactga gtacacgttc ggctattaaa caggttgcat ccggacgttt tggcgtaacg
     1801 gcagagtatc ttgtaaatgc cgacgaactt caaattaaaa ttgctcaggg tgcaaaaccc
     1861 ggtgaaggcg gacaacttcc gggttacaaa gtggataaaa tcattgccaa aacgcggcat
     1921 tccattcccg gtatttcact tatttcgcct cctccccatc acgatattta ttccattgag
     1981 gatttggcac agttgatttt cgatttgaaa aacatcaatc cgaaagccat tgtaagcgtc
     2041 aaactggtat cggaaagcgg tgtgggaacc atagcggccg gtgtagccaa agccaaagca
     2101 gatttaattc tgatcagcgg agccgaaggc ggcaccggtg caagcccttc cagttctatc
     2161 aaacatgccg gactgccagt ggaaattgga ctggccgaaa cgcaacaaac actggtaatg
     2221 aataacctgc gcgggaaaat ccgcctgcaa accgacggac agctgaaaac cggccacgat
     2281 atcatcatat ctaccctgtt gggagccgaa gaatttggtt ttgccaccag cgcactcatc
     2341 gttttaggtt gcgtgatgat gcgcaagtgc cacctgaaca cctgtcccgt gggagttgcc
     2401 actcaaaacg aagaacttcg caaacgcttt gtaggcagat acgaatacct ggtgaatttc
     2461 tttacgttcc tggccgaaga aacccgcgag cacctggctc agcttggttt caagaccctg
     2521 gacgaagctg tgggacgggc tgatttactc gaacgcaaac agttgcccga ttatccgaaa
     2581 accgaaaaaa tagatttatc cagaattaca tttatgccca aagaagctgc tgtaaatgca
     2641 cttcgtaaga catccgacca ggatcacaaa ctcgatagca ttctcgaccg ggaacttatc
     2701 agcaagtcgc ttccagcgct tgacctctgc atgccggtta ccattcagtc gaaaataaaa
     2761 aataccgaca gaactaccgg tgccatgctt tcgggtgaaa ttgcaaaacg ttacgggcaa
     2821 gccggactgc cggaaaatac gatttccatt cagtttgaag ggtcggccgg acaaagtttt
     2881 ggagcttttc tgtccaaagg aatgactttc cacttgcagg gcgatgccaa cgattatctc
     2941 ggcaaaggac tttcgggagg acgaattatc gttacaccgc cgaaagggag cacctttgca
     3001 cccgaagaaa atattttgtg tggaaatacg ttactttatg gcgcaacttc cggagaagtg
     3061 tttatcaatg gagtggttgg cgaacgcttc tgcgtgcgca attcaggcgc aacagccgtg
     3121 gtggaaggag ccggcgatca ttgctgcgaa tacatgaccg gtggccgaac cgtagtactc
     3181 ggcataaccg ggcgaaattt tgcagccggt atgagtggcg gtgtggcgta tgttctcgat
     3241 gaaaaaggcg attttgacta tttctgcaac atggaaatgg tggaactttc gctcatcgaa
     3301 gaaagcggtg acaataaaga attgcacggt ttgattgaag cacattacaa atataccgac
     3361 agtccgctgg caaagaaaat actggacagc tggaacgaat atgtggataa attcatcaaa
     3421 gtagttccta ttgaatacaa gaaagtattg gaagaagaaa aaatagaagc aataaaacga
     3481 aaaattgcaa gtgtagagag agactattaa ccggcccctc taaatctccc caaaggggag
     3541 actttaagac gagctgacta tgtatgaaga agaaaaaata aaaacaaaac taactacaag
     3601 ttcccccttt gggggaattt aagggggctt acatatggga aatccaaaag catttatatc
     3661 tattccgcgg aaggaagcgg gatatcgccc ggttctcgaa cgaatttatg atttcggaga
     3721 agtggagcaa acactcaatc aggaagaccg caaattgcag gcatcgcgct gcatggattg
     3781 tggcattcca ttctgccact gggcatgtcc gctcggcaac aaaatgccgg aatggcagga
     3841 tctgatttat aaaggcaggt ggaaagaagc catcgaagtg cttcatgaaa ccaataattt
     3901 cccggatttt accgggcgca tttgtccggc accgtgtgaa aaatcgtgcg ttcttaatct
     3961 gagcgaggca ccggtaacca ttcgtgaaaa cgaagcagct gttaccgaag tggcattcat
     4021 cgaagggatg atcaaggcca gaccgcccaa aacccgaacg ggcaaaaaag tggctgtgat
     4081 tggttccggt ccggcaggat tggctgctgc tcagcaactc aaccgaaaag gacacaccgt
     4141 aaccgttttt gaaaaagaca acagcatcgg cggattgctg cgctacggca ttcccaattt
     4201 caaactgaat aaaaacatta tcgaccgccg gttggctcag ctcgtagaag aaggaattat
     4261 tttcaaaccc aataccgaaa tcgggaaaac catttcggga actgaaataa tcaagaaatt
     4321 tgatgctgtt tgtctggccg tcggtgcggg tcatccgcgc gacatttctc cggctggcag
     4381 agagttgaag ggcatttatt ttgccatgga ttttctcgca cagcaaaatc aggtgattat
     4441 ggaggaaaag cttacaaaac ccgaacgaat tactgccaaa gacaaacacg tgcttgtgat
     4501 tggaggcggc gataccggtt ccgactgcgt gggtacctca ttgcgtcagg gtgcggttaa
     4561 agttacgcag attgaaattc tgccgaaacc tccggcaggt aaaaatcccg aaactccctg
     4621 gccttattat cccaacattc tgaaaacatc cagttcacat ctcgaaggtt gcgagcgaaa
     4681 atggtcactg aatacggtgg aattcaaagg taataacgga cagttggagg aagtggtggt
     4741 agaaaaagtg gaatggaaaa aagacgaaag tggtcggttt acaatgattt caaccggtaa
     4801 aaccgaaata atcaaggctg atttagtgtt tttagccttg ggatttgttc atccggtgca
     4861 cgaaggatta ctgaccgaac tgggtgtaaa tttagatgca cgtggaaatg tggctgttga
     4921 ccgccaaaat caaagcagcg tggcaaaagt atttgctgcg ggcgatgcag ccgtgggtgc
     4981 cagtctggta gttcgtgcca ttgcttcggg tcgcaacgtg gccgaagata ttcataagaa
     5041 actgggcggc ccccctaccc cctaaagggg gagttctttg agtgcaaaaa ttttcatctg
     5101 aaaattaagc acaactattt taccgaaggt ttataatcgt tcaatatcaa ttgattataa
     5161 taaatctcaa aattcaacgc aaaattaatc agacttaaaa cacgcccaaa actccccctt
     5221 cagggggctg gggggctttt ttcattatgt gtggaatagt atgtacattt aatataaaac
     5281 aacccattga ggtattgcgt ccgcaggtac ttcaaatgtc gaaaaccatc cgtcaccgcg
     5341 gtccggactg gtcgggaatt ttcgcgaatg acaacgccat tctggctcac gagcgtctgg
     5401 caatcgttga tccgcgttca ggcagacaac cgctttacag caaagacggg aaactggtac
     5461 tggcagtgaa tggagaaatt tacaaccatc gtgaaattcg aaaacaattc gagggaaagt
     5521 atgaatttct cacacaatca gattgtgaag tcattctggc actttatcgg gagaaaggtc
     5581 ccgcttttct ggaagacctg aacggaattt ttgcttttgc cctgtatgat attgaaaaca
     5641 accggtttct gataggtcgt gaccatatcg gtattattcc gctatatcag ggccgggaca
     5701 aagacggtgc tttttatgtg gcatccgaac taaaagcgct cgaaggttat tgcgagaaaa
     5761 ttgaagaatt tctgccgggt caatattatt acagtcctga tggcaaacca acgcagtggt
     5821 attcccgcga ctggatggaa tatgataacg tgaaagataa tacttcgagt gtggatgaac
     5881 ttcgtcaggc actggaagat gcagtggaac gtcagttgat gtctgatgta ccttatggtg
     5941 tactgctttc gggtggtctc gacagttcca ttatttcggc tgtggccaaa aaatttgccg
     6001 ccaggcgtat tgaaagcggt aacacagaag atgcgtggtg gcctcagctt cattcctttg
     6061 ccatcggcct ggaaggttca cccgatctgg cagcagcccg caaagtggca gaacatatcg
     6121 gttcggtgca tcatgaaatt aatttcaccg tacaggaagg actggacgcc atccgcgatg
     6181 taatttatca tattgaaacg tatgatgtta ccaccgtccg ggccagtaca ccgatgtatc
     6241 tcctctcccg ttacatcaaa tccatgggtg tgaaaatggt gctttcgggc gaaggagccg
     6301 acgaaatatt cggcggatac ctgtatttcc acaaagcgcc caatgcggag gaatttcaca
     6361 aggaaaccgt tcgcaagctt agcaaacttc atctttacga ttgcctgcgt gccaacaagt
     6421 cactggcatc gtggggtgtg gaaggacgtg taccgtttct cgacaaagaa tttatggatg
     6481 tggccatgcg gttaaacccg actgacaaaa tggcaggtaa tggtaaaatt gagaaacata
     6541 ttctgaggca ggcctttgag aattacttac cgtcagaaat cgcctggcgt cagaaagagc
     6601 agttctccga cggtgttggt tacaactgga ttgacacatt gaaagagatt gcttcagaaa
     6661 aagtaaccga cgagcaaatg gagaatgtgg caacgcgctt tccgatacag ccacccatga
     6721 ccaaggaaga atatcattac cgcagcattt acagcgaact tttcccatct gatagtgccg
     6781 cactctgcgt accttcggtt aagtcggtag cttgcagtac accgatagca cttgaatggg
     6841 atgaagcatt caaaaagatg gccgatcctt caggacgctc cgtgagttcg gtacacgaac
     6901 aggggtataa acagaaatag ttttatcgaa gaacaaaaaa aatcgggtta caagatgttg
     6961 taacccgatt tttaatatta cttattagct ttatttcaca actaatttca atcgaaatta
     7021 taactttttc aattgtttac tttttttcat gatccagtta accgaccgca ccagcagcaa
     7081 cacaatcaag ggtatgaatg ctacattaag tgcctgaaca gacagtacaa ataccaacag
     7141 ggcaccaaag aaaagcagca cattggccaa ctgaaaataa ttgatcactg aacgcagttt
     7201 tctattccac agaaacagtc ccaccaacac attcaacgga ttgcaccaca gcagattaaa
     7261 attggaatga acaagcggat gaactgagaa aaacatcagg taaaaaatga taacaccggc
     7321 cagtccgcta acgataagca gcacactatc aattattttg tagtattttt ttcttctttt
     7381 ctcataaaaa atcaggagca ctccgagaag caaaacagtc agcgtaacgg taattggtag
     7441 ttgtaaaaaa ttggttttaa cgatgctatc ctgtcgggga acaataattt tttcagcatt
     7501 taccagtgat cgtgtggtac catcgtgggc aataattttc actgcaccaa actcgcggca
     7561 caatacttcg ggtaaaaaca tggatgacca gcgggtagac agttcatcgg cttcgcggcc
     7621 gagcaacatg tcgatgccaa atttagccca ggtattttca ccggtgtaag ttcccaccca
     7681 gttgcgaaat gtctgaggtt cactcacata gtcgtacacc actttatcct taattaccga
     7741 cagaatttta tcacgtggtc gggtggagca attgtcgtac acaaaattgt agcgatattc
     7801 gcggttttcc ggttgatagt tgacaaataa tgcatcggca agttgttgtt tctcgactgt
     7861 ggtgagattg agcatctgct ccgtaaccga tgaatttctt tccctgtatt cgggcaaaaa
     7921 atatttagtt tcatacgccc ccagttcata atcagtagca ccctgaataa atttcagata
     7981 gaaattcggt ttgttgaaat tgaacatacc gtagttgaat acaatgtcca atcccctggt
     8041 agggtcaaca agccgaatgg cggtatgtcc gaaaagggcg taaatctgag gccccggcga
     8101 gcaggttatc aggctgaggg tagccgagtc gctgagcgta atgccctgtg catttacagc
     8161 cgacaaacca ctaattgtca gcaaaataac tatgatataa ttttttattt ttctcacaat
     8221 aattcatttt tttaattcat gtaaaagtaa tttatttttt ctattagttg agcggtcaat
     8281 tctaaaaaaa ataaaacaaa aaccggaata gttcaattat taatatacct aaaatcggta
     8341 agatacaaag ttaaatattg gctaaatatt cacaaataat cagattttcc cccttaaaga
     8401 aatatttttt gaaatttttc cttaaatttg tgctaaagaa cattgttttc taacatatct
     8461 cttaattgcg atgacaaaaa aacaaatttc attacttcgt ccgatagaag acgttttatc
     8521 attctgggat gtacttactc ccgacgaacg gaactttatt ctgaacacct atacccttca
     8581 taaatacaaa aaaaatgagg tgatacagtg cgaaggtgat gagccaactc acctgatgat
     8641 attagccgaa ggaaaagtga aggtgtataa aaatggtttt ggcaaccgcg cacaaatagt
     8701 gcgtatgctc aaacccggtg aacatttcgg gtatagagct atcatagcca acgaagtgaa
     8761 cagcacaacg gtaacggctt ttgaagattc tacgatttat ttgattagtg gagatatttt
     8821 cctgtcaatt ctccgtcata ataatgcctt ttgttaccgc tttctggaag aattgtcatt
     8881 tgatttggca gagtccgatg cccgaactgt caatttgaca caaaaacaca ttcgcggacg
     8941 tttggcagag tctttgttat ttcttaaaga caactatggt ctggaagagg acggggcaac
     9001 catcagcatt tatctttcgc gtgaagatct ggcaaatcat tccaatatga ccactgccaa
     9061 tgctatacgc acgctggcaa actttgtcaa cgaatcgctc atagctatgg acggacgaaa
     9121 aatcaaatta attgacgaag aaaaactccg gagaataagt aaattgggtt aatgtttttt
     9181 attaaatccc ggtggcttaa gcatcagtag tatttctttt tcgcaatcgt gtaaaaaaaa
     9241 acatgacacc tactgaaatt cacagcactt acaaacaagc gctggcttac ctgtcgcagg
     9301 ggaatctgaa aaatgcgttc gacaaaacaa ccgaactgag caacgagcta cagtcgcctg
     9361 tgtacaggga cagcctccag aatatgcaac aaagctaccg gtatatgttg cagtactttc
     9421 tgaagggtat aaatgacccg gaacgaaaaa ctgtgtacaa taaactcatt gcccgattgt
     9481 ttgtactgat ttccagttta cgtgaagatt tgctcacccg cgactcctcc aattttgaat
     9541 tttcccaaaa gagatatttc ccttttactc cccagccagc ctactccgaa cttaaaaaag
     9601 ggctgatgag aatggaagac ctggtcgaac tcggcgatgc cgaaacgatt gacacccagc
     9661 gcgaaaatca gatacagctt cgttttgaat ctgccatcca ctatttgttc aattatttct
     9721 ggctgaaatc caactacgat tcactcgaaa taatggagct ttaccgctac gtgatggacg
     9781 atgccagcac cgattcggta gccaaatcga tgcttgtttc cgctctgacc ttgaatctgt
     9841 ggcgccattt tgatgaaaac aagatactga tgctgctgga tgcctgcact tcgaccgatg
     9901 tgtatgcacg ccaacgggcg ctcaccggac tttgtttcgt actggctaag tacaaccgct
     9961 ttctgcccta ttttccggct atccgcaacc ggctggtatt gctggctgac gatgccgtta
    10021 tcaccgaaca acttcagaat attatcattc aaataatagc cacctccgaa accgagcagg
    10081 tttcaaagaa acttcaggaa gagatttttc cggaactgat gaagcttcga ccacttatgg
    10141 acgaaggtct aaatcccgaa aacctgatga acctggatga atggggcgaa ataaaccccc
    10201 agtgggagga aatgatagaa aaatcgggtg cgtcggataa aattcaggaa tttgccgaaa
    10261 tgcagatgga aggcgccgat gtatatatga gtacctttgc catgctcaaa tcatttccgt
    10321 ttttttccga tttttccaac tggtttatgc cttttgatac cgaaaacctg gcggtacgac
    10381 cacttttcgc tacggagcaa aaatcactga taacggcttt tctgagttca aatgtgatgt
    10441 gcaattccga ccgttattcc ttctgtctga gcgtattgca aatgccggaa ctccagcgca
    10501 gcatgatgaa acagtcattc ggcgaagccg ccgagcaaat ggaagagatc agcaaggacg
    10561 aagcccttca ctcgccgaat gttcaggcaa aaaacatatc gaaacattat gtgcaggatt
    10621 tattccggtt tttcaaactc aatccgcatc acggggactt taccgatatg ttttccacct
    10681 cactcatcat gcacaaaacg tatttgtttg atttgctgtc cattgacgac accctgaaat
    10741 ccaacatcgc agaatactat ttcacaaaaa aactttatct gcaggcactc gaattgtttg
    10801 aagaaattaa taaagaaggg gaaatttcgg cagcattgta tcagaaaatg ggttatgcct
    10861 atcagcagac ctcccgactt atggaggcat tagatgccta caaaaaggca gaccttatac
    10921 aacccgacga tttttggacc aaccggaaaa ttgcactttg ttaccggctg atgggtaatg
    10981 cggaaaaagc gctggaaatt taccggcata ccaactttat aaaacccgac cagctttcga
    11041 taaaacttca gattgttagt tgcctgaaag aactccgaaa aacggaggaa gccctgcgtg
    11101 aactgatgaa attacatgag gaatacccgg acaatcacaa gattatccgc gaaataatgc
    11161 attgttcgtt tattgccaaa aatccggctc aggctaaata ttttgccacc ctgctgctgg
    11221 aaaccaaaaa tctgactgcc gacgattact gcaaagccgg tcatctagaa tggtgtctga
    11281 acaagcaaca aaaggcagta ggtcactaca aacaatgcct gcaactgctc agcaacaact
    11341 ggacgcaatt taacacctta tttcttcagg ataaaaacac actgatatcc aacggaatac
    11401 ccgaaagcga catcccgctg atgcttgatg ggttgaaata tgaagagtaa aacaaaagcc
    11461 gctgaaaaac ggcttttgta cttttaaaac ctgtacccca gcccccactc tataaattcg
    11521 gccttttgaa ggtgggtttt aagcccgata gatgcgtaaa tgtgtttggt tagcaggtat
    11581 tttcccgaaa cgcgggtgta aaaccaaccc acctcttttt caggatcata ggagtagata
    11641 atgcctttgt tggaaggaga ttcaaacggt tcaagatttt taacaggatt gaacaggtaa
    11701 acaccggcat gaattccagc ggtaaatcgt cccataatca gttcattctg ccatgaaatg
    11761 ccaaatcgaa gtttattttt caactcattt actgttatgt attcccgttt ataacctgaa
    11821 cttaatccgg tagtataaac accatcatag aagaaatcac caccaatacc cattcggtaa
    11881 aaatttgtaa gtggcttata cacccctgca gcaagcgatg ctattggaaa tgttttacca
    11941 tatccttcac ctccgtctcg catatatcgt tgccgcaatc cacccgaaac agtaatttca
    12001 caggtaatat cgcgtggcaa atccggagca taaaaatacc ttaggtattc atttcgacgg
    12061 gcatcgaagt ttggtgtata tttcaggccg ataaatgcat tgagcatatt gatacctgag
    12121 ttgggcgctt tgatcattcc gttggaaata tgattccaac cgtattcagc cgtaaaactc
    12181 aggttatcag acagggggac atccgcatta aaacctgctg caaaatatac attcagcggt
    12241 gatccgatag ctgcattact ttttttataa tcgagaaaaa gaggatcact tgaaactctc
    12301 atcacgtttt ctctgaaatc agcaaatgtt ttggtgacat aacttagtcc ggctccggct
    12361 ttgaaattga ccgaaaaccg tccggtgcgg acgatgggga aattgatgta tgggtaaatt
    12421 gccagggcat ttccgagtgt atcggggtta cccaaattga gatatgcagc acccacaccc
    12481 aggtatggat agttaaaata ccggtgccat ccgtcggccg actgccattc cacggctacc
    12541 tcaccaccca tcacaggtcc ttttactatg tttttaaggt ggtcggtgtg tttaattatt
    12601 gtgccgtaaa taaattcccc tttgaaagtg taattctcgg aattctgagc agcagcggta
    12661 tgtgccagaa aaagtgacag aaagaatatt cgtagcgttt ttttcaggtt catgcagggt
    12721 gtataataca gcttttagaa taaaatttgc tatctttgac atcgcaaatt taagaaaaat
    12781 tgtctcagta aatgttatct gtcttaaaaa aagaactccg cagctttttc agcagtgcca
    12841 ccggatatat tgttatcgga atatttttgt tgcttaccgg gctgtttttg tgggtcattc
    12901 ccggtgaatt caacattctg gaagccggat atgccaatac cgacggactg ttttacctgg
    12961 ctccgtggct gtttctgatg ctttgtccgg ctatcaccat gcggtttttt gccgaagaaa
    13021 agcaaaccgg aacctgggaa ctgatcatca ccaaaccact gacaacccga caaattgtgt
    13081 tgggaaaata ttttgccgga acaatactgg tcgtactggc attaatcccg acattaatct
    13141 atttctatac cgtgagctac ctggccgagc ctatcgggaa tgtggatgcc ggtcagtttt
    13201 ggggatcgtt tatcggactg atttttcttg cagccgttta tacagctatc ggcaccttta
    13261 catcgtcact gacaaaaaat caaatcattg cttttatttt tgctttggta ctttgtttct
    13321 ttttttacta tggatttgat ctgctcaccg gctttgtgag tggtggaacg aaaatgcagt
    13381 tcatcgaaaa tctcggcatt cacgcacact ataaatccat cagccgcgga gtaatcgact
    13441 cgagtgacgt gatctatttt ttggtgctca gcactgtatt tttagaatta actgtattaa
    13501 aactgaaacg ttcttaataa gtttgacgtt taatgtttaa tttctaacgt ttaatgttta
    13561 atgtctaacg tttaatgttt aatgttcagt gttcaattgt ttacaaattt ctgatttcta
    13621 atttctaatt tctgacttct aatttctaat ttctgacttc taatttctaa tttctgactt
    13681 ctaatttcta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cttctaattt
    13801 ctaatttctg acttctaatt tctaatttct gacttctaat ttctaatttc tgacttctaa
    13861 tttctatttg ctaacgtatg aaacctacta tattaataac aaacgatgac ggcgtcaatg
    13921 ctccgggcat tcgggtactg atccggctga tgaagcaaat cgggaatgta attgttgtgg
    13981 cacccgacgg accgcgttcc ggacaatcga atgccatcac ggtacatacg cctgtgcgat
    14041 accgactgct tgaaagcagc gacggactga ccatctattc gtgcaacggc actcctaccg
    14101 actgcgtaaa actggctttc aacgatatca ttgaccagaa ccccgatctg gttgttgcgg
    14161 gcataaatca tggctctaat tcggctatca atgtgattta ctcgggtacc atgggtgcgg
    14221 tactggaagg atgccagcac ggagttgcat ccatcggatt ttcgctgtgt gatcacgccc
    14281 acgaagccga tttcaccaca ttcgagccct atatcctcca aatagcccgc gaaaccctga
    14341 aaaacggctt gcccgaaaat atttgcctta acgtgaatgc tccggttggc gaaatactgg
    14401 gtacccgcat cacacgccag tgcaacgggg tgtgggtcaa ggaatttgcc cgaagaaccg
    14461 atcccgttgg acacgattat tactggctga ccggtaactt cgagaatcac gaacccgatg
    14521 ctgaggatac cgatgaatgg gcgctggcta acggctatgt ttcggtggtt cccacaacta
    14581 cggatatgac tgctcacagg atgattgaga gaattaaaag ctggaagtta taatctgacg
    14641 ttttagctta ctgctatttc gcatttcaat tcattaaatt ccagttctgt acataatgaa
    14701 aaagtttgat cattttctct acggatttat acccgggtta ttgatgccaa tcggtttttt
    14761 atggatttac ctgaaccggt tttaccctgc cgatctgaca ttcattgaga tactgaagca
    14821 gctctaccca agtgccatgc ttggaaaact actattgctt tccatcatgc tgaatttggt
    14881 gggcgtattt attttttaca aacaggacag tctcaggttg ggaataggaa tgatgatggg
    14941 agcaatgcct tatttgattg gggcgttgat gatgatgtaa tggccccctg ccccccaaag
    15001 ggataagccc cctaaatccc ccaaaggggg acttaaagaa aaaagagtaa tgaataaaaa
    15061 ccaagatatt atcacaaatc aacaagtatc aacaaatcca aaagtccccc cctcgggggg
    15121 atttaggggg gcttcttcct taccttcggg gggtgggggg ccctcctatt tcctcattgc
    15181 cggtgaagct tcgggcgatt tgcatgcttc caatctgatg cgtgaactga aaaagattga
    15241 cgctgaggct gagttctgtt ttctcgg
//
LOCUS       DAOPZK010000048        15185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_4239_length_15185_cov_23.442631, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000048 DAOPZK010000000
VERSION     DAOPZK010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15185
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_15185_cov_23.442631"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1007
                     /locus_tag="PKH68_04495"
     CDS             87..1007
                     /locus_tag="PKH68_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX12231.1"
                     /translation="MKILIATDKPFAKVAVDGIRNEIEGVGYELALLEKYTEKQQLLD
                     AIADADAVIIRSDIVDEEVIRAGKKLKIVVRAGAGYDNVDLEAATKAGVCVMNTPGQN
                     ANAVAELVFGLLVYAVRNFYNGTSGTELMGKKLGIHAYGNVGRNVARIAKGFGMEVLA
                     YDAYCRAEDIKAEGVNPTCSVKTLYQGSDIVSVHIPLTAETRQIINKELLELLPKNAV
                     MVNTARKEVINEADLLQHMIDNPKFKYVTDVASDNEAEMKEKAGEQYFSTAKKMGAQT
                     AEANVNAGIAAAQQIVDFLKNGNEKYRVNK"
     gene            complement(1216..2577)
                     /locus_tag="PKH68_04500"
     CDS             complement(1216..2577)
                     /locus_tag="PKH68_04500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNX12232.1"
                     /translation="MKIAIIGTGYVGLVSGACFAEMGVDVTCVDVNKQKIDNLLKGII
                     PIYEPGLEDMVQRNVKAERLHFTTRLEDMLDEVQVIFSAVGTPPNEDGSADLKYVLQV
                     AAEVGRKMNNYVLLVTKSTVPVGTANLVKQTIQAELDLRGVSIPFDVASNPEFLKEGD
                     AINDFMSPDRVVVGTESEKAKELLTKLYRPFLLNNFRVIFMDIPSAEMTKYAANSMLA
                     TRISFMNDIANLCEIVGADINMVRKGIGSDVRIGGKFLYAGSGYGGSCFPKDVQALIR
                     TAQENGYELRLLRAVEDVNNIQKEVLFRKLTKRWGMEMNGKKVAIWGLAFKPNTDDIR
                     EAPAFVVIEKLLRAGATVRVFDPIAMPASKERIEFLEKEQKNFPEAEKLDFSKVYFAN
                     DIYDAADEADALLLMTEWKEFRMPSWNVIKKSMKNPLVLDGRNIYDKTEMKEMGFEYE
                     GIG"
     gene            complement(2914..3543)
                     /locus_tag="PKH68_04505"
     CDS             complement(2914..3543)
                     /locus_tag="PKH68_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNX12233.1"
                     /translation="MEQNKPLVITISRQLGSGGGYIGQQLAKKLSMYYADHEIITKTA
                     EELSMLEKDLALQEEKIESFWKTFLRYNVSAPEVYIPASYNFFPTSEQLFETESEVIK
                     RIAGENSAVIMGRCGFDILHDHPNKVSVLLYCNIENRIKRIQELRHLTEEEARKTVIQ
                     SDKDRELYINSFTKKKWMDARNFDLCIDTGKISFDASVELIIAYLQSRG"
     gene            complement(3695..4534)
                     /gene="nadC"
                     /locus_tag="PKH68_04510"
     CDS             complement(3695..4534)
                     /gene="nadC"
                     /locus_tag="PKH68_04510"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNX12234.1"
                     /translation="MNKDFIEMIPAWFAEDIGDGDHTTLSCIPADAVGKSQLIIKENG
                     VLAGVEVAREIFKAFDPELKMTVFIPDGSEVKYGDIAFVVEGKIQSLLQTERLMLNIM
                     QRLSGVATRTHQYMELLKGTHTRVLDTRKTTPGLRMLEKEAVKLGGGTNHRIGLFDMI
                     LLKDNHVDFAGGIEQAITRTKEYLKAKNKDLKIEIEVRNFDELQQVMDIGGVDRIMLD
                     NFSVENTRKAVKIIGGKYETESSGGITLETLRDYAECGVDFISVGALTHSIKSLDMSF
                     KAV"
     gene            complement(4527..4925)
                     /locus_tag="PKH68_04515"
     CDS             complement(4527..4925)
                     /locus_tag="PKH68_04515"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNX12235.1"
                     /translation="MYKLVTLLFVFTVGMLQFAPAQNADVPNEITAALNSGDANKLAG
                     WLNTNVELVIGDKNDVYSKQQAIAIISDFFRNNSVQSFQVLHKGNREAASFVIGTLKT
                     TTGNYRVYVLTRKPVKQSLIQQLRIETSYE"
     gene            5116..5589
                     /gene="rlmH"
                     /locus_tag="PKH68_04520"
     CDS             5116..5589
                     /gene="rlmH"
                     /locus_tag="PKH68_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNX12236.1"
                     /translation="MKIKLLMVGKTTDSRLTALIDDYAGRLKHYVGFETIVIPELKNA
                     KSLTIEQQKEKEGALILGKLENSDHVILLDEKGKQFTSVKFSEYLANLMLNSNKCTVF
                     VVGGPYGFAEKVYTRSNAMMSLSLMTFSHQMIRLLFVEQLYRSMTIMKGEPYHHE"
     gene            5582..9220
                     /locus_tag="PKH68_04525"
     CDS             5582..9220
                     /locus_tag="PKH68_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX12237.1"
                     /translation="MNKNRAIPFRFFNRNRTLLMLLFLFAVLGSVCQWFYEQSPSNAI
                     PINDVRYNVAVKQRRAEQKMREMEALLERNSLDSLNLISFQKLTNTYLVFRGEKLIFW
                     SDNLAEQRNLKNTAWEYTLLPNMHTITRSKRVGEFNIVAYIPIKYNYPYENNELVNKF
                     TEGFKLNRDIGIVLNKPADKFAVFNNQNDYLFTLKLPDQPVYNENWAMMAMFLFLTAF
                     LLLFYLYATFPVLIGKKMLTGIEFIEVSLVMAVFVFICLKFNIPSTCFLNKFFTPYHY
                     ASNPMLASLTHLSFLTLYIYATIYLYCFHVKRSFSAKKYFLLKEIGLLMLPGLYFTLI
                     FNFLIGLVFNSTTELNILKLSDFSWASIWNHFLFLFWGISYMLLHQKLHKIILRKTGI
                     MGLLMVDLPVNLVFSIFFVIGFGKYGLEALMSFLVLSAVLYLPFFIRIFKNNKWFIAV
                     WLFFFTIFVTWNSISMNRDKKFEKYRTLAENHYLNDNADEDKIAELILADVDREIKKD
                     EIVKHLIQYPDSIQKANEYLNKVYLRGFWNKYEMRLFATQPGSVLDQTYAETIRSRGT
                     PIKSTNFYVMNSQSSDLAFLGAFRLEQAGGRQVNFFMEFYPRRYYKSYSFPGLLVESP
                     PSLQLQLGLSSARYTYHELAYSSGKFRYPRDGKWIEKKSSSFYVQNFGNYRHYIYVPN
                     IYNYFVLSEEIADGNWAYLLYVLYTFVVYLLAVFLMIWIYNLIHRKSPTTFRLTSRYL
                     YSFAALLIVSFVSIFYVTVNYMEQKYRNEQRITLERTKNYIQTSLQEKYYWTESLDSV
                     AVNTLNFNLQDLSYTFQSDIHVYDNNGVLIASSQPSLFSRGLISRRISPLPYFSKNPN
                     MNKYEYIGELEYLSAYTDFYNGDYLQIGYIAVPQYFSKDQVQLEIQEFMAVIVHIYLV
                     ITLLFILLSLIINKQLSAPLTMLENSLKDIRLGQRNKKIVYKPNDEIGQLVIQYNKMV
                     EELEKSVRLLAKSERESAWKTMARQVAHEINNPLTPMKLTIQQLQRTKKLDDNRFDDY
                     FEKSTATLIEQIENLSRIAGTFSNFARLPEAKPQKVDVAKIVTMEVGLFVHNHDRIEI
                     TYQGVENGVFVLADREQLIQVFNNLLKNAIQSIPSDRAGKIEVGLDTTTDEVHVHIRD
                     NGKGIADDIKDKLFTPNFTTKSTGMGLGLSISQNMIRIAGGEITFETEMDKGTVFHVQ
                     LPRVVEDV"
     gene            complement(9397..10233)
                     /gene="prmA"
                     /locus_tag="PKH68_04530"
     CDS             complement(9397..10233)
                     /gene="prmA"
                     /locus_tag="PKH68_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNX12238.1"
                     /translation="MKYFVVHFSFDTSSEYTADILASLLGEAGYESFINTDTGMDAYI
                     PARLFDEKTILPTLENFPFQMAINFSFEELEDKNWNEEWEKNFFEPIIIDDQCIIHSS
                     FHKNIPHLKYDIIIDPKMAFGTGHHETTSLVIGEILKMELKGKSLLDMGCGTSVLAIL
                     AAMRGAAPVTAIDIDDWCVDNSLENIRLNAVSGIEVLLGGAELLAGLHFDVVLANINR
                     NILLNDMKSYAETLAPGGELYMSGFYTEDVPVIEAKARELGFSMQYFLQKNNWAVVKM
                     KK"
     gene            10447..11844
                     /locus_tag="PKH68_04535"
     CDS             10447..11844
                     /locus_tag="PKH68_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12239.1"
                     /translation="MKRLSFLICLLIALVSSGWAQSKKIIDDIYVTPNDAVMVQEVQS
                     TQKPVRQKPTYRNGAREIVFIDQHGNRTNAVTDTVYVIDEALNDSIANVDTIEAEGEY
                     IRGFKGSSQDLEYAERIRRFHNPKYTITIADPGYNDIYFLDDNYWNVYTDGIYATITP
                     TWTNPYWFNYQFSPFSYSSWYWRSSLYSPFGWGMGGFYDPWYSPWYGGYGGYGLGWGG
                     YYGWGYPYYGGYFGMGYPYGGYYDYYGWGGYPYYGGNYYSGNNYNTRENGRVGRFNST
                     GVSGRTSATREQAVLGSGRTSGGANPYTTVSRQGTQRNRFDANATSTTGRSVQQYRNS
                     SASSGRNSYSPNVNTTTRTGAVRSADEWNATRNSNATRYSTPTRSSSGSSTVINRSGA
                     ESRSSTSSRSYESSTPIRSSSSSTPTYTPSRSSSSGSSTYSSGSSGSYSSGSSGGSSG
                     SSSSGSSRSGSGGRR"
     gene            11902..13455
                     /locus_tag="PKH68_04540"
     CDS             11902..13455
                     /locus_tag="PKH68_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12240.1"
                     /translation="MKKNILVALLAAASTLAFAQTEYDAFKLTQTELSGSARYMSMGG
                     AFGALGGDATALKDNPAGLGVYRSSEIAVTLNSMIQNTTSTWNSTQSTEDLYKTKLNS
                     FSYVMTMPRWEEKNSGLLSSNFSFTFNRLQNFNRKVSANTTGSSFSFTDFLAAFSEGY
                     SAADLSESTAYPDPYENSNNAWLSVLGYQNYLINPLADNKWSSILSEGEKVNPSTYLF
                     ETGSVSEYGIGWGGNINNNLFLGANLNMQDLSYSVNSSISESFTQGGGFDLTTALTQS
                     GVGFNLKLGAIYLPSNNLRLGVSFHTPTIYSISEYTNVKDFTTSEIDKPYLPLQGYSH
                     DYKITGPLQAQASAAYLFGKKGLISVEYNYINYPGMRLGLENGDTKYYQDENRNMSNV
                     FIAGQNLKFGAEYKLNPSFALRAGYALMKSPANAGYLQGKSLKLNSANTNTEYFNQFD
                     TKYLTAGAGYRAAGWFLDLAYAQKIQDEDMYPYQVLRYTGNQVTNIVTPAAVNTTTHN
                     IVVTLGIKL"
     assembly_gap    13550..13649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13654..>15185
                     /locus_tag="PKH68_04545"
     CDS             13654..>15185
                     /locus_tag="PKH68_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNX12241.1"
                     /translation="MSLQSEINRRRTFAIISHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIRKTATSDWMEIEKQRGISVATSVMGFEYRDYKINILDTPGHQDFAEDTFRTLTA
                     VDSVIIVVDAAKGVEAQTRKLMNVCRMRNTPIIVFVNKMDREGRDPFDLMDELESELG
                     IHVRPLSWPISMGATFKGVYNIYKQNLQLFTPNKQFISESVELANIDSKELDHLVEKK
                     LADKLREDMELINGVYSSFDSELYLQGHLAPVFFGSALNNFGVKELLDCFVEVAPSPR
                     PVKAVERVVNPYEEAFSGFVFKIHANMDPNHRSCIAFVKICSGMFERNVNYKHVRNGK
                     MMRFSSPTAFMAQKKETVDEAYAGDIVGLPDTGNFKIGDTLTGGEELHFKGIPSFSPE
                     MFMYLENADPMKTKQLEKGINQLMDEGVAQLFVNQFNNRKIVGTVGQLQFEVIQYRLL
                     HEYNASCRWEPVSLYKACWIESDDAAELDNFKKRKVQYMAKDKDDRDVFLADSNYVLQ
                     MA"
BASE COUNT         4230 a         3236 c         3296 g         4323 t
ORIGIN      
        1 attttaaaca aataatttac ataaataaca ttgaaaaatg ctcactaatt cccccttcag
       61 ggggttaggg ggcagctttt tatcgtatga aaatactaat agcaaccgat aaaccatttg
      121 ccaaagttgc agtggacgga attcgcaatg aaatagaagg agtgggttat gaactggctt
      181 tgctggagaa atataccgaa aaacagcaat tgctggacgc catagccgat gccgatgccg
      241 tgattatccg cagcgacatt gtggatgagg aggtaatccg ggccggaaaa aaactgaaaa
      301 tagtggttcg tgccggagcc ggatacgaca atgtggatct ggaagcagct accaaagccg
      361 gagtgtgtgt gatgaatacg cccggacaaa acgccaatgc tgtggcagaa ctcgttttcg
      421 gattactggt ttatgccgta cgtaactttt acaacggaac ttccggtacc gaactgatgg
      481 gtaaaaagct gggtattcat gcttatggaa atgtcggacg gaatgtcgcc cgcatcgcca
      541 aagggtttgg aatggaagtt ttggcttacg atgcctattg ccgcgccgaa gatattaagg
      601 ctgaaggtgt caatccaacc tgttcggtta aaacgcttta tcagggtagc gatatcgttt
      661 cggttcacat tccgctgact gccgaaacgc gacagattat caataaggaa ttgcttgaat
      721 tattacctaa aaacgcggta atggtgaata cagcgcgcaa agaggtgata aatgaagcgg
      781 atctactgca gcacatgatc gataatccga aattcaaata tgtaacggat gtagcttccg
      841 ataatgaagc cgaaatgaag gaaaaagcag gagaacagta tttttccact gccaaaaaaa
      901 tgggcgctca aactgctgaa gccaatgtga atgctggtat tgctgctgct cagcagattg
      961 ttgactttct gaaaaacgga aatgaaaaat atcgcgtaaa taaatagtta tcaacatata
     1021 attccataaa aaaaggattt cattttttcg aaatcctttt ttttgcttgt acagttcaat
     1081 gtcaagttct ggtaagattt tttttgaagt aataataaaa cggttttttt tgcatcttat
     1141 taaaatccct gattgaggaa ttatttataa ccatatgttc taaaagtccc cctttggggg
     1201 atttagggcg cttttttacc caattccttc gtactcaaag cccatttctt tcatttcagt
     1261 tttatcatag atatttctac cgtcgagaac caatggattt ttcatcgatt ttttaatcac
     1321 attccagctt ggcatgcgga attctttcca ttcggtcatt agcagcaaag catcggcttc
     1381 gtcggctgca tcgtaaatgt cattggcaaa atataccttc gaaaaatcaa gtttttctgc
     1441 ttcgggaaaa ttcttttgtt ctttttccag aaattcaatt cgttctttgg aagcaggcat
     1501 ggctatcgga tcaaacacac gcaccgttgc acctgcccta aggagttttt ctatcaccac
     1561 aaaagccggc gcctcgcgta tgtcatcggt gttgggcttg aatgctaatc cccagattgc
     1621 caccttttta ccgttcatct ccattcccca gcgtttggtc agttttctga aaagaacttc
     1681 tttctggatg ttatttacat cttccactgc gcggagcagg cggagttcgt agccgttttc
     1741 ctgcgcggtg cggatgagtg cctgcacatc tttgggaaag cacgagccgc cgtaacccga
     1801 accggcatac aaaaattttc cgccaatacg gacatctgat ccgatacctt tgcgcaccat
     1861 attgatgtcg gcacccacaa tttcgcacag gttagcaatg tcgttcataa aactgatacg
     1921 ggttgccagc atggaattgg cagcatattt ggtcatttcg gccgaaggaa tatccatgaa
     1981 aataacgcgg aaattattga gcaggaacgg acggtacaat ttggtaagca attctttggc
     2041 tttttcgctt tcggtaccca ccaccactcg gtcagggctc ataaagtcgt tgattgcatc
     2101 gccctctttc agaaattcgg gattggaagc cacatcaaac ggaatactga caccccgtag
     2161 gtcaagttct gcctgtatgg tttgtttcac cagattggct gtgcccacgg gaacggtact
     2221 tttagtaacc agcagcacgt agttgttcat tttccggccc acttcggcgg ccacctgcaa
     2281 tacgtatttc agatcggcgc tgccgtcttc gttgggtgga gtgcccaccg cgctgaaaat
     2341 cacctgtact tcgtccagca tatcttccag ccgggtggta aagtgcaacc gttcggcttt
     2401 cacgtttcgc tgcaccatgt cctccagtcc gggttcgtaa atgggaataa tgcctttcag
     2461 gagattgtca atcttttgtt tgttgacatc cacgcaggtt acatctacgc ccatttcggc
     2521 aaaacaggct ccggaaacca gcccgacata gccggtgcca ataatagcta ttttcatgtg
     2581 tttgtaattt atatatctgc cccctaaccc cctaaagggg gaatattgag cgtctaacat
     2641 ttgttgtgag tttttcaatc atgcccttgt gttaaaaata ttttacttgg acatgtgtgt
     2701 ttcattttct tgttttcaat gatgaataaa acaaaagagt tactttcaga ttgtaactct
     2761 ttgcaaatat cggtaatttt cgggtaaata caaaaaaagc ttgttcaact ctttttttcg
     2821 gtataatagc ttaacattcg ataaacagcc gccagttgct gcgggtgagc ggctcaaaaa
     2881 tgagttgatt tataaattcg ggatcggagt aactcatcct cttgactgca ggtaagcgat
     2941 gataagttcc acactggcat cgaaactgat tttgcccgta tcaatacaaa ggtcaaaatt
     3001 acgggcatcc atccatttct ttttggtgaa gctgttgatg tacaattccc ggtccttatc
     3061 actttgaatg actgttttgc gggcttcttc ctcggtgagg tgacggagtt cctgaatacg
     3121 ttttatccgg ttttcaatgt tacaatacag taaaacactg actttattcg gatggtcatg
     3181 caaaatgtca aaaccgcacc ggcccataat aacggcggaa ttttcaccgg caatgcgttt
     3241 tataacttct gattctgttt caaaaagctg ttcggaagtg ggaaaaaagt tgtaggatgc
     3301 aggaatataa acttcgggag ccgaaacatt gtaacgcagg aacgttttcc agaaagattc
     3361 aattttttct tcctgcaaag ccaggtcttt ttcaagcatc gaaagttctt ctgcagtttt
     3421 tgtaattatt tcatggtcag cataatacat actgagtttt tttgccagtt gctgaccaat
     3481 atatccacca ccactaccaa gctgccggct gatggtaatg accaacggtt tgttttgttc
     3541 catttttttg cctcctgatg gttagattag ttaggtaaaa tatttccgta taattataac
     3601 atttcagaaa cgaaaattcc tataccgttt atcaaaaaaa aaccgtctcc aaaacaggaa
     3661 acggtttttt ttgttaggca gatttatttt aatattatac agccttaaag ctcatatcca
     3721 gacttttaat ggaatgtgtc agtgcgccta ccgagataaa atccacacca cattcggcat
     3781 agtcgcgcag ggtttcgagg gtgatgcctc ccgatgactc tgtttcatat ttgcccccaa
     3841 ttatttttac tgcttttcgc gtattttcaa cactgaaatt gtcgagcata atgcgatcaa
     3901 cgcctccaat atccattact tgctgtaact catcgaagtt acgtacttct atttcaattt
     3961 tcagatcttt gtttttggct ttcaggtatt cctttgtccg ggtaatggct tgttcgatgc
     4021 cgcctgcaaa atccacgtga ttgtccttga gcagtatcat gtcaaacagt ccgatgcggt
     4081 gattggtgcc gccaccgagt tttacggctt ccttttcgag catgcgcaat cccggagtgg
     4141 tttttcgggt gtcgagcaca cgggtatggg tgcctttgag cagttccata tattggtgtg
     4201 tgcgggtggc cacgccgctc agccgttgca tgatattgag catcagccgt tcggtttgta
     4261 acagcgactg gatttttcct tccactacga atgcaatatc accgtatttt acttccgatc
     4321 catcggggat aaaaacggtc attttcagtt cggggtcaaa ggctttgaat atctcacggg
     4381 ctacttccac gcctgccagt actccgtttt ctttgataat cagttgtgat ttacctactg
     4441 cgtcagcagg aatacacgat agggtggtgt ggtcaccatc tccgatatcc tcggcaaacc
     4501 atgccgggat catttcgata aaatctttat tcataggatg tttctatacg taattgctgt
     4561 attaaggatt gtttcaccgg tttgcgggtc aatacatata cccggtaatt cccggtcgta
     4621 gttttcaggg tgccaatcac aaaactggct gcttcgcggt tacctttgtg caacacctgg
     4681 aagctttgta ccgaattatt tctgaagaaa tcggagataa tggctatagc ctgttgtttg
     4741 ctgtaaacat catttttatc gccaataacc aattccacgt tggtgttgag ccaccctgcg
     4801 agtttattgg catcgccgct gttgagggct gcggttattt cgttgggtac atcggcattc
     4861 tgagcgggag caaattgaag cattcccacg gtgaatacga agagtagtgt tactaattta
     4921 tacattttca ttttattgaa ttcttttttg gaaataatta gccggttaaa cggtttttct
     4981 tctgtttttt ttgcaatatt tgcattggaa aataaatccg cgtacaaagt tattaaaatt
     5041 tcggtgcttg gtgaacggat tttctcaatt attcctacaa atagcagacc aaaattgtaa
     5101 aaaaaggaaa atcaggtgaa aataaaattg ttaatggtcg gaaaaacaac agacagccgg
     5161 cttactgcgt tgatagatga ttacgccggt aggttgaaac actatgtagg ttttgaaacg
     5221 atcgtaatac ctgaactcaa aaatgcaaaa agtttaacaa tagagcagca aaaggaaaaa
     5281 gagggcgcac tgatacttgg taagctcgaa aactccgatc atgttatcct gctcgacgaa
     5341 aagggtaaac agtttacttc tgtcaaattt tcggaatacc tggctaatct gatgctgaat
     5401 tctaataaat gtacggtttt tgtagtagga ggaccgtatg gctttgccga aaaggtatat
     5461 acccgcagca atgctatgat gtcgctttcg ctgatgactt tttcgcacca aatgatacgg
     5521 ctgctctttg tggagcaact ttaccgatcc atgaccatta tgaaaggaga accttatcat
     5581 catgaataag aacagagcaa tccctttccg gtttttcaac cgaaacagaa cattactgat
     5641 gttattgttt ctgtttgctg tgctcggtag tgtgtgtcag tggttttatg aacaatcgcc
     5701 ctccaatgcc attcccatca atgacgtcag atataatgtg gcagtgaagc agcgcagagc
     5761 tgaacagaaa atgagggaga tggaagcact gctggagcga aattcgctgg actctctcaa
     5821 cctgatttct tttcagaaac taaccaatac atacctcgtt ttcaggggag agaaactgat
     5881 attctggtcg gataatctgg ccgaacagcg gaatctgaaa aatactgcct gggaatatac
     5941 attgctgccc aatatgcata ccattaccag gtctaagcgg gtgggtgaat ttaatattgt
     6001 ggcatatatt cccatcaaat acaattatcc ctacgaaaat aatgaactcg tcaataaatt
     6061 taccgaaggt tttaagctca acagggacat aggcattgta ctcaataaac ccgcggataa
     6121 atttgccgtt tttaataatc agaacgatta tctttttacc cttaagctgc ccgaccagcc
     6181 cgtttacaac gaaaactggg ccatgatggc tatgtttctt ttcttaactg cttttttatt
     6241 gttgttttat ttatacgcta cctttccggt gctgattggc aaaaagatgc tcacaggcat
     6301 cgaatttata gaagtaagcc ttgtcatggc cgtttttgtg ttcatctgtt taaaatttaa
     6361 tattccttca acctgttttc tcaataagtt tttcactccc tatcattatg cctccaaccc
     6421 gatgctggca tcgttgacac acctcagctt tctgaccctt tacatatatg ccaccattta
     6481 tctctattgt tttcatgtaa aacgaagttt ttctgcgaaa aaatatttcc ttttaaaaga
     6541 aattggatta ttgatgctgc cggggctata tttcaccctg atattcaatt ttctgatcgg
     6601 tctggtattt aattccacta ccgaactcaa tatcctgaaa ctttcggatt tcagctgggc
     6661 gtctatctgg aatcattttt tattcctgtt ttggggtatc agctacatgc tgcttcatca
     6721 gaaactgcat aagattattc ttcgtaaaac aggaattatg gggttgctca tggtggattt
     6781 gcctgtgaac cttgtcttca gcattttctt tgttatcggg tttggtaaat atggattgga
     6841 agcgttaatg tctttccttg ttctttcggc tgttttatac cttccttttt tcatcagaat
     6901 atttaagaac aataagtggt ttattgctgt ctggttgttt ttctttacca tttttgtaac
     6961 ctggaattca atcagtatga accgtgacaa aaagtttgag aaatacagaa ccctggccga
     7021 aaatcactac ctcaatgaca atgccgatga agataaaata gccgaactca tccttgccga
     7081 cgttgatcgg gaaattaaga aagatgaaat tgtaaagcac ctgatacaat atccggactc
     7141 cattcagaag gcaaacgaat atctcaataa ggtgtatctg agaggatttt ggaataaata
     7201 tgagatgagg ctgtttgcta cacagccggg ctcggtgttg gaccagacct atgccgaaac
     7261 tatccggtcg cggggtacac cgataaaatc gaccaacttt tacgtgatga acagtcagtc
     7321 gtccgatctt gcttttttgg gagcattcag actggagcag gccggaggca ggcaggtgaa
     7381 tttctttatg gagttttatc cccgcaggta ttataaaagc tacagttttc ccggattgct
     7441 ggtggagtct ccgcccagtc tgcaacttca gctcggactc tcgagtgcgc gttacacgta
     7501 ccacgagctg gcttactcct cgggaaaatt ccggtatcca cgggatggaa aatggataga
     7561 aaagaaatcg tccagcttct atgttcagaa ttttggcaat taccgccatt atatctatgt
     7621 gcctaatatc tacaattatt ttgtgttatc tgaagaaatt gccgatggta attgggcgta
     7681 cctgctctat gttttataca cctttgtggt atatttgcta gcggtttttt taatgatttg
     7741 gatatacaat ctcattcacc ggaagtcgcc caccacgttc aggctcactt ccaggtatct
     7801 ttattctttt gccgcactgc tcattgtcag ttttgtttca atattttatg ttacagttaa
     7861 ttatatggaa cagaagtatc gcaacgaaca gcgaataacg ctcgaacgaa ctaaaaacta
     7921 cattcagaca tcgcttcagg aaaaatacta ctggaccgaa agtctggact ctgtagcggt
     7981 taataccctc aattttaatt tgcaggattt gtcctacacc tttcagagtg atattcatgt
     8041 gtatgacaac aacggggtgc taatagccag ttcgcaaccc agtctgtttt cacgcggact
     8101 catcagcagg cgcatttcgc cgttacccta cttctccaaa aatcccaaca tgaataaata
     8161 tgagtacatt ggcgagttgg aatacctttc ggcctatacc gatttttaca acggtgatta
     8221 tcttcagatt ggctatatag ctgtgcctca atacttcagc aaggatcagg tgcaactgga
     8281 gattcaggaa ttcatggcag ttattgtaca tatatatctt gttatcaccc tgctgtttat
     8341 ccttcttagt ctgattatca acaagcagct ttcggcgccg ctcaccatgc ttgaaaatag
     8401 cctgaaagat atcagactcg ggcaacgcaa caaaaaaata gtgtataagc ccaacgatga
     8461 aataggtcaa ctggtgattc agtacaataa aatggtggaa gaacttgaaa aaagtgttcg
     8521 gttgttggct aaatcggaac gcgaatcggc ctggaaaacg atggcacggc aggtggcaca
     8581 cgaaatcaat aatccgctta cacccatgaa actgaccatt caacagttgc aacgcactaa
     8641 aaaactcgat gataaccgat tcgatgatta ttttgaaaaa tcgacagcta cactcatcga
     8701 gcagattgaa aacttgtcgc ggattgccgg aacattctcc aattttgccc ggttgcccga
     8761 agcaaaaccc caaaaagtgg acgttgcaaa aatcgtaacc atggaggtgg ggctttttgt
     8821 acacaatcat gaccgtattg aaatcacgta tcagggagtt gagaatggag tatttgtcct
     8881 ggctgaccgc gaacaactca ttcaggtgtt taataatctg ctgaaaaatg ccattcaatc
     8941 catcccgtca gaccgggccg gaaaaattga ggtggggctg gacacaacaa cagatgaggt
     9001 tcacgtgcat attcgtgata acggaaaagg gatagccgat gatatcaaag acaaattatt
     9061 cacccccaac tttaccacca aatccaccgg tatgggcctg ggactttcca tcagccagaa
     9121 catgatacgc attgcaggag gcgaaattac ttttgaaacc gaaatggata aaggaaccgt
     9181 ttttcacgtt cagcttccaa gagtagttga ggatgtttga tgtttaatgt ttaatgttta
     9241 atgtgtagtt gtttaatgtt taattgtcga attttataat cgatgaaaca cccaatattt
     9301 taataaccaa taacacaata accaatagct taataaccaa taacttaata accaataact
     9361 ttcttttctt tactccccct tcagggggct ggggggctac ttcttcattt tcaccaccgc
     9421 ccagttattt ttctgcagaa agtactgcat actgaagccg agttcacgtg cttttgcttc
     9481 aattaccggc acatcttcgg tgtaaaatcc gctcatgtaa agttccccgc ccggtgcaag
     9541 tgtttccgcg tatgatttca tgtcgttcag tagaatgttg cggttgatat tggcaagcac
     9601 cacatcgaaa tgaagtcccg caagcaattc agctccaccg agaagtacct caattccgct
     9661 gacagcattc aaccggatgt tttccagtga attatccaca caccaatcat caatatcgat
     9721 agctgtaaca ggagcggcac cccgcatagc agccagaata gccagtaccg atgttccaca
     9781 gcccatgtca agcaatgatt tgcccttcag ctccatcttc agaatttcac ctatcacgag
     9841 gctggtagtt tcgtgatggc cggtgccgaa tgccattttc gggtcaataa tgatgtcgta
     9901 tttcaggtga ggtatgttct tgtgaaacga actgtggata atgcactgat cgtcaatgat
     9961 aatcggttcg aagaaatttt tttcccattc ctcgttccag tttttatcct caagttcttc
    10021 aaatgagaaa ttaatcgcca tctgaaacgg aaaattttca agggtcggaa gaatggtttt
    10081 ttcgtcaaag agtctggccg ggatgtatgc atccatgccg gtatcggtgt taataaaact
    10141 ctcataaccg gcttcgccaa gcagggaagc cagaatatcg gcagtatatt cagaggaggt
    10201 atcgaaggag aaatgaacga caaagtattt cataaagcat aatttttttt cgaaagtaat
    10261 gagttgattt tttgcaaaac aaaagtacaa aataatgttg attggatgcc ccaatttcag
    10321 tatatttgcg acgactgaaa ttttaaatgt taatgtgctc gccaacaaac ggaatggcac
    10381 aattgttgta caattacaaa tgaaatagta caacgaaatt caacaaaacc tttaattaaa
    10441 aataaaatga aacgcttaag ctttttaatt tgtctcctga tagcactggt ttcatccggg
    10501 tgggctcagt ccaagaagat tattgacgat atttatgtca cccccaatga tgctgtcatg
    10561 gttcaggaag tacaatcgac tcaaaaacct gttcgtcaga aaccgaccta cagaaacgga
    10621 gcccgggaaa tcgttttcat cgaccagcac ggtaaccgca ccaatgcggt gaccgacacc
    10681 gtgtatgtga ttgacgaagc gctcaacgat tcgattgcca atgtggatac aattgaagcc
    10741 gaaggtgaat atatccgcgg tttcaaaggc tcgagccagg atctggagta tgctgaacgt
    10801 attcgccgct tccacaatcc gaaatatacc atcaccattg ccgatcccgg ttacaacgac
    10861 atttattttc tggatgataa ttactggaat gtatatactg atggaattta tgctaccatt
    10921 actccgacgt ggaccaatcc gtactggttc aattatcagt tttcaccgtt cagctacagc
    10981 agctggtact ggcgaagcag cctgtactca cctttcggat ggggaatggg cggattttat
    11041 gatccatggt attcgccctg gtacggtggc tatggtggct atggtttagg ttggggaggt
    11101 tattatggct ggggatatcc atattacgga ggctatttcg gcatgggtta cccttacgga
    11161 ggctactatg attattacgg ctggggagga tatccctact atggcggaaa ttactattca
    11221 ggcaataatt acaatacccg tgaaaacgga cgtgtaggac gatttaattc tacaggtgtt
    11281 tcaggtagaa catcggctac acgtgagcaa gctgtcctcg gttcgggacg tacaagtggt
    11341 ggtgcaaatc cgtataccac tgttagccgt cagggaacac aacgaaatcg ttttgatgcc
    11401 aatgccactt caactaccgg acgcagtgta cagcaataca gaaattcatc tgcatcctca
    11461 ggtcgcaact cctattcgcc caatgtgaat acaacgaccc gcaccggggc ggtaagatcg
    11521 gcggacgaat ggaatgccac ccgcaacagc aacgccaccc gttattccac cccaacgcgg
    11581 agttcatcgg gatcatcaac ggtgattaac cgctcgggtg cagaaagcag aagctccacc
    11641 agcagccgta gttacgaaag tagtacgccg atacgctcaa gcagttcatc cacaccgact
    11701 tatactccca gcagaagcag tagctcaggc agcagcactt attcatcggg aagttcgggt
    11761 tcatacagca gtggcagcag tggcggaagc agcggaagca gcagttcggg cagcagtcga
    11821 tcgggtagtg gcggacgccg gtaaaccgat attatcccgt aattaaaact tattattatt
    11881 tatccataag attattataa aatgaaaaaa aatattttgg ttgcactgtt agccgccgca
    11941 agtacgttgg cttttgctca gaccgaatac gatgcattta aactgacaca aactgaattg
    12001 agcggttcgg cacgctatat gagtatggga ggtgcattcg gtgcactcgg cggcgatgct
    12061 acggcattga aggacaatcc ggccggactg ggtgtttatc gtagttccga aattgcagtt
    12121 acactcaatt caatgattca gaatacaact tctacatgga atagcacaca atcaacagag
    12181 gatttgtata aaaccaaact gaacagtttc tcttatgtga tgaccatgcc gcgttgggaa
    12241 gagaaaaaca gtggattgtt gagttccaat ttctcattta ctttcaacag gctgcagaat
    12301 ttcaaccgta aagtgagtgc taacacaaca ggaagttcct tttcgttcac cgattttctg
    12361 gctgcatttt ccgaaggata cagtgctgcg gatttatccg aaagcaccgc ctatcccgat
    12421 ccctatgaaa attcaaacaa tgcctggctt tcggttctgg gataccagaa ttacctgatc
    12481 aatccattgg ccgacaataa atggtcgtct atcctgagtg agggtgagaa ggttaatcca
    12541 tcaacttacc tctttgaaac cggttcggtg agcgaatacg gtattggttg gggtggcaat
    12601 attaataata acctctttct gggtgccaat ctgaatatgc aggacttgtc ttacagtgta
    12661 aattcaagca tttcagaaag ttttactcag ggtggcggat ttgatctgac cacggccctg
    12721 acgcaatccg gagtgggttt taatctcaaa cttggtgcca tttatctgcc ttccaacaat
    12781 ctgcgcctgg gtgtgtcatt ccacacacct accatttata gtatttcgga atataccaat
    12841 gtgaaagatt tcaccacttc tgaaattgac aaaccttatc tgccactgca gggttacagc
    12901 cacgattaca aaattactgg tccactgcag gctcaggcaa gtgccgctta tctttttggc
    12961 aagaaaggtt taatctcggt ggaatataac tacatcaatt atcccggaat gcgacttgga
    13021 ttggagaatg gggataccaa gtactatcag gacgaaaacc ggaatatgtc gaatgtcttt
    13081 attgccggtc agaacctgaa attcggagct gaatataaac tgaatcctag ttttgccctc
    13141 cgtgccggct atgcattgat gaaaagtcct gcaaatgccg gttatctgca gggaaaatca
    13201 ttgaaactca attcagcaaa taccaatacc gaatatttca atcagtttga tacaaagtat
    13261 ctgaccgcag gggccggata ccgcgctgca ggttggttcc tcgacctggc ttatgctcag
    13321 aaaatacagg acgaagatat gtatccatac caggtgctcc gttacaccgg aaatcaggtt
    13381 acaaatatcg taactccggc agctgtcaat actactacac acaatatagt agttacactg
    13441 ggtataaaat tgtaatcaaa taccgattca ttaacaaaaa agccagttct gcgggactgg
    13501 tttttttgtg tattcacgtg aaatacacta cctttgccgt ccgaaaaagn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt aaaatgtcat tacaatccga aataaaccgc
    13681 cgcagaacct ttgccatcat cagtcacccc gatgcgggga aaactaccct taccgaaaaa
    13741 ctcttgcttt tcgggggcgc tattcacatt gcgggtgcgg taaaatcaaa taagatacga
    13801 aaaacagcca cttccgactg gatggaaatc gaaaagcagc gtggaatttc ggtggctacc
    13861 tcggtgatgg gtttcgaata ccgtgattac aaaatcaata ttctcgatac acccggtcac
    13921 caggactttg ccgaagatac tttccgtaca ctcacagcgg tggatagtgt gatcattgtg
    13981 gtggacgctg ccaaaggagt ggaagcgcag acccgtaaac tgatgaacgt gtgccgtatg
    14041 cgtaatacgc ccatcattgt gtttgtgaat aagatggacc gcgaaggtcg cgaccccttt
    14101 gacctgatgg atgaacttga atcggaactg ggcatacatg tgcgaccgct cagctggcct
    14161 atcagtatgg gagccacatt caaaggagtg tacaatatct ataaacaaaa cctccagttg
    14221 tttacaccca acaagcaatt tatcagcgaa tcggtggaac tggccaatat cgacagcaaa
    14281 gagctggatc acctggtgga aaagaaactg gctgataaac tccgtgagga catggaactg
    14341 atcaatggtg tctattcgag ctttgactcc gagttgtacc tgcagggaca tcttgctccg
    14401 gttttttttg gaagtgcgct gaataatttc ggggtgaagg agctgctcga ttgttttgtg
    14461 gaagtagcgc cctcgccgcg gccggtaaag gcagtcgaac gggtggtcaa tccttacgaa
    14521 gaagcatttt cagggtttgt atttaagata catgccaata tggaccccaa tcaccgcagc
    14581 tgcattgctt ttgtgaagat ttgctccggg atgtttgaac gaaatgtgaa ctataagcat
    14641 gtgcgcaatg gcaaaatgat gcgtttttcc agtcctactg cttttatggc tcagaaaaaa
    14701 gaaacggtgg acgaggctta tgcgggtgat attgtagggt tgcccgatac cggaaacttc
    14761 aaaatcgggg ataccctgac cggtggtgaa gagctgcatt tcaagggaat tccgagtttc
    14821 tcgcctgaaa tgtttatgta cctggaaaat gccgacccga tgaaaaccaa acagctcgaa
    14881 aaaggaatta atcagctgat ggacgaaggg gtagcccagc tatttgtcaa tcagttcaac
    14941 aaccggaaaa ttgttgggac agtaggtcag cttcagtttg aggtgataca gtaccgactg
    15001 cttcacgaat acaatgccag ttgccgctgg gaaccggtat cactctacaa ggcctgctgg
    15061 atagaatcgg atgatgccgc cgaattagat aatttcaaaa aacgaaaagt gcagtatatg
    15121 gctaaagata aggacgatcg cgatgtattt ctggccgact ccaactatgt actacagatg
    15181 gcaca
//
LOCUS       DAOPZK010000049        14081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_4716_length_14081_cov_21.520818, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000049 DAOPZK010000000
VERSION     DAOPZK010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14081
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4716_length_14081_cov_21.520818"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKH68_04550"
     CDS             complement(<1..313)
                     /locus_tag="PKH68_04550"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNX12242.1"
                     /translation="MKTKEEIVQNWLPRYTKRPLEEFTEYILLTNFSNYVEMFASWFN
                     VPVLGRDGNMINASADGITIINFGMGSPNAAMIMDILSAIHPRAVLFLGKCGGLRDKN
                     KL"
     gene            403..867
                     /locus_tag="PKH68_04555"
     CDS             403..867
                     /locus_tag="PKH68_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNX12243.1"
                     /translation="MWQLNLPEYQFRLKQTEKGYFVLDNFRKRFVKLTPEEWVRQNFL
                     RFLTDNKHFPSALIAVEKQLTMNGMKKRCDAILYDIQANPVMIIEFKAPNITINQQVF
                     DQVAVYNAKLKVNYFLISNGMEHFCCKVNVENASYSFFEGIPEYEDFCSLTT"
     gene            complement(902..1558)
                     /locus_tag="PKH68_04560"
     CDS             complement(902..1558)
                     /locus_tag="PKH68_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNX12244.1"
                     /translation="MEVDEVIVELKKLATADHLSKLSHFGIPGDKALGIKLPDLRKLA
                     KRIGKNQVLGFELWQTDIHEAKLLATMIMDAQLLTNSQYDSLVKDFDSWDMCDCACKM
                     LQGAAFARNKIDEYAIRSEEFVKRTAFVLMCQFAVHDKKKPDDFFYPMLQIIEREAWD
                     ERNFVRKAVNWALRQIGKRNENLRRKAIETAKRILAQNTKSARWIAKDALRELKKKAP
                     "
     gene            complement(1638..1799)
                     /locus_tag="PKH68_04565"
     CDS             complement(1638..1799)
                     /locus_tag="PKH68_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12245.1"
                     /translation="MADLEKTIPYSEASFATEPTKWTKFWRTSILFQLIRFFVLNLKI
                     MRIVVGGHS"
     gene            complement(1842..3299)
                     /locus_tag="PKH68_04570"
     CDS             complement(1842..3299)
                     /locus_tag="PKH68_04570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNX12246.1"
                     /translation="MARVLILGAGISGHTAALIARRKLGKSHEVVVVSPNSNYQWVPS
                     NIWVGIGLMTTKQVIFPLAPVYKKQGIIFKQAKALSIHPEGDDTNVKQYVVIQYVAGD
                     NKEQTEKVEYDYLINATGPKLNFAATEGLGPDLNSFSVCTYDHAAHTWENLQKCFARM
                     EKGEKQRFLIGTGNGMATCQGAAFEYALNIAFEIKRRKLLDLAEIIWISNEYELGDFG
                     MGGAYVKQGGYVTPTKIFAESIFKEYGIKWIVGSAVQKVEPGVVSYENLAGEYHKLEF
                     DFAMLIPAFAGVGLKAFNKKMEDITAQMFAPNGLMKVDADYTPKPFDEWKASDWPKTY
                     NSPLYDNIFAAGIAFAPPHTISKPMTSKNGTSITATAPRTGMPSGVMGRIVALNITEQ
                     IKSGNKELKHEASMAKMGAACIVSAGYGIRHGKAAVMTVFPIVQDWEKFPKWGRNIGY
                     TVGEIGLAGHWMKLFMHYMFIYKAKALPLWWMIPE"
     gene            complement(3565..4476)
                     /locus_tag="PKH68_04575"
     CDS             complement(3565..4476)
                     /locus_tag="PKH68_04575"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNX12247.1"
                     /translation="MADLTTQLGKLQMKNPVMTASGTFGYGTEYSDFIDLSRIGGIIV
                     KGTTLRERQGNQYPRMAETPSGMLNAVGLQNKGIDYFVNHIYPTLKDIDTNVMINLSG
                     STIEDYIKTAEIINTLDKIPGIELNISCPNVKEGGMAFGVSCLAASQVVAEVRKVYHK
                     ELMVKLSPNVTDIAEIAKGVEGAGADSISLINTLLGMAIDAKTRKPVLSTITGGLSGP
                     AVKPIALRMVWQVAKAVKVPVIGLGGIMNATDAIEFMLAGATAIQVGTANFIDPAISV
                     KIVEGIDNYLNKNGFSSAKDIIGALEI"
     gene            complement(4464..5240)
                     /locus_tag="PKH68_04580"
     CDS             complement(4464..5240)
                     /locus_tag="PKH68_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNX12248.1"
                     /translation="MKKFILDLTITHKVQLNEQNVLLKLTTIDQLPEILPGQFAEIRV
                     DGSPTTFLRRPISINFVDYEKNELWLLIQIVGDGTRRMADMEVGQTLNAILPLGNTFT
                     MPAEKNARIVLIGGGVGIAPLLMFGEKLHSMGYNCNFLLGARSKTDLLQLDEFERYGK
                     VYTTTEDGSYGEKGYVTHHSLLIGSNFDYIYTCGPTPMMKAVASYAAINGVFCEVSLE
                     NMMGCGIGACLCCVTDTTEGNLCVCTEGPIFNITKLKWQI"
     gene            complement(5369..5836)
                     /locus_tag="PKH68_04585"
     CDS             complement(5369..5836)
                     /locus_tag="PKH68_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX12249.1"
                     /translation="METTEKERIEKVMQAEGLNATQFASEIGIKTPTLSHILSGRNNP
                     SLDVLKKILDRYRTINSDWLILGIGQMYRDSKKSQTPTLFENMDEIDTKSAGYGRNIV
                     EKNVTEESISYIKPVADSSQTIKIPPVTTDKSKFIKKIIVYYDDNTFQEFEAK"
     gene            complement(6922..7695)
                     /locus_tag="PKH68_04590"
     CDS             complement(6922..7695)
                     /locus_tag="PKH68_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNX12250.1"
                     /translation="MATVTKTDKEITVEEKLKALYELQKVDSEIDQIKILRGDLPLEV
                     QDMEDEIVGLSTRLENFENEIAELTTAIKGKKVEIEEAKIKIERYKEQQNNVRNNREY
                     DNLSKEIEFQTLEIELSEKRIREFTANLENKKIEKDSTFNQLSERKLDLEQKKGELDE
                     IVSETKQEEEKLREHAKKIEAKIEARLVSAFKRIRKNARNGLAVVSVQRDACGGCFNK
                     IPAQRQLDIRSRKKIIVCEYCGRILVDPEIGGEQNPEVK"
     gene            complement(7699..8796)
                     /locus_tag="PKH68_04595"
     CDS             complement(7699..8796)
                     /locus_tag="PKH68_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNX12251.1"
                     /translation="MTTVRTITQWIEEAAPLALQEEYDNAGLLIGSPDMLVTGVLLTV
                     DVTEDIVDEAIQNGCNLIISHHPLIFRGIKNITGQNDVQRCVLKAIKNEIAVYAAHTN
                     MDNVMEGVNGKIADKIGLKNRQILQPKKELLLKLVTFVPADFIQQVRNALFEAGAGSI
                     GNYDNCSYMSDGRGTFRANEKANPFVGEIQIEHTESEIRLEVIFPEYLKVKITNALLR
                     SHPYEEPAYDFIALQNTWNSVGAGIVGELPAETDEMEFLHQLKSVFHLQTLRHTSLLN
                     RKIRKIAVCGGSGSDLTRQAIAVGADVFISADFKYHEFFDAKNKILIVDIGHYESEQF
                     TKDVFYEIITKKIPKFAVRISDVNTNPIKYL"
     gene            complement(8973..9365)
                     /locus_tag="PKH68_04600"
                     /pseudo
     CDS             complement(8973..9365)
                     /locus_tag="PKH68_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
     gene            complement(9524..10240)
                     /gene="hisA"
                     /locus_tag="PKH68_04605"
     CDS             complement(9524..10240)
                     /gene="hisA"
                     /locus_tag="PKH68_04605"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNX12252.1"
                     /translation="MEIIPAIDLIDGKCVRLTRGDYERKKIYNEDPLEVAKMFAGAGI
                     TRLHLVDLDGAKAQHIVNHKVLERICSFTNLIVDFGGGLKSDKDLQIAFECGASMITG
                     GSIAVKNPDIFNNWINSFGSEKIILGADVMDEKVAVTGWTETTDRELIPFLKNYIDKG
                     ITKIICTDISKDGMLEGPAFDLYEKILGEFPQIYLIASGGVSSMADIEKLNNANVPAV
                     ILGKAIYEGKVKLNELSVFF"
     assembly_gap    10243..10342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10343..12129)
                     /locus_tag="PKH68_04610"
     CDS             complement(<10343..12129)
                     /locus_tag="PKH68_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNX12253.1"
                     /translation="MCGIAMVRLLKPLEYYQEKYGTWQYGLQKMYLLMEKQHNRGQEG
                     AGLATVKLDMPPGEEYIWREREEGNNAIQQIFANINEKLDQNKSVVEDAVFAKNKLPF
                     AAELYLGHLRYSTTGRSGIANVHPFLRRHNWKARTMVLAGNFNLTNVDELFEMLTNQG
                     QHPRDKADTFLMLESLGNKLDNEVQAEYEKIRQRYSNDIIITQKIEEQLDIPSFIRKS
                     EKNWDGGYVIAGMLGHGDAFVFRDPKGIRTGFYYKDDEVVVVASERAVIQTVMGASDE
                     QIFELEPGKSMIIKKNGNILFDTIRMAAPELKPCSFERIYFSRGSDLAIYKERKKLGA
                     SLAGSIMHAIGNDMDNSVFSFIPNTAEVAFYGMIQGIRDMSGIDPHVEKVLIKDIKLR
                     TFISQNKQRNDLAAHVYDVTWNSIRREKKDNLVVIDDSIVRGTTLKQSILKILSRLEP
                     KKIIIVSSSPQIRYPDYYGIDMAKMSEFCAFRAAVALLKETGRQSVIDEVYRKSKAQE
                     NLPKEMIVNYVTDIYKSFTQEEISEKIAAMLTPDEVDCPVQLVFQTIEGLHAACPDHT
                     GDWYFTGNYPTPGGNRLVNKAFINYYEGK"
     gene            complement(12181..14022)
                     /gene="glmS"
                     /locus_tag="PKH68_04615"
     CDS             complement(12181..14022)
                     /gene="glmS"
                     /locus_tag="PKH68_04615"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNX12254.1"
                     /translation="MCGIVGYMGNKQAYPILIKGLHRLEYRGYDSAGIALIDNDKLNV
                     YKSKGKVADLEHFAEQKNITGTIGIAHTRWATHGEPNQKNAHPHYSQSEELAIIHNGI
                     IENYAVLKQGLAEHGFIFKSQTDTEVLVQLIEYMKKTNRVDLPTAVQLALNQVIGAYA
                     IAVIEKSNPNLIVAARKGSPLVVGIGDDEYFVASDATPIIEYTDKVVYIGDEEIVTLR
                     RNEPMKIMTIGNIEKTPEIHKLEMTLSQLEKGGYPHFMLKEIFEQPVTLADSMRGRVN
                     VDANNITLAGFIDNKEKFMNAKRIIITACGTSWHSAQIGMYAIEEFARIPVEVEYSSE
                     FRYRKPVINKDDIVIAISQSGETADTLAAVELAKQNGAFIFGICNVVGSSIPRNTHSG
                     CYTHVGPEIGVASTKAFTAQVTVLTMLALLLGKEKGTLSEENYLRLINELNLIPNKIS
                     QILKKNQTIAEFAKTFTYASNFIYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMK
                     HGPIALISQEMPVVVIATNSSTYEKVISNIQEIKARKGKIISIITEGDEIVKNLSDYS
                     ISIPEADECLSPLLSVIPLQLLAYHIAVVKGCDVDQPRNLAKSVTVE"
BASE COUNT         3843 a         2922 c         2557 g         4659 t
ORIGIN      
        1 ccaacttgtt tttatcacgc agtccgccgc atttacccaa aaaaagcact gcacgcggat
       61 gaatagccga taatatatcc atgatcatag ctgcgtttgg acttcccatc ccgaaattta
      121 tgatcgtaat tccatctgcc gatgcattta tcatgtttcc atctctgccc agtaccggca
      181 cattgaacca actggcaaac atttccacat aattggaaaa atttgtaagc aaaatgtatt
      241 ctgtaaactc ttccagcggt cttttggtat atctgggaag ccagttttga actatctctt
      301 cttttgtttt catataatct tactttgtag tactgaatta gcaactaaat atttttgtat
      361 cttcgtactc agttttatta tgattgacaa aaatacaaaa aaatgtggca actcaactta
      421 ccagaatatc aatttagatt aaaacagact gaaaaaggct atttcgtact ggataatttc
      481 cggaaaagat ttgtgaaact aactcccgaa gaatgggttc gccagaattt tttacgcttt
      541 ctgactgata ataaacactt tccatcggcg ctgattgctg tagaaaaaca actgacaatg
      601 aatggaatga aaaaacggtg tgatgctatt ctctacgata tacaggccaa cccggtgatg
      661 attattgaat ttaaggcgcc gaatattaca atcaatcagc aggttttcga tcaggtagcc
      721 gtttataatg ccaaattgaa agtgaattac ttcctgatca gcaacggaat ggagcatttt
      781 tgctgcaaag taaatgtcga aaatgcctcc tactcttttt ttgaaggaat tcccgaatat
      841 gaggattttt gctcactgac tacctgacga tggaaaagtt ataaattccc cctttagggg
      901 gttagggggc ttttttcttt aattctctca aagcatcctt agcaatccac ctggctgatt
      961 ttgtattttg tgccaatata cgtttagctg tttcgatggc ttttcttcgt aagttttcat
     1021 ttcgcttacc tatctgccgc aaggcccagt ttacggcttt gcgaacaaaa ttacgttcat
     1081 cccatgcttc cctttctata atttggagca ttggataaaa gaaatcatcc ggttttttct
     1141 tgtcatgcac tgcaaactga cacatgagaa caaaagcggt tcgtttcacg aattcctcac
     1201 tcctgatggc atattcatca attttatttc ttgcgaatgc agcaccttgc aacattttac
     1261 acgcacagtc gcacatatcc cacgaatcaa aatccttaac caggctatcg tattggctgt
     1321 tggtgagtaa ttgtgcatcc attatcattg tggccagcaa tttggcttcg tgaatgtcgg
     1381 tctgccacaa ttcaaatccc aaaacctgat tttttccaat ccttttagcc agtttgcgca
     1441 ggtcgggcaa cttaattccc aaggccttat cacctggaat tccaaaatgt gataacttgc
     1501 tcaaatgatc agccgtggct aattttttca attcgactat aacttcgtca acttccattg
     1561 gtaatcttat taaaaaaaac acttaccgga ctgctttcaa gtctgataag tgttttttct
     1621 gttcagtatt tttcacatta cgagtggcct cccacgacta ttcgcatgat tttcagattg
     1681 agaacaaaga agcgaataag ttgaaacaga atacttgtac gccagaattt tgtccatttt
     1741 gttggttccg tggcaaatga tgcttcgctg tacggaattg ttttttccag gtctgccata
     1801 tttctttatt tttataatat tcatttttta catctcatta tttattcagg tatcatccac
     1861 cacagtggca gtgctttagc tttatagata aacatatagt gcataaaaag cttcatccag
     1921 tgtcctgcca ggcctatttc tcctacagtg taacctatat ttctccccca tttcggaaat
     1981 ttttcccaat cctgcacaat gggaaatact gtcatcactg cggctttgcc atgtctgata
     2041 ccatagcctg cagatacgat acaggcagca cccattttgg ccattgatgc ctcatgtttt
     2101 agttctttat ttcctgattt tatctgttca gtaatgttga gagcaacaat acgccccata
     2161 acgccggaag gcataccggt acgaggagcc gtagccgtaa ttgatgtccc gtttttggaa
     2221 gtcatgggtt ttgaaatagt atgtggagga gcaaaagcga tacctgcagc aaatatatta
     2281 tcgtacaaag ggctgttgta cgtttttggc caatcacttg ccttccattc atcaaatggt
     2341 ttgggtgtat aatcggcatc tactttcatc aaaccattgg gagcaaacat ctgagctgta
     2401 atgtcttcca tctttttatt gaaagcttta agcccaacac cagcaaaggc aggtattagc
     2461 attgcgaagt caaattccag tttatgatat tcacctgcca gattttcata actgaccaca
     2521 cctggttcaa ctttttgtac agccgaacct acgatccatt ttatgccata ctctttgaaa
     2581 atagattcgg caaaaatttt ggtaggtgta acgtatccac cctgttttac ataggcacct
     2641 cccataccaa agtcacccaa ttcatattca tttgatatcc aaatgatttc agctaaatcc
     2701 agcagtttac gccgctttat ttcaaaggca atgttcagcg catactcaaa tgccgccccc
     2761 tgacaggtag ccattccatt tccggtacca atcagaaaac gttgtttttc acctttttcc
     2821 atccttgcaa aacatttctg gagattttcc caggtatggg cggcatgatc gtaggtacaa
     2881 actgaaaaac tgtttaaatc gggacctaaa ccttcggttg ctgcaaaatt tagtttcgga
     2941 ccggtggcat tgatcagata atcatactcc actttctcag tctgttcttt attatctcct
     3001 gcaacatact gaataacaac gtattgtttt acgttcgtat cgtcaccttc gggatgaatg
     3061 gacagggctt ttgcctgttt gaatataata ccttgttttt tataaaccgg agctaacggg
     3121 aagatgactt gcttggtagt catgagaccg atcccaaccc aaatatttga agggacccat
     3181 tgataattgc tgtttgggct caccaccact acttcgtgtg attttcccaa cttacgcctg
     3241 gcaattaatg ctgccgtgtg accggatata ccggcaccca aaattagtac tcttgccata
     3301 aatgattaat tttgatgaaa ctctattaat atttttttaa atgaatttcg attgacatgc
     3361 atacccgttg ctaaagtcct tattgacagg tatttttttc atttaagata tggcaaatat
     3421 ataaataatt ctgaatagtg atactctaat agaataaaat aattaaaaaa tatttttttc
     3481 tacaaaaaat atcccggaaa tcggaggttg gatattattt cttttgtgaa ggttgggaat
     3541 tttacgatat tacaacacca gatatcaaat ttcaagggca ccaataatat ctttggctga
     3601 tgaaaagcca tttttattta aataattatc gataccttcg actattttta cggaaatcgc
     3661 cggatcaata aaatttgccg ttcctacctg aatggcagta gctcctgcca gcataaattc
     3721 aatagcatca gtagcgttca ttataccacc cagaccaatt accggtactt tcactgcttt
     3781 agccacctgc cagaccatac gcagagcaat cggtttaaca gccggaccgg ataaaccccc
     3841 ggtaatggtc gaaagtactg gtttgcgggt ttttgcatca atggccattc ccaacagggt
     3901 attaatcagc gaaatgctgt cggcaccggc accttccacg ccttttgcaa tctccgcaat
     3961 atcggttacg ttgggcgaaa gtttgaccat caactctttg tgatatactt ttctgacttc
     4021 tgccactacc tgtgaagcag ccagacacga aactccaaaa gccatgccgc cttctttcac
     4081 attgggacag gaaatattca gttcaatacc cggtattttg tccagtgtat tgataatttc
     4141 agcagttttt atgtagtcct caatagtaga accggacaaa ttaatcatta catttgtatc
     4201 aatgtctttg agggtcggat aaatatgatt tacaaagtaa tcaataccct tattctgcag
     4261 tcccacagcg tttaacatgc ccgagggggt ttctgccatg cgtggatatt ggttgccctg
     4321 acgttcgcga agagtggttc cctttacaat aatgccacct attcgcgaca agtcgatgaa
     4381 gtcggaatac tcagtaccat agccgaatgt acccgatgct gtcatcaccg gatttttcat
     4441 ttgtaatttt cccagttggg ttgttaaatc tgccatttca actttgtaat gttaaagatt
     4501 ggaccttcgg tgcacacaca taaattaccc tctgtagtat cagttacgca gcacaggcaa
     4561 gctccgatac cgcatcccat catattttcc aatgacactt cgcagaaaac gccattaatt
     4621 gctgcgtaag aggctacggc tttcatcatg ggtgttggcc cgcaagtgta aatataatcg
     4681 aaatttgagc caattaagag cgaatgatgg gtaacgtagc ccttttctcc atacgatccg
     4741 tcctcggtgg tggtgtacac cttgccatac cgttcaaatt cgtcaagttg aagcaaatcg
     4801 gttttggaac gggctccaag tagaaaatta cagttatatc ccatactgtg gagtttttca
     4861 ccgaacatta aaaggggtgc aattcccact ccgccaccaa tcaatactat gcgggcattt
     4921 ttttcggccg gcatagtgaa tgtatttccg agaggcagga tggcatttag cgtttgccca
     4981 acttccatat cggccattcg tctggttccg tcacctacta tctgaatgag cagccacagt
     5041 tcattttttt cgtaatctac aaaattaatg gatatgggac gccttaaaaa agtggtgggt
     5101 gaaccatcaa cgcggatttc ggcaaattgt cccggcagaa tttcaggaag ttgatcaata
     5161 gttgtcaatt tcagaagtac attctgttca ttgagttgta ctttatgcgt aatggtaagg
     5221 tcaagaatga atttcttcat ctgatttcgg ttattaattc tttcaccgta gttggttttg
     5281 caaaattacg aaatttcgaa cgaatttcaa actctgtcga ccatttcctg aatttcggat
     5341 tttagtaaaa ttttaatttc cccttcggct attttgcttc gaattcctga aaggtattat
     5401 catcgtaata aacaatgatt tttttgataa actttgattt gtcggtagtg acaggaggga
     5461 tttttattgt ttgcgatgaa tctgcaaccg gttttatata actgatagat tcttcggtta
     5521 catttttttc aacaatattt ctaccgtaac cagctgattt tgtgtctatt tcatccatat
     5581 tttcgaacaa agtgggcgtc tgagatttct ttgaatcccg atacatttgc ccgatgccca
     5641 aaataagcca gtctgaattg attgtacggt aacggtccag gatttttttc aatacatcaa
     5701 gacttggatt gtttcttcca ctaagtatgt gtgatagtgt aggagtttta atgcctatct
     5761 cagatgcaaa ctgtgttgcg tttaagcctt cggcctgcat gaccttttcg attcgttctt
     5821 tttcagtggt ttccatttgt agtaaagtca aaagtttaca aataaaattt tccacaaata
     5881 tatgtaaaca aattcacatt tgcaaatagt tcacaaatgt aatttactgc tttactaaaa
     5941 tacatttgta aacttcattg aattatttga atttaaccta taattagacg ttatctattg
     6001 taatacatat cattaatgaa ataatatcaa caattaataa aaataaattc tttgtttata
     6061 taatatatct tataaaattc attatcagca caataaatac aataataact gttatttgtg
     6121 gctgtgaacc cattttatca caatcgtaaa cacacattta aagtattttt ttatatattt
     6181 cttttattaa attggtttat agcattttac atacattttt catttcatat taattctaat
     6241 taattttaat tgtgacaaat gtttatctat ttacgtatgt atattttaat ttttacaaaa
     6301 ccaactatac cgctaatgaa atacacaaaa acatttgtaa attgacacat gtgtaattgt
     6361 atattttaca tttgtaattt acgtattaac tcctataaga ataaataata aattgattgg
     6421 tagagtaaga aaagaatttg tgtggtgaga aatatccgga gcatcaagta acaacttgaa
     6481 ttattcttaa ggcagccacc taaatgaaaa attttggtaa taccctacct ggtaaaaaaa
     6541 tcagtatgac gatgtgaaga aataaatcct aatcaaaact aaccggaaat actttacgta
     6601 aaatcgtcga tgaggaggaa gtcgaaatta aatagtggat aaaagaacag gctattcgaa
     6661 attattggtt gatagggaaa aatatccgat ttataacccc taaattgatg tttaaactaa
     6721 tttatctgtt tttccaacaa agaaaaacag ctctatctaa ctgattgact gcgatttatg
     6781 caaataaaca gccgctgaga atcgaataaa aatatttttt ttatacctgg ggttaaatga
     6841 cagtaatttt gtaatgtttt cgcataaaaa aaccggacaa attgctttgt ccggattttc
     6901 ttatgttgaa attatttaaa cttattttac ttcaggattt tgttctcccc ctatttcagg
     6961 atccaccaga atacgaccgc agtattcgca tacgattatt ttttttcgcg aacgaatatc
     7021 caactgccgt tgtgccggaa ttttattaaa gcagccacca caagcatcgc gttgcactga
     7081 aaccacagct aatccgttgc gggcattttt gcgaatacgt ttgaaggcgc tcaccaatcg
     7141 ggcttcgatc tttgcctcaa tcttcttagc atgttcacgc aatttttctt cttcctgttt
     7201 ggtttccgat acaatctcgt ccaattcccc ttttttctgt tcaagatcaa gtttacgctc
     7261 cgataactga ttgaatgtgc tatctttttc aattttctta ttctcaagat tggcagtaaa
     7321 ctcccgaatc cgtttctcgc taagttctat ctccagtgtt tgaaattcta tttctttcga
     7381 cagattatca tattcacggt tatttcgtac attgttctgt tgctctttgt agcgttcaat
     7441 tttaatttta gcttcttcga tctctacctt ttttccttta atggcagtgg ttaattcggc
     7501 tatttcattt tcaaaatttt ccaaacgtgt acttaaacca acgatttcat cttccatatc
     7561 ctgaacttca aggggaaggt cgccgcgtaa tatcttgatt tgatcaattt ctgaatcaac
     7621 cttttgaagc tcgtagagag cttttagttt ttcttcaacg gtgatttctt tatccgtttt
     7681 ggtcacagta gccatatatt ataaatattt tattggattt gtatttacat ctgaaattcg
     7741 gactgcaaat ttaggaattt tttttgtaat tatctcataa aaaacatctt ttgtgaactg
     7801 ttcgctttca taatgtccga tatcaacaat taaaatctta ttttttgcat caaaaaactc
     7861 gtggtatttg aaatcagcgg aaataaaaac atcggcacct acagctattg cctggcgtgt
     7921 taagtcgctg ccggagcctc cacatacagc tattttccgt attttacggt tcaaaaggga
     7981 ggtatgccgg agtgtttgga ggtgaaatac cgatttgagt tggtggagga attccatttc
     8041 atcagtttca gcgggtaact cacccacgat acctgcacct accgaattcc acgtattctg
     8101 gagggctata aagtcgtaag ctggttcttc gtagggatgc gaacgaagta aagcgttggt
     8161 tatctttact ttcagatatt cggggaaaat cacttccaga cggatttcgg attccgtatg
     8221 ttcaatttgg atttcgccaa caaatggatt tgccttctcg ttggccctaa aagtcccccg
     8281 tccatcagac atataactgc aattgtcgta attgccgata ctgccggcac cggcttcaaa
     8341 taaggcattg cgaacctgtt gtatgaaatc tgccggaaca aatgtaacga gtttaaggag
     8401 aagttctttt ttgggttgaa gaatttgccg gtttttcaag ccgattttat cggcaatttt
     8461 gccatttaca ccttccatca cattatccat attggtatgt gcggcatata cagcaatttc
     8521 attttttata gcttttagga cacaacgctg tacgtcattc tgtccggtaa tattttttat
     8581 tcctctgaaa atgaggggat gatgtgaaat aatcagatta caaccatttt gaattgcctc
     8641 atcgacaata tcttcggtta catccaccgt aagaagcaca ccggtaacaa gcatatcggg
     8701 acttcctatc agaagtcccg cattgtcata ttcttcctga agagcaagag gcgcagcctc
     8761 ttcaatccat tgtgttattg ttcttacagt tgtcatctat ggtactaccc cattttacgg
     8821 ctgttagttg atcgttgcga agccgggttg cttactttgg tctgttgttt tggctgtttt
     8881 atgtcttttg gtacatttac cgctttagct tcttttttta gtttatcgtg caaatcggtt
     8941 tctttgtcag cttttgtatc cacttcagct tcttctttag ccaaaaaatc aaccataccc
     9001 acctgggtca ggagattgta ccagctgatt aacttgcgaa tatcgctcgg ataaacccgg
     9061 tcacggtcgt attcaggtaa cacttcaccc atgtattttt tcagttgatc attgtcggct
     9121 ttggggtcga tggaacattt tgcaccattt tctttggctt tgatattttc caatacctga
     9181 cctagtggca tttcttcagt atcggtaaaa atagcgatat cgcccagtga aacggctttg
     9241 tctttcgtat atgcagggat gcgcttgcca tcgattaacg attctacgat gagcatgttt
     9301 tttccgttcg ataccaattt gtacaaacct ggttttcctg tgatagataa aatttctttc
     9361 agcataataa ttttcaataa atcagacttt aaacgtccgg taaataatat ttttttaata
     9421 attcaattgt gtcagagaca aaaaatgatc cgataattta ctcaccagat caaatccagt
     9481 acaaatgtag ttatttattt tacattttgt attcaccaat actctaaaaa aacacggata
     9541 actcattcaa tttgactttt ccttcgtaaa ttgcttttcc aaggataaca gccggcacgt
     9601 ttgcattgtt cagtttttcg atatcagcca tcgaacttac acctccactt gcaatgagat
     9661 atatttgtgg aaattcaccc aagattttct catacaaatc gaatgcaggc ccttcaagca
     9721 ttccgtcttt actgatatcg gtgcaaatta ttttggtgat gcccttgtcg atgtaatttt
     9781 tcagaaaagg tatcaattcc ctgtcagttg tttcggtcca gcccgttaca gccacttttt
     9841 catccatcac atccgctccc agaataattt tttcggagcc aaaggaattt atccagttat
     9901 tgaaaatatc cggatttttt actgcaatgc tgcctcctgt tatcattgag gcgccgcatt
     9961 cgaaggcaat ttgcaaatct ttgtccgatt tcagtcctcc tccaaaatcc acaatcaggt
    10021 tggtaaaaga acaaatccgc tcgagaactt tatgatttac aatgtgttgg gctttagcac
    10081 catccagatc taccagatgt agccgggtga ttcctgcacc ggcaaacatt ttggctactt
    10141 ccagcggatc ttcgttgtat atttttttcc gctcgtagtc gccacgtgta agtcggacac
    10201 atttaccatc aataaggtct atagcgggga taatttcaat cannnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nntcttttcc ttcgtaatag tttataaaag ctttgtttac
    10381 caaccgatta cctccgggtg tgggataatt gcctgtaaaa taccagtcac cggtatggtc
    10441 tggacaggca gcatgcaatc cttcaatggt ttgaaaaacc aactgtaccg ggcaatcaac
    10501 ttcatcggga gttagcattg cggcaatttt ttcggatatt tcttcctgtg tgaaggattt
    10561 gtaaatgtcg gttacataat ttacaatcat ttctttgggg agattttcct gagctttgga
    10621 cttacggtac acctcatcaa tgacactttg tcggccggtt tctttcagta aggctacagc
    10681 ggctctgaaa gcacaaaatt cactcatttt ggccatatca atgccgtaat aatcgggata
    10741 tcgaatttgt ggtgaggatg aaacgatgat tatttttttt ggttccaaac ggcttaaaat
    10801 tttcaatata ctctgtttca atgtagtccc gcgcacaata gaatcatcaa ttaccaccag
    10861 attatctttt ttttctctgc ggattgaatt ccaggtcaca tcatatacgt gtgcagccag
    10921 atcatttcgt tgtttatttt gagaaataaa agtcctgagt tttatatctt ttatcaacac
    10981 tttctcgaca tgcggatcga tacccgacat atcgcggata ccctgtatca taccatagaa
    11041 agctacttca gctgtattgg gaataaaaga gaacaccgaa ttgtccatat catttccgat
    11101 ggcgtgcatg attgatccgg caagtgaagc cccgagtttt ttgcgttcct tgtagatagc
    11161 cagatcgctg ccccgtgaaa aataaattcg ttcgaacgaa catggtttca attcgggagc
    11221 tgccatgcga atggtatcaa acaggatatt tccattcttt ttgataatca tcgatttacc
    11281 cggttctagt tcgaatatct gttcatcgct ggctcccatt accgtctgaa ttacagctct
    11341 ttcggaggca accaccacta cttcatcatc cttatagtaa aaaccggttc ggataccttt
    11401 cggatcgcga aaaacaaaag catcgccatg gccaagcatt ccggcaatga catatccccc
    11461 atcccaattc ttttccgatt tgcggataaa agaaggtata tccagctgtt cttcaatttt
    11521 ttgggtaatg ataatatcat tcgaataccg ctgtctgatt ttttcgtatt ctgcctgtac
    11581 ttcgttgtcc agcttatttc caagcgattc gagcattagg aaagtatcgg ctttatcgcg
    11641 tggatgctga ccctgattgg tgagcatttc gaacagttca tccacattgg taagattaaa
    11701 gttgcctgca agtaccatcg tcctggcctt ccagttgtgc cgacgcagga atggatgaac
    11761 gtttgctatt ccacttcgcc ctgtggtgct gtatcgtaaa tgtccaaggt aaagttcagc
    11821 tgcgaatggc aatttgtttt tagcaaaaac agcgtcttcg acgacgcttt tgttctgatc
    11881 cagtttttca ttaatattgg cgaatatttg ttgtatggca ttatttccct cttcacgttc
    11941 acgccatata tattcttcgc ccggcggcat atccagtttt acggttgcca gtccggcacc
    12001 ctcctgtccg cggttatgtt gtttttccat caacaggtac attttctgta acccatactg
    12061 ccaggttccg tatttctcct gataatattc caggggcttt agcagcctga ccattgcaat
    12121 tccacacatg attttttctt tttttagacc ttactttaaa taataaaata cagaaataat
    12181 ttactctacg gtaactgatt ttgccaggtt gcggggttga tccacatcgc agcctttgac
    12241 cacggcaatg tggtatgcaa gcaactgcag gggaataact gagagcaacg gtgacaagca
    12301 ttcatctgct tcggggattg aaattgaata atcggacaga tttttaacta tttcatcacc
    12361 ttcggtaatt attgaaatta ttttaccttt tcgtgctttt atttcctgta tattgcttat
    12421 tactttttca taagtcgaag aattggttgc aattaccacc accggcattt cctgacttat
    12481 tagtgctatt ggtccgtgtt tcatctcggc agcgggatag ccttcggcat gaatatagga
    12541 tatttctttc aatttcaatg cgccttccaa tgcaaccgga aagttataac cacgtcccag
    12601 gtagataaaa ttactggcgt aggtaaacgt tttggcaaat tcggctattg tttggttttt
    12661 ctttagaatt tgagaaattt tattgggaat taagttcagt tcgtttatca gccgaaggta
    12721 attttcttca gataatgttc ctttttcttt tcctaaaagc aaagccagca tggtcaggac
    12781 agtaacctga gctgtgaaag ctttagtgga tgccactcct atttcgggac ctacatgcgt
    12841 ataacaacct gaatgtgtgt tacgcggaat ggatgaacca accacattgc aaattccaaa
    12901 aataaatgct ccattttgtt ttgccagttc tacggcagcc agggtatcgg cggtttcacc
    12961 cgattgagaa atggcaataa cgatgtcatc tttattgata accggcttac ggtagcgaaa
    13021 ctcggaggaa tactccacct ctacaggtat gcgggcaaac tcctcgatgg cgtacatacc
    13081 tatctgagcc gaatgccacg acgtgccaca ggcagtgata atgatacgtt tggcattcat
    13141 gaatttttcc ttattatcaa taaagccggc aagcgtaatg ttatttgcat ccacatttac
    13201 cctaccccgc atactgtcgg ccagcgtcac gggttgttcg aaaatttcct tcaacatgaa
    13261 atgtggataa ccaccttttt ccaactgact cagggtcatt tccagtttat gaatttcagg
    13321 tgttttttca atattgccaa tagtcatgat tttcatcggc tcattgcggc gcagggttac
    13381 gatttcttca tcgccgatat aaaccacttt atccgtgtat tcaatgattg gagtggcatc
    13441 tgaagccaca aaatactcat cgtcgccaat accgaccacc aggggactac cttttcgggc
    13501 agctacgatc aaattcggat ttgatttttc gattaccgca atagcatacg caccaataac
    13561 ctgattgaga gcgagttgta ctgcggtagg gagatcgact ctgttagtct ttttcatgta
    13621 ttcaataagc tgtaccaaaa cttccgtatc ggtttgactc ttaaaaataa acccgtgttc
    13681 ggcaagtccc tgtttgagca ccgcataatt ttcgataatg ccattgtgaa tgattgccag
    13741 ttcttcggac tgcgaataat gagggtgtgc atttttttga ttgggttcgc cgtgggtagc
    13801 ccagcgggta tgggcaattc cgatggttcc tgtaatattt ttttgttcgg caaaatgttc
    13861 cagatcagct actttgcctt tagacttgta aacattcaat ttgtcattat caataagtgc
    13921 aattccggca ctgtcgtatc cgcgatattc gagccggtga agtcccttaa tcaatatcgg
    13981 gtaggcttgc ttgttgccca tatagccaac tattccacac atattattta tttttttagt
    14041 tttttaatct gtttaatgcc ttaagtgtat cttcacgact a
//
LOCUS       DAOPZK010000050        13658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_4938_length_13658_cov_21.234654, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000050 DAOPZK010000000
VERSION     DAOPZK010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13658
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4938_length_13658_cov_21.234654"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..957
                     /locus_tag="PKH68_04620"
     CDS             1..957
                     /locus_tag="PKH68_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HNX12255.1"
                     /translation="MEANNHHAISYGDDTITEETNRLFEKIFGEVKVLYAFNGTGANV
                     VALKCCTLPFQSVICAETAHIQVDECGAPTQSIGCSLVALPTPDGKLTPELIRPELKH
                     RGNLHHTQPKVISISQTTELGTIYSPDELKALSNFAHENDLYLHVDGARISNAVAALG
                     CSLKEATVDCGIDIMSFGGTKNGLMIGEAILIFNKELWKNAPYLHKQSAQLFSKNRFI
                     AAQFKALLENELWRTMALHSNKMAQLLKNEIIKIKGVILTQDVQANSLFVIIPAHAVE
                     PLREAYRFYDWNTATGELRWMCSFDTTEEDVLNFVTTLKSLL"
     gene            1014..2033
                     /locus_tag="PKH68_04625"
     CDS             1014..2033
                     /locus_tag="PKH68_04625"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNX12256.1"
                     /translation="MITKELEKYISTIPKTELHLHIEGSFEPELMFEIAQRNQVNIKF
                     KTVEELRQAYEFGNLQDFLDIYYDGANVLLHEQDFYDLTMAYFRKCKQDNVVHTEIFA
                     DPQTHLSRGVSMKTIIDGITRARRDAEREFGISSYLIVCFLRHLDENSAIEMFEEALQ
                     FRDKIIGVGLDSSEVGNHPEKFKRVFARAREENFRLVAHAGEEGPSDYIWGALRELNV
                     SRIDHGIRCMGDEELVEYLAENQIPLTVCPLSNLKLQVVKSLTHHPLKEMLDNGLLAM
                     VNSDDPAYFGGYVNENMLQTADALNLSENDILQLAKNSFIASFLPDEEKEKWLTELDN
                     FRISN"
     gene            complement(2109..3062)
                     /locus_tag="PKH68_04630"
     CDS             complement(2109..3062)
                     /locus_tag="PKH68_04630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNX12257.1"
                     /translation="MFSTQIDIPHSAVEITYNDRILTLGSCFAENIGTKLQEAFFLTF
                     INPFGVMYNPMSVAQGIRHLLSEKEFTAADLFQSGSLWNSLAHSSTFSAVTADEALQK
                     INSRLQAARYFLHETNVMMITLGTAWVFELVDTRKIVTNCHKLPASRFTRRRLSVDEI
                     INEFTEVFGLLRNSYPGLKFIFTVSPIRHWKDGAHENTVSKSTLHLAVDALEKQFDFV
                     HYFPAYEIMMDELRDYRFYASDMLHPSDVAVDYIWQRFGDTYFSSETLNLKKELERLR
                     ADLNHRPLHPETEEYRLFQLSVEKKKNGLIQDYPFLKERLQ"
     assembly_gap    3278..3377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3378..3847)
                     /locus_tag="PKH68_04635"
     CDS             complement(<3378..3847)
                     /locus_tag="PKH68_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12258.1"
                     /translation="MKTKNVMMTLVAVFISATMISAQTPVVTNAAETQKKECADKAKH
                     ECKNANAEVKHECTQAKTECTKADSMKKHECAQGQAMAKHECKEGKAMAGADKAGCCK
                     DGKAMAGADKAACKDGKAMAGADKAGCCKDGKAMAGADKAACCKDGKAMAGADKA"
     gene            complement(3948..4274)
                     /locus_tag="PKH68_04640"
     CDS             complement(3948..4274)
                     /locus_tag="PKH68_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNX12259.1"
                     /translation="MKTKYVLTTILMVFALSINLQAQTKKDQSKKTVMFNVSMHCESC
                     QRKIEKNIAYEKGVKDMAVKLEDKTVTIKFDTLKTNESKLIKAFNDLGYEANVVKTET
                     PVGNRK"
     gene            complement(4302..6530)
                     /locus_tag="PKH68_04645"
     CDS             complement(4302..6530)
                     /locus_tag="PKH68_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX12260.1"
                     /translation="MKKQIILILCISITTQLLSQVHGYVKDKLNEAIPGVYVRWAGNN
                     DGVVTNADGHFQLNPEQGNNLLVFSNVAYNSDTVDVSKSKQTNQLEITLDDAIQLKEV
                     TVTQQRLGKIKSRTAVFNVETLNTHELRKAACCNLSESFETNASVDVSYSDAATGAKQ
                     IKLLGLSGTYVQMLTENIPNLRGLSSAYGMGYIPGPWMESIQISKGTASVKNGYEAIA
                     GQINVEYKKPQTSDKVSLNLFTNSTGRVETNADVAVKLSEKTATSVLMHASDEFMTLD
                     DNRDGYMDLPKVKQYNLANRWFYNSGGYILQAFVRGLSEKRTGGRTDDSYKIGVNTER
                     YEFFVKNGFLFGDNDQHHNHSENEPVEKHNQSSLGIIFNGTIHNQDARYGLKQYDGHQ
                     SNLYANLIYDASIGTQHKISTGASFNMDVINENLNLNSLPGEFNRTEYIPGIFGEYTF
                     TKDALTAMAGLRVDNNSVYGTFITPRFHVRYNILDHVHLRASAGKGYRSPNVLAENNY
                     LLASNRSLHIAGDLKMENAWNYGFTVHGFFPLFGKELSLMGEWFYTDFNNQVVADMDT
                     NPHAVSFSNLRGKSYSSSFQIEANIEPVRGLTATFAHRINDVKTTINGNLREKPLTNR
                     SKSLASLSYQTPLRKWQFDFTTQFNGGGRLPDPDPAHPLWEREFKPFTIMNAQVTKNF
                     KTWSVYGGAENITGFVQHNPIIDVQNPFGTDFDASMVWGPMHGRTLYLGFRWALTGKD
                     KL"
     gene            complement(6626..7087)
                     /locus_tag="PKH68_04650"
     CDS             complement(6626..7087)
                     /locus_tag="PKH68_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12261.1"
                     /translation="MKTPLTHIIISILLSCIFLFAGSGFNLISYCCNTCAEAGIEKVA
                     EMSCECIHHHHDADGSEHHQHTTVENICGHDLEKGCDLTRLTVDVPSMQLNEDFLMDY
                     SLLYADLSSLWTDFITGFETADIKTTVEHNSQLFIPLTGRRILSENSILLI"
     gene            7301..8116
                     /locus_tag="PKH68_04655"
     CDS             7301..8116
                     /locus_tag="PKH68_04655"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNX12262.1"
                     /translation="MNKYLLFSASLLLILVNSCTKQEMEMKNYSYANTFHLTPDTTLG
                     GLSISIEAELPVNFSNKEILKSIQQQIVGKMFGEIYTNLPVDSIIPSYVSRLYNDYKI
                     SYGTDLEKYKNLKGPMLSNEIQVEAVSMFLDDKILSYSYERYAYMGGAHGNSNRYFYN
                     FDLRNGHILHESDIFIDNYKAPLTELIKQRIVEDNAEIESVADLTEFDFWESEIKPNG
                     NFYISDEGIVYVFNPYEIAPYSTGQTEVTLPYDRLKVLLKPGNLISYLYSTPA"
     gene            8134..8670
                     /locus_tag="PKH68_04660"
     CDS             8134..8670
                     /locus_tag="PKH68_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX12263.1"
                     /translation="MSFLELLKNRRSIRRYTDRKVEPEKIQQILTAALMSPSSKRSNP
                     WEFIVIEEDEMLQKLSACRPTGSQLLAGSPLGIVVIADQTRTDVWMEDASIAAIIMQL
                     EAHDLGLGSCWVQVWGRQKEEGVSAEAYIKELLSIPDEFGVLCIISIGYRDEERKPYD
                     TEKLDYNKIHFGKFNKQP"
     gene            8695..9012
                     /locus_tag="PKH68_04665"
     CDS             8695..9012
                     /locus_tag="PKH68_04665"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNX12264.1"
                     /translation="MKDNRLAWGISLLLFGVMFLLRQLGIFSPEIADVLFDFKNFPLI
                     IGVIFLIWHKNKSIGMILIVVGLLLRLQEIIHWTQNLSEFVWPLLLIAAGIILLFGVK
                     KGK"
     gene            9049..9816
                     /locus_tag="PKH68_04670"
     CDS             9049..9816
                     /locus_tag="PKH68_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNX12265.1"
                     /translation="MKIVLLGAGNVATHLGLRLIQTGVELLQVYSRTASSATELGKLL
                     GVPHCSDLKQLDKTADIYIYSIKDSALSEVISGVNIPDAIHLHTAGSIPMDIFGGFAH
                     HYGVLYPLQTFSKAKPLDFSQIPLFLEANDDHTAGILTQLATRLSSVQYYLSSEKRKK
                     IHLSAVFACNFTNYMYRIADDIVSETGLPFDVLQPLITETADKIRYLTPTEAQTGPAV
                     RNDEPTMLTHINLLKENENALKTYITLSEQIKELYKK"
     gene            9817..10803
                     /locus_tag="PKH68_04675"
     CDS             9817..10803
                     /locus_tag="PKH68_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNX12266.1"
                     /translation="MEAYLRIVRWKNLLFILVIQWLMQHAVVYPLLQTYGFEISQRDV
                     FNILLYAATMLISAGGYVINDYFDVKIDRLNRPDKVIVSEKITPKAAMLYYQMLTVTG
                     VICGLILAFLSHSFTLGFIMLFTPGLLWFYSASYKRQFITGNVIVSFSASLVMLVVGF
                     LEVALLQIQYGNLLLQTPIPAQIYGWTGGFAAFAFACTWIREIIKDMEDIHGDREMEC
                     RTMPIKWGIPKTKYFLYALIILTIAALFHVDFHYIHFDGNLTTRYILFGLTLPLVALS
                     YLIFRSKTPEDFHQASTLSKFIMLAGVLYSLIFYYLTAKTFHISIFGLFIVK"
     gene            10809..11387
                     /locus_tag="PKH68_04680"
                     /pseudo
     CDS             10809..11387
                     /locus_tag="PKH68_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
     gene            11392..12111
                     /locus_tag="PKH68_04685"
     CDS             11392..12111
                     /locus_tag="PKH68_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNX12267.1"
                     /translation="MNKVTVCISPALYSFYAQKEAIVIIVDIFRATTSICAAFNNGAR
                     SIHTVATVEEAIHYKEKGYLVAAERNVKKCNFADFGNSPFDFSHEKVADKEIIFTTTN
                     GTQAVQMALDADEILIGAFSNITTLTNYCNSKKKNVLILCSGWNNRFCLEDTLFAGAL
                     TEKLIKTGKFCSDSDATLAALELWKIAHPDLINYICSTEHYQRLKNNGLEDSVEYCLT
                     NDTTPVLPVYDKINRIFILKK"
     gene            12119..13525
                     /locus_tag="PKH68_04690"
     CDS             12119..13525
                     /locus_tag="PKH68_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HNX12268.1"
                     /translation="MKLQHSIQRMAILSLTGFLVLSAGCKTEAKVTLPTVSTTEVTNI
                     TAHSATSGGNILDSKGMTVLTRGVCLAQTANPTINDRITIDGSGTGTFTSTPDLLTPG
                     TGYFLRAYATNKAGTAYGNSVTFTTKSETTNTGFAKGADIGWLQQMEATGYKFYNDAG
                     VQTDCIQILKDHGINSIRLRTWVNPSNDKASGHCSKEETVAMAVRAKNAGMRVMINFH
                     YSDSWADPSKQVKPAAWKDLTFEQLKQALYDYTVDVMSALKTAGVTPEWVQVGNEIPT
                     GFLYPEGSIYKADGKTEDWAPMVQLLNKGYDAVKFVSPDTKVILHVDRGNDNGRFRWW
                     FDAVTKQNAKYDIIGMSYYPYWLDGKPDYTLSINALGNNLNDMAQRYGKEVMVVEVGG
                     EDGQDTQLTAAQNAAKIQNTYDMLVAVLDKTRAVPNGKGLGVFYWEPQGARSWSYYPL
                     SAWGNDGKPTHAMDAFLK"
BASE COUNT         3934 a         2923 c         2837 g         3864 t
ORIGIN      
        1 ctggaggcga ataatcatca tgccatttcg tatggcgatg atacaattac tgaagaaacc
       61 aaccggcttt ttgagaaaat attcggtgaa gtgaaagtgc tctatgcatt caatggtaca
      121 ggtgccaatg tggtagcact caaatgttgc acattgcctt tccaatcggt gatatgtgcc
      181 gaaactgctc acatacaggt agatgagtgc ggtgctccta cgcagagcat cggttgctcg
      241 ctggtggcac tacccacacc cgatggaaag ctcactcccg aactcatccg gccggaacta
      301 aaacaccgag gtaatctgca ccacacacaa cccaaagtga tttcaattag tcagactacc
      361 gaactgggaa ccatttacag ccccgatgaa ttgaaagcgc tgagcaattt tgcacacgaa
      421 aatgacctgt acctgcatgt ggatggtgcg cgtatatcca atgctgtagc cgctcttggc
      481 tgcagcctga aggaagccac tgtggattgc ggcattgata tcatgagttt tggcggaact
      541 aaaaatgggt tgatgatcgg tgaagccatt ttgattttca ataaagaact gtggaaaaat
      601 gctccctacc tgcacaaaca atcggctcag cttttctcca aaaaccggtt tattgcagca
      661 caattcaaag ccctgcttga aaatgaattg tggcgtacca tggcgcttca ttccaacaag
      721 atggctcagc tgctgaaaaa tgaaattata aagataaaag gtgtaatcct tacacaagat
      781 gttcaggcca actcgctttt tgttattatt ccggctcatg ctgtagagcc actacgagaa
      841 gcatatcgct tttacgactg gaataccgca accggtgaac tgcgctggat gtgttcgttt
      901 gacactacag aagaggatgt tctcaatttt gtgaccactt taaaaagcct tttgtaaaca
      961 aaacatatcc ctaatacatt atattccaaa cttaaagcaa aaaaaaccaa catatgatta
     1021 ccaaagaact ggaaaaatat atatcgacaa ttccaaaaac ggagcttcat ctccacattg
     1081 aaggttcgtt tgagcctgaa ctgatgtttg aaattgctca gcgcaatcag gtaaacatca
     1141 aattcaaaac tgtagaagaa cttagacaag cctacgagtt tggtaacttg caggattttc
     1201 tggacatata ctatgatggt gccaatgtat tgctacacga acaggatttc tacgacctga
     1261 ccatggctta tttccggaaa tgtaaacagg ataacgtggt acataccgag atatttgccg
     1321 atcctcagac ccatttatcg cggggtgtaa gcatgaaaac cataatcgac ggcattacgc
     1381 gtgcccgccg ggatgccgag cgcgaatttg gcatcagttc ttatctgata gtctgctttc
     1441 tgcgccatct ggatgaaaat tcggccattg aaatgttcga agaagccctg caattcaggg
     1501 ataaaatcat aggtgtggga ctggattcat cggaagtggg caatcatccc gaaaaattca
     1561 aacgggtatt tgcccgggct cgggaagaaa acttcaggct ggtggctcat gccggcgaag
     1621 aaggaccttc cgattatatt tggggagctc tcagggaatt gaatgtaagc cggattgacc
     1681 acggtatacg gtgtatgggc gacgaagagt tggtggaata tctggcggaa aatcagatac
     1741 cgctcaccgt gtgtccactt tccaacctca agttacaggt ggtaaaaagt ctgacacacc
     1801 acccgctcaa agagatgctt gacaacgggc tgctggcaat ggtcaattcc gatgatccgg
     1861 cctatttcgg gggctatgtg aatgaaaaca tgctgcaaac tgccgatgcg ctcaatctgt
     1921 cggagaacga tattctgcaa ctggccaaaa attctttcat tgcatccttt ctacccgacg
     1981 aagaaaaaga aaaatggttg acggaactgg ataattttcg aatttccaat tgagaataaa
     2041 tttgcaatca actgaaattc aatattttat catttcaata aaaataacga aatagcagga
     2101 attaattatc actgcaacct ttctttcaaa aacggataat cctggattaa tccgtttttc
     2161 tttttttcca cactgagctg aaagagccgg tattcttcag tttcggggtg cagagggcgg
     2221 tgattgagat cggcccgcaa ccgttcaagc tcctttttca aattcagtgt ttcggacgag
     2281 aaatatgtat cgccaaaccg ctgccagata taatcaacgg ccacatcgga cggatgaagc
     2341 atgtcggatg cataaaagcg gtaatcgcgc agttcatcca tcattatttc atacgcagga
     2401 aaataatgca cgaaatcaaa ctgtttttcc aatgcatcca ccgccagatg taaggtactt
     2461 ttactcaccg tattttcatg cgcaccatct ttccagtgac ggatggggct cactgtgaaa
     2521 ataaacttca gaccgggata gctgttgcgc agtaaaccga acacttctgt gaattcatta
     2581 ataatctcat ccacactcag acgccttctg gtgaaccggc tggcgggcag tttatgacaa
     2641 ttggttacaa tttttcgggt atcaaccaat tcaaaaaccc aggctgttcc cagtgttatc
     2701 atcatcacat ttgtttcgtg cagaaaatac cgtgcagcct gcaagcgact gtttattttt
     2761 tgcaatgctt catcggctgt aacagccgaa aaagtgctgc tgtgagccag actattccag
     2821 agcgaacccg actgaaataa atctgcggct gtgaattcct tctcactcaa caaatgccga
     2881 atgccctgtg cgaccgacat cggattatac atgaccccaa acggattgat aaaagtcaga
     2941 aaaaaagctt cctgcagttt tgtgcctata ttttcggcaa agcaggagcc caacgttaaa
     3001 atcctgtcgt tataagtgat ttccactgcg gaatgaggaa tgtctatctg tgtagaaaac
     3061 atattaattc aatttatggc attaatcatc cttacaaaaa tacaatattt atcggtatga
     3121 aataacaaag ccgcaacaga aagtcacggc tttggaattg aaattacccg tttctgaata
     3181 attatttctt ggcaagcgtt gttttttttg ctgttttttt gattgcggca gcttttgtag
     3241 tttttacagc tttatcttta cagcagtcag ctttatcnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnngct ttatcagctc cggccattgc ctttccgtct ttgcagcaag
     3421 cagctttatc ggcacctgcc attgctttac cgtctttgca gcatcctgct ttgtcggcac
     3481 ctgccattgc tttgccgtct ttgcaagcgg ctttatcagc tccggccatt gctttgccgt
     3541 ctttgcagca tcctgctttg tcggcacctg ccattgcttt gccttctttg cattcatgtt
     3601 ttgccattgc ctgaccctga gcacattcgt gttttttcat tgaatcggct ttggtacatt
     3661 ctgttttggc ctgagtgcat tcgtgtttaa cttcagcgtt agcatttttg cattcgtgtt
     3721 tggctttatc agcacattct tttttctgag tttcggcggc attggtcacc acaggagttt
     3781 gagcggatat catggttgct gagatgaata cagcaaccaa cgtcatcatt acattttttg
     3841 ttttcattta ataaatggtt tttttagtta tttgtttata ctgattgttt gaatttttat
     3901 acaaagatat ttattttata tatttctgca agtttaaact taaaatttca ttttcggtta
     3961 ccaaccggag tttctgtttt cactacattt gcttcatatc ccaaatcgtt gaaagcctta
     4021 atcaacttac tttcattggt tttcagggta tcaaatttga tagttacggt tttatcttcc
     4081 agttttaccg ccatatcctt cacccctttt tcatatgcaa tgttcttttc tattttccgc
     4141 tggcagcttt cgcaatgcat ggaaacattg aacataacgg ttttctttga ttgatccttt
     4201 ttggtctgtg cctgaagatt aatgcttaac gcaaatacca ttaaaatagt tgtcaaaaca
     4261 tactttgttt tcatatttgt aatgtttaat taattttatt tttataattt atcctttcct
     4321 gtcaatgccc agcggaaacc taaatacaat gtccttccat gcatcgggcc ccaaaccatg
     4381 gatgcatcaa aatcagtgcc aaatggattt tgaacatcaa tgatcggatt atgctgcaca
     4441 aaaccggtga tattttcggc acctccatac accgaccagg ttttgaaatt tttggttacc
     4501 tgggcattca taatggtgaa tggcttaaat tcccgttccc aaagcggatg agccgggtcg
     4561 ggatcgggta aacgaccgcc accgttgaac tgcgtggtaa aatcgaactg ccactttcga
     4621 agcggtgttt gatacgataa ggatgccaga cttttactgc gattggttag tggtttctcc
     4681 cgcagattac cattgatggt tgttttcaca tcatttattc tgtgtgcgaa tgttgctgtc
     4741 aatcccctta cgggttctat attcgcttct atttgaaaac tgctggaata agacttgcca
     4801 cgcagattgc tgaagctgac agcatgtgga tttgtatcca tatcggccac cacctgattg
     4861 ttaaaatcgg tgtagaacca ctctcccatc aggctcagtt ccttaccgaa caacgggaaa
     4921 aatccatgga ctgtaaatcc gtaattccag gcattttcca tcttcaagtc accggcaata
     4981 tggagcgaac gattgcttgc tagcagataa ttattttcgg ccagtacatt gggtgaacga
     5041 taccctttgc ctgctgaagc ccgcaaatga acatgatcca ggatgttgta acggacgtga
     5101 aatcggggtg ttataaaagt cccatacacc gaattattgt caacacgaag tcctgccatg
     5161 gccgtcagtg catcttttgt aaatgtatat tcaccaaaaa tacccggaat atattctgta
     5221 cggttaaatt cccccggcaa agagttcaga ttgaggtttt cgtttattac atccatattg
     5281 aaactggctc cggtcgatat tttatgctgt gtcccaatgg aagcatcata aatcagattg
     5341 gcatacaagt tactctgatg tccgtcgtac tgtttcaatc cgtagcgggc atcctgattg
     5401 tggatggtgc cgttgaatat aatccccaga ctggattgat tgtgcttttc gaccggttca
     5461 ttttcagagt gattatgatg ctggtcatta tctccgaaaa gaaatccgtt tttcacaaag
     5521 aattcatatc gctcggtatt cactcctatt ttgtaggagt catcggttcg tccaccggtt
     5581 cttttctcgg aaagtccccg tacaaatgcc tgcagaatat accctccgga attgtaaaac
     5641 caacggttgg ccagattata ctgcttcacc ttcggcaaat ccatatatcc gtcacggtta
     5701 tcgtcgaggg tcatgaattc atcggaggca tgcatcaata ccgaagttgc cgttttctca
     5761 cttaatttta cagctacatc ggcgttggtt tccacccgac cggtactgtt tgtaaataaa
     5821 ttcagagaaa ccttgtcact ggtttgtggt tttttgtatt ctacatttat ctgaccggca
     5881 atggcttcgt aaccattttt aacagaggca gtcccttttg atatttgaat gctttccatc
     5941 cacggaccgg gaatatatcc cataccgtaa gccgatgaca agccacgcag gttgggtata
     6001 ttttccgtaa gcatctgcac atacgttccc gacagaccga gcagcttaat ctgtttggca
     6061 ccagtggccg catcgctgta cgacacatct actgatgcat tggtctcaaa actttcggac
     6121 agattacagc aggcagcttt acgcagttca tgcgtgttga gagtttctac attgaacaca
     6181 gccgtccggc ttttaatttt accaagacgc tgttgtgtaa cagtaacttc tttcagttgt
     6241 atcgcatcgt ccagtgttat ttccaactga tttgtttgtt tggattttga cacatcgacc
     6301 gtatcggaat tataagctac attgctaaat accagcaagt tgtttccctg ttccggattt
     6361 agctgaaaat gtccgtcggc attggtcacc acaccatcat tgttaccagc ccagcgaaca
     6421 tatacacccg gtatggcctc gtttaattta tctttcacgt atccgtgaac ctgagacaaa
     6481 agctgggttg ttattgaaat acagagtatt aagatgattt gttttttcat ttgtgagttt
     6541 gatgttaata atttgaatat aaataaagaa cagaacgaat atactgctgt atatctgcct
     6601 gttacacaaa attcaaatta ataaatcaaa tcagcaggat ggaattttcg gacagaatcc
     6661 gccttccggt gagtggaatg aagagttgag aattgtgctc aacggtggtt ttaatatcag
     6721 cagtttcgaa accggtaata aaatctgtcc aaagtgaact taaatcagcg tacaacagag
     6781 aatagtccat caaaaaatcc tcattgagtt gcatggaagg tacatccact gtcaggcggg
     6841 tcaaatcaca ccctttttca aggtcatgtc cacatatatt ctcaactgtg gtgtgttgat
     6901 gatgttccga accgtcggca tcgtggtgat gatgaataca ttcgcaactc atttcggcaa
     6961 ccttttcaat gcctgcttcg gcacaggtat tacagcagta actgattaag ttaaagccgc
     7021 tgcctgcaaa aagaaaaatg caggaaagca aaatgctgat aatgatatga gtaagcggtg
     7081 ttttcatttt tcggcaacaa atatacgatg attggtctga agttgtatgt tctaagcggt
     7141 tattttgagt tgttttaaat aaaattagta tcaatctctt gaaaaaatgc cggtcggaat
     7201 gttttatttg tattagaaaa agcaacttaa agcaaaaaaa gaaataaatt acctgaaaaa
     7261 gtagtaattt tgcacaactt aaaaaatagt gaattcattt atgaataaat atttactttt
     7321 ttctgcttca ttactgctta tactggtaaa ttcctgcacc aaacaggaaa tggaaatgaa
     7381 gaattacagc tatgccaata catttcacct cacccctgac accacgctgg gcgggctgag
     7441 cataagtata gaggcagaac ttcccgttaa ctttagtaat aaagaaattc ttaaaagcat
     7501 acaacaacaa atagtgggta aaatgtttgg tgaaatttac accaatctgc cggtcgattc
     7561 cattattcca tcgtatgtca gccgcttgta caacgactat aaaatcagct acggtaccga
     7621 tctggaaaaa tacaagaatc tgaaaggtcc gatgctgagt aacgaaattc aggtggaagc
     7681 ggtatccatg tttctggatg ataaaatact gtcgtacagc tacgagcgat atgcttacat
     7741 gggtggtgcc cacggcaaca gcaaccggta tttctacaat tttgatctga gaaacggtca
     7801 catcctgcac gaaagtgata tttttatcga taactacaaa gctcccctta ccgaactgat
     7861 aaaacaacgg atagtggaag ataatgcaga aatagaatct gtagccgacc tgactgaatt
     7921 tgatttttgg gaaagtgaaa ttaagccgaa cggcaatttt tatatttcgg acgaaggaat
     7981 tgtgtatgtc ttcaatccct acgaaattgc gccttacagt accggtcaga ccgaagtgac
     8041 gctgccctac gaccgactaa aggtcttgct taaacccgga aatctgattt catatcttta
     8101 ttctacacct gcttaaaaaa cgataaaatc actatgtcat ttcttgaatt gctgaaaaac
     8161 cgccggagca tccgccgata taccgaccga aaagttgaac ctgaaaaaat tcaacaaatc
     8221 cttactgccg cattgatgtc accatcatcc aaacggagca atccctggga attcattgtg
     8281 attgaagagg atgaaatgct ccaaaaactg tctgcatgtc gtcccaccgg ttcacagttg
     8341 cttgccggtt cacccctggg catagttgtg attgccgacc aaacccgaac cgatgtgtgg
     8401 atggaagatg catccattgc tgccattatt atgcagctgg aagctcatga tttgggactt
     8461 ggaagctgct gggtgcaggt gtggggtcgc cagaaagaag aaggagtaag tgccgaagca
     8521 tatataaaag aactgctgag tatacccgat gaatttgggg tgttgtgcat catctccatt
     8581 ggctaccgcg atgaggaacg caaaccttac gacacagaaa aacttgatta caataaaatt
     8641 catttcggaa agtttaataa acaaccctaa taattttttc aaactcaacc cacgatgaaa
     8701 gacaatcgtt tagcctgggg aatttccctt ttattatttg gcgtgatgtt tctgctgcgt
     8761 cagttaggca ttttctctcc cgaaatagcg gatgtacttt tcgatttcaa gaatttcccg
     8821 ctcataatcg gcgtaatatt tttgatttgg cacaaaaata aatccatcgg catgatactg
     8881 atagtggtag gtttattgct ccgcttgcag gaaatcattc actggacaca aaatctgtcg
     8941 gaattcgtat ggccactgct gcttatcgct gccggcatca ttctgctttt cggagttaag
     9001 aaagggaagt aaacgaattt cgaaccatta ttaccaaatc ggtgtatcat gaaaattgta
     9061 ttattgggtg ccggaaacgt tgctacacat ctgggtttac gcctgataca gaccggggtt
     9121 gaactcctgc aggtttacag tcgtactgca tcttcggcta cggaactggg caagctgttg
     9181 ggtgtgcctc attgctccga cctgaagcaa ctcgataaaa ccgctgatat ttatatttat
     9241 tcgattaagg acagtgcttt aagtgaagtt atttccggag ttaatatccc ggatgccata
     9301 cacctacaca ctgccgggag cattccgatg gatattttcg ggggttttgc tcatcattac
     9361 ggcgtactct acccactgca gacattttcc aaagccaaac cgttagattt cagtcaaatt
     9421 cccttattcc tcgaagctaa cgatgatcac accgccggga tactcactca actggccacc
     9481 cgactttcat ctgtacaata ctacctcagt tccgaaaaac gtaagaaaat acatctttcg
     9541 gctgtttttg catgcaattt caccaattat atgtaccgga ttgctgatga tatagttagt
     9601 gaaacaggat taccctttga cgtgcttcag ccgcttataa ccgaaacagc cgacaaaatc
     9661 cgatacctca ctcccactga agcacaaaca ggacctgctg tgcgcaacga tgaaccaact
     9721 atgctcactc acatcaatct gttgaaggag aatgaaaatg cactcaaaac atatattaca
     9781 ctaagcgaac aaattaaaga gctatacaaa aaataaatgg aagcatacct gcggattgta
     9841 cgatggaaaa acctgctctt tatcctggtg atacagtggc taatgcaaca tgctgtggtc
     9901 tatccgctgc tgcaaaccta tggttttgaa ataagccaac gcgatgtttt taatattttg
     9961 ttgtatgcag ccaccatgct gatatctgcc ggaggctatg tcatcaacga ttattttgac
    10021 gtaaaaattg accgtctcaa tcggcctgat aaagtgatag tgagtgagaa gattacacca
    10081 aaggcagcca tgctgtatta tcaaatgcta actgttacgg gagttatttg cggtctcatt
    10141 ttagccttcc tttctcatag ttttacgctg ggatttataa tgttatttac ccccggcttg
    10201 ctttggtttt actcagccag ttacaagcgt cagtttatta caggcaacgt tattgtcagt
    10261 ttttcagcat cgctggtcat gcttgtggtg ggttttctgg aggtagcttt acttcagata
    10321 caatacggta atctgctatt gcagacgcca atacctgcac aaatatatgg ctggaccggt
    10381 ggttttgcgg ccttcgcttt tgcatgcacc tggatacgcg aaatcatcaa agatatggaa
    10441 gacattcacg gcgacaggga aatggaatgc cggacaatgc ccataaaatg gggaatacct
    10501 aaaacaaaat actttctgta tgcgttaata atcctgacaa tagctgcgct tttccatgtt
    10561 gattttcact acattcattt tgatggtaat ctcactaccc gttatatcct gttcggtttg
    10621 acattgccgc tggttgcact gagttacctc attttcagaa gtaaaacccc tgaagatttt
    10681 catcaggcat ctacactgtc aaagtttatc atgctggcag gtgtgcttta cagtttgatt
    10741 ttttactatc ttacagcaaa aacatttcat atcagtattt tcggtttatt tattgtaaaa
    10801 tgaaatacat acttgaccat ctgagcaaat acgatatcat tttaggttct caatcgcccc
    10861 gccgacagga acttctggcc ggtctgaaca tcaatttcag ggttgaaagc atttatgtgg
    10921 aagaaaccta tcccgaaacg cttgtcgggg tggacattcc gatgtatctg gctcaaaaaa
    10981 aagcataggc tttcaccctc gaagaaaata cgatgctgat cactgccgac actattgtct
    11041 ggcacgaagg tgccgtgtat ggcaaacccg acgactggac cgatgcaaaa agaatgctat
    11101 taactctttc cggtaaaatg catcaggtta taaccggggt gtgtattacc acaaaaagga
    11161 aacgacgcgt ctttcacgta atttccgatg tgtattttgc ccgtttcaac gaagccgagg
    11221 ttgattatta cctggaaaat tatgagcctt atgataaagc cggttcatac ggtgtgcagg
    11281 aatggatcgg atatatcgga gttgaacgca ttgaaggatc ttacttcaat gtaatgggtc
    11341 tgccggtgca gcgtctgtac aacgaattga aaaactggaa agaatagtcc tatgaacaaa
    11401 gttacagttt gtatctcgcc ggcactttat tctttttatg cccaaaagga agctattgtt
    11461 atcatcgtgg atattttcag agccaccacc agtatatgtg ctgccttcaa caacggagcc
    11521 cgctcgatac acacggtagc cacggtggaa gaagccatac attacaaaga aaaaggctat
    11581 ctggtagccg cagaaaggaa tgtgaaaaaa tgcaacttcg ccgactttgg caattcaccc
    11641 tttgatttta gtcacgaaaa agtagccgat aaggaaataa tatttactac aaccaacggc
    11701 acacaagcgg tacaaatggc tctggatgca gatgaaatcc tgatcggggc attttcaaac
    11761 ataaccacac tcaccaatta ttgtaattcg aagaaaaaaa acgtactgat actatgctcg
    11821 ggttggaaca acaggttttg cttggaagac accctgtttg ccggtgcgct gacagagaaa
    11881 ttgataaaaa cagggaaatt ttgctccgat tcagatgcaa cacttgctgc gcttgaactt
    11941 tggaaaattg ctcacccgga tttaataaac tatatttgta gcaccgaaca ttatcaacgg
    12001 ctaaaaaaca acggtcttga ggattcggta gaatactgcc ttacgaacga cactactcca
    12061 gtattgcctg tttatgataa aataaataga atttttattc tcaaaaaata actgaaccat
    12121 gaagttacaa cattcaatcc aacgaatggc aatcctttca cttacgggat ttttagtctt
    12181 gagtgcagga tgcaaaacgg aagcaaaagt cactcttccc actgtttcaa caactgaggt
    12241 taccaacata acagcccatt cggccacatc gggtggcaac atcttggaca gcaaaggcat
    12301 gaccgtactg acaaggggtg tttgtttggc acagactgcc aatccgacta tcaatgaccg
    12361 gataaccatc gacggaagtg gtacgggcac tttcaccagc actcctgact tacttacacc
    12421 gggtactggt tatttcctca gagcgtatgc cactaataaa gccgggactg cttatggaaa
    12481 ttcggttaca tttacaacga aaagtgaaac aacaaatacc ggatttgcca aaggagcaga
    12541 tatcggatgg cttcagcaaa tggaagctac gggatataaa ttctacaacg atgcaggtgt
    12601 ccagaccgat tgcattcaga tactgaaaga tcacggtatc aattccatcc ggctgcgaac
    12661 ctgggtaaat ccttcgaacg acaaagccag cggacattgc agcaaggaag agaccgtagc
    12721 aatggctgta agggctaaaa acgcgggaat gcgggtgatg attaattttc attacagcga
    12781 cagctgggcc gatccatcca aacaggtaaa acccgccgca tggaaagatc tcaccttcga
    12841 acaactgaaa caagctctct atgactacac agtggatgta atgagcgcac taaagacagc
    12901 cggtgttacc cccgaatggg ttcaggttgg caacgaaata ccgaccggat ttctttatcc
    12961 ggaaggcagt atttacaaag cggatggcaa aaccgaagac tgggcaccaa tggttcagtt
    13021 gctgaataaa gggtatgatg ccgtgaaatt tgtaagtccc gatactaaag tgatactgca
    13081 cgtggacagg ggcaacgaca acggacgctt ccggtggtgg tttgatgctg ttaccaaaca
    13141 gaatgcaaaa tatgacatta tcggcatgtc gtactatcct tactggctcg atggaaaacc
    13201 ggattacacc ctgtcaatca atgcgttggg caataatctg aatgatatgg cacaacgtta
    13261 cggcaaggaa gtaatggtgg tagaagtagg cggagaggac ggacaagata ctcaacttac
    13321 tgctgcacag aatgcagcca aaatacagaa cacctacgat atgcttgttg cagtactcga
    13381 caaaactcgc gcagtgccca acggtaaagg tctcggggta ttttactggg agccgcaggg
    13441 tgcacgctcc tggagctact accccctcag tgcctgggga aatgatggca aacccacaca
    13501 cgccatggat gcattcttaa aataaaataa cgttaaaagc aggaacaata acagctttca
    13561 atctgttaaa aataacctga atgtaaataa aaaactctga gttttttatt catttagaaa
    13621 ggtcgaatta ttaacaatta aaaatatagg agataaaa
//
LOCUS       DAOPZK010000051        12983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_5315_length_12983_cov_23.034189, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000051 DAOPZK010000000
VERSION     DAOPZK010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12983
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5315_length_12983_cov_23.034189"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1012)
                     /locus_tag="PKH68_04695"
     CDS             complement(20..1012)
                     /locus_tag="PKH68_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNX12269.1"
                     /translation="MKKVLSMGNALTDILFQINNDEILSGLNLKKGSMQLVDAEKSDE
                     ITALFNDFPTTMATGGSAANTIKGIAKLGLEGGFIGKVCDDNIGSFFREDSRKNGIKP
                     HLKFSNTASGHCTVLISPDGERTLCTYLGAASELSAGDLESSVFTGYDIFHIEGYLVQ
                     DHNLIRTALQLAKKAGLTVSLDLASFNVVEENREFLHEIIRDYVDITFANEEEAKAFT
                     GKNPFEALTDIAHLCDIAIVKLGKDGSLIKSGEKTDEVIPSGDIRVDSTGAGDMYAAG
                     FLYGLARGYALNICGMIGSLISGRVVQVVGTTMSEETWNEIYAEIDDILKMETK"
     gene            1106..1179
                     /locus_tag="PKH68_04700"
     tRNA            1106..1179
                     /locus_tag="PKH68_04700"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1140..1142,aa:Asn,seq:gtt)
     gene            complement(1326..3788)
                     /gene="pheT"
                     /locus_tag="PKH68_04705"
     CDS             complement(1326..3788)
                     /gene="pheT"
                     /locus_tag="PKH68_04705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNX12270.1"
                     /translation="MNISYNWLKNYININVSPEQISKILTSIGLEVGAVEKIQTVKGG
                     LQGLVVGEVLSCNRHENSDHLSVTTVNVGEGEPLQIVCGAPNVAAGQKVIVATVGTKL
                     YSGEESFTIKRSKIRGVESLGMICAEDEIGIGTDHNGIMVLPADTKVGTKAKDYFGVK
                     DDTLIEVDITPNRIDAASHYGVARDLAAYFALTDDTVKLTKPSVDTFSVDNTNLSIPV
                     VVENPAACPRYSALTISGVTVAESPQWLKESLMTIGLRPINNVVDITNFILHELGQPL
                     HAFDADKITSGKVCVKNLPEGTPFTTLDGVERKLSGEDLMICNGDEPMCIGGVFGGLS
                     SGVTETTKNIFLESAYFHPVNIRKTARRHGLNTDASFRFERGCDPDITIYALKRAAML
                     IQEVAGGTISSEIVDEYPVIVQPFEVALNKEKIDNLIGKKIGNDTIVKILNGLEMEIV
                     SEDAEKFQLKVPAYRVDVQRDVDVIEDILRIYGYNNVEIGDKLISTLSYAAKPDSHKM
                     QILISEQLTAQGFNEVLNNSLTKGGYYSTLKDYPETQSVKIINPLSSDLNVMRQTLLF
                     GGLENISRNINYRNADLKFYEFGNCYHFNADNKSADDHLKAYSEEYHLGLWVTGNKFS
                     DSWSGGTQKSSFFELKAYVQNIFLRMGFDFRSLKSEEYADDLFSEALAIRSARGVLLA
                     TYGIVNHQVRKMTDVDAEVYYADLLWNNILSELNISAVKYTELPKFPVVRRDLAFLLD
                     KNTTFAEIEKIAFETERKLLKKVSLFDVYEGKNLETGKKSYAVSFMLQDETKTMTDNV
                     IDSIMRKIQQNIENKLNAKLRS"
     gene            4072..5793
                     /locus_tag="PKH68_04710"
     CDS             4072..5793
                     /locus_tag="PKH68_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX12271.1"
                     /translation="MIKENFIRLYEDAFRRNWELPGFTDYGENHTMTYQEIAVGIAKL
                     HLLFEQCEVKKNDKIALCGKNNSHWAMVYLGAVTYGAIIVPILQDFNPNDIHHVVNHS
                     ESKLLFVSDNIWENLDEENFITLQAVFSLTDYRPVVLLNQPAPTLLEENESRENIISP
                     LHKSKLTKRHIEDLFAQKYPYGFNKDSVRYIDKSNAELVSINYTSGTTGFSKGVMTSG
                     NALAGNVTFAVGTGLIAPGFNIVAFLPLAHAYGCAFDFLASTCAGCHIHFIGRTPSPK
                     ILLKAFAEVRPNVIFCVPLIIEKIYKKQIQPLLARPSMRWVLSIPFLDQPVLAQIRKK
                     LVDAFGGNFSQIVVGGAPLNAEVEEFFAKIKFPFTVGYGMTECAPLISYSEAKDFIPK
                     SSGKMLKGIMEAKIDRPDPNTGVGEIFVRGENVMSGYYKNPEATEQILDTEGWLHTGD
                     LGTLDAEGNVFIRGRNKTMILSANGQNIYPEELEAKLNNMPFVMESLVIESNGKLIAL
                     VCPDYEAVDAEGLDQKALDDVMEDNRKLLNSMGAAYENVAKIQLYPNEFEKTPKKSIK
                     RYLYTTV"
     gene            5956..6126
                     /locus_tag="PKH68_04715"
     CDS             5956..6126
                     /locus_tag="PKH68_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12272.1"
                     /translation="MKKVFLFIAVAFAIASCAPKQAEQEKVMEDSVVVADTVAAPADT
                     TIATVDSVAPQM"
     gene            6375..6560
                     /locus_tag="PKH68_04720"
     CDS             6375..6560
                     /locus_tag="PKH68_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12273.1"
                     /translation="MKKVFLFFAVAAVFAACAPKQAEQTEQTIDSVATEVVDSTAAVV
                     DSAATAVVDSAATAVVK"
     gene            6723..7574
                     /gene="hisG"
                     /locus_tag="PKH68_04725"
     CDS             6723..7574
                     /gene="hisG"
                     /locus_tag="PKH68_04725"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNX12274.1"
                     /translation="MLRIALQSKGRLNEESMALLHEAGIKLEWGKRLLLVPAKNFPIE
                     VLFLRDDDIPQTVADGVADIGIVGENEFAEKDKKVNIAKRLGFSKCRLSLAIPKSAEY
                     VDLSWFNGKVIATSYPGILEKYLKENNISADIHVITGSVEIATGIGLADAIFDIVSSG
                     STLLTNQLKEVEVVMKSEALLIANPNLTEEKTEILNELLFRIQSVQLAEDKKYVLMNI
                     PTENLDKIVQVIPGMRSPTIMPLAKEGWSSLHAVISEKEFWDIIGKLKNLGAEGLLVI
                     PIEKIIL"
     gene            7720..8967
                     /locus_tag="PKH68_04730"
     CDS             7720..8967
                     /locus_tag="PKH68_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12275.1"
                     /translation="MYNKKIFIAAVLLLSTVQLSVSQNNTNSPYTRFGYGDVNESTAT
                     ELRGMGGVSLGNRSRNTISPLNPASYSSVDSLTFMFDIGGSARYSLFSDKDGSNKTFN
                     ANLEYLTMRFPIAKWLGVSAGLTPYSLVGYNFNQTDSLAMPVNQSYEDTKNVYYYTLF
                     NGSGGFSQVYGGLSANILNHIALGANAYYMFGEVNNYRNLIFNSSSTYQSVTYANKIK
                     ASDFRFRYGIQLYNTFAEKHDVVLGAIYEGKTKFNGKYSAIANDSTLNSVNGFELPQT
                     LGIGLNYTYNKQLTVGVDVTNQGWGKALFFNKTDSLVNTTKIALGAEYIPNPMGRKYT
                     DRIRYRIGFNTVNAYYHSGADKLPNNYTISLGVGFPTKTSKSMMNASLEYGKMGSSLL
                     KENYLKLTFSASINEFWFVKPKL"
     gene            8996..9616
                     /gene="lptC"
                     /locus_tag="PKH68_04735"
     CDS             8996..9616
                     /gene="lptC"
                     /locus_tag="PKH68_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNX12276.1"
                     /translation="MKISKQIIINLTAAFWVVVVLFGAVSCEKERTEKIAAVKDRKTL
                     PGLHATKVTTVISDSGITRYRIYTDQWDVFDKAKESYWEFPKGLHFERFDENLAIDAN
                     FTSKYGRYYEGRRLWEFRGKVKAINVLGEMFESERLFWDQAQELIYSDTLVKITQLTR
                     VVSGYGFQSNQSMTKYSFRATLKGIIVLPPEEGNTMTSVPGNAARP"
     gene            complement(9623..10009)
                     /locus_tag="PKH68_04740"
     CDS             complement(9623..10009)
                     /locus_tag="PKH68_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HNX12277.1"
                     /translation="MNKSIIPLSPGLLAMLSKGASGIVPFRREIFLLEITVAGTTFCS
                     DIHKADKEIAPEKVLTMKREPDNKHDEFAIAIYVEDIRIGYVPAELNEVCSRLMDAGK
                     MFFCRVISKEWKKEWLRIKANIYMVE"
     gene            complement(10012..10809)
                     /locus_tag="PKH68_04745"
     CDS             complement(10012..10809)
                     /locus_tag="PKH68_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNX12278.1"
                     /translation="MTNSQISQTPENEALSTELSKLNTEFLELFTLHKDLVDEEPVIY
                     SLYLNRLGCLVFELFGKKTEYSRLKMKMELIQAALNRSQKPDLPEIESKLNNQLSDYY
                     KQIEENTGLLDEAKKVLTNLLPPEFVAELKEVFRVLCKKLHPDLNPDQPESDKELFLR
                     VKAAYDLNDLPELKKILLFIDSSSETQPTEQTADVKRQRIEFLKKNIESLKEKIQQLS
                     VTFPYNMKELIADDEKTAVEQEAIKNQISFYEKEIINYQAFIELMLS"
     gene            11033..11542
                     /locus_tag="PKH68_04750"
     CDS             11033..11542
                     /locus_tag="PKH68_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNX12279.1"
                     /translation="MSATVKLYSLNLSNVRTYLFAALFIIGNMLFPQLAHFIPGGGLM
                     LLPIYFFTLIGAYKYGLHVGILTAVLSPMVNSLLFGMPVLAVVPAIMFKSIILAVAAA
                     LAARYFGKISIWGILIAVLAYQVIGTGFEWILVSDFSVAIQDFRIGFPGMIIQLFGGY
                     LVLKALQKV"
     gene            11546..11947
                     /locus_tag="PKH68_04755"
     CDS             11546..11947
                     /locus_tag="PKH68_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNX12280.1"
                     /translation="MAELKTFDELMNRFREIEFHEKFDMIVAIANGGIIPAAIINQRL
                     NKEFRLLKINLRDAEQKPRYDAPQLISPVDFDCTGKTILLVEDRIKTGATVNFAITLL
                     KDAKLVKTFAVNGNADYSLYNETCFKFPWIV"
     gene            11935..>12983
                     /locus_tag="PKH68_04760"
     CDS             11935..>12983
                     /locus_tag="PKH68_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNX12281.1"
                     /translation="MDSLKRIFYFLLIASIFPSAIKAADIQRVDTIRLDEVIVTGSMP
                     KVNLRTLPMSISVVGERQIEERMQNSVLPLLTEEVPGLFITQRGVAGYGVASGAAGAM
                     SIRGIGGAPSTGVLVLIDGHPQYMGLMGHPLADSYQSMMTERVEVVRGPGSVLYGSNA
                     MGGVINIITKKQKRDGANLSFQSMYGSYNTLETQATGGIKQGRQNGNAGIGYNRSDGH
                     RKNMNFEQLTGYVRGGYDLSDNWKTSVDLDLSKSLASNPGTVEIPLNDNDGDVMRGVT
                     SVALENEYENTSGGLRIYYNFGEHKINDGYREGVTPPVYRFNSRDYMAGASLFQSYRL
                     MEGNRTTAGVDVQRFG"
BASE COUNT         3705 a         2646 c         2778 g         3854 t
ORIGIN      
        1 aaaaaataca gttttaagat tactttgttt ccattttcag aatgtcatca atttcggcat
       61 aaatttcatt ccaggtttct tcagacatgg ttgtaccgac cacctgtacc acccggccag
      121 aaatcagcga accaatcatg ccacatatat tcagggcata accccgggcc agtccatata
      181 agaagccggc tgcatacata tcgccggcgc cggtagaatc cacgcgaata tcgcccgaag
      241 gaataacctc atcggttttt tcgccgcttt taatcaaaga accatcctta cccagtttta
      301 caattgcaat atcgcacagg tgagcaatgt cggtcagggc ttcaaagggg tttttgccgg
      361 tgaatgcctt ggcttcttct tcgttggcaa aagtaatatc gacatagtcg cgaatgattt
      421 cgtgcagaaa ttcccgattt tcttccacca cattgaagct ggccaggtcc agagaaacgg
      481 ttaagcccgc ttttttggcc agttgcaagg ctgtacgtat cagattgtga tcctgaacca
      541 gataaccttc tatgtgaaaa atatcgtaac cggtaaacac ggaactttcc aaatcgccgg
      601 ccgacagttc actcgctgca cccagatagg tgcataaggt acgttcgcca tcgggagata
      661 ttaaaacagt acaatgccct gaagcggtat tgctgaattt caaatgaggt ttaataccgt
      721 ttttgcggct gtcttccctg aaaaacgagc caatattgtc gtcacatact ttgccgataa
      781 atccaccttc cagcccaagt ttagctatac ctttaattgt attggcagcc gaaccgccgg
      841 tagccatggt ggtgggaaaa tcattaaaca aagctgtaat ttcgtccgac ttttccgcat
      901 ccaccagctg catgctccct tttttcaaat taagccctga cagaatttca tcgttattta
      961 tctgaaacag tatgtcagtc agggcattac ccatagaaag aacttttttc atttttttta
     1021 tttcaattat ttctgcaaag atattgcata tttcaagaat tcctattatt tttgcactcg
     1081 caattgagaa attagttgca gaaattcttc cttagctcag tcggttagag catctgactg
     1141 ttaatcagag ggtccttggt tcaagtccaa gagggagagc aaaaaacact gaatgtcagt
     1201 gtgatagaaa aacaaaggac taaaagtggt gtcaaaaccg ccttagtcct tttctttttg
     1261 tgtcatattt aataaaaaaa aagctttcag aagaaagctt tttttatttt gaataatgaa
     1321 aagtcctaac ttctcagttt tgcattcagc ttgttttcaa tgttttgctg aatttttcgc
     1381 atgatgctgt caattacatt gtcggtcatt gttttggttt catcctgcag catgaagctt
     1441 acagcatacg attttttgcc ggtctcaagg tttttgccct catacacatc gaacagactt
     1501 acttttttca acagttttcg ttcggtttca aatgcaattt tctcaatttc ggcaaatgtt
     1561 gtgtttttat ccagcaggaa tgctaaatcg cgtcttacaa ccgggaattt tggaagttcg
     1621 gtgtacttta cggctgaaat attgagttcc gaaaggatgt tattccagag caagtcagca
     1681 taataaactt ctgcgtccac gtcggtcatc tttcgcacct gatgatttac aattccgtaa
     1741 gtggccagta aaacgcccct ggcactgcga atggcaagcg cttcggagaa taaatcgtcg
     1801 gcatattctt cgcttttcaa actgcgaaaa tcaaaaccca ttcgtaagaa tatgttctgc
     1861 acataggctt tcagttcgaa gaagcttgac ttttgagtgc caccggacca agaatcgctg
     1921 aatttgttgc ccgttaccca caatcccagg tgatattctt cggaataggc tttcaggtga
     1981 tcgtcagccg atttattatc agcattgaaa tgataacagt tgccaaattc atagaattta
     2041 agatctgcat tccggtaatt gatgttgcgg gaaatatttt caagacctcc gaataataag
     2101 gtctgacgca tcacattcag gtcagaactc aagggattaa ttatttttac gctttgcgtt
     2161 tcaggataat ctttcagggt ggaataatag ccgccttttg tgagtgaatt gttcagcact
     2221 tcattaaatc cctgagcggt cagctgttcc gagatgagta tctgcatttt gtggctatcg
     2281 ggtttggctg catagctgag tgtcgaaatc agtttatcgc caatttccac attattgtat
     2341 ccatagattc gcagaatgtc ttcgatcaca tctacatcac gttgcacatc cacccggtaa
     2401 gccggaactt tcaactgaaa tttttctgcg tcttccgata cgatttccat ttcaagtccg
     2461 ttcagaattt tcacgatcgt gtcatttccg atttttttgc caatcaggtt gtcaattttt
     2521 tctttattta atgcaacctc aaagggctga actataaccg gatactcgtc cactatttcg
     2581 ctcgaaatag taccgcctgc cacttcctga ataagcatgg cggcacgttt gagtgcgtaa
     2641 atggtgatgt cggggtcgca gccgcgttcg aagcggaacg atgcatcggt gtttagtccg
     2701 tgtcggcgag ccgttttacg gatgtttaca ggatgaaagt aagcactttc gaggaatata
     2761 tttttagtcg tttcggttac tcctgagctc aaaccgccaa aaactcctcc aatacacatg
     2821 ggttcatcgc cgttgcatat catcagatct tcgccgctta gtttgcgctc cacaccatcg
     2881 agagtggtga aaggtgtacc ttccggcagg ttttttacac ataccttgcc ggatgtgatt
     2941 ttgtcggcat cgaaagcatg cagcggttga cccagttcgt ggagtatgaa attggtaata
     3001 tccacaacgt tgttgattgg acggagacca attgtcatca ggctttcctt aagccattgc
     3061 ggagattcgg ccaccgttac acccgagatg gtgagtgctg aatatctcgg acatgctgcc
     3121 ggattttcta caacaacagg aattgacaaa tttgtgttgt caacgctgaa ggtgtcgact
     3181 gagggttttg tcagtttaac ggtgtcatca gtcagagcaa aataagcagc caaatcgcgg
     3241 gcaacgccgt aatgtgaagc ggcgtcaata cggttaggcg taatgtcaac ttcaatcaat
     3301 gtgtcatctt taactccgaa ataatctttg gcctttgttc cgaccttagt atctgcgggc
     3361 agcaccatga tgccgttgtg atccgtaccg atgccgattt catcttcagc gcatatcatc
     3421 ccgagtgatt ccacaccccg tattttggag cgtttgatgg tgaaactttc ttcgccggaa
     3481 tataattttg tgccaacagt ggctacaatc actttttgac cggcagccac attgggtgct
     3541 ccgcaaacaa tttgaagcgg ttcgccttct cctacattaa cagtggtaac gctcagatgg
     3601 tcggaatttt cgtgccggtt acagctaagc acttcgccaa ctaccaaacc ttgcagaccg
     3661 cccttcaccg tctgtatctt ttcgaccgca ccaacttcga gtccgataga ggtcagaatt
     3721 tttgaaattt gttcaggcga tacattaata ttaatgtaat ttttaagcca gttgtaagaa
     3781 atattcatac tatatataat attatgtctg tgtttaggaa ataaacggca ttagaattcc
     3841 atattccgct tatttcaagg tgcaaagata cggttttttt acagttagga aaatgaataa
     3901 aattcaaaat aacaataatc attaaaaaga tgcacacttt tggttgttgt gtgtattttg
     3961 attaattttg tcggctttta aaaatcgaaa tgccaactca tatcatttgt tctcacctgt
     4021 ttaatgttta ttttagtgat ttcaaatggg ttacttaaac tttttttagc tatgatcaaa
     4081 gaaaatttta ttcgactgta tgaagacgcg ttccgtcgta actgggaatt gccggggttt
     4141 accgattatg gtgaaaatca taccatgacc taccaggaaa tagcggtcgg aattgccaaa
     4201 ctgcacctgc tatttgagca gtgcgaggta aagaagaacg ataaaattgc attatgcggc
     4261 aaaaataact cacactgggc catggtctat ctgggcgcgg taacctatgg agctatcata
     4321 gtacccatac ttcaggattt caatcccaat gatatacatc acgtggtcaa tcactccgaa
     4381 tcgaaattat tatttgtaag cgataatatt tgggaaaatc tggacgaaga aaatttcata
     4441 actctgcaag ccgttttctc actgaccgat taccgtccgg tagtattgct caatcagcct
     4501 gcacctacgc tgctcgaaga aaatgaaagc cgcgaaaata taatttctcc gcttcataag
     4561 tccaaactaa ccaaacgcca tatcgaagat ctttttgcac aaaaatatcc gtatggcttc
     4621 aataaagatt cggtgcgcta tatcgataag tcgaatgctg agctcgtgtc catcaactat
     4681 acttcgggta ccaccggttt cagtaaaggg gtgatgacca gcggcaatgc actggccggc
     4741 aatgtgactt ttgcggttgg caccggtctt attgctccgg gctttaatat tgttgccttt
     4801 ttacccctgg cacatgctta cggttgtgct tttgattttc ttgcttcaac ctgtgcaggc
     4861 tgccatattc attttatagg gcgcacgcca tcacctaaaa tattactcaa agcttttgca
     4921 gaagtacgac cgaatgttat attctgtgta ccacttatta ttgaaaaaat ttataaaaaa
     4981 caaattcaac cgctgctggc gcgtccttcc atgcgctggg tactaagtat tccatttttg
     5041 gatcaacccg tacttgccca gattcgtaaa aaactggtag atgcattcgg aggcaatttc
     5101 tcgcaaattg tggttggagg tgctccgctg aatgccgaag tggaagagtt tttcgccaaa
     5161 ataaaatttc cgtttaccgt aggatatggt atgactgagt gtgctccatt gataagctat
     5221 tccgaagcca aagattttat tccaaaatca tccggaaaaa tgctgaaagg aataatggaa
     5281 gccaaaatag accggcctga tcccaacacc ggggtggggg agatatttgt gcgtggtgaa
     5341 aatgtgatgt ctggctatta caaaaatcct gaagctaccg aacaaatact ggataccgaa
     5401 ggctggcttc acacgggtga tttgggtacg ctggatgctg aaggtaatgt gtttatacgg
     5461 ggtcgcaaca aaactatgat actgagcgcc aacggacaaa atatttatcc tgaagagctc
     5521 gaagcaaaat tgaacaatat gccgtttgtg atggaaagtc tggtaattga aagcaatgga
     5581 aagctaattg ctctggtatg tccggattac gaggctgtag atgccgaagg gctggatcaa
     5641 aaagcattgg atgatgtgat ggaagataat agaaaattgt taaattccat gggtgcagcg
     5701 tatgaaaatg tggcaaaaat tcaactttat ccgaatgagt ttgagaaaac acctaaaaaa
     5761 agcattaagc gctatctgta tacaactgta taatattttt taagtgattg ataaacagga
     5821 ttatagctgc ggtaaatagt taaaatgaaa ataattacta aaaaaacgaa aaattatttg
     5881 tcagtgatta taaaaatgta gtaactttgc accgtttttg aaacacaact cactttaatt
     5941 tttttaattt agaaaatgaa aaaagtattt ttatttattg ctgtcgcttt cgctattgca
     6001 tcatgcgcac ccaaacaggc tgagcaggaa aaagtaatgg aagattctgt agttgttgcc
     6061 gatacagtgg ctgctcctgc tgatactacc atcgcaactg ttgatagtgt tgcgccacaa
     6121 atgtaaggaa gggtaaaacc tcactgactt gaattttatc tgtttggtga aacagattgg
     6181 aactgaaaaa ctgaaaataa acttattttg cacccctttt tagacttttt ttgaagaatt
     6241 cgacgaaaag gtgaaaaaaa aataaaaaaa aatcagaaat attaattgta tatcaaaata
     6301 atgatgtacc tttgtcacga ttttgaattg aacaaaatat ctaacatgtt aaatttttaa
     6361 aggattaaaa caaaatgaaa aaagtatttt tattttttgc tgtagctgca gtatttgctg
     6421 catgcgcacc taaacaagct gaacaaacag aacaaacaat cgattcagtt gctactgaag
     6481 tagttgactc tactgctgct gttgttgact ctgctgctac tgctgttgtt gattcagctg
     6541 ctactgctgt tgttaaataa ttttagctac cgcaaaaaat taattaaaac atctgtaaag
     6601 tccgttagtc tctaacggac ttttttattt gtcagaattg tagtactttt gttgtcatta
     6661 aatgtcggtt atccgtattt acgggcactc aaccattatt ctcaaaaaaa aataatccct
     6721 ccatgttacg aattgcatta cagtccaaag gtcgcctgaa tgaagaaagt atggctttac
     6781 tccacgaagc aggtatcaaa cttgaatggg gaaagcgtct gttgctggtt cctgcaaaaa
     6841 actttccgat tgaagtactt tttctgcggg atgacgacat accccaaacc gtagctgatg
     6901 gtgtggccga tataggcatt gtgggtgaaa atgaatttgc agaaaaagac aaaaaggtga
     6961 atatcgctaa aaggctgggg ttcagcaaat gccgcctgtc actggcaatt ccaaaatcgg
     7021 ccgaatacgt tgacttgagc tggttcaacg gtaaagtgat agccacctct taccccggca
     7081 ttctggaaaa atatctgaag gaaaataata tttcagctga catacacgta atcaccggtt
     7141 cggtagaaat agctaccggt attggtttag ccgacgctat ttttgatatc gtaagttccg
     7201 gaagcacgct gctgaccaat caattgaaag aagtggaagt ggtgatgaag tcggaagcgt
     7261 tgttgatagc caatccaaac ctgacggaag aaaaaacaga aattctgaat gaacttcttt
     7321 tcagaattca atctgttcag ttggccgaag acaaaaaata tgtattgatg aatatcccca
     7381 ccgaaaatct ggataaaatt gtgcaggtca ttcccggaat gcgaagtccc actatcatgc
     7441 cgctggccaa ggaaggctgg agttcgttgc atgccgtgat cagtgagaag gaattctggg
     7501 atattatcgg aaaactcaaa aatctgggtg ccgaaggact gctggttatt ccgatagaga
     7561 aaatcatatt ataagcccga tctgtgtacc aggttgcggc tcggtttttg gttttttaac
     7621 ggggttcaac tttatttatt ttcacaggaa aattaaaaat cccatctttg catatccaaa
     7681 aaattaagtt aatcggattg ctccgaacaa ataaattgaa tgtataacaa aaaaatattt
     7741 attgcagctg ttttgttgct aagcactgta cagttgtctg tgagccagaa caatacaaat
     7801 tcaccctata cgaggttcgg ttacggcgat gtcaacgaaa gtacagctac cgaattgcgg
     7861 ggtatgggtg gagtttcgct cggtaaccgg tcccgaaata cgattagccc tcttaatcct
     7921 gcgtcctata gcagtgtgga ttcgctcacc ttcatgtttg atattggcgg aagtgcccga
     7981 tattccctgt tttccgacaa agatggctcc aataagacct tcaatgcaaa cctcgaatac
     8041 ctcaccatgc gattcccgat cgcgaaatgg ctcggagtga gtgccgggtt gacaccctat
     8101 tcgttggtag gttataattt taatcagacc gactcgctgg ctatgccggt caaccagtct
     8161 tacgaagaca cgaaaaatgt gtattattac acccttttca acgggtccgg cggatttagt
     8221 caggtgtacg gcggactttc ggccaatata ttgaaccaca ttgccctggg tgccaatgct
     8281 tactatatgt ttggcgaggt aaacaactac cgcaatctta ttttcaactc ctcatccacc
     8341 tatcaatcgg tgacttatgc caacaaaatc aaggcatccg attttcggtt tcgctacgga
     8401 atccaactgt acaatacttt tgccgaaaaa cacgatgtgg ttttaggagc catatacgaa
     8461 ggcaaaacaa aatttaatgg aaaatattcg gctattgcca atgattccac cctcaattcg
     8521 gtgaatggat tcgagttgcc tcaaacactg ggcatcggat taaattatac ctacaataaa
     8581 caacttacag taggtgtgga tgttacaaat cagggatggg gtaaagccct gtttttcaac
     8641 aaaacagatt cgctggttaa tactactaaa atagctcttg gtgccgagta tattccaaat
     8701 ccgatgggaa gaaaatacac cgaccgaatt cgctaccgca ttggatttaa tacggtaaat
     8761 gcttactatc attcgggtgc cgacaaactt ccgaacaatt ataccatcag tctgggggtg
     8821 ggttttccca ccaaaacttc caaatcgatg atgaatgcat cgctcgaata tggtaaaatg
     8881 ggttcctcac tgctgaagga aaattacctg aaactcacgt tcagtgcttc catcaacgaa
     8941 ttctggtttg tgaaacctaa actgtaaatc ctttttgctt tccgatcact gaagcatgaa
     9001 aatttcaaaa caaataatta tcaatctgac agccgccttt tgggtggttg ttgtgttgtt
     9061 tggagctgtg tcgtgtgaga aggaacgaac cgagaagatt gcagcggtaa aggaccgtaa
     9121 aactttgccc ggacttcatg ccactaaagt aactacagtc atatcagact ccggaattac
     9181 ccgttacaga atttacaccg atcagtggga tgtattcgat aaagcaaagg aaagttactg
     9241 ggaattccca aaaggactgc attttgagcg atttgatgaa aatctggcca tagatgcgaa
     9301 tttcaccagt aaatatggcc ggtattatga aggtcgaaga ttatgggaat ttcggggaaa
     9361 agtgaaagcc atcaacgtgc tgggcgaaat gtttgaaagt gaacggttat tctgggatca
     9421 ggcacaggaa ctgatctact ccgatacact ggtgaaaatc actcagctca cccgcgtggt
     9481 atccggttat ggcttccagt cgaaccagtc catgaccaaa tacagtttcc gcgctaccct
     9541 caaaggaatt attgtgttgc ctcccgaaga aggaaatacc atgacttcag taccaggaaa
     9601 tgctgcccga ccataaacgg gattattcca ccatataaat attggcttta atccgaagcc
     9661 attccttctt ccattctttg gaaatcaccc tgcaaaaaaa catttttccg gcatccatca
     9721 accgggagca tacctcattg agctcagccg gcacatatcc gatgcgtatg tcctccacgt
     9781 atatggcaat ggcaaattca tcgtgtttgt tgtccggttc tcttttcatg gtgagtactt
     9841 tctccggagc aatttctttg tctgctttgt gaatatcgga acaaaaagtg gtgcctgcca
     9901 cggtaatttc gagcaaaaaa atttcgcgcc gaaaaggcac gatgcccgaa gctcccttgc
     9961 tgagcatggc cagtaatccg ggcgacagtg gaataatgga tttattcatt tctaactgag
    10021 cattaattca ataaaagcct ggtagttaat tatttccttt tcgtagaaac ttatctgatt
    10081 tttgatggct tcctgttcga ctgctgtttt ttcatcgtca gcaatcagct ctttcatgtt
    10141 atatggaaaa gtgaccgaca attgttgtat tttctctttc agactttcaa tgttcttctt
    10201 caggaattca atccgttgac gtttgacatc ggcagtttgc tctgttggtt gagtttcgga
    10261 gctgctgtct ataaagagta aaatcttctt tagttccggc agatcgttca ggtcataagc
    10321 cgcttttacg cgtagaaata actctttatc gctttccggt tggtccgggt tcaaatccgg
    10381 atgcagtttt ttgcacaaca cccggaatac ttccttcaat tcagctacaa actcgggtgg
    10441 aaggaggttt gtgagcactt tttttgcctc atcgagcaat ccggtattct cttctatttg
    10501 cttgtaataa tccgagagct gattgttcag tttactctct atttcaggca aatctggttt
    10561 ttggctgcgg ttaagggccg cctgtatgag ttccattttc attttcaacc gggagtattc
    10621 tgttttcttt ccaaaaagtt cgaatacaag gcagcccaat cggttcaggt acaaggaata
    10681 aattaccggc tcctcatcca ccagatcctt gtgcagggta aacagttcca gaaactcggt
    10741 attcagtttt gataattcag ttgagagtgc ttcgttttca ggtgtttgag aaatttgtga
    10801 gtttgtcatg aatatcgggg tattgtttta atccggttga tataccggtt tttcatttca
    10861 aagataataa atttgtccgg ttaaccttat tgattttgca gccaacggct taatatttta
    10921 taaaatcaat ccgttatcat ctgaatgttt gcgaagtttt tatagctttg tagatatctt
    10981 ttgagatttt attgttgaaa attattaata accaggttaa aaaataaaaa tcatgtcagc
    11041 aactgtaaaa ctttattcgc tcaacctcag caacgtacga acctacctgt ttgctgctct
    11101 gtttatcatt ggaaatatgc ttttccccca attggcacat tttattccgg gtggtggatt
    11161 aatgctattg cctatttatt tcttcacttt gataggtgcg tataaatatg gattacatgt
    11221 cggaattctc acagccgtat tgtcgcctat ggttaacagt ctgttgttcg ggatgccggt
    11281 gttagccgta gtgcccgcca tcatgttcaa atccataata ctggcagttg cagctgcact
    11341 cgcagcccgt tattttggta aaatatccat ttggggaatt ctgatagctg ttttggcgta
    11401 tcaggttatc ggtaccggtt tcgaatggat actggttagt gatttctcag tagccattca
    11461 ggatttccgc atcgggtttc cgggtatgat tattcagctc tttggcggtt atctggtgct
    11521 taaagcgtta caaaaagtct gaataatggc agaactaaaa acgttcgatg aattgatgaa
    11581 ccgtttccgt gaaatagagt ttcacgaaaa atttgatatg attgtggcta tagccaatgg
    11641 tggcattata cctgctgcca tcatcaatca gcgattaaac aaagaatttc gactacttaa
    11701 aataaatctg cgggatgcag agcaaaaacc gcgttacgat gcacctcagc tgatttctcc
    11761 tgttgatttt gattgtacgg gaaaaaccat cctgctggtg gaagaccgta taaaaaccgg
    11821 agcaaccgta aattttgcaa tcacactgct gaaggatgca aaactggtaa aaacttttgc
    11881 cgttaacggg aatgctgatt actcacttta taacgaaaca tgtttcaaat tcccatggat
    11941 agtttaaagc gaatattcta tttcttactt atagcatcaa tatttccttc agcaataaaa
    12001 gcggcagata tacagcgtgt tgacaccatc cggctggacg aagtaattgt tacaggttca
    12061 atgcccaaag tcaatctgcg aactttgccg atgagtattt ccgttgtagg agagcggcaa
    12121 attgaggaaa ggatgcaaaa ttcagtgttg ccattattga cggaagaagt acccgggctt
    12181 ttcattacac aacggggtgt agcaggttat ggagtagcct ctggtgccgc cggtgcaatg
    12241 agcatacgcg gtataggagg agcgccttcg acgggtgtat tggtattaat cgacggacat
    12301 ccgcagtata tgggtctgat gggtcatccg ctggccgaca gttatcagtc catgatgaca
    12361 gagcgtgtgg aagtggttcg gggaccggga tctgtgctgt atggttccaa tgctatgggc
    12421 ggagtgatca atatcatcac caaaaaacaa aaaagagacg gtgccaatct atcctttcaa
    12481 tcaatgtatg gaagttacaa cacgctggaa acacaggcaa ccggtggtat aaaacaagga
    12541 agacagaatg gaaatgcggg aatcggctac aaccgatcgg acggacaccg taaaaatatg
    12601 aatttcgaac agttaaccgg ttatgtccgg ggcggatacg atttatctga taactggaaa
    12661 acatccgttg atcttgattt gtcgaaatcg ttagcctcta acccgggaac agtagaaatt
    12721 ccattgaatg ataatgatgg ggatgtaatg cgtggagtga catcggtggc tctggaaaat
    12781 gaatacgaaa atacgtcggg agggctcagg atctattaca attttgggga acataaaatt
    12841 aacgacggtt atcgtgaggg ggtaactcca ccggtttatc gttttaattc ccgcgattac
    12901 atggcgggag catcgctctt tcagtcctac aggctgatgg agggcaaccg aacaacggca
    12961 ggagtggatg ttcagcgttt cgg
//
LOCUS       DAOPZK010000052        12887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_5371_length_12887_cov_22.990103, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000052 DAOPZK010000000
VERSION     DAOPZK010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5371_length_12887_cov_22.990103"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PKH68_04765"
     CDS             <1..1176
                     /locus_tag="PKH68_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HNX12282.1"
                     /translation="LGHRLLDQSQLEEQTLFLEWLLDHITANGIDVLLISGDVFDVGV
                     PAANAQKLYYDFLIRLHQSCCREVIITGGNHDAPSTLNAPKELLNALSIHVVGKAVDP
                     ISDEVFRLTVDNEELIVAAVPYLRDQDIRRAVAAETAEEIVNRYKTALINHFHDVALF
                     CEQMKETNTTVIAMGHLFAVGGRTSESEQSIYVGNLGDIGAEDFPAVFDYVALGHLHR
                     SQCVGGKEHIRYSGSPYMLSFSEVGQDKKIIQIETGSSFNIKEITVPEFRKIRQLEGT
                     VASCKTQLEKLDQENNPLIPWVEVILSDEKEAATGYHEINEFAEHIRTEVLKITVRDE
                     RKYDRLKSLVENAQHITSFKPLDVFKLRCEEMNIDLDEKPEILDAFNEILQVVSEQN"
     gene            1181..>2255
                     /locus_tag="PKH68_04770"
     CDS             1181..>2255
                     /locus_tag="PKH68_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNX12283.1"
                     /translation="MKILKIELQNINSLKCETPIVIDFEHPRFDDVGLFAITGPTGAG
                     KTTLLDAITIALYRNVPRFEKTGGKGGLEDVVSYGAYTAMARVTLEVQNQRYEAHWDI
                     RTATNSGRQLGKPVETVRFKNLSSGKILAETKTGCDAKIVEITRLNYEQFLRSVLLAQ
                     GEFAAFLSAKNTEKGLLLQQIAGDEIYRKIGETLKNRIFEEKRLLEQIKNRINTDDLL
                     NGEVTEQLEAEKVQLIKNTQQLNDDLKKTEELLKEYEQRDKLEQQKQKIEDEKNRLEL
                     ERIQHAAGFLQLERHEAAEPLQPTLTEVNRLEKEIEQKNNRIESLGLEIIKLTDELTS
                     ATEKVKTCKLKTGEYEQAAIDWQP"
     assembly_gap    2256..2355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2356..4641
                     /locus_tag="PKH68_04775"
     CDS             <2356..4641
                     /locus_tag="PKH68_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNX12284.1"
                     /translation="AKQNAAGNNIEIERIIDNQKTQQAKLDALNAEVKELQEQLNKSD
                     LDKLLNQNQALNAKKGLLGELIPLSATYHLLLENLDTDTIKITELEHKLKELSVQIQN
                     IQKDIETQTQLVSDAEELYEKDSYIVSLEAERKKLKQGEPCALCGSREHPLVVEYTEI
                     EISDSRKRLDERKTALKNMESLKGETDKEYAAALAGFNHITIQQKQNKEALENVIRNF
                     AEKNSGYQIGEKQIMAAALVNAEIELTKTDNLIKITQGIRKSKDDTLLLIKNLEENIS
                     GIKVDLTRLTAANAETEKLIGENIKTGSQIRATVEELELRLSGQLSECGLLLPETEQT
                     DAFIRQLENSVKIFNDNVNKKTEAENKIQQINIEINNINERFTVKTNEENQIRVSMDE
                     LNKQLLACTESRKMILAPEISTGEKRMELQQAIDLSKKEEDIAIKTLQDLEKNKAGLE
                     AEKISVAKETAGLGEKLDKQKSTLQEMIDRSIFVGRQELAATILPEETKALLLRKRQE
                     LNERTISSASLEKENTSELEKLEEKLKTRATEEETRLQAAALNDQKEKLQLRMGEISE
                     SIRKDNEIRTRNAQVVIEINNQELVCRKWIALMNVLGGSQDAFNTYVQRLTLKNLIDL
                     ANLHLYKLNPRYSLLLNPQYKTGEELNFRLTDHYQAGETRLVDTLSGGEKFLISLSLA
                     LGLSDLASNNVSIGSLFIDEGFGTLDSNTLETVISTLETLKAQGKMIGIISHVDSLKE
                     RIPVQIQVSKKSNGVSTVTIS"
     gene            complement(4757..7510)
                     /locus_tag="PKH68_04780"
     CDS             complement(4757..7510)
                     /locus_tag="PKH68_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNX12285.1"
                     /translation="MAKDEELNDAEEQKLSDEEAPDMETDMEDAGNEMEDEEVKTHSR
                     YKIPKITGDAVKHHLSGMYQNWFLDYASYVILERAVPDIYDGLKPVQRRILHSMKRLD
                     DGRYNKVANVVGHTMQFHPHGDASIGDALVQLGQKDLLIDCQGNWGNILTGDGAAAPR
                     YIEARLSKFALDTVFNPKITEWKPSYDGRNKEPVNLPVKFPLLLAQGVEGIAVGLNSK
                     ILPHNFNELIDASVAYLKGEDFVLYPDFQTGGRIDISRYNDGERGGAVKIRSKIGKID
                     NKTLVITEIPFGTDTSKLIESIIKASEKGKIKIRKVDDNTSANVEILIHLAPGTSSDK
                     TIDALYAFTQCELSISPNCCVIHDNKPRFMGVSELLRISTDHTRDLLEQELIIQRDEL
                     LDQLFFVSLEKIFIENRIYKDREFEESSNQDEVVAHIDSRLEPFKKDFIREVTREDIL
                     KLLEIKMVRITKFDTDKANDTIIGIQKKIKEIEYNLEHLVDYTIFWFENLKEKYGKNY
                     PRRTEIRNFDTIVAAKVVEANEKLYVNHDEGFIGTALKKDEFVCNCSDMDDVIIFFKD
                     GKYKVVKVAEKQFVGKNILYINVFKKNDKRTIYNAVYRHGKGGPYYIKRFAVTGITRD
                     KEYDITQGKDGSKVVYFTANPNAEAEVIRITLKPKPRLVKLVFEKDFSDIAIKGRQSM
                     GNMLTKNDVHKIALKQKGGSTLGGREVWFDRDILRFNYDNRGEYLGEFQSDEQVLVIS
                     TKGEYRTTNFDLSNHFEKDILIIEKFDPNKVWSVVLFDADQKFHYLKRFQLEPTAKPV
                     SFLGENPASQLILMSGEDYPRFEVTFGGSDAYREALVVDAEEFIGIKSYKAKGKRLTT
                     YEIEAINELEPVRFKTEEKTEEDNTSDENNEEPETSEENENIDEVDNQKIIDDITGQM
                     TLF"
     gene            7701..8291
                     /locus_tag="PKH68_04785"
     CDS             7701..8291
                     /locus_tag="PKH68_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12286.1"
                     /translation="MRLKFILPVIIGLCSVFVSAQTVENGKIGLTYSLNGGTSVLQPA
                     LDGAASHEITAANSIGLIYLKPINNWLEWETGLTYTLYKVTTTSSPMPEVTTRNSTLS
                     LIDIPIGVRAGFWKYFFANGGLLLDLDMMSSSSISSQSGVGLMLGLGLKYDFSFGGSL
                     FFNPYGKIHSLLPFGLDKYHDRILESGWKFGFTYKL"
     gene            8463..9686
                     /locus_tag="PKH68_04790"
     CDS             8463..9686
                     /locus_tag="PKH68_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX12287.1"
                     /translation="MYRQEQIAEIAENQTLEFLNKDTGIPREFLSSIPQIENFATIIT
                     GIRRCGKSTLLLQLFKNETDTVLYFNWEDIRIAGFETDDFVRLYKEIIKQKVNILFFD
                     EIQLVPKWEIFVHQLLREGFRVFITGSNATLLSKELGTHLTGRHLSVELFPFSFSEFC
                     QFTRQNADDEAVNDYLHTGGMPEFVRTRQSYIIQQLVEDIITRDIAVRYNIRDVEALR
                     QLTVYLMSNVGNIVSANKLAGMFGIKSASTILEYFSHLTNSYLVDFIPQFDYSLKAQT
                     RNPKKVYGIDTGLVSEISLSFSRNTGHIFENMIYNHLRMKYKEILFFNKKGECDFVIK
                     EKEKITQAIQVCLQLTDENFRREYDGLLEAMNYFNLDEGLLITKNETDVFQKDGKTIR
                     VVTARQFLSNSYHPV"
     gene            9793..11340
                     /locus_tag="PKH68_04795"
     CDS             9793..11340
                     /locus_tag="PKH68_04795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNX12288.1"
                     /translation="MASQEDVFKKLIAHCKEYGFVFPSSEIYDGLSAVYDYGQNGVEL
                     KNNLKKYWWDSMTLLHENVVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDLLAKYDEKIEKEVEKAAKRFGESFDPTMFRQTNPRVLENQAKRDEIHARFVK
                     ALNDSNLDELKQIIVDYEVVCPISGTKNWTDVRQFNLMFSTEMGSTADGAMKIYLRPE
                     TAQGIFVNYLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEEMKWFEFWKEFRLKWHKALGLGDQKYRFHDHDKLAHYANAATDIEFEMPFGFKEVE
                     GIHSRTDFDLGSHEKFSGKNIKYFDPELNQSYTPYVIETSIGVDRTFLSIMAAAYTEE
                     ELEGGDSRVVLKLPPVLAPIKAAILPLIKKDGLPEKAREIMNELKFDFKCAYDEKDSI
                     GKRYRRQDAIGTPFCITVDHQTLEDNTVTIRHRDDMTQERVAITDLQGIIGDYVNMKN
                     LFRKIAD"
     gene            complement(11595..12725)
                     /locus_tag="PKH68_04800"
     CDS             complement(11595..12725)
                     /locus_tag="PKH68_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX12289.1"
                     /translation="MKTPVNPFITSGYLSPEYFCDREAETENLIHELINGNNVALIST
                     RRMGKTGLVKHVFNMEQMRKDYLTFFIDIYATKSLREFILALSKEIIERLKPLGQKAF
                     EGFISIVKSLQAGISFDVAGVPSFNVGLNDIVSETATLDELFQYLMNAGKPCIIAIDE
                     FQQIAAYPETNVEALLRTHVQHCSNACFIFAGSQRHTMGNMFLSASRPFYQSVSMIYL
                     EAIPVGKYITFAQKHFEKNKKKISSQAVIEIYDQFEGITWYVQKLLNMLFSNTQSGET
                     CDVDMIPAAVDQVVNSFRYVYAEILFRLPEKQKELLIAIAKAKDAEAITSAEFIRKYK
                     LTSASSVQAAQRVLLEKDYITNENGKYKVYDLFFRMWIVENC"
BASE COUNT         3946 a         2751 c         2739 g         3351 t
ORIGIN      
        1 ctgggtcacc gcctgctcga ccaatcgcaa ctggaggagc aaacgctttt tctggagtgg
       61 ctgctggacc atattacagc taacgggatt gatgttctgc tcatatcggg cgacgtattc
      121 gatgtgggtg tacctgcggc taatgctcag aaactgtatt atgattttct gatacggctt
      181 catcaaagtt gttgccgtga agtgatcatt accggtggaa atcacgatgc accgagcaca
      241 ctgaatgccc caaaagaact gctcaatgca ctttccattc atgtggtggg taaagccgtt
      301 gacccgataa gcgatgaggt tttcaggctg acggtcgaca atgaggagct gatagtggct
      361 gcggttccct acctgcgcga tcaggacatc cggcgggcag tggctgccga aacggcggaa
      421 gaaatcgtga accgctacaa aacggcttta atcaaccatt tccatgatgt agccctattt
      481 tgtgaacaaa tgaaagagac caacactact gtaattgcca tgggacattt atttgcagtg
      541 ggaggccgca cttcagagag cgaacaaagc atctatgtgg gtaatctggg agatatcgga
      601 gccgaagatt ttccggcagt attcgattat gtggcgcttg ggcacctcca ccgttctcaa
      661 tgcgtaggtg gcaaagagca catccggtat tccggttcgc catatatgct gagtttcagc
      721 gaagtcggtc aggacaaaaa aattatccag atagaaaccg gtagttcttt caatatcaaa
      781 gaaataaccg ttcctgagtt tcgaaaaata cggcaactgg agggcactgt ggcgagttgt
      841 aaaacacagc tggaaaagtt ggaccaggaa aacaatccac tgataccgtg ggtggaagta
      901 attctgagcg acgaaaaaga ggcagctacc ggttaccacg aaataaatga atttgctgaa
      961 catataagaa ccgaagtgct gaaaataacg gtcagggatg aaagaaaata cgacagactg
     1021 aaaagtttgg tggaaaatgc tcagcacatc accagtttta agccgctgga tgttttcaaa
     1081 ctcaggtgcg aagaaatgaa tatagatttg gatgaaaaac cggaaatact cgacgctttc
     1141 aacgagattt tgcaggttgt ttctgaacaa aattaaggac atgaagatac ttaagataga
     1201 attgcagaat atcaactcac tgaagtgcga aaccccgatt gtgattgatt ttgagcatcc
     1261 gcgatttgat gatgtgggtc tgtttgccat caccggacct accggtgccg gtaaaaccac
     1321 actgctcgat gccattacca tagccctgta ccgcaacgtg ccgcgttttg agaagacggg
     1381 tggtaaaggt ggcctggaag atgtggtaag ctacggagca tatacggcta tggccagagt
     1441 aactctggaa gtgcaaaacc aacgctatga ggcgcactgg gatatcagga cagccacaaa
     1501 cagcggccgt cagctcggaa aaccggtgga aaccgtacgc ttcaaaaatc tgagcagcgg
     1561 gaaaattctg gcagaaacca aaacaggatg tgatgcaaaa attgtggaaa tcacccggtt
     1621 gaattacgaa cagtttctac gctcggtatt gctggctcag ggtgaatttg ccgcttttct
     1681 ttcggccaaa aatacggaaa agggattgct tctgcagcaa atagccggtg atgaaattta
     1741 ccggaaaatt ggtgaaacgc tgaaaaaccg aatttttgag gagaaaaggc tattggaaca
     1801 aatcaaaaac cggataaaca ccgacgatct actcaatggg gaagtaactg aacaactgga
     1861 agcagaaaaa gtccaactca ttaaaaatac acagcaactg aacgacgatc ttaaaaaaac
     1921 ggaagaactg ctaaaagaat acgaacagcg tgataaactt gaacagcaaa aacagaagat
     1981 tgaggacgaa aaaaatcggc tggaacttga aaggatacaa catgcagctg gttttcttca
     2041 actggaaaga cacgaggcag cagaaccgct gcaacccacg ctgaccgaag taaaccgact
     2101 ggaaaaggaa attgaacaaa aaaacaaccg aatagaatcg ctggggctgg aaataatcaa
     2161 acttactgat gaactgacat cggccactga aaaggtgaaa acctgcaaat taaaaaccgg
     2221 tgaatacgag caggcagcca tcgactggca gcccannnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnngccaa acaaaacgca gccggaaaca atatcgaaat tgaaaggatt
     2401 atagacaatc aaaaaacaca acaagctaag ctggatgcac ttaacgctga ggtcaaagaa
     2461 ctgcaggaac agctcaacaa aagtgatttg gataaattgt taaaccaaaa ccaggctttg
     2521 aatgctaaaa aagggttgtt gggcgaatta attccacttt cggccactta ccatctactt
     2581 cttgaaaacc tggacacgga tacgattaaa atcacagaac tcgaacacaa actgaaggaa
     2641 ttatccgttc aaatccaaaa cattcagaaa gacattgaaa ctcaaaccca actggtttcc
     2701 gatgcggagg aactctatga aaaagacagc tacattgtca gcctcgaggc agagcgaaaa
     2761 aaactgaaac agggtgaacc ctgcgctttg tgcggttcca gagaacatcc gctggtagtg
     2821 gaatatactg aaattgaaat ttcggacagc cgaaagcgcc tcgacgaacg caaaactgca
     2881 ctgaaaaata tggaatccct gaaaggcgaa accgataagg aatacgccgc agcgttagcc
     2941 ggcttcaatc atattaccat tcaacaaaaa caaaacaaag aagcgcttga aaatgtaatc
     3001 aggaatttcg cggaaaaaaa ttcaggatat caaatcggag aaaaacagat catggcggct
     3061 gccctggtaa atgccgaaat agaactaaca aaaacagata atcttatcaa aatcacgcaa
     3121 ggtatccgaa aaagcaaaga tgacacatta ttactgataa aaaatctgga agagaacatc
     3181 tccgggatta aagtagatct aacccgcctg actgctgcca atgctgaaac agagaaattg
     3241 atcggtgaaa atattaaaac aggaagccaa atcagagcaa ctgtcgaaga gttggaactc
     3301 aggctgtctg gtcagttgag cgaatgtgga ttactgttgc ccgaaacgga gcaaaccgac
     3361 gctttcattc gccaactcga aaacagcgtg aaaattttca acgacaatgt aaataagaaa
     3421 acggaggctg aaaataaaat ccaacagata aacattgaaa tcaacaatat caatgagcgg
     3481 tttacggtta aaactaacga ggaaaaccaa attcgggtga gtatggatga actgaataag
     3541 cagctcctgg cctgtaccga aagccgaaaa atgattttag ctcccgaaat ttcgaccggt
     3601 gaaaaacgaa tggaactcca gcaagccatt gatttatcaa aaaaagaaga agatattgct
     3661 ataaaaactc ttcaggattt agaaaaaaat aaagccggac ttgaagcaga gaaaataagt
     3721 gtggcaaagg agacagcagg acttggtgaa aaattggata aacagaaaag caccctgcag
     3781 gagatgatcg accggagcat ttttgtcggt cgccaagaat tggcggcgac aatactcccc
     3841 gaagaaacaa aagcccttct cctccgaaaa cgacaggagc tgaacgagcg gacaatttcc
     3901 tccgcttcac tcgaaaagga aaatacctcc gagctggaaa agctggaaga aaaacttaaa
     3961 acccgggcga ccgaagaaga aaccaggctg caggcagctg ccctcaacga tcaaaaagaa
     4021 aaactgcaac tgaggatggg tgaaatatcg gaaagtatca gaaaggacaa tgaaataaga
     4081 acgcgcaatg ctcaggtggt tattgaaatc aacaatcagg agctcgtttg ccgcaaatgg
     4141 attgcgctga tgaatgtgtt gggtggttcg caggatgcat tcaatacata cgtgcaacgg
     4201 ctcacgctca aaaacctgat tgatctggcc aatctgcatc tatataaact caacccacgt
     4261 tactccctgt tgctcaaccc acaatacaaa acgggcgaag agctcaactt caggctaaca
     4321 gaccattatc aggccggcga aacgcgcctt gttgacaccc tgagtggtgg tgaaaaattt
     4381 ctgattagtc tctcgctggc actggggctg tcggatctgg caagcaataa tgtaagcatc
     4441 ggctccctct ttatcgacga aggtttcggt acgctcgaca gcaacacact cgaaacagtg
     4501 atttcgaccc tcgaaaccct caaggcacag ggaaaaatga ttggcatcat ttctcacgtc
     4561 gatagcctca aagagcgaat tccggtacaa atacaggttt cgaaaaagag caacggagtg
     4621 agtacagtca caatcagttg aaaaaaactg cacgagaact gtaaaacgga atgaaataaa
     4681 ttcttaaaaa aaacaaccac ctgaaaatcg tgtgatattt caggtggttg tattatttta
     4741 aaacatctga aatcatctaa aacagtgtca tttgcccggt gatatcatca atgattttct
     4801 gattatcaac ttcgtcaatg ttttcgtttt cttccgaagt ttcgggttct tcgttattct
     4861 catcggaagt attatcctct tctgtttttt cttccgtttt gaagcgaacg ggttccagtt
     4921 cgttgatggc ctcaatttca taggtagtca ggcgctttcc tttagcttta tagctcttaa
     4981 taccgataaa ttcttcggca tccaccacca gcgcttcccg ataggcatca gaacctccga
     5041 aagtcacttc gaaacgcgga taatcctctc ctgacataag tatcagctgc gaggccggat
     5101 tttcgcccag gaaactaacc ggtttagcag taggttcgag ctggaaacgt ttcagataat
     5161 gaaatttctg atcagcatca aaaagcacta ccgaccaaac cttgttggga tcaaattttt
     5221 cgataatcag gatatccttt tcgaaatgat tgctcaaatc aaaattggtg gttcgatatt
     5281 cgcctttggt agaaatcacc aacacctgct cgtcgctctg aaactcaccc aggtattcgc
     5341 cgcggttatc ataattaaac cggagaatat cgcggtcgaa ccaaacttcg cgtccaccca
     5401 gcgtggagcc gcctttttgt ttcagggcaa tcttgtgaac atcgttcttg gtcagcatat
     5461 tgcccatgga ctgacgccct ttgatggcta tatcgctgaa atccttttcg aaaacaagtt
     5521 tcaccaatcg cggttttggc ttaagggtta tgcggattac ttcggcttcg gcgtttggat
     5581 tggcagtgaa ataaaccacc ttcgaaccat ctttaccctg cgtaatatcg tattccttgt
     5641 cgcgcgtaat tccagttaca gcaaagcgtt taatgtagta aggtccacct ttcccgtgtc
     5701 ggtaaacggc gttgtaaatc gtacgtttat cgttcttttt gaatacgttg atatacagaa
     5761 tgtttttccc gacaaactgt ttctcggcaa cttttactac cttgtattta ccgtccttaa
     5821 agaaaataat gacatcgtcc atatcggaac agttgcagac gaactcatct tttttcaggg
     5881 ctgttccgat aaatccttca tcgtgattta catacagttt ctcgttagct tccaccactt
     5941 tagcagccac aatagtatcg aaattccgga tttcggtacg ccttggatag tttttgccgt
     6001 atttttcctt cagattttcg aaccagaaaa tggtgtagtc caccagatgc tccaggttat
     6061 attctatttc tttaattttt ttctgaatac caatgatagt atcatttgcc ttgtcggtgt
     6121 cgaatttagt gattcgcacc atctttattt ccagcagttt gaggatatct tcgcgggtta
     6181 cctcacggat aaaatccttt ttgaaaggtt ccagccgact gtcgatatgg gcaaccactt
     6241 cgtcctgatt agaactttct tcaaattccc tgtctttata tattctgttc tctatgaata
     6301 ttttttccag cgaaacaaaa aacagctgat ccagcagttc atcgcgttgt atgataagtt
     6361 cctgctccag caaatcgcgg gtatgatcgg tgctgatgcg taacaattca cttacaccca
     6421 taaaacgggg tttattgtca tgtatcacgc agcagttggg cgaaatactc aactcgcatt
     6481 gagtaaatgc atacagtgca tcaatggttt tatccgatga agttccggga gccaggtgta
     6541 tcagtatttc cacattggcc gaagtattgt catctacctt gcggatttta attttaccct
     6601 tttcgctggc tttgatgatg ctttcaatga gtttggaagt atcggttccg aaaggtattt
     6661 cagtaataac cagcgtttta ttgtcaattt ttccaatttt agaacggatt ttcactgctc
     6721 ctccccgttc accatcgttg taccggctga tgtcaatgcg tcctccggtc tgaaagtcgg
     6781 ggtacaacac gaaatcctca cctttcaggt aagctacgct ggcatcgatg agttcgttga
     6841 agttatgcgg taatatcttg gaattgagcc ccacagcaat accttccacg ccctgagcca
     6901 gtaagagtgg aaattttacg ggcaaattga ccggctcctt gttacgaccg tcgtaggagg
     6961 gtttccattc ggtgattttg ggattaaaaa ccgtatcgag ggcaaatttt gacaggcggg
     7021 cttcgatgta acgcggggct gctgcaccgt cgccggtgag gatgtttccc cagtttccct
     7081 gacagtcgat gagcaggtct ttctgtccca actgcaccag cgcatcaccg atggaggcat
     7141 cgccgtgcgg gtgaaactgc atggtatgcc ccacaacatt ggccaccttg ttgtaacggc
     7201 catcatccag tcgcttcatc gaatgcagaa tacgccgctg caccggtttc agcccatcgt
     7261 aaatatcggg cacagcacgc tccagaatta catacgaagc ataatccaga aaccagttct
     7321 gatacatgcc cgataaatga tgttttacgg catcgcctgt aatttttgga attttatagc
     7381 gggaatgtgt cttgacctct tcgtcctcca tttcattccc ggcgtcctcc atatccgttt
     7441 ccatatccgg tgcttcttca tcggataatt tctgttcttc agcgtcgttg agctcttcgt
     7501 ctttagccat attatttaag cccctatccc cagattgagg aaatattgta aatttaacgg
     7561 ttaatttatt gatagtgtct tctgtcgaca atagttttca aaccacaaag ttaagcaaat
     7621 tttttaaaat tattaagtat ctttggggct gttttcagca ggtaagaaca aaagttatca
     7681 acaattaaaa ggaacttaac atgagattaa aattcattct tccagtcatc atcggcttat
     7741 gttctgtatt tgtttcagct caaacggttg aaaatggcaa gattggcctt acttattcac
     7801 tgaacggagg tacaagtgtt cttcaacctg ccctcgacgg tgcggcaagt cacgagatca
     7861 ccgcagccaa cagcattgga ttgatttatc tgaaacccat caacaattgg ctggaatggg
     7921 aaaccggact gacctacacg ctttacaaag ttaccaccac ctcatcgccc atgcccgaag
     7981 tcactacccg taactcgaca ctatcattaa tcgacattcc catcggcgtc agagccgggt
     8041 tttggaagta tttttttgcc aatggaggct tgctgctcga tctggatatg atgagcagca
     8101 gctcgataag cagtcagtcg ggcgttggac tgatgttggg tctgggactg aaatacgact
     8161 tcagctttgg gggttctctg tttttcaatc cctacggaaa aatccattca ttactcccat
     8221 ttggattgga caaatatcac gaccgcatac tggaaagcgg ctggaaattt ggatttacct
     8281 acaaattgta atgtttcggg tgaaactttc ttgccttaaa acctgctttt aacacctctg
     8341 aaaaaggatt ttaaccaatg aacaaacatc tttcgacagc atcgaaagat attatttgat
     8401 actattggat aatattgttt gacagtatcg gataatattg tatctttgca aaaaaaagta
     8461 ccatgtacag acaagaacaa attgctgaaa ttgctgaaaa ccaaactctt gaatttctga
     8521 acaaagacac cggtataccc cgtgaatttc tcagcagcat tccacaaatt gagaattttg
     8581 ccacgattat tacgggtatt cgtcgttgtg gtaaaagtac gcttttactt caattattta
     8641 aaaatgaaac cgataccgta ttgtatttca attgggaaga cattcgaatt gcaggctttg
     8701 aaacggatga ctttgtccga ttgtacaaag agataataaa gcagaaagtc aacatcctct
     8761 tttttgatga gatacaactt gtcccaaaat gggaaatatt tgttcatcaa cttttgcgtg
     8821 aaggtttccg ggtgtttata accggctcta atgcaacgct gttgagcaaa gaattaggca
     8881 ctcatctcac cggtcgtcat ttatctgtgg agcttttccc gttttcattt tcggaattct
     8941 gccaattcac ccgccaaaat gctgacgatg aagcagtaaa cgattattta cataccggcg
     9001 gaatgcccga atttgtcagg acaagacaat cgtatatcat tcaacaattg gtggaagaca
     9061 tcattacccg cgatatagcc gttagataca acattcgcga tgtagaagcc ttgcggcaac
     9121 tgacagttta tttgatgtca aacgttggaa atatagtgtc ggccaataag ttggccggta
     9181 tgtttggtat taaatcagct tcaactattc tggagtattt ttcacattta actaattcgt
     9241 acttagtcga tttcatacct caatttgatt actcactgaa agcacaaacc cgaaatccaa
     9301 aaaaagtgta tggaattgat accggattgg tttctgaaat ctcgctgtca ttttccagaa
     9361 acacaggaca cattttcgaa aacatgatct acaatcatct gcggatgaag tacaaggaaa
     9421 ttttattttt caataaaaaa ggggaatgtg attttgtaat aaaagaaaaa gaaaaaatca
     9481 ctcaggcaat tcaggtttgc ctgcagctta cagatgaaaa tttccggcgt gaatacgatg
     9541 gtttactcga agcaatgaat tatttcaacc tggatgaagg tttactgatt acaaaaaacg
     9601 aaacagatgt gtttcaaaaa gacggtaaaa caatacgggt ggtaactgcg cggcaatttt
     9661 taagcaactc ctaccatccg gtttaaaaaa tcaataaatt tgagtaaatt tgcaccttga
     9721 ataactaact attaactaaa tctgccttgc tccggcagtc gctgcaattg ggcgaaaaag
     9781 agctttttca ctatggcttc acaagaagat gttttcaaaa aactgatagc tcactgtaag
     9841 gaatacggat ttgtattccc gtcgagcgag atttacgacg gcctgagtgc cgtgtacgat
     9901 tacggtcaaa acggggttga actgaaaaac aacctgaaaa aatactggtg ggacagcatg
     9961 acgctgctgc atgaaaatgt ggtgggaatt gatgctgcca tctttatgca ccccaccacc
    10021 tggaaagctt cgggtcacgt ggatgcattc aatgatccgc ttatcgacaa caaagacagc
    10081 aaaaaacggt atcgcgccga tgtgctgatc gaagatttgc tggctaaata cgacgaaaaa
    10141 attgaaaagg aagtggaaaa agcggccaaa cgttttggcg aaagcttcga cccgaccatg
    10201 tttcgtcaga ccaacccgcg cgtactggaa aatcaggcta aacgcgatga aattcatgcc
    10261 cgctttgtga aagcgctgaa cgacagtaac ctggatgagt tgaaacaaat cattgttgac
    10321 tacgaagtgg tttgccccat aagcggcacc aaaaactgga ccgatgttcg tcagttcaac
    10381 cttatgttct ccaccgaaat gggttcaaca gctgatggag ccatgaaaat ctacctgcgc
    10441 ccggaaacgg ctcagggaat ttttgtaaac tacctgaacg tgcaaaaaac cggccggatg
    10501 aaaattccgt tcggcattgc ccaaatcgga aaagcattta gaaatgagat agttgcccgt
    10561 cagtttattt tccgcatgcg tgaattcgaa cagatggaaa tgcagttctt cgttcgtccg
    10621 ggtgaggaaa tgaagtggtt tgaattctgg aaagagttcc gcctgaaatg gcacaaagcc
    10681 ctcggacttg gcgaccagaa ataccgtttc cacgaccacg ataagctggc tcattatgcc
    10741 aatgccgcta cggatattga atttgaaatg cctttcggtt tcaaggaagt ggaaggaatt
    10801 cactcccgca ccgatttcga cctcggcagc catgagaaat tctccggtaa aaacattaaa
    10861 tatttcgacc cggaactgaa ccagtcatac acaccgtatg tgattgaaac atctatcggc
    10921 gttgaccgta ctttcctgtc gattatggca gccgcctaca ccgaagagga actcgaaggc
    10981 ggcgacagcc gcgtggtgct gaaacttccg cccgtgttgg ctccaatcaa ggcagccatt
    11041 ctgccgttga taaaaaaaga cggactgccc gaaaaagcgc gcgaaataat gaatgaactg
    11101 aaattcgatt tcaaatgtgc ttatgacgaa aaggacagca tcggcaagcg ttaccgccgc
    11161 caggatgcca ttggtacccc attctgtatc accgtagatc atcagacgct ggaagataat
    11221 accgtaacca tccgccaccg cgatgacatg acacaggaac gggtagcgat taccgattta
    11281 caaggtataa tcggcgacta tgtgaatatg aagaatttgt tccggaagat tgcggattaa
    11341 tacaactaca caaaatacac aaatgcctga ttttcaacct cgaaaatcag gcatttacat
    11401 ttatagtata ttgacaaata tcaatatctt agattttctg atttaaacga attacacaaa
    11461 ggattattta tgtgagaaag tcagaaattc cagatttttt agggtaacaa ctgtatcagc
    11521 tcccggctat ggcggatttc aactgtcgag aaaaataata ttaataaccg cttcacaaaa
    11581 aacgtttcac ccttttaaca gttttccact atccacattc tgaaaaacag atcatagacc
    11641 ttatatttcc cgttttcgtt tgtaatataa tccttctcaa gcaggacacg ttgtgccgcc
    11701 tgtacggaac ttgccgatgt cagtttgtat tttcgaataa attcggccga ggtaatggct
    11761 tcagcatctt tggctttagc aatagcaatc aacaattctt tttgtttttc gggcagacga
    11821 aacagaattt cggcataaac ataccggaac gaattaacta cctgatcaac agctgcgggt
    11881 atcatatcca catcgcaggt ttcacccgat tgtgtattcg aaaaaagcat atttaacaat
    11941 ttctgaacat accacgtaat tccttcaaat tgatcgtaaa tttctattac agcctgactg
    12001 ctgattttct ttttattttt ctcaaaatgc ttttgggcaa atgttatata tttacccacc
    12061 ggaattgctt ctaaataaat catcgaaaca ctctgataaa acgggcggga agcacttaaa
    12121 aacatatttc ccatggtgtg gcgctgactt ccggcaaaaa tgaaacaagc attgctgcaa
    12181 tgttgtacat gagtgcgcaa aagcgcttcc acattcgttt cgggataagc ggcaatctgt
    12241 tgaaattcat caatcgctat gatgcatggc tttccggcat tcataaggta ttgaaataac
    12301 tcatccagcg tagctgtttc actcacaata tcgtttaatc ccacattaaa ggaaggtaca
    12361 cccgcaacat caaacgaaat tccggcctgc aaagatttaa ctatggatat aaatccctca
    12421 aaagcctttt gtcccagggg cttcaggcgt tcaataattt ctttgctcaa tgcaagaata
    12481 aactcacgca atgatttggt agcgtaaata tcaatgaaaa atgtaagata atccttcctc
    12541 atctgttcca tgttgaaaac gtgttttact aaacccgttt tgcccattct gcgggtagaa
    12601 atgagggcaa cattattacc gttgatgagt tcgtgaatta aattttctgt ttctgcttcc
    12661 ctgtcgcaga aatactccgg cgaaaggtaa ccggaagtaa tgaatggatt aacaggtgtt
    12721 ttcattgtcg ctacgtttaa cagaattgca tttagcatat tcctttttca ggaaaaggat
    12781 ttatgcaaaa gtaattatta tttttgcata atgcaaaata aataatcaat tttccacata
    12841 cgacatattt ttacagttcc cggggtgcgg tggtttgtaa ccgccgt
//
LOCUS       DAOPZK010000053        12701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_5487_length_12701_cov_23.020797, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000053 DAOPZK010000000
VERSION     DAOPZK010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5487_length_12701_cov_23.020797"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PKH68_04805"
     CDS             <1..860
                     /locus_tag="PKH68_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNX12290.1"
                     /translation="AIETGIPKMRIEEAAARTQARIDSGAQKIIGVNEFRLEKEDPID
                     ILEVDNTAVRIQQIERLKQLRANRNEADVKKALDAITECARTGEGNLLELAVEAARVR
                     ASLGEISDACEAVAGRYKATIRTISGVYSSETKNDSDFKRASELTEKFAKKEGRRPRI
                     MIAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEAAKQAIENDVHVFGVSSLA
                     AGHKTLIPQVMEELKKYGREDIIVIAGGVIPAQDYDFLYKAGVAAIFGPGTSVAKAAC
                     TIMDILLEE"
     gene            1245..1688
                     /locus_tag="PKH68_04810"
     CDS             1245..1688
                     /locus_tag="PKH68_04810"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNX12291.1"
                     /translation="MKKKLTIALVAHDARKADMVEWAIYNASMLSKHKLICTGTTGKL
                     IEEALTKKGVDVDITRKNSGPLGGDAEIAAMVVNKQIDYCVFLIDDLSANPHEADIQM
                     LLRQCRIHNVPIACNRYSADLMITSSLWDDEDYVPSEPKYVKYKR"
     gene            complement(1752..1838)
                     /locus_tag="PKH68_04815"
     tRNA            complement(1752..1838)
                     /locus_tag="PKH68_04815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1802..1804),aa:Ser,seq:gct)
     gene            1962..2990
                     /gene="mltG"
                     /locus_tag="PKH68_04820"
     CDS             1962..2990
                     /gene="mltG"
                     /locus_tag="PKH68_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNX12292.1"
                     /translation="MLLSKSKKVLILLLSVAALLVLSFGFVFLMPNFSPKEGKTTYLL
                     LPDNTAYSEVVDQLEEKMDIFSTFSFNLAAKMLNFSEVKSGRYKISSGMNNFKLIRAL
                     KNGIQTPVQLRINNIRTKEQLAGKLAAQLMTDSVKFLSLLNDTAFLSGINLNPSNSIS
                     VFLPNTYEIFWNTNEKKLFERMNREYEKFWTDTRKQLAAAIPLTPGEVITLASIVDEE
                     TNSKKEKSIVAGLYINRLKKRMPLQADPTIKFAMGDFTIKRVLYVHLQTDSPYNTYKY
                     AGLPPGPIRIPNLETIDAVLNYYKSNYLYMCAKETLNGEHNFAANWAEHQQNAAKYQK
                     ALNERGIK"
     gene            complement(3066..4337)
                     /gene="serS"
                     /locus_tag="PKH68_04825"
     CDS             complement(3066..4337)
                     /gene="serS"
                     /locus_tag="PKH68_04825"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNX12293.1"
                     /translation="MLTLKYITENTDEVIARLKKKHFDGSDIINNIVELDERRKITQT
                     AADNFSAEMNSLSREIGLLFKAGKQVEANEAKEKTITLKENISKLSEQRDEIEQKLQD
                     LLVLIPNLPHQSVPEGKHAEDNITERTGGVFPELGTDAVPHWDLAKKYDLIDFELGVK
                     ISGAGFPVYKGKGARLQRALINFFLDNARDAGYLEVQPPYVVNAASGYGTGQLPDKEG
                     QMYHCNLDDLYLIPTAEVPVTNIYRDVILDEKELPIKNTAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVRIDTPEHSYESLTEMVNYVQSLVEKLELPWRILRLCGGDMSFTSA
                     LTFDFEVYSAAQQRWLEVSSVSNFESYQANRLKCRYRTADKKTQLCHTLNGSALALPR
                     IVAALLENNQTPEGIRIPKALVPYTGFELID"
     gene            complement(4580..4846)
                     /gene="rpmA"
                     /locus_tag="PKH68_04830"
     CDS             complement(4580..4846)
                     /gene="rpmA"
                     /locus_tag="PKH68_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNX12294.1"
                     /translation="MAHKKGQGSSHNGRESESKRLGVKIYGGQVAKAGSIIVRQRGTV
                     HNPGLNIGMGKDHTLFALVAGTVEFRKKKDNKSFVSIKPFEVTN"
     gene            complement(4867..5181)
                     /gene="rplU"
                     /locus_tag="PKH68_04835"
     CDS             complement(4867..5181)
                     /gene="rplU"
                     /locus_tag="PKH68_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNX12295.1"
                     /translation="MYAIVEILGQQFKVEQGKKLFIHRMAEAERGETVEFDKVLLVDN
                     NGTVTVGAPVVNGAKVVCEVISHVRGEKVIVFHKKRRKGYRKRNGHRQDFTELTIKEI
                     IA"
     gene            5552..8074
                     /locus_tag="PKH68_04840"
     CDS             5552..8074
                     /locus_tag="PKH68_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNX12296.1"
                     /translation="MSVKYSEQLNNVFTYSTQEAERLGNAYVGPEHLLLGIFREGNNR
                     AVAVLMEMKVDVGNVKQKIEALVRTDKQPAGGEIPLLKSTEKIKKIMELEARSMSAEA
                     ADTEHLLLAILKDKNNLAVDVLNSEKLTYEKFLAHLSPVNPSDIQMSSNQQSDDEDED
                     EEQFSRKSKSKQTGGKQSETPALDTFGSDITKAAMENRLDPIVGREREIERLAQILSR
                     RKKNNPVLIGDPGVGKSAIVEGLALRITQHKVSRVLFDKRVVALDMASIVAGTKYRGQ
                     FEERIKAILNELQKNPNIILFIDEIHTIVGAGGAPGSLDAANMLKPALARGEIQCVGA
                     TTLDEYRTSIEKDGALERRFQKVMVDPTTPEETLTILQNIQDRYEYHHNVRYTPEAIK
                     ACVRLTDRYITDRCFPDKAIDALDEAGSRVHIATVSVPAEIEQLEKQIEELKNEKNRV
                     IAEQKYEEAVPIRDKEKQCQYQLEDAIKRWEQESQLHPEIVDEEKVAEVVAMMSGIPV
                     QRIAQAEGLKLRQMKDVLKSRVIGQDEAVEKITKAIQRNRIGLKDPGKPIGSFLFLGP
                     TGVGKTHLARVLAEFMFDSPDALIRVDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTE
                     KVRRKPYSIVLLDEIEKAHPDVFNILLQVMDEGRLTDSLGRRIDFKNTIIIMTSNVGT
                     RQLKDFGRGVGFNTGDDAVIDAEFSRGVIQKALNKTFAPEFVNRVDDIVMFDQLNRDS
                     IKSIIDLELKGIFKRVTDLGYKLELSPEAKDFIADKGYDVQYGARPLKRAIQRYLEDE
                     LADVVLSGEAAEGDTILMELNEAKDGVKAKVLKQNPTITETE"
     gene            complement(8234..9100)
                     /locus_tag="PKH68_04845"
     CDS             complement(8234..9100)
                     /locus_tag="PKH68_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNX12297.1"
                     /translation="METKDYLKSFYLKDYSMIFAGLILYALGLTGFLLPNKIVTGGLA
                     GITVLIKSTTEIPLWVSYLTINSALLIIAWKIVGKSFVIKTVVSVGVLTFLLRYGEVY
                     ITRPIIHADPLLSSIIGAMFCGAGLGLVYSVNGSTGGTDIIGAVVTKYRHVSMGRVLL
                     LVDVLIVGSSFFYFQSVEKIAIGLIVMAVMYYAVDLMISGMRQSVQFFIFTSKYEEVA
                     SHINSEIKRGCTIIDGMGWYSKKSQKIIVVLARKTESTSIFRLVKSIDQNAFVTQSNV
                     VGVYGKGFDSLK"
     gene            complement(9157..12030)
                     /locus_tag="PKH68_04850"
     CDS             complement(9157..12030)
                     /locus_tag="PKH68_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNX12298.1"
                     /translation="MEYNFREIEQKWQQYWIDQKTYKTDINTAKQKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLNGFNVLHPMGYDAYGLPAEQYAIQTGQHPAVTTQK
                     NITRYREQLDKIGFCYDWDREVRTCEPDYYKWTQWAFIQMFNSYYSLPPTPSDRGGAR
                     PISELVEHFEKSGTANLNVSCTNELSFTASEWNSKSIKEQQEILLNYRIAYLADMKVN
                     WCPQLGTVLANDEVSEGLSIRGGYPVEQKVMRQWSLRVSAYAQRLLEGLDKIDWTDSL
                     KETQKNWIGRSEGAEMTFRLKDKDYSFDIFTTRADTIYGVTFMVLAPESDLVEICTTP
                     EQSQEVADYIAATKKRTERERIADRRVTGVFSGSYAVNPVSGTEIPIWISDYVLAGYG
                     TGAIMAVPAHDSRDYAFARHFNLPIIPLIEGADISEESFDAKEGIMMNSGFLNGLTVK
                     EAITKAKEYIEEKGIGKVRVNYRLRDAIFSRQRYWGEPFPVYYKEGLPYMIDESKLPL
                     ELPEVDKYLPTETGEPPLGRAKNWIYTPSQTFPKGEGESNYSTSQKVHDTLSSPPSEG
                     IEGRSFPLELNTMPGFAGSSAYYLRYMDPKNDKALVGKEANEYWQDVDLYIGGTEHAT
                     GHLIYSRFWNKFLFDLGIVTKDEPFKKLINQGMIQGRSNFVYRIQGTNTFVSLNLKGQ
                     YDVTPIHVDVNIVSNDILDIEKFKNWNPEYNNAEFILEDGKYICGWAVEKMSKSMFNV
                     VNPDDIVEKYGADTLRLYEMFLGPLEQSKPWDTNGIDGVHRFLKRLWGLYFKDDTLNI
                     SNEAPTAEELKILHKTIKKIGSDIETFSYNTSVSAFMICVNELYTLKCNKKAILEPLT
                     ILLAPFAPHIAEEMYHLLGNTTTVCDAQWPQFNEEYLKESSVNYPVSFNGKVRFTLTL
                     PAEMSREEVEKTALSNEQTQKYLEGKTPKKVIVVPGKIVNIVI"
BASE COUNT         3760 a         2622 c         2648 g         3671 t
ORIGIN      
        1 cagctatcga aaccggaatt ccaaaaatgc gtattgaaga agcggcagcc cgtacccagg
       61 ctcgcattga tagtggagca caaaaaatca tcggtgtgaa cgagtttcgt ctcgaaaaag
      121 aagatccgat tgatattctg gaagtggata atacggcagt acgcattcag cagattgaac
      181 gtctgaaaca attgcgtgcc aaccggaatg aagcggatgt gaaaaaagcg ctggatgcaa
      241 tcactgaatg tgcccgtaca ggagaaggca atcttcttga actggccgtg gaagcagccc
      301 gtgttcgcgc aagtctcggc gaaatctcag atgcctgcga agcagtggca ggtcgatata
      361 aagcaaccat tagaacaatt tcaggcgtgt attcatcaga aaccaaaaat gactcagact
      421 tcaaacgtgc atctgagttg acagaaaaat ttgccaagaa agaaggacgt cgtccccgta
      481 ttatgattgc aaaaatgggt caggacggac acgaccgtgg tgcaaaagtt gtagctaccg
      541 gttacgccga ctgcggcttc gacgttgata tgggaccctt gttccagaca ccggccgaag
      601 cggcaaaaca ggcaattgaa aatgatgttc atgttttcgg agtttcttca cttgctgccg
      661 gtcacaaaac gctgatcccg caggtgatgg aagaattgaa gaaatatgga cgtgaagata
      721 tcatcgtaat tgcaggtggt gttatcccgg ctcaggacta tgacttcctc tacaaagcag
      781 gtgtagcggc tatttttggc ccgggaacct cagtagccaa agcagcttgt acaattatgg
      841 atattttgct ggaagaataa gagcccttct aaatttccct aaaggggaga cttcaaaaaa
      901 aagaaaaacg gacttgcctt atttgaggta agcccgtttt ttttctgcct atttgtccga
      961 tatttcccga attctgacat ttggaacaat aattgtcaga gattgttatt atctaaaagt
     1021 ataatcaaga ttgctaacaa aaaaatgtgc gaacaataaa tgatataaag aagcatttat
     1081 aagaaacacg aacaaaacaa gatgaaacac acatatccaa gtaaaataat tttgacacac
     1141 acgggcatga ttaaaaaact cacaacaaat gatagacgct caatattccc cctttagggg
     1201 gttagggggc aaatatataa attttaaact aaatgaacga aacaatgaaa aagaaactaa
     1261 ctattgcttt ggtggctcac gatgcccgaa aagccgatat ggtggaatgg gcaatataca
     1321 atgcaagtat gctgtcaaaa cacaaactga tatgtaccgg taccaccgga aaattaattg
     1381 aggaagcgtt aactaaaaaa ggcgttgatg tggatattac ccgtaaaaat tcgggcccgc
     1441 tcggcggtga tgccgaaatt gctgcgatgg tggtgaataa gcagattgac tactgtgtat
     1501 ttctgattga tgacctgagt gccaatccgc acgaagctga tattcaaatg ctgttacgtc
     1561 agtgccgcat tcacaatgta ccaattgcct gtaaccgtta cagtgccgac ctaatgataa
     1621 caagttcact ttgggacgat gaggattatg tcccatccga acccaaatat gtgaaatata
     1681 aaaggtaatt gactgattag taataaaata aaaaagaggc agattgtaaa atctgtctct
     1741 tttcattttc gcggaagcgg ggggattcga acccccggta cggttgcccg tacgtcagtt
     1801 tagcaaactg gtggtttcag ccactcaccc acacttccag gttcgtttct gattttgcga
     1861 gtgcaaatat agtgaaagat ttgttagtag caaaaccttt tacccctaaa ataaccaaaa
     1921 cattttcata attttgttcg tttattatca aaacagacat tatgctcctt tcaaaatcaa
     1981 aaaaagtact cattttactt ctatcagtag ctgcattact ggtacttagt ttcggatttg
     2041 tgtttttaat gcccaatttc agtccgaaag aaggtaagac gacatatctc ttattgcctg
     2101 ataataccgc ttacagcgaa gtagtcgatc agttggaaga aaaaatggac atattcagta
     2161 cttttagttt caatctggca gcaaaaatgc ttaatttcag tgaagtaaaa tccggacgat
     2221 ataaaatatc aagtggaatg aataatttca agctgatcag agcgttgaaa aatggtatac
     2281 aaacgcctgt gcagctcaga ataaataata ttcgtaccaa agagcaactt gcgggaaaac
     2341 tggccgctca gctgatgact gactctgtga aatttctgag tttgctcaat gatactgcct
     2401 ttttgtcagg tataaatttg aatccatcca actcaatatc cgtatttttg cccaatacct
     2461 atgaaatatt ctggaatacg aatgaaaaga aactttttga acggatgaac agggagtatg
     2521 aaaaattctg gacagatacc cgcaaacaac tggcagcagc tattccactt acgcccgggg
     2581 aggtcataac acttgcctcg attgtggacg aagaaacgaa cagtaaaaag gaaaaatcaa
     2641 ttgttgccgg actttatatc aacagattaa agaagagaat gcccctgcaa gccgatccga
     2701 ccattaagtt tgcgatgggt gatttcacaa ttaaacgtgt actgtacgtt catctacaga
     2761 ccgattctcc atataatact tacaaatatg cgggattacc tcccgggccc atccgaatac
     2821 ccaatctgga gaccattgat gccgttctca attattataa aagtaattat ttgtatatgt
     2881 gtgccaagga aacactcaac ggtgagcata attttgcggc taattgggcc gagcatcagc
     2941 agaatgctgc aaaatatcag aaagcgctta acgaacgggg aataaaataa attgattttt
     3001 tcaaataaaa aacaggataa tgcattcgtt atcctgtttt ttatttctta aaattcaaat
     3061 gaagtttaat caatcagttc aaagccggtg tatggaacca gtgcttttgg aattctgata
     3121 ccttcaggtg tctgattatt ctcaagtaag gcagccacaa tgcggggcag tgctaatgca
     3181 cttccgttta atgtgtgaca taattgtgtt tttttatctg cagtacggta tcggcatttc
     3241 agccggttgg cctgatagct ttcaaaattc gacaccgaac tgacttccag ccaccgttgt
     3301 tgagcagcgg aatatacttc gaaatcgaag gtcagggcag aagtgaaact catatcgcct
     3361 ccgcaaagcc ggagaattcg ccagggcaat tcaagctttt ccaccaaact ttgaacgtaa
     3421 ttaaccattt cagtaagtga ttcgtaagaa tgttccggag tatcaatacg tacaatttcc
     3481 accttgtcga actgatgcag gcggtttagt ccgcgtacat ccttgccgta tgagcccgct
     3541 tctcttcgga agcaagccga ataagccgta ttttttatcg gaagttcttt ttcatccagt
     3601 attacatcgc ggtaaatgtt ggtaacaggt acttcggcag taggaattaa atacaaatcg
     3661 tccaggttgc agtgatacat ttgtccttct ttgtcgggca gctgaccggt gccgtaaccc
     3721 gatgccgcat tcaccacgta tggtggttgt acttcaagat aaccggcatc acgtgcgttg
     3781 tcaaggaaaa aattaatgag tgcccgttga agacgtgcac ctttaccttt gtagaccggg
     3841 aagccggctc cgctgatttt taccccaagt tcaaagtcaa taagatcata ttttttggcc
     3901 aggtcccaat gtggtacggc atcggtgccg agttcgggaa aaactccgcc ggtacgttct
     3961 gtaatattat cttcggcatg ttttccttcg ggaacacttt gatgaggaag attgggtatg
     4021 agtaccagta aatcctgtag cttttgttca atttcgtccc gttgttcgga taacttgctg
     4081 atattttcct ttaatgttat ggttttttcc tttgcttcgt tggcttcaac ttgtttccct
     4141 gctttgaaaa gcagaccaat ttccctggat aaactattca tctcagcaga gaaattatcg
     4201 gctgcagttt gagtgatttt cctgcgttca tccaactcta caatattgtt gatgatatct
     4261 gaaccatcaa aatgtttttt cttcaaacgg gcaatgactt cgtccgtatt ttctgttatg
     4321 tactttagtg taagcataat attgagccct tctaaattcc cgttattggg actttttgtg
     4381 tgatatgttt gtttttgtta ttctattttt ctaattgata gatcgaactt cctttttttc
     4441 tgtttctaat cggtcaatcg gttttctgta gtcttaagat gcaaagttac aaaaaatgaa
     4501 cagaaaagtg ctattaacaa aaaaatctct cacaaaatat attgcgagag atttttatta
     4561 tctgttaaaa atcagttgtc taatttgtca cctcaaatgg cttaattgaa acaaaagatt
     4621 tgttgtcttt tttcttgcga aattctactg taccagctac gagtgcaaac agtgtatgat
     4681 ctttgcccat accgatgttt aaacccggat tgtgaaccgt accacgctga cgaactataa
     4741 tacttccggc tttagctact tgtccgccat atatttttac tccaagtcgt ttgctttccg
     4801 attcacgacc gttatgtgag ctaccctgac cttttttatg tgccatttta ttttaaattt
     4861 aatcggttaa gctattattt ccttaattgt taattcggtg aagtcctgac ggtgtccgtt
     4921 gcgtttacga taacctttgc gacgtttttt gtggaaaacg attacttttt cacctcttac
     4981 gtgcgaaatc acttcgcata ctacctttgc gccattaacc acgggagctc ctacagttac
     5041 agtgccgttg ttgtcaacca acaatacttt gtcaaattct actgtttcac ctctttcggc
     5101 ttcggccatg cgatgaatga acagcttttt tccttgttca accttgaatt gttgtcccag
     5161 gatttctaca attgcgtaca tttatttttt tattaatgat tacatcgggt aggtgagttg
     5221 tcgctcatga cattgcttct tctattacct tttccgaatt gagggtgcaa atatacggca
     5281 ttttttcaaa acagcaaaat actccccgaa aattatttca agtaaatagt gcataaaagt
     5341 taaataatag atgacggatg aatgtctgcc attttggcag tctgtattaa cacccttatc
     5401 tgccatatta ctgccatctt tgccgaaaaa atgaacaatc tgattttcgg tatgtttttt
     5461 gacattcact acagatattg cttcaatttt tctaaatttg tagcataatt atttatcgaa
     5521 ttgaataact tagtaaaacc aactataata tatgtcagtc aaatattccg aacaattaaa
     5581 taacgtattt acctacagca ctcaagaggc tgaacgactt ggtaatgcgt atgtaggtcc
     5641 agaacatctt ctattgggta ttttcagaga aggtaataac cgagctgttg ccgtgttgat
     5701 ggaaatgaaa gtggatgtag gcaacgtaaa gcaaaaaatt gaagcgctgg tccgtaccga
     5761 caaacaaccg gcaggtggag aaatccccct gctgaaaagt accgaaaaaa ttaaaaaaat
     5821 tatggaactc gaagcccgat cgatgtcagc agaagctgct gataccgaac atttactatt
     5881 ggctattctt aaagataaaa ataaccttgc tgtggatgta cttaactccg aaaagctgac
     5941 ctatgagaaa tttctggctc atctttcacc ggttaatcca tctgacatac agatgagtag
     6001 taaccaacag tctgacgatg aggacgaaga tgaagaacag ttttcaagga aatcaaaatc
     6061 aaagcagacc ggtggaaagc aaagtgaaac tcccgcactg gatacatttg gatcggatat
     6121 aaccaaagca gccatggaaa accgactgga cccaatagtc ggccgtgagc gggaaattga
     6181 acggttagca cagatactca gccgtcgtaa aaagaacaat ccggtgctga ttggcgatcc
     6241 cggagtgggt aaatcggcta tagttgaagg acttgccctg cgtattacac agcacaaagt
     6301 ctcccgggta ttgttcgaca aacgggtagt ggcgctcgat atggcctcta ttgtagcagg
     6361 aaccaagtac cgcggacaat tcgaagaacg tatcaaggca attctgaatg aattgcagaa
     6421 aaatccgaat atcattctct ttattgatga gattcatacc attgtcggtg caggtggagc
     6481 gccaggctca ctggatgctg ccaatatgct caaacctgca ctggcacggg gcgaaattca
     6541 gtgtgtgggt gccactacgc tcgatgaata ccgtacgagc atcgaaaaag acggtgccct
     6601 cgaacgccgc ttccagaaag tgatggttga ccctactaca cccgaagaaa ctctcacaat
     6661 tcttcaaaat attcaggaca ggtacgaata tcatcacaac gtgcgctata cacccgaagc
     6721 catcaaagcc tgcgtacgcc tgaccgaccg ttatatcacc gatcgctgtt tccccgataa
     6781 agccatcgat gccctggatg aagccggctc gcgcgttcat atcgccactg tatcggttcc
     6841 tgcggaaata gaacaactgg aaaagcaaat tgaagaattg aaaaacgaaa aaaacagagt
     6901 aatagccgaa caaaaatacg aagaagcagt tcctattcgt gacaaggaaa aacaatgtca
     6961 gtatcagttg gaagatgcca tcaagcgctg ggaacaagaa tcgcaacttc atcccgaaat
     7021 agtggacgaa gaaaaagtgg ccgaagtggt ggctatgatg tcgggaattc cggtacagcg
     7081 aattgcacag gccgaaggac ttaaacttcg ccagatgaaa gatgtgctga aaagtcgggt
     7141 aataggacag gacgaagctg ttgaaaaaat tacaaaagcc atccagcgaa accggatcgg
     7201 cctgaaagat ccgggaaagc ccatcggttc gttccttttc ttaggcccga caggtgtagg
     7261 gaaaacacac ctggcacgcg tactggccga atttatgttt gatagccctg atgcgctcat
     7321 ccgtgtggat atgagtgagt atatggaaaa attcagcgtt tcgcgactca ttggagctcc
     7381 tccgggatac gtaggttacg aagaaggtgg tcaactgacc gagaaagttc gccgcaaacc
     7441 gtattccatc gtattgctcg atgaaattga aaaagcacat cccgatgttt tcaatatcct
     7501 gctgcaggta atggacgaag gacgattgac cgacagtctc gggcgtcgta ttgatttcaa
     7561 aaatacaatt attataatga cttccaatgt cggtacacgg cagctaaagg actttggacg
     7621 gggagtaggg tttaataccg gtgatgatgc cgttattgat gccgaattct cccggggagt
     7681 tatccaaaaa gcattgaata aaacctttgc acctgagttc gtgaaccgtg ttgatgacat
     7741 tgtgatgttt gatcagctaa accgagacag catcaaatcc atcatcgatc tggagttgaa
     7801 aggtattttc aaacgcgtga ccgacctggg ttacaaactg gaactatccc ccgaagccaa
     7861 ggattttatt gccgataaag gatatgatgt acagtatggt gcccgtcccc tgaaacgtgc
     7921 catccagcgc tacctggaag atgaactggc cgatgtggta ctctccggtg aagcagccga
     7981 aggcgatact attctaatgg aacttaatga agctaaagac ggcgtgaaag cgaaggtgct
     8041 gaaacaaaat ccaacaataa ctgaaacaga atagtaaaat actcttttgt aaaaaaataa
     8101 aaaaaggaac gatttgtgtc gctccttttt tttataagaa tttattctat agaaatcctg
     8161 agaagaaaag aaatttattt ttatttacca aatttgtgtc ttaaagtccc ccctttgggg
     8221 gattaggggg cctttacttc agcgaatcaa accctttgcc gtaaacaccc actacattgg
     8281 attgtgtaac aaatgcattt tgatcgatgc ttttaacaag acggaaaatg gatgtggatt
     8341 cggttttacg ggcaagcacc actatgattt tttgcgattt ctttgaatac cagcccattc
     8401 cgtcaataat agtgcatccg cgcttgattt cagaattgat gtggctggcc acttcctcgt
     8461 atttactggt aaaaataaaa aactgaaccg attgccgcat gccgctaatc atcagatcga
     8521 cagcataata catcacggcc atcacaatca gaccgatagc aattttttcc acactctgaa
     8581 aataaaaaaa tgaagagcca acaatcaata catctaccag caacagcacg cgtcccatac
     8641 tcacatggcg gtatttagtg actaccgcac cgataatgtc ggtgcccccg gtgctgccat
     8701 tgactgaata aaccagccca agtccggctc cgcaaaacat ggcaccaata atggatgaaa
     8761 gcagcggatc ggcatgaata atcggtcggg tgatgtacac ttccccatat cgaagcaaaa
     8821 aagtaagtac acctacactg actactgttt tgataacaaa acttttaccc acaattttcc
     8881 aggcaataat aagtaatgca gaattgatag tcaggtaaga cacccaaagc ggaatttctg
     8941 ttgtagattt gatgagtacg gtgataccag ccaatccgcc ggttacaatt ttgttgggca
     9001 ataaaaaccc cgtcaaaccc agtgcataca ggatcaatcc ggcaaaaatc attgaataat
     9061 ctttcagata aaatgatttt agatagtctt ttgtctccat aataattgaa tttataccaa
     9121 aattaaatca atcataaaaa tcaaaaaatg cattactcag ataacgatat tcacaatttt
     9181 tccgggtact actatcactt tcttcggggt ttttccctcc aggtattttt gtgtttgttc
     9241 gtttgacaga gcagtttttt caacctcttc gcggctcatt tcggccggaa gtgtgagcgt
     9301 aaaacgtact ttcccgttga atgaaaccgg ataatttacc gaactttctt tcaggtattc
     9361 ttcgttgaat tgcggccact gagcatcgca taccgtagtt gtatttccca acagatgata
     9421 catttcttcg gcaatatgag gtgcaaaggg agctaaaaga atggtaaggg gttccagtat
     9481 tgcttttttg ttgcatttca gcgtataaag ttcatttaca caaatcataa atgccgaaac
     9541 tgacgtattg taggagaaag tttcaatatc actgccgatt tttttgatgg ttttgtgcaa
     9601 tatttttaac tcttcggcag ttggagcttc attactaata tttaaagtgt catccttgaa
     9661 atataatccc caaagtcttt tcagaaaacg atgaacgccg tcaattccgt tcgtgtccca
     9721 gggtttggat tgttccagcg gaccgaggaa catttcgtaa aggcggagtg tgtcagctcc
     9781 gtacttttct acaatatcat cgggatttac aacattaaac atggattttg acattttttc
     9841 aaccgcccaa ccgcaaatgt atttgccatc ttccagtata aattcagcat tgttgtattc
     9901 cgggttccag tttttgaatt tctcaatatc cagtatatca tttgaaacaa tatttacatc
     9961 cacgtgaatg ggtgttacgt catattgtcc ttttaaatta agtgaaacaa atgtattggt
    10021 tccctgaatc cggtaaacaa aattgcttct gccctgtatc attccctgat taatcagttt
    10081 tttgaagggc tcatcttttg ttacaatccc caggtcgaac aggaatttat tccagaaacg
    10141 gctgtagatc aggtgtccgg tggcatgttc agtacctccg atatacaaat cgacatcctg
    10201 ccagtactca ttggcctcct tgcccacaag agctttgtcg tttttcggat ccatatatct
    10261 caggtagtaa gcagaagatc cggcaaatcc gggcatggtg ttcagttcga ggggaaaaga
    10321 cctcccctca atcccctccg aaggagggga agagagcgta tcatgtactt tctgcgatgt
    10381 tgagtaattg ctctctcctt cccctttggg gaaggtttgg gatggggtgt agatccagtt
    10441 ttttgcccgt ccaagcggcg gttctccggt ttcggtaggg agatacttgt ccacttcggg
    10501 aagttcgagc ggcagtttgc tctcatcaat catgtagggt aatccttctt tgtaatagac
    10561 cggaaatggt tcaccccaat aacgctgacg actgaaaata gcatcacgca aacgataatt
    10621 taccctgact tttccaattc ctttttcttc gatatattct tttgctttcg taatggcctc
    10681 tttcacggtc agtccgttca gaaaaccgga attcatcatg atgccttctt tggcatcaaa
    10741 actttcttcg ctgatatcgg caccctcaat cagcggaata atcggcagat tgaaatgtcg
    10801 ggcaaacgca tagtcacggc tgtcgtgagc cggaacagcc atgatggccc ccgtgccgta
    10861 tcctgccagt acataatcgc ttatccagat gggaatttcg gtgcccgata caggattgac
    10921 cgcatacgaa ccgctgaaaa ctccggttac acgccggtcg gcaatgcgtt cacgctcggt
    10981 gcgttttttt gtagcagcaa tataatctgc gacttcctga ctttgctcag gcgtggtgca
    11041 tatctccacc aaatcacttt ccggtgccag caccatgaag gttacgccgt agatggtgtc
    11101 tgctcttgtt gtgaaaatat caaatgaata atctttgtct tttaatctga aagtcatctc
    11161 tgcgccctca cttctcccta tccagttttt ctgagtttct ttcagcgaat cggtccagtc
    11221 gatcttgtca agcccttcga gcaaacgctg tgcgtaagca gatacacgta aactccactg
    11281 acgcatcact ttttgctcta ccggataacc gccgcgaatg gaaagtcctt cacttacttc
    11341 atcgttggca agtacagtgc ccaattgcgg acaccagttt actttcatgt cggccaggta
    11401 ggcaatacga taattcaaca ggatttcctg ttgctctttt attgattttg aattccattc
    11461 agaggcggta aaactcaatt cgttggtaca tgacacgttt aaattagctg tgccagattt
    11521 ttcaaaatgc tctactaatt cagaaattgg tcttgctccc cctctatcgg agggggttgg
    11581 ggggaggcta taataagaat taaacatttg gataaatgcc cactgtgtcc atttgtagta
    11641 atcaggttca caggtacgca cttcgcggtc ccagtcgtag cagaaaccga ttttgtcaag
    11701 ctgttcccta taacgggtta tgtttttttg ggtagttact gcgggatgct gaccggtctg
    11761 aatggcatat tgttcggcag gcagaccgta agcatcgtag cccatggggt gaagtacgtt
    11821 gaatccattc agccgtttat agcggctgta aatatcagag gcaatatagc cgaggggatg
    11881 tcccacatgc agtccggcgc ccgatggata tggaaacata tccaacacgt agaatttctg
    11941 ttttgcagta ttgatatccg ttttgtaggt tttttgatct atccagtatt gctgccattt
    12001 ctgctctatt tctctgaaat tgtattccat aataatttga taatgagatg tgtacgaaaa
    12061 tatgtgtttt gccgttataa acgagtgcaa aggtacaaaa aatcatcaga aaaaagcgtc
    12121 ttctttcgta gaatagtttt gcagtgcgat atgtagcagg catgtgtatt tttattcgca
    12181 gaatttgtta attattgaaa ttaatacatt tttgtggttt attttttttt atttaatgag
    12241 ttatattcga tgatttttta tagatttgca ggctttttct cacagatgag ttgtcgctgt
    12301 tattacaatt aggaattaat tcttcaaaaa atagttcaat ttaataagaa ttattccata
    12361 aataatatta tttttgtgga ctgataagga ccgacaatac caaatattgc gtttcgttta
    12421 tatctaaaat taaggaaaaa atgaaaaata attttttcaa ttaataataa atcaaactaa
    12481 attttaaacc cgtgaattta gctcagctga agtcacacaa ttttaaacac attatggcaa
    12541 aacaaattga aactaaaaag aaacagtcaa agggattatc gagtgcgttg gtgattctcc
    12601 ttagctttat cttaggagta cttatttaca aatttgtatt tggttctcca agtaacttta
    12661 ttgacaacaa cccggctaac aatcctcttc agggtaatat g
//
LOCUS       DAOPZK010000054        12691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_5492_length_12691_cov_23.161364, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000054 DAOPZK010000000
VERSION     DAOPZK010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5492_length_12691_cov_23.161364"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..805)
                     /gene="smpB"
                     /locus_tag="PKH68_04855"
     CDS             complement(353..805)
                     /gene="smpB"
                     /locus_tag="PKH68_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNX12299.1"
                     /translation="MSKSNIVIKNKRALFDYEILDRYVAGIQLFGTEIKSIRDGKAGL
                     TDTYCTFIGDELWVRNMNISTYFFGTYNNHEPRRDRKLLLNRKELNKLMRATKETGNT
                     IVPIKLFINDQGRAKIEIGLARGKKTYDKRQTLKEKDDKREMDRAFKR"
     gene            complement(820..1395)
                     /locus_tag="PKH68_04860"
     CDS             complement(820..1395)
                     /locus_tag="PKH68_04860"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNX12300.1"
                     /translation="MKTTDLIIQLFTRSWKLIADTPATWDEIAVEKSTPGSVFLHYTL
                     PLIFICFLFSSFSGYFYADDNRVTIGFLKGLSVALSFLVANLLTYWIGVRALDHFQPN
                     FGVKKNIARLVFYSFCVMYLLRIITSIFPELFFLKVLNIFTAYLVWDGCRGVMGMNED
                     ERSNFVLLVTGMIIFIPLIVRYLLQWMLPII"
     gene            1569..2171
                     /locus_tag="PKH68_04865"
     CDS             1569..2171
                     /locus_tag="PKH68_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNX12301.1"
                     /translation="MTNEIRLHISGMVYNQALGGTYSLVLTEDSGQKRRFSVLIGESE
                     AQSIALKLNNAKSPRPLSHDLMNSIINMLEAKLLKVVIYDMVNDIFYSELYLLHAGKS
                     IVVDARTSDAVALAVRSNAPIYINSDILDIVGTVIEPEADMDDKSEMPASVSLDNLTN
                     DILTRLPEAKLQELLDQAIKEERYEMAAMIRDEMEYRKHH"
     gene            2215..3510
                     /locus_tag="PKH68_04870"
     CDS             2215..3510
                     /locus_tag="PKH68_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX12302.1"
                     /translation="MNLKFRLTIMNFLQFFVWGAWMMTLGHYGFVEKQWNGAEFGLVF
                     STMGFASLVMPTLFGIIADKWKANYVYAILHLLFGTTMCFLPLIDAPVPFFWLLLVAM
                     CFYMPTIGLNNSIGFSVLKKEGKDPTTYFPPIRVWGTVGFIAAMWITNVFTKEWGFGQ
                     SIKVSFIISAIFAFVLSLFSFIFLPVIKTKDENQTASKKTFAQKLGLEAFILFKQKKM
                     ALFFIFSLLLGAALQLTNAYGDSFLQDATVFPKGELINNFSTIILSISQISETLFILA
                     IPFFMKKFGIKKVMLFSMIAWVLRFGLFGIAQNTIPGFILIISSCIIYGMAFDFFNIS
                     GALFVEKNTDAKIQSSAQGLFMLMTNGVGAVIGNIVAGLVIAKWFEHPAVIEGKEMLV
                     KDWPGIWLTFAAYALVVAILFVILFKYKHNPEDIGKISH"
     gene            complement(3580..5154)
                     /gene="dnaB"
                     /locus_tag="PKH68_04875"
     CDS             complement(3580..5154)
                     /gene="dnaB"
                     /locus_tag="PKH68_04875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNX12303.1"
                     /translation="MAEKRNYNKKPVSPAPVAPNEFGKLPPQAPELEEDVLGAIMLEK
                     DAYASIADILKPDFFYKTAHQKIYEAVVDLALHQNPIDMHTVTEMLRKKGTLDEVGGP
                     YYITLLTSRVSSAAHLIYHSQIIVQKYLARELIRMASDVQTKAFDEKMDVDDLMQETE
                     AKLFEITQQNLKKDVVQINPVIEEARKRMKEAANRDGASGIPSGFTELDKITSGWQKS
                     DLIIIAARPAMGKTAFVLSMTKNMAVDFNMPVALFSLEMSNVQLVNRLLMNVCSLEGE
                     KIKNGELTKDEWTKFDRDVTSLYDAPIYVDDTPSLSIFELRSKARRLVKEHNVQCIII
                     DYLQLMNASGMSFGSREQEVSIISRSLKGLAKELDMPIIALSQLNRGLESRATTDKSD
                     GKRPQLSDLRESGAIEQDADMVCFIHRPEYYGMKTDKDGNSTEGIAEIIIAKHRNGAV
                     GDVRLKFKNVYAKFLNLTVDIDPFASQGYATFKSKLNGDEAAPDFYAQPEMRSNPDYL
                     KPAPIGGSNNNNEVPF"
     gene            5341..6156
                     /gene="ispE"
                     /locus_tag="PKH68_04880"
     CDS             5341..6156
                     /gene="ispE"
                     /locus_tag="PKH68_04880"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNX12304.1"
                     /translation="MIVYPNAKINIGLNVVEKRPDGYHNIETIFYPIGLQDVLSVEAS
                     ETCSDYSFAASGIPVGGDAEDNLIVKAYHLLRSGYQFPAVDISLLKQIPFGAGLGGGS
                     ADAAFMLKALNQEFQLKITPKKLEQLAAKLGADCPFFIRNKPVYATGTGNIFTPVNLS
                     LKGYWLLLVKPDIHVSTAEAYSALTPTPSEVPLTELIQKPIVEWKEFIKNDFEKTVFE
                     KQPEIEAIKKEMYRLGALYASMSGSGSSVYGIFGEMPLYEGRFEDCFVVCGEL"
     gene            6238..6582
                     /locus_tag="PKH68_04885"
     CDS             6238..6582
                     /locus_tag="PKH68_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNX12305.1"
                     /translation="MRYFETRFLDEVDVFLAKLDPKTVRKIIYNIDLAEQTNDPKLFK
                     KLQYDIWEFRTIYAGIQIRLLAFWDKTDNKETLVIATHGFVKKVDNVPGNEIKRAVRI
                     KNKYFNNKLAKK"
     gene            6585..6911
                     /locus_tag="PKH68_04890"
     CDS             6585..6911
                     /locus_tag="PKH68_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX12306.1"
                     /translation="MAAKEIKTKSLSDMKDKYIGKPGTQERDEYEYELRMDVLGKMIK
                     TARQERHMTQEELGKLIGVQKAQISKLENSANSATIDTIIKVFKALKAEINFKVKLEN
                     KFVELA"
     gene            7167..7625
                     /locus_tag="PKH68_04895"
     CDS             7167..7625
                     /locus_tag="PKH68_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX12307.1"
                     /translation="MINKDNACCSTKSCCFSLQDYGLLLFRLILGVFMLTHGWAKLSN
                     FSEMSQGFPGMLGMSSSLSLSLIIFAEFFCSIALILGLLTRLAVIPLIIGLGVAAFVA
                     HAGAPFGGRELPLLYFAMYVVLLLTGPGKIALDTLIYNKFCPHGKKNTEG"
     gene            7878..10442
                     /gene="gyrA"
                     /locus_tag="PKH68_04900"
     CDS             7878..10442
                     /gene="gyrA"
                     /locus_tag="PKH68_04900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNX12308.1"
                     /translation="MTDQEDRIIKVNIDDEMKSSYIDYSMSVIVSRALPDVRDGLKPV
                     HRRVLFGMNELGNNSDKPYKKSARIVGEVMGKFHPHGDSSIYGTLVRLAQPWAIRYTL
                     VDGQGNFGSVDGDGAAAMRYTEARLQKIAEEMLADIDKETVDFQSNFDNTLKEPTVLP
                     SRIPNLIVNGSSGIAVGMATNMATHNLSETIDACIAYIENNEIEISELMKYIKAPDFP
                     TGGIIYGYSGVKDAFETGRGRIVIRSKAEIEVGANGHDKIIVNEIPYNVNKSELIKAI
                     ANLVTEKKIEGISNINDETNREGMRIVIDVKRDANASVILNKLFKMTALQSSFSVNNI
                     ALVNGRPRMLNIKQLIKYFIDHRHEVVTRRTQYELGEAEKRAHLLLGFMIILDNLDEA
                     IQIIRSSRTPDEARAALMERFELSEIQSRAVVEMRLRQLTGMEQDKLRAEYEDLLKLI
                     DHLKEILANIELRMQIIKTELEEIKSKYGDKRRSEIVYSSEEFNPEDFYADDEMIITT
                     SNLGYIKRTALSEFRAQGRGGVGSKGSDSREEDFIVNIYPASMHNTMLFFTKKGKCYW
                     LKVYEIPEGTKNSKGRAIQNLLNIDADDAVNAFIRVKGLDDAEYINSHYIIFGTKNGI
                     VKKTLLEEYSRPRQNGVKAITIREDDDVIEVRLTDGNNEIILANRNGRAIRFNESKVR
                     TMGRTATGVKGMTLDNDGMDEVIGMICVHADEPENILVVSEQGYGKRSLLEDYRVTNR
                     GGKGVKTINVTEKTGKLVSIKSVTDENDLMIINKSGITIRIKVADLRVMGRATQGVRI
                     INLEKRNDQIASVCKVNSEEEEEELLNENGIVSEIAETDMDINNEVQNEENNESNQ"
     gene            10519..11739
                     /locus_tag="PKH68_04905"
     CDS             10519..11739
                     /locus_tag="PKH68_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX12309.1"
                     /translation="MKKLILSLCLILGFSAAFAQKSNVAKAENYTLQEKPDFAAAREA
                     IKAAMQDVKTKSDAKTYFTAGMIGFKENEAFNTKMILDAKSVDPLAKGKAIMEAYKYF
                     LIADSLDKLPNAKGKIKPRFGKKIKDNLKELYNGQHNIIAYGAQLFDNKNYKEAYNIF
                     NTYLNMPKLPVMNKELAMDSTYKMIKYYTAIAATNNEDHVNAVSLYTDLKKDNYEPKN
                     VYQLLAEEYRLTKDTDNYLSTLKEGVQKFPKDPWFLQNIINHYIYSDQIPEASKYLDE
                     AIALAPDIAQYYYVKGNVEERLGNNFNARKAFEKALELDPKLAEAYGGIGRLIFNNAV
                     QILNDASMIKDNKLYQKEKDKADNIFRESMPYLKKAVELNPKDTDFKQALKQLYFRLQ
                     MQEEYDAISKELGN"
     gene            complement(12233..>12691)
                     /locus_tag="PKH68_04910"
     CDS             complement(12233..>12691)
                     /locus_tag="PKH68_04910"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNX12310.1"
                     /translation="YETFATGLGLFTSQNVTGAEVWVYNSTYKCAYITGFVSSQNREN
                     EDWLISPQIDLTGKSSPAMCFDHVVRYAGVPANEATVWVSLNYTSGLPSTATWTQIPT
                     NFTNASTWDFYNSGDLSLSAYANKKIRVAFKYTSTATKAGTWEMKNFIVK"
BASE COUNT         3935 a         2585 c         2627 g         3544 t
ORIGIN      
        1 aatgatgtaa aagccggagg agaataaaaa aaataaagca gggaaactga cagatgtcaa
       61 tttccctgct ttattttatc aatttagtat cttaccgggt ttgttatgac ttttctttat
      121 taaaactata ctgtcgctcc gacggagctc atgaatgatt atataattca ttttccacca
      181 taatgtcgca ccgctggtgc tgaaaaacga agcctcgtaa aggcgatatt atggtagaat
      241 ttttatagta ttccaatttt agcctcgtag aggcggcatt atggtagaat tatttttatt
      301 catgtaagat aagttccgaa ggagcgatat aataaaaagt caattcagta tcttatcttt
      361 tgaatgccct gtccatttcc cgtttatcat ccttttcctt cagtgtctgg cgtttgtcgt
      421 acgttttctt cccacgagcc agaccaattt ctattttagc ccttccctgg tcattgataa
      481 aaagtttgat gggaacaatg gtattgccgg tttctttggt tgcccgcatg agtttattca
      541 gttccttgcg gttgagcaga agtttgcggt cgcggcgggg ttcgtggttg ttgtatgttc
      601 cgaaaaaata ggtggaaata ttcatgttcc tcacccaaag ttcatcgcct ataaaagtac
      661 agtacgtatc ggtcagcccg gctttaccat cccggatgga ttttatttcc gttccgaaaa
      721 gctgaatgcc ggccacatac ctgtccagaa tttcataatc gaacagcgcc cgcttatttt
      781 tgattacaat atttgattta ctaatcattg ttgcgtcatt tagattattg gcagcatcca
      841 ctgaagcaga tacctcacta tcaggggtat aaaaataatc atacctgtaa ccagtaacac
      901 gaagttactg cgttcatctt cattcatccc catcacaccg cggcatccat cccataccag
      961 atacgctgtg aaaatattta acaccttcag gaaaaataat tccgggaaga tggatgtaat
     1021 aatccggagt aaatacatca cacagaagga ataaaatact aaccgggcga tatttttctt
     1081 aactccaaaa ttcggttgaa aatgatctaa cgctctgact ccgatccaat aagtaagcag
     1141 attggccaca agaaacgata aagcgacact cagtcctttg agaaatccga tagttacccg
     1201 gttatcatcg gcataaaaat agccagaaaa ggaactgaat aaaaagcaaa taaaaattaa
     1261 cggtaaagta tagtgtagaa aaacacttcc cggagtgctt ttctcaactg ctatctcgtc
     1321 ccaggtagcc ggtgtatcgg ctatcagctt ccatgagcgg gtaaacagct gtattatcag
     1381 gtctgttgtc ttcatctttg aaagtgcaaa attacatata ttttcccgat ttgtgagcta
     1441 ttttttattt atcaacgaat aattcatttc cacattcgta ttttctcaac aaaatcaaat
     1501 tttcaccaaa aattagttat atttgctgtt cgaataaaaa aaagctatca attacaatca
     1561 agggatgaat gacaaacgaa ataagactac acatcagcgg aatggtctat aatcaggcac
     1621 tcgggggaac ttattcattg gttttaaccg aagattcggg acaaaaacga cgcttctcgg
     1681 tacttattgg tgaatcggaa gcacaatcca tagcactcaa gctgaataat gccaaatcgc
     1741 cccgcccgct atcacacgat ctgatgaact ctatcattaa catgctggaa gctaaacttc
     1801 tgaaggtggt gatttacgac atggttaatg atatttttta ttcggaactc tatcttttgc
     1861 atgccggaaa atccatagtg gtagatgccc gcacctccga cgccgtagca cttgccgtac
     1921 gcagcaatgc ccccatttat atcaattccg acatcctgga tattgtcggc acggtgattg
     1981 aacctgaagc agatatggat gataagtcag aaatgcccgc ctctgtgagc ctcgacaacc
     2041 tcaccaacga catactcacc cgactgcccg aagccaaact gcaggaattg ctggatcagg
     2101 caataaagga agagcggtac gaaatggcag ccatgattcg cgatgaaatg gagtaccgaa
     2161 aacatcatta acacgttaga aataataaaa taatacactt aaaaacacaa acgcatgaat
     2221 ttgaaatttc gattgacaat aatgaatttt ctccagttct tcgtgtgggg agcctggatg
     2281 atgacccttg ggcattatgg atttgtagaa aaacaatgga acggagccga atttggcctg
     2341 gtgttctcta ccatgggttt tgcttcgctg gtaatgccta ccctttttgg aattatcgcc
     2401 gacaaatgga aagccaacta tgtatatgcc atactgcatt tgctgtttgg cacaaccatg
     2461 tgttttcttc cgctgattga cgcaccggtg ccatttttct ggttactgct ggtggccatg
     2521 tgtttctaca tgcctaccat cgggctgaac aattccattg gtttcagtgt gttgaaaaag
     2581 gaaggtaaag accccaccac ctatttccca cccatccgcg tatggggaac tgtcggattt
     2641 atagccgcca tgtggattac caatgtgttt accaaagaat ggggcttcgg acaaagcata
     2701 aaagtttcgt tcatcatttc ggctattttc gcattcgtgc tgtcgctgtt ttcatttatt
     2761 tttctacctg taatcaaaac aaaagatgaa aatcagaccg caagcaaaaa aacatttgcc
     2821 caaaaactgg gtcttgaggc atttatcctg ttcaaacaaa agaaaatggc gcttttcttt
     2881 attttcagtt tattactggg agctgccctc cagttgacca atgcttacgg cgacagtttt
     2941 ttgcaggatg ccaccgtgtt tcccaaaggc gaactgatca ataatttctc caccattata
     3001 ctttccatat cccaaatttc ggaaaccctg tttattcttg ccataccttt ctttatgaaa
     3061 aaattcggta tcaagaaggt gatgcttttt agcatgattg cctgggtgtt gcgttttggc
     3121 ctgtttggga tagcacaaaa taccataccc ggatttattt taatcatctc ttcgtgcatc
     3181 atttatggca tggcattcga ctttttcaac atttcgggtg ctttatttgt cgaaaaaaat
     3241 acagatgcaa aaattcaatc atcggcgcag ggacttttta tgctgatgac aaatggagtt
     3301 ggtgccgtaa tcgggaatat agtggccgga ctggtgattg ccaaatggtt tgaacacccg
     3361 gcggtcattg agggtaaaga gatgttggta aaagactggc cgggtatctg gcttactttt
     3421 gccgcttatg cattggttgt tgctattctt tttgttattc tattcaaata caagcacaat
     3481 cccgaagata ttggtaaaat cagccattga tttttagcat ataaattaaa acagccttca
     3541 gtcaaatgac taaaggctgc ttttttttaa ctattattct taaaatggaa cttcgttgtt
     3601 attgttgctt cctccaatgg gagccggttt aagataatcg ggattgcttc tcatttccgg
     3661 ttgagcgtaa aaatcagggg cagcttcatc gccattcagt ttggatttga aggtggcata
     3721 gccctgagaa gcaaaggggt cgatatcgac agtcagattg aggaatttgg catacacatt
     3781 tttgaatttc aggcgcacat cgcccactgc cccgtttcgg tgtttggcta tgataatttc
     3841 ggcaattcct tcggttgaat ttccgtcctt atcggtcttc atgccgtaat attccggacg
     3901 gtgaataaag caaaccatat cggcatcctg ctcaatggca ccggactcac gcaggtccga
     3961 caactgaggt cgtttaccgt ccgatttatc tgtggtggca cgactttcca acccgcggtt
     4021 gagctgcgac agtgcaatga tgggcatatc cagctctttg gcaagtccct tgagcgagcg
     4081 tgaaatgata ctcacctcct gctcgcgact cccaaaggac attccgctgg cattcatcaa
     4141 ctgcaggtag tcaataatga tgcactgcac gttatgttcc ttgaccagtc ggcgggcttt
     4201 actccgaagt tcaaaaatgg acaaactggg cgtgtcgtcc acataaatag gggcatcgta
     4261 aagcgaagtt acatccctgt caaattttgt ccattcatcc ttggtaagtt caccattttt
     4321 gattttttca ccttccaggc tgcacacatt catgagcaaa cgattgacaa gctgcacgtt
     4381 ggacatttca agcgaaaaca acgccacagg catattgaaa tccaccgcca tatttttggt
     4441 catcgaaagc acaaatgccg ttttacccat ggccggacgg gcagcgataa tgataaggtc
     4501 ggatttttgc caacccgaag tgattttgtc cagttcggta aagcctgaag gtattccgct
     4561 cgctccatca cggttggcag cttccttcat acgtttgcgg gcttcctcta tgaccggatt
     4621 gatctgcacc acatcctttt tgaggttttg ctgggtgatt tcaaaaagtt tggcttccgt
     4681 ttcctgcatc agatcatcca catccatttt ttcgtcaaag gctttggtct gtacatcgct
     4741 ggccatacgg atcagttcac gggcgaggta cttctgaaca ataatctgag agtgataaat
     4801 gaggtgtgca gccgatgaaa cccttgaagt aagcagtgtg atgtaatacg gacctcccac
     4861 ttcatccagc gttcctttct tacggagcat ttcggtaacc gtatgcatat caatgggatt
     4921 ttgatgcagg gccaggtcaa ccacagcttc gtagattttc tgatgtgccg ttttgtagaa
     4981 aaaatccggt ttcagtatat cggctatgga ggcgtaagca tccttttcga gcatgattgc
     5041 tcccagcaca tcttcttcga gttcgggggc ctgcggcggt aattttccga actcattagg
     5101 agcaaccggt gcaggggaaa ccggcttttt attgtaattt cttttttctg ccatgaattg
     5161 tattttggaa cttcaaaagt acgaaagaat attcgctgca gcaaataaac ctttcaacaa
     5221 gaaatcaaca atcgtgcagt tatcgctccg ataaacagtt tcggtttctt ttattactcc
     5281 caatgagaat tcctgccaaa attgtatctt tggactttaa aacccataaa tttcaaaatc
     5341 atgatagttt atcccaatgc aaaaataaat atcggtctga atgtggtcga aaagcgtccg
     5401 gacggttatc acaacatcga aaccattttt tatcccatcg ggttgcagga tgtgctgagt
     5461 gtagaagctt ccgaaacgtg cagcgattac agctttgcgg cttcgggcat tcccgtggga
     5521 ggcgatgctg aggataacct gatagtgaaa gcctatcatc tgttacgatc cggctatcag
     5581 tttccggcag tggatatcag tctgttgaaa caaattccct tcggtgcggg gctgggtggc
     5641 ggttcggccg atgctgcctt tatgctcaaa gcattgaacc aggaatttca gctgaaaatc
     5701 actccgaaaa agctggagca attagccgct aaactgggtg ccgactgtcc gttctttatc
     5761 cgcaacaaac ctgtgtatgc caccggaacc ggcaatattt tcacccctgt aaatctctcg
     5821 ctgaaaggtt attggttgtt gctggttaag cccgacattc acgtatcgac ggcagaagca
     5881 tattcagccc tcactcccac tccatcagaa gtaccgctca ccgaactaat ccaaaaaccg
     5941 attgtcgaat ggaaagagtt tattaaaaat gattttgaga aaacggtgtt cgaaaaacag
     6001 ccggaaattg aagctataaa aaaagaaatg taccggttgg gagctttgta tgcttccatg
     6061 tcgggttcag gctcttcggt gtatggcata ttcggtgaaa tgcccctgta tgagggtcgt
     6121 tttgaggatt gttttgtggt ttgtggtgaa ctttaaaaga agaaaaaaga accaataaaa
     6181 gtaaccatat ttggaaactt tatatatctt tgcacaatag aaaaacaaat caaatcaatg
     6241 agatatttcg aaacaagatt tttagatgaa gttgatgtgt ttttggctaa acttgaccct
     6301 aagactgtta gaaaaatcat ttataacatt gatcttgcag aacaaacaaa tgacccaaaa
     6361 ctttttaaga aacttcagta cgacatttgg gaattcagga caatatatgc tggaattcaa
     6421 ataagacttc ttgcattttg ggacaaaaca gacaacaaag aaacattggt aattgcaaca
     6481 catggtttcg taaagaaagt ggacaacgtg ccaggtaacg aaattaaacg agcagtacga
     6541 attaaaaaca agtatttcaa caacaaatta gcaaagaaat aaccatggca gccaaagaaa
     6601 taaagacgaa aagtctttcg gatatgaaag ataaatatat cggaaaaccc ggaacgcaag
     6661 aaagagatga atacgaatat gaactaagaa tggacgtttt aggtaaaatg attaaaactg
     6721 cacgacaaga acgccatatg acacaagagg aattaggaaa attaattggc gtacaaaaag
     6781 ctcaaatatc aaaacttgaa aacagtgcta acagcgcaac tattgacaca ataatcaaag
     6841 ttttcaaagc ccttaaggct gaaataaatt tcaaagttaa acttgaaaat aaatttgttg
     6901 agctcgcttg acgaataagc ttacccccat tacactctaa gccttaccga atatttagta
     6961 tattgaaatt cgggattgat taccgtactt tttttatcac aaaaaaatgc cgggattatt
     7021 ccggaaagat atatgtgcgc ataacagtac gaagtaaaga gaaaaaacgg catttgatat
     7081 agatttccga gactctttcc cgggaattat aaacttttta tcatatcatg cgttgattga
     7141 agtaaacaac aacaaaaaac agatttatga ttaacaaaga caatgcgtgt tgcagcacca
     7201 aaagctgctg tttttcgcta caagattacg gattactatt atttcgcctt attctgggtg
     7261 tatttatgct cacacacggc tgggcaaaac tttctaattt cagtgaaatg agtcagggat
     7321 ttccgggtat gctgggcatg agctcatcac ttagtttgtc actgattatt tttgcagaat
     7381 ttttctgttc cattgctctt attttagggt tacttacccg cctggctgtt attccgctga
     7441 ttatcgggct gggtgttgct gcttttgtgg ctcatgccgg agctccgttc ggcggacggg
     7501 aacttccgct gctctatttt gccatgtacg tggtattatt gcttaccgga cccggtaaaa
     7561 ttgcattgga cacgttgatt tacaataagt tttgtccgca tggaaagaaa aataccgaag
     7621 gttaaaaagg aaaagaagtt ataaccgaac agagaagaat acgatgcaaa ttgtattctt
     7681 ttttgtttta aaatagcttg tgtatttaac aaaaaagtag taatttagtg caaattttca
     7741 gggccgaaac gacggcttct tttttttctt ttaattacac attaatcaac aaataacatc
     7801 ctgaatttca gcgtatttaa ttattatatt ttatttgtgt tgatgaaatt tacaaccgta
     7861 ttaattaata aaatcagatg acagatcaag aagacagaat tattaaagtg aacattgatg
     7921 acgagatgaa atcttcatat atcgactact ccatgtcggt aattgtatca cgcgctttac
     7981 ccgatgttcg cgacggattg aaacccgtac accgccgtgt tttgttcggt atgaacgaac
     8041 ttggcaacaa ctcagacaaa ccctataaaa aatcagccag aattgtgggt gaagtaatgg
     8101 gtaagtttca cccgcacggt gactcatcca tttacggcac actggtacgt ctggcacaac
     8161 cctgggcaat acgttacacc ctcgtcgacg gtcagggtaa cttcggatcg gtggatggtg
     8221 acggcgcagc tgccatgcga tacaccgaag cgaggttgca gaaaatagca gaagaaatgc
     8281 tggctgatat cgacaaagaa actgttgatt ttcagtccaa cttcgataat acgctcaaag
     8341 aaccaaccgt tctcccctcc cgtatcccca acctcattgt gaacggatcg tcgggcattg
     8401 ccgtgggtat ggctaccaat atggccacac acaatctttc agaaacaatt gatgcctgta
     8461 tcgcctatat tgaaaataat gaaatcgaga tttcggagct gatgaaatac atcaaagcac
     8521 ccgatttccc caccggtggt attatttacg gttattcggg agtgaaagat gcttttgaaa
     8581 ccggacgggg cagaattgtt atccgctcaa aagccgaaat tgaagtcggc gccaacggac
     8641 acgataaaat tatagtaaat gaaattcctt ataatgtcaa caaatcggaa ctgattaagg
     8701 ctattgccaa tttggtgacc gaaaagaaaa ttgaaggcat ttcgaacatc aacgatgaga
     8761 ctaatcgtga aggaatgcgt attgttattg acgtcaagcg ggatgcgaat gccagtgtaa
     8821 tactgaataa gttattcaaa atgactgccc tacagtcatc attcagtgta aacaatatcg
     8881 cgctggtaaa tggccgtccg cgcatgctca acatcaagca gctcatcaaa tactttattg
     8941 accaccgaca cgaagtggtt acccgccgca cccaatatga acttggcgaa gctgaaaaac
     9001 gggcacacct gctgcttgga tttatgatta ttctggataa tctggatgaa gctatccaga
     9061 taatccgcag ctcacgaact cccgacgaag cccgtgcggc attgatggaa cgttttgaac
     9121 ttagcgaaat ccagtcgcgt gctgtggttg aaatgcgtct gcgtcagctc accggtatgg
     9181 aacaggataa attgagagcc gaatacgaag atttgctgaa actgattgac cacctgaaag
     9241 aaatcctggc caacatagag ttgcgtatgc aaatcatcaa aactgaattg gaagagataa
     9301 aatccaaata cggagacaaa cgccggagtg aaattgttta ttcatcggaa gaattcaatc
     9361 ccgaagattt ctatgcagac gatgaaatga tcatcaccac atccaacctg ggttatatca
     9421 agcgtaccgc attgagcgaa ttcagagccc agggtcgtgg cggtgtaggt tcaaagggca
     9481 gcgactcgcg cgaggaagat ttcatcgtca acatctatcc cgcttccatg cacaatacca
     9541 tgctgttttt caccaaaaaa ggaaaatgtt actggctgaa agtgtatgaa attcccgaag
     9601 gaacgaaaaa ttcaaaagga agagccattc aaaatctgct caacattgat gcagacgatg
     9661 cagtaaatgc cttcattcgg gtaaaaggac ttgatgatgc tgaatatata aattcacact
     9721 atattatttt tggcacgaaa aatggtattg ttaagaaaac attactggaa gagtattccc
     9781 ggcctcgaca aaatggggtg aaagctatta ccatccgtga ggatgacgat gtgatagaag
     9841 tacgcctgac agatggaaac aacgaaataa tcctggccaa ccgtaatggg cgcgctatcc
     9901 gcttcaacga atcgaaagtg agaacaatgg gtcgcactgc taccggagta aaaggtatga
     9961 cactggacaa tgacggcatg gatgaagtaa ttggcatgat ttgtgttcat gctgatgaac
    10021 cggagaatat tctggtggta tcggaacagg gatacggtaa acgatcgctg ctcgaagact
    10081 acagagtaac caaccggggc ggcaagggcg tgaaaaccat caatgtgacc gaaaaaaccg
    10141 gcaaactggt atctatcaag agcgtaacag acgaaaacga cctgatgata atcaacaaat
    10201 cggggataac aattcgcatc aaggttgccg atctgcgcgt aatgggacga gccacacagg
    10261 gggtacgcat aattaacctt gaaaaacgta atgatcagat agcatccgtt tgcaaggtta
    10321 attccgaaga agaagaggaa gaactcctca acgaaaacgg gatagtatcc gaaatcgctg
    10381 aaaccgatat ggatatcaat aatgaagtgc agaacgaaga gaataacgaa tcaaatcaat
    10441 aaattaataa accaatatgc atctttgatt atttcaaaaa ggcatgatta aacttaacaa
    10501 aaaaacaatt ttacgaacat gaaaaaacta attctatcat tatgtttaat tttgggtttt
    10561 tcagccgcat ttgcacagaa atcaaatgta gccaaagccg aaaactacac gttgcaggaa
    10621 aaacctgatt ttgctgctgc ccgcgaagcg attaaagctg ccatgcagga cgtaaaaaca
    10681 aaatcagatg caaaaaccta cttcacagca ggtatgatcg gattcaaaga aaatgaagca
    10741 ttcaacacaa aaatgatttt ggatgccaaa tctgtagatc cgctggcaaa aggtaaggca
    10801 atcatggagg cttataaata cttcctgata gcagatagtc tggataaact tccaaatgca
    10861 aaaggtaaaa tcaagccacg ttttggaaaa aaaatcaagg ataatctgaa agaattgtac
    10921 aacggacagc acaatatcat cgcttatggg gcacaactgt tcgacaacaa gaactacaag
    10981 gaagcgtata atatatttaa cacctatctg aacatgccca aactgccggt gatgaacaaa
    11041 gagctggcaa tggacagcac ctataaaatg attaagtatt atacagctat tgcagccact
    11101 aataacgaag atcatgttaa cgccgtaagt ctttatactg acctgaaaaa agataattac
    11161 gaaccaaaaa atgtatatca actgcttgct gaagaatacc ggttgacaaa ggatactgat
    11221 aactatctca gcacgctgaa agaaggtgtt cagaaattcc cgaaagatcc atggttcctg
    11281 cagaatatca ttaaccatta catctattcc gatcagatac ccgaagcatc caaatacctg
    11341 gatgaagcaa tcgccctggc tcccgatatt gctcagtatt attatgtaaa gggaaatgtg
    11401 gaagaacggc ttggaaacaa tttcaacgcc cgcaaggcat ttgaaaaagc actggagctt
    11461 gatcctaaac tggcggaagc atatggtggc attggtcgtt tgattttcaa caatgctgta
    11521 cagatactta acgatgccag catgattaaa gacaacaaac tctatcagaa agaaaaagat
    11581 aaggcagata atattttcag agaatcaatg ccttacctga aaaaagctgt agaacttaat
    11641 ccgaaagata ccgacttcaa acaggcattg aaacaacttt atttccgtct gcaaatgcag
    11701 gaggaatatg atgccatttc aaaagaactt ggtaactaat agaatttttc aatctattat
    11761 aagaataaaa gagagtgctt tcgggtactc tcttttattt ttgctcatac tgatgcaaat
    11821 tatcaaagta ttttcaatgc ttacatcaat gtccgcttat tcattgatca aatcacaatc
    11881 ttttagaaat gcttactact caacctgatg ttattctttt tggtcaaggt ccggcattta
    11941 acttttaggc agggcgggat attgtctttg ttttgattaa tttccaccag cacatacaaa
    12001 gaacagaacg aacggaaatg caactttata tatcatttat ctatttttca gggattcgca
    12061 cttattaatt aaaaggaaat aataaggttg cttctgtttc tcaaaaaatc aattctgcaa
    12121 attagcacac atatggacga tatgatcctg cattacacca ttcaaattaa aaaaaatcag
    12181 gctggctgga aaatccagac agcctgattt tgtttggtac atttctgcca gtttatttga
    12241 caatgaagtt tttcatttcc caggttccgg ctttggttgc agtagatgta tatttgaaag
    12301 ctacacgaat tttcttgttg gcataagcac tcagcgaaag atctccacta ttgtagaaat
    12361 cccaggtaga agcattcgta aagttggttg gaatttgtgt ccaggtagca gtagatggca
    12421 atcctgaagt gtaattaagt gatacccata cagtagcttc gttagctgga acgcctgcat
    12481 acctgactac atgatcaaag cacatagccg gtgaagattt gcctgtaagg tcaatctgtg
    12541 gtgaaatcaa ccagtcttcg ttttctctgt tttgagaact tacaaaccct gttatataag
    12601 cacatttgta tgttgaatta tacacccaaa cttctgctcc tgtaacgttt tgtgaagtaa
    12661 acaatcctaa tccggttgca aatgtttcgt a
//
LOCUS       DAOPZK010000055        12391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_5710_length_12391_cov_22.996920, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000055 DAOPZK010000000
VERSION     DAOPZK010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5710_length_12391_cov_22.996920"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1256
                     /locus_tag="PKH68_04915"
     CDS             267..1256
                     /locus_tag="PKH68_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX12311.1"
                     /translation="MKQLEKVIWGVIGAGDVCEVKSVPGMYKTEHSEVKMIMRRNAEK
                     AAGFAHRHGIKHWTTDVNEILNDPDINIIYISTPPDSHCELTLKAAAAGKAVYVEKPM
                     ANTMEECQKMIEACRKAGVPLFVAYYRRALTGFNKLREIIESGEIGKVRFVNIEMYRT
                     PRPYDYNTEGNWRILPGISGGGYFHDMASHQLDYLDSVFGPVVKANGIAVNQAGLYPA
                     DDMVSASLLFESGVVGTGTWCFTTDKSSEKETITILGSAGHLVFNTFGSPMKINITTA
                     EGTKLLEVLHPEHIQQPLIQTIVNELRGTGKCPSTGETGARTTRVMIDITRSI"
     gene            complement(1274..1678)
                     /locus_tag="PKH68_04920"
     CDS             complement(1274..1678)
                     /locus_tag="PKH68_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12312.1"
                     /translation="MNTNSNFERISPRVINKLGENEIFVFGSNLHGQHGGGAARVAYE
                     QFGAVWGQGVGLQGKTYAIPTMHGGIEEIKPYVDEFIEFAQAHPELKFLVTRIGCGIA
                     GFTDEEMAPLFAEASSVTNIFLPESFYNVLSE"
     gene            complement(1788..3236)
                     /locus_tag="PKH68_04925"
     CDS             complement(1788..3236)
                     /locus_tag="PKH68_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNX12313.1"
                     /translation="MQEIKFYNGENIPLELHKVRIVQKLHLVPIERRLEALKEGGFNT
                     FRLSTLDVFLDMLTDSGTNAMSDNQLAAMMKADDAYAGSQSFARLQKAVEDVLGKKYL
                     LPVHQGRAAENILSVAYVRKGSLVPMNYHFTTAMAHITDKGGAIAELLYDEAYVINSD
                     HPFKGNMNIEKLEEIINYHGPKNIPFIRMEASTNLIGGQPFSVQNLRDVRALADKYKI
                     RLVLDCSLLGENAYLVLQREEEFKNSNMETVIKTMTGLADICYFSARKLSSSRGGGIC
                     TDSLEIFRELEAFVPLYEGFLTYGGMSIREVEAMAVGLYETLDETIISQSPTFIKYLV
                     EQLDNKGIPVIKPAGVLGVHVDAMQVCAHIPQTQYPAGALAAALFLISGIRGMERGSI
                     SNQRDEYGNETYADMELVRLAVPRRVFTLSQIKYVEDRMTWLYENRELIGGLKFVYEP
                     PVLRFFMGGLEPVSDWPEKLMAKFKEDFGESL"
     gene            4105..4872
                     /locus_tag="PKH68_04930"
     CDS             4105..4872
                     /locus_tag="PKH68_04930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX12314.1"
                     /translation="MKIKQWYEVLFENYAEKYDNEVFTLGTIGECDFIENEISRDRSL
                     KILDVGCGTGRHSIELSKRGYTVTGIDLSESQLARAREKAKSANLTIDFRKQDARDLP
                     FNAEFDVAIMLCEGGFPLMETDEMNFEILKNVAKSLKIGGKFIFTTLNGLFPLFNSVE
                     DFCASNSEDGNATYKNNTFDLMTFRDFNITEIEDDAGNIRTLECNERYYVPSEITWLL
                     KSLGFTTVEIFGAKLGAFSRSDKLTTVDFEMLVIAQK"
     gene            complement(5428..7881)
                     /gene="priA"
                     /locus_tag="PKH68_04935"
     CDS             complement(5428..7881)
                     /gene="priA"
                     /locus_tag="PKH68_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNX12315.1"
                     /translation="MKYVDVIVPLPLSATFTYIVPDEWSDMVRIGMRVVVPFGTKKMH
                     TAIICLTHSNAPTLYEAKEIICLLDNEPILRFPQLKFWEWMSEYYQAAIGDVYQAAVP
                     AGLKLESETQVRINPDYESDHPLTDKEYKVLDALSDGKIKNVSELNKITEIRNTLPIL
                     KGLLEKGAVEVSEELTEKFRPKTETYIKLTGYARDEENLRKIFDELNRAKKQLEVLMK
                     FIELSRCLSKSPREVSRKELLEQTHALPAIINGLIERGVFENYSKEIGRLDNSEVTTE
                     DMYPLNEFQLQAMNEINRQFTEKPVVLLHGITSSGKTEIYIQLIDKVIASGKQVLYLV
                     PEIALTTQLTSRLKRIFGKRLGVYHSRFSDAERVEIWNNVLHDKSYDVIIGVRSSVFL
                     PFRQLGLVIVDEEHENSYKQYDPAPRYNARNSAIVLASMHGAKTLLGTATPAIETYYN
                     AQTGKYGYVELNQRFQEMELPDILIADMKEAYRKKEMEGHFTPLLLEKITKALNNSEQ
                     VILFQNRRGYAPYLECKACAWVPKCKNCDVSLTVHKFSNKLTCHYCGYTENIPVVCPV
                     CNTPGLVDRGFGTEKIEQEIEEAFPQAKVARMDLDTTRSKKGFEKIISDFEQGKVDIL
                     VGTQMVTKGLDFERVSLVGILNADNLLNFPDFRAHERAFQLMAQVSGRAGRKNKRGTV
                     VLQTSSPEHPVIGQVLRNDYAAMYGTQCTERLQFSYPPFFRLIEITLRHRDSSTLNHA
                     AEDLAVTMRKVFGSRVLGPNIPAVSRIQNWYIKQILLKIETTASAGKAKQILSEITTG
                     LLAKSAYKSTRISIDVDPM"
     gene            complement(7878..8465)
                     /locus_tag="PKH68_04940"
     CDS             complement(7878..8465)
                     /locus_tag="PKH68_04940"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNX12316.1"
                     /translation="MAKVNKISEISDCWWIPGDPLMKEYHDTEWGIPLHDDRKLFEFM
                     VLDAFQAGLSWKTILYRREGFRKAFDNFDMEKIAVYDEQKVQQLMQDASIIRNQAKIR
                     ATITNAQHFISIQKEFGSFDAYIWQFTGGKTLHNHFNTISDIPPRSPQSDAMSKELQK
                     RGFKFVGSTICYAFMQAAGMVNDHLNGCSRNPLKA"
     gene            8604..9140
                     /locus_tag="PKH68_04945"
     CDS             8604..9140
                     /locus_tag="PKH68_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX12317.1"
                     /translation="MNAVKIIEKYYDKNSKLYNILIRHSIKVRDKALKIIDNHPELNA
                     DRQFVKEASMLHDIGIFMTNAPDIECFGTHEYIEHGYLGSEIVRREGYPLHALVCERH
                     TGVGLTLEWIVKNKLPVPYRDMIPLSIEEQIICYADKFYSKTKFDKEHSVEKILKKLS
                     KYDADRAKDFLEWHKRFG"
     gene            9298..9495
                     /locus_tag="PKH68_04950"
     CDS             9298..9495
                     /locus_tag="PKH68_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNX12318.1"
                     /translation="MFTEKIGTSAGLIWSALENGELNVKAVKKATKLSEKDLNLALGW
                     LAREGKVAFREEEAEVFIALV"
     gene            complement(9583..11232)
                     /locus_tag="PKH68_04955"
     CDS             complement(9583..11232)
                     /locus_tag="PKH68_04955"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNX12319.1"
                     /translation="MTKKYLLTALAIVLLSTTVYPCTNFLVGKAATTDGSTFISYSAD
                     SYFLFGALYHYPAATYPKGTMLDINEWDTGKYLGKIKQAEKTYSVIGNINEHQVSIGE
                     TTYGGREELIDTTAVMDYGSLMYIALQRSRTAREAIKVMTDLVAEYGYYSSGESFSIA
                     DPNEVWIMEMISKGPGRKGAVWVALRVPDDCVSAHANQARITTIPFKDKNNCMYSKDV
                     VSFAREKGYFTGKDADFSFSDTYAPLDYSALRICESRVWSFFRKVNPDMDKYISYVKG
                     ETKERMPLWIKPTRKLNAQDLISFMRDHYEGTELDLTKGCAAGPFGSPLRHSPLTFKV
                     DSVEYMHERPTATQQTGFTFVAQMRSWLPDYVGGILWFGVDDAASNVYVPVYCSTDKV
                     PLCFDEKNGSLLRYSPTSAFWTFNAVANFAYGKYSWLIKDIRKLQSELENGFHQMTAS
                     IDTKATSLPEMEARKFLTNYSNDAAEKTTAEWKKLGEYLLVKYMDGNIKKEENGKFIQ
                     NDHAIPPSIIRAGYTPDFQQKIAKENPGLRVKSAAELNNRK"
     gene            11462..11704
                     /locus_tag="PKH68_04960"
     CDS             11462..11704
                     /locus_tag="PKH68_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNX12320.1"
                     /translation="MGIIWFIIIGALAGYLAGKLLKGGGFGLLVNLVVGIIGGVVGGW
                     IFGLLGINASGGLLGSLITSTVGAIVFLWVLSLFKK"
     gene            complement(11774..12280)
                     /locus_tag="PKH68_04965"
     CDS             complement(11774..12280)
                     /locus_tag="PKH68_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX12321.1"
                     /translation="MENFHFEKDLVALQNNMRNFAFSLTLNRDDAEDLLQDTTLKVLD
                     NQDKFTNNVNFKGWVLTIMKNIFINNYRKIVRNQTVFDRTEDLHHLNLPQNSGFDTPE
                     GAFSFGEISNSIASFADEYRVPFSMHVQGYKYEEIAQKMDLPIGTVKSRIFLARKRLQ
                     EQLKDYKY"
BASE COUNT         3426 a         2534 c         2721 g         3710 t
ORIGIN      
        1 tagcaggaat gcccgggaat ttgaaaccgg taaatacttc ttcttttgtt ttcatacgta
       61 aataattgtt tttaattgtc gtatggaact cgtcccgcaa aacgggactc gtttcatttg
      121 attaattatt atgatattaa aatgatatca caaatatagt gtgtgaaaga acagattcca
      181 aataaaacag caaatatttt caaagaattt caatattcct tatcttgcag tctgaaaaag
      241 tcaagacaaa caatatatac aaggttatga aacagctgga aaaagtgatt tggggtgtga
      301 taggtgccgg tgatgtttgc gaagtgaaaa gtgtgccggg aatgtacaaa accgaacact
      361 ccgaagtgaa aatgatcatg cgccgcaatg cggagaaggc cgccggtttt gcgcaccgcc
      421 acggaataaa gcactggact accgatgtga acgagattct gaacgacccc gacattaata
      481 taatctacat atccactcct ccggactcac actgtgagct cacgctaaaa gccgctgctg
      541 caggcaaggc ggtgtatgtg gaaaaaccga tggcaaatac aatggaggag tgtcagaaaa
      601 tgattgaagc ttgccggaaa gccggagtgc ccttgtttgt tgcatactac cgcagagcct
      661 tgaccggctt caacaaatta cgcgaaatca tagaaagcgg agaaatcggg aaagtacggt
      721 ttgtgaatat tgaaatgtac cgcacacccc gaccgtatga ttacaacacg gaaggaaact
      781 ggcgaattct gccgggaatt tccggtggcg gttattttca cgacatggct tcgcaccagc
      841 tcgattatct ggattcagtt ttcggaccgg tggtgaaagc gaacggaatt gctgtcaatc
      901 aggcaggact ttatccggcc gatgatatgg tgtcagcttc cttattgttt gaaagcggtg
      961 tggtgggcac gggtacctgg tgttttacca ccgataaatc ttccgaaaag gaaaccatta
     1021 ccattctcgg ttctgccgga catctggtat tcaatacctt tggcagtccg atgaaaatca
     1081 atataaccac tgccgaagga accaaactgt tggaagtact ccatccggaa catattcaac
     1141 agccgcttat tcagaccatc gtcaatgaat tgcgtggaac aggcaagtgc ccctccaccg
     1201 gtgagacggg agcaagaact acccgtgtga tgattgacat aacccgaagt atttaataag
     1261 tacgggttcg gttttattct gaaagcacat tataaaagct ttcaggcagg aaaatattcg
     1321 ttaccgaact tgcctctgca aataagggag ccatttcttc gtcggtaaag ccggcgatgc
     1381 cgcaaccgat gcgtgtgacc aggaatttga gttcgggatg tgcctgtgca aactctataa
     1441 attcatccac atacggtttt atttcctcaa taccaccgtg catggtggga atggcgtagg
     1501 tctttccctg taatcccaca ccctgacccc agactgctcc gaattgttca taggctaccc
     1561 gcgcggcacc accaccgtgc tgtccatgca ggttacttcc gaagacaaag atttcattct
     1621 cacccaactt gtttattacc cggggcgaaa tgcgttcgaa gtttgaattt gtattcataa
     1681 tcaatttctg taaggttatc aattttcaaa gatatacatt ttcctgaaaa acaacgaaga
     1741 ccttgcaggt cagcaaagtc ttcgttataa agtgaatata aatagaatta aaggctttct
     1801 ccgaaatctt ccttgaattt cgccatcagt ttttcgggcc agtcgctcac cggttccagt
     1861 ccacccataa agaaacgaag taccggtggt tcatatacaa atttcagtcc gccaatcaat
     1921 tccctgtttt cgtacaacca ggtcatgcgg tcttccacgt atttaatttg tgagagggtg
     1981 aacaccctgc gtggaacagc caaacgaacc aattccatgt cggcataggt ttcgttgccg
     2041 tattcatcgc gctgattgga aatagaaccg cgctccatac cacgaattcc tgaaataagg
     2101 aacagagcag cagccagtgc tcctgccgga tattgtgtct gcggaatgtg agcacaaacc
     2161 tgcatggcat ctacgtgaac tccaagtaca ccggcgggtt tgattaccgg aataccttta
     2221 ttgtcgagtt gctccaccag atacttgata aatgtcggac tttggctgat gatggtttcg
     2281 tcgagtgttt cataaagtcc tactgccata gcttccactt cgcggatgga cataccgccg
     2341 taagtgagaa atccttcata aaggggtaca aacgcttcga gctctctgaa aatctcaagg
     2401 ctgtcggtac agatgcctcc accacgtgat gagcttagtt tgcgggctga aaaatagcaa
     2461 atgtcagcca gacctgtcat ggttttgatg actgtctcca tgttggagtt tttgaactct
     2521 tcttcacgct gtagaacaag gtatgcattt tcacccagaa gactacagtc cagaaccaaa
     2581 cgaattttat atttatcggc aagtgcccgc acatctctca gattctgcac agagaaaggc
     2641 tgcccgccta tcaggttggt ggatgcttcc atgcggataa atggtatgtt tttgggcccg
     2701 tggtagttga tgatctcttc cagtttttca atgttcatat ttcccttgaa agggtggtcg
     2761 gagttgatca catatgcttc gtcatacagc aactcggcaa tagctccccc tttgtcggta
     2821 atatgcgcca tagcagtagt aaagtggtaa ttcataggta ccaggctgcc tttgcgaaca
     2881 tatgcaacgg ataaaatatt ctcagcagca cgtccctgat gaacaggcag caggtatttt
     2941 ttacccaata catcttccac agctttttgc aaacgcgcaa aactctgcga tccggcataa
     3001 gcatcgtcgg ctttcatcat ggcagccagt tgattatcgc tcatggcatt ggttccgctg
     3061 tcggtgagca tatccaggaa tacatcgagc gtactcagtc ggaaagtatt gaaacctccc
     3121 tctttcaggg cttccagtcg tcgttcaatg ggtacgaggt gtaatttttg tacgatacgt
     3181 actttgtgta attccagcgg gatattttct ccgttgtaaa atttgatttc ttgcatggtt
     3241 gattttttga ctttttgtca gtattatggt tgattattaa ttgaatttca tgaaaaaaat
     3301 tattgcaaag cagatattgc ctgattgttg atgtctgttt gccaaaaaag tccgtaaatc
     3361 attattttgc ttgcaaaatg tatgaaacga ggcacaaaga tacaaaaata tctatttaac
     3421 gggataaaat tgtattaaat tgtaacgaat tggactaaat tacttacagt ggctcatcgg
     3481 gattacagat agcatcttgt tgttgacgaa gattcatagc aaactcccga aaatatcacc
     3541 ggaaaattta tgaagtttaa cgtaagttcg acatatgaaa tagtccgtta ggacttaaat
     3601 atcaatagaa agaatgtttt ttgttttgat tcatttcccg tagggaattg atatatttat
     3661 cccctaacgg ggaattaaaa ttatttttaa cctatttcta ttgatattat ttccctacgg
     3721 gaatacagac tattgtattt gaaagggttt gttatttata tataacaatt ggcctgaaat
     3781 aattacactg aaaatgatcg gaaaattaca gtttatctgt ctttgtcccg atgatttaac
     3841 atgcatattt acagttgttt ttcttccggt gaataaccct gattaaacaa tcgattttta
     3901 ctctttaacc ccattttata gcaataaata aaccagtttt gtatataaat acaagttact
     3961 tcttgatcaa ttttacgttc tccctacaaa acatttttag agtaagactt catggttaat
     4021 cataaagttt gtaactatta atgtacttga aaatcttttt tctaactttc gaccgaaata
     4081 ttaattccat aaatactatc gataatgaaa atcaaacaat ggtacgaagt gctttttgag
     4141 aactatgccg aaaaatatga taacgaagta tttacgctgg gtacaatcgg tgaatgtgat
     4201 tttatcgaaa acgaaatcag cagagacagg tcattgaaaa tactggatgt aggatgcgga
     4261 acgggacgac attctatcga attgtctaaa cgcggataca ccgtcacagg gattgattta
     4321 tcggaatcgc aacttgcccg cgctcgggaa aaggcaaagt cagctaacct tacaattgat
     4381 ttcagaaaac aggatgcccg ggatttacct ttcaatgccg aatttgatgt ggctatcatg
     4441 ctttgtgaag gcggatttcc gttgatggaa acggacgaaa tgaattttga aatattgaag
     4501 aatgtagcaa aatcattaaa aatcggagga aaatttattt ttacaacctt aaacggacta
     4561 ttcccgctat ttaattctgt ggaagatttt tgcgcatcaa acagtgagga tggaaatgca
     4621 acttacaaaa ataacacgtt tgatttgatg acattcaggg atttcaatat cactgaaatt
     4681 gaagatgacg caggaaatat aagaacgctg gagtgcaatg agcggtatta tgttcccagt
     4741 gaaataacct ggctgcttaa atcgcttgga ttcactactg ttgaaatatt cggagctaag
     4801 ctgggcgctt tttcacgcag cgataaactg accaccgttg attttgaaat gctggttatc
     4861 gctcaaaaat aaagggtaac aatactgaaa actcagcatt cggactttat ctgagtgctg
     4921 agtttgtatt ataaaaaatg attatccata taatatcatg ataatttcaa aacagtttgc
     4981 taacgactga cctcgtccgg tgagccgaaa aatcagtgag aatggcgtta aaaatcgtcc
     5041 ttgtttgaag ttgaagttcg caaaaacata tttctaagta cgaagatttt gggcgaactt
     5101 caacgagttt ggtcccgaac gctttcggga agccagtcga gcgctaattt ttcgagtgaa
     5161 gcgggcgcag tcaaatacag aattcaaact tccctctccg gttggagagg gtgtccgaag
     5221 gacgggtgag gcctttttgc ttactttttt tggtcaagaa aaaaaagtaa gtcgggtttt
     5281 aggggtgaaa cccctattgg ttttatttgt ttactttgcg gataacatat tataaaaacc
     5341 aatatctgaa gataaatcta aaaaagggaa gaaatccaac cggcgcatac ttcaggatag
     5401 atttttgtaa ttgaaggcga aaaatactta catcgggtcc acatcaatgc tgatacgggt
     5461 agatttatag gctgattttg caagcagacc ggtggttatt tcactcagaa tttgctttgc
     5521 ttttcctgct gatgccgtgg tttctatttt cagcagaatt tgtttgatat accaattctg
     5581 aatacgcgac acagcaggga tgttcggccc gagtacccga ctgccaaaaa ctttacgcat
     5641 cgttaccgct aaatcttctg ctgcgtggtt gagtgtggaa ctgtcgcggt ggcgcagggt
     5701 gatttctatc aaccggaaga agggcggata actgaactgt aaccgctcgg tgcactgggt
     5761 gccgtacatg gctgcatagt catttcgcag cacctgaccg ataacgggat gctcaggact
     5821 ggatgtctga agtacaaccg ttccgcgctt gtttttacgg ccggctctgc cgctcacctg
     5881 tgccatgagt tgaaaagccc gttcgtgcgc cctgaagtct ggaaaattga ggagattatc
     5941 ggcattgaga atacccacca gactaacccg ctcaaagtcc agccctttgg tcaccatctg
     6001 cgttccgacc agaatatcca ctttgccctg ttcgaagtcc gaaatgattt tttcaaatcc
     6061 tttcttgcta cgggtagtat ccagatccat acgagccact tttgcctgag gaaatgcttc
     6121 ttctatttcc tgttcaatct tttcggtacc gaaaccacgg tcaaccagtc cgggggtgtt
     6181 gcataccggg cacacgaccg gaatattctc ggtataaccg caatagtgac aggtgagttt
     6241 gttgctgaat ttatgaaccg taaggctcac atcgcagttt ttgcatttcg gcacccaggc
     6301 gcaggcttta cattccaggt aaggtgcata tccgcgccgg ttctgaaaca ggatgacctg
     6361 ttcgctgtta ttcagtgctt tggtgatttt ctccagaagc agaggagtaa aatgaccttc
     6421 catctctttt ttccggtaag cttctttcat atcggctatc aggatgtcgg gcagttccat
     6481 ctcctgaaat cgctggttga gttccacata tccgtatttg cctgtttgag cattgtagta
     6541 tgtttcaatg gcaggtgtgg ctgttccgag caaagttttt gccccgtgca tggaggcaag
     6601 cacgattgct gaattccgcg cattgtagcg gggtgcggga tcgtactgct tgtagctgtt
     6661 ttcgtgttcc tcgtccacaa tgaccagtcc cagctggcgg aaaggcagaa ataccgacga
     6721 gcgaacgccg ataatgacat cgtaactttt atcgtgaagt acattattcc agatttcaac
     6781 ccgttcggca tccgaaaacc gggaatgata cacgcccaac cgcttgccga atatcctttt
     6841 cagacggctg gtaagctggg tggtgagtgc tatttcgggt acgaggtaca acacctgttt
     6901 tcccgatgca attactttgt cgatgagttg aatgtaaatc tcggtttttc cgctggaggt
     6961 aattccatgc agcagcacca caggtttttc ggtaaactgc cggttgattt cattcatcgc
     7021 ctgcagctga aattcattga gcgggtacat atcttcggtg gtcacttccg aattatccaa
     7081 ccggccaatt tctttgctgt aattttcaaa aacgccgcgt tcaatcagtc cgttgatgat
     7141 agccggtaaa gcatgcgttt gttcaagcag ctcctttcgc gatacttccc ggggagattt
     7201 ggataaacaa cgcgacagtt cgataaactt catgagtacc tccagttgct ttttggcgcg
     7261 gttcagttca tcaaatattt ttcgcagatt ttcttcgtcg cgggcgtatc ctgtgagttt
     7321 aatgtaagtt tcagtttttg gtctgaattt ttcggtcagt tcttcactca cttctacagc
     7381 ccctttttct aacagcccct tcagtatggg aagtgtgttg cgtatttcgg taattttgtt
     7441 cagttccgat acattcttta tctttccgtc tgatagggcg tccagcactt tgtactcctt
     7501 gtcggttagt gggtggtcgg attcgtaatc gggattgatc ctcacctgcg tttcgctttc
     7561 gagtttcaat ccggccggaa cagcagcctg atacacatcg ccgatggcag cctgatagta
     7621 ttcgctcatc cattcccaga atttcaactg tggaaaccgg agaatgggtt cattgtcgag
     7681 cagacaaatg atttctttgg cttcgtaaag ggtgggagcg ttgctgtgtg tcaggcagat
     7741 aatcgccgtg tgcatttttt ttgtgccgaa aggaacaacc acacgcatgc caatgcgcac
     7801 catgtcagac cactcgtcgg gaacaatgta agtgaaagtc gcagacagcg gcagtggcac
     7861 tatgacatca acgtatttca tgctttcagg gggtttcggc tgcatccgtt gaggtggtcg
     7921 ttgaccatgc cggcagcctg cataaaggcg tagcagatag tggaacccac aaatttaaat
     7981 ccccgttttt gaagctcttt gctcatcgca tccgattggg gtgaacgggg tggaatgtca
     8041 ctgatggtat tgaaatgatt gtggagcgtt tttcccccgg taaattgcca gatataggca
     8101 tcgaaagatc cgaattcttt ttgtatgctg atgaaatgct gtgcattggt gattgtagct
     8161 ctgattttag cctgattacg aataatggaa gcatcttgca taagttgctg cactttctgc
     8221 tcgtcatata cagcaatttt ttccatatca aaattgtcaa aagcctttct gaaaccctca
     8281 cgcctgtaca atattgtttt ccaactgagc cccgcctgaa atgcatccag caccataaat
     8341 tcaaacaatt tacggtcgtc gtgcagcgga ataccccatt ccgtgtcatg atattccttc
     8401 atcagcgggt cacccgggat ccaccagcaa tcagaaattt cgctgatttt atttaccttt
     8461 gccattaaat tatgcttctg tttgcaaaaa tacaaattaa gattgaattt ctgatttaag
     8521 ttttttaatt ccccggaaag ttgttttttg tgaacttcca aagaagtgaa tttgtttgta
     8581 agtaaataaa atatacgttg acaatgaatg cggttaaaat tattgagaaa tactacgata
     8641 agaattcgaa actatacaac atactaatca gacacagtat taaagtgcgc gataaagcgt
     8701 tgaaaattat agataatcat cccgaactca atgcagaccg ccagtttgtg aaggaagctt
     8761 ccatgctgca cgatattggt atatttatga ccaatgcacc cgatattgaa tgttttggta
     8821 cacatgaata catcgaacac ggatacctgg gttccgaaat cgtacgcagg gaaggatatc
     8881 ctcttcatgc cctcgtatgt gagcggcata ccggcgtggg actgacgctg gagtggatag
     8941 tgaaaaataa actcccggtt ccgtaccgcg atatgattcc cctgagtatt gaagagcaga
     9001 tcatctgcta tgccgataag ttttattcca aaacaaaatt tgacaaagaa cacagcgtag
     9061 aaaaaatact gaagaaatta tcaaaatatg atgctgacag agctaaagac tttctggaat
     9121 ggcacaaacg atttggatag tactacggtt ggacagagat attaattgtt aatattaagt
     9181 taaataagtg taaaaaactg attttcggtg tacagaaacg ttaataatat gttttacaac
     9241 acatttttta gtatttttgt gccgtattac aaatccgaat tataaaaaat aacaattatg
     9301 tttacagaaa aaattggaac aagtgcaggt ttgatatggt ctgctttaga aaacggcgaa
     9361 ctgaatgtaa aagccgtaaa gaaagctaca aaactgtcag aaaaagattt gaatctggct
     9421 ttaggctggt tggctcgtga aggcaaagtg gctttcagag aagaagaagc tgaagtattc
     9481 attgctttag tataaggaat tacactttta acagaataaa agcgggaaaa tctgattttc
     9541 ccgcttttct gttttttgcc ccgtaagtct ctgctaattg tattattttc tgttattcag
     9601 ttccgctgcc gattttaccc gtaatccggg attttctttt gcaatttttt gctgaaaatc
     9661 gggcgtgtag ccggcacgaa taatggaagg tggtatggcg tggtcattct gtataaactt
     9721 gccgttttct tcttttttga tattaccgtc catgtatttt acaagcaggt attcgcctaa
     9781 tttcttccat tccgcagttg ttttttcggc agcatcattg ctgtaattcg tcaggaattt
     9841 ccgcgcttcc atctcaggaa gtgaggtggc tttggtatca atacttgctg tcatctgatg
     9901 aaatccgttt tcaagttccg actgcagttt ccggatatcc tttatcagcc aggagtattt
     9961 tccgtaggcg aaatttgcca ctgcattgaa tgtccagaaa gccgaagtgg gtgagtatct
    10021 cagcaaactt ccattctttt catcaaaaca aagtggaact ttgtcggtgc tgcaatatac
    10081 aggtacgtac acgttgctgg cagcgtcgtc cactccgaac cagagaattc cacccacata
    10141 atcgggcaac cagctccgca tctgagccac aaaggtaaag ccggtttgct gggtagccgt
    10201 gggccgctcg tgcatatact ccacactgtc caccttaaaa gtaagcggac tgtggcgcag
    10261 cgggcttccg aaaggacctg cagcacagcc tttggtcagg tcgagttcgg taccttcgta
    10321 gtggtcgcgc ataaaactga tcaagtcctg agcgttgagt ttgcgggtcg gtttaatcca
    10381 cagcggcatc cgttctttgg tttctccttt cacatagctg atatatttgt ccatatcggg
    10441 atttaccttg cggaaaaacg accacacgcg tgattcgcaa atacgcaacg ctgagtagtc
    10501 gaggggtgcg taggtatccg aaaaactgaa atcggcatct tttccggtga aataaccctt
    10561 ttcgcgggcg aagcttacca catctttgga atacatgcag ttatttttgt ctttgaaagg
    10621 aatggtggtg atacgtgcct ggttggcatg agccgataca caatcgtcag gtacacgcaa
    10681 agctacccat acagcacctt ttcgtccggg tcccttgctt atcatttcca taatccacac
    10741 ttcgttgggg tcggcaatgg agaacgattc gccagagctg tagtaaccgt attcagccac
    10801 caggtcggtc atcactttga tagcctcgcg tgccgtacgc gaacgctgaa gtgcaatata
    10861 catcaggctt ccgtaatcca ttacggcagt tgtatcaatc aattcttcac ggcctccgta
    10921 agtggtctca cctatactta cctgatgttc gtttatgttc ccgatgacgg aataggtctt
    10981 ttctgcctgt tttatttttc ccaggtattt tcccgtatcc cattcattta tgtcaagcat
    11041 ggtgcctttg ggataggtag ctgccggata atgatacaaa gcgccgaaca gaaaatagga
    11101 atccgctgag taggagataa acgtggaacc atcggtagta gctgctttgc ccaccagaaa
    11161 gttggtgcag ggataaaccg tggtagaaag taagacgatg gctaatgctg tcaatagata
    11221 ttttttagtc ataaagaggt gtttttatta atgtatgagt ttaatttgtt agaaaatatt
    11281 attctctaaa ttccaacggg gagcaaaaat agtatttatt tttattgtta aaaaattata
    11341 tgacaggatt tgagattaaa taagatattt aattaaaagt gaaaatttaa aatcgaatga
    11401 gtatatttgc cgatacatat atttcaattt tttacaacag tttttttaaa ataatcaact
    11461 tatgggtata atatggttta ttattatcgg cgccttagcc ggttatctgg ccggtaaatt
    11521 gctcaaaggc ggcggatttg gacttcttgt caatctggta gtgggtataa tcggtggagt
    11581 agtgggtggt tggatttttg gcttgttggg gattaatgct tcgggtggtc ttttggggag
    11641 tttaataacc tcaacggtgg gtgccatcgt ctttttgtgg gtgttgtcgc ttttcaaaaa
    11701 gtaagatgca ctatttctga aaattaaaaa agccttcaaa tttaattgaa ggctttttta
    11761 tgtagtattt gtctcaatac ttataatctt tcagttgttc ctgtagcctt ttacgggcca
    11821 ggaaaatgcg gctttttaca gtaccaatgg gtaaatccat cttctgagca atttcctcat
    11881 acttgtaccc ctgtacatgc atggaaaaag ggacacgata ttcatctgca aatgaagcaa
    11941 tgctgttgct gatttctcca aacgagaaag caccttcagg ggtgtcaaaa ccggaattct
    12001 gcggcaaatt caaatgatgt aaatcttctg tcctgtcaaa tacagtttga ttgcgaacga
    12061 tcttacgata attgttgatg aaaatgtttt tcataatcgt tagcacccaa cccttgaaat
    12121 ttacattgtt ggtaaatttg tcctgattgt ccaacacttt taatgtggtg tcttgcagta
    12181 agtcctcggc atcatcacga tttagcgtta gcgaaaaagc gaaattacgc atgttgtttt
    12241 gtaacgctac aaggtctttt tcaaagtgaa aattttccat ttcgttttta atttagtgtg
    12301 tgaataaatt ttttaaaaac gaattctttt tttgaaaaaa gacttcttct tctcagaagt
    12361 agtcatgtga ttgtcagtta tctatactga a
//
LOCUS       DAOPZK010000056        12365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_5734_length_12365_cov_20.401056, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000056 DAOPZK010000000
VERSION     DAOPZK010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5734_length_12365_cov_20.401056"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKH68_04970"
     CDS             <1..486
                     /locus_tag="PKH68_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX12322.1"
                     /translation="IIGKKMDKAAAETAAQVENAKVETIEDVNGLKAVKVTFDSGILF
                     EFNKSVLNEPSKAALAKFADILIKNPTMDIAVLGHTDNVGSLEANKKVSLARAKVVAD
                     YLVANGAARTQMKTVEGRDYSMPVASNETVEGRAQNRRVEVYMYASEKMIEDAQKEAT
                     K"
     gene            complement(561..1784)
                     /locus_tag="PKH68_04975"
     CDS             complement(561..1784)
                     /locus_tag="PKH68_04975"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNX12323.1"
                     /translation="MQKIKVQNPVVELDGDEMTRIIWKYIKEQLILPYLDLDIKYYDL
                     GIENRDATDDRITVEAAEAINRYNVGIKCATITPDEARVEEFKLKQMWKSPNGTIRNI
                     VGGTVFREPIICSNIPRYVQGWTQPIIIGRHAFGDQYKATDKVIKGKGTLKMTFTAEN
                     GETQSWEVYNFNGDGVAMTMYNTDESIYGFARSSFQMALTKRYPLYLSTKNTILKAYD
                     GRFKDIFQEVYENEFRSEFEKAGITYEHRLIDDMVASAMKWNGGFVWACKNYDGDVQS
                     DTVAQGFGSLGLMSSVLVTPDGKTVEAEAAHGTVTRHYRQHQQGKETSTNPIASIFAW
                     TQGLMHRGKLDGNQPLIDFCQKLEQVCIETVESGLMTKDLAILVYGEKLTSENYLNTQ
                     DFLAALKRNLDEKLN"
     gene            complement(1898..3253)
                     /locus_tag="PKH68_04980"
     CDS             complement(1898..3253)
                     /locus_tag="PKH68_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNX12324.1"
                     /translation="MDNKHLIYKLSESIKRTSKIDKDLFTKFGVKRGLRNEDHSGVLA
                     GLTRVGDVVGYEKQEDGKLKAIPGKLMYRGIDVEDLVNGIKKDDRLGFEETAYLLLSG
                     QLPDQEELEAFKNVLTTTMPLDHKSMMSILSLQGKNIMNILARSVLELYTYDEDPDAI
                     TKDNLIRQSINLIAKFPTIIAYAYQVLRHSQQSRSLHIRFPDSKMSVAENFLYMMKGP
                     GRYTPLDVKILDLALILHADHGGGNNSTFSVRVTSSTETDTYSAIAAGIGSLKGPLHG
                     GANLMVNTMLDDLKENIKNWKDQKEVDAYLIKILNKEAFDKTGLIYGIGHPVYTVSDP
                     RAGLLKEMARELAKEKGREKEFAFMELIEERAVYVFMNYKGEGAKQTCINVDYYSGFV
                     YDAIGLPKEVFTPLFAMARIVGWTAHRIEELNFSSKRIIRPAYKNVRERQDFVPMAER
                     G"
     gene            complement(3390..4709)
                     /gene="icd"
                     /locus_tag="PKH68_04985"
     CDS             complement(3390..4709)
                     /gene="icd"
                     /locus_tag="PKH68_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNX12325.1"
                     /translation="MSIKDNDITPQSPPPAGGNLGGIITKQGDKLIVPDNPVIPFIEG
                     DGIGKEIWTSVKKITDKAVEKAYNGKRKINWLEVLAGEKAFNKTGTWLPAETMEAFRH
                     YRVGIKGPLTTPVGEGIRSLNVALRQELDLYVCLRPVRWFKGVATPLLQPEKVHVTIF
                     RENTEDIYAGIEWNAGTPEVEKVLRFLKEEMGVAKIRFPETTSIGVKPVSTEGTERLV
                     RAAILYAIDNKLPSVTLVHKGNIMKFTEGGFKKWGYALAEREFGEQVFTAEKFKKIEN
                     EQGVDAAVKYYDEAISKGKVFIKDVIADAFLQNTLLKPEEYSVIATLNLNGDYISDQL
                     AAMVGGIGIAPGANINYNTGHAIFEATHGTAPDIAGKNKANPCSMLLSAVMMLEYMGW
                     NEAGEQITKALESMFMNGIATSDLARFMENGRALGTTEFSEELIKRL"
     assembly_gap    4729..4828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4856..7108)
                     /locus_tag="PKH68_04990"
     CDS             complement(4856..7108)
                     /locus_tag="PKH68_04990"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNX12326.1"
                     /translation="MVFDIDMIRKVYASLPEKINQAKKTLQRPLTLTEKILFAHLSPG
                     VPVSDYIRATDYIDFAPDRVAMQDATAQMALLQLMNSGRTTVAVPSTVHCDHLIQAHI
                     NAATDLQTANTTNNEVYQFLTDVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMVGTD
                     SHTPNAGGLGMIAIGVGGADAVDVMAGLPWELKMPKIIGVKLTGKLSGWTSSKDVILK
                     LAGILTVKGGTNAIIEYFGEGTETLSATGKATICNMGAEVGATTSIFPFDEKSAAYLE
                     GSGRKAVADEARKIIDCLQADPEIASSPEKYYDRVIEINLSELEPYINGPFTPDAAYP
                     ISEFNQIVKEKGYPQKMEAGLIGSCTNSSYEDLTRAASIVKKAKDDGLTVKAQFIINP
                     GSEQVRYTAERDGVLGEFESAGAVIMANACGPCIGQWKRDDDTPNRPNSIVTSFNRNF
                     AKRNDGNPNTHAFVASPELVAALTIAGDLCFNPLKDTLLNDKGKAVKLAEPSGYEMPP
                     KGYAVEDAGYLAPSADGNKVEISIDPKSTRLQKLAPFPAWDGKDYTELPLLIKAQGKC
                     TTDHISMAGPWLRFRGHLDNISDNMLIGAVNAFNEKTNAVLDTETGEYMAVPALARKY
                     KAAGLGSVVVAEENYGEGSSREHAAMEPRFLNVKAILVKSFARIHETNLKKQGMLALT
                     FADKEDYDKVQENDKISITGLTTFAPGKNLTVILHHSDGTEERFDVRHTYNDQQIEWF
                     KAGSALNGIK"
     gene            complement(7308..9695)
                     /locus_tag="PKH68_04995"
     CDS             complement(7308..9695)
                     /locus_tag="PKH68_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNX12327.1"
                     /translation="MIRKLLVVLMCLTALSANARQVITGSLTDKETQKPVENASVELV
                     LLPDSSTVELANSSSEGLFILYKADTTRTYCLRVKHLVYKTLVMPLAKKKSSINNVGT
                     ITLEPNTFNLKEIVVNGSKVTVTELGDRTVYGIPDGFKKSSSDGLDVLRKVPAVQVDY
                     LNEDITVNGKTNIRIEVDGVTRDKGYLKRLHPTQISKMEIITSPSGKYDADVDAVINV
                     ITNPAMRYGLKGSAYAGYFPFSTRSQMGMMNGSLDYGFEKISYYISANGILQDIGINS
                     TMKRVAGTTNINQHSDIGMKVGMGNVNVGLIYDPDELNDINLNIAYNNMSNRSEVDAW
                     NYNLTNNIRSIFRNQNNSNNKNDGLNTSLFYKHKFKKDTQHGLEAELKYYNSLNNNTT
                     NNFRNVYYNPLDTSEVSSGPWLEELTNTRVRTLSGQTNYTLPFDSVYFFNTGISGNYN
                     HYRTDNTSPLTQAADMDYRDLRLGGYAELSRNFKKGSIKAGSRFETSHITINSAESGN
                     YFSPLPYANATWKLNDNHNFKVAYSRRVIRPSSAQLNPFVSVVDSQTISRGNINLKPA
                     YRDNFQFTYNLKLGIKKVTLNISPQLFYEYKTGLIQNIISRNSQTGVFENVPTNISNG
                     YETGSALSVNSQIGKVLFNSNFRYMFYHIDKYLDQINATNRQSWNWNSFVMFPLPHDF
                     QFMSVFNFSGPVLDGQTETKSSPFYMLGGGKQFKGNHTLRLFFFNPFLGKFFDSKSTI
                     RNNTVYQQSDYYLKSNLSFMLMYAYSFKLGKTIEKEKHNVEQQLQDNIIKMPISL"
     gene            complement(9724..10629)
                     /locus_tag="PKH68_05000"
     CDS             complement(9724..10629)
                     /locus_tag="PKH68_05000"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HNX12328.1"
                     /translation="MKTFIASLVFALLSISLLAQSPVTLKLNLEKGRVYKVKSTSKQA
                     IQQTANGQQYAIDVLSNTVFSFRVLKQENDVMDISFKLDTIASKISSPMFSRETNSTK
                     PGTEPLEKIMNKMSTYNIVAKISTAGKFLQFVNLGKYTDSILFVLDSVPASKRDDARK
                     QAIALLKESAIKSMIEPIFSYLPEKPVNIGDTWETSYFNVSATLSILLLNTHKLEGIE
                     NNLARVSGTSEAESMPSTDPAAKMTQELKGTSTFDSTIDLITGLSIRNKSKSHFEGVT
                     KMMNGTTEMVMPMKIDGDTETVISK"
     gene            11127..11765
                     /locus_tag="PKH68_05005"
     CDS             11127..11765
                     /locus_tag="PKH68_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNX12329.1"
                     /translation="MDQKKPLIITISREFGSGGAYIGQQIARKRNMYYADSEIVAKAA
                     GQFSANLMDVTSQEERVEPLWKSLWKYYGTVPNVYIPSRRIFVPTSQELFAAQSEIIK
                     RIATEHSAVIIGRCGFHVLRDFPNRLDISLHADIQFRIRRIQGLYNITEEEATSIVKQ
                     SDKDRGLYISTFTGMDWTNSTLFDLSVNTGKIGINKSLELILDFIRLHADNA"
     gene            11790..12119
                     /locus_tag="PKH68_05010"
     CDS             11790..12119
                     /locus_tag="PKH68_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12330.1"
                     /translation="MNEEPLYFTKEGFGVYVMDKNTRELLTEERKMVAMFIDTYGLSA
                     LQIGMFLYSDTTDEFLQVCQVCVNFKDKCIGVYLEKPQKSPCNKHTTHDAAKPKTAKP
                     KTVKTKH"
     gene            12145..12354
                     /locus_tag="PKH68_05015"
     CDS             12145..12354
                     /locus_tag="PKH68_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12331.1"
                     /translation="MAKKVSIVEHEKDADYKVYFCDEEHQQKNHELIAGAKLVVNDEG
                     ADVRVIIVEHEQQAKIHILRKNFPT"
BASE COUNT         3197 a         2571 c         2766 g         3731 t
ORIGIN      
        1 attatcggta aaaaaatgga taaagctgct gctgaaactg ctgctcaggt agaaaatgca
       61 aaagttgaaa caattgaaga cgttaacggt ttgaaagctg taaaagttac attcgactcc
      121 ggaattcttt ttgaattcaa taaaagtgtg ctgaatgaac catccaaagc tgctcttgca
      181 aaatttgccg atattttgat caaaaatcct actatggata ttgcagtact tggccatacc
      241 gacaatgtag gctcattgga agctaacaaa aaagtttcac tggcacgtgc aaaagtggtg
      301 gccgattacc tggtggcaaa tggcgcagcc cgcactcaga tgaaaactgt agaaggccgt
      361 gattattcca tgccggtagc aagcaacgaa actgttgaag gtcgtgctca gaatcgccgc
      421 gtagaagtat atatgtacgc cagcgaaaaa atgattgaag acgctcaaaa agaagctacc
      481 aaataagttg attttattat aatacaaaaa atggctgtca gatttctgac agcctctttt
      541 tgttagatac cataagtaaa ttagttcagc ttttcatcca gatttctttt cagtgcagcc
      601 agaaagtctt gcgtgttgag ataattttca gacgttaatt tttcaccata aacaagaatg
      661 gccagatctt ttgtcatcag accgctttct accgtttcga tacagacctg ctccagcttt
      721 tggcagaaat caatcaacgg ctggttgccg tccagtttgc cacgatgcat caaaccctgt
      781 gtccaggcaa agatggatgc tatgggattg gtggaggttt cttttccctg ttgatgctgg
      841 cggtagtggc gggtgacagt gccgtgagca gcttcggctt ccaccgtttt gccatcggga
      901 gttacgagta cggacgacat cagtccaagc gacccaaatc cctgtgcaac tgtgtccgac
      961 tgaacatctc cgtcgtagtt tttacaggcc catacaaatc cgccgttcca tttcatggcc
     1021 gaagccacca tatcgtcaat caaccggtgt tcgtaagtta tgccggcttt ttcaaattca
     1081 ctgcgaaatt cattttcgta cacttcctga aaaatatcct tgaaacgtcc gtcgtaggct
     1141 tttaggatgg tgtttttggt ggaaaggtaa agcggatacc gttttgtcaa tgccatctga
     1201 aaggatgaac gggcaaaacc gtaaatggac tcatcggtgt tgtacatggt cattgccacg
     1261 ccatctccgt tgaagttgta cacttcccag ctttgggttt caccgttttc ggctgtgaaa
     1321 gtcattttca aggttccttt tcctttgatc actttatcgg tagctttgta ctgatcgccg
     1381 aaggcatgac gaccgatgat gatgggttgt gtccagcctt gtacgtagcg tggaatgttg
     1441 gaacaaataa ttggttcccg aaaaacagtt ccgcctacaa tgttacgaat ggtaccgttg
     1501 ggcgatttcc acatctgttt gagtttaaat tcctctacac gtgcctcgtc tggtgtaatg
     1561 gtggcgcatt ttatacccac attgtaccgg ttgatggctt cggcagcttc aacagttatt
     1621 cgatcatccg ttgcatcgcg gttttcaata cccaaatcgt agtatttgat atccagatca
     1681 aggtagggaa gtattaattg ctctttgata tatttccaga taattcgggt catttcatcg
     1741 ccgtcgagct ccacgacggg gttttgtact tttatttttt gcataagaaa tgaaaaatat
     1801 ttttcagaat aaaactttaa tgtgctgtaa aacaacaaac tccggtcagt aattggtttt
     1861 tcaaactgtg accggagatt gctgtgaatg attgatgtta tcctctttca gccatgggta
     1921 caaaatcctg tctttcgcgt acgtttttgt atgcaggacg gataatgcgc ttgctgctga
     1981 aattaagttc ttcgatgcgg tgagcagtcc agcctacaat acgagccata gcaaagagcg
     2041 gtgtaaatac ttcttttggc agaccgatag catcgtacac aaatcctgaa taataatcaa
     2101 cgttgataca tgtttgtttg gctccttcgc ctttataatt cataaacaca tacacagcac
     2161 gttcttcaat cagttccatg aaggcaaatt ccttttcgcg tcccttttct ttggccagtt
     2221 cgcgcgccat ttccttaagc agtccggcac gcggatcgct gacggtgtaa acgggatgtc
     2281 cgataccata aataagtccg gttttgtcaa acgcttcttt attcagaatt ttaatcagat
     2341 aggcatccac ttctttctga tctttccagt ttttgatatt ttctttcaag tcatcgagca
     2401 tggtattcac catcagattg gcaccaccat ggagtggacc tttcaacgag ccaataccgg
     2461 ctgctatggc cgagtaagtg tctgtttcgg tggaactggt tacacgtacc gagaaagtgg
     2521 agttgttacc accaccgtga tcggcatgaa gtataagtgc aaggtcaagt atttttacat
     2581 ccagtggagt atatctgccg ggacccttca tcatgtacag gaagttttcg gctaccgaca
     2641 ttttgctgtc cgggaaacga atgtgaagcg agcggctctg ctgtgaatgt ctcaatacct
     2701 gatatgcata ggcaatgatg gtggggaatt tggcaatcag attgatagac tgacggataa
     2761 ggttgtcctt ggtaatggca tccggatctt cgtcgtaggt gtaaagttcg agtacgctac
     2821 gggcaagaat attcatgata ttttttccct gcaagctcag aatgctcatc atcgatttat
     2881 ggtcgagcgg catggtcgta gtaagcacat ttttgaatgc ttccaattct tcctgatcgg
     2941 gaagctggcc tgaaagcagc aaataggctg tttcttcaaa tcccaaccgg tcgtcttttt
     3001 taattccgtt taccaggtct tcaacatcta taccacggta cataagtttt ccgggaatgg
     3061 ctttcaactt gccatcttcc tgtttttcgt agccaaccac atcgcctacg cgtgttaatc
     3121 cggccaatac tcctgaatgg tcttcattgc gaagtccacg tttcacacca aatttggtga
     3181 aaagatcctt gtcgattttg ctggttcttt tgatcgactc agaaagcttg taaatcaaat
     3241 gcttgttgtc catgaaaata aaatttatat tgttaatttc tcttttgaat gcaaattaca
     3301 ttaataatgt ttgaagtggt gatattgttc tatgatattt aaattattta tccaaaaaaa
     3361 aatattttag catcgttgaa tgaaatcaat catagtcttt ttataagttc ttccgaaaat
     3421 tcagttgtgc ccagtgctct gccattttcc ataaaccggg caagatcgct ggtggcaatg
     3481 ccattcataa acatactttc caatgctttg gttatctgtt cacctgcttc attccatccc
     3541 atatattcca gcatcatgac agctgaaagc agcatggagc atggattagc cttgtttttt
     3601 cccgcaatgt cgggagcagt tccatgcgtt gcttcaaaga tggcgtgacc ggtattgtag
     3661 ttgatattgg ctcccggagc aatgccaatt ccgccaacca tggccgccaa ctgatcggaa
     3721 atatagtcac cgttcagatt gagcgtggct attaccgagt attcttccgg tttcagtaag
     3781 gtgttttgca ggaaagcatc agctatgacg tctttaatga atacttttcc tttgctgata
     3841 gcttcgtcgt agtatttcac ggcagcatcc acaccctgtt cgttctctat ttttttgaat
     3901 ttttcggcag taaatacctg ttctccaaat tcccgttcgg caagtgcata accccatttt
     3961 ttgaaaccgc cttcggtgaa tttcatgata tttcctttgt gtacgagggt aaccgatggt
     4021 aatttattat caattgcata aagtatggct gcacgaacca gacgttcggt tccttctgtc
     4081 gaaacgggtt tcacaccgat ggaagttgtt tcagggaagc ggattttggc cacacccatt
     4141 tcctctttca gaaaacggag cactttttcc acttcgggtg ttccggcgtt ccattctatt
     4201 ccggcataaa tatcttcggt attttcacgg aagatggtga cgtgtacttt ttccggttgg
     4261 agcaggggag tggctacccc cttgaaccag cgtacgggac gcaggcagac gtacaaatcc
     4321 agttcctgac gcagcgctac attcagcgag cgtatgcctt cacctacggg cgtagtgagt
     4381 ggtcctttta ttcccacccg ataatgacgg aaagcctcca tggtttcagc cggaagccag
     4441 gttccggttt tattaaatgc tttctcaccg gcaagtactt ccagccagtt aatttttcgc
     4501 tttccgttat acgctttttc aaccgcttta tctgtgattt tttttaccga agtccatatt
     4561 tcctttccga taccatcacc ttcgataaat ggaataacgg gattatcagg aacaatcagt
     4621 ttgtcgcctt gtttagtgat tatgccccct aaatttccgc cagccggcgg aggactttga
     4681 ggagtgatat cattgtcttt tatgctcatt tatgttttat ctaattttnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ggaggtaagg aggggcttct ttttcttatt
     4861 ttattccatt cagtgcactt cccgctttaa accactcaat ctgctgatca ttgtaggtat
     4921 gtcgaacatc aaaccgttct tccgtaccgt cggaatgatg taaaatcacg gtcaggtttt
     4981 tccccggtgc aaatgttgta agcccggtaa tgcttatttt gtcattttcc tgcactttat
     5041 cataatcttc tttatcggca aaagtgagtg ccagcattcc ctgttttttc aggttggttt
     5101 cgtgtatgcg ggcaaatgat ttaaccagaa tggctttcac gttgaggaag cggggctcca
     5161 tggcagcgtg ttcgcggctt gaaccttctc cgtaattttc ttcggccacc accactgaac
     5221 caagtccggc agccttgtat ttgcgtgcca atgccggaac tgccatatat tcgccggttt
     5281 cagtatccag tacagcattt gttttttcgt tgaaagcatt cacggcacca atcagcatat
     5341 tgtcggaaat attatcgaga tgaccacgga agcgcaacca cggtccggcc atggatatgt
     5401 ggtcggtagt gcattttccc tgtgctttga taagcagcgg aagttccgta taatcttttc
     5461 catcccaggc agggaatgga gcgagttttt gaagccgcgt ggatttcggg tcaatgctga
     5521 tttccacttt gttgccatca gccgaaggtg caagatagcc ggcatcttct actgcgtagc
     5581 ctttcggagg catttcatat ccactgggtt ctgccagttt aaccgctttg cctttgtcat
     5641 tcagcagtgt gtctttcagc ggattgaaac acaaatcgcc ggcaatggtg agtgcagcca
     5701 cgagttcggg agatgcaaca aaggcatgtg tattgggatt gccgtcattg cgcttcgcaa
     5761 agttgcggtt gaacgaagtg acaattgaat tcggacggtt aggggtatca tcatcgcgtt
     5821 tccactgacc aatacaaggt ccgcaggcat tggccataat cacagcccct gcactttcaa
     5881 attcgcccaa aacaccatca cgttcggcag tatagcgtac ctgttcagaa ccgggattga
     5941 taatgaattg tgctttgact gtcagcccgt catcttttgc tttctttaca atggatgccg
     6001 cccgggtgag gtcttcgtac gaggaattgg tacaggaacc aatcaaaccg gcttccattt
     6061 tttggggata tcctttttcc tttacaattt gattaaattc tgaaattggg taggctgcat
     6121 ccggagtaaa aggcccgtta atataaggtt caagttccga aagattgatt tcaatcaccc
     6181 ggtcatagta tttttcgggg ctggaagcaa tttccggatc ggcttgcagg cagtcgatta
     6241 tttttcgggc ttcatccgcc accgcttttc gtccggaacc ttccaggtag gcagccgatt
     6301 tttcgtcgaa ggggaagatg gaagtggtag cacccacttc ggcacccata ttgcaaatgg
     6361 tggctttacc ggtagccgaa agtgtttcag ttccttcgcc aaagtattca atgatggcat
     6421 ttgttccgcc tttaacggtg aggatgccgg ccagtttgag gattacatct ttggacgatg
     6481 tccagcccga aagttttccg gtgagtttta ccccgattat tttaggcatc ttgagttccc
     6541 acggaagtcc ggccattaca tccacagcgt cggcacctcc cacaccaata gcaatcattc
     6601 ccagaccacc ggcattgggt gtgtgcgagt ctgttcctac catcattcca cccgggaaag
     6661 catagttttc gagtaccacc tgatgaataa ttccggctcc cggtttccag aaaccaatgc
     6721 cgtatttatt cgaaacatcg gtcagaaact gatacacttc attattggtt gtgttggcag
     6781 tttgcaggtc ggtagctgca ttgatgtgag cctgaatgag gtgatcgcag tgaacggttg
     6841 aaggtacggc taccgtggtt cgaccggaat tcatcagttg gagtaaagcc atctgagctg
     6901 tggcatcctg catggcaacc cgatcgggtg caaaatcaat gtaatcagta gcgcggatat
     6961 aatcgcttac gggaacaccc ggtgagagat gtgcaaataa tattttttca gtaagggtga
     7021 gtgggcgttg taatgttttt tttgcctgat taattttttc gggaagtgag gcatacactt
     7081 tgcggatcat atcaatgtcg aaaaccatgt cagttgtagt ttattggttg gtgaaatgtt
     7141 aattaataaa actctaacaa cagagttaag gaattgttca aaccagcatt tgtttttacg
     7201 aaaatgtgga tttcaactgc cttaaatggc gataatatga aacaaaaaca gcctgcccga
     7261 aaacagactg ttctaatgtt tttttggaat aatcaattct atgataatca gagggagatt
     7321 ggcattttga tgatattgtc ctgaagctgt tgttctacat tatgtttctc tttttcaatg
     7381 gttttaccca gtttgaaact gtaggcatac atgagcataa acgaaaggtt tgatttcagg
     7441 taataatcac tttgctgata tacggtattg tttcgtatgg tggatttgct gtcgaaaaat
     7501 tttcccagaa acggattaaa gaagaaaagc cgcagagtat gatttccctt aaattgtttg
     7561 ccgccaccca gcatgtagaa gggcgacgat tttgtttcgg tttgcccgtc gagcaccggt
     7621 ccgctgaaat tgaatacgga cataaactga aaatcatgag gcaggggaaa cataacaaat
     7681 gaattccagt tccagctctg ccggttggtg gcattaatct gatcgaggta cttgtcgatg
     7741 tgataaaaca tatacctgaa gttggaattg aacaagactt ttccaatctg cgaattgacc
     7801 gacagcgcac ttccggtttc ataaccgttg gaaatattgg tcggcacatt ttcgaaaaca
     7861 ccggtctggc tgttgcgact gataatattc tgaattaatc cggttttgta ttcgtaaaaa
     7921 agttgtggtg aaatgttgag cgtaactttt ttaataccga gtttcaggtt gtatgtaaac
     7981 tgaaaattat cgcggtaagc cggtttcaga ttgatattac cacgactgat cgtctggcta
     8041 tccactacac tgacaaaagg attgagctgt gcactggacg gacggataac gcggcgcgag
     8101 taagccactt tgaaattgtg attgtcattg agcttccagg tggcattggc gtagggaagt
     8161 ggggaaaagt agtttccact ttcggcagaa ttgatggtaa tgtgtgaggt ttcgaatctc
     8221 gaacccgcct taatgcttcc cttcttaaaa tttctcgaca gttcggcata acctcccagt
     8281 cttaaatcgc ggtaatccat atcagcagcc tgggtcagcg ggctggtgtt gtctgtgcgg
     8341 taatgattat aatttccact gatacccgta ttaaagaaat atacgctgtc gaaaggcagg
     8401 gtgtaattcg tctgaccgct aagggtacga acccgggtgt tggtgagttc ctccagccac
     8461 ggaccggatg aaacctccga tgtatcaagg ggattataat aaacgtttct gaaattattg
     8521 gttgtattgt tattcagact gttgtaatat ttcagttctg cttccagtcc atgctgtgta
     8581 tcctttttga atttgtgctt gtagaacagc gatgtgttaa gtccgtcatt tttgttgttg
     8641 gaattgtttt gatttctgaa aatgctgcgg atattgttcg ttagattgta gttccaggca
     8701 tccacttccg agcggttcga catgttattg taggcaatat tcaggttaat atcgttcagt
     8761 tcgtcggggt cataaatcag tccaacattc acatttccca taccgacttt cataccaata
     8821 tcactatgtt ggttaatatt ggtcgtgccc gccacccttt tcatggtgga attgatgcca
     8881 atatcctgta atattccatt ggcagaaata taataggata ttttttcgaa accgtagtcg
     8941 aggcttccgt tcatcatgcc catttgagaa cgtgtgctga acggaaaata gccggcatac
     9001 gcacttcctt tcagtccgta gcgcatggca ggattggtta ttacgtttat cactgcatcc
     9061 acatcggcat cgtatttacc cgacggactg gtgatgattt ccattttgct gatttgtgtg
     9121 ggatgcagcc gtttcaggta acccttgtcg cgggttactc catccacttc tattctgata
     9181 tttgttttcc cattcaccgt gatatcttcg ttgaggtagt ccacctgcac agccggtact
     9241 ttgcgaagca cgtcgagtcc gtcggacgat gattttttga aaccatcagg gatgccatac
     9301 acggttcggt cgcccagctc ggttaccgtc actttggaac cattcaccac tatttctttg
     9361 aggttgaaag tgttgggttc gagggtaata gtccccacgt tatttataga gcttttcttt
     9421 ttggccagtg gcattaccag tgttttataa accaggtgtt ttacccgcag gcaataggta
     9481 cgcgtggtat cggccttgta gagtataaaa agtccttcgg aactcgaatt agccaactcc
     9541 acggtgctgc tgtcgggcaa tagtaccaat tccacactgg cattttcaac gggtttttgt
     9601 gtctccttgt cggtaaggct gccggtaatt acctgccggg cattcgccga tagtgcagtc
     9661 aggcacatca gaacaactaa taatttgcga atcatttttc agtaaatatt gttttcagac
     9721 ggtttattta ctgattacgg tttctgtgtc gccgtcaatt ttcatgggca tcaccatttc
     9781 agtagtgccg ttcatcattt ttgttacccc ttcgaaatga cttttcgatt tgttacgaat
     9841 cgacaaaccg gtgataagat ctatcgtgct gtcgaaagtc gaagttcctt tcagttcctg
     9901 tgtcattttg gctgccggat cggtcgaagg cattgattct gcttcggatg ttcctgaaac
     9961 ccgtgccagg ttgttttcaa ttccttccag tttgtgtgtg ttcaacaaca ggatggacag
    10021 ggtagccgaa acgttgaagt aagaggtttc ccaggtatcg ccaatattga ccggtttttc
    10081 gggcagatat gaaaatatgg gttcaatcat gcttttgatg gctgattctt tcaggagagc
    10141 gattgcctgc ttgcgggcat cgtcgcgctt ggaagcggga accgaatcga gcacaaagag
    10201 aatgctgtcg gtgtatttgc ccaggttgac aaactgaagg aatttaccgg cggttgatat
    10261 tttggccaca atattatagg tactcatttt attcatgatt ttttcgagcg gttcggtgcc
    10321 cggttttgtc gaattagttt cgcgcgaaaa catgggagaa cttattttgg aggcaatggt
    10381 atcgagtttg aaagagatat ccatgacatc attttcctgt ttgagtaccc ggaaactgaa
    10441 aacagtattg gataatacat cgatagcata ctgctgtccg ttggctgttt gctggatagc
    10501 ttgtttgctg gtgcttttta ctttgtacac cctgcctttt tcgaggttca gtttcagtgt
    10561 taccggtgat tgtgccagga gcgatatgct caggagggcg aaaacgagag aagcaatgaa
    10621 agttttcatt tttgtaaatg gtttaattgt aatgaattga tatatagttg aataattttt
    10681 aaaatgcggc tgtttgccaa aagaataaac acataactct tatctgtctt caatacaatt
    10741 aagcgtaatg aagatgatgt aactgtaagg atcgttgtaa ggggtgattt ttttcggaag
    10801 aaagtaaatt tcagcttcag taatacgtca aattcctgac aatgtgaggt gtattgctct
    10861 ttttctatct ccagaaacaa cacttcagga ggtttatttc tttagatatt aaggaaatat
    10921 atacattttt cacgttgcaa aagaaacgtt taactgaggc caactgttat aattatcagg
    10981 agaaagtcca tctaattcct ttttgtttgg aatgttgacc tgccgtaaaa aaactgatac
    11041 gactaaaata tctgaaatca gctgtttcag ggcgaatgca caaaattaca gtattttgtt
    11101 tgataattaa caaaaagagg taaaaaatgg atcaaaaaaa accgttaatc attaccatca
    11161 gtcgtgaatt tggcagcggt ggagcttaca tcgggcagca aattgcccga aagcgtaata
    11221 tgtattatgc tgatagtgaa attgtagcca aagctgccgg acagttttca gccaacctga
    11281 tggatgtaac atcgcaggaa gagcgggtag aaccactctg gaaatcgcta tggaaatatt
    11341 acggtacagt tccgaatgta tatattccat cgcgacggat ttttgtgccc acttcacagg
    11401 aattgtttgc cgcccagtcc gaaatcatca aacgcatagc aaccgaacat tcagccgtca
    11461 tcatcggtcg ctgtggtttt catgtacttc gcgattttcc caaccggtta gatatcagtc
    11521 tgcatgccga tatacagttc cgcatccgcc ggatacaggg cttgtacaat attaccgagg
    11581 aggaagcaac cagcatcgta aagcaaagtg ataaagaccg ggggctttat atcagcacgt
    11641 ttaccggaat ggactggacc aactcaacac ttttcgacct ctcggtaaat accggaaaaa
    11701 ttgggattaa taaaagtctc gagctaattc tggattttat aagactacat gcagacaatg
    11761 cataaaaaga gaataaaccg aataaattta tgaacgaaga acccctttat tttacaaaag
    11821 aaggttttgg tgtctatgta atggataaaa acacccggga attgctgacg gaagaacgca
    11881 aaatggtggc tatgtttatt gacacctacg gactttcggc actgcagatt ggtatgtttc
    11941 tgtactctga taccaccgac gagttcctgc aggtctgtca ggtgtgtgtc aacttcaaag
    12001 ataaatgtat aggagtttat ctcgaaaaac cccaaaaaag tccctgcaat aagcatacaa
    12061 cgcacgatgc tgcgaaaccg aaaactgcga aaccgaaaac tgtaaagaca aaacactgaa
    12121 tttacactaa aaagagattg ataaatggca aaaaaggttt cgattgttga gcatgagaag
    12181 gatgccgact ataaggtgta tttctgtgat gaggaacatc aacaaaaaaa ccacgaactc
    12241 attgccggtg ctaaactggt tgttaatgac gagggtgccg atgtaagggt aatcatagta
    12301 gagcacgaac agcaggcaaa aattcatatt ttgaggaaaa attttccaac ataaagcaat
    12361 tataa
//
LOCUS       DAOPZK010000057        12239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_5812_length_12239_cov_24.483503, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000057 DAOPZK010000000
VERSION     DAOPZK010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_12239_cov_24.483503"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..2832
                     /gene="polA"
                     /locus_tag="PKH68_05020"
     CDS             1..2832
                     /gene="polA"
                     /locus_tag="PKH68_05020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNX12332.1"
                     /translation="MKKLFLLDAYAIIYRAYYAFIRNPRVNSKGLNTSAIFGFVNTLE
                     DVLKREKPTHIAVAFDPKGNTFRHEAFTDYKAQREKTPEDIRLSVPIIKEIVKAYNVP
                     VLEVPGFEADDVIGTLAKKAEKEGFEVYMLTPDKDYGQLVSDHIFMYRPKHTGGYEVM
                     GPDAVKAKYELTSHEQVVDLLGLMGDSSDNIPGCPGVGEKTAVKLLLEFGSIDNLLNN
                     TDKLKGALKVKIEENREQILFSRYLATIKTDVPIELEEEKLIMEPMNESALRALFDEL
                     EFRTLSAKIWNTTKNNVDKTVSPATPAPEHGQMSLFDTPTSTEPRQEAQRDLFSQSPD
                     VQVIAGMRSINEVPHKYILVDDKVKRAGLITKLFSQKSVCFDTETTGIDVFTADLVGL
                     SFCFTAGEAYYVSLPAGRSECLEVMKEFKAFFQSDQIQKIGQNIKFDLQMLASYGIQL
                     RGNMFDTMIAHYLLQPELRHGMDYLAEVYLKYRTIHYEDLFAGKGKNQQDIRRVDLNR
                     LCDYAAEDADITFQLKQILETELSRNGLEKLFFEMEMPLLRVLAIMEQNGVRIDIEAL
                     RQSSDILTADMKLLEKEIHQLVGNEFNVGSPMQVGEVLFGQLKLDEKAKKTKTGQYST
                     SEDVLEKLRSKHPVVSKILDYRGLKKLLSTYIDALPLLVNAKTGKVHTTYNQTVAATG
                     RLSSTNPNLQNIPVRDELGREIRKAFIPNDGELLLSADYSQIELRIMAHLSGDANMLE
                     AFNSGQDIHAATAAKIYKIPLEEVTSDMRRKAKTANFGIIYGISTFGLSERLGIPRAE
                     AKELIDGYFETYPDIKRYMEEVIQRAKEQGYVETIYGRKRYLPDINSQNSVVRGFAER
                     NAINAPIQGSAADIIKIAMIRIQEQLEKKNLKATMTMQVHDELNFSVPKDELEVVRQL
                     VIGEMQQAIQLKVPLIADCGVGDNWLEAH"
     gene            complement(2911..3165)
                     /gene="rpsT"
                     /locus_tag="PKH68_05025"
     CDS             complement(2911..3165)
                     /gene="rpsT"
                     /locus_tag="PKH68_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNX12333.1"
                     /translation="MANHKSSLKRIRQTEKRKLANRYYAKTARNAVRKLRSTTEKAAA
                     VEMLPKISAMLDKLAKRNVIHKNKAGNLKSKLSIYVNKLA"
     gene            complement(3200..3271)
                     /locus_tag="PKH68_05030"
     tRNA            complement(3200..3271)
                     /locus_tag="PKH68_05030"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3236..3238),aa:Glu,seq:ttc)
     gene            complement(3574..3888)
                     /gene="trxA"
                     /locus_tag="PKH68_05035"
     CDS             complement(3574..3888)
                     /gene="trxA"
                     /locus_tag="PKH68_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX12334.1"
                     /translation="MTLEFTDENIKEIIESGKPVVIDFWAEWCGPCRMVGPLVEELAE
                     EYAGKVEIGKMNVDENIDTPNEYGIRNIPTILFFKDGKLADKQIGATPKATLKAKVES
                     LL"
     gene            complement(3990..7787)
                     /gene="dnaE"
                     /locus_tag="PKH68_05040"
     CDS             complement(3990..7787)
                     /gene="dnaE"
                     /locus_tag="PKH68_05040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNX12335.1"
                     /translation="MGSFVHLHVHSSYSVLDGMSSISGLVDKAVKSGMHALALTDHGN
                     MFGIKEFYNVCKKKNGPLKDKITVLERKLKVPETGEEEKEDIRKEIELTKLKMIKPIF
                     GSEVYVAQRSRYDKEDKGDRSGYHLVLLAKNETGYRNLCRLVSNGWIQGFYYRPRIDH
                     DLLAKYSEGLIATSACLGGEIHKNIEAGDLAAAERAILWYKKVFGDDYYLELQRHKTD
                     KPGADTQTYVKQELQNKELIALARKTNTKLIASNDVHFVEEDHADAHEHLICLSTGKD
                     LDDPTRMRYTKQEWLKTPEQMAEIFADIPECLENTLEIANKVEFFKLESAPMMPVFPI
                     PAEFGTEEDYRQRYDEGTLREEFGEAFDRIGGYDKAIRIKLDADYLTKLTYQGAERRY
                     GANLNDETRERIDFELDVMKKMGFPGYFLIVQDFIQAARDMNISVGPGRGSAAGSVVA
                     YCLKITNIDPMKYDLLFERFLNPDRISLPDIDIDFEDDKRGQIMRWVTEKYGKKRVAN
                     IVTYGTMGTKSAIKDVARVQKLPLSEADRLTKLIPGRFPNDENDKEIKVNLTNCFKYV
                     SELRDARDSNDPTLANTLKYAEMLEGTVRQTGVHACGLIIGADDLINLVPLSTAVDKE
                     TKEDKVITQFEGTVIEDVGLVKMDFLGLKTLSIIKDTLVNIKKSKGIDIDIDNIPIDD
                     KKTYKLFSKGDTVGVFQFESAGMQKYLKELKPTKFEDLIAMNALYRPGPMDYIPQFIR
                     RKHGQEPIEYAFPVMEKRLNDTYGITVYQEQVMLLSRDLAGFTRGQSDELRKAMGKKL
                     KDKMESLHEKFLQGAQKNGYSDVEKLEQIWSDWAKFASYAFNKSHATCYSLVAYQTAY
                     LKAHYEAEFMAANLTRNKDDITEVGKLMDECKSLKMNVLGPDINESDLTFTVNKDGNI
                     RFGLAGIKNVGAGAVEALIKERETNGKFKNNFDFFERVNLNACNKKNIESLVLAGAFD
                     SLTDIRREQFFVLNSKEELVLDAFIKYGNLYQQDVSFNQNSLFGALEDAVQITKPEVP
                     YAPEWSPLVKLNKEREMIGMYLSAHPMDEFEFEIKNICNATTEDIKDLSRYQLGTGLK
                     IGGMVTAFRDGMTKKGNPYGMITLEDYNGSHEFPLFGKNFDDYAKYMIKDLFLYIHAV
                     VQVKGSDYRNQTPDPNVKPEIELKIMNIMPFKEVRKLITRIRIYLSLDKLTNDVAVEL
                     TKIVTENKGDVNVYIEIKDFLTSEKISLFARPHRMKINSTVYQYLRQAEAEDILRYKI
                     EMT"
     gene            8587..10236
                     /locus_tag="PKH68_05045"
     CDS             8587..10236
                     /locus_tag="PKH68_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12336.1"
                     /translation="MMTKRMLLYILTATAALLFAACNRQQTEADWTHLNKLDSLLAVQ
                     PEATADSLKQIHPARLSHFNRGYYQLLKVIALDKTYYNFTSDSLIDATVHILSRHKRA
                     YPRTYARSLLYQGLVRYRMGVTDSTAYEPIKEAADILEVKKINDPLLLYFCYHYLGLI
                     HNENNNPSYSILYYEKAIKKIKQYGDKNFLLMTYFEITWTYLKINKIDAAKQYIDTLI
                     NVKNITDAQKASINQILSVYYDSTNEPLKALEINKHLVTSDYNYTDLSSMLFKISKNY
                     KALNKLDSALHYAIKAEECKPDSDYYLNNLYYKNIGEISEQLKLWQISANAYRKAYLL
                     KEKATLKALDKEIMSLEKKYDLKEAENKALQLTNRLIFTGFFSLTLLILLVTSFVIFR
                     QRGKHAQIKTQLLAQEKINVERNLIEKEFMLPMYQQISQRNAIIKAFLSDLMTNPYLA
                     KNTQILQKISEIYQDSVKTSNINEFLTDEKFTEFTGIECESCQLLNENEKMLLVFAAM
                     KLDNRQIAVLFNTTESSVRGRKAKLRVKIENKNIDIKGIII"
     gene            10424..10807
                     /locus_tag="PKH68_05050"
     CDS             10424..10807
                     /locus_tag="PKH68_05050"
                     /inference="COORDINATES: protein motif:HMM:NF023021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12337.1"
                     /translation="MKNLIFRFILMVFVFGISSVSLHAGDVNYEQEIEFRKQNPAGQP
                     RAPSRYPVSFTGTYFMDELIVGSKNYTGEITVQITGISGSLIQAVQISASEYFTIDLS
                     SLPEGVYDIAIITEGKGTFNGVFEK"
     gene            11082..11792
                     /locus_tag="PKH68_05055"
     CDS             11082..11792
                     /locus_tag="PKH68_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNX12338.1"
                     /translation="MKKFIFILSLTTLLISCNLDTAPGLLMETESIFLFHGETAVLGA
                     SSENGLVYYKSSDSLVAEVDEKGVITANVVGTTYITVLDGKEAKACKVTVTPRFLVPI
                     NPYVNFGASVSEVKKAVKDVFIEELSKINNSSEKTLVYYNQKENEKSRIAYEYRFLND
                     KLISTGVYVNKDFEYFDNMMDYLDETYYYKDEFKTTAASSALSCQRIYQCVEQKVDLK
                     VYYKIPVTEYYLLFSEKN"
BASE COUNT         3565 a         2525 c         2566 g         3583 t
ORIGIN      
        1 atgaaaaaac tcttcttact cgacgcctac gccattattt acagggcata ttatgctttt
       61 atccgcaatc cacgcgtcaa ttcaaaagga ttgaatactt ctgctatttt cggatttgtc
      121 aacacactgg aagatgtatt gaaacgcgaa aaacccactc atatagccgt ggcttttgat
      181 cccaagggta acacttttcg gcacgaagca ttcaccgatt ataaagcaca acgcgaaaaa
      241 acgcctgaag atatccggtt atcggtgcca atcatcaaag aaatagtgaa agcatacaat
      301 gtaccggtgc tcgaagtgcc tggatttgaa gccgacgatg tgattggcac actggccaaa
      361 aaagccgaaa aagagggatt tgaagtgtat atgctcacac ccgacaagga ttacgggcag
      421 ctggtttcgg accacatttt catgtatcgc cccaaacata ccggaggcta cgaagtgatg
      481 ggacccgatg cggtgaaagc aaaatacgaa cttacaagtc acgaacaggt ggtggactta
      541 ttgggactga tgggcgatag ctcagacaat attcccggtt gtcccggagt aggtgaaaaa
      601 accgctgtaa aacttctcct ggaatttggc agcatcgata acctgctgaa taataccgac
      661 aaactgaaag gggcattaaa agtgaaaatt gaagaaaacc gggagcaaat ccttttttcc
      721 cgctatctgg ctaccattaa aacggatgta cccattgaac tggaggaaga aaaactgatc
      781 atggagccga tgaacgaatc ggctcttcgg gctttgttcg atgaacttga attcagaaca
      841 ctttctgcaa aaatttggaa tacgaccaaa aacaatgtcg ataaaaccgt atctccggca
      901 acaccagctc ccgaacacgg tcaaatgtcg ctcttcgata cgcccacttc cactgaaccc
      961 cgtcaggaag cgcaacgcga tcttttctcg cagtcgcccg atgttcaggt aattgccgga
     1021 atgcgttcca tcaatgaggt tccacataaa tacatactgg tagatgataa ggtaaaacga
     1081 gccgggctga tcaccaagct cttcagtcag aaatctgtct gcttcgatac cgaaactacc
     1141 ggaattgatg ttttcactgc cgacctggtg ggtttgtcgt tttgcttcac agccggtgaa
     1201 gcttattacg tgtcccttcc tgccggacga agcgaatgcc tggaggtaat gaaggagttt
     1261 aaggcatttt ttcagagcga tcagatccag aaaatcgggc aaaatataaa atttgacctg
     1321 cagatgctgg catcttacgg tatccagtta cgggggaata tgttcgatac aatgatcgct
     1381 cactatctgc tgcaacccga attacgccac ggaatggatt atctggccga agtttatctt
     1441 aagtaccgta ccatccatta cgaggatctg ttcgccggta aaggcaaaaa ccaacaggat
     1501 attcgcaggg tggatttgaa caggctgtgc gattatgcag ccgaagatgc cgatatcacc
     1561 tttcagctga agcaaatact ggaaactgaa ctgagccgta acgggttgga gaagcttttc
     1621 ttcgaaatgg aaatgccgct gttgcgggtg ctggctatca tggaacaaaa cggtgtaaga
     1681 attgatattg aagccctgcg tcaaagttcg gatatcttaa ctgccgatat gaaactattg
     1741 gagaaagaga tacatcaact ggtgggtaat gagttcaatg tgggttcgcc catgcaggtg
     1801 ggtgaagtgc ttttcggaca gctcaaactg gatgaaaagg caaaaaaaac caaaaccggg
     1861 caatattcca cctccgaaga tgtgctggaa aaacttcggt caaaacatcc cgtggtaagt
     1921 aaaattctgg attaccgggg gctaaaaaaa ttattgagta cctatattga cgccttgcca
     1981 ctgctggtca atgccaaaac gggtaaagtg cacactacct acaatcagac cgtggcggct
     2041 accgggcggc tgagttccac caaccccaac ctgcaaaaca ttcccgtgcg cgatgaactc
     2101 ggacgcgaaa ttcgcaaggc atttattccc aacgatggcg aattgttgct gagtgcagat
     2161 tattcgcaaa tcgaacttcg gataatggct cacctgagtg gcgacgccaa catgctcgaa
     2221 gctttcaaca gcggacagga tattcacgca gctactgcag ccaaaattta caaaattcca
     2281 ctggaggaag ttacatcgga tatgcgccgt aaagccaaaa cggctaattt cgggattatt
     2341 tacggcattt ctacctttgg tctgagtgag cgcctgggaa ttccacgagc agaagcaaaa
     2401 gagttaattg atggctattt tgagacctat ccggatatta aaagatacat ggaagaggtt
     2461 attcagcggg caaaggagca ggggtatgtg gagacaattt atggtcgtaa aagatatctc
     2521 cccgatatca attcacaaaa cagcgtggta cgcggatttg ctgaacgaaa tgccatcaat
     2581 gcaccgattc agggctcggc agcagatatt atcaaaatag cgatgattcg tatacaggaa
     2641 caactcgaaa agaaaaatct gaaagccact atgaccatgc aggtacatga tgaactaaat
     2701 ttcagtgtac caaaagacga actggaggta gtacgacaat tagttatcgg tgaaatgcag
     2761 caggctattc agctgaaagt gccgttgata gccgattgcg gagtcggtga caactggctc
     2821 gaagcacatt aggtggctgc ccggcaggga tatgacaaag aagccctttc tcaatcgaga
     2881 aagggcttct ttattatttt tcagaagcaa ttaagccagt ttgttaacgt agatggacag
     2941 cttggatttt aagttgcccg ctttgttttt gtggataacg ttgcgtttag ccagtttatc
     3001 aagcatagcg ctgatttttg gtaacatttc cacggctgca gctttttctg tagttgagcg
     3061 caacttgcga acagcattac gtgctgtttt tgcataatag cggttagcta atttccgttt
     3121 ttcagtctga cgaattcttt taagggatga tttatgattt gccatctcaa tctttttttt
     3181 agtctgttat ttttttttgt agcccatagg ggaatcgaac ccctctttcc agaatgaaaa
     3241 tctgacgtcc taaccgatag acgaatgggc cattgaaccg gaataatctc tatatccttg
     3301 ttttcaattg cgggtgcaaa tatacggcac ttttttgaat tgacaaagcc ttgtccgaaa
     3361 atattttgaa attttctgtc ataacttcag ataacgctga tattcaaccg cataaaaagt
     3421 gatggatttt tcctcattct aattattcct ttaactgatg gtcgaatttt gtggatttgc
     3481 attgtttagt tgctataaaa aaataaaaat gggaggtgtt gattacgtcc catttatatt
     3541 ttttggcggt atgagtgaga tttactgcat gaatcagagc agcgactcca cttttgcttt
     3601 cagggtcgct ttgggtgtag cacctatttg tttgtcggcc agcttgccat ccttgaagaa
     3661 aagaatggtg ggaatgttgc gaataccata ctcattggga gtgtcgatgt tctcatccac
     3721 gttcatttta ccgatttcca ctttgcctgc atattcttca gcgagttctt cgacaagcgg
     3781 gcccaccatg cgacagggac cgcaccattc tgcccagaaa tcgattacta cgggtttgcc
     3841 cgattcgata atttctttga tgttttcgtc tgtaaattct aaagtcataa ttttagtttt
     3901 ttagatagta atttttatta gtttaataac gctgttgact caaaaatgtt ttttggcata
     3961 aagcaaaatt ctttattttt tccggtaaat taagtcattt caattttata tcgcagtata
     4021 tcttcagctt cggcttgcct gaggtactga taaactgttg aatttatttt catccggtgt
     4081 ggccgggcaa agagtgaaat tttttcggag gttaaaaaat cctttatttc aatatataca
     4141 tttacatctc ccttgttttc ggttactatt ttggtgagtt ctacggccac atcgttggtc
     4201 agtttatcga gcgagagata aatccggatg cgggttatca gttttctaac ctctttgaag
     4261 ggcataatgt tcattatttt caattcaatt tcgggtttca catttggatc gggagtctgg
     4321 tttcggtaat cactcccttt tacctgtact actgcatgaa tatacaaaaa caggtctttg
     4381 atcatgtatt tggcgtaatc gtcaaaattt ttgccaaaaa gcggaaactc gtgcgagccg
     4441 ttgtaatctt cgagcgtgat catgccatag gggtttcctt ttttggtcat accgtcgcga
     4501 aaggcggtta ccattccccc tattttgagc cctgtgccca attgatagcg gctaaggtcc
     4561 ttgatatcct cggtggttgc attgcatata ttttttatct caaattcaaa ttcatccatg
     4621 ggatgagccg agaggtacat gccgatcatt tcgcgctcct tattcagttt caccagcggc
     4681 gaccactccg gagcatatgg cacttcgggt tttgtgattt gcaccgcatc ttcaagtgca
     4741 ccgaacagtg aattctgatt gaaagaaacg tcttgctgat agaggtttcc gtattttatg
     4801 aatgcatcca gtacgagttc ttcctttgaa ttcagtacaa aaaactgctc gcggcggatg
     4861 tcggtcagtg aatcgaaagc gcctgccagc accagacttt ctatattttt cttgttgcag
     4921 gcattgaggt taacccgttc aaaaaaatca aagttgtttt tgaatttgcc attcgtttca
     4981 cgttctttga taagtgcttc cacggctccc gctcctacat tttttattcc cgccagtccg
     5041 aaacggatat taccatcttt atttactgta aaagtaagat cactttcatt gatatccgga
     5101 ccgagtacat tcattttcaa tgatttgcat tcgtccatca gtttgccaac ttcggtgatg
     5161 tcgtccttgt ttctcgttaa gttggcggcc atgaattcgg cctcataatg agccttgagg
     5221 tatgccgtct gataagccac cagcgagtaa catgtggcgt gcgatttgtt gaaggcataa
     5281 gaagcgaatt tagcccagtc ggaccagatc tgttccagtt tttccacatc ggaatatccg
     5341 ttcttttgtg caccctgcag aaatttctca tgcagtgatt ccatcttgtc tttcagcttc
     5401 ttacccatcg ccttacgcaa ttcgtcggac tgaccacggg taaagcccgc caggtcgcgg
     5461 ctcagaagca tgacctgttc ctggtaaacg gtgatgccat aggtatcatt caaccttttt
     5521 tccatcaccg ggaaggcata ttctatcggt tcctggccat gtttgcggcg gataaactgc
     5581 ggtatataat ccattggtcc gggacgatag agcgcattca tggctatcag atcctcaaat
     5641 ttggtaggtt tcagttcctt cagatatttc tgcattccag ccgattcaaa ctggaacacg
     5701 cccaccgtat cgcctttgga gaatagttta taggtttttt tatcgtcgat gggaatgttg
     5761 tcaatatcaa tgtcaatccc tttggacttt ttgatgttga ccagggtgtc tttgatgatg
     5821 gacagcgttt tcagtccgag aaagtccatt ttcaccagcc cgacatcctc tatcactgtc
     5881 ccttcgaatt gggtgattac cttatcttct ttggtttcct tgtccaccgc ggtgctgaga
     5941 ggcaccaaat taatcaggtc gtcggctccg ataatcagtc cgcaggcgtg tacgccggtc
     6001 tgacgcaccg taccttcgag catttcagca tatttcagcg tgttggcaag ggttgggtcg
     6061 ttggagtcgc gtgcatctct cagttcactt acatatttga agcagtttgt cagattgact
     6121 ttaatttctt tgtcattttc atcgtttggg aatcttccgg gaatgagttt tgtaagtctg
     6181 tcggcttccg agagaggtaa tttctgaacg cgtgccacat cttttatggc tgatttagtt
     6241 cccatggtgc cgtacgtcac gatgttggcc acccgtttct ttccgtattt ttcggtcacc
     6301 cagcgcataa tctgcccccg tttatcatct tcaaaatcaa tatcgatatc gggcagtgaa
     6361 atacggtctg gattgaggaa acgctcaaac agcaaatcat acttcatcgg atctatattg
     6421 gtgattttca gacagtatgc caccaccgaa ccggctgcag agccacgtcc cgggcccacc
     6481 gagatattca tgtcgcgagc tgcctgtata aagtcctgca caataaggaa gtatccggga
     6541 aaacccattt ttttcatcac gtccagttca aaatcgatgc gttcccgtgt ctcatcattc
     6601 aaatttgctc cgtaacgcct ctctgcaccc tgataggtga gtttggtcag gtagtcggca
     6661 tccagtttta tacggatggc tttgtcgtaa ccgccgatgc ggtcaaaagc ttcgccgaac
     6721 tcttcgcgga gtgtcccttc atcgtatctc tgccggtaat cttcttcggt gccaaattca
     6781 gccggaattg ggaatacagg catcattgga gcagattcaa gtttgaagaa ttccacttta
     6841 ttggcaattt ccagtgtgtt ttcgagacat tctggaatat cggcaaaaat ttcagccatt
     6901 tgttccggtg ttttcagcca ctcctgtttg gtgtagcgca tgcgggtagg gtcatccagg
     6961 tccttgccgg tgctcagaca gatgaggtgt tcgtgggcat cggcatgatc ttcttccaca
     7021 aaatgtacat cgttcgaggc gatgagtttc gtattggtct tgcgggcaag agcaatcagc
     7081 tccttattct gaagttcctg tttgacatac gtttgtgtgt cggctcccgg tttgtctgtt
     7141 ttgtggcgtt gcagttcgag gtaataatcg tcgccaaaaa cttttttgta ccacagtatg
     7201 gctctttcgg cagcagccag gtcgccggct tcaatgtttt tgtgaatttc gccacccagg
     7261 caggccgatg tggctatcaa tccctcgctg tactttgcca gcaggtcatg gtcaatgcgc
     7321 gggcggtaat aaaatccctg aatccaaccg tttgaaacca gccggcaaag attgcggtat
     7381 ccggtttcat ttttagcaag cagtaccagg tgatagccgc ttcggtcacc tttatcttcc
     7441 ttgtcgtagc ggcttcgttg ggccacatac acttccgaac caaaaatggg ttttatcatt
     7501 tttagtttag taagttcaat ttccttacga atgtcctcct tctcctcttc gcccgtttcg
     7561 ggaactttca gtttccgttc cagtacggtg attttatctt ttaacgggcc atttttcttt
     7621 ttacaaacgt tgtaaaattc cttgatgccg aacatattcc cgtggtcggt cagtgccagc
     7681 gcgtgcatgc cgcttttcac agctttatcc actaaacctg atatgcttga catcccgtcg
     7741 agtacagaat agctggagtg aacgtgtaaa tgtacaaatg agcccatatt ttaatcccct
     7801 gataataagc gtaataaaaa attaattttt ggataaatcg cgaatgataa agatacaaga
     7861 atattctacc ggagtaaaat tttatccgaa aaagttattg acaatttgag caaaaacaaa
     7921 agaaatgatg agaatgaggg agatagcggg tattaaattt tcccaaataa ataccgttac
     7981 tgtgaaatcc tgttgcaaat atgaaatgtg tccaggggtg attcggtcta cattcaaatc
     8041 ctgtaaatgt ataatacaga tataaagcaa taaaattaca tatttaagta atatccccaa
     8101 accggatatg aatgaaatta gcttatagct ataaggaaga ccaagttctg tgaagttaac
     8161 agaactaatt atcgcctgct gactatcgat gcgatcatga gcctgcttta tttgtcaaag
     8221 taaaaaatca gaaaatgtca ggaagaaatg aacaaccgaa tgtccggtct ttttttatag
     8281 gataaaattt tacaatttct ttgctcatac tcctttataa agtggcaccg atgcctgatt
     8341 ttattacttt tttttgaggg taggcataaa gttgtgattt tacaatggtt aaactgccat
     8401 taatgagaac gttgaaatag ttttatttgc aagtgctggc gcttttcttt tagcttgaca
     8461 caggccggaa cattcggaag aagactacgc cgaaccggga cgaacttgtg tatatttttt
     8521 gatttaacgt tgtttaccgg attttatctg ttatttttgt atgttcaaaa aaagtaaaaa
     8581 tccaacatga tgacaaaacg aatgttgctc tatatactta cggcgactgc agcgttgttg
     8641 ttcgcagcgt gcaaccggca gcagaccgaa gccgactgga cgcatctcaa taagttggac
     8701 agcttgctcg cagtgcagcc cgaagctaca gccgacagtt tgaaacaaat ccaccccgcc
     8761 agactctcac atttcaaccg cggttattat caactcctga aggtgattgc cctggataaa
     8821 acctattaca attttacatc ggacagcctg atcgatgcaa cggtacacat cctgtcgcgt
     8881 cacaaacggg catacccgcg cacgtatgcc cgttccctgt tgtatcaggg actggtgcgt
     8941 taccgcatgg gtgtgaccga cagtacagct tacgaaccga taaaagaagc cgctgacata
     9001 ctggaagtaa agaaaattaa tgatccgctc ttattgtatt tttgctatca ttatttgggt
     9061 ttaatccaca acgaaaacaa taatccatca tacagtatcc tgtattatga gaaagccatt
     9121 aaaaagataa agcaatacgg agataaaaat tttctgttga tgacatattt tgaaattacc
     9181 tggacgtatc tgaagataaa caagattgac gcagccaaac agtatattga tactttaatt
     9241 aacgtcaaaa acataacgga tgcacaaaaa gcaagcataa atcaaattct ttcagtgtat
     9301 tatgacagca ccaatgaacc tttaaaggca ctggaaataa acaaacatct tgtaacgtca
     9361 gattacaatt acacggactt atccagcatg ttatttaaaa tttcaaaaaa ttataaggca
     9421 ttaaataaat tggacagcgc tttgcactat gccataaaag ccgaagaatg taaacccgat
     9481 tcggattatt atcttaataa tttgtattat aaaaatatcg gtgaaatatc cgaacagctt
     9541 aagttgtggc aaataagtgc aaatgcttat agaaaagcat atctgttgaa agaaaaagcg
     9601 acattaaaag ccctggataa agaaattatg tcgcttgaaa agaaatacga cttaaaggag
     9661 gcggaaaaca aagcccttca actgacgaac aggttgatat ttacaggctt ttttagttta
     9721 accctgctga ttttgcttgt cacttccttc gttatttttc gtcaacgcgg aaaacacgcc
     9781 caaataaaaa cgcagctgct ggcacaggaa aagataaatg ttgaacgaaa tttgattgaa
     9841 aaagagttta tgctacccat gtatcagcaa atatctcaac ggaatgccat cattaaagca
     9901 ttcttgtctg atttaatgac caatccgtat cttgctaaaa acacgcagat tttacaaaaa
     9961 atcagtgaaa tttatcagga ttctgtaaaa acatccaata taaatgaatt tcttacggat
    10021 gaaaaattca ccgaatttac cgggattgaa tgtgaaagtt gccagttgct gaatgaaaac
    10081 gagaagatgc tgcttgtttt tgccgcaatg aaacttgaca accggcagat tgccgttctc
    10141 tttaatacaa cagaatcgag cgttcgcgga cgaaaagcga agcttagagt gaagattgaa
    10201 aacaaaaata ttgacataaa aggcattatc atctgatttt tacccctcag aacgaccgtt
    10261 actttcattt tcttaattat tgctatacag ccatttacgc ttattttatg cgttactttc
    10321 ctttcacacc cgcaaaactc ttgcgggtat tttatattta agatacctta gcagacacaa
    10381 actcaaaaaa aattacaata taaactttta aaaaaattac aacatgaaga atctcatttt
    10441 tcgctttatc ctgatggtat ttgtttttgg aatatcttcc gtttcgctcc atgccggaga
    10501 tgtaaactat gaacaagaaa ttgaatttag aaaacagaat cctgcaggtc agccccgtgc
    10561 tccatcccgg taccctgtaa gcttcaccgg cacttatttt atggacgaat taattgttgg
    10621 ttctaaaaat tatactggtg aaattaccgt acaaattaca ggcataagtg gaagtctaat
    10681 tcaagctgtt cagatttcgg catccgaata tttcaccatt gatcttagct ctctgccaga
    10741 aggagtgtac gatattgcta ttattaccga aggtaaaggt acatttaacg gagtatttga
    10801 gaagtaatat catcagagcc ttttcaaggc tttctatctt attgtctcat ttattttcgc
    10861 aatctgtaag ttaaaaaaat cacaaaagtt cgacgtagct taagctgcgt cgaacataac
    10921 ttccggattt tgtccggaca cgaacttaaa atcccgggat aacgccgaat cctgagatgt
    10981 gtggaaggac tattctgaaa gtccgaataa aaaaaaagac tttccggttt gttaattatg
    11041 aaatctaatt tagttaaaca taacaattaa tactatctat catgaaaaaa ttcattttta
    11101 ttttatcgtt aacgacttta ttaataagtt gtaatttgga tactgcgcct ggtttattga
    11161 tggaaaccga gtcaatattc ctctttcatg gagaaacagc tgtattagga gcttcctctg
    11221 aaaacggatt ggtttactat aaaagttcgg atagtttagt agcagaagta gacgaaaaag
    11281 gggttattac tgccaatgtt gtaggaacta cctatattac agttttggat ggcaaggaag
    11341 ctaaagcttg taaggtcact gttacaccac gatttcttgt acccattaat ccatatgtta
    11401 attttggagc ctcggtaagt gaagtaaaaa aagcagtaaa agacgttttt atcgaagaac
    11461 tttcaaagat taataatagt tccgaaaaaa ccttggttta ctacaatcag aaagaaaatg
    11521 aaaaatcccg cattgcctat gaatatcgct ttttgaatga caaattaatt tctacaggtg
    11581 tttatgtaaa caaggatttt gaatatttcg ataatatgat ggattatctg gatgaaacat
    11641 attattacaa agatgaattt aaaaccacag ccgccagctc agcgctttcc tgtcaaagaa
    11701 tatatcaatg cgtcgaacaa aaagttgatc tgaaagtata ttataaaatt ccggtaacag
    11761 aatattacct tcttttttca gaaaaaaatt agatgcacta ccactgtaaa gtgtggtgtg
    11821 ttatttaaca gaaagaatgt tcggcgtagc ttatagggtt catgggagtt gaattcggca
    11881 aactcagccc cgacaaaata taggtaccag caaccagtgc aaaactattt acaataaaaa
    11941 aacatatccc gagagacgga gcgagccgat ttattaaaga gtttaaaata aaaatgcctt
    12001 cccttgccag gaaagcgaaa caacataaat tgagaccaat caacccgatg taccactaac
    12061 taacaagaaa aggcaacaca aaaataaata aaaacacggt aatatgcaaa ataataccgg
    12121 acgccttctc aaaaacgaag aatagtagga agtagagtgg cgcagctgtt cgggctgtgt
    12181 aaaaccaaac ggcgagtaac ccgaaaagcg aagtgagtag agtcgttttt aattaagat
//
LOCUS       DAOPZK010000058        11972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_6009_length_11972_cov_23.736427, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000058 DAOPZK010000000
VERSION     DAOPZK010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6009_length_11972_cov_23.736427"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKH68_05060"
     CDS             <1..350
                     /locus_tag="PKH68_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNX12339.1"
                     /translation="SGLSFYIWSFALIVVTARTVQFIMNQNSVNYTTEILVAAGALVA
                     CIGQFLLGKKIGKKYNDTIAGGQSLGQKNTILAIWMAQTFLNPISSIGPGSYVLWQNI
                     FNSYQVWRKRKSL"
     gene            380..961
                     /locus_tag="PKH68_05065"
     CDS             380..961
                     /locus_tag="PKH68_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNX12340.1"
                     /translation="MENGFFENRVIEKGDFSNIPLKKGEYENCSFVGCNFNNADLAEI
                     IFTDCLFSGCNLSLANLTKTAIRNTKFDGCKMLGLNFENCNKFGLSLRFENCQLNHAS
                     FYQIQLKNTVFRSCQLQETDFTESNLTDSLFDECDLYRAVFNQSNLEKVDFRTSKNYA
                     IDPENNRIKRAKFSYPDVLGLLDKYDIETDRLE"
     gene            1035..2351
                     /locus_tag="PKH68_05070"
     CDS             1035..2351
                     /locus_tag="PKH68_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNX12341.1"
                     /translation="MNWNQLLSPVRLGMENYHDRNRDNRNEFQRDYDRLIFSAPFRRL
                     QNKTQVFPLPGSVFVHNRLTHSLEVASVGRSLGMNVSQSLAATEPGYVDQIGFIVSAA
                     CLAHDLGNPPFGHSGEEAIATFFSEGKGQFLKNEMSEVEWNDCIHFEGNANAFRLLAH
                     QFNGRRPGGFVLTYSTLASIVKYPYSSVYADQKKQKFGFFEAERADFEKIAQELGIVR
                     QPDNPGRYARYPLVYLVEAADDICYQIMDIEDAHKLKILTTDETKDLFFSFFDDAKRK
                     KREETLNMVSDVNEQIAYLRSSVIGQLIDECSRTFIENENAILSGTFNGSLIKHLTGN
                     SATAYNRCSEIACKKIYRSQEVLDIELGGYKIILTLMDLFVNAVLQPEKAYSRQLINR
                     IPQQYDIHNPSCYGKVLAVLDYISGMTDVYALDLYRKITGINIVSM"
     gene            complement(2361..3812)
                     /gene="rodA"
                     /locus_tag="PKH68_05075"
     CDS             complement(2361..3812)
                     /gene="rodA"
                     /locus_tag="PKH68_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNX12342.1"
                     /translation="MSRTISMKYALDWTTVGIYLFLVFFGWLNIYGASYDFDQSSIFD
                     FSQRAGKQFIWILTGFGLAGAILLIDSRLYSFFSYLFYVAILLLLIVTIFVAADVKGS
                     RSWLEIGPVSFQPAELAKTATALALAKFMSSYNYKLKTWKDLIPLGVIVFIPFALIIL
                     QQETGSALVFLAFMLMFYREGMKGLVLLLVVLAVVLFVVAIGFSQTFIQVDRGTVGMV
                     MAMFIILLIQFCYAFFFNRHRKEALNLALGAGFIAVASYIVNIWVKVNYDYIAMGTVI
                     LSTVYWIIIEMFNRYKKYFLLAVISFGSLLFCFSADYIFNEVLEPHQQVRIKVLLNMD
                     EDLAGAGYNVNQSKIAIGSGGFLGKGFLNGTQTKLKYVPEQDTDFIFCTVGEEWGFVG
                     SSAVLLLYLWLLFRIVQIAERQRDAFNRIYGYCVASIFFFHLAINVGMVLGIMPVIGI
                     PLPFLSYGGSSLWSFTILLFILLRLDAARRTGS"
     gene            complement(3796..5631)
                     /gene="mrdA"
                     /locus_tag="PKH68_05080"
     CDS             complement(3796..5631)
                     /gene="mrdA"
                     /locus_tag="PKH68_05080"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNX12343.1"
                     /translation="MINDKLRNRGTVITIIMAVVVVIYILRLFSLQIVETKYKRFADS
                     NAFLNKTIYPPRGLIYDRNDSLLVYNKPAYDITFINREIHGLDTATFCSDLNITLDAF
                     KEKMKEVKDRDKNPGYSSFTPQVFMTQLDIRDIANIQQSMYKYPGFYIQTRTLREYRY
                     NAGAHVLGSIGEVSPKMIEKDDYYKRGDFAGRDGIEYTYEKELRGEKGVEIILRDARG
                     RLKGRYENGDKDIAPKAGKNLKLTLDIRIQLLAEQLLAGKVGSVVAIEPKTGEILALV
                     SNPTFNPSLLVGRERSKNYNLLVNDPTKPLMNRATQAQYSPGSTFKPFQALFGLQLGG
                     ITEGTRFACNGPSSWPIKCTHHHGSPVSLLNAIEQSCNPYFWNTFRSILEKDGYGDKN
                     VNFKNNYDKWREAVMAFGFGQKFEDTDIYQQSRGNIPSEKFYDKYFGKTGWRANTIRS
                     LSIGQGEVLVTPLQLANGLAAIANRGYYITPHLNKSDSMLTHKHKTSIDAKYFAIVDE
                     GMWRVNEFGTGRAYKIPGVSSCGKTGTVQNNRGKDHALYVGYAPRENPKIAIAVVVEN
                     SGFGSTWAAPIASLLMEQYILHKIERIELLNRISTSRTNANVTDY"
     gene            complement(5634..6131)
                     /gene="mreD"
                     /locus_tag="PKH68_05085"
     CDS             complement(5634..6131)
                     /gene="mreD"
                     /locus_tag="PKH68_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNX12344.1"
                     /translation="MAVALQNILRFIFLILLQVFILDNIQFMGYINPMIYVLFILSLP
                     VRFPKWALLILAFIMGLIIDVFSNTAGMHSFALVFAAFLRTPVINIFTSIDEGSNPTP
                     SFHTFGVSAYVKYVVILVLIHHFILFFIESFSFLHLTLLIPKIIISSTVTILLILGLQ
                     SFKSK"
     gene            complement(6135..6998)
                     /gene="mreC"
                     /locus_tag="PKH68_05090"
     CDS             complement(6135..6998)
                     /gene="mreC"
                     /locus_tag="PKH68_05090"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNX12345.1"
                     /translation="MKNLINFLIRYSVVFLFVALEILSLVLLSNNKGYQRSIMLSSGN
                     TLVGRMYEATNAFVEFFKLSEANDNLAIENTQLKNRVVELENKLNSLTDTLNNVSWKN
                     IRISPENEYAYITAKVIRNTTHLTQNYITLNKGSLEGIAPDMGVIGDDGVVGIVKSVS
                     PKFSVVIPILNPKIQISSKFKKNNYSGPLVWNGKDSRFASLNDIARHVRFSLGDTIVT
                     SGLTKNFPGGILVGTINDYNIKESDAYYNIQVKLAVNYRTLTHVKVINYKNYSEQSAL
                     EKQVTDEDKKK"
     gene            complement(7103..8122)
                     /locus_tag="PKH68_05095"
     CDS             complement(7103..8122)
                     /locus_tag="PKH68_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNX12346.1"
                     /translation="MGLFSFTQEIAIDLGTANTIIIHDDKIVVDEPSVVALDRRTDKL
                     IAIGEKARQMQGKENEGIRTVRPLRDGVIADFFAAELMIRGMIKMIPSKNHLFSPTLR
                     MVVCIPSGSTEVEIRAVRDSSEHAGGRDVYMIYEPMAAAIGIGIDVEAPTGCMIVDIG
                     GGTTEIAVISLGGIVSNKSIRIAGDDLTADIMEYMGRMHNIKVGERTAENIKINVGAA
                     LTDLEDAPEDYIVRGPNRMTALPMEVPVSYQEIAHCLEKSISKIEAAVLSALEQTPPE
                     LYADIVEKGIYLAGGGALLRGLDKRLTDKLNIQFHIADDPLHAVARGTGIALKNVDKF
                     GFLIR"
     gene            complement(8141..9661)
                     /gene="purH"
                     /locus_tag="PKH68_05100"
     CDS             complement(8141..9661)
                     /gene="purH"
                     /locus_tag="PKH68_05100"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNX12347.1"
                     /translation="MEIKQIKRALISVYHKDKLDEIIKKLHSEGVTFLSTGGTKSFIE
                     SLGIPCDAVEDLTSYPSILGGRVKTLHPKVFGGILARRDLEQDLVQLEQYEIPQIDLV
                     IVDLYPFEATIAAGADEATSIEKIDIGGISLIRAAAKNFRDVVILASEAQYNPFLELI
                     NQKGANTTLEERRWFAKEAFSVSSHYDTAIFNYFDGDNASAFRYSADGAKALRYGENP
                     HQKGYFFGKFDEMFEQLHGKEISYNNLLDIDAAVSLINDFEDITFAILKHNNACGLAS
                     RPTLKDAWEAALAGDPVSAFGGVLITNAIVDKETAEEINKLFLEVIIAPDYDLDALEV
                     LTQKKNRIILVLEGNKVPGKQFRSVLNGVLVQDKDLHTESAEELKVVTEKEPTGDEIE
                     DMLFANKIVKHSKSNAIVLARGKQLIASGVGQTSRVDALKQAIEKAKSFEFELEGAVM
                     ASDAFFPFSDCVEIAAGAGITAVIQPGGSVRDDESIEMCNKKGISMVTTGIRHFKH"
     gene            complement(9935..10930)
                     /gene="trpS"
                     /locus_tag="PKH68_05105"
     CDS             complement(9935..10930)
                     /gene="trpS"
                     /locus_tag="PKH68_05105"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNX12348.1"
                     /translation="MKTVLSGIRPTGNLHLGNYFGALSNFVSMQYEYNCFFFIADYHS
                     LTTHPTPQNIQGNVKSVLAQYLAAGLDPEKATLYIQSDVPEVIELYLYLNMNAYVGEL
                     ERCTSFKDKIRQQPQNVNAGLLTYPTLMAADILIHKADFVPVGKDQEQHLEMARTFGN
                     RFNRMYNHEYFPEPQAFNFGKELVKIPGLNGAGKMGKSEGEDSTIFLTDTPEVILKKV
                     KRAVTDAGPTEPDQPMTEPIANLFQLMNVVSKPETIQFFREKYNDCSIRYGDLKKQLA
                     EDMITFVNPIRERIQEIASDEVYLNKVALLGKEKAQESARKTIREVREIIGFKPF"
     gene            complement(11097..11552)
                     /locus_tag="PKH68_05110"
     CDS             complement(11097..11552)
                     /locus_tag="PKH68_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNX12349.1"
                     /translation="MEKIDKLDRKILRIISQNARKPFKDVAEECEVSRAAIHQRVQRL
                     IDMGVITGSGYSVNPKMLGFQICVYIGITLERASMYKDVVAELEKIPEVVESQYTLGA
                     YTILIKMYAKNDEHLMELLNGKLQEIPGVATTETLTSLDQRIKRTIPIE"
     gene            complement(11743..>11972)
                     /locus_tag="PKH68_05115"
     CDS             complement(11743..>11972)
                     /locus_tag="PKH68_05115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX12350.1"
                     /translation="LDGTEFDSSIKRNEPAMFPVNQVIPGWTEGLQLMPVGSKYKFYI
                     PQNLAYGSKGARGIEPLSTLVFEVELLSIEK"
BASE COUNT         3392 a         2574 c         2427 g         3579 t
ORIGIN      
        1 tctcgggact ttcattttat atctggagtt ttgcacttat agtggttaca gcccgaacgg
       61 ttcaatttat catgaaccaa aacagtgtaa attataccac cgaaatcctg gtagcagcag
      121 gagcactggt ggcttgtatc ggtcaatttt tactcgggaa aaaaatcggg aaaaaataca
      181 acgacaccat agccggtgga caaagtctgg gacagaaaaa caccattttg gctatctgga
      241 tggcacaaac gtttctcaat cccatatctt ccatcggacc gggctcgtat gtgctctggc
      301 agaatatatt taattcgtat caggtctggc gaaaaagaaa gtcgctatga cggtttatca
      361 aatcaaataa acagctggaa tggaaaatgg attttttgaa aatagggtaa ttgaaaaagg
      421 tgatttcagt aatattccgc tgaaaaaagg ggagtatgaa aactgcagtt ttgttggctg
      481 taacttcaat aacgccgacc ttgcggaaat tatttttacc gactgccttt tttcgggatg
      541 taatttaagc ctggccaatc tgacaaaaac ggccatccga aacactaaat ttgatggttg
      601 caaaatgttg ggactgaatt ttgaaaactg caataaattc ggattatctc tgcgttttga
      661 aaattgccag ctcaatcatg cttcattcta ccaaatccaa ctcaaaaata ccgttttcag
      721 gagctgccaa ctgcaggaaa ctgattttac cgaatcgaat ttgaccgact cattatttga
      781 cgaatgcgat ttgtaccgtg ctgtgtttaa tcagtctaat cttgaaaagg tagatttcag
      841 gacttccaaa aattacgcta tcgatcctga aaataaccgg attaaaagag caaaattctc
      901 ttatcctgat gttttgggac ttttggataa atatgacatt gaaactgaca ggttggaata
      961 ataatgatat cccacctgat gcatctttcc ccgaaatttt gtaattttac atttaatcaa
     1021 caagcatcat ggccatgaac tggaatcaac tcttatcacc cgtacgtctg ggtatggaaa
     1081 actatcacga ccgcaaccgc gataaccgga atgaatttca gcgggattat gatcggttga
     1141 ttttctcggc tccttttcgc cggttgcaaa acaaaacgca ggtttttccg cttcccggaa
     1201 gcgtatttgt acacaaccgg ctcacacaca gccttgaagt ggccagcgta ggccgttcgc
     1261 tggggatgaa tgtatcgcag tcgctggctg caaccgaacc gggatacgtg gaccagattg
     1321 gtttcattgt atcggctgcc tgtctggctc atgatttggg caatccacca ttcggacatt
     1381 cgggcgaaga agccattgcc actttttttt cggaaggaaa agggcaattt ctgaaaaatg
     1441 aaatgtcgga agtggaatgg aatgactgca tccatttcga gggaaatgcc aatgctttcc
     1501 gcttactggc tcatcagttc aacggacgcc gtccgggtgg ttttgtgctc acttacagca
     1561 cactggcttc tattgtcaaa tatccgtatt cttcggttta tgccgatcaa aaaaaacaaa
     1621 aatttggttt ttttgaggcc gaacgagctg attttgaaaa aattgcacaa gaacttggta
     1681 ttgtcagaca acccgacaac cccggccgat acgcccgcta cccgctggta tatctggtgg
     1741 aagcagccga cgatatctgc tatcagataa tggatattga agatgcacac aaactgaaaa
     1801 tactgaccac cgacgaaacg aaggatttgt ttttcagctt ttttgacgat gctaaacgaa
     1861 aaaaacggga agaaacactc aacatggtga gtgatgtcaa cgaacaaatt gcttatctgc
     1921 gctcatcggt tatcggacaa ttgattgatg aatgctcccg tacgtttatt gaaaatgaaa
     1981 acgctatact cagcgggact tttaatggtt cattgataaa acacctgaca ggaaattctg
     2041 caactgcgta taaccgatgt tcggagattg catgcaaaaa aatttaccgc tcacaggaag
     2101 tgctggatat tgaattagga gggtataaga ttattctcac gttgatggat ctttttgtaa
     2161 atgccgtact acaacccgaa aaagcgtatt ccagacagct tattaaccgc attccgcaac
     2221 aatacgacat ccacaatcca tcctgttatg gcaaagtatt agccgtgctc gattatattt
     2281 ccggcatgac cgacgtatat gcgctcgatt tataccgcaa aattacgggg ataaatattg
     2341 tcagtatgta gtgtggttta ttaactgcct gttcgccggg cagcatccag ccggaggagt
     2401 ataaacaaca gaatggtaaa gctccagagt gacgagccgc cataacttag aaatggcaaa
     2461 ggaattccga ttacaggcat aatcccaagc accataccca cattgatagc caggtggaag
     2521 aaaaatatac tggctacgca ataaccgtag atgcggttaa aagcatcccg ctgtcgttcg
     2581 gcaatctgaa caatgcgaaa aagtaaccag agataaagca gcaacactgc cgacgaccct
     2641 acaaaacccc actcctcacc taccgtacag aaaataaagt cggtatcctg ctccggaaca
     2701 tatttcaact tagtctgagt tccattcaga aaaccctttc ctaaaaatcc accggaacct
     2761 atggcaattt tcgactggtt tacattgtaa ccggcacctg ccagatcctc atccatattg
     2821 agcaaaacct taatacggac ctgttggtgt ggctccagca cttcattgaa aatataatcg
     2881 gcggaaaaac aaaataataa ggagccgaaa gaaatcactg ccagcaaaaa gtatttttta
     2941 tagcggttaa acatctcaat aataatccaa tacacagtgg ataggattac ggtgcccatg
     3001 gctatgtagt cataattgac tttaacccaa atattgacga tataagaagc gacggctatg
     3061 aaccctgctc ccaaagcaag attcaacgcc tcttttcgat gacggttgaa gaaaaatgcg
     3121 taacaaaact gtattaataa aataataaac atggccatga ccatacccac agtgccccgg
     3181 tcaacttgaa taaatgtctg gctgaagcca atggcaacca caaataaaac cactgccaaa
     3241 acgaccagca ggagaacaag acccttcatt ccttcgcggt aaaacatgag cataaaagcc
     3301 aaaaacacca gcgcacttcc ggtttcttgc tgaagaataa taagtgcaaa aggtatgaaa
     3361 actataacgc cgagaggaat tagatctttc caggttttca gcttataatt ataggagctc
     3421 ataaatttag ccaaagcaag cgctgtagct gttttggcca gttcggcggg ctgaaagctt
     3481 acaggaccta tttcaagcca tgatcgggag ccttttacat cggcagcaac aaagatggta
     3541 acaatcaaca gcagaagtat tgccacgtaa aataaatatg aaaaaaaact gtaaagtcgc
     3601 gaatcaataa gcagaatagc tcctgccaga ccgaaacccg ttagtatcca gataaactgt
     3661 ttgccggcac gctgactgaa atcgaatata ctcgactgat cgaagtcata acttgccccg
     3721 taaatattaa gccagccaaa aaagaccaga aacaggtaga tacccaccgt agtccagtcc
     3781 aacgcgtatt tcatgctaat agtccgtgac attggcgttt gttcttgagg ttgaaattct
     3841 gtttaataac tcgattcgtt caatcttgtg taaaatgtat tgctccatca ataaactggc
     3901 aataggagca gcccaggtgg aaccaaaacc ggagttttcg actaccactg caatggcaat
     3961 tttaggattt tcgcgtggtg cataccccac atataatgca tggtcttttc ctctgttatt
     4021 ctgaacggta cccgttttac cacaactgct tactccgggt attttgtacg ctctccccgt
     4081 accaaactca tttacccgcc acattccttc atctacaatg gcaaaatatt tagcatcaat
     4141 tgatgttttg tgtttgtgcg tcaacatcga gtccgattta ttgagatgag gggtaatata
     4201 atatcctcta ttagctatgg cagccagtcc gttggccaac tgcaggggtg taaccaacac
     4261 ttcgccctgt ccgatcgata gtgaacggat ggtattggcc cgccaaccgg ttttaccaaa
     4321 atatttatca taaaatttct ctgatggaat atttcccctg ctctgctgat agatatccgt
     4381 atcttcaaat ttctgcccga aaccgaatgc catcactgcc tcacgccatt tgtcgtagtt
     4441 atttttgaaa tttacatttt tatcaccgta gccatccttt tcgagaatgg atcgaaatgt
     4501 attccagaaa tagggattac aactttgttc gatggcattg agcaacgata ccggcgatcc
     4561 atggtggtgg gtacatttta taggccatga agatggtccg ttgcacgcaa aacgtgtacc
     4621 ttcggtaata ccacccaact gaagtccaaa tagcgcctga aagggcttaa aagtagaacc
     4681 gggtgaatac tgagcctgtg tagccctgtt catgagtggt ttggtggggt cgttgacaag
     4741 cagattatag tttttggatc tctcccgtcc taccagtaac gagggattaa acgttggatt
     4801 ggaaaccaat gcaagaattt ctccggtttt gggttctata gctacgacac ttcccacttt
     4861 accggcaagc aattgctcgg caagtaattg tatcctgata tccagtgtga gtttcagatt
     4921 tttgccggct ttgggagcta tatccttgtc tccgttttcg tatcttccct tcaatcgtcc
     4981 acgtgcatca cgcagaatta tttcaacccc tttttcacca cgcagttctt tttcgtaggt
     5041 gtattctatt ccgtctcttc cggcaaaatc accgcgtttg taataatcat ctttctcaat
     5101 catttttggc gaaacttcac cgatgctgcc cagtacatga gcacctgcgt tgtaacggta
     5161 ttcccgcaac gttctggttt ggatataaaa accgggatat ttatacatcg attgctgtat
     5221 attggctata tcgcggatgt cgagctgagt catgaaaacc tgaggtgtaa acgatgagta
     5281 acccggattt ttatcccgat ccttcacttc tttcattttc tccttgaagg catccagggt
     5341 gatattcaga tcactgcaaa aagtggccgt atcgagtccg tgaatttcgc gattgataaa
     5401 agtgatgtcg taagccggtt tgttatatac gagcaaagaa tcattccggt cgtaaatcaa
     5461 cccccgggga ggataaatgg ttttattcag aaaggcatta ctatcggcaa accgtttata
     5521 cttggtttcg acaatctgca atgaaaaaag ccgtaaaata taaataacca ctaccacagc
     5581 cataatgata gtaattacgg tccctctgtt tcgaagctta tcgtttatca tgcttatttc
     5641 gacttaaagg attgcaatcc gaggatcaac aggatagtta ctgtcgagct gataattatt
     5701 ttcgggatca gtaaagtgag gtgcaggaag gagaatgatt cgatgaaaaa caaaataaaa
     5761 tgatgaatca acacaaggat aaccacatat ttaacataag ccgatacccc gaaagtatga
     5821 aatgaaggag taggatttga accttcatcg attgatgtga aaatattgat aaccggtgta
     5881 cgtagaaaag ctgcaaaaac aagtgcaaag gaatgcattc cggctgtatt ggaaaacaca
     5941 tcgataatca atcccatgat aaaggcaaga ataagcaatg cccatttggg gaaacgaacc
     6001 ggaagtgaaa gaataaacaa aacgtatatc atcggattga tatatcccat gaattgaata
     6061 ttgtccagta taaacacctg aagcaaaatc agaaatataa accttaatat gttttgtaat
     6121 gctaccgcca tgggttattt ctttttatct tcatctgtaa cctgtttttc aagcgcactc
     6181 tgttcgctgt aatttttgta gttgatcacc ttcacgtgtg tcaatgtccg gtaattgacc
     6241 gccagtttta cctgaatatt gtagtaggca tcactttcct tgatgttgta gtcgtttatg
     6301 gtccctacca gaataccgcc ggggaaattt tttgtcaatc cgctggttac aatcgtatca
     6361 ccgagactga accgaacatg ccgggcaatg tcgttcagac tggcaaaccg tgagtctttt
     6421 ccattccaca ccaatggacc ggaataatta ttctttttaa acttactgct gatttgaatt
     6481 tttgggttca ggattggaat aacgaccgaa aatttgggtg aaacactttt tacaatacct
     6541 acaaccccat catcgccaat aactcccata tcgggtgcta ttccttccag tgagccctta
     6601 ttgagcgtta tatagttttg ggtgagatgt gtggtgtttc ttataacctt tgcagtgatg
     6661 taggcatatt cgttttcagg tgaaatgcgg atatttttcc aacttacatt gttgagcgta
     6721 tcggtgagtg aattaagttt attttccagt tccactaccc ggtttttgag ctgggtgttt
     6781 tcaatagcta aattatcatt ggcttcactc agtttgaaaa attccacaaa tgcattcgta
     6841 gcttcataca ttcgtcccac caatgtattt cccgatgata gcatgatgct ccgctgatac
     6901 cctttattat tggaaagcag aacaagtgag aggatttcta atgcaacaaa aagaaatacc
     6961 acactgtatc ttatcagaaa attgattaga tttttcaaaa gaaaatataa ctatcgatta
     7021 ttttaaaaaa cacacgacaa acagcttgtt tctcgtgtgt ttatttattt acctgaatat
     7081 ttttttgctg aattacaaac gattatctga tcaggaatcc gaatttatcc acatttttga
     7141 gcgctattcc ggtaccgcgg gccactgcat gcagcggatc gtcggctata tgaaactgga
     7201 tgttcagttt atcggtcagt cgcttgtcaa gtccgcgcag caaagcacca ccaccggcca
     7261 gatatatacc tttttccacg atatcggcat acagctcggg aggagtttgt tccagcgcac
     7321 taagcacagc tgcttcaatt tttgaaattg atttttcgag acaatgggca atttcctgat
     7381 aagataccgg aacttccatg ggcaaagccg tcatacggtt tggtccgcga acgatgtagt
     7441 cctcaggagc atcttcgagg tctgtaaggg cagcacctac gtttattttg atattttcag
     7501 cggtgcgttc acctactttg atgttgtgca tacgccccat atattccatt atatcggctg
     7561 tgagatcatc tccggctata cgtatggatt tgttcgaaac gatgccaccg agtgaaatta
     7621 cagcaatttc ggtagttcca ccgcctatat ccacaatcat acatcctgta ggtgcttcca
     7681 cgtcgattcc gataccgata gcagcagcca ttggttcata aatcatatac acatcgcgtc
     7741 caccggcatg ttcagaagag tcacgtacgg cgcggatttc cacttcagta cttcccgaag
     7801 gaatacaaac caccattcgg agcgttggtg agaataaatg attcttggaa ggtatcattt
     7861 tgatcattcc ccttatcatc agttcggcag cgaaaaagtc agcaattacg ccgtcacgaa
     7921 gtgggcgaac cgtgcgaatt ccttcgtttt ccttaccctg catctggcgt gccttttcgc
     7981 cgatagctat cagtttatcg gttcggcggt caagtgccac aaccgatggt tcgtccacaa
     8041 ctattttatc atcatgaatt ataatcgtat tagctgttcc taagtctata gctatttcct
     8101 gcgtaaatga aaaaagtccc atatatataa atttattttt ttaatgttta aaatgcctta
     8161 tcccggtagt aaccattgat atgccttttt tattgcacat ttctatgctc tcatcatcgc
     8221 gaaccgaacc tccgggctga atcacggcag taattcccgc cccggcagct atctccacac
     8281 aatcggagaa agggaaaaag gcatcggagg ccatcactgc gccttcaagc tcaaattcaa
     8341 atgattttgc tttttcgata gcctgtttaa gagcgtcaac acgtgatgtt tgtcctactc
     8401 cgctggctat cagttgtttt cctctggcca aaacgattgc atttgatttg gaatgtttta
     8461 caattttatt tgcaaaaagc atgtcctcaa tttcatcacc tgtgggttct ttttcggtta
     8521 caacttttag ttcttcagcc gattcggtat gtaaatcctt gtcctgcacc aatactccat
     8581 tcagcaccga acggaattgt tttccgggaa ctttgtttcc ttccaaaacc agtataatcc
     8641 ggtttttctt ttgtgtcaat acttccagtg catccaggtc gtagtcggga gcaatgatta
     8701 cctccagaaa taatttatta atttcttccg ccgtttcctt gtctacaatg gcattggtaa
     8761 tcagtacgcc accgaaagcc gataccggat cgccagccag tgcagcttcc caggcatctt
     8821 tcagcgtggg ccgcgatgcc agaccacagg cattattatg tttcagaata gcaaaggtaa
     8881 tatcttcaaa atcatttatc aaacttacgg cagcgtcaat atccagaaga ttgttgtacg
     8941 aaatttcctt gccgtgcagc tgctcaaaca tttcatcaaa tttgccgaaa aagtaaccct
     9001 tctgatgcgg attttctccg taacgaagtg ctttggctcc atcggcacta taccggaaag
     9061 cgcttgcatt atcgccgtcg aaataattga aaatagccgt atcgtagtgt gaagaaacgc
     9121 tgaaagcttc cttggcaaac caccggcgtt cttccagagt agtgttggcg cctttctggt
     9181 tgattaattc cagaaatgga ttatattgtg cttcggaggc aagaattaca acatctctga
     9241 agtttttggc ggcagcccgg ataagtgata ttccacctat gtcgattttt tcgatggaag
     9301 ttgcttcatc ggctccggca gcgatagttg cttcaaaagg gtataaatct acaatcacca
     9361 gatcaatctg aggaatttcg tattgttcca gttgaaccaa atcctgctcc aaatcgcggc
     9421 gggcaagtat tccgccaaaa accttggggt gtagggtttt tactctacct cccagtatcg
     9481 atggatagga agtcagatcc tccactgcat cgcagggtat tcccaacgat tcgataaatg
     9541 attttgttcc accggtagat aaaaaggtga caccttctga gtgaagtttt ttgataattt
     9601 cgtctaattt atccttatga taaactgata tcagcgcccg tttgatttgt tttatttcca
     9661 tacggttttt tagtgaggat tttggagtgc aaatctacga aaaatcattg agttagcaat
     9721 tctttttgcg tggttttaat ctatttttag agtgacatca gttactcgct cttttaatca
     9781 tggaattaat ctgtttctga atttattact gttttcaaat agaaaaatcc ccggaaattg
     9841 aattattttt ttccatttga atgtacgtat tttcaacttt ctgagaaatc catttatcta
     9901 aaaatagcca acgttttaga cattggctac cttatcagaa gggtttaaac ccgatgattt
     9961 cacgcacttc gcgaatggtt tttcgggcac tttcctgcgc tttttcttta cccagcagcg
    10021 ctaccttatt gaggtaaact tcgtcacttg ctatttcctg tattcgttca cgaatcgggt
    10081 ttacaaatgt gatcatgtct tctgccagct gtttttttaa atcaccgtaa cggatactgc
    10141 agtcgttgta tttttcccgg aaaaactgaa tggtttcggg ttttgaaacc acattcatca
    10201 actgaaataa gttggcaatg ggctctgtca tcggttgatc gggttcagtc ggcccggcgt
    10261 cggttacagc tcgttttact tttttgagga tcacttcggg ggtgtctgtc agaaaaatgg
    10321 tgctgtcctc cccttccgat tttcccattt ttccggctcc gttgagtccg ggtattttca
    10381 ccagctcttt accaaagttg aacgcctgag gttccggaaa atattcatgg ttgtacattc
    10441 ggttaaatcg gtttccaaac gtgcgtgcca tttccaggtg ttgctcctga tcttttccca
    10501 cgggaacaaa atcagctttg tgtatcagga tatcagcagc catgagtgtc ggataggtaa
    10561 gcagtccggc atttacattt tgaggttgct gacggatttt atccttgaag gaggtacatc
    10621 tttcaagttc gcccacataa gcgttcatat tgaggtataa ataaagttcg atcacctctg
    10681 gcacatcgct ttgaatgtat aaggttgctt tttcgggatc gagaccggct gccaggtatt
    10741 gtgccagcac acttttcaca tttccctgta tattttgagg ggttggatgt gtggtaagtg
    10801 aatgataatc agcaataaaa aagaagcaat tgtattcgta ctgcatactt acaaaattgc
    10861 ttaatgctcc gaaataattt ccgagatgta aatttcctgt gggcctgatg ccgcttaaaa
    10921 ctgtcttcat tgatcttatt ttaccgccac ggtgtgcaga ttgagatagc ataacctgct
    10981 acgggcttag tgatttattt tttcttccta aaaaagtgtg caaagttaat cattttaatg
    11041 caaaaaagcc acctgtagtg ataagtggct tattgaaata tcggtttttc ccttttttat
    11101 tcaatcggta tggttcgctt aattcgctga tcgagtgatg taagcgtttc ggttgtggct
    11161 acaccgggta tctcctgcag tttgccgttg agcaattcca tcagatgttc atcatttttg
    11221 gcgtacattt ttatcagtat ggtatatgct ccaagtgtgt actgcgactc aaccacttcg
    11281 ggtatttttt ccagttcggc taccacatcc ttgtacatgg atgctctttc gagggtgatg
    11341 cctatataca cacatatctg gaagccgagc attttgggat ttaccgaata acccgatccg
    11401 gtaatcactc ccatatcgat aagacgctgt acgcgctgat ggatggcagc tctggatact
    11461 tcgcattcct cggccacatc cttaaaaggt ttacgggcat tttgggaaat tatccgtaaa
    11521 attttacggt ctaatttatc tattttttcc atagaattaa tcgtttagca ttttgccaac
    11581 aaatatatgt attttttgta ataaacgaaa caaattcact ataataatgc aaattttgaa
    11641 atattttttt gtaaaaacgt ctgtttcgat atgtttatgt aaaaaaaaca accgaacaac
    11701 ttgtgtccga ttgcttttta gttatcaggt tttaatacct ttctattttt ctattgaaag
    11761 taactcaact tcgaaaacca gggtgctgag tggttcaata cctcttgctc ctttggaacc
    11821 ataagccagg ttttgaggaa tatagaattt atattttgat cctacaggca tgagttgaag
    11881 tccttctgtc caacccggaa taacctgatt aacaggaaac atggcaggtt catttctttt
    11941 aatggagctg tcaaattctg ttccatcgag ca
//
LOCUS       DAOPZK010000059        11909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_6064_length_11909_cov_19.040830, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000059 DAOPZK010000000
VERSION     DAOPZK010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11909
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6064_length_11909_cov_19.040830"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2097)
                     /locus_tag="PKH68_05120"
     CDS             complement(<1..2097)
                     /locus_tag="PKH68_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNX12351.1"
                     /translation="MKKLIFFTVFVLTTIWGYSQQLTSPNGNFQLNFSLNTVGEPVYQ
                     LSYKGKAVILPSKLGFILKNSTPLTSGFILQDKKISSFDETWKPVWGEVSQIRNQYNE
                     LLVTLEQSKQNRYMKIRFRLFDDGLGFRYEFDRQPELSYFQVSDECTEFALSGDHKAF
                     WIPGDYDTNEYFYTTTRLSEVNADRTDGSGIGTHGYFAKNATQTPLMMKTDDGLYINI
                     FEAALLDYSAMNLLIDQKKNVLTSALVPDATGTKVYMQTPHNSPWRTVIVSDKATDIL
                     ASKTIMNLNEPCKIENTDFIKPQKFIGIWWGMHVPDHWTWNYSDTPNLKLKTTDWNAL
                     KPNGKHGATTENVKRYIDFAAANGIQGVLVEGWNIGWEDWSGNWKEEVFDFITPYPDF
                     DVKGLQAYAASKGVKLIMHHETSASATNYERRIDKAIEFMKDNGYDAVKTGYVGWIIP
                     RGEKHDGQWMVNHYQRVIEKMAANRLMIDQHEPVRPTGQHRTYPNLLACEAARGNEFN
                     AWSVGNPPEHETILPFTRLMGGPMDYTPGVFEIKMDYYKPGSAYQVHTTLAKQLALYI
                     TMYSPLQMAADLVENYEKRMDAFQFIKDVAVDWDDTRYLEAEPGDYITIARKAKGTAD
                     WFVGAITDENARMATINFNYLEPKTWYVATIYGDAPTAHWKDNPMAYQIRKVLVQNIS
                     VLKQQLAAGGGVAISIK"
     gene            2527..4107
                     /locus_tag="PKH68_05125"
     CDS             2527..4107
                     /locus_tag="PKH68_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNX12352.1"
                     /translation="MKTNNENRRGSSASGASRDGNSFEKRDVRSILKGKSGNFHSNDN
                     RGDRHPNSGNERNDGFKPRFTDRKFDNRSDKPSYGGNRNSDTYNRPYNSDRKPFVSRE
                     NRNSDDNSRPYNSDRKPFLNKDARNSDGNARPYNSDRKPFLNKDARNSDGNARPYNSD
                     RKPFVSRDNRNSDGNARPYNSDRKPYGRDDRGDDRRFSSDKPRFVRKSETGDRPAYKK
                     PYDNDEPREGKLNLRKAGEVANSGFIETRFDNKSNGVTFRKRNKDDNYDPNAKYSRKK
                     QIEYIKSNVDPNKPVRLNKFLANAGICSRREADEYIQAGVISVNGEIITELGTKILPT
                     DKVMFHNQTVRSERKIYLLLNKPKDCVTTSEDTHAKLTVLDLVKNACSERIYPVGRLD
                     RNTTGVMLLTNDGDLASKLTHPKYNKKKIYHVVLDHDLSKNDFNKILEGIELEDFTIK
                     ADALEFVKEDSMKEVGIEIHTGQNRVVRRIFEKLGYKIVRLDRVYFAGLTKKNVPRGK
                     YRFLTEREVTMLKMGAHE"
     gene            4296..4853
                     /locus_tag="PKH68_05130"
     CDS             4296..4853
                     /locus_tag="PKH68_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12353.1"
                     /translation="MNTGQEQQAEQTTIPVKKVRKEPNRLVKYILNHKLVFSLIFALV
                     VVFLWGQWRIGKLEKQQKALVETYNLKMDSTQLANMMVTSKAFSWAVRSDMLRNNPDQ
                     VQLYLSNIVGEPHIKKAFIVDAEKSLIILSSDKKEEGTPFLDVAAMQTKVTYYQQRDS
                     VVRFISPYMGLNRKAGISVIEADFK"
     gene            5263..>6292
                     /locus_tag="PKH68_05135"
     CDS             5263..>6292
                     /locus_tag="PKH68_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX12354.1"
                     /translation="MLKNHPKGLIVAFFTNMGERFGFYTMMAILVLFLQVKYGLDEKV
                     AGDYYSWFYFAIYALSLLGGILADWKKQYKTVILYGQIIMFVGYVMMAIPSMSLYGTL
                     GALLVISLGNGFFKGNLQAVVGQLYDDPKYAKFRDSAFMVFYMGINVGAFFAPFVATG
                     IRNWWLETNGFIHDGSLPALCHQMINGTLKDPAQFQVLANNAILSKVPVTDLTAFAHQ
                     YLDVFSRGYNWAFAIAAVAMIVSLSVYVIFNKLLPNKSIVKAVDKQTNTIDNQSSHTG
                     RILVVSLFLMAITTILVYLLPVLTSPAESTSKIGLGLALGLFVAFVSYIFQMSTKEER
                     PRVISLILV"
     assembly_gap    6293..6392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6393..7250
                     /locus_tag="PKH68_05140"
     CDS             <6393..7250
                     /locus_tag="PKH68_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX12355.1"
                     /translation="FQMSTKEERPRVISLILVFIVVIFFWMSFHQNGLTLTLFARDYT
                     VKEVGPFTNLFFNLESLLCVIATIAGMVLMFRSKSMMRSRVIGAGLTLAFGAACYYFI
                     SNAKELNPISPEIFQAFNPLFIVALTPVVMGFFSFLSSKGKEPSTPRKIGFGMIIAAS
                     AFIIVLVGSLHLVSPHLLDGAPTPATSRISPYWLMSSYLILTIAELFLSPMGLSFVSK
                     VAPPRFQGLMQGGWLLATAVGNKLLFIGSTLWGRVDLYVLWGVFFICCIISATFIFAM
                     MRRLEKVTK"
     gene            complement(7414..7851)
                     /locus_tag="PKH68_05145"
     CDS             complement(7414..7851)
                     /locus_tag="PKH68_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12356.1"
                     /translation="MKVRIVFYVMVLLTVIGCTQNGAKLPEGTWKMVNFQYYSGDTLK
                     ANDVMDDSVNQVKIWTPNYFTFVGRSKGDSTYQDNYGGGTYKLDGINYEENIQFHSYK
                     SYIGQTVKLLLELKGDTLVQTFPVDNMWKPEKSHFIEKYVRLQ"
     gene            8064..8954
                     /locus_tag="PKH68_05150"
     CDS             8064..8954
                     /locus_tag="PKH68_05150"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNX12357.1"
                     /translation="MIQHIEELIRTRKSIRTFEGTAIEPEIKQQIISYMKTGNVGLFG
                     NKVDFYWIDGSTGEYKDVKLGTYGVINGTKNYIAGKVKEGDKNLEDFGYCMEKVILYC
                     TQLNIGTCWMGGTYNKTDFATAVGLQEDEIIPAVTPVGYFGDRKRTIDKMFRRFAGSD
                     QRKPFDELFFTETFGKPLTTEQREMYGYFLEMVRLAPSASNKQPWRIVLIGNVFHFYL
                     RRTPGYNSKFMSSDLQRVDMGIAMSHLDLALSEKNIKHRWTSSDPGLIMDEMTEYCAG
                     CEVELPLNPLKGTLRIKSKE"
     gene            9150..10118
                     /locus_tag="PKH68_05155"
     CDS             9150..10118
                     /locus_tag="PKH68_05155"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HNX12358.1"
                     /translation="MINTAHAIFHPEQFQGRGKTNRYFEGWYYKVVNAAETDAFAFIP
                     GIAMDANGNQHAFIQVMDGIRRTATYHTFPASDFHPCKGKFEIALGDNLFTDSTLKLD
                     LPGASGRLTFTVQTPWPNPWYSPGIMGPYTFVPFMECYHGILSMDHIINGELTIQGKT
                     IDFTNGRGYMEKDWGQSFPSAYFWMQTNHFSVPGISLKASVANIPWLGSSFTGFIAGI
                     WLHDRLIQFTTYNGTRLLRSFADSRHVQLVMDNKNYRLEILAHREAVTQLASPISGFM
                     TGRIEESMTSRIEVCLIDKKTNTIILKDTGRNAGLEVAGEIKEITI"
     gene            10366..>11909
                     /locus_tag="PKH68_05160"
     CDS             10366..>11909
                     /locus_tag="PKH68_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNX12359.1"
                     /translation="MLTTLGGAGLLPLLAATPNQKPLNIVYIMSDDHTQQVISSYDQR
                     FGITTPNIDRIAREGILFTNSFVANSISGPSRACMLTGKHSHKNGFRNNSDRFDGNQQ
                     TMPKLLQAAGYQTALVGKWHLESNPTGFDYWEILPGQGDYYSPVMYTKDERKTYPGQY
                     VSDLITDKSINWLEHRQKDKPFCLFVHHKAVHRNFMAKSTDLRAFENKTYPYPDNLFD
                     DYKGRQAAAEQEMSLAKDYDLVYDLKMNLTPETKSPLSGMFNPSDSTGTYGRMNALEK
                     AAWNEVYDSIKTDFISRNLKGEELVKWKYQRYIKDYLKTVKAMDDNIGRLLDYLEANN
                     LLENTLIVYTSDQGFYMGEHGWFDKRFMYEESLRTPLVMRLPAKYNAHGKATEMIQNI
                     DHAPTFLEIAGAKVPSDIQGESYLPILKGKHLKKARKALYYHFYEFPAEHMVKRHYGI
                     RTERYKLIHFYNDIDTWELYDLKKDPSEMNNLIENPGYGKKIKQLKKQLWKLQVQYDD
                     PIRMKYP"
BASE COUNT         3532 a         2582 c         2456 g         3239 t
ORIGIN      
        1 cttaatactg atagctactc ccccgcctgc agcaagttgc tgtttcagca ctgaaatgtt
       61 ctgaaccaat actttgcgta tctggtatgc catgggattg tctttccagt gcgctgtggg
      121 agcatctcca tagatggttg ccacatacca ggtttttggt tccaggtagt tgaaattgat
      181 ggttgccata cgcgcatttt catcggtgat agcgccaaca aaccagtcgg cagtaccttt
      241 agccttgcgg gctatggtga tataatcgcc cggttcagct tccaggtagc gggtatcgtc
      301 ccagtctaca gctacatctt tgataaactg aaacgcatcc attctttttt catagttttc
      361 caccaggtcg gcagccatct gcaacggact gtacatggta atatagagtg ccagttgctt
      421 ggccagtgtg gtgtgaacct gataagcact tccgggttta taataatcca tttttatttc
      481 aaatacaccg ggcgtataat ccatcggtcc tcccatcaat cgggtaaaag gaaggatagt
      541 ttcatgttcg ggcggattac ccacgctcca ggcgttgaat tcattgcccc ttgctgcctc
      601 acaagccaac agattgggat aagtgcggtg ctgaccggtg ggacgaacag gttcatgctg
      661 atcaatcata aggcggttgg cagccatttt ttcaatcacg cgctggtagt gatttaccat
      721 ccactggccg tcgtgttttt caccccgtgg tataatccaa cccacatatc ctgttttcac
      781 agcatcgtag ccgttgtctt tcataaattc aatggctttg tcaatgcggc gttcgtagtt
      841 ggtagccgag gccgaagttt cgtggtgcat gatcagtttc acccctttgg aggctgcgta
      901 ggcttgaagt cctttcacat caaaatcggg ataaggagta atgaaatcaa aaacctcttc
      961 tttccagttg cccgaccagt cttcccaacc gatattccag ccttcgacca gcacaccctg
     1021 aatgccgttg gcagcagcaa aatcaatgta tcgtttaaca ttttcggtag tagcaccatg
     1081 tttaccgttg ggtttcaagg cgttccagtc ggtcgttttg agtttcagat tgggcgtatc
     1141 ggagtagttc caggtccagt gatcgggcac gtgcatgccc caccagatac cgataaattt
     1201 ctgtggttta ataaaatccg tattttctat tttgcagggt tcattcaggt tcataattgt
     1261 tttagatgcc agtatgtctg tcgctttatc actcacaatg acggtgcgcc acggcgagtt
     1321 gtgtggtgtc tgcatataca cttttgtgcc ggtagcatcc ggaacgaggg ctgaagtcag
     1381 cacatttttt ttctgatcaa tgagcaaatt catggcagaa taatcgagca gcgctgcttc
     1441 aaaaatattg atatacaggc catcatctgt tttcatcatc aagggtgtct gtgttgcatt
     1501 tttggcaaaa taaccgtgag ttccgatacc cgatccatca gtgcgatcgg cattcacttc
     1561 cgaaagccgg gtggtggtgt agaaatactc attcgtatca taatcgcctg gtatccagaa
     1621 tgctttatga tcgcctgaaa gggcaaattc ggtgcattcg tccgaaacct gaaaataact
     1681 cagttctggc tgccggtcga attcgtagcg aaatcccagt ccgtcatcaa acagccggaa
     1741 acggattttc atgtagcggt tttgtttcga ttgttcgagc gtcaccagca attcgttgta
     1801 ctgatttcgg atctggctta cctcgcccca aaccggtttc caggtttcat cgaacgagct
     1861 gattttttta tcctgtaaaa taaaaccgga agtcaacggg gttgaatttt tcagtataaa
     1921 tccaagtttt gaaggcagaa tcaccgcttt ccccttataa gagagctgat aaacaggttc
     1981 acccacagtg ttcagagaaa aattcagctg gaaattgcca ttgggtgaag taagctgctg
     2041 cgaataaccc caaattgtgg tcagcacaaa tactgtgaaa aaaatcagtt ttttcatgag
     2101 ttgtattttt tttaattaat aattcgaagt tttgtttgac tttttaccat attgaagaaa
     2161 acaaaattag gtaatatatt tcataaaacg ctctcaactt ttagaataag atatgtaatg
     2221 attacgtaaa cgtttgaact atatacagcg tctctgttca gggcatactg aaccccgaaa
     2281 caggactaat cacagttgct taaataatct cgggtaacac atcatttagt aataagtcat
     2341 caaccatacc gacaccttta ttatattaaa aatctgattg ctgaacaccg ataatcttcc
     2401 tgtaagtatt acattaaccc cctgtttatt ccttaaccct taaattttat tataccgact
     2461 ataaaatgtg ctgaaaatcc atatttttta ccgacctttg caaaaatatt atttttcaat
     2521 aaactaatga aaacaaacaa tgaaaatcgg cgaggaagta gcgcttccgg agccagccgt
     2581 gacggtaatt cgttcgaaaa acgtgatgta agatcaatcc ttaaaggaaa aagtggaaac
     2641 ttccacagta atgacaaccg cggtgacaga cacccaaact ccggtaatga aagaaacgat
     2701 gggttcaaac cgcgttttac tgacaggaaa tttgacaaca gaagtgataa accctcatac
     2761 ggaggcaaca gaaactccga tacgtataac agaccctata attccgaccg caaaccattc
     2821 gtaagccgcg aaaaccgcaa ttcggatgac aattcaagac cctacaactc tgaccgtaaa
     2881 ccatttctta ataaagatgc gcgcaattca gacggcaatg ccagacctta taattcagac
     2941 cgtaaaccgt ttctgaacaa ggatgcgcga aattcggacg gaaatgcaag accttacaac
     3001 tctgaccgta aaccattcgt aagccgtgac aaccgaaatt cagacggaaa tgcccggccc
     3061 tataattctg accgtaaacc ctatggtcgt gacgacagag gagatgatcg ccgtttcagt
     3121 tcagataagc cacgatttgt aagaaaaagc gaaacaggcg acagacctgc ctacaaaaaa
     3181 ccttacgata atgatgaacc ccgcgaagga aaactgaatt taagaaaagc cggtgaagtt
     3241 gccaactcag gctttattga aacacgtttc gacaataaat ccaacggagt aactttccgc
     3301 aaacgaaaca aagacgacaa ctacgatccc aatgccaaat acagcaggaa aaaacagatt
     3361 gaatacatta aatcaaatgt tgatcccaat aaacctgttc gtctgaacaa atttctggct
     3421 aatgccggca tttgctcccg tcgtgaagct gacgaataca tacaggctgg tgtcatttcg
     3481 gttaatggtg aaatcatcac agaacttggc acaaaaatac tgcctaccga taaagtgatg
     3541 tttcacaatc aaacggtacg ctcggagcgc aaaatttacc tgttgctcaa taagcccaaa
     3601 gactgtgtaa ccacctcgga agatacacac gccaaactga cagtacttga tctggttaaa
     3661 aatgcctgca gcgaacgtat ttaccccgta ggacgcctcg accgcaacac taccggtgta
     3721 atgctgctta ccaacgacgg cgacctggct tcgaaactta cccatccgaa gtacaacaaa
     3781 aagaaaatct atcacgtggt acttgatcat gatctgtcaa aaaatgattt caacaaaatt
     3841 ctcgaaggca ttgaactgga agattttacc atcaaagccg atgcccttga atttgtaaaa
     3901 gaagacagca tgaaggaggt ggggattgag atccataccg gtcagaacag agtggtacgt
     3961 cgtattttcg aaaagctggg atacaagatt gttcgtctgg atcgggtcta ttttgccggt
     4021 cttaccaaga aaaatgtgcc cagaggcaaa taccgcttcc tcaccgaaag agaggttact
     4081 atgctcaaaa tgggtgcaca cgagtgattt ggatgttggc tattaagtta ttaagttatt
     4141 ggttattgcc tctgtgcatt tttcgaaaca aattagtgaa atgcctccgg ggagtcaaat
     4201 tattacagtt catatttgtg tatatttcgt ttgaaaatta acaaattaac ttttcggatt
     4261 aaattttgat aatgtaacct ttaatataat ggactatgaa caccggacag gaacaacaag
     4321 ctgaacaaac caccatcccc gtaaaaaagg taagaaaaga gcctaacaga ttggtaaagt
     4381 atatacttaa ccataaactg gtattctcac tgatttttgc attggtcgtc gtatttctgt
     4441 ggggacagtg gcgaatcggt aaacttgaga aacagcaaaa agcgcttgtt gaaacctata
     4501 atctgaaaat ggacagtacg caacttgcca atatgatggt tacttccaaa gccttttcgt
     4561 gggcagtgcg aagcgatatg ctccgcaata accccgacca ggtgcaattg tatctgagca
     4621 acatcgtcgg cgaaccacac atcaaaaaag cattcatagt ggatgccgaa aaaagcctca
     4681 tcatcctctc atccgacaaa aaggaagaag gaactccatt tcttgatgta gcggccatgc
     4741 agacaaaagt tacttattat cagcaaagag acagcgtggt acgttttatt tcaccctaca
     4801 tgggactgaa ccgcaaagcc ggaatttcgg tcattgaagc cgatttcaaa taaagcaaca
     4861 atataataaa tctaaattgt aaagggaact gcaaggttcc ctttattttt tgtaatatcc
     4921 aattctacca taaccaagtc ccggttataa ctgataattt gctaaaagtg acataatgac
     4981 attattaaca gacagttagt tgattggcgc ggatctgata acaaatctat ggattaacca
     5041 cagatacagc acactgtata attaaataga acacggatat atctaaataa tacgtaacaa
     5101 caggtctccg attctgtcac acgggaggag aaaatgcatt tccgttgcat ttccttacag
     5161 taattcattt tgaaaattct ttcatttaaa tctttatgat tatatttgca atccgattaa
     5221 aataatgtga attttttcat taactaataa agataaacga atatgttaaa aaaccacccg
     5281 aagggtttaa ttgtagcatt ttttaccaac atgggcgagc gattcggctt ctataccatg
     5341 atggcgattt tggtgctttt tctgcaggta aaatatggcc tcgacgaaaa agtagccgga
     5401 gattattaca gttggttcta ttttgccatt tatgccttat ccctgttggg tggtatactg
     5461 gccgactgga aaaaacaata taaaacagta attctctacg gacagattat catgtttgta
     5521 gggtatgtaa tgatggctat tccctccatg tcgctctacg gcactctggg agcattgctt
     5581 gtgatttcat taggcaatgg ttttttcaaa ggaaaccttc aggcggtagt aggtcagctt
     5641 tacgacgacc cgaagtatgc caaattccgt gattcggcct ttatggtttt ctacatgggt
     5701 atcaatgttg gtgctttctt tgcaccattc gtagctaccg gtattcgcaa ctggtggcta
     5761 gaaaccaatg gttttattca cgacggtagt ttgcctgccc tttgtcatca gatgatcaac
     5821 ggtacactca aggacccggc tcagtttcag gtgctggcaa ataatgccat cctgagcaaa
     5881 gttccggtaa ctgatttgac tgcttttgca caccaatacc tggatgtgtt ctcccggggt
     5941 tataactggg catttgcaat tgcagcggtg gctatgatag tttcgttgtc agtgtatgtt
     6001 attttcaaca aactgctgcc taataaatca atagtgaaag ctgtggataa acaaaccaat
     6061 accattgaca atcaatcatc acataccggt cgtattctgg ttgtatcttt atttctgatg
     6121 gctattacta ccattctggt gtatctgtta cccgtattga cttcacccgc cgaatccacg
     6181 tcaaaaattg gattaggact tgcgcttgga ctattcgtgg ctttcgtttc ctacatattc
     6241 caaatgtcga ccaaggaaga acgcccccgg gttatttcac tcatacttgt atnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttccaaat gtcgaccaag gaagaacgcc
     6421 cccgggttat ttcactcata cttgtattta ttgtggttat attcttctgg atgtcgttcc
     6481 atcagaatgg gctgacactc acactctttg cgcgtgacta caccgtcaag gaagtgggac
     6541 cattcaccaa cctgttcttc aacctggaat cactcttgtg tgtgattgct accattgcgg
     6601 gtatggttct tatgttccga agcaaatcca tgatgcgttc gcgcgtaatc ggtgccggtt
     6661 taacccttgc attcggagca gcctgctact attttatcag caatgccaaa gaacttaatc
     6721 ctatctctcc cgaaattttc caggcgttca atccgctgtt tatcgtggca cttacaccgg
     6781 tggtgatggg cttcttcagt ttcctaagtt caaaaggcaa agagccttcc acacctcgta
     6841 aaattggatt tggtatgata atagctgctt cagcatttat catcgtactg gtaggatcgt
     6901 tgcacctcgt atcgcctcac ttactggatg gagctcccac cccggccact tcgcgtatat
     6961 caccgtactg gcttatgagc agctacctga tattgaccat tgccgaactt ttcctcagtc
     7021 cgatgggtct ctctttcgtt tcgaaagtgg ctccacctcg cttccagggt ctgatgcagg
     7081 gcggctggct gctggctact gctgtgggta ataaactgct ctttatcggc agcacgctct
     7141 ggggacgggt cgatctgtat gtactgtggg gagtattctt catttgctgc attatctccg
     7201 ctacgtttat attcgctatg atgcgaaggc tggaaaaggt aacgaaataa gaagacaagc
     7261 cccctaaatc ccccgaaggg ggagcaagaa aagatagtat ttgggtacta aacttacata
     7321 aaaaaatcct gcaatcgttg attgcgggat tttttttatt gacgtttttc gtttcgtgaa
     7381 gcgttgaacg tttaccgtta accgtttgag ggtctattgc aaccgtacgt atttttcaat
     7441 aaaatgtgat ttttcaggtt tccacatatt atctaccggg aaagtctgaa caagggtatc
     7501 acctttcagc tccaatagta atttcaccgt ttgaccaata taagatttat aactatgaaa
     7561 ttgaatgttt tcttcatagt tgatgccatc caacttatag gttccacctc cataattgtc
     7621 ctggtaggtt gaatcgccct tacttctacc aacaaatgta aaataattgg gtgtccagat
     7681 cttaacctga ttaactgaat catccattac atcattagcc tttagtgtat cccccgaata
     7741 atattgaaaa ttaaccatct tccaggttcc ttcgggcaat ttagcgccat tttgagtaca
     7801 gccaataacg gttaataaaa ccattacata aaacacaatt ctgactttca taattaataa
     7861 ttttttaatt gattagttta ctgcaattta caacatatta atttataatt attttaaata
     7921 aaacaatttg aactattatt aagttcaatg caaaaaaatg aaagtagaaa tctacagttc
     7981 actgtttgac gaatatcaaa tacgttgtcc gttttgttgc tttttttctt agctttgtgt
     8041 ctgaaacaca acaaaattat aatatgatac aacacataga agaactaata cggacgcgca
     8101 aatcgatacg aaccttcgag ggtacagcca tagaaccgga aataaagcaa cagattatca
     8161 gctatatgaa aaccggcaac gtggggctat tcggcaataa ggtggatttc tactggatag
     8221 acggcagtac cggtgaatat aaagatgtta agctcggcac atatggagta atcaacggca
     8281 caaagaatta tatagccgga aaggtaaagg aaggagacaa aaacctggaa gacttcgggt
     8341 attgtatgga aaaagtgata ctttattgca cacaactgaa catcggcacc tgctggatgg
     8401 ggggtacgta caataaaacg gactttgcaa cggctgtggg gttgcaggag gatgaaatta
     8461 ttcccgccgt cacacctgta ggctatttcg gggacagaaa acgaaccatc gataagatgt
     8521 tccggcgctt tgcaggttcg gatcagcgca agcccttcga cgaattattt ttcacagaaa
     8581 cctttgggaa gccgcttaca acggaacaac gggaaatgta tggttatttc ctggaaatgg
     8641 tacggctggc accttccgct tccaacaaac agccctggag aatagtattg ataggtaatg
     8701 tgtttcattt ctacctccgg cgtacacctg gttacaacag caaattcatg tcgtccgacc
     8761 tgcaacgtgt ggatatgggc atagctatgt ctcatcttga tctggcttta tccgaaaaga
     8821 acataaaaca tcgctggaca agttccgatc cgggattgat aatggatgag atgacagaat
     8881 actgtgcagg gtgtgaggta gaactgcccc taaatcccct aaaggggact ttaagaataa
     8941 agagcaaaga ataaaggttg aaagaataaa gacagaaaga acagagacca atagaaccca
     9001 gacggctact aaatccccta aaggggactt aaagaaaaga agcaacccca gatggtctac
     9061 acatccgctt tagtacctgt ccctaacttg tttagggagg cttggggcag ttttattaaa
     9121 gtataattca tgatttatac caaaatatta tgatcaacac cgcacacgct atatttcatc
     9181 ccgagcaatt tcagggacgg ggaaaaacca accgatactt tgagggttgg tattacaaag
     9241 tggtgaatgc cgccgaaacg gatgcattcg cctttattcc cggcattgcc atggatgcaa
     9301 acggcaatca gcatgccttt attcaggtga tggatggaat acggcgaaca gcaacatatc
     9361 atactttccc agcatccgac ttccatccct gcaaaggaaa atttgaaatc gctctgggtg
     9421 ataatttatt tactgactca acactgaaac tcgacctgcc gggtgcttca ggcaggttaa
     9481 cctttacagt acaaacaccc tggccaaatc cgtggtattc gcccggcatc atgggcccat
     9541 acacgtttgt tccctttatg gaatgctatc acggaattct gagcatggat cacatcatta
     9601 atggtgagct cactatacag ggcaaaacca tcgacttcac caacggacgt ggctacatgg
     9661 aaaaagactg gggacaatcg ttcccatccg cctacttttg gatgcagacc aaccatttca
     9721 gtgtaccggg aatttcgctg aaggcatcgg tggccaatat cccctggctg ggcagttcat
     9781 ttaccggatt tatagcgggt atctggctcc acgacaggtt gatacaattc actacctaca
     9841 acggtacccg gctactcagg tcctttgcag acagccgcca tgtacaactc gttatggaca
     9901 ataaaaacta ccggctggaa atactggctc atcgggaagc tgtaactcag ctcgcctcac
     9961 ccatttcggg atttatgact ggaagaatag aagaaagcat gacctcgcgc atcgaggtgt
    10021 gcctcatcga caaaaagact aacaccatta tcctgaaaga taccggccgt aatgctggcc
    10081 ttgaagtggc aggagaaata aaggaaataa ctatttagtg aattgagaaa aagaacagat
    10141 cttaaaaaag ttggattatc acagttgaat ggcgcattac ttttaatcgg atttttttta
    10201 ttatttttct aaaaaccatt acattgctaa aataatttgt ttaataatag tatctttgtg
    10261 ggtgaaaaca tcccgtaaca tcgggtttga atcaatacaa aatcacacta ttcgtcacac
    10321 cttaactcaa ttctgatgtc taaaatcaac attccctcca gagccatgct caccacgctg
    10381 ggtggagcag gtttactgcc actactggct gcaactccca accagaaacc tctgaatata
    10441 gtttatatca tgtccgatga ccatacacag caggtaatta gcagctacga tcagcggttt
    10501 ggtatcacca cacccaatat cgaccggatt gcccgcgaag gcatactctt caccaatagt
    10561 tttgtggcca attcaatctc cgggcccagc cgcgcctgta tgctcaccgg taaacatagt
    10621 cacaagaatg gtttcaggaa taacagcgac cgttttgacg gaaaccagca aaccatgcct
    10681 aaactgcttc aggctgccgg ttatcagacg gcattggttg gaaaatggca tttggaaagc
    10741 aatcccaccg gattcgatta ctgggaaata cttcccggac agggtgatta ctatagtccg
    10801 gtgatgtaca ctaaagatga aaggaaaacc tatccgggtc agtatgtgag cgatctgatt
    10861 accgataaaa gtatcaactg gctggagcac cgacagaaag acaagccttt ttgtctcttt
    10921 gtacatcaca aagctgtaca tcgtaacttt atggccaagt ctacagacct gagagcattc
    10981 gagaataaaa cttatcccta tcccgacaat ctgtttgatg attataaagg ccgtcaggct
    11041 gcagctgaac aggaaatgtc actggctaaa gactatgatt tagtgtacga cctgaaaatg
    11101 aacctgaccc ccgaaacaaa atcgccactc agcggaatgt tcaaccccag cgattcgacg
    11161 ggcacctatg gccggatgaa tgcccttgaa aaagccgcct ggaacgaagt ctatgattcc
    11221 ataaaaacag atttcatttc gcgtaacctg aagggtgagg aactggtaaa atggaaatat
    11281 cagcgatata taaaggatta cctgaaaacc gtgaaagcaa tggacgacaa catcggtcgt
    11341 ttactggact acctggaggc aaataacctg cttgaaaata cgctcatcgt ttacacttcc
    11401 gatcagggat tttatatggg cgaacatggc tggttcgaca aacggtttat gtatgaagaa
    11461 tcgcttcgaa caccattagt catgcgttta cccgcaaaat acaatgccca cggaaaagca
    11521 acagagatga ttcagaatat tgaccacgct cctactttcc tcgaaattgc cggtgcaaaa
    11581 gttccatcgg atatacaggg cgaatcctat ctgccaatac ttaaaggaaa acacctgaaa
    11641 aaagccagaa aagcgctgta ttatcatttc tacgaatttc cggctgaaca tatggtcaaa
    11701 aggcattatg gcatccgaac tgaacgatat aaattaattc atttttacaa cgatattgat
    11761 acctgggaac tctacgatct gaaaaaagat ccttcggaaa tgaataacct gatagaaaat
    11821 ccgggatatg gaaaaaaaat taagcagctg aaaaaacaac tctggaagtt gcaggtacag
    11881 tatgatgacc ctatccgtat gaaatatcc
//
LOCUS       DAOPZK010000060        11772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_6188_length_11772_cov_24.411453, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000060 DAOPZK010000000
VERSION     DAOPZK010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6188_length_11772_cov_24.411453"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1225)
                     /gene="cydB"
                     /locus_tag="PKH68_05165"
     CDS             complement(65..1225)
                     /gene="cydB"
                     /locus_tag="PKH68_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNX12360.1"
                     /translation="MITYSFLQHYWWFLISLLAGILSFLLFVQGGQSMIFSLAKKEDE
                     RRLLVNVLGKKWEYTFTTLVTFGGAFFASFPLFYSTSFGGAYWVWMLILFCFTIQAVS
                     YEYQNKPGRFFSTNTYRSFLFINGLLGTFLLGAAIATFFTGSEFSVNKQNIAEFGSQL
                     SPVISQWENPLHGLEALFNVRNWLFGLAVFFLARTLAVLFFKNRLNNAEIQSRLQRAL
                     VMNAVPFLVFFLAFVFWTLFANGFAVKPGTNEVVLEPMKYMNNLIEMPVLLLLFLIGV
                     VLVLYGIIKSYLNDSWRKGIWFAGAGTIITVTTLFLVAGYNNTAYYPSTVDMQSSLTI
                     FNSSSSFFTLKIMSVVSLLIPFIAAYIFFAWRALEKTHTSIEEINEKDSHAY"
     gene            complement(1297..2847)
                     /locus_tag="PKH68_05170"
     CDS             complement(1297..2847)
                     /locus_tag="PKH68_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNX12361.1"
                     /translation="MELLDVSLVNWSRAQFALTAMYHWLFVPLTLGLGIIQAIMETIY
                     VKTGKEEWKKTAKFWMTIFGINFAIGVATGIILEFQFGTNWSNYSWFVGDIFGAPLAI
                     EGMLAFFMEATFISIMFFGWNKVSKGWHLAATWLTIAGATISALWILVANAWMQNPLG
                     MEFNPTTVRNEMIDFWSILTSPIAINKFLHAVTSGWVLGAIFVAGVSAWYLIKKREVE
                     FAKRSLKVASIFGLVSTLLIAYTGDGSAYHVAQKQPMKLAVMEGLYKGQEGTPLIVIG
                     VVNPAKEKYNDDVEPVLVPLRIPKMLSFLGYRDFNAFVPGIADIIEGGYTTLKDTTKA
                     LSFEQKKTMGQTAITALKQYKEAEKTKDTTAMASHKAVLDANYKYFGYGYLDKPEDSI
                     PNVPLIFYSFHLMVGLGFFFIALFAALLFFDYKKTLDKQKWLLWVTLFTIPLGYLAGE
                     LGWVVAEVGRQPWAIQDVLPVQAAISGIQTSSVQITFFLFLVLFTALLIAEVRIMLKQ
                     IRKGPAEE"
     gene            complement(2952..3197)
                     /locus_tag="PKH68_05175"
     CDS             complement(2952..3197)
                     /locus_tag="PKH68_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HNX12362.1"
                     /translation="MKIPRFLENIYRFYRDGFKNMTVGVTLWTIALIKLFIMFAILRV
                     FFFPNYLNSRFDTEQEKANHVTNELIEKAKTVQPDNN"
     gene            complement(3451..4059)
                     /locus_tag="PKH68_05180"
     CDS             complement(3451..4059)
                     /locus_tag="PKH68_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX12363.1"
                     /translation="MKNLSLILNGVLFIAVIILFFLVLGKGTGKVAGVGVKSGDSTKM
                     VTLPIAYVNIDSLLLNYTFAKNANEALIKKQEDSRLTVNARARQLQTEMNEFQRKLEN
                     NAFLSRERAEAAQTEILKKQKDLQDMDGRLSQQLMEQQQKMSEQLRDTINAFMKDYNK
                     DGKYQMIISNTANDNILYAGKTYDITSDVIKALNARYSKAKK"
     gene            complement(4073..4300)
                     /locus_tag="PKH68_05185"
     CDS             complement(4073..4300)
                     /locus_tag="PKH68_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12364.1"
                     /translation="MLVTLLFTTLIVLISVFLLTFRILLVKGGKFPNIHIGGSKVLRD
                     RGVSCATTQDRNAQKKSGFHFTEIIKDIEEK"
     gene            complement(4519..5094)
                     /locus_tag="PKH68_05190"
     CDS             complement(4519..5094)
                     /locus_tag="PKH68_05190"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNX12365.1"
                     /translation="MTFSLPVLPYAHNALEPVISEETINFHYGKHHQTYVTNLNGLIP
                     GTEFENADLDTIVKKSSGPVFNNAAQIWNHNFYFLSLTPVKGSVPSENLAKAIDAKFG
                     SFDNFKAEFNKAAITVFGSGWAWLVKTADGGVDIVKESNAGNPLTSGLTPLLTFDVWE
                     HAYYIDYRNRRADYVAALWDLVDWNAVSARF"
     gene            5292..5750
                     /locus_tag="PKH68_05195"
     CDS             5292..5750
                     /locus_tag="PKH68_05195"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNX12366.1"
                     /translation="MKKILGMFLMAAIVLAGSTSCKSKQKVTEISGANVEATAKTNTA
                     ATTASTTATAATETEKTRNEKFSLAEGETNIAAFNSKYHVVVGSFSIQQNAKNLQRTL
                     NSEGNNALVVVNEQGMFRVLIASFNEYTQARERINQINTRFPDAWVLVQK"
     gene            5859..6305
                     /gene="folK"
                     /locus_tag="PKH68_05200"
                     /pseudo
     CDS             5859..6305
                     /gene="folK"
                     /locus_tag="PKH68_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
     gene            6336..6725
                     /locus_tag="PKH68_05205"
     CDS             6336..6725
                     /locus_tag="PKH68_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HNX12367.1"
                     /translation="MENQKEGYYTKKFDVPTKRFCQTMDLRNDPQLIAEYVKRHSEQE
                     VWKEIPAGIREVGILEMEIYLLGTRLFMILETPVDFNWDKAFAKLATLPRQAEWEDYM
                     TVFQLAEKGASSDEKWQLMERIFHLYD"
     gene            complement(6726..6974)
                     /locus_tag="PKH68_05210"
     CDS             complement(6726..6974)
                     /locus_tag="PKH68_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12368.1"
                     /translation="MEDNADKLVVAKYYDSVLNAELDMEVLKSNGIESTFTSDMMVGI
                     FPLFDEKERGISLLVFEADLDKALQILDEYHHSMPVNE"
     gene            7064..8044
                     /locus_tag="PKH68_05215"
     CDS             7064..8044
                     /locus_tag="PKH68_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX12369.1"
                     /translation="MRIFSKYKYQIKTVRRKTVLLLLFLPLLLTIFIFRILPIKWVRR
                     FADFGGRMFYKFGKNSRNRALHNLKLIYGNEVTDIQLTEMAKASLTESVKSFFDYMAY
                     SHVKDKKKYFRLVKVTGEEHLKAAYQRGKGVICLVPHMSSWEFAAITPPMLGYSTSAA
                     SKAMKMELLEWLMVKFRTRRGMKNITREGSYQALTEVLKRGECLIIMTDQDTRVKGVF
                     VDFLGKVAYTPLGASRLLADTDAALVPMAMTRREDGDYLFTIYPELPATKTGDQAQDL
                     MTNTINQNKILGEMIRKNPTQWVWMHQRWKTTPESLERYLKARALEKMKK"
     gene            8082..8690
                     /locus_tag="PKH68_05220"
     CDS             8082..8690
                     /locus_tag="PKH68_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HNX12370.1"
                     /translation="MAQEQFVFSKNVIEFVTVAAETCLLLENATELPKPEFIATCTRM
                     LPLLYLKTSLVDISELNTEGDIEHFVTEEDYLFVKEQVETLLGVDDSYLEVFHPDMAY
                     SDTPIAAFVSENLADIYQELKDFAANYRIGETEIMENALQVCLETFAEHWGQKLLNAL
                     RALHAVRYGENFGLEEDDAGLNPKDYRKLDRDSFLKFQTDEE"
     gene            8680..9342
                     /locus_tag="PKH68_05225"
     CDS             8680..9342
                     /locus_tag="PKH68_05225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX12371.1"
                     /translation="MKNNQHMLKSKISKEEVNTLPIAEFKGEIVVVDEPAKVKPALDY
                     LKQQKVVGLDTETKPSFQRGTIHKVSLVQIASEEKCYLFRLNKIGFPNELLQFLMDVN
                     VKKVGLSLRDDFNGLSKRSIIKPRNVVDLQSIAKDYGILELGLQKMYAILFGQKISKS
                     QRLSNWENPELTGQQMTYAATDAWASLQIYLKLVVKDKLSQKEIDKLMSDANSAQLNS
                     AN"
     gene            9359..10537
                     /locus_tag="PKH68_05230"
     CDS             9359..10537
                     /locus_tag="PKH68_05230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNX12372.1"
                     /translation="MITVHLKPKKEESLLRFHPWVFSGAIQHINGKPTEGELVDALDN
                     QGNFLATGHYQIGSIAVRVLSFEKVDIDNNYWQQKISQAYTVRRELGLINPPYNNTYR
                     LIHGEGDSLPGLIADVYDDTAVFQAHSVGMHLSRQAIAEAIVAVVPAVKNVYYKSETT
                     LPFNAPIEPEDGYLIGKAGNAFKAMENGLEFKVDWLKGQKTGFFVDQRENRALLEKYS
                     KGKKVLNMFCYTGGFSVYSLRGGAELVHSVDSSAKAIELTDSNVAINFPDAKNHVSFA
                     EDAFKFLNDKREEYDLIVLDPPAFAKHREAVRNALKGYKRLNRKALEQIKPNSILFTF
                     SCSQVINKDQFRLAVFSAAAEAKRNVRILHQLTQSADHPINIYHPEGEYLKGLVLFVE
                     "
     gene            10756..11349
                     /locus_tag="PKH68_05235"
     CDS             10756..11349
                     /locus_tag="PKH68_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX12373.1"
                     /translation="MKNICQLTDEILVKLYEDGNNQAFEILLSRYKSKVYTYIYLIVR
                     NKELTEDIFQETFIKAIATIQQGRYVETGRFLGWVNRIAHNLIIDHFRREKNENTFSA
                     DGLEYDIINNSKLSEKSVEDTISNEQVLTDVIHLIDYLPQSQQEVVRMRFFEDLSFKE
                     IAERTEVSINTALGRMRYALLNMRRIAHENDIQLELK"
BASE COUNT         3594 a         2447 c         2473 g         3258 t
ORIGIN      
        1 aaaaaaaaag agactgtctt gcttttgaga cagcctctta ttcttttaca actcacctaa
       61 ttttttaata ggcgtgactg tctttttcat ttatttcctc aatactggtg tgtgtctttt
      121 cgagtgcacg ccaggcgaag aaaatgtatg cggcaataaa cggtatgagc aatgatacta
      181 ccgacattat tttcagggtg aaaaagctcg atgaggaatt aaaaatggtg agcgaacttt
      241 gcatatccac cgttgacggg tagtaagcgg tattattgta acctgccacc agaaacaagg
      301 tggtaaccgt gatgatggtt cccgcaccgg caaaccagat acctttgcgc cagctgtcgt
      361 tcaggtagga tttaataatt ccataaagca ctaataccac tcctatcagg aaaagcagca
      421 gcaacacggg catctctatc agattgttca tgtatttcat gggttccagt accacctcgt
      481 ttgttccggg ttttactgca aatccgttgg caaagagcgt ccagaataca aaggccagaa
      541 agaacaccag aaatggaacg gcattcatca ccagtgcccg ttgcaggcga ctttgaattt
      601 cggcattatt caggcggttt ttgaagaaca atactgccag tgtacgcgcc agaaagaaca
      661 cagccagacc aaacaaccag ttgcgtacat tgaacaatgc ttcaagacca tgcagcgggt
      721 tttcccactg actaatgacg ggcgaaagct gactgccaaa ttcggcgatg ttttgcttgt
      781 tgacactgaa ttcagatccg gtaaagaatg tagcaatggc agcgcccagc agaaatgttc
      841 cgagcaatcc gttgataaac aggaaactgc gataggtatt ggtactgaaa aagcgaccgg
      901 gtttattctg atactcgtaa gatacggcct gaatggtgaa gcagaaaagt atcagcatcc
      961 atacccagta tgcaccaccg aaactggtgg agtaaaacag cgggaaggag gcaaagaatg
     1021 caccaccgaa tgttaccagt gtggtgaagg tatattccca ttttttgccg agcacgttga
     1081 caagcaggcg gcgttcatct tcttttttag ccagcgaaaa aatcatggat tgtcctccct
     1141 gaacaaaaag cagaaaagaa agaattccgg ccagcagcga aatcaaaaac caccagtagt
     1201 gttgtagaaa tgaatatgtt atcataatag attgttttta atattaatga atgatttatt
     1261 ctcctgagag agaaattatt aatatacaaa cggccgctat tcttcagccg gacctttcct
     1321 gatttgtttc aacatgatgc gcacttcggc tatcagcaga gcagtgaata atacgaggaa
     1381 caggaagaaa gtgatctgca cagacgaggt ctgaatgcca gatatggctg cctgcacggg
     1441 caatacatcc tgtatggccc agggctgacg tcccacttcg gccactaccc aacccagttc
     1501 accagccaga taacccagcg gaatagtaaa cagtgtcacc cataataacc atttctgttt
     1561 gtcgagtgtt tttttgtaat cgaagaaaag cagtgcagca aacagggcga tgaagaagaa
     1621 acccaacccc accatcagat ggaatgaata gaatataagc ggtacgttgg ggattgagtc
     1681 ttcgggttta tccagatatc cgtatccgaa gtatttgtaa ttggcatcca atactgcttt
     1741 gtgcgatgcc atggcggtag tgtcctttgt tttttctgct tccttgtatt gtttcaaagc
     1801 tgtgattgcc gtctgaccca ttgttttctt ttgttcgaaa gagagtgctt tcgtagtatc
     1861 tttgagcgtt gtatatcctc cctcaatgat atcagcaata cccggaacga aagcgttgaa
     1921 atcgcggtaa ccgagaaatg aaagcatctt aggaattcgg aggggaacca gaacgggctc
     1981 aacgtcatca ttgtattttt ctttggcagg attgaccaca cctattacta taagcggcgt
     2041 accttcctga cccttgtata atccttccat tacggccagt ttcatgggtt gcttctgagc
     2101 tacatgataa gccgaaccgt cgccggtgta tgctattagc agtgtggaaa ccaatccgaa
     2161 aattgaagcc actttaaggc ttcgttttgc aaattccact tcccgctttt tgatcagata
     2221 ccaggcactc acacctgcta cgaagattgc tcccaacacc cagcctgatg taacagcatg
     2281 caggaacttg ttgatagcaa tgggcgatgt gagaatgctc caaaaatcaa tcatctcatt
     2341 tcgcacggta gtaggattga attccattcc cagcggattt tgcatccagg cattggctac
     2401 cagtatccag agagccgaaa tagttgctcc tgctatggta agccacgtag cagccaggtg
     2461 ccagcctttg cttaccttgt tccatccgaa aaacatgatg gaaataaaag tagcttccat
     2521 gaagaaagca agcatacctt caatggccag cggcgctccg aaaatatcac ctacaaacca
     2581 gctgtaattg gaccagttgg taccaaactg aaattcgagg ataatacccg tggcaacgcc
     2641 gatggcgaag ttaattccga aaatggtcat ccagaatttt gccgtctttt tccattcttc
     2701 tttacctgtt ttcacataga tggtttccat gatggcctgt atgatgccca gcccgagggt
     2761 cagcggcaca aaaagccagt ggtacattgc tgtaagggca aattgtgccc tcgaccagtt
     2821 tactaacgat acatctaata attccataaa taataaagta ttaaatttta tatatgtaag
     2881 gataatttat cttcgtttga aaaagctcat ctcatcctgt tgctgcaggg agaaggcagc
     2941 agcctttcgg ctcagttgtt atcgggttgt acggttttgg ccttttcaat cagttcattt
     3001 gttacatgat tggctttttc ctgttcggta tcaaaccggc tattgagata attgggaaaa
     3061 aagaacaccc tgagtatggc aaacattata aacagtttta tcagtgcaat tgtccacaga
     3121 gtaaccccga cggtcatatt tttgaatcca tcgcgataaa aacggtaaat attttcgagg
     3181 aaacggggaa ttttcattat cgttgacagt ggtggtgtga atactcaaaa aacaaaaata
     3241 cacatttttt tcaatgcagt gattattttg taataatatt attaatttaa caaaaaaaaa
     3301 ccggaataag tgatgtgatt aaagttgaaa aaatggtgat acggagggtc tgcgataagt
     3361 ggaatatcac ccaaatcggt acaaaaaaaa acggaacccc gctttcacaa gcagtgttcc
     3421 gtttccaagt attttcgatt ttgaattgag ttattttttt gcttttgaat atctggcatt
     3481 gagtgcttta attacatcag aggtaatatc gtaggtttta ccggcgtaaa gtatattgtc
     3541 attggcagtg ttgctgataa tcatctgata tttaccatct ttattgtagt ctttcatgaa
     3601 tgcattgatg gtgtcgcgca actgttcgct cattttttgc tgttgttcca tcagttgctg
     3661 cgatagtctg ccatccatat cctgcaggtc tttctgtttt tttagaattt ctgtttgggc
     3721 tgcttcggct ctttcacggc tcaggaatgc gttgttttcg agtttacgct gaaattcgtt
     3781 catttcggtt tgtaattgac gggcacgggc attaaccgtt aatctgctat cttcctgttt
     3841 tttgatcagc gcttcgttgg cattttttgc aaatgtataa ttgagcagca aggagtcaat
     3901 attaacataa gcaatgggta aagtgaccat tttggtcgaa tctcccgatt tcacaccaac
     3961 ccctgccacc ttgccggtgc ctttgccgag tacgagaaaa aacagaataa taaccgcaat
     4021 gaataaaaca ccattcagaa tgagggatag atttttcatg ttcaaatttt aattattttt
     4081 cttcaatatc tttaataatt tctgtaaaat gaaatccgga ttttttttgt gcgttacggt
     4141 cctgagtagt ggcacaactc actccccggt cgcgcaggac tttgctgccg ccaatatgaa
     4201 tgttgggaaa tttacctccc tttacaagca gtattctgaa cgtaagtaaa aatactgata
     4261 tcagaacgat aagggtggta aataacagag taacgagaat attcatagtt tatagctgta
     4321 agagaatgtg caaagatagt ttttttgatg catttcaaat atatgatggg agatttttta
     4381 gaaaattgaa tgtttcgatt tttattttaa cattcatttt gctattagat tctcattttt
     4441 ctgtttattc taatattata aaaaaaagcc gaaaaactgt tagagtttcc ggcttttatt
     4501 agtttgagtt gctttgtatc agaaacgtgc tgaaacagca ttccagtcca ccaaatccca
     4561 cagagcagct acataatcgg cacgacgatt gcgataatca atgtagtatg cgtgttccca
     4621 aacatcaaaa gtgagaagtg gtgtaagtcc gctggtgagg ggatttccgg cattgctttc
     4681 tttcacaatg tcaactccgc catcggcagt ttttaccagc caggcccatc ccgaaccgaa
     4741 tacggtgata gcagctttgt taaattcagc cttgaagtta tcgaaggagc caaacttagc
     4801 atcaatagct tttgccagat tttcagaagg cacacttccc ttcacgggtg tgagcgatag
     4861 aaaatagaaa ttgtgattcc agatttgagc agcattgttg aataccggtc cgcttgattt
     4921 cttcacgatt gtatcgaggt ctgcattctc aaattcggtt ccgggtatta atccgttcag
     4981 gttagttaca tacgtttgat gatgtttgcc atagtgaaaa ttaatggttt cttcactgat
     5041 aacaggttcc agcgcattgt gcgcataagg taatacaggc aatgagaatg tcatagttct
     5101 tttattttta taatgttagt attattaatg cagaaattgc agatgtagaa cggtaaaagc
     5161 cctctttttg tttatagcaa aattgtaaat aatgtaaaaa acaaatcaaa tagttggtta
     5221 acattaattt tcttagcatc tttgcactac aaaattagta aaaaattagt aaaaacataa
     5281 attataagaa aatgaaaaag attcttggaa tgtttctgat ggctgccatt gtattagcag
     5341 gttccacatc atgtaaatcg aaacaaaaag ttaccgaaat ttcaggtgcc aatgtcgaag
     5401 ctactgcaaa gactaacaca gctgccacta cagcatccac tacagctaca gccgccaccg
     5461 aaaccgaaaa aacccgaaac gaaaaattca gcctggccga aggcgaaacc aatattgccg
     5521 ctttcaacag caaatatcac gtggtagtag gtagtttttc tattcagcaa aatgctaaaa
     5581 acctgcaacg taccctgaac agcgaaggaa acaacgcact ggtggtagtg aacgaacagg
     5641 gaatgtttcg cgtactgatt gcttcgttca atgaatacac acaagcgcgt gaacgaatca
     5701 atcagatcaa cacccgcttc cccgatgcct gggtgctcgt acagaaataa ataatttctt
     5761 actgaaattt acaatagaaa cgactttgtc aactgcaagg tcgttttgct tttatatcct
     5821 ttcctgttca aaaaaacact acatttgcaa taaaaaaaat ggcttttgca tttatcggtc
     5881 tgggaacaaa tctgggtgat aagacccgga atataaacaa ggcgatacag ctcataggaa
     5941 tggaagccgg tgatgtggta aacatttctc cgctttactg ttcaaaaccc tggggatttg
     6001 catcggaaaa cgattttgtg aatgcggtta tccttatcga cacaaaactc gatcccctgg
     6061 cattactcga aaaaatcact taccttgaac ggaaaatggg gcgaactgcc agaatcgaag
     6121 gagagtattc cgaccgtatc attgacatgg atatcttatt gtacgaaaat cggattatta
     6181 atcttccgaa gttaagcata ccgcatccac tgcttcataa gcgggatttc gtactggtgc
     6241 cactggctga aatagctccg gatatgattc atccggtgct gaatgaaacg atcaaacgac
     6301 tgctgaacaa actcacaaca acaaatatac ataagatgga aaatcaaaaa gaaggctact
     6361 ataccaaaaa attcgatgtg cctaccaaac ggttttgcca gacgatggat ctgaggaacg
     6421 atccccaact gatagccgaa tatgtaaaac gacacagtga acaggaagta tggaaagaaa
     6481 taccggccgg catccgggaa gtgggcatac tggagatgga aatttacctg ctgggtacgc
     6541 ggctgttcat gatactcgaa acccctgtgg attttaattg ggataaagct tttgcaaaat
     6601 tagccacact tccccgtcag gctgaatggg aagattacat gaccgtattt caactggctg
     6661 aaaagggggc tagttccgat gaaaaatggc aactgatgga gagaatattt cacttgtacg
     6721 actgattatt cgttcaccgg catcgaatgg tggtattcgt ccaggatttg gagcgcttta
     6781 tccagatcgg cttcgaatac caacaggctg atacctcttt ccttctcatc gaaaagagga
     6841 aaaatcccaa ccatcatatc gctcgtgaat gtactttcaa taccgttgct ttttagcact
     6901 tccatatcga gttctgcatt gagcaccgaa tcgtaatatt tggcgactac aagtttatcc
     6961 gcattatctt ccatgagtag ttgttttttc aggtgggtta atgttttgct ttttgctaat
     7021 ataacgcagt ttgacccgtt attgtttcat taaatattaa caaatgcgta ttttttcaaa
     7081 atataaatat caaatcaaga ccgttcgccg caaaacggtg ttactgctgc ttttcctgcc
     7141 attgttgctc accatcttta ttttcagaat tctaccaata aaatgggttc gccggtttgc
     7201 tgattttggt ggcaggatgt tttataaatt tggaaaaaat tctcgtaaca gggcgctgca
     7261 caatctcaaa ttgatttacg gtaacgaagt cacggacatc caattaactg aaatggcaaa
     7321 agcttccctg accgaatcgg tcaagtcatt tttcgattac atggcttatt cgcatgtgaa
     7381 agacaaaaag aagtatttca gactggtgaa agtgacgggt gaagagcatt tgaaagcagc
     7441 ctaccaacgc ggtaagggtg taatctgcct tgtgccgcac atgagctcct gggaatttgc
     7501 tgccatcacg cctcccatgc tcggttactc cacttcggct gccagcaaag ccatgaaaat
     7561 ggagttactc gaatggctga tggtgaaatt tcgcacccgg cggggcatga agaatattac
     7621 ccgtgaaggt tcttatcagg cgctgacaga ggtattgaaa cgtggcgaat gtcttatcat
     7681 tatgaccgat caggacaccc gcgtaaaagg tgttttcgtc gattttttgg gaaaagttgc
     7741 atatactcct ctgggagcct cccgcttact ggccgatacc gatgccgccc tggtgcccat
     7801 ggcaatgact cgccgcgaag acggggatta cctttttaca atctaccccg aactccctgc
     7861 tacgaaaacg ggtgatcagg ctcaggatct gatgacgaac acaattaatc agaataaaat
     7921 actgggagaa atgatccgga aaaatcccac acaatgggtt tggatgcatc agcgctggaa
     7981 aacaaccccc gaatcgctcg aacgctacct gaaagcaaga gcacttgaaa aaatgaaaaa
     8041 gtagatattt tttgctactt ttgcaatatt aaaaatagaa catggcacaa gaacaatttg
     8101 tattctcaaa aaacgtcatc gaattcgtaa cagtggcagc cgagacctgc ctattgctgg
     8161 aaaatgcaac tgaactgcca aagccggaat tcattgctac ttgcaccagg atgctgcccc
     8221 tgctttacct caaaacatcc ctggttgaca tctcagaatt gaataccgaa ggagatatcg
     8281 aacatttcgt taccgaagag gattacctct ttgtgaagga acaagttgaa actttactcg
     8341 gagtggatga cagctatctg gaggttttcc atcctgacat ggcttacagc gacacaccga
     8401 tagcggcatt cgtatccgaa aatttagccg atatctatca ggaactcaaa gattttgcgg
     8461 ccaattaccg gattggcgaa actgaaatca tggaaaatgc cttgcaggtt tgcctggaga
     8521 ctttcgctga acattgggga caaaaactgc tgaatgcgct ccgtgccctt catgccgtgc
     8581 gttacggcga aaatttcggt ctcgaagagg atgacgcagg attgaacccg aaagattacc
     8641 gaaaactcga ccgcgatagc ttcctgaaat ttcaaacaga tgaagaataa tcaacacatg
     8701 ctgaagtcaa aaataagcaa agaggaagtc aacaccctgc ccatagccga atttaaaggt
     8761 gaaatagtag ttgtagatga accggctaaa gtaaaacccg cactcgatta tctgaaacag
     8821 cagaaagtgg tgggactgga taccgaaacc aaaccatcgt tccagcgtgg caccatccac
     8881 aaggtatcgc tcgtccagat tgcatctgag gagaaatgct accttttcag gctgaataag
     8941 atcggtttcc caaacgaatt attacaattt ctgatggatg tgaacgtcaa aaaagtaggg
     9001 ctctcattgc gcgatgattt caacgggctc agcaaacgat ccatcattaa gcctcgtaat
     9061 gtggttgacc ttcagagtat tgccaaagat tacggcattc tcgaactggg acttcaaaaa
     9121 atgtatgcca tcctttttgg tcagaaaatt tcaaaatctc aacgtctgag caactgggaa
     9181 aatcccgaac ttaccgggca gcaaatgact tacgcagcca cagatgcctg ggcaagcctg
     9241 caaatttatc tgaaacttgt cgttaaagac aaactcagtc agaaagaaat tgacaaactg
     9301 atgtctgatg caaattctgc ccaacttaac agtgctaatt aaccgaaaaa attcaaatat
     9361 gattaccgta catcttaaac cgaaaaaaga agaaagttta ctccgtttcc atccctgggt
     9421 tttttcgggt gccattcagc acatcaacgg gaagcccacg gaaggtgagt tggtagatgc
     9481 gcttgataat cagggtaatt tcctggctac cggacactat cagattggca gcattgccgt
     9541 acgtgtgtta agtttcgaaa aggtagatat tgacaataat tactggcagc aaaaaatcag
     9601 tcaggcatat accgttcgca gggagttggg tctgattaat ccgccttaca acaatactta
     9661 ccggctcatt cacggcgaag gtgattcgct gccggggctt atagctgatg tttacgatga
     9721 tacagcagtg tttcaggcac attcagtagg tatgcatctt agccgtcagg ccattgctga
     9781 ggctattgta gcagtggttc cggctgttaa aaatgtgtat tacaaatcag aaaccaccct
     9841 tcctttcaat gctcctattg aaccggagga cggatacctg atagggaaag cgggaaatgc
     9901 gttcaaagcc atggaaaatg gacttgagtt taaggtcgat tggctgaaag gtcagaaaac
     9961 aggtttcttt gtggatcagc gggaaaaccg cgctctgctc gaaaaatatt caaaagggaa
    10021 aaaagtgctg aatatgtttt gctacaccgg tggattttcg gtgtattcgc tgcgaggtgg
    10081 agccgaactg gtgcactcgg tcgatagttc ggccaaagcc atcgaactca ccgacagcaa
    10141 tgtagccatc aatttccccg acgcaaaaaa tcatgtttcg tttgccgaag atgccttcaa
    10201 attcctcaac gataagcgcg aggaatatga tttgattgta cttgatccgc ccgcttttgc
    10261 caaacaccgc gaagctgtac gtaacgctct gaaaggttac aaaagactga acaggaaggc
    10321 tttggaacaa attaagccca acagcatcct cttcacattc tcctgttcgc aggtcatcaa
    10381 caaggaccag ttccggctgg cagtgttcag tgcggcagcc gaagccaaac gtaatgtgag
    10441 aattctgcac cagctgaccc aatcggcaga ccatcccatc aacatttatc atccggaggg
    10501 cgaatacctc aaaggactgg ttttgtttgt cgaataatta cggcacaata tttgctgtgt
    10561 caaaattgta tttggctgca atatttgttg ctgttttata tttttttaat tttttcacaa
    10621 caaattgtta ttttttttgg aattaaaaaa aggattacat acatttgcac ccaataaatt
    10681 atttattcac ctcctaaaaa ataatattta tacgcctatt gccaaaaatt actttaaact
    10741 aaaaatcagg aagtaatgaa aaacatttgt cagctaaccg acgaaatttt ggttaagttg
    10801 tatgaagatg gcaacaatca ggcattcgaa atattattat cgcgctacaa atcaaaagtt
    10861 tacacgtaca tttatctaat tgtacgcaac aaggaactta ccgaagacat atttcaggaa
    10921 acattcatca aagccattgc caccatccaa cagggccgct acgtagaaac cggacgattt
    10981 ctcggctggg taaaccgcat agcccataat ctgatcattg accattttcg ccgcgaaaaa
    11041 aatgaaaaca cattttctgc cgatgggctg gagtacgaca ttatcaacaa ctccaaactt
    11101 tcggaaaaaa gcgtggaaga taccatcagt aatgaacagg ttctcaccga tgtcatacat
    11161 cttattgatt atctacccca atctcaacag gaagttgtca gaatgaggtt tttcgaagat
    11221 ttgagtttca aagagattgc cgagcgcacc gaagtaagca tcaataccgc ccttggaaga
    11281 atgcggtatg cgctcttgaa tatgcggcgc attgctcacg aaaatgacat acaactggaa
    11341 ttgaaataat ttttgaattt catatactat aatgcaaaag ctttcgtttg attattataa
    11401 aaaaaaacga aaaaaagcct atgcgaaaag tttctactct ggttcattca actctaaaaa
    11461 ttaattcaca gaaagtttca acggtatcag caatgaaccc gagcgaaaca tcggttaata
    11521 aaatactgca gttcgctgct tcttacagag ttgagaaagt ttctgaaaat gaatttgtgg
    11581 aattattact taattaaaaa gaaagaggtt agctaactaa cctctttttt aactgttcaa
    11641 aatgccatca ccattgatgt gcaaaattac cccagtggat attactttgt aattttgaga
    11701 gacaagaccg gaataaccta tcggactaaa ttcatcaaga aataaatcaa ataatcattt
    11761 ataagagaga ct
//
LOCUS       DAOPZK010000061        11686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_6251_length_11686_cov_23.122603, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000061 DAOPZK010000000
VERSION     DAOPZK010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6251_length_11686_cov_23.122603"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKH68_05240"
     CDS             complement(<1..502)
                     /locus_tag="PKH68_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNX12374.1"
                     /translation="MSGKRDYYEILEVSKSASADEIKKAYRKKAIQYHPDKNPGDKVS
                     EEKFKEAAEAYEVLSDPQKRQRYDQFGHAGVGSSAASGGGFGGGMSMDDIFSHFGDIF
                     GGHFGGFGGFGGFGGSQRSSGQRVRRGSDLRVKVKLNLAEVAAGVEKKIKVKKYVSCS
                     HCNGSGA"
     gene            complement(562..1152)
                     /locus_tag="PKH68_05245"
     CDS             complement(562..1152)
                     /locus_tag="PKH68_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNX12375.1"
                     /translation="MKTRKHSSDNEKLTDQIPTEETKTKSTNGETALNSEETTENEAA
                     KIAEDLEQVNQKCEELNDKNLRLMAEFDNFRKRTLKEKSELIKNAGENIFVNILPLVD
                     DFERAMKAMETTEDIEAVKEGINLIYSKFMSFLNTNGVKVIPTDNEPFDVDKHEAVTL
                     YPAPTEDQKGKIIDCLTRGYTLNEKVIRFAKVVVGE"
     gene            complement(1352..3382)
                     /locus_tag="PKH68_05250"
     CDS             complement(1352..3382)
                     /locus_tag="PKH68_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNX12376.1"
                     /translation="MKSKILLLLSLFVCLNIFGQIQEPVKWTFEKKITGKNTADLIFH
                     ARIDDTWHMYGLNIPPDGPKPTAIVIETIQNAEKTGGIQAKSKLIESYDPNFDMKLNW
                     YAKDAVFVQKITFTDPEKIYVKGYVEFMCCDDKSCLPPTKEDFSFGKPQPNSSTAKQT
                     EIPAQQIEQNLPAVTSPVLLSSVTADSTQAQTTVILDSAKIAGLWTPVVSELQNYGGS
                     EGHSGARSFWWIFIAGLIGGFLALFTPCVWPIIPMTVSFFLKRSDNKARGRRDAALYG
                     LAIIFIYVLLGILITLIFGASALNSLSTNAVFNLLFFALLLVFAISFFGAFEITLPAS
                     WSTKMDAKADSTAGILSILFMAFTLVLVSFSCTGPIIGTLLVEVSGSGSILAPAIGML
                     GFAVALAIPFTLFAFFPSWLKSMPKSGGWLNKVKVVLAFLELALSLKFLSVADLAYGW
                     HLLDRETFLALWIVIFALLGFYLLGKLRFPHDSESKHTSVSGTMLAIISLAFAVYMIP
                     GLWGAPLKAVSAFAPPLYTQDFNLYDNEVHPQFKDYEAGMAFAASQNKPAIIDFTGFG
                     CVNCRKMEASVWTDPRVKRILDEDFVLISLYVDDKTPLAQSYQVTENGRTRTISTVGD
                     KWSYLQRHKFGANAQPFYVMLDNQGKPLNKSFAFDENPDHFIQWLERKNIDK"
     gene            complement(3532..10965)
                     /gene="sprA"
                     /locus_tag="PKH68_05255"
     CDS             complement(3532..10965)
                     /gene="sprA"
                     /locus_tag="PKH68_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNX12377.1"
                     /translation="MFFKKKIFPLLILFTALVGTAYGLAGSSRHYDLEAARDYQQPTS
                     AVDTATRYPVNNQRKETYDDLNKQYPMDLRNPDNVKSVVEYDVKTGNYVLTTRVGEME
                     ISTPYTLTPDEYQKYSLQNDMTKYWREKNSATVKSNEDKFSVTDMKFSLGPADKLFGP
                     GGVQLKTQGSAELIFGFKSNFIDNPALTQRARRSNIPNFDQKIQLNVNGKVGDKINFG
                     LNYNTEASFDFDQKLVKLSYKGKEDDIFQNIEAGNVSMQLNSSLITGSTALFGIKTDM
                     QFGKLKVSAIASQQESQSQTVSSKGGSQTIPFEVPIDQYDDNRHFFLSHFFRNNYESA
                     LSKLPFISSGVIINRVEVWITNKRGNFDQARNVLAFMDLAETDSIDNPHWQPVGGKKI
                     PYNKANTLYDEVTAIDGMRDVKQSNALLASLYGGYNINGGEDYEKIESARRLDPSEFT
                     FNQSLGYISLRSSLYPDEVLAVAYEYSYEGNVYQVGEFSTDGITAPNALVLKLLKSTT
                     QSPQLKTWDLMMKNVYSIGAMQMQKEDFELNVMYQNDSVGTMLQYLTEGEIANKRLLT
                     VMNLDRLDRKNEFNPDGNFDFVEGITAQSSSGRIIFPVLEPFGSHLRKKLNNAALASK
                     YVYQELYDSTLVVAQEYSEKNKFLLVGRYKGSANGSEISLNAMNVPRGSVVVTAGGAT
                     LTENVDYTVDYTMGSVTILNQSILESNTNIDVKLENQSMFSMQRKSLTGTHLEYVFSK
                     NFSLGGTIMHLSEMPLTKKVNTGSEPIKNTIWGLNTAWRTESQWLTNMLDKLPFVNAT
                     QPSNISFNAEFAQLLPGHSNVISNSGLAYLDDFESTKTNIDIHYPYNWYLASTPGLFP
                     ESALSDNVDYGKNRALMAWYFVDPVLNGESRQTPANLRGNAESQSDHRSRQVRETELF
                     PNKDLDPTRSSLLSVLNLSYYPNERGPYNLDVDGMNSDGTLSNPQDRWGGIMRRLETT
                     DFETANIEYIEFWMMDPFIADKNNEAGGDLYFNLGDISEDILKDGKKSFEHGLPINET
                     DANTTSTVWGRIPTTQSTVTAFDNAAGARINQDVGLNGLSTRQEFDFPAYRDYVEKVT
                     AKLSPDARTRMQNDPFSPLKDPAGDDYHFYRGTDYDQQKLSILQRYKYYNGTEGNSPA
                     TENTAETYSTSATLLPDMEDINSDNTLNEYEKYYQYKVALRPGAMKIGSNYITDVLDA
                     NVTLKNGKTETVRWYQFKIPIREYDDKVGSIRNFKSIRFIRLFLTNFRRETHLRFGTL
                     DLVRGEWKTYTKPLNALNAATASNSRLDVLAVNIEENGRKSPVNYVLPPGVTRETDPS
                     QPQLLQQNEQSMVLRVADLSAGEARAVYKNTTYDMRKYKRLQMFVHGEAMVDAAAQAQ
                     NLKDYELSCFIRFGSDMVNNYYEYEIPLKLTKPGVYLSDKLADREEVWQPSNMFDFPF
                     TVLTDAKLKRNKARQQGQTGVSNSIPFVVFDENNQNNKITIMGNPSISEVENIMIGIR
                     NVSGNTKSGEVWVNEMRMSEFDENPGWAGLANLSVGLSDLGSLNVSGRAETSGFGGLE
                     STITNRSLDDTYQMNLSAALDLGRFLPEQIKLQIPAYFTYTTETVSPKYNPLDNDILL
                     RDALDNLQDKFAKDSLKNLSQSVVTTKSFNVTNAKINVKSKKPKFYDPANVAFTLSSS
                     ETDEHTPEIFQNLNKQQRAAVNYSFSFNNEPWEPFKKSKGMKSPALKLIKDFNVYYLP
                     TTLSYNTSLNRVYSQILLRDLISGVSPLAQPDGMGNISSSKDFMWNRQFDLQYNITRS
                     LKVGLQTATNANIAEPRFTPEIGKEYYEQWRDTVWYNIKKMGTPYTYQQVFDASWKIP
                     IDKLPLLDWVTSNAQYNSTYSWNRSATMEGGSDIGNVASSMGSFSLDGTLNFENLYNK
                     VKYLKDVNTRSQAALRSQPYVSKTFRQTVNLKAGTPLTVNHALGSQRFSFTAKDKNGK
                     TVSVKYTVKNAGTIELNPTADIENVALTIVSQNPNYREPGQKIADFTTRLLMAVRRAS
                     VTYRQTSSLVMPGYKYQPGLLGIQTVDGMSAPGWGYVFGYHDSNTLKNAADNGWLYMG
                     KDIINPANEALTSDLDVRASVEPIAGFKIDLTAKRQTASNTVVHYMYEGMPSTFNGNF
                     NITQIALATAFDKIGTADQNYFSKTFDTFLANRQIVANRLNGKYTGTRYPTTGFFGES
                     PDYANKEYNPANGAFDLNSPDVLIPAFLSAYTGRDPNAVSTNPFLSILEMLPNWNVNF
                     NGLSSIGWIKEKVRSVSLTHAYTNRYTIGNYTSFSTWVPMDGDNSALGYIRDVQYNTP
                     IPSSPYDISTVSLNESFSPLIGVNVAMKNSMTTKAEYRKQRNLSLNLTSTQLIEAAAD
                     EFVVGVGYVVNDFDVMLKMKSSKRQTVKNDLKLNADFSYKDIKTLLRKVNENITQASS
                     GNKVVSIKFTADYVFSSKVNIQLFFDRQMTAPLVSSTFPVSSTNFGLSFKFLLAR"
     gene            complement(11040..11630)
                     /gene="ruvA"
                     /locus_tag="PKH68_05260"
     CDS             complement(11040..11630)
                     /gene="ruvA"
                     /locus_tag="PKH68_05260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNX12378.1"
                     /translation="MIDYIKGQITELTPANVVLETAGVGYFINISLTTYTALNAKTEA
                     QLFVYEAIREDAYQLFGFMNRDERQLFLLLISVSGIGANTARVIMSSYTASEIQQMIA
                     GGDVVSLNAIKGIGTKTAQRIILDLKDKILKVGTGTEVKGGQYPATSVVRDETVAALV
                     MLGFAATASQKVVEKIIQDDPSLSVEKVLKLALKML"
BASE COUNT         3003 a         2593 c         2623 g         3467 t
ORIGIN      
        1 ctgcacccga accattgcaa tgcgagcagg aaacatattt cttaacttta attttctttt
       61 caacaccggc agccacctcg gccagattaa gtttgacttt cacccgcaaa tctgaaccgc
      121 gtcgtacacg ctgtccgctg ctgcgctgtg agccgccaaa acccccaaat ccgccgaaac
      181 caccaaaatg tcctccaaaa atatcgccaa aatgagagaa aatatcatcc atgctcattc
      241 ccccaccgaa tccacctcca ctcgctgcac tgctaccgac acctgcatga ccaaactgat
      301 cataccgctg acgtttttgg ggatcgctca ggacttcata cgcttcggca gcctccttga
      361 atttttcctc agatacctta tcacccggat ttttatcggg atgatactgg atggctttct
      421 ttcggtatgc ttttttgatc tcatcagcac ttgctgattt cgaaacttct aaaatttcgt
      481 aataatctct ttttccggac atatatattt tttgttattc aatgaaattc agcagtttat
      541 cactacaata ttttattcag gttattcgcc tactaccacc ttggcaaagc gtattacctt
      601 ttcgttaagg gtatatcccc tggtgaggca gtcgattatt ttacctttct gatcttcggt
      661 gggtgcagga tagagagtta cagcttcgtg tttatccaca tcgaaaggct cattgtcggt
      721 aggaattacc ttcaccccat tggtgttgag gaaactcata aacttggaat atatcaggtt
      781 aataccttct ttcacggcct caatatcttc ggtagtttcc atggctttca ttgctctttc
      841 gaaatcatcg accaggggta aaatatttac aaatatattt tcacctgcat ttttgattaa
      901 ttccgatttt tctttcagcg tacgcttgcg aaaattgtca aattcggcca tcagtcgcag
      961 atttttatcg ttcaactctt cgcacttctg gttcacctgt tctaaatctt cggctatctt
     1021 tgcagcttca ttttctgtag tttcttcgga attcagagca gtttccccgt ttgtggattt
     1081 ggttttggtt tcctccgtcg gaatttgatc ggttagtttt tcgttgtcag aactatgttt
     1141 tcttgttttc atttcaacac tgattatatt ttttattatt tatttttcaa ttttcattca
     1201 tcgaattgtt tggcagtccg aaaccggaca aattacacca tttttgtcat tacaataaac
     1261 ctgccagaat gtccgaaact gatttttggc agaaatcagg acattttggc aggaaaataa
     1321 aaaatttaac tgacaaaact gatgacaaaa tttatttgtc aatatttttt cgttcgagcc
     1381 attgaataaa atgatcgggg ttctcgtcaa atgcaaagga tttattgagc ggctttccct
     1441 ggttgtcgag cattacatag aatggttgtg cattagcccc gaatttatga cgctgcaaat
     1501 agctccactt atcacccacc gtactaatgg ttcgggtacg gccgttttcg gttacctgat
     1561 acgattgtgc cagtggtgtt ttatcgtcca catacaatga aatgagtaca aaatcttcat
     1621 cgaggatccg ttttaccctt ggatctgtcc agacagatgc ttccatttta cgacagttga
     1681 cgcagccaaa accggtaaaa tcgatgattg caggtttgtt ttgtgatgct gcaaaagcca
     1741 ttccggcttc gtagtctttg aactgcggat gcacttcatt gtcatacagg ttgaaatcct
     1801 gggtgtagag cggtggtgca aaagcactta cagcttttag cggcgcgccc cacaatccgg
     1861 gtatcatgta cacggcaaat gccagtgaaa tgattgcaag cattgttccc gaaacggaag
     1921 tgtgtttcga ttcactgtca tgcgggaaac gcaatttgcc gagcaggtaa aatccaagca
     1981 aggcaaatat cacaatccat aatgctaaaa atgtctcgcg gtcgagtaaa tgccaaccgt
     2041 acgccaaatc agcaacagac agaaacttga gcgataaggc cagttcgagg aatgccagca
     2101 caactttcac cttattgagc catccgccgg atttgggcat ggatttgagc caggacggga
     2161 agaaagcgaa cagggtaaac ggaatggcca gcgcgacagc aaaaccgagc ataccgatag
     2221 ccggagccag tatagaccca ctgcccgaaa cttcgaccag caaggttccg atgatgggac
     2281 ctgtacagga aaacgacacc agtaccagtg taaatgccat aaacaggata ctcagaattc
     2341 cggcagtaga gtctgctttt gcgtccattt ttgtggacca ggaggcagga agtgtaattt
     2401 cgaacgctcc gaaaaacgag attgcaaaca ccaacagcag ggcgaaaaac agcaggttga
     2461 atacagcatt ggtggagagg ctgttgagtg cactggcacc aaaaataagc gtaatgagaa
     2521 tgccgagcag aacatatata aaaataattg ccagaccata aagagcagca tcccggcgac
     2581 cacgggcttt attgtccgac cgtttgagaa agaaactcac cgtcatgggg ataataggcc
     2641 acacgcaggg tgtaaacagt gccagaaaac cacctatcaa accggctata aaaatccacc
     2701 aaaaagagcg ggcacctgag tgaccttcag aacctccgta attttgcagt tcgctgacta
     2761 cgggtgtcca taaaccagca atttttgctg aatcaaggat aacagtagtc tgagcctgtg
     2821 tagaatctgc agttaccgac gataaaagaa ccggtgatgt aactgcaggc agattttgtt
     2881 ctatttgctg agcgggtatc tctgtttgtt tagcggtgga actgttgggc tgaggttttc
     2941 cgaaactgaa atcttctttg gtgggtggca ggcagctttt gtcatcgcag cacatgaatt
     3001 ccacatagcc tttcacgtag attttttcgg gatcagtgaa ggtgattttc tgtacaaaaa
     3061 cggcatcttt ggcataccag ttgagtttca tgtcaaaatt gggatcgtat gattctatca
     3121 attttgattt agcctgaatt ccgcccgttt tttcagcgtt ttgaatagtt tcaatgacaa
     3181 tagctgttgg ttttggccca tcgggaggga tattcagacc atacatatgc caggtatcat
     3241 cgatacgggc atgaaatata agatcggctg tatttttacc ggttattttc ttttcaaaag
     3301 tccattttac cggttcttgt atctgcccga agatattcag acatacaaaa agtgaaagca
     3361 gcaacagaat ttttgatttc ataaaggcaa tttttaaatt aagctacaaa attactaaca
     3421 aataagagat agtccgttct gattttgcat tctttttgga tatttttaaa aaacaacctt
     3481 tacacaaaca atgaagttgt agtaaaggtt gattgataac gaatgatggc attatctggc
     3541 cagcaagaat ttgaaactca gaccgaagtt ggtcgatgac accgggaatg tggacgaaac
     3601 aagcggtgca gtcatttgtc ggtcgaagaa gagctgaata ttgaccttgg aactgaacac
     3661 ataatcagca gtgaacttga tggacaccac tttattgcca ctggatgcct gtgtaatgtt
     3721 ttcatttact ttccgaagca gcgttttgat atctttatac gaaaaatcgg cattgagctt
     3781 caggtcgttt ttaactgtct gacgtttgct tgatttcatt ttcaacataa catcaaaatc
     3841 gtttaccaca taacccactc caacaacaaa ttcatcggct gcagcttcaa tgagctgggt
     3901 gctggtgagg ttgagggaca ggtttcgttg ttttcggtat tcggccttcg ttgtcatgct
     3961 gtttttcata gccacattaa ctcctatgag cggactgaaa ctttcattca gcgatacagt
     4021 tgaaatatcg taaggcgacg atggtatggg tgtgttgtac tgcacatcgc ggatataacc
     4081 cagcgcactg ttgtcgccgt ccattggcac ccaggtagag aatgaagtat aattgccgat
     4141 ggtatagcgg ttggtatacg catgtgtcag acttaccgaa cgtacttttt ccttaatcca
     4201 gccaatgctt gataatccgt tgaaattcac gttccagttt ggcaacattt ccagtattga
     4261 caggaacgga ttggttgaaa ccgcattggg atcccgcccg gtataagccg ataaaaatgc
     4321 aggtattaac acatcgggtg aattaagatc gaaagctcca ttggcaggat tgtattcctt
     4381 gttggcataa tcgggacttt cgccgaaaaa tccggttgtg ggataacggg ttccggtata
     4441 ttttccattc agacggttag cgactatttg tcggtttgcc aggaaagtat caaatgtttt
     4501 ggaaaaataa ttctgatcgg ctgttcctat tttatcaaat gcggttgcta atgctatctg
     4561 agtgatatta aaatttccat tgaatgttga aggcattccc tcatacatat aatgcaccac
     4621 ggtattggaa gccgtttgtc gtttggctgt caggtcaatt ttgaaaccgg caatcggctc
     4681 aacgctggcg cgaacatcca gatcggaagt cagtgcttca ttggcaggat tgataatatc
     4741 tttgcccata tacagccagc cattgtcagc ggcattttta agtgtattac tatcatgata
     4801 gccaaagaca tatccccagc cgggagcgga cataccatcc actgtctgta tgcccagcaa
     4861 accgggttga tatttatatc cgggcattac gaggctgctt gtctgacggt aggtaaccga
     4921 tgcacggcgc actgccataa gcaggcgggt ggtaaaatcg gcaatttttt gtccgggttc
     4981 acggtaattc gggttttggc taacgatggt taacgccaca ttttcaatat cggccgtggg
     5041 gttcaactca atagttcctg cattcttcac cgtatatttc acgctgacag ttttaccgtt
     5101 tttgtcttta gccgtaaatg aaaaccgctg acttcccagg gcatgattca ccgtgagtgg
     5161 agttccggct ttcaggttta cggtctgacg gaatgttttt gaaacatagg gttgtgacct
     5221 caatgctgcc tgcgaacggg tatttacgtc tttcaggtat ttaaccttat tataaagatt
     5281 ctcaaaatta agcgttccat caagggagaa tgatcccatt gatgatgcta cgttgcctat
     5341 atcggagccg ccttccatgg tagcactgcg gttccagctg taagtggaat tatattgtgc
     5401 attcgaagtc acccaatcca gcagtggaag tttgtcgatg gggattttcc acgaagcatc
     5461 gaatacctgc tggtaagtat agggtgtgcc cattttttta atattatacc acacggtgtc
     5521 tctccactgt tcatagtatt cctttcctat ttcgggtgta aaccggggtt cggcgatatt
     5581 cgcattcgtg gcagtttgga gtcccacttt cagactgcgg gtaatattat attgaaggtc
     5641 gaactgccgg ttccacataa aatctttgct ggacgaaata tttcccatcc cgtcgggctg
     5701 agcgagtggt gaaactcctg aaatcagatc acgaagcaga atttgagagt aaactctgtt
     5761 cagacttgtg ttgtagctaa gcgtagtggg tagataatac acattgaaat ccttgatcag
     5821 tttcagtgcc ggacttttca ttccctttga ttttttgaaa ggttcccagg gctcattatt
     5881 gaatgagaag ctgtagttaa cggctgcacg ctgttgcttg ttgaggttct ggaagatttc
     5941 aggggtatgt tcatccgttt cgctcgacga cagggtaaac gccacattag ccgggtcgta
     6001 gaatttcggt tttttacttt ttacattaat ttttgcattg gtcacattaa aacttttggt
     6061 agtgaccaca ctttgcgaca gatttttcag tgaatccttt gcaaatttat cctggagatt
     6121 atccagcgcg tccctaagca gaatatcatt atccagcgga ttgtattttg gcgatacggt
     6181 ttcggtggta taggtgaaat aagccggtat ctgaagttta atctgttcgg gcaggaagcg
     6241 tcccagatcg agtgccgccg acaggttcat ctgataggtg tcatccaggc tgcggttggt
     6301 aatggtgctt tccagacctc caaaacctga agtctcggct cttccggata catttaacga
     6361 ccccagatcg gataatccga ccgacaaatt ggcaagtcct gcccagccgg gattttcgtc
     6421 aaattccgac atacgcattt cattcaccca tacttcaccc gatttggtat taccactcac
     6481 gttgcggata ccaatcatga tattttccac ttctgagatg cttggattac ccatgatggt
     6541 gattttgttg ttctgattat tttcatcaaa caccacaaag gggatagaat tggaaacgcc
     6601 ggtttgtccc tgctgacggg ctttatttct tttcagtttg gcatcggtca ggactgtgaa
     6661 cggaaaatca aacatattcg agggttgcca cacttcttca cggtcggcca gtttatcact
     6721 caggtacact cccggtttgg tcaatttcag gggaatttcg tattcgtagt agttgttaac
     6781 catatccgat ccgaaacgga tgaagcacga caattcatag tccttcaaat tctgagcctg
     6841 tgctgcagca tcgaccatgg cttctccgtg aacaaacatc tgaagacgtt tgtacttacg
     6901 catgtcgtaa gtggtgtttt tatacaccgc acgggcttct cctgcactga gatcagccac
     6961 tctcagcacc atcgactgtt cgttttgttg caggagttgg ggctggctcg gatcggtttc
     7021 gcgggtgaca cccggaggca atacatagtt caccggactt tttcgtccat tctcttcaat
     7081 atttactgcc agcacatcca gtctggaatt cgatgcagta gcagcattca acgcattgag
     7141 cggtttagtg taggttttcc attctccgcg taccaggtca agtgttccaa aacgcaggtg
     7201 tgtttcgcgt cggaaattgg tgaggaacag tcgaataaag cgtatggatt taaaattgcg
     7261 tatgcttccc accttgtcat cgtattccct tatcggaatt ttgaactgat accagcggac
     7321 agtttccgtt ttgccatttt tgagggtaac gttagcatca agaacgtctg tgatgtagtt
     7381 tgatccgatt ttcatagctc cgggacgcag ggctaccttg tactggtaat atttttcgta
     7441 ctcgttgagt gtattgtctg agttgatatc ttccatatcg ggcagcaggg tggccgaagt
     7501 ggaatatgtt tctgctgtat tttcagtagc cggtgagtta ccttccgttc cgttgtaata
     7561 tttataccgt tgcagaatgc tgagtttctg ttggtcgtaa tcggtaccgc ggtagaaatg
     7621 ataatcatca ccagccgggt ctttgagtgg tgaaaacgga tcgttctgca tccgggtgcg
     7681 ggcatcgggc gagagtttgg cagttacttt ttccacataa tctctgtaag cagggaagtc
     7741 aaattcctga cgggtcgaaa gtccgttcaa tcccacatcc tgattgatac gggctcctgc
     7801 tgcattatcg aatgccgtga cggtggattg cgtggtggga attcttcccc aaacggttga
     7861 ggtggtattg gcatccgttt cattgatggg caatccgtgc tcgaatgatt tttttccatc
     7921 cttcagaata tcttcactga tatcgccaag attgaaatat aaatcaccgc ccgcttcatt
     7981 gtttttatcg gcgataaagg gatccatcat ccagaattcg atgtattcga tattggccgt
     8041 ttcgaagtcg gtggtttcca ggcggcgcat gatacctccc cacctgtcct gcggattcga
     8101 aagcgttccg tcggaattca ttccgtccac atccaggttg taaggacccc tttcattggg
     8161 gtaataggaa agattcaaaa ccgaaagcag cgaagagcgt gtcgggtcca ggtctttatt
     8221 gggaaataat tccgtttcgc gaacctgacg ggagcggtga tccgactgag actcagcatt
     8281 accccgcaga ttagcaggtg tctgacggct ttcgccattg agtaccggat ccacgaaata
     8341 ccacgccatc agtgcccggt ttttgccgta atccacattg tcgctcagag ccgattcggg
     8401 gaaaagtccc ggtgtacttg ccagatacca gttgtaaggg taatgaatat caatatttgt
     8461 ttttgttgat tcgaaatcat cgagatacgc caacccggaa ttgctgatca cgttggagtg
     8521 acccggcagc aactgtgcaa attctgcatt aaaagagata ttggacggtt gcgtggcatt
     8581 cacaaacggc agcttgtcga gcatgttggt gagccactgc gattcggtgc gccaggcggt
     8641 attcagtccc cagatagtat ttttaatagg ttcactaccg gtattcactt ttttggtgag
     8701 cggcatctcg gagaggtgca tgatggttcc acccaggctg aagtttttgc taaagacata
     8761 ttcgagatga gtacccgtca gcgatttgcg ttgcatgctg aacatggact gattttcgag
     8821 tttcacatcg atattggtgt tcgactccaa aatggactga ttgagaatag tcaccgatcc
     8881 catggtgtaa tccaccgtgt aatcgacatt ttcggtaagt gtagcaccac cggcagtgac
     8941 cactaccgaa ccgcggggaa cattcatggc attaaggcta atttcggagc cgttggctga
     9001 gcctttgtac cgccctacga gcaggaattt gtttttttcg ctgtattcct gagctacaac
     9061 gagggtcgag tcatacaact cctgataaac atattttgat gccagagcgg cattattcag
     9121 tttttttcgc aggtggctgc cgaaaggttc cagaaccgga aagataattc ttcccgaaga
     9181 actttgggct gtaatccctt ctacaaagtc aaaattacca tcaggattga attcattttt
     9241 tctgtcgagc cggtcgaggt tcataaccgt gagcagccgc ttattggcaa tttcaccctc
     9301 ggtgaggtat tgaagcatgg tgcccaccga gtcgttctga tacatcacgt tcagttcgaa
     9361 atcctccttc tgcatctgca tggctccaat ggagtataca tttttcatca tcagatccca
     9421 ggttttcaac tgcggcgatt gagtagtact tttcagcaat ttgagcacga gagcattggg
     9481 ggcagtaatt ccatctgtcg aaaattcacc gacctgatac acatttccct cataagaata
     9541 ctcgtaagca accgcaagca cctcatcggg atacaaactt gaacgcaatg aaatatatcc
     9601 aagtgactga ttgaatgtaa attcggaggg atcgagcctg cgggcacttt cgattttctc
     9661 gtaatcctct cctccgttaa tattataacc gccatacagg gaagcaagca gtgcattcga
     9721 ttgtttcaca tcgcgcattc cgtcaatcgc tgtcacttca tcgtaaagtg tatttgcctt
     9781 gttgtaaggt atttttttgc ctcccacagg ctgccagtga ggattgtcga tggaatctgt
     9841 ttcggccagg tccatgaatg ccagcacatt acgcgcctga tcaaaattgc cccgtttgtt
     9901 ggtaatccag acttccaccc ggttgataat caccccggaa gatataaacg gaagtttgct
     9961 gagggcgctt tcataattat tgcggaaaaa atgggacaga aaaaaatgcc gattgtcatc
    10021 atactgatct atgggcactt cgaaggggat agtctgcgaa ccgcccttgg agctgacggt
    10081 ttgcgattgg gattcctgct gtgaagcaat ggccgagact ttaagtttac cgaactgcat
    10141 atcggtttta ataccgaaaa gcgcggtact tccggtaatg agcgaactgt tgagctgcat
    10201 gctcacattc ccggcttcta tattttggaa tatatcatct tctttgcctt tgtaggaaag
    10261 tttcaccagt ttctggtcaa aatcgaacga tgcttctgta ttgtaattga gcccgaaatt
    10321 gattttatcg cccacttttc cattcacatt caactgaatt ttctggtcaa agttggggat
    10381 gttcgatcgg cgtgcacgtt gggtaagtgc cggattatca ataaaattgc ttttgaaacc
    10441 gaaaataagt tctgccgatc cctgagtttt gagctgtacg ccacccggac caaaaagttt
    10501 atcggcagga cccagactga atttcatatc ggtaaccgag aatttgtctt cgttgctctt
    10561 aacagtggca gagttttttt cccgccagta cttcgtcatg tcattctgca gggaatattt
    10621 ctgatattca tcgggggtca gtgtgtaggg agtggatatt tccatttcac caactcgcgt
    10681 tgtcagcaca taatttccgg ttttcacatc atactccacc accgatttca cattatcggg
    10741 atttctcaaa tccatcggat attgcttgtt cagatcgtcg tacgtttcct tacgctggtt
    10801 gttgaccgga taacgggtcg cagtatcgac agcagatgtt ggctgctgat agtcacgggc
    10861 agcttccaag tcataatgcc gtgatgaccc cgctaacccg taggcagtac ctaccagggc
    10921 ggtgaaaagt attaatagag gaaatatctt ttttttgaaa aacataaatt actgatttac
    10981 tacaacataa cgaaacagaa ttttccaaca aacagcttaa cagtttattt ccatatctgt
    11041 taaagcattt tcaaagcaag tttaagtact ttttccaccg acagcgacgg atcatcctgt
    11101 atgatttttt ccaccacttt ttgcgaagcc gttgcggcaa acccaagcat aacgagtgca
    11161 gctactgttt cgtcgcgcac cacgctcgta gcggggtact gaccaccttt cacctcagtg
    11221 ccggtgccca ctttgagtat tttatccttc aaatccagaa tgattcgctg ggcggttttt
    11281 gttccgatac ctttgatggc attgagcgaa accacatcgc ctccggctat catctgctga
    11341 atttcggatg cggtatagga tgacatgatt acacgggctg tattggcacc aattccggaa
    11401 actgaaatca acaacaaaaa cagttgccgt tcatctctgt tcataaaccc gaaaagctgg
    11461 taagcatctt cgcgaatagc ctcataaacg aacaactgag cttcggtttt agcattcaat
    11521 gccgtataag ttgtcagtga gatattgatg aaataaccca ccccggctgt ttccagcacc
    11581 acattggcgg gtgtcagttc cgttatttgg cctttaatat aatcaatcat gaattttaat
    11641 tttataaact tacaaagata gcttttctca ctgatttttc aaaaca
//
LOCUS       DAOPZK010000062        11557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_6366_length_11557_cov_22.456703, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000062 DAOPZK010000000
VERSION     DAOPZK010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6366_length_11557_cov_22.456703"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKH68_05265"
     CDS             <1..657
                     /locus_tag="PKH68_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNX12379.1"
                     /translation="NVSRYSPREEKYNTISHAVGIIFGLLAGIVLLTVAIPTGNGWLI
                     GSYTVFAVFMTMMFTTSTLYHAEKKETRKFILRKFDHAAIYCLIAGSYTPFTLVLLRN
                     EGYWGWLLFAVVWTAAIAGVIFSFTNLQNAGKLETICYLAMSWVVIFAFKPLIHVLTA
                     ANSMQIFYWLIGGGVFYTLGTILYRMKKINYTHAVWHLFVLGGCICHAYSIWLIARIA
                     "
     gene            840..2027
                     /locus_tag="PKH68_05270"
     CDS             840..2027
                     /locus_tag="PKH68_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNX12380.1"
                     /translation="MSLLSDVMAKYDEPQKVKALGLYPYFRPIESDQDTVVTIDGKPV
                     LMFGSNSYLGLTNHPRLKEGAIKAVEKYGTGCAGSRFLNGTLDIHIELEERLAKLVHK
                     QAALVYATGFTVNSGVLPCVAGREDYLLFDDRDHASIIEGKRLSYAKQIKYRHNDMDH
                     LESQLKKCEPDKIKLIVTDGVFSMEGDVAKLPHMVELAKKYNASLYVDEAHSLGVFGK
                     TGAGVCEHFGVSDDVDLIMGTFSKSLGTIGGFIAADENIINALKHTSRTLIFSASITP
                     ASTGCVLAALDVMEEETWRKDALWANTNRAKEGFLKAGFEIGPTETPIIPLYVRDNLK
                     TFNLTRMLMDDGVFVNPVVSPAVSSEDTLIRYSLMATHTFDQIDESIMKISKAARQLG
                     IIE"
     gene            2272..3888
                     /locus_tag="PKH68_05275"
     CDS             2272..3888
                     /locus_tag="PKH68_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX12381.1"
                     /translation="MITVSNLAIQFGKRVLFSDVNLKFTAGNCYGIIGANGAGKSTLI
                     RMLSGDLDPTRGNIQFGHGERLSVLKQDHNEFNEFTVIDTVMMGHDVLWKIKEEKDAI
                     YLKEDFTDADGLRASELEEKFGEMNGWNAESDAASLLSGLGVKEEFHYTLMKEMSGKE
                     KVRVLLARALFGKPDNLLLDEPTNDLDVDTVIWLENYLADYENTVLVVSHDRHFLDAV
                     STHTVDIDYGKVTMFAGNYTFWYESSQLALRQQQNQNKKADEKRKELEEFIRRFSANV
                     AKSKQATSRRKMLEKLNVEEIQPSTRKYPGIIFTPDREPGNMVLEVAGLGKVADDGTV
                     LFRDVNFNVEKNDKIAFVSRDPRAMTAFFEIINDHDKAHEGTYNWGVTITTAYLPLDN
                     SSFFNTDLNLVDWFGQFSDDTTEFFIRGYLGKMLFSGEEIYKKASVISGGEKMRCMIA
                     RMMLKNANVMILDSPANHLDLESIQAFNNTLINFKGNVLMASHDREFVSTVCNRIIEL
                     TPRGIIDKQMDFDEYLTSDDIKTIRDKMYR"
     gene            4033..6096
                     /locus_tag="PKH68_05280"
     CDS             4033..6096
                     /locus_tag="PKH68_05280"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HNX12382.1"
                     /translation="MKEDDKSKFLNREISWLHFNNRVLQESANPQVPLIERLRFLGIF
                     SNNLDEFFRVRVATLQRMAEMNMKSVSFREKPETILKKISKMTASTQTEMEGIYHGIV
                     EELKKERIFLVNEQELNETQTAFVKDYYDKQLEDAITPIMLSGDRTFPEMRDAKIYLF
                     VRLKRNTNHSEKKYALIEIPGSEYSRFLELPESNGNKYIMMIDDVIRLCMPQLFQSLP
                     YDVFETYTVKITRDSEMEIESELGEGIMEKVAKGIKSRRFGLPLRFVYDQLMPDEMRK
                     YLLKKIDFKKTGTIIPGGRYHNFKDFINFPVLRDNLVYAKTEPIENQLIRQSTSVLKS
                     IEKQDLFLHYPYYSFSQYVQLLREAAIDPDVKSIKITLYRMAHRSKVARALIFAARNG
                     KQVTAVVELRARFDEEHNILWSRQMEEAGVKIVFGVEGLKIHSKLTLISLKNGKKVAA
                     VSTGNFHEGNAAVYTDFTLLTAKSSITGEVERVFEFIGQPFQNQHFSHLLVSPQEMRK
                     KLTQLVNNEIANAKKGLPAYIHCKINHVTDYKFIDKLYDAAAAGVEIKMVIRGMCSMV
                     SSQPKLKGNLQVVGIVDKFLEHSRILIFCNNGLEKYYICSADWMTRNLDQRIEVSVPV
                     YDKEIQQELKTIVEYALKDNVKARIVDGKGENVLNENDEAPFRSQQELIMHYRNLEEN
                     RKPVE"
     gene            complement(6162..6578)
                     /locus_tag="PKH68_05285"
     CDS             complement(6162..6578)
                     /locus_tag="PKH68_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12383.1"
                     /translation="MIIAVDFDGTIVTHEYPKIGKEIPFAIDTLKRLQSEGMHQLILW
                     TVREGDLLAEAVDYCRERGLNFYAVNSNYPESERVDSQARKLSAELFIDDRNLGGVPD
                     WGVIYRMIMSGNPWTPLSADFMPDDLKTKKRGWFGK"
     gene            complement(6615..7088)
                     /locus_tag="PKH68_05290"
     CDS             complement(6615..7088)
                     /locus_tag="PKH68_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12384.1"
                     /translation="MERTIKNINLVYYLLYMTTVVVAVIIFFLTFTNTGEVYIEEKSQ
                     TGIIISSIYILYLLVSIPYALWSFHRKTKKWQQIKDQYVKLTEYEKGSKIRLWIIGIG
                     LIAGIVLVYFMHSKSMIFCAAIAAIALYFTKPTVNKMIAELRLDEEEDDMQSENN"
     gene            complement(7118..7738)
                     /gene="recR"
                     /locus_tag="PKH68_05295"
     CDS             complement(7118..7738)
                     /gene="recR"
                     /locus_tag="PKH68_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNX12385.1"
                     /translation="MNQQYSSTLLENAVNEFAKLPGIGRKTALRLVLHLLKQSEAEVD
                     AFGNAFISLRREIVYCKVCHNISDTEVCHICSNPGRDHHTICVVENIKDVMTIENTQQ
                     YRGLYHVLGGIISPMDGVGPGDLEIESLVKRVPDEEIKEVILALSTTMEGDTTNFYIF
                     RKLSPTNVKITTIARGIAIGDELEYADEVTLGRSIVNRVEFTNSFK"
     gene            8200..8781
                     /locus_tag="PKH68_05300"
     CDS             8200..8781
                     /locus_tag="PKH68_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12386.1"
                     /translation="MFSSQKTDVLFTIYNNNRTVYTFSNIALLTGESNAAKLSNKLNY
                     YVHSGKLLNPRKGIYAKNNYNPEELACLLYTPSYISLEYVLQKAGVIFQYDEKITAVS
                     YLSRTVEIDKNVYQYRKIKNEILIDMSGIIRSNNLNIATPERAFLDVMYLNASYYFDN
                     INSLNKNIIYELLPVYNSKILTERINNIFKKNG"
     gene            8774..9478
                     /locus_tag="PKH68_05305"
     CDS             8774..9478
                     /locus_tag="PKH68_05305"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNX12387.1"
                     /translation="MDSNKHRFFLLQILKDIYSDIELANLLGFKGGTSLMFFYDLPRF
                     SVDLDFNLLDKTKEKPVYDKIRKILLNYGKISDEAVKYYGIIVVLDYGAGERKLKIEI
                     SNRDFNDHYEIKNILGIDMKVLTLPDMFAHKLCALLDRNEMTNRDVFDCWFFLQRKTP
                     VNPEIVETRMQVSFNDYLQQCIDTLEKMSDKGLLNGLGELTTPEIKSFVRTKLRTETI
                     SLLRFFKEFPIIAKMK"
     gene            9517..11022
                     /locus_tag="PKH68_05310"
     CDS             9517..11022
                     /locus_tag="PKH68_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNX12388.1"
                     /translation="MQSYTVLLIIALYFAVLLLISHLVSRKGTGNDAFFRANRKSQWW
                     VVAIGMIGTSISGVTFVSVPGMVRDVSFNYMQTVLGFFFGYIVIARVLLPLYYKLNLT
                     TIYTYLDERFGRRAYKTGASFFLIGKTIGASARLYIVALILQQTVFDAWNVPFWVTVC
                     CTVLLIWLYTQRSGIKTVIWTDTIQTVIMIAALVLIIIEVTQRINLTNAELFTALKNS
                     PTTDIFVFGDWHSRQNFWKQFLSGVFIPIVMTGLDQDMMQKNLTIRTLKDAQKNMYWY
                     GIAFVPINFLFLTLGAMLLMLATQNGIALPAASDNILALFATQQLGTTVYVLFVIGII
                     AAAFSSADSALAALTTSFSVDILEVDKLGNDQKAVKIRRKIHIIIAIVFVFIILLFRE
                     INNKSVIDAIYTLVSYTYGPLLGLYAFGLFTKLKTNDKQVPYIAVLSPVICYILNFIS
                     TKYWNYSFGYELLMLNGLITFAGLWIFAPQTPTVSRPEASGYRKGKIGERE"
     gene            11121..>11557
                     /gene="yihA"
                     /locus_tag="PKH68_05315"
     CDS             11121..>11557
                     /gene="yihA"
                     /locus_tag="PKH68_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNX12389.1"
                     /translation="MEIKSAEFVISNTDYKKCPEGSKPEYAFIGRSNVGKSSLINMLC
                     NKKGLAMTSSKPGKTLLINHFIINDEWYVVDLPGYGYASTGKKMRVQLQNIIESYILN
                     REQLVNLFVLIDCRHEPQQIDLEFMEWLGENGIPFSIIFTKLD"
BASE COUNT         3478 a         2392 c         2418 g         3269 t
ORIGIN      
        1 aatgtaagcc ggtattcacc ccgggaagaa aaatacaaca ccatatcaca cgctgtggga
       61 ataatttttg gtctgttggc cggaatagta cttttaaccg tcgcaatccc gaccggcaat
      121 ggttggctga taggaagtta tacggttttt gccgttttta tgaccatgat gtttaccacc
      181 tccacacttt accatgctga aaaaaaggag acccgcaaat ttatcctgcg aaaatttgat
      241 catgcagcca tctactgtct catagcaggt tcatatactc ctttcacatt agtgttactt
      301 cgcaacgaag gttactgggg atggctgctc tttgcggtag tgtggacggc tgctattgcc
      361 ggtgtgatat tctcattcac caaccttcaa aacgccggaa aactcgaaac tatttgttac
      421 ttagccatga gttgggtggt gatatttgct ttcaaaccat taattcatgt attgactgct
      481 gccaattcca tgcagatatt ttactggctg ataggcggcg gagtatttta taccctggga
      541 actatacttt acaggatgaa aaagataaat tacacacacg ctgtctggca tttatttgta
      601 ttgggaggct gtatttgtca tgcttactcc atctggctga ttgcccgcat agcataaaac
      661 tggatattat ttcaattcct aaaacacatt ctttactact gataatcaga caaataagat
      721 attatcagga tttatcgccc acacccggtg attggtaaat gctaaaatta ttgaaacatt
      781 taaataaaaa aatagtatct ttgcaccctg aaaatattaa actataatta aatacattta
      841 tgagtttatt aagtgatgta atggcgaaat atgatgagcc acaaaaagtc aaagctttag
      901 gtctttatcc ttattttcgt cccatagaat cagatcagga cactgtagta accatcgatg
      961 gaaaacctgt attgatgttt ggatcaaaca gttacctggg actaaccaac cacccgcgcc
     1021 tcaaggaagg tgcaatcaaa gcagtagaaa aatatggcac cggatgtgcc ggatcacgct
     1081 tcctgaacgg aacgctggat atccacatcg aactggaaga acgattggcc aaactggttc
     1141 acaagcaggc tgcgctggtt tatgccaccg gttttacggt taattcggga gttttgccct
     1201 gtgtggccgg acgcgaagat tatcttctgt tcgatgaccg cgatcacgct tccatcatcg
     1261 agggtaaaag actttcgtat gccaagcaaa taaaatacag acacaacgac atggatcacc
     1321 ttgaatccca gttgaaaaaa tgtgaacccg ataaaatcaa actgattgtt accgatggcg
     1381 tattcagtat ggaaggtgat gttgccaaac tgccacacat ggtagaactg gctaagaaat
     1441 acaatgcttc actctatgtg gatgaagccc actcattggg tgtttttggc aaaacaggtg
     1501 caggcgtttg tgagcatttc ggcgtttccg atgatgtgga tctgatcatg ggtaccttca
     1561 gcaaatcgct cggaactatc ggagggttta ttgccgctga tgaaaacatc atcaacgccc
     1621 tgaaacacac ttcacgtacc cttattttca gtgcttctat caccccggct tctaccggat
     1681 gtgtgctggc tgcgctggat gtaatggaag aagaaacctg gcgcaaagat gctctctggg
     1741 ctaatacaaa ccgcgccaaa gaaggtttcc tgaaagccgg tttcgaaata ggaccgaccg
     1801 aaactccgat tatcccgctg tatgtgcgcg acaatctcaa aacatttaac ctcacccgaa
     1861 tgctgatgga cgatggtgta tttgtcaatc cggtagtttc acccgctgta agctccgaag
     1921 acacattaat ccgctactcg ctgatggcaa ctcatacttt cgatcagatt gacgaatcaa
     1981 tcatgaaaat ttcgaaagct gcccgtcagt tgggaattat tgaataagac aacctgcccc
     2041 ctatcgccca aaaagaggga ttgatggtcg gttgttcatg ttttttacat agatattttt
     2101 cacttatgat tattcacaat ttatcctaca ggatttcttt taggaattat tgtgaataat
     2161 cttatttttt atataaaccc ttgtaataac gctctttatt ccccctttcc cgatatcccg
     2221 aagcttcggg acgggacaca gtagggggtt agggggcaaa tcttttcttt tatgattact
     2281 gtatctaatt tagccattca gtttggcaag cgtgttttgt tttcggatgt caatctcaaa
     2341 ttcacagcag ggaactgcta cggtataatc ggtgccaatg gtgccggcaa atccacactc
     2401 atccgcatgt tgagcggcga cctcgaccct acacggggca acatacagtt tggtcacggc
     2461 gagcgtctgt cggtgttaaa gcaagaccac aacgaattta atgagtttac ggtgattgac
     2521 acggtaatga tgggacacga tgtgctctgg aaaattaagg aagaaaaaga cgccatttat
     2581 ctgaaagaag atttcaccga tgccgacggc ctgagggctt cggaactcga agaaaaattc
     2641 ggcgaaatga atggctggaa tgccgaaagt gatgctgcat cgctgctgag tggactgggt
     2701 gtgaaagagg aatttcatta caccctgatg aaagagatga gcggaaaaga aaaagttcgc
     2761 gtattgctgg cacgggctct gttcggcaaa cccgacaacc tgctccttga tgagcctacc
     2821 aacgacctgg atgtggatac cgtaatctgg ctcgaaaatt acctggccga ctatgaaaat
     2881 accgtgctgg tggtatcaca tgaccgccac tttctcgatg cagtaagtac gcatacagtg
     2941 gatattgact atggtaaagt gaccatgttt gccggtaatt atactttctg gtacgaatcg
     3001 agccagcttg ccctgcgtca acagcaaaat caaaacaaaa aagcggatga aaaacgaaaa
     3061 gaactggaag aatttatccg ccgtttcagc gccaacgtgg caaaatcgaa gcaggcaacc
     3121 agtcgccgta agatgctcga aaaactgaat gtggaagaaa ttcaaccttc tacgcgcaaa
     3181 tatccgggta ttattttcac tcccgatcgg gaaccgggaa atatggttct cgaagtggcc
     3241 ggactgggca aagtggctga tgatggcaca gttttgtttc gcgacgtaaa tttcaacgtg
     3301 gaaaaaaacg ataaaattgc tttcgtatca cgcgatccac gcgccatgac tgcatttttc
     3361 gaaatcatta acgaccacga caaagcgcat gaaggaacct ataactgggg tgttaccatc
     3421 actacggcct atctcccgct cgataattca agttttttca ataccgacct caatctggtg
     3481 gattggtttg gccaattttc cgacgatacc accgaattct ttatccgcgg atacctgggt
     3541 aaaatgcttt tctcaggcga agaaatttat aaaaaagcga gtgtaatatc gggaggtgaa
     3601 aaaatgcggt gtatgatagc ccgcatgatg ctaaaaaatg ccaatgtgat gatactcgac
     3661 tctcctgcca accacctgga ccttgaatcc attcaggctt tcaacaatac cctgatcaac
     3721 ttcaaaggca atgtgctcat ggcatcgcac gaccgggaat ttgtgagtac ggtctgcaac
     3781 cgcatcattg aacttacacc ccgtggaatt attgataaac aaatggattt tgatgaatat
     3841 ctgacatcgg atgatatcaa gacaatccgc gataaaatgt acagataaga caccgaatac
     3901 atccagatac ttaggaaacg attactatga aagcactgcg gataatctgc ggtgcttttt
     3961 tcttaacagc tttgtaatac atttatccag aaaaaaaact atatatttgt attttgtata
     4021 aatataagac ctatgaagga ggacgacaaa tctaaatttc tcaaccgcga gatcagctgg
     4081 ctgcatttca acaaccgggt tttacaggaa tctgccaatc cacaggtgcc tctgatagaa
     4141 cgtttgaggt ttctgggcat tttttccaat aatctggatg aattcttcag ggtacgcgtg
     4201 gctaccctgc agcgcatggc cgaaatgaat atgaaatcgg tttcattcag ggaaaaaccc
     4261 gaaaccatcc tgaagaaaat ttccaaaatg accgccagca cacaaactga gatggaggga
     4321 atttatcacg ggatagtgga ggaattgaaa aaggaacgaa tttttctggt caacgaacag
     4381 gagctgaacg aaactcagac ggcatttgta aaggattact acgacaaaca gctcgaagat
     4441 gccatcacgc ccatcatgtt atcgggcgac cggacttttc ccgaaatgcg cgatgcaaaa
     4501 atttatcttt ttgtcagact aaaaagaaat accaatcatt cagaaaaaaa atatgcactg
     4561 attgaaattc ccggctcgga gtattcacgc tttctggaac tgcccgaaag caacggaaat
     4621 aaatacatca tgatgattga tgatgtgatc cggttgtgta tgccgcagct tttccaaagt
     4681 ctgccttatg atgtttttga aacgtataca gtgaaaatta cccgcgattc ggagatggaa
     4741 atcgaatcgg aactgggcga aggcattatg gaaaaagtgg caaaaggaat taagagccgg
     4801 cggttcggat taccactccg gtttgtgtac gaccagctta tgcccgatga aatgagaaaa
     4861 tatcttctca aaaaaattga tttcaaaaaa acgggcacca tcattcccgg aggaagatac
     4921 cacaacttta aggactttat caattttccg gtattgcgcg ataatctggt ttatgccaaa
     4981 acagaaccca ttgaaaatca acttatccgc cagtcaacct ccgttctgaa atccatcgaa
     5041 aagcaggatt tgtttttgca ctatccttat tatagttttt cgcagtacgt gcaattgctt
     5101 cgcgaagctg ccatagatcc ggacgtaaaa agcataaaaa ttactcttta ccgcatggca
     5161 caccggtcga aagtagccag agcacttata tttgcggcac gtaacggcaa gcaggtaaca
     5221 gccgttgtag aattgcgcgc ccgtttcgac gaagagcaca atatcctgtg gtccagacaa
     5281 atggaggaag ccggtgtgaa aattgtattc ggagtggaag gactgaaaat tcacagtaaa
     5341 ctcaccctta tcagcctgaa aaacggcaaa aaggttgcag cagtcagcac cggaaatttt
     5401 cacgaaggca atgcagccgt ttataccgat ttcacactcc tgacagccaa aagcagcatt
     5461 accggtgagg tggagcgggt atttgaattc atcggacaac ctttccagaa ccagcatttc
     5521 agtcacctgc tggtttcgcc acaggaaatg cgtaagaaac ttacacagct ggtaaacaat
     5581 gaaatagcca atgcaaaaaa aggattgccg gcttatatcc actgcaaaat aaatcatgtt
     5641 accgattaca aatttatcga caaactatac gatgccgctg cagccggtgt agaaattaaa
     5701 atggtgatac gcggcatgtg ttccatggtg agttctcaac caaaactgaa aggaaacctg
     5761 caggtagtgg gcattgtgga taaatttctg gaacattcgc gcatactgat tttctgcaat
     5821 aacggattag agaaatacta catttgttcg gccgactgga tgacccgcaa ccttgaccag
     5881 cgcatagaag tgtcggtacc tgtgtacgat aaggaaattc aacaggaact gaaaaccatt
     5941 gtggaatatg ccctgaagga caatgtgaag gcccgtatcg tcgatgggaa aggtgaaaat
     6001 gtcctgaatg aaaacgatga ggcgccgttt cgctcacaac aggagttgat aatgcattac
     6061 cggaatttgg aagaaaacag aaaaccggta gagtaacacg ttgaattatt cggtttaatt
     6121 acacaaaaaa ccccggtcaa atctgccggg gttagttatt tttatttccc aaaccatcct
     6181 cttttcttcg ttttcagatc atcgggcata aaatcggccg acaacggtgt ccatggattt
     6241 ccggacataa tcatgcggta aatcacgccc cagtcgggca ctccgcccag attgcgatcg
     6301 tcaatgaata attctgccga aagttttcgt gcctgactat ccacccgttc cgattcggga
     6361 taattgctgt tgacagcata aaaattcagt ccgcgctccc ggcaataatc caccgcttcg
     6421 gccagcaaat ctccttcacg cacggtccag agtatcagtt gatgcattcc ttcactttgc
     6481 aaacgcttga gtgtatcaat ggcaaatgga atttcttttc ctattttggg gtattcatga
     6541 gtgacgatgg ttccgtcaaa atccacagca ataatcatag atcaattaca cgtttaggtt
     6601 taataaaaaa taaatcagtt gttctcagac tgcatgtcat cctcctcttc gtcgaggcgc
     6661 agttcggcta tcattttatt caccgtaggt ttggtaaaat aaagtgcaat agctgcaatg
     6721 gcagcacaga aaatcatgga tttggaatgc ataaaataaa caagtacaat gcctgctatc
     6781 agaccgatac cgataatcca gagacgaatt tttgatcctt tttcatattc ggttaatttg
     6841 acgtactggt cttttatctg ctgccatttt tttgttttac ggtgaaaact ccacaaggcg
     6901 taaggtattg acacgagcaa atacagaata tatatcgatg aaataattat tcctgtttga
     6961 cttttttctt cgatatacac ttcgccggta ttggtgaatg taagaaaaaa tatgatgaca
     7021 gcaaccacta cggtagtcat ataaagtaaa taatacacca gattgatgtt tttgatggta
     7081 cgttccatac tattgattat taatttatta cgtttattta tttgaatgaa ttggtaaatt
     7141 ccaccctgtt gacaatggag cgccccagtg taacctcatc ggcgtattcg agttcgtcac
     7201 caatggcaat tccgcgggca atggtcgtta tttttacatt tgtcggcgat aactttctga
     7261 aaatataaaa gttggtggta tcaccttcca tcgttgtgct caatgccaga ataacttctt
     7321 taatttcctc atccggtaca cgtttaacaa gactttctat ttccagatca ccgggtccaa
     7381 ctccatccat gggcgaaata atgccgccca gcacatgata caaaccacga tattgctgtg
     7441 tgttttcgat ggtcatcacg tccttgatat tctccaccac acaaatggtg tgatgatcac
     7501 gccccggatt gctgcaaata tgacacactt cggtgtcgga tatattgtga caaaccttgc
     7561 aatacacaat ttcccggcgc aaactgataa atgcattacc aaaagcatcc acctcggctt
     7621 ccgactgttt gagcagatgc aaaacgagcc tcagagccgt tttgcggcca atgccgggca
     7681 gtttggcaaa ctcgttgact gcattttcga gcagcgtgga tgaatattgt tggttcattt
     7741 attagctgag aaattatgtg caaatttagc aaaaaaatta catcttcact aattttgctc
     7801 aaaggcagat gggtgttttt tttaatcatt caaattcact aacaataaaa atatgcaaac
     7861 ctgtttatat cttgattgat atatcatttt tttacattgt tgtctggtaa aatatttaga
     7921 tccatcgctt cgctctggat gacaggaaat tatggtgttt taggtaagga ggggttggct
     7981 ccgtgaggac ggagccaacc ccctccttaa agataaaata ctgactgtca tttcgaacgt
     8041 cccgacagtt cgggatgaga attgaaaatc ggcgtagcca aatctaaaat atatcaagaa
     8101 tttagatttt taccggacaa tagtcatttt ttattggttt aatttgtttt tttgaaatac
     8161 ttcttttatt tttgtgaact aattatatga atttgaagta tgtttagttc acaaaaaaca
     8221 gatgttttgt ttactatata caacaacaat agaactgtat ataccttcag caacattgca
     8281 cttctgaccg gagaaagcaa tgcggctaag ctatccaaca aactgaacta ttacgttcac
     8341 agcggcaaat tgcttaatcc cagaaaagga atttatgcaa aaaacaacta caatcctgaa
     8401 gaactggctt gtttactata tacgccctca tacatttcac tggagtacgt gttgcaaaaa
     8461 gccggcgtca tatttcagta tgacgaaaaa ataacggctg taagttattt gagtcgcaca
     8521 gtggaaattg ataaaaacgt ttatcagtac agaaaaataa aaaatgaaat tttaattgat
     8581 atgagcggca ttataaggag taataattta aatattgcga ctcccgaaag ggcttttctg
     8641 gatgttatgt atctcaatgc cagttattat ttcgacaata ttaattcgtt gaataagaat
     8701 ataatatatg aacttttacc tgtttacaat tcaaaaatat taacggaaag aattaataat
     8761 attttcaaga aaaatggata gcaacaagca tcgttttttt ctgcttcaaa tcctgaaaga
     8821 tatttattca gatattgaat tagcaaattt attgggattt aagggcggta cttcgctgat
     8881 gtttttttat gatttacccc gtttttcggt tgatctggat tttaatttac tggataaaac
     8941 caaagaaaaa ccggtgtatg acaaaattcg gaaaatactg cttaattacg gaaaaatttc
     9001 agatgaagct gttaagtatt acggaataat tgtggtgctt gactatggag caggcgaacg
     9061 aaaattgaaa attgaaattt caaacaggga ttttaacgat cattacgaaa tcaaaaacat
     9121 attgggcata gatatgaaag ttcttaccct acccgatatg tttgctcata aactttgtgc
     9181 attgctggac agaaatgaaa tgaccaacag agatgtattt gactgctggt ttttcttaca
     9241 gcgaaaaacg cctgtaaatc cagaaattgt tgaaacaaga atgcaggtat cttttaatga
     9301 ctatttacag caatgtatag ataccctgga aaaaatgagt gacaaaggtt tgctgaacgg
     9361 attgggtgaa ttaacgacac ctgaaataaa gagttttgtc cggacaaaac tacgaacaga
     9421 aaccatctct ctattaagat ttttcaaaga atttcccatt attgctaaaa tgaaataaat
     9481 tcggaaattc ctactttcat tttcatccaa taaaacatgc aaagctacac tgtccttctt
     9541 atcatagccc tttattttgc cgttttgctg ctcatctcgc acctggtgag tcgtaaaggt
     9601 accggcaacg acgcattttt cagggccaac cggaaatcgc agtggtgggt ggtggctatc
     9661 ggtatgattg gtacttccat ttcgggagtt acgtttgtgt cggtgcccgg catggtgcgc
     9721 gatgtgagtt tcaactacat gcagacggtg ttgggatttt tctttggcta catcgtcatt
     9781 gcccgcgttt tgctgccact ttattacaaa ctcaatctca ccaccattta cacctacctg
     9841 gatgagcgat ttggccggcg ggcttacaaa accggcgcat cgtttttcct gatcgggaaa
     9901 actatcggcg cttcggcacg actgtatatt gtagcactca ttttgcagca aacggtattc
     9961 gatgcctgga atgtaccttt ctgggtcacg gtttgctgca ctgtattgct gatttggctg
    10021 tacactcaac gcagcgggat aaaaacagta atctggacag ataccattca aacggtcatc
    10081 atgattgccg ctttggtgct gataatcatt gaagtaactc aacgtatcaa tctgacaaat
    10141 gcagaattat tcaccgccct gaaaaacagc ccgacaaccg atatttttgt gttcggagac
    10201 tggcacagca ggcagaattt ctggaaacag tttctgagcg gtgttttcat tcccatcgtg
    10261 atgaccgggc ttgatcagga catgatgcag aaaaacctca ccatccgtac actgaaagat
    10321 gcacaaaaaa acatgtactg gtacggcata gcctttgttc cgatcaattt tctgtttctc
    10381 acgctgggtg ccatgctgct gatgcttgct acgcaaaacg gaattgctct gccggcggca
    10441 tccgacaata ttctcgctct ttttgccaca caacagctgg gtacaacggt ttacgtgctt
    10501 tttgtcatcg gcattattgc tgcagcattt tcaagtgccg actcggcgct ggctgcactt
    10561 acgacctctt tttcggtgga tatactggaa gtggataagc tcggaaatga ccagaaagct
    10621 gtgaaaatcc gccggaaaat ccatataatc atcgcaatcg ttttcgtctt tatcatttta
    10681 cttttcaggg aaataaacaa caagagcgtg attgatgcaa tttacaccct ggtgtcgtac
    10741 acatacggtc cattgctggg tttgtatgct ttcgggctgt ttacaaaact gaaaactaac
    10801 gacaaacagg tgccttacat tgccgtgctt tcacccgtaa tttgctatat tttaaatttt
    10861 attagcacaa aatactggaa ttattccttt gggtatgagt tgctcatgtt aaacggatta
    10921 attacatttg caggattgtg gatatttgcc ccccaaaccc ctactgtatc ccgtcccgaa
    10981 gcttcgggat atcggaaagg gaaaattggg gagcgagaat aagatttata attttatttg
    11041 tttttcttat aattcatttc accaatataa tttcaatttt tacactaaaa tccacccctt
    11101 gggggttagg aggcctatca atggaaataa aatcagcaga atttgtcata agtaatacgg
    11161 attacaagaa atgccccgag ggctccaaac ctgaatacgc ctttatcggg cgttctaatg
    11221 tggggaaatc ttctttgatc aatatgcttt gcaataaaaa agggttggcg atgacttcat
    11281 cgaaacccgg caaaacattg ttaatcaatc attttatcat caacgatgaa tggtatgttg
    11341 tcgatttgcc cggatatggt tatgccagca cgggaaaaaa aatgcgtgta caacttcaga
    11401 atatcatcga aagttacata cttaaccgcg agcaacttgt aaatcttttc gtattgatag
    11461 attgtcgtca cgaaccacaa cagattgatc ttgaatttat ggaatggctg ggtgaaaacg
    11521 gcattccttt ttccatcatt tttactaaat tagacaa
//
LOCUS       DAOPZK010000063        11301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_6565_length_11301_cov_28.488262, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000063 DAOPZK010000000
VERSION     DAOPZK010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6565_length_11301_cov_28.488262"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKH68_05320"
     CDS             complement(<1..248)
                     /locus_tag="PKH68_05320"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HNX12390.1"
                     /translation="MKTKNIYFALTGFAIFAVISFLFYQSFYLYHLAYREQTQLFLMS
                     GEYINTYFHHSAWMACLAGDFLTQFYCFNYTGALIITLS"
     gene            711..3971
                     /locus_tag="PKH68_05325"
     CDS             711..3971
                     /locus_tag="PKH68_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX12391.1"
                     /translation="MIRNLKIRKQQQFHFFNKMKIVVICVFLLLGGIATVSAVPISGD
                     ASNAMSQQNQKVTGRVVDKNGEPVIGANVIQQGTSNGTLTNGEGNFTLNLPGDATLVV
                     SSIGYTSQQIAVGSRSLINVTLEEDTKLLDEVIVVGYGTMKKKLVTGATVQVSGDNLQ
                     KLSTTSAFTALQSQTPGVNILQNSGQPGEGFKVSIRGVGTAGNSAPLYVIDGVAGGNL
                     QNLNNADIESIDILKDAASAAIYGARAANGVILVTTKQGKAGKLQVSYDGYYGVQNIY
                     KMPSLLNAKQYMAIQDEVNFNEGLALNDWKTILGKNYDNAVYNGTYGTNWLDEARNVN
                     APTQNHAVNIVGGTDISKFSMGLSYTAQEGIIGKPVASDYNRTTARMNSDHVLWKNKS
                     FDVIKVGETLTFAYSTKKGIGIGNQYSNDISSMLRAMPIMPLYGEDGQYFRNIGETLT
                     SYESLMSNPIAGMVYNRGQNLSKNYNLDASANIQIQPIKNLIFKSQLGYKMSASSYRQ
                     YQPDYTLSISDQRQVALAQQSASSGWQYSLENTLNYKFGLNLNHFDALVGQSMEKWGM
                     GESVSATNGHLLFSDFEHAYIDNSQGIESGVTRISGSPWGEGALASFFGRVNYDYNET
                     YLATIIMRADGSSNFARGKRWGYFPSASLGWVISNENFMQSTKGWLDYLKLRGSYGEN
                     GNQSIAAFQYLAGVSFDAKAAYSFGNAKDSQTTGGYAYIQPNPDVTWETSIQKNIGLD
                     ARLLNSRLSVAIDVYDKFTKDWLLQAPGLGTIGTGVPFINGGDIDNKGIEVALGWNDR
                     IGKDFTYGINVNGAYNHNEVTRIANTEGVIHGTVNVLSQGTGEMYRVQVGYPIGYFYG
                     YHTSGIFQNQAEIDAWKAAGNGVLQSKVQPGDVKFSDISGPDGKPDGSIDENDKGMIG
                     DPNPDFTAGGSINLGYKGFDFSVTLYGAFGQQIAKSYRKFGDGRHENYTTEVYERWHG
                     EGTSNKWPRLTAGTNANYMNISDLYIEDADFVKIQNLTIGYDFKKLFKSLPMSQARLY
                     FSAQNLFTFTGYSGMDPEVGYSPQDTDGTTQNWAKGIDLGFYPQPRTYLVGVNLKF"
     gene            3984..5708
                     /locus_tag="PKH68_05330"
     CDS             3984..5708
                     /locus_tag="PKH68_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007410771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX12392.1"
                     /translation="MKKIIYLAIITATVLFTGCESFLDTKDLTHKNTSNYPQTVTDAQ
                     QVIAGIYNNLNITHASPASSFLFISELASDDRFGGGGKQDFSFQSKDLLMQAGSNECE
                     QFWKDRYAGIFRANTAIETLANCSGYESDNQKNQMIGEAYFMRALYYYELASEFENIP
                     LVITSASGNPAQATPDQTWGQICADLLKAIELMPDNKANTFVAAGHADNWAAEALLGR
                     AFLFYTGFYEKAAVTLPDGTSLDKAAVVAKIDDCVNNSGYSLVPDFRNLWAYSNRLTK
                     EDYTYTKGQGLKWVEDDNGINPESMFAIKFSKFASWGTTIGYSNGNALYFGVRGGQDL
                     AKTFPFGQGWGCGPVNPALWQEWAAAEPTDIRRKATICEIPAELPNYTKGAASWNDFI
                     QETDYYAKKTAPISSFKADGVKCWETFEQDMYGYTSVNFQLSNIHDLVLIRFADVLLM
                     QSELKGDATGMNKVRARVGLPAVAYSLAALQKERRWEFACEGIRWNDMRRWHIAEAAL
                     GTQIGQNIYLQGAANKNTNQGAGYVARYKATRGFFPIPENQIALSNGTLKQNAGWGTA
                     DTNYTGWK"
     gene            5743..6585
                     /locus_tag="PKH68_05335"
     CDS             5743..6585
                     /locus_tag="PKH68_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12393.1"
                     /translation="MNKIFYIISAFILVALFSACEPIVNPVDMGTIITSADELQATVT
                     PVMDGTKKTNKVIVSCTSPVSCQWTDGVKTYTSNNTEMILFVPGDIEITLKAMAADGT
                     VLTKKFTTKVDEIKFPVEPQYGYFCGSGVKTWVWAVDNTYTGPQRIWGNGNSISSGVP
                     NWWGKSAADAEPDIDLTAKMTFTLNGLKFSRTERGVTTTGNFSFDMGSHKASTSIGSV
                     TFIGTTIPHGIAQNNGQKVVYTFDIKKLTNDEMILMYPTDPFTPGNDSEGWFWLFKRE
                     GFTY"
     gene            6743..7903
                     /locus_tag="PKH68_05340"
     CDS             6743..7903
                     /locus_tag="PKH68_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNX12394.1"
                     /translation="MNYLQKKYPDLNVTAEIAIVEPHHKVKEMQAIMHLDKPEGSFSQ
                     QIQSFRTAIVKVLSERELQHASVFSARWFLTDPANQQVQISAEMADVLDCPVCYIKQP
                     LLDGSKIALWMQLHTDMKVGNDGLSFHEHNNYRHYYTTDNSINNGNESVNSYEQTKQL
                     LEVYEKQLSDRECTIERDCLRTWFFVRDVDVNYQGVVEARKDNFRLNGLTENTHYIAS
                     TGIEGSTGDPDTKVVMDTYAVKGLDEGQVKFLYAKENLSPTYDYGVTFERGVSVDFGD
                     RRKVYLSGTASINHEGTILHPGDVEKQVYRMWENVEALLQEAECSFDDLMQLIVYLRD
                     IADYQSVKRMFDEKFADTPKLIVLAPICRPGWLVEMECIAAKPISNNNFRDF"
     gene            8070..9851
                     /locus_tag="PKH68_05345"
     CDS             8070..9851
                     /locus_tag="PKH68_05345"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HNX12395.1"
                     /translation="MNKISRIFSLRAFFSVLFGFVVFLFFLRYYQFHLHYEEQLQLFL
                     FTSDYWSQLISKPGGLADYIGTFFTQFYYVERLGALIIALLLVLLQSQVHSLTKKISK
                     NESFFPLTFIPSILIFALLCNENFLLSGLIALMLTLAAVRIYTLIKNPVVRIIGAIIV
                     APVLFWFAGGVFWICSLLCLILEWGYFKQLSKLQWTILIFGLLVTALSPVVFKHLFVL
                     QYPLSRLWWGLCYSRYPVVSPYPLLVAWLSVPLLVVAFRFLPVIKKQSVNIAVLLSLS
                     LIVAIFAAYFIRKSADFKKEEIMAYDYYARTQNWDKIIALANHKDPDSPLSVACLNLA
                     LSKTGKLGDSMFRYFQNGPEGLLPTFQRDFTSPFISGEIYYHLGFLNTSMRYNFEAME
                     AIPDYKKSARAVKRMAEVNLLNGEYKVAAKYLSYLQSTLFYRSWADETRKCIDNPKLI
                     EQNKEWSALKKYRLTEDFLFSEQDKDQMLGILFSHATTNKMAYEYLMAYTLLTKDIEH
                     FVKYFPLGKSLDYQEIPSHFQEALILFWSTQSADFDQTPWPITDPVKQYLVSYAKAYN
                     ANGRENADPRSMADFSQTYWYYYHFRK"
     gene            9869..>11301
                     /locus_tag="PKH68_05350"
     CDS             9869..>11301
                     /locus_tag="PKH68_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12396.1"
                     /translation="MNKYSSYILIFLLTLLVGCNESVKSPKLIDTFPAIYPDYTFVTV
                     PASIAPLNFTIKSVKYDVIDVVVTGKVNGTLHVQSDEIIQFPEKKWHNLLESNKGSDI
                     QVLVSLKSDGVWKQYKTFPVHISPNPIDYGLVYRMIAPGYEVYSKMGIYQRNLSDFDQ
                     KPIVENTLIPGSCVNCHAFSKNNPSFMSLHVRGTHGATMLQVNGKMQMLNTKTDSTIS
                     SCVYPYWHPSGKYIAYSTNKTKQAFHVVRNERVEVFDEASDVVVYDVNSNQLLTCDAL
                     KTSAFETFPVFSADGRALYFCSSKPYDIQSDYKKIKYSLCKIDFNPDNGTFGTKVDTL
                     VSSSKLNKSVSFPRPSFDGKYIMFTLSDYGNFSIWHREADLYLLTLSTGAFRPISEVN
                     SADTESFHDWTTNSRWFVFSSRRGDGLYTRLYLASMDENGKIGKPFLLPQKDPRFYDV
                     SLFSYNVPEFITAPVKVDVSEFERKAV"
BASE COUNT         3463 a         2034 c         2326 g         3478 t
ORIGIN      
        1 gacaatgtaa ttattaatgc tccggtatag ttaaaacagt aaaattgagt caggaaatcg
       61 ccggctaaac atgccatcca ggccgaatga tgaaaataag tgttgatata ctcaccagac
      121 attaaaaata attgtgtctg ctcacgatag gctaaatggt ataaatagaa agactgatag
      181 aataggaagg atataacagc aaatattgca aaaccagtaa gtgcaaaata aatatttttt
      241 gtcttcattc aagttattta cattaatatt atatagccga ttatgcccgg actaatttga
      301 aattattttt ttattgatgt ttttgatgcg caaatataca gaaaatgttt aaaatatgga
      361 aagtgtcgtg aataaactca aattggtcat gagataatac aattcaacat aaaacatgta
      421 ggttgattct gaaaatttaa aattacgaaa catatttttc tttttttata aataaaattt
      481 ggatattatt ttgctaaaac gtacatttgt cagctaaaat gttaaacatg taaagattgt
      541 ataatttatt gataaaattg tatctgtttt ttttgcagga aactcataga tttgcaacgc
      601 tcaataaata gaatttacgg agagaactat ttgattttac acacgattga atattaatga
      661 attaacatat aaaattaatt aaccttttta ttaatccaaa aaaactaagt atgattagaa
      721 atttaaaaat tagaaagcaa cagcagtttc attttttcaa taaaatgaaa attgttgtta
      781 tttgcgtttt tcttttgctt ggtggtattg cgactgtatc tgccgttcct ataagcggtg
      841 atgcaagcaa tgctatgtct cagcaaaacc aaaaagtaac cggacgagta gtcgataaaa
      901 atggcgaacc tgttataggt gccaatgtta tccaacaagg tacctcaaac ggcacattga
      961 ctaatggtga aggtaacttt actttaaatt taccgggaga tgctactctg gttgtatcaa
     1021 gtataggata cacctcccag caaattgcag ttggttcaag aagtcttatc aatgtaactt
     1081 tagaagaaga tacaaaactg ctggatgaag ttattgttgt tggttatgga actatgaaga
     1141 aaaaactggt tacaggagct actgttcagg taagtggaga caatcttcaa aaactcagta
     1201 ctaccagtgc atttactgcc ttacaaagcc agacaccagg cgttaatatc cttcagaact
     1261 caggacaacc gggtgaagga tttaaagtta gcatacgtgg tgttggtact gctggtaatt
     1321 cagctcctct ttacgttatt gacggagtag ccggtggtaa tcttcagaat cttaacaacg
     1381 ctgatatcga atcaatcgat attttgaaag atgccgcttc tgctgcaatt tacggtgcac
     1441 gtgctgccaa cggtgttatc cttgttacta ccaaacaagg gaaagccgga aaacttcagg
     1501 taagttacga tggctattac ggtgtacaga atatatataa aatgccgtca ttactgaatg
     1561 ccaaacaata tatggctatt caggacgaag ttaatttcaa cgaaggtctg gcgctaaatg
     1621 attggaaaac aatattggga aaaaattacg acaatgctgt ctataacgga acttatggta
     1681 ctaactggct ggatgaagcc cgcaacgtta acgctcctac acaaaatcat gcagttaata
     1741 ttgttggagg taccgatatt tctaaatttt caatgggact ctcttatact gctcaagaag
     1801 gtattattgg taaacctgtt gcctcagact acaacagaac cactgcccgt atgaattccg
     1861 atcatgttct ttggaaaaac aaatcctttg atgtgattaa agtcggcgaa acattaactt
     1921 ttgcttattc aaccaaaaaa gggataggta ttggtaatca atactcgaac gatatttcca
     1981 gcatgttaag agctatgcct atcatgcctt tatacggtga agatggccaa tattttagaa
     2041 atattggtga gactttgact tcatatgaaa gtttgatgtc aaatcctatt gctggcatgg
     2101 tttataaccg cggacagaat ttgtcaaaaa actacaatct ggatgcatca gcgaatatac
     2161 aaatacagcc tattaagaat ttgattttca aatcacaatt gggttataaa atgagtgcaa
     2221 gctcatatcg tcagtatcag cctgattata ctctttctat ttctgatcag agacaggttg
     2281 ccttagctca acaaagcgca agttctggat ggcaatattc attagaaaac acattgaact
     2341 ataaatttgg tttaaatctg aaccattttg atgctttagt aggacagtca atggaaaaat
     2401 ggggtatggg tgaaagcgta agtgcaacta acggtcatct tttattctct gattttgaac
     2461 atgcttatat tgataactca cagggtattg aatcaggagt aacaagaatc agtggttccc
     2521 catggggcga aggcgctttg gcatctttct tcggtcgtgt taactacgat tacaatgaaa
     2581 cttatcttgc tactattatc atgcgtgcag acggttcctc caattttgca agaggaaaac
     2641 gctggggtta ttttccatca gcttctttag gctgggtaat ctccaacgag aattttatgc
     2701 aatcaacaaa aggttggctt gattatttga aacttcgtgg aagttatggt gaaaatggta
     2761 accaatccat tgcagctttc cagtatttag caggtgtttc tttcgatgca aaagctgctt
     2821 attctttcgg caatgccaaa gattctcaaa caaccggtgg atatgcttat atacaaccaa
     2881 atcctgatgt aacctgggaa acatccattc aaaagaacat cggattggat gcacgtttgc
     2941 tgaattcacg tttaagtgtt gcaatcgatg tgtacgacaa attcaccaaa gactggttgc
     3001 ttcaagctcc cggattgggt actattggta ccggagttcc ttttataaat ggtggtgata
     3061 tcgataataa aggtattgaa gttgctcttg gatggaatga ccgcattggt aaagatttta
     3121 cttatggtat caatgttaac ggagcttata atcataatga agttacgcgt attgcaaata
     3181 cagaaggtgt tatacatggt actgtcaacg tgttaagtca gggaacaggt gaaatgtatc
     3241 gtgtacaagt tggatatcca atcggatatt tctatggata tcacacttcc ggaatttttc
     3301 agaatcaggc agaaatcgat gcatggaaag cagccggaaa cggtgttctt caatcaaaag
     3361 ttcaacccgg agatgttaaa ttctcggata ttagtggacc tgatggtaaa ccagatggct
     3421 cgattgacga aaacgacaaa ggtatgattg gtgatcctaa tcctgatttt acagcaggtg
     3481 gaagtattaa tttaggctac aagggttttg attttagtgt tacactttac ggagcatttg
     3541 ggcaacaaat tgcaaaatcg tatcgtaaat ttggcgacgg tcgtcacgaa aattatacca
     3601 ctgaagtata tgaacgctgg catggtgaag gaacttcgaa caaatggccc cgactgacag
     3661 ccggtacaaa tgccaattac atgaatatct ccgaccttta tatcgaagat gcagattttg
     3721 tgaaaattca aaacttaacc attggttatg acttcaaaaa actcttcaag agcttaccga
     3781 tgtcacaggc cagattgtat ttttcggcac aaaatctatt cacctttacc ggttactcag
     3841 gtatggatcc tgaagttggt tactctccac aagatacaga tggaacaact caaaattggg
     3901 ctaaaggaat tgacctcggt ttctatccgc aaccaagaac atatttagtt ggcgtaaatc
     3961 ttaaatttta atagaagatt aatatgaaaa aaataatata tttagcgata attacagcaa
     4021 ccgtgctttt taccggttgt gagagttttc ttgacacaaa ggacctgact cacaaaaata
     4081 cctcgaacta tccgcaaaca gttacggatg cgcaacaagt aattgcaggt atttataaca
     4141 atttgaatat aacacatgcc agccctgcat cttcattttt attcatttca gagcttgctt
     4201 ctgatgaccg ttttggtggt ggtggcaaac aagatttttc ttttcagtca aaagacttgt
     4261 tgatgcaagc tggttccaat gaatgtgaac aattttggaa agaccgttat gcaggcattt
     4321 tccgcgcaaa cacagccatc gaaactttgg caaattgctc cggatacgaa agcgataacc
     4381 agaaaaatca aatgataggt gaagcatatt tcatgcgtgc tttatattat tatgaactgg
     4441 cttctgaatt cgagaatatt cctttagtaa ttacatcggc ttcgggcaat cctgcacagg
     4501 ctactccgga tcaaacatgg ggacagattt gtgctgacct tttgaaagca attgaattga
     4561 tgccggataa taaggcaaat acttttgttg ctgctggtca tgccgacaat tgggctgctg
     4621 aagctttatt gggacgtgct ttcttattct ataccggctt ttatgaaaaa gctgctgtaa
     4681 ctcttcctga tggaacaagt ttagataaag ctgctgtagt tgcaaaaatt gatgattgcg
     4741 taaataattc cggttattca ctggttcctg atttccgtaa cctctgggct tattcaaaca
     4801 gacttacaaa agaagactac acatatacaa aaggacaagg tttgaaatgg gtagaagacg
     4861 ataacggtat caacccggaa tctatgtttg ctatcaaatt ctcaaaattc gcctcttggg
     4921 gaacaacaat tggttattcc aatggtaatg cactttattt tggagtacgt ggtggtcagg
     4981 atttagctaa aactttcccg tttggtcagg gttggggttg tggtccggta aatccggcat
     5041 tatggcagga atgggctgct gctgaaccta ctgatattcg tcgtaaagct acaatttgtg
     5101 agataccggc tgaattacct aattatacaa aaggagccgc atcttggaat gactttattc
     5161 aggaaactga ctattatgct aaaaaaactg ctcccataag ttcctttaaa gctgatggcg
     5221 taaaatgctg ggaaactttt gagcaagaca tgtatggtta tacatctgta aacttccaac
     5281 tttcgaatat tcatgatttg gtacttatcc gttttgcaga tgttcttttg atgcaatctg
     5341 aattaaaagg agatgcaaca ggaatgaata aggttcgtgc ccgtgttgga ttaccagctg
     5401 tcgcttatag tttagctgcg ctgcaaaaag aacgtcgttg ggaatttgcc tgcgaaggaa
     5461 ttcgttggaa cgatatgcgt cgttggcaca ttgctgaagc tgctttgggt actcagatag
     5521 gccagaatat ttatttacag ggagctgcaa ataaaaatac aaatcagggc gcaggatatg
     5581 tagcacgtta taaagctaca cgtggtttct tcccaatacc tgaaaaccag attgcattgt
     5641 ctaatggtac attgaagcaa aatgctggct ggggtactgc tgatactaat tatactggct
     5701 ggaaataatt tttgtaaaat aaacaaataa aaaatagaga ttatgaataa aatattttat
     5761 ataatatctg cctttattct cgtagcactc ttctctgcct gtgaaccgat tgtaaatccc
     5821 gtagatatgg gaactataat tacttctgca gacgaacttc aggctacggt aactcctgta
     5881 atggatggaa cgaagaagac aaataaagta atagttagtt gtacaagtcc ggttagttgc
     5941 caatggactg acggtgttaa aacttacact tcgaataata cagaaatgat attatttgtt
     6001 cctggtgaca tcgaaattac attgaaagct atggctgctg atggtactgt cttaactaag
     6061 aaatttacca cgaaagttga tgaaattaaa ttccctgttg aacctcaata tggttatttc
     6121 tgcggttcag gtgtaaaaac ctgggtttgg gctgttgata acacatacac aggaccacaa
     6181 agaatatggg gtaatggaaa tagtattagt tcaggagttc ctaattggtg gggcaaatct
     6241 gctgctgatg ctgaaccgga tattgatctt actgcaaaaa tgacatttac attgaatggc
     6301 ttaaaatttt caagaactga aagaggagtg actactaccg gaaacttttc atttgatatg
     6361 ggttctcata aagcatcaac aagtataggc tcagttacct ttataggaac aacaattcct
     6421 catggaattg ctcaaaataa tggtcaaaaa gtagtttata cttttgatat caagaaactt
     6481 accaacgatg aaatgatttt aatgtatcct actgatcctt ttactcctgg aaatgattct
     6541 gaaggttggt tctggttatt taaacgagaa ggctttacct attaaaaagt tcttttcgtt
     6601 atttaaacta tttaatatgt aagaatggac ttaaaattcc gttcttacat attgaaaaca
     6661 tttcaatata ataaatctaa caatttaatt ataaagaaga cgggtttttg cccgcattct
     6721 ttttgttctt taataatttt gaatgaatta tctacaaaaa aaatatcctg atttaaatgt
     6781 aactgctgaa atagccattg ttgagcctca tcataaagtg aaagaaatgc aggctatcat
     6841 gcatttagat aaaccggaag gaagtttcag ccaacagata caatcattcc gtacggctat
     6901 agtaaaggtt ttatcagaaa gagaattaca acatgcttct gtgttttcgg caagatggtt
     6961 tttgaccgat ccggctaatc aacaggtaca aatttcagca gagatggctg atgttttaga
     7021 ttgtccggta tgttatatca agcaaccgtt gctcgatgga agtaaaatcg ctctgtggat
     7081 gcagcttcat acagatatga aagttggtaa tgacgggctg tcatttcatg aacacaacaa
     7141 ttaccgccat tattacacta cagataactc tataaataat ggcaacgaaa gcgtaaattc
     7201 gtatgaacaa acaaaacaat tactggaagt atatgaaaaa caattatcag acagagaatg
     7261 taccattgaa agagactgtc tgagaacctg gttttttgta cgtgatgttg atgtaaatta
     7321 tcagggtgtg gttgaagccc gcaaagacaa cttccgacta aacggattga ctgaaaacac
     7381 acattatatt gccagtacag gcattgaagg aagtaccggc gatccggaca caaaagttgt
     7441 aatggatacg tatgctgtta aagggctgga tgaaggtcag gtgaaatttc tttatgcaaa
     7501 agaaaatcta agtcctacat acgattacgg cgttacattt gagcgtggag taagtgttga
     7561 ttttggcgat cgtaggaaag tttacctcag cggtacagcc agtattaatc atgagggaac
     7621 tatcttgcat cccggtgacg tggagaaaca ggtttacaga atgtgggaaa atgttgaagc
     7681 gcttttgcag gaagccgaat gctcttttga tgatctcatg cagctgattg tttatttgcg
     7741 tgatattgcc gattatcaat ctgtaaaaag aatgtttgat gagaaatttg ctgatacgcc
     7801 taaattaata gtacttgcac cgatatgcag accgggttgg ctggtagaaa tggaatgtat
     7861 agctgcaaaa ccaatcagca ataataattt cagagatttt tgaaaaggaa atacctgatt
     7921 atgtacccga ttttttcttc ttttcagaat ttattgattt aattattttt ttggaagtct
     7981 gcacaataat ttgttccttt gtgagcaggg attgacgtta agtatatgaa cagattccgt
     8041 tttgttgacc ttttttttag tgaaaagtta tgaataaaat cagtaggata ttttctttac
     8101 gcgctttttt ttcagtattg tttggctttg tcgtgtttct gttttttctg cggtattacc
     8161 agtttcattt acattacgag gaacaattac aactatttct ctttacttcg gattattgga
     8221 gccaactaat aagtaaaccc ggaggtttgg ctgattatat tggcacattt tttactcaat
     8281 tctattatgt ggaacgactg ggggcgttga taattgcctt attactcgtt ttattgcaat
     8341 cacaggttca ttcccttacc aaaaagatat ctaaaaacga aagttttttt ccactgacat
     8401 tcataccctc cattctcatt tttgcattac tctgcaatga aaatttcctg ctaagtggtt
     8461 taatagctct gatgttaacg ttggcggctg ttcggattta tacgttgata aaaaatcccg
     8521 ttgtccggat tatcggcgca attattgttg ctcccgttct tttctggttt gccggtggtg
     8581 tcttttggat ttgcagcttg ctttgtctga ttctggagtg gggctatttt aagcaactat
     8641 cgaaattaca gtggacaatt ttaatcttcg gtctgttggt tacagcatta tctcccgttg
     8701 tattcaaaca tttatttgtt cttcagtatc cgttatcccg tctctggtgg ggattatgct
     8761 acagccgtta tccggttgtt tctccgtatc cgctgctggt tgcctggttg tctgttccac
     8821 ttttagtcgt agctttcaga tttttacccg tgataaaaaa gcaatccgtt aatatcgccg
     8881 tgctgctgag tttaagtttg attgttgcca tctttgccgc atattttatc cgaaaatcgg
     8941 ctgatttcaa aaaagaagag attatggctt atgattatta tgcccgaacc caaaactggg
     9001 acaaaataat cgcactggcc aatcataaag atcccgattc acctttatcg gtagcatgtc
     9061 tgaatctggc gttgagcaag accggtaaat tgggcgacag catgttccgt tattttcaaa
     9121 atggtcccga aggattattg ccgacctttc agcgtgattt tacatcaccg tttatttcag
     9181 gcgaaatata ttatcacctg ggattcctga atacctccat gcggtataat tttgaagcga
     9241 tggaagcgat tccggattac aagaaaagtg cgcgtgccgt gaaaagaatg gcggaagtga
     9301 atttgttgaa cggagaatat aaagttgcag ccaagtacct gagttatttg caaagcacat
     9361 tattttatcg gtcatgggct gatgaaaccc ggaagtgcat tgataatccc aagttgattg
     9421 agcaaaacaa agaatggtct gccttgaaga agtaccggct caccgaagat tttcttttca
     9481 gcgagcagga taaagatcaa atgctgggaa tcttatttag tcatgccacc accaataaaa
     9541 tggcgtatga atacctgatg gcttatacct tgctgacaaa agacattgaa catttcgtga
     9601 agtattttcc gcttggaaaa agtctggact atcaggaaat tccatctcat tttcaggaag
     9661 cgttgattct tttttggtcc actcagtcgg ccgatttcga tcagacgccc tggcccatca
     9721 ccgatccggt taagcagtat ttggtgagtt atgcaaaagc ctataacgcc aatgggagag
     9781 aaaatgccga tccacgctcc atggctgact tttctcaaac ctactggtat tattatcatt
     9841 tcagaaaata acccttattc gtctttttat gaataaatac agcagctata tattaatttt
     9901 tttactgact ttgctggttg gttgtaacga aagtgttaag tcacccaaac tgattgatac
     9961 ttttcccgcc atttatcccg attatacctt tgtgactgtt ccggcatcta tcgcgccctt
    10021 aaattttacg ataaaatcgg tgaagtacga cgtgattgac gttgtggtaa cgggaaaggt
    10081 gaacggcacc ctgcacgtac agagtgatga aatcattcag tttccggaga aaaaatggca
    10141 taacctgctt gaaagcaata aaggttctga cattcaggtg ctggttagtc tgaaatctga
    10201 tggcgtgtgg aagcagtata aaacgtttcc ggtacatatc agccctaatc ccattgatta
    10261 tggattggtg taccgaatga ttgcgccggg ctatgaagtt tacagcaaga tgggcattta
    10321 tcagcgaaat ctttctgact ttgatcaaaa acccattgtc gaaaatacgt tgattccggg
    10381 ttcctgcgtc aactgtcatg ctttcagtaa aaataatcct tcgttcatga gtcttcacgt
    10441 gcgcggcacg cacggcgcga caatgctgca ggtgaacggg aaaatgcaaa tgctgaacac
    10501 gaaaaccgac agcaccattt cttcgtgcgt ttacccgtac tggcatcctt cgggaaaata
    10561 catcgcttac tctaccaata aaaccaaaca agcttttcat gtggtgcgga atgaacgggt
    10621 cgaagtgttt gatgaagcct cggatgtggt ggtttacgat gtcaactcca accaactgct
    10681 gacctgtgat gccttaaaaa cgagcgcttt cgaaacattt ccggtcttct cggctgatgg
    10741 tcgcgcgctg tatttttgtt cttcaaagcc ttatgacatt cagtcggatt acaagaaaat
    10801 aaaatatagc ctgtgtaaaa ttgattttaa tcccgacaat ggaaccttcg gcaccaaagt
    10861 ggataccctg gtttcgtcct ccaaactcaa taaaagtgta tcctttccgc gtccttcgtt
    10921 tgacggaaaa tacatcatgt tcaccttgtc cgattatggc aatttctcca tctggcaccg
    10981 cgaagctgat ctgtacctgc tgactttaag caccggcgcg ttccgtccca tcagcgaagt
    11041 gaacagtgct gataccgaaa gttttcacga ctggaccacc aattcacgct ggtttgtgtt
    11101 cagcagcagg cgaggggatg ggttgtacac gcgcctttat ctggcaagta tggatgaaaa
    11161 cggaaagata ggcaaaccat ttttgctccc gcaaaaagac ccccggtttt acgatgtctc
    11221 cctgttttca tacaatgttc ccgaatttat aaccgctcct gtcaaagtcg atgtcagcga
    11281 atttgaacgc aaagcagtaa g
//
LOCUS       DAOPZK010000064        10600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_7234_length_10600_cov_14.037364, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000064 DAOPZK010000000
VERSION     DAOPZK010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7234_length_10600_cov_14.037364"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..99
                     /locus_tag="PKH68_05355"
     CDS             7..99
                     /locus_tag="PKH68_05355"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12397.1"
                     /translation="MGYRYGLFNYATKTTGGYVDFDYFNINYNN"
     gene            153..1832
                     /locus_tag="PKH68_05360"
     CDS             153..1832
                     /locus_tag="PKH68_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNX12398.1"
                     /translation="MTLNPLMEMNAQRKSATNNPIFTNYVYQGEDQVYENNPLKPDEF
                     YNPILQGCYPDPSITRKGDDYYLVTSTFAMFPGVPIFHSKDLVNWTQIGHVLDRKSQL
                     KVQDTGISAGIYAPSIKYNANNETFYMITTQFAGDINNMVVKTKDPFQGWSEVYKLKF
                     NGIDPSLFFDDNGKAYVVHNDAPDRGKELYNGHRVIKIWEYDVENDQVIAGTDKIIVD
                     GGVDISRKPIWIEAPHIYKKNGIYYLMCAEGGTGDWHSEVIFTSENPMGPYVPAPNNP
                     ILSQRYLNSNRANKVDWAGHADLVEGTDGKHYGVFLAIRPNEKGLVNTGRETFILPVD
                     WSGKYPVFENGLVPMEPKLKMPKGVENKTGTEGFFPNGNFTFTDNFTAPKLDYRWIGM
                     RGPREDFITTSKNGLNIKPFAVSIKAVAPVSALFQRQQHQHFTATTTMKFTPKSENEL
                     AGLTCYQSERFNYVFGITKKDKDFYILLEKTEKGQSKIIASAKIDVKMPVQLQVTAKG
                     DEYKFNYSVDGVNFTNLGGIVSGDILSTNVAGGFTGSLIGLYATSANDAKP"
     gene            1896..3113
                     /locus_tag="PKH68_05365"
     CDS             1896..3113
                     /locus_tag="PKH68_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNX12399.1"
                     /translation="MYKRNFITGIILLLFLSVSVNGQNNIKVTKLKALKDAYTGKFLI
                     GTASDLRGFSDAELANIKMHYNAITPENSMKPQSLHPSMDKYNFGIADSLVDWSTKNG
                     LKVWGHTLLWHAQTAPWFFQANPELEETPRPRPAFQPNQPRAPRDPNAPRPSMAEMWL
                     RSVNGPLACRDVALERLKNHIMTVAGRYKGRVIGWDVANESIEDGGDGTTENLRNFSW
                     YKVVGPDVLTLAFKWAREADPDAELYYNDYNIEQGAVENKGKHASSMLLLKRLIAEGA
                     PITGVGIQGHWHLDTNLEDVEKAIENYASLGLRVAITELDVTATGNNSGAFDVNTGRR
                     EIPAENYLKQAEVYKKLFEIFMRHSDVIDRVTLWGVSDTRSWRRGQDALLFDGELQPK
                     PAFHAVIEAGKKK"
     gene            3529..7359
                     /locus_tag="PKH68_05370"
     CDS             3529..7359
                     /locus_tag="PKH68_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNX12400.1"
                     /translation="MKSKLSRVGLLIICFFSFLVFNSFREKTAKPIYLNTSYSFRERA
                     VDLVSRMTTDEKISQLGNTMPPIPRLGVNHYDVWGEALHGVMGRNNNSGMTATSFPNS
                     VAVGATWDPELIKREASVISDEARGFNHDLIFTLTYWSPVIEPARDPRWGRTAETFGE
                     DPFLVSEIGKGFIRGMMGNDPVYLKTVPCGKHYLANNSEFNRHTGSSNMDDRDMREFY
                     LAPYRKLIREYKLPSIMTAYNAVNGVPVSASRFLVDTIARKTYGLDGYVTGDCGAIDD
                     IVRGHFYTKSFEEAAAMGLKSGVDSDCGGVYQNHIQGALKNGLLTIGDIDRALINLFT
                     IRMRLGEFDPKAKVPFAGIKPGIINDPSHHNLAIEIAEKTPVLLKNNKLAQSGENALP
                     INLTKINKIAVLGPQADKVELGDYSGPVEPELRITPLQGIRSYIANNNLSTEVITRSG
                     GNTDRRTDFFSMTGFSTISDGKVKMDFDATKFDEAANGVIASYRFGQNVLQGIKDGDW
                     TLYKNVDISNVDSIRLNTGVSGDGGILEVRVGSASGNILASQEIKPLQNRGNLRNSGR
                     NQIIPVKINNLGISGPQSLVLVYKEAPGSGIDQETLDMAASADVALIFVGTDQSTGRE
                     ESDRFSLKLPGNQNELIQAVSAVNPNTIIVMQTMGMVEVEQFRNLPNVSGIIWTGYNG
                     QAQGEAMAKILFGDVNPGGKLNVTWHKSLSDLPDLNNYTLRGDGSNGRTYWYYNKDVS
                     YEFGFGMSYTTFEYSNFRINKTEITPNDKVTVSVDITNSGKMDGDEVVQIYVKTPDSP
                     SSLERPVKRLKGFKRVTIPRGQSKTVSIEIDCADLWFWDAENDKITFDQGKYIFEIGA
                     SSKDIRGSVQANLSGTYKPVLTTVVAESDLSVLKPGKTIETSLSAALSDDSFLDISKA
                     KVSYRCNNPSVAAVDANGKVTALGVGVATIFADVTVDGTTVSGSYPIKVMPDLTPKSI
                     SVNGKPVPDFSKDTKAYSYLLDKKSKIPEVKAAAMFPGIRVDIEQAESVPGTAIVRMI
                     DDVTSEINTYYLNFDVKSAGDEFNGNTLGNQWQWIREDPLTNSLSEKSGSLTITSQPG
                     DVSERTNNAKNILLQSTNNDWTIETKLTASRVPSQPENAGILVYQDDDNFVKLMFRAV
                     VKTSQMGRPGAPVQAGTIDLMIEENGISKSFATFNVPNPVTDGNPLILRLEKKGSLYT
                     AYYSLDGNKFDMLGSAVALLKNIRSGLIACDGIITQYMKSIFWFDSDTTKPDSPFNVS
                     FDYFRITNNGLKTK"
     gene            7371..8525
                     /locus_tag="PKH68_05375"
     CDS             7371..8525
                     /locus_tag="PKH68_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX12401.1"
                     /translation="MKKLNSFLFLTFFAGTFFGFMQINAQVVEDFKTSKTTQIGKNFP
                     QVNSEGRVRVQISAPDAKLVQLDIGAVKYDLKKDENGVWTGESAPQDEGFHYYQLNID
                     GASVPDPGSLYYYGASRWGSGIEVPAHDQDFYALKNVPHGQVREHIYYSEVNKSMRRC
                     FVYTPPGYDKNTKKRYPVLYLQHGGGENETGWSSQGHANLIMDNLIAEGKAKPFIIVM
                     DNGTWTMSGPRRTEGGTWPPTGWADGFMNTLLKDIVPMIDASYRTIANQKNRAMAGLS
                     MGGMQTRVITLANPDVFSQVGMFSGGSISPDDVNKAPGFREKVKLLFVSYGSYELDNP
                     RTNMNGGPKENTDKLKEMGMNTHFYVSPKTAHEWQSWRRSLYQFAQLLFK"
     gene            8649..10211
                     /locus_tag="PKH68_05380"
     CDS             8649..10211
                     /locus_tag="PKH68_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNX12402.1"
                     /translation="MKIKYLLFLLLLPGIFSYGQLRDAQVKVEGGIIQGVETDGMTVF
                     KGVPFAAPPVGELRWKAPQPVIGWEGIKKTVEFAPAPMQAGNPASGKSEDCLYLNIWT
                     PAKSSKDKLPVLVWIYGGGFSFGSTSDPVHNGENLAKKGVVVVSIAYRVGQLGFLAHP
                     ELSAENPNGVSGNYGLLDQIAGLKWVQNNIAAFGGDPHKVTIFGESAGGISVSMLCAS
                     PLTKGLFQGAISQSGGSFGPVRMTTYPGENMKTLSMAEADGVDYVKKMGVSSISDLRQ
                     LSAEKLIPQGWSMPGGWPIVDGYAIPDDQFALYEKGTYNDIPVLIGYNSDEGLSFPTG
                     RTKEEFIENVQKRFGPHAQALLKAYPLGENNIPRSARNLMRDAAFGWHTWSWARLQAK
                     TGNSKVYYYYFDQHAQQPKESPFYDYGTPHGVDVPFVFMNLDPNNGRTTESDLLISEI
                     MGTYWTNFAKYGNPNGNGLTNWPAFTNEKPVVMYLGPGAYLGKVPDEEALNVLDNYYT
                     WRRSAEGKEWAK"
     gene            10306..>10600
                     /locus_tag="PKH68_05385"
     CDS             10306..>10600
                     /locus_tag="PKH68_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12403.1"
                     /translation="MRIRLKNTLFLFAGMLIGILLMGCSGTKSLSDKNSVWVGSWSTA
                     PQLVEPHNMPPAPGLTDNTIRQIVRVSIGGDMVRLRFSNAFSKSPVEMKSVAIA"
BASE COUNT         3176 a         2183 c         2448 g         2793 t
ORIGIN      
        1 cactttatgg gctatcgtta cggtttgttc aattatgcca ctaaaaccac aggcggttac
       61 gtagatttcg actatttcaa catcaattac aacaactaaa tatcatgaga aagattcaaa
      121 aaaacttatt tctgcttagc ttgattgggt taatgaccct gaatccattg atggaaatga
      181 atgcgcaaag aaagtcagca actaacaatc cgattttcac taactatgtc tatcagggtg
      241 aagatcaggt gtatgaaaat aatccgctga agccggatga attctacaat cccattttgc
      301 agggctgtta tcccgacccg tccattaccc gaaaaggcga tgattattac ctggttacat
      361 ctacttttgc catgttcccg ggagtgccaa ttttccactc caaagatttg gtgaactgga
      421 cacaaattgg tcatgtgctc gacagaaaat cgcagctgaa agtgcaggat acaggcatca
      481 gtgccggaat ttacgctcct tccatcaaat acaatgctaa taatgaaacg ttctacatga
      541 ttaccacgca gtttgccggc gacatcaaca acatggtggt aaaaacaaaa gatccgtttc
      601 agggctggag tgaagtttac aagctaaaat tcaacggaat tgacccgtcg ctcttcttcg
      661 acgacaacgg aaaagcctac gtggttcaca acgatgcgcc ggaccgtgga aaagaactct
      721 acaacggtca ccgcgtgatt aaaatctggg aatacgatgt ggaaaatgac caggttattg
      781 ccggaacgga taaaattatc gtggatggcg gtgtggatat ttccagaaaa ccaatctgga
      841 tcgaagctcc gcacatttac aaaaaaaacg gcatttacta cctgatgtgt gccgaaggtg
      901 gaacaggtga ttggcacagc gaagtgattt ttacgagcga aaatccgatg ggaccttacg
      961 ttcctgctcc gaacaacccg attttatcgc aacgctacct caactcaaac cgcgccaaca
     1021 aggtggattg ggcaggtcat gccgatctgg tggaaggtac tgacggaaaa cattacggtg
     1081 tatttctggc cattcgcccg aatgaaaaag gactggtaaa caccggtcgt gaaacgttta
     1141 tcctccccgt ggactggagc ggcaaatatc ccgtgttcga aaacggactg gtacccatgg
     1201 aaccgaagtt gaaaatgccg aaaggtgtgg agaataaaac cggaacggaa ggattttttc
     1261 ccaacggaaa tttcactttt accgacaatt tcacagcgcc gaaactcgat taccgctgga
     1321 ttgggatgag aggtccgcgt gaagatttca tcaccacctc taaaaacgga ttgaacatca
     1381 aaccttttgc tgtaagcatc aaagcagttg caccggtttc tgcacttttt cagcgccaac
     1441 agcaccagca ctttaccgcc acgactacga tgaagtttac tccaaaatcg gaaaatgaac
     1501 tggccggatt gacctgctac caaagcgaac gattcaatta tgtgtttggt ataacgaaaa
     1561 aagacaaaga cttttacatt ctgcttgaaa aaacggaaaa aggacaatcc aaaatcattg
     1621 caagcgcaaa aattgatgtg aaaatgccag ttcagctgca ggtgacagcc aaaggtgacg
     1681 agtataaatt caattattct gttgacggcg tgaatttcac gaatctgggt ggcattgtgt
     1741 caggcgatat cctctcaacc aacgtagccg gcggatttac cggaagttta atcggactgt
     1801 atgctacaag tgccaatgat gctaagcctt aacaagatat gagataaaaa agtgtgatta
     1861 ttcggaacta atttaaaatt ctaaaaaata aaattatgta caaaagaaac tttattacag
     1921 gcattattct gctgttgttt ctgtctgtaa gtgtaaatgg gcagaacaat atcaaagtaa
     1981 caaaattaaa agcgttgaaa gatgcttata caggaaaatt cctgattgga actgcaagtg
     2041 atttgagggg attctctgat gctgaattgg caaatatcaa gatgcattac aacgctatca
     2101 ctcctgaaaa ctccatgaaa ccgcagtcgc tccatccttc aatggataaa tacaactttg
     2161 gaattgctga ttctttggtt gactggtcca cgaaaaatgg tttgaaggtt tggggacaca
     2221 cgttgctgtg gcatgctcaa acggcaccct ggttttttca ggccaatcct gaactggaag
     2281 aaacgccgag accaaggcct gcttttcagc cgaatcagcc cagagctcca cgggatccca
     2341 atgctccccg ccccagcatg gctgaaatgt ggttgcgaag tgtcaacgga cctctggcat
     2401 gtcgtgacgt tgctttggaa cgtttgaaaa accacattat gacggtagcc ggacgttata
     2461 aaggccgcgt aatcggatgg gatgtggcta atgaatcgat agaagacgga ggtgatggta
     2521 caactgaaaa tttgcgtaat ttcagctggt acaaggtcgt tggacccgat gtgctgaccc
     2581 tggctttcaa atgggcacgc gaggcagatc ctgatgcaga attatattac aacgattaca
     2641 atattgagca gggcgcagta gaaaacaaag gcaaacacgc cagttcgatg ttacttctca
     2701 aacgtcttat tgcagaaggt gctccaatta ccggcgtggg cattcaggga cactggcacc
     2761 tggacacaaa tctggaagat gttgaaaaag ccatagagaa ttacgcatcc cttggattga
     2821 gggttgcgat taccgagctt gatgttacag caacaggtaa caacagtggc gccttcgacg
     2881 taaatacagg cagaagagaa atcccggctg aaaactacct gaaacaggct gaagtttaca
     2941 aaaagttgtt tgagattttt atgcgtcatt cagacgtgat tgaccgcgta acgttatggg
     3001 gagtcagcga tacccgttcg tggcgtcgtg gacaggatgc actgttgttt gatggagagt
     3061 tgcaacccaa accggcattt catgctgtta ttgaagcagg taaaaaaaaa taattgctgt
     3121 ttttcaaaaa cacaattctt tgagtccact ccgaaaagtc ataatttata aaaatctaag
     3181 atttttatcc tttgtgtcga aattagatgt gctgataatc aattagatat aaaattagtt
     3241 tctttgttta acttggtgtt ttagtgtctt tgtggcaaaa acaaggtttt cggagcagac
     3301 tcattctttc aaaaaaagaa ttttccccgg attcattatg agttgataat gaatccggga
     3361 atattgcttt gaacattgaa atattaggac tattgatagg gagtttagca aatcaatcct
     3421 tatttttttt tcatcataag ttgtatacct cctcattcac tgtaggttac attatattta
     3481 cctaatcaaa aaatcagatt gttctcagta ctttaatacg taaatacaat gaaaagtaaa
     3541 ttatcccgag tgggtttatt gattatttgt tttttttcat ttttggtctt taattctttc
     3601 cgggaaaaaa ctgcaaaacc gatttacctc aacacatcct attcattcag ggaacgtgcc
     3661 gtagaccttg tttcacgcat gacgacggat gaaaaaatca gccagttggg aaatacgatg
     3721 ccacccattc cccgcctggg agtaaaccat tacgatgttt ggggagaagc cttacacgga
     3781 gtgatgggtc gaaacaacaa cagcggaatg accgctactt catttccaaa cagcgtagca
     3841 gtaggcgcta cctgggatcc agagctgatt aaacgtgaag cctcagttat ttctgatgag
     3901 gcacgaggtt tcaatcacga tttgatattt acgcttactt attggtcacc ggtgattgag
     3961 cctgcgcgcg acccgagatg gggaagaaca gccgaaacgt tcggtgaaga cccgtttctt
     4021 gtttccgaaa taggcaaagg atttatccgg ggaatgatgg gaaatgatcc tgtttaccta
     4081 aaaactgttc cgtgcggaaa gcattatctg gcaaataatt ccgaattcaa ccgccacacc
     4141 ggcagttcga acatggacga cagggatatg cgggaattct atctggcacc ttaccgaaaa
     4201 ctcatccggg aatacaaact tccgtccatt atgactgcct acaatgctgt aaacggtgtt
     4261 cctgtttcgg ccagccggtt tttggtggat actattgccc gaaaaacata cggtttggat
     4321 ggttacgtga ccggcgactg tggtgccatc gacgacattg tacgagggca tttttatacg
     4381 aaaagttttg aagaagcagc tgccatgggc ttgaaatcgg gtgtggattc cgattgcggc
     4441 ggcgtttatc agaatcacat tcagggagca ttaaaaaatg gattactgac catcggcgat
     4501 attgaccgcg cactgatcaa tctttttacc atccgcatga ggttgggcga atttgatccg
     4561 aaagcgaaag ttccatttgc cggtataaaa ccgggtatta tcaatgaccc gtcgcaccat
     4621 aatttggcta ttgaaattgc cgaaaaaaca ccggtgcttt taaaaaacaa caaactggct
     4681 caatccggcg aaaatgcatt gccaattaat ctgacaaaga taaataaaat tgctgttttg
     4741 ggacctcagg cagataaggt ggagttgggc gattattccg gtccggtcga accggagttg
     4801 cgaattacac ctctccaggg cattcggagt tacattgcaa acaacaacct gagtacggaa
     4861 gttattaccc gttcgggtgg aaataccgac cgtcgcaccg actttttttc aatgaccggt
     4921 ttttctacca tttcagacgg aaaagttaag atggattttg acgcaacaaa atttgacgaa
     4981 gcggcaaacg gagtcatcgc ttcttaccgg tttgggcaaa acgttttgca gggaatcaag
     5041 gacggcgact ggactttata caaaaacgtg gatatatcaa atgtggattc catccggttg
     5101 aacacgggtg tttcgggtga tggcggtatt ctggaagtaa gagttggttc tgcctccggc
     5161 aatattctgg cttcgcagga aatcaaaccg ttgcaaaacc gtggaaattt aagaaattcg
     5221 ggtagaaatc aaattattcc ggtaaaaata aacaacctgg gaatttcagg accgcaatca
     5281 ttggttttgg tctataaaga agctcccggc tccggcattg atcaggaaac gcttgatatg
     5341 gcagcctcgg ccgatgtcgc attgattttt gtaggtactg atcaatccac aggtcgcgaa
     5401 gaatccgacc gtttttcact aaaacttccc ggaaaccaga atgaattaat tcaggctgta
     5461 tccgcagtca atcccaatac tatcattgtc atgcagacta tgggcatggt ggaagtggag
     5521 cagttcagaa atctgccaaa tgtctccggt attatatgga caggctacaa cggtcaggca
     5581 cagggcgagg ccatggctaa aattctcttc ggcgatgtaa atccgggtgg aaaactcaac
     5641 gttacgtggc ataaatccct cagcgatctg cctgatttga acaattatac gcttcgcggc
     5701 gatggctcta acggaagaac ttactggtac tacaacaagg atgtatccta cgaatttggc
     5761 tttggaatgt cctacaccac gtttgaatat agtaatttca gaatcaataa aacggaaatc
     5821 acacccaacg acaaagttac tgtcagcgtg gatattacca attccggaaa aatggatggc
     5881 gacgaagtgg tacagattta tgtgaaaact ccggacagtc cgtcatcgct ggagcgaccg
     5941 gtaaaaaggc tgaaaggctt caaacgggta accattcccc gcggacagtc caaaaccgtt
     6001 tccatcgaaa tcgactgtgc cgatctttgg ttttgggatg ctgaaaatga caaaattaca
     6061 tttgatcagg gaaaatatat tttcgaaatc ggagcatctt ccaaagacat ccgtggaagt
     6121 gtgcaggcta atctgagcgg tacgtacaaa cctgtgctga caaccgttgt ggcagaaagc
     6181 gacctgtctg tactgaaacc cggaaaaaca atagagactt ctctttcagc agccttgtcg
     6241 gatgatagtt ttttagatat ttccaaagca aaagtaagtt acaggtgcaa taatccttcg
     6301 gtcgctgctg tggatgcaaa cgggaaagta acagccttag gcgtaggtgt ggctaccatt
     6361 tttgcagatg taactgttga cgggacgacc gtttcgggca gttatccgat aaaagtgatg
     6421 cccgatctta ctccgaaatc aatctcggtg aatggaaaac cggtacctga tttttccaaa
     6481 gacaccaaag cttacagcta cctgctcgac aaaaaatcga aaattcctga ggttaaagct
     6541 gcagccatgt ttccgggaat tcgggtagat atcgaacaag cagaaagcgt acccggaaca
     6601 gccattgtaa ggatgattga cgatgttacc tcagaaataa acacctatta tttgaatttt
     6661 gatgtaaaat ccgccggtga tgaattcaac ggaaatacat taggcaatca atggcaatgg
     6721 attcgggaag atccgctgac aaattcgctt tctgaaaaat cgggttctct gactattacc
     6781 agccagcccg gagatgtgtc agaacgcacc aacaatgcca aaaacatcct cctgcaaagc
     6841 accaacaacg attggaccat cgaaacaaaa ctgactgcat cgagagtgcc ttcacagccc
     6901 gaaaatgcag gaattctggt ttatcaggac gatgataatt ttgtaaaact gatgttccgc
     6961 gctgttgtga aaacctctca gatgggcaga ccgggcgctc cggttcaagc tggaaccatc
     7021 gatctgatga tagaagaaaa cggtatttct aaatcatttg ccactttcaa tgtgccgaac
     7081 cctgtaacag atggcaatcc gctcattctc agactggaaa agaaaggaag tctctataca
     7141 gcctattatt cattggatgg aaacaagttt gacatgctcg gctcggcagt tgcattgctt
     7201 aaaaatatcc gctccggatt gatagcttgc gacggaatta ttacgcagta catgaaaagc
     7261 attttctggt tcgattcgga taccaccaag cccgattctc ctttcaatgt ctcgtttgat
     7321 tatttcagaa ttactaataa tggtttgaaa accaaataaa tacaaatatc atgaaaaaat
     7381 taaattcttt tctctttcta actttttttg ccggtacatt tttcggattc atgcagataa
     7441 atgcacaggt ggtggaagat ttcaagactt ccaaaacgac tcagattggt aaaaattttc
     7501 cgcaggtaaa ctctgaagga agagtgcgtg tgcaaatatc agcaccggat gccaaactgg
     7561 tgcagctcga tattggcgct gtcaaatacg atttgaaaaa ggatgaaaac ggagtctgga
     7621 ccggtgagtc agctcctcag gatgaaggtt ttcactatta ccagttaaac atcgacggtg
     7681 cttcggttcc cgatccgggc agtttgtact actacggtgc ttccagatgg ggcagcggaa
     7741 ttgaagttcc ggcacacgat caggattttt atgctttaaa aaatgtacct cacggacagg
     7801 ttcgggagca catttattac tccgaagtaa ataaatccat gcgtcgctgt tttgtttaca
     7861 ctcctcccgg atacgataaa aacacaaaga aaaggtatcc tgtgctctat cttcaacacg
     7921 gtggcggtga aaatgaaacc ggctggtcat cgcagggaca tgccaatctc atcatggaca
     7981 acctcattgc cgaaggcaaa gcaaaacctt tcatcatcgt aatggacaac ggcacctgga
     8041 caatgagtgg tccccgaaga accgaaggcg gcacctggcc acctacaggt tgggcggatg
     8101 gatttatgaa cacccttttg aaagacatcg tacctatgat tgatgcaagt taccgcacca
     8161 ttgccaatca gaaaaaccgc gctatggctg gtctttcgat gggtggcatg cagacccgcg
     8221 tgattacttt ggctaacccg gatgtgttct cacaagtagg catgttcagc ggcggaagca
     8281 tcagtccgga tgatgtgaat aaagcgcccg gtttcaggga aaaagtgaaa ctgctttttg
     8341 tgagctatgg cagctacgag ctggataatc cgagaacgaa catgaacgga gggccgaaag
     8401 aaaatacaga taaactgaaa gaaatgggta tgaatacgca tttctatgtt tctccgaaaa
     8461 cagctcacga atggcagagc tggcgcagga gtttgtatca gtttgctcag ttgctgttta
     8521 agtagacatg acactaaaaa ataatttata atcagatgaa acagccattt gagaaaaatc
     8581 tcaaacacaa tgagatgatt gatggcgtgt tccatgtgtc cttagtgtaa tatataaatt
     8641 ataaacacat gaaaataaaa tatttgttat tccttctttt attacccggc atcttttcgt
     8701 atggacaact tcgagatgct caggttaaag tggaaggtgg aattattcaa ggggttgaaa
     8761 ctgatggcat gactgttttc aaaggagttc cctttgctgc cccgccagtg ggagaacttc
     8821 gctggaaagc tcctcagccc gtcattggat gggaaggaat aaaaaagacc gtggaatttg
     8881 ctccggcacc tatgcaagca ggtaatcccg cttccggaaa aagtgaagac tgtttatatc
     8941 tgaacatctg gacaccggca aaatcctcaa aagataaact accggtttta gtctggattt
     9001 atggtggagg attcagcttt ggatcaacct cagatccggt tcacaatggt gaaaatttgg
     9061 caaaaaaagg agtagttgta gtcagcatag cttatcgtgt tggacaactt ggctttttgg
     9121 cacatcccga attgagtgca gaaaatccaa atggagtttc cggtaactat ggcctgctcg
     9181 atcaaattgc cgggttgaaa tgggtacaga ataacattgc tgcttttgga ggtgatcccc
     9241 ataaagtgac aattttcggt gagtctgccg gcggtatttc tgtaagcatg ctgtgtgctt
     9301 cgcccttgac aaaaggttta ttccaaggtg ctatttcaca gagcggcggc tcatttgggc
     9361 ctgttcgaat gactacgtat cccggagaaa acatgaagac tttatcaatg gcagaagctg
     9421 atggtgtgga ttatgtaaaa aaaatgggag tatcatccat cagtgatcta cgtcagctca
     9481 gtgctgaaaa acttatcccg caagggtgga gcatgcctgg cggatggcct attgtggatg
     9541 gatatgctat ccctgatgat caatttgctc tttatgaaaa gggaacgtac aacgacattc
     9601 cagtactaat tggatacaat tcggatgaag gtctgagttt cccgacagga cgcaccaagg
     9661 aggaatttat tgaaaatgtg caaaaaaggt ttggtcctca tgcccaagct cttttgaaag
     9721 cttatccatt gggtgaaaac aacattcccc gttctgcacg caacttaatg cgtgatgccg
     9781 catttgggtg gcacacctgg agttgggcgc gccttcaagc aaaaaccgga aattcaaaag
     9841 tttattacta ttattttgat caacatgccc aacaacctaa agagtcacct ttttatgatt
     9901 atggaactcc gcatggtgtt gatgtgccgt ttgttttcat gaacttagat ccgaataatg
     9961 gaagaaccac tgaatctgat ttgttgattt cagaaatcat gggaacatat tggactaatt
    10021 ttgccaaata tggcaatccc aatggtaatg gtttaactaa ttggcctgca tttacaaatg
    10081 aaaaaccagt tgtgatgtat ctgggacccg gggcgtattt gggaaaagtt cctgatgaag
    10141 aagccctgaa tgtgttggac aactactata cttggaggcg gtcagcagaa gggaaagaat
    10201 gggcaaaata aatacttgaa agcatatgtt ttgcttgtat tttttcaaat agtaaaaatg
    10261 ccagacagtg aagttttcca ttcttcattg taaaaagaga aaaaaatgag aattagatta
    10321 aaaaatacgt tgtttttgtt tgccggaatg ctcatcggca ttttgttgat gggctgttcc
    10381 ggtacaaaat ccttgtcaga taaaaactca gtttgggttg gttcttggag tacggctccg
    10441 caattggtgg agccgcacaa catgcctcct gcacccggat taaccgacaa tacaatccgt
    10501 cagattgtcc gtgtttcgat aggcggtgat atggtcagat tgcgtttttc gaatgccttc
    10561 agcaaaagtc cggtggaaat gaagtcggtt gcaattgctc
//
LOCUS       DAOPZK010000065        10583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_7258_length_10583_cov_23.695004, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000065 DAOPZK010000000
VERSION     DAOPZK010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10583
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7258_length_10583_cov_23.695004"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKH68_05390"
     CDS             complement(<1..209)
                     /locus_tag="PKH68_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12404.1"
                     /translation="MLKFLFKGLIRDRSRSLLPVIVVMMGVMITVLLQSYMTGALGDS
                     IETTANFTTGHVKVMTRAYSKNMSQL"
     gene            complement(249..>1360)
                     /locus_tag="PKH68_05395"
     CDS             complement(249..>1360)
                     /locus_tag="PKH68_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNX12405.1"
                     /translation="NVFVLSISFVLIIFMQGLLEGWSKQAVTDSVAWEIAGGQYWSEK
                     YDPYDPFSLDSSAAVIPQSLMNDYNAHKIEPVLIASGSIYPAGRMTPVLMKGIRADQQ
                     LLALPTENMKADSTEIPVIIGAHMASQTGLKENDRVTLRWRDANGTFEAVDVRIARVF
                     KTTIPTTDTGIIWLPLEKMQKMMSQPGKATMLIKDTEIPVENIPDWKFRNKTELTKQT
                     IALVNAKSVGMAIFYFIFLFLALIAIFDTQTLSIFRRQREIGTYVALGMTPRQVQNFF
                     TLEGTMNALLAIGLGFVYGLPFFIYFAANGIAMPMDVSNFGLPIPDKMYPLFTPKLII
                     GTILFIFIITAIVSYLPARKISRMKPTEAIRGKVL"
     assembly_gap    1361..1460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1461..1669)
                     /locus_tag="PKH68_05400"
     CDS             complement(<1461..1669)
                     /locus_tag="PKH68_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12406.1"
                     /translation="MNKINKDKDRDKTNSGVIQTPGESSPFRGLGGFFLLKTAIKSLL
                     GNGLKTWLNVFVLSISFVLIIFMQG"
     gene            complement(1749..2507)
                     /locus_tag="PKH68_05405"
     CDS             complement(1749..2507)
                     /locus_tag="PKH68_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX12407.1"
                     /translation="MNTNKKLNNGVLPSTIEVGEDSIVEIKNLVKNFPIGNDFFTALK
                     GINLTFTEGEFAGLVGPSGSGKTTLLNIIGSLDVPSDGDVIVLSHSISNLTQKQAAQL
                     RKEKLGFIFQNYNLLPVYTVYENVEFPLLLLDISADERKKRILETLEWVGLSDKIRSR
                     PSQLSGGECQRVAIARAMVKRPSLVLADEPTANLDSENSHNVLQTMVKLNRELKTSFI
                     FATHDDKVIGYLRRIIRLEDGKVVDDRPQTPKGV"
     gene            complement(2586..3713)
                     /locus_tag="PKH68_05410"
     CDS             complement(2586..3713)
                     /locus_tag="PKH68_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12408.1"
                     /translation="MMLPGLEAVQAQEVNDSTEVTHAVTFSGQATVWEVTRFSNPLIW
                     QAGERFVPKLLGNFDIRKHSKLDFEASLHLNGALNFTSLDYSSKNGEISPYRVWLRYS
                     TDHWEVRAGLQKINFGTAKMFRPLMWFDQMDVRDPLRLTNGVYGILGKYFFKNNSNIW
                     LWTLAGNKKPKGWEYLGSKQWKPEVGGRLEVPLLQGEIALSTNFRSVELPVTGKSLQE
                     NRIGLDGKWDVGPGIWFESSITQLQKNDYNLPLYQDAWNVGADYTLNIGSGIGLTLEY
                     FRYHSGNDFLVNGTGINLLGSMITYPVSMLDNLSAVIFYAGGNYNLWFNYLSWSRTYD
                     NLSFYLMAFWNPEISLPLNTVLQGKNLFAGKGVQVMVSYNF"
     gene            4007..4468
                     /locus_tag="PKH68_05415"
     CDS             4007..4468
                     /locus_tag="PKH68_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNX12409.1"
                     /translation="MKKEYTFNELLHKAASYCSISEHCISDVNDKLKAWQVDEADRKK
                     IIDQLCEDDFINEKRFCKAFVRDKFHLNKWGKIKISMALREKGLSNELITAALKVIDE
                     GEYEELLASLLKSKQNTIKYEFEYEKQGKLYQFAQSRGFENQVIERVLRLI"
     gene            complement(4634..4807)
                     /locus_tag="PKH68_05420"
     CDS             complement(4634..4807)
                     /locus_tag="PKH68_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HNX12410.1"
                     /translation="MKELVAKIQEQIDAFAENAGLQVEKNNKAAGARARKASLELEKL
                     LKEFRKVSITASK"
     gene            complement(5176..5787)
                     /locus_tag="PKH68_05425"
     CDS             complement(5176..5787)
                     /locus_tag="PKH68_05425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNX12411.1"
                     /translation="MTDTFAHKAAGWDQPDKIKMSDKFAAELLKHVDLQPHWKALEIG
                     AGTGLVGLQLLPMLKSCVFEDTSEAMLAILKQKTDELPNVEIVHGEVTAYTQPDIDLV
                     FSNMAFHHITDIAATLQHLHTITKPGARVVVGDLRSEDGSFHHFEAVPHRGFDTDQLA
                     DIFHQAGFTVLMTKTYNTLHREVTPGNPRVYELFLLMAERRDS"
     gene            6089..8176
                     /locus_tag="PKH68_05430"
     CDS             6089..8176
                     /locus_tag="PKH68_05430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNX12412.1"
                     /translation="MKSTLKTILMAIALTAAVSACDTKKEDAPLIEKQVPVIENGRMT
                     PEILWSFGRVGNVEVSPDETKILYSVSYYSVEQNKSNSELFVMNTDGSGKKQITKTAN
                     KEIAAKWMSDSKHIAFLSNESGSMQLWTMKDDGTARTRLTDREGDISDFSFSPDERKV
                     LLVADVKYGKRTADIHPDLPKASGVIVNDLMYKHWDEWVQTVPHPFVADIEGGKLTNE
                     RDILKDEPYESPMKPFGGIEQLAWSPDGKTIAYTCRKKTGKEYALSTNSDIYFYDLET
                     GKSVNKTEGMMGYDQNPVFSPDGKWLAWESMEHDGYESDKSRLFVMDVKTGEKKDVSA
                     AFDQNSNGLKWSVDGQSIYFISCYHAVTQIYKADLKDGKIMPVTKGVHDYGTVIPMKN
                     QLIGVKQSMSKPDEIYAINASTGEEKELSFENKDILDKLEMGKVEERWITTTDNKQMH
                     TWVIYPPHFDPAKKYPTLLFCEGGPQSTVSQFWSYRWNFQIMAANDYIIVAPNRRGLP
                     GFGYEWLKQISGDYGGQNMKDYLSAIDAVAKEPFVDKDKLGCVGASYGGFSVYWLAGH
                     HQKRFKAFIAHDGMFNLPQQYLETEEKWFVNWDLGGAYWEKHNAIAQRSYANSPHLFV
                     DKWDTPILIIHGEKDYRILASQGMAAFDAAVLRGVPAEMLIYPDENHWVLKPQNGILW
                     QRTYFGWLDKWLK"
     gene            8460..9011
                     /locus_tag="PKH68_05435"
     CDS             8460..9011
                     /locus_tag="PKH68_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNX12413.1"
                     /translation="MDEQRLIAGCKRGEQWARREVYEKYASAMLSLCVRYVADYEAAR
                     DVLQDGFVKLFTRAEQYDGTGNFGGWIRQIFVNTALEYLRRQNRLETDAISEDLKERN
                     DNRNFSPGKLTADDLMECVAELPEKLRTVFNLYAIEGFSHSEIAAMTGIQESTSRSQF
                     LRARQLLQQKVSLLMKNDHAGKK"
     gene            8995..10371
                     /locus_tag="PKH68_05440"
     CDS             8995..10371
                     /locus_tag="PKH68_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNX12414.1"
                     /translation="MQEKNKHTNEPDAFSQWARQQLENHELPVDESLWQGIEQKLAPA
                     PKRRIPVWYWLAGGVAAVVALMLLIRPYYTTQTDKELAQTTLSREPVTKSAAVSSENP
                     ARLSGVPAEDDIMHPAAQADASGSAGLLNSQKVQQTNQSTPVKTAHAVSNQKKTFIAS
                     ASTFSTKSTSASTAINTINDNKRTVTDEKQIAAVADETAKKSDINPAKTEKQQITSLP
                     DLSDYPEAPADEPAKNKKKQHLLLAANVGAGSTLISNGSGLFLESGAMSDAALVNSTS
                     VRAEYAADIKEADYFNEVQHLPPVSFGLMAELPLSKSWSVESGLVYTYLESRFTRTAN
                     PTGTGHLKLHYLGLPLYLKAKLWHDPTWSIYLNAGPMIEKGLQSVYSQDIDYTWSITH
                     TDVHSRIEGLQWSLNTGLGVNYKIQKALSVFFEPKLTYYLDNNQPMSARTEQPLVFGV
                     NGGVRIEL"
BASE COUNT         3091 a         2229 c         2365 g         2798 t
ORIGIN      
        1 agttgcgaca tgtttttgct gtaagcgcgc gtcatcactt tcacgtgtcc ggtggtgaaa
       61 ttggccgtgg tttctatgct gtcgcccagg gcgccggtca tgtacgattg gagcaacacc
      121 gtaatcatca cccccatcat cactacaatg acgggcagca aactgcggct tcggtcgcgg
      181 ataagtcctt tgaataaaaa tttcagcata atattgcgat gaatgtgaaa cgtttgactt
      241 ctgtttagtt acaacacttt tccccggata gcttccgtag gcttcatgcg cgaaattttt
      301 cgggcaggca gataacttac gatggctgta atgatgaaaa tgaagaggat ggttccgata
      361 atgagtttcg gtgtgaacag cggatacatt ttatccggta tcggcaatcc gaaattgctc
      421 acatccatcg gcatggctat tccattggca gcaaaataaa tgaaaaacgg cagtccgtac
      481 acaaaaccca gcccgatagc cagcagtgca ttcatcgtcc cttcgagggt gaagaaattc
      541 tgcacctggc gcggcgtcat tcccaacgcc acatacgtac ctatttcgcg ctggcgacgg
      601 aaaatggaca atgtttgtgt gtcgaaaatg gctatgagcg ccagaaaaag aaatatgaag
      661 taaaaaatgg ccattcctac cgattttgca ttgacaagcg caatagtctg tttggtgagt
      721 tcggttttgt tcctaaattt ccagtccggg atattttcca cagggatttc tgtgtctttt
      781 atcagcatag tggctttgcc gggttgcgac atcatttttt gcattttttc caacggcagc
      841 cagattattc cagtgtcggt agtcggtatg gtggtcttga aaacccgtgc aatgcgaaca
      901 tcgacagctt caaaagtgcc gttggcatcg cgccagcgca gggtgacccg gtcgttttcc
      961 ttcaaaccgg tctgactggc catgtgagcg ccaatgatca caggaatttc ggtactgtcg
     1021 gctttcatgt tttcggtagg caacgccagc aactgctgat cggcacgaat acctttcatc
     1081 aatacgggcg tcatccggcc agcgggatag atgctccccg aagcgattaa caccggttca
     1141 atcttgtgtg cattataatc attcatcaat gattgaggaa taacggcggc actcgaatcg
     1201 agcgagaacg gatcatacgg atcgtatttt tcgctccagt attgtccgcc ggcaatttcc
     1261 caggctaccg agtcggtcac cgcctgctta ctccagcctt ccagcaatcc ctgcatgaaa
     1321 ataatcagta caaaggatat ggaaagcaca aaaacattca nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn aatccctgca tgaaaataat cagtacaaag gatatggaaa
     1501 gcacaaaaac attcaaccag gttttcagac cattgcccag tagacttttt atggcagttt
     1561 tgagaagaaa aaagccccct aaccccctaa agggggaact ttccccgggt gtttgaatga
     1621 cacctgagtt ggttttgtct ctgtctttgt ctttgtttat tttgttcata tttaagattt
     1681 tcagcattca actgtattct tttactctca gcaatttatt gcttttggca ggatggattg
     1741 ggactatctt ataccccttt aggggtctgg ggtctgtcat ccactacttt accgtcctcc
     1801 aatcggatga tgcggcgcag gtatccgatt actttgtcat cgtgagtggc aaaaataaaa
     1861 ctggttttca gttcccggtt cagttttacc atggtttgga gtacgttgtg tgaattttcg
     1921 gagtccagat tggccgtggg ttcatcagcc agcaccagcg acgggcgttt aaccatggcg
     1981 cgggctatgg ctacccgctg acattctcca cctgaaagct gcgacgggcg tgaacggatt
     2041 ttatccgata agcccaccca ttccaaggtt tccagtattc gttttttgcg ctcgtcggcg
     2101 cttatgtcca gcaataacag cggaaattcg acattttcgt acacggtgta cacgggcagc
     2161 aggttgtaat tctgaaagat aaatcccagt ttttctttcc ggagctgtgc ggcctgtttt
     2221 tgcgtgagat tactgatgga gtggctcagc acaatcacat cgccatcaga aggtacatcc
     2281 agcgaaccga taatgttgag cagagtggtt ttcccggaac cgctcggacc caccaatccg
     2341 gcaaattcgc cttcggtgaa ggtcagatta atgcctttca atgcggtgaa aaagtcattt
     2401 ccgataggga aattttttac cagatttttt atttcgacaa tagaatcttc cccaacctca
     2461 atagtggaag ggagaactcc gttattcaac tttttgttgg tattcataaa cgtaaattta
     2521 tatttcgtta aaaatattcc tgtataaatt ggcctgttcc cctccttcgg aggggttagg
     2581 ggaggctaaa aattgtaact caccatcacc tgcacacctt ttcctgcaaa cagatttttt
     2641 ccctgcaaaa ccgtatttaa tggtaaacta atctccggat tccagaatgc catgaggtaa
     2701 aaactcaggt tgtcgtacgt tcggctccag ctcaggtagt tgaaccataa attgtagttt
     2761 cctccggcgt aaaaaatcac agcactcagg ttatcgagca tgcttacagg gtaagttatc
     2821 atactcccga ggagattgat accggttcca ttaaccaaaa aatcatttcc ggagtgatac
     2881 ctgaaatatt cgagcgtaag accaattcca ctgccaatgt tcaacgtata atctgctccc
     2941 acattccagg catcctgata aagcggcaga ttgtaatcgt ttttttgcag ttgtgtgatg
     3001 ctggattcga accagatgcc gggtccgaca tcccatttac catccaaccc gatgcggttc
     3061 tcctgtaacg atttaccagt caccggcagt tccactgatc ggaaatttgt gctcagcgct
     3121 atttcaccct gtagcaaagg cacctcaagt cttccaccca cttcgggttt ccactgttta
     3181 gaacccaggt attcccatcc ttttggtttt ttatttccgg ctaaagtcca taaccagata
     3241 ttggagttat tcttaaaaaa atattttcca agtatgccat atacaccgtt agtcagtcgc
     3301 agcgggtcgc gcacatccat ctggtcgaac cacatgagag gcctgaacat tttagctgta
     3361 ccaaaattta ttttctgcaa accggcgcgt acttcccagt ggtcagtgga atacctcaac
     3421 cacacccggt aagggcttat ttcaccattt ttggaagaat aatcaaggct ggtgaagttt
     3481 agtgcaccat tgagatgaag tgatgcctcg aaatccagtt ttgaatgttt acggatatca
     3541 aaattaccaa gtaatttggg aacaaaacgt tcacccgctt gccagatcag tggatttgaa
     3601 aaacgggtaa cttcccagac ggtagcctga ccactgaagg taacagcatg cgtaacctca
     3661 gtggagtcat ttacttcctg agcctgcacg gcttcaagcc ctggaagcat catcagaagg
     3721 aaaaaagttt tcaaaaaagc cctcatgttt ttttctggct atatactttt acttacctga
     3781 ggtaaaggct gtaaagctac aaaagattgt taataaatca aataattgag gatgtttctt
     3841 tacagagaaa ccgacaaatt caaaaaaaaa caattcattt ttagatacta caaaaaaata
     3901 actacatttg cttattctat taacaaagaa caaaaaaata tccggcttat aacccggata
     3961 ttctcaaaaa aaataataaa tcgaattttt atttcctgta aataatatga aaaaggaata
     4021 tacattcaac gaacttctgc acaaagctgc atcttattgt tccatttccg aacattgcat
     4081 ttcagacgta aatgacaaac tgaaagcctg gcaggtagat gaggcagatc gtaagaaaat
     4141 tatcgaccag ttgtgtgagg atgattttat caacgaaaaa cgattttgca aagcttttgt
     4201 acgcgataaa ttccatctga ataaatgggg gaaaataaaa atctcgatgg ctttgcgcga
     4261 aaaagggctg agcaatgaac tcataactgc agcattgaaa gtgattgacg agggagaata
     4321 tgaggaattg ctggcttcat tgctgaagtc aaaacagaat acaattaaat acgagtttga
     4381 atacgaaaaa cagggaaagc tctatcaatt tgcccaaagc cgtggtttcg aaaatcaggt
     4441 gattgaacgg gtattaagac ttatttagac agatgcaaat gatataacat tacgttggtc
     4501 tgtctctgaa aaaatattga acttttaatg atcataaaaa aaacggctgg aaatctgaat
     4561 acagattttt agccgttatt tttttaagaa tgctgaaact ttgttcagga ttctaaaatt
     4621 tgcttcaaat gacttatttc gaagctgtga tagaaacttt gcggaattct ttcagcaatt
     4681 tttccagttc caatgaagct ttgcgggcac gtgcaccagc ggctttgttg tttttttcta
     4741 cttgcaaacc tgcgttttca gcgaaagcat caatttgttc ttgaattttg gctaccaatt
     4801 ctttcataag acttttgtat ttaagtttat taaaataaat ctatcgcaaa aatacaatct
     4861 tttatcttta ttccaacact ttttacaaaa aacacgttaa ttttttcgta aaaaatccat
     4921 tttttttggg gttgcaggtg tttttggtct tttttggagc tgaaacttcc gttgttagcg
     4981 gtttcgcaac agttttgaca cattattatc accacaagag aaagaagaca aaagacaaaa
     5041 tcagttacta gatacaagtt acaagttaca agaaaacata aatcagtaat cagtaaacga
     5101 aaaccagttt caagttaatt cgtctcaatt cgtgtaattc gttaaaaaac agttcaacat
     5161 tccacactac acgtatcaac tatctctcct ctcggccata agtaaaaaca attcatacac
     5221 cctggggttt ccgggtgtta cttccctgtg tagggtgttg taagttttag tcataagaac
     5281 ggtaaatccg gcctggtgga aaatgtcggc cagttggtcg gtatcaaacc cccggtgagg
     5341 cacggcttcg aagtgatgga aggagccatc ctcgctgcgc aaatcaccca ccaccacgcg
     5401 ggcaccgggt ttagtgatgg tgtgcaggtg ttgcagggtt gcggctatgt cagtaatgtg
     5461 atgaaaggcc atgttggaaa ataccagatc gatatcaggc tgagtgtaag cagtcacttc
     5521 gccgtgcaca atctccacat tgggtagctc gtcggttttt tgcttcagaa tggcgagcat
     5581 ggcttccgac gtatcttcaa aaacacagga tttgagcatc ggtaaaagct gcagcccaac
     5641 caatcccgtt ccggctccaa tttcgagggc tttccagtgg ggttgcagat caacgtgttt
     5701 cagcagttca gcggcaaatt tatcggacat tttgatcttg tcgggttgat cccatccggc
     5761 agctttgtgg gcaaatgtat ctgtcatatt attttagaat tactttttag atggtttttt
     5821 atcattatca caataaacta tattgaggag agaaaagttc aactaatatc cgcaaatttt
     5881 cacattaagc aaaatgaata aatatagtat atttgtaggt ttttagaacc aggactatta
     5941 gaaccaagag tcaagaaaat agaaccaaga atcaaatgtc ttctagtcca cttgtcaact
     6001 gcatcttact tgttttactt tataaacttt ttaacttgac aaactttaca aacttgataa
     6061 acttactaac ttgataaact atccaacaat gaaatcaact ttaaaaacaa ttcttatggc
     6121 aattgcactg acagccgctg tgtcggcttg tgatacgaag aaggaagatg ctccgcttat
     6181 cgaaaagcag gtaccggtca tcgaaaacgg gcggatgaca cccgaaattc tctggtcttt
     6241 tggtcgcgtg ggcaatgtgg aagtttcgcc cgacgaaacc aaaattcttt acagtgtgtc
     6301 gtattacagt gtggaacaga acaaaagcaa ctcggaacta ttcgtgatga atacggatgg
     6361 ttcagggaaa aagcaaatca ctaaaacagc caacaaggaa atcgcggcca aatggatgag
     6421 tgacagcaaa cacatcgcct ttctgagtaa cgaaagcggc agtatgcagc tgtggacgat
     6481 gaaagacgat ggtaccgccc gtacccgtct caccgaccgt gaaggggata tcagtgattt
     6541 cagcttctcg cccgatgaac gaaaagtgct gctggtagcc gatgtgaaat acggaaagcg
     6601 taccgcggat attcatcccg atttgcctaa ggccagtggt gttatcgtca acgatctgat
     6661 gtacaagcac tgggatgaat gggtgcagac tgttcctcat ccctttgtag cagatatcga
     6721 aggcggaaaa ttgacgaatg aaagagatat attgaaagat gaaccgtacg aaagtccgat
     6781 gaaaccgttt ggcggcatcg aacaactggc ctggagcccc gacggaaaaa caatagctta
     6841 tacctgccgg aagaaaacgg gaaaggaata cgcactttcc accaactcag acatctattt
     6901 ctatgatctg gaaaccggaa aatcggtcaa caaaaccgaa ggcatgatgg gctatgacca
     6961 gaaccccgtg ttttcgcccg atggcaaatg gctggcctgg gagagtatgg aacacgacgg
     7021 atacgaatcg gacaagagtc gccttttcgt aatggatgta aaaaccggtg agaagaaaga
     7081 tgtcagcgct gcttttgatc agaacagcaa cgggctgaaa tggtcggttg acggtcaatc
     7141 catctacttt atcagttgct atcacgcagt aacgcagata tacaaggctg acctgaaaga
     7201 tggtaaaata atgcctgtta caaagggtgt gcatgattac ggaacggtga taccaatgaa
     7261 aaatcaactg atcggggtta aacaatcgat gagcaaaccc gatgaaatat atgccatcaa
     7321 cgcttcaacc ggtgaagaaa aagaactctc gtttgagaat aaggatatac tcgacaaact
     7381 ggaaatggga aaagtggagg aacgctggat taccaccacc gacaataagc agatgcacac
     7441 ctgggtaatt tatccgcctc atttcgaccc ggcaaagaaa tatccgaccc tgctgttctg
     7501 tgaaggaggt ccgcaaagta cggtaagtca gttctggtcg taccgctgga attttcaaat
     7561 catggctgct aacgattata ttattgttgc ccccaatcgt cgcggtttac ccggatttgg
     7621 ttacgaatgg ctcaaacaaa tcagtggcga ttacggcgga caaaatatga aagattacct
     7681 ctctgccatc gatgcagtgg ctaaggaacc gtttgtggat aaagataaac tgggttgtgt
     7741 gggtgcaagt tacggtggtt tttcggtgta ctggcttgcc ggtcatcacc aaaaacgctt
     7801 caaagcattc attgctcacg atggcatgtt caacctgccg cagcaatacc tggaaacgga
     7861 agagaaatgg ttcgtcaact gggacctcgg tggtgcttac tgggaaaagc acaatgccat
     7921 agcccaacgt tcctatgcca actccccgca tctgtttgta gataaatggg atacgccgat
     7981 tttgattatt cacggtgaaa aagattaccg cattctggcc tcacagggta tggccgcttt
     8041 tgatgctgct gtgctgcggg gcgtacctgc cgaaatgctg atttatcccg atgaaaacca
     8101 ctgggtacta aaaccccaaa acggcatcct ctggcaacgt acatacttcg gctggctgga
     8161 taagtggctg aaatagggaa gccccccagc cccctaaagg gggagaggaa gagttattgg
     8221 ctattaagtt atttgttcca aaaataagtg cgggacattt attgagttat ttgttctgac
     8281 atttgttttc tgattactca ttaaattctt tgtacattat tgaacctccg accctgtccc
     8341 gaactagttt cgggcgggag cagggggttt gattatttat taattaatgt taaactacga
     8401 attagtctgc aacgttttga aggtattaat catcatataa taaacaggga atggatctga
     8461 tggacgaaca acgtttaatt gccggatgta aacgtggcga acagtgggca aggagggagg
     8521 tttatgagaa atatgcctct gcaatgttat ccctgtgcgt gcggtatgtg gccgattacg
     8581 aggctgcgcg cgacgtgttg caggatggat ttgtgaagtt gtttacacgg gctgaacagt
     8641 acgatggaac aggaaatttc ggtggatgga tccggcagat ttttgtaaat acagcgctgg
     8701 agtatctgag gagacaaaac agactggaaa cagatgcgat atcagaagac ttgaaagaaa
     8761 ggaacgataa ccggaatttc tcacccggta agctcactgc cgatgatctg atggaatgtg
     8821 tagcggaatt gccggagaaa ctcagaactg tttttaacct ctacgccatc gaaggattta
     8881 gtcacagcga aatagcagcg atgaccggaa ttcaggaaag tacgtcgaga tcgcagtttt
     8941 tacgggcccg acagctattg caacagaaag tatccctatt aatgaaaaat gaccatgcag
     9001 gaaaaaaata aacataccaa cgaaccagac gctttcagtc aatgggccag gcagcaactg
     9061 gaaaatcatg agttgccggt ggatgaaagt ctgtggcagg gcattgaaca gaaacttgct
     9121 cctgctccca aaagacgtat tcccgtgtgg tactggctgg caggtggtgt agctgccgtg
     9181 gtggctttga tgctgctgat acgtccgtac tatactactc agacagataa agaactggct
     9241 caaacaaccc tttcacgtga acctgtaact aaatccgcag cagtatcgtc cgaaaatccg
     9301 gcaagactat cgggcgtacc tgcggaggat gacatcatgc atccggcagc tcaggcagat
     9361 gcaagcggct ctgccgggct gttaaattcg cagaaagtcc aacagacaaa tcaatcaaca
     9421 ccggtgaaaa ctgctcatgc agtcagcaat caaaagaaga ccttcatagc ttctgcttca
     9481 actttttcaa cgaaatcgac ttcagcttca actgcaatta acaccataaa tgataataaa
     9541 cggactgtaa ccgacgaaaa gcaaatagct gctgttgctg atgaaacagc aaaaaaatcc
     9601 gacattaatc cagcaaaaac cgaaaagcag caaatcactt cgttgcccga cctgtcggat
     9661 tatcccgaag cacccgccga tgaacccgca aaaaataaga agaaacaaca cctgctgctg
     9721 gctgctaatg tgggagccgg aagcacactg atttctaacg gaagtggatt atttcttgag
     9781 tcgggagcga tgagcgatgc tgcactggtc aacagcacca gtgtaagagc cgaatatgca
     9841 gccgatataa aagaagcgga ttatttcaat gaagttcagc atctgccacc cgtttcattc
     9901 gggctgatgg ctgaattgcc gctgagtaaa agctggagtg tggagagcgg actggtatac
     9961 acgtatctgg aaagccggtt tacccgcact gccaatccca ctggtaccgg tcatctcaaa
    10021 ctgcattacc tgggacttcc cctgtatctg aaagcaaaac tatggcacga ccctacctgg
    10081 agcatttatc tcaatgccgg accaatgatc gaaaaaggct tgcaatccgt ctattcccag
    10141 gatattgatt acacctggtc catcacccat accgatgtac attcgcgcat cgaagggctg
    10201 cagtggtcgc tcaataccgg attgggagtg aattataaaa tacagaaagc cctgagtgtg
    10261 ttttttgaac caaaactgac atactacctg gataataacc agcccatgag tgcccgaacg
    10321 gagcagccgc tggtattcgg ggtaaacgga ggagtgcgga ttgagttata aaactaattt
    10381 gaaaatgtgt caatttgaaa atttgaaaat gggatgattt gaagatgagg taatttgaaa
    10441 atgaggcaat ttgaaaattt gaagatgggg taatttgaag ataagataat ttgaagatgg
    10501 ggtaatttga taatttgaaa ataggatgat ttgaagattt gaagatgggg taatttcaga
    10561 aaaacataac aatctatata aaa
//
LOCUS       DAOPZK010000066        10469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_7370_length_10469_cov_20.627233, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000066 DAOPZK010000000
VERSION     DAOPZK010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10469
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7370_length_10469_cov_20.627233"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..763
                     /locus_tag="PKH68_05445"
     CDS             194..763
                     /locus_tag="PKH68_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNX12415.1"
                     /translation="MEIIQSLEPLLRAFWYIAIPVSIIFIIQAIMTFVGGNSMDGVAA
                     DFDSDFSDTDAPFQLFSLRNLINFLLGFSWAGIAFYNIIGSKTLLFILAIVVGIVFVG
                     IFFVVIKQLMRLAEDDSFKPDETLGKTAEVYLTIPKNRTGNGKILISVKGSVRELEAM
                     TDSPEPIPSHTTVKVVRIENKNVLIVDKI"
     gene            768..>2208
                     /locus_tag="PKH68_05450"
     CDS             768..>2208
                     /locus_tag="PKH68_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNX12416.1"
                     /translation="MLSSPLSLIVVLAIVIFVTILALVSRFKRCPSDKILVVYGRTGG
                     SSAKCIHGGGAFIWPVIQDYAYLDLKPLSIEANLTNALSRQNIRVDVPSRFTIAISTE
                     PETMNTAAERLLGLIPDKIKELASDILFGQLRLVIATMTIEEINSDRDKFLEAISKVV
                     DNELRKVGLKLINVNVTDIKDESGYIEALGKEAAAKAINEAKISVAEQEKIGETGKAV
                     ADKDREINIATAMKDEMIGKAEADKLTRIKTSEANATAVQGENTAKITIAQSDALRRE
                     KEAESARLAIAAEKVQEAKALEEAYVAEQMAENARAERERSTETANIVVPAQIAKQKL
                     VIEAEAEAERIRLQAKGEADAIFAKMDAEARGLFAILTNQARGYADVVKAAGGDADKA
                     YQLLMIEKLPDLVKTQVEAVKNLKIDKVTVWDSGNGSDGKNSTANFMSGLLKTIPPLD
                     DLFKSAGMNLPSFLKSAENAEYSPVTEKTE"
     assembly_gap    2209..2308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2598..3659
                     /locus_tag="PKH68_05455"
     CDS             2598..3659
                     /locus_tag="PKH68_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX12417.1"
                     /translation="MNNLRTGIIGFGFSGRVFQAPFIDAVEGFELTKISTANPDSIKL
                     IENRYPDTIIVPRAEEIINDTSLDLVIVTSPNIFHYQWTKEALLAGKHVVVEKPFTNT
                     AAEADELIELSKQTGKTLTVYHNRRFVSDIKTARKLLFSGLLGDVVDYEAHFDRFRPE
                     AQPAGYWREDPLPGSGILYDLGSHLIDQALYLFGLPLAVTANICRQRPWAKADDCFDV
                     MLHYPTHTATLKSSMLAKIPGPTIQIHGTKGTFVKYGLDVQEDNINKGLIPNTPDWGK
                     EPEMGWGKLVTEFNGVKLKALVESENGDYREFFINLRDSIQGKVDLAVKPEDARTVIR
                     IIELAFQSSNERRTVDFSK"
     assembly_gap    3801..3900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3916..4782)
                     /locus_tag="PKH68_05460"
     CDS             complement(3916..4782)
                     /locus_tag="PKH68_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNX12418.1"
                     /translation="MKWINRNDDESNYEDRRGMSTGRKMAAGGGIGTIIILALYLFTG
                     QDFSPVVDMFSGGGATQTETVDPTRAKENEDLKVFSLGVFNSANQVWTKIFNEQMGAE
                     YRKPTFVTFSDQTMSECGGASGATGPFYCPGDEKVYIDLSFFHQLATRFNAPGDLAMA
                     YVTAHEVGHHVQKLLGLTDKMQAYRGRISEEEYNQLSVRLELQADFYAGLWARYAEAM
                     NIIKLEEGDIESALGAANAVGDDTIQKETQGYTVPDSFTHGTSAQRMRWFMKGYQTGD
                     YRQGDTFNAATL"
     gene            complement(5002..5664)
                     /gene="ung"
                     /locus_tag="PKH68_05465"
     CDS             complement(5002..5664)
                     /gene="ung"
                     /locus_tag="PKH68_05465"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNX12419.1"
                     /translation="MDVRIEESWKQRLSPEFEKPYFKSLTDFVRNEYSTKQIFPPASL
                     IFNAFNCCPFDKVEVVIIGQDPYHEPGQAHGLCFSVSQGIDIPPSLVNIFKEIERGLG
                     KPVPASGDLTRWAQQGVLLLNATLTVQNHRAGSHQGKGWETFTDAAIRVLAAEKENLV
                     FMLWGNYAQQKGAFIDSTKHLVLKSVHPSPLSAYRGFIGNNHFALANNYLKEHGKKEI
                     EW"
     gene            complement(5692..8097)
                     /locus_tag="PKH68_05470"
     CDS             complement(5692..8097)
                     /locus_tag="PKH68_05470"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNX12420.1"
                     /translation="MKIKIGIFLAFLLVMSATAQTKKSVEAYRIATPLTIDAKLTEPD
                     YARALPAKDFVQLQPYNGKPSFQPTEAYFFYDETAIYVGVKLYDNPDSIFNYLTERDN
                     PGMADYFGVYIDPYNQGQLAFGFFVTPAGVQADIKATKSDEDTEDTNWNAVWESKTQI
                     NSDGWTVEMKIPYSAIRFTKNQKSAWGLNMFRNIRRHNSNNSWNFIDRTVSGFIHQEG
                     QLTGIENIKPPVRLSFSPYLASYYQPKSKDAKAGFLYKGGMDLKYGISDAFTLDMMLI
                     PDFGQIQSDDQKLNLTPYELYYDEKRQFFTEGTELFHRGGIFYSRRIGAAPKFNASAL
                     LKENETIDYNPGETQLVNATKISGRTEKGWGIGVLNAMSLASEAVLKNTETGTTREVQ
                     VQPFTNYNVAVVDKSLKNNSYISLINTNVSMAGNPFHANVTATEFQLRDNTKTYALKG
                     KAGISVRGNEKSETGYYSTLRFEKNKGTWLYGAAENLYSDKFNPNDLGYLERNNMSNT
                     EAWFYYQAIDPKGIYLQWNGGLWMNYNRMYNPNTLFDTEGGYEAFMQFKNNYYFHTNG
                     SVSSRQYNYYEPRVKGRYYLKPVRYSANFELSTDARKPLSFDAGYGFSTQPAIGTVEN
                     AIEIGANLRLGQQFVFNYEIEGHKSKNERGYVMNNSAGDTITFARRDVTSAVNTLSAS
                     YIFNYKTSLTLRMRHYWSGVGNKEFYLLQPDGSLSSYPDFTQNKDMNYNAFTIDMVFR
                     WIFAPGSELSLAWKTNSYAGEDRVVYNYFDNLNNSWLNQKNSLSLKVLYYIDYNKLRK
                     KKI"
     gene            complement(8122..9303)
                     /locus_tag="PKH68_05475"
     CDS             complement(8122..9303)
                     /locus_tag="PKH68_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNX12421.1"
                     /translation="MKYNFDELIDRRNTGAVKTDLCKKMFGTDDLIPLWVADMDFRTP
                     DFIIDAINNRCKHPILGYTVPDEEYFNSIIRWIDARHGWKLERNWLGFLPGIVPGLAF
                     AVNALTQTGDQIIIQPPVYPPFIQVPSKNGRELIYNPLKVVEGRFEMDLKDLEYKITD
                     RTRMFILCNPHNPGGRTWDADTLIKVAEICHKHGVLVVSDEIHSDMALPGNVHTPFAS
                     VSELAEQNSITYMAPSKTFNMAGLVSSSYIIPNSEIRKKFSDFMDNSELANGNIFAYV
                     AAQAAYEKGTEWLSEMISYIQGNVDYIVEFLEKNIPQIKPMIPQASFLIWLDCAGLEM
                     NSHQLQEFMVKEARLGLNKGTTFGPGGEQHLRLNIGCSREVLKQAMEQLKSAINSRLN
                     K"
     gene            complement(9468..>10469)
                     /locus_tag="PKH68_05480"
     CDS             complement(9468..>10469)
                     /locus_tag="PKH68_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12422.1"
                     /translation="QMNVNGGFRSISSADWTPYSSISSYLNPTGNGSKSAPFRYNPQD
                     KGKNSTQSILRNTPAANSLVAELAQTIAVKEMLGEDKYTDALMLQFTVKTPNQTTSSL
                     TTAEQEDMYLRLDRDIQNLMSSLTSRVGSDKVLFFLTGGTEDLHSPVELGNNRIPAGY
                     FNANRSLSLVNSYLMAIYGQEKWISGYYGKNIFLNKKRMEEKKMNIPEVLERTAEFLM
                     EFEGIQAAYPINEIINYAGSSIDVRSKYRNSYHKNTSGDIVITLLPGWVEVDDKNQLV
                     GTANAPRVYIPFYLLGAKIKPQKVNVACQTIDIAPTLARLLGIPTPNACVGKSLLDD"
BASE COUNT         2843 a         2183 c         2223 g         3020 t
ORIGIN      
        1 taagtatatt taatttaatg acagagaatt aatttgtcaa aaaagggttg taaatcagtc
       61 aataataaga gtaaaaaatt ccacaactga ggtttgaatg ttttacgtta aaaatatatt
      121 taaaaaatta tattgtgatt tattcttgaa tattcataag atatatttaa ctttggagtt
      181 aaaattttac accatggaaa taattcaatc tttagaacct ttacttcgtg cattttggta
      241 cattgccata cctgtaagca tcattttcat cattcaggcc attatgactt ttgtcggagg
      301 caattcaatg gatggtgtag ctgccgactt tgacagcgat ttcagcgata cggatgcacc
      361 ttttcagcta ttttcgctcc ggaatcttat caattttctt ctgggattca gctgggcagg
      421 tattgctttt tacaacataa ttggcagtaa aacgcttctt ttcattctgg caattgtcgt
      481 tggaattgta tttgtgggga tattttttgt ggtaatcaaa caacttatgc gtttggctga
      541 agatgattca tttaagccgg acgaaacact tggcaaaact gccgaagttt atctcactat
      601 tccaaaaaac cgaacaggaa acggaaaaat tctgattagc gttaagggtt cggtgcgtga
      661 gctggaagcc atgaccgact cgcctgagcc cataccatcg cacacaacgg tgaaagtggt
      721 tagaattgaa aataaaaatg tattaatcgt agataaaatt taaaattatg ctttcatcac
      781 ccttgtcgct tattgtagta ctggcaatcg ttatttttgt aactatcctt gcccttgtat
      841 cccgtttcaa acgttgtcca tcggacaaaa tcctggttgt gtacggtcgc accggcggtt
      901 catcagccaa atgtatccac ggcggtggcg cctttatctg gccggtaatt caggattatg
      961 cctatctgga tctgaaaccg ctctctatcg aagccaatct gaccaacgca ttgagccgtc
     1021 aaaacatccg cgtagacgtg ccttcacgct ttaccattgc catttctaca gaacccgaaa
     1081 ccatgaatac ggcagccgaa agattgcttg ggcttattcc cgataaaatc aaagaacttg
     1141 cctccgatat tctgttcggg cagttgcgtc tggtaattgc caccatgacc atcgaagaaa
     1201 tcaactctga ccgcgacaaa tttctggaag cgatatccaa agttgttgat aatgagctcc
     1261 gaaaagtggg tctgaaactg ataaacgtaa acgtgaccga catcaaggac gaatccggct
     1321 acatcgaagc attgggtaaa gaagctgccg caaaggccat taacgaagcc aaaatatcgg
     1381 tggccgaaca ggaaaagata ggtgaaaccg gtaaagctgt ggctgacaaa gaccgcgaga
     1441 tcaacattgc aactgccatg aaagacgaaa tgattggtaa ggcagaagca gataaactta
     1501 cccgtatcaa gacttccgaa gccaatgcaa ccgctgttca gggagaaaat accgctaaaa
     1561 taacgatagc gcaatcggac gctctgcgcc gtgagaaaga ggccgaatcg gccagactgg
     1621 ccattgctgc cgaaaaagta caggaagcca aggcgctgga agaagcgtat gtagctgaac
     1681 aaatggctga aaatgcacgt gcagaacgcg aacgttccac cgaaacagcc aacatcgtgg
     1741 ttcctgctca gattgccaaa caaaaactgg taatagaagc agaagccgaa gccgaacgaa
     1801 ttcgcttaca agccaaggga gaagccgatg ctatttttgc gaaaatggat gccgaggcac
     1861 gcggattatt tgccattctg accaatcagg cacgaggtta tgccgatgtg gtaaaagccg
     1921 ccggaggtga tgcggacaaa gcttatcagt tattaatgat agagaaatta cctgatttgg
     1981 tgaaaacgca agtggaagca gttaaaaacc tcaaaattga taaagttacc gtttgggatt
     2041 cgggaaatgg cagtgatgga aaaaattcaa ctgcgaattt catgtccggc ttgctgaaaa
     2101 ccattccacc ccttgacgac ctcttcaaat cggccggaat gaaccttccc tccttcctta
     2161 aaagtgctga aaacgctgaa tactctccgg taacagaaaa aacagaagnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa aaggaaatga agcaaagaaa taatatattt
     2341 ttgcagtaga gaaaaaacta aatcagaatt tcagagacaa attcattttt gtctctgttt
     2401 tcatttttgt aaaaagaaag aggctttgtt agtcataatt tgaaatttca attgataaaa
     2461 aatgtttaaa gaaacagaca atgcgagttt tccaatcttt ttatttttct gaaaatcatt
     2521 tttaattatt caatgatgcg ttattttatt taactttgaa tttgatagtt tcaccgatta
     2581 aaaaaaacaa ctttaatatg aataatttac gcaccggcat tattggattt ggattttcag
     2641 gaagggtatt ccaagcccct tttatcgacg cagtggaagg atttgagtta acaaaaatca
     2701 gtactgccaa tcctgattcg attaaactta ttgaaaaccg ttatcctgac acaataatcg
     2761 ttccccgtgc agaagagatt atcaatgaca ccagccttga tctggtaata gtcacttcgc
     2821 ccaacatctt tcattatcag tggacaaaag aagcattgct tgccggaaag cacgtagtgg
     2881 tcgaaaaacc atttaccaac acagctgcag aagccgacga actgattgaa ttaagcaaac
     2941 aaacgggtaa aacactaact gtataccaca atcgccggtt tgtaagtgat ataaagactg
     3001 cgagaaaact cttgtttagt ggattattgg gtgatgtggt ggattatgaa gcccatttcg
     3061 atcgtttccg tcccgaagca cagccagcag gttactggcg cgaagatccg ttgcccggtt
     3121 cgggaattct ttatgattta ggttcgcatc ttatcgatca ggcattatat ttatttgggt
     3181 tacccctcgc cgttactgcc aatatctgca ggcaacgccc ctgggctaaa gccgatgatt
     3241 gttttgatgt gatgctacac taccccactc ataccgcaac attgaagtcg tccatgcttg
     3301 caaaaattcc cggacccact atccaaattc acggcacaaa aggaacattt gtgaaatacg
     3361 ggctggatgt tcaggaagat aatataaaca aagggctaat tcccaacaca cccgactggg
     3421 gtaaggagcc ggaaatggga tggggaaaac tggttaccga atttaacgga gtaaaactga
     3481 aagcacttgt ggaaagtgaa aatggagatt accgcgagtt tttcatcaac ttacgcgatt
     3541 caattcaggg gaaagttgat ctggcagtaa aacccgaaga tgcccgaact gtgatccgga
     3601 ttattgaact ggcattccaa agcagcaacg aaagaaggac tgtggatttt agtaaatgat
     3661 gttggttatt gggttattgg gttattgggt tattggttat tggttattgg ttattgagtt
     3721 attgagttat tgagttattg atattttctt taagcccctt taggggtttg gggcagtctt
     3781 tgttcttttg tcttttgtct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 tcttttgtct tttgtctata atgtcgccgc attgaaggta tcgccctgtc ggtaatcacc
     3961 tgtctgatag cctttcataa accacctcat acgctgagcc gatgtaccgt gagtgaacga
     4021 atcgggtaca gtatagccct gagtctcttt ctgtatggtg tcgtcaccta ccgcattggc
     4081 agctccgaga gccgactcga tatcgccttc ttccagtttg ataatattca tggcttcagc
     4141 atatcgtgcc caaagtccgg cataaaaatc agcctgaagt tcgagtcgta ccgaaagctg
     4201 attgtattcc tcctccgaaa tccttccgcg gtaagcctgc attttatcgg tcagtccaag
     4261 tagtttctgt acgtgatgtc ccacctcgtg agcagttaca tacgccattg ccaggtcacc
     4321 gggtgcattg aaccgggtcg caagctggtg aaaaaatgat aaatctatat aaaccttctc
     4381 gtcgccaggg cagtaaaatg gtccggtagc accgcttgct ccgccgcatt ccgacatggt
     4441 ctgatcagag aaagtcacaa acgttggttt gcgatactca gcacccattt gctcgttgaa
     4501 tatcttggtc cacacctggt tggcactgtt aaatacaccc agtgaaaaaa ctttcagatc
     4561 ttcattttct ttagcccgcg tggggtcaac agtttcggtt tgtgtggctc cacctccgga
     4621 aaacatatcc acaacgggtg aaaaatcctg tcctgtaaac aaataaagag ctaaaataat
     4681 aatagtacca attccgcctc ccgcagccat ttttcgacca gtagacatac cccgtcggtc
     4741 ttcatagttt gactcgtcgt catttcggtt aatccatttc ataatcgttt caattaaaat
     4801 attttgcgaa agatagtact ttttttattt ctgtcaaaaa tttattggtt attgagttct
     4861 tgagttaatt gttattgagt taataagtta ttagttattg agttattgag ccacttaccc
     4921 cccataaaaa ttgggttcaa agaaaaacag ttattgatta ttttctttaa gcccctttag
     4981 gggtttgggg cagtaatgac attaccactc aatttccttc ttcccgtgtt ctttcaggta
     5041 attgttggcc aaagcaaaat ggttgttacc gataaaacca cgatacgccg acaaagggga
     5101 tggatgtact gatttcagca ccaaatgttt ggtagaatca ataaatgctc ctttttgttg
     5161 tgcgtaatta ccccagagca taaaaaccag attttccttt tcggcagcca gcacacggat
     5221 agccgcatcg gtaaaggttt cccaaccctt accctgatgc gaacctgcgc ggtgattttg
     5281 tacggttagt gtggcgttga gcagcagtac tccctgctgt gcccagcggg taagatcgcc
     5341 acttgctggc accggtttgc ccagaccacg ttctatttct ttgaaaatat tgaccagcga
     5401 tggtggaata tctatcccct ggctgacaga gaaacagaga ccgtgtgcct gacccggttc
     5461 atgatatggg tcctgcccga taatcaccac ttccacctta tcgaacggac aacagttaaa
     5521 cgcattgaaa atgagggatg caggcggaaa tatctgtttg gtgctatatt catttcgtac
     5581 aaaatcggtc agtgatttaa aatacggttt ttcaaattca ggtgaaagtc gttgcttcca
     5641 gctttcttca attcgtacgt ccactattta ttggttttaa attttgtgag attaaatttt
     5701 ctttttcctg agtttattat aatcaatata atacagcact ttgagcgaca aactgttctt
     5761 ctgattgagc caggagttgt tcagattgtc aaaataatta tataccaccc gatcttcgcc
     5821 ggcgtaagaa ttcgtcttcc aggcgagcga cagttcggag cccggagcaa aaatccaccg
     5881 gaacaccata tcgatggtga atgcgttgta attcatatct ttattttgtg taaagtcggg
     5941 atagctggaa agcgaaccat caggctgcaa gaggtaaaat tctttattac ccactcccga
     6001 ccaataatgg cgcatcctca gcgtgaggct ggttttgtaa ttaaaaatat aggaagcact
     6061 cagtgtgttg accgccgatg ttacatcgcg ccgggcaaaa gtgatggtat cacctgccga
     6121 attattcatt acataaccgc gttcattctt tgatttgtgc ccttcaattt cgtaattaaa
     6181 cacaaattgt tgccccaatc gcagattagc accaatttct attgcatttt ccacagtccc
     6241 aatagccggc tgagtgctaa atccataccc ggcatcgaaa ctaagtggct tgcgggcatc
     6301 cgttgaaagt tcaaaattgg cggaataacg caccggtttc agataatacc ggcctttaac
     6361 acgtggttcg tagtaattgt actggcggga cgacacactg ccgttggtat gaaagtaata
     6421 attgttctta aactgcatga aagcctcgta tccgccttcg gtatcgaata gtgtgttcgg
     6481 gttatacatc cggttgtaat tcatccacag accgccgttc cattgcaggt atattccttt
     6541 tggatcaatg gcctgataat aaaaccatgc ttcggtgttc gacatattat tgcgttcgag
     6601 gtatcccaga tcgttgggat tgaacttgtc ggaatataaa ttctcggctg caccgtagag
     6661 ccatgtccct ttattttttt cgaacctcag ggtgctgtaa taacctgttt cacttttttc
     6721 atttccacgt acacttattc ctgcctttcc tttcaatgca taggttttag tattgtccct
     6781 gagctgaaat tcggtagcgg taacattggc atgaaacgga ttaccggcca tagacacatt
     6841 ggtattgata agactgatat aggaattgtt tttcagtgat ttatcaacaa cagccacgtt
     6901 gtagttggta aatggctgta cctgaacttc acgcgtagtg ccggtttccg tattcttcaa
     6961 aacagcttcc gatgcgagcg acattgcatt caatactcct atcccccatc ccttttctgt
     7021 tcggccggat attttcgtgg cgttgaccag ttgagtttca cccggattgt agtctattgt
     7081 ctcattttct tttagaagtg ccgatgcatt gaattttggt gcagcgccta ttctgcggga
     7141 gtaaaaaatc cctccacggt gaaacaattc tgttccttcg gtgaaaaatt gtcttttttc
     7201 atcgtaataa agttcgtaag gtgtaaggtt gagtttctga tcgtccgatt ggatctgccc
     7261 gaaatcgggt atgagcatca tatcgagagt aaatgcatcg ctgatgccat atttcaggtc
     7321 catgccgcct ttgtacagaa acccggcttt tgcatctttc gatttgggtt gatagtaaga
     7381 agccagatag gggctgaacg acaatcgtac cggtggtttt atgttttcaa taccggtgag
     7441 ttggccttcc tgatgaataa atcccgaaac ggttcggtca atgaaattcc atgaattatt
     7501 ggaattgtgc cgtctgatat tgcgaaacat attcagtccc caggcgctct tttgattttt
     7561 ggtaaaccgg atggcggagt acgggatttt catttctact gtccacccat cgctgtttat
     7621 ctgtgttttg ctttcccata cggcattcca attggtgtcc tctgtatctt cgtcgctttt
     7681 tgtagcttta atatcggcct gaacaccggc cggcgttaca aaaaaaccga aagccagttg
     7741 cccctgattg tatggatcga tgtaaacacc gaaataatcg gccattccgg gattatccct
     7801 ttctgtcagg taattgaaaa tactgtcggg attgtcgtac agcttcacac ccacataaat
     7861 ggctgtctcg tcgtaaaaaa aataagcttc agttggctga aaagagggtt ttccgttgta
     7921 gggctgcaac tgaacaaaat ctttggcagg cagtgcgcgg gcataatcgg gttcagtcag
     7981 ttttgcgtcg atagtaagcg gcgtggcaat ccggtatgct tcaacactct ttttggtttg
     8041 tgcggtggca ctcattacaa gcaaaaaagc caaaaatata ccaattttta ttttcataaa
     8101 aataactccg aaaaaaatta gctatttgtt caatcggctg ttaattgcac tcttcagttg
     8161 ctccatggcc tgtttcagca cttcccgcga gcacccgata ttcagtctca gatgctgttc
     8221 accacccggt ccgaaggtag ttcctttatt caatcccaat cgggcttctt tcaccataaa
     8281 ttcctgcagt tgatgagagt tcatctctaa tcccgcgcag tcgagccaaa tcagaaagga
     8341 tgcctgtgga atcatcggtt ttatttgcgg aatatttttt tccagaaatt caacaatata
     8401 atccacattt ccctgaatat aactgatcat ttcagaaagc cattcggttc ctttttcgta
     8461 ggcggcctga gcggcaacat aggcaaaaat attcccatta gcaagttccg aattatccat
     8521 aaaatcactg aatttctttc gtatttcgct gttgggaatg atataagacg agctcaccaa
     8581 cccggccata ttaaaggttt tactcggtgc catgtaggta atgctgtttt gctcagcgag
     8641 ttccgataca gatgcaaagg gggtatgcac atttccgggc agcgccatgt ccgaatgtat
     8701 ttcatcggac accacgagta ctccgtgttt atgacaaatt tcggctacct taatcaatgt
     8761 atcggcatcc caggtacgtc caccgggatt gtgcggattg cacaaaataa acatgcgggt
     8821 tctgtcggtg attttgtatt ctaaatcctt cagatccatt tcaaaccggc cttcaaccac
     8881 tttcaacggg ttataaatca attcccggcc atttttggaa ggcacctgaa taaaaggcgg
     8941 ataaaccgga ggctgaatga tgatttggtc gcctgtctgt gttagtgcat tcaccgcaaa
     9001 agccagtccg ggaactattc cgggtaaaaa acccagccag ttgcgttcca gtttccatcc
     9061 atgacgtgca tcaatccatc ggattatgct gttgaagtat tcttcatcag gaaccgtata
     9121 tcctaaaatg ggatgtttac acctgttgtt aatggcatca ataataaaat cgggtgtgcg
     9181 gaaatccata tcggctaccc atagtggaat caagtcatcg gtcccgaaca tttttttgca
     9241 caagtcagtt tttacggcac cggtattgcg gcggtctatt aattcatcaa aattgtattt
     9301 catgagttta tttttgttac tactaagcat tcgtacgcag gtggaaaaat tccttgttaa
     9361 tatgacgagg aagcgggtat tgaaaagtca tattttttaa ttatttaact attggaaaat
     9421 atttaaaaca ttttcacaaa aacaatcatt ctgttaccag gagatgatta atcatccaac
     9481 agtgattttc ccacacaggc attcggagtg ggaattccca gcaatcgtgc cagtgttgga
     9541 gctatatcta tggtttgaca ggccacattg actttttggg gtttgatttt ggctcccaac
     9601 aggtaaaaag gaatgtacac acgtggtgca ttggcggttc ccaccagttg atttttatca
     9661 tccacttcca cccaaccggg caaaagggtg attacgatat caccggaggt atttttgtgg
     9721 tacgaattgc ggtatttgga gcgaacatcg atagaacttc cggcatagtt gatgatttcg
     9781 ttgatgggat aagctgcctg aattccttca aactccatca gaaattcggc agtacgttct
     9841 aaaacctccg gaatattcat ttttttctct tccattcttt ttttattcag aaaaatattt
     9901 tttccgtaat atccgctgat ccatttttcc tggccataaa tagccatgag gtagctgttc
     9961 accagcgaca gggagcggtt ggcgttgaag tatccggcag gaatacggtt gttgccgagt
    10021 tccacgggtg aatgtaaatc ttcggtgcct ccggtgagaa aaaataaaac tttgtcgctc
    10081 cccacgcgtg aagtgaggga agacatcagg ttttgaatat cgcggtccaa ccgcaggtac
    10141 atatcctcct gctcggccgt ggtaagcgat gaagtggttt gattgggtgt tttcaccgta
    10201 aactggagca tcagtgcatc ggtatattta tcctctccga gcatttcctt tacggcgatt
    10261 gtctgagcca gttcggccac caggctattg gccgcaggag tgttgcggag gatagattgg
    10321 gtggaattct tacctttatc ctgcggattg tagcgaaatg gagccgattt cgaaccgtta
    10381 ccggttggat tgagatagga agaaatggaa ctgtaaggtg tccagtcggc cgaactgatg
    10441 gatctgaaac caccgttcac attcatctg
//
LOCUS       DAOPZK010000067        10464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_7375_length_10464_cov_22.674512, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000067 DAOPZK010000000
VERSION     DAOPZK010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7375_length_10464_cov_22.674512"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKH68_05485"
     CDS             complement(<1..359)
                     /locus_tag="PKH68_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNX12423.1"
                     /translation="MPKIQFLDPKKSRQPGFIEFQPIPVNQYQKKVKDEKENFTDDEF
                     KAIYHDMALIREFETMLNLIKIKGEYNGTPYNHPGPAHLSIGQESAAVGMAWTLTVDD
                     FIFGSHRSHGEILAKGMS"
     gene            complement(527..1882)
                     /gene="lpdA"
                     /locus_tag="PKH68_05490"
     CDS             complement(527..1882)
                     /gene="lpdA"
                     /locus_tag="PKH68_05490"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNX12424.1"
                     /translation="MIDLLIIGGGPAGYVAAERAGHHGLQVVLFEKKSMGGVCLNEGC
                     IPTKTLLYSAKTYENALHGDKYGVYGENIRFDYGKILARKNKIVRKLVAGVKSKMKEN
                     NVTVIDGEAMIQGRSEKGVEVTCNGESYLVKNLLICTGSEASVPPIPGLAEAGDTILT
                     NREILDLKELPNSLVVIGGGVIGMEFASFYNSLGVKVTIVEMLPEILGGLDFEISAML
                     RDIYTKKGIEFNLNAKVVQIDGNKVVFEKEGKTETIEGDKILISVGRRAVTKGFGLEN
                     LNVEMTRTGIKVDEKMRTNVPGVYAAGDVTGFSLLAHTASREGEVVVNNLTGRIDTMR
                     YNAIPGVVYTNPEVAGVGETEETAKAKGIAYKVAKLPMTFAGRFVAENEGGNGLCKIL
                     VGEKHGEVIGVHMLGNPCSEIIYGACMAIEQEMTLEAMQEVVFPHPTVSEIFKETIFS
                     V"
     gene            complement(1981..3333)
                     /locus_tag="PKH68_05495"
     CDS             complement(1981..3333)
                     /locus_tag="PKH68_05495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNX12425.1"
                     /translation="MAVVVVMPKQGQSVESCIITEILKKKGDAVKKGDILFTYETDKA
                     SFEEESPADGVVLECLYNDGDEVLVLENMMVIGQPGEEYVALIGGNAPAAPKAEMPET
                     FAANNITQSGSSITETVTIRSVEGLEFGSNISPRARILASREAVNITQIEGTGPKGRI
                     VERDIEKVLENQPKLTPLARKIATESGLQPQGTGSGLAGTAKSTDLLSSLTNETYGLD
                     YNDKKISNMRKIIAKSMHASLQNSAQLTHHLGADARRILELRKQVKSAMEAGKIATNI
                     TLNDMVCFAVIKALKKFPNVNSHFLGDTMRLFNKIHLALAVDTDRGLMVPVVRNADDL
                     SISGLANQFKEIANACRKGSINPELLSAEAATFTVSNLGNYGVEMFTPVINLPQTAIL
                     GVNTIVPRPKDLGDGVYAFVPYIGLSLTYDHRALDGGEATRFVKQIAVEIENLELDVF
                     "
     gene            complement(4001..>5377)
                     /locus_tag="PKH68_05500"
     CDS             complement(4001..>5377)
                     /locus_tag="PKH68_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX12426.1"
                     /translation="MSRMTGDVVEVEASIISSLDMVLKNPVMILIYVAVLFAMSWELT
                     LFVMVLLPISAWLIGVIGRSLKKRSTIGQEQTGELLSQIEETLGGLRIVKAFNAEEKL
                     TVRFSHLNEKIRHTFNRIHRKYNLAHPVSELFGTIVIAILIWFGGSLILEQNSSIGAA
                     QFIYYLVIFYSIVPPAKELSKASYSIQRGLASLHRIDKILMQENNIPDPATPRELPHI
                     VEKIEFKNLSFKYQSDWVLQNINLTIPLGKTVAIVGQSGSGKSTLVDLLPRFYDPAEG
                     GVFFNGVNIKDFRKSDIRSLMGNVNQEAILFNDTFYNNIAFGVSQASPEQVENAARIA
                     NAHDFIMATENGYESTIGDRGSKLSGGQRQRISIARAILKNPPILILDEATSALDTES
                     EYMVQEALDNLMKNRTTVVVAHRLSTIKRADLICVLHEGKIVEQGKHEELMALGGYYK
                     RLVDMQSF"
     assembly_gap    5378..5477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5478..5677)
                     /locus_tag="PKH68_05505"
     CDS             complement(<5478..5677)
                     /locus_tag="PKH68_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX12427.1"
                     /translation="MFDFLTLIKRFAPPYKKYIFLSLAFNLISTVFSLFSFAAIIPVL
                     QILFGLTKSDTVYIPWNWNEGLT"
     gene            complement(5717..7060)
                     /gene="purB"
                     /locus_tag="PKH68_05510"
     CDS             complement(5717..7060)
                     /gene="purB"
                     /locus_tag="PKH68_05510"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNX12428.1"
                     /translation="MSITSLTAISPVDGRYSSKTEPYSAYFSEFALIRFRVLTEVEYF
                     IALCELPLKQLESVPSNILPKLREIYGQFSLEDAEKIKEIEKVTNHDVKAVEYFLKEK
                     FDEIGLGSFKEFIHFGLTSQDINNTSVPLSIKKAMEEVYYPELDSLIKKLTRMADEWR
                     DVSMLAKTHGQPASPTRLGKEIMVFVSRLNQQLTLLRMVPHSAKFGGATGNFNAHHVA
                     YPAIDWRSFANKFTHEKLGLEREQWTTQISNYDNLAALFDALARINTILTDFCRDIWS
                     YVSMEYFKQKIKAGEVGSSAMPHKVNPIDFENAEGNLGMANAIFTFLAQKLPVSRFQR
                     DLTDSTVLRNVGVPFSHTLIATQSILKGLDKLLLNETALYRDLDNTWAVVAEGIQTIL
                     RREEYPAPYEALKALTRTNEAINEKSFREFIDQLDVSAAVKEELRRITPRNYTGI"
     gene            7244..7915
                     /locus_tag="PKH68_05515"
     CDS             7244..7915
                     /locus_tag="PKH68_05515"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNX12429.1"
                     /translation="MKYIKIHKEGKWIFAFTLLLLLVLNVIVYMQFQKPFLAINITLS
                     AFLLIFVMFFFRNPRRVVEIDDPSLIVAPADGTVVVIEPTDEFEYFGDKRIQVSIFMS
                     IFNVHANWYPVAGKVIKTSHHSGRHMAAYLPKSSTENERSTIVIETPSHTQILVRQIA
                     GALARRIVTYAHVGKACHLNEHIGFIKFGSRVDMFLPLDAEIFVEIGESTTGNETIIA
                     RLNED"
     gene            7918..>8144
                     /locus_tag="PKH68_05520"
     CDS             7918..>8144
                     /locus_tag="PKH68_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNX12430.1"
                     /translation="MTLQEAQEQVDGWIKTYGVRYFGELTNLGILTEEVGELARVMVR
                     TYGEQSFKSSDKKGDLADEMADVLWVLLCLA"
     assembly_gap    8145..8244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8263..9147
                     /gene="deoC"
                     /locus_tag="PKH68_05525"
     CDS             8263..9147
                     /gene="deoC"
                     /locus_tag="PKH68_05525"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNX12431.1"
                     /translation="MATKFEELFSLYGCDFITDEQVKQEMELLLSAHFEENNTLEVYK
                     RCFNSIDLTSLKETDTEEEIRSMVEKVNNFEENFPDIPNVGAICVFPAMVSTVKATLT
                     EPIGIAAVSAGFPASQTFIEVKVAETGMCVMEGATEIDVVISVGKFLAGKYDEVFEEL
                     TEIKSACRDAHLKVILETGALKTAANIKKASILAMAAGADFIKTSTGKIPVAATPEST
                     FIMCSAIKEWNEKTGAKVGYKPAGGIATAEDAVKHYTLVKEILGDEWLNNKLFRFGAS
                     SLANKLLSSIRQEEVKYF"
     gene            9330..>10464
                     /locus_tag="PKH68_05530"
     CDS             9330..>10464
                     /locus_tag="PKH68_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12432.1"
                     /translation="MKNFTLLFFAMILAAANLFAADLNVVGSFTSWNNNDAAFKMTEI
                     GTTGIYSLEKTLPAGTHEFKVTYSGTWDNSPAGDNRVFTLATEKTVKFYAKLNGTQIL
                     FFSDAQELYVIGAAVGGWDAASMKLMTNTAAEAAYTADVVAGGYKIVVKDKNSNIVWN
                     DITPSDQNVSSTGNHTFKLDFAAFTVTATANGQTTPSLSSLSNSFITVGQDMNTAAWY
                     NANSTAQAENFNAKNLGSISSPLYIGGEITTAPVLDGVSVKMFCQIDELAITEIPLVW
                     ISNNGTTSSKWQTAAGTDIFAGNTLTKGTAYKLKVWFNATHGTTTLWDSNNMANYVAD
                     FTYDITSKVGNQNVSINISGANRTVKATFNDEAQVELFTLSGQL"
BASE COUNT         2835 a         2272 c         2128 g         3029 t
ORIGIN      
        1 gacattcctt tggcaagaat ttcgccgtga ctgcggtgac ttccgaatat aaagtcatca
       61 acggttaatg tccaggccat tcctactgct gccgactcct gcccgatgga taagtgtgcc
      121 gggccgggat ggttgtaagg cgttccgttg tattctcctt tgatcttgat aagattgagc
      181 atggtttcaa actcacgaat caatgccata tcatgataaa tagctttgaa ttcatcatcg
      241 gtgaaatttt ccttttcatc ttttaccttt ttctgatact gattgacagg aattggctga
      301 aattctataa aacccggctg acgggatttt tttggatcta gaaattgaat ttttggcata
      361 ataatctgcc ccctaacccc ctaaaggggg aatttttcga gggtatttta agttagttag
      421 tattttattt aaaattttag aattaaatct taatctaaga tgtttttaaa taacgaataa
      481 ttcagaaaaa tgctctttaa ttccccctta gggggttagg gggcaactat actgaaaata
      541 ttgtttcttt aaatatctca ctcacagtcg gatgcgggaa tacaacttcc tgcatggctt
      601 ccagcgtcat ttcctgttcg atagccatac aggctccgta aataatttca ctgcatggat
      661 ttccgagcat gtgcaccccg ataacttcac cgtgtttttc ccccaccagg attttacaca
      721 ggccgttgcc tccctcattt tcagctacaa atcgtccggc aaaagtcatg gggagtttag
      781 ccaccttgta ggcgatacct ttggctttgg cagtttcttc ggtttcgccc acacccgcca
      841 cttcgggatt ggtataaact actccgggga tggcattgta gcgcatggta tcaattcttc
      901 ctgtcaaatt attgaccacg acttcgcctt cgcggctggc ggtatgtgcc aacagcgaaa
      961 atccggtgac atcacccgct gcatatactc cgggaacatt ggttcgcatt ttttcatcta
     1021 ctttgatgcc ggtacgtgtc atttctacgt tcagattttc gagaccgaaa cctttggtaa
     1081 ccgctctgcg gccaacgctt atcaatattt tatcgccttc aatggtttca gtttttccct
     1141 ctttttcaaa tactacttta ttaccgtcaa tctgaacaac tttggcattg aggttgaatt
     1201 caatcccttt tttggtataa atatcacgca gcatggcaga gatttcgaaa tccagcccac
     1261 ccagaatttc gggaagcatt tctacaattg taactttaac tcccagacta ttatagaaac
     1321 tggcaaattc cattcctatc actccaccgc ctattactac caatgaattt ggcagttctt
     1381 tcagatccag tatttcgcgg ttggtgagga tggtatctcc cgcttcggcc aatcccggaa
     1441 ttggtggaac agatgcttca gaaccggtac atatcagcag gttttttacc agataacttt
     1501 ctccgttaca ggtgacttct acgccctttt cgcttcgacc ctgtatcatg gcttccccgt
     1561 caatgaccgt tacgttgttc tctttcattt tacttttcac tcctgccacc aactttcgga
     1621 ctattttatt tttacgggcc agtatttttc catagtcaaa gcgaatattt tcaccataca
     1681 caccgtattt atctccgtgt aatgcatttt catacgtttt ggcactgtat agcagggttt
     1741 tggtcgggat acatccttca ttgagacaga cgccacccat tgattttttt tcaaacaaaa
     1801 caacctgaag tccatggtga ccagcacgct ctgctgccac atatccggcc ggaccaccgc
     1861 caataattaa taagtcgatc atataaattt gtgattatgt gtgttttaga ttttcatata
     1921 tattagctca aaattacata aaaattactt caccctgtca actttcatca agtcttgttg
     1981 ctaaaaaaca tctaattcca gattttcaat ttcaacagca atttgtttaa caaaacgagt
     2041 tgcttcaccg ccgtccagtg cccggtgatc atacgtgaga ctgaggccga tatagggaac
     2101 aaatgcatac acgccatcgc ccaggtcttt ggggcgcgga acaatcgtgt tcactcccag
     2161 aatagcagtt tgaggaagat taattaccgg tgtaaacatc tcgacaccat aatttcccag
     2221 attcgaaacc gtaaatgttg ctgcttcggc cgacaggagt tcgggattaa tgctgccttt
     2281 ccgacaagca tttgctattt ctttgaactg atttgccagt ccacttattg acaaatcatc
     2341 ggcattgcgc actaccggta ccattagtcc gcggtcggta tccacggcaa gcgccaaatg
     2401 tattttattg aacaagcgca tggtatcacc caaaaaatga ctgtttacat tcgggaattt
     2461 tttcagcgct ttaattacag caaaacaaac catgtcattt agtgtgatat tggttgcaat
     2521 tttccctgct tccatagcgg atttcacttg tttgcgaagt tcaagtatgc gacgtgcatc
     2581 ggctcccaaa tgatgggtga gctgggctga attctgtaac gaagcatgca ttgatttcgc
     2641 tatgatttta cgcatattcg aaatcttttt gtcgttgtaa tccagtccgt aagtttcgtt
     2701 tgtgagcgat gacagcaaat cggtcgattt ggctgttccg gcaagtccag agccagttcc
     2761 ttgtggttga agtcccgatt cggtagcaat ttttcttgcc aggggagtga gtttcggctg
     2821 attttcaagt actttctcga tatccctttc aacaatgcgc cctttgggtc cggttccttc
     2881 tatttgtgta atatttaccg cctcccggct tgccaaaatc ctggcccggg gagaaatgtt
     2941 actgccgaat tccaaacctt caacactccg gatggttacc gtttcggtta ttgaacttcc
     3001 actttgtgtt atgttattgg ctgcaaaggt ttctggcatc tcagctttgg gtgcagcggg
     3061 ggcattccct ccaatcaaag ccacatactc ttcgccaggc tgaccgatta ccatcatatt
     3121 ttccagtacc agcacttcgt cgccatcgtt atagagacat tccaaaacca caccatcagc
     3181 aggggattct tcttcgaacg atgccttatc ggtttcgtaa gtaaacagga tatcaccttt
     3241 ttttactgca tcaccctttt tcttcaatat ctccgtaatg atacaactct ctactgattg
     3301 gccctgtttg ggcattacaa caactactgc catggatttt tctttttttg attttaattt
     3361 aatggtttaa gctttgtatt aaaacccgac attaacaaca aatcaggaat catttttttc
     3421 aaaatcggta ttcaaattta ttcattttta ttcgaaaact tttcaacgaa caaaagtaaa
     3481 atttatataa ccaacaatca gtatattaaa tgattttaat gcagtgattt ttgacaaaaa
     3541 cagttgcatc cggttttttc acttgttgaa acccggcgct tttgttttta tttgtggggg
     3601 ggtatgatgt ttatattgtg taattacgaa aataactctt tgttttacac cgattctgct
     3661 ccaggagcat tcatgccgct aaaataaaaa aattgggata ttgatatcag cttactgaat
     3721 ttgtctgaaa gtgatcaaat gtgaaaacga aacccataga aagttgaagg ggagttgtat
     3781 gtcctataat aaaaaacata tcgctatctt cagtctgctc tgcattgaaa cagcctcaga
     3841 gggaaggttg agaagaaata tcacaaagga atccgaaaat ttcagtgtga agtagcttat
     3901 gaaatttgga aaaaatcaag ccaataagct gactaaaaag gcatattaat cgctattgta
     3961 agcagaaaac tattttttta tggccaaccc ggtgcttcaa tcagaacgac tgcatatcca
     4021 ccagcctttt gtagtagcct cccagtgcca tcagttcttc atgttttccc tgctctacga
     4081 tttttccctc gtgaagtaca cagatgaggt ctgcgcgttt aatggtggac agacggtgcg
     4141 ctacaaccac cgtagtacgg tttttcatca gattatcgag cgcttcctgc accatatatt
     4201 ccgattcggt gtcaagggcc gaagtggctt cgtccaggat caggattgga ggatttttga
     4261 ggatggcacg ggcaatactg atgcgctggc gctgccctcc cgagagtttg ctgccccggt
     4321 caccaattgt gctttcgtac ccattttcgg tagccattat aaaatcgtga gcattggcta
     4381 ttctggctgc attttcaacc tgttcggggc ttgcctgcga tactccaaag gcaatattat
     4441 tataaaaagt gtcattaaaa agtattgcct cctggtttac attgcccatc aatgaacgaa
     4501 tgtcggattt acgaaaatcc ttgatattta caccgttaaa aaacacccct ccttcggcag
     4561 ggtcgtaaaa acgcggtaga agatcaacca gcgtggattt accggagccc gactgcccga
     4621 caattgccac tgtttttccc aggggaatgg tcagattgat gttttgcaac acccaatccg
     4681 actgatactt gaaactcaga tttttgaatt caattttttc aactatatgt ggcaattctc
     4741 tcggggtagc cgggtcggga atattatttt cctgcatcag aattttatca atccggtgaa
     4801 gtgacgccag acctcgctga atgctgtatg atgcttttga gagttccttg gcaggtggaa
     4861 caatgctgta gaaaattacc agataataaa taaactgggc tgcaccgatg ctgctgttct
     4921 gttcaagtat aagacttccg ccaaaccaaa tgagaatagc aattactatg gttccgaaca
     4981 attcgcttac agggtgtgcc aggttgtatt ttcgatgaat gcggttgaaa gtatggcgga
     5041 ttttttcgtt caggtggcta aaacgaacag taagtttctc ttcagcattg aaagccttta
     5101 caatacgcaa tccgccgagg gtttcttcaa tctgagaaag cagttcgccg gtttgttcct
     5161 gcccaatggt tgaacgtttc ttgagcgagc gcccgataac tcctatcaac caggcactta
     5221 tgggcaacag aaccattaca aaaagtgtta attcccaact cattgcaaat agtacggcca
     5281 cataaatgag gatcatcacc ggatttttaa gcaccatatc cagcgagctg atgatggatg
     5341 cttccacctc caccacatcg cccgtcatac gcgacatnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnngtc aatccctcat tccagttcca gggaatatac actgtatccg
     5521 atttagtcaa cccgaaaagt atctgaagca ccggaataat ggctgcaaat gaaaacaggc
     5581 tgaatacagt ggaaattaaa ttgaaagcca aacttagaaa aatatatttc ttgtatggcg
     5641 gagcaaaccg cttgataagc gtcagaaaat caaacatttt gttgttttag gattgaaaat
     5701 atattttttt tgaaaatcaa attccggtgt aattacgcgg tgtaatccgg cgaagttctt
     5761 cctttacggc agcactcaca tcgagctggt caatgaattc ccggaatgat ttttcattaa
     5821 tggcttcgtt ggtacgggtg agggctttca gggcttcgta gggagcggga tattcctcac
     5881 gccggagaat agtctgaatt ccttcagcca ccactgccca ggtattgtcc aggtcacggt
     5941 aaagagcggt ttcgttgagt aacagtttat ccagtccctt gagtatgctt tgggtggcaa
     6001 tgagtgtatg tgaaaagggt acacccacat tgcgcagcac ggtgctgtcg gtgaggtcgc
     6061 gttggaagcg cgaaacgggc agcttttggg ccaggaaagt aaatatggca ttcgccattc
     6121 ccagattacc ttcggcattt tcaaagtcga tcgggtttac tttgtgcggc atggccgatg
     6181 aacccacttc gccggcttta attttttgtt tgaaatattc catggaaaca tagctccaga
     6241 tatcgcggca gaagtcggtg agaatggtgt tgatacgtgc cagtgcgtca aacaaagcag
     6301 ccaggttgtc gtaattggag atttgagtag tccactgctc ccgctccagt cccagttttt
     6361 cgtgggtaaa tttattggca aacgatcgcc agtcgatggc aggataagcc acatgatgtg
     6421 cattgaagtt gccggtagca cctccaaact tagcggaatg tggtaccatt cggagtaatg
     6481 tgagttgctg atttaggcgg ctcacaaaaa ccataatctc cttacccagc cgcgtgggcg
     6541 atgccggttg gccgtgcgtt ttggccagca tggatacatc ccgccactca tcggccatac
     6601 gcgtgagttt ctttatcaaa ctgtccagtt caggataata cacctcttcc atcgcttttt
     6661 tgatggatag cgggacagaa gtgttgttta tatcctgtga agtaagtccg aaatgaataa
     6721 attccttaaa agaacccaga ccgatttcat caaatttttc cttcagaaaa tattccaccg
     6781 cctttacatc gtgattagtc actttttcta tttccttgat tttttcggca tcttcgaggc
     6841 taaattgacc gtaaatttcc ctcagtttag gcagaatatt tgaaggtacg gattcaagtt
     6901 gcttcaacgg caattcacaa agtgcaataa aatattccac ttcggtgagt acacggaaac
     6961 gaataagcgc aaattcggaa aaataggccg aatacggttc tgtttttgaa ctgtagcgcc
     7021 catcgacagg tgaaatggca gtgagtgagg tgattgacat attgtaggct gattgatttc
     7081 tacggttttt tcattcagga atgaaggtac aaagttaatc atttttgaga gaattgaaaa
     7141 tatcacccgc ctgcttgttg gattaatatt aaatgcttac ttttgttagt tattatcaac
     7201 cgttcatttt tgaaaatttt ctaatatttc cccttattag tcaatgaaat acattaaaat
     7261 acacaaagaa ggcaaatgga ttttcgcatt caccctgctg ttgctcctgg tgctcaatgt
     7321 gattgtttac atgcaattcc aaaagccctt tcttgccatt aatatcacct tatcggcttt
     7381 tttacttatt ttcgtcatgt tctttttccg aaatccgcgc cgcgtagtcg agattgacga
     7441 tccaagcctg atagtagctc cagccgacgg caccgtggtg gtgatagagc caaccgacga
     7501 atttgaatat tttggcgaca aacgcattca ggtgtccatt tttatgtcca ttttcaatgt
     7561 tcatgccaac tggtatccgg tagccggaaa agtgattaaa acctcgcatc acagcggaag
     7621 acacatggct gcctatctgc ccaaatcaag cacagagaat gaacgttcga ccattgtgat
     7681 cgaaacccct tcccatactc agattttagt gcgccagatt gccggtgccc tcgcacgccg
     7741 gattgtaacc tacgcacacg tggggaaagc ctgtcatctc aacgaacaca tcggttttat
     7801 caaattcgga tcaagagtgg atatgttttt accacttgat gccgagattt ttgtggaaat
     7861 aggtgaatca accaccggca acgaaactat tattgcacgg cttaacgaag attgaaaatg
     7921 acactacagg aagctcagga acaggtagac ggatggataa aaacctacgg ggtacgctat
     7981 ttcggcgaac tcaccaacct gggcatactc accgaagaag ttggggaact tgcacgggtg
     8041 atggtgagaa cctatggtga acaatcgttt aaatcatccg acaagaaagg cgatctggct
     8101 gatgaaatgg ccgatgtact ctgggtactg ttatgcctgg ccaannnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnaaatag taaataacac ttatggcaac aaaattcgaa
     8281 gaacttttca gcctctatgg ctgtgatttc attactgatg aacaggtgaa acaggaaatg
     8341 gaactgctgc tttctgcaca tttcgaagaa aataacaccc ttgaagttta caaacgctgt
     8401 ttcaacagca tcgacctcac ttcactcaaa gaaaccgata cagaagaaga gataaggtca
     8461 atggtggaaa aagtaaataa ttttgaggaa aattttcctg acattccaaa tgtcggtgct
     8521 atttgcgtgt ttccagccat ggtttcgacc gtgaaagcta ctttgacaga accaatcggc
     8581 attgcagccg tttcagccgg attcccggct tcacaaacat tcattgaagt gaaagtggcc
     8641 gaaacgggca tgtgcgtgat ggaaggagcc acagaaattg atgtggtcat ttcggttgga
     8701 aaatttctgg caggcaaata cgatgaagta ttcgaagaac tcaccgaaat taaatcggct
     8761 tgccgcgacg ctcatctgaa agttattctg gaaaccggtg cccttaaaac cgccgccaat
     8821 attaaaaagg catcgatact tgccatggct gccggagccg atttcattaa aacgtcaacc
     8881 ggcaaaatac ccgtagctgc cactcccgaa tccaccttca tcatgtgctc agccattaag
     8941 gaatggaacg aaaaaaccgg agcaaaagtg ggctacaaac ccgccggagg aatagccaca
     9001 gctgaagatg ccgtaaaaca ctacaccctg gtaaaagaaa ttctgggcga cgaatggctc
     9061 aataataagc tgttccgatt cggagccagc agcctggcca acaaactgct ctcttccatc
     9121 cgacaggaag aggtaaaata cttttgataa ttcgtttttg tagttaattt aaagccggca
     9181 gttttgttgc cggctttttt ttgtcactgt ttcaatcgtt tgcgttatga aacgttggat
     9241 tctcagttga tttttcatat tgaaataatc taacatttct ttctttgggc gatatttaaa
     9301 taccccaaac aattaaaaat taatagatta tgaagaattt tacgctttta ttttttgcaa
     9361 tgattttggc agctgctaat ctttttgcag ccgatttgaa tgtagtggga tcattcacca
     9421 gttggaataa caacgatgcc gcattcaaaa tgaccgaaat cggtactaca ggcatttatt
     9481 ctcttgaaaa aacactaccc gcaggaacac acgaatttaa ggttacatat tccggaacat
     9541 gggataattc acctgccggt gataacagag tttttactct tgcaactgaa aaaacagtta
     9601 agttttatgc caaattaaac ggaacacaaa ttcttttctt ctccgatgct caggaacttt
     9661 atgtcatcgg agcggctgtg ggtggctggg atgctgccag catgaaactc atgacaaata
     9721 cagctgcaga agctgcctac acagctgatg tggtagccgg tggttataaa attgtagtta
     9781 aagacaaaaa cagtaacatt gtatggaatg acatcactcc gtctgatcaa aatgtgagca
     9841 gcaccggaaa tcataccttt aaactcgatt ttgctgcttt tacagttact gccacagcca
     9901 atggtcaaac aactccttca ctttcaagcc tttccaactc tttcatcacc gtaggtcagg
     9961 atatgaacac tgccgcctgg tacaatgcaa actctacagc tcaagctgaa aatttcaacg
    10021 ctaaaaatct gggttcgatt tcaagtccgc tttatattgg tggtgaaatt acaaccgctc
    10081 ccgttcttga tggcgtaagt gtgaaaatgt tctgccagat tgacgaactg gctattactg
    10141 aaatacccct ggtatggata tccaacaatg gcacaacctc ctccaaatgg caaacagcag
    10201 ccggtacaga catattcgca ggaaatacac tcactaaagg aactgcttac aaattaaaag
    10261 tatggttcaa tgccacccac ggcacaacaa cgctgtggga cagcaataat atggctaatt
    10321 atgtggcaga tttcacatac gacattacat caaaagttgg taatcagaat gtttcaataa
    10381 atatttctgg tgcaaaccgc actgtaaagg ccactttcaa cgacgaagct caggtagaac
    10441 ttttcacttt gtcgggtcag ttga
//
LOCUS       DAOPZK010000068        10244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_7621_length_10244_cov_24.396702, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000068 DAOPZK010000000
VERSION     DAOPZK010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_10244_cov_24.396702"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..703)
                     /locus_tag="PKH68_05535"
     CDS             complement(230..703)
                     /locus_tag="PKH68_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNX12433.1"
                     /translation="MDALLIFLGVLCIIAGIIGSVLPVLPGIPLSYGGILLLHFTEKI
                     QFSVEFLIFWAIIVIAVSLLDYYIPIWGTKKFGGSKRGVWGCAIGMVVGIFLGPWGII
                     IGPFAGAVIGELTGGKQSTAALKAGFGSFLGFIAGVATKLVVGGFLLFYAIKEII"
     gene            820..1665
                     /locus_tag="PKH68_05540"
     CDS             820..1665
                     /locus_tag="PKH68_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNX12434.1"
                     /translation="MMEGINKHIVSEIKEIFTNKPYNLGIALSGGGIRGMCHAGVMKA
                     LEEYGIKPDIIAGVSAGAIVGALYADGYSPDDIAGFFEHVAFRKMTKVRIPNGGLFGI
                     ESFEKFLAGKLRAKTFEELKIPLRIVATNLDRGESVVFTSGNLLDAIIASSTFPVLFT
                     PKVINGENYIDGGVFKNFPVSIIRDDCEKIIGINASPLVADKYKMSVLNVAIRSYNFM
                     FKANILHDKELCDFLIEPVDMANYETFETEKSREIYELGYQTAKDMIEGRLKENKNKK
                     QFQYE"
     gene            1777..2970
                     /locus_tag="PKH68_05545"
     CDS             1777..2970
                     /locus_tag="PKH68_05545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX12435.1"
                     /translation="MLSVSERLASLAPSATLAMSQKSNELKAQGIDVINLSVGEPDFN
                     TPDHIKAAAKKAIDDNFSFYSPVPGYPALRKAICEKLKTENGLDYTPEQIVCSNGAKQ
                     SVCNVILAVIGKGDEVIIPAPYWVSYPEMVKIADGTNVIVSAGIEQDFKITPQQLEEV
                     ITPKTKAIILCSPSNPTGSVYSKDELKGLADVLAKYPDIIIISDEIYEHINYGGAHES
                     IAQFENVRDRVVIVNGVSKGYAMTGWRLGWIAAPKWIASACNTLQGQYTSGPCSISQK
                     ASEAAYTGDQTCVGEMQKAFERRKNLVVKMAREIPGLKTNEPKGAFYIFPDCSAYFGK
                     SFNGTTIHNPEDLALFLLAEAHVACVGGTDFGAPTCIRMSYATSDENLVKAFGWIKEA
                     LAKLS"
     gene            3150..4883
                     /locus_tag="PKH68_05550"
     CDS             3150..4883
                     /locus_tag="PKH68_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12436.1"
                     /translation="MKKNYLITIAVFLSTSVFAQLALVTTFTTDNEPKQEQIREDNYF
                     KGHLYYFGQGSATSSQLYKTDGNTITLVKDFGPVVPEGETSSGISISNFNQTSDKLFF
                     TRFSFSAKSDAFTELWASDGTTAGTVKIASYRYAGTGRFGFVLNGESNGTYHKYQNSI
                     GNKLILNAYNPDDPGTTLIMWVSDGTAAGTKPLLDNSGGKVYGGVFFGTRMNGKYYFG
                     ARPQNSIDPGGYLYETDGTNAGTKKVNSGLFYMSSNTSEPLNGKMLFWANTVNENYGL
                     WESDGTDAGTKPFAGLPGTNGKKTYYQLGLDLANDGKQVYFTIQPTAAVRTAEVWATD
                     FTPGNTRKIRDDSKLLPNKIMLGNGYAYVEYWDYSFGTNSQYQLGYTDGTALGTKTLL
                     EKGASSSSFGLYQNWLYFPHYESKNGYLFSNVELWSSDGTAGNTKMIHDIYPGEVTFY
                     NPPFKYSSSPRSFFNLEDDLYFVANDGNNKNLYRLKGSGTSVATVGSGKNAGLLHVFP
                     NPCKESVNVSSAGNEEWTLTDLQGKVVMRTMLNEGDNALNVKELSNGVYLGIIRNKTN
                     GATSVGKIIKE"
     gene            5025..6941
                     /locus_tag="PKH68_05555"
     CDS             5025..6941
                     /locus_tag="PKH68_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNX12437.1"
                     /translation="MKLKLLFTLFFVFFGASIYAFTPKADAYLQQYRKAAYSETGLKA
                     LIDFCGEYKTYPYDTLKKYASEALLLSRRLQNENFELQSKFWMATYFYRTSQLDSALN
                     ICNQLEKSKIRNVPIDRKNKLLISSILIKKGQNQEAISRYFKLLEESDIQKDTLSYLS
                     AMHGLGWANMEINRTDEAKKWLLNVLNMPVSSRWEEIKIPSYINLSTILGIQNNLDSA
                     NTLIQKAIELSGKFNNLLNEANSYNILANIYSARGWNQKAIDAVKKSMQIRLRIGDPF
                     YIVSDLAQLSGMYAEMGNYTEGISLALKAWEMTNEKRLASKKPLVLDALFKNYYGSGN
                     YKKSTETLLLIDKIKDSTNIAVSAEKISEIEAKYELSKKEITIKNQENEINRKNLIIL
                     FALISLIGILITGFVIYRSLQYIQQTRIKTIELEQQQKLTQSIFETEENEKTRIGASL
                     HDGLGQLLSAIKMNMQFIQNHLNPDNRRVDEVYNKTLDLVEASIRETRDVSHELVTNF
                     IISKGFKTAIEDLVNKPNGSGLEFEISILDIPNTVSDTIKINVYRIIQECINNILKHA
                     HATKVYITLEADEEYIHCIIEDNGQGFDLQTVKKNGIGIDNICTRVHFLKGKIDITSH
                     PGNGAMIAFHIPME"
     gene            6998..7645
                     /locus_tag="PKH68_05560"
     CDS             6998..7645
                     /locus_tag="PKH68_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX12438.1"
                     /translation="MPQNQINIAILDDHQIIIDGLKLLLESDSNINVVFEATSGSIFL
                     RKLTEIDRIVDIVLLDLMMPEISGFEMALMLKKDYPEIKIIVLSMNIDGKSITELKAK
                     AGIKGFLSKSVDKTELMLAIMTVNSGEEYFSQEAEREMDNYEKISKELEQITLTKREI
                     EIIRLIDKGLINKEIAKKLFISEQTVTTHRKSILRKTGAHNVSTLLNFARKAHII"
     gene            7793..7987
                     /locus_tag="PKH68_05565"
     CDS             7793..7987
                     /locus_tag="PKH68_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNX12439.1"
                     /translation="METKKKLVRSKNTMIAGVCAGIAEYFGMDVTLTRILYVLVSVFS
                     AAFPGIIVYLILMLIMPKAE"
     gene            8011..8223
                     /locus_tag="PKH68_05570"
     CDS             8011..8223
                     /locus_tag="PKH68_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNX12440.1"
                     /translation="METEKKHFKRSEDRMLAGVCAGIAEYFGIDVKITRIVYFGFTIL
                     TIFFPGIILYLALMLLMPREGNSPTP"
     gene            8353..9195
                     /locus_tag="PKH68_05575"
     CDS             8353..9195
                     /locus_tag="PKH68_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNX12441.1"
                     /translation="MILSNYHSHCIFCDGRSSMEDFIRFAIAKGVTKYGFSSHAPLPF
                     HTSWNMDLDDLAYYKLEFFRLKEKYAAEIELFLGLEVDYIQDVIDAHSDLYDTPDFDY
                     KIGSVHYLDPMPEGGFFSVDGKLYNFQKKMNEIYRGDIRMVVERFFEISSNMVRKGGF
                     DIVGHMDKISQNGMNCKGFDITQPWYRKLATDLLQLIKDKGLMLEINTKSFAELGITY
                     PDVQFFPVIREMGIPILVNSDCHYPDKILDGFGPVYELLIQAGFQSKMELTEKGWVES
                     PLTP"
     gene            9340..>10244
                     /locus_tag="PKH68_05580"
     CDS             9340..>10244
                     /locus_tag="PKH68_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNX12442.1"
                     /translation="MNFKLISPYRPTGDQPEAIRQLVDGINMGVPYQTLLGVTGSGKT
                     FSVANVIEQVQKPTLVLSHNKTLAAQLYSEFKSFFPENAVEYFVSYYDYYQPEAYLPT
                     TDVYIEKDLSINQELEKLRLATTSALLSGRRDVLVVSSVSCLYGIGNPDDFTENVIEI
                     RRGQKIVRNVFLRSLVDSLYTRNEIELNRGNFRVKGDTVDIFLAYTDFILRVVFWGDE
                     IDEILTVETIDYHVIESFESYKIYPASIFVTTKPRIMNAIQMIESDLEVQVGNFRAAG
                     KDYEAKRLYERVTYDLEMIKELGYCS"
BASE COUNT         3286 a         1992 c         2159 g         2807 t
ORIGIN      
        1 tgagctgaaa agttttgccc gaaccggcag aagcacgata tatgtttagc atggaagccc
       61 cctaaccccc tcaaggggga atttttgatt tttgatttat attgattttg ttgttttttc
      121 agcaattatt ggaacatttt tctttttaaa tgtttacttt cctgtaagta ctgctttgct
      181 cataatttgc actcaccaaa gtcccccttg agggggattt agggggtttt tatattattt
      241 ctttaatcgc ataaaacagc aaaaatcctc cgacaaccag tttggtagca acaccggcta
      301 taaatccgag aaaggagcca aagccggctt tgagcgctgc ggtagattgt tttccaccgg
      361 tcagttcacc aatcaccgca cctgcaaacg gaccgataat aattccccat ggacccagaa
      421 aaatgcccac caccattcca atggcacatc cccaaactcc ccgtttgctt cctccgaatt
      481 tttttgttcc ccaaatcgga atataataat cgagcagtga aacggcaata acaatgatag
      541 cccaaaagat aagaaattcg acagagaatt gaattttttc cgtaaaatga agcagcagaa
      601 ttccgccgta gctcagtgga attcccggta aaacaggaag tacagaacca ataatgcctg
      661 caatgataca caaaaccccc aaaaaaatta atagcgcatc catatcaaac caatttgtaa
      721 taagtgtcac acaaagatag aaataaaaag aggtttgaaa agaaaaatgt ataaatttgt
      781 agaggaatta aatggtaaca attcaacctg gaacagtgta tgatggaagg aattaataag
      841 catatagtca gcgagataaa agaaatattc accaacaaac catataattt gggaattgcc
      901 ctgagcggtg gcggcattcg cggaatgtgt catgccggtg taatgaaagc actcgaagaa
      961 tacggcatta agcccgatat aattgccgga gtaagcgccg gagccattgt aggggcgctc
     1021 tatgccgatg gatattcacc cgatgatata gccggatttt ttgaacatgt ggcatttcgt
     1081 aaaatgacca aagtaagaat tccaaacggt ggactgtttg gcatcgaaag tttcgagaaa
     1141 tttctggccg gtaagttgcg ggcaaaaacc ttcgaagagt tgaaaattcc attacgcatc
     1201 gtagctacaa acctggacag aggcgagagt gtggttttca ccagcggaaa tctcctcgat
     1261 gccattattg catcatccac atttccggtg cttttcaccc cgaaagtaat caacggcgag
     1321 aattacatcg acgggggcgt gttcaaaaac tttccggtga gcattatccg cgacgattgt
     1381 gaaaagatca tcggcatcaa tgccagcccg cttgtggccg ataagtataa aatgtcggta
     1441 ctcaatgttg ccatccgttc atataacttt atgttcaaag ccaatatcct gcacgacaag
     1501 gaattgtgcg attttctgat tgaaccggtt gatatggcca attatgaaac atttgaaacc
     1561 gaaaaaagcc gtgaaattta tgaacttgga tatcaaaccg ccaaagatat gattgaagga
     1621 agattaaagg aaaataaaaa taaaaaacag tttcagtacg aatgacaaaa aagaactatt
     1681 taacgaataa atagtgttgg aatgaacaaa aagtgtggaa aaatgaatta ttttgattaa
     1741 ttttgtaccg aatttcaaaa aaaaaacgat aaaattatgc tatcagtatc agaacgttta
     1801 gcttcgctcg caccttcggc aacacttgcc atgtcacaaa aaagcaacga gcttaaggca
     1861 cagggaattg atgtgattaa tctgagtgtc ggtgaacccg attttaatac accggatcat
     1921 ataaaagctg ctgcaaaaaa agcgattgat gacaattttt cgttttattc gcccgtaccc
     1981 ggatatccgg ctctgcgcaa agctatctgc gagaaactta aaaccgaaaa cggtctggac
     2041 tacacaccag agcaaattgt atgttccaac ggagcaaaac aatcggtttg taacgttatt
     2101 ctggccgtaa ttggcaaagg cgatgaagtg attattcctg ctccttattg ggtaagctac
     2161 cccgaaatgg tgaaaatagc cgatggtaca aatgtaatcg tttctgccgg aatcgagcag
     2221 gatttcaaaa ttactccgca acaactcgaa gaagtcatca ctcccaaaac caaagctata
     2281 atactttgct cgccttccaa ccccaccggg agtgtttatt ctaaggatga gctgaaaggg
     2341 ttggccgatg tacttgcgaa atatcccgat atcattatta tttctgacga aatatacgaa
     2401 cacatcaact acggtggtgc acacgaaagt atcgctcagt tcgaaaatgt acgcgaccgg
     2461 gtagtgattg ttaacggtgt gtcgaaagga tatgccatga ccggatggcg tctgggctgg
     2521 attgcagcac caaaatggat tgcctcagcc tgcaatacct tacagggaca gtacacctct
     2581 ggtccgtgtt ctatttccca gaaagcttcg gaagctgcat atacgggcga tcagacctgt
     2641 gtgggtgaaa tgcagaaggc attcgaacgc cgcaaaaatc tggtggtgaa aatggcacgt
     2701 gaaattccgg gtctgaaaac aaacgaaccg aaaggtgcct tctacatttt ccccgactgc
     2761 agtgcctatt tcggtaaatc cttcaacgga accaccatcc ataatccgga agatttagcg
     2821 cttttccttc ttgccgaagc tcacgtagcc tgtgtgggtg gaaccgattt tggagcgccc
     2881 acatgtatcc gcatgtctta tgccacttcc gatgaaaatt tggtgaaagc tttcggatgg
     2941 attaaagaag cattggcgaa actctcatag gcattataca ctattccggt agctttttcc
     3001 cgggaataat aaaagcgaag taacggattt ctgttgcttc gttttctttt ttaaaaatac
     3061 ccatttcagg gtattgattt catattatta gtacaataaa tttgcaaaag tgaaattgaa
     3121 acaaaaattt taacttaata tgaattaaaa tgaaaaaaaa ttacctgatt acaattgctg
     3181 ttttcctgag cacaagtgtg tttgctcagc ttgccttagt cacaactttt acgactgata
     3241 atgaaccgaa acaggagcaa atccgtgaag acaattactt caagggtcat ttgtattatt
     3301 tcggacaggg aagtgctaca agcagtcaac tctataaaac agatggaaac acaataactt
     3361 tagtgaaaga ttttggtccg gtagttcccg aaggcgaaac ctcatcgggt atttccattt
     3421 cgaattttaa ccaaacttca gataaacttt tttttacacg cttttcattt agtgccaaat
     3481 cagatgcttt tacggaattg tgggcttccg atggtacaac tgcaggtaca gtcaaaatag
     3541 cgtcataccg gtatgcggga accggcaggt ttggctttgt gctcaacgga gagagtaacg
     3601 gcacatacca taaataccaa aacagcattg gtaataagct gatattgaat gcttacaatc
     3661 ccgatgaccc cggtactaca ctcattatgt gggtttcgga tggaacggct gcaggtacca
     3721 aaccattgct cgacaatagt ggcggaaagg tgtacggtgg tgtgtttttc ggaacccgaa
     3781 tgaacggcaa atactatttt ggcgcccgtc cgcaaaattc catcgacccg ggaggatatc
     3841 tgtatgaaac cgatgggaca aatgccggaa ccaaaaaagt gaattccggc ttgttctaca
     3901 tgagttcgaa cacaagtgaa cctctgaacg gcaaaatgtt gttctgggcc aatacagtga
     3961 atgaaaatta tgggctttgg gaaagcgacg gaactgatgc cggtacaaaa ccatttgcag
     4021 gactgccggg aacaaacgga aaaaaaacgt attatcagtt gggacttgat ttagcaaatg
     4081 atggcaagca ggtttatttc actattcagc ctacagcagc agtcagaaca gccgaagttt
     4141 gggcaaccga ttttactccc ggaaacaccc ggaaaataag agatgattcg aaattattac
     4201 caaacaaaat tatgttaggg aatggctacg cttacgttga gtactgggat tacagttttg
     4261 gtactaattc acagtatcaa cttggataca ccgatggcac cgctttgggt acaaaaacgt
     4321 tattagagaa aggggcaagc tcaagcagtt tcggtttgta ccaaaattgg ttgtattttc
     4381 ctcattatga aagcaaaaac ggttacctgt tttccaatgt tgaactttgg agctctgatg
     4441 gtacagccgg aaacacaaag atgatccacg atatctatcc cggtgaagtt acattttata
     4501 atcctccgtt taagtattct tcttctcccc gttcattttt caatctggaa gatgatttgt
     4561 attttgttgc caatgatggg aataacaaga atttgtatcg gctcaaaggc agcggaacat
     4621 ccgtagcaac tgttggttcc ggaaaaaatg ccggcttgct gcatgttttc cccaatccgt
     4681 gtaaagaatc cgtaaatgta agctccgccg gtaatgaaga atggacacta actgatttgc
     4741 aaggcaaagt tgtgatgcga actatgctga atgagggtga taatgcgttg aatgttaaag
     4801 agttgtctaa tggagtttat ttaggaatta ttcgcaataa aacgaacgga gctacttctg
     4861 tgggaaaaat cattaaggaa taaccaatcc ggattcagtc aacatgcaat aataaatgcc
     4921 ttgtttcaga acattttaat gcatttctga gtttattact tcataccaac agaaatatat
     4981 tttaactgaa aatcccgaca atctctcatc ttattttctg aagtatgaag ttaaaactgc
     5041 tattcactct ttttttcgta ttttttggtg cctccatata tgccttcaca ccgaaagcgg
     5101 atgcatatct gcagcaatac aggaaagcgg catatagcga aacgggtttg aaggcattaa
     5161 tagacttttg cggcgaatat aaaacctatc cgtatgatac gctgaagaaa tatgcttcag
     5221 aagcgctgtt gctttcacgt cgattacaga atgaaaactt cgaattacaa agtaaattct
     5281 ggatggctac ttatttttac cgcacaagcc agctggatag tgccttgaat atctgcaatc
     5341 aactggaaaa aagcaaaatt cggaatgttc ccatcgaccg gaaaaacaag ttgttaatta
     5401 gctcaatcct gataaaaaaa ggacaaaacc aggaagcaat aagcagatac tttaaattgc
     5461 tggaagaatc ggatattcaa aaggatacgc tttcgtattt atctgcaatg cacggtttag
     5521 ggtgggcaaa tatggaaatt aatcgtacgg atgaagctaa gaaatggttg ttgaacgtac
     5581 ttaatatgcc tgtatcttcg agatgggaag aaataaagat tcccagctat ataaatctga
     5641 gtacaattct tggcatacaa aataatctgg attcggctaa cacgctgatt cagaaagcaa
     5701 ttgagctttc cgggaaattc aacaatctgc taaacgaagc caattcctat aatatattag
     5761 ccaatattta ctcagcccgc ggatggaatc aaaaagcaat cgatgcagtg aagaaatcca
     5821 tgcagattcg tctccggatt ggcgatcctt tctatatcgt ttcggatctg gctcagcttt
     5881 ccggcatgta tgccgaaatg ggaaattata cggaaggtat cagtttggca ctaaaggcct
     5941 gggaaatgac caatgaaaag cggcttgctt ccaaaaaacc attagttctt gatgcgctct
     6001 tcaaaaacta ttatggttca ggtaactata aaaaaagcac agaaacattg ctgctgattg
     6061 acaaaataaa ggactcaacg aatattgctg tttctgcaga aaaaatttca gagatagaag
     6121 caaaatacga actttcaaaa aaggaaataa ctataaaaaa ccaggaaaat gagattaacc
     6181 ggaaaaatct gatcatcttg tttgccctaa tttcgctcat cggaattcta attacaggtt
     6241 ttgtgattta tcgctcactc cagtacattc agcaaacaag aattaaaacc attgaactcg
     6301 aacagcaaca aaaactgaca caatcaattt ttgaaacgga agaaaacgaa aaaacccgca
     6361 ttggggcctc gcttcacgat ggactcgggc aattgcttag tgccataaaa atgaatatgc
     6421 agtttataca aaatcacctg aaccccgata accgcagagt ggatgaggta tataacaaaa
     6481 cacttgattt ggtggaagct tctatccggg aaacccgcga tgtatcgcac gagctggtaa
     6541 ctaattttat tatttccaaa ggattcaaaa cagctattga agatcttgta aataaaccga
     6601 atggcagtgg tttagaattt gaaatttcca ttctcgatat tccgaatact gtaagtgata
     6661 cgattaaaat caacgtttac cggataattc aggaatgtat caacaatatt ctaaaacatg
     6721 cacatgccac aaaagtgtac atcacactcg aagctgacga ggaatacatc cactgcataa
     6781 tcgaagacaa cgggcagggt tttgatcttc agacagtgaa aaagaatggt attggcattg
     6841 ataatatctg cacccgcgta catttcctga aaggaaaaat cgacatcacc tcacaccccg
     6901 gaaatggagc tatgattgca tttcatattc cgatggaatg atatttgctt tttgtaaatt
     6961 ataaatatta aattttctat ctgaaatcca atcatttatg cctcaaaatc aaatcaacat
     7021 tgccattctg gacgaccatc aaatcataat tgacggattg aaactgctgc tggaaagtga
     7081 cagcaatatc aatgtggttt ttgaagctac atcaggcagt atttttttaa gaaaactgac
     7141 tgaaatcgac agaattgttg atattgtatt gctcgatttg atgatgcccg aaattagcgg
     7201 gtttgaaatg gctctaatgc tcaaaaaaga ttatcccgaa ataaaaatta tcgtattgtc
     7261 catgaatatt gatggaaaat ccatcaccga actcaaagcc aaagccggaa tcaaaggctt
     7321 tctgtcaaaa tcagtggata aaaccgaact gatgttagcc ataatgaccg taaatagcgg
     7381 agaagaatat ttcagccagg aagccgaacg cgaaatggac aattacgaaa aaataagcaa
     7441 agaactggag caaataaccc ttaccaaacg cgaaattgaa attatccgat tgatagacaa
     7501 ggggttgata aataaagaaa tagcaaaaaa actttttata agcgagcaaa ctgtaaccac
     7561 acatcggaaa tccatcttgc gtaaaaccgg cgctcacaat gtgagtactc ttctgaattt
     7621 tgcaagaaaa gcacatatca tttaattagg aaatcccaca cattcgaatc caataattac
     7681 ggaatcaact cataaataaa tctctttatc ttttcctgaa aaatccacac tgcctgatga
     7741 aaaatgtttt atctttgtaa caggtaattt ttttaagaaa gaaaactgta atatggaaac
     7801 aaagaaaaaa ctggtacgtt ccaaaaacac aatgatagca ggtgtgtgtg ccggaatagc
     7861 cgaatatttt ggcatggatg taacattaac ccgtatttta tatgtgttgg tgtctgtatt
     7921 ttctgcagct tttcccggaa ttattgttta tttaattctg atgctgatta tgccaaaagc
     7981 cgaatagtaa acctctgaaa aaaggatgat atggaaacgg aaaagaaaca tttcaaacga
     8041 tcggaagacc gaatgctggc cggagtctgt gccggaatag ccgaatattt cggaattgat
     8101 gtaaaaatta cccgcattgt ttatttcggt tttacgatac tcaccatttt ttttccgggg
     8161 ataatccttt atctggcact catgctgctg atgcccaggg aaggaaattc tcctacacct
     8221 tgaatttcag ataattgctc aatatggaaa cattgagaat gaaataactt agctaaaata
     8281 ccttttatta attctataac aattattata tgcttctaat tcccccttga gggggttagg
     8341 gggcttttta ttttgatttt atccaattac catagccact gcattttctg cgacggacgc
     8401 agttcgatgg aagattttat ccggtttgca atagccaaag gggttacgaa atacggattt
     8461 tcgtcgcatg caccgctgcc gtttcatacg tcctggaata tggatctgga cgatttggct
     8521 tattataagc tggaattttt tcggttgaaa gagaaatacg cagctgaaat tgagcttttt
     8581 ctgggactgg aagtggatta tattcaggat gtgatcgatg ctcacagcga tttgtacgat
     8641 acccctgatt tcgattacaa aatagggtcg gtgcattatc tggacccgat gcccgaaggt
     8701 ggttttttca gcgtggatgg caagttgtat aacttccaaa aaaagatgaa tgaaatttat
     8761 cggggtgata ttcggatggt tgtcgaacgg ttttttgaaa taagctcaaa tatggttcgc
     8821 aaaggtggat ttgatattgt ggggcatatg gataaaattt cgcaaaacgg aatgaattgc
     8881 aagggttttg acattactca gccctggtac cgaaagttgg caaccgattt gctgcaactc
     8941 attaaagaca agggtttgat gctcgaaatc aataccaaat cctttgcaga actcgggatt
     9001 acataccccg atgtacagtt ttttccggtt atccgggaaa tgggaattcc tatactggta
     9061 aattccgatt gtcattatcc cgataaaata ctggacggat ttggtccggt ttacgagttg
     9121 ttgatacagg caggatttca atcgaagatg gaactgaccg aaaaagggtg ggtggaaagc
     9181 cccctaaccc cctaaagggg ggacttgtag attaacaaaa cttaagcata caataaagac
     9241 ttttaaaata atatttctaa atttctcaaa cactctcaga cgctctaact ccccctttag
     9301 gggggtgggg ggcagggtta gggggccgct ctcattttta tgaatttcaa attaatctct
     9361 ccatatcgac ctacaggcga ccagcccgaa gctatcagac aactggtgga cgggattaat
     9421 atgggtgtgc cctatcagac gttgctgggt gttaccggtt cggggaagac tttcagtgtg
     9481 gctaatgtga ttgagcaggt tcagaaacct acccttgtac tgagccacaa taaaaccctg
     9541 gctgcgcaac tttacagcga attcaagagt tttttcccgg aaaatgccgt ggaatatttc
     9601 gtttcgtatt acgattatta tcagcccgaa gcctacctgc ctaccaccga cgtttatatc
     9661 gaaaaagacc tttccatcaa tcaggaactc gaaaaactcc gtctggccac tacctcggcg
     9721 ctgttgtcgg gcaggagaga tgtattggtg gtttcgtctg tttcgtgtct gtacggtatc
     9781 ggaaatcccg atgattttac cgaaaatgtg attgaaattc gccgcggaca gaaaatcgtt
     9841 agaaatgtat ttttgcgctc gctggtggat agtctgtaca cccgaaacga aatagagctg
     9901 aaccgcggaa acttccgcgt aaagggagat acagtggata tttttctggc ttacaccgat
     9961 tttattctgc gtgtggtgtt ttggggtgat gaaatagacg aaatcctgac ggtcgaaacc
    10021 attgattatc atgtgattga gagttttgaa agctataaaa tctatccggc cagcatattt
    10081 gtgaccacca aaccgcgtat catgaatgca attcaaatga ttgaaagcga tctggaagta
    10141 caggtgggaa attttcgtgc tgccgggaaa gattatgaag caaaacggct ttacgagcgc
    10201 gtaacctacg acctggaaat gattaaggag ctcggctatt gttc
//
LOCUS       DAOPZK010000069        10240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_7626_length_10240_cov_21.207658, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000069 DAOPZK010000000
VERSION     DAOPZK010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7626_length_10240_cov_21.207658"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKH68_05585"
     CDS             complement(<1..732)
                     /locus_tag="PKH68_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNX12443.1"
                     /translation="MVKLKYLFTSLFALAFVISANSQGCVSGGGDVVGVHGFIQSEYN
                     YYMNGTDVNGKSLNQSTFNINRARLGVVGSIPYDIDYYVMIEYSPFTPQNKVHLLDGY
                     VSYTRFSKWAKISVGQFKSPFSLEQNTACSGLYTVNRSEVVSQLAGPQRDLGLVINGG
                     SDTTLVQYSVGMMNGSGMNVVDDNRFKDVVGRVVFNPINNLKIGGSFKYGKINPTDNT
                     KPLNEILRYGVELQYKLQNFLLQSEY"
     gene            complement(764..>1231)
                     /locus_tag="PKH68_05590"
     CDS             complement(764..>1231)
                     /locus_tag="PKH68_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX12444.1"
                     /translation="TIKTPVTFSLPTFTATATAGADILKERVKAALDKGFQGSKVSDV
                     LGTPANYFISNYWTEADVNTYGHIKGAYRLNETLKLTDAVGFKNLDPNAVIVPYCWTG
                     QTSAMVSAYLTVLGYNAKGLMFGANNMIQDQLFANNKNSWNKEKPTTELPTVK"
     assembly_gap    1232..1331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1332..1528)
                     /locus_tag="PKH68_05595"
     CDS             complement(<1332..1528)
                     /locus_tag="PKH68_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12445.1"
                     /translation="MKTRNLFKIFLIIAVSALSLSGCIKKEDEPQVDYYKVMTEYMAA
                     NTMDLPDVIASGVVTASAVNA"
     gene            complement(1575..2057)
                     /locus_tag="PKH68_05600"
     CDS             complement(1575..2057)
                     /locus_tag="PKH68_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNX12446.1"
                     /translation="MRTYIKNIFILCIILLTASCRGIYEDATEMAADYKSVVKSITVA
                     DLQAKTDKGESFLLIDVRQPSEYLIDNIPGSVSIPRGLLEFSIGSEAFWSTQYMYPPE
                     KNTEIIVYCKAGSRGILAAVSLMQLGYTNVKNLEGGYDAFNPHQDPNAKPKSSGSCGG
                     "
     gene            complement(2072..3286)
                     /locus_tag="PKH68_05605"
     CDS             complement(2072..3286)
                     /locus_tag="PKH68_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNX12447.1"
                     /translation="MSESKEKNPLVQLFGTFFGKHWPVWVGGIVLGLLNILLFAIKSP
                     WGASGGITNWGDNFYSLFGLFDAKAVTAINESNYGMLVLLIVLGSFAGALFSKEFALR
                     VPPKGEMIKGFLGGTLMAIGATVGIGCSIGAFFSGVPALSGGAIIFAIGMFAGVILSL
                     KYLIWEMEKFPKWSSGKSYNFLAAKPNQGNWQIIAGIIVLIAIMIMSYSYAKDNMVMS
                     WFIIIAGLMGLISQRSRFCIVKSFRDPFMSGESEGAVGVIAGLAVSLIGFTAIKVFGI
                     GAIGELSLRAREMTWVFPHFWAKAAVGGFIFGLGMTIAGGCAVGTLWRMGEGQVKLWF
                     SGLAMVLISPLSAKFLVPVVDNILPQSAKFRNYLPDYLGYDGAFALVLGILLLWYWFV
                     KWNERTGRFSAF"
     gene            complement(3298..3528)
                     /locus_tag="PKH68_05610"
     CDS             complement(3298..3528)
                     /locus_tag="PKH68_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNX12448.1"
                     /translation="MEIKMTLDCKGLSCPMPMMKVAKAMKELKSGEALEMFGTDPGTK
                     TDLPNWCTKTGNEFVEMTELEGGVTRMVVKKA"
     assembly_gap    3775..3874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4133..4762)
                     /locus_tag="PKH68_05615"
     CDS             complement(4133..4762)
                     /locus_tag="PKH68_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNX12449.1"
                     /translation="MLSDKSFGQLSENHLELRFSKGDTIIKQGMFSTNVVFLRKGLAK
                     IHLTGPYHEQIVRLVKAPTYLGLPTTFGDKINQYSVTAVTDTDVCYIDVRVFQTILNE
                     NREFSNDILIELSKNELESFRRCANRTQKQIRGNMADVLLEFSEKIFQSDSFTLPISQ
                     SELANLVDTSRESVNRVLNEFDKDGIIKVNGKQIEILNKKSLNLISLNG"
     gene            4969..5535
                     /locus_tag="PKH68_05620"
     CDS             4969..5535
                     /locus_tag="PKH68_05620"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNX12450.1"
                     /translation="MEKSNEVTLEMLQKQVDELTNKVAYLENNRKDQLSMAIVSGDMD
                     KILAAMVISVAAAAMDTQVKLFFSFWSLSALRDPKKSPKGKDFISKMFGIMLPNGKDK
                     LKLSNMNMAGMGPMMIKMLMKKQNVMSLGEMFRQAGELGVEITICEMSMGLMGFKKEE
                     FIDYPHLSYAGAATFVADAGESSIQLFI"
     gene            5684..5935
                     /locus_tag="PKH68_05625"
     CDS             5684..5935
                     /locus_tag="PKH68_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNX12451.1"
                     /translation="MTAEELKSVEVAKSVDARGTACPGPLLEAKKAIGTIKAGDVMEI
                     LSSDEGTKQDIPKWCNKQGHEYLGFAEENGYFKVYMKKK"
     gene            6039..>6503
                     /locus_tag="PKH68_05630"
     CDS             6039..>6503
                     /locus_tag="PKH68_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNX12452.1"
                     /translation="MTNIEENIQDNTNSNSPSGVRGSSPSGAGGTKKSAKILVFSTEK
                     ISDPAIDMAGLLKLHYPPTVYTISIPCSSGIKPSWILYAYEKGFDGVFIAADGSDCVF
                     GESCTEKTGNLVSKTHNLMKEKELDPARLRMAAICSVCSESFVKQMRNFNDYL"
     assembly_gap    6504..6603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6604..7893
                     /locus_tag="PKH68_05635"
     CDS             <6604..7893
                     /locus_tag="PKH68_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNX12453.1"
                     /translation="IKNISYDALVIGAGIAGGEAALNLANTGYKVLLVDKELSVGGKM
                     IMLSKVFPTLDCSACITTPKVSEISRHPNITIFTGTEVSNIRKLSDNEFTAKVTRQPR
                     YVHIEDCTACQLCEKACPVNVRDQYQFGLIGRKAAFIPFSICSPKAAAIDIENCTLCG
                     ACEKVCPTNCIDFTMETEIMNLHVKTVIIATGFDMFDAQKMPRYGYGQYKNVITALQM
                     ERELAPTRPFNTILRPGDGKVPDKIAYVLCVGSRDASVGNPICSQICCMYSIKQAQLL
                     MGALPMADVTIYYINIRSFGKGFEEFYQQAKGMGVNFVKGKIGRITEKEGGNLILRYE
                     DINEGKLQEAEHDMVILSTGVLPNKGIDKFFENETLALDEFSFVGQSDLLASPSKTSI
                     KGVFVAGTATGPMDIPDSILSAGSASSEAISYLVQHP"
     gene            7907..9847
                     /locus_tag="PKH68_05640"
     CDS             7907..9847
                     /locus_tag="PKH68_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNX12454.1"
                     /translation="MEKKPKKIGVYICHCGGNISDYVDVEKVRQSIEGEDIVFISKTT
                     MFACSDSNQRDMVEDIKANDLDGVIVASCSPKLHQLTFRNVSVRADLNKYTYVHANIR
                     EQASWAHSDNKMGATEKAIHLVKAAIAKSRYAEALETNKIKAKKTVAVIGAGVAGMKA
                     AISLAEKDTEVILIEKEPFVGGRVVQWDTLSTTTETGKDMVKRLYTELTKFDNITIMT
                     SSEVIENKGSVGNISLKVKKTPRYINASACHPDDLKRAIEVCPVEVPDEFNFRTNSRK
                     AIYKNYPSEYPQIPVIDIKNCTRCGECEKVCKAIDFTEKEEIIDITVGSVLMATGFDP
                     YTPAYGEFGYATSDNVITLPQFKRMIDTQFDKLVHNGREVKNIAYIYCVGSRQTDGEN
                     TYCSRNCCTSTIHAAVTAKKKFNNITNYHFNRGLRTYGKQEILYADSLRQGDIYLQSY
                     EDGLPTVTTEARKTIVKINDILTNGREIEVEADLVVLVTGMVPRSDNNTIGSLFKLPK
                     GRDKFYNEVHMKLRPVETVIDGITIAGACQGPKNIAESVNSALSAATKSYSIVSKGEL
                     ETDPLVATIDPDKCTWCGACEEACPFDAILKIEKDKKVIAEINNTVCKGCGMCAPVCE
                     PDAINLIACSSAEIMSMIDALA"
     gene            9908..>10240
                     /locus_tag="PKH68_05645"
     CDS             9908..>10240
                     /locus_tag="PKH68_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNX12455.1"
                     /translation="MKEIENDIQDKANSNPPSGAGGGIEKGKTAKYLREKLGGIPEKA
                     KENLKVYNGIKKQILDALKDQDLTVMQLTEKLNMPSDEVVYYLMSLVKYGFVKTGDID
                     DMDEYYTYK"
BASE COUNT         3052 a         2141 c         2065 g         2682 t
ORIGIN      
        1 gtattcagac tgaagcagga agttttgcag tttgtactgt agttctactc cgtatcgcaa
       61 aatttcattc agcggttttg tgttgtcggt tggatttatt ttgccgtatt tgaaacttcc
      121 gcctattttg agattgttta ttgggttgaa aaccacacgg cccaccacat ctttgaaccg
      181 attatcgtcc accacattca tacccgatcc gttcatcatt cccacgctgt actgaaccaa
      241 agtggtatcg ctgccgccgt tgattaccag gcccaaatcc cgctgaggac ctgcaagctg
      301 gctgacaact tccgaacggt taacggtata aagtcccgag caagctgtgt tttgttccag
      361 actgaatgga gatttgaact gaccaacaga tatttttgcc catttgctga aacgggtgta
      421 agatacatat ccatccagca aatgcacttt gttttgtggt gtaaacggac tgtactctat
      481 cattacataa taatctatat cgtaaggaat ggatccaacc acacccagtc gtgctctgtt
      541 gatgttgaat gtgctttgat tcagactttt tccgttgaca tctgttccgt tcatgtaata
      601 gttgtattca gactggataa atccgtgaac acctaccacg tcaccacctc ccgatacgca
      661 accctgcgaa tttgccgaaa tgacaaatgc aagtgcaaaa agggatgtga ataaatattt
      721 taattttacc atgatataaa tattataaaa tgtatgataa ctattatttc acagtgggta
      781 attcagttgt tggtttttcc ttgttccagc tgtttttgtt gtttgcaaac aattgatcct
      841 ggatcatatt attagcccca aacataagtc ctttggcatt gtaacccaat acggtaagat
      901 aagcagacac catggccgaa gtttgtcctg tccagcaata aggtacaatt actgcatttg
      961 gatccaggtt tttgaatcct acagcatccg tcagtttcaa tgtctcgttc aatctgtatg
     1021 cgcctttgat atgtccgtag gtgttcacat cggcttcagt ccaatagttt gaaatgaagt
     1081 aatttgcagg tgttcccaat acatctgaaa ctttcgaacc ctgaaatcct ttgtcaagtg
     1141 cagcttttac tctttctttc aaaatatcgg cacctgcagt ggcggtagca gtaaaagtgg
     1201 gaaggctgaa agttacagga gttttgatgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn ntttgcattt acagcactgg cagtaaccac tccactggca ataacatcgg
     1381 gcaaatccat ggtgttggct gccatatact ctgtcattac tttgtagtaa tctacctgtg
     1441 gttcgtcttc ttttttaata caacctgaaa gactgagtgc tgaaacagcg ataatcagaa
     1501 agattttgaa taaattacgt gttttcataa gatttaattg tttaattttt atttaaaaga
     1561 atttattgtt tatgtcaccc accgcaacta cccgatgatt ttggtttcgc attcgggtcc
     1621 tgatgggggt tgaaggcatc gtatccgcct tccagatttt tcacattggt gtatccaagc
     1681 tgcatcagcg atacagcggc taaaattccc cgacttccgg ctttacagta aacaataatt
     1741 tctgtgtttt tttcgggcgg gtacatgtat tgtgttgacc agaatgcttc cgaaccaatg
     1801 ctaaactcga gcaatccgcg cggaatggaa accgaaccgg gaatgttatc gatcaggtac
     1861 tcactcggtt ggcgcacatc aataagcaag aaactttcac ctttgtcggt ttttgcctgg
     1921 agatcggcta ctgttatcga tttcactacc gatttgtaat cggcagccat ttcggtggca
     1981 tcttcatata ttcctctgca acttgctgtg agtaatataa tgcaaaggat aaagatattt
     2041 tttatgtaag ttctcatact taatatattt tttagaatgc actgaagcga ccagttctct
     2101 cattccattt cacaaaccag taccacaata ataaaatgcc gagtacaagt gcaaatgcac
     2161 catcgtaacc caggtagtcg ggcaggtaat ttctgaattt ggcactttga ggcaaaatat
     2221 tatcaactac cggcaccaga aatttggcag ataacggtga aataagcacc atcgccaatc
     2281 ctgagaacca cagtttcacc tgaccttcgc ccatgcgcca aagtgtacct acggcacaac
     2341 cgcctgctat ggtcattccc agaccgaaaa tgaaacctcc aacagccgcc ttagcccaga
     2401 aatgcgggaa tacccacgtc atttcgcggg cacgaagcga aagttcgccg atagcaccga
     2461 taccaaatac cttgatagcc gtgaatccaa taagacttac tgccagcccg gctataacgc
     2521 ccacagctcc ttccgactcg ccgctcataa acggatcgcg gaatgatttt acaatgcaaa
     2581 atctggatcg ttgggagata agtcccatca agcctgcaat aataataaac cacgacatga
     2641 ccatattatc tttggcgtac gagtagctca ttatcataat ggcaattaac acgatgattc
     2701 cggctataat ctgccagttg ccctgattgg gcttggctgc cagaaagttg taactttttc
     2761 cggaactcca tttggggaat ttttccattt cccaaatcag gtatttcagc gatagaataa
     2821 cgccggcaaa cattccgatg gcaaaaatga tagcgccacc cgaaagtgcc ggtacgccgc
     2881 tgaaaaacgc accaatcgag cagccaattc ccaccgtggc acctattgcc atcaatgttc
     2941 cacccagaaa tcctttaatc atttcacctt tggggggaac acgcagagca aattctttcg
     3001 aaaataaagc gcctgcaaat gagcccaata cgataagcag taccagcatt ccgtagttgc
     3061 tttcgttgat ggcagtaact gctttggcat caaataaacc aaaaagcgag taaaaattat
     3121 cgccccagtt ggtaatgccg ccgctggctc cccacggact tttgatggca aacaataaaa
     3181 tattgagcaa gcccaatacg ataccgccta cccaaaccgg ccagtgcttt ccgaaaaatg
     3241 ttccgaaaag ctgtaccaat ggattttttt cttttgattc ggacatacgc ggctctccta
     3301 tgcttttttg actaccatgc gtgttacgcc accttcgagt tcggtcattt ccacaaactc
     3361 gtttccggtt tttgtacacc agttgggcag atcggttttt gtgcccggat cggtaccgaa
     3421 catctccagc gcttcgcccg atttcagttc tttcattgct tttgctactt tcatcatagg
     3481 catcggacaa gatagtccct tgcagtctaa tgtcattttg atttccatgt tattttatgt
     3541 ttaaaagtta tgatgcaaag tttcattaaa taattatttc ttcaataaaa aaacagatga
     3601 tgtaattcat catttcgaac ttagttggac agataaaaac atgatttaaa tcaatattta
     3661 tgcagttaaa aatcattttt cagaaaaata attgagcatc cattattctg attaaaaacc
     3721 ggtttctata gtctctaatc gggaaaccta aactgaaata aatgtaaata gtcannnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagctc accggattga cacctgaaag
     3901 tgtaattaga attctctcga aatttaaaaa cgacaaactg atacacgtgg aaggaaaaat
     3961 gataactatt ctggatatgg caaaattgaa acagataagc gattacggat aatcattcca
     4021 ttttttatca ataacaaaaa caggctgtcc gtgcaaagcg aacagcctgt tttttcttta
     4081 gctataagat ctgaataatg ttcagaatta ttaagtgggg ttagggatag ctttagccat
     4141 tcagactaat caggttcaga gattttttgt tcaaaatctc aatctgtttg ccatttactt
     4201 taattattcc gtctttatca aattcgttga gtacgcggtt cacactttcc cgactggtat
     4261 ccaccagatt tgccaattcc gattgcgaaa tgggcagggt aaaactatca gactggaata
     4321 ttttttccga aaattcaagt aatacatcgg ccatattgcc gcgtatttgt ttttgggtac
     4381 gattggcaca acggcggaat gattccaact cgttcttgct aagttcaatc agtatatcgt
     4441 tcgaaaattc cctgttctca ttcagaatgg tctgaaatac acgcacatca atataacaaa
     4501 catcggtgtc ggtaactgct gtaaccgaat attgatttat cttatcgcca aacgtagtgg
     4561 gtaatccaag ataagtcggc gctttaacca gtcgaacaat ctgctcatgg taaggaccgg
     4621 ttaaatgaat tttggccaat cctttacgaa gaaaaaccac atttgttgaa aacattccct
     4681 gttttataat cgtatcacct ttagaaaatc gtagttccag gtgattttca gacaattgcc
     4741 cgaaactttt atcactgagc atcgaagttg cacgtgtatt aaaaacacaa ttcttacagt
     4801 cgttttgcat ggattcttga ttaaagatta cgatacaaat ataataaatg tgatgcatat
     4861 cacaaaataa tgaaacctat tacacaataa ataaatgaat attaatacgg caagtatcta
     4921 tatttgcaga aatatttctt taattaaatt caatcaataa atatagttat ggaaaaatca
     4981 aatgaagtaa ccctggaaat gcttcaaaaa caggtagatg aactaaccaa caaagtagct
     5041 tatctcgaaa acaatcggaa ggatcagttg tcaatggcca ttgtatcagg cgatatggat
     5101 aaaatcctcg cagcgatggt aatttcggtc gcagcagcag ctatggatac acaggtaaaa
     5161 ctgttcttct ccttctggtc actttccgcc ttgcgcgacc cgaaaaaaag tccgaaaggc
     5221 aaagatttta tctccaaaat gtttggcatt atgctcccca acggcaagga taaactcaaa
     5281 ctgtcgaaca tgaatatggc cggaatgggt cccatgatga taaaaatgct gatgaaaaaa
     5341 cagaacgtca tgtcgttagg cgaaatgttc agacaggcag gtgagctggg tgttgaaatc
     5401 accatttgcg aaatgtccat gggcctgatg ggcttcaaaa aggaagaatt cattgattat
     5461 ccacatctga gctatgccgg tgcagctact tttgtggccg acgcaggcga aagttcaata
     5521 caattattta tttaaaagcc ccctaaatcc cccgaagggg gaaatggagc gttttccaac
     5581 agtttattaa aaagtaattt tcaatatata aataacaatg acctttgcac ttcaaattcc
     5641 ccctttaggg ggttaggggg caggtattta aaacattaca atcatgacag cagaagaatt
     5701 aaaatcagta gaagtagcaa aatcagttga tgcacgcggt acagcatgtc cgggaccact
     5761 attggaagcc aaaaaagcaa tcggcaccat caaagccggt gatgttatgg aaatcctatc
     5821 gtccgatgaa ggaacaaaac aggacatccc aaaatggtgc aacaaacaag gacatgagta
     5881 tctgggcttt gccgaagaaa acggatactt caaagtgtac atgaagaaaa aataaaaagt
     5941 tttacgaccc ccaaccccta aaggggagtt agagaaaacc tctaactccc ttttcgggaa
     6001 tagggaattt tggatttgac aatttataaa tacattgtat gacaaatatt gaagaaaata
     6061 tacaagacaa tactaactct aattcccctt caggggttag gggtagctcc ccttcagggg
     6121 ccgggggtac taaaaaaagt gcaaaaattc tggtattttc caccgaaaaa atatccgatc
     6181 cagctattga catggccgga cttctcaaat tacattatcc gccaacggtt tacaccattt
     6241 ccattccatg ttcaagcgga ataaagccga gctggatact ctatgcctac gaaaaaggtt
     6301 tcgacggtgt atttatagcg gccgacggaa gcgactgcgt atttggcgag tcctgcaccg
     6361 aaaaaaccgg caatctggtg tcgaaaactc acaatctgat gaaagaaaaa gaacttgatc
     6421 ctgcccgttt gcgcatggct gccatctgtt cggtttgcag cgaatcgttt gttaagcaaa
     6481 tgcgtaactt caatgattat ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnattaaaa atataagtta cgacgcactg gtgatcggag ccggtatagc cggtggcgaa
     6661 gctgcgctga acctggccaa taccgggtat aaagtgctgc tggtggacaa agaactttcg
     6721 gtgggcggaa aaatgatcat gctcagcaag gtgttcccaa cactcgattg ctcggcctgt
     6781 atcaccactc ctaaagtatc cgaaatttca cgccacccga atatcaccat attcacaggt
     6841 acagaagtga gcaatatccg caaactcagc gataatgaat tcactgcaaa agtaaccagg
     6901 cagccacgtt atgtacatat tgaagattgt accgcctgcc agctttgcga aaaagcctgc
     6961 ccggtcaatg tacgcgacca gtatcagttt ggactgatag gccgcaaagc tgcatttatt
     7021 ccattcagca tttgcagccc aaaagcggct gccatcgata ttgaaaattg cacgctttgt
     7081 ggtgcctgcg aaaaagtttg ccctaccaac tgcattgatt ttacgatgga gactgaaatt
     7141 atgaatttgc atgtgaaaac ggtaattata gctaccggat ttgatatgtt cgatgcccaa
     7201 aaaatgcccc gatacggtta cggacaatat aaaaatgtaa ttactgccct tcaaatggaa
     7261 cgtgaactgg ctccaacccg tccgttcaac accatcctcc gcccgggtga tggaaaagtg
     7321 cccgataaaa tagcctacgt tctatgtgtg ggctcacgcg atgcctcagt gggtaatccc
     7381 atctgttcgc aaatttgttg catgtattcc atcaagcagg cgcagttgct tatgggcgct
     7441 ttgccaatgg ccgatgtaac catttattac atcaatatcc gctcgtttgg taagggattt
     7501 gaagagttct accaacaggc caaagggatg ggcgtgaact ttgtaaaagg aaaaatcgga
     7561 cgcatcaccg aaaaagaagg cggaaacctc attctccgct acgaagatat taatgaggga
     7621 aaacttcagg aagccgaaca tgacatggta attttgtcaa caggagtatt accaaacaag
     7681 ggaattgaca aattcttcga aaacgaaacg cttgcactgg atgaatttag ttttgtagga
     7741 caaagtgatt tgcttgccag cccttccaaa acaagtatca aaggtgtctt tgtagcaggt
     7801 accgccacag gaccgatgga tattcccgat tctatacttt cggccggttc ggcatcgtct
     7861 gaagcaatca gttacttagt tcaacaccct taaatacctg ctaatcatgg aaaagaaacc
     7921 aaagaaaata ggagtttata tttgtcattg cggtggcaat atatccgatt atgtagatgt
     7981 ggaaaaagtc cgccagtcga tagagggtga agatattgtc tttatatcca aaaccaccat
     8041 gtttgcctgc tccgatagca atcagcgcga catggtcgaa gatatcaaag ccaatgacct
     8101 tgatggcgta attgtggctt catgttcacc aaaacttcat cagttaacat tccgcaatgt
     8161 atctgtgcgg gctgatttga acaaatacac ctacgtacat gccaatatcc gcgaacaggc
     8221 ttcgtgggcg cactccgaca acaaaatggg agccaccgaa aaggccattc acctggtaaa
     8281 agctgccatt gccaagtcgc gttatgctga ggcactggaa acaaataaaa ttaaagccaa
     8341 aaaaacagtt gctgtaattg gagccggtgt agccggtatg aaagcagcca tttcgctggc
     8401 tgagaaagat accgaagtta ttctcatcga gaaggagcca ttcgttggcg gacgggtagt
     8461 tcagtgggat acgctctcca ccaccaccga aaccggcaaa gatatggtga aacgactgta
     8521 caccgagctg accaaattcg ataacatcac catcatgacc agttccgaag tgattgagaa
     8581 taagggaagt gtgggaaata tttcactcaa agtaaagaaa accccgcgat atatcaatgc
     8641 atcggcatgc catccggatg atctgaaaag agctatcgaa gtttgcccgg ttgaagtgcc
     8701 cgatgagttt aatttcagaa ccaattcacg caaggctatt tacaaaaact acccgagcga
     8761 atatccgcaa attccggtta tcgacattaa aaactgcacc cgatgcggcg aatgcgaaaa
     8821 agtatgtaaa gccattgatt tcactgaaaa agaggaaatt atcgatatta cggtcggttc
     8881 agttctgatg gccaccggtt tcgatcccta cacgcccgct tacggcgaat ttggttatgc
     8941 tacttccgac aatgtgatta cgctgcctca gttcaaacgg atgattgata cgcagtttga
     9001 caagctggtg cacaatggca gggaagttaa aaacatagcc tatatttatt gtgtgggaag
     9061 tcgtcagaca gatggtgaaa atacctattg ttcgcgcaat tgctgtacct ctaccattca
     9121 tgccgctgta actgccaaaa agaaattcaa taatattacc aattatcact tcaaccgcgg
     9181 tctgcgcacg tatggaaaac aggaaatcct gtacgccgat tcgctgcgtc agggcgatat
     9241 ttacctgcaa tcgtacgaag acggtctgcc aactgtaact accgaagctc gtaaaaccat
     9301 tgtaaaaatc aatgatattc tgaccaacgg cagagaaata gaagtggaag ccgatttggt
     9361 ggtattggtt accggaatgg tcccacgcag cgacaacaat acgattggaa gtctgttcaa
     9421 attacccaaa ggacgcgata aattctacaa cgaggtacac atgaaacttc gtccggtgga
     9481 aacggttatc gatggaatta ccattgccgg agcgtgtcag ggaccgaaaa acattgcaga
     9541 atcggttaat tcagcacttt cggctgctac caaatcgtat tccatagtaa gtaaaggcga
     9601 actggaaacc gatccgctgg tagcaaccat tgatcccgac aagtgtacct ggtgcggtgc
     9661 ctgcgaagaa gcttgtccct ttgatgctat tctgaaaata gaaaaagaca aaaaagtgat
     9721 agccgaaatc aataataccg tatgtaaagg ttgcggcatg tgtgctccgg tttgtgagcc
     9781 cgatgcaata aacctcattg catgttcgag tgccgaaata atgagtatga ttgatgcgct
     9841 ggcgtaaacc cctaacccct gaatgggaat aagaaatatt agttttgaat attacaaaat
     9901 atttgatatg aaagaaatag aaaacgacat acaagataaa gctaactcca accccccttc
     9961 aggggccggg ggtggtatag aaaaaggaaa aactgctaaa tacctgcgcg aaaaactggg
    10021 aggaattccg gagaaggcga aagaaaatct gaaggtttac aacggcatca aaaagcaaat
    10081 actggacgcc cttaaagatc aggacctcac agtgatgcag ctgaccgaaa aactgaatat
    10141 gccttccgac gaagtggtat attatttaat gtcgctggtg aaatatggtt ttgtgaaaac
    10201 cggcgacatc gatgatatgg acgagtatta cacgtataaa
//
LOCUS       DAOPZK010000070        10237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_7629_length_10237_cov_20.475938, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000070 DAOPZK010000000
VERSION     DAOPZK010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7629_length_10237_cov_20.475938"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..890
                     /locus_tag="PKH68_05650"
     CDS             3..890
                     /locus_tag="PKH68_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNX12456.1"
                     /translation="MIYLQSHIGELAALFVSVCWTMSALFFEKASKKIGSLPVNIIRL
                     AMAIIFLGLTTLVTRGLFFPTDATRYNWIWLGLSGVIGFFLGDLFLFKSYTVIGARTA
                     ALIMSLAPMLTAVIGWFFLDEILNAKSILAILLSVSGIMIVIANKNLKLNIPFKGFLL
                     AFGGALGQAVGLILSKKGMGDYDPVAATQIRAIFGITCFVVFITLVGRWDKVLSAVRD
                     RQSMKSVTVGSVFGPFIGVALGLFAIQNTKTGIASTLMALVPIFIIIPSSIMFKEKIT
                     ARQVVGAVITIAGASLFFL"
     gene            complement(895..1779)
                     /gene="dapA"
                     /locus_tag="PKH68_05655"
     CDS             complement(895..1779)
                     /gene="dapA"
                     /locus_tag="PKH68_05655"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNX12457.1"
                     /translation="MLNTKLIGMGVALITPFKEDETIDFDALARLVEHQIKNGTDYLV
                     VCGTTAETPTLTEEEKDEIKSFVVNCAAGRIPIVLGVGGNNTKAVANQLQTKDFTGID
                     AILSVTPYYNKPSQEGLYQHYAAIAKASPLPVILYNVPGRTGVNMLPETTIRLANEFT
                     NICAVKEASGNFSQIDEIIKNKPADFLVISGDDGITFPLITLGAVGVISVIGNAFPRE
                     FSKMVRLALQGEYNNARKIHHRFTELFSLLFVEGNPAGVKSMLAVMGMIENKLRLPLV
                     PNTIKTYEKIRKVLNELT"
     gene            complement(1873..2484)
                     /locus_tag="PKH68_05660"
     CDS             complement(1873..2484)
                     /locus_tag="PKH68_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12458.1"
                     /translation="MKKLNQYLFKKRSASYLKSTNRIRRFVNYETANTVLLLFESDYI
                     EKNRYIRKVIGQLVNDGKKVYAWGYINNKKATTAILPSFKIMDKSSVDWLECPKEQLL
                     KELRDESYDLLIDLTLTDHLPLQYVCLYANAAFKTGMSRNIDDILDFKIKINIPENFE
                     TNDQTGDENKTEFEDLNESLFHTDQQFLFEQIIFYLKNIQTTD"
     gene            complement(2576..>4469)
                     /gene="ligA"
                     /locus_tag="PKH68_05665"
     CDS             complement(2576..>4469)
                     /gene="ligA"
                     /locus_tag="PKH68_05665"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNX12459.1"
                     /translation="LLKELQELEAAHPEHFDPNSPTQRIGSDINSSFKQVKHTYPMLS
                     LANTYSEGEVTDFYERVRKGLNDDFELVCELKYDGTSISLTYENGELKQAVTRGDGEK
                     GDDVTANVKTIRSIPLRLQGNNYPPLFEIRGEILMPWAVFDDLNKEREEQEEMLFANP
                     RNAASGTLKLQNSAEVSRRRLDGYFYYLLGDKLPSDLHYNNLQAARSWGFKVSDAIKV
                     CPTLKEVLDFINYWDVERKNLPVATDGIVIKVNSLTQQRNLGFTAKSPRWAIAYKFQA
                     EKAVTRLNSVSYQVGRTGAITPVANLDPVQLSGTVVKRASLHNADIIRSLDLHIGDMV
                     FVEKGGEIIPKITAVDKDARFMIGDKVEFIQKCPECGAQLMRFEGEAAHYCPNDNACP
                     PQIKGKIEHFVSRKAMNIDGLGSETINLLYQNELLHNIADIYELKVSDLSRLERLGMK
                     SAYNIRDGVEKSKQVPFERVVFALGIRFVGETTAKKLAMAFKTIENLEKATAEELVQV
                     DEIGERIAQSVVAYFADERNIRVVERLKSYGLQLSVSEDKLVNKSDILAGKTIVISGT
                     FSTHSRDEYKVIIEQNGGKNTGSVSAKTSFILAGENMGPEKLNKAEKLNVQIMNEEEF
                     LKLIGG"
     assembly_gap    4470..4569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4632..5876)
                     /locus_tag="PKH68_05670"
     CDS             complement(4632..5876)
                     /locus_tag="PKH68_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX12460.1"
                     /translation="MNFEYFIAKRIHFEKKGKKNVSRPAVRIATIAIAVGLAVMIVAV
                     GVVIGFKKEIRNKTIGFGSHIQISNFDTNNTYEMQPIKINDTLMANLEHIPNVKHIQR
                     FVTKPGIIKTGTAFQGIILKGVGTDYSWDFFKSNLLEGTIPELNDSTPRNEALISKSI
                     ADLLGLKLGDSFYTYFLQDQMRVRKLKITGIYSTNFEEYDKMFVLTDIRTAQKLNGWE
                     EDKYGGLEILISDFDKLEEVNSNVYDVCANQFDQNGNGYYIQTIKQINPQIFSWLDLL
                     DMNVWVILLLMLAVAGFNMISGLLILILEKTNMIGIIKSYGATNWSVRKIFLYQSIFL
                     IGKAMIWGNIIGLGICALQYFFHIIPLDPEAYYVSTVPVTFNWLYILLLNAGTFVASV
                     LMLVGPSYLITKILPATIIRYE"
     gene            complement(6004..>6866)
                     /locus_tag="PKH68_05675"
     CDS             complement(6004..>6866)
                     /locus_tag="PKH68_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNX12461.1"
                     /translation="VFKSVPKWFFQLYESFIELFVVVILGVFAGVLIEQYFNKIKRFY
                     PKNQWLAFLYGAILPVCSCGVLPLIDSMKKRTSLKTIITFVIAAPLLNPYIMLIAFTV
                     LGWKYLVLRIVFSFLLSFLAGWLLEVVSNRYKAFSWGEYQACGSDCDFIPSRDPFIKT
                     ALMTRKLVPYILAAGLLGFIFALINPKQFLDTLNFSLEPWSTIVMTVIGIPLYVCNGT
                     DIIFLKPLMQYTDLSMGSAIAFSLSASAVCASSIAMLVKFIGRNLTVALVSIIIVLIF
                     IFSAIINLAI"
     assembly_gap    6867..6966
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6967..7205)
                     /locus_tag="PKH68_05680"
     CDS             complement(<6967..7205)
                     /locus_tag="PKH68_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12462.1"
                     /translation="MKRIAFIFILLAIICFQVGAQIRLEYTTPRQPAYKAGDKIELNI
                     IIKTRPETCTKGMKQVKIYLSGLKRDKLTDWKELS"
     gene            complement(7209..7550)
                     /locus_tag="PKH68_05685"
     CDS             complement(7209..7550)
                     /locus_tag="PKH68_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX12463.1"
                     /translation="MKSKIIKISFFAVLLFAVVFNACEKLSQHYKIKQDKCTSCMECV
                     AVCGYGAIQVDSIFRTTSDDFEYETTIRIDPAKCVGCGECFKRCKYEAIKLVMEDDDY
                     EDGHSGATRLR"
     gene            complement(7563..>10237)
                     /locus_tag="PKH68_05690"
     CDS             complement(7563..>10237)
                     /locus_tag="PKH68_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNX12464.1"
                     /translation="VLNTAEDCSTCSYYASEHSTAGMINTIVIAVLVLLGSIWLYAGQ
                     KKFYILLLGIFISGAVAASYFAAPYFFSQQENVPANCPVIDTKGSENTFTAPGSEFQS
                     VRPEKVDKDTAAILPAASDKFSDTSSDEFKNGNTDEFSNASKDEFKSGGNDEFTSASK
                     DEFKSGSNDEFSSAGNEFATTDAKTGTPPPEEVRKINYTMIYEPLAIFLILALISFLI
                     KYEWFRKTRSFFLIASVIYLGFYRGACPCMISSFQNGILMIFGVPVAWESLLWFLVLI
                     PATYLFGKVWCGWLCHLGALQELIGRSSKFNFLAGRKVQRTLKIILLSVFIIWILQML
                     ITRTNIFCEYDPFKVAFNLFSANWLGWVLLPVMIISSLLIHRPFCRTFCPVGLILGWV
                     SMLPGAKKLDKGSTCVNCKMCCNECAVRAMIYENKTTTLKQEDCIMCGECMTSCKKQD
                     ALHVKRIFRKKNVLPIILMMLLVSQSAMAQWECPSRLGGSLKPIGESNLMWAAEITTS
                     AGFIGDYKIANGMMFAGLDYSINHHTLYVEGGVKNWVRNDSLNVTNYQTGKFGMREAF
                     YRFSTDNHKFTLGLQSTKSDDYYLINERMVGANYKATFGNFNLNLLGGSVLKQAARNG
                     TFCTVGYLINVVPGRERALIGNQFGQTNVSMLSLTYIPRSGGGDEFSSEFSGSGASKS
                     IFKLNEIGGLAYYEFGTGKTVTPFLTGLYTSVDLAGINLKPEILVQAAANNNAVLYNF
                     EASKEVNWSSGQLTKLMVKYYGLKEISEGAIALNSYSNVFAGEVLRLDALELPFLQAG
                     IKHSFPTIKSSIKLQAALQTGKTSGFISDNFNTEPPRMKEFDISFTKNFGKYFLVNVY
                     AGYLEHPTLTVIESQIKYLKDASPWGKVELRFTF"
BASE COUNT         2693 a         2170 c         2187 g         2987 t
ORIGIN      
        1 taatgattta tctccaatcg cacataggcg aattagccgc tcttttcgta tcggtatgct
       61 ggaccatgag tgctctcttt ttcgaaaaag cttccaaaaa aatcggttcg ttgccggtaa
      121 atatcatccg gttggctatg gccattattt ttctgggatt gactacactg gttacccgtg
      181 gactgttttt cccaaccgat gccaccaggt acaactggat ttggctcggg ctttcaggcg
      241 tgatcggttt ttttctgggc gatttgtttt tgttcaaatc ctacacggta atcggtgccc
      301 gtacggctgc actgattatg agtcttgctc ccatgctgac ggctgtaatc ggttggtttt
      361 ttctggatga aatactgaat gctaaaagta ttttagctat tctgttgagt gtttccggta
      421 tcatgattgt tatcgccaat aaaaacctga aactcaatat tccctttaaa ggttttttac
      481 tggcttttgg tggtgcactg ggacaggctg taggattaat tttaagtaaa aaggggatgg
      541 gtgattatga tcctgtggca gccacacaga tcagagcaat ttttggcatc acttgttttg
      601 ttgtgtttat tacccttgtg ggcagatggg ataaagtgtt atcggctgta cgtgacaggc
      661 agagtatgaa atcggtaact gttggctcgg ttttcggtcc gtttatcggg gtcgcattgg
      721 ggttgtttgc aattcagaac accaagaccg gtatcgcttc aacactcatg gcgttggtac
      781 ccatttttat tattattcct tcgtccatta tgtttaaaga aaaaattacg gcccgccagg
      841 ttgtgggagc cgtaatcact atagcaggag caagtctttt cttcctttag ttcatcaggt
      901 gagttcgttc agcactttac gaattttttc atacgttttg atggtgttgg gcaccagcgg
      961 caatcgcagt ttgttttcta tcattcccat tacagcgagc atacttttca caccggcagg
     1021 attgccttca acgaataaaa gactaaacaa ttcggtgaac cggtgatgta tcttgcgcgc
     1081 attgttgtat tcgccctgta gtgccaggcg taccatttta ctgaattcac gcgggaatgc
     1141 attgccaatt actgaaataa cgcctacagc accgagggtg ataaggggga aagttatgcc
     1201 atcatcgccc gatatgacca gaaaatcagc cggtttgttt ttgatgattt catctatttg
     1261 tgagaaattt ccggaagctt cctttacagc gcaaatattg gtaaattcat tggccagacg
     1321 gatggtggtt tcgggcagca tgttgacacc ggtacgtccc ggaacattgt aaagtattac
     1381 cggcaggggt gaagccttgg caatagctgc ataatgctga taaagacctt cctgcgaagg
     1441 tttattgtaa tagggtgtga ccgataatat ggcgtcaatt ccggtgaaat cttttgtctg
     1501 caactgatta gccacagctt tagtgttatt gccacctact ccgagtacta taggaattcg
     1561 tcctgcagca caatttacga caaaactttt tatttcatcc ttttcttctt cggtcagggt
     1621 aggggtttcg gcggtggtac cgcaaacgac caggtaatcg gtgccatttt tgatctgatg
     1681 ttccaccaat cgtgccagcg catcaaaatc aatcgtttca tcctctttga acggcgtaat
     1741 cagtgctacg cccattccta tcagttttgt gttcagcatt cggaaaatat ctcgttaaat
     1801 cttattcctg ttggtaatat atttgcaggc tgcaataaga ttgtagttca aattttttgc
     1861 aaaaatacgg ttttaatccg ttgtttgtat atttttcaga taaaatatta tctgttcgaa
     1921 cagaaattgc tgatctgtat gaaacaaact ttcgttcaga tcttcaaatt cggttttgtt
     1981 ctcgtcgccg gtttggtcgt tggtttcaaa attctcggga atattgattt ttattttgaa
     2041 atccagaata tcgtcgatat ttcggctcat gcccgttttg aaagcggcat tggcatagag
     2101 gcacacatat tgtagcggta aatgatcggt gagcgtgagg tcaatgagca aatcgtacga
     2161 ttcatcgcgc aattctttca acagctgctc tttggggcat tcgagccagt caacggaact
     2221 tttatccatt attttgaagc ttggcaggat ggctgtggtc gcttttttat tgttgatata
     2281 tccccaggcg tacacttttt tcccgtcgtt gacgagctga ccgatcacct ttcggatgta
     2341 tcggtttttt tcaatataat cgctttcaaa tagcaaaagc acagtattgg cagtctcgta
     2401 gttgacaaat cgtctgatcc tatttgtaga tttcagataa ctggctgaac gctttttgaa
     2461 aagatattga tttaatttct tcatgtttcc ggtcttcctg aaacgatatt cgttgttttg
     2521 tttgcaaatt tatgtaaaaa tgttgaatta ctgttgaaat tggaatttct ttttcctacc
     2581 ctccaatcaa tttcaaaaat tcttcttcat tcataatctg tacattgagt ttttcggctt
     2641 tgttgagctt ttcaggtccc atgttttcac cggcaagtat aaaactggtc ttagctgata
     2701 cacttccggt atttttcccg ccattctgct cgatgataac tttgtattca tcccgcgaat
     2761 gtgttgaaaa tgtgccgctt atcacaattg ttttacctgc cagaatatcg cttttattca
     2821 ccagtttatc ttccgaaacg gataattgta atccgtagga tttcaaccgt tcaacgaccc
     2881 ggatattccg ttcatcggca aaataagcca ccacactctg agcaatccgt tcgccgattt
     2941 catccacctg cacaagctcc tctgcggttg ctttttccag attttctatg gttttgaaag
     3001 ccatagccag ttttttggca gtagtttcac ccacaaatcg tattccaagg gcaaatacaa
     3061 cgcgttcaaa cggtacttgt tttgatttct ccacgccatc cctgatgttg taagccgatt
     3121 tcatacccag cctttcgagg cgcgaaaggt cagagacttt taattcgtaa atatcggcaa
     3181 tattatgcag caattcgttc tgataaagca aattgatggt ttcactcccc aatccatcga
     3241 tattcatggc tttgcggctg acaaaatgtt caatttttcc cttgatttgg ggcggacagg
     3301 cattatcatt ggggcagtaa tgggctgctt caccttcgaa cctcatcaat tgggcaccgc
     3361 attcgggaca tttttgaatg aattccactt tatcgccgat catgaatcgg gcatctttgt
     3421 ccactgctgt aatttttgga ataatttctc cacctttttc cacaaaaacc atatcgccaa
     3481 tgtgtagatc gagcgaacgt ataatgtcgg cattgtgaag cgatgcacgt ttcaccactg
     3541 tacccgaaag ttgtaccgga tccagattag ccacgggggt aatggctccg gtacgtccca
     3601 cctggtacga taccgaattg agacgtgtga cagccttttc tgcctgaaat ttataggcaa
     3661 ttgcccagcg gggcgatttg gctgtgaaac ccaggtttcg ctgctgggtg agcgaattga
     3721 ctttaataac aatgccatca gtagctaccg gaagattttt tcgttccaca tcccagtagt
     3781 tgatgaagtc aagtacttct ttcagcgtgg ggcaaacttt aatggcatcc gacaccttaa
     3841 aaccccagct tcgggctgcc tgcaggttgt tgtaatgtaa atcggagggt agcttatcgc
     3901 ccaacagata atagaaatag ccatccagcc gtcgtctgga aacttcggcc gaattttgca
     3961 gtttgagtgt ccccgaagca gcatttcgcg gattggcaaa cagcatttct tcctgttctt
     4021 cgcgttcttt attcaggtca tcaaatacag cccagggcat gagtatttca ccccgtattt
     4081 cgaatagggg aggataattg ttgccttgaa ggcgtagcgg aatacttcgg atggtcttca
     4141 cattggcagt aacatcgtct cctttttcac cgtcaccacg cgttacagct tgtttgagtt
     4201 ctccgttttc atacgtcagc gagatggatg ttccatcgta tttcagttca cagaccagtt
     4261 caaaatcatc attcaatccc ttgcgcaccc gttcataaaa gtcagtaact tccccttcgg
     4321 agtacgtatt tgcaagtgag agcatcgggt aggtgtgttt tacctgtttg aacgaggagt
     4381 tgatgtcgct gccaatccgt tgagtggggg agttggggtc gaagtgctcc ggatgagccg
     4441 cttccagttc ctgaagttct tttaaaagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnc aataactcaa taactgtttt tttttgaacc ccctttaggg gggcaggggg
     4621 gctcaataaa cctattcata ccgaataatg gttgccggta gtatttttgt aatcagataa
     4681 gagggtccca ccagcatcaa taccgatgct acaaaggtgc ctgcattgag aagtagtatg
     4741 tataaccagt taaaggttac gggcacggta ctcacataat aagcctccgg gtccagcgga
     4801 atgatatgga aaaaatactg cagggcacaa attccgagtc cgatgatatt gccccagatc
     4861 atggccttac caatcaggaa aatggattgg taaagaaata tcttacgtac cgaccagttg
     4921 gtggcaccgt atgatttgat aatcccaatc atatttgttt tttccagaat taaaatcagt
     4981 aatcccgaaa tcatgttaaa gcctgccaca gcaagcatca acaggagtat tacccacaca
     5041 ttcatatcga gcagatccaa ccagctgaat atctgcggat tgatttgttt gatggtctgg
     5101 atgtaataac catttccgtt ttgatcgaac tggtttgcgc agacgtcgta cacattactg
     5161 tttacctcct ccagcttgtc gaaatcgctg atgagtatct ccagcccgcc gtatttatcc
     5221 tcttcccagc cattgagttt ctgggctgta cgaatatcgg tgagtacaaa cattttatca
     5281 tactcttcga aattggtgga ataaattccg gtgattttta gtttacgcac acgcatttga
     5341 tcctgcagaa agtaggtata aaagctgtcg ccgagtttca gtccgagcaa atcggctatt
     5401 gattttgaaa taagggcttc gttgcggggt gtgctgtcat tcagttcggg tatggtacct
     5461 tccagcaaat tcgatttgaa aaaatcccaa ctataatcgg tgcctacccc tttaagaata
     5521 ataccctgaa atgctgttcc ggttttgata attccgggtt tggtcacaaa tcgctggatg
     5581 tgtttcacat tggggatatg ctccagatta gccatcagcg tatcatttat tttgattggc
     5641 tgcatttcat aggtgttgtt ggtgtcgaag ttgctgatct ggatatgcga accgaatccg
     5701 atggttttat tacgaatttc ctttttgaaa ccaatcacca cacccacagc cacaatcatc
     5761 accgccaaac ctacggcaat ggcaatggta gctatccgca ctgccggtcg ggagacattt
     5821 ttcttccctt ttttttcgaa atgaatacgt ttggctatga aatattcaaa attcatggaa
     5881 tggagaaatt attaaggcaa tataaattta ccttacaaaa gtacagaaaa agaatttata
     5941 aaaaaacagg aattcaggaa ctactcagta tgtctgttgg aaccgaaaaa aatgctgtga
     6001 aatttaaatt gccagattaa tgatagctga gaaaataaaa ataagtacaa tgataatgga
     6061 aacaagagct accgtgagat ttcggccaat aaacttaaca agcatggcta tggacgatgc
     6121 gcacacagcc gaagccgata gcgaaaaagc aatggcactt cccatgctca gatcggtata
     6181 ttgcatgagc ggtttgagga agataatatc tgtaccgtta catacatata gtggaatgcc
     6241 gataacagtc ataacaatcg tagaccaggg ttcgagcgaa aaattcagtg tgtccagaaa
     6301 ttgtttggga ttaatcaatg caaaaataaa gccaagcaga cctgctgcca gaatatacgg
     6361 aaccagtttt cgggtcatca gtgcggtttt gatgaacggg tcgcgactgg gaatgaaatc
     6421 gcaatcggaa ccacaagcct gatattcgcc ccacgaaaag gctttatacc ggttggatac
     6481 cacctcgagc aaccacccgg ccaggaaaga aagcagaaaa gaaaatacaa tgcgcagtac
     6541 caggtacttc cagcccaata cggtgaaggc tataagcata atatacggat tgagcagcgg
     6601 tgccgctatc acaaacgtga tgatggtttt aagcgatgta cgttttttca tggagtcgat
     6661 cagcggaagc acgccgcagg agcaaaccgg caaaatggca ccgtaaagga aagccagcca
     6721 ttggtttttg ggataaaatc gtttgatttt attgaaatac tgctcgataa gcacacctgc
     6781 aaatacaccc agaattacca ccacaaaaag ttcgataaaa ctttcgtaca attggaagaa
     6841 ccatttagga actgatttga ataccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnttcg aaagttcctt ccagtcggtc agtttatccc tttttagccc gctgaggtat
     7021 attttcacct gcttcatccc ttttgtacag gtttcgggtc tggttttgat gatgatgttc
     7081 agttctattt tgtctcccgc tttataggct ggctggcgag gtgtggtgta ttccagtctg
     7141 atttgggcac cgacctgaaa acatataatt gccagcaaga tgaaaatgaa tgcaatccgc
     7201 ttcatattct atctcaaccg tgttgcaccg gaatgaccat cctcataatc gtcatcttcc
     7261 atgaccagtt ttatggcctc gtacttacat cttttgaagc attcgccaca gccaacacac
     7321 tttgccggat caatccgtat cgtagtttca tattcgaaat catcggatgt agtacggaag
     7381 attgaatcaa cctgaatagc accgtaaccg catactgcca cacattccat gcagctggta
     7441 catttatcct gttttatctt gtaatgttgc gagagttttt cgcaggcatt gaaaaccact
     7501 gcgaaaagca gcactgcaaa aaacgatatt ttaatgattt tacttttcat acactttcat
     7561 ttttagaagg tgaaacgaag ctcaactttt ccccacggcg aagcatcttt caggtattta
     7621 atctgacttt caataaccgt taaagtggga tgttccagat aaccggcata tacatttacc
     7681 agaaagtatt tcccaaaatt tttggtaaat gaaatatcaa actctttcat gcgcggaggc
     7741 tcggtattga aattatcgga aataaatccg gatgtttttc ctgtttgaag agctgcctga
     7801 agttttatcg atgatttaat agttgggaat gaatgtttaa tgcctgcctg taaaaaggga
     7861 agttcgagag cgtcgagcct taacacttca ccggcaaaaa cattgctgta cgagttgagc
     7921 gcgatggcac cctctgaaat ttccttcaat ccgtagtatt tcaccatcag tttggtaagt
     7981 tgtccgctcg accagttgac ttccttactt gcttcgaaat tgtacaacac ggcattgtta
     8041 ttcgcagcag cctgaaccag aatttccggt ttcagattaa ttcctgccag gtcaacgctg
     8101 gtgtacagtc cggtgagaaa aggagtgact gttttacctg ttccaaattc gtagtatgcc
     8161 agtccgccta tttcgttcag tttgaaaatg ctttttgaag cacccgaacc cgaaaactca
     8221 gaactgaact catcaccgcc tccggacctg ggtatatagg taagtgaaag catagacacg
     8281 ttggtttgtc cgaattggtt acctatcagt gccctttcac gaccgggaac cacattgatc
     8341 aggtaaccca ccgtgcagaa agtgccgttt ctggcagctt gtttaagcac cgaaccgccc
     8401 agcaggttca ggttgaaatt accaaatgta gctttatagt ttgctccaac catgcgttca
     8461 ttgatcaggt aataatcgtc gcttttggtg gattgaaggc ccagggtgaa cttatgatta
     8521 tcagtggaga aacggtagaa ggcttcccgc ataccgaatt ttcctgtctg atagttggtt
     8581 acattcagac tgtcgttgcg cacccagttt ttcacaccgc cttccacata gagcgtatgg
     8641 tgattgatgc tgtaatccag tccggcaaac atcattccgt tggcaatttt ataatcaccg
     8701 ataaatcctg ccgaagtagt gatttcagca gcccacatca ggttactttc acctatcggc
     8761 ttgagactgc cacccaggcg gctcggacat tcccactgcg ccattgcact ctgcgacact
     8821 aaaagcatca tcagaattat gggcaataca tttttctttc tgaaaattct ttttacatgc
     8881 agagcatcct gcttcttgca tgaagtcata cattcaccac acataataca gtcttcttgt
     8941 ttgagggttg ttgttttatt ttcataaatc atagcgcgta cggcacattc gttgcagcac
     9001 attttacagt tgacacaggt gctgccttta tcgagttttt tggcaccggg aagcatggaa
     9061 acccagccca gtatcaaacc aacggggcag aaagtacggc agaagggacg gtgaattagc
     9121 agcgaactga taatcatgac aggaagaagt acccagccca gccagttggc tgagaagaga
     9181 ttaaatgcca ctttgaaagg atcgtattcg cagaagatat tggtgcgggt aataagcatc
     9241 tgaagtatcc agatgataaa aacggaaagg agtatgattt taagtgtccg ctgtactttt
     9301 cggcctgcca ggaagttgaa tttggaactg cgtccgatga gttcctgcag cgcaccgagg
     9361 tgacacagcc agccacacca cactttgcca aacaggtatg tagccggtat caataccaaa
     9421 aaccacaaca gactttccca ggctacggga actccgaaaa tcatcagtat gccgttttgg
     9481 aacgatgaaa tcatacacgg acaggctcct cggtaaaatc cgagataaat aactgaagca
     9541 atgaggaaga aagaccgggt tttgcgaaac cattcatatt tgatcagaaa gctaatcaga
     9601 gccagtatga gaaagatggc cagcggctca taaatcatcg tataatttat cttacggact
     9661 tcttcgggtg gaggtgtgcc ggttttagca tcggttgttg caaattcatt gcctgcggaa
     9721 gaaaattcat cgttgctgcc gcttttgaat tcatcctttg aggcactcgt aaattcatca
     9781 ttgcctccgc ttttgaattc atccttagaa gcattagaga attcgtcggt gttcccgttt
     9841 ttaaattcat cggaagaggt atcggaaaat ttatcagaag cagccggtaa aatggcagca
     9901 gtatccttgt caactttttc gggacgcaca gactgaaact cactgccggg cgcagtaaag
     9961 gtattttcag atccttttgt atcaattacc ggacaatttg caggaacatt ttcctgttgg
    10021 gagaaaaaat agggtgctgc gaaataactc gcagctacag cgcctgaaat gaaaatgccc
    10081 agcagaagta tgtagaattt cttttgaccg gcatagagcc agatacttcc cagcagcacc
    10141 aatacagcta taacaatggt attgatcata ccggcagttg aatgttcgga ggcataataa
    10201 ctgcaggtgg aacaatcctc ggcggtgttg agtacac
//
LOCUS       DAOPZK010000071         9946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_7961_length_9946_cov_23.132140, whole genome shotgun sequence.
ACCESSION   DAOPZK010000071 DAOPZK010000000
VERSION     DAOPZK010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7961_length_9946_cov_23.132140"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1329
                     /locus_tag="PKH68_05695"
     CDS             160..1329
                     /locus_tag="PKH68_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNX12465.1"
                     /translation="MKRLLTYSVMLCMTALLAAQNYNDTTFNFTVVKENKITSVKNQA
                     NSSTCWSFSGLAFFESELLRMGKPAVDLSEMFVVHHSYTDKAGKYLRMNGTSNFAPGG
                     SFYDDVYAWKTYGMVPDSVMNGLNYGENKHVHGELDALTGAFMTALLKNPNKSISTAW
                     QPAFEGILNAYLGKLPTEFTVNGKKYTPVSYANSLGLNMDDYVSITSFNHHPFYTKFA
                     IEIPDNWRWAESYNIPLDEFGRIFDYAVDNGYTVAWGADVSERGFTRNGIGILPETKA
                     ENLTGTDQAKWIGKQKDMKFDIKGPMIEEKVTQESRQKGFDNYQTTDDHGMQIYGIAK
                     DQNGAQYYMVKNSWGTDNKYKGIWYVSKPFVMSKTINILVHKDAVPKDIRKKMGI"
     gene            complement(1389..1868)
                     /locus_tag="PKH68_05700"
     CDS             complement(1389..1868)
                     /locus_tag="PKH68_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX12466.1"
                     /translation="MLVIRPVEPADNEMLAAIIRQCFHDFKAPTAGTVYEDPTTDRLY
                     DLFHRKNSVLWVAESEGVVVGCCGLYPDAALPDDTVELVKFYIASSARGKGIGKALME
                     KCIASAREFGFRKIYIESLPEFHVAVSLYLKYGFQYIDNALGCSTHPGCNVWMVKEI"
     gene            complement(2047..3150)
                     /gene="ychF"
                     /locus_tag="PKH68_05705"
     CDS             complement(2047..3150)
                     /gene="ychF"
                     /locus_tag="PKH68_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNX12467.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERLNKLAELVNPKKIIPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFDDENVTHVDGSVDPVRDKEIIDAELMIKDLETIEARINKVEKQAKTGGDKDAL
                     RAFHIFTQIRDALMKGNSARTVTFDNKDDAKLAKDLYLLTAKPVMYVCNVDEASAVNG
                     NKYVDALREATKNENAEILIVAAKIESEIAEFETYEERQMFLEEIGLEESGVNRLIKA
                     AYHLLQLETFLTAGPDEVRAWTYHKGWKAPQCAGVIHTDFEKGFIRAEVIKYSDFIAL
                     KSEAACKEAGKMNVEGKEYVVQDGDIMHFRFNV"
     gene            complement(3248..3814)
                     /locus_tag="PKH68_05710"
     CDS             complement(3248..3814)
                     /locus_tag="PKH68_05710"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNX12468.1"
                     /translation="MTKNIAIFASGSGSNAENIIRYFAGSKVLNFPVILSNKPDAFIH
                     ERAKALNVPSLTFSKPDFESGEKILEVLETFQIDAIVLAGFLLKVPAIILKSYPGKII
                     NIHPALLPRHGGKGMYGERVHKAVKEQGDTESGITIHYVNENYDEGNVIFQAVCPVLP
                     TDTCEDIAAKVHALEYEHFPRVIEQIWG"
     gene            3965..4201
                     /locus_tag="PKH68_05715"
     CDS             3965..4201
                     /locus_tag="PKH68_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNX12469.1"
                     /translation="MSEVAEKVKAIIVDKLGVDEADVVETASFTNDLGADSLDTVELI
                     MEFEKEFGVSIPDEEAQKITTVGDAIAHVEALKK"
     gene            4226..5488
                     /gene="fabF"
                     /locus_tag="PKH68_05720"
     CDS             4226..5488
                     /gene="fabF"
                     /locus_tag="PKH68_05720"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNX12470.1"
                     /translation="MELKRVVVTGVGAVTPLGLTAAETWQNVLSGKSGAAPITYFDTS
                     KHKTKFACEVKGFDPLKYFDRKEARKMDLYTQYALAATAEAIENSGIDLEAIDKDEVG
                     VVFAAGIGGISTFEQEVGYYYANQEQGPKFNPFFIPKMISDIAAGQISIQYGFHGPNF
                     STASACASSNNALIDAFNLIRLGKANLIFAGGAEAAITPAGVGGFNALTALSTRNDEP
                     EGASRPFSLSRDGFVMGEGAGCIMLEELEHAKARGANILCEIAGTGLSADAYHLTATH
                     PEGLGAILVMKRALAEAGMKPEELDYINVHGTSTPVGDISESKAIKEVFGEHAYKLNI
                     SSTKSMTGHLLGAAGAVEGIISIFAILNNVVPPTINFHEGDEDPEIDYKLNFTFNKAQ
                     QRTVNAALSNTFGFGGHNASVIFKRYDN"
     gene            5478..>6196
                     /gene="rnc"
                     /locus_tag="PKH68_05725"
     CDS             5478..>6196
                     /gene="rnc"
                     /locus_tag="PKH68_05725"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNX12471.1"
                     /translation="MITKLKLNFLNTQRLFSKKRKEPFLSFFRVLGFYPRNIDYYLLA
                     VRHKSISIQGTDGKLLNNERLEFLGDAVLNSIISDILYHRYPDEQEGFLTNARSNIVK
                     RDTLNDLCMQIGLDRLLITIPHLTPDKSNNIYGNALEALIGAIYLDYGYPKCAEFIKG
                     RLFATLDLLMQTAEENENYKSGLLEWAQKNRLETEFRLQTETVNADNQHTFHTRLLIE
                     GINISEGMGANKKESQQNAAA"
     assembly_gap    6197..6296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6447..8249
                     /locus_tag="PKH68_05730"
     CDS             6447..8249
                     /locus_tag="PKH68_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNX12472.1"
                     /translation="MNPKHLSRLIGTQAKKYGEKTVLYDRAELTDSWTAISWNEMSRQ
                     VDIAARSFLEMGVKNQQRVAQFSQNKSENLIVDFALYSLRAAMIPIYPTSSVQQVEFI
                     VNDAEIEYMFVGEQNQYDVAAEVIKTSKTLKKLIVFDKAVKFSVPEKSMYYADFLAIG
                     QKSTKEAEVEKLRDELDEKDIACIMYTSGTTGNPKGVIMTHEMFNEGMRIHTIRLKSM
                     SDQDISLAFLPLTHVFERTWCYFLLFTGATNYINHRPAEIQQTIRDVRPTLMCAVPRF
                     WEKVYIGVQEVLNDYSPLMLGVVTWAIATGKRHNVDRLRIGKKPDIFLSLSYRIADKL
                     VFSKVKTKLGLENANILPVAGARLSDDIALFFRSIGVPITYGYGLTESTATVTCYDEQ
                     YQIGTVGSIMPDVQVKTAAENEILLKGKTITPGYYNNPEANKKAFTEDGWFKTGDAGT
                     IENNMIILTERLKDLFKTSNGKYIAPQEIETRLGTDKFIEQVAVIGDERNYVTAIIAP
                     SIPALEAYAKEKNITYKDKDELLVLPEIYAFMAERIAAAQKGMASYEMIKKFTLIKIP
                     FTIETGELTNTLKIRRAVVQQKYKAQIEAMYKTP"
     gene            8523..8819
                     /locus_tag="PKH68_05735"
     CDS             8523..8819
                     /locus_tag="PKH68_05735"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNX12473.1"
                     /translation="MERIFAKSTLKNFWETHPDTEQYLKTWYDTAMNSNWKTPNEVKQ
                     TYINASILKDSRIVFNIKGNSYRLVARFNYEKQWIFIRFIGFHDEYDRINANTI"
     gene            8831..9199
                     /locus_tag="PKH68_05740"
     CDS             8831..9199
                     /locus_tag="PKH68_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12474.1"
                     /translation="MEIKPIKTESDYIRALKRLEIIFNAEIGTAESDEADILGLLIDE
                     YEKKQYPIEAPDPIEAIKIRMEEMQLKQIDLVNEIGGKSRVSEILNKKRKLTVEMIRK
                     LTTRLNLSPELLINDYQLTR"
     gene            9328..>9946
                     /locus_tag="PKH68_05745"
     CDS             9328..>9946
                     /locus_tag="PKH68_05745"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNX12475.1"
                     /translation="MVSYKELGLVNSKELFRKAIEGKYAIPAFNFNNMEQLQAIVSAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYAIPIVLHLDHGDSFELCK
                     SCIDYGFSSVMIDGSHLPYEENIALTKKVVEYAHAHDVTVEGELGVLAGVEDEVSAEH
                     HTYTEPDEVVDFVTRTGVDSLAISIGTSHGANKFTPEQCTRDENGR"
BASE COUNT         3080 a         2141 c         2045 g         2580 t
ORIGIN      
        1 aagtcaatat cccgacagta caaaaattct gggattttct gcttcacacg ctggccgcta
       61 ttccgaaaaa ataatcaact caaaaaatcc ttcaggcttc tgacgcataa atatatgtga
      121 cagaagcctg atattgtatt ttaaaattca tatacaataa tgaaacgact acttacttat
      181 tctgtaatgc tttgtatgac agcattactg gcagctcaaa actataacga tactactttt
      241 aatttcacag tggtaaaaga aaataaaatc acctcggtaa aaaatcaggc aaactccagc
      301 acctgctggt cattttcggg actggcattt ttcgaatcgg aattgcttcg aatgggtaaa
      361 cccgctgtag atttatccga aatgtttgtg gttcatcact cctacaccga caaagccggg
      421 aaatacctgc gtatgaacgg caccagcaat tttgcgcccg gcgggtcgtt ttacgacgat
      481 gtatatgcct ggaaaaccta cggaatggtg cccgactcgg taatgaacgg actgaattac
      541 ggtgaaaaca aacatgttca cggtgaactg gatgcgctta ccggtgcatt tatgactgca
      601 ttgctcaaaa atcccaataa atcaatttct accgcctggc agccggcttt cgaaggaatt
      661 ctgaatgcct atctgggaaa attaccgaca gaatttacgg ttaacggaaa gaaatacact
      721 ccggttagtt atgcaaattc attgggacta aatatggacg actacgtttc aattacatca
      781 ttcaaccatc atccatttta cacaaaattc gccatcgaaa tacccgacaa ctggcgctgg
      841 gctgagtctt ataatattcc gctcgatgaa tttggtcgta ttttcgacta tgctgttgat
      901 aatggctata ccgttgcctg gggagccgat gtaagcgaaa ggggatttac ccgaaacggc
      961 attggaatac ttcccgaaac aaaagcagag aaccttaccg gtaccgatca ggcaaaatgg
     1021 attggaaaac aaaaggatat gaagttcgac atcaaaggtc cgatgattga agaaaaagta
     1081 actcaggaaa gccgtcaaaa aggttttgac aactaccaga ccaccgatga ccacggcatg
     1141 cagatatacg gcatagccaa agatcaaaac ggtgcacaat attatatggt gaaaaattcg
     1201 tggggaaccg acaacaagta caaaggcatt tggtatgtaa gcaaaccttt cgtgatgagc
     1261 aaaaccatca atattctggt tcataaggat gccgtgccaa aggatattcg gaaaaaaatg
     1321 ggaatataaa caagtcaata aaaaatccaa acaggcatca cagaaaaact ctgtggtgcc
     1381 tgttttcatt atatctcttt taccatccag acattacatc ccggatgtgt ggagcaacct
     1441 agtgcattgt caatgtattg aaatccatat ttcaggtaaa gtgaaacggc aacgtgaaac
     1501 tccggaagac tttcgatata gattttccgg aaaccaaact ccctggcaga agcaatgcat
     1561 ttttccatca gagcttttcc aattcctttc ccacgggctg acgatgcaat ataaaacttg
     1621 acaagttcca ccgtatcatc cggtagagca gcatcgggat acagtccgca acagccgacc
     1681 actacgccct cactttcagc cacccaaaga acagaattct ttcggtggaa caaatcatac
     1741 aacctatcgg tagtgggatc ttcatacact gtaccggcag tgggtgcttt gaaatcgtga
     1801 aaacactgac gaatgatcgc agcaagcatt tcattgtcgg cgggttccac aggtctgatt
     1861 acaagcataa aggaaaatta ttataaaaaa aactaaccat taaaacttcg aaaactgctt
     1921 gtattaaacc aaattccatt aatcgtatcc ctcttaaagc ctccccttcg gggaggtttg
     1981 gtggggcttt atcttcgggg aggtttagtg gggcttcttc cttttagagg tttagtgggg
     2041 ctacttttac acattaaatc tgaaatgcat gatatcgcca tcctgaacga catattcctt
     2101 gccttccaca ttcattttac ctgcttcctt gcaggctgct tccgatttca gcgcaataaa
     2161 atcactgtat ttaatcactt cggcacggat aaaacctttc tcaaagtcgg tgtggatgac
     2221 accggcacat tgaggtgctt tccaaccttt gtgatacgtc caggcacgaa cttcatccgg
     2281 accggcagtg aggaatgttt ccagctgaag cagatggtag gcagctttaa tcaggcggtt
     2341 tactcccgat tcctccagtc cgatttcttc cagaaacatt tgccgctctt cataggtttc
     2401 aaattcggca atttccgatt caatctttgc agcgacaatg agaatttcag cattttcgtt
     2461 ttttgtagct tcgcgcaggg catcaacgta tttatttcca ttcaccgcac tggcttcatc
     2521 cacattgcac acatacatta ccggttttgc cgtcagcagg tataagtctt tggccagttt
     2581 agcatcgtct ttattgtcga aagttactgt tcgtgccgaa ttgcctttca tcagggcatc
     2641 acgtatctga gtaaaaatat gaaaagcccg cagggcatct ttatctcccc ctgttttggc
     2701 ttgcttttcc actttgttga tgcgggcttc gatggtttcg aggtctttta tcatcagttc
     2761 ggcatcaata atttccttgt cgcgtaccgg atccaccgaa ccatcgacgt gcgtcacgtt
     2821 ttcatcgtcg aagcagcgga gcacatgcag gattgcgtcc gtttcgcgga tattggccag
     2881 aaatttattt cccagccctt cgcctttact tgctcctttc accagtccgg caatatccac
     2941 tatttctact gtggtgggaa tgattttttt gggatttacc agttctgcca gtttattcag
     3001 ccgctcgtcg ggcacagtga ttacgcccac attgggttca atggtgcaaa agggaaaatt
     3061 cgccgcctgt gctttggcat tggaaagaca attgaaaagg gttgatttcc cgacattggg
     3121 aaggccaact ataccgcatt gtaatgacat tttttttgat ttttgctcgc tttgcctaca
     3181 gcgtttctac ttttttagca aaacgaaaat tagtacattc agggagcaaa gatacgacaa
     3241 aaacaggcta tccccaaatc tgctcaatca cccgggggaa atgttcatat tccagtgcat
     3301 gcactttggc ggctatatcc tcgcaggtat cggtgggcaa aaccggacat acggcctgaa
     3361 aaatcacatt tccttcatcg taattttcat tgacataatg gatggtaata ccactttcgg
     3421 tatcgccctg ttcttttacc gctttgtgta cccgttcccc gtacattcct ttgcctccgt
     3481 gccggggaag cagcgcggga tggatattga tgatttttcc gggataagat ttcagtatta
     3541 tagccggcac tttaagcaaa aagcccgcca gcacgatggc gtcaatctga aaggtctcaa
     3601 gcacttcaag tattttttca ccactttcaa aatccggttt cgaaaaggta agcgacggaa
     3661 cattcagtgc tttggcacgt tcgtggataa aggcatccgg cttgttggag agaatgaccg
     3721 gaaaattcaa caccttagaa ccggcaaaat aacggatgat attttcggca ttagagcctg
     3781 aacccgaagc gaaaattgct atgttttttg tcatatccgg atgagttttt tgctcatttc
     3841 acttaaaaat gtgcaaaaac gtgcattttt aagaaaaaat gtgcggtggt ttaatgaaat
     3901 ttgctttact ttgcaggcgc aaatttataa aacaaatttt atttaataac taaaaagaaa
     3961 aattatgtca gaagttgcag aaaaagtaaa agctattatt gtagacaaat tgggtgttga
     4021 cgaagcagat gtagtagaaa cagccagttt tacaaatgat cttggtgccg attctttgga
     4081 cacagtagaa ttaatcatgg aattcgaaaa agaattcggt gtttctattc ctgatgaaga
     4141 agctcaaaaa atcaccacag ttggcgatgc aatcgctcac gtagaagctt tgaaaaaata
     4201 aatcaattcc taaaagtaac attttatgga attaaaaaga gttgtagtaa ctggtgtcgg
     4261 tgccgttacg cctttaggac tgactgctgc ggaaacgtgg cagaacgtct tatcaggcaa
     4321 aagcggtgct gctcctatta cttattttga tacttcaaaa cacaaaacca aatttgcctg
     4381 tgaggtaaaa ggttttgatc ccctgaaata tttcgaccgc aaggaagcgc gaaaaatgga
     4441 tctttacacc cagtacgcct tagccgctac cgccgaagcc atcgaaaaca gtggcattga
     4501 cctggaagcg attgataaag acgaagtggg tgtggttttt gcagccggta tcggtggtat
     4561 cagcacgttc gaacaggaag tgggatacta ttatgccaat caggaacaag gtcccaagtt
     4621 taatcctttc tttattccga aaatgatttc ggatattgct gccggacaaa tttctatcca
     4681 gtacggattt cacggaccga atttttcgac cgcatcggcg tgtgcttcgt ccaacaatgc
     4741 ccttattgat gctttcaacc tcatccggtt gggtaaagcc aatctgatat ttgccggagg
     4801 cgctgaagca gccattacac cggccggagt tggtggtttc aatgcactca ccgctttatc
     4861 tacccgcaat gatgaacccg aaggagcttc cagacctttc agtctgagcc gagacgggtt
     4921 tgtaatgggc gaaggagccg gctgtatcat gctcgaagaa ctggaacatg ccaaagcacg
     4981 cggtgctaac attctgtgcg aaatagccgg aacaggctta tcagccgatg cctatcacct
     5041 cactgccacc catcccgaag gactgggagc tatactggtg atgaaaagag cactggccga
     5101 agccggaatg aaacccgaag agcttgatta tatcaatgtt catggcactt ccacaccggt
     5161 aggcgatatt tcggagtcca aagctatcaa ggaagtattt ggtgaacatg cgtacaaact
     5221 gaatatcagt tccacaaaat caatgacagg ccacctgctc ggtgctgccg gtgcagtgga
     5281 aggaattatc tccatattcg caattctgaa taatgtagtt cctcctacta tcaacttcca
     5341 cgaaggagac gaagaccctg aaattgatta taaactgaat ttcactttca acaaagcgca
     5401 acagcgaact gtaaatgcag cactgtcaaa tacattcgga tttggcggtc ataacgcaag
     5461 tgttatattc aaaagatatg ataactaagt tgaaattaaa cttcttaaac acccaaaggc
     5521 tcttttccaa aaaacggaaa gagcctttcc tgtctttttt cagagtgctg ggattttatc
     5581 cccgcaatat cgactattat ctgttggccg ttcgccacaa atccatttcc attcagggaa
     5641 cagatggaaa acttctcaat aatgaacgat tggaattttt gggtgatgcc gtgctgaatt
     5701 caattatatc agacattctt taccaccgat accccgacga acaggaagga tttctgacca
     5761 atgcccgctc caacattgtg aaacgtgaca cattgaatga tctttgcatg cagattggac
     5821 ttgaccggtt actgattact atcccgcatc tcacacccga taaaagcaat aatatctacg
     5881 gcaatgcact cgaagcgctg ataggagcca tatacctcga ttatggctat ccgaaatgtg
     5941 ccgaattcat taaagggaga ctttttgcaa ccttggattt actgatgcag actgccgagg
     6001 agaatgaaaa ttacaaatca ggattgctgg aatgggcaca aaaaaaccgg ctggaaacgg
     6061 aattcagatt gcagactgaa accgtcaacg ccgacaatca gcacactttc cacacccggc
     6121 tactaataga aggaattaac ataagcgaag gaatgggagc taacaagaaa gaatcgcagc
     6181 agaatgcagc agcacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaaa
     6301 tatttttatc agacaacagt gaatattaac aagaacatta atagcattct atgcgttaaa
     6361 actttaaagt tctctgtatt ttttgtagag ataagacaac aaatctcaga tctcaatata
     6421 aaatcacaga cataaaacaa aaacttatga atccaaaaca tttatcacgc ctgataggca
     6481 cacaagccaa aaaatacgga gaaaaaaccg tactatatga ccgtgcagaa cttacagact
     6541 cctggacagc cattagctgg aatgaaatga gcaggcaggt ggacattgct gcccgaagtt
     6601 ttctggaaat gggcgtgaaa aatcaacagc gtgtagctca gttttcacaa aataaatccg
     6661 aaaatctgat tgttgacttt gctttgtact ccctgcgggc ggccatgatt cccatctacc
     6721 ccacttcatc tgttcagcag gtcgaattca ttgtgaatga tgccgaaata gaatacatgt
     6781 ttgtaggaga acagaatcag tacgatgtag ccgccgaagt aattaagact tctaaaacgc
     6841 tgaaaaaact gatagtattc gacaaggcag tgaaattttc tgttccggaa aaatccatgt
     6901 actacgcaga ctttctggct atcggccaaa aatcgacaaa agaagcagag gtggaaaaac
     6961 tgcgtgacga attggatgaa aaagacattg cctgcattat gtacacttcc ggcaccacgg
     7021 gaaatcccaa aggggtaatt atgacccatg agatgttcaa cgaagggatg cgtattcaca
     7081 ccattcggct caaatcgatg agcgatcagg atatttcatt agcatttctt ccacttacac
     7141 atgtttttga acgtacatgg tgttacttcc ttcttttcac cggagctacc aattacatta
     7201 accaccgtcc ggccgaaatt cagcaaacca ttcgcgatgt tcggcctacg ctgatgtgtg
     7261 ccgttccgcg tttctgggaa aaagtatata tcggagttca ggaagtactc aacgactatt
     7321 cgcccctgat gttaggtgtt gttacctggg ctattgctac gggtaaaaga cacaatgtag
     7381 atcgcttgcg tatcggtaaa aaaccggata tattccttag cctgagttac aggatagccg
     7441 acaaacttgt tttttcgaaa gtgaaaacaa aactcggact tgaaaatgcc aacattctgc
     7501 ccgtagccgg cgcaagactt tcggatgata ttgccctgtt tttccgcagc ataggagtac
     7561 caatcactta cggctacggt ctgactgaaa gtacagccac cgttacctgt tatgatgaac
     7621 aatatcagat aggcaccgta ggttctatca tgcccgatgt acaggtaaaa acagcggcag
     7681 aaaatgaaat actgcttaaa ggcaaaacca ttactccggg atattacaac aaccccgaag
     7741 ccaataaaaa agcctttacc gaagatggat ggttcaaaac aggtgatgcg ggaaccatcg
     7801 aaaacaacat gattattctg accgaacggc taaaagattt attcaagact tcgaacggca
     7861 aatacattgc cccgcaggaa attgaaaccc gcctcgggac cgataaattc attgagcagg
     7921 ttgctgtcat tggcgatgaa cgcaactatg tgactgcaat cattgctcct tccattcccg
     7981 ctctggaagc ttatgccaaa gaaaaaaaca tcacgtataa agataaggac gaattgctgg
     8041 tgctgcccga aatttatgca ttcatggctg aaaggattgc tgcagctcaa aaaggaatgg
     8101 catcttacga aatgattaag aagttcaccc tcatcaaaat accattcacc atcgaaaccg
     8161 gcgaactgac caataccctg aagatacgcc gggctgtggt acaacagaaa tacaaggcac
     8221 aaattgaggc gatgtacaag accccctaac tccctaccgt gtcccgtccc gaagcttcgg
     8281 gatatcggga aaggggaaat aggatgggag ctggttagat tgaactgaaa accttcaaaa
     8341 atgaattgaa tggtatatta cgctctcagg ctgagattta cagtgatttt tttactttag
     8401 gaaaaagcat catcctgata ggattatttt ctatttatta aacaacaaac actaaaaaat
     8461 aatcatcacg aaacgttccc gttttgggaa ctatttacta tctttgcatc cttattcata
     8521 ctatggagag aatatttgca aaaagtacgt tgaaaaactt ttgggaaaca catcccgaca
     8581 ccgagcaata cctgaaaacc tggtacgata cagccatgaa ttccaactgg aagacaccaa
     8641 atgaagttaa acagacatac ataaatgcaa gtattttaaa agacagtcga attgtattta
     8701 acatcaaagg taattcctat agactggtag caagattcaa ctatgaaaaa caatggatat
     8761 ttatccgttt tataggattt catgatgaat atgacaggat taatgccaat accatttaaa
     8821 cgttcaagac atggaaataa aaccaataaa aacagaatca gactacatcc gggcattgaa
     8881 aagacttgaa ataatattta atgctgaaat cggaacagcc gaaagcgatg aagcagatat
     8941 acttggacta ttgattgatg aatatgaaaa aaaacagtat ccgattgaag ctcccgatcc
     9001 gattgaagct ataaaaataa gaatggaaga aatgcagctt aaacagatag atctggtcaa
     9061 cgagataggt ggaaaaagta gggtgtcgga aattttgaac aaaaagagga aactgactgt
     9121 tgaaatgatc cgtaagttaa ccaccagatt aaacctttca ccggaattat taatcaatga
     9181 ctaccaactg acaagataaa aataccattg gcgaatactc ccgggtaacc ttaacaatta
     9241 tcaattttct tgctaaggtt acaaaaaagc agtacttttg cagaaaataa gaaattagta
     9301 ttaacaacat tctaaataat aaacaatatg gtaagttaca aagaattagg cttggttaac
     9361 tccaaagagt tattccgcaa agccattgaa ggaaaatatg caattcctgc attcaacttc
     9421 aacaatatgg aacagttaca ggctatcgtt tcagcttgtg tagaaacaaa atcaccggtt
     9481 attcttcagg tttcgagcgg tgcgcgcaaa tatgccaacc agacattgct ccgctacatg
     9541 gctcagggcg ccgtagaata tgctaaagaa ctcggatatg caattcctat cgttcttcac
     9601 ctggaccacg gcgacagctt cgaactttgc aaaagctgca tcgattatgg tttctcctct
     9661 gtgatgatcg acggttcaca cctgccttac gaagaaaaca tcgcactgac taaaaaagtg
     9721 gtggaatatg ctcacgcaca cgacgtaacc gtagaaggtg aacttggcgt actggcaggc
     9781 gtagaagacg aagtatcggc cgaacaccat acctataccg aacctgatga agtggttgat
     9841 ttcgttaccc gcaccggcgt tgactcactg gctatttcca tcggaacatc gcacggagcc
     9901 aacaaattca cacctgaaca atgcacacgc gacgaaaacg gacgac
//
LOCUS       DAOPZK010000072         9867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_8047_length_9867_cov_21.412760, whole genome shotgun sequence.
ACCESSION   DAOPZK010000072 DAOPZK010000000
VERSION     DAOPZK010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8047_length_9867_cov_21.412760"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="sucC"
                     /locus_tag="PKH68_05750"
     CDS             <1..1028
                     /gene="sucC"
                     /locus_tag="PKH68_05750"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNX12476.1"
                     /translation="KLGFPAVVKAQVLTGGRGKAGGVKLVKNKEEAQKAAETILGMDI
                     KGYKVEKILVAEGVKFHSELYVGLTIDRNSKSVILMASKEGGVEIEEVAKDNPDAILK
                     FPIEADLGMPSFLARKMAFQLFDDFSLVKQAADIFQKLYKICFDTDASLVEINPLVIT
                     DDKRLLALDGKMNFDDNALFRQPEIEKLSEPDENELKEIEAKEKGLSYIKLDGNIGYM
                     VNGAGLAMATMDMIKMYGGEPANFLDIGGSSNPQKVIDAMNLLLSDKNVKVVMINIFG
                     GITRCDDVAKGLLEALRQLDVQVPIVVRLSGTNAKEGLEILKGTNLTVVESMSEAAQK
                     AIALTAP"
     gene            1185..2063
                     /gene="sucD"
                     /locus_tag="PKH68_05755"
     CDS             1185..2063
                     /gene="sucD"
                     /locus_tag="PKH68_05755"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009435237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNX12477.1"
                     /translation="MSILVNKNTRLIVQGITGRDGGFHTLKMKAYGTNVVAGVSPGKG
                     GQEVDGIPVFDTVTDAVNATGANTSIIFVPAAVAADSILEASEAGIELVIAITEGIPT
                     LDMVRVMPYLKKNGTKIIGPNCPGLITPEEALVGILPGNIFKKGNIGLISRSGTLTYE
                     MVNQLTTNGLGQSTCVGIGGDPVAGLYFEELLAMFQKDPETDAIVLIGEIGGDAEERA
                     ALFIKNNVSKPVVAFIAGQSAPPGKRMGHAGAIITSGSGSAAEKIAAFENAGIPVARK
                     PQDIPLLVKRQLQYNS"
     gene            2254..3486
                     /locus_tag="PKH68_05760"
     CDS             2254..3486
                     /locus_tag="PKH68_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNX12478.1"
                     /translation="MALNYIWIAFIVIAFFVALLQMIFFGNISIFNEVLQSIFGSAKS
                     GFEISLGLTGILTFWMGILKVAEHGGALKVVSRMVAPFFTRIYPEIPKDHPALSSIFL
                     NMSANMLGLDNAATPMGLQAMKEMQELNPKKDTASNPMIMFLVLNTAGIVLVPMTVMM
                     YRTQLGAVNPADVFLPIIVATLCAFITGLITVSVIQKINLFNRVVMGVLAGLVGFVIG
                     LVYFFSTLSQEQITAYSSFSAAFIILSIIVIFVLMAVKKKVNVYNSFIEGAKEGFQVA
                     VGIIPYLVAMLVAIGMFRASGTLDLLIDGIRYLVALTGVNTDFVEALPTAFMKPLSGS
                     GARGMMIETMTRYGADSFVGRLSCIIQGSTDTTFYILAVYFGSVKVTNTRYAVMAGLF
                     ADFVGITAAILLGYLFFH"
     gene            3730..4155
                     /gene="ybeY"
                     /locus_tag="PKH68_05765"
     CDS             3730..4155
                     /gene="ybeY"
                     /locus_tag="PKH68_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNX12479.1"
                     /translation="MIHYITENIEMPDINQAVINRWIKVIAAHWGRKVGNITYIFCSD
                     ARILEVNKQYLQHDYFTDIITFDYSSGSVIAGDIFISLDTVADNARQLGVLYNDELNR
                     VLIHGILHLCGQDDKTPETQTEMTAKENAALEILQNEIN"
     gene            4360..5169
                     /locus_tag="PKH68_05770"
     CDS             4360..5169
                     /locus_tag="PKH68_05770"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNX12480.1"
                     /translation="MNVFSGKTILLCFFFILFFSFNSKSQDEYRAEIGLQGGANIYTG
                     DVNTVAKPDYFLQNMKNIQPDFGLFFRYRFNPRTALRLGYDYSTVNGSYIYNVNTSQQ
                     LNQTLHIVDMTGEYNFFDLENDPYKRYSKKYSPYIFAGLGVIYIPDAETLFQKKYAPT
                     VPFGIGLKWKMAKRWNLNIQWTNHLLLSDNLEGKIKLDYGELIKDWDLNGNPVWPNDL
                     SPIPTWTKRINPMNNDLLSGLMIGISFDFWKKGCDCNQSDKMKKKTSVFQR"
     gene            5221..5943
                     /locus_tag="PKH68_05775"
     CDS             5221..5943
                     /locus_tag="PKH68_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNX12481.1"
                     /translation="MSFKEQLDLKSLPKHVAIIMDGNGRWAKERGFDRIYGHQHGVTS
                     VRECTEAAAEIGINYLTLYAFSTENWSRSRKEVDALMELLIDTIEKETPTLNKNNVSL
                     MAIGDLSRLPQGAYEKLQRCILQTKNNTGLRLVLALSYSSRWEISNAFRLISHEISEG
                     KIKTDDITEDLISNYLTTKDIPDPDLMIRTSGELRISNFLLWQLAYTELYFTNTHWPD
                     FRKENFFEAIVEFQKRERRFGK"
     gene            5978..8665
                     /locus_tag="PKH68_05780"
     CDS             5978..8665
                     /locus_tag="PKH68_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNX12482.1"
                     /translation="MQYKKILTFCLLLFFSHLISAQTVVPNDSVVTPQIDYNEPKEYQ
                     IADIQVEGADSYDDFVLIGFSGLQVGQKIVIPGDDITSAVKKFWEQSMFSDVKIELTK
                     VEGDKAWLTIYLQLRPKVSEIIYKGLKKAEIEDIEKRIGILKDKQITPNMSDRATKSI
                     KKYLDEKGFSNADIKVYQMDDPAKPGFVKVDIDVDKKLKTRVHKIYISGNDKLDALQI
                     NKAMKKTNDNNIINIFRTKKFVKDLYEKDKIAAIEKYNEFGYRDAYLVSDSVVPFDDK
                     SVDVYMKFNEGEKYYFRDIKWIGNTIYPYETLNQFLGIKKGDVYNLRLLNKRLQEDED
                     AVAKLYSNKGYLFFNIQPIEVAIQNDSIDFEIHMVEGKPATINEVKITGNTRVYENVV
                     RRELRTVPGALYSQDDIMRTLRELAQMGHFDPEKLSKDMMEGGLKPNPENGTVDLTYK
                     LETKSSDQVEFSAGWGAAGIVGSLGLKFSNFAIQNIFKPETYRIVPQGEGQTFSINAR
                     TNGNYYSSFSLSFLEPWLGGKRPNSLSVSLYYTATSGMSDRYLNAYSSMSSMYGGYGG
                     YGGYGGYGGYGSGYGYGSGYGNTSYETEVDKNKYFRTLGASIGYGKRLSWPDDFFTFY
                     GEVALQRYMINNWYQQETNVTNGVFNNIGLNLTIARNSIDNPIFTRKGSNFSLGLQVT
                     PPYSAFNGKDYSNVNMTDAERYKFLEYHKWKFSAKTFTPLMAVNKTPVFMARAEFAYV
                     GHYNKDARTPFGTYMLGGDGMSGYSGYANEYVAMRGYETGALTPYEYRLNSAGQSQAY
                     AQRAYLYNKFTMELRVPISLEQSANIWALGFLEAGNSFNKISDYNPFNLKRSAGVGVR
                     IYLPMFGIMGIDWGYGFDPQVNEQKAHGSQFHFVIGQEL"
     gene            8785..9300
                     /locus_tag="PKH68_05785"
     CDS             8785..9300
                     /locus_tag="PKH68_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX12483.1"
                     /translation="MKKLLLTLCLLAGIGIGTQAQKFASVDMSYIMKNIPAYESANDQ
                     LNQVSKKWQTEVETSMKDVQQMYKNYQTELVFMSDDMKVKREEEIVAKEKEAQELKRK
                     YFGPEGELFKKRESLMKPIQDEVYNAIQEISKDKDFQLVFDKSSAMSAIFISPKLDIS
                     DDVLKKLGYSK"
     gene            9366..>9867
                     /locus_tag="PKH68_05790"
     CDS             9366..>9867
                     /locus_tag="PKH68_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNX12484.1"
                     /translation="MIKKVALFLIALLPLGAMAQNVKLGHVNSQEIISVMPELETIEK
                     TLKELQDQWEKELLKMREEYNTKVKEFVDKQATMPETIKTARQSEIAEIEQRITTFNQ
                     TANADVVKKQQELVNPVLDKVKKAISDVATEGGYTYIFDISTQGVVFQAKTANDITPL
                     VKKKLGV"
BASE COUNT         3092 a         1897 c         2170 g         2708 t
ORIGIN      
        1 aaaaactcgg atttccagcc gtagtaaaag ctcaggtgct cacaggtgga cgcggaaaag
       61 cgggtggagt aaaattagta aaaaacaagg aagaagctca aaaagctgct gaaaccattc
      121 ttggaatgga catcaaaggt tacaaggtag aaaaaatcct tgttgcagaa ggcgtaaaat
      181 tccactctga actctatgta gggcttacta ttgaccgtaa ctccaaatct gtcattctga
      241 tggcaagcaa ggaaggcgga gtagaaattg aggaagtagc aaaagataat ccggatgcca
      301 ttctgaaatt tccgattgaa gccgatttgg gaatgccctc ttttttagcc cgtaaaatgg
      361 cttttcagct tttcgatgac ttttctttag tcaaacaagc cgccgatata tttcaaaaac
      421 tctataaaat ttgctttgat actgacgctt cgttggttga gattaatcca ctggttatta
      481 ctgacgacaa gcgtttgctg gctttggatg gcaaaatgaa ttttgatgat aatgcactgt
      541 ttcgccagcc cgaaatagaa aaactttcag agccggacga aaacgaactg aaggagattg
      601 aagccaaaga gaaaggattg agttacatta agttagatgg taatatcggt tatatggtga
      661 atggtgccgg cttggctatg gcaaccatgg atatgataaa aatgtatgga ggagaacctg
      721 ccaacttcct cgatattggt ggcagctcca atccacagaa agtgattgat gccatgaatc
      781 tccttctatc tgacaaaaac gtaaaagtag tcatgattaa cattttcggg ggcattaccc
      841 gctgcgacga tgtggctaaa ggactgcttg aagcattacg acaattagat gtccaggtac
      901 ccattgtagt acgtctatcg ggcaccaatg ccaaagaagg actggaaata ctgaagggaa
      961 ccaatctcac tgtggtagaa tccatgagtg aagcagcgca gaaagcaatt gcactaacag
     1021 ccccctaacc ctcccgaaac aagttcggga caggctctaa agggggaaag ttgcgttatt
     1081 gtaacttata cttaaatatg agggaagtta atcaataaat atattattat aaacctaatt
     1141 taattcacgt tccctcccct ttggggaggg ttagggaggg gctcatgagc attttagtaa
     1201 ataaaaatac acgcctgata gttcagggaa ttacaggccg ggacggtgga tttcacacct
     1261 tgaaaatgaa agcgtacgga acaaacgtgg tggctggtgt ttcacccggc aaaggggggc
     1321 aggaagtgga tggaattcct gttttcgata ccgttaccga tgctgtgaac gcaaccggtg
     1381 ccaatacatc tatcattttt gtgcctgcag ccgttgcagc cgatagtatt ctggaggcat
     1441 cagaagccgg aatagaactg gtaattgcca ttaccgaagg cattccaacg ctcgacatgg
     1501 tacgcgtgat gccgtatttg aagaaaaacg gaacaaaaat tatcggacca aactgcccgg
     1561 ggcttatcac tcccgaagaa gcactggtgg gcatattgcc aggaaatatt ttcaaaaaag
     1621 gaaatatagg gcttatcagc cgaagcggta cattgactta tgaaatggtc aatcagctga
     1681 ctaccaacgg actgggacaa agtacctgcg taggtatcgg tggtgatccg gtggccggtc
     1741 tttattttga ggaattactg gccatgtttc agaaagatcc cgaaacggat gccatcgtac
     1801 tgattggcga aattggcggc gatgccgaag agcgggctgc cctatttatc aaaaataatg
     1861 tcagcaagcc ggtggtggct ttcatagcag gacaaagcgc acctccgggc aagcggatgg
     1921 gtcatgccgg agccatcatt accagcggaa gcggaagtgc agccgaaaaa attgctgcgt
     1981 ttgagaatgc cggaattccc gtggcacgca aaccgcagga tatccccctg cttgtgaaac
     2041 ggcaactcca atacaacagt taattctgta atacgttgat atatataata ttaacaaaaa
     2101 tccctttgca tcgcaaaagg gatttttgtt gtgccctaat gttcaaacca gccgcaagtt
     2161 tatcctgttg agttttatca acttaaaaaa ataatgtact tttgtcactt ctataataac
     2221 attagaaccg gttatctgaa aaaaaataca tctatggctt taaactatat ctggattgct
     2281 tttattgtca ttgctttttt tgttgcgttg ttgcagatga ttttttttgg caatatttcc
     2341 atttttaacg aggttctgca atctatcttt ggctctgcaa aatccggttt tgaaatatcg
     2401 ctgggactta ccggtattct tactttttgg atgggaatac tcaaagtagc cgaacacgga
     2461 ggagcgctga aagtagtctc gcgcatggtg gccccgtttt tcacccgcat ttatcccgaa
     2521 atacccaaag accatccggc actaagcagt atttttctca atatgtcggc caatatgctg
     2581 gggctcgaca atgcggctac gcccatgggg ctgcaggcta tgaaagaaat gcaggagtta
     2641 aatccgaaaa aagataccgc ttccaatccc atgatcatgt ttctggtgct aaatactgcc
     2701 ggtattgtac tggtgccaat gaccgtgatg atgtatcgta cacagttggg agccgtcaat
     2761 ccggctgatg ttttcctccc gattattgtt gccacacttt gtgcctttat caccggactt
     2821 atcaccgttt ctgttattca gaaaatcaat cttttcaatc gcgtggtaat gggcgtttta
     2881 gccggactgg tgggtttcgt catcgggttg gtatattttt tcagtaccct ctcgcaagaa
     2941 cagattacgg cctattcaag tttctcggct gcctttatta tactttcaat tatcgtcatc
     3001 tttgtgctga tggctgtgaa gaaaaaggta aatgtctata attcctttat cgagggagcc
     3061 aaggaaggtt ttcaggttgc cgtgggcatc attccttacc tggtagcgat gttggtggcc
     3121 ataggcatgt ttcgggcatc gggtacactg gatttgttga ttgacggaat tcgttatctg
     3181 gtagcattaa cgggtgtgaa tactgatttt gtggaagcac tgcccacagc ttttatgaag
     3241 ccattgagcg gaagtggtgc ccggggtatg atgattgaaa caatgacgcg ttatggcgcc
     3301 gattcgtttg tcggacggct ttcgtgcatt attcagggtt ctaccgatac tactttttat
     3361 attctggctg tatattttgg ctctgtaaaa gtaaccaata cccgctatgc ggttatggcc
     3421 ggactttttg ccgattttgt aggaataacg gctgcaattt tgctggggta tttgtttttt
     3481 cattaaaaag aggcccccta aatcccccga agggggactt aaagactttg aaaaagaatt
     3541 atatttcaac taaccgaata aaaatattga aatgttgata tagtaatgta gattttataa
     3601 gtaaaaatgc gaaagttttc attaattaga agatatttgt aggctgttaa acataagtcg
     3661 ttataaaaaa aaggatatta atttaaccat atctacaagt cccccttggg gggatttagg
     3721 gggcttctca tgatccatta cattaccgaa aatatcgaaa tgccggatat taatcaggca
     3781 gttatcaatc gctggataaa ggtgattgcc gctcactggg ggagaaaagt gggaaatatc
     3841 acctacattt tctgctcaga cgcccgcatt ctcgaagtga acaaacagta tcttcagcat
     3901 gattatttca ccgatattat taccttcgat tacagcagcg gaagcgtcat agccggtgat
     3961 atttttatca gtctggatac cgtggccgat aatgcccgtc agcttggagt cctttacaac
     4021 gacgaattaa accgcgttct gatacatgga attttgcatt tgtgcggaca ggacgataaa
     4081 acgcccgaga cacagactga aatgaccgca aaggaaaatg ctgcgcttga aatattgcaa
     4141 aatgagatca actgagagtt tttttcacag aaggtttaag taaccaaaaa gaccttcgca
     4201 tttagcaata aattgttaaa ttaatccgcc taatttctgc ccattacctt atattacaaa
     4261 gattatcact acttttgttt ccaatttcag aaaaaaaaat aaaatttatg gctcaaaaat
     4321 acgttctaca tttattaata actcattatt gttaagaata tgaatgtgtt ttccggaaaa
     4381 accattttat tatgtttttt ctttatttta tttttttcct tcaactcgaa aagtcaggat
     4441 gaatacaggg ctgaaattgg actgcaggga ggtgcaaata tctataccgg tgatgtcaat
     4501 accgtagcaa agccggatta ttttttgcaa aacatgaaga atattcaacc cgatttcggt
     4561 cttttttttc gatatcggtt caatccacgt acggcgttac gcttgggata tgattacagc
     4621 actgtcaatg gatcgtacat atataatgtc aatacctctc aacaattgaa tcagacgctt
     4681 cacattgttg atatgacggg cgaatataat ttttttgatc tcgaaaatga tccgtataag
     4741 cgttatagta agaagtattc gccgtatatt tttgccggac ttggagtaat atacattccg
     4801 gatgctgaaa cattgtttca aaaaaagtat gcaccaaccg ttccatttgg tattggattg
     4861 aaatggaaaa tggctaaaag atggaatttg aatatacaat ggaccaatca tttgctgctt
     4921 agtgataatc tggaaggaaa aatcaaattg gattatggtg aattaataaa agattgggat
     4981 ctgaatggta atcccgtttg gccaaatgat ttatcaccaa tacccacttg gactaagcgc
     5041 atcaatccta tgaataatga cctgctttcc gggttgatga ttggaataag ctttgatttc
     5101 tggaaaaaag gctgcgactg taatcaaagc gataaaatga aaaagaaaac ctctgtattt
     5161 cagcggtaaa aatagtttta tgagtttcaa tgtttagtaa ttttcaattt aattgaacca
     5221 atgtcattca aagagcaatt agacctcaaa agtctgccaa agcatgtggc cattatcatg
     5281 gatggcaacg gacgatgggc taaggaacgg ggatttgacc gaatttacgg acatcagcac
     5341 ggtgtaactt cggtacgtga atgtactgaa gctgctgcag agattgggat caactatctg
     5401 actctgtacg ctttttcgac cgaaaactgg agccgatcgc gcaaggaagt ggatgctttg
     5461 atggaattgt tgattgatac catagaaaaa gaaacaccca cgctgaataa aaacaacgta
     5521 agcctgatgg ccatcggcga tttgtcgcga ttgccacaag gagcatacga aaaacttcag
     5581 cggtgtattc ttcaaactaa aaataatacc ggcctaaggt tggttctggc attgagttac
     5641 tcttcgcgtt gggaaatatc taatgccttc aggctgatat ctcatgaaat ttccgaagga
     5701 aaaattaaga cagacgatat aaccgaagat ttgatttcga actatctgac aacaaaagac
     5761 ataccggatc cggatctgat gatacgtacg agcggagaac taaggatcag taatttttta
     5821 ctctggcagc tggcatacac cgaattgtat ttcaccaata cgcactggcc tgatttcaga
     5881 aaagagaatt tctttgaagc tatcgttgaa tttcaaaaac gtgaacggag attcggtaaa
     5941 taaactgtac aaataagtag acacccaata gatacacatg caatacaaaa aaatacttac
     6001 cttttgttta ctgctctttt tcagtcatct catttcagct cagacagttg ttcccaatga
     6061 ctcagtagtt actccccaga tcgattacaa cgaacccaaa gaatatcaga ttgctgatat
     6121 tcaggtagaa ggtgccgatt cgtacgacga ctttgtcctt atcggatttt ccggcctgca
     6181 agtgggtcaa aaaattgtta tacccggtga tgatattacc agtgccgtga aaaaattctg
     6241 ggaacaatcc atgttttcgg atgtaaaaat tgaactgacg aaagtggaag gcgataaggc
     6301 atggctgact atttacctgc aactccgccc gaaggtgtct gaaattattt acaaaggact
     6361 taaaaaggct gaaatagaag acattgagaa acgcatcggc attctgaagg ataaacaaat
     6421 tacaccgaac atgtcggacc gtgccaccaa atctatcaag aaatacctcg acgaaaaggg
     6481 tttttcaaat gcggatatta aggtttatca gatggatgat cccgccaaac cgggttttgt
     6541 aaaagtggat atagatgtgg ataaaaaact caaaacacgc gttcacaaaa tttacatttc
     6601 gggcaatgac aaactggatg ccctgcagat aaacaaggcg atgaagaaaa ccaatgataa
     6661 caacattatc aatatcttca gaaccaagaa atttgtaaaa gacctctacg aaaaggacaa
     6721 aatagctgcc attgaaaaat acaacgaatt cggttatcgc gatgcctacc tggtgtccga
     6781 cagcgtggtg ccattcgacg acaagtcggt ggatgtttat atgaaattca atgaaggaga
     6841 aaagtattac ttccgcgata tcaagtggat tggcaataca atttatcctt acgaaacctt
     6901 aaatcagttt ctggggataa aaaaagggga cgtttacaat cttcggttgc tcaataaacg
     6961 acttcaggaa gatgaagatg ctgtagcaaa actatattcc aataagggat acttgttttt
     7021 caatattcaa cccattgaag ttgccatcca aaacgactcc atcgatttcg aaattcacat
     7081 ggtggaagga aaaccggcaa ccatcaacga agtaaaaatc accggaaata cacgggtgta
     7141 cgaaaatgtg gttcgtcgtg aacttcgtac tgtgcccgga gcactctaca gtcaggatga
     7201 catcatgcgt acactgcgtg aacttgctca gatgggacat tttgatcctg aaaaattatc
     7261 gaaagatatg atggaaggtg gtctgaaacc taatcctgaa aatggtacgg tcgatttgac
     7321 ctacaaactc gaaacaaaga gcagcgacca ggtggagttt tcggccggat ggggagcagc
     7381 cggtattgtt ggtagtctgg ggcttaaatt cagcaacttt gctattcaga atattttcaa
     7441 accagaaacg tatcgtatcg ttccccaggg cgaaggtcag actttctcca tcaacgcacg
     7501 taccaacgga aattactatt cttcattcag tctttcgttc ctcgaaccct ggttaggcgg
     7561 caaacgacct aattcgctat cagtttcact ttactataca gctacatccg gtatgagcga
     7621 ccgttatctg aatgcttatt ccagtatgtc aagtatgtac ggcggatatg gtggttatgg
     7681 tggatacggt ggttacggtg gttacggttc aggttatgga tatggttcgg gttatggcaa
     7741 tacaagctac gaaaccgaag tggacaaaaa taaatacttc cgcaccctgg gtgcttctat
     7801 cggatatgga aagcgattga gctggcccga tgacttcttt acgttttacg gagaagtagc
     7861 cctgcagcgc tatatgatca acaactggta tcagcaggaa acgaatgtga caaacggtgt
     7921 ttttaataat atcggattaa atctgaccat tgcacgaaat tctatcgaca atccaatttt
     7981 tacacgcaaa ggctctaatt tttcactggg tttacaggta actcctccat actccgcatt
     8041 caacggtaag gattattcca atgtaaatat gaccgatgct gaaagataca agttccttga
     8101 gtatcacaaa tggaaattca gtgccaaaac gttcactcca ttgatggctg tgaataaaac
     8161 tccggtattc atggctcgtg ccgaatttgc ctatgtaggg cattacaata aagatgcacg
     8221 cactccattc ggtacctata tgctcggggg cgacggtatg tcggggtatt cgggttatgc
     8281 caacgaatat gtggctatgc gcggttatga aaccggagct cttactccct acgagtatcg
     8341 cctgaattcg gccggacaaa gtcaggctta cgctcaacgg gcttatctgt acaacaaatt
     8401 caccatggag cttcgcgtac ctatttcact ggaacaatcg gccaatatct gggcacttgg
     8461 atttttagag gcgggtaaca gcttcaataa aatatctgat tacaatccct tcaatctgaa
     8521 gcgttcggcc ggtgtgggcg tgcgtattta cctgcctatg tttggtataa tgggcatcga
     8581 ctggggttac ggatttgatc cgcaggtgaa tgagcaaaaa gcccatggaa gccagttcca
     8641 ctttgtaata ggtcaggaat tataaaaaga gagatattat cctgattgta attatctgaa
     8701 tatcaactca aaaaagtttg gcaaagattt tgatatgtat attacacgta tttgtaatat
     8761 caaactgaaa aaacttactc aacgatgaaa aaattacttt taaccctctg tctgttggca
     8821 ggaataggta ttggcacaca ggcacaaaag tttgcctcgg tggatatgtc ctatattatg
     8881 aaaaacatcc ccgcgtatga aagcgcgaat gatcaactga atcaggtttc gaaaaaatgg
     8941 cagacagaag tagaaaccag catgaaagat gttcagcaaa tgtacaagaa ctatcagaca
     9001 gaacttgtat ttatgtcgga cgatatgaaa gtgaaacgcg aagaagaaat tgtagccaaa
     9061 gaaaaagagg ctcaggaact aaaacgcaag tacttcggac ccgaaggcga attgttcaaa
     9121 aaacgggaaa gtctgatgaa accgattcag gatgaagtgt ataacgccat tcaggagata
     9181 agcaaagaca aggattttca actggtattt gacaaaagtt cagccatgag tgccattttt
     9241 atctcaccaa aactggatat cagcgacgat gtcttgaaaa aattgggtta ttcaaaataa
     9301 ttatatactt ttgtaagcca aaaaaataaa tataaatcac cagaaattaa tttaataaaa
     9361 agaaaatgat taaaaaagtt gctttgttct tgattgcctt gttaccgctg ggagcaatgg
     9421 cgcaaaacgt aaaattaggt cacgttaatt ctcaggaaat aatctcagta atgcctgaac
     9481 tcgaaacaat tgaaaaaacg ctgaaagaac ttcaggatca atgggaaaaa gaattactga
     9541 aaatgcgtga agaatataac acgaaagtaa aagaattcgt tgataaacag gctacgatgc
     9601 ccgaaaccat taaaacggcc cgtcagtcag aaattgccga aatcgaacag cgtatcacca
     9661 ctttcaatca gacagccaat gctgatgttg tgaaaaaaca acaggaactg gttaaccccg
     9721 tgcttgataa agtgaaaaaa gccatcagcg atgtagctac cgaaggcgga tatacctata
     9781 tttttgatat ttcaacacag ggagttgttt tccaggccaa aacagccaat gacattactc
     9841 cgctggtaaa gaaaaaatta ggtgtga
//
LOCUS       DAOPZK010000073         9829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_8097_length_9829_cov_27.139656, whole genome shotgun sequence.
ACCESSION   DAOPZK010000073 DAOPZK010000000
VERSION     DAOPZK010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_9829_cov_27.139656"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1513
                     /locus_tag="PKH68_05795"
     CDS             23..1513
                     /locus_tag="PKH68_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNX12485.1"
                     /translation="MAKEMKSLAKDTVIYGGTNIAAKFVSWMLVVLYTYTLPNPAEYG
                     VVTNLYAWTSILIVALTYGMETGFFRFANKDTANAKKVYGNTLISIGFTSLLFAIIIL
                     VFQQPIANTLGYRNHPEYIWMLGLSVAMDAFSCIPFVYLRFIKRPYVFAGMKLLYVGL
                     NVVLNIFFLVLCPFLMKVAPSTISWFYRPDYGVGYIFISNIISTTIQTLILSKYIFQA
                     EFKFDSQLLKQMLKYSYPLLFLGIAGIANQNLDKMIFPFLQVGTKGGNDLGIYGAVSK
                     LALVIMMFTQAFRFAYEPFVFAKHKDEDSSQTYSEAMKFFVILSFLIFLGMILYMDII
                     KYLVPSEYWVGLKVVPIILFSFIFQGIYFNLSIWYKLKDKNHYGAWFAAIGTLIVFVG
                     NIIFVPRYSYWGSAWSAFAGYFIVMLLSYFFGQKFMPIDYHLKKLGIYLALTLVLFGI
                     SSFIETPYLIVNLALKTVLMAVFIVFFIKKDMPLKEIPYLNRFLKK"
     gene            1516..2229
                     /locus_tag="PKH68_05800"
     CDS             1516..2229
                     /locus_tag="PKH68_05800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX12486.1"
                     /translation="MKLHIITPVKNSFGTTIRTIESIVNSENNADLHYTIYNDFSDDE
                     TTFLLQEAAGKYGIELVHLKSLTTHPSPNYRLVLQLAQKKAIEDNAHLVIVESDVVIQ
                     PATIQKLMLQAAKVKNPGLIAAVTTDETGEINFPYLFARSYRKGTVETGKRLSFCCTL
                     LTNRFLNAYNFENLDSEKTWYDIYISHKSTKLGFKNYLLTNVPVLHLPHSSRPWKMLK
                     YTNPLKYYWKKYTKGLDKI"
     gene            2230..3342
                     /locus_tag="PKH68_05805"
     CDS             2230..3342
                     /locus_tag="PKH68_05805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNX12487.1"
                     /translation="MTGTDNSIQHNKKEENSAVFPLVSVIIPVYNLELYLAETLDSVL
                     SSTYPNVEVIVMDDGSRDKSAEIATNYALKDKRIQVYTQPNGGASSARNNAIRLAHGK
                     YILPVDGDDTISANYIEEAVNVLENNEEVKVVSREATYFGEKTGRWKFPPFSYNLLAR
                     RNIIDVCSMYRKSDWEKAGGYCEEIPGREDWDFWLSLFKSGGKFVRLPLVGLHYRIHS
                     GSKRVRTRGLKKELIDTLNLRHKAFFYAQLNGKLHYQRTHSRKMNNFRHFFLPENIFV
                     NPAFDEYEDAVYLANEKPAVRSEMLRKMRIPAANSIRFTGFIEKKYLLPGRKIATSQA
                     RKLYEQHSAEKNSRYLGYYEQQTSPCTLKSYLITLD"
     gene            3349..4161
                     /locus_tag="PKH68_05810"
     CDS             3349..4161
                     /locus_tag="PKH68_05810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX12488.1"
                     /translation="MKASLIIAVYKNAVYLKAVLDSLQYQTEKDFEIIIAEDGESADM
                     KAFIEQYPFEQPYKHITQKDEGWRKNQCLNKAIGVSEGNWLIFIDGDCVLHHRFIEMH
                     LRAAEEGKILAGKRVKLDQELTNFILGDSKNIRKIEKTLYGKLIAGKGKIKFPEEGIF
                     ISPDGMLGFIPRMRKNDKLLGCNMSFSRKAAYAINGFDEDYQLPAVGEDYDLAWRFRA
                     AGYQLVSVRNLAVQYHLNHPEGYNQDFNLDLCRQKQAKNEFVCKNGLIKHTV"
     gene            4166..5059
                     /locus_tag="PKH68_05815"
     CDS             4166..5059
                     /locus_tag="PKH68_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX12489.1"
                     /translation="MVSVIICSKHEKLSESVRQNIDLTIGVPYEIVCINNSRREYSIF
                     SAYNKGIAQSKYPFLCFVHEDVIFQTENWGKRIVSQLDNSTTGLIGVAGSNTVFNVPS
                     SWSSSPEKYINIIQSGKHSAHVRFPIAHNNDKQEVILLDGVFLCANRNLFSKIAFDEN
                     LKGFHGYDLDICLQSASAGFQNYVVFDVLLEHYSKGFRNKSFFENLIKIYRKWDKYLP
                     VYDKSRYATVDILNSEEKAIDYLFIKMIKRGFVAAEIKSAITFYIEKIKSEKLQQKYR
                     HISRKIFFLQLVYKPFTRLYR"
     gene            5102..5869
                     /locus_tag="PKH68_05820"
     CDS             5102..5869
                     /locus_tag="PKH68_05820"
                     /inference="COORDINATES: protein motif:HMM:NF017515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HNX12490.1"
                     /translation="MECINNPSELTYQHQVPFVVWCYWEGAPMNANRLLSFEYLQKNI
                     HVPVCLITKDNLSAFIKPEFPLHEAFPYLSAVHRSDYIRAYLMHFYGGAWHDIKATEV
                     SFRDCWNEFENPDIYLIGRPENQKGAARVYDEHNRYMPDYWKDLVAVPAWIARGNTAM
                     TSEIYAGFLKLLDNNLEALKKHPAKHPREKFIQPKNFFHALIIRLKHLYSGRNPNYPL
                     PWTVFGNIFHPVVYEYREHVSYNLPVNKIKNAGIYHR"
     gene            5976..6764
                     /locus_tag="PKH68_05825"
     CDS             5976..6764
                     /locus_tag="PKH68_05825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX12491.1"
                     /translation="MRSEPKVSVITPIYNYEKYIGDTLRCLQKSSYQNWECIVMDSDS
                     TDNSAAVVQEMAREDDRIKYFHHTKLPIPQAKNNAVAHSSGKYLLPLDSDDLIAPEYM
                     SEAVEILENNAAVKLVYCAGVYFGDKKGKWKLAEYSFHQLLLKNCIHNSALFRRTDFD
                     AVRGYNPDMITSEDWDLWISLLENGGEVAYIDKEYFYYRKHVHSFTDNNQDKIDQTLK
                     QIYQNHKEQYEKMLENPIRLLREHEKYKKGYNQLRRLTFRKQIP"
     gene            complement(6729..8174)
                     /locus_tag="PKH68_05830"
     CDS             complement(6729..8174)
                     /locus_tag="PKH68_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNX12492.1"
                     /translation="MKLLLKKSYFSLLILFFTPLAIGFIYRLYSAQIFVNEPHTEMFG
                     NILLTGFAVLSLIIFLTEIITHRKILQPLFSVDTFVFISLTLLLVLSVFKKEVGIYAT
                     GFFVLFALMSLFVHEKFYTLNNVYFFIFLYAVCLMLGTIYTPRGFYFPEMTYTFYVLP
                     LAFSLFRLKKQSLLLILKIVFRIMAVYMAVSIVYWWFNLLVLNMSALEWITTKFPPAY
                     FYVGKWGHYSHPSYISLVLFSTLISGFYLNFKKDKDVTLSKFELITYFGLCLGFVLIM
                     ESRIGLVELIIIIVISLLYYIRLKTNYLKHALVLAVLAGMIILMAGENKFSRLIHDGV
                     RKADFTLAVNYIKEHPVAGAGYHQQRAALEYQDKKMTDVTRPADAPPITYTHNQFLGD
                     MVQFGIPGLLPLLILLAGLVWYAFKSRSYLLQLYMLIYLLFMLIEEPLYAQEGITRFM
                     AFLCLFIHISECDKPVKVHRLRNLFSKGEPA"
     gene            complement(8193..9020)
                     /locus_tag="PKH68_05835"
     CDS             complement(8193..9020)
                     /locus_tag="PKH68_05835"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HNX12493.1"
                     /translation="MIKNITIYGFITFFCLFSCLQAGCTDELSVNPKLTVIIKADDLI
                     DYSPNWQRFVSILQKNDISAAVGVTSDLVASKQATIVKIRELSSLTRKDKKPLIEFWN
                     HGYDHSREKGTRTEFNNTDFNLQYQHIQLAQQFFADSLRLTAKTFSAPFNNSTSTTLK
                     AVTSFPEINVIMRSEKHEKYSLTRPWADPNKKRIKSDNKICLNIRFLKLYDVPLTDIE
                     NYFKVEINDSYLVIQIHTNPWEDDDFADFQRMIDFLKDKKVTFMTPDEYYQFLKSTK"
     gene            complement(9030..9674)
                     /locus_tag="PKH68_05840"
     CDS             complement(9030..9674)
                     /locus_tag="PKH68_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNX12494.1"
                     /translation="MSKKKQKYYTVWEGENPGVYTSWSECEKQVKGFDEAKFKSFESL
                     KEAQDAFLSSWWLYIGKNAPNQKPTGNYKLLPPAEQPNLNSIAVDAACAGNPGKMEYR
                     GVDVQTGEEIFRQGPFENGTNNIGEFLAIVHALALFNKTNPEIPVYTDSMTAMKWIKN
                     KKCRTRHLEINNNTNLFELIRRAEKWLQEHTYSNPLLKWNTEIWGEIPADFGRK"
     gene            9756..9827
                     /locus_tag="PKH68_05845"
     tRNA            9756..9827
                     /locus_tag="PKH68_05845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9789..9791,aa:Arg,seq:cct)
BASE COUNT         3071 a         1879 c         2023 g         2856 t
ORIGIN      
        1 atacaaaaaa gccaattaat ttatggcaaa agaaatgaaa tcgctggcga aggacaccgt
       61 aatttatggc ggtaccaata ttgctgcaaa atttgtaagt tggatgcttg tggtacttta
      121 tacctacacg cttccaaatc ctgctgaata tggtgtagtt accaatttat atgcctggac
      181 ttctatatta attgttgctt taacttatgg aatggaaacc ggttttttcc gttttgcaaa
      241 taaagacacg gcaaatgcta aaaaagttta tggtaacaca ctgatttcca taggttttac
      301 aagtttactt tttgcaatta tcattcttgt atttcaacag cctatcgcaa atacattggg
      361 ttacagaaat catcctgaat atatctggat gcttggatta agcgtggcga tggatgcttt
      421 ttcgtgtatt ccattcgttt atttgcgttt tattaaacgt ccgtatgtgt ttgcaggaat
      481 gaagttgtta tatgttggtc tgaatgtagt attgaatata ttctttttgg tgctttgtcc
      541 ttttttgatg aaagtagctc cttctacaat ttcctggttc tatcgcccgg attatggagt
      601 aggttatatt tttatttcaa atattatttc cacaaccatt caaacgctga tattaagcaa
      661 atatattttt caagctgaat ttaagtttga cagtcaatta ttgaagcaga tgcttaaata
      721 ttcctatccc ttattgtttt tgggaatagc tggaattgcc aatcagaatt tagacaaaat
      781 gatatttcct tttctgcagg taggaacaaa aggaggaaac gatttaggta tttacggagc
      841 agtcagtaag ttggcattgg tgattatgat gtttacgcaa gcgtttcggt ttgcttatga
      901 accctttgta tttgccaaac acaaagacga ggacagttca caaacgtaca gtgaagcaat
      961 gaaatttttt gttatacttt cattcttaat ttttttagga atgattttat atatggacat
     1021 cataaaatat cttgtaccaa gtgaatactg ggtgggatta aaagttgtac caataattct
     1081 gttttcgttt atttttcagg gtatttattt taatctttct atctggtata aactgaaaga
     1141 taaaaatcat tatggagcgt ggtttgccgc tatcggaacg ttgattgtat ttgttgggaa
     1201 tattattttt gtgcccagat atagttattg gggctcggca tggagtgctt ttgcaggata
     1261 ttttatagtg atgctcttgt catatttctt tggacagaaa tttatgccga ttgattatca
     1321 cttaaagaaa cttggaattt atttggctct aacgcttgtg ttgtttggca taagctcatt
     1381 tattgaaaca ccttatttaa ttgtaaattt agctctgaaa actgtgttaa tggccgtatt
     1441 tattgttttc tttatcaaaa aagacatgcc ccttaaagaa ataccttatc tgaaccgatt
     1501 tttgaagaaa taaaaatgaa actgcatatc ataacgccgg taaaaaactc tttcggcacc
     1561 accatccgga cgattgaaag tattgtaaac tcggaaaaca atgcggattt acactacacg
     1621 atttacaatg atttcagcga cgatgaaacg acttttctgt tgcaggaagc ggccgggaaa
     1681 tacggcatcg aactggtgca tctcaaatcg ttgaccaccc atccatcgcc caattatcgt
     1741 ctcgtattgc aactggctca gaaaaaagcc atagaggata atgcccatct ggtcattgta
     1801 gaatcggatg tggtgataca gcccgcaaca attcaaaaat tgatgctcca ggctgccaaa
     1861 gtgaaaaatc cggggctgat agcagccgtt acaaccgatg aaaccggtga aataaatttt
     1921 ccgtacctgt ttgcccgcag ctaccggaaa gggactgtgg aaaccggcaa acggctgagt
     1981 ttttgctgta ccctcctcac caaccgattt ctgaatgcct ataattttga aaatctggat
     2041 tccgaaaaaa cgtggtacga catctacatt tcgcacaaat caacaaaact gggcttcaaa
     2101 aactacttgc tgaccaatgt acccgtactg catcttccac acagcagccg cccgtggaaa
     2161 atgctgaaat ataccaatcc gctaaagtac tactggaaaa aatataccaa aggattagac
     2221 aaaatttaga tgacaggcac cgataattcc atacaacaca ataaaaaaga agaaaattca
     2281 gccgtttttc ctctggtttc cgttattatt cctgtttaca atttggaatt atacctggcc
     2341 gaaacactcg actccgtgct gagttccact tatccgaatg ttgaggtaat tgttatggat
     2401 gacggttccc gtgacaaatc agccgaaatt gccacaaatt acgcattaaa agataaaaga
     2461 atacaagttt atacccagcc gaacggtgga gcttcatcag ccagaaataa cgcaatccgt
     2521 ttagcacatg gaaaatacat tttgcccgtt gatggagatg atacaatctc ggcaaattat
     2581 atagaagaag ctgtcaacgt attggaaaac aatgaggagg taaaagtggt aagccgggaa
     2641 gccacttact tcggtgaaaa aacagggaga tggaaatttc caccattcag ctataacctc
     2701 ctggcacgca gaaatataat agacgtatgc tccatgtacc gaaaatcaga ctgggagaaa
     2761 gcgggaggct actgcgaaga aattcccggg cgtgaggact gggatttctg gctttcgctt
     2821 ttcaaatcgg gtggaaaatt tgtacggtta ccgcttgtcg ggcttcatta cagaattcat
     2881 tccgggtcaa agcgggtgag aacccggggc cttaaaaaag agcttattga tacgctaaac
     2941 ctccgccaca aggctttttt ttatgcccaa ctgaacggaa aactgcatta ccagcgtact
     3001 cattcccgaa agatgaacaa tttccggcat ttttttctgc cggaaaatat ctttgttaat
     3061 ccggcatttg acgaatatga agatgcggtg tatctggcca acgaaaaacc cgcagttaga
     3121 agtgaaatgc tcaggaaaat gagaattcca gctgcgaatt ctatccggtt taccggtttt
     3181 attgaaaaaa aatatttgtt gcccgggaga aagattgcca catcgcaagc ccggaaactt
     3241 tatgagcaac attcagctga aaaaaattcg aggtatttgg gttattatga gcagcaaacg
     3301 tcaccctgca cactgaaaag ttacctcatt acgttagatt aaaaatcgat gaaagcctca
     3361 ctaatcatag ctgtttataa aaatgcagtg tatctgaagg cagtgctcga ttcgctgcaa
     3421 tatcagacgg aaaaagactt tgaaatcatc attgccgaag atggtgaaag tgccgatatg
     3481 aaagcgttta ttgagcaata cccgttcgaa cagccctata aacacatcac acaaaaagat
     3541 gaaggctgga gaaaaaatca atgcctgaat aaagccatcg gggtatcgga gggcaactgg
     3601 ctgatattta tcgatggcga ctgcgtgctg catcaccggt ttatagaaat gcacctcaga
     3661 gcagctgaag aaggtaaaat tttggccgga aaacgggtta agctggacca ggaactcacc
     3721 aacttcatcc tcggcgacag taaaaatatt agaaaaattg aaaaaacact ttacgggaaa
     3781 ctgatagccg ggaaagggaa aataaaattt ccggaggaag gaatatttat ttcgcccgac
     3841 ggaatgcttg gttttattcc ccgtatgaga aaaaacgaca agctcctggg ttgtaatatg
     3901 tcattcagcc gaaaagcggc ttatgccatc aacggatttg acgaagatta tcagcttccg
     3961 gctgtgggcg aggattacga cctggcgtgg cgcttcagag ctgccggata ccaactcgta
     4021 tcggtgcgta atctggcggt gcagtatcac ctgaatcacc cggaaggata caatcaggac
     4081 tttaatttag atttgtgcag gcaaaaacaa gcgaagaatg aatttgtgtg taaaaacggc
     4141 ttaatcaaac atacggtcta acaacatggt atcagttatt atctgctcga aacatgaaaa
     4201 attgtcggaa tctgtaagac aaaatataga tttgaccatt ggagtaccgt atgaaattgt
     4261 ttgtatcaat aattcgcgca gggaatattc cattttttcg gcttacaaca aaggcattgc
     4321 ccaaagcaaa tatccctttt tgtgttttgt acatgaggat gtgatttttc aaacagaaaa
     4381 ctggggaaaa agaatagttt cacagttgga taattcaact acagggctga ttggggtggc
     4441 aggaagcaac acggtattca atgttccatc atcttggtcg tcatctcccg aaaaatatat
     4501 aaatatcatt cagtccggaa aacactcagc acatgttcgg tttccgatag cgcataataa
     4561 tgacaaacag gaagtcatat tgctggatgg tgttttttta tgtgctaatc gcaatttatt
     4621 ttcaaaaata gcctttgatg aaaatttgaa aggatttcac ggttacgatt tagatatttg
     4681 ccttcaatct gcttctgcag gatttcagaa ttatgtggtt tttgatgttt tattagagca
     4741 ttattccaaa ggatttagga ataaaagttt ttttgaaaac ttaataaaaa tctaccggaa
     4801 atgggataag tatctgccgg tttatgataa aagccgatat gccacagtgg atattttgaa
     4861 cagtgaagag aaagcgatcg attatttatt cattaaaatg ataaaaagag ggtttgtagc
     4921 tgcagaaata aaatcagcaa ttacgtttta cattgaaaaa ataaaatccg aaaaattaca
     4981 acaaaaatat cgacacatca gccggaaaat tttctttctt cagttggtct ataaaccatt
     5041 cacaagatta taccgctgat cattattcta ttataaatct gacagtctgc tatttacaac
     5101 tatggaatgt atcaataatc cttcggaact cacctatcaa catcaggttc catttgtggt
     5161 atggtgctac tgggaaggtg ctccaatgaa tgcaaacagg ctgctgagtt ttgagtattt
     5221 acaaaaaaat atacatgtgc ctgtttgctt gataaccaaa gataatctgt cagcatttat
     5281 taaacctgaa tttccacttc acgaggcttt tccatatctg agtgcagtac atcgttcgga
     5341 ttacatcaga gcttatttaa tgcactttta cggaggtgcc tggcacgata ttaaagctac
     5401 tgaagtttct ttccgggatt gctggaacga gttcgaaaac ccggatattt atttaatcgg
     5461 tcggcccgaa aatcagaaag gagctgccag ggtgtacgat gagcacaaca gatacatgcc
     5521 cgattactgg aaggacttgg ttgccgtacc ggcctggatt gcacggggaa atacggcgat
     5581 gacatcagaa atctatgctg ggtttttaaa actgttggat aacaatctgg aagccttaaa
     5641 aaaacatccg gcaaagcatc cgcgtgaaaa atttattcag ccaaaaaact tctttcacgc
     5701 actgattatt cgtctaaagc acttatattc tgggagaaat cccaattacc cgcttccgtg
     5761 gactgtattc gggaatatat ttcatccggt ggtttatgaa taccgggaac acgtttccta
     5821 taatttgcct gtaaataaaa taaaaaatgc aggtatctat catcgctaaa tgagtatctt
     5881 tgacttacga actttagaat taatatattc tgcaatatat atcccgctta aaaaataatc
     5941 gttcaggatt gattaaaaat taataaatac cagctatgcg ctcagaaccc aaagtgtctg
     6001 tcatcactcc catctataac tacgaaaaat atatcgggga tactctccgg tgccttcaga
     6061 aatccagtta tcaaaattgg gaatgtatag tgatggatag cgattcgacc gataactcgg
     6121 ccgctgtggt acaggaaatg gcccgggagg atgatagaat aaagtatttt catcatacta
     6181 aattgcctat tccgcaagct aaaaacaatg ccgtggctca ttcctcgggc aaatacctgc
     6241 tgccgttgga ttccgacgat ctgatagcac ctgaatatat gtctgaagca gtagaaatac
     6301 ttgaaaataa tgctgcggta aaactggttt actgtgcggg tgtttatttc ggagataaaa
     6361 aaggaaaatg gaaacttgcg gaatatagtt tccatcagtt gttgctgaag aattgtattc
     6421 ataattcagc tcttttccgg aggacagatt tcgatgctgt acggggttac aatcccgata
     6481 tgatcacgag tgaagactgg gacttgtgga ttagtctgct cgaaaacgga ggcgaagtgg
     6541 cttatattga taaggaatat ttttattatc gtaaacacgt ccattcgttt accgacaata
     6601 atcaggataa aatagaccaa acgctgaaac aaatctatca aaatcacaaa gagcagtatg
     6661 aaaaaatgct cgaaaatccc attcggttat tgcgtgaaca tgaaaagtac aaaaaaggct
     6721 acaatcagct acgccggctc acctttcgaa aacaaattcc ttaagcggtg aactttcacc
     6781 ggcttgtcgc actcactgat atgaataaac agacacagaa aagccataaa tcgggtgatg
     6841 ccttcctgcg catacaacgg ctcctctatg agcataaaaa gcagatagat aagcatataa
     6901 agctgtagca ggtaacttcg ggacttaaaa gcataccaca ccaacccggc aagcaggatc
     6961 aagagcggaa gcagaccggg aattccaaac tgcaccatat cgcccaaaaa ctgattgtga
     7021 gtataggtaa tgggcggtgc atcggccggc ctggttacgt cggtcatctt tttatcctga
     7081 tattccaacg ctgcccgctg ctgatggtaa cccgcgcctg ctacgggatg ttccttaata
     7141 taattgacag ccagcgtgaa atcggccttt cgtactccat cgtgaattaa ccggctgaat
     7201 ttgttttcgc ccgccatcag tataatcata cccgccaaca ccgccaacac caaagcatgc
     7261 ttaagatagt tggttttcag tctgatgtaa tacaacagac tgattacaat gataataatt
     7321 aactccacca acccgatgcg gctttccata atcaacacaa aaccaagaca taagccaaag
     7381 taggttatca gttcgaattt ggacagagtg acatccttat cttttttgaa attgaggtaa
     7441 aaacccgaaa ttaacgtcga aaataatacg agtgatatgt aactcggatg agaataatga
     7501 ccccacttgc ctacgtagaa ataagccgga ggaaatttgg tagtaatcca ttcgagggca
     7561 gacatattca aaaccagcaa attgaaccac cagtaaacga tggaaaccgc catataaacg
     7621 gccatgatgc gaaatactat ttttaaaatc agcagcaggc tttgcttttt gaggcggaaa
     7681 aggctgaaag ccagcggcaa aacataaaaa gtataagtca tttcgggaaa ataaaaaccc
     7741 cgtggtgtgt atatagtgcc cagcataagg catacggcat agagaaatat gaaaaaatac
     7801 acgttgttga gggtgtaaaa tttttcatgt acgaataagg acatcagggc aaacaacaca
     7861 aaaaaaccgg tggcatagat tccgacttct tttttgaata cagataaaac cagtaagagg
     7921 gtaagcgaga tgaagacaaa cgtatcgact gaaaacagag gttgaaggat tttacggtgg
     7981 gttatgattt ctgttaaaaa aattatcagt gacaaaacgg caaaaccggt caacaaaata
     8041 ttgccaaaca tttcggtatg aggctcattt acaaatattt gtgcggaata aagccggtaa
     8101 ataaacccta tagccaatgg agtaaaaaag agtatcaaca gtgagaaata cgattttttg
     8161 agcaataatt tcatgcggac ggttcggttt tgttattttg tacttttcaa aaactgataa
     8221 tactcatcag gtgtcataaa tgtaactttt ttatctttca gaaaatcgat cattcgctga
     8281 aaatcggcaa aatcatcgtc ttcccatgga tttgtatgaa tctgaatcac gaggtacgaa
     8341 tcattgattt ccaccttgaa atagttctct atatcggtta atggcacatc ataaagtttt
     8401 aggaagcgaa tattcaggca aattttattg tcggatttta ttcgtttctt attcgggtct
     8461 gcccagggac gggttaatga atatttctcg tgcttttcgc tgcgcatgat tacattgatt
     8521 tcgggaaaag aagtaacggc tttcagcgta gtggaggttg agttattgaa gggtgcgctg
     8581 aatgttttgg cagtgagacg cagcgaatcg gcaaaaaact gctgagccag ctggatgtgt
     8641 tggtactgca gattaaaatc cgtattatta aattcagttc gcgttccttt ttctcttgaa
     8701 tgatcataac cgtggttcca gaattcgatg agcggctttt tgtctttgcg cgttaggctt
     8761 gaaagttccc tgattttgac aattgtagcc tgttttgatg ccaccagatc agatgttaca
     8821 cccactgcgg ctgatatatc gtttttctga agaatggaaa caaatcgctg ccagttgggc
     8881 gagtagtcaa tcaaatcatc cgctttgatg atgactgtca acttcgggtt cacagacaat
     8941 tcatccgtgc atcctgcctg caggcaggag aacaggcaga aaaaagtgat aaaaccgtat
     9001 attgttatat ttttaatcat tttccagcat cattttcgac caaaatcggc gggtatttct
     9061 ccccagattt cagtattcca tttcaataaa ggattagaat acgtatgttc ctgaagccat
     9121 ttttcagctc tccgtatcag ttcaaacaga tttgtattgt tgttaatttc aaggtgtctg
     9181 gtgcggcatt ttttgttctt tatccatttc atggccgtca tactgtcggt ataaacgggt
     9241 atttcgggat ttgttttatt aaaaagagcc agggcatgaa caatagccag aaattcgccg
     9301 atattattgg ttccgttttc gaaaggtccc tgccggaata tctcctcccc ggtttgcaca
     9361 tccacgccac ggtactccat ttttccggga ttacccgcgc aggcagcatc caccgctata
     9421 ctgttgaggt ttggctgctc tgccggcgga agtaatttgt aatttcctgt gggtttttga
     9481 ttgggtgcat ttttgccaat gtagagccac cacgacgaca gaaatgcatc ctgagcttcc
     9541 ttcaagcttt cgaatgactt gaattttgct tcatcaaatc ccttcacctg cttttcgcac
     9601 tcactccaac tcgtgtaaac tccgggattt tcaccttccc aaacggtata atatttttgc
     9661 ttttttttac tcatctcaat gtattaacta tttcgaaacg caaaaaatga agtttttatt
     9721 attcaacaaa aagatgtaat tttgcactga taaaaggtct cgtagttcaa tggatagaac
     9781 ggaagtttcc taaactttaa attcgggttc gattcccggc gggactact
//
LOCUS       DAOPZK010000074         9640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_8313_length_9640_cov_24.653104, whole genome shotgun sequence.
ACCESSION   DAOPZK010000074 DAOPZK010000000
VERSION     DAOPZK010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8313_length_9640_cov_24.653104"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..1471)
                     /locus_tag="PKH68_05850"
     CDS             complement(320..1471)
                     /locus_tag="PKH68_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNX12495.1"
                     /translation="MAKILVIDDERSIRNTLKDILEFEKHKVTLAENGKQGLETALNE
                     FFDIIFSDIKMPEMDGIELLEQLREKEVEAPVIMISGHGNIETAVDCIKKGAFDFIEK
                     PIDLSRLLVTVRNALEKNNLVTETKTLKKKVAKKLQMIGNSPSIEKVREMIRKVAPTD
                     ARVLITGANGTGKEVVARLLYEYSNRVQGPYVEVNCAAIPSELIESELFGHEKGSFTS
                     AVKQRIGKFEQADNGTIFLDEIGDMSLSAQTKVLRALQESEISRVGSDKNIKVNVRVI
                     AATNKNLKKEIELGNFREDLFHRLNVIPIHVPTLDERKDDIPLLITHFSELICAEQGI
                     QLKHFDAKAVKALQERSWTGNIRELRNVVERLIILGGNKITKEDVEMFS"
     gene            complement(1641..2909)
                     /gene="purD"
                     /locus_tag="PKH68_05855"
     CDS             complement(1641..2909)
                     /gene="purD"
                     /locus_tag="PKH68_05855"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNX12496.1"
                     /translation="MNILLLGSGGREHAIAWKIAQSPKLTKLFIAPGNAGTNSTGENI
                     AVNISDFQAVKRVVLENNIGMVVVGPEEPLVNGIVDFFETDDELKSIPIVGPNRLAAQ
                     LEGSKDFSKQFMIRHNIPTAGYLSVTSENLNAGIDFLKKLKAPYVLKADGLSAGKGVL
                     IISDLDEAIEELNNMLGGKFGKASQTVIIEEFLSGIECSVFVITDGKNYKILPEAKDY
                     KRIGEGDTGLNTGGMGAVSPVSFADKAFMQKVDERIIKPTVDGLRSEEITYKGFIFFG
                     LIKVNNEPYVIEYNARMGDPETEVVMLRLKSDLVDLLEGVATGNLNKKTVEFDERSAV
                     TVMLVSGGYPEAYEKGKVITGLEDVKESIAFHAGTTEKDGNILTNGGRVIAVSSYGKN
                     KDEALELSYKNAKLIDFEGKYFRRDIGFDL"
     gene            complement(2942..4147)
                     /locus_tag="PKH68_05860"
     CDS             complement(2942..4147)
                     /locus_tag="PKH68_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PNGase F N-terminal domain-containing protein"
                     /protein_id="HNX12497.1"
                     /translation="MLLIGIQAVNAKTAPARGNFQARVFSDLNIVFNPSKFPDGLNNS
                     DSIICLANGRIAIKKIKIPDYKRNVRVTAKLKLTSNGDPWDKTGSCFIIPKESMINMM
                     TIAKGEHQYPQIDSTKLEKLVGVAAGKDYLPTVELMRFMTPFGVGYFSADKDSTAYKR
                     RPVYIDGWAQESVWQQDITDLYPLLQNEAYIGIYIDTWTDKGYKVNLEINFIESTIKQ
                     DVMPVRKVLPLINTVYYIGQTYPDIFSRRDVTVPFTIPVKAKNIRLKYIVTGHGGHSG
                     GDEFVQRENILSVDGAVVHRFTPWRNDCASFRRFNPTSGVWLKKRRIAYISEEGRAEK
                     EIEEPIASSDLSRSNWCPGSDVPPAIIPLDDIKAGEHTLTISIPQAQAIDNNKLNHWL
                     VSSYLVWEE"
     gene            complement(4168..4971)
                     /gene="cdaA"
                     /locus_tag="PKH68_05865"
     CDS             complement(4168..4971)
                     /gene="cdaA"
                     /locus_tag="PKH68_05865"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNX12498.1"
                     /translation="MLFNIGVKDIIDIILTAFLLYGAFKLLKRSGALNVFLGIMTFII
                     IWFVVSKVLRMELLGVILDGLVNVGAFALIVLFQEEIRRFFSRLGSRKNWGILNYFKK
                     FLKGSENEQIRTDYDLIQIVIACRNLSKTATGGLIIVTRTNDLEFYTQSAEIIDARIS
                     SRLIENIFFKNSPLHDGALIISNRRLRAAACILPVSRNQSIPKRLGLRHRSALGISEI
                     SDAIAIVISEETGHISWAINGKLTINGKPEELEHVLTEEITEYNKAKNV"
     gene            complement(5056..5907)
                     /gene="folP"
                     /locus_tag="PKH68_05870"
     CDS             complement(5056..5907)
                     /gene="folP"
                     /locus_tag="PKH68_05870"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNX12499.1"
                     /translation="MNKIPFQLNLNGKLFDLSKPAVMAIANITPDSFFSGSRLFSDKD
                     VLNRVEKALAQGASIIDVGAYSTRPSAPHVTEEEELERLRSPLKAIRKHFPDAVISLD
                     TFRSGVARWGVEVCGVDIINDISGGTLDNLMFETVASLNTSYILMHTSGTPQTMQQLT
                     HYDNMMSEILHFFEKRVAQLVHLGVKDIIIDPGFGFAKTLDQNYELLAKMSYFKELNL
                     PILYGISRKSMIYKLLDTTPEEALNGTTALNMLGLLNGADILRVHDVKEAVETIKIFE
                     KYKTACE"
     gene            6179..7735
                     /locus_tag="PKH68_05875"
     CDS             6179..7735
                     /locus_tag="PKH68_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX12500.1"
                     /translation="MKLNNLATFLTLAFAAVVVASCGSKSGASSGFGSGKKSAISNTT
                     GWRYNDEKATGFGVKKNYKPQVPTGMIAIEGGTFTIGEKGEVVTGERNNLRRRITVMS
                     FYMDQYEISNVNWREYTHWMKTVFQQTAPRLVERTQPNAEVWREPLAYNEPLLEHYFT
                     HPAFDEYPIVGVTWEQAMDYCQWRTDRVNELALINAGIIDAPDFSSLKKITNADSIAN
                     NFVFNTQKYIYHRNYNPKAGKKSMKDIYGQTRKADMSDGIMTSNYRLPTEAEWEFAAY
                     AIRSDKKDGLVKEGRVYPWNGSQLRNSKKGKYEGEMQANFVRGRGDMMGTSGSLNDKA
                     AITAPVTAYFPNEFGLYNMAGNVNEWVLDVYRSTTFDEMAEYNSFRGNEYRTYLATSK
                     DDLGNYVLKIDSLGRVATGVLPGDDKRNFHDGDETTKLNTDFPLLTDPEGLDALANNE
                     KLDPTDILAPKVSDKTRVYKGGSFKDRAYWLNPSTRRALDQDKSANDIGFRCAMSMIG
                     DIRSQSGKKK"
     gene            7884..9281
                     /gene="asnS"
                     /locus_tag="PKH68_05880"
     CDS             7884..9281
                     /gene="asnS"
                     /locus_tag="PKH68_05880"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNX12501.1"
                     /translation="MQRTKIVDALKRTDYGADVNIKGWVRTRRGNKNVSFIALNDGST
                     IHNIQIVVDLAKLDEEKLKPVTTGACISATGKLVESQGKGQTVEIQADDIVIYGSADP
                     ETYPLQKKGHTLEFLREIAHLRPRTNTFGAVFRVRHHMAIAIHKFFHDRGFFYFHTPI
                     ITASDAEGAGQMFQVTTLDLENIKLTEDGKVDHSDDFFGKPANLTVSGQLEGELAAMA
                     LGAIYTFGPTFRAENSNTPRHLSEFWMIEPEVAFNEIEENMQLAQDFIQYCIRWALDN
                     CMDDLNFLAEMYDKELIERLRFVVDNDFVRLPYTEGVKILEEAVAKGYKFEYPVGWGT
                     DLQSEHERYLVEQHFKKPVILTDYPKEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGG
                     SQREEDFEKLRTRAASMGVPEKDIWWYLDTRRFGTAPHSGFGLGFERLLLFVTGMTNI
                     RDVIPFPRTPKNAEF"
     gene            9322..>9640
                     /locus_tag="PKH68_05885"
     CDS             9322..>9640
                     /locus_tag="PKH68_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNX12502.1"
                     /translation="MEKLKVKELDQVVVRFAGDSGDGMQLTGSIFSNISAIIGNEIST
                     FPDYPAEIRAPQGTLGGVSGFQVHIGSGKVYTPGDDADVLVAMNPAALKVNAKHVKKG
                     GVII"
BASE COUNT         2642 a         2094 c         2093 g         2811 t
ORIGIN      
        1 ttaagatatc ttgaaataaa attattgtta catacagatt gtaaattaat attacctgat
       61 gaaccattta atggtgtttc accgatttta gttggagaaa taaaaaaata atactgaaaa
      121 tgtctgaatt taaagtaata atactgactg accatgacta caggaatgta ttggatgtgg
      181 cgaatcgatt ttgtatgatt tatgacggag gaataaagag aattgacgac aaagacgaac
      241 ttgtaaggtg gggatatatt cttcgtctgt tggagtgacg ccctcgctaa aaatgggcga
      301 gggcaggttc ttgtttcgtt tatgaaaaca tttccacgtc ttcttttgta atcttatttc
      361 ctcccagaat gatgagccgt tccaccacgt tgcggagttc gcggatattt ccggtccatg
      421 atcgttcctg cagggcttta acggcttttg catcgaaatg tttgagctga atgccttgtt
      481 cggcacaaat cagttcgctg aaatgggtga tcagcagggg gatatcgtct tttcgttcgt
      541 cgagtgtagg tacatgaatg gggataacat tgaggcggtg gaaaaggtct tcgcggaagt
      601 tgccgagttc tatttctttt ttcaggtttt tgttggtggc agcgatgacg cgcacattta
      661 ccttgatgtt tttgtcgctt cctactcttg aaatttcact ttcttgtaaa gcccgtagca
      721 ctttagtctg agctgacagg ctcatatcgc caatttcatc cagaaatatg gtgccattgt
      781 ctgcttgttc aaatttaccg atacgctgtt ttactgctga agtgaaagag cctttttcat
      841 gtccgaataa ttcgctctca atcagttcgg aaggaatggc agcacagttt acttccacgt
      901 aaggtccctg aacccggttg ctgtattcgt aaagcagtcg tgcaaccact tccttgccgg
      961 tcccgttagc acctgtaatc aatacccgtg catcggtggg ggctactttg cggatcattt
     1021 cgcgtacttt ttcgatggaa ggcgaattac cgatcatttg cagttttttg gcaactttct
     1081 ttttcagtgt tttggtttcg gttaccaggt tatttttttc cagggcattg cgtaccgtca
     1141 ccagtaaacg gctcagatcg atgggctttt cgataaaatc aaaagctcct tttttgatac
     1201 agtccacggc tgtttcaata tttccatgac cggaaatcat gatgaccggc gcttccactt
     1261 ctttttccct gagttgttcg agcaattcaa taccatccat ttcaggcatt tttatgtctg
     1321 aaaaaataat atcaaaaaat tcattcagag cagtttcaag tccctgtttg ccgttttcgg
     1381 ccagggtaac tttatgcttt tcgaactcca gaatatcttt caatgtattc cggatactgc
     1441 gttcatcgtc aataaccaat atttttgcca taatgataag cccctaaatc ccctaaaggg
     1501 gacttgtcgg gtagctaaat taaattttat aaatcttaga ttaatattaa aatcaggttc
     1561 tttttttttg aattatttac cgaatacaca taatttactt gaattttgta agtccccctt
     1621 cgggggatta gggggctttc tcataaatca aacccaatat cccgcctgaa atattttccc
     1681 tcaaaatcga tcaatttcgc attcttgtat gaaagttcaa gtgcttcgtc tttgtttttt
     1741 ccatacgaac ttacggcaat cacacgtccg ccgttggtga gaatatttcc gtctttttcg
     1801 gtggtgcctg catggaaggc aatgctctcc ttcacatctt caagtccggt aatcactttt
     1861 cctttttcgt aagcttccgg atagccgccc gatacgagca tcactgtcac tgctgaacgc
     1921 tcatcgaact caactgtttt tttattcaga ttaccagttg caacaccttc gagcaagtca
     1981 accagatccg atttcagtct cagcatcacc acttcagttt ccggatcacc catccttgca
     2041 ttgtattcaa tcacataggg ttcgttattc actttaatca gaccgaagaa gataaaccct
     2101 ttataggtaa tttcctcgct tcggaggcca tctacagtag gtttgatgat gcgttcgtcc
     2161 actttttgca taaatgcttt atcggcaaaa ctcacaggtg aaacggcacc catacctccg
     2221 gtgttcagtc cggtatctcc ttcgccaatg cgtttatagt cctttgcttc gggcagaatt
     2281 ttataatttt tcccgtcagt gatgacaaac accgaacatt caatacccga aagaaattct
     2341 tcaataataa cggtttggct ggctttcccg aattttccgc ccagcatatt attcagttct
     2401 tcaattgctt catccaaatc agagataatc aatactcctt taccggccga caatccatcc
     2461 gctttcaata cataaggggc tttcaatttt tttagaaaat cgatacctgc attcagattt
     2521 tcggaagtga cagacaaata tcctgctgtc ggaatgttgt gacgaatcat gaactgtttc
     2581 gaaaaatcct tactgccttc caattgggca gccaagcggt taggacccac aatcggtata
     2641 gatttcaatt catcgtcagt ttcaaaaaaa tcaacaatac catttaccaa tggttcttcc
     2701 ggaccaacca ccaccattcc gatattattt tcgagcacaa cccgttttac agcctgaaaa
     2761 tcggagatat tgaccgcaat attctcgccg gttgaatttg ttccggcgtt tccgggagca
     2821 ataaaaagtt tcgtcagttt cgggctttgt gcaattttcc aggcaatggc atgttcgcgg
     2881 cctcctgatc ctaaaagtaa tatattcatt ggattaatct gtttttttta ttcattttat
     2941 tttattcttc ccaaaccagg tacgatgata ccagccagtg attgagtttg ttgttatcta
     3001 ttgcttgtgc ctgcggaatg ctgatggtca gtgtgtgttc accggctttt atatcgtcaa
     3061 gaggtataat cgcgggtggc acatcgcttc ccggacacca gttggagcgg gagagatccg
     3121 atgaagcaat cggttcttca atttcttttt cggctctgcc ttcttcgctg atgtatgcaa
     3181 ttctgcgttt tttcagccac acccccgatg tgggattgaa gcggcgaaag gaagcacaat
     3241 cgtttcgcca gggagtaaac cggtgaacga cagccccgtc aacggagagt atattttccc
     3301 gctgtacaaa ttcatcgccg cctgaatggc ctccatgacc ggtcacaatg tatttcagcc
     3361 tgatattttt agcttttacg ggaattgtaa aaggaaccgt tacatcccgg cgtgaaaata
     3421 tgtcgggata agtctgacca atataataaa cagtattaat cagaggcaat actttccgca
     3481 cgggcatcac atcctgtttt atggtgcttt caatgaaatt gatttccaga tttaccttat
     3541 aacctttatc agtccaggtg tcgatgtaaa tgccgatgta tgcttcgttt tgcagtagtg
     3601 ggtacaggtc ggtaatatcc tgctgccaaa ccgattcctg agcccagcca tcaatataaa
     3661 ccggtcgtct tttgtaggct gtggagtctt tatcggcact gaaataaccc actccgaacg
     3721 gggtcataaa ccgcatcagt tccacagtgg gcaaataatc ttttccggca gctacaccca
     3781 ccagtttttc cagtttggtt gagtcgattt gcggatattg atgttcacct ttggcaatgg
     3841 tcatcatatt tatcattgac tctttcggga taataaaaca agaaccggtt ttgtcccacg
     3901 gatcaccatt ggaagtgagt ttcagtttgg ctgtgacacg cacatttcgt ttgtaatcag
     3961 ggattttgat tttttttatc gcaatgcgtc cgtttgccag acaaatgatg ctgtcggagt
     4021 tatttaatcc atccggaaat ttagaaggat taaatacaat attcaaatca gaaaacacac
     4081 gggcttgaaa gttgccgcgt gcaggagccg ttttggcatt gacggcctgt attccgatga
     4141 gcagcatcgc aacgtaaata attcgtttca tacgtttttt gctttgttgt attcggttat
     4201 ttcttcggtt aacacatgtt ccagctcttc aggtttgccg ttgatggtga gtttaccgtt
     4261 gatagcccag gaaatatgtc cggtttcttc ggatatgaca atggcaatgg catccgagat
     4321 ttccgaaatt ccgagtgccg agcggtgtct cagtcccagc cttttgggta tgctttggtt
     4381 tcgcgatacc ggaagaatgc aggcggctgc tctcaatcgg cggttagaaa tgataagggc
     4441 gccatcgtgc aaagggctgt ttttgaaaaa gatattttct atcagacggg aactgatacg
     4501 tgcatcaata atttcggccg attgggtata gaattccagg tcgttggtgc gggtgacgat
     4561 gattaatcct ccggttgccg ttttcgacaa gttacgacaa gctattacaa tctgtatcag
     4621 gtcgtaatcg gtgcggattt gttcgttttc agagcccttc agaaattttt tgaagtagtt
     4681 aagtattccc cagtttttgc gcgatccgag ccgtgagaag aaccggcgta tctcttcctg
     4741 aaaaagtaca atcagggcaa atgctcccac attcaccagt ccgtccagta ttacgcccag
     4801 gagttccatg cgcagtactt tggacactac gaaccagatg atgataaaag tcattattcc
     4861 caaaaacaca ttcagggctc cggatcgttt cagcagtttg aacgctccat agagcagaaa
     4921 tgccgttagg ataatatcaa tgatgtcttt aacgccgata ttaaaaagca ttttgttcgg
     4981 ttttttgaat tataatctgg aattaaaaat cttattttga tgattggaat tatgtacaga
     5041 ctacgccctc gttacttatt cacaggcggt tttgtatttt tcaaatattt ttatcgtttc
     5101 tacagcttcc tttacatcgt gaacccggag aatatctgcg ccgttcagca gtcccagcat
     5161 gttgagtgcg gtagtgccgt tgagtgcttc ttccggagtg gtgtccagta gtttatatat
     5221 cattgatttt ctggatattc catacagtat tggcaaattc agttctttga aataactcat
     5281 tttagccagc agttcgtaat tttggtcgag tgttttggca aaaccaaatc ccgggtcaat
     5341 gataatatct ttcacgccca gatggacgag ttgagccacc cttttttcga aaaaatgaag
     5401 tatttcagac atcatattgt cgtaatgtgt cagttgctgc atggtctgag gagtgcccga
     5461 tgtgtgcatg agaatataag aggtattgag acttgccacg gtttcaaaca tcaaattatc
     5521 cagcgtaccg ccgcttatgt cgttaattat gtccacacca catacttcca ctccccatcg
     5581 tgccactccc gaccggaatg tatccagcga aatcacagca tcgggaaaat gcttacgaat
     5641 agctttaagt ggcgaccgca gccgttccag ctcctcttct tcggtaacat gaggggctga
     5701 cgggcgggtg gaataagcgc caacatcaat aatggaagcc ccctgggcaa gtgctttttc
     5761 tacccggtta agtacatctt tgtccgaaaa taagcggctt ccactgaaaa atgaatcagg
     5821 cgtaatgttg gctatcgcca tcaccgccgg tttcgacaaa tcgaacaact tgccgttcag
     5881 attcagctgg aagggaattt tattcatctt tttttgcaca aattatatct ctgttacggg
     5941 catcaaaagc agttattttc cacaaaagta aaaaaataaa cgcttaaatt tatggtttta
     6001 acagatttat tgataatttt acctcaaaat atacgtttat accccaaaat gagttatata
     6061 tttacgctgt ggccaaaact tctatttttt ttagcataaa aggcacattc ataaaaaaaa
     6121 gtactaattt tgcagtcagc ttaaaattat acaaaagaac taattatcat tgttttatat
     6181 gaaattgaat aacttagcta catttctgac acttgcattt gctgctgtgg ttgttgcttc
     6241 ctgcggttca aaatcgggag ccagttccgg atttggatcc ggtaagaaat cggcaatatc
     6301 aaataccacc ggttggcgct acaacgacga aaaggcgacc gggtttggtg taaaaaagaa
     6361 ctacaagcct caggttccaa ccggcatgat tgctattgaa gggggtacgt ttaccatcgg
     6421 cgagaaaggc gaagtggtca ccggcgaacg caataacctg cgccgaagga ttaccgtcat
     6481 gtcgttctac atggatcagt acgaaatcag caacgttaac tggcgtgaat atacgcactg
     6541 gatgaagacc gtatttcagc agacagcacc ccggttggtg gaaaggacac aacccaatgc
     6601 cgaagtatgg cgcgaaccgc tggcatacaa cgaaccgctc cttgagcact acttcaccca
     6661 tccggcattc gatgagtatc ccattgtggg tgttacctgg gaacaggcta tggattactg
     6721 ccagtggcgt accgatcggg taaacgaatt ggcgctgatt aatgccggca tcattgacgc
     6781 accggatttt tcttcgttga agaaaataac aaatgccgac tcaattgcaa ataattttgt
     6841 gttcaacaca cagaaataca tttaccatag aaattataat cccaaagccg gtaaaaaatc
     6901 aatgaaagat atatacggcc aaacacgcaa agccgatatg tcagacggca ttatgacctc
     6961 caattaccga ctcccgactg aagccgaatg ggaatttgca gcttatgcta tcaggtccga
     7021 caaaaaagac ggattggtga aagagggaag agtgtatccc tggaacggaa gccagttgcg
     7081 caacagtaaa aaaggaaaat acgaaggaga aatgcaggct aactttgtgc gtggccgggg
     7141 cgatatgatg ggaacatccg gaagcctcaa cgacaaagct gccattacag ctcccgtgac
     7201 tgcctatttc cccaatgaat ttggtctgta taatatggca ggtaacgtca atgaatgggt
     7261 gctggatgtg taccgttcca caacattcga cgaaatggcc gaatataatt catttcgagg
     7321 caatgaatat cgcacctacc tggccacctc aaaagatgat ctgggaaatt atgtgctgaa
     7381 aatagactca ctggggcgtg tagctacagg tgtattgccc ggtgacgaca aacggaattt
     7441 tcacgatggc gacgaaacca ccaaactgaa taccgacttc cccctgttga ccgatcccga
     7501 agggcttgac gcattggcca ataatgagaa attagaccca actgatattc tggctccaaa
     7561 agtctctgac aaaacacgtg tttacaaagg aggttccttc aaagaccggg cttactggct
     7621 taatccatct acccgacgtg cactggatca ggacaaatcg gccaatgata tcggattccg
     7681 ttgtgccatg agcatgattg gcgacattag aagtcagtca gggaaaaaga aataaaaatt
     7741 attatcagaa taataagaag aagcacctgt tcggttggac aggtgctttt tgtttatttt
     7801 tttcaagctt ttctgaacta aatgtattac ttttgcattt taaatttctt acttaactta
     7861 cacgaaatta taactatagt tttatgcaac gaacaaaaat tgtggacgcc ctcaaaagga
     7921 cggattacgg agcagatgta aatattaaag gctgggttcg gacccgccgc ggtaacaaaa
     7981 atgtcagctt tattgccctg aatgatggat ctaccataca taatattcag attgttgttg
     8041 atctggccaa actggatgag gaaaaactta aaccggtgac taccggtgca tgcatcagcg
     8101 ccaccggcaa gttagtcgaa tcgcagggta aaggacaaac ggttgaaatt caggcggatg
     8161 atatcgttat atacggcagc gccgaccccg aaacatatcc attgcagaaa aaaggtcata
     8221 cactggaatt tttacgtgaa atagcgcatt tgcgcccccg taccaatacg tttggagctg
     8281 tgttccgcgt tcgtcaccac atggccattg ctattcataa attctttcac gaccgtggat
     8341 ttttctattt ccacacgccc attatcaccg cttcggatgc cgaaggtgcc ggacaaatgt
     8401 ttcaggtaac tacgcttgac ctggaaaata tcaagctgac tgaggatgga aaagtggatc
     8461 acagcgacga tttctttggt aaaccggcca atctcaccgt ttcgggacag cttgaaggcg
     8521 aacttgccgc tatggcgctg ggagcaattt atacattcgg accaacattc cgcgctgaaa
     8581 attccaacac accgcgtcac ctgtcggaat tctggatgat tgaacccgaa gtggcattca
     8641 acgaaataga agaaaacatg cagctggcgc aggactttat tcagtactgc atccgctggg
     8701 cgctcgataa ctgcatggat gatctcaact tcctggccga aatgtatgat aaggaactga
     8761 ttgaaagact gcggtttgtg gtggataatg attttgtgcg gctgccttac accgaaggag
     8821 tgaaaattct tgaagaagct gtagcaaagg gatataagtt tgaatatccc gtaggttggg
     8881 gaactgattt gcagtcggaa cacgaacgat atctggtaga acagcatttc aaaaaaccgg
     8941 taattctgac cgattatcca aaggaaatca aggcattcta catgaaacaa aacgatgacg
     9001 gcaaaaccgt acgggccatg gatgtacttt tcccgaaaat cggagaaata atcggtggat
     9061 cgcaacgtga ggaagacttc gaaaaactaa gaacacgtgc cgcctccatg ggagttcccg
     9121 aaaaagatat ctggtggtac ctggatacac gccgttttgg cactgcacca cattccggtt
     9181 tcggtctggg cttcgaacgg ttgctgctgt tcgttaccgg tatgaccaat atccgcgatg
     9241 tgataccgtt cccgcgtact ccaaaaaatg ctgaatttta aaaaatgaga gattattccg
     9301 aaaaatctga atttatataa tatggaaaaa ctaaaagtaa aagaattgga tcaggttgtg
     9361 gttcgctttg ccggtgactc cggtgacgga atgcaattga caggctctat tttttcgaat
     9421 atttcggcca ttatcggcaa tgaaatttct actttccccg attatcctgc cgaaattcgt
     9481 gcccctcagg gtacattagg tggagtttcc gggtttcagg tacacattgg ttccggtaag
     9541 gtctatactc cgggcgacga tgccgatgtg ctcgtggcca tgaaccccgc cgcactcaaa
     9601 gtaaatgcca aacatgtaaa aaaaggcggt gtaatcattt
//
LOCUS       DAOPZK010000075         9198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_8878_length_9198_cov_23.526742, whole genome shotgun sequence.
ACCESSION   DAOPZK010000075 DAOPZK010000000
VERSION     DAOPZK010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8878_length_9198_cov_23.526742"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..369)
                     /locus_tag="PKH68_05890"
     CDS             complement(22..369)
                     /locus_tag="PKH68_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX12503.1"
                     /translation="MRNDKQNPIVDKSFQFAVEIVEFCDLLYENKKYVIAQQLLKSGT
                     SIGANIREAQNAEIKADFIHKMKIAAKEADETEYWLLICKYSNSYPTNLDLIEKVNEL
                     LRILSKIISSSKS"
     gene            complement(480..908)
                     /locus_tag="PKH68_05895"
     CDS             complement(480..908)
                     /locus_tag="PKH68_05895"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNX12504.1"
                     /translation="MKKLISLSVVMLFVLSFFSVNSFAADKKTKTAVSKAPKVEVYYF
                     HFTRRCVTCNAVENVTKEAIAEMYPQQLKAGKITFTGVNLDEEGSKAIAEKCKAEGQA
                     LLVISGGKRIDLTDKGFMYARSKPENLKKELNNTIDPLLK"
     gene            complement(905..1153)
                     /locus_tag="PKH68_05900"
     CDS             complement(905..1153)
                     /locus_tag="PKH68_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNX12505.1"
                     /translation="MEIKILGTGCPKCKSLEKATREVVAQNNIDATITKVEDIMEIMN
                     FNVMTTPALVVNGKVVVKGRIPSNDELKQILTNKIYII"
     assembly_gap    1306..1405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1406..1724)
                     /locus_tag="PKH68_05905"
     CDS             complement(<1406..1724)
                     /locus_tag="PKH68_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNX12506.1"
                     /translation="MNSFKNKDYTIDNEQLARFAKAMGHPARITILQFLASMDSCYFG
                     DIHNELPIAKATVSQHLKELKDAGLIQGEIETPKVKYCINRDNWEKAQKYFETFFALR
                     NEKT"
     gene            2081..3805
                     /gene="recJ"
                     /locus_tag="PKH68_05910"
     CDS             2081..3805
                     /gene="recJ"
                     /locus_tag="PKH68_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNX12507.1"
                     /translation="MINKWIYSELTEEQNAIKNDLANELSISPILAQLLVQRDITTYD
                     DARSFFRPDLGDLHDPFLMKDMDKAVERLTRAMQKNEKILVYGDYDVDGTTSVSLVYK
                     FLLQFYSNIDFYIPDRYNEGYGISVKGINHAAQNDFKLVIALDCGIKAVEKVKYATSL
                     GVDFIICDHHNPDDVLPAAIAVLDAKREDCNYPYKHLSGCGVGFKLMQAFAIVNEIDF
                     QNLIPLLDLVALSIASDIVPITGENRILAFYGIKQINTNPSTGIKAILEVCGLKDKEI
                     SISDIVFKIGPRINASGRMKLATEAVELLVARDYGFAYKRSSTINVYNNDRKDLDKSI
                     TDEAIAMIRADQSYVDRKSIVVYKPDWHKGVIGIVASRLSEEYYKPTIVLTKSNGFAS
                     GSARSVSGFDLYKAIETCRDLLENFGGHMYAAGLTMKEENVEEFTRRFEQYVYDNILE
                     DQTYPQIEIDAVLQFSDITPKFFRVLKQFGPFGPGNMKPVFESKKVYDYGSSRLVGKE
                     QEHLKLELIDSSSENVMSGIAFRMFEYNDHLKALNPLDICYTLEENTFNGNTNIQLMI
                     RDIRKVEK"
     gene            complement(3881..4459)
                     /locus_tag="PKH68_05915"
     CDS             complement(3881..4459)
                     /locus_tag="PKH68_05915"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNX12508.1"
                     /translation="MIYSEQNHPNQVKKGSIEVICGSMFSGKTEELIRRLKRAKFAKQ
                     RVEIFKPKIDTRYSEEDVVSHDRTAIRSTPVESSGSILLMSSEVDVIGIDEAQFFDEG
                     LVEVCKTLANGGIRVIVAGLDMDFRCVPFGPMPALMAVADDVFKVHAICVRCGALAYV
                     SHRLVAGDKQVLLGEMTEYEPICRDCYNELKK"
     gene            4616..5464
                     /locus_tag="PKH68_05920"
     CDS             4616..5464
                     /locus_tag="PKH68_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12509.1"
                     /translation="MNKDMIVLLLKRDIQELNLLTDGFEKMSEFPEPLLILAKQKAEN
                     IHQSLCELSDTSVQSEWGAIDYQSFGEDTSEVSVMNNFDAPLDAADEILTEKGQTEPV
                     TEQSFTIADEPETLTYEETQTEPVIVSPEETEVLPDMSETVETELTPTEANVEETPEV
                     VNIADIEPEIQIENLPHPEPVIIPNKSSVLVDELMADRDLSLGENLANLKINDIRQAI
                     NIGDRFRFQRELFNGNGEVMNKTIAYLNQLQKYEEAMSFLKAKFNWTADNNHADDFMQ
                     IVKRRY"
     gene            5716..6387
                     /gene="rsmI"
                     /locus_tag="PKH68_05925"
     CDS             5716..6387
                     /gene="rsmI"
                     /locus_tag="PKH68_05925"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNX12510.1"
                     /translation="MKLFVVPTPIGNLEDMTYRAVKVLREVDLILAEDTRTSGFLLKH
                     YDIKTPMHSHHKFNEHKTVQNVVERIKAGQNVALISDAGTPAISDPGFMLVRACVENE
                     VEVECLPGATAFVPALVTSGLPNDRFCFEGFLPQKKGRQTKIHALAAETRTMIFYESP
                     FRVVKTLTQLAEVFGADRKASVSREISKIYEETVRGTLTELAEHFTLHNPKGEFVIIV
                     AGFEE"
     gene            6496..7371
                     /locus_tag="PKH68_05930"
     CDS             6496..7371
                     /locus_tag="PKH68_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12511.1"
                     /translation="MKSYILYFAAALFLISSCGIKSGREVDQLKAQNDSLMNSKLQLE
                     KEVNEYFSAMNEVQENIEKIKNAQNVLTVEPLSENTPEDVRTKVVEDMNYINDLIKTN
                     QDELDRLRARLKKSSFKLLDVEKTLASLTKQLNQESAKVAALQAQIQQKDSVITRLGT
                     TVDELGKNVENLNVQNQEKETKIQEQDAALHSAWYAIGSRKELKENKIISSDGIFSAK
                     KVLQSDFNKNYFVKVDARNTRKIPLYSTSKAAILTSHPKASYTLEKENDNYVLVIVDP
                     AEFWSVSKYLVVEVD"
     gene            7544..8245
                     /locus_tag="PKH68_05935"
     CDS             7544..8245
                     /locus_tag="PKH68_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNX12512.1"
                     /translation="MKSRYNYIFLDLDDTIWDFHANAKASLHDVYFDRKLNEHYPDFE
                     HFFHLYARRNLELWSQYGQGLITKDYLIVERFRHLLSHAGIQAHEMALNMNTDFLDIL
                     STKTILMPYAGELLEYCTARHLPMTIISNGFTEVQYRKLRNSNIEQYFTHVVLSEAVG
                     ALKPSREIFEYALKLNNARADETLMIGDSFDADILGAVGAGIDAVYLNNTRRDIEIPE
                     KVTQISSLKEVFGLI"
     gene            8539..8745
                     /locus_tag="PKH68_05940"
                     /pseudo
     CDS             8539..8745
                     /locus_tag="PKH68_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            8740..9000
                     /locus_tag="PKH68_05945"
     CDS             8740..9000
                     /locus_tag="PKH68_05945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX12513.1"
                     /translation="MRYQQEDNGKKGRFILYFNDEFAGEITYTWAGEETIIIDHTGVE
                     EKFGGKGFGKALLQQVLLFTRGKDLKIIPLCPFVKRQLEKNN"
BASE COUNT         2866 a         1977 c         1814 g         2441 t
ORIGIN      
        1 aatttgaaaa tgaataggtt gttaagattt ggatgaacta ataatttttg atagaattct
       61 tagtaattcg tttaccttct cgattaaatc cagatttgtt gggtaggaat ttgaatattt
      121 gcaaattaac aaccaatact cggtttcatc tgcctctttt gcagcaattt tcattttatg
      181 aataaaatcc gccttaattt cagcattttg tgcttcacgt atgttagcac caattgacgt
      241 tcctgatttc aacaattgct gtgctattac atattttttg ttctcgtaga gcaaatcaca
      301 aaattcaact atctcaactg caaattgaaa tgatttatct acaattggat tctgtttgtc
      361 gtttcgcata cttattattc taaaattttc tcatttccaa ttcctgaact catctttata
      421 ccataaatac attttcaaat tagcacattt tcaaattata aaattttcaa atcttcaaat
      481 tatttcaaaa gcggatctat cgtattattc aattcctttt tcaagttctc cggcttgcta
      541 cgggcataca taaaaccttt gtcagtaagg tcaatacgtt taccaccact aataaccaat
      601 agtgcttgtc cttctgcttt acatttttcg gctattgctt tactgccttc ttcatccaaa
      661 ttcactcctg taaaagtaat ttttcccgct tttaactgtt gaggatacat ttcggctatc
      721 gcctcttttg taacattctc tacggcatta catgttacac accggcgggt aaaatggaag
      781 taatatactt caaccttagg ggctttggaa actgctgtct ttgttttttt atcagcagca
      841 aaactattaa ccgaaaagaa agacaataca aaaagcatta ctacagataa tgaaattagc
      901 tttttcatat aatatagatt ttatttgtta gaatttgttt taattcatcg ttagaaggta
      961 tacgtccttt taccactact tttccattaa caaccagggc cggtgtggtc atgacgttga
     1021 agttcattat ttccattata tcttccactt tggtaattgt agcatcaata ttgttttgag
     1081 ctactacctc acgtgtggct ttttcaagtg acttgcactt tggacagccg gttcccagaa
     1141 tttttatttc cataaagata aattagtacc cccaacctcc cgaaacaagt tcgggacaag
     1201 ctctaaaggg gtgtaagaat ggttagtatt tataatttgt taagtattct gtatccactt
     1261 tgatatctta aaatacaaaa aagaacttgc ttaattcccc tttagnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnttgtt ttttcatttc tcaatgcaaa aaatgtttcg
     1441 aaatatttct gtgctttttc ccaattatca cggttaatgc aatacttcac cttgggtgtt
     1501 tctatttcgc cctgaataag tcctgcatct tttaattctt tcaaatgttg tgatacagtg
     1561 gctttggcta tgggcaattc gttgtgtatg tcgccaaagt agcaactatc catcgaagcc
     1621 agaaattgta aaatggtaat gcgtgccggg tgccccattg ctttagcgaa acgagcaagc
     1681 tgttcgttgt caatggtgta atccttattt ttgaagctat tcatatagat ttgtattgtt
     1741 tctttgtagt tcgcaaaact acgaacaata tttgaattct caactttttt atttgaatat
     1801 tttaatttgc tttaaattat tgatccaatg attttattcc gtcattattc ctgaaacgaa
     1861 ccataaataa gaataaatcc tttgtaattt tgtcattttc gaaaaaaaac actattttta
     1921 cagattataa ccgaaaaagt gtgaacgaaa tgccccgatg agttgtttat ggaaaagttg
     1981 agatgatata acagagttga ttgtgtggat gatataacag agttgattat tagtgaaata
     2041 cagcggtatt tcacccctgc ccgaaaaaat aatacacaaa atgataaaca aatggattta
     2101 ttcggaacta accgaagaac aaaatgcaat aaaaaatgat cttgccaatg aattaagtat
     2161 tagtcccata ttagcacaac ttctggtgca acgcgacatc accacatacg atgacgcacg
     2221 tagttttttc cggcccgatc tgggtgactt gcatgatcca tttctgatga aagacatgga
     2281 taaagcggtg gaaagactaa ccagagcgat gcaaaaaaac gaaaaaatac ttgtttacgg
     2341 cgattatgat gtggatggaa ctacttccgt atcactggtt tataaattcc tccttcagtt
     2401 ctattccaat atcgatttct acattcccga ccgctacaat gagggatacg gaatatcggt
     2461 gaaaggaatt aaccatgcag cccaaaacga ctttaaattg gttatagcgc tcgattgtgg
     2521 tattaaagcc gttgaaaaag tgaaatatgc cacctcattg ggcgttgact tcattatctg
     2581 tgatcaccac aatcccgacg acgttcttcc ggctgccatt gccgtgctcg atgctaaacg
     2641 cgaagactgc aactatccct acaaacacct ttcgggctgc ggagtaggct tcaaactgat
     2701 gcaggcattt gccattgtca acgagattga ttttcaaaat ctcattcccc tgctcgattt
     2761 ggtcgccctg agcattgcat ctgacattgt tccaataact ggtgaaaacc gcattcttgc
     2821 cttttacggg ataaaacaaa tcaatactaa ccccagcacg ggcatcaaag ccattctgga
     2881 agtgtgcgga ctgaaagaca aagaaatctc catcagtgat atagttttca aaatcggacc
     2941 gcgcatcaat gcttcgggac gtatgaaact ggcaaccgaa gcggtcgaac tgctcgttgc
     3001 acgtgattat ggctttgctt acaagcgcag cagcaccatc aatgtatata acaacgaccg
     3061 taaggatctg gacaaatcca ttaccgacga agcaatagct atgattcggg ccgatcaaag
     3121 ctatgtcgac cgaaaatcaa tcgtggtgta caaacccgac tggcacaaag gagttatcgg
     3181 tatagtggct tcacggcttt cggaagagta ctacaaacca accattgtac tcaccaaatc
     3241 gaacggtttc gcttcaggtt cagcccgatc ggtttcgggt ttcgatcttt acaaagctat
     3301 agaaacctgt cgtgacctgc tcgaaaactt tggcggacac atgtatgccg ccggactgac
     3361 catgaaagag gaaaatgtgg aggaatttac ccgtcggttt gaacaatacg tgtacgataa
     3421 tattctggaa gatcagacct atccgcaaat cgagattgac gcggtgcttc agttcagtga
     3481 cattacaccc aagtttttca gggtactcaa acaatttggt cccttcggtc ccgggaatat
     3541 gaaacccgtg tttgaatcga aaaaagtata tgactacggc agcagccggc tggtgggcaa
     3601 agaacaggaa cacctcaagc tggaactgat cgacagcagc tcggagaatg tgatgagtgg
     3661 tattgctttt cgcatgttcg aatacaacga ccacctgaaa gcgcttaacc cgctggatat
     3721 ctgctacacc ctcgaggaaa acactttcaa cggaaatacg aacatccagc tgatgatacg
     3781 tgatatccgg aaagtggaga aatagaggtc cacagaaata taaaacaaaa gcgtcggatt
     3841 aatctgttaa tccgacgctt ttgttttctt aaaaatgctt ttactttttc agttcgttgt
     3901 aacaatcccg gcaaataggt tcgtattcgg tcatttcgcc cagcagcacc tgcttgtcgc
     3961 ctgccaccaa ccgatgggaa acataagcca gcgcgccgca acgcacacaa atggcatgaa
     4021 ccttaaaaac atcgtcggcc acagccatga gagcgggcat gggtccaaag ggcacacacc
     4081 tgaaatccat atccagcccc gctacaatca cccggatacc accattggca agggttttgc
     4141 agacttccac cagaccttcg tcgaaaaact gagcttcgtc aatgccgatt acatcgactt
     4201 cgctggacat aagcaggata cttcccgacg actccacagg ggtggaacga atagccgtac
     4261 ggtcgtgcga taccacatct tcttccgagt atcgggtgtc aatcttgggt ttgaaaatct
     4321 ccacccgttg ttttgcaaac ttcgcacgtt tcagccggcg gatcagttcc tccgttttgc
     4381 ccgaaaacat cgaaccgcat attacctcta tactaccttt tttcacctga ttgggatgat
     4441 tctgctccga atatatcatt actttttatc gaaaaatatg ttttacctgt gtactttttc
     4501 aattcattaa ttcgtatctt tacaccgttt tattttgatg taactatcct tcatgaataa
     4561 tttccacaaa aataaaaaaa atacttatac cgaaactgtt tttaggaaaa aagatatgaa
     4621 caaagacatg attgttctct tgctcaagag agatattcag gaactgaacc tgctaaccga
     4681 tggatttgaa aaaatgagcg aatttcccga acccttactt atactggcca aacagaaagc
     4741 cgaaaacata catcaaagcc tctgcgaact gagcgataca agcgtccaat ccgagtgggg
     4801 ggcgattgat tatcagtcat tcggcgaaga taccagcgaa gtaagtgtta tgaacaattt
     4861 tgatgctccg cttgatgctg ccgatgagat acttactgag aaaggacaaa ctgaaccggt
     4921 aactgagcaa tcctttacaa tcgccgacga accggaaacc ctcacttacg aagaaacaca
     4981 aaccgaaccg gtcatcgtgt cgcctgaaga gacggaagtt ctgcctgaca tgagtgaaac
     5041 tgttgaaacg gaattgacac caacagaggc aaacgtagaa gaaactcctg aagtggttaa
     5101 cattgccgac atagaacctg aaatacagat tgaaaacctg cctcaccccg aacctgtcat
     5161 catcccgaac aaatcatcag ttctggtgga tgagctgatg gcagatcgcg acttgtcact
     5221 gggcgaaaac ctggccaacc tgaaaataaa tgatattcgt caggcaatca acatcggcga
     5281 tcgcttccgt tttcaacgcg aactcttcaa cggcaacggt gaagtgatga ataaaaccat
     5341 tgcctacctc aatcagctgc aaaaatacga agaggccatg tcgtttctga aagcaaaatt
     5401 taactggaca gccgataata atcatgcgga tgattttatg cagattgtga aaagaagata
     5461 ttgagccccc ctgcccccct caagggggtt caagtgaaaa cagttacaag ttataaagga
     5521 ataagtgtgt catatttttt ttatttatgt aactcaatgg tgatataact tttcaaatgc
     5581 tgagataatt acaaaacgcg ataattatcg gagaatacac acattggcaa aataaaagca
     5641 ttagatataa aaaggaaatg attaatagcc agccaattcc ccctttaggg ggttaggggg
     5701 caaatatata atcacatgaa attattcgta gtacctaccc caatcggtaa cctggaagat
     5761 atgacctatc gcgccgtgaa agtgcttcgc gaagtggatt tgatactggc cgaagacacg
     5821 cgtacgagcg gttttctgct caagcattac gacataaaaa ctccgatgca ctcacaccac
     5881 aagttcaacg aacacaaaac ggtgcaaaac gtggtggaac gcattaaagc cggacagaat
     5941 gtggcgctga tatccgatgc gggcactccg gccatatccg atccgggttt tatgctggtg
     6001 agagcctgcg tggaaaatga ggtggaagtg gaatgtctgc cgggtgccac tgcattcgtg
     6061 ccggcgctgg tcacttcggg tttgcccaac gaccgctttt gctttgaagg atttctgccc
     6121 caaaaaaaag gccgtcagac caaaatacat gccctcgctg ccgaaacaag aaccatgatt
     6181 ttctacgaat cgcctttccg ggtggtgaaa actctcacgc agcttgccga agtattcggc
     6241 gccgaccgga aagcttccgt atcacgtgaa atttcgaaaa tctacgagga aacggtacgg
     6301 ggtacgttga ccgaactggc tgagcacttt accctccaca atcccaaggg ggaatttgtg
     6361 attattgttg cagggtttga agaatagcac tacctttgca cacaggtaaa tcaccctttg
     6421 ccagaacaat ttaacacact gtataacaaa caaataatta atccgttttg ttagtaaata
     6481 acaaaataac acacgatgaa atcttacatt ttatatttcg ccgcagctct gtttttaatc
     6541 tcctcatgcg gaattaaaag cggtcgcgaa gttgatcaac tgaaagccca aaacgactcg
     6601 ttgatgaact ctaaacttca gcttgaaaaa gaggttaatg aatacttctc ggccatgaat
     6661 gaagttcagg aaaatattga aaaaatcaaa aacgcccaga atgttcttac ggtggagccg
     6721 ctcagcgaaa acacccctga agatgtacgt accaaagtgg tggaggacat gaattacatc
     6781 aacgacctga taaaaaccaa tcaggatgaa ctcgaccggc ttcgtgcgag actcaaaaaa
     6841 agctctttca aactgttgga cgtagaaaaa acactggcct cgctgaccaa acagctaaac
     6901 caggaatcgg ccaaggtggc tgctcttcag gcacagatac agcagaagga ctcggtgatc
     6961 accagactgg gtacaacagt ggatgaactg ggcaaaaatg tggaaaacct caacgtacaa
     7021 aaccaggaaa aagaaaccaa aatacaggaa caggatgctg cacttcattc ggcctggtac
     7081 gctatcggct cccgaaagga actgaaagag aataaaatca tatcgtcgga tggaatcttc
     7141 agtgccaaaa aagtgctgca gtccgatttt aacaaaaact attttgtaaa agtggatgcc
     7201 cgcaatacgc gaaaaatccc cctctactcc acctccaaag cagccatact cacctcgcac
     7261 ccgaaagcaa gctacacact ggaaaaagaa aacgacaatt acgtactggt tatagttgat
     7321 ccggccgaat tctggagcgt aagcaaatac ctggtagtgg aagtggattg atagcagaca
     7381 aaagactttt agagcaaaga gcaaagactt ttagaacaaa gagtaaagaa caaagacaaa
     7441 agacttttag aacaaagagc aaagacaaaa gacaaaagac aaaaaaagag acacggaaca
     7501 ctaaacgcta aaagctaaac actaaacgct aaagcaaagg gtattgaaaa gcaggtacaa
     7561 ctatattttt ctcgatttgg atgataccat ctgggatttt catgccaatg ccaaagcatc
     7621 gcttcacgat gtttatttcg accgcaaact aaacgaacat tatcccgact tcgagcattt
     7681 ttttcacctc tacgcccgtc gaaatctgga gttgtggtca cagtatggac aagggctgat
     7741 taccaaggat tatctgattg tagagcgatt ccgtcatctg ctcagccatg caggcattca
     7801 agcgcacgaa atggctttga atatgaacac ggatttcctg gatatccttt ctaccaaaac
     7861 catactcatg ccttacgccg gcgaattgct ggaatactgc actgcccgtc acttgcccat
     7921 gaccataatc tccaatggtt ttaccgaagt tcagtaccgt aaactccgca actctaacat
     7981 cgaacagtat ttcacccatg tagtgctgtc ggaagcggtt ggcgctctca aacccagcag
     8041 ggaaatattc gaatatgccc tcaaactcaa caatgcccgt gcagatgaaa cattgatgat
     8101 cggcgacagc tttgatgccg atatactcgg tgcggtcgga gccggcattg atgccgtgta
     8161 cctcaacaat acccgcagag acatagaaat acccgaaaag gtaacccaaa tcagcagtct
     8221 gaaagaagtc ttcggattga tttaagcaga gaacttttct tgtcgttaaa acacacctac
     8281 cctgtcgcat agtgtggaga tgtaaaaaat tgattctgat ggctacatct ccctgaagca
     8341 gaggtggaaa tgatgtacgg gaaagggcaa aaaccagatt tccccgaaac agtgcaaccg
     8401 tacaggtgac attttcgtta ccggattttg aagaatattt cagcgactaa aaaaaaatta
     8461 ttccccgtga taaacaaccg gagttgctaa ctgttttatc attaataata aacccatcat
     8521 tcaaaaaaaa taaattccat gacaaaagag tattccaacg gtgaagtaac cgtagtatgg
     8581 accccggata aatgcattca ttcgggcatt tgtgtcaaaa cattaccaaa ggtatataat
     8641 acaagcgaaa gaccctgggt gaaacctgaa aatgccacat cagaagaact ggtagtgcag
     8701 tgtgccatgt gtccttcggg cgcattatcc atcaaaacca tccgctacca acaggaagat
     8761 aacgggaaaa aaggtcggtt tatcctctat ttcaacgacg aattcgccgg agaaataacc
     8821 tacacatggg ccggtgagga aacaataatt atagaccaca ctggagtgga agaaaaattt
     8881 ggagggaaag gatttggcaa agcattattg cagcaggtgc tactgtttac ccgtggcaag
     8941 gacttgaaaa taataccact ttgtccgttt gtaaaaagac aactggaaaa aaacaactaa
     9001 ctcctgtgcg cgttacctta actgttaagt tgcaattttc agcgatagga aatccaattg
     9061 ggagtgccac tccaagtggc gcccccaatt gcaacaattt ttccggtgta atctgacaga
     9121 aaaaagtgac aatattacaa caagaggctg tctcaaaagg gcagtttctt ttttttttct
     9181 tatgctgcaa tttcaaga
//
LOCUS       DAOPZK010000076         9144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_8951_length_9144_cov_24.877434, whole genome shotgun sequence.
ACCESSION   DAOPZK010000076 DAOPZK010000000
VERSION     DAOPZK010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8951_length_9144_cov_24.877434"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKH68_05950"
     CDS             complement(<1..608)
                     /locus_tag="PKH68_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNX12514.1"
                     /translation="MILDILLITLGFILLIKGADWLVNGASALAKKHNISDLVIGLTI
                     VAFGTSAPEMVVNVIASFDKHSDIVFGNIIGSNNFNLFVILGVVALIFPISVKSSMVR
                     KEIPISFVVAFILMLLSNAILGQKENVLSKWDAALLFGLFLVFLFFTFRQPNTDEVPE
                     LTTDIKPMSGFKIWGFIVVGLSLLIIGGRLVVNSAIAIAQDFG"
     gene            complement(620..1576)
                     /locus_tag="PKH68_05955"
     CDS             complement(620..1576)
                     /locus_tag="PKH68_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNX12515.1"
                     /translation="MKKMEKERAKRNRTLTLTDNDREELIKRVLIPKKNKPLTPDEIR
                     NKTILADLFDVIDYLPANSADLIIIDPPYNLTKNFHGFKFTSSTDDEYLAYLRSWFSK
                     IVSVLKPNGSLYLCGDWKCTAALQRVMSENLTILNRITWQREKGRAATHNWKNGMEDI
                     WYGVKNPKDYYFNVDAVKIKRRVLAPYRENGRPKDWEQTGDGKFRLTGPSNFWDDISV
                     PYWSMPENTDHPTQKPEKLIAKIILASCPPDGIVFDPFLGSGTTSVVAKKLGRNYIGI
                     EMNTEYALWAEKRLLKAEQSPAIQGYTNGVFWERNSGKQMLD"
     gene            1800..2270
                     /locus_tag="PKH68_05960"
     CDS             1800..2270
                     /locus_tag="PKH68_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNX12516.1"
                     /translation="MDQHQKFMRKAIGLSVQNLKKGGGPFGAVIVKDNKVIATGVNRV
                     TSHNDPTAHAEIVAIRSATRKLHSFNLQGCTIYTSCEPCPMCLAAIYWAHLDKIYYAN
                     TKTDAKNIGFDDSFIYDEIALDPDLRKIETTRLLSAEAITAFERWTEMDEKMEY"
     gene            complement(2352..3344)
                     /locus_tag="PKH68_05965"
     CDS             complement(2352..3344)
                     /locus_tag="PKH68_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX12517.1"
                     /translation="MAYTPSSDRYTHTVGYNYCGKSGLQLPKLSLGLWHNFGDVNDLT
                     EARKMLETAFDNGITHFDLANNYGTPFGSAEANFGKILKEDFASYRDELIISTKAGHT
                     MWDGPYGDWGSRKYLIASLNQSLKRMGLEYVDIFYSHRYDPNTPLEETMGALSDMVKQ
                     GKALYVGISKYPTDKARQAYRILAENGTPCLINQDRYNIFTREIEQTVLPLTKEKGIG
                     FIAFSPLAQGLLSNKYLNGIPENSRAAQSYGFLQKDQVTPELVKKLKKLNEFAKSRGQ
                     SLAQMALAWCLHDRQVTSVIVGTSSVKQLKDNIGALENTVFTDEEMWLIGEIAG"
     gene            complement(3410..3994)
                     /locus_tag="PKH68_05970"
     CDS             complement(3410..3994)
                     /locus_tag="PKH68_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNX12518.1"
                     /translation="MKKTGIIVIIALIAILFLWGMGGYNGLVNSQENVKSQWGNVENQ
                     YQRRADLIPNLVETVKGYATHEKSTFEEVIAARAKATQITVDPTNLTPEKLQEFQAAQ
                     GQLSSALGRLIAVAENYPDLKANQNFLELQAQLEGTENRISVERNKFNEMAKEYDVAI
                     RRFPKNIIAGIFNFNNIPYFAAEKGAEKAPSVKF"
     gene            complement(4051..>5362)
                     /locus_tag="PKH68_05975"
     CDS             complement(4051..>5362)
                     /locus_tag="PKH68_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNX12519.1"
                     /translation="LFVLLGLWLGTLYAQEYTVESVPNPITANAHHYVSNPDGILSES
                     TVNTLNAKLDSLSLNNGAQVAVVVLNSIGFDVIDNFAWSLFDKWRIGKEKEDNGLLVL
                     FVLDQKKVRFETGYGLEGVIPDAIAKRIQSTVMVPEFRNGNYDAGMLAGVNGIISAIN
                     KEPVSEETPAPAFNWNEVLPFALAGYILLALLTFLWTRSITSQTLRNNTLSTNLSRYK
                     VIRENNNSVASMVGLYLPVILLGLILFFASSLYLIFLLPVPLTVLPSYLYGRSMMTRV
                     RRAPIPCKACGSQMHILSEKEDDAHLHLSQKLEEELKAVDYDVFVCENCKEEAIFTLD
                     KKSQYETCPKCSTKAFILDSKRMVVAPTYVHAGTERLTYKCKFCGYQENNNRNIPRLK
                     RNNSGFFGGAVGGGMFSGGGGFGGGGGGSFGGGMSGGGGATSGW"
     assembly_gap    5363..5462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5573..6961)
                     /locus_tag="PKH68_05980"
     CDS             complement(5573..6961)
                     /locus_tag="PKH68_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HNX12520.1"
                     /translation="MTLQSRKFAADAAKIAFDTKHRNTIKFNISRYNAAFARGLARYR
                     DVEKAKTGAAAIKREVLKNWDHYLLEFEKNITANGTEVLWAENAQEATEYIRKILTEN
                     EARLLVKSKSMTTEEIELNETAESLGVESVETDLGEFIVQVAGEKPYHIVTPAMHKSK
                     EDIATLFHEKFSTPANSTPEALTDYVRGVLRKKYTAAEIGVTGANFLIADIGGISVTE
                     NEGNGIMSTAFPKVHIVIAGIEKIIPKMSQLGLFYPLLAAHGTGQQITAYNSIFTSPR
                     RADETDGPEKMIVILLDNGRTTIFADDEASESLACIRCGACLNGCPVYKTIGGYTYES
                     TYSGPIGSVITPFLKGFKDFSHLSFASSLCGRCNEVCPVKIPLTDILLANRRKTVEQG
                     LRPRAEKGIMSGFRLISSRRAGFDFFPSVMKNIGTAPFNYFGWGPHRKMPRFAKKSFS
                     ESYRINQNKQHT"
     gene            complement(6994..7890)
                     /locus_tag="PKH68_05985"
     CDS             complement(6994..7890)
                     /locus_tag="PKH68_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12521.1"
                     /translation="MEKKKITALLIGLGIVIAALIALLLYTMNKSSLKDEQIAATEEI
                     MTTEKQQLENEYKDLAGEYDGYTMTIHNDSLLKLFNEEKLKVNKLLEELRTTKTSNAV
                     RISELKKELATLRKVMVHYVNQIDSLNLQNKRLTNENYEVRQKYASASATAEQLSKEK
                     ESLNEVVTRAAILEVYNFSMTPLDKRNKATDRASKITTLQFNYTVGKNITAKPGMKTI
                     FMRLTRPDDEVMTKSPDTFFSYENKEIAYSLSKDFEYSGEARNDVLYWKVEEIMQIGT
                     YHADFFADGNRIGSFTFRIEKK"
     gene            complement(7915..>9144)
                     /gene="murF"
                     /locus_tag="PKH68_05990"
     CDS             complement(7915..>9144)
                     /gene="murF"
                     /locus_tag="PKH68_05990"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNX12522.1"
                     /translation="LFFALKGDNFNANAYALSALEKGCAYAVIDEEQYAVDERFILVD
                     NVLETLQNLARHHRQQLGTTIIGITGTNGKTTTKELIAAILKQKFNTLFTQGNLNNHI
                     GVPLTLLQLTSEHEIAVVEMGANHPGEIKQLAEIALPDYGIITNVGKAHLEGFGSFEG
                     VMKTKGELYQFINNNNGKIFLNTGNAFLITMSKKAGFLKPDRILSYELGNELKGHVAI
                     GKVLSCNPLLHLKCRNAAGEFEIATQLIGIYNAENVLAAATIGTKLGVSNELIKKGLE
                     EYIPQNNRSQLTVTEHNKIIADAYNANPTSMNAAIHNFTQMDVPAKTVILGDMLELGK
                     QSGEEHQKIVDLLKDKGFSSVYLVGKSFQATKNSFNCYATVNDLIQEIQKNPLKNNFI
                     LVKGSHGIHLEKILEYI"
BASE COUNT         2228 a         2001 c         1973 g         2842 t
ORIGIN      
        1 ccaaaatcct gagcaatggc gatagcgctg tttactacaa gccgtccgcc gattatcagt
       61 aaactcagcc cgacaacgat aaatccccag attttaaaac cagacattgg tttgatatct
      121 gtcgtaagct cgggaacttc gtcagtgttg ggttgccgga aggtgaaaaa gagaaacacc
      181 aggaacagcc cgaatagtaa tgcggcatcc cactttgaaa gtacattttc tttttgtccg
      241 agaattgcat ttgaaagaag cataaggatg aaagctacaa caaatgatat gggaatttct
      301 tttcgtacca tactgctttt caccgatatg gggaagatga gtgccactac gcccagtatt
      361 acaaaaagat tgaaattatt gctgccaata atattgccga aaacaatatc ggaatgtttg
      421 tcaaaagacg ctattacatt gacaaccatt tccggtgccg aagttccaaa tgcgacaatg
      481 gtaagtccga tgacaagatc tgaaatattg tgcttttttg ccagagccga agctccattt
      541 accagccagt cagccccttt aatcaacaat ataaatccca gcgttattaa aagaatatca
      601 agtatcatat tatttttttt taatccaaca tctgttttcc gctgtttctt tcccagaaaa
      661 caccgttggt ataaccctga atagcgggtg attgttcagc tttaagtagt cttttttctg
      721 cccagagcgc gtactcggtg ttcatttcaa taccaatgta atttcttcca agctttttag
      781 ctactaccga agtggtacct gaacccagaa aaggatcaaa tacaattcca tcgggcgggc
      841 aactggccag aatgattttg gcaatcagct tttcgggttt ctgggtaggg tgatcggtgt
      901 tttcgggcat cgaccagtac ggaacgctga tatcgtccca aaaattggat ggacccgtca
      961 gccggaactt accatcaccg gtctgctccc agtcttttgg ccggccgttt tcacggtacg
     1021 gagccagcac gcgtcttttt attttcacgg catctacatt gaaataataa tctttcggat
     1081 ttttcacccc gtaccagata tcttccatcc cattcttcca gttgtgagta gctgcgcggc
     1141 ctttttcacg ctgccaggtt atccggttga ggatagtgag attttcgctc attacccgct
     1201 ggagagcagc cgtgcatttc cagtcgccgc agaggtagag tgaaccattc ggtttcagta
     1261 ccgatactat tttagaaaac catgagcgaa gatatgccag gtactcgtca tcagtagaag
     1321 aagtaaattt aaaaccatga aaatttttgg taaggttgta aggtggatct atgatgataa
     1381 ggtcggccga gtttgccggc aaatagtcta ttacatcaaa taaatcggct aatattgttt
     1441 tattccggat ttcgtcgggt gtcaacggtt tgtttttctt tgggatcaac actcttttga
     1501 tcaactcttc ccgatcgttg tcggtcagtg tgagcgttct gtttctttta gctctttctt
     1561 tttccatttt tttcatctaa ttaattgttg ccgaaaaaaa cggcttgttt ttgagtttgc
     1621 aaatttccga aatatgcctg agaatgcaat ttgttgaata gttccctctt cccgtttggc
     1681 atttatagtt ataggggtaa aaaaataatg tatacccggc ttaaataatt gattatttat
     1741 aggataatta gcaactcaac tgttatattt gtagaataat ctatgttgaa atatgttata
     1801 tggatcagca tcaaaaattt atgcgcaaag ccattgggct ttcggttcaa aatctgaaga
     1861 aaggtggcgg accattcgga gctgtcattg tgaaagacaa taaagtgata gctaccggcg
     1921 tcaatcgggt gacaagccac aacgacccta cggctcatgc cgaaattgtg gctattcgca
     1981 gtgcaacgcg caagctccac agtttcaacc ttcagggatg cactatttat acttcctgcg
     2041 agccttgccc gatgtgcctt gctgctattt actgggcgca tctggataaa atatattatg
     2101 ccaacaccaa gacggatgct aaaaatatcg gctttgacga ttcctttatt tatgatgaaa
     2161 tagcacttga cccggacctc agaaagattg aaaccacccg attgctctcc gctgaagcca
     2221 tcacggcttt tgagcgttgg actgaaatgg acgaaaaaat ggagtattga tactgaatta
     2281 ttcataaaaa gcaattgaca ttgaaagatt atattccatt gtcaactgct tttttttatc
     2341 ctttccctgt tttatcctgc aatttcacca attagccaca tttcttcatc ggtgaaaacc
     2401 gtattttcaa gtgccccgat attatctttc aattgtttca cggaactggt ccctacgata
     2461 accgacgtta cctgacgatc gtgcagacac cacgccagcg ccatctgagc cagactttgc
     2521 cccctcgatt ttgcaaattc attgagtttt ttcagttttt tcaccagctc cggggtaacc
     2581 tgatcttttt gcagaaatcc gtacgattgt gctgcgcgtg agttttcggg tatgccgtta
     2641 aggtacttat tgctgagtaa tccctgtgcc agcggtgaaa aagcgataaa tccaatccct
     2701 ttttccttgg tcagcgggag aactgtttgt tcaatttcgc gggtgaaaat attgtatcta
     2761 tcctgattga taaggcacgg agtaccgttt tcggccaaaa tgcggtacgc ctgacgggct
     2821 ttgtcggttg ggtattttga aatccccacg tataaagctt ttccctgctt taccatatcc
     2881 gagagtgctc ccatggtttc ttccagcggg gtgttggggt catagcggtg cgaataaaat
     2941 atatccacgt attccagtcc cattcgtttc agactttgat taaggctggc tatgaggtat
     3001 ttgcgtgatc cccagtcgcc ataaggtccg tcccacatgg tgtgaccggc tttggtgctg
     3061 atgatcagtt cgtcacgata cgatgcaaaa tcttccttca gaatttttcc gaaattggcc
     3121 tcagcactac caaaaggtgt accgtagttg tttgccagat caaaatgagt gatgccgtta
     3181 tcaaaagcag tttcgagcat ttttcgggct tcggtcaggt cattgacgtc accgaaattg
     3241 tgccatagtc ccagtgaaag ttttggtagt tgcagtccgc tttttccaca gtagttatat
     3301 cccacagtgt gtgtatatct gtcggatgag ggtgtgtatg ccatagttgt aatatcaaac
     3361 aacctgtcag tgggttgaca ggttggagtt aatgtgtcgt tttctgcaat cagaatttta
     3421 ccgaaggggc tttttcggca cctttttcgg ctgcaaaata aggaatgtta ttgaaattga
     3481 aaataccggc aataatattt tttgggaaac gacggatggc tacatcatac tctttagcca
     3541 tttcgttgaa tttattgcgt tccactgaaa tccggttttc ggttccttcc agctgagcct
     3601 gaagttccag aaagttttga ttagctttca ggtccgggta attttcggcc acagctatca
     3661 atcgacccag cgcgctgctc aactgtccct gtgctgcctg aaattcctgc agtttttcgg
     3721 gtgtcaggtt ggtaggatct acggtaatct gtgtggcttt tgcgcgggcg gcaatcactt
     3781 cttcgaatgt cgatttttcg tgagttgcat aacctttcac tgtttcaacc agattaggaa
     3841 taaggtcagc gcggcgttga tactgatttt caacatttcc ccactgcgat tttacatttt
     3901 cctgtgagtt taccaatccg ttgtaacctc ccattcccca taaaaaaagg atagcgatga
     3961 gggcaataat aacaatgata cctgtctttt tcataaagtt ttcaattaat tattagtata
     4021 taagtatttt caaataagtt tttttgatgt ttaccaaccg ctggtagcac cgcctccacc
     4081 cgacatacct ccaccgaaac ttcctccgcc tccaccgccg aatcctccgc ctccgctgaa
     4141 cattcctccg cccacagcac ctccgaaaaa tccgctgttg tttcttttca gccgggggat
     4201 attgcggttg ttattttcct ggtaaccaca gaatttgcat ttatacgtca gtcgctctgt
     4261 gcctgcatga acataagtgg gagctacaac cattcgtttc gaatccagaa tgaatgcctt
     4321 cgtactgcat ttgggacagg tttcgtactg gctcttttta tccagcgtga agatggcctc
     4381 ttccttgcag ttttcacata caaacacatc gtaatccacc gctttcagtt cttcttccag
     4441 tttttgtgaa aggtgcaggt gtgcatcatc ttctttttcc gaaagaatat gcatttgact
     4501 tccacaggct ttgcacggaa tgggagcacg gcgcacgcgc gtcatcatgc tgcggccgta
     4561 taggtaggag ggcaacacgg tgagtggaac gggtaataaa aaaatcaggt acagtgaact
     4621 ggcaaagaaa agtataagtc ccagcagaat gaccggaaga taaagtccca ccatcgaagc
     4681 taccgaattg ttgttttcgc ggatcacttt gtagcgcgaa agattggtag agagcgtgtt
     4741 gtttctgagt gtttgagacg tgatgctgcg agtccacagg aaagtgagca aagccagcaa
     4801 aatataaccc gccagtgcaa acgggagcac ttcattccag ttgaacgcgg gcgctggagt
     4861 ttcttcagat acgggctcct tgttgatagc cgaaattatg ccattgacac ccgccagcat
     4921 gccggcatcg tagttgccat ttctgaattc gggcaccatt acagtcgatt gtattctttt
     4981 ggcaatagca tcggggatga cgccttccaa cccgtagccg gtttcaaatc gaactttttt
     5041 ctggtccagt acaaagagaa caagcagtcc gttgtcctct ttttctttac caatgcgcca
     5101 tttgtcgaac aggctccagg caaaattatc aatcacatca aaaccgatgg agttgagaac
     5161 aaccacagca acctgagcgc cattgttcag tgacagacta tctaatttcg catttaatgt
     5221 gttaacggtg ctttcgctca atattccgtc gggattgcta acgtagtgat gtgcattcgc
     5281 cgttatggga tttggaacac tttccacggt atattcctga gcgtaaaggg tacccagcca
     5341 cagaccgaga aggacgaaaa gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnactttcca gtcaatgtat ttgtcctctt tttcgcagga tgtaaatagc atggaaataa
     5521 gtgaaatcac cactataaaa agtatgtttt tagtgaattt ggttgtcatt ttttatgtat
     5581 gttgtttatt ttgatttatt ctgtaacttt ctgaaaatga tttttttgca aatcggggca
     5641 tttttctgtg tggaccccat ccgaagtaat tgaatggtgc tgtaccgata tttttcatca
     5701 ctgatggaaa aaaatcaaaa cccgccctcc gggaactgat taaccgaaat ccgctcatta
     5761 ttcccttttc tgcccgcggt ctcaatccct gctccaccgt ttttcgcctg tttgccagca
     5821 gtatatctgt aagcggaatt tttaccggac aaacttcatt gcatctccca caaagtgaag
     5881 aagcaaaact aagatgcgaa aagtccttaa atcctttcag aaaaggcgtt atcactgagc
     5941 cgatgggtcc gctgtatgtg ctctcgtacg tatatccgcc gatggtttta tataccggac
     6001 aaccattcag acaggccccg catcggatgc aggcaagtga ttcactggct tcgtcgtctg
     6061 caaatatagt cgttctcccg ttatccagca aaattacaat cattttttca ggtccgtcgg
     6121 tttcgtcagc acggcggggc gatgtaaaaa tgctgttgta ggcagtaatc tgctgaccgg
     6181 tgccgtgagc tgcaagaagc ggataaaaaa gtccgagctg gctcattttc gggattattt
     6241 tctcaatacc ggctatcaca atatgcactt tgggaaatgc cgtggacatg ataccgtttc
     6301 cttcattttc ggtcaccgat attcctccga tatcggctat gagaaaattg gctcctgtca
     6361 caccaatctc ggctgcggta tatttttttc gtaaaacacc tcttacgtag tcggtcagtg
     6421 cttcgggagt actgttggcc ggagtgctga atttctcgtg aaacagtgtg gctatgtcct
     6481 ctttggattt gtgcatagcc ggcgttacaa tgtgatatgg tttttcgccg gccacctgaa
     6541 caatgaattc acccaaatct gtttctaccg attccactcc cagactttct gctgtctcgt
     6601 tcaactcaat ttcttcggtg gtcatcgatt tgcttttcac caggagccgt gcctcatttt
     6661 cagtcagtat tttccggata tattccgtag cttcctgtgc attttcagcc caaagtacct
     6721 ctgtaccgtt ggctgtgatg tttttttcaa attccagcag gtaatggtcc cagtttttca
     6781 gcacctcgcg tttgatggca gcagcaccgg ttttggcctt ttccacatcc cggtatcgcg
     6841 ccagtccgcg ggcaaaggcg gcattgtagc gcgaaatgtt gaatttgata gtattgcggt
     6901 gcttggtgtc aaaagcgatt ttggcagcat cagcagcaaa ttttcgggac tgcagagtca
     6961 tcggaatttg ttaatatttc gattgtaaag atactatttt ttttcaatcc tgaatgtaaa
     7021 actccctatt ctgttaccgt cggcaaagaa atcggcgtga taggttccaa tttgcatgat
     7081 ttcttccact ttccagtaca aaacgtcatt gcgtgcttcg ccggagtatt caaaatcttt
     7141 gctgagcgaa taggcaattt ccttattttc ataactgaaa aatgtatcgg gacttttggt
     7201 cattacctca tcgtcggggc gtgtaagtcg catgaaaata gttttcatcc ccggtttggc
     7261 ggtgatgttt ttgcctaccg tatagttgaa ttgcagggta gtaattttgg aggcacggtc
     7321 ggtggctttg ttccgtttgt cgagcggtgt catgctgaag ttgtacactt cgaggattgc
     7381 ggctctggtt accacttcat tcaggctttc tttttctttg gatagttgct ctgcagtggc
     7441 cgatgcagag gcatatttct gacgaacttc atagttttca ttcgtcaatc gtttgttttg
     7501 taaatttagc gagtctatct ggttgacata atgcaccatc actttgcgca gggtggcaag
     7561 ttctttcttc aactcgctga tacgtactgc attcgaagtt ttggtggttc tcagttcttc
     7621 cagcagttta ttcaccttca gtttttcttc attgaaaagt ttgagcagcg agtcattgtg
     7681 tatggtcatg gtgtatccgt catattcgcc ggccaaatcc ttatattcgt tttccagctg
     7741 ctgtttctca gtagtcataa tctcctcggt ggctgcaatt tgttcatctt ttagcgagga
     7801 tttattcatg gtataaagta acagagcaat caatgccgct atcactatgc ccaggccaat
     7861 caacaaagcc gtaatttttt tcttttccat atttttaatg caatttatgt aggattaaat
     7921 gtattccaga attttctcca ggtgaattcc atgtgaacct ttcacgagga taaagttgtt
     7981 ttttaatgga tttttctgta tttcctgtat caggtcgttg actgtggcgt agcagttgaa
     8041 gctgtttttt gtagcctgaa aacttttacc caccagataa acggatgaaa agcctttatc
     8101 tttcaacaga tccacaattt tctgatgttc ctcgcccgat tgtttgccca gttcgagcat
     8161 atcacccaga attaccgttt tggcgggtac atccatttgt gtgaagttat ggatggcagc
     8221 gttcatgctg gttgggttgg cattgtaagc atcagctatt attttgttat gttcagtgac
     8281 agtcaattgc gagcgattgt tctgtggtat gtattcttcc agtccttttt ttatcaactc
     8341 gttgcttaca cccagctttg ttccgattgt tgcagccgcc agcacatttt cggcattata
     8401 gattcctatc aactgtgtgg caatttcaaa ttcacccgct gcatttctgc atttgagatg
     8461 gagcagcgga ttacacgaca gaaccttacc gatagccaca tgtcctttca gctcgttgcc
     8521 cagttcgtac gacagaattc ggtccggttt caggaatccg gctttttttg acatcgttat
     8581 cagaaaggca ttgccggtat tcagaaaaat tttgccgtta ttattgttga taaactggta
     8641 aagttcgcct ttggttttca tcactccttc gaatgaaccg aaaccttcga gatgcgcttt
     8701 acccacgttg gtaatgatgc cgtaatcggg cagtgcaatt tcagccagtt gttttatttc
     8761 acccgggtga ttggcaccca tttctaccac ggcaatttcg tgttctgaag taagttgcag
     8821 cagggtaagc ggcactccga tatgattatt gaggttgccc tgcgtgaata atgtgttgaa
     8881 tttctgtttg agaatggctg ctatcagttc ttttgtggtg gttttgccgt tggtgccggt
     8941 aatgccaatg atggttgtgc ccaattgctg ccggtggtgg cgggccaggt tttgaagtgt
     9001 ttccagcaca ttatcaacga gtataaaccg ttcatccaca gcatattgtt cttcgtcaat
     9061 gacagcatag gcacatcctt tttcaagtgc cgaaagtgca tacgcattgg cattgaagtt
     9121 gtcgcccttt agcgcaaaaa acag
//
LOCUS       DAOPZK010000077         9136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_8963_length_9136_cov_23.965422, whole genome shotgun sequence.
ACCESSION   DAOPZK010000077 DAOPZK010000000
VERSION     DAOPZK010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8963_length_9136_cov_23.965422"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2313
                     /locus_tag="PKH68_05995"
     CDS             <1..2313
                     /locus_tag="PKH68_05995"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX12523.1"
                     /translation="LSAFHDENVNGKLDASENGIPVEGFAFSNNAQPKMGPPNPQEML
                     FVIKENENATQNIRMIYFGIKPKELKTVEVVGEKKQFVNADADKTTYQVKDNAALSTG
                     SMRDAVRKLPGVVIGSSGDLNLNGKTVSIYVDGIPSNLSGSDLKNYMDGLPANTIEKI
                     ELIENPGASYEAKANGGIINIITRNTAPDNFGGTLNLHYGNSQNHKWSPSLMLNGRNK
                     KINWQLQSGYNWHQQDQFTDIKQLYTTFTPNVIFTNKSTQKNLNRNFYIRPMMNYRLS
                     ENSYIILNYNFNSYNNEHASNSIRESYNLTPAIKYSSVFNNPEATRNNELVLKYKTML
                     DTLGRSLQVTGYYSNYHKVAGAQSTQNFSTPLYGISANNLNLNNAYGKVDLELPFKII
                     RLTAGAKYNITNAHNLGKYNFNVLSSTIFDNPAYNQQIDFSYHEQNIAGYLEARKKLG
                     KLSATVGIRLEDLLYESEVAGADSVVGGRITQFFPTLNMLYTVIPNINLSGKYSRKIT
                     MPAYDQLDPNVNGYFDSYSKSAGNQNLKPNFYDNYSLSVTAFNYATLGSYLSYSKNIS
                     LLSTTVKPQSFISEKTSNDFSNIKMYGLYMSLPVPFGLITKGAEFFKKPANMSDMSYV
                     HFYVSCNFNQIKGLENPNTTKPLWMYNINTHIGLPYQFSLDANWFHMFKGNFQIYSFG
                     QPMDYWQVDLSRKFLKEKLQLTAEVTQMTAQFVKFSVPNADTDFISRTDGVTFWLKAS
                     YRFGGFKSKEETQIEVEKKALEGGQINVRK"
     gene            complement(2613..4058)
                     /gene="pyk"
                     /locus_tag="PKH68_06000"
     CDS             complement(2613..4058)
                     /gene="pyk"
                     /locus_tag="PKH68_06000"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNX12524.1"
                     /translation="MTQKFTKIVATISDKRCEVDFIRQLYQEGMNVVRMNSAHLQREG
                     FLRIINNVREVSHRIAILIDTKGPEVRTTIAENDSILLKTGDTVKVTGDVDLVSTKDC
                     IAVNYPHFVRDLHVGDDILIDDGEIDLKVVTKDDNFLICTVLNDGKLGSRKSVNVPGV
                     RINLPSLTPKDKENILFAIEQNVDFIAHSFVRSKQDLLDIQEILDAHNSPIKIISKIE
                     NQEGVDNIDEIIQHCYGVMVARGDLGIEVPQERIPGIQRKLIRKCVKAHKPVIVATQM
                     LHSMIENPRPTRAEVNDVANAIYYRTDALMLSGETAYGKYPIEAVRTMATIALEAENT
                     KMPENDIPIEFGKGNVTAYLAHAAAEAGKNIDIKAIITDTFSGRTARYLASYRSSDPI
                     LAFCYNERASRELALSYGVYSYYKEELVEDERNYFTTLLHNIIGRGIISPEDIICYLS
                     GPTSGSFGTTFLEINTANTLLSEIDNYTLKK"
     gene            complement(4070..4486)
                     /gene="aroQ"
                     /locus_tag="PKH68_06005"
     CDS             complement(4070..4486)
                     /gene="aroQ"
                     /locus_tag="PKH68_06005"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNX12525.1"
                     /translation="MKKIIIINGPNLNLLGKREPEVYGNTDFETFFSTLKSEFPDMEL
                     SYYQSNSEGEIIDKLQETGFTFDGIVLNAGAYTHTSLAIADSIRSINAPVVEVHISNV
                     FARESYRHHSYLSAACKGCIVGFGLKSYSLAVSSFQ"
     gene            4702..5166
                     /locus_tag="PKH68_06010"
     CDS             4702..5166
                     /locus_tag="PKH68_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12526.1"
                     /translation="METANSILNKYKPGVTKRVLLIVAGLVWTFAGGMLLIKGFQMLS
                     VQPHNLWIKLIVSFMGGVLFFVLLFNKLSLKHIKRILFSEVEKPCAFSFFNWKGYLMM
                     ILMITTGITLRTTGVVSPDILSVLYIMMGIPLLSSALRFYINGFINHSTNKK"
     gene            5174..5761
                     /locus_tag="PKH68_06015"
     CDS             5174..5761
                     /locus_tag="PKH68_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12527.1"
                     /translation="MQFPLNFTFKITTISNDFRVNDATGAEVAFVRQKLFKLKEEVQV
                     FNDESRSQLNFTVKANKWLDFSAAYVFYDNQQRELGRIVRRGWKSLWKSSYDIYDENQ
                     KQDFSVNEENPLIKVFDGLFGDIPILGMFTGYFFNPTYLVTRPDGTAIARLSKEKSFF
                     GRRFSVDKLAEFESGEENRLLLGLMMMILLERQRG"
     gene            5803..6867
                     /gene="corA"
                     /locus_tag="PKH68_06020"
     CDS             5803..6867
                     /gene="corA"
                     /locus_tag="PKH68_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNX12528.1"
                     /translation="MSRITRKRKEDIGLSPYEMKFRGQKKVERTTMELFDFNLDFVHE
                     LPVKSPDKLTAYQTKDTISWLNVDGLDDVTLMKKLAEIFRIDNNIMSEIMNPAGRPKV
                     QEFEEGLLVTLKMLQINERTNKLGVENLSLVIKNDFLISFQEDKGDFFDTVRERIRKH
                     KNKIRTAGPDYLAFALLDMVVDNYIYIVGILGEKIENLEEKMTGNVNKQLPELINLYK
                     QELNFLRRYIKPAKEMILTLAKMESEFIHDENRMNYKELQDNINEANDLTDSYREMLY
                     DLLNIYHTSATTRLNDIMKVLTMISVIFIPLTFIVGVYGTNFDYLPELNWHYGYFIMW
                     GIMVVVALLMIWYFRRKKWY"
     assembly_gap    7188..7287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7288..7868
                     /locus_tag="PKH68_06025"
     CDS             <7288..7868
                     /locus_tag="PKH68_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNX12529.1"
                     /translation="NIPVLRQQHPSSDGFCYSLADFLSEENDYLGAFANTVTGTEVMA
                     QRFEAEDDLYKSILVKTLSDRLAEATAEWLHWKVRKEFWGYAPDENLSVEEMLKTQYK
                     GIRPAVGYPSLPDQSIIFELDKLLNYNKSGISLTENGAMYPNASVSGLYFAHPLSRYF
                     MIGKIDENQLADYARRKGKTTGEMRKWLAGNI"
     gene            8023..9132
                     /locus_tag="PKH68_06030"
     CDS             8023..9132
                     /locus_tag="PKH68_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNX12530.1"
                     /translation="MKYGFFVFILAFMLTMIVYTIVRGSQALAPYGALKTVYLVSMIT
                     LTATLFGGLFFESQLPHSVGKVLSFVGYSFFIVLAYLFISFLLVDIIRAVNHFLPFIT
                     DMKIFRLSAMVITLTVLGVLMVWGNYKFNHPKTVTMTVESSKPLQHKEIKIVAISDVH
                     LGVSIDKSKLKKYVAMINAENPDLVLIAGDLIDRSLQPVISQKMDEELRMIKAPMGVY
                     GIFGNHEYYGEGNHHIADFYKKSNITLLQDSCVLLKDEIYLAGRDDKHNPHRKNLEDI
                     LAGRTGKPILLLDHQPFHLEDAEKNNVDLQISGHTHNGQFFPANLIVKSMFEMGYGSH
                     KRGNTNYYISSGIGLWGPQYRLGSQSEMVVINFRY"
BASE COUNT         2721 a         1906 c         1918 g         2491 t
ORIGIN      
        1 ctatcggcat ttcacgatga aaatgtaaac ggaaaactgg atgcatctga gaacggcata
       61 cccgtagaag gatttgcatt ctccaacaat gcccaaccca aaatggggcc tcccaatcca
      121 caggaaatgc ttttcgtgat taaagaaaac gagaatgcga cccaaaacat ccggatgatt
      181 tatttcggca taaaaccaaa ggaactgaaa acagtagaag tggtgggaga gaaaaaacaa
      241 tttgtaaatg cggatgccga caaaacaacg tatcaggtaa aagacaatgc ggcactttcg
      301 acgggctcca tgcgagacgc cgtaaggaaa ctgccgggag tggtaatcgg ttcttcgggt
      361 gatctgaatt tgaacgggaa aacggtaagt atatatgtag atggcattcc gagtaacctg
      421 tcgggatcag atctgaaaaa ttacatggac ggtttgcccg ccaatacgat tgaaaaaatt
      481 gagctgattg aaaatccggg cgcatcgtac gaagctaaag caaatggcgg aattatcaac
      541 attattacac gaaatacggc acccgataac ttcggtggca cactcaacct gcattacggc
      601 aacagccaaa accacaaatg gtctccttcg ctgatgctga acggacgcaa taaaaaaata
      661 aactggcagc tgcaatcggg gtataactgg catcagcagg accagtttac ggacattaaa
      721 caactgtata ccacgtttac accaaacgta attttcacta ataaatctac acagaaaaac
      781 ctgaaccgaa atttttatat tcgtccgatg atgaattacc ggctgagtga gaattcgtac
      841 atcattttaa actacaattt caactcctac aacaacgaac acgcgagcaa ttctatccgc
      901 gaaagttaca accttactcc cgctataaaa tattcatccg tattcaacaa tcccgaagcc
      961 acccgcaaca acgaacttgt tctgaaatac aaaacaatgc ttgacacgct gggtcggtcg
     1021 ctgcaggtaa caggttatta ttcaaactac cacaaagtgg ccggggcgca atccacacaa
     1081 aatttttcca ctccgctgta tggtatcagt gccaacaacc tgaacctgaa caacgcctac
     1141 ggaaaagtag atctggaact tccgttcaaa ataataaggc tgactgccgg tgccaaatac
     1201 aacatcacga atgcgcacaa tttgggtaaa tacaacttca acgtgctttc gtccaccatt
     1261 tttgataatc cggcttacaa tcagcaaatc gacttcagct accacgaaca aaacatagcc
     1321 ggatatctgg aagcgcgcaa aaaactgggg aaactgtcgg caactgtcgg cataaggctt
     1381 gaagacctgc tttatgaaag cgaagttgca ggtgccgaca gcgttgtggg gggcagaatt
     1441 acccaatttt tccctaccct taatatgtta tacactgtaa ttccaaatat taatctttcg
     1501 gggaaatact cacgcaaaat aacgatgccc gcttacgacc aactcgaccc gaacgtaaac
     1561 ggctatttcg acagctacag caaatcggcc ggaaatcaaa acctgaagcc caacttttac
     1621 gataattatt cgctgagtgt caccgctttt aattatgcca ccctgggaag ttacctcagc
     1681 tactcgaaaa atattagttt gctttccacc accgtaaagc cacaatcttt catttccgaa
     1741 aaaacttcaa acgacttcag taatataaaa atgtacggtt tgtacatgtc gcttccggtt
     1801 cctttcggac tgattacaaa aggggcagaa tttttcaaaa aaccggccaa catgtccgac
     1861 atgagttatg tgcattttta tgtgtcctgt aattttaacc agataaaagg gctggaaaac
     1921 cccaacacca caaaaccgct ctggatgtac aatattaata cacacatcgg gttgccctac
     1981 caattctcgc tggatgccaa ctggtttcac atgtttaagg gcaattttca gatctactcg
     2041 ttcggccagc cgatggatta ttggcaggtg gatttgtcgc gaaaattcct gaaagaaaaa
     2101 ctgcaactga cagccgaagt gacacaaatg acagcccagt ttgtaaaatt cagcgtaccg
     2161 aatgccgaca ccgactttat aagccgaacg gatggtgtaa ccttttggct gaaagcatcg
     2221 tatcgtttcg gtggtttcaa aagcaaggag gagacgcaaa ttgaagttga gaaaaaagcg
     2281 ctggaaggcg gccagataaa cgtgaggaaa tagacactaa ctgaaaacaa cagagacacc
     2341 cggttttgga tgtctctgtt tggtttttaa cactgttgtc cgataagatt taatatattg
     2401 ccactctgtg gcaataagtt ctttttttct catcttacta caaagattta gccacgctgt
     2461 ggctgttttt gcaacagcgt tgcaaaatat ttgtagcgat ttggcaacaa cagaaaagca
     2521 agcccctgcg gggcttaatg ctattatttg tttcgattac aacaaattat aaacttttca
     2581 atgtttttcg tcgggcggca atggttttta tcttatttct ttaatgtata attatcgatt
     2641 tcggagagca aggtattcgc cgtatttatt tccagaaatg tagtaccgaa cgagccactg
     2701 gtaggtccgc tcaggtagca gataatatct tcgggggaaa taatgccgcg accgatgata
     2761 ttatgcagca gtgtggtgaa ataatttctt tcatcttcca ccagttcttc cttgtaataa
     2821 gaatacacgc cgtaggataa tgccagttcc ctggaagccc gttcgttgta gcaaaaggca
     2881 aggataggat cggagctgcg gtacgaagcc aggtatcggg cagttcggcc actgaaagta
     2941 tcggtaatga tggctttgat atcaatattt tttccggctt cggctgctgc atgtgccaga
     3001 taggctgtta cattgccttt accaaattca atcgggatat cgttttcggg cattttggtg
     3061 ttttcggctt ccagtgcaat tgttgccatt gtcctcacgg cttcaatcgg atattttccg
     3121 taagccgttt cgccgctgag catcaatgca tcggtacggt aataaattgc attggctaca
     3181 tcgttcactt ctgcacgggt ggggcgcgga ttttcaatca tcgaatgaag catttgagta
     3241 gccacaatca caggtttatg ggcctttaca cacttgcgga taagcttgcg ctgaatgcct
     3301 ggaatgcgtt cctgaggtac ttcgataccg aggtcgccgc gggcaaccat caccccgtag
     3361 caatgctgaa taatttcatc gatattatca acaccctcct ggttttctat ttttgaaata
     3421 atttttatcg ggctgttgtg cgcgtcgaga atttcctgaa tatcgagcaa atcctgtttg
     3481 gagcgaacaa acgaatgggc aataaaatcc acattttgtt cgatagcgaa gaggatgttt
     3541 tctttgtcct ttggtgtgag agaaggtaaa ttaatacgaa cacccggcac attaacactt
     3601 ttacgacttc cgagtttgcc gtcattgagt accgtgcaaa ttaaaaaatt atcgtctttt
     3661 gttacaacct tcagatcaat ttctccatcg tctatcagta tatcgtcgcc cacgtgcaga
     3721 tcgcgtacaa aatgaggata gttcacggca atacagtctt tggttgagac caggtcaaca
     3781 tcacctgtta cttttacggt atcgccggtt ttcaacagta tactgtcatt ttcggcaatg
     3841 gtagtacgga cttcaggtcc tttggtatcg atgagtatcg ctatccggtg gctcacttca
     3901 cgtacattgt ttataattct gaggaaacct tcgcgttgca ggtgtgcaga attcatcctc
     3961 accacgttca ttccttcctg ataaagctgt ctgataaaat ccacttcaca gcgtttgtcg
     4021 ctgatggtgg ctactatttt tgtaaatttt tgtgtcattg tggggctttt tactgaaaac
     4081 tacttacagc caggctgtat gactttaatc caaatcctac aatacagccc ttgcaggctg
     4141 ctgagaggta ggaatgatga cgatacgatt cgcgggcaaa aacgttggaa atatgcacct
     4201 ctaccaccgg tgcgttaatg gagcgaatgg agtcggcaat agccagcgag gtgtgcgtat
     4261 aggcacctgc gttaagtaca atgccgtcga aggtaaaacc ggtttcctgc aatttgtcga
     4321 taatttcacc ttcggaattt gactgataat aagacaattc catatctgga aattcacttt
     4381 tcagtgttga aaaaaatgtt tcgaaatccg tgtttccata aacttcaggt tcccgcttac
     4441 caagtagatt gaggttggga ccattaatga tgataatctt tttcatcggc gagttgcttt
     4501 gaattcgggg caaaattacg aaaataaatc agtaaccgaa aataacatta agtcccggtt
     4561 cgtgttttat tcagatgatt aaacaattga aacaaataag agtttatcat tttgattgat
     4621 tttgatttta ctttgggatt ttatcaaaat ataactgaac atacagcgat acaaaaataa
     4681 ttcagtattt ttggcaaaaa tatggaaaca gcaaattcta ttttgaacaa atacaaaccc
     4741 ggtgtaacga aacgtgtcct gttgatcgtt gccggactgg tttggacttt tgcaggagga
     4801 atgttgctta tcaaaggctt tcaaatgctg tcagttcaac cgcataattt gtggattaaa
     4861 ctgattgtca gttttatggg aggagtttta tttttcgtat tgcttttcaa caaattgtcg
     4921 ttaaaacaca taaaacgcat cctgttttcg gaggttgaaa aaccctgtgc attctcgttt
     4981 ttcaactgga aaggctactt aatgatgata ctgatgataa caaccggaat tactttacga
     5041 acaaccggtg ttgtttctcc cgatattttg tccgttttgt atatcatgat gggcattcca
     5101 ctgctttcgt cggccttgcg tttctatatc aacggtttta taaatcattc aactaacaaa
     5161 aaataatacg ctcatgcaat tcccgttaaa tttcacattt aagattacca ccattagcaa
     5221 tgatttcagg gtaaatgatg ccacaggcgc ggaagttgcg tttgtgcgcc aaaaattatt
     5281 caaactcaaa gaagaagtcc aggttttcaa cgacgaaagc cgatcacaac tcaatttcac
     5341 agtaaaagcc aataaatggc tcgatttttc ggctgcctat gtattttatg ataatcagca
     5401 acgggaactg ggacgtatag tgcgccgggg ctggaagtca ttgtggaaat cgtcttacga
     5461 catttatgat gaaaaccaga aacaggattt cagtgtgaac gaagaaaacc cgcttatcaa
     5521 ggtttttgac ggattgttcg gcgacatacc catactcggt atgtttacgg gctatttttt
     5581 caatccgacc tacctggtta cccgtcctga tggaacagcg atagcgcgct tatcaaaaga
     5641 aaaatcattt ttcgggcgcc ggtttagtgt tgacaaactg gctgaattcg aatcgggtga
     5701 agaaaatcgc ttgctactgg gtctgatgat gatgattctg cttgagagac agcgcggata
     5761 acacctgaaa aaattaaatc cttgaataat aaacaccctg tcatgtcgcg cataacgaga
     5821 aaacgaaaag aagatatcgg tctgtcgcct tacgaaatga aatttcgtgg ccagaaaaag
     5881 gttgaaagaa ccacgatgga gttgttcgat ttcaacctgg attttgtaca tgaactgccg
     5941 gtcaaatcgc cggataagtt gacggcttac cagaccaaag acaccatttc gtggctcaat
     6001 gtcgatgggc ttgacgacgt gaccctgatg aaaaagttgg cagaaatatt cagaattgac
     6061 aataatatta tgtcagaaat catgaatccg gctggccggc ccaaggttca ggaattcgaa
     6121 gagggactgc tcgtaacact caaaatgctg caaatcaacg aacgcaccaa caaactggga
     6181 gtggaaaatc tcagtcttgt aattaaaaat gactttctga tatcttttca ggaagacaaa
     6241 ggcgatttct tcgacacggt acgcgaacgg atacgcaaac ataaaaacaa gatacgcacc
     6301 gccggacccg attatctggc atttgcgctg ctcgatatgg ttgtggacaa ctacatttat
     6361 attgttggga ttttgggtga aaaaattgaa aatcttgaag aaaaaatgac cggaaatgtc
     6421 aataaacagt tgcctgagct gattaacctt tacaaacagg aactcaactt tctgcgccgt
     6481 tacataaaac cagccaaaga gatgatactc accctggcta aaatggagtc tgaatttatt
     6541 catgacgaaa acaggatgaa ctacaaggag ttgcaggaca atatcaacga agccaacgac
     6601 ctgacggaca gctaccgcga aatgctctac gacctgctca acatctacca tacctccgcc
     6661 acaacccgac tgaatgacat aatgaaagta ttgacgatga tctcggtaat tttcattccg
     6721 cttaccttta ttgtgggtgt atacggcacc aatttcgact atttaccaga gcttaactgg
     6781 cattacggtt actttatcat gtggggaatt atggtggtgg ttgctctact gatgatctgg
     6841 tatttccgaa gaaaaaaatg gtactgatga acttcagtat cgtttagttg tttaattcct
     6901 actcagcccc ctaaatcccc cgaaggggga cttttttata ctacagaaat tgagcacttt
     6961 acaaattgta atggcagcaa atcgagtaaa ataaaatgag ttggtaggaa gggggaaaaa
     7021 tattattctg ttattgaaaa agcatcaaag ttaaatatgt catacatccc atcagcaccc
     7081 aataaaaccg gagtttatta cctgacagac atcagtctgc gtgaaattgt tccatttatc
     7141 gactggcggt tttattttct tgcctggcga ctttcggggc actacgannn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnntca acatacccgt tctgcgtcag caacatccat
     7321 catccgatgg gttttgctat tccctggccg attttttatc tgaagaaaac gattacctgg
     7381 gcgcatttgc caatactgta accgggaccg aagtgatggc acaacgattt gaggccgaag
     7441 atgacttgta taagtccatt ctggtgaaaa cactctccga ccgcctggca gaagctaccg
     7501 ccgaatggct tcactggaag gtgcgtaaag aattctgggg ctatgctccc gatgaaaatt
     7561 tatcggtaga agaaatgctc aaaacccagt acaaaggcat cagacccgcg gtgggctacc
     7621 cttcacttcc cgatcaatcc atcatcttcg agctggacaa actgctgaat tacaacaaat
     7681 caggcatttc gctcaccgaa aatggagcga tgtatcccaa tgcctctgtc agtggattgt
     7741 attttgctca tccgctgtcc aggtatttta tgattggaaa aattgatgaa aaccaactgg
     7801 ctgactatgc ccgcagaaag ggaaaaacta ccggcgaaat gcgaaaatgg ctggcaggga
     7861 atatataatg ccccaaaggc cccctaaatc ctccgaagga aaggccccct aaatccccct
     7921 caagggggac ttaaagaaaa aaacagttgc tcctcatact tgtgatttga ataatggctg
     7981 atgtttgaaa tctaataatc taaaaagtaa tatgttataa ttatgaaata cggttttttt
     8041 gtttttattt tagcttttat gcttacgatg attgtttaca ccattgtcag aggcagccag
     8101 gcgttggctc cttacggtgc actcaaaacc gtatatctcg tgtcgatgat tacgcttact
     8161 gcaactttgt ttggaggctt atttttcgaa agtcagctac cacacagtgt tggcaaggtg
     8221 ttaagttttg tgggttatag ctttttcatc gtgttggcat atctgtttat ctcctttttg
     8281 ctggtagata tcatccgggc agtgaaccat tttttgcctt ttattacgga tatgaagata
     8341 tttcgacttt ccgccatggt tattaccctc acagtactgg gcgtattgat ggtttgggga
     8401 aattataaat ttaatcaccc caaaacagta accatgacag tggaaagcag caaaccctta
     8461 caacacaaag aaattaaaat agttgccatc agcgatgtcc atcttggagt ttctattgat
     8521 aaatcaaaac ttaagaagta tgtagcaatg atcaatgccg aaaacccgga tttggtactc
     8581 attgccggtg acctgataga ccgctccctg cagcctgtaa tcagtcagaa aatggatgaa
     8641 gagctacgta tgataaaagc cccgatgggt gtgtacggaa tttttggcaa tcatgaatat
     8701 tacggtgaag gcaatcatca tattgctgac ttttacaaaa aatcgaacat tacattgcta
     8761 caggatagct gtgtgctgct gaaagacgaa atttatctgg ccggaaggga cgataaacac
     8821 aatccacacc gcaaaaatct ggaggatata cttgccggac gtaccggtaa acctatcctg
     8881 ctgctcgatc atcagccttt tcacctggaa gatgcggaaa aaaataacgt ggatttgcag
     8941 atttcagggc atacccacaa cgggcagttt ttccctgcca atctgatagt taaaagtatg
     9001 tttgagatgg gatatggatc tcataaaaga ggaaatacca attactatat atcatccgga
     9061 attggtctct ggggaccaca gtaccgcctg ggaagccaga gtgaaatggt ggtaattaat
     9121 ttcagatatt aaaaaa
//
LOCUS       DAOPZK010000078         9091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_9032_length_9091_cov_25.105024, whole genome shotgun sequence.
ACCESSION   DAOPZK010000078 DAOPZK010000000
VERSION     DAOPZK010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9032_length_9091_cov_25.105024"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..681)
                     /locus_tag="PKH68_06035"
     CDS             complement(247..681)
                     /locus_tag="PKH68_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNX12531.1"
                     /translation="MVQLQNDELFDILVGKISATINRTFLRTFTAEGIDISIEQWTVL
                     ACLWNKDKVTQQTLCSLTAKDKPSMTRLIDKLERSNLVTRVSDHNDRRINLIHLTDKG
                     LSLQKKATDLVQKIVGKALQNITEEELNVSRSVLKKIITNLN"
     gene            complement(963..1148)
                     /locus_tag="PKH68_06040"
     CDS             complement(963..1148)
                     /locus_tag="PKH68_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12532.1"
                     /translation="MEFEEKEAKIQGRVFASSHLYHFFSPLFYRCLKKCILRNKQLHC
                     LLEFFADKITTQQEENF"
     gene            complement(1280..1702)
                     /locus_tag="PKH68_06045"
     CDS             complement(1280..1702)
                     /locus_tag="PKH68_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12533.1"
                     /translation="MKKSIIFLCTLILLVGCIGTPQKRAEMNVKKFIKETANGNSATI
                     KSVSFGPLTTIYLKEDSAYLQARDSLFYYKRQLTETTNQFRLADLQTKAKEFAAEVKN
                     REEFYKERKYKMVHRVKNENSDEFESTFYLNGNFEVVR"
     assembly_gap    1914..2013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2014..4137)
                     /gene="rpsA"
                     /locus_tag="PKH68_06050"
     CDS             complement(<2014..4137)
                     /gene="rpsA"
                     /locus_tag="PKH68_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNX12534.1"
                     /translation="MQENEKFAEEVEQTPEAVEETQQAVAEETAPVAEEVIEEAPVAE
                     ETAPVAEETAPAAEEVIEEAPAAKKETPKYVKPTGPVTDFDWDSYEKGDVMTGKSKDE
                     LEKIYDDTLNAIKDKEVVEGTVTSINKREVVVNVGFKSDGVVSMSEFRYNPELKVGDK
                     VEVYIESQEDKKGQLILSHKQARATRSWDRVNAALESQEVVKGFVKCRTKGGMIVDVF
                     GIEAFLPGSQIDVKPIRDYDIFVNKTMEFKVIKINQEFKNVVVSHKALIEEEIEQQKK
                     EIIGKLQKGQVLEGVVKNITSYGVFIDLGGVDGLIHITDLSWGRVSHPEEVVSLDQKL
                     NVVILDFDDEKKRIALGLKQLSAHPWDALDPNLKVGDKVKGRVVVMADYGAFVEIAPG
                     VEGLIHVSEMSWSQHLRSAQDFLKVGDEIESVILTLDRDERKMSLGIKQLKSDPWENI
                     ETRYPLASKHTAKVRNFTNFGVFVEIEEGVDGLIHISDLSWTKKIKHPSEFTQIGANI
                     DVQVLEIDKDNRRLSLGHKQLEENPWDALESLFAVDSLHEGTIVEMMDKGAVVSLPYG
                     VEGFVTPKHLVKEDGSQAQVDEKLQFKVIEFNKDAKRIILSHSRIHEEAKRAEKAADG
                     EETKKATKRPTKKDDASAVVATPVIEKTTLGDIQSLADLKDQMVSEAAEKLVAKEAKK
                     AKVAKTEKTVAEEAEVAEVKEEKAPK"
     gene            complement(4402..5865)
                     /locus_tag="PKH68_06055"
     CDS             complement(4402..5865)
                     /locus_tag="PKH68_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNX12535.1"
                     /translation="MIKNLFIRKSLAQIEIQAEESKDSLRKHLNLTNIILLGVGCIIG
                     AGIFVLTGTAAALHAGPAIAISFIVSAFGCLLAGLCYAEFASMIPVSGSAYTYGYATM
                     GEFIAWIIGWDLILEYLFGSATVAVGWSGYVSSFLGDLGLHLPASICQSPFIMDASGW
                     HATGAIINFPAVFIVALMTTLLVIGIKESASFNNIIVLIKVTVILLFIGFGIAHINLD
                     NWTPFIPANTGKFSEFGWSGILTGGAVVFFAYIGFDAVSTTAQEAINPKRDVPIGILG
                     SLIVCTILYVAVSLVMTGIVNYTELNVPAPIAVAIDAAGKSLQWLSPIIKIGAIAGLS
                     SVVMVLLLAQSRIFYSMANDGLLWKSFGKTHPKFKTPYITSIVTGCFAALFAGLFPIG
                     LLGELVSIGTLLAFVIVSIGLIVLRKKEPHVHRAFRTPWVPVVPILGALVCLAQMAAL
                     PLDTWIRLLGWMAIGFIIYFTYGIKHSKVRQEFSNQK"
     gene            complement(6051..7808)
                     /locus_tag="PKH68_06060"
     CDS             complement(6051..7808)
                     /locus_tag="PKH68_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNX12536.1"
                     /translation="MKEIKFSLVYRDMWQSSGKYVPRIDQLQKIAPVIIDMGCFARVE
                     TNGGASEQVNLLYGENPNPSVRAFTKPFNEVGIQTHMLDRGLNGIRMNPVPADVRALM
                     YKVKKAQGVDITRIFCGLNDVRNIALSIKYAKAAGMIAQAAMCITYSEVHTVEYYVNM
                     AEQLIAEGADEICLKDMAGVGRPDMLGKIVKAIKTSHPEIPIQYHGHSGPGFSVASML
                     EVAKAGVDYLDVAMEPLSWGMVHPDVITIQAMLKDAGFKVPEINMKAYMEARRLTQSF
                     VDDFLGYFIDERNKYMTSLLIGCGLPGGMMGSLMADLKGVHAGINTYLKNNNKPELST
                     DEMLVMLFDEVGYIWPKLGNPPLVTPYSQYVKNIALMNVMAMIKGQPRWGMIDKNSWD
                     MILGKAGKLPGKLAPEIIELAKKNNYEFFEGNPQDNYPDELPKFIKEMEELGWERGQD
                     DEELFEFAMHEKQYRDFKSGVAKERFDKELEAAIKEKYAQSEIQIPDIRALKYPNAEP
                     IVSTASGRLMWELDFNEVSVEPPHGRHFKKFDLICAIQTNYGIEPIGSFCDGRIVGVE
                     KKQGELVRKGEALAFIEKD"
     gene            8223..>9091
                     /locus_tag="PKH68_06065"
     CDS             8223..>9091
                     /locus_tag="PKH68_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNX12537.1"
                     /translation="MKFDTSDFLVKPGSKVKLKEMDTRFSAGLNKKKGEKELLKNISE
                     LAEFQEKFWADNRYSLLIILQAPDAAGKDGAIRHVMTGLNPQSCRVHCYKVPSKEELD
                     HDYLWRHYKDLPERGMIEIFNRSHYENVLATKVNPQYLMAENIPGLDKPEKADKKFWN
                     ERYEQINNFEKHIYQNGTHILKFFLNLSKEEQKERLLARLNEPEKNWKFSSADLTARA
                     QWDEYQKAYEEMMEKTSTEYAPWHVIPADKKFFARVAIGEIIVEKLKSLDLRYPEGES
                     AEDLQKAKAMLMS"
BASE COUNT         2524 a         1895 c         1778 g         2794 t
ORIGIN      
        1 gatacatctg tatggaagtg attaaagcga taataactaa tacctttttt ttcatctgtt
       61 tataatttat ttatcggccc cctaaccccc taaaggggga atattgagcg tctttcattt
      121 gttatgcgtt atttaatcat gccattgtgt gtccaattta ttttgttgga catgggtgtt
      181 tcatgttgct ttatattgtg attgaataaa caatagttgt ctgtgcaact atatgtccaa
      241 aaaaatctaa tttaaatttg taataatttt tttcaatacc gatcggctga cattcagctc
      301 ttcctccgta atgttttgga gcgctttgcc aacaattttc tgaaccagat cggttgcttt
      361 tttttgcagg gataaacctt tgtctgtaag atgaattaaa ttgattctgc ggtcattgtg
      421 atcggaaaca cgtgtcacca ggttgctcct ttcaagtttg tcgataagtc ttgtcatgct
      481 tggtttatct tttgctgtca agctgcaaag tgtttgctga gttactttat ctttattcca
      541 taagcaggcc agtactgtcc attgctcaat ggaaatgtca ataccttcag ccgtaaatgt
      601 gcgcagaaag gttcggttga tggtggcgga aatttttcca acaaggatgt caaataattc
      661 atcgttctgt aattgtacca tgatagttga ttgattaatg gttgtctaaa caacgatgca
      721 aaattactgc cattttcaat atgttgtact cagtttagca atatttaatg tattttcaca
      781 aaaagtacag gtaatgaaaa ctttattaac gatacaatct gcaagaatac agaaacggat
      841 tatcatcgtt ataatgcggg cttttgacgg tcatgctggg taaaccaagg agagggtatc
      901 tcggaaaaca aagatgtaaa atggaaattc tgagaatggc gggtatgtcg agcagagcag
      961 ttttaaaaat tctcctcctg ttgtgtagtt attttatcgg caaagaactc aagcagacaa
     1021 tgcagctgtt tatttcttaa gatacatttt ttcaggcagc gataaaaaag tgggctgaaa
     1081 aaatgatata gatgagaaga agcaaaaact cttccctgaa tttttgcttc tttttcttca
     1141 aattccatgc aatcgcagca attaatggaa ttattaaatc ccgtatttgt ggaaaaatac
     1201 tttttgatat atttatataa tagtcatgca tttaaatgaa aatcagggag gatctagata
     1261 tccatcaatg tgaatattct tatcgtacca cttcgaaatt gccgttgagg taaaaagtag
     1321 attcgaactc atcgctattt tcatttttta cccggtgaac catcttatat tttctctctt
     1381 tataaaattc ttcccggttt ttaacttctg cggcaaattc tttggctttt gtctgcaaat
     1441 cagctaaccg gaattgattg gtagtttcgg ttaattgtcg tttgtaataa aaaagcgaat
     1501 cccgggcctg aaggtaagca ctgtcttcct tgaggtaaat tgtagtaagc ggaccgaaag
     1561 aaactgactt gattgtagcg gaattaccgt tagctgtttc tttaataaac tttttcacat
     1621 tcatttcggc tctcttctga ggcgttccga tgcaacccac caaaagaatt aatgtgcaaa
     1681 gaaaaataat cgatttcttc atgttttttg ggatttattg attaatgaat tacaaacata
     1741 cactaaattt ttagatttac gattactact cagattctaa ctaactattg gttcaatatt
     1801 tattcctttt tcattttcaa ttttttcatt ttccatacac tcggcattaa tcctttcaaa
     1861 atatattttt taaaagcaag aaaggctgat cattaatctg aacagccttt ctcnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttttggt gccttttctt cttttacttc
     2041 agcaacttct gcttcttctg caacagtttt ttctgttttt gcaacttttg cttttttagc
     2101 ttccttagct accagttttt cggcagcttc cgataccatc tgatctttga gatcagccag
     2161 tgactgaatg tcacccaatg tggttttttc gattaccggg gtagctacca ctgcagaagc
     2221 atcgtccttt ttagttggac gtttagtcgc ttttttcgtt tcttcgccgt cagctgcttt
     2281 ttcagcacgt ttagcttctt cgtgaatgcg gctgtgtgac agaatgatac gttttgcatc
     2341 tttattgaat tcaattactt tgaactgcaa tttttcatcc acctgagcct gtgaaccgtc
     2401 ttctttcacc aaatgttttg gtgtaacaaa accttccact ccgtaaggca atgaaactac
     2461 ggctcctttg tccatcattt ccacgatagt tccttcgtga agactgtcaa cagcaaaaag
     2521 gctttcgagt gcatcccatg gattttcttc gagttgcttg tgaccgaggc ttaaccggcg
     2581 gttgtctttg tcgatttcaa gaacctgaac atcaatgttg gcaccaatct gagtaaattc
     2641 ggatgggtgt ttgattttct tggtccagct aaggtcagaa atgtggatca gaccgtcaac
     2701 accttcttcg atttcaacaa atacaccgaa attggtgaag ttgcgaactt tagcagtgtg
     2761 tttgcttgcc agcggatagc gtgtttcgat attttcccat ggatccgatt tcaactgttt
     2821 gatacccagc gacattttgc gttcgtcgcg gtcgagtgta agaataaccg attcgatttc
     2881 gtcgcctact ttcaggaaat cctgtgcaga acgaagatgt tgcgaccagc tcatttctga
     2941 aacatggatc agcccttcaa cacccggagc aatttcaaca aatgcaccgt aatcggccat
     3001 aacaaccact cttcctttaa ccttgtcgcc aactttgagg tttggatcca gtgcatccca
     3061 tggatgagcc gaaagttgtt tcagacccag agcgatacgt ttcttttcat catcaaaatc
     3121 aagaataact acattcagtt tttgatccag tgaaaccact tcttcggggt gagaaacacg
     3181 tccccatgaa aggtcggtaa tgtgaattaa accatctacg ccgccaaggt cgataaacac
     3241 tccgtaagaa gtgatgtttt taacgacacc ttcgagcacc tgaccttttt gaagtttacc
     3301 gatgatttct tttttctgtt gttcgatttc ttcttcgata agtgctttgt gcgaaacgac
     3361 aacattttta aattcctggt tgattttgat caccttgaat tccattgttt tgttaacaaa
     3421 gatatcgtag tcgcgaatag gtttcacgtc gatctgagaa ccgggcagga acgcttcaat
     3481 tccaaataca tctacaatca taccgccttt ggtacggcat ttaacaaaac ctttaaccac
     3541 ttcctgactt tcaagagcag cattcacacg atcccagcta cgggttgcac gtgcctgttt
     3601 gtgtgaaagg ataagctgac cttttttgtc ttcctgactt tcgatgtaca cttccacttt
     3661 atctcccact ttgagttcgg gattgtaacg gaattcactc atggaaacta ctccgtctga
     3721 tttgaatcct acgtttacta ccacttcgcg tttgttgata gaggttacag taccttccac
     3781 aacttctttg tctttgatag cattcagtgt atcatcgtat attttttcca gttcatcttt
     3841 cgattttcct gtcataacat cccctttttc gtaagagtcc caatcgaaat cggtaaccgg
     3901 tccggttggt tttacatact tgggtgtttc ttttttggca gcgggagctt cttcgattac
     3961 ttcttcggct gcgggtgcag tttcttcagc tacaggagca gtttcttcgg ctacgggtgc
     4021 ttcttcaatc acttcttcgg ctacgggagc agtttcttct gctacagctt gttgtgtttc
     4081 ttctacagct tccggagttt gttcaacttc ttcggcaaat ttttcgtttt cttgcatttc
     4141 tgaaattttg ttttttacta ataaatagta ggttagacat tatgttgatt aaaaaaaata
     4201 gtgggtgcaa aggtaatact tttttattaa acggaaaaac taatttgatt ttagcaaagc
     4261 accaaaccgg ataatttgac aaaaaatact tttaaaataa ttttggttat aaatttgcat
     4321 atcaaaagga taacacccgc cataaaacaa atgggatttc cggtaacgaa aaaagggctg
     4381 aatttcaacc ctttttcccg ttcatttctg attactaaat tcctgtctta ctttgctgtg
     4441 ttttattcca taagtaaagt agattatgaa accgatagcc atccagccca ataatcgtat
     4501 ccaggtatcg agtggcagcg cagccatctg agccagacat acgagtgctc ccaatatggg
     4561 cacaactggc acccagggtg ttcggaaagc ccggtgaaca tgtggttctt ttttacgaag
     4621 gacaatcaga ccgatactta cgatgacaaa tgcaagcaat gtgccaatgg ataccagttc
     4681 gcccagcaga ccgataggaa acaaaccggc aaacagtgca gcaaaacaac cggtaacaat
     4741 gctggtaatg tatggagttt tgaacttagg atgtgtcttt ccgaaacttt tccagagtaa
     4801 tccatcgttg gccatggaat aaaatatacg tgactgagcc agcaggagta ccatcactac
     4861 cgaactcaat ccggctatgg cacctatttt tatgatcggg ctgagccatt ggaggctttt
     4921 accggcagca tcaatggcta cagcaatggg agcagggaca ttcagttcgg tataattaac
     4981 aatgcctgtc attaccagac ttactgccac atatagtatt gtacatacta tgagtgagcc
     5041 cagaatgccg attggcacat cgcgcttggg atttattgct tcctgagcag tggtggaaac
     5101 cgcatcaaaa ccaatgtaag cgaaaaaaac aacagccccg cctgtgagaa tgcccgacca
     5161 gccaaattca gagaatttac ctgtattggc tggtataaat ggcgtccagt tgtccaggtt
     5221 gatgtgagca atgccaaatc caataaaaag caggatgaca gtcaccttaa tcagtacaat
     5281 gatattgttg aaactggccg attctttgat gccgattacc agcaacgtgg tcatcaatgc
     5341 aacgatgaaa acagccggaa aattgataat agctccggta gcatgccacc cactggcatc
     5401 cataatgaac gggctctggc aaatagaagc cggaagatgc agccctaaat cgcccagaaa
     5461 actgctgaca taacccgacc atcccactgc aaccgtagcc gatccgaaca ggtattccag
     5521 aattaaatcc catccgatta tccaggcaat aaactcgccc atggtagcat aaccgtaggt
     5581 gtatgcacta cccgaaacgg gtatcatgga cgcaaactcg gcataacaca atccggccag
     5641 cagacatcca aaggcagaaa caataaacga aatggcaata gctggtccgg catgcagagc
     5701 ggccgcagtg ccggtaagta cgaaaattcc ggcaccaata atgcatccta cgcccaacaa
     5761 aataatattt gtcaggttta gatgtttacg caggctgtct ttgctttctt cggcctgaat
     5821 ttcaatttgc gccagcgatt tgcggatgaa gaggttttta atcataagac tttatattaa
     5881 aaatcaaaca aattgtttct ttcactgtta tatttgttgt tttttcaact ttaaaaagta
     5941 caaatgtaag atttttatta taattcccta atatatgaca aaaaaaagaa tggaaacttt
     6001 tcagggtttc cattctcaga tagtaaagtt aaccgataat attattttat ctagtctttt
     6061 tcgataaatg ccagtgcttc acctttacgc accagttcac cctgcttttt ctcaacaccc
     6121 acaatacgac cgtcacagaa acttccgatt ggctctatgc cgtaattggt ctgaatggca
     6181 cagatcaggt cgaatttttt gaaatgacga ccgtgaggag gttctaccga cacttcgttg
     6241 aagtcgagtt cccacatgag tcttccagaa gcggtggaca caataggttc ggcattcgga
     6301 tatttgagag cacggatatc gggtatttga atctcacttt gagcatattt ttctttgatt
     6361 gcagcttcca gctccttgtc gaaacgttcc tttgccactc ccgatttgaa atcacggtat
     6421 tgtttttcgt gcatggcaaa ttcgaacagc tcttcgtcgt cctgtcctct ctcccagccc
     6481 aattcttcca tttccttgat aaatttaggc agttcatcgg gatagttatc ctgtggattt
     6541 ccttcgaaga attcgtaatt attctttttg gccagttcaa tgatttcggg agccagttta
     6601 cccgggagtt ttccggcttt accaagaatc atatcccagg agtttttatc gatcataccc
     6661 cagcgtggct gtcccttgat catagccatt acattcatga gtgcaatatt tttcacgtac
     6721 tggctgtagg gagttaccag tggcggattg cccagttttg gccagatata acccacttcg
     6781 tcgaacagca tcaccagcat ttcatcagtc gaaagttcgg gtttattatt atttttcagg
     6841 taagtattaa taccggcatg aacgcctttg aggtcggcca tgagcgaacc catcataccg
     6901 cccggcagac cgcaaccgat cagcagtgaa gtcatgtatt tatttctttc gtcaataaaa
     6961 tatccgagga aatcgtccac gaaactttgt gtcaaccggc gggcttccat gtaggctttc
     7021 atgttaattt ccggaacttt aaatcctgca tctttcagca tggcctgaat ggtgatcaca
     7081 tcggggtgca ccatgcccca tgataggggt tccatggcta cgtcgaggta atcgacacct
     7141 gctttggcca cttcgagcat ggaagctacg gagaaacccg gacctgaatg tccgtgatac
     7201 tgaatcggta tttcgggatg cgaggttttg atagctttca ctattttacc aagcatatcg
     7261 ggtcgtccta cacccgccat gtccttgaga caaatttcgt cggcaccttc ggctatgagc
     7321 tgttcagcca tgttcacata atactccacc gtgtgaactt cggaataagt gatacacatg
     7381 gcagcctgag caatcattcc ggcggctttg gcatatttga tggacagggc aatattccgc
     7441 acatcattca atccgcaaaa aatacgggtg atgtccacac cctgcgcttt ttttacttta
     7501 tacatcagcg cacgcacatc ggccggcacg gggttcattc ggatgccatt gagaccacgg
     7561 tctaacatgt gggtctgaat gcccacttca ttgaatggtt ttgtaaatgc acgtaccgat
     7621 ggatttggat tttcaccgta gagcagattt acctgttcgg aagcaccacc attggtttca
     7681 acacgtgcga aacaacccat atcgataatt accggggcga ttttttgtaa ttgatcgatt
     7741 cgtggcacat actttccgct cgattgccac atatctctat aaaccagact aaatttaatt
     7801 tctttcattt ttttaattat tgcaatatga ttttttttgt gtcttttact tctgttattc
     7861 tattgagagc ctttaatggc attattttta gttcgtaata acttgtcaaa tttccgaagt
     7921 tttttccgaa taaaaaaata atattaaaga aatttttcct taataaaaga gcttgaaaga
     7981 ggctgaattt acagtttatc ataaattgtc gcctgaaaaa ttgttttatt caaaatcact
     8041 ggtgagcact tccatttaac tgtaaaaagc cgataaaaac taataaaaat tatttttcgt
     8101 tttttttgag ctatacactt cattaataag ctgattaaat gattttcaac caaactttct
     8161 tatatttgca attgttatta ctattcagta tttattaagc aaatcattta attacacata
     8221 ccatgaaatt tgatacaagc gattttttag taaaacccgg gagtaaagtc aaactcaaag
     8281 agatggacac ccgcttttcg gccggattaa acaagaaaaa aggggaaaaa gaactgctta
     8341 aaaacatcag cgaactggca gaatttcagg aaaaattctg ggcagacaac cgttattcgc
     8401 tgctcatcat attgcaggca cccgatgccg ccggcaaaga cggggctata cggcatgtga
     8461 tgacgggact aaaccctcag agctgccggg tacattgcta caaagtaccg tcgaaagaag
     8521 agctggatca tgattacctg tggcgtcact acaaggattt gcccgaacgg ggaatgatag
     8581 aaattttcaa ccgttcgcat tacgaaaatg tgctggctac aaaagttaat ccgcaatatt
     8641 tgatggctga aaacatcccg ggactggaca agccggagaa agccgataag aaattctgga
     8701 acgaacgata cgaacaaatc aacaacttcg agaagcacat ttatcagaac ggcacgcata
     8761 tcctgaagtt ttttctgaat ctatcgaaag aagagcaaaa agagcgtctt ctggcacgac
     8821 tgaatgaacc tgaaaaaaac tggaaattca gttcggccga ccttacagcg cgtgctcagt
     8881 gggacgagta tcagaaagct tatgaagaga tgatggaaaa aacctctacc gaatatgccc
     8941 cgtggcatgt gataccggca gataagaaat tttttgccag ggtagccatc ggcgaaatca
     9001 ttgtggaaaa actgaaaagt cttgacctgc ggtatcccga aggtgaatcg gccgaagatt
     9061 tgcaaaaagc caaagcgatg ttgatgagtg a
//
LOCUS       DAOPZK010000079         9029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_9115_length_9029_cov_23.269556, whole genome shotgun sequence.
ACCESSION   DAOPZK010000079 DAOPZK010000000
VERSION     DAOPZK010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9115_length_9029_cov_23.269556"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PKH68_06070"
     CDS             complement(<1..1160)
                     /locus_tag="PKH68_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNX12538.1"
                     /translation="MGKVLIIGAGGVGTVVVHKVAQNTEVFTEIVLASRTKSKCDVIA
                     ADVKKRYKVDIKTAQVNADSVADLVALFNEHKPKLVINVALPYQDLTIMDACLLCGVN
                     YLDTANYEPKDEAKFEYSWQWAYQDKFKKAGLTAILGCGFDPGVTSIFTAYAAKHHFD
                     EIHFLDIVDCNAGDHGKAFATNFNPEINIREVTQKGKYYENGKWVETEPHQIHKPLNY
                     PEIGAKESYVIYHEELESLVKNFPSIKRARFWMTFGQEYLTHLRVIQNIGMARIDEVE
                     YNGQKIVPIQFLKAVLPNPGDLGENYKGKTSIGCRIKGFKEGKVKTYYIYNNCSHEDA
                     YRETGTQAVSYTTGVPAMIGAMMFLTGEWTKPGVFNVEQFNPDPFMEQLNLQG"
     gene            complement(1335..2465)
                     /gene="nspC"
                     /locus_tag="PKH68_06075"
     CDS             complement(1335..2465)
                     /gene="nspC"
                     /locus_tag="PKH68_06075"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNX12539.1"
                     /translation="MDYSLIPSPCYVLDEKAFRNNLALIKSVKERAGVEIILAFKAFA
                     MWSVFPIVREYIPCSTASSLAEARLAYEEMGSPAHTYAPAYSGAEFSEIMSYSSHITF
                     NSLSQFYQFYPQTVGKNISCGLRINPEFSDVETDLYNPCAPGSRLGVIVDGLGEQLPE
                     GIEGLHFHTLCESTSYDLEKTLNVVENKFGKYFQQIKWLNMGGGHLMTRSGYDVEHLI
                     KTLQNFKARYPHLQLILEPGSAFAWRTGVLASTVLDVVENHGVKTAMLDVSFACHMPD
                     CLEMPYKPAIIGATDVKSGKPTYRMGGNSCLSGDFYGSWSFDEKLKTGDRVIFEDMIH
                     YTMVKTTMFNGVTHPSIGIWTSENTFRLIRKFGYEDYKNRMS"
     gene            complement(2542..4200)
                     /locus_tag="PKH68_06080"
     CDS             complement(2542..4200)
                     /locus_tag="PKH68_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX12540.1"
                     /translation="MKKIFVPIILFCLLISVTAQNKQNSEYRKFEITKNLSVFNTVLR
                     ELDANYVDSLKYGELVKTGIDNMLMKLDPYTIYMPEEMNDDLKMMTTGEYAGIGALIM
                     QSNGKIIISEPYEGKPAQLNDLRAGDEILEVDGVSTKSKTSSDVSELLKGKNGTEITI
                     KVARSGEKKPLIKKFLRQNIQFNPVSYTSVAGEKTGYLLLDDFTDKAAAEVKSVITDM
                     VKKHQISSLIIDLRDNGGGLVDEAVKIVGYFVPKGTEVVVTKGKSSQMDKTYRTPSEP
                     IFPDMKLAVIVNRSSASASEILAGALQDLDRAVIIGERTYGKGLVQSIRPVGYGGHVK
                     VTTAKYYIPSGRCVQALDYSHRNEDGSVGRIPDSLVSTFKTKNGRLVRDGGGILPDSV
                     TIDSRKLNISYYLLMQNLYFDYATRYAQKHEKIAPAATFRLSDAEFKDFTDFVVNEKK
                     FTYTTQTGKYFNQLLEVAGYEGLDEEAKAEFEALKAKLVPDVKKNMETNRKDIEDMLS
                     TEILKRYYFQKGQIEYTLRDDREIKLAVDMLKNNNQYTKILSGK"
     gene            complement(4226..4678)
                     /gene="coaD"
                     /locus_tag="PKH68_06085"
     CDS             complement(4226..4678)
                     /gene="coaD"
                     /locus_tag="PKH68_06085"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNX12541.1"
                     /translation="MKRAIFPGTFDPFTIGHYNLVMRGLDIFDEIIIGIGVNLSKKTL
                     FDAGKRIDIIRQAFSNEPRVRVMTYDSLTVDFAASVEAKYILRGLRTVSDFEYEHTIA
                     NANRQLNGLETVVLFTETEYSFISSTVVRDLIAYGKDITPYLPPHVKL"
     gene            5040..5318
                     /locus_tag="PKH68_06090"
     CDS             5040..5318
                     /locus_tag="PKH68_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12542.1"
                     /translation="MLSKKFYSNGQPIYELTDDTLTYFFKNGTLKATGPFVNEMMQGE
                     WTFYRETGQLWQVGNFRDNLKHGSFVRYNRSNQVEYQEEFNLGKLIKK"
     gene            5407..6810
                     /locus_tag="PKH68_06095"
     CDS             5407..6810
                     /locus_tag="PKH68_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX12543.1"
                     /translation="MQEHLKDKIFKLIGETADEMQLECYVIGGFVRDIFLHRPSKDID
                     IVVVGSGIELAERLAQKLGKKAKFAVFKTYGTAQVKTKDWEVEFVGARKESYSHDSRN
                     PVVETGTLEDDQNRRDFTINALALCLNKDRFGELVDPFNGLQDLKNFVIRTPLNPDIT
                     FSDDPLRMMRGIRFSAQLRFYLENNTFDAIARNKERINIITKERIADELNKIMLSPKP
                     SVGFILLEKTGLLELIFPELLALKGAETKDGVGHKDNFYHTLAVLDELATHTDNLWLR
                     WAALLHDIGKPRTKKFDRRLGWTFHNHNFIGEKMIPEIFRKMKLPTNEKMKYVQKMVS
                     LHMRPIVLSEDEVTDSAVRRLLFDAGDDIEELMMLCEADITSKNQEKVKRYRQNFQLV
                     RQKLKDIEEKDRVRNFQPPVSGQEIMDTFKLPACAAVGEIKAKIKDAILDGVIPNEYE
                     AAYAYMLKIGKEMNFTE"
     gene            6868..8916
                     /locus_tag="PKH68_06100"
     CDS             6868..8916
                     /locus_tag="PKH68_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12544.1"
                     /translation="MWTKRITGLLIISMLFLSANSRIRQTYITLDYSGSMSGDKYMIS
                     NFTAQLITLLNDRDEVCVIMNGVVKKISGTPDAYKQLRIAQPNVRQQWGDSQYRSQIG
                     DIEAFNRNFRTDPDKNQWLFIIGDGNWNTARYPAVTESFSKITASAGGLKIFYIPYGS
                     SEFYPSDFTTFIRQLRNVRIMNGSTSVRNVFNNSVVLIYYLTSADCQTPQTTIIDRQT
                     AEVTTEIPAKKYLIFYQDNKTEQELPQIISAVSDTVKLNVSLLGKPSTSGLQYKNYQT
                     LLSSALWEVEGKNNKPVSFLTLKFDREIEPGKLKVFIFPDPSFLLKNDSIITVSSRAN
                     TSVPGTKSQESTILLNKSEFTNALKIASGHNNLSESGNVNRPTQTGLRPKITPLKPKA
                     IIYQAPPLTPVTFRKSASQKPGTGYLLYVERNDLINPEKVDISFPVSYNLFFKDIFSW
                     TDEDKLIFLLEPRNGFSQYLMPDTLDLNLIIQTKRNTLSKSATEEEITIKTATAKVNI
                     PVDELLRRLVYGILLIIYLILLSRKARFKKGAMINYLSGNETDHRISVLLRKKGFINW
                     LNRWFNAFVAEKSAIVFEKTGNNITFVARRKRSAIKIHKKDVDSSTMSFRQYAWKDKN
                     YVDLAEGNELIINSTKSDCRKPTVITYDRMKDIRDDVPVFRALLYLVGVVFLLGLFFL
                     "
BASE COUNT         2575 a         1927 c         1906 g         2621 t
ORIGIN      
        1 ccctgcaggt tgagttgttc catgaacgga tcgggattaa attgctccac gttgaaaaca
       61 cccggtttgg tccattcgcc ggtcagaaac atcatggctc caatcatggc gggaacgcct
      121 gtggtatagc ttacggcctg tgttccggtt tcccggtatg catcttcatg gctgcaattg
      181 ttgtagatat agtaagtttt taccttccct tctttaaatc ccttgatacg gcatccgatt
      241 gacgtttttc ctttgtagtt ttctcccaaa tcacccggat tgggcaatac ggctttcaga
      301 aactggatag gaacgatttt ttgtccgttg tattccactt cgtcgatgcg ggccatacca
      361 atattctgaa ttacacgcag gtgagtgagg tattcctgtc cgaaagtcat ccagaaacgg
      421 gcgcgtttga tagatgggaa gtttttcaca agcgattcga gttcttcgtg gtaaatcaca
      481 tacgactctt tggctcctat ttccgggtaa ttcagtggtt tgtgtatctg atgcggttca
      541 gtttccaccc atttgccgtt ttcataatac tttcccttct gagttacctc acgtatgttt
      601 atttcggggt taaagttggt ggcgaaagct ttaccgtgat caccggcatt acaatccaca
      661 atgtcgagaa aatgtatttc gtcgaaatga tgtttggctg catatgcggt aaatatgctg
      721 gttacacccg ggtcgaaacc acatccgagg atggctgtaa gccctgcttt tttgaattta
      781 tcctgataag cccactgcca ggagtattca aattttgctt catccttggg ctcatagttg
      841 gctgtgtcaa ggtaatttac tccacaaagc agacatgcat ccataatggt gaggtcctga
      901 tatggtagtg ctacattaat aaccagtttt ggcttgtgtt cattgaaaag tgctaccaga
      961 tcagctacac tgtcggcatt cacctgcgcc gttttgatat ccactttgta ccgttttttt
     1021 acatcggctg ctattacatc acattttgat ttggtgcggc ttgccaacac aatctcagta
     1081 aatacctctg tgttttgagc tactttgtga actacaactg ttcctacgcc acctgcgccg
     1141 ataatcaata cttttcccat ttttattgtc taataaatta atagataaat ttcattcaaa
     1201 ttctggtcga aaaccgatag gctatttttt cttttttgag agttgcaaat gtacggcaat
     1261 atctcaaacg ttgcaaatta atttgttttt gttttagcca aaaatatatt tttgtattat
     1321 taatatttta ttaatcagga cattctgttt ttgtaatctt catatccgaa ttttcggatc
     1381 aatctaaatg tgttctcaga agtccagatg ccaatggaag gatgggttac tccgttgaac
     1441 atggttgttt tgaccatggt gtaatgtatc atatcttcaa aaatcacccg gtcgcctgtt
     1501 ttcaactttt catcgaacga ccaggaaccg tagaaatcgc ctgacaaaca gctgtttccg
     1561 cccattcgat aagttggttt gccagatttc acatcggtgg caccgattat ggctggttta
     1621 tagggcattt ccaggcaatc gggcatgtgg caggcaaaag aaacgtcgag catggccgtt
     1681 ttaactccat gattttccac cacatcaagt acggtagagg ccagcacgcc cgttctccac
     1741 gcaaatgcgc tgcccggttc gagtatcagt tgaagatgcg gatatcgggc tttgaaattc
     1801 tgtagagtct taataagatg ttccacatca tatccggagc gggtcataag atgtcctcca
     1861 cccatattca gccatttaat ctgctgaaaa tattttccaa acttattttc cactacattc
     1921 agtgttttct ccaaatcata tgatgtggac tcgcaaagtg tatgaaaatg aagaccttca
     1981 attccttcag gcaattgttc accgagtccg tcaacaatca cacccagccg ggaacccggt
     2041 gcacagggat tgtacaaatc ggtttccaca tccgaaaatt cgggattaat gcgcagtccg
     2101 cacgatatat ttttcccgac agtttgcgga taaaactgat agaattgtga cagagaatta
     2161 aatgtgatat gactgctgta actcattatt tcggaaaact cagcaccgga ataagccggt
     2221 gcgtaggtat gtgccggact tcccatttct tcataagcca gacgggcttc ggccagcgaa
     2281 cttgccgttg aacagggaat atactcacgt acgatgggga aaaccgacca catggcaaat
     2341 gccttgaagg caagaatgat ttcaacaccg gctcgttctt ttaccgattt gataagtgcc
     2401 aggttgttac gaaaagcttt ttcgtcgaga acgtaacagg gtgatggtat aagagagtaa
     2461 tccattggaa tataataaat tatttggctt tcagcaatca gcaattagct atcagccatg
     2521 attgattttt gagattattt gttatttccc cgaaagtatt ttggtgtatt gattgttgtt
     2581 tttgagcata tcaacagcca gtttgatttc ccgatcatcg cggagtgtat attcaatttg
     2641 ccctttctga aaataatatc ttttcagtat ttcggtactc agcatatcct caatatcttt
     2701 tcggttagtt tccatgtttt tcttcacatc gggtactaat ttggctttca gggcttcaaa
     2761 ttcggctttc gcttcttcgt ccagtccttc gtagcctgcc acttccagca attggttaaa
     2821 gtatttaccg gtttgggtgg tataggtaaa ttttttctca ttgactacaa agtctgtaaa
     2881 gtctttgaat tcggcatcgc tcaggcgaaa tgtcgctgcc ggtgctattt tctcatgttt
     2941 ctgcgcatac cgtgtagcat aatcgaaata caaattttgc atgagcaggt aataggaaat
     3001 gttgagttta cgcgagtcga tggttaccga atcgggcaga atacctccac cgtcgcgcac
     3061 cagacggccg ttttttgttt tgaatgtcga taccaggctg tcgggaatgc gtcctacact
     3121 tccatcttcg ttgcggtgac tgtaatccag tgcctgcaca cagcgtccac tgggaatgta
     3181 atatttggcg gtagtcactt tgacatggcc accgtaacct acaggccgaa tgctttgtac
     3241 caatccttta ccataggttc tttctccgat aataacagcc cggtcgaggt cctgcagggc
     3301 accggccaga atttcggaag cggaagccga ggagcggttc acgatcacag ccagtttcat
     3361 atccgggaaa atgggttcgg aaggagtgcg ataggttttg tccatctggc tcgactttcc
     3421 tttggtaacg accacttcag tcccctttgg aacgaaatag cctacaatct tcacagcttc
     3481 atccaccaga ccgccaccgt tgtcgcgtaa atcaataatc agggagctaa tctgatgttt
     3541 tttaaccata tcggtaatca ccgatttaac ctcagccgca gctttatctg taaaatcgtc
     3601 gagcagcagg tatcctgttt tttcacccgc aacagaggtg taacttaccg gattaaactg
     3661 aatattctgg cgcaaaaatt tcttaatcag cggtttcttt tcacccgagc gggctacttt
     3721 gatagtaatt tccgtaccgt ttttcccttt cagcaattcg cttacgtccg aacttgtttt
     3781 gcttttggtg ctaaccccgt ccacttccag aatttcatcg ccggcgcgca ggtcattcag
     3841 ttgagccggt tttccttcat agggttccga aatgataatt tttccattgc tttgcatgat
     3901 cagggcaccg atgccggcat attcaccggt agtcatcatt ttaaggtcat cattcatttc
     3961 ttcgggcatg tagatggtgt acggatcgag tttcatcagc atattatcaa tgccggtttt
     4021 caccagttcg ccgtacttga gtgagtccac gtagttggca tccaactccc gcaatacggt
     4081 gttgaatacg ctcaggtttt tagtaatttc aaattttcgg tattcggagt tctgcttgtt
     4141 ttgagctgta accgatatta ataagcagaa taagataatt ggaacaaata tttttttcat
     4201 ttcggatgaa tttcggatgt aaatattata gtttaacgtg aggaggtaaa taaggggtaa
     4261 tgtccttacc gtaagcaatc agatcacgga caacggtgga ggatataaac gaatattccg
     4321 tttcggtgaa tagtaccacg gtttccaatc cgttcaactg ccggttggca ttggcaatgg
     4381 tatgttcgta ttcaaaatcg gatacggtac gaagtccgcg caggatatat ttggcctcta
     4441 ccgaagctgc aaaatccaca gtcaggctgt cgtaggtcat caccctcacc cgcggttcgt
     4501 tgctgaatgc ctggcgaata atatcaatcc tttttccggc atcaaaaagc gtttttttcg
     4561 acagattgac accaataccg ataatgattt catcaaatat atccaatcct ctcattacca
     4621 ggttgtaatg ccctatggta aaagggtcga aagtaccggg gaaaatagct cgtttcattt
     4681 tttagtatct taattaacgg ttgacattcc tgcaatagct gtgccaattg gttgtatgtc
     4741 aaaataatga tttattgcct gaatgatttt aataaatacc gacaaagata gatatttttt
     4801 atttctaaaa gttttaataa atgttgtttc tgaagcgaaa cataatgttg atttggggga
     4861 tagttctttt tgatctgaac tgtcaatctt caatatggag aatatctaaa ctgttttgga
     4921 ttattcctgc attgtgagtt gttgaaaagg ggaatgataa aacaatttta gctattgaaa
     4981 gaatgatatt gtatttttgt tctatttttg tgaaattagc gacttctaaa acatgcctta
     5041 tgttaagtaa aaaattttac tccaacggcc agcccatcta cgaactgacc gacgatacat
     5101 tgacttattt tttcaaaaac ggcacattga aagctaccgg accgtttgtc aatgaaatga
     5161 tgcagggtga atggacgttt taccgggaaa ccggacaact ctggcaggtg ggaaatttca
     5221 gagacaattt gaagcacggt tcctttgtga ggtacaacag aagcaaccag gttgaatatc
     5281 aggaggaatt taacctggga aaattgataa aaaagtagta attctctgtt ccccaaacag
     5341 ctgataatac cgatttttgt ccgtttgaat ttattggatt acttttgcag gagaaacaag
     5401 aaacgtatgc aggagcattt aaaggataaa attttcaaac tgataggcga aactgccgac
     5461 gagatgcagc tggaatgcta tgtaatcggt ggttttgtgc gggatatttt tcttcaccga
     5521 ccgtcgaagg acattgacat tgtggtagta ggcagcggaa tagaacttgc cgaacgatta
     5581 gcccaaaagt tgggaaaaaa agccaaattc gcggtattca agacttacgg aactgctcag
     5641 gtaaagacta aagactggga agtggaattt gtgggtgccc gcaaagagtc ttattcgcat
     5701 gactcgcgaa atccggtagt ggaaaccggc acactcgaag atgaccagaa ccgccgcgac
     5761 ttcaccatta acgctttggc actctgtctc aataaagacc gtttcggtga actggtcgat
     5821 cccttcaacg gattgcagga tctgaagaac tttgtaatcc gaactccgct caatcccgat
     5881 attacctttt ccgacgatcc gctgcgcatg atgcgcggta tccggttttc ggcacagtta
     5941 cgattttacc tcgaaaataa cacgttcgat gccatcgccc gcaacaagga acggataaat
     6001 attatcacca aagaacggat agccgacgaa ctgaataaaa tcatgctttc gcccaaacct
     6061 tcggtcggtt ttatattgct cgagaaaaca ggtttgctgg aacttatttt tcccgaactg
     6121 ctggcgctga aaggtgccga gaccaaagat ggggtgggac ataaggacaa tttctaccac
     6181 accctcgccg tgctcgacga actggccacc cataccgaca acctgtggct tcggtgggct
     6241 gccctgctgc acgatatagg aaaaccacgc actaaaaaat tcgacaggag actcgggtgg
     6301 acatttcata atcacaactt cataggtgaa aaaatgatac ctgaaatttt ccggaaaatg
     6361 aaactgccga cgaacgaaaa gatgaaatat gtgcagaaaa tggtatcgct ccacatgcgc
     6421 cccattgtac tgagtgagga tgaagtaacc gattcagccg tgagaaggct gcttttcgat
     6481 gccggagatg atattgaaga actaatgatg ctttgtgaag cggacattac ctccaaaaat
     6541 caggaaaaag tcaagcgtta ccgccagaat tttcagttgg ttcgccaaaa gctcaaagac
     6601 attgaggaaa aagaccgcgt caggaatttt cagcctcccg taagcggtca ggaaatcatg
     6661 gacaccttta aacttccggc ctgtgctgct gtcggcgaaa ttaaagcaaa aattaaagac
     6721 gccattctgg acggtgtcat tcccaacgaa tacgaagcag cttacgcata catgctcaaa
     6781 attggtaaag aaatgaattt taccgagtaa atgcagcaaa tttagggcta tttcggactt
     6841 ttttcaaaac taaatcacga taaagtcatg tggacaaaaa gaataacagg tttgttgatt
     6901 atcagcatgt tattcctgtc tgcaaactca cgaatacgtc agacttacat cacactggac
     6961 tattcgggca gcatgtcggg cgataaatac atgatttcta acttcacagc acagcttatc
     7021 acactgctca atgaccgcga cgaagtctgt gtgattatga acggtgtagt gaaaaaaata
     7081 tccggcacgc ctgatgctta taaacaactc cgaatagctc aaccaaacgt ccgacagcag
     7141 tggggcgatt cgcagtaccg aagccagatt ggcgatatcg aagcatttaa cagaaatttc
     7201 agaaccgatc ccgacaagaa tcaatggctc ttcatcatcg gcgacggcaa ctggaataca
     7261 gcccgctatc cggctgttac agagagtttt tccaaaatta ccgcatctgc aggtgggttg
     7321 aaaattttct acatacccta tggcagcagt gaattctacc cgagcgattt caccactttt
     7381 atcagacaac tacggaatgt caggataatg aacggctcca cctctgtgcg gaatgtgttc
     7441 aacaacagtg tagtgttgat atattaccta acatcggccg attgccagac gccgcaaaca
     7501 acgattattg accggcaaac agcagaagta accactgaaa taccggcaaa aaagtacctg
     7561 atattttatc aggataataa aacagaacaa gaactgccac aaatcatttc agccgtgagc
     7621 gatactgtga agttgaatgt cagcttgctt ggaaaaccgt caacatcggg attgcaatat
     7681 aaaaactatc aaaccctgtt gagttcagcg ttgtgggaag tggaagggaa aaacaacaag
     7741 ccggtttcat ttctgacatt gaaatttgac agggaaatag aacccggaaa actgaaagtt
     7801 ttcatctttc ccgacccatc tttcctcctg aaaaatgaca gcattatcac cgtcagttcc
     7861 agagcaaata cttcagtgcc aggcacaaaa tcgcaggaaa gtacaatttt actgaacaaa
     7921 agtgaattca cgaatgcgct caaaatagca tccggtcata acaacctatc agaatccggc
     7981 aatgtgaaca gaccgacaca aacaggatta agaccaaaaa taacaccctt aaagcccaaa
     8041 gcgataattt atcaggcccc gccactgacc cccgtaactt tccgcaaatc ggccagccaa
     8101 aaacccggca caggatatct gttatacgtt gagcgcaatg atttaattaa ccccgaaaag
     8161 gttgatattt cctttccggt cagttataat ctttttttca aggacatttt cagttggacc
     8221 gacgaagata aactcatttt tctgcttgag ccgcgaaacg gttttagtca atacctgatg
     8281 ccagacacgc ttgatttgaa tttgattatt caaacaaaaa gaaatacact tagtaaaagt
     8341 gcaactgaag aagaaataac tattaaaact gcaactgcaa aagtaaatat cccagttgat
     8401 gaattgctcc gtcggttagt gtatggaatt ttgttgatta tttatctgat attgctcagt
     8461 cggaaagccc gtttcaaaaa gggtgcaatg atcaattatc tttcaggaaa tgaaacagat
     8521 cacagaattt ctgttcttct gcgaaaaaag ggatttatta actggctgaa tcgctggttc
     8581 aatgcctttg tggccgaaaa atctgcaatt gtttttgaaa aaacaggaaa taacataacc
     8641 tttgttgccc gcagaaaaag atcagccatt aaaatacaca aaaaagacgt tgacagttct
     8701 accatgagct tcagacagta tgcctggaaa gacaagaatt atgttgatct tgccgaaggt
     8761 aacgaattaa tcatcaactc tacaaaatcc gattgtagaa aaccgacagt catcacctat
     8821 gaccgaatga aggatatacg ggatgatgtt cccgttttca gggcattatt gtacctcgtc
     8881 ggggttgtgt ttttattggg attatttttc ctttaacaga aaaacagata gaaggtcgta
     8941 aaaatatcgg gacgctcaaa ctaactgagc tatgtatgag attttcaatg tactttcggc
     9001 tcttttttga ttttatttca ttttccctt
//
LOCUS       DAOPZK010000080         8999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_9176_length_8999_cov_23.518225, whole genome shotgun sequence.
ACCESSION   DAOPZK010000080 DAOPZK010000000
VERSION     DAOPZK010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9176_length_8999_cov_23.518225"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..2614)
                     /gene="lon"
                     /locus_tag="PKH68_06105"
     CDS             complement(188..2614)
                     /gene="lon"
                     /locus_tag="PKH68_06105"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNX12545.1"
                     /translation="MRRNYENLFTNDMMGSSEVFPIISLEDNPTDINLNSGDVLPILP
                     LRNMVIYPGVLVPVSVARPKSLRLIRQAQDNDGLVGACAQIDKKVEDPTIDQLYGTGV
                     VAQIVRVLEMPDNTTTVILEGKKRFQLGEQVSAKPYLKAVVHLMEETLPDTTDKLFVA
                     LTSSIRDLAIKIIENSGIIPQEMAFAIRNIENPVFLINYICSNFGLDVKEKQRLLEID
                     DVIERGYQLLGLLNKESQLLEIKLTIQNKAKEGIDQQQREYFLQQQMKTIQNELGEDS
                     PEQEVENFRNRAKTKKWSEQVAKTFDKELKKLERMSPHAPDYSTQLNYVETMLDLPWG
                     EFTKDNFNLKNAKKVLDRDHFGMDTVKERILEHLAVLKLKGDMKSPIICLYGPPGVGK
                     TSLGKSIATSLNRKYIRMSLGGLHDEAEIRGHRRTYIGAMPGRIMQNIKKAESANPVF
                     VLDEIDKVNADYKGDPSSALLEVLDPEQNGAFHDNYLDIDFDLSKVLFVATANNLNSI
                     PRPLLDRMELIEVSGYTQEEKVEIAIRHLVPKELENHGLKPEMFVLTKPVTNQLIDQY
                     TRESGVRELDRKIAGLMRKVAKNVAIGTKYNAELTPEDLKTYLGAPRYSRDKYEGNDY
                     AGVVTGLAWTSVGGEILFIESSLSPSKSPTLTLTGNLGDVMKESAVLALQYIKAHATE
                     LGIDQKLIDENSVHVHVPEGAIPKDGPSAGITMTTALVSAFTRRKVRNNTAMTGEITL
                     RGKVLPVGGIKEKILAAKRAGITDIVICEENRKDIEEIKNTYLKGLKFHYVTDIMEVI
                     NFALLQEKVK"
     gene            2955..4685
                     /locus_tag="PKH68_06110"
     CDS             2955..4685
                     /locus_tag="PKH68_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNX12546.1"
                     /translation="MKKSYLFLLPIILMLSILFCQNKTNEVTKTYTSRMDSVDFSMAL
                     LEAVNATDSASLSLLNQVYMPKAYSSFWFKNASQFFQRVNLLDSFLQKAPEHGLTAER
                     FDAQKIRTLLAKTDTTNIDYKQLAQLEILLTRSYFKYCSSLEYGCYNPVKLYPNDYFI
                     AMQQPDSAFISRIFSNSDSLVHYLKDVQPVSREYKNLQAELQNMKRLSDSTFKPIPLM
                     PGKEQIKFKIKHPSIPLIARRLMLSGELPFHAKWDSVYTRFDDRILKALNLFRAKNNL
                     IQDNEIGNQTINALNTDFKTRYKIVAINLERSRWKPEKLITNKFAHVNVADMTLRAMR
                     GDTLVHSMRVCVGKPPKHKTPMLYGKMYEVVLNPTWTVPSSIIVNEISHRMTYDPGYL
                     SRNNMKVYKNREEVSAYSVPWGSISKSYQPYVIVQDAGPGNSLGRIKFNFSNPFNVYL
                     HDTNAKSVFQQHYRAVSHGCVRVEKPLELAFFCMNDIDSTNKKQVSKRTILKDQILYS
                     IGREPLTRAGREYVDSVARGKKLSRVELNPGVAVLLDYKTCFTGIKGDVQYCNDHYKM
                     DSLLISKLRP"
     gene            complement(4682..5173)
                     /locus_tag="PKH68_06115"
     CDS             complement(4682..5173)
                     /locus_tag="PKH68_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX12547.1"
                     /translation="MRTSFITEKNEIEEILDGLSTCFVGMNEADGSPYVIPMNFGYLD
                     GNIILHSGPHGKHLDLLARDNRVCVTFCTENNQLMCQHPDVACSYSVASKSVLFKGRI
                     EFVEDMEEKEQLIKAFMKHYIPREFKISAPAIRNVKVWLLRPEKVTAKAFGQNFRNTR
                     SPV"
     assembly_gap    6076..6175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6432..7382
                     /locus_tag="PKH68_06120"
     CDS             6432..7382
                     /locus_tag="PKH68_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNX12548.1"
                     /translation="MSTTTIRLKLSVLSALPKAKKTVIWLLKIIIPISLGVSILQYLG
                     AIDLIAHYLTPLFSYIGLPGEAAIVFITSIFMSLYAPIAILATLPLSPREITILAVMC
                     LITHNMIVETAVQSKTGSNYTVMFLLRVVSSFVAAYFLNLLLPAQVGTAQITESAVVY
                     HSFAEMLLGWGVSSFWLIVKVVLIITGLMILQNILKEFKLLDTLSRSLAPLMSIMGLP
                     RSTSFLWFVAHILGLTYGSAVMIDAVENNELSKTDANYLNFHIAINHSTLEDTLLFAA
                     IGVPVLWMIIPRFVLAILVVWSVRLWYKRKRKLSPVTGNY"
     gene            7466..8059
                     /locus_tag="PKH68_06125"
     CDS             7466..8059
                     /locus_tag="PKH68_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNX12549.1"
                     /translation="MSERISKTKKQLIDVARELFASNGKKNLTMNDIAEASHKGRRTL
                     YTYFKSKDEVYKAVIDNEINLIIDRLILVSKKNINPYEKLKDHILTHFNTVKNSVTRN
                     GTLRADFFRDIYEVERSRRRIDIIELGLIKSILTEGVEKGYFRKMDTEMMSIIILYAM
                     KGLEVPYIRQNLETDYIGEKTDILEFIFNGIKETKDL"
     gene            8243..>8999
                     /gene="lipA"
                     /locus_tag="PKH68_06130"
     CDS             8243..>8999
                     /gene="lipA"
                     /locus_tag="PKH68_06130"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNX12550.1"
                     /translation="MTYLRKPAWLKTNLQSTENFTLVNKIVKENNLHTICTSGRCPNM
                     CECWNRGTATFMILGEICTRACRFCNTLTGKPLPVDPEEPRKVAESIRLMKLKHAVIT
                     SVDRDDLPDKGAAHWAETIRVIKSVNPQLTLEVLIPDFDGDTALIDRVIAEKPEIISH
                     NLETVKRLTSQIRTKAKYETSLSVLKHIASRGATAKTGIMLGLGETEAEVLELMDDAL
                     ATGCSVLTIGQYMQPSRKNIAVSEYIHPDKFAEY"
BASE COUNT         2460 a         1983 c         1875 g         2581 t
ORIGIN      
        1 gtgattagtg attagtgatt agtgattggt gattagtgat cccgaacgtt cgggagtggt
       61 tagtaacagc atcatttata attcataatt cataattcat aattcaagtt agttgctcgt
      121 ttaaaaaaaa gacaggttca tacaattaaa tgtacaaacc tgcctgttgt tgcttttctg
      181 ttgggaatta ttttaccttt tcctgaagta atgcaaaatt gattacttcc atgatatctg
      241 tcacgtaatg gaacttcaat cccttgaggt aggtgttttt tatttcttca atgtctttgc
      301 ggttttcttc acaaatgaca atatcggtaa ttccggctct tttggcagcc aggattttct
      361 ccttgatgcc acccacaggc agcacttttc cgcgcagggt gatttcaccg gtcatggccg
      421 tattgttgcg cactttgcgg cgtgtgaaag ccgataccag cgctgtggtc atggtgatac
      481 ccgccgaagg tccgtctttg ggaatggctc cttctggcac atgaacgtgc acactgtttt
      541 cgtcaatcag tttctgatcg ataccgagtt cagttgcatg agctttgata tactgcaatg
      601 ccagcactgc cgactccttc attacatcac ccagatttcc ggtgagggtg agagtcggtg
      661 atttgctcgg gctcaggctg ctttcgatga agagtatttc gcctcccaca ctcgtccagg
      721 ccaggccggt taccacaccc gcataatcat ttccctcgta tttgtcgcga ctgtaacgcg
      781 gtgctcccag gtaggttttc aggtcttcgg gagttaattc ggcattatat tttgttccaa
      841 tagccacatt ctttgccact ttacgcatca gaccggcaat tttccgatcc agttcgcgca
      901 ctcccgactc acgggtgtac tgatcgatga gctgattagt caccggtttg gtaagaacaa
      961 acatttctgg tttcagaccg tgattttcca gttctttggg caccagatga cgaatggcaa
     1021 tttccacttt ttcttcctga gtgtaaccgc tcacttcgat gagttccatg cggtcgagca
     1081 agggacgcgg aatgctgttc aggttattgg ccgtagccac aaacaagact ttcgacagat
     1141 caaaatctat atcgaggtaa ttgtcgtgaa atgcaccgtt ctgttcggga tcgagcactt
     1201 cgagcaatgc tgatgatgga tcacctttgt agtcggcatt cactttatcg atttcatcga
     1261 gtacaaatac aggattggcc gattcggctt ttttgatgtt ctgcatgatt cgtccgggca
     1321 tggcaccgat gtatgtgcga cgatgaccgc gtatttcagc ttcatcgtgc agtccgccca
     1381 acgacatacg gatatattta cggttgagtg aggtagcaat agattttcca agggaagttt
     1441 ttcccactcc cggagggcca tagagacata taatcggtga tttcatgtcg cctttgagtt
     1501 tcagtacggc caggtgttcc agaatgcgtt ctttgaccgt atccataccg aaatgatccc
     1561 tgtcgagcac cttcttggca ttcttcaggt tgaaattatc cttggtaaat tcgccccagg
     1621 gcaaatcgag cattgtttcc acataattga gctgggtgga gtaatcgggt gcatgggggc
     1681 tcatacgttc gagttttttc agctccttgt cgaatgtctt tgccacctgc tccgaccatt
     1741 tttttgtttt ggccctgttc ctgaagtttt cgacttcctg ctcgggcgag tcttcaccga
     1801 gttcattctg aatggttttc atctgttgct gcaggaagta ttcgcgttgc tgctgatcaa
     1861 ttccctcttt ggctttgttc tggatggtca gtttgatttc gagcaactgc gattctttgt
     1921 tgagtaagcc cagcagctga tatccccgtt cgatcacgtc atcaatttcg agcagccgtt
     1981 gtttttcctt cacatccagt ccgaaattgg aacagatata attaatcaga aagacgggat
     2041 tttcgatatt gcggatggca aaagccattt cctgcggaat aattccggag ttttcgataa
     2101 ttttaatagc taaatcgcgg attgacgagg taagcgccac aaaaagtttg tcggtagtgt
     2161 cgggtaaagt ttcctccatc agatgaacaa cggccttaag ataaggtttt gctgaaacct
     2221 gttcgcccaa ctggaaacgt ttttttcctt ccagaattac ggtagtggta ttgtcgggca
     2281 tttcgagcac cctcactatt tgtgctacca caccggttcc gtatagttgg tcaatggttg
     2341 gatcttccac ttttttatct atctgagcac aggcacccac cagtccgtcg ttgtcctgtg
     2401 cctgccgtat caaccgcagc gatttggggc gcgccaccga taccggcacc agcacacccg
     2461 gatatattac catattgcgc aatggcagga taggcaaaac gtcacccgaa ttgaggttaa
     2521 tatccgtcgg attgtcctcc agcgaaatga tcgggaatac ttcggatgat cccatcatgt
     2581 catttgtaaa taaattttcg tagtttcttc tcatctgtcg atttgtcctt tatttttatt
     2641 cctctgtcag ttctgtcttc aattatctgt caatttggca gtccggagaa atcacttttt
     2701 ccggcacaag ttgaaaaaca ggacataatt aactgtatat cttttattta tatcatttct
     2761 gagataattc gtaccatctt cagttcaatt gttatgccaa agatactaca atatttatga
     2821 atgcaccgga atgtcatttc atggcgtagg tgaaaatatc gattcactcc gttttgaatt
     2881 cccgaaattt cattaccttt gtttttgtta ataatctttt tttcagagta ttaaatattt
     2941 tattcctatt ctttatgaaa aaaagctact tgtttctgtt gcccatcatt ctgatgctgt
     3001 ccattctttt ctgtcagaac aaaaccaatg aagtcacgaa aacctacacc agcaggatgg
     3061 acagcgtgga tttcagcatg gctctgctcg aagcggtcaa tgccaccgat tcggccagcc
     3121 tgtccttact taatcaggta tatatgccca aagcgtattc ctccttctgg ttcaaaaatg
     3181 cctctcaatt tttccaacgg gtgaacctgc ttgattcctt tcttcaaaaa gcaccggaac
     3241 acggcttaac tgccgaacgg tttgatgctc agaaaatccg caccctgctt gccaaaacgg
     3301 atacaacaaa tattgattat aaacagctgg ctcaactgga aattctgctt accaggagtt
     3361 actttaagta ctgcagcagc cttgaatacg gctgctataa tcccgtaaaa ttgtatccca
     3421 acgattactt cattgccatg cagcaacccg acagcgcttt cataagccgg atattctcca
     3481 attccgattc actcgttcat tacctgaagg atgttcagcc agtctcccgg gaatataaaa
     3541 accttcaggc ggaattacaa aacatgaaaa ggctgagcga ctccaccttt aagcccatcc
     3601 ccctgatgcc gggaaaagag cagatcaaat tcaagataaa acacccgtcg atacccctca
     3661 ttgcccgccg gctgatgctg agcggcgaac tgccgttcca tgcgaagtgg gacagtgtct
     3721 atacccgctt cgacgaccgg atactgaaag cacttaatct gttcagggca aaaaacaacc
     3781 tcattcagga caatgaaata ggcaatcaga ccatcaatgc actgaatact gacttcaaaa
     3841 cccggtataa aatagtggct atcaatctgg agcgaagccg ctggaaaccc gaaaagctta
     3901 tcacaaacaa attcgctcac gtcaatgtgg ctgatatgac tttgcgggct atgcgcggcg
     3961 atacgctggt acattccatg cgcgtgtgtg tgggtaagcc accaaaacac aagacaccga
     4021 tgctctatgg aaaaatgtat gaagtcgtgc tcaatcccac ctggacagta cccagcagca
     4081 tcattgtgaa tgagataagc cacagaatga cctacgatcc gggctacctg agccgcaaca
     4141 atatgaaggt gtacaaaaac cgggaagagg tgagcgccta ttcagtgccc tggggcagca
     4201 tcagcaaatc ctatcagccc tacgtgattg tgcaggatgc gggacccggc aattcgctgg
     4261 ggagaataaa gtttaatttt tcaaacccat tcaatgtgta tctgcacgat acgaatgcaa
     4321 aaagtgtatt tcagcagcat taccgggcag taagccacgg ctgtgtgcgg gtggaaaaac
     4381 cactggagct ggcatttttc tgtatgaatg acattgactc caccaacaaa aaacaggtca
     4441 gcaaaagaac catactcaaa gaccagatac tctactccat cggccgggaa ccgctgacac
     4501 gggcaggtag ggaatacgtt gactcggtag cccgcgggaa gaaactgagc cgggtggaac
     4561 taaatccggg agttgcggtg ctcctcgatt ataaaacatg cttcacagga ataaaaggtg
     4621 acgttcagta ttgcaacgac cactacaaaa tggactcact gcttatatcg aaattacgcc
     4681 cttagaccgg cgatctggtg ttgcgaaaat tctgtccgaa cgctttggca gtaacctttt
     4741 cgggacgaag cagccacact ttcacattgc ggatagccgg tgccgaaatt ttgaattcac
     4801 gcggtatata gtgtttcatg aaagccttga tgagttgctc tttttcctcc atatcttcca
     4861 caaattctat ccggcctttg aatagtacgc ttttggaagc cacactgtag ctgcatgcca
     4921 catcgggatg ctgacacatg agttgattgt tctcggtgca aaaggttacg cacacccggt
     4981 tgtcgcgtgc aagcaaatca agatgctttc cgtgaggtcc tgaatgcagg ataatattac
     5041 cgtccaggta tccgaaattc atgggtatga catagggact tccatccgcc tcgttcatac
     5101 ctacaaaaca ggtacttaaa ccgtccagta tttcttctat ttcatttttc tccgtaataa
     5161 aactggttct catctcagtt gggatttttt attggattta attctacaat ataatcgtcc
     5221 gacacaacaa aaaaaacatt aaaaactttt cggcaatggt ataaatgtct ttattgcctg
     5281 ctttttaagt taaatgtgat taatttcggg aaattttctc tcattttatg ttctaaaaca
     5341 tataaaacga tgtatctttg catcgtgttt ttcatggtat tagatttaag gttaattgaa
     5401 gattgggttg tcgtgagaca acctttcttt ttttaaatca aataccaacg ttcacttcgc
     5461 ttcctgttta cggttcggta ctcacttccc gtattacttt cctgcaaaat taaataaatt
     5521 tgcgcttact ttggatttca accaacataa aaactgattg ctttcattca aatctgtgtg
     5581 caaatgccgt ttttaagtta attgtcgtta atttcaagaa attttcttcg attttatgtt
     5641 ctaaaacata taaaacgatg tatctttgca tcgtgttttt catggtatta gatttaaggt
     5701 taattgaaga ttgggttgtc gtgagacaac ctttcttttt tttaaataaa tcctgcaatg
     5761 ctccaaagct ttcaactgct ttcggcaagt caggtttaat cggataaatc tacctcctta
     5821 cccgcagaag ggcaataaag actttgtgtg caaatagttt actcacatcc aagttttttc
     5881 atttcgaaaa aaagctatct ccggattatc tctgatactt cccttttgaa aggaatatta
     5941 taaagaatga gaaacaacag tcttcccggc ctgtaaaaaa aaatgtcgct tatcctgaat
     6001 cctttctgat ataatgactg aatatcggta aaaataaata aatttgcggc cagcataatt
     6061 ttgaattatc agttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatat
     6181 cggtaaaaat aaataaattt gcggccagca taattttgaa ttatcagtta gcggctctac
     6241 aaaggtatct gctgccgaaa actcagtatt ttaagttatt tattgttaat ttcgacaaaa
     6301 taatcgacta aacatgtttt ataacataaa aaacagcgta tctttgcatc gtgtttttca
     6361 tggtattaga tttaaggtta attgaagatt gggttgtcgt gagacaacct ttcttttttt
     6421 aaataaatca gatgtcaacc accaccataa gattaaagct ttccgtgctc tccgctttac
     6481 ccaaagcgaa gaaaaccgtt atctggctgc tcaaaataat tattcccatc agcctcggag
     6541 tgagtattct tcagtatctg ggagcaattg atctgatagc ccactatctg acgccgctct
     6601 tttcgtatat cggcctgccc ggcgaagctg ccattgtgtt catcaccagc attttcatgt
     6661 cgctctacgc ccccattgcc attctggcca cactcccact atcaccccgc gaaatcacta
     6721 ttcttgctgt gatgtgcctc ataacacaca acatgatagt ggaaactgcc gtacagagca
     6781 aaaccggctc caattacaca gttatgtttc tgctacgtgt tgtttcatca ttcgtagccg
     6841 cctattttct gaacctgctg cttccggcgc aggtaggcac tgctcagata accgaaagcg
     6901 ctgttgttta ccattcgttc gccgaaatgt tgctgggatg gggagtcagt tccttctggc
     6961 tgatagtgaa agtggtgctg ataataaccg gattgatgat actgcagaat atactgaaag
     7021 aattcaaact gctggatacg ctttcgcgct cgctggctcc gctgatgagc atcatgggtc
     7081 tgccccgctc tacatccttt ctgtggtttg tggctcacat actgggactt acatacggct
     7141 cggccgtgat gattgatgca gtggaaaaca acgaactgag caaaaccgat gcgaattatc
     7201 tgaacttcca catcgcaata aatcactcca cactggagga cacgctgctc tttgctgcta
     7261 ttggagtacc ggtattgtgg atgattattc cccggtttgt actggcgatt ctggtagtat
     7321 ggtcggtaag attgtggtac aaaagaaaac gtaaattaag cccggtaacc ggaaattatt
     7381 aatattctgt aaaatatttt cacttttttg tacaaaactg tataaaagca gtaatttaga
     7441 gctgagaaaa tctaaacatt aacctatgag tgaacggatt tcaaagacta aaaagcaact
     7501 gatagatgta gcccgggaat tgttcgcatc caatggcaag aaaaatctta ccatgaatga
     7561 tattgccgag gcatcacata aaggaagacg aacgctgtac acctacttta aaagcaaaga
     7621 tgaggtttat aaggcagtga ttgacaatga aataaatttg attatcgaca ggctcattct
     7681 ggtttccaaa aaaaacatta atccatacga aaagttaaag gatcacatcc tcactcactt
     7741 caataccgta aaaaattcgg taacacgcaa cggtacgctg cgggccgatt ttttcaggga
     7801 tatatatgag gtggaacgct cccgccggcg aattgatatc atagaattgg gattgattaa
     7861 aagtattctg acagaaggag tggaaaaagg ctactttcgc aagatggata ccgaaatgat
     7921 gtcgatcatc attctgtatg ccatgaaagg cctggaagtg ccatatataa ggcaaaacct
     7981 ggaaactgat tacattgggg aaaaaacaga tattctggaa tttatattca atggaataaa
     8041 agagacgaaa gatttataaa agttacattc gggcaatcag acaatgttta gccttttttt
     8101 ccattccatg tttcagattc cgcacttgca gagcggtttt tttattctaa ttttgcaaaa
     8161 aaaaggcaaa caaccctgta caggatgaga tctgactttt caatgaacag gggtgctctg
     8221 tgtaaagaat taattttaaa aaatgacata cctccgaaag ccggcatggc ttaaaaccaa
     8281 cctgcaaagt accgaaaact tcaccctggt aaataaaatt gtaaaggaaa acaacctcca
     8341 caccatctgc accagtgggc gttgccccaa catgtgtgaa tgctggaatc gcggcaccgc
     8401 tacctttatg atactggggg aaatttgcac ccgtgcctgt cggttttgca atacgctgac
     8461 cggtaaaccg ctgcccgttg atcccgaaga accgcgaaaa gtggcagaat ccatccgcct
     8521 gatgaagctg aaacatgcgg tgataacttc tgtggatagg gacgacctgc ccgacaaggg
     8581 tgctgcacac tgggctgaaa caataagggt aattaaatcc gttaatccgc aactaacgct
     8641 ggaagtactg atacccgact tcgatggcga tacagcgctg attgaccggg tgattgccga
     8701 aaagcctgaa ataatttcgc acaacctgga aacagtaaag cgcctgacct ctcagatacg
     8761 aaccaaagca aaatatgaaa ccagtctgag tgtattgaaa cacattgcaa gtcgtggtgc
     8821 aacagccaaa accggcatta tgctggggct gggcgaaacc gaagctgaag tgctggaact
     8881 gatggatgac gctttggcga ctggctgctc tgtacttacc atcggtcaat atatgcaacc
     8941 atcgcgcaaa aacatagcgg tgagcgaata cattcatccc gacaaatttg cagaatata
//
LOCUS       DAOPZK010000081         8578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_9809_length_8578_cov_19.518949, whole genome shotgun sequence.
ACCESSION   DAOPZK010000081 DAOPZK010000000
VERSION     DAOPZK010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9809_length_8578_cov_19.518949"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKH68_06135"
     CDS             <1..687
                     /locus_tag="PKH68_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12551.1"
                     /translation="LMWSGSNQYGTVGFNFTPDKDLSQLRAQNYALSMMIKGDSPGAK
                     FQLRFTDTKTAVAGDHPWRMNFTVDETIAAWDNKWHKVFIPLTRFAEGGSWDNGTWYN
                     PTNSFDWKSVDQFSLVAEQAALTGKTFWLDNIQISNLDTARVNTRIIDINSNRTAITV
                     YPNPAVDYTSIQTSEEVHQIILFNISGIPVKKITGSKTVPLGDISPGIYMAFIQYGNQ
                     QFCSKKIIRK"
     gene            935..3394
                     /locus_tag="PKH68_06140"
     CDS             935..3394
                     /locus_tag="PKH68_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX12552.1"
                     /translation="MKTNILIILLTLTSLVSLSGQNHKVRVYGYVIDSNNRGIEDVSV
                     FFEGKNNGTSTNHNGYYDLSITMTDSVTLVYSHLGYQTIRHTILPDQRVVQITVVMPV
                     LVKELAEVNVTSIRRQTSTMETIDPSKYKLMPNSSGSFESMLIAFAGVSSTNELSSQY
                     NVRGGNFDENIVYVNGTEVYRPLLIRAGQQEGLSFINSDMVGNVGFSSGGFNAEYGDK
                     MSSVLDVRYKKPLSFEASASVSLLGASAYVGTAGKRFTQMHGIRYKTSQYLLGTLDTK
                     GEYKPSFIDYQTYLTWQLAPRWEVNFLGNFSQNNFQFTPEERETAFGTYNKAEKLRIY
                     FDGKEKDLFQTAFGALTLYFKPEKDLKLGLTATSFHTNENETYDITGQYVLSELNLDA
                     DTAQQKGAQLGIGTYHEHARNRLKATVASIAHTGEFIRSGHILKWGTTLQREIIADKI
                     SEWEWRDSAGYSMPYRYDKVVDLYYNLKAENTMSSWRTTAYLQDTYKWNNDAALWAVT
                     GGLRANFWTFNREFIVSPRAAISVLPHWDKDFSFRFATGVYYQSPFYKELRDTISDSQ
                     GNVVVQLNKNIKAQRSLHFVLGGDYYFRALGRPFKFTTEAYLKLADRIITYSVDNVRI
                     RYSGENDAKGYTAGVDFKLFGELVPGSDSWINFSLMQSREDVLADSYIEKSSGKTVYP
                     GWISRPSEQRYNFSMLFSDYLPNNPKYKLYLKTIFSDGLPVGPPRSQRYQAVFRTSSY
                     KRLDIGASRVIVNGTDKIMDRAWMKHIKNIWLNVEVFNLFDFRNVNSYYWVSDINKYQ
                     HGVPNFLTGRQLNLKILVDFK"
     gene            3468..4670
                     /locus_tag="PKH68_06145"
     CDS             3468..4670
                     /locus_tag="PKH68_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX12553.1"
                     /translation="MPQTSNRGKSMPESPIRKLVPLADKAKARGIKVYHLNIGQPDLD
                     TPQIGLDAIRNIDRKILEYSPSDGFKSLRIKLASYYRSFNLDVTLDDIIITTGGSEAV
                     SFAFMSCLDPGDEIIVPEPAYANYTAFAIGAGAVVKPVVSSIDEGFALPSIEKFEELI
                     TPRTKGILICNPNNPTGYLYTRSEMNQIRDLVKKYDLYLFSDEVYREFCYTGAPYISA
                     FHLDSIEENVILIDSVSKRYSECGIRIGALVTKNKEVRKNVMKFCQARLSPPLIGQIV
                     AEASMDTPAEYMLDMYNEYVERRKFLIDGLNRISGVYSPIPMGAFYTVARLPIDDSDK
                     FCSWLLSDFEYEGCTVMMAPGTGFYTEPGLGKNEVRIAYVLNKEDLKHALVVLAKALE
                     VYPGRIKQ"
     gene            4880..7249
                     /locus_tag="PKH68_06150"
     CDS             4880..7249
                     /locus_tag="PKH68_06150"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX12554.1"
                     /translation="MKKTGLALFVLTMLCNIIFASPVLTKSKYLISVLSEDGNGAVTP
                     GTVNYPLIYNTDLNNETATDADYWIIKKQADNTYTFQNFASKKYIKHDIYASNDRSAL
                     VLVDAIQTDKSTSFTLELKATSGMSYYIIRSVNNTQKIWNKRTSLSETNYPVGVYSGS
                     GATNELFIFSGADGVPVTDDSFVVKPPFGRQTLGAFTNYADSLKFDLKTPVVDTSKKE
                     FFLTIPESKMGTSFAMNVVFKPRSSSYKLYLGNQLVTSGTAFTFTNVTSLSSFAIEIR
                     DGATVLATGILKFSCLPLVQIYCENTIGWVYNASKLTVTEPDKTDTTELLQMNIKLRG
                     ATAGGKPKKSYALKLKDADGVSSLDRSFLGLRNDNNWILDAMFIDPARMRNRVSTDLW
                     NEFATLPYYSVSEPKLRNGTRGGFVEVFLNDAYNGLYCMTEKIDRKQLNLKKLKYNID
                     STVVTQRGGMYKASDWTFSTLLGKTYWDGRNHSIVNYSNYSDLWNGYENKYPDLGDGE
                     PIEWKPLYDAVNVSSDLNSDANFKANVDATFDLPVFLDYYLFIELMLATDNQGKNTYL
                     SVYDQTVSPKLTITPWDCDGVWGRRWDGSSNLTYANQNFETFLPAYEHSQNNLYLRLL
                     RLNYNGFNTNLRNRYQQLRGSYFSYSSLYNRFSKYSNLFTKSGAASRESSRWSIGSIT
                     SEMNFLSNWITQRLSYLDNQYLGGPYVDTRVNSSTRIRFNISPNPVRDMLTVSGLQSG
                     DRLQLISMQGTILLQKTVDNDNYVIDMSNYSTGIYLIKVNNEMTKVIKK"
     gene            complement(7420..7878)
                     /locus_tag="PKH68_06155"
     CDS             complement(7420..7878)
                     /locus_tag="PKH68_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNX12555.1"
                     /translation="MNAIKRFLATDTQSWSLLIARLALGVVILPHGMQKALGSFGGYG
                     ITGTLGFFESLGLPVFIGFLGILAEFVGAIGVILGAGTRFMAFSIFLMMSGAMIMGGH
                     INNGFFMNWYGAQPGEGIEYFILVLGLALSLIVGGSGKFSVDNLISGKLK"
     gene            complement(7949..>8578)
                     /locus_tag="PKH68_06160"
     CDS             complement(7949..>8578)
                     /locus_tag="PKH68_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNX12556.1"
                     /translation="ANYYNPERVHFGTLRVLNDDWIAGGEGFGKHPHDNMEIITIPLK
                     GGVEHQDSMGNKGVINAGEVQVMSAGTGIFHSEFNANPDTALELLQIWVFSNKKNVTP
                     RYQQMTLADLQKPNELYQILSPSADDAGVWIHQNAWFHLGDLSAGWEGSYTLKNPQSG
                     VYVFIIEGEAAVAGIELSRRDGLGITDAQEIQIAAKSDTKILIMEVPMN"
BASE COUNT         2525 a         1950 c         1782 g         2321 t
ORIGIN      
        1 ctgatgtggt cgggcagcaa tcagtatggc acagtgggct tcaatttcac tcccgataaa
       61 gacctctcac aactcagggc gcaaaattac gccctcagca tgatgataaa aggtgattca
      121 ccgggggcaa aatttcaact tcgctttacc gataccaaaa cggcagttgc cggtgatcat
      181 ccctggcgaa tgaatttcac cgtggatgaa acaatagctg cctgggacaa taaatggcac
      241 aaagtgttta tcccgttaac ccggtttgcc gaaggcggtt catgggacaa cggtacctgg
      301 tacaatccca cgaactcatt cgactggaaa tcagtggatc agtttagtct tgtggcagaa
      361 caagccgctc tcaccggaaa aacgttttgg ctggataata tacagatcag taatttggat
      421 acagcccgtg taaataccag gattattgat attaattcta acaggacagc aatcactgta
      481 tatcccaatc ccgcagtgga ttatacaagc atccagacca gcgaagaagt tcaccagatc
      541 attttattca atatcagcgg catacccgta aagaaaatta ccggaagcaa aactgttccg
      601 cttggtgata tttccccggg catttacatg gcattcattc agtacggaaa ccaacagttc
      661 tgttcaaaaa aaattatccg gaaataattt acctttcttt tagtttcaat tttttccttc
      721 tatcttccat atttatctct tttgaaagat ataactatta atttgttttg attactaatt
      781 tctgttttcc aaattaacaa gtactgtttt tttgcacaaa cggcatatta tcattacttt
      841 tgcaacttat ttaaagtaaa cactgatagt aacatttacc ggtttattcc gtgaatcagg
      901 gatgttacta tattctgcac caagctgtaa tctgatgaaa accaacatac tgatcattct
      961 gttaacattg acttcactgg tcagcctgtc ggggcagaac cacaaagtgc gggtgtatgg
     1021 ctacgtcatc gacagcaata accggggaat tgaagatgtc agtgtttttt tcgaaggaaa
     1081 aaacaacgga acaagtacca accacaacgg ttactacgac ctcagcatca ccatgaccga
     1141 ttcggtcacc ctcgtgtatt cacatcttgg atatcaaacc atccgacaca ccattttgcc
     1201 cgaccagcgg gtggttcaga ttaccgtggt tatgccggtt ctcgtcaagg aacttgccga
     1261 agtcaacgtt accagtatcc gtcgtcaaac ctcgacaatg gagacgattg atccttccaa
     1321 atacaaactc atgcccaaca gcagcggcag ttttgagtca atgctcattg cctttgccgg
     1381 ggtcagttcg accaacgaac tgagctcgca gtacaacgtg cggggtggta actttgatga
     1441 aaacattgtg tatgtcaacg gaacagaagt gtaccggccg ctgctgatac gagccggaca
     1501 gcaggaaggg ctgagtttta tcaactccga tatggtgggg aatgttggat tttcatccgg
     1561 tggtttcaat gccgaatacg gcgataaaat gtcctcggta ctggatgtga ggtacaaaaa
     1621 acccctaagt tttgaagcat ctgcgtcggt aagtttgctg ggagcatcag catacgtggg
     1681 cacagccggt aagcggttta cccagatgca cggcatacgc tacaaaacat cacaatacct
     1741 gcttggcaca ctggatacca aaggggaata caaaccttcg ttcattgatt accaaaccta
     1801 tctgacctgg cagcttgctc cccgttggga agtgaatttt ctgggcaact tctcacaaaa
     1861 taattttcag tttacccccg aagaacgcga aacggcattc ggaacttaca acaaagctga
     1921 aaaactgcga atttattttg atggaaaaga aaaagatctt ttccagaccg cctttggagc
     1981 gctgacgcta tatttcaagc ccgaaaaaga cctgaaactg ggactgaccg ccacttcatt
     2041 ccacaccaac gaaaacgaga cctacgacat caccgggcaa tatgtgctga gcgaacttaa
     2101 cctggatgcc gatacggccc aacaaaaagg tgcacagctt ggcatcggca cctatcacga
     2161 acacgcccgc aaccgcctga aagccacggt ggcaagtata gctcataccg gagaatttat
     2221 acgttcggga catatactta aatggggaac tacgctgcaa cgcgaaatta ttgcggataa
     2281 aattagtgaa tgggaatggc gcgactcagc cggttactcc atgccttacc ggtacgacaa
     2341 ggtggtggat ctgtattata atctgaaagc tgaaaatacc atgagttcgt ggcgaactac
     2401 tgcctacctg caggacacct acaaatggaa caacgatgcc gcactgtggg cagtaaccgg
     2461 tggattgaga gccaatttct ggacattcaa ccgtgaattc attgtgagcc cacgggctgc
     2521 catctcggta ctgccgcatt gggacaaaga cttcagtttc cgttttgcta ccggtgttta
     2581 ctatcagtcg ccattctaca aggaacttcg cgataccatt tccgacagtc agggaaatgt
     2641 ggtggttcaa ctcaacaaaa acattaaggc tcaacgttcg ctgcattttg tgctgggtgg
     2701 ggattattat tttcgtgccc tgggacggcc attcaagttc accactgaag cttatctgaa
     2761 gctggctgac cgcatcatca cctactcggt ggacaatgta cggatacgct attcgggcga
     2821 aaatgatgca aagggttata ctgccggagt ggatttcaaa cttttcggag agctggtacc
     2881 aggctccgat tcatggatca atttttcact gatgcaatcc cgggaagatg ttctcgctga
     2941 ttcatacata gaaaaatcaa gcggaaaaac cgtttacccg ggttggattt cacgtccgag
     3001 tgagcagcgt tataatttct ccatgctgtt ttcggattat ttacccaata atccaaaata
     3061 caagctttac ctgaaaacaa tcttttccga cggacttccg gtaggaccac cgcgcagtca
     3121 gcgatatcag gcagtattcc gaacttcgtc gtacaaacgc ctggatatcg gagcttcacg
     3181 cgtaatagtg aatggaaccg acaaaataat ggatcgagcc tggatgaaac atatcaaaaa
     3241 catctggctc aatgttgaag ttttcaacct gttcgatttc aggaatgtca actcgtatta
     3301 ctgggtatcg gatatcaaca aatatcaaca cggagtgccc aatttcctca ccggacgaca
     3361 actaaacctt aaaattctgg tagatttcaa gtaatacaca aaaaatcagc aggacgtaaa
     3421 aagaaacttt tttaacttgt aaattcaata aaacacgaca aaatactatg cctcaaacat
     3481 ctaatcgcgg gaagtcaatg cccgaatcac ccatccgcaa actcgttcca cttgctgata
     3541 aagccaaggc acgcggcatt aaagtatatc acctgaatat cggtcagccc gacctggata
     3601 ctccacaaat aggactggat gccatccgga atattgaccg aaaaatactg gaatacagcc
     3661 cgagtgacgg cttcaagagt cttcgcatca aactggcatc ttattaccgc agcttcaatc
     3721 tggatgttac gcttgacgac atcatcatta ctaccggcgg ctccgaagcg gtgagctttg
     3781 ccttcatgag ctgcctcgat ccgggtgatg aaatcattgt tcccgaaccg gcctatgcca
     3841 actacaccgc ctttgccatt ggagccggag ctgtagtgaa gcctgtagta tcaagcattg
     3901 atgaaggatt tgcattgcca tccatcgaga aatttgagga actgattacc ccccgaacaa
     3961 aaggcattct gatatgcaat cccaacaacc ccaccggata cctctatacc cgcagcgaaa
     4021 tgaatcaaat ccgcgatctg gtcaagaaat acgatttgta tcttttctcc gacgaggttt
     4081 atcgggaatt ttgctatacc ggagcacctt atatttcggc ttttcacctc gacagcattg
     4141 aagagaatgt aattcttatc gacagtgttt caaaacgtta cagtgaatgc ggcatacgaa
     4201 taggcgcact ggttaccaaa aataaggaag ttcgcaagaa tgtaatgaaa ttctgccagg
     4261 cacggttaag cccacccctc attggtcaga tagtggctga agcctctatg gacactcccg
     4321 ctgaatatat gctggatatg tacaacgaat atgtagaacg cagaaaattt ctgattgatg
     4381 gtttgaatcg tatttcgggt gtgtactccc ccattcccat gggagctttt tataccgtag
     4441 cacgacttcc gattgatgac tcagataaat tctgctcctg gttgctttcc gattttgaat
     4501 acgaaggctg cacggtaatg atggcacccg gtaccggctt ttataccgag cccggtctgg
     4561 gcaaaaacga agttcgcatt gcttatgtgc tcaacaagga agatctgaaa cacgcactgg
     4621 tagtactggc aaaagcgctg gaagtatatc ccggaagaat aaaacaataa cggtttttca
     4681 aacataaaag tcagcaggca atttgcttgc tgactttttt tgatgaaatt ttgactttgt
     4741 aatcaaattt gctgcttcaa actgatctca gtcagccaat cgtatcaggt gaatcagaaa
     4801 atagccgttc atccggttga tatttttaca ttttatccta tctttgcagt gttattctta
     4861 aataaagtca tttaatgcca tgaaaaaaac cggtttagca ctttttgtgc tgacaatgct
     4921 atgcaatatt atttttgcaa gtcccgtact tacaaaaagc aaatacctga tttcggttct
     4981 ttccgaagac ggaaacggtg cagtaacccc tggaactgtc aattatcccc taatatataa
     5041 cacagacctc aacaacgaaa cggcaactga tgccgattac tggattataa aaaaacaggc
     5101 cgataacacc tacacttttc agaatttcgc ttcaaaaaag tatatcaaac atgacattta
     5161 tgccagtaat gaccgctcgg cattggtatt ggttgatgca atccaaactg ataaatccac
     5221 ttcctttaca ttagagctga aagcaacaag tggcatgagc tattacatca tccgctcagt
     5281 aaataatacc cagaaaatat ggaacaaacg gactagcctg tctgaaacaa attatccggt
     5341 cggtgtatat tcaggatctg gtgcaaccaa tgaattattt attttttcgg gtgctgatgg
     5401 cgttccggta acggatgaca gctttgtcgt taagccacct ttcggccgcc aaacgctggg
     5461 tgcattcacc aactatgccg actcactgaa attcgatctg aaaacccccg ttgttgacac
     5521 ttcgaaaaaa gagttttttc tgactattcc agaatctaaa atgggaacca gctttgcaat
     5581 gaatgtggtt ttcaaaccca ggagttcatc ctacaaactc tatctgggca atcagttagt
     5641 gacaagcggc acggctttta cattcacaaa tgttacctca ttatcctcct ttgctattga
     5701 aatacgtgat ggtgcaaccg tacttgctac cggaatattg aaattcagct gcctgccact
     5761 ggtacagatt tattgcgaga acacaatcgg ctgggtgtac aatgcatcaa aactgacagt
     5821 aactgagcct gataagaccg ataccaccga actgctacag atgaatatta aattaagagg
     5881 tgccacagca ggcggaaaac ccaaaaaatc gtacgcactc aagttaaaag acgcggatgg
     5941 tgtgagttcg ctcgaccgtt cattcctggg tctgcgcaat gacaacaact ggatattaga
     6001 cgcaatgttc atagatcctg cacgtatgcg aaaccgtgta tccaccgatc tgtggaacga
     6061 atttgccaca ctcccctact attcagtttc agagccaaaa ctgagaaacg gaacacgggg
     6121 cggatttgtg gaagtatttc ttaatgatgc ctacaacgga ctttattgca tgaccgaaaa
     6181 gatagaccgg aaacaattaa atctgaaaaa actaaaatac aacattgatt caaccgtagt
     6241 tacccagcgt ggtggcatgt ataaagccag tgactggaca ttcagcacct tgcttggtaa
     6301 aacatactgg gatggcagaa accattcaat tgtcaactac agtaattatt ccgatttatg
     6361 gaacggatat gagaacaaat atcctgacct gggtgatggt gaacctatcg agtggaagcc
     6421 tttatatgat gcagtgaacg tttcatccga cctcaacagc gatgcaaatt tcaaggctaa
     6481 tgttgatgca actttcgatc tgcccgtttt tctggattat tatcttttta tagaactgat
     6541 gctggcaacc gataatcagg gtaaaaacac atatctttcg gtgtatgatc agaccgtatc
     6601 acccaaacta acaatcaccc cctgggactg cgatggtgtg tggggaagac gttgggacgg
     6661 atcatccaat ctgacttatg ccaatcagaa ttttgaaaca tttttaccag cctacgaaca
     6721 ttcgcaaaat aatctttatc tgagactgtt gcgtctgaat tacaacggtt ttaacaccaa
     6781 tctgcgaaac cgttaccagc agcttcgcgg ttcatatttc agttacagca gcctctacaa
     6841 ccgtttcagc aagtatagca atctttttac gaagagtgga gccgcaagcc gtgaaagcag
     6901 ccgctggagc ataggcagta taacaagcga aatgaatttc ttatccaact ggattactca
     6961 acgattgagt tatctggata accaatattt aggtggcccg tatgtcgata cccgtgttaa
     7021 ctcttctact agaatccgat tcaatatttc acccaatccg gtacgggata tgctcactgt
     7081 ttcgggtctt caatccggcg acaggctgca actcatctcc atgcaaggaa ccatacttct
     7141 tcaaaaaaca gttgataatg acaactacgt tatcgatatg agcaattata gtactggtat
     7201 ttatctgatt aaggtgaata acgaaatgac gaaagtcata aagaaataag ctcaaaagtc
     7261 cataatgagt gtaaaaggag gctgttatcc gtaaggatgc agtctcttta tttttaatct
     7321 gttaagttaa taatctatta cgttgctgta aattgttact atgcattaat aaaaaaaccg
     7381 atacaagcgc atcggttttt ttattaagat aaaatattgt tatttcagtt tgcctgaaat
     7441 caggttgtcc accgagaatt ttccgcttcc gccaacaatc agtgacaatg caagacctaa
     7501 tacaaggatg aaatattcaa ttccttctcc gggttgtgct ccgtaccagt tcatgaagaa
     7561 gccattatta atgtgtccgc ccataatcat ggcaccgctc atcatcagaa aaatggagaa
     7621 agccataaaa cgtgttccgg ctccgagaat aacaccgatg gcacccacaa actcagccag
     7681 aattcccaga aatccaataa aaacgggtaa tcctaaactt tcgaaaaatc ctaaggttcc
     7741 ggtaattcca taaccaccga atgagccaag ggctttttgc attccgtgag gtaaaatcac
     7801 tactcctaat gccagacggg cgattaataa tgaccagctt tgtgtgtctg tggctaaaaa
     7861 tcttttaatt gcattcataa tttttgtttt ttaatttttg ttttaattta atttcttagt
     7921 tatatattgt tgtttaattt attatcaatc aattcattgg aacttccata attagtattt
     7981 ttgtgtctga ttttgcggca atttgaattt cctgtgcatc ggtaatgccc agtccgtccc
     8041 ttctgcttag ttcgattccg gcaaccgcag cttctccttc aatgatgaaa acgtacacgc
     8101 ctgattgcgg atttttcagg gtgtagcttc cctcccagcc tgccgacaaa tcaccgagat
     8161 gaaaccaggc attctgatgt atccacacac cggcatcatc ggctgatgga gaaagaattt
     8221 gataaagttc attgggtttt tgcagatcgg ccagcgtcat ctgctgatag cgcggtgtga
     8281 cattcttttt gttcgaaaaa acccagattt gaagtaattc gagtgctgta tcggggttag
     8341 cattgaattc gctgtggaaa attccggttc cggcactcat tacctgcact tcaccggcat
     8401 tgataactcc tttgttgccc atgctatcct gatgttctac accgcctttg agtggaatgg
     8461 ttataatttc catgttgtcg tggggatgtt tcccaaaacc ttcgcctccg gcaatccagt
     8521 cgtcgttgag cacgcgcagg gtaccaaaat gcacccgttc agggttgtaa taattggc
//
LOCUS       DAOPZK010000082         8541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_9866_length_8541_cov_20.983267, whole genome shotgun sequence.
ACCESSION   DAOPZK010000082 DAOPZK010000000
VERSION     DAOPZK010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9866_length_8541_cov_20.983267"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1774
                     /locus_tag="PKH68_06165"
     CDS             29..1774
                     /locus_tag="PKH68_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNX12557.1"
                     /translation="MNWNSQEFIWLDWVIIVVGILAVGWAVARSIQKHKRAQQGADSE
                     DFLFGKGEPWYIIGAAIFAANIGSEHLVGLAGTGAKAGVGMAHWEMQGWMILILGWLF
                     VPFYQLMNNKLGKIITMPDFLKNRYTPRTGSWLSIITLIAYVLTKVSVTAFTGGIFLE
                     SLLGLPFWYGAIGLIVLTGIFTVVGGMKGVMTLSAIQTPILIIGSFLVLFLGLAALGD
                     GNIGHGWMAMIDYSKSLSVGADGVAYGTNHMFHFETGDPLYDDYPGFWVFIGASIIGL
                     WYWATDQHIVQRVLGQHKGESSDVVMKRARRGTIAAGYFKLLPVFMFLIPGMIAAALA
                     ARPESGFTLDNPDTAFGSMVKFVLPAGVKGIVTIGFISALIASLAAFFNSCATLFTED
                     FYKPMFKGKTEARYVLVGRIATIVVVILGIVWIPVMMSLGSLYSYLQGIQSLLAPAMV
                     AVFIMGIFSKKITPKAGEMGLIVGFLIGMARLLTNILTDTGKNVMTGGFWDATHWFWH
                     TNWLIFEIWLLVFIMLFMVLVSLFTKKPTAQQIEFITFTDDYKALVTKSWNKWDVIAS
                     LGVVALCAAFYWYFW"
     gene            1845..2534
                     /locus_tag="PKH68_06170"
     CDS             1845..2534
                     /locus_tag="PKH68_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HNX12558.1"
                     /translation="MTNESFYYHSQDSFLVAVDCIIFGFHNNEIKLLVHHRAMEPEQG
                     GLSLMGGFVRKDESVDQAASNILSNLTGLENIFMDQVNVYGELNRDPGERVISCAYYS
                     LIDMNAYDESLLKNHHAFWVSLDKASELIFDHYQMVKDALAILRRKAVLEPIGFNLLS
                     EKFTLTHLQSLYEAIYAKPMDKRNFRKKMLSLGFIDKLEEIDKSGSKKGAYFYKFKDR
                     NLLNNTTNYEF"
     gene            2562..4514
                     /locus_tag="PKH68_06175"
     CDS             2562..4514
                     /locus_tag="PKH68_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX12559.1"
                     /translation="MTKTKFSGLLILLLVAAQLTMAQTHNILVDFKKNGVEIQKTMYG
                     IFFEDINYGADGGLYAELVKNRSFDFPQPFLGWSTFGKVEIKNDGPFENNPNYVRLSY
                     SGHSDKHTGLENEGFFGIAVKQNNEYRFSVWSRKVSNENAGIRIELIDEESNIITSQK
                     LDVNSSDWKQYELILKPNRNYAKSRLRIFLTSKGGIDLEHVSLFPVDTWKGRKNGLRK
                     DLAQALYDLKPGVFRFPGGCIVEGTDLNTRYQWKNSIGPVENRKLNENRWQYTFTNRF
                     FPDYYQSYGLGFFEFFQLSEDIGAEPLPVLSCGLACQFQNKDEHAHAGVGAELDPYIQ
                     DALDLIEFANGSTETKYGKIRAEMGHPEPFNMKLIAIGNEQWGSLYPERLEPFMKAIK
                     ARYPEIKIIGSSGPSPDGKDFEYGWQEMRRLKADLVDEHYYRDPAWFLSGAARYDKYD
                     RKGPKVFAGEYACHPQNRKNNFESALCEAAFMTGFERNADVVHMCTYAPLFAHVQGWQ
                     WRPDLIWFDNMNSVRSVNYYVQQLYGHNAGTNVLNTSENKLPLTGQDGIYASSVYDKN
                     KNEIILKIANTSDAIKTINYNLKGLKNSERAAKKTILKSSGPDMENTLENPTAVAPVT
                     EDMKISGNNFQLNLEPKSFYLLNIQL"
     gene            4859..>5006
                     /locus_tag="PKH68_06180"
     CDS             4859..>5006
                     /locus_tag="PKH68_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNX12560.1"
                     /translation="MKTENTKVRIKDIAVLAGVSEGTVDRVLHNRGEVSKKSKEAVEK
                     VLKEI"
     assembly_gap    5007..5106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5107..5742
                     /locus_tag="PKH68_06185"
     CDS             <5107..5742
                     /locus_tag="PKH68_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX12561.1"
                     /translation="ELKKLEIPFSFIDSMLENADFTTYYGQNSFQSGYLAAKLLLSSL
                     PENSKILTITTNIISAETHQTIVRNAGFKQFILDNNLTGLEQINIEMSSLDESVNMRL
                     LHETFTKHEDIRAAISFNSKVYRFAYYLEAMKMNDIRLIGYDLLQENINYLKSGYISC
                     LLAQRPEKQAYFTVRDMCSKLVLKQQVNKINYMPIDIIMKENIDDYINFNE"
     gene            5780..7027
                     /locus_tag="PKH68_06190"
     CDS             5780..7027
                     /locus_tag="PKH68_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HNX12562.1"
                     /translation="MELSKYSIGVGDRFAHQGVAQLRAIMKANQSGLEISPVWNKSNR
                     EHIYVHTHPADVRREADAAVAALGYRGKYFVDADHINLSTVAPFVETADFFTLDVAAF
                     IGKPSPEADVEAFVNSCEKYLGDLSIPGIEKPLHVTRELLESIASRFLAATEQASEIY
                     KYLVENKGVGNFVTEVSMDEVESPQTPVDMLFILKMLADKGVPAQTIAPKFTGRFNKG
                     VDYRGDLDQFAREFEEDVLVIDFAVKEFGLPKELKLSVHSGSDKFSIYPIMADIIRKY
                     DKGLHLKTAGTTWLEEVIGLAVAGGEALELAKQIYANSFNRKEELCAPYADVIDIDGA
                     QLPSVEEVNTWTSEKYANTLRHIPGNADYNSNFRQLIHVAYKVAAELGETYTSMLEKY
                     AEIIGNCVEENIYDRHLKRLFNL"
     gene            7117..7929
                     /locus_tag="PKH68_06195"
     CDS             7117..7929
                     /locus_tag="PKH68_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX12563.1"
                     /translation="MENLFSVEGKVVVITGGGGILGRGMAEHMCSQGASVVILDRSEE
                     AGNKLANELIAKGYEASYFYCDVLNNEVLEQNSKDIVAKYGRIDVLVNAAGGNMPGAT
                     IGPDSTIFDLQMDAFRKVVDLNLFGTVMPTMVFAKVMVQQKKGSIINISSESALRPLT
                     RVVGYGASKAAVTNFTQYLASELAIKFGEGLRVNAMAPGFFLTEQNRTLMTNPDGSLT
                     ARGQSIIAHTPFGRFGQPEELFGTLQWLASDASKFVTGTVTVIDGGFDVFSI"
     gene            8040..>8541
                     /locus_tag="PKH68_06200"
     CDS             8040..>8541
                     /locus_tag="PKH68_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNX12564.1"
                     /translation="MITLKKDFKYSLLVATSMGVRITPQNGQPVHSSKLFEMQATSAE
                     TNVASISSFLGLPVKVLTTFVKDSPIAQFIKSDLRSRNMDFEGPEVAQGDPWGYRHQF
                     NIADSGFGARGPRVQNDRAGEVGRTLNVKDFDLERIFEKEGVQILHLSGLIGALSHET
                     SNFCLQL"
BASE COUNT         2444 a         1688 c         1890 g         2419 t
ORIGIN      
        1 aaaattaatt tcaaataaaa atttacatat gaattggaat tcacaagaat ttatttggct
       61 cgactgggtc attatagtag ttgggatttt agccgtaggg tgggctgtgg cacgctccat
      121 tcaaaaacac aaacgggctc aacaaggcgc cgacagcgaa gatttcctgt tcggtaaagg
      181 cgagccctgg tacatcattg gtgcagccat ttttgcagcc aatatcggtt ccgaacacct
      241 ggttggtctg gccggaacag gcgcaaaagc aggtgtgggt atggcacact gggaaatgca
      301 aggctggatg atactcatcc ttggctggct ttttgtgccg ttctatcaac ttatgaacaa
      361 taaactgggc aaaattatca ccatgcccga ttttcttaaa aatcgctaca caccccgtac
      421 gggctcatgg ttatcaatca tcacactgat agcttacgtg ctgaccaaag tgagtgtgac
      481 tgcatttacc ggaggtatct tcctggaaag tctcctgggt ctgccattct ggtacggtgc
      541 tatcggcctg atcgtcctga cgggtatctt tacggtagta ggcggtatga agggtgtaat
      601 gacactttcg gccatacaga cacctattct gattattggt tctttccttg tactttttct
      661 tggactggca gcgctcggtg acggcaatat aggtcatggc tggatggcca tgattgacta
      721 ttcaaaatcg ctgagtgtag gtgccgatgg agttgcgtac ggaaccaatc acatgtttca
      781 ctttgaaacg ggtgacccgc tttacgacga ttatcccggt ttctgggtat ttatcggtgc
      841 ttctattatt ggattgtggt attgggcaac cgaccagcac attgttcaac gtgtactcgg
      901 acagcataaa ggtgagtcaa gcgatgtagt gatgaaacgc gcccgcagag gtacgattgc
      961 tgccggttat ttcaaattgt tgcccgtttt tatgttcctc atacccggta tgatagctgc
     1021 ggcactggca gcccgtcccg aaagcggctt tacgctggat aatccggata ccgcttttgg
     1081 ttccatggtt aagtttgtat tacccgccgg tgtgaaaggg attgttacta tcggatttat
     1141 atctgcactg attgcttctt tggcggcgtt cttcaactcc tgcgctaccc tctttactga
     1201 agacttttat aagcccatgt ttaaaggtaa aacagaagcc cgttacgtgt tagttggccg
     1261 tatcgcaact attgttgtag tgattcttgg tattgtgtgg ataccggtaa tgatgagtct
     1321 cggaagcttg tattcttatt tgcagggcat tcaatcattg ctcgctccgg ccatggtggc
     1381 tgtattcata atgggtatat tttctaaaaa aatcacccca aaagcaggtg aaatgggctt
     1441 aatagttggt tttttgattg gtatggcacg cctgctgacc aatatcctga ctgacacggg
     1501 taaaaacgta atgaccggtg gtttctggga tgctacccat tggttctggc ataccaactg
     1561 gctgatcttc gaaatttggc tgctcgtctt cattatgttg ttcatggttt tggtttctct
     1621 gttcacaaaa aaaccaaccg ctcaacaaat cgagtttatc actttcaccg atgactataa
     1681 ggcgcttgta accaagagct ggaataagtg ggatgtaatc gcctctctcg gcgtagtagc
     1741 tttgtgtgct gctttctatt ggtatttttg gtagtttatt atagtagcag gtaaactctg
     1801 atattatttt gtatctgagt ttaccttttg tttaattaaa aaaaatgacg aacgaatctt
     1861 tttactatca ctcccaggat tcatttttag tagcagtgga ctgtatcatt tttggatttc
     1921 acaataatga aataaagctg ttggtacatc atcgtgccat ggaacccgaa caaggcggat
     1981 tatcgctcat gggtgggttc gtacgtaaag acgaatcggt cgatcaggct gcttccaata
     2041 ttctgtcgaa cctcacggga cttgaaaata tttttatgga tcaggtcaac gtatatggag
     2101 aactgaaccg cgatccgggc gaacgggtta tttcctgtgc ttattactcc ctgattgaca
     2161 tgaatgccta cgatgaatcg cttttgaaaa accatcatgc attttgggta agtttggata
     2221 aagcttcaga attgattttt gatcattatc aaatggtgaa agatgcactg gctattttga
     2281 gaagaaaagc tgtactggag ccgatcggtt tcaacctgtt atctgaaaaa ttcacgctca
     2341 cgcatttaca gtctttatat gaagctattt acgccaaacc catggataaa cgaaatttca
     2401 gaaagaaaat gttaagcctt gggtttatcg ataaactgga agaaattgat aaatccggtt
     2461 caaaaaaagg ggcatatttt tataagttta aagaccggaa tttattgaat aatactacta
     2521 actatgaatt ttaattactc ttattactaa tactatttat catgacgaaa acaaaattct
     2581 cgggtttact catattattg ttagttgcag ctcaacttac aatggcccaa acacataaca
     2641 tactggtaga ttttaagaaa aatggtgttg aaatccagaa aaccatgtac gggatatttt
     2701 tcgaagatat caattacggt gctgatggtg gtttgtatgc cgaacttgtt aagaaccgct
     2761 cctttgattt tcctcaaccc ttcttgggct ggagcacgtt cggaaaagtg gagattaaaa
     2821 atgacggacc ctttgaaaat aacccgaatt atgttcgcct tagctattca ggccattccg
     2881 acaaacatac cggacttgaa aatgaaggct ttttcggtat agctgtaaag caaaataacg
     2941 aatacaggtt ctctgtgtgg agccgaaaag tgagtaatga aaatgcaggt attcgcatag
     3001 aacttattga tgaggaaagc aacatcatta cttcgcagaa actggatgta aacagcagcg
     3061 actggaaaca atatgaactg atattgaaac caaaccgcaa ttatgccaaa tcaagacttc
     3121 ggatatttct gacttcaaaa ggtggaattg atctggagca tgtctcactt ttcccggtcg
     3181 atacatggaa aggacgcaaa aacggattgc gaaaagatct ggcacaggca ttgtacgatc
     3241 tgaaaccggg cgtttttcgc ttccccgggg gctgcatagt ggaaggcacc gacctcaaca
     3301 cccgttatca gtggaaaaac tctatcggac cggttgaaaa ccggaaactg aacgaaaatc
     3361 gctggcaata tacatttacc aatcgttttt tcccggatta ttatcagtct tacgggttgg
     3421 gtttttttga atttttccag ttgtcggaag acatcggagc cgagccgctg ccggtgctga
     3481 gctgcggact tgcctgccag ttccagaaca aagacgaaca tgcacatgcc ggggttggcg
     3541 ctgaacttga tccttacatt caggacgcac ttgatttgat tgaatttgcc aacggaagta
     3601 ccgaaaccaa atacggaaaa attcgtgcag agatggggca tcccgaaccc ttcaacatga
     3661 aattaatcgc cattggtaac gaacagtggg gaagccttta tccagagagg ttggagccat
     3721 ttatgaaagc aattaaagcc aggtatcctg aaatcaaaat tatcggttca tccggcccga
     3781 gccccgatgg caaggacttt gagtacggat ggcaggaaat gagaagactc aaagctgacc
     3841 ttgtggacga gcattactac cgcgatccgg cctggttttt atccggtgcg gcacgctatg
     3901 ataagtatga ccgcaagggt cccaaagtat ttgccggtga atatgcctgt catccccaga
     3961 accgaaaaaa caacttcgaa tcggctctgt gcgaggctgc ttttatgaca gggtttgagc
     4021 gtaatgccga cgtggttcac atgtgtacct acgcaccatt atttgcccac gtacagggat
     4081 ggcaatggcg tccggatttg atttggtttg acaacatgaa cagtgtcaga tcggttaatt
     4141 attacgttca gcagctctac ggacacaatg caggtacaaa tgtgctgaac acctctgaaa
     4201 ataagctgcc tcttaccggc caggatggca tttacgcatc ttcggtgtat gataaaaaca
     4261 aaaatgagat tatcctgaaa attgcaaata cttcagatgc gattaaaacc atcaattaca
     4321 acctgaaagg ccttaaaaat tcagaaagag cggctaaaaa aaccattttg aagtcctctg
     4381 gtcctgatat ggagaatacg cttgaaaatc ccactgctgt agcaccggtt accgaggata
     4441 tgaaaatttc ggggaataat ttccagttga atcttgaacc gaaatcattc tatttgctga
     4501 acatacaact ttgattttca gatttatatc aaatctatta aaaaacaggg gagaaatctc
     4561 ctgttttttt tatttaagct catttttgga attttccttt ttactcattg aaaaaaacag
     4621 caatttatct gcctgttttt atgtttgaaa acatattatc ggagatttaa cgaccaacaa
     4681 aagcataaca ccatcgtaat agtctcatta tgctcgtttt acgaattatc attttctcat
     4741 aatcaatttc cgtgttcgca cacattttta tgttttttta cttgatattc caaaatatag
     4801 ttctattttt gcatcaaatt attttcgtgt tcgcacacgt actcattatt ttactcctat
     4861 gaagacagaa aacaccaagg ttagaatcaa agacatcgct gtattggcag gtgtttcgga
     4921 aggtacggtg gaccgggttc ttcacaatcg gggggaagtt tcaaaaaaaa gtaaagaggc
     4981 tgttgaaaaa gtattaaaag agatcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnngagc tcaaaaagct ggaaattcca ttttctttta tcgactcgat gctcgaaaat
     5161 gcggatttca caacttatta tggccaaaat tcctttcaaa gcggatatct ggctgcgaaa
     5221 ttattgctga gttcattgcc cgaaaattca aaaatactga ctattaccac aaacataata
     5281 agtgcagaaa cgcatcagac aatagtacgt aatgcgggat ttaaacaatt tattcttgat
     5341 aataatctga ccggcctgga acagattaat attgaaatga gcagtttgga cgagagtgtg
     5401 aatatgagat tgttgcacga aaccttcaca aaacacgaag atatcagagc tgcaataagt
     5461 ttcaattcaa aggtttatcg gtttgcctac tatctggaag ctatgaaaat gaatgatatt
     5521 cggttaatcg gttacgattt actgcaagag aacattaatt accttaaaag cggatatatt
     5581 tcctgtttac tggcacagcg acccgaaaaa caggcctatt ttactgtgcg tgatatgtgt
     5641 tcgaagctgg tgttgaaaca acaggtaaat aaaataaatt acatgcctat tgatattatc
     5701 atgaaagaaa acatcgacga ttatataaat tttaacgaat aaaataaaca aataaatcaa
     5761 tcctaaacaa tttattatca tggaattaag caaatacagc attggtgtag gcgatcgttt
     5821 cgctcatcag ggagtagccc agcttcgggc tattatgaaa gccaatcagt cggggttgga
     5881 aatcagcccg gtatggaata aatctaaccg tgagcacatc tacgtgcata cccatccggc
     5941 agatgttcgc cgggaagccg atgccgctgt tgctgcactt ggttacaggg gaaaatattt
     6001 tgtggatgct gatcatatta atctgagcac ggtagccccg tttgttgaaa ctgccgattt
     6061 cttcacgctt gatgtagcgg catttatcgg caaaccaagc ccggaagctg atgttgaggc
     6121 atttgtaaac tcatgcgaaa aatacctggg tgatttatcc attcctggta ttgaaaaacc
     6181 gctgcatgtg accagggaat tgctcgaaag tattgcatcc aggtttctgg cagctacgga
     6241 gcaagcttcg gagatttaca aatatcttgt tgaaaataaa ggtgttggca attttgtaac
     6301 agaagtatct atggacgaag tggaaagtcc gcaaactccg gtggatatgc tatttatcct
     6361 caaaatgctt gccgataaag gcgttccggc tcagaccatt gccccgaaat ttaccggacg
     6421 gtttaacaaa ggtgttgatt atcggggtga tttagaccag tttgcccgtg aatttgaaga
     6481 agatgtactt gtcatcgatt ttgcagtcaa agaattcgga cttcccaaag aactgaaact
     6541 gagcgtccat tcgggcagcg ataaattctc catctaccct atcatggccg atattatcag
     6601 gaaatacgac aaaggattgc atttgaaaac cgccggcact acctggctcg aagaagtaat
     6661 cggactggca gtggccggtg gcgaagcgct ggaactcgcc aaacaaattt atgccaattc
     6721 attcaaccgt aaagaggaac tttgcgctcc gtatgccgat gtgattgaca ttgacggtgc
     6781 tcagttgcca tcagtagaag aggtaaatac ctggacgagt gaaaaatatg caaacacact
     6841 gcgccatatt ccgggtaatg ccgactataa ctccaatttc cgacagttaa ttcatgtagc
     6901 ttacaaagtg gctgccgaat tgggtgaaac ctatacctcc atgctcgaaa aatatgccga
     6961 aataattggt aattgtgtgg aagaaaatat ctacgatcgt catttgaaac gattatttaa
     7021 tttgtaaaaa taaatcccaa ataacataga aattcaaact atgaaatccg gaatttgttt
     7081 tacaatcaac gggtaaaata ttaaaaaaat cagaatatgg aaaatctttt tagtgtagaa
     7141 ggaaaagttg tggttatcac cggcggcggt ggcattttag gccgcggaat ggctgaacat
     7201 atgtgcagtc agggtgccag tgttgttatt cttgaccgca gtgaagaagc cggcaataaa
     7261 ttggccaatg aactcattgc taaaggatat gaggcaagtt atttctactg cgatgtactt
     7321 aacaatgaag tattggaaca aaattcaaaa gacatcgtag ccaaatacgg tcgtattgat
     7381 gtgcttgtaa atgccgccgg aggcaatatg ccgggcgcta ctatcgggcc tgacagtaca
     7441 atttttgatc ttcaaatgga tgctttccgc aaagtggtgg atctgaacct gttcggaacc
     7501 gtgatgccaa ccatggtatt tgccaaagtg atggttcagc agaaaaaagg aagtatcatc
     7561 aacatttctt ccgagtctgc gcttcgtccg cttacccgcg tggttggtta cggagcttcc
     7621 aaagctgctg ttaccaattt cactcaatac ctggcttccg agctggctat caagtttggt
     7681 gaagggttac gtgtcaatgc tatggctccg ggctttttcc tcaccgagca aaaccgcacc
     7741 ctgatgacca atccggatgg ttcccttaca gcccgtggtc agtctattat cgctcatact
     7801 ccgtttggcc ggtttggtca acccgaagaa ctgttcggca cactccaatg gcttgccagc
     7861 gatgcatcca aatttgttac cggaacagtt accgttattg acggtggctt tgatgtgttt
     7921 agtatctaat tggcccccta cccccctgaa gggggaaaaa ttgagattaa tataataatt
     7981 accatttaat tcgctcccgg aattcccccc tttagggggt tagggggctg acaaatatta
     8041 tgattacatt aaaaaaagac tttaaatact cgcttcttgt tgcaaccagc atgggtgtac
     8101 gcattacacc gcaaaacggg cagcctgtac atagcagcaa attgtttgaa atgcaggcta
     8161 cgagtgctga aacaaatgtt gccagtatat cttcgttttt aggacttccg gttaaagtat
     8221 tgaccacttt tgtaaaagac agtccgattg cgcaattcat aaaaagcgat ttgcgtagcc
     8281 gcaacatgga tttcgaaggt ccggaagtgg cacagggtga tccctggggt tatcgtcatc
     8341 agtttaatat tgctgacagc ggttttggtg cccgcggacc acgcgtacag aatgatcgtg
     8401 ccggtgaagt tggccgcacg ctgaatgtaa aagattttga tctggaacgg attttcgaaa
     8461 aagaaggggt tcagatctta catttgtccg gtctgattgg tgcattatcg cacgagacaa
     8521 gtaatttttg ccttcaatta g
//
LOCUS       DAOPZK010000083         8160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_10532_length_8160_cov_25.131030, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000083 DAOPZK010000000
VERSION     DAOPZK010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10532_length_8160_cov_25.131030"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..596)
                     /gene="rfbC"
                     /locus_tag="PKH68_06205"
     CDS             complement(48..596)
                     /gene="rfbC"
                     /locus_tag="PKH68_06205"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNX12565.1"
                     /translation="MEIIKTPIEDLLIIKPRIFEDARGYFCESYSEKSFSEAGIDIHF
                     CQDNQSKSGYGVVRGLHYQLNPVSQSKLVSVIVGSVWDVAVDLRKNSPTFGQWYGVEL
                     TAENHLQFLVPQGFAHGFSVLSETAVFSYKCDNFYSPTLERGIAFDDPQLNIDWKIPA
                     EKALISEKDKKHPSFAQAEMNF"
     gene            complement(629..1498)
                     /gene="rfbA"
                     /locus_tag="PKH68_06210"
     CDS             complement(629..1498)
                     /gene="rfbA"
                     /locus_tag="PKH68_06210"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNX12566.1"
                     /translation="MKGIILAGGSGTRLYPVTKSISKQIIPVYDKPMIYYPLSTLMLA
                     GIQEILIISTPQDIVLYENLLGSGEDIGLKLEYAIQPSPDGLAQAFIIGEKFIGTDPV
                     CMVLGDNIFYGFDFSRTLREAATITDGAVVFGYYVNDPERYGVAEFDQNGQVISLEEK
                     PSEPKSNYAVTGLYFYGNDVVEKAKGLKPSKRGELEITDLNRLYLDEKRLSLKLMGRG
                     MAWLDTGTHDSLLEASNFIATIENRQGLQVACLEEIAYRNGYITREQLLKLAEPLKKN
                     NYGKYMIKIANEL"
     gene            complement(1577..2134)
                     /locus_tag="PKH68_06215"
     CDS             complement(1577..2134)
                     /locus_tag="PKH68_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HNX12567.1"
                     /translation="MFIAKKLRQENVCEYLLYMWQIEDLIRALGLDMDRINDQIIKTY
                     PVSDAEKKELYGWYESLADMMRMENVQEKGHLQLNKNLIIQLNDFHHLLLKSGVDAGY
                     AAKFYHILPALNSLRTGLNDGEITDIELCFNFMYGIMVLRMKKAEISAQTLQTQAEIT
                     KFLVLLNKNYLLYSSGELKLEADEV"
     gene            complement(2250..2651)
                     /locus_tag="PKH68_06220"
     CDS             complement(2250..2651)
                     /locus_tag="PKH68_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNX12568.1"
                     /translation="MEVRIDKWLWAVRLYKTRSLATEACKKGKVLIQNVAVKPSRTVK
                     VGEIVQVRQNPVVYSFKVIALAQNRMNAKLVPGFMENVTTPDQLELIELAKIAAQSGR
                     ARGTGRPTKKERRELDEYVEPFFLDGDDGED"
     gene            complement(2781..3344)
                     /gene="pth"
                     /locus_tag="PKH68_06225"
     CDS             complement(2781..3344)
                     /gene="pth"
                     /locus_tag="PKH68_06225"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNX12569.1"
                     /translation="MKYLIVGLGNIGPEYQDTRHNIGFTILDAFAKASNVFFADNRYG
                     ATATVKLKGKTLVLLKPSTFMNLSGNALRYWMQKENIPIERVLVVVDDLALPFGTLRL
                     KPKGSDAGHNGLKNIQEVLGHNEYARLRFGIGNGYPRGGQVDYVLGRWTDEQQAALPP
                     KVDNAGEIIRSFCLSGVELTMTQYNNK"
     gene            complement(3357..3941)
                     /locus_tag="PKH68_06230"
     CDS             complement(3357..3941)
                     /locus_tag="PKH68_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNX12570.1"
                     /translation="MKTFELAGEVRSEVGKKATKAVRSAEAIPCVLYGGKENVHFTVT
                     ESAVRKLVYSPDVFVVNLTVGGVTTTSIMKELQFHPVTDKILHLDFLEISETKPVVVE
                     VPVQLLGLAEGVKAGGKLSLETRKLKVKGLYKNIPEKIDVDVTSVGLGKTVQVGKLQV
                     KDLEILNNKNAVVAQVKLTRAARGAAEAAANASK"
     gene            complement(4110..4850)
                     /locus_tag="PKH68_06235"
     CDS             complement(4110..4850)
                     /locus_tag="PKH68_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNX12571.1"
                     /translation="MKAMIFAAGLGTRLRPLTDSMPKALVPVNGKPLLEHIILKLKEA
                     GFDEIIVNVHHFPDKIIDFLRSKNNFNIRIEVSDEREMLLDTGGAIKKAAYFFDDEKP
                     FLVHNVDILSNLDLKEVYCQHAANPDRLATLVVSHRDTYRYLLFDEQELLCGWVNEKT
                     GETKPGHFSDINAFNKLAYAGIQVLSPKAIQLMNKQEDKFPIMYFYLQNCRSEKIAAY
                     IPENLMMMDMGKTDTLKDADQFAKQNML"
     gene            complement(5109..6017)
                     /locus_tag="PKH68_06240"
     CDS             complement(5109..6017)
                     /locus_tag="PKH68_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNX12572.1"
                     /translation="MSHQNASVKSILFALLANLGIAITKTVAAVITGSGSMLAESIHS
                     YADCGNQGLLFLGLKKSKKAPEVEHPLGHGKEIYFWSFIVALMLFSMGGLFSIYEGIH
                     KLSAHEGLKSPMIAVVVLSVSMVLEAASLYGCITQINKIRHNMSLWQWVKNTRQSELV
                     VVLGEDLAALFGLTFALIAVVLAMITGNPVWDAVGSIGIGALLIFVSFFLAIKVKGLL
                     VGQSADKDTRDEILKFLQNRPEIEEVLNVITLQMGETIMVSIKARMKQVDTAESLVKN
                     INACEAALKTSNKTIQWVFFEPDVEK"
     gene            complement(6203..6385)
                     /locus_tag="PKH68_06245"
     CDS             complement(6203..6385)
                     /locus_tag="PKH68_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12573.1"
                     /translation="MSKDCRGNSPSGAVYGLGFIGAAIYYISHAATFWAGVLGFLKAM
                     VWPALLVYEALKRLAM"
     gene            6575..7237
                     /locus_tag="PKH68_06250"
     CDS             6575..7237
                     /locus_tag="PKH68_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNX12574.1"
                     /translation="MDAERSILYLKKVKSVKPVAPGVFVLSFSRDFNFKSGQVLAIDV
                     VPDRQPRLYSIASGERDEDIDILFDERPDGQLTPILSVLKPGDSIFVSKPFGTFQCHD
                     EKAWWIASGTGIAPFVSMARTGLSTGKVLIHGGRKDENFYFSDILEKLLGQNYIRCAS
                     QHTGTTFYNGRLTQWLRDVRQLPVDGKFYLCGSPEMVVEVRDLLISKGAAFHNIISET
                     YF"
     gene            7241..>8160
                     /gene="rlmN"
                     /locus_tag="PKH68_06255"
     CDS             7241..>8160
                     /gene="rlmN"
                     /locus_tag="PKH68_06255"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNX12575.1"
                     /translation="MDAKQNLFGLTLEQLKIVVRSLDLPAFTATQLADWLYKKHAGVI
                     DEMTNLSKNARRKLEEKYYVGLTLPSNVQTSVDGTKKYLYPTQTKKFIESAYIPDDDR
                     ATLCVSTQIGCKMGCLFCMTGKQGFQGNLTAGEVINQIHSLPEFENLTNIVYMGMGEP
                     LDNIQAVLDSIEIMTAPWGYGWSPKRITVSTIGIIPAMISFLEKSDAHLAVSLHSPFD
                     EERRQIMPIQNVYPLTEVIRELKKWDLGRQRRISFEYIMFKGVNDSPRHARELVRLLN
                     GIKCRINLIRFHSIPDTVLEGTDVAGMVEF"
BASE COUNT         2287 a         1808 c         1654 g         2411 t
ORIGIN      
        1 ccattgtgtc caattttatt ttgttggaca tgggcgtttc atgctgtcta aaaattcatt
       61 tctgcctgag caaacgaggg atgtttttta tctttctccg aaatcagtgc tttttcagcc
      121 ggaattttcc agtcgatatt caactgtggg tcatcaaagg caatgcctct ttccagtgtg
      181 ggcgaataga aattatcaca tttgtaggag aaaacagccg tttcgctcaa taccgaaaat
      241 ccgtgggcaa aaccctgcgg aacaagaaac tgcaaatgat tttcagcagt gagttccaca
      301 ccgtaccact gtccgaaggt gggcgaattc ttacgtaagt ccactgccac atcccatacc
      361 gaacccacta taaccgacac cagtttcgac tggctgaccg gatttagctg ataatgaagt
      421 ccgcgcacca caccgtaacc cgatttggac tgattgtcct gacaaaagtg aatatcaatg
      481 cctgcttcac tgaagctttt ttcgctgtag ctttcgcaga aatatccgcg ggcatcttca
      541 aaaatccggg gtttgatgat aagtaggtct tctatcggtg ttttaataat ttccattatc
      601 gtatagctgc tattttttag taattcagtt ataattcgtt ggcaatttta atcatgtact
      661 tgccgtaatt attctttttg agtggttcgg ccagtttgag aagctgctcg cgggtgatgt
      721 atccgttccg ataggctatt tcttcgaggc aggccacctg caggccctgt cggttttcga
      781 tggtggcgat aaaattggat gcttcgagga ggctgtcgtg ggttccggtg tcgagccagg
      841 ccatgccgcg tcccatgagt ttgaggctca accgtttttc gtccagataa agcctgttta
      901 agtccgtaat ttccaactct ccgcgtttgg atggtttcag tcctttggct ttttccacca
      961 catcattacc gtaaaaatac aaacccgtaa cggcataatt cgattttggt tcgcttggtt
     1021 tttcttccaa actaattacc tggccgtttt ggtcaaattc ggcaaccccg tagcgttccg
     1081 gatcgttgac gtaataaccg aaaacaacag ctccatctgt aattgttgcc gcttcgcgca
     1141 gtgtacgtga gaaatcaaat ccgtagaata tattatcgcc cagcaccata caaaccgggt
     1201 cggtaccgat gaatttctcg ccgataatga aagcctgtgc cagcccatcg ggtgagggtt
     1261 gaatggcata ttccagtttc agacctatat cttcgccgct accaagcagg ttttcataga
     1321 gtacaatatc ctgaggggta gaaataatca ggatttcctg aattccggcc aacatcaggg
     1381 tcgacaacgg gtaataaatc atcggtttat cataaaccgg aataatttgt ttggatatac
     1441 ttttggttac cggatacaac cgggttcccg atccaccggc gagaattatg cctttcatag
     1501 ttttttatgt tttagatatt tcaacaaaga tacatttttt cctttttctt ttatggattt
     1561 gaattgaaat attccgttag acttcgtcgg cttccagctt caattcacca gacgaataca
     1621 aaaggtaatt tttgttcagt aaaaccagaa atttggtgat ttcagcctgt gtttggagtg
     1681 tttgggctga aatttcggct tttttcatcc gcagcaccat gatgccgtac ataaagttga
     1741 aacagagttc aatatcagta atctcaccat cattcagacc tgtacgaaga ctgttcagag
     1801 ctggtaatat gtgatagaat ttggctgcat atcctgcatc cacgccagat ttgagcagta
     1861 aatgatgaaa atcgttgagt tgaatgatca ggttcttatt caattgcaaa tgacctttct
     1921 cctgcacatt ttccatccgc atcatatccg cgagactttc ataccaccca tagagttcct
     1981 ttttttctgc atcactcacc gggtaggttt taattatctg atcattaatc cggtccatat
     2041 ccagcccgag ggcacggatc aaatcttcga tctgccacat atagaggaga tattcacaga
     2101 cgttttcctg tctgagtttt ttggctataa acatttattc ggctataaat tagtacgtat
     2161 caataggtta aatagtgaat agaaagaaat acagagatca caaactgaat attattcgtt
     2221 tgatcgttta atttcaacag taaaagcatt caatcttccc cgtcatctcc gtccagaaaa
     2281 aacggttcca catattcatc cagttctctt ctttcttttt tggtggggcg tccggttccg
     2341 cgtgcccggc ccgattgtgc ggcaattttg gcaagttcga tgagctcaag ttggtcggga
     2401 gtggttacat tttccataaa accggggaca agtttggcat tcatacggtt ctgtgccagc
     2461 gcaatgacct taaatgaata aaccaccgga ttttgccgca cctgaacgat ttcacccact
     2521 ttcaccgtac gcgaaggttt tactgctaca ttttgtatca gcactttacc ttttttacag
     2581 gcctcagtgg ccaggctgcg ggttttgtac aacctcactg cccacaacca tttatcaatg
     2641 cgaacttcca taaagccctc taaatccccc aaaaggggac ttttagtatt attgcgattt
     2701 caaaatttac tcttttcaga tagctgaccc attcggtcaa catgattaag ccattgacac
     2761 agcaaccctg aacatcaaaa ctatttatta ttgtactgtg tcatagtgag ttccacaccc
     2821 gacaggcaaa agctgcgaat tatttcaccg gcattatcca cctttggtgg cagcgcagcc
     2881 tgttgctcat cggtccagcg tccgagcaca taatcgacct gacctccacg cggatagcca
     2941 ttgccaatgc cgaaacgcaa acgggcatat tcgttgtgtc cgagtacttc ctgaatattc
     3001 ttcaatccgt tgtgaccggc atcggagcct ttgggcttga ggcggagcgt accgaacggc
     3061 agtgccagat catcgaccac aacaagtacc cgttcgatgg ggatattttc cttctgcatc
     3121 cagtaacgga gtgcatttcc gctcaggttc ataaacgtgg atggtttcag cagtaccagc
     3181 gtctttccct taagtttaac cgtagctgta gctccatacc ggttgtcggc aaaaaaaaca
     3241 ttggacgcct ttgcaaaagc gtccaatatt gtaaatccta tgttgtggcg ggtatcctga
     3301 tactcgggac caatgttacc cagtccaaca attaagtatt tcattgtcag atagtgttat
     3361 ttcgacgcat tggcagcagc ttcggcagct ccacgggcag cacgtgtcag tttcacctgt
     3421 gcaacaacag catttttatt gttaagaatt tcaagatctt tcacctgaag ttttcctacc
     3481 tgaacagttt tgcccaaacc aacactggtt acatccacat caattttttc aggaatattt
     3541 ttgtagagac ctttcacttt cagtttgcgg gtttcaagac tcagtttacc accggctttc
     3601 acaccttcgg ccaatcccag aagctgaaca ggaacttcaa ctactacagg ttttgtttcg
     3661 ctgatttcga gaaaatcaag gtgcagaatt ttatccgtta cgggatgaaa ctgcaattct
     3721 ttcataatgg aagtagttgt aacacctcct actgtgaggt tcactacaaa tacatcgggg
     3781 gaataaacca gtttgcgtac tgcactttct gtaactgtga aatgtacgtt ttcttttcca
     3841 ccgtaaagta cacaggggat tgcttctgca ctgcgaactg ctttagtcgc tttttttcct
     3901 acttcagagc gaacttcgcc tgctaattca aatgttttca ttttttaatt ataataatgt
     3961 gttactcaaa atttaagcgt ttcgttatta agtgattagt cgcttaattt cccatcacac
     4021 tttctctttc gaaaaaagcg gtacaaaggt agtcagaatc ttttaaatgg acaaacaaga
     4081 ccaattttta atctcagaca gatatgactt tacaacatat tttgttttgc aaactggtct
     4141 gcatctttga gagtgtcggt tttacccatg tccatcatca ttaggttttc ggggatatat
     4201 gctgctattt tctcagaccg gcagttttgc agataaaaat acatgattgg aaatttatct
     4261 tcctgtttat tcattagttg tatcgctttg ggtgacagta cctgtattcc ggcgtaggct
     4321 aatttattaa aggcattaat atccgaaaaa tgtccgggtt ttgtttcacc ggttttttca
     4381 ttgacccaac cgcaaagcaa ctcttgttca tcaaacagca aataccggta ggtatcgcga
     4441 tgacttacca ccagtgttgc gagcctgtcg gggtttgctg catgttggca ataaacttct
     4501 ttcagatcaa ggtttgaaag gatatccacg ttgtgaacca gaaaaggttt ttcatcatca
     4561 aaaaaatatg ctgccttttt tattgcacct ccagtatcca gaagcatttc gcgttcatca
     4621 gaaacctcaa tccggatatt aaaattgttt ttacttctca gaaagtcgat aatcttatcg
     4681 gggaaatggt gtacattcac aattatttca tcaaaacctg cttcttttag cttcagaatt
     4741 atatgttcga gcaaaggttt tccgttaaca ggaaccaacg ctttgggcat actgtcggtg
     4801 agtggtcgaa ggcgcgtacc aagtccggct gcaaatatca ttgctttcat agggtagttg
     4861 agctttttgt cttgtaaaca caaatatatt gaaaaaaaat tgactttagg ttatttttct
     4921 taccgaaaag aatatttcag ccttttggaa taaatcacca caggtagctc gttgtacaaa
     4981 acgacagaag tctgattatc cttatagaac agaaataaat tttcaatcga tattcggtaa
     5041 ttattaatat tgaaaaagag caacctgccg gctactcttt atttagttat atattgaatg
     5101 aaaaatggct atttttccac atccggttca aaaaacaccc attgaatcgt cttgttcgac
     5161 gttttgagag cggcttcaca agcatttata tttttcacca ggctttcggc tgtatccacc
     5221 tgtttcatac gtgctttgat cgataccatg atcgtttcac ccatttgcag cgtgattacg
     5281 ttcagcactt cttctatttc cggtctgttt tgcagaaatt tcagaatttc atcccgggtg
     5341 tctttatcgg cactttgtcc caccagcaat cctttgacct tgatggccag aaaaaaagag
     5401 acaaatatta ataatgcacc aattccgata cttcccacag catcccaaac gggattaccg
     5461 gtaatcatag ccagtaccac tgctatcagt gcaaaagtca acccgaataa agcggccaaa
     5521 tcttctccta aaacaacaac caactcactc tgtcgggtat ttttgaccca ttgccagagc
     5581 gacatgttat gtcgaatttt gttaatctgt gtaatgcaac catacagtga tgccgcttcg
     5641 agcaccatgc tcaccgagag tacaacaaca gctatcatcg ggcttttcag tccttcgtgc
     5701 gcactcagtt tatgaatgcc ttcgtagatg gaaaacaggc cacccatact gaagagcatg
     5761 agcgccacaa taaacgacca gaaataaatc tccttgccat gtccgagcgg atgttccact
     5821 tcaggagcct tcttggattt ctttaacccc aggaaaagca atccctgatt gccgcaatcg
     5881 gcataggagt gaatggactc ggccaacata gaaccagacc cggtgatgac tgctgcaacg
     5941 gttttggtga tggcaatccc cagattagcc agcaaggcaa aaagtattga ttttacggaa
     6001 gcgttttgat gtgacatgtt gctattttac aagttggaat gaagaaaaaa taaggctgca
     6061 aaaataaaac tctttttgaa gataaataac gagtaataat gatttttcag taaaggtgtt
     6121 gcttaaatgc ataaaacacc tttcccggca attcaattcc gggtaaaaaa aaagattgac
     6181 aacgaactgc ttaaagaaca acttacattg ctaatctttt caatgcttca taaacgagca
     6241 gtgccggcca aaccattgct ttcagaaagc ccaacacacc agcccagaag gttgcagcgt
     6301 gtgaaatata ataaatggct gctcctataa atcccaatcc gtaaacggct cccgacggac
     6361 tgtttcccct acaatctttt gacatttctt gtatttatta atattaaaag aaaaaccgga
     6421 aaaaaggaat taataacccg ccaaagataa acaatcatcc cgaaaacttt ttatttttct
     6481 tcataatttt acggaaagaa aaaatacttt ttttcttagc gttcagaaca ctccggacgt
     6541 ttgggttgtt attacatgat agcaaaaaaa gtgtatggat gctgaaagat caatattata
     6601 tctcaaaaag gtaaaatcgg taaaaccggt tgctccgggt gtttttgtgc tctccttttc
     6661 acgcgatttt aatttcaaat ccggacaagt gctggcaatt gatgtggtac ccgacaggca
     6721 gccacgcctc tacagcatag ccagcggcga aagggatgaa gacatcgata ttttgttcga
     6781 tgaaaggccc gacggacaac ttaccccgat attatcagta ctaaaacccg gcgacagtat
     6841 tttcgtttcg aaaccattcg gaacatttca gtgtcacgat gaaaaagcct ggtggatagc
     6901 ctcgggaaca ggtattgcgc cctttgtttc catggcacga acagggctgt caaccggcaa
     6961 agtactcata catggaggca gaaaagatga aaatttctac ttttcggata tactggaaaa
     7021 actattagga cagaattaca ttcggtgtgc atcacaacat acaggaacga ctttctacaa
     7081 cggaagatta acacaatggc tccgtgacgt caggcagtta cccgtggatg gaaaattcta
     7141 tctttgcggt agtcccgaaa tggtggtgga agtgagggat ctgctgattt caaagggtgc
     7201 agcctttcat aatataattt ctgaaactta tttttaatta atggacgcca aacaaaatct
     7261 tttcggactg acactggagc aactgaaaat agttgtacga tcgcttgatt tacccgcatt
     7321 tacggcaaca cagctggctg actggttgta taaaaaacat gccggcgtca ttgatgaaat
     7381 gaccaacctc tcgaaaaatg cacgccgtaa gctggaagaa aagtattatg tcggtttgac
     7441 ccttccttcc aacgttcaaa cttcggtgga tggcacaaaa aaatatctct atcccacaca
     7501 aacaaaaaag tttatcgaat cagcttatat accggatgac gatcgggcta cactttgtgt
     7561 gtctactcag ataggctgta aaatgggatg tctgttctgc atgaccggta aacagggttt
     7621 tcagggaaat ctgacggcag gagaagttat caatcagata cattcgctgc ccgagtttga
     7681 aaatctaacc aacattgtgt atatggggat gggtgagccg ctcgacaata tacaggccgt
     7741 actcgacagc atcgagatta tgactgctcc gtggggttat ggatggagcc ccaagcgaat
     7801 taccgtatca acaattggaa ttattcccgc tatgataagt tttctggaaa aaagcgatgc
     7861 acacctggca gtcagcctgc attcaccatt cgacgaagag cggcggcaaa tcatgcctat
     7921 tcaaaacgta tatccgttaa cggaggtgat ccgggaactg aaaaaatggg atttgggtcg
     7981 tcagcggcgt atttcattcg aatatattat gttcaaagga gtgaacgaca gtcctcggca
     8041 cgcccgtgaa ctggttcggc tgctcaacgg catcaaatgt cgcataaacc tgatacgttt
     8101 tcattccata cccgataccg tccttgaagg aactgatgta gccggaatgg tagaatttca
//
LOCUS       DAOPZK010000084         8105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_10620_length_8105_cov_21.864969, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000084 DAOPZK010000000
VERSION     DAOPZK010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10620_length_8105_cov_21.864969"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1233)
                     /locus_tag="PKH68_06260"
     CDS             complement(<1..>1233)
                     /locus_tag="PKH68_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX12576.1"
                     /translation="TDKEQKNLFVLSSKKMQKMEISSEKFTPVDYKAEMKLDLAQERE
                     FMFNTVYREEKERFYTTTMHGVNWEKLTDNYRKFLPHINNNYDFSEMLSEMLGELNVS
                     HTGSGYRASAGDDKTAELGLFVSRNGNKNGLLIDEILINGPFDDFRSKVKTGDVIEAI
                     DGNEIASDTDYFPLLEGKAGKNTLISIYSPATGKRWDEVIKPIAASTLDNLLYNRWVK
                     QREAEVERLSNGRLGYVHIPSMGDESFRKVYANALGKYYKKEGMVIDIRYNGGGRLHE
                     DIETFFSGKQYLMQEVRGKDYSEMPSRRWNKPSVMLMTEADYSNAHGTPWVYKTMKMG
                     KLVGMPVPGTMTSVNWVTLQDPSLYFGIPVVGYRTAEGTYLENSQLEPDIKAPLDLTK
                     AVAGEDTQLEAAVKALLK"
     assembly_gap    1234..1333
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1334..3130)
                     /locus_tag="PKH68_06265"
     CDS             complement(<1334..3130)
                     /locus_tag="PKH68_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HNX12577.1"
                     /translation="MNKKIVLSGLFLLSCLFINAENPLWMRYPAISPDGSQIAFSYQG
                     DIYKVAVEGGQAARLTTNNAFDSNPIWSPDGQKIAFVSDRENSNRDIFIMSASGGTAK
                     RLTTHTSTEKPYTFTPDGKYIVYSAHYQDPVKSALFPTSMLSELYKVSVEGGRPEMIL
                     ASPAEKINFNKAGSKFLYQDLKGFENTWRKHHTSSVTRDILEYDIKTGTHRKLIDRPG
                     EDTDPVYSADGNSFYFLSEKSGSYNVFSASLNNPTDQKQLTTFSDNPVRFLTAANDGT
                     ICFGYDGEIYTLKKGAAKPQKLALNITNDLDGEQEKKFSFSTGATSAVNSPDGKQIAL
                     IVRGEVFVSSVGYTTTKQITKTVAEERDVDFGADNRSLVYASYRDGYWNIYRAKIGRK
                     EDPNFPNATTIEEEVLIKNDKSEKQHPKFSPDGKEVAFVQDRAKLMVYNIESKKLRQI
                     TDGSFQHESDGDMTFEWSPDSKWFALEYVSNNHAPYSDIGIVSAAGGAEVFNITGSGY
                     TNHKPRWVMDGNAILFNSEQYGMRNHASWGSMEDVMMVFVNREAYNKYKMNKEEYELF
                     TDAEKEVKKAEEDAKKKAEEAKKGKEDTKDKKK"
     gene            3293..4363
                     /locus_tag="PKH68_06270"
     CDS             3293..4363
                     /locus_tag="PKH68_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HNX12578.1"
                     /translation="MKKLLFFLFLSFFSFSSAQVRNEINIPDIMGYKTLKCDFHMHTV
                     FSDGLVWPTVRVSEAYTEGLDAIAITDHIEYRPHKSDMPAASHNRSYELAEASAIASG
                     ILLIRGSEITRAMAPGHSNALFLSDCNALDVPAWQDAFKAAKAQNAFIFWNHPGWDAQ
                     QPDTTRWFKEHTELYNAGLMQGIEVVNGPEYFPEAHRWCLEKKLTMLGNTDVHEPLAF
                     KDGKHRTMTLVFATERTQEAIKDALINRRTVVYLDDKLIGEERYLNALFHGSIEILKT
                     EKTSTQYRITVKNNSDIPFYLHKTSHNPDLVYFRDYTLEPHSQHTITVKLTKGITGGD
                     VNFEITNLLVEPGKGLQIRFPI"
     gene            4520..5113
                     /locus_tag="PKH68_06275"
     CDS             4520..5113
                     /locus_tag="PKH68_06275"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNX12579.1"
                     /translation="MDLSELLNSSIGQSIVKSVAGQFGLDESQAASAVNTAVPVILGG
                     LNKNAQTPEGAESLNSALESKHDGSLLDNLGGLLSGNVGDLLQDGGGILGHVFGDKVG
                     AVENGVAQKTGISTDKIGPIISMLAPIVMAYLGKQKSQSNVSAGGLGDLLGGLLGGGG
                     SAQSSGGGGLMDMVTGLLDKDKDGSALDDILGMFTKR"
     gene            complement(5210..6886)
                     /locus_tag="PKH68_06280"
     CDS             complement(5210..6886)
                     /locus_tag="PKH68_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HNX12580.1"
                     /translation="MEWLNSLLFGEGIAHTILIYSFVIFTGVLLGKIKFWGISLGTTF
                     ILFMGILVGHMGYTINHEVLDFLKDFGLILFIYSIGLQVGPGFFSSFKKGGMVLNLLA
                     AGIVLLGVTVAVSFYFVLNGRVEMPMIVGIMSGAITNTPGLGAAQEALRQAHEAGQIA
                     EIPQIALGYAVAYPLGVVGIISSIILIRLIFRINLEKEAKALEQDEDTAMEKPERITL
                     KLTNNAISGKTLHQVKTMVGRNFIISRMMSNGIYSIPQSETILKNGDILLVISALADT
                     ETITAFIGEPAELDWKDSEKHLVSRRIVITHSKINGKTLGSLRLRTLFGVNITRVNRS
                     GIDLLGATNLTLQVGDRVMVVGELENINKVEKLLGNTLKRLNEPPIVTIFVGIFLGIL
                     FGSIPFFIPGMPMPVKLGLAGGPLIIAILIGRFGYKLKLITYTTQSANLMLREVGISL
                     FLASVGLAAGGKFVETVFTGQGLLWVGIGFAITVLPLLIIGFIGRKFAKLNYFTLIGL
                     LAGSTTDPPALAYSSSISSNDQPAVAYSTVYPLTMFLRVIMAQLIILAFI"
     gene            complement(7137..7994)
                     /locus_tag="PKH68_06285"
     CDS             complement(7137..7994)
                     /locus_tag="PKH68_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNX12581.1"
                     /translation="MNTKIPSYLYQKGNIIRLIIFTAIFALLFINFFQPFGSRGWYPQ
                     ISDFKYFVFSSLIILTGMLVVVISRMIMLHYTRRHNLNYWQFAGWILCEVLAMSLFYA
                     LFTKFIPREGMNRDFMEIFRKSTINTGLVLLLPYSISWLYFSWKEKNNMLENIEKEDT
                     VLENHSKSMIAFPDEKGELKISIMQENILYIDSADNYITLHYLNKSKLSHYMLRNTLK
                     WMDENLIKDTLLVRSHRSYIVNLDKVKVLRKTKTGIFIELDAENTPDIPVSKTYYERF
                     MNKFSQYSV"
BASE COUNT         2210 a         1731 c         1715 g         2349 t
ORIGIN      
        1 tatttcagca atgcctttac ggctgcttcc agctgagtat cttcacctgc aacggctttg
       61 gtgaggtcaa gaggcgcttt gatgtcgggc tccagctgac tgttttcgag gtatgtgcct
      121 tcggccgtgc gataaccgac caccggaatt ccgaaataca acgatggatc ctgcaacgta
      181 acccagttga cactggtcat ggttcccggt acgggcattc ccacgagttt acccattttc
      241 atggttttat acacccacgg tgtcccgtgc gcattggaat aatcggcttc ggtcatcagc
      301 atcaccgaag gtttgttcca tcggcggctg ggcatttcgg agtagtcttt gccgcgaact
      361 tcctgcatca ggtattgttt tccgctgaaa aaagtttcaa tatcttcatg caaccggcca
      421 cctccgttgt agcggatgtc aatcaccatg ccttcttttt tataatattt accaagggca
      481 ttggcatata ctttgcggaa actttcatcg cccatcgaag gaatatgtac ataccccagg
      541 cgaccgttcg acaatctttc cacttcagct tcccgctgtt ttacccagcg gttgtaaagc
      601 agattgtcga gtgttgaggc agctattggt tttatgactt catcccagcg cttgccggta
      661 gccggagaat aaattgaaat cagggtgttt tttcccgctt ttccttcaag cagggggaaa
      721 taatcggtat ccgatgcaat ttcgttgccg tcaatagctt cgatcacatc accggttttc
      781 actttggagc ggaaatcatc aaatggtccg tttattagga tttcatcaat cagtagtccg
      841 tttttattcc catttctgct cacgaacaga cccagttcgg ctgttttgtc atcgcctgcc
      901 gaagcccgat agcccgagcc ggtgtgcgaa acattcagtt cacccagcat ttcgctcagc
      961 atctccgaaa aatcgtaatt attattgata tgaggcagaa atttccggta gttgtctgtc
     1021 agtttttccc agttaactcc gtgcatggta gttgtataaa aacgttcttt ttcttcgcgg
     1081 tacacggtat tgaacatgaa ttcgcgttcc tgagccagat ccagtttcat ttccgctttg
     1141 taatcaaccg gcgtgaattt ttcggacgaa atttccattt tctgcatttt tttactgctc
     1201 agtacaaaca ggttcttctg ttctttgtcc gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnttttttc ttgtctttag tatcctcttt gccttttttg gcttcttccg
     1381 cttttttctt tgcatcttct tcggcttttt tgacttcttt ttcagcatca gtgaagagtt
     1441 cgtactcttc tttgttcatt ttgtatttgt tgtatgcctc ccggtttaca aaaaccatca
     1501 ttacatcttc catggagccc cacgaagcat ggtttcgcat accgtactgt tcggaattaa
     1561 atagaatggc attcccatcc attacccagc ggggtttgtg attggtgtag ccgctgccgg
     1621 ttatgttgaa aacttcagca cctcctgcag cactcacaat gcctatatcg gaataaggtg
     1681 cgtggttgtt ggatacatat tccagtgcaa accatttact gtcgggtgac cactcgaaag
     1741 tcatgtcacc gtcgctttcg tgctgaaagg atccgtcggt gatttgacga agttttttac
     1801 tttcaatatt gtaaaccatc agtttggcac ggtcctgaac aaaagctact tctttgccgt
     1861 caggtgaaaa ttttggatgc tgtttttcgg atttgtcgtt tttgatcagc acttcctctt
     1921 caatagtggt tgcattgggg aaattcggat cttctttgcg cccgattttt gccctgtaaa
     1981 tattccagta accatcgcga tagctggcat ataccagcga acgattatcg gcaccgaaat
     2041 ccacatctct ttcttcagcc acggttttgg ttatttgttt ggtggtagtg taacccaccg
     2101 aacttacaaa aacctcaccg cgcacaatca gtgctatttg ttttccatcg ggagaattta
     2161 ccgccgaagt ggctcccgta ctgaaactga atttcttttc ctgttctcca tccagatcat
     2221 ttgtgatgtt taaagccagt ttctgaggtt tcgcagctcc ttttttcagc gtatagattt
     2281 caccgtcgta tccgaaacaa attgttccgt cattggctgc cgtcagaaat cgaacgggat
     2341 tgtcgctgaa agttgtcaac tgtttctggt cagtcggatt gttcaacgag gccgaaaata
     2401 cattgtaact gccgcttttt tcactcagga aataaaacga attgccgtcg gcactgtaaa
     2461 caggatcggt atcttcgccc gggcggtcta tcagtttgcg gtgagttccg gtttttatgt
     2521 catattccag tatatcgcgg gtcaccgacg aagtgtggtg tttacgccat gtgttctcga
     2581 aacctttcag atcctgatac agaaatttgc tgcccgcttt gttgaaattg attttttcag
     2641 ccggtgaagc aaggatcatt tcaggtcgtc cgccttccac cgaaacttta tataattcag
     2701 acaacatgga tgtaggaaac aaggcactct tcaccgggtc ctgataatga gcggaatata
     2761 caatgtattt cccgtcaggg gtaaatgtgt agggtttctc ggtagaagta tgcgtggtca
     2821 ggcgtttggc cgttccaccc gaagccgaca tgatgaatat atccctgttc gagttttcac
     2881 gatcactgac aaatgcgatt ttctgtccgt cgggcgacca gatcggattg ctgtcgaatg
     2941 cattgttcgt agtcagtctt gcagcctgac caccttccac ggcgactttg taaatatccc
     3001 cctgataaga aaatgcaatt tgtgaaccgt cgggtgaaat ggcgggatac ctcatccaca
     3061 aggggttttc ggcattgata aataaacagg aaagtaaaaa cagacctgat aaaacgattt
     3121 ttttgttcat gtgtaattgt ttttggtttt tttgaagcaa caaatgtatg aataatttcc
     3181 ctatgggaaa aatataaaat tttacaataa aaaaaagaaa cttctgttct cttttgataa
     3241 aaaacagcta ttttcgtaat ccgaattttt taattttata aattctgaaa ccatgaaaaa
     3301 actgttattt ttcttattcc tgagtttctt ttctttttca tctgctcagg tcagaaatga
     3361 aatcaacatc cctgatatta tgggatataa aaccctgaaa tgtgattttc acatgcacac
     3421 tgtgttttcc gatggactgg tgtggcctac cgtaagggtg agtgaagcct acaccgaagg
     3481 gctcgacgcc atagctatta ctgaccacat tgagtacagg ccccataaaa gtgatatgcc
     3541 tgccgcatcg cataaccgct cgtatgaatt agccgaagca tcggccatag cgagtggaat
     3601 tttgctgata cgcggctcgg aaattacacg cgccatggct ccggggcatt ccaatgcgct
     3661 gtttctcagt gattgcaacg cactggatgt gcctgcctgg caggatgcat tcaaagcagc
     3721 caaagcccaa aatgctttta ttttctggaa ccatcccggc tgggatgccc agcaacccga
     3781 tacaacccgc tggtttaagg aacataccga actttacaac gccggtttga tgcagggaat
     3841 tgaagtggtc aacggtccgg aatatttccc cgaagcacac cgctggtgtc tggagaaaaa
     3901 actgaccatg cttggcaata ccgatgtgca tgaaccgctg gcattcaaag acgggaaaca
     3961 ccgaaccatg acactggttt ttgcaaccga acggacacag gaggctataa aagatgcgtt
     4021 gataaacaga agaacggtgg tatatctgga cgacaaacta attggtgaag agagatacct
     4081 gaatgcgctg tttcatggat caatcgaaat tcttaaaacc gaaaaaacat caacccaata
     4141 tcgcataaca gtgaaaaata actccgatat tccgttttat cttcataaaa catcccataa
     4201 tccggatttg gtttatttcc gcgactacac gctggagcct cacagccaac acacaattac
     4261 tgtaaaacta acgaaaggaa ttaccggcgg tgatgtcaat tttgaaataa ccaatctgct
     4321 ggtcgaaccc ggtaagggac ttcaaataag attccccatt taacaaataa ccatcgaaac
     4381 ttgaaaattc ccatttcaat acctttttat gaaaatttaa acaaattatc ttttcaaacg
     4441 gcatttattt ttcaactaaa acgctatatt tgagcattat tattttcaat ttcataactt
     4501 tcttctaaaa aatacaaaga tggatttatc tgaattatta aacagttcaa ttggacaatc
     4561 aattgtgaaa agtgttgctg gtcagtttgg gctcgacgaa agtcaggctg catcggcagt
     4621 gaatacagca gtacccgtga ttttgggcgg attgaataaa aatgctcaaa ctcccgaagg
     4681 agccgaaagc ctcaacagcg cactggaatc caaacacgac ggaagtttac tggataacct
     4741 cggaggactt ctttcgggta atgtaggcga tctcttgcag gatggcggtg gaattttagg
     4801 ccatgttttc ggagataaag taggtgccgt agaaaatggt gtagctcaaa aaaccggcat
     4861 cagcaccgat aagattggtc ctattatttc catgcttgct cccattgtaa tggcatatct
     4921 gggcaaacag aaaagtcaaa gcaacgtaag tgcgggcggt cttggtgatc tactgggtgg
     4981 cttgctcggt ggcggtggct ctgctcaaag cagcggtggt ggcggactga tggatatggt
     5041 aaccggtttg ctggataaag acaaagacgg aagtgccctg gacgatattt tgggcatgtt
     5101 tactaaaaga taattcctta taaaacgata aaaaaaagag ctttccggat tgggcagctc
     5161 tttttttatt gatactttta ttgaatttat cgatatctga tataacaaat cagataaatg
     5221 caagaataat taattgtgcc attataactc tcagaaacat ggtgagagga taaaccgtgg
     5281 agtaagccac agcaggttga tcgttgcttg aaatggaact tgaatatgcc aaagcgggtg
     5341 ggtcggtagt gcttccggcc agcaaaccaa taagcgtgaa gtagttgagt ttggcaaatt
     5401 tccttccaat aaaaccgata ataagtaaag gtaatacggt aatagcgaaa ccgataccta
     5461 cccatagcaa accctgaccg gtgaacaccg tttctacaaa cttgccacct gcagccagtc
     5521 ccacactcgc caggaagagt gaaattccca cttcacgaag catcagattg gcactttggg
     5581 tagtataggt aatcaacttc aacttatatc cgaaccgacc gataagtatg gcaataatga
     5641 gcggaccgcc agccagaccg agttttacgg gcataggcat tcccggaatg aaaaatggaa
     5701 tactgccaaa caggataccc agaaaaattc caacaaaaat agtcacaatg ggaggctcgt
     5761 tcaatctttt caacgtgtta ccaagtagtt tttccacttt attgatgttt tccagttcgc
     5821 ctaccaccat cacacggtca cccacctgca aggtaagatt ggtggcaccc agcaggtcga
     5881 tacccgaacg attgacgcgg gtaatattaa ctccgaaaag tgtacgcagg cgcagtgatc
     5941 ccagagtttt tccattaatt ttactgtggg taatcactat tcgacgcgaa accaggtgtt
     6001 tctcggaatc tttccagtcg agttcggcgg gttcgccgat gaaagcagtg atggtctctg
     6061 tatcagccag tgctgatatt acaaggagta tatcgccatt tttaagtatt gtttccgatt
     6121 gaggtatgga atagatgcca ttgctcatca tgcgggagat gataaaattt cgtcccacca
     6181 tcgtttttac ctgatggagt gtttttccac tgatggcgtt attggtcagt ttaagggtta
     6241 ttcgttcggg tttttccata gccgtatctt cgtcctgctc cagcgctttg gcttccttct
     6301 ccagattgat gcggaatatc aaccggataa gaatgataga actgatgatg cccaccacac
     6361 ccagcggata ggccacagca taacccagtg caatttgagg gatttcggca atctgtccgg
     6421 cttcatgagc ctgacgcagc gcttcctgtg cagcacccag tccgggagta ttggtaatgg
     6481 cacccgacat gatacctact atcatgggca tttctacgcg cccattgagg acaaaataaa
     6541 aggaaactgc cactgtcaca cccagcaaga ctattccggc agccaacaga tttaagacca
     6601 ttccaccttt tttgaacgaa gagaaaaaac cgggaccaac ctgtaaacca atggaataga
     6661 taaacagaat taaaccgaaa tctttcagaa aatcaagcac ttcgtggttg atggtgtaac
     6721 ccatgtgacc cactaaaata cccataaata aaatgaaagt ggtgcccagc gaaatgcccc
     6781 agaattttat tttgcccaat agtactcccg taaagattac aaaggagtaa attaatatgg
     6841 tgtgagcaat cccttcaccg aataataaag aatttaacca ttccatagag tttgtaatta
     6901 gttgatatat agttaaataa caaaaattga agttcctgta tttgtgggtg caaaattagt
     6961 tgtttttatt caaaaattca gtatatcgaa ctaataaaac tgcttaaaat aataaattgc
     7021 ttaatatcag taatttgtaa atttttatgg gaggtaaagg tgaacgtttt ttgtggtgaa
     7081 agattttaaa atatatcagg tccaatgaaa cgggattgct accttaaaat catatttcaa
     7141 accgagtact gcgaaaattt attcataaaa cgctcataat aagtcttcga aaccggtatg
     7201 tcgggtgtgt tctctgcatc cagttctatg aaaatgccgg tctttgtttt gcgcagcact
     7261 ttgactttgt ccagattgac aatgtaggat cggtggcttc gaaccagtaa ggtgtctttt
     7321 atgagatttt catccatcca cttcagggtg ttacgcagca tgtaatgcga cagtttggat
     7381 ttgttcaggt agtgtaaggt gatataattg tcggccgaat caatgtaaag aatattctcc
     7441 tgcatgatag atattttcag ttctcccttt tcgtcgggaa atgctatcat cgatttggag
     7501 tgattttcaa ggacggtgtc ctccttttca atgttttcga gcatattgtt tttttctttc
     7561 catgaaaaat acagccagga aatggagtag ggcagcagca gtacaagccc ggtattaatg
     7621 gtgcttttac ggaatatttc cataaaatcg cggttcatcc cctcacgtgg aataaacttg
     7681 gtgaacaacg cataaaaaag tgacatggca agcacttcgc acaatatcca gccggcaaac
     7741 tgccagtagt tcaggttatg tcttcgggtg taatgcagca tgatcattcg gcttattaca
     7801 accaccagca ttccggtaag tataattaaa cttgaaaata cgaaatactt gaagtcagaa
     7861 atctgtggat accaaccccg tgagccgaat ggctggaaaa aattgataaa aagcagggca
     7921 aatatggccg tgaatataat taaacgaata atattgccct tttggtaaag atatgacggt
     7981 attttggtat tcatggcgtt cgggttacga cggttaaacg atacaaaagt aagccaaaat
     8041 atgaaatctg ctaatgttga aatgtttttt tcaccccgct atttgaaatt tcacccctca
     8101 ttcag
//
LOCUS       DAOPZK010000085         8092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_10644_length_8092_cov_21.043673, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000085 DAOPZK010000000
VERSION     DAOPZK010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10644_length_8092_cov_21.043673"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PKH68_06290"
     CDS             <1..1185
                     /locus_tag="PKH68_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12582.1"
                     /translation="LELNKYYQKWSDISNNDRLYPFTGYEITQDAPKILSFSGKLLTG
                     DRTLTLSKTEIGGTKNYGSGYNILSNSYTAAIDIAKIVFPATGVDKTVYLYNTGSLED
                     WGTQYKISGNVAGAYQAIPYNTSSVLGPIPSMQGFLLIATTPGAAVTIPYNSTVSAAT
                     ANTTPQRAPVEKIAANSLSWLTVDVTGKTGGDRVWIFSQDGTTHGFDNGWDGQKINQS
                     DGISLYVDEESGKYQVSTADNLNETWLAFQAGEETEYTLNINTTALKGYKSIFLKDTV
                     ANKTIKLSDSITLYPFTAANKGKFVRRFLLKAKTENNVKENIDPLLVRSFAAGNMLKL
                     QNRTAEIMTYMVHDIFGVQLVNGTVDPYSMTSPQMKFSSGIYFVVIKTTDFNKTEKVM
                     VK"
     gene            1311..2522
                     /locus_tag="PKH68_06295"
     CDS             1311..2522
                     /locus_tag="PKH68_06295"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNX12583.1"
                     /translation="MKTARFLIILLFVSCYAPGQNKAFVQVTDTAGNHAIPVLAWYSI
                     PEPFTTAERFGELAETGINLHFSYYSSADAAQKALDLAEKYHLKVIVSCPELETETEN
                     TVQRFKNHPALAGYFLHDEPITTQFKKISDWVKRIQAIDSTHFCYINLFPNDVVPRKL
                     KASGYRNYINRFLREVPVRFLSFDHYPVMGETLRPIWYENLEIIADASRKFKLPFWAF
                     ALTTAHDYYPVPDMAELRLQVYSNLAYGAQGIQYFTYWTPPVGDYDFHDGPITPDGLR
                     TPAYELIKAMNAEIRGYSDVFLGARVLTVRHTGTTIPRGTKRLTTLPAPFTKLVTGET
                     GAVISVMQKGDKKYLMMVNRNFLSTLELRIEFKSESVKQVLKNGETKLLPSGLSSFLL
                     DPGDAIVLNYE"
     gene            3006..4247
                     /locus_tag="PKH68_06300"
     CDS             3006..4247
                     /locus_tag="PKH68_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12584.1"
                     /translation="MPKGIKVDDQLIIEKFKKKVANQLPFSLKINKNYSRFSDLILQK
                     TETEVSVSTLKRLFLYESDVTPSRNTLDLICQTIGIKDWDAFLNSSKDYTEFEHLEVI
                     KSLELEGYRNKSDFKKIYEKFDYPEYKYFVTLYLIKNAIQKRDIDILKEIFILPVFHR
                     PDPGNVHQMYFLEYFGILFRNDDIIYELIPYWAKDPVGQTAYCEFFVDVDNLKGYYGA
                     VMEEYHKYKRNPEGLLFYHCMMYERDLQNGIIESKNIQFLLNFDEKAPVKSIHKTRRL
                     ALIITYNRLKGYPVDTYLDQIPDLVSLNDEYLRVLNVYTFCFLVFDKNDFYPLKEMLS
                     HFDQPNKRDDNNIFTFRLLNMIKLYQSFVHFREWETEKAIDLFQSIDQMYFSLYSIEQ
                     YKKQYKLVEKMIQAEKKKINL"
     gene            complement(4533..>5234)
                     /locus_tag="PKH68_06305"
     CDS             complement(4533..>5234)
                     /locus_tag="PKH68_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNX12585.1"
                     /translation="SPSVEKCVSGFFKMASGLSTDRIKFDYQAYIRDNEKILNYFSEN
                     RNLLSHPVNPLRIKAIELFGESKLYKAISQGEDLKHDEELSKNMEQLIDILITISSSP
                     MDAQRKLFVASAGILMAQVDKEMDEEEYEQILEALSMYTVFPKEFLSEMCKEDVEKIF
                     KESVKKIISQNPAERTQLLEYMASVAFCDSNMFPSEMDFLFETGEKILGLTRRETAQI
                     MALTVQQGFIPELYK"
     assembly_gap    5235..5334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5789..7198
                     /locus_tag="PKH68_06310"
     CDS             5789..7198
                     /locus_tag="PKH68_06310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX12586.1"
                     /translation="MKVTFIGAAREVTGSKTLITTIEGKKILLDCGMFQGKGLETDEL
                     NRRTGVNPAELDHIILSHAHIDHSGLIPYFYKLGFRGSVICTTATRNLCSIMLPDSGH
                     IQEADTEWFNKKRARQNLPPVNPIYEAKDAEEALKLFIAVDYNRRFYINDKVNVKFTN
                     TGHMLGGGVVNLEIIENGKKIHLAYTGDIGRPHNRIIKGPEAFPQCDYLITESTYGNR
                     LHDTEKQTEEDFLKIITDTCVVNKGKLIIPSFAIGRTQEVVFVLNKFFNDGLLPRVKV
                     YVDSPLAVNATDIFRMHLDLMNEDVKREIKEGDHDPFSFNNLIYIKKVEQSKELNDLK
                     EPCIIISASGMAEAGRIKHHIANNISNPHNTIMMVGYCSPTSLGARIQEPGLKEISIF
                     GEMHVVKARITRMEAFSGHGDYLEMKSFLDCQDKSQVKKTFLVHGEYEVQQFYKGILT
                     EDGFENIEIPAYLNEFELS"
     gene            7338..>8092
                     /locus_tag="PKH68_06315"
     CDS             7338..>8092
                     /locus_tag="PKH68_06315"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNX12587.1"
                     /translation="MLKKLIFPILLCLSTTGFSQNTYKMLVGTYTRGTTSEGIYSLEI
                     NKQGQLIFNKLLAKSDNPSYLAFSPYGKYVYAVNELGEESTVSAFAFDKENETLTFLN
                     KVSAGGADPCFITVSDRHVFTANYSSGSISVFERHPDGTISEAVQVITHPKKNFGNRR
                     FGPSNAHQVIFSPDGKYLMATNLGTDRVFTYSYNPIGSKEVLTYVDETGVKKHSGPRH
                     TVFSKNGKYLYLVQELDAGITVFSVANDGKLTN"
BASE COUNT         2485 a         1689 c         1549 g         2269 t
ORIGIN      
        1 ctggaactca acaagtatta ccaaaaatgg agcgacataa gtaacaatga tcggctgtat
       61 cctttcaccg gttacgaaat cactcaggat gctccaaaaa ttctttcatt cagtggaaaa
      121 cttttaaccg gcgaccgcac actgacatta tccaaaacag aaatcggcgg aacaaagaat
      181 tacggctcag ggtacaatat cctgagtaat tcatacactg cagccatcga tatcgctaaa
      241 atagtatttc ccgcaaccgg agtggataaa accgtatacc tgtataatac cggtagtctg
      301 gaggactggg gaactcagta taaaatatcc ggcaatgtag ccggtgcata tcaggctata
      361 ccctacaata caagttctgt gctgggacca attccctcca tgcagggatt tctgcttatt
      421 gccactaccc caggcgctgc tgtcaccatc ccttacaaca gcacggtctc ggcagccact
      481 gcaaatacta ctccacaacg ggctccagtg gaaaaaatag ctgccaattc attatcctgg
      541 cttaccgtag atgtaacggg aaaaaccggt ggcgaccgtg tgtggatatt cagccaggac
      601 ggaactactc acggatttga caacggttgg gatggtcaaa aaatcaatca atcggacgga
      661 atatcgttgt atgtagatga agaaagtgga aaataccagg tcagtacagc cgataatctg
      721 aatgaaacgt ggctggcatt ccaggccggt gaagaaaccg aatataccct gaatataaat
      781 actacggcat tgaaaggata caaatcgatt tttctgaaag atacagtggc caacaaaacc
      841 atcaaactga gcgacagcat cactctctat ccgttcacag cagctaataa aggaaaattc
      901 gtcagacgat ttttattgaa agcgaaaact gaaaacaatg tcaaagaaaa cattgatccc
      961 ttacttgtca gatcctttgc agccggtaat atgctgaaat tacaaaaccg cacagctgaa
     1021 attatgactt atatggtgca cgatattttt ggtgtacaat tggttaacgg aaccgttgat
     1081 ccatattcaa tgacctcccc gcaaatgaaa ttcagttccg gtatttattt tgttgtaatt
     1141 aaaacaaccg attttaataa aactgaaaaa gtgatggtta aataaagaaa cttaactaag
     1201 ccttaaagag caacttttag gaggttgctc tttaagtttt aaaccagctt tactttatat
     1261 tttaatcgtt ttttttatgt aatattgtac cagttattca actaaacacg atgaagaccg
     1321 cacgcttttt aataattctt ttgtttgtaa gctgttatgc tcccggacag aataaggctt
     1381 ttgtacaagt cacagataca gctggtaatc atgcgattcc ggtattggcc tggtacagca
     1441 tacccgaacc atttactact gccgaacggt ttggtgaact ggccgaaacg ggcatcaatc
     1501 tccatttctc atactattcc agtgccgatg cggcacaaaa agcactggat ctggccgaaa
     1561 aataccattt gaaagtcatt gtatcatgtc ccgaactgga aaccgaaacc gaaaacacag
     1621 tgcaacgatt taagaaccat ccggcactgg caggatattt tctacacgat gagccaatta
     1681 ctacccaatt taaaaaaatc agtgattggg taaaacgaat acaggcgatt gacagtaccc
     1741 atttctgtta catcaatctc tttcccaacg atgtagtgcc ccgtaaactg aaagcatcgg
     1801 ggtataggaa ttatatcaac cggtttcttc gtgaagtccc cgtccgcttt ctgtctttcg
     1861 atcattatcc agtcatgggt gaaacattac gaccgatttg gtatgaaaat cttgaaatca
     1921 ttgcagatgc ctcccgaaaa ttcaagctgc ctttctgggc atttgcactc accacggctc
     1981 acgattatta tccggtgccc gatatggcag aactgcgact tcaggtttac agcaacctgg
     2041 cttatggcgc tcagggtatt cagtatttta cctattggac tcctccggtc ggcgattatg
     2101 attttcatga tgggcccatc acccccgacg gcctacgaac cccggcttac gaacttatca
     2161 aagccatgaa tgcagaaata agaggatact cggatgtgtt tctgggcgcc agggtactta
     2221 cggttcgaca caccggcaca actattcccc ggggaacaaa acgactgacc accctgcccg
     2281 ctcccttcac taaactggtg acaggtgaaa caggtgctgt aatttcagta atgcaaaagg
     2341 gtgataaaaa atatttgatg atggtcaacc gcaattttct gagtacgctg gagttacgga
     2401 tagaatttaa gtcagaatcg gtgaagcagg tattaaaaaa tggtgaaacc aagcttttac
     2461 cttcgggatt atccagcttt ttgttagatc ccggcgatgc catagtatta aattatgaat
     2521 aatattttcc catattcctt caaaatcaga ttatttatgt tactgttgtg atattttaac
     2581 ttattgatga tacatatttt cttcatgttg agctttctcc acataccgga cctcaaactt
     2641 ccaaaatcaa attcaaatca ggccgtttat tatttaacac tctgctaatg tttacaataa
     2701 ttcaaaattt agaataaaaa tcgtcaatta cggactttca tttcaccaaa agatttttac
     2761 ctattcagat acatttcttc tctgttaaga ataattaact cataattttt ctatttgaag
     2821 tcatatgaac taatatgaac acgatatttt aatttaattt aattttattt gtataaataa
     2881 tttttccaat aagaaaaggc cttatttctc agtttttttt tcgcattgaa attgttttgt
     2941 aataacaaca ttttaatgta atagttcata attctctgtt tgtttaacat tttaaatttg
     3001 ttaccatgcc aaaagggata aaagtggatg atcaattaat catcgaaaaa ttcaaaaaaa
     3061 aagtagctaa ccaattgcct ttttcgctta aaatcaataa gaattacagc cgcttctccg
     3121 atctgatatt gcaaaaaacg gagacagaag tgagtgtaag cactctgaaa aggcttttct
     3181 tgtatgagag cgatgtgact ccttcgcgca atacgctgga cttgatatgt cagaccatag
     3241 gcataaaaga ttgggatgca ttcctgaatt cctcaaaaga ttacacggaa ttcgaacacc
     3301 tcgaagtgat taaaagtctg gagttagaag gttatcgcaa taaaagtgac tttaagaaaa
     3361 tctacgagaa attcgattat cccgaataca aatacttcgt tacactctat ttgattaaaa
     3421 atgccattca gaaaagagac attgatattc tgaaagaaat tttcatactt cccgtttttc
     3481 atcggcccga cccaggaaac gtacatcaga tgtacttttt ggaatatttt ggcattctat
     3541 ttcgcaacga cgatattatc tatgaactta tcccctactg ggcgaaagat cctgtagggc
     3601 agactgctta ctgcgagttt ttcgtagatg tagataatct gaaaggatat tatggcgctg
     3661 tgatggaaga ataccataaa tacaaacgca atccggaagg tctgcttttt tatcactgca
     3721 tgatgtacga acgcgattta caaaatggca tcatcgagtc gaagaacata cagtttctgc
     3781 ttaattttga cgaaaaagca cccgttaaat cgattcataa aaccagaaga ctggcattaa
     3841 ttattacata caatcggttg aaaggctatc ctgtggacac ctacctggat caaatccccg
     3901 acctggtatc gctgaatgac gaatacctgc gtgtattaaa tgtttatacg ttttgctttc
     3961 tggtgtttga taaaaacgat ttctaccccc tgaaagaaat gttgtcgcac tttgaccaac
     4021 caaataagag ggatgacaat aatatcttta cattcagact gttgaatatg attaagttat
     4081 accagtcatt tgttcatttc agagaatggg aaacagaaaa agcgattgat ctttttcagt
     4141 ccatagatca gatgtatttt tcgctttaca gcatcgaaca gtacaagaaa caatacaaac
     4201 tggttgaaaa gatgatacaa gctgaaaaga agaaaattaa cttataagca gttgcctgca
     4261 acaaatcgtc ccggttaaag tcggggcgat ttagaatcac tgttgtccgc taaaattcta
     4321 aattcttgaa tatattagat ttctcactcc gttcgaaatg actgtcagta ttttatcttt
     4381 tgggaggggg ttgtttggca gcttcgctgc caaacaaccc cctccctcca taaatctcca
     4441 taaatccctg tcatcctgag cgcagcgaag gaactaaata tttctaccag acaattgtga
     4501 tttctaatat aagttgttaa aattatccat tcttatttgt acaattcggg aataaaccct
     4561 tgctgcactg tcagggccat gatttgtgcc gtttcgcgtc gggtaagtcc cagaatcttt
     4621 tctcccgttt cgaaaagaaa atccatttca ctgggaaaca tattgctgtc acagaaagct
     4681 acactggcca tatattcgag tagttgggtt cgttcggccg gattctgact gataattttt
     4741 ttcactgatt ccttaaaaat tttctcaaca tcctctttgc acatttcact caaaaattct
     4801 ttgggaaata cggtatacat gctcagtgcc tccagaattt gctcgtactc ctcttcgtcc
     4861 atttctttgt ccacctgggc catcagtatt ccggccgatg ccacaaatag tttccgctgt
     4921 gcatccatgg gcgacgaact gatggtaatg agaatatcta tcagttgttc catgtttttg
     4981 gaaagctctt catcgtgttt taagtcctct ccctgtgaaa tagctttata cagtttcgac
     5041 tcgccgaaaa gttcaatggc cttaatccgg agcggattta ccggatggct cagcaggttt
     5101 ctgttttcac tgaaataatt cagaattttc tcattatccc tgatatacgc ctgataatca
     5161 aactttatgc ggtcggtact caggcccgat gccattttga aaaaaccgga aacacatttt
     5221 tctacactcg ggctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctatc
     5341 attttcccct ccagcatctg ataaattttt tcttccagat caatctcgag gcttacccgg
     5401 acgtgatttg tgttcatgta atttgtgttt tgttggttta aaataagccc cccaaccccc
     5461 taaaggggga gttgaagtgc gttaactgaa tatgtttacg aaataggtat tcggaactaa
     5521 ttattaaatt taaaaatcaa actgcagaac aaaatacagt ttttcattaa agctgataaa
     5581 atcaattctt tggttttgat ttttttacct gtcttgatac aaactgcttt gatttctact
     5641 ttgttacgga ttataaagat agaattaaaa tttcttaagt aaaatattta cgtaggaaaa
     5701 ataagataat taagcatcgg gttgttaatt accgattaaa aactctattt ttgtagtagt
     5761 cgaaattaat taacaaaata ccaaaaatat gaaagtaaca tttattggcg ccgcccgtga
     5821 agtaaccggt agtaaaacac tgataaccac tatcgaagga aagaaaatac tgctcgattg
     5881 cggcatgttt cagggaaaag gtcttgaaac cgacgaactg aaccgccgta ccggagtgaa
     5941 cccggctgag ttggatcata tcattctctc acacgctcat atcgatcact cgggacttat
     6001 cccctacttc tacaagcttg gttttcgtgg ttcggtgatt tgcaccactg ccacccgtaa
     6061 cctctgctcc atcatgctcc ccgacagcgg acatattcag gaagctgata ccgaatggtt
     6121 taacaagaaa cgggctagac aaaatctgcc tcccgtaaat ccaatctatg aagctaaaga
     6181 tgctgaagag gcactgaaac tatttattgc tgtggattac aatcgccgct tttacatcaa
     6241 cgacaaagtg aatgtgaaat ttaccaacac cggtcacatg ctgggaggtg gagttgtgaa
     6301 cctcgaaata atcgaaaatg gcaagaaaat tcatctggct tacaccggtg acattggacg
     6361 gccgcacaac cggataatta aaggtccgga agctttcccg cagtgcgatt acctcatcac
     6421 cgagtccacg tatggcaacc gccttcacga taccgaaaaa caaacagaag aggattttct
     6481 gaaaataatt accgacacct gcgttgttaa taaagggaaa ctaatcattc catcctttgc
     6541 catcggacgc acgcaggaag tggttttcgt gctcaacaag ttcttcaacg acggattgct
     6601 gcccagggtg aaagtgtacg tggacagtcc gctggccgtc aatgccaccg acatattccg
     6661 tatgcacctg gatctgatga acgaagatgt aaaacgcgaa ataaaggaag gtgaccacga
     6721 tccgttcagc ttcaacaacc tgatttatat caaaaaagtg gaacaatcaa aagaactgaa
     6781 cgacctgaaa gagccctgta tcatcatttc ggcatcggga atggccgaag ccggacgtat
     6841 taaacatcac atagccaaca acatatccaa tccgcacaat acaatcatga tggtgggcta
     6901 ctgctcacct acttcactcg gcgcccgcat tcaggaaccg gggctgaaag aaatatccat
     6961 ttttggagaa atgcacgtag tgaaagcccg cattacccga atggaagcat tctccggtca
     7021 cggtgactac ctcgaaatga aaagttttct cgattgtcag gacaaatcac aggtgaaaaa
     7081 aacattcctt gttcacggtg aatacgaagt tcagcaattc tacaaaggaa ttttgacgga
     7141 agacggattt gaaaatatcg aaataccggc ttacctaaat gaatttgaat tgagttagga
     7201 aaagtagccc cctaaatccc ccaaaggggg acttatgtgc aattagttga attttagaat
     7261 tttattaatt gtacataatc tcaaatacta actgcaaact gcaaactgct aactgcaagc
     7321 tgctaactaa aacatttatg ctaaaaaaac tgatatttcc aattctgctc tgcctgagta
     7381 ccaccggttt ttcgcagaat acctataaaa tgcttgtggg aacttacacg cgcggaacta
     7441 cgagtgaggg aatttactca ctcgaaatca acaagcaggg tcagttaatc ttcaacaaat
     7501 tacttgcaaa aagtgataat ccaagttacc tggccttttc gccctatggg aaatacgtgt
     7561 atgctgtgaa tgaacttggc gaagaaagta ccgtcagcgc atttgctttt gataaagaaa
     7621 atgaaacact gacttttctc aataaagtaa gtgccggagg ggcagatccg tgttttatca
     7681 ccgtttctga ccgtcatgtt ttcacggcca attattccag tggaagtatt tccgtatttg
     7741 aacgacaccc ggatggcaca ataagcgaag ccgtgcaggt aatcacgcat ccgaagaaaa
     7801 atttcggcaa tcgtcgtttc ggcccgagta atgctcatca ggtgattttt tcgcccgatg
     7861 ggaaatacct gatggctacc aatctcggca ccgacagagt ttttacttac agctacaacc
     7921 ccatcggaag taaggaagtg ctgacttatg tggacgaaac cggggtaaaa aaacacagtg
     7981 ggccgcgtca cactgtattc agtaagaatg gcaagtatct gtatctggtg caggaactgg
     8041 atgcgggcat caccgtattt tcggttgcca atgacggaaa actgactaat at
//
LOCUS       DAOPZK010000086         8054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_10713_length_8054_cov_20.660333, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000086 DAOPZK010000000
VERSION     DAOPZK010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10713_length_8054_cov_20.660333"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKH68_06320"
     CDS             <1..315
                     /locus_tag="PKH68_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNX12588.1"
                     /translation="TLCHDFSNRKLFKVEILTDKDRGEALQKQFLKDYQQHLGISEYE
                     AGYFLGVETVSTDTYSPEDDTIDILQKDGTVKDIAEASDMLNIQVLTKKVEKHFLCYY
                     RL"
     gene            455..1498
                     /gene="lpxD"
                     /locus_tag="PKH68_06325"
     CDS             455..1498
                     /gene="lpxD"
                     /locus_tag="PKH68_06325"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNX12589.1"
                     /translation="MEFSAQQVASFLNGEIIGDPTVSVHDFSKIEEGQTGTLSFLSNP
                     KYNQYIYDCNSSIVLVNRSFVPEREVKPTLIKVDNAYESLAVLMGIVEEHKPKKNGVS
                     PLAFISPTARIGANVYVAPYVFVGDHVEIGNYTTIDASAYIGDGVKVGNNCQLHAGVK
                     IEKECVVGNNCILHAGVVIGSDGFGFAPTDEGTYRKIPQTGNVILEDNVEVGANATID
                     RATLGSTIIRKGVKIDNLVQIAHNVEVGENTVIAAQSGVAGSTKLGKQCVLAGQVGIA
                     GHLEIADGSIFGAQTGVPGSLKTPNQIYQGYPAVPAGIFRRATVVFKSLPELQKTVFE
                     LQKQLDELKSKKK"
     gene            1583..2968
                     /locus_tag="PKH68_06330"
     CDS             1583..2968
                     /locus_tag="PKH68_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNX12590.1"
                     /translation="MATQKTLKTFFELNGKGLHTGLDINIKFSPAAPNTGIRIKRTDL
                     PNQPEISALADYVADTNRGTVLKRGEFQVSTIEHAMAALYASGIDNCHIEVDAPEFPI
                     LDGSSRYFIEGILQVGIEDQPKAEKDVFVVRKRMEYKHPETGAKIVLLPDDNFSVDVH
                     IGFNSPVLSNQFASIESMDEFASEISGARTFVFVREIEQLLHQNLIKGGDLENAIVIY
                     DRQMPQEEFDKLADMMNQPHLNVGELGIISGDLKFDNEPARHKLLDVIGDLALTGRQI
                     QGKVIATYPGHKVNTEMAKFIRKEIKKQEVQAPVYDDTIPPVLDTIAIRKMLPHRWPF
                     LMVDKIIEIRERSVVGVKNITVNETLFMGHFPDEPVMPGVLLVEAMAQTGGLLVLSQL
                     DDPERYSTYFMKIDNVKFRQKVVPGDTVIFHVSLVDDIRRGIATMRGYAFVGGVITTE
                     AEFMAQIVKNK"
     assembly_gap    3094..3193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3221..4000
                     /gene="lpxA"
                     /locus_tag="PKH68_06335"
     CDS             3221..4000
                     /gene="lpxA"
                     /locus_tag="PKH68_06335"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNX12591.1"
                     /translation="MKQPLAYIHPDAKIAPTVVIEPFVTIDKNVVIGEGTRIGSNVTI
                     LEGVRIGKNCTIFPGAVIGAVPQDLKFQGEDSLAIIGDNTTLREFVTVNRGTASKGKT
                     IVGSNCLLMAYCHVAHDCVIGDNVIMSNACQLAGEVEIDDYAILGGGTMIHQFSRIGA
                     HTMLQGGSKVTKDVPPFITAGRDPLSYAGVNSIGLRRRGYTNEQIRDIQDVYRFIYQS
                     GMNTSHSVERVQAELPATKERDEILQFIKNSPRGIIKGYFD"
     gene            4130..5797
                     /locus_tag="PKH68_06340"
     CDS             4130..5797
                     /locus_tag="PKH68_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNX12592.1"
                     /translation="MEKSLNFIEQIVEEDLAAGKNNGRIQTRFPPEPNGYLHIGHAKA
                     ICMDFGIAQDYNGICNLRFDDTNPVKEDVEYVDSIMEDIKWLGYEWENVYYASDYFQQ
                     LWDLAVKLIRDGKAYIDEQSAETIAKQKGTPTEPGTESPYRNRPVEENLALFEKMNTG
                     EIPEGAMVLRAKIDMASPNMHFRDPIMYRVISSHPHHRTGYTWKAYPMYDYAHGQSDY
                     FEGVTHSICTLEFVVHRPLYDWFIDQFKDSDYRPRQIEFNRLNITYTVMSKRKMLQLV
                     QEGLVSGWDDPRMPTLCGLRRRGFTPQSIHNFIDKIGYTKVEGMIDVGLLEHSARETL
                     NKSANRVSAVLDPVKLIITNYPEGQVEMMEAINNPEDETSGSHEIAFSRELWMEREDF
                     MEDAPKKFFRMTPGQEVRLKNAYIVKCTGCQKDENGNIQEIYCEYDANTKSGMPEANR
                     KVKGTLHWVSVAHCLPAEVRLYDRLFSVEDPSADDRDFRELLNPESLKVLTGCYVEEY
                     LRDARPLDHFQFQRIGYFNVDTDSAEGKLVFNRTVSLKDSWAKEQNK"
     gene            5873..6238
                     /locus_tag="PKH68_06345"
     CDS             5873..6238
                     /locus_tag="PKH68_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNX12593.1"
                     /translation="MKIFLTIGLLLCSNIFMTLAWYGHLKLVQYPWFSKISLIGVILF
                     SWGIALFEYFFQVPANRIGFQGNGGPFSLVQLKVMQEVITLTVFMIFSYVAFHETIRL
                     NQLIGFLFLILAVYFIFKP"
     gene            complement(6302..7834)
                     /locus_tag="PKH68_06350"
     CDS             complement(6302..7834)
                     /locus_tag="PKH68_06350"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNX12594.1"
                     /translation="MKKTVFSYFVLTTILLFSVAGLRAQVSTMYYLKNNPYRHQLNPA
                     FQPASGFYIDLPLLPSLELSVGNNSLTFGDIIYPKMINGQMKTITFMDENGDKDLFYN
                     TLKSQTNIQSNLQLDIFSLGFRSRNSYFTFGVSQKFNMNAGLPKDMFKLMIYGTPDTL
                     NVNRYNMSSLNISSTAYTEYALGYSREINDRLSVGAKAKWLVGEANISAGFNKLNMNA
                     DREHWDASIDGTVNGAIPFAKFILNNAGKIDSISSSFPAPNTDKGFGGYMDMVKEFMD
                     VMRHPSGSGFAVDLGAAYKISDKLSVSASVLDLGFIHWKRNTVNIPVSGNFSFGGIDF
                     NAEDSTGNFSENLGTISDSITYKTTFNPYNTSLQTKILAGVEYYVVKDKISLGLLSKS
                     TFINKKLVEEITTSANFFPADWFNASVSYSLMNGRFSNLGLGINTRVGPSNWFLVSDY
                     IPLRYTPEFIPYKTRSVNLKMGFCLTFGNGKKEKKEKEDKKTNNIEKSPNIIPDINPV
                     EE"
     gene            complement(7855..>8054)
                     /locus_tag="PKH68_06355"
     CDS             complement(7855..>8054)
                     /locus_tag="PKH68_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12595.1"
                     /translation="ADVNGTAYFKFNENEMAAISKIKFVVLKVAIKGMDENSLISLRP
                     QDYIRLKADLYINGDISLSNN"
BASE COUNT         2324 a         1680 c         1797 g         2153 t
ORIGIN      
        1 acactctgtc acgatttctc caaccgaaaa ctcttcaaag tggaaattct gaccgataag
       61 gataggggag aagcactgca aaagcaattc ctgaaggatt atcagcagca tttaggtatc
      121 agcgaatacg aagccggata ttttttgggc gtggaaaccg tctcgaccga cacctacagc
      181 cccgaagatg ataccattga tatcctgcag aaagacggaa ccgtcaaaga cattgctgaa
      241 gcctcggata tgcttaatat tcaggttctc accaaaaagg tggaaaaaca ctttctgtgt
      301 tattacaggt tgtaaagcct gagaaatgga cgaacattgg tgaaccattt tatgcttctt
      361 atcactccat gagcacggca cgctgaaatt ggcaccccga aaaggtgaat aattggaatt
      421 tcttttgtat ttttgcagcc taaattttaa caacatggag ttttctgctc aacaagttgc
      481 atcttttctg aacggtgaaa ttattggtga tcctacggtt tcggtacacg atttttccaa
      541 aatcgaagaa ggtcaaaccg gaacactgag ttttttatcc aatcccaaat acaatcaata
      601 catctatgac tgcaattcaa gcatcgtgct tgtcaatcgc agttttgtgc ccgaaagaga
      661 agtgaaacct actttgatta aagtggataa tgcatacgaa tcgcttgccg tattgatggg
      721 tattgtagaa gaacacaaac caaagaaaaa cggagtttcg ccccttgcat ttatttctcc
      781 tacagcccgt atcggggcta acgtatatgt tgcaccttat gtatttgtag gtgatcatgt
      841 cgaaatcggc aattatacca ccatcgatgc ctccgcttat attggcgatg gcgtgaaagt
      901 gggcaacaac tgccagctgc atgccggagt gaagattgaa aaagaatgtg tcgtaggaaa
      961 taattgcata cttcatgccg gagtggtgat tggttccgac ggatttggtt ttgcgccaac
     1021 cgatgaaggc acttaccgca aaataccgca gacaggtaat gtaattctgg aagataatgt
     1081 ggaagtgggc gccaatgcta ccatcgaccg cgctacgctg ggaagtacaa ttattcgcaa
     1141 aggggttaaa atcgacaatc tggtacaaat agcccacaat gtagaggtgg gagaaaatac
     1201 ggtcattgca gcccagtcgg gtgtagccgg atcaaccaaa ctcggaaaac agtgcgtact
     1261 tgccggacag gtaggtatag ccggtcatct cgaaatagcc gacggcagta tttttggagc
     1321 tcagaccggg gtgccggggt cattgaaaac accgaaccag atatatcagg gctatccagc
     1381 ggtgccggcc ggaattttcc gacgagcaac agtggtgttt aagagtttgc ccgaactgca
     1441 gaaaacagtt ttcgaactcc agaagcaact ggatgaactg aaatcaaaaa agaaataatc
     1501 ctatcaaata aataccaccg gattgtgtcc agctttcgat caagtagaaa gtgagcttac
     1561 aaatcctctc atttattatc gcatggctac tcaaaaaact ctcaagacat ttttcgaact
     1621 caacggtaaa ggacttcata ccgggctgga tattaatatt aaattttcac ccgcagcgcc
     1681 caataccggt atccggatta aaagaaccga tctacccaat cagcccgaaa taagtgcact
     1741 ggcggattat gtggcagata ctaaccgcgg aacagtactg aaacggggcg aatttcaggt
     1801 aagtaccatc gaacatgcta tggctgctct ttatgcttca ggcattgata actgccatat
     1861 tgaagtggat gctcccgaat tcccgatatt ggatggtagt tcacgatatt tcatcgaggg
     1921 aattctgcaa gtgggtattg aagaccagcc caaggcagaa aaagatgttt ttgttgtacg
     1981 taaaagaatg gaatacaagc atcctgaaac cggtgcgaaa atcgttttgt tacccgatga
     2041 taatttcagt gtcgatgtgc atataggttt caattctccc gtactctcca atcagtttgc
     2101 atcaattgaa tcaatggatg aatttgccag cgaaatatcg ggagcaagaa cgtttgtctt
     2161 tgtaagggaa atagaacagt tgctgcacca aaatctgatt aaaggcggtg atctggaaaa
     2221 tgcaatagtg atttacgacc gtcaaatgcc tcaggaagag tttgataaac tggctgatat
     2281 gatgaaccag ccgcacctca acgtcggtga actgggtatc atcagcggtg atttgaaatt
     2341 cgacaatgaa cctgcccgtc acaaactgct ggacgtaatc ggtgacctgg cgctcaccgg
     2401 ccgacagata cagggaaagg ttattgccac ttatcccggc cataaagtca ataccgaaat
     2461 ggcgaaattt atccgcaagg aaatcaaaaa gcaggaagtt caggcacctg tgtacgacga
     2521 taccattcct ccggtactcg ataccattgc catccgcaaa atgcttccac accgctggcc
     2581 atttctaatg gtggataaaa ttattgaaat acgagaacgc tctgttgtag gagtgaaaaa
     2641 tattacggtt aatgaaaccc tctttatggg acatttcccc gatgagccgg tcatgccggg
     2701 cgtgctgctc gtggaagcaa tggctcaaac aggcggattg ctggtgctga gccagttgga
     2761 cgatcccgaa cggtattcga cctattttat gaaaattgat aatgtaaaat tccgtcagaa
     2821 agtggtgccg ggcgatacag tcattttcca tgtttcgctc gtggatgata ttcgccgtgg
     2881 cattgccacc atgcgcggat atgcctttgt aggtggcgtg attactaccg aagccgaatt
     2941 tatggcgcaa atagtgaaga ataaatagtc ggctccctaa cccccttaag ggggaatgtg
     3001 ttgggaggat gaatatttcc cttttaatca acatttatta aaacaatatt aaatattaaa
     3061 gtacagaaaa cgctcttaaa attccccctt tagnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnngggttag ggggcaagct tatattaatt atgaaacaac cattagcata
     3241 cattcatccg gatgcaaaaa ttgcacctac cgtagtgatc gaaccttttg taacaattga
     3301 taaaaatgtg gttatcggtg aaggtacccg tatcggttcc aatgtaacca tcctcgaagg
     3361 tgtacgtatc ggaaaaaact gtaccatttt ccccggagca gttattggtg ccgtgcctca
     3421 ggatctgaaa ttccagggtg aggattcgtt ggctattatc ggcgataata ccaccttgcg
     3481 tgaattcgta actgttaacc ggggtactgc ctctaaaggg aaaaccattg taggtagtaa
     3541 ttgtctgctg atggcttatt gccatgtagc acacgattgt gtcatcggag ataatgtgat
     3601 catgagcaat gcctgtcagc ttgccggtga agtggaaatt gacgattacg ccattctggg
     3661 tggtggaacc atgatccatc agttcagccg cattggagct cataccatgc tccagggcgg
     3721 atcaaaagtt actaaagatg taccgccgtt tatcacagcc ggacgcgatc cgctttcgta
     3781 tgccggtgtt aattcaatcg gactgcgtcg aagggggtat accaacgagc aaatcaggga
     3841 tattcaggat gtgtatcgtt tcatttatca gtcgggaatg aatacttctc attcggtaga
     3901 gcgcgtgcag gcagaacttc ctgccacaaa ggaacgcgat gaaattctgc aatttatcaa
     3961 aaattcacca cgcggtatta ttaaggggta ttttgattaa aaaacccacc caaaccctcc
     4021 cgaagggagg gatgttagat gtaataaaag aagtacaagt actaaaataa tcaatcatca
     4081 gccaaaggct gtacacgtgt caaaagctga ccgaatattt attgggatta tggaaaaaag
     4141 tttaaatttt atcgaacaaa ttgtcgaaga ggatcttgca gcaggtaaga ataatggccg
     4201 aattcaaacc cggttcccgc ctgagcccaa cggctacctt catatcggtc atgccaaggc
     4261 tatatgcatg gattttggta ttgcacagga ctataatggc atctgtaatc ttcggttcga
     4321 cgataccaat ccggtaaagg aagatgtgga atacgtggac tccatcatgg aggacatcaa
     4381 atggctgggt tatgagtggg aaaatgtgta ttacgcttcc gattattttc agcagttgtg
     4441 ggatttggct gtaaaactta tacgcgatgg gaaagcatat attgatgaac agtcagccga
     4501 aaccatagcc aagcaaaaag gaactcccac cgagccggga acagaaagtc cgtaccgcaa
     4561 ccgaccggta gaagaaaatc tggctttatt cgaaaaaatg aataccggtg aaatacccga
     4621 aggtgccatg gtgctgcgtg ctaaaataga tatggcatcc cctaacatgc attttcgcga
     4681 tcccatcatg taccgtgtta taagttcgca tccgcatcac cgcaccggtt atacctggaa
     4741 agcgtatccc atgtacgact atgctcacgg acagtccgat tatttcgaag gagtaactca
     4801 ttccatctgt acacttgagt ttgtggttca ccggccattg tacgactggt ttatcgatca
     4861 gtttaaggat tcggattatc gtccccgaca aattgaattc aaccgcctga atataaccta
     4921 taccgtgatg agcaagcgca aaatgctgca actggtacag gaagggctgg tgagcggatg
     4981 ggacgatcct cgtatgccta cactttgcgg attgcggcgg cgcggcttta cacctcaaag
     5041 cattcacaat tttattgaca aaataggata taccaaagtg gaagggatga ttgatgtcgg
     5101 actgctcgaa cactccgccc gcgagacact caataaatcg gccaaccgcg tgagcgccgt
     5161 actcgatccg gtaaaactga tcatcaccaa ctatcccgaa gggcaagtgg aaatgatgga
     5221 agccattaac aatcctgaag atgaaacatc cggaagtcat gaaatagctt tcagccgtga
     5281 gctgtggatg gaacgcgagg atttcatgga agatgctccc aagaaattct tccgcatgac
     5341 cccgggacag gaagtacgac tgaaaaatgc ctatatcgtt aaatgtaccg gctgccaaaa
     5401 ggacgaaaac ggaaatatac aggaaatata ctgcgaatac gacgccaata ccaaaagcgg
     5461 aatgccggaa gccaaccgca aagtaaaggg aacactccac tgggtatcgg tggcacactg
     5521 tctgccagcc gaagtaaggt tgtacgaccg cctcttttcg gtggaagatc catcggccga
     5581 cgatcgcgat tttcgtgaac tgctaaatcc ggaatcgtta aaagtactca cagggtgcta
     5641 cgtggaggaa tatctccggg atgccagacc gctcgatcat tttcagtttc agcgtatcgg
     5701 gtacttcaat gtggatacag atagcgctga gggcaaactg gtattcaacc gtaccgtttc
     5761 gttgaaggac agttgggcta aagaacaaaa caagtaataa atttacaata acattatgct
     5821 aaggctgtct ttaaccggac agccttattt ttgaatacat ttttttttga caatgaaaat
     5881 atttctgaca attggactgc tattatgctc caacatattt atgacgctgg cgtggtacgg
     5941 acatctgaaa ctggttcaat atccgtggtt cagcaagatt tcgctgatcg gggtaattct
     6001 ctttagttgg gggatcgcac tgttcgaata cttcttccag gttcccgcca accgcatcgg
     6061 ttttcagggc aacggcggac cattctcgct ggttcagctc aaggtgatgc aggaagtgat
     6121 cacactcacc gtgttcatga ttttttcata tgtggcattt catgaaacca tcagactaaa
     6181 tcaactgatt ggttttctgt ttctgatact tgctgtttat tttattttta aaccctgata
     6241 tacagttgat tacagaaatc aaaaaagccg gttcaactga ccgggctttt tgatttctac
     6301 tttattcctc caccggattt atatccggga taatattggg tgatttttca atattatttg
     6361 tctttttatc ctctttctcc tttttctcct ttttcccgtt gccgaaagtc agacaaaagc
     6421 ccattttaag gtttacgctc ctggttttat aaggaataaa ttccggtgta tatcgcaggg
     6481 gaatataatc actgaccaga aaccaatttg atggcccgac acgggtatta atccccagtc
     6541 caaggttgct gaaacgcccg ttcatcaacg aatagctcac cgaagcattg aaccagtcag
     6601 ccgggaaaaa gttggccgat gtggtaatct cttcaactaa ttttttattg ataaatgtac
     6661 ttttcgataa aagtccaagg ctgattttgt cctttaccac ataatattcc acacccgcca
     6721 gtattttggt ttgcagcgaa gtattgtaag gattaaaggt cgttttgtag gtgatactgt
     6781 cggatatcgt accgaggttt tcactgaagt taccggtgct gtcttctgca ttgaaatcaa
     6841 ttcctccaaa actaaaatta ccgctgacgg gaatgtttac cgtatttctt ttccagtgga
     6901 taaagcccaa atcaagtacc gaagccgaaa cggacagttt gtcagatatt ttgtaggctg
     6961 ctcccagatc aacagcaaat ccgcttcctg aagggtgacg cattacatcc ataaattctt
     7021 taaccatatc catataacct ccaaatcctt tatcagtatt gggagcggga aatgaagagg
     7081 agatggaatc aatttttccg gcattattca gaataaattt tgcaaacgga atggctccgt
     7141 tgactgttcc gtcgatggag gcatcccagt gttcccggtc ggcattcatg tttaacttat
     7201 tgaaaccggc cgaaatattg gcttcaccta ccagccattt tgcttttgca cccacactca
     7261 accgatcatt gatttctctg gagtaaccta atgcatattc ggtgtaggct gttgatgaaa
     7321 tattcaaaga actcatgttg taacggttta cattcagtgt atcgggtgtg ccgtagatca
     7381 tcagtttgaa catatctttt ggcaaaccgg cattcatatt gaatttctgc gaaactccga
     7441 aggtgaagta cgaatttctt gacctgaaac ctaacgagaa gatatccagc tgaagattgg
     7501 actgaatgtt agtttgcgat tttaacgtgt tataaaacag gtctttatcg ccgttctcat
     7561 ccatgaatgt gatggttttc atctgtccgt tgatcatttt aggatagatt atgtcgccga
     7621 atgtcaatga gttattaccc acactaagtt cgagggaagg aagtaatggt aaatcgatgt
     7681 aaaaacccga agcaggctga aatgccggat tcaactgatg cctgtaggga ttatttttca
     7741 ggtaatacat ggtgcttacc tgtgcccgga gacccgcaac tgaaaacaac aaaatagtag
     7801 ttaagacgaa gtagctgaaa actgttttct tcatattatt ttttaacagg ggctttaatt
     7861 attacttagt gaaatgtcgc cgttaatgta aagatcagct ttcaggcgaa tatagtcctg
     7921 tgggcgtaga gaaatcagtg aattttcatc cattcctttt atggcaacct ttagaaccac
     7981 aaattttatt tttgaaatgg cagccatttc attttcattg aatttaaagt atgcagttcc
     8041 atttacatcg gctg
//
LOCUS       DAOPZK010000087         7800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_11232_length_7800_cov_25.746159, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000087 DAOPZK010000000
VERSION     DAOPZK010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11232_length_7800_cov_25.746159"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKH68_06360"
     CDS             complement(<1..743)
                     /locus_tag="PKH68_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNX12596.1"
                     /translation="MTIYPNLILEALKHVRYPGTGKDIVSAEMVQDNMRIENNKVSFS
                     ILFDKPNDPFAKSVVKAAEQAIITYIGEDVDIKGNISIITKELPKPKPASILPDVKNI
                     IAVFSGKGGVGKSTVSSNLAIALASMGYKVGLLDADIHGPSIPKMFGVENERPFVEEI
                     DGRQLMQPIERYGVKLLSIGFFVDPANALVWRGSMASNALKQLITDTVWGELDFFIMD
                     LPPGTGDIHLTLVQTMGITGAIAVTTPQDV"
     gene            complement(806..1555)
                     /gene="trmB"
                     /locus_tag="PKH68_06365"
     CDS             complement(806..1555)
                     /gene="trmB"
                     /locus_tag="PKH68_06365"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNX12597.1"
                     /translation="MGKNKLAKFAELETFTNVFQVKSGVLLGGEGFELKGKWNEQYFQ
                     NPNPIVLELGCGKGEYTVELARRFPDKNYIGVDIKGARIWSGAKDAFVNQLKNVAFIR
                     TNIEMIFHFFAPGEVSEIWLTFPDPQMKKTTKRLTATNFIRSYLQFLKPSGIVHLKTD
                     SNFMFTYTCEMVKLNDFEVLSHVDDIYAGENEKNPVLGIKTYYEQQWLDRGLTIKYIA
                     FRPAAGKVLIEPEINIEPDIYRSFKRFRRQQ"
     gene            complement(1558..2031)
                     /locus_tag="PKH68_06370"
     CDS             complement(1558..2031)
                     /locus_tag="PKH68_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNX12598.1"
                     /translation="MIKEISKYIVLIFIGVAVLSCNKKDIYFSYKTVSASGWNKDSLL
                     TYNVAIRDKTLPYNLYINVRHHGNYPYQNLWLFLENKDMKGMIQKDTIECFLADEFGK
                     WLGTGAGALKEMPVLYRQQVIFPDSGIYQMKIGQGMRDSILVGINDIGIRVEKAN"
     gene            complement(2117..3187)
                     /gene="ricT"
                     /locus_tag="PKH68_06375"
                     /pseudo
     CDS             complement(2117..3187)
                     /gene="ricT"
                     /locus_tag="PKH68_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     assembly_gap    3268..3367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3368..4419)
                     /locus_tag="PKH68_06380"
     CDS             complement(<3368..4419)
                     /locus_tag="PKH68_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HNX12599.1"
                     /translation="MFSHITGQHAIKERLIRSVREQRIPHAQLLHGPEGVGKLALAVA
                     FAQYISCNHRTQTDACGVCPSCVKYNQLAHPDLHFVFPIIKPAGKTSMVCDDFIADFR
                     KMYINNPYFSVNDWYEKIGEGAKQGMIYANESSEIIRKLSLKTYESQYKVMIIWLPEL
                     MNIDCANKLLKILEEPPEFTVFLLVTNNPDENLTTILSRTQHVKVPSLEEHEIVEGLK
                     DKYPQSAITDIENAARIAGGSFLTAQKLLTETRESSVNFDRFVEIMRLAWLVGNKKDH
                     QSLKTLKKWSETMASADVGRERQKKFLNYAQTMVRENFIYNLRQPALNYMTDYESGFS
                     AKFSPYINERNVEDLVA"
     gene            complement(4621..6318)
                     /locus_tag="PKH68_06385"
     CDS             complement(4621..6318)
                     /locus_tag="PKH68_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX12600.1"
                     /translation="MSKKIMLVASLALAVVFVSCNKNMKALDASYFKTTPNPLEEKGG
                     KVDATVTGTFPVKYFNKKAVVTVTPVMKFNGEEVKAQSSVFQGEKILDNNQKIGYKAG
                     GTYTMKPSFFYIPEMAKSELFLNFSVVQKKKTYNLPAVKVADGVLATSELASVKEIKP
                     ALSADKFQRITQENQNADILFLIQQAQLRNSEIKAADVQAFSSKVKEARDAQNKEVTS
                     LEVLGYASPDGSIDLNTNLAEKRQKVTADFVNKELKKLKSNVSIDSKFTAEDWEGFQA
                     LMESSNIQDKDLILRVLSMYQDPEQREREIKNLSTTFKSIATDILPQLRRSRMKLTVD
                     VTGKSDEQIAQLAAASPADLTVEELLYAATLSKTNSEKAVIYQKAIDLYPSDVRGYNN
                     LGLVNYEMGKVAEAQNLYQKALTVDPQSADANYNLGLIALSKGSFTDAQSYFGKSAGT
                     SGDLNAALGTSYIAQGDYSKARNVMAEVNTNNAALAQILSSDYNSARRTLAAVAAPNA
                     MTAYLSAIIGARTNDRDAVYNNLKTAVQKDSSLAKKALSDLEFAKYFTDASFLSILK"
     gene            6504..7517
                     /locus_tag="PKH68_06390"
     CDS             6504..7517
                     /locus_tag="PKH68_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX12601.1"
                     /translation="MTFNHSYYLFRVLKTGLTARKILNATKLTLSYRLFLAGFRCRWK
                     LSPQFISIEPTNVCNLHCPECPVGTHSGRIERENADENMVFKLLDELQSTLMHVIFYF
                     QGEPLMNRNFTNLVRYAHRKNILTSTSTNAQLINSETAKKIVESGLDRLIISIDGATQ
                     ETYEAYRVGGRLDKAIEAVENLIKWKKVLKSPSPFIEIQFLVLKTNEHQLKEMKLLAR
                     KLKADKLTFKSAQLYDFENGNPFLPNNKRYSRYEQKPDGRYYIKNPLRNRCRRMWYGA
                     VVTTRGDVLPCCFDKDSSFVFGNLKDKSFLECLNGIPAAEFKEAIRKNRRQFEMCRNC
                     TEK"
BASE COUNT         2012 a         1615 c         1661 g         2412 t
ORIGIN      
        1 acatcctgtg gtgtggtaac ggctattgca ccggtaatgc ccatagtctg aaccagggta
       61 agatggatat cgcctgtgcc gggaggaaga tccataataa agaaatcaag ttcgccccaa
      121 acggtatccg tgataagttg ctttaaggca ttgctggcca tactgccgcg ccataccagg
      181 gcgttggcgg gatcaacaaa aaaaccgatg gaaagcagtt taacaccata tctctcaata
      241 ggttgcatca gttgacgacc gtcaatttct tctacaaaag gacgttcgtt ttccacaccg
      301 aacattttgg ggatagaagg gccgtgaatg tcagcatcaa gcaaaccgac tttgtaaccc
      361 attgatgcca gtgctattgc caggttcgat gaaacggttg attttcccac accgcctttg
      421 cccgaaaaaa cagcaattat attcttcaca tccggtagaa tggatgccgg tttgggtttg
      481 ggtaattctt tcgttatgat gctgatatta cctttaatgt ccacgtcttc tccaatgtat
      541 gtgattattg cctgttcggc tgctttcact actgacttgg caaaggggtc attgggttta
      601 tcaaaaagga tggaaaaact aactttatta ttttcaatac gcatattatc ctgaaccatt
      661 tcagcgctta cgatgtcttt cccggtaccc ggataacgaa catgctttaa tgcttcaaga
      721 attaaatttg gataaattgt catcctgttt tataatttta aaagtgtaat actgtttgtt
      781 gtttttattt aacaaacgaa agcgattatt gttgacgcct gaagcgttta aaactgcgat
      841 aaatatccgg ttcgatattt atttccggtt caatcagcac tttacctgct gcgggacgaa
      901 atgctatata tttaatagtc agccctctgt cgagccattg ttgctcgtaa taggttttta
      961 tgccaagcac gggatttttt tcattttcac cggcatatat atcatcgaca tggctcagca
     1021 cttcaaagtc atttagcttg accatttcgc aggtataggt gaacatgaaa ttactatcgg
     1081 tcttgagatg tacaattcct gatggtttca gaaattggag atatgaccgg atgaaattgg
     1141 ttgctgtgag ccgtttggtg gttttcttca tctgcggatc ggggaaggtc agccatatct
     1201 cactgacttc gccgggtgca aaaaaatgaa aaatcatttc aatgttggta cgaataaatg
     1261 ccacattttt cagttggtta acaaaggcat ctttagctcc tgaccagata cgggcacctt
     1321 ttatatccac tccgatgtag tttttatccg gaaatcgcct ggccagctct acggtgtatt
     1381 cccctttacc gcaacccagt tcgagtacta tcggattggg gttttggaaa tactgttcgt
     1441 tccattttcc ctttaactca aatccctcac caccgagaag cacaccggat ttaacctgaa
     1501 acacattggt gaaggtttct aattcggcaa atttggctaa tttatttttt cccatactca
     1561 gtttgctttc tccacccgta ttccaatgtc attaattcct acaaggatgc tgtcgcgcat
     1621 tccctgtcct attttcatct gataaatgcc gctgtcagga aaaatgacct gctgacggta
     1681 aagtaccggc atttctttga gcgctccggc accggtgccc agccattttc caaattcgtc
     1741 ggccaaaaaa cattcaatgg tatctttctg tatcattcct ttcatatctt tattttccag
     1801 gaaaagccac aggttttgat agggataatt gccgtgatga cgaacattga tatacagatt
     1861 gtaaggcagg gttttatccc ggatagcgac attataggtg agcaggctgt ccttgttcca
     1921 accactcgcc gacacggtct tatagctgaa atagatgtct ttcttattac acgaaagcac
     1981 agccacaccg ataaatatca aaacaatata tttactgatt tcctttatca tttctttttt
     2041 tgagtgtttt gggggttgaa tttgcagggt ttttaggtcg tgccggacgt tgactccgat
     2101 tctgactgga tgtatttttg tttttaacat caaatcgtgt cagactgtcc tgtcctacta
     2161 catttccata ttctacgggc tttttttctt cattcaacga aacatccagc tgtagcggtt
     2221 tattaccttt tttattcatc attatgattt ctctggcacg ttccgccgaa atggtgacca
     2281 gatttgcccc gaaattggga gaagttgaat acgtaatcaa tcccttgaaa atatcggttt
     2341 tgaagtggaa atagactgca tccttggttt cgagttgcac ttcgcgtgaa ggcaggtgct
     2401 tgttggcatc catgtatgca tccagttcga aattcatgca acatttgagt ttagcgcatt
     2461 gtccggccag tttttggggg ttgagcgaca aatcctgata acgggcggcg ctggtggata
     2521 cggtgctgaa gttggtcatc catttggagc agcacagttc gcggccacaa gggccaatac
     2581 caccgatccg gccggcttcc tgacgggcac cgatttgttt catttcgatt cgaacccgga
     2641 atgtctcagc aaagactttt atcaactggc gaaagtcaac ccgctcgtcg gcaatgtagt
     2701 agaagatggc cttgttgcca tcgccctgat attccacatc gcctattttc atgttaagat
     2761 gcagactttc ggcaatctgc cgcgatttga tcatggtttc ctgctctttg gctttggctt
     2821 cctgatactt ttccatgtcc gtttcacgca ctttacgata cacccgtcgg atttcaaatt
     2881 tatcgaaatc gatgttgttt tttttcatct gcagggtaac caacttaccc accagcgtta
     2941 cctcaccgat atcgtgtccg ggtgatgctt ccacagccac catatcgcct ttttcaagtt
     3001 caattttggt attattcaga aagtaacttt tccgggtatt tttaaattgc acttccacga
     3061 aatctgtttc gctgatggtt tcgggaagat cgcacagcca gtcgtgcaca ccgagttttt
     3121 gacgggtggt gttacggcag cccttcttat tcatccggca tccgccggta tctaatctgt
     3181 attccatgtg tagtcggttt tattcttaaa aacaattctc atttcccatt actttttttc
     3241 agaaaataaa atcacgtttc atgctttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnngca accagatctt ccacattccg ttcgttgatg tagggtgaaa atttggccga
     3421 aaagccgctt tcatagtcgg tcatataatt cagtgcgggt tgacgaaggt tgtaaataaa
     3481 attttcacgc accatggttt gtgcataatt taaaaatttt ttctgccgtt cacgccccac
     3541 atcggcactt gccatcgttt ccgaccattt tttcagcgtt ttgagcgatt gatgatcttt
     3601 tttatttcct acgagccagg ccagccgcat gatttcaaca aaccggtcga aattgaccga
     3661 actttctctg gtttctgtca acaacttttg cgcagtcaga aagctccctc cggctattcg
     3721 ggcagcattt tcaatgtcag taatagccga ttgcggatat ttatctttaa gcccctccac
     3781 aatttcatgc tcttcgagtg agggtacttt cacatgctgg gttctggaga gtatggtcgt
     3841 caggttttcg tccggattat ttgttacaag gagaaaaacg gtgaattcgg gtggctcttc
     3901 cagtattttc aggagcttat tggcacaatc aatattcatc agttcgggca accaaataat
     3961 catcacctta tactgcgact cgtaggtttt caaactcaat ttccggataa tttcgctgct
     4021 ttcattggca taaatcattc cctgcttggc accttcgcct atcttttcat accagtcgtt
     4081 aacgctaaaa taaggattat ttatgtacat tttccggaaa tcggcgataa aatcgtcaca
     4141 taccatggaa gtttttccgg cgggtttgat aatggggaaa acgaaatgta aatcgggatg
     4201 agccagctga ttgtatttga cacatgaagg acacactccg caagcatcgg tctgtgtgcg
     4261 atggttacag gatatgtatt gagcaaacgc aacggctaaa gccagtttac caacgccttc
     4321 agggccgtgg agcaactggg catggggtat gcgctgttcg cggacggagc ggatcaatcg
     4381 ctccttaatt gcatgttgtc ctgttatatg gctgaacact taacttttta tttttcaaag
     4441 gtcaaaaaaa ctttcacttt ctgcgaaagc agattatccc aatttgtatt atagcacaaa
     4501 ggtacaagaa taaattttta attccataaa gttacagaat tttttaagaa cccgaaaact
     4561 gggccataaa aaaaccccga taccagacgg tatcagggtt taagaagtta tttcaatata
     4621 ttatttcagg atggacagga agctggcatc ggtgaaatat tttgcaaatt ccaggtcgct
     4681 gagagctttt ttggccagtg aactgtcttt ctgaaccgct gttttcaggt tgttgtacac
     4741 ggcgtcgcgg tcgttggtgc gggcaccgat gatggcgctc aggtaagctg tcatggcatt
     4801 gggagcagcc actgcagcca gtgtgcggcg tgctgaatta taatcagaac tgagaatttg
     4861 cgccagtgct gcgttgttgg tatttacctc agccatcaca ttacgggctt ttgagtaatc
     4921 gccttgtgca atatagcttg ttccaagagc agcattcagg tcaccggaag ttcctgccga
     4981 tttaccaaaa taagactgag catcggtaaa tgagccttta gaaagggcaa taagacccag
     5041 gttgtaattg gcatcggccg attgcggatc aacggtaaga gctttttggt agaggttctg
     5101 tgcctctgct acttttccca tttcgtagtt tacaagtccc aggttgttgt agccgcgtac
     5161 atccgaggga taaagatcaa tcgctttttg atagattact gctttttcag aattagtttt
     5221 ggagagtgta gcagcgtaaa gcaattcttc tactgtcagg tcggcaggcg aggcagcagc
     5281 cagttgagcg atttgttcgt cggatttacc ggttacatct acggtcagtt tcatgcgtga
     5341 acgacgcaac tgtggcagaa tatcggtagc aatagatttg aaggtggttg acaggttttt
     5401 aatttcgcgt tcgcgttgtt cgggatcctg atacatggag agtacacgca ggataaggtc
     5461 tttgtcctga atgttggaac tttccatcaa tgcctggaag ccttcccagt cttcggctgt
     5521 aaatttgctg tcgatcgaca cattcgattt gagttttttc aattctttat ttacaaaatc
     5581 agcagtaact ttctgacgtt tttcggccag attggtattc aggtcaatgg aaccatcggg
     5641 agatgcataa cccagtactt cgagcgatgt tacctccttg ttttgggcat cgcgggcttc
     5701 tttaactttc gaagaaaatg cctgcacatc ggcagctttg atttctgaat tgcgcagctg
     5761 agcttgctga ataaggaaaa gtatgtcggc attctgattt tcctgagtaa tacgttgaaa
     5821 tttgtctgcg gaaagtgccg gttttatttc ctttacagaa gccagttcgg aggtggcaag
     5881 aacaccgtca gctactttaa cagccggcaa attataggtt tttttctttt gaaccacact
     5941 gaagtttaga aaaagttcag atttagccat ttcgggaata taaaagaagg atggcttcat
     6001 ggtgtaagtg ccaccggctt tgtatccaat tttttggtta ttgtcaagaa ttttttctcc
     6061 ctgaaatacg gatgactgtg ctttcacttc ttcaccgttg aatttcatga cgggtgtaac
     6121 tgtaactact gctttcttgt tgaagtactt taccgggaaa gtgcctgtta ccgtagcatc
     6181 gacttttccg cctttttctt caagcggatt aggagttgtt ttgaaatagc tggcatccag
     6241 tgctttcatg tttttgttac aggaaacaaa aacgacagcc aaagccagac ttgcaaccag
     6301 cataattttt ttgctcatag ttctaattgt ttgatattta atagtttatt attttcataa
     6361 atccgaaaca aagatacatt tttaatttta tttttactta ctttgccgtt ggattttgac
     6421 ttttttgatt tgatacaata attattcaca ttttcaatct gcatgacttt gttttttaaa
     6481 gacttactaa ttttatattc tgcatgactt tcaatcatag ttactattta ttcagggtat
     6541 tgaaaacagg attgacagcc cgaaaaatac ttaatgctac gaaattgacg ttgtcgtacc
     6601 ggcttttttt ggcgggcttt cggtgcaggt ggaaactgtc gccacagttt atctccattg
     6661 aaccaaccaa tgtatgcaat ctgcattgtc ctgaatgccc tgtggggacg cactccgggc
     6721 gaattgaaag agaaaatgcg gatgagaaca tggtctttaa actccttgac gaacttcaat
     6781 ccacactgat gcatgtgatt ttttattttc agggcgaacc gttgatgaac cggaatttta
     6841 caaatctggt acggtatgca caccgtaaaa atatactgac aagcacttct accaatgctc
     6901 aactgataaa ttctgaaact gcaaaaaaaa tcgtagagtc gggactggac cggcttatta
     6961 tttccatcga cggagctacg caggaaacgt acgaggcgta tcgggtcggc ggacggctgg
     7021 ataaagcgat tgaggctgtt gaaaacctga taaagtggaa aaaagtgctg aaatcgcctt
     7081 cacctttcat tgaaattcag tttctggtac tgaaaacgaa cgagcatcag ctgaaagaaa
     7141 tgaagctgct tgcccgaaaa ttgaaagcag acaaactgac ctttaagtct gcacaacttt
     7201 atgattttga aaatggaaac ccgtttttgc ctaacaataa gcggtatagc cgctacgagc
     7261 agaaaccgga tgggaggtat tatatcaaaa atccgttgcg caaccgctgt cgccggatgt
     7321 ggtacggagc cgtagtaact acgcgtggcg atgtgctgcc ttgctgtttc gacaaagaca
     7381 gctctttcgt gtttggcaat ctgaaagata aatcgttttt ggaatgcctg aatggcattc
     7441 ccgcagcgga attcaaagaa gcaatccgga aaaaccgcag gcaatttgag atgtgtcgca
     7501 actgtacgga aaaataatta ccccaaaatt ccctgaaggt gaatattata gaacttctat
     7561 atttcgtgat tttactttca ataaaaaatg tcctgtaaga aatttaatca gaatattaaa
     7621 aattttaaag ttggctcttt aagtattttt gatttttttc gaataaccaa tcaaaaaagt
     7681 tgaattcctg ttaaccggac cgttaaattt tcagtgcctg ccttgggcaa agtgcatgac
     7741 gttggcggga ggtcgctcat aatgaagttc attagccata aattccatgt aaggcaaggc
//
LOCUS       DAOPZK010000088         7768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_11292_length_7768_cov_24.762998, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000088 DAOPZK010000000
VERSION     DAOPZK010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11292_length_7768_cov_24.762998"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /gene="guaB"
                     /locus_tag="PKH68_06395"
     CDS             <1..1379
                     /gene="guaB"
                     /locus_tag="PKH68_06395"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX12602.1"
                     /translation="LTTRFTRNIELKIPIVSAAMDTVTEAKMAIAIAREGGIGVIHKN
                     MSIAEQAKQVSIVKRAENGMISNPVTIKKGATVGDALSLMAEYKIGGIPVVDDEGYLV
                     GIVTNRDLRFQRDIHKLIDEVMTKENIVTTTRTTNLEEAADILQNHKIEKLPVVDEDN
                     KLIGLLTYKDITKAKDKPMACKDSRGRLRVAAGVGVTSDTFERIEALVEAGVDAIVID
                     TAHGHSKGVIDTLREAKKRFPTIDIIVGNIATAEAALALAEAGADAVKVGIGPGSICT
                     TRVIAGVGVPQLTAIYGVSKALAGTGVPVIADGGIRYSGDIVKALAAGANTIMAGSLF
                     AGVEESPGETIIFSGRKFKSYRGMGSLEAMEKGSKDRYFQDMEDDIKKLVPEGISARV
                     PYKGSLYEVIYQMIGGLRAGMGYCGSHNITELHKAKFTRITSAGIVENHPHDVTITSE
                     APNYSRGE"
     gene            1530..>2992
                     /locus_tag="PKH68_06400"
     CDS             1530..>2992
                     /locus_tag="PKH68_06400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX12603.1"
                     /translation="MKKIFVSVFGLFLAVANLTARPSDPTLMKINGKDIPLSEFEYIY
                     NKNNTNNVLDKKTLDEYVDLFVNFKLKVEEAIAQGLDTTQSFNSELSLYRNQLAEQYL
                     TDSDMNAALLQEAYDRKKEEVEVSHILVKIADTGTAADTLAAYNKALSIIKRAQKEDF
                     AKLAKEVSDDQYASKTGGYVGWVNALRTPYSFENAAFATQAGSVSKPVRTFLGYHIIK
                     VLDRRPSQGEVHVKHIFLLDNDPNSTNKAELKARIDSIYQRLKAGDDFGELAKKHSQD
                     PGSARQNGELPWFGSGQMIPEFEKAAFALKQEGEYTEPVHTQVGWHIIQLIGKRPLAG
                     MDELKSDLENQIKANERSQKIMNSFTGKLKKEYNFTMNEAAATEIYQLADKLAPSDSL
                     FNVEMNKLDKPLFTISDKSFTQKDFSNYMLPAAAGFRGVRSDFIKEQLNAFINNKLST
                     YEQTQLDRKYPEFHYLMQEYHDGILLFEVSNREVWEKA"
     assembly_gap    2993..3092
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3355..4200
                     /locus_tag="PKH68_06405"
     CDS             3355..4200
                     /locus_tag="PKH68_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX12604.1"
                     /translation="MKKFFFFILTISALACSDKGGDIKRTPVLEVEGKFLYQDEIDQI
                     IPATASLIDSADIAERYIKKWVTDVLVYENAKRNINKLNDIEKRVEEYRKSLIINEYE
                     QALIKERLSGVVTEDEMKKFYETYKSQLISQENLIKGLLLIVPKNAPQIDKVREWVRL
                     SNTKSLENIEKYSLKNAISYDYFMNNWTSFGEILKKAPFRIEDSGVFVRSTSFAETSD
                     STKMYLLRIVSSIPTGQTEPYDLAKKKISSILLNKKKSDFIIQFENDIYKDAKEEGSI
                     NYFKK"
     gene            4306..5661
                     /locus_tag="PKH68_06410"
     CDS             4306..5661
                     /locus_tag="PKH68_06410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX12605.1"
                     /translation="MRKISLILIAFLLIFSGLSAQKKNIIDEVIWIVGDEAILKSDVE
                     AVLLDAEMRRQPIQGNAYCIIPEQMAIQKLFLHQAALDSVTVNEGYIASLAEADLNKY
                     IADVGSVEKAEEYSHKTVAQLREIIRDKYKSQALIEQMQSQLVSGIQATPAEVRRHFS
                     KIPQDSLPTIPEQVELQVLSVNPPIPLTEVNRVKEKLREFTERATANPNDFSLLARLY
                     SEDPGSAVKGGELGFTARATLDPEFAAVAFNLQDPKKVSRIVESQFGFHIIQLIGKRG
                     DKVNCRHILLKPRASIEDKQKGINRLDSLGDLIRQNKITFEQAVMHYSMDRNTAMNAG
                     LMVNPNTGNSKFEYQDLPAEVAKAAYSMNIGEVSKAFTMIDPLTGKEMVTIVKLKSKT
                     PTHKANIMDDYQILKNILEGNKKQEFLHNWISKKQKETYISIKPEWRDCNFQYKGWIK
                     E"
     assembly_gap    5726..5825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5826..7463
                     /locus_tag="PKH68_06415"
     CDS             <5826..7463
                     /locus_tag="PKH68_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNX12606.1"
                     /translation="ENSDALFFDQVMFPDIQVLKGNVRFRHENAVLHCDSAYFNDVNN
                     TFDAFGRIKMVQGDTLFIYGDYLNYDGNTRLARLRDNVKMVNRNTTLTTDSMNYDRTA
                     NLAYYYTGGKVVDDVNVLTSVWGQYSPATRQALFKDDVKMTNPNFTLTSDTLKYNTKT
                     SIADIVGDSHVLYEEKTNIYSKLGWYDTAKDRMMLLDRSFVEQNDGKTLVGDTIFYDK
                     KNKYSEAFSNVELNDPKQKTTLYGNFVSYDEAKEKGLATDSALLIDWSGKDSLFLSAD
                     TLYNFKDSIYDAVKAQKNVRFFRVDVQGMCDSLFYTSRDSIMHLTALPVLWSDNNQLM
                     GDKISAYTKNKKVEKIHIDQSAMAIQKDTLDYYNQLSGKEITAYLDSNQLRRVDVDGN
                     AETIYFPRDEKTREIIGLNKTVSSYVRMYLKDKKVERIILTTASSGSMYPIIDLAEND
                     LYLRNFYWYETERPSKMEDVFLKFARTAPPKRTESEKKPGFPTEGGEETDKQAKQNTD
                     RSTNNSVNRNTNNSSNRNTGRQSINTQRSRGSNIKIQ"
     gene            complement(7520..>7768)
                     /locus_tag="PKH68_06420"
     CDS             complement(7520..>7768)
                     /locus_tag="PKH68_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HNX12607.1"
                     /translation="YFVIMFITSFWMSKKAGATYEQSTTLSFTAASNNFELAIAVAIA
                     VFGINSGEAFAAVIGPLVEVPVMIGLVNVAFAFRKKWF"
BASE COUNT         2478 a         1692 c         1628 g         1770 t
ORIGIN      
        1 atctcaccac ccgatttacc cgaaatattg aactgaaaat acccattgta tctgctgcca
       61 tggatacagt taccgaagcc aaaatggcca ttgccattgc acgggaaggc ggcatcgggg
      121 taattcacaa aaacatgtcc attgccgaac aggcaaaaca ggtaagcatt gtgaaacggg
      181 ccgaaaacgg aatgatttca aatcccgtga ccataaaaaa aggagcaacc gtgggtgatg
      241 ctctgagtct gatggctgaa tacaaaatcg gtggaatacc cgttgtcgat gatgagggtt
      301 atctggtcgg aattgtaacc aaccgcgacc ttcgctttca gcgcgatata cacaaactga
      361 tagacgaagt gatgaccaag gaaaatatag ttacaacaac ccgtaccaca aaccttgaag
      421 aagctgctga tattctccaa aaccataaaa ttgaaaaatt gccggtagtt gacgaagaca
      481 ataaactgat cgggctgctt acctacaagg atattaccaa ggccaaagat aaacctatgg
      541 cctgcaaaga cagccgcgga cgactgcggg tagctgccgg tgtgggtgtc acatccgaca
      601 cattcgaacg aattgaggct ctggtggaag ccggtgtcga tgccatcgtt attgatacgg
      661 ctcacggaca ttcaaaaggt gtaattgaca cacttcgtga ggcaaaaaaa cgatttccaa
      721 ccattgatat aatcgttggg aatatagcta ctgccgaagc agcacttgca ctggcagaag
      781 ccggcgccga tgccgtaaag gtaggaattg gtccgggttc catctgtacc acccgcgtta
      841 ttgccggtgt gggtgttcct cagcttacgg ctatttacgg agtttcgaaa gctcttgcag
      901 gcacaggagt accggtgata gccgatggag gcatccggta ttcgggagat atagtgaaag
      961 cactcgcagc gggtgccaac accattatgg ccggatcgct gtttgcaggt gtggaggaat
     1021 caccgggcga aaccatcatc ttcagcggcc gtaaattcaa atcgtaccgc ggtatgggat
     1081 cactcgaagc catggaaaaa ggctcaaaag accgctattt tcaggatatg gaagatgata
     1141 tcaagaaact tgttccggag ggaatttccg ccagggtgcc ttacaagggt tcgttatatg
     1201 aagtgatata tcagatgata ggcggactgc gcgcaggaat gggatactgt ggttcacata
     1261 acatcaccga actgcacaag gccaaattca cacgcatcac cagtgccgga attgttgaaa
     1321 atcatcccca cgacgtgact atcaccagtg aggctccaaa ttacagtcgc ggtgaataat
     1381 cttattaatc accaaaaaaa ataaaacact caaattactc tcttagccgg gagtaatttt
     1441 ttgtgtaaaa atgaattgtt tcacaatttt aatgcttaat catcgttttt tttatcagct
     1501 aaaaaaataa attcaaaatt ttaatccgta tgaaaaaaat ttttgtttct gtctttggac
     1561 tttttctggc tgttgccaac cttacagccc gaccttccga ccctactctg atgaagatta
     1621 acgggaaaga tattccactt tcggaatttg aatacattta taataaaaac aatacgaata
     1681 acgtgttgga taagaaaacc ctggatgaat atgtggattt gtttgttaac ttcaagctga
     1741 aggtggaaga agccatcgct cagggcctcg ataccacaca atcattcaat tcggaacttt
     1801 cgctctaccg caatcaactc gccgagcaat acctcaccga cagcgacatg aacgctgctc
     1861 tgctgcagga ggcttacgac cgcaaaaaag aagaagtgga agtgagtcat attctggtga
     1921 aaatagccga taccgggacc gcagccgaca cacttgccgc ctacaataaa gcactgagca
     1981 tcatcaagcg tgcacagaaa gaagatttcg ccaaactggc caaagaagtt tcggacgatc
     2041 agtacgcatc caaaaccggt ggatatgtag gctgggtaaa tgcccttcgc actccctact
     2101 ccttcgaaaa tgctgccttt gctacacagg caggctcggt atccaaacca gtacggactt
     2161 ttctgggtta tcacatcatt aaggtactgg acagaagacc ttcacagggc gaggtgcacg
     2221 tgaaacacat ctttttgctc gacaacgatc caaattctac caacaaagct gaactgaaag
     2281 ccagaataga ttcaatttat caacgactga aagccggaga cgatttcggt gagctggcta
     2341 agaaacactc ccaggatccg ggttcggcac gccaaaacgg tgaacttccc tggtttggca
     2401 gcggacagat gataccggaa tttgaaaaag ccgcttttgc gctgaaacaa gaaggcgaat
     2461 ataccgaacc tgtacacact caggtcggat ggcacatcat tcagttgata ggtaaaagac
     2521 cacttgccgg aatggatgaa ctcaaatcag acctggaaaa tcagataaaa gccaacgaac
     2581 gttcacagaa aatcatgaat tcatttaccg gtaaactgaa aaaagaatac aacttcacga
     2641 tgaacgaagc tgccgcaacc gaaatctatc agcttgccga caagctggct ccctccgact
     2701 ctcttttcaa cgtggaaatg aataaactgg ataaacctct gttcacaatc tccgacaaat
     2761 catttaccca aaaggatttc agtaattata tgctgcccgc tgcagccggt ttccgcggtg
     2821 tacggagcga ttttatcaaa gagcagctca atgctttcat caacaacaag ctgagcactt
     2881 atgagcaaac ccagctggac agaaaatatc cggaattcca ttaccttatg caggaatatc
     2941 acgacggtat tctgctgttc gaagtaagca accgcgaagt gtgggaaaaa gcnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctacttta tcagtggcaa agtactcaaa
     3121 acaatgcccg aagactatac cgatgtacgg ggtgtgatta ctgccgatta tcaggattat
     3181 cttgaaaaag aatggattaa aacgctccgg cagaaataca cattcactgt tgacgaaaaa
     3241 gtactgaaaa cggttaagaa aaactaatta attaacgatt gccaaaataa aagttctacc
     3301 tttgaacggt tattttggca tcgttatgta ttgaaatgac tgtattttaa gctaatgaaa
     3361 aagttttttt tcttcatact caccataagt gctttagctt gttctgataa aggcggcgat
     3421 atcaagcgaa caccggtatt ggaagtggaa ggaaaatttc tgtatcagga cgaaatagat
     3481 cagatcattc ctgccacagc ctcccttatt gacagcgcag atatagccga acgttacatt
     3541 aagaaatggg taacagatgt attggtgtat gagaatgcca aacgtaacat caataagttg
     3601 aacgacatag aaaaacgggt ggaagagtat cgtaagtcgc tcatcatcaa cgaatatgag
     3661 caggcactca tcaaagagcg cctttcgggc gtagtgaccg aagatgaaat gaaaaaattc
     3721 tatgaaacct ataaatctca actcatcagt caggaaaatc tgataaaggg tttattgctc
     3781 atagtgccca aaaacgcacc acaaatagac aaagttcgcg aatgggtcag acttagcaac
     3841 acaaaatcgc tggaaaacat tgaaaaatac agcctgaaga atgccatcag ctacgattat
     3901 ttcatgaata actggacttc attcggcgaa atactgaaaa aagctccctt cagaattgaa
     3961 gattccggag tatttgtcag atcaacatca ttcgccgaga catccgacag tacaaaaatg
     4021 tatcttctcc gaattgtgag ttcgataccc accggacaaa cagaacctta cgacctggct
     4081 aagaaaaaaa tatcatccat cctgctaaac aagaagaaat cggactttat cattcagttc
     4141 gaaaacgata tttacaagga tgcaaaggaa gagggctcca ttaattactt taagaaataa
     4201 aaaaatacga tttcactgcc ggcaggcacg gatcatcgct caaaaaagac atattcatta
     4261 aacaaatccg ctaactaaac ccggttatcg gctaaaaaag aaaaaatgcg taaaatatca
     4321 ctgattttaa tagctttcct tttaattttt tccggtttat ccgctcaaaa gaaaaatata
     4381 attgatgaag taatctggat tgtgggtgac gaagccattc tcaaatccga tgtggaagct
     4441 gttttgcttg atgcagaaat gcgccgccaa cccatacagg gaaatgccta ttgcatcata
     4501 cccgagcaga tggccattca gaagctattc cttcatcagg ctgcactcga cagtgttaca
     4561 gtaaatgaag gttacatagc atcgcttgcc gaagccgatc tgaacaaata cattgccgat
     4621 gtggggtcgg tagaaaaagc cgaggaatat tcacacaaaa cggtagcaca gttgcgtgaa
     4681 atcatccgcg acaaatataa aagtcaggcg ctgatagaac agatgcaaag tcagctggta
     4741 agcggcatac aggctacacc cgccgaagtt cgccgccatt tcagcaaaat accacaggac
     4801 agtctgccca ccatccccga acaggtagaa ctgcaggttc tgtcagtgaa tccacccata
     4861 ccacttaccg aagttaaccg tgtgaaagaa aaactgcgtg aatttaccga acgcgccact
     4921 gccaatccca atgatttcag cctgctggca cgcctctact ccgaagatcc cggaagtgcc
     4981 gtgaaaggcg gtgaactggg ttttaccgcc cgtgccaccc tcgatcctga atttgcagct
     5041 gtagccttta acctgcagga tccaaaaaaa gtttcacgca ttgtagaatc tcagttcgga
     5101 tttcacataa ttcaacttat cggcaagcgt ggcgacaaag tgaattgccg gcacatcctg
     5161 ctcaaaccgc gtgcatcaat cgaagacaag caaaaaggga ttaaccggct ggattcgctg
     5221 ggcgatctta tccgtcagaa taaaatcacc tttgagcaag ccgtaatgca ttactccatg
     5281 gaccgtaaca ccgccatgaa tgccggactg atggtaaatc caaacaccgg aaattccaaa
     5341 tttgaatatc aggaccttcc tgccgaagta gccaaagcgg cttacagcat gaatatcgga
     5401 gaagtgtcca aagcgtttac gatgattgat ccccttaccg gaaaagaaat ggtgaccatc
     5461 gtaaaactaa aaagcaaaac tcctacacat aaagccaaca ttatggatga ttatcagata
     5521 ctgaaaaata tcctggaagg caataaaaaa caggaatttt tgcacaactg gatctccaaa
     5581 aaacaaaaag aaacctatat cagcatcaag cccgaatggc gcgattgcaa cttccagtac
     5641 aaaggctgga ttaaagaata gattatttca ctgtattttc gatgacaata aacctacgaa
     5701 atactgatct gatcagggtt ctgatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnngaaaa cagtgatgca cttttttttg atcaggtgat gtttcccgac attcaggtgt
     5881 tgaaagggaa tgtgcgcttc agacatgaaa atgccgtact gcactgcgac agtgcttatt
     5941 tcaatgatgt gaacaacacg tttgatgcat tcgggcgaat aaaaatggtg cagggtgaca
     6001 cactgtttat ttacggcgat tacctcaact acgacggaaa cacccgcctg gcacgtctgc
     6061 gcgataatgt gaaaatggtg aaccgcaaca ccacgctcac caccgatagc atgaactacg
     6121 accgcaccgc caatctggct tattactaca ccggtggcaa agtggtggat gacgtcaatg
     6181 tgcttacctc tgtgtggggt cagtactccc ccgctacccg acaagcacta ttcaaggatg
     6241 atgtgaaaat gaccaatccc aatttcacac tcacatcaga taccctgaag tataatacca
     6301 aaacttcgat tgccgatatt gtgggcgact cacatgtgct ctacgaggaa aaaacgaata
     6361 tctacagtaa actgggctgg tacgatacgg ccaaagaccg tatgatgctt cttgaccgat
     6421 cgtttgtgga acaaaacgat ggaaaaacgc tggtgggtga caccattttt tacgataaaa
     6481 aaaataagta tagcgaagct ttctccaatg tcgaactcaa cgacccaaag caaaaaacca
     6541 cgctttacgg caatttcgtg tcgtacgatg aggcaaaaga aaagggtctg gcaacagact
     6601 cagcgctgtt aatcgactgg tccgggaaag actcactttt tctcagtgcc gatacgctct
     6661 ataatttcaa agactccatt tacgatgccg taaaagctca gaaaaatgta cgattttttc
     6721 gggtggatgt tcagggaatg tgcgattcgc ttttctacac ttcgcgcgat tccattatgc
     6781 acctcacggc tttgccggta ctgtggtcgg acaataatca gttgatgggt gataaaattt
     6841 cagcttacac caaaaacaaa aaagtagaaa aaatacatat tgatcaatcg gccatggcca
     6901 tacagaaaga tacgctcgat tattacaacc agttgtcggg aaaggaaata actgcttatc
     6961 tcgatagtaa tcaactacgc cgtgtagatg tggatggaaa tgccgaaaca atttattttc
     7021 ccagggatga aaaaaccagg gagataatcg gattgaataa aacggtgagc agttatgtac
     7081 gtatgtatct gaaggataag aaagtggagc gaataattct caccaccgct tcgagcggtt
     7141 caatgtatcc gatcattgat ctggcagaaa atgacctgta tcttcgaaac ttttattggt
     7201 acgagactga aaggccttct aaaatggaag atgtgtttct aaaatttgcg aggacagctc
     7261 cacccaagcg aactgaaagt gaaaagaaac ccggatttcc taccgaagga ggcgaagaaa
     7321 cggataagca agcaaaacaa aacactgaca gaagcaccaa caactccgtc aacagaaata
     7381 caaacaacag ctcaaaccgt aataccggaa ggcaatccat aaacacccaa cgatcaaggg
     7441 gcagcaatat aaaaattcag taaatcaaca tatcaaattg agacagataa cccgccaaat
     7501 cgacgggtta tctgtttttt cagaaccact tctttctgaa agcaaaggca acattcacca
     7561 ggccaatcat taccggaact tccaccaacg gaccgataac agcggcaaat gcttcacctg
     7621 aattaattcc aaaaacggca atggctacgg caatagccaa ctcaaaatta ttgctggcag
     7681 cggtaaacga cagggtggtt gattgttcgt acgtggcacc cgctttttta ctcatccaga
     7741 atgaggtgat gaacatgatg acaaagta
//
LOCUS       DAOPZK010000089         7726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_11381_length_7726_cov_25.365402, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000089 DAOPZK010000000
VERSION     DAOPZK010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11381_length_7726_cov_25.365402"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PKH68_06425"
     CDS             complement(<1..1037)
                     /locus_tag="PKH68_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX12608.1"
                     /translation="MKKSILMLVIASVLIGCSSNYKKYKSFDEYPVYNGNDLELTYSA
                     TGSKFRVWAPTADEVKLLLFDNGIEGAAYMMKNMHRSKNGTWVVAVSGDLKGKFYTFQ
                     IKTGEKWLNETPGMWVKAVGVNGKRAAIIDFNETNPEGWEKDKRPELKNVADIALYEL
                     HVRDFSMSPNSGMKNKGKFLAFTEQGTKNTAGQATGIDHLKELGITHVHLLPSYDYAS
                     VDETKLNENKYNWGYDPLNYNVPEGSYSSDPTNPVARIKEFKQMVQSLHRANIRVVMD
                     VVYNHTFVGEESHLNLLAPGYFYRFNKDSTWSNASGCGNETASERPMMRKFMIESVVY
                     WAKEYHVDGFR"
     gene            complement(1173..3236)
                     /locus_tag="PKH68_06430"
     CDS             complement(1173..3236)
                     /locus_tag="PKH68_06430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNX12609.1"
                     /translation="MTVITAAGSALAGNPFFKPYNTPYQTIPFNELKNEYYMPSFEEA
                     MKQHRAEIEKIVNNPKPPTFENTIVALDKSGELLSKVASPFYNLLSAETTDEMQKIAE
                     ELSPKMTEHSNSIRLNEKLFARVKAVYEQKDKLKLTPEQQMLLKDTYDGFADNGANLS
                     DADKDVYRELSKELSLLTLNYGQNVLKETNKYQLVITDKELLKGLPADVLDMLAENAQ
                     KAGKTGWLINMKATSYVPVMKYADNRDLRRELYMASASKCMVGGDYDNRENLVKIANL
                     RLKIANLLGHKSYADYALVHRMAENKENVYDLLNKLLAAYKPAAEKEYAEVQAYADKN
                     GAYFKVQPWDWSYYSEKLKAEKYELNDEMTKPYFELEKVKQGIFWLSNKLYGLKFTEN
                     PRIQVYNPEVKAYDVTDADGKFVAVFYADYHPREGKRAGAWMNDMKGQWMEGKKDSRP
                     HIVNVMNFTRPTATKPALLTFDEMTTMLHEFGHALHGMLTKTNYRSLSGTNVYRDFVE
                     LPSQIMENWAVEKEFLDNFAVHYQTGEKMPAELIQKIKDADNFNIGYLALRQLSFALL
                     DMKWHTLEQPFEGDAIAFEKEAMTPAQVLPAIENTAMSPTFSHIFSGGYAAGYYSYKW
                     AEVLDADAYSQFQKNGIFDKKTAASFRKNILEKGGTEHPMVLYKRFRGQEPSIDALLK
                     RNGIK"
     gene            complement(3279..4637)
                     /locus_tag="PKH68_06435"
     CDS             complement(3279..4637)
                     /locus_tag="PKH68_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12610.1"
                     /translation="MGQSKLHILLLLITALLMGAACYSQTDPNPLITEVQVTTADSTY
                     RYLYYYNGDKAKSLETRYVWKGEDWQRRQQTEWIYTGSLCTLQRMSKWINGNWETYYQ
                     IENEYADGKPVKETHQEVQNGIAKFISKSVFTYNQNLLTSKTEYKRVGADWITTLATD
                     YSYNEAGLNELIAIKKYSSGFLSGESRINLTYNDSSLVKTQIYAEKSDTGWVNISLSE
                     TYYKPNSKLKSSEILKLRDETYHTWVNKQNVYYTYDNSGRLTDEIYQNWKYVYWENDL
                     RYRYEYDTQGELLRKTTCLPVYDDFRAVTSVNYSDFQYAKPSLIEARLEFWGGETGAQ
                     ITTFIPYRFNSETVVQQAARVVLSYIPVSETSVKSAEIHRRANEINVYPNPSTGIFYF
                     DTQQHDVSRWTLSDLSGKKLMTKNQTKRSGVIDLGDYTPGIYILQVVTPEGVQVQKLI
                     RE"
     gene            4865..>5427
                     /locus_tag="PKH68_06440"
     CDS             4865..>5427
                     /locus_tag="PKH68_06440"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNX12611.1"
                     /translation="MKKLVIPYITDLETADIESSADILEENGTKSAIDVLNWPDQYPY
                     KPITYFFTGRSADAVFIKYSVKGSMLKAVYTEDHSPVHEDSCVEFFCMPEGADKYTNF
                     EFNCIGTCSASSRKGRSEDVVPFPAEDMVSILRYPSMGHRAFKEMEGMFEWELTVKIP
                     LKLMNLDPDNLPEKIRGNFYKCADDTDS"
     assembly_gap    5428..5527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5528..>7726
                     /locus_tag="PKH68_06445"
     CDS             <5528..>7726
                     /locus_tag="PKH68_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNX12612.1"
                     /translation="THADVFLNGARILQTQNMFVGHQIPVKNSLRAGENSLFIRFYSP
                     VKRMEPARLTAGYEYPAGNDHSDKKVSIFNRKAPYQFGWDWGIRLVQMGIWKPVTLRF
                     YNKAIIDNFYVKQISITKDKAVTDNQIEIYSTSETPAKISTGAGFNSLPVLSVDKEIV
                     LKKGKNIVSIPLTIDNPNLWMPAGWGKQNLYDFSVKVYIGDKLVSEKTEKIGLRKVRL
                     VQQPDEHGKSFYFEVNDIPLFAKGTNYIPGEILRTRQDKAYYNRLFDNLTEANMNFIR
                     VWGGGTYEDDYFYQLADEKGILVWQDFIFGCVPYPSDDAFLANVKEEVIYNVKRLRNH
                     ASLAFWCGNNEIDEGLDHWGWDKEYSAEINKEWREGYTKTFHQLIPELVEKYDGTRSY
                     IHGSPYNSNWGNKETFSSSDVHDWGLWHGRMPFEAMADRLPRFASEFGFQSFPEMKTI
                     RTFATEKDFDINSDVMKIHQKSGVGNAAIKQYMDMYYHAPKNFEDLVYVGLVMQGNGM
                     KEAVEANRRGRPYCMGALYWQLNDDWPVVSWSSVDYYNNWKAQHYKMRDVFAPIALGL
                     MVKDNQLSYMTMSDKLEDVSNLTLNVQVIDFSGKKLKSFTENVTAKANSSITVKSFEV
                     DKLLTEEQKHNSVIHAWLSDKTGKTVSVKDYYFFWPNKLNLPKTSVSKSVKYEDGKYT
                     VTLSSPKLAKDVFVEIPVQGARFSDNFFDLLPGQKKVITITAPELKKSDKT"
BASE COUNT         2016 a         1689 c         1693 g         2228 t
ORIGIN      
        1 aaacggaaac cgtccacatg atattccttc gcccagtaca ctaccgattc gatcatgaat
       61 tttcgcatca tgggacgttc ggaagctgtt tcattgccgc aacccgatgc attggaccag
      121 gtactgtcct tgttgaaacg gtagaaatat cccggtgcca gcaggttgag atgcgattct
      181 tcgcccacaa aagtgtggtt atacaccaca tccatcacca cccgtatgtt agcccgatgc
      241 agcgactgaa ccatttgttt gaattctttg atacgcgcca ccggattggt cggatcggaa
      301 gagtagcttc cttcgggaac attgtagttc aacggatcgt agccccagtt gtatttattt
      361 tcgttgagtt tggtttcatc caccgaagca taatcatagg aaggcaacaa atgaacatgg
      421 gtaatgccaa gttctttcag gtgatcaatt ccggtagctt gtccggccgt gttttttgtt
      481 ccctgttcgg taaatgcgag gaatttcccc ttgtttttca ttcccgaatt gggcgacatg
      541 gagaaatcgc gcacgtgaag ttcgtaaagt gcaatatcgg caacattctt cagctcaggc
      601 cgtttatcct tttcccatcc ttcggggttt gtttcgttaa aatctataat tgcagcccgt
      661 ttaccgttga cgcccactgc ttttacccac attccgggtg tttcgttcaa ccatttttca
      721 ccggttttga tttgaaaagt gtagaacttc cctttcagat ctcccgaaac agcaaccacc
      781 caggttccgt ttttcgagcg gtgcatattt ttcatcatat aagccgctcc ttctatgcca
      841 ttgtcgaaca acagcagttt cacttcatcg gcggttggag cccatacacg gaattttgaa
      901 ccggtggctg aataggtaag ttcaaggtca ttcccgttat aaaccggata ttcgtcgaaa
      961 cttttatatt ttttgtaatt actactgcaa cctatcagta cacttgctat taccaacatt
     1021 agaatagatt ttttcatggg agtttatgtt tttttctttg ttgaaatatt ttttttcaaa
     1081 aatagcctta tttcggcaaa cttcctaata aaaacaaaaa gagtgtgaaa ttattgttca
     1141 acttcacact cttttcgttc ttcagttctg ttttatttta tgccgttgcg tttgagcaat
     1201 gcatcaatag agggttcctg tccgcggaat cgtttgtaga gtaccatcgg gtgttcggta
     1261 ccgcctttct ccagtatgtt tttacggaag gatgcggctg tctttttatc aaaaattcca
     1321 tttttctgaa actgtgaata ggcatcggca tcgagcactt cggcccattt gtagctgtag
     1381 tatcctgcag catagcctcc tgagaaaata tggctgaaag tggggctcat ggcggtattt
     1441 tcaatcgctg gaagcacctg agcaggcgtc atggcttctt tttcaaaagc gatcgcatcg
     1501 ccttcgaagg gttgctcgag agtgtgccat ttcatatcca gcaaggcaaa actcaattgt
     1561 cggagcgcca gataaccaat gttgaagtta tcggcatcct tgattttttg tatcaattca
     1621 gccggcattt tttctccggt ctggtaatgc actgcaaagt tgtcgagaaa ttctttttcc
     1681 accgcccagt tttccatgat ttgcgaggga agctcgacaa agtctcggta cacattggtg
     1741 cccgacaggg agcggtagtt ggttttggta agcatgccgt gtagtgcgtg tccgaattcg
     1801 tggagcatgg tggtcatttc gtcgaaggtg agcagtgcag gcttggtagc cgtaggacgg
     1861 gtgaaattca tgacgttgac aatatgcgga cggctgtctt tttttccttc catccactgc
     1921 cctttcatat cgttcatcca ggcaccggca cgtttgcctt cacgcggatg gtagtcggca
     1981 tagaatacgg ccacgaattt gccgtcggca tcggttacat cgtaagcttt cacctcggga
     2041 ttgtacacct ggatgcgggg attttctgta aatttaagac cgtaaagttt gttggaaagc
     2101 cagaaaatgc cctgttttac tttttcgagt tcaaagtaag gtttggtcat ttcgtcgttc
     2161 agttcatatt tttcggcttt cagtttttcg gaataatacg accagtccca gggttgaact
     2221 ttaaaatagg caccgttttt gtctgcatag gcctgaactt cggcatattc cttttcggca
     2281 gcaggtttat aggctgccag cagtttgttc agcaaatcat atacattttc cttgttttcg
     2341 gccatgcggt gaaccagtgc ataatcggca taggatttat gtccgagcag gtttgcaatt
     2401 ttcagacgca gattggcaat tttcaccaga ttttcgcggt tatcataatc gccacctacc
     2461 atacattttg atgcacttgc catatacaat tcgcgtcgca ggtcgcggtt gtcggcatat
     2521 ttcatcaccg gcacatagct ggtagctttc atattgataa gccaaccggt ttttccggct
     2581 ttctgcgcat tttctgccag catatccaac acatcagcgg gtaatccttt gagcagttcc
     2641 ttatcggtaa tgaccagttg atatttattg gtttctttca atacattttg tccgtaattg
     2701 agtgtgagca ggctcagctc tttggaaagt tcgcgataaa catctttgtc ggcatccgac
     2761 agattggctc cgttgtcggc aaaaccatca tacgtatcct taagcaacat ttgctgttcg
     2821 ggagtgagtt tcagcttatc tttttgttca tacaccgctt ttacccgtgc aaacagtttt
     2881 tcgttcagcc ggatcgaatt ggagtgttcg gtcattttag gtgagagttc ctcggcaatt
     2941 ttttgcattt cgtcggtagt ttcggcactc agcaggttgt aaaagggtga tgccactttg
     3001 gagagcaatt cccccgattt gtcgagtgca acaatggtgt tttcgaaagt cggcggcttg
     3061 gggttgttta ctatcttttc aatttccgcc ctgtgctgtt tcattgcctc ttcgaacgag
     3121 ggcatatagt actcgttttt cagttcattg aatggaattg tttgataagg agtgttgtag
     3181 ggcttgaaaa agggatttcc ggctaacgct gatccggcag cggtaattac agtcatacaa
     3241 aggattattt tctttaacat atttaattga ttttgagttt attctctgat tagtttctga
     3301 acctgtacgc cttcgggtgt taccacctgc aggatataaa tgcccggtgt gtagtcgccc
     3361 agatcgatga ctcccgagcg cttcgtctgg tttttagtca tcagtttctt accactcagg
     3421 tccgagagtg tccaccgact gacatcatgc tgttgtgtat cgaaataaaa aattcctgtg
     3481 gaaggattgg ggtaaacgtt tatttcgttg gcccggcgat gaatttctgc agatttcaca
     3541 ctggtttcgc tcacaggaat ataacttaac acaactctgg cggcctgttg tacaaccgtt
     3601 tcgctattga atctatatgg aataaaggta gtaatctgtg cgccggtttc gccgccccag
     3661 aattccagtc tggcttctat caggctgggt ttggcgtatt gaaagtcgga atagttgaca
     3721 gaagtaaccg cccgaaagtc atcatacacg ggcagacagg tcgtttttcg taacaattct
     3781 ccctgcgtat catattcata gcgatatcgc aggtcgtttt cccagtacac atatttccag
     3841 ttctgataga tttcgtcggt caatctgcct gaattatcgt aggtgtaata cacattctgt
     3901 ttgttgaccc aggtatgata tgtttcgtcg cgcaatttta gaatttctga tgatttgagc
     3961 ttcgaatttg gcttataata ggtttccgac agcgaaatat ttacccatcc ggtgtcgctt
     4021 ttttcggcat aaatctgagt ttttaccagg gaggaatcgt tgtaggttaa atttatacga
     4081 gattcaccgg ataaaaaacc ggacgagtat ttttttatag ctatcagttc gttcagtcct
     4141 gcctcattgt agctgtaatc ggtagccagc gttgttatcc agtcggcccc cacgcgcttg
     4201 tattcggttt tggaggtaag caaattctga ttgtaggtga aaactgattt cgaaataaat
     4261 tttgcaattc cgttttgcac ttcctgatgg gtttctttca ccggtttacc gtcggcatac
     4321 tcattctcaa tctgataata cgtttcccag ttaccattta tccatttact cattcgctgc
     4381 aacgtgcaca aactgcctgt ataaatccat tcggtttgtt gccggcgctg ccagtcttct
     4441 cccttccaca catatctggt ctcgagtgat ttggctttat caccgttgta atagtacaga
     4501 taacgatagg tactgtcggc cgtggtaacc tgcacttcgg taatcagcgg attggggtcg
     4561 gtttggctat aacacgctgc tcccataagc aatgcggtta tcagcaataa aagaatatgt
     4621 agtttagact gccccattta ttttttcagc aaaattcata tgtgctaacc tgcaaagata
     4681 aatacaaatg ttgatttcgg caataaacaa aatctacctt taactatttt aatattcttg
     4741 ctgatatact gatagttaga acttattgca ttttaacaat tttatcagag attaaatttt
     4801 tcagaccggc aaaaaatgat taatattgca atcaaaatta acaacctcaa acaacaatac
     4861 ggcaatgaaa aaattagtca tcccctacat cacggattta gaaactgctg atatagaatc
     4921 ttcggcagat attctggaag aaaacggaac aaaaagcgcc atcgatgtgc tcaactggcc
     4981 ggatcaatat ccttacaaac ccatcactta ttttttcacc gggcgttcgg ctgatgctgt
     5041 tttcattaaa tactccgtga aaggctccat gctgaaagcc gtttataccg aagatcattc
     5101 gccggtacac gaagacagct gtgtggaatt tttctgtatg cccgaaggag ctgataaata
     5161 caccaacttt gagttcaact gcatcggaac ctgctccgcc tcgagccgaa aaggaagaag
     5221 cgaagacgtt gtgcctttcc cggccgaaga tatggtgtca atcctgcgtt acccctcgat
     5281 gggacaccgt gcattcaaag aaatggaagg gatgttcgaa tgggaactga ccgtgaaaat
     5341 tccgctgaaa ctgatgaacc ttgatcccga taatctgcct gaaaaaatcc gggggaattt
     5401 ctataaatgt gccgatgaca cagattcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnacg cacgccgatg tatttctgaa cggagcccgc atactgcaaa ctcaaaatat
     5581 gttcgttggt caccaaatcc cggtgaaaaa ttcattgcgc gcaggggaga acagcttgtt
     5641 cattcgtttt tattctccgg taaaacgcat ggaaccggca cggctcaccg ccggatacga
     5701 atatcccgcc ggaaacgacc acagcgataa aaaagtgagc atcttcaacc ggaaagctcc
     5761 ctatcagttc ggctgggact ggggaattcg actcgttcag atgggcatct ggaaacctgt
     5821 aacactccgg ttctacaata aggccatcat tgacaatttc tatgtcaaac aaatctcaat
     5881 aacgaaagac aaagccgtaa ccgacaacca gatagagatt tactccacct ccgaaactcc
     5941 ggcgaaaata agtacgggtg ccggttttaa tagcctgcct gtactgtcgg ttgataaaga
     6001 aattgtgctg aaaaaaggaa aaaacatcgt cagcattccc ctcaccatcg acaatcctaa
     6061 cctgtggatg ccagccggat ggggcaaaca aaacctctac gatttttctg tgaaagttta
     6121 cattggtgac aagcttgttt ccgaaaaaac cgaaaagatc ggactccgca aagtcagact
     6181 ggtgcagcaa cctgatgaac acgggaaatc attttatttt gaagtaaatg atattccgct
     6241 gtttgcaaaa ggcaccaact acattcccgg cgaaattctt cgcaccaggc aggacaaagc
     6301 atactacaac cggctattcg ataatctgac cgaagccaac atgaatttta tccgcgtgtg
     6361 gggcggcggc acgtatgagg atgattattt ttatcaactg gccgatgaaa aaggaatact
     6421 tgtgtggcag gatttcatct tcggatgcgt tccctaccct tccgacgatg catttctggc
     6481 aaatgtaaaa gaagaagtca tttacaatgt gaagcgactt cgtaatcacg cttcactggc
     6541 tttctggtgt ggcaacaatg aaatagacga aggtcttgac cactggggat gggacaagga
     6601 gtattccgct gaaataaata aagaatggcg cgagggatac actaaaacat tccaccagtt
     6661 gatacccgaa cttgttgaaa aatacgacgg cacccggagc tatatccatg gatcgcccta
     6721 caactccaac tgggggaata aagaaacttt cagttcgagc gatgtgcacg actggggttt
     6781 gtggcacggc cggatgcctt tcgaagccat ggccgaccga ttgccacgct ttgccagtga
     6841 attcggattt cagtcgttcc ccgaaatgaa aacaattcgc acatttgcca ccgaaaaaga
     6901 tttcgacatc aactccgatg tgatgaaaat tcaccagaaa agcggtgtag gcaatgctgc
     6961 tatcaaacaa tacatggata tgtactatca tgcaccgaaa aatttcgaag atttggtgta
     7021 tgtaggactg gtgatgcagg gcaatggtat gaaagaagct gtggaagcca accgccgcgg
     7081 ccggccttac tgcatgggag cactttactg gcagctcaac gacgactggc cggttgtttc
     7141 gtggtcgagc gtggattatt acaacaactg gaaagcgcaa cactataaaa tgcgcgatgt
     7201 gtttgcgccc attgcacttg gtttgatggt gaaagataat cagctcagct acatgaccat
     7261 gtccgacaag ctggaagatg taagcaacct cacactgaac gttcaggtga ttgatttttc
     7321 gggaaaaaag ttgaaaagtt tcactgaaaa tgtaacggca aaggcaaatt cgagtattac
     7381 agtcaaatca ttcgaagtgg acaagctgct taccgaagaa caaaagcaca acagcgtgat
     7441 tcacgcctgg ctgagcgaca aaaccggaaa aaccgtttcg gtaaaagatt actatttctt
     7501 ttggcccaac aaactgaatt taccgaaaac gtcagtcagt aagtcggtga aatacgaaga
     7561 cggaaaatac acggtaaccc tttcatcacc aaaactggca aaagatgtat ttgtggaaat
     7621 tcctgttcag ggagcccgtt tctcggataa tttctttgac ttgctgccgg gacaaaagaa
     7681 agtaatcaca attacagctc ctgaattgaa aaaatcagat aaaaca
//
LOCUS       DAOPZK010000090         7718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_11396_length_7718_cov_22.128409, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000090 DAOPZK010000000
VERSION     DAOPZK010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11396_length_7718_cov_22.128409"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKH68_06450"
     CDS             <1..846
                     /locus_tag="PKH68_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156906.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNX12613.1"
                     /translation="CTYCNNQSFSPGYAKPIKSVTEQIEEGIAFFHHKYEAQKYLAYF
                     QSYTNTYDKLENLTRIYEEALKNPKIAGIVVGTRPDCVSIELLDYFTELSKRYYVMIE
                     YGVESTCDDTLQLINRGHDYTSAENAIRNTAGKGLHTAAHLILGLPGESREMILSHAD
                     NVSELPLTAIKLHQLQLVKGTVMARQYAENPEMFNMMTAAEYIELVIDFLERLNPEIA
                     VERFVSQSPEQLLLAPHWGLKNFEFVHKIEKRLKERNTWQGRLFRPPTSRNKCGTGSK
                     GEPKN"
     gene            complement(912..1340)
                     /locus_tag="PKH68_06455"
     CDS             complement(912..1340)
                     /locus_tag="PKH68_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNX12614.1"
                     /translation="MLKKDLQPEFVLSLCEDSEKVMQFLADVKWETGFVCRGCGHTNF
                     CSGKTYASRRCTKCKKEESATANTIFHNCKFPLNKAFSIIYSVCFQKRKMTVGEMSEE
                     LGLNPMTCWKFRTKIKSCIREHQNNPNDDVDVMDILMVAP"
     gene            1697..2374
                     /locus_tag="PKH68_06460"
     CDS             1697..2374
                     /locus_tag="PKH68_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNX12615.1"
                     /translation="MLIENNPNIIADPNSLKLKVASINERYTNLSETTCCLSCGGAIN
                     HAGVQPGEVCLDLGSGRGNDVIRMAEEAGENGFAYGIDLSDGMVKKARTNLDKFGVTN
                     AEIIQSEMESLPLNDNSVNVTISNCTINHSSNKEAVWSEVFRVLKPGGRFVVSDIYAT
                     APIADEYRNDPQAVAECWAGAVTRAEYLTMLEETGFTNIKILEESEPYAKGMAEVASF
                     TVYGEKE"
     gene            2797..4152
                     /locus_tag="PKH68_06465"
     CDS             2797..4152
                     /locus_tag="PKH68_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX12616.1"
                     /translation="MSTIQNSPLEPVPINREVRGHSKHNIIVPIKDSEDFFIVNPLHG
                     SADIISASELDMLQNQHDTDGVFAERKYLIDEAEENKAFKMAYLDFLDTRDEDEIQLF
                     FVPNYSCNFACSYCYQEGYSPAESKMTTEVVDAFFDYISREFAGRKKYITVFGGEPLL
                     PGENQRKIIEYLLNKSNEAGLDVAFVTNGYTLASYLELLKTASIREVQVTLDGTKDIH
                     DKRRYMKGGQSTFDKIIEGIDRCLQSDISINLRMVADKENLGNLPELARFAIEKGWTK
                     SPLFKTQIGRNYELHVCNSSPDKLFTRAELYNRIYELMKVHPEIIEFYKPGFSIAKYI
                     AENEELPSPLFDSCPACKTEWAFDFTGTIYSCTATVGKQGEELGTFYPTVTRNEEVIS
                     QWQDRDITTIEKCKTCDVALACGGGCASVAKNKNGSINSPDCRPVKELLSLGAAAYLH
                     P"
     gene            4358..5629
                     /locus_tag="PKH68_06470"
     CDS             4358..5629
                     /locus_tag="PKH68_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX12617.1"
                     /translation="MKEINASDFQKEVLEGGKVVVDFYSTECPPCEALAPKFEAMAEL
                     YGEKIKFLKIFRQGNRELATELGVSSSPTLLFFDNGNQILDTISGGIKKSEIQERLNK
                     MISPEEVDEIKSHQKHTVSEYDVAILGGGPAGLTAGMYLGQAKLKTVLIDPALPGGYM
                     GITHQVSNYPGYVEPQPGFMLAHYMSEQAKQTGIDFKVAVDVTSVDLAKKEIVIDETE
                     TIRAKKIIIATGTSPNTMNAPGENELKGKGISYCATCDAKYFVDKDVTVIGGGNSAVE
                     EAQFITKFARKVTMVHQFDKLTANQTAIDNLQANEKISVIYEHEPRKFEKRGDKMITT
                     IENLKTKKLSELEADGVFVFIGFKANTAVLGNTLPALNDWGYILVDEDKKTSLTDVYA
                     VGDIISKKYRQITTAVADGTIAAMSISKENP"
     gene            5664..6047
                     /locus_tag="PKH68_06475"
     CDS             5664..6047
                     /locus_tag="PKH68_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNX12618.1"
                     /translation="MKDEKNMFYGAQPIIFELAKQLRDRQTEAEIYLWDHLKFLNIPN
                     IRIKRQHPVLYFIADFYCHKAKLIIEIDGGYHNIPEQYLYDINRDEELKSLGLKVIRL
                     TNDEVLNDIQNSIKIIENEIIERLH"
     gene            6078..6518
                     /locus_tag="PKH68_06480"
     CDS             6078..6518
                     /locus_tag="PKH68_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HNX12619.1"
                     /translation="MIENYYQYNSHLASGAQPSEAQFADLKEAGFEAVVNLSPVSTRN
                     AMTAEAAVVDNTGMTYVHFPVDCSHLQPHHYATFEGIMNGIKDKKIFVHCGGNIKSSC
                     LIHMYHVLANGMDEATSLETLLKIHQPEEKWFDYFRLMGMEGVK"
     gene            6730..7185
                     /locus_tag="PKH68_06485"
     CDS             6730..7185
                     /locus_tag="PKH68_06485"
                     /inference="COORDINATES: protein motif:HMM:NF020475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HNX12620.1"
                     /translation="MKNLYSKDDLKLMRAAEDSLLMGKNRVAELMKYAGNTGVKRIGI
                     AHCVGMTREAMELKSRLEEKFEVYTVDCKYGKIHASEMLDDETARGTSCNPAGQADFL
                     KENNTDLNISFGLCMGHDIIFNMKSKAPTTTLIVKDREHKHNPYNEFKK"
     gene            7192..7611
                     /gene="trxA"
                     /locus_tag="PKH68_06490"
     CDS             7192..7611
                     /gene="trxA"
                     /locus_tag="PKH68_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNX12621.1"
                     /translation="MTTVLIIVAVVILLLAALVVYSRYKLKNMPKVEDHEKIITLTDK
                     NFNQQIKGKVVLVDFWATWCAPCRLMAPALNDLASELTDNKYRVGKVDVDANRGLAQK
                     YNIRSIPTSVIFKDGQEVARIVGVKPKATLLKEMQRF"
BASE COUNT         2520 a         1556 c         1658 g         1984 t
ORIGIN      
        1 tgcacgtatt gcaacaatca gagcttcagt ccgggatacg ctaaaccgat aaaatcagtc
       61 acagagcaaa ttgaagaagg tatcgctttt tttcatcata aatacgaagc gcaaaaatac
      121 ctggcctatt tccaaagcta caccaacacc tacgacaaac tggaaaatct gacacggatt
      181 tatgaggaag ccctgaaaaa tccgaaaata gcaggcattg tggtgggcac acggcccgat
      241 tgtgtgagca ttgaattgct ggattatttt acagaactaa gcaaacggta ctacgtgatg
      301 atagagtacg gtgtggaaag tacctgcgat gatacacttc agcttatcaa tcgcggacac
      361 gattatacca gcgccgaaaa tgctatccgg aataccgcag ggaaaggact tcatacagct
      421 gcacacctta ttctgggtct gccgggtgag agccgggaaa tgatactttc gcacgccgac
      481 aacgtgtcag agctgccact gacagccatc aaactgcatc agcttcaact ggtgaaaggc
      541 accgtgatgg cccgtcagta tgccgaaaat cccgaaatgt tcaacatgat gacagccgct
      601 gaatacattg agttggtgat tgattttctg gaaagattaa atccggaaat agcggtagag
      661 cgatttgtat cgcagtcgcc cgaacagttg cttttagctc ctcactgggg attaaagaac
      721 tttgaattcg tacataaaat tgaaaaaagg ctgaaggaaa gaaatacgtg gcagggaaga
      781 ctgtttcggc ccccaacctc ccgaaacaag tgcgggacag gctctaaagg ggagccaaag
      841 aattagaata gccatcaata ttatagtatg aacaatacta agatataaat agaaattccc
      901 ccttcagggg gttagggggc aaccatcaaa atatccatca catctacatc atcgtttgga
      961 ttgttttggt gctcgcgaat acaactttta attttggtgc ggaacttcca gcaagtcata
     1021 ggattaagtc cgagttcttc agacatttcg cccaccgtca tttttctttt ttggaaacaa
     1081 acggaataga tgattgaaaa agccttgttg agaggaaatt tgcagttgtg aaaaatagta
     1141 ttggctgttg cagactcttc ttttttgcac ttcgtacagc gccgggatgc ataggttttc
     1201 ccactgcaga agttggtatg accacatcca cgacatacaa aacccgtttc ccactttaca
     1261 tcggccagaa actgcataac tttttcacta tcttcacaca ggcttagaac aaattcgggc
     1321 tggaggtctt ttttaagcat ggttgcagcg ttgtattttt aatttgatta ttaacaatca
     1381 gaataataga atagtttttt cagtctgcaa atttagtgat tttaatcgtc attcccaaaa
     1441 taaatccttc agactttata tttcatgttt cagattttag tctaattata tttttgaaca
     1501 aaatcacact tacaatatct tgaactttcg aaaaatacag ttttttttcc ataactttat
     1561 accctcaaaa agaattacat tcaataaaaa aatattaaaa aatcagtgat atctttgtaa
     1621 tatgaaagat tgttcatata tttgcaaaaa atttcaaaca gtacattaaa acagcattta
     1681 aacaatttaa aacaagatgt tgattgaaaa taatcccaat ataatcgctg atccaaattc
     1741 actgaaacta aaggttgcat ccatcaatga gcgatacact aatctgagtg aaaccacttg
     1801 ctgtttgtcg tgcggcggtg ccatcaatca tgccggcgtg cagcccggtg aagtttgtct
     1861 ggacctgggt agcggccgtg gcaacgatgt aatacgcatg gcggaagaag ccggtgaaaa
     1921 tggttttgct tacggcatcg acctttcgga cggaatggta aaaaaagcgc gtacaaatct
     1981 ggataaattc ggggtaacga acgccgaaat cattcagtcg gaaatggaaa gtctgccgtt
     2041 gaatgacaat tcggtgaacg taaccatttc caactgcacc atcaatcaca gttccaacaa
     2101 ggaagcggtg tggagtgagg ttttcagggt gttgaaaccg ggcggacgat ttgttgtgag
     2161 cgatatttat gctactgcgc ccattgccga cgaataccgc aacgatccac aggctgtggc
     2221 cgaatgttgg gcaggtgccg taacgcgtgc cgaatacctg actatgctcg aagaaacagg
     2281 ttttacgaac ataaaaattc tggaagaaag tgaaccctat gccaagggaa tggccgaagt
     2341 ggcgagtttt acggtgtacg gggagaaaga atagaactga cttgccccct aaccccctaa
     2401 aggggggaaa aatataggta ggccaaactc atttataaca agaaatttta agaaataaat
     2461 ttttctttta ttttaatatc aattttttcc ctcagacatt tcccctttag gggattaggg
     2521 ggctttttaa tcaggctata ttaatctata ataaattcaa aaagtatgaa aagtgtagta
     2581 aaaaagggtt gtggatgcaa aaccggcaaa gaacagaaac aagcacaatg ctgtgccaaa
     2641 gtttcgaaat tcgtagccgg ctgccacgac taacccctag cccctaaagg ggtataagat
     2701 actcctttag gggctttttt gtaatagttt aatcgaaaat gtacttcatg tctttgaaaa
     2761 agaaataata agtagaataa cgtaaaattc atttaagtga gcacaatcca aaattctcct
     2821 ttagagcctg tcccgattaa tcgggaggtt aggggtcatt ccaaacacaa tataattgtt
     2881 ccgataaaag acagtgaaga ttttttcatt gtcaatccgc ttcacggaag tgccgatatc
     2941 atatcggcat ctgagcttga catgcttcaa aatcagcacg atacggatgg tgtttttgcc
     3001 gaaagaaaat acctgattga tgaagctgag gaaaataagg ctttcaaaat ggcttatctg
     3061 gattttctgg acaccaggga tgaagatgaa attcagctgt tttttgtacc gaactattcc
     3121 tgcaattttg cctgttcata ctgttatcag gagggttaca gccccgccga aagcaagatg
     3181 accaccgaag tggtggatgc atttttcgat tatatcagtc gcgaatttgc cggccgaaag
     3241 aaatatatca ccgtttttgg cggtgagccg ctgctcccgg gcgaaaatca gcgaaaaatc
     3301 attgaatatc tgttgaataa aagcaacgaa gccggattgg atgtggcttt cgttaccaac
     3361 ggatacacac tggcctctta cttggaactg ctgaaaacgg cttctatccg cgaagtgcag
     3421 gtaacactcg acggcaccaa agatatacac gacaagcgcc ggtacatgaa aggcggccag
     3481 tccaccttcg acaaaatcat cgaaggaatt gaccgctgcc ttcaaagcga tatctccatc
     3541 aacctgcgca tggtggctga taaggaaaat ctgggcaatc tgcccgaact ggcacgtttt
     3601 gccatcgaaa aaggatggac aaaatcaccg ctgttcaaaa cacagattgg ccgcaattac
     3661 gaacttcatg tgtgcaacag ttcgcccgat aaactattta ccagagccga actctacaac
     3721 cggatatatg aactgatgaa agttcacccc gaaattattg aattttacaa accgggattt
     3781 tccatagcca aatacattgc cgaaaacgaa gaattgcctt cgccgctatt cgacagctgc
     3841 cccgcctgca aaactgaatg ggcttttgac tttacgggaa ccatttattc ctgtaccgca
     3901 actgtgggaa aacagggcga agaactgggc accttctacc ccactgttac ccgaaatgaa
     3961 gaggttataa gccagtggca ggaccgggac atcaccacca tagaaaaatg caaaacctgc
     4021 gacgtggctc tggcttgcgg tggaggatgc gcttcggtgg caaaaaataa aaacggttcg
     4081 ataaattcgc ctgattgccg gccggtaaaa gaattgctaa gtctgggagc agcagcatat
     4141 ttacacccct aacctcccga ttagtcggga caggctctaa aggagaactt gcacaagcta
     4201 tttgcatcaa caacaaaata aacatatttt aatatatgaa caaatgaaca tatatcaaat
     4261 tcgtttgttt aataaataaa aaaataatca aatcgaaaat aactaattaa ttgctccgca
     4321 ttattcccct ttaggggtta ggggttgttt tacatttatg aaagaaataa acgcatccga
     4381 ttttcaaaaa gaagtactcg aaggaggaaa agtagttgtt gacttctatt caacagaatg
     4441 tccgccctgt gaggcactgg caccaaaatt cgaagccatg gccgaacttt atggcgaaaa
     4501 aattaaattc ctgaagattt tccgtcaggg taatcgcgaa ctggctacag aacttggtgt
     4561 aagttcctcg cctacccttt tgtttttcga caatggcaat cagatactgg ataccatcag
     4621 cggcggcatc aaaaagtcgg aaattcagga acgactgaac aaaatgatta gtccggagga
     4681 agtggatgaa atcaaatcgc atcagaaaca tacggtttcg gaatatgatg tggctattct
     4741 gggaggcggt cctgccggcc ttacagccgg tatgtatctc ggtcaggcca agctcaaaac
     4801 cgtactgata gatcctgcac tgcctggcgg atacatgggc attacgcatc aggtatccaa
     4861 ctatccgggt tacgtggagc cgcaaccggg atttatgctg gctcactaca tgagcgaaca
     4921 ggccaaacaa accggtatcg atttcaaagt ggccgtagat gtaacttcgg tagatctggc
     4981 aaaaaaagaa attgtgattg acgaaacaga aaccatccgg gctaaaaaaa tcataattgc
     5041 caccggcact tcgcccaaca cgatgaatgc accgggtgaa aacgaactga aaggaaaagg
     5101 aatatcctac tgcgctacct gcgatgccaa atactttgta gataaggacg ttaccgttat
     5161 cggaggcgga aattcggctg ttgaagaagc acagtttatc accaaatttg cccgcaaagt
     5221 aaccatggtt catcagttcg ataagcttac ggctaatcag acagccattg ataatctgca
     5281 ggcaaacgaa aaaatcagtg tgatatatga acatgaaccc cgcaagttcg aaaaacgagg
     5341 cgataaaatg atcactacca tcgaaaatct gaaaacaaaa aaactttcag aacttgaagc
     5401 cgacggtgtg ttcgttttta tcggatttaa agccaataca gcggttcttg gaaatacgct
     5461 gcccgccctc aacgactggg gctacatcct ggtggatgaa gacaagaaaa ccagcctgac
     5521 cgatgtgtat gccgtgggcg atatcatcag caaaaagtac cgccagataa ccaccgctgt
     5581 ggccgacgga accattgctg ccatgtcaat aagtaaagag aacccctaac ccctaaaggg
     5641 gaaaaagata gtccaactac aggatgaaag atgaaaaaaa tatgttttat ggagctcaac
     5701 ctattatttt tgaattagca aaacaactta gagacagaca aacagaagct gagatttatt
     5761 tgtgggatca tttaaaattt ttgaatatac caaatatcag aataaagcgc cagcatcctg
     5821 ttttatattt cattgctgat ttttattgcc ataaagcaaa attaataata gaaattgatg
     5881 gtggttacca taatattcct gaacaatatt tatatgacat aaatcgagat gaagaattaa
     5941 aaagtctggg attaaaagtg attagattaa caaatgacga agttttaaat gatattcaaa
     6001 actcaataaa aataatagaa aatgaaatca ttgaaagatt acactaaaac attccccttt
     6061 aggggttagg ggtacttatg atcgaaaatt attatcaata caacagtcat cttgcttcgg
     6121 gagctcagcc ttccgaagct cagtttgccg atttgaaaga agccggattt gaagccgtag
     6181 tcaatctctc gccggtaagc acccgcaatg caatgaccgc tgaagctgcc gtagttgaca
     6241 atacaggcat gacatacgtt cattttccgg tagattgttc acatttgcaa ccgcatcatt
     6301 atgctacatt tgagggaata atgaatggaa taaaggataa aaaaatattt gttcattgcg
     6361 gtggaaacat caagtcatcc tgcctgatac acatgtatca tgtgctggcg aacggcatgg
     6421 atgaagcgac ttcgctggaa acgctgctta aaattcatca acctgaagaa aaatggttcg
     6481 actatttcag actgatggga atggagggag ttaagtagtt tgcagtttct agtttttagc
     6541 attacagatt ggaaatacca atcagtagtt taatacagtc ccgaaattct cccgaaataa
     6601 attcgggaca ggctctgaag gggatttcaa gagtagtttg tgaattaaaa ataataaatt
     6661 taattaagag gcgaaagtaa ttacacatcc cctttagggg cttggggtgg cattttattt
     6721 aaaataaaaa tgaagaattt atattcaaaa gatgatctca aattgatgcg ggcagcagaa
     6781 gactcgctgc tgatgggtaa gaaccgcgta gctgagctga tgaaatatgc cgggaataca
     6841 ggtgtgaaac gcatcgggat agcacactgc gtaggtatga cccgcgaagc tatggagctg
     6901 aaaagtcgcc ttgaagagaa atttgaggtg tacacggtgg attgcaaata cggaaaaata
     6961 catgcttctg aaatgctcga cgatgagacg gctcgaggca catcctgcaa tccggccggt
     7021 caggctgatt ttctcaaaga aaataacacc gatctgaata tatcattcgg gttgtgtatg
     7081 ggacatgata tcattttcaa catgaaatct aaagccccta ccactaccct catagtaaaa
     7141 gaccgggaac ataaacataa tccatataac gaattcaaaa aataaaccac tatgacaaca
     7201 gtattaataa ttgtagccgt tgtaattctc cttttggccg cattggtagt gtattcaaga
     7261 tataaactga agaatatgcc gaaagtagag gatcacgaaa aaatcattac ccttaccgat
     7321 aagaatttta atcaacaaat aaaaggaaaa gttgtactgg tagatttctg ggctacctgg
     7381 tgtgctcctt gtcggctgat ggctcccgca ctaaatgatc tggcctcgga gcttaccgac
     7441 aacaaatacc gggttggaaa agtagatgtg gatgccaacc gtgggctggc tcaaaaatac
     7501 aatatccgca gtattccaac atctgttatt tttaaggacg gacaagaagt tgcacgtatt
     7561 gtgggtgtaa aacccaaagc cactttgctt aaagagatgc aaagattcta agccaagcac
     7621 ttctatcaag gaaatttccg acttttttca aaaattattt ggagcaacta aaaaaaattc
     7681 ttacatttgc acctcaattg caaaagacct tatcccga
//
LOCUS       DAOPZK010000091         7627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_11599_length_7627_cov_20.361067, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000091 DAOPZK010000000
VERSION     DAOPZK010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11599_length_7627_cov_20.361067"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKH68_06495"
     CDS             complement(<1..557)
                     /locus_tag="PKH68_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12622.1"
                     /translation="MKTLIKLSGLLLFLLVGCTLDQGMDELNAPALTKSKDQVELQVQ
                     EPKTVTVPWKCEFASDPDRSLSPLQCNPLPANMFIGGGGWVRGNATHLGKVNQAESHY
                     INTGCDLNPVLMQLTGWVEGKITGANGDYFYYSGFAVTQLPEGTFEGDIVMDDGTGKF
                     EGCKGAVHMIGSVDFTTGIMTWKGEG"
     gene            complement(874..3048)
                     /locus_tag="PKH68_06500"
     CDS             complement(874..3048)
                     /locus_tag="PKH68_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX12623.1"
                     /translation="MKKILQSIDEWQTRKLTFPGIDDNTLTIRKNIWLSSFASTFSIA
                     GMTLLALLLDIPTIVEYGLVLIALSLPVLFGLPFTKRNYHWLLFPVQLSIILVTCYFM
                     IRLGGLLHSGGLLVTGFSILFMSINMQNPRLTLGLFIVYILTLIATVILETVFSPTHE
                     LTDFQNKLFFIVNLTWQAGYTLIIIINNINQKKQLWELKQAEATRLKELDETKTKLLT
                     NISHEFRTPLTIILGMAKLVREKPEEWLKKGTEKIIQSGYALLHLVNDLLDMARLEAG
                     AMTVNCLQQDIITQLRYLVGSFSSVALEKNIDLKYSPHIGEFLMDFDADKLMQIVSNL
                     LSNALKFTPNNGKVELATENSLNPHTFSIRVKDNGSGIAAEHLPQIFDRFYRVENPLL
                     QSQSGSGLGLALTRELIRLMNGTITVESEPGVGTLFTITLPVTNEAPVKEQMRETETS
                     KDEISDTVGVTSSEKSPEIKPSDENAPVVLIVEDNADLTEYLQHILAAEYQVISASDG
                     EAGLNRAVEMIPDIIVSDVMMPVMDGIAMLGQLKNDIRTSHIPVVMLTAKADIASRLT
                     GLEKGADDYLAKPFHEKELRIRLRNLIDMRERMQLHFASLTPDEGHLKDKSLLIENEF
                     ISKIRAFMEINIAAEEFDIHLLCQETAMSRAQLYRKFKALTGKSVFEYLRTLRLHKAK
                     ELLLSSTLNVTQVCFDVGFNNLSHFSRIFTEEFGRKPSDFRK"
     gene            3534..4418
                     /locus_tag="PKH68_06505"
     CDS             3534..4418
                     /locus_tag="PKH68_06505"
                     /inference="COORDINATES: protein motif:HMM:NF023085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HNX12624.1"
                     /translation="MKTLKSVLDEDFSKKTKNELIELLTALESALTPQVYERISGILP
                     SELQGLTKKEIVDIAESFKAGYDPGWESSVAKLSSDVMKLIFGQMAADEKGFRVAGAS
                     DSDFAAELGSIDFATIIGGPLDACVKAQSNASISTVSFINEVGFETDTTSGTKKLRMA
                     EFMYKKNVPNPEFDSTKPVSDTNKKTIEQDAEIKVPFIALLNVPSFRIESCEVDFNVK
                     LNSTYTKNVSSEFGISAGASGGIGPVKFKVDVSYKRSSSTGIKVEKEYSLAVKVRATN
                     DEMPAGLEKVLGMLGSTN"
     gene            4423..5121
                     /locus_tag="PKH68_06510"
     CDS             4423..5121
                     /locus_tag="PKH68_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12625.1"
                     /translation="MIKLSDYLDYLNNEIIQARKKADENTVLVAKEYAKHEYLKFFKA
                     PRYAFPSVKMDIPLKITDISSRSKYNFDLNEEKFLNDINEKIKTVNQEKRLNLAPLNK
                     EQLQKPEFQQLFKTLESKDQKMVKNLSDELNKIDLLPQIKSLKVGVFRPQDTKTETET
                     AEMKKILRDVISNNFSLVSTKLNDLFIDPKTSGAVDKDKLFINLHIEMEEEGIRLVTF
                     TDKNGKTVEEIIFE"
     gene            5122..5706
                     /locus_tag="PKH68_06515"
     CDS             5122..5706
                     /locus_tag="PKH68_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12626.1"
                     /translation="MQEFIHNKVKVLDELFTEFNQVQRLYAEKSFEFESRFTVFLNKL
                     SDYFKTSGDQAKESEVLRVTNMLHTVKRGFNPTNQEKVNTGRRELLWGFAYNGIENLN
                     SLMQEIYGKETSKLEEGEELLSNLILNLIQQGFLTDQKLMELDTIPKIEAYWNSLLTQ
                     NGSITVIHKKLLMKLISEDIYLLLEKIIAKIKSQ"
     gene            5717..6976
                     /locus_tag="PKH68_06520"
     CDS             5717..6976
                     /locus_tag="PKH68_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNX12627.1"
                     /translation="MKQNRYIVLLKDQHKSSIQSVEREFSVSVTSSELLSKDNRSYNI
                     IDSKNAVLYKNLGVVVVDDMDEQQLTLSVKDTKSPVIWFEKERDFFAADEITLINELK
                     TTVDQLKSKITELENFILHKPIPQQPVIEWQWGLKAIGMDKTLFTGKGIDVCILDTGF
                     DISHPDFTGRTIEGKSFIQGEDWDKDPNGHGTHCAGVACGNVRNDTGVRYGIAKDCNL
                     KIGKVLSNSGNGTTSSIIDAIDWAITKKFRIISMSLASPVQINEKPSVLFETVGTRAL
                     ENNVLIIAAAGNDSQRPSLPAPVSAPANSLSIMAVAAIDNQMKVARFSNAGINASTGG
                     NINVCAPGVDILSSYPRKSGSTTDYKLLSGTSMATPHVSGLAALYMEQFPDLTARQVW
                     ELLETKAKPIENLPYRDIGKGLIQTIQ"
     gene            6987..7193
                     /locus_tag="PKH68_06525"
     CDS             6987..7193
                     /locus_tag="PKH68_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12628.1"
                     /translation="MIIYFGVLKDGINYVKIKKKLTEAGLIIRNYYPALKIVKFESEK
                     KITQADFEFFVAIEEEKNDFSIQM"
     gene            7370..>7627
                     /locus_tag="PKH68_06530"
     CDS             7370..>7627
                     /locus_tag="PKH68_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12629.1"
                     /translation="MKKIFLLPMIVVAVLAACTQVPTVKPLDENAAKAELMITMDSID
                     LAFKNKNLDAFMAFFDEKCMFYGTDPAEKWDKKTFQAEMQKM"
BASE COUNT         2360 a         1508 c         1619 g         2140 t
ORIGIN      
        1 ccttcgcctt tccaggtcat tatgcctgta gtaaaatcaa ccgaacctat catatgaact
       61 gctcctttac atccctcgaa ttttccggtt ccatcatcca tcactatgtc accttcaaat
      121 gttccttcgg gcaactgagt tactgcaaat ccggaatagt agaagtaatc gccgttggca
      181 cccgtgatct ttccttctac ccagccagtc aattgcatga gcacgggatt taagtcacaa
      241 cctgtattta tatagtgact ttcagcctga tttactttgc ctaagtgagt ggcatttcct
      301 cttacccatc cgccgcctcc tataaacatg ttagcgggta gtgggttgca ttgaagaggt
      361 gaaagagacc tgtccggatc ggacgcaaat tcacatttcc agggtacggt caccgtttta
      421 ggttcctgaa cctgcagttc tacctgatct ttcgattttg ttaatgccgg agcattcagt
      481 tcatccattc cctgatccag ggtacatcca accagtaaaa ataaaagtaa tcctgagagt
      541 ttaattaaag ttttcatcgt ctgatcttgt taagaagaaa gaaaaaattt agtaaaatat
      601 cttttttgca atgtccggcg gtttcctaat ttccaaatag gagtgtcatt ggttgttaat
      661 tagcttactt agccggtttt tctcaggtta aaattctgta ttcaaacaga ttagtcgctg
      721 ttgtctttgg agaaatatga atacatttca atatttccgg gaaccacgtt catacacttt
      781 ttttacaaaa caaatttatc atctgtttgc agcaaaaggt tttcttaccg tctcaacttg
      841 ttttctgaca gtatcaaagt gtcaaaagag gaattatttt ctgaaatcgc tgggtttacg
      901 tccgaattct tcggtgaaaa tgcggctgaa gtgggagaga ttgttaaatc cgacatcgaa
      961 acagacctgc gttacgttga gcgtggagga gagcagcagc tcctttgctt tgtggaggcg
     1021 gagtgtgcgc aggtattcga acaccgattt gccggtcagt gccttgaatt tgcggtaaag
     1081 ctgggcgcgg ctcatggctg tttcctggca caaaagatga atgtcaaatt cttcggcggc
     1141 tatatttatt tccatgaatg ctcttatttt gctgataaat tcattttcaa tcagcagcga
     1201 tttatccttc aaatgtcctt catcgggtgt cagcgaggcg aaatgcagtt gcattcgctc
     1261 acgcatgtct atcagatttc ggagcctaat gcgtagctct ttttcatgaa acggttttgc
     1321 caggtaatca tcagcgcctt tctccagtcc ggtgagacgc gatgcgatgt cggctttggc
     1381 ggtgagcatg accaccggaa tgtggctggt acggatgtcg tttttcaatt gtccgagcat
     1441 tgctatgcca tccatcacag gcatcatcac atcactgact atgatatccg gtatcatttc
     1501 tacagcccta ttcagtcccg cttcaccatc ggaagcactt atcacctgat attccgcagc
     1561 cagaatatgt tgcaggtatt cggtcagatc ggcgttatct tccacaatga gcactaccgg
     1621 agcattttcg tctgaaggtt ttatttcagg tgacttctca ctacttgtaa ccccgacggt
     1681 atctgaaatt tcatcttttg aggtttcggt ttctctcatt tgctccttga caggtgcctc
     1741 gttggtcaca ggaagcgtga ttgtaaacag agttcccact cccggttcac tctccaccgt
     1801 aatggtaccg ttcatcagtc tgatcagttc gcgcgtcagt gccagtccca atcccgagcc
     1861 gctttgcgac tgaagcagcg gattttcaac gcggtaaaac cggtcgaaaa tctgtggcag
     1921 atgttcggcc gcaattcccg aaccgttgtc tttcacacgg attgaaaacg tatgtgggtt
     1981 tagggagttt tcggtcgcca gttccacttt tccattattt ggagtaaatt tcagtgcgtt
     2041 ggagagcagg ttggagacaa tctgcatcaa tttatccgca tcaaaatcca tcaaaaactc
     2101 cccaatatga ggcgaatact tcaggtcgat gtttttttcc agtgccacag aactgaacga
     2161 ccccacaaga taccgcaact gagtgataat gtcctgctgc aggcaattta cggtcatggc
     2221 accggcttcg agcctcgcca tatcgagcag gtcgttgacc agatgcagca acgcataccc
     2281 gctttgaata attttttcag tccctttttt gagccattct tccggttttt ccctgaccaa
     2341 cttggccatg cccagaataa ttgtaagggg tgtccgaaac tcgtgtgaga tattggtgag
     2401 cagttttgtt ttggtttcat ccagttcttt cagtcgggtg gcttcggctt gcttcagttc
     2461 ccagagctgc tttttctgat tgatgttgtt gataatgata atgagtgtgt aaccggcctg
     2521 ccaggtaaga ttgacaataa aaaacaattt gttttggaaa tcggtcaact cgtgtgttgg
     2581 tgaaaatacg gtttctaaaa tcacggtggc aatcaacgtg agaatataga ctataaacaa
     2641 acccagggtt aaccgcgggt tttgcatatt gatggacatg aacaggatag aaaaacccgt
     2701 gacgagaagt cctccggaat gaagcagtcc tccgagccgg atcatgaagt aacatgtgac
     2761 cagaataatc gataactgca cgggaaacag cagccagtgg taatttcgct ttgtaaaagg
     2821 taatccaaac agaacgggaa gtgataatgc gatgagcacc agtccgtatt ccacaatggt
     2881 aggtatgtcc agcaacagtg caagcaacgt catacccgct atggagaaag tggaagcaaa
     2941 ggaacttagc cagatatttt tcctgatagt gagggtgttg tcgtcaatcc ccggaaaagt
     3001 taatttccgg gtttgccact catcgattga ttgcagaatt tttttcattt tttcaatttc
     3061 caattgtctg taagttgacg atggaagaaa gcggataatg agctgaattt gagaatgatt
     3121 tatctgaaac cacacgccgg aaaaaatctg gtttatgtat attctaattc attcatatac
     3181 aatgtaaaaa cactttttat gccgtaatag ttttttaaaa ataataatac taaatcacct
     3241 gctgtaagtt attgctggtt cgggtaccat tggcaatgca ccaacatcat tcttttgttt
     3301 attacgaatc ggttttacaa caaattgtta catatttatg cgaaaactat aaatataata
     3361 gataaattat gttttattaa tcaaaatgca atcaaaatcg tattgaatta tacaaaatat
     3421 aacattgttg acgaggaata attgtttata atttgttata aaagtattat tgtcgtttta
     3481 tgtgttaata aattcatttt caggtacttt aatctaaaaa aaaagcatgc attatgaaaa
     3541 ctttgaaatc tgttctggac gaagacttca gtaaaaaaac aaaaaatgaa ttgattgaat
     3601 tgttgacagc attggaaagc gcattaacac cacaggtgta cgaacgaatt agcgggattt
     3661 taccttcaga actacaaggt ctaaccaaaa aagagattgt tgatattgca gaaagtttca
     3721 aagcgggtta tgatcccggc tgggaaagtt cggtagcaaa actatcatcc gatgtaatga
     3781 aattgatttt tggtcagatg gctgcggatg aaaagggatt tagagttgca ggtgcatcgg
     3841 attcggattt tgcagcagaa ttgggtagta ttgactttgc caccattatc ggcggaccac
     3901 tcgacgcttg cgtgaaagcg cagtccaatg cttccatcag cacggtaagc tttatcaatg
     3961 aagtaggatt tgaaacagac acaacttccg gtaccaagaa actcagaatg gccgagttta
     4021 tgtacaaaaa aaatgttcca aatccggaat ttgatagtac gaaacctgtg agtgatacaa
     4081 ataaaaagac aatagaacag gatgccgaga tcaaggtgcc attcatagct cttctcaatg
     4141 ttccgagttt cagaatagaa tcctgcgaag tggatttcaa tgtaaaactg aactccacct
     4201 acaccaaaaa tgtaagcagt gaattcggaa tcagtgcagg agcgtcgggt ggtatcggtc
     4261 cggtaaaatt caaagtggat gtttcgtaca aacggtcttc gagcaccgga attaaagtgg
     4321 aaaaggaata ttcactggct gtgaaagtga gggcaaccaa cgacgaaatg ccagccggtc
     4381 tggaaaaggt tttaggtatg ttaggatcaa caaactaacc tcatgattaa actttctgat
     4441 tatctggatt atctcaacaa tgagataatc caggctcgga aaaaagccga cgaaaatacg
     4501 gttcttgttg ccaaagaata tgcaaagcat gagtatttga aattctttaa ggctcccaga
     4561 tatgcttttc cgagtgtgaa aatggatatc ccattaaaaa taacggatat aagttcccgg
     4621 tcaaaatata attttgatct caacgaggag aagtttctga acgatataaa tgaaaaaatc
     4681 aaaaccgtta atcaggaaaa gcgcctcaat ttagcaccgc taaacaagga acaactgcaa
     4741 aaacccgaat ttcagcagct tttcaaaact ctggaaagca aagaccagaa aatggtgaaa
     4801 aacctgagcg acgaacttaa taaaatagat cttctgcctc agattaagtc gctgaaagtc
     4861 ggggttttca gacctcagga taccaagaca gaaacagaaa ccgctgagat gaaaaaaata
     4921 ttacgggatg ttatcagcaa caatttttca ctggtttcta caaaactgaa tgatctgttt
     4981 attgatccaa aaacctcggg agcagtggat aaagacaaac tattcatcaa tctccacatc
     5041 gaaatggagg aggaaggaat acggcttgta actttcaccg ataaaaatgg caagactgta
     5101 gaagaaataa tttttgaata gatgcaggaa tttattcaca acaaagtaaa agtcctagac
     5161 gagcttttta ctgaattcaa tcaggttcag agactgtatg ctgaaaagtc gtttgaattt
     5221 gaaagtcggt ttaccgtttt tctgaataaa ttgtcggatt attttaagac ctcgggcgac
     5281 caggccaaag aatcggaagt gctccgcgta accaatatgc ttcatacggt gaaaagaggt
     5341 ttcaatccca ccaatcagga aaaagtaaat accggcagaa gggaattgtt gtgggggttc
     5401 gcctacaacg ggattgaaaa tctgaactcg ctgatgcagg agatttacgg aaaagaaacc
     5461 tcaaaactcg aagaaggcga agaacttctt tccaatctga ttttgaatct tattcagcag
     5521 ggatttctga ccgatcagaa actgatggaa ctggatacaa ttccaaagat tgaagcttac
     5581 tggaattctc ttctaactca aaacggatct atcacggtaa tccataaaaa actactgatg
     5641 aaattaatat cagaagacat ttatttgctg ctcgagaaaa ttattgccaa aataaaatct
     5701 caataaacca ataattatga aacaaaacag gtatatcgtt ttattgaaag atcagcataa
     5761 atcttcaatt caaagcgtgg aaagggaatt ctctgtaagt gtaacttcat ccgaattgtt
     5821 gtcaaaggat aaccgttctt ataatattat tgacagtaag aatgctgtcc tttataaaaa
     5881 tctgggagtt gtggttgttg acgatatgga tgaacaacaa ttaaccctgt cagtcaaaga
     5941 cactaaaagt ccggttatct ggtttgaaaa agaaagagat ttttttgcag ccgatgaaat
     6001 aacgcttatc aatgaactga aaacgaccgt tgaccagttg aaatccaaaa tcaccgaact
     6061 ggaaaatttt atcctgcata aacccatacc tcagcagccc gtcatcgaat ggcaatgggg
     6121 cctgaaggcc atcggtatgg ataaaacgct attcaccggc aaaggaattg atgtttgcat
     6181 actggatacc ggatttgaca tttcacaccc cgattttaca ggaagaacta tcgaaggaaa
     6241 atcatttatt cagggtgagg attgggacaa agacccaaac ggacatggca cacactgtgc
     6301 cggagttgct tgtggaaatg taaggaatga taccggcgtc cgttatggta tagccaaaga
     6361 ttgtaacctg aaaattggaa aagtgctttc caatagtggc aatggaacca ccagcagtat
     6421 tattgatgcc attgactggg cgataaccaa aaagttccgg ataatttcca tgtcgctcgc
     6481 atcgccggta cagattaacg aaaagccctc tgtgctgttc gaaacggtag ggaccagagc
     6541 ccttgaaaac aatgtactta tcatagctgc ggccggcaac gacagtcagc ggccatctct
     6601 gccggcaccc gtatcggctc ctgccaattc gctttcaata atggctgtgg cggctattga
     6661 taatcagatg aaagttgccc ggttttccaa tgccggaatt aatgcaagca ccggtggcaa
     6721 tatcaacgtt tgcgccccgg gtgtggatat tctgagctcg tatccgcgaa aaagcggaag
     6781 tacaaccgat tataaactgc taagtggcac cagtatggct actccacatg tatcgggact
     6841 ggctgcgttg tacatggaac aatttccgga tttaaccgcc cggcaggttt gggaattgct
     6901 cgaaactaag gcaaaaccga ttgaaaattt accttacagg gatatcggta aaggattaat
     6961 ccaaactatt caataaataa agccgtatga taatctattt tggagttttg aaggacggga
     7021 taaattacgt taaaatcaaa aaaaaactta ctgaggcagg tttgattata cgcaactatt
     7081 acccggccct gaaaatcgta aaatttgaaa gtgaaaaaaa aatcacacag gctgatttcg
     7141 aattttttgt tgccatagaa gaagaaaaga acgatttctc tattcaaatg tagtttttga
     7201 agttaaatta tctattcccg aaaaaaccgt caatctatta aacgcccatg tccaactaaa
     7261 aattttggac acacaatggt attattaaaa aacgtacaac aaatgataga cgctcaatat
     7321 tcctccgtca gctgacggat tagggggccg ataaataaat tataaacaca tgaaaaagat
     7381 ttttttatta ccaatgatag tggtggcagt actggcagcc tgtactcagg ttcccactgt
     7441 caaaccgctg gatgaaaatg cagccaaagc agaacttatg ataactatgg atagcattga
     7501 tttggctttc aaaaataaaa atctggatgc tttcatggct tttttcgacg aaaaatgtat
     7561 gttctacggt accgatcctg ctgaaaaatg ggataaaaag acttttcagg cagaaatgca
     7621 gaaaatg
//
LOCUS       DAOPZK010000092         7583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_11709_length_7583_cov_21.722901, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000092 DAOPZK010000000
VERSION     DAOPZK010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11709_length_7583_cov_21.722901"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..481
                     /locus_tag="PKH68_06535"
     CDS             26..481
                     /locus_tag="PKH68_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNX12630.1"
                     /translation="MQNKEVSMSDYKGKVVLVVNTASKCGFTPQFAGLEELYKKYKDQ
                     GLVILGFPCNQFGNQDPGTDEEIQSFCQVNYGVTFPMFSKIDVNGKNAHPIYKYLKNS
                     LSGAITDDIKWNFTKFLIDKSGKPIKRYGSNVKPADLETDIQEALNAKK"
     gene            716..1996
                     /gene="metK"
                     /locus_tag="PKH68_06540"
     CDS             716..1996
                     /gene="metK"
                     /locus_tag="PKH68_06540"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNX12631.1"
                     /translation="MGYLFSSESVSEGHPDKVADQISDAIVDNFLAQDASSKVACETL
                     VTTGQVVVAGEVRSQSYVDVQSVARGVINRIGYTRSDYMFDGNSCGIFSAIHEQSADI
                     NRGVERQDPMNQGAGDQGMMFGYACNETVNYLPVALDLSHALVKELAYIRREGKVMTY
                     LRPDAKSQVTIEYNDDNQPEKVHTIVVSTQHDEFIKPTDTKTQEQADEEMLSIIYNDV
                     KNLLIPRVKAKVIQFDQLIKDDFVLYVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKG
                     AHGGGAFSGKDPSKVDRSAAYATRHIAKNLVAAGVSDEVLVQVAYAIGVAKPMNIYIN
                     TYGKSKVSLSDAQIAEKVEKLFDMRPKAIEERLKLRTPMYEETAAYGHVGRTPVTKEK
                     IFKDGNGNIFKQTVDLFTWEKLDMVNEIKKEFGL"
     gene            2182..4599
                     /locus_tag="PKH68_06545"
     CDS             2182..4599
                     /locus_tag="PKH68_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX12632.1"
                     /translation="MNRDEIRNYIIQMYETLKKWLFQFIDWRERRIVRFYGLPKWKKT
                     ANTILTFFCLFLFYLLIVDINFLWLFGKSPSLRAISNPPQSLSSEIISADNQLIGKYF
                     NENRKLVKFEDISPALINTLVSTEDRRFYKHFGVDIHGLFGAMKDMVQGNPRGASTIT
                     QQLVKNMYKTRSQYSRGLLGSMPGLRLPIEKTKEWTAALKLELFYSKKDILEMYLNTV
                     SFGSNAYGIFTASKTYFNTTPDKLSYEQSAVLVGLLKAITKYNPYLNPENSINRRNVV
                     LSQLAGNKYITPQVADSLKKIPLRLNYNIEKPNEGIAPYFRANLTRFLEKWGENNGYD
                     IYADGLKIYVTIDSRMQKYAEEAVRKQMRQLQRNFFEHWSGQNPWRDENGNELKNFIP
                     EIAKRTKVYRQLATKYANNSDSINKYMNIPRKMKVFDYDKGEKTVTMSPMDSIRYMNH
                     FLHCGFLAMEPDTKYVKAWVGDVNYDYWQYDKVAQSKRQPGSTFKLFVYTAALIAGKS
                     PCDKVEDKPFVWGTGKYAWRPKNANGGYSYADLSLKRAFAKSVNTVAAQLAKEVGIAN
                     VAKCAHLLGIKSKLEEVPTLSLGASDVTLLEMVNSYSTIAGEGMYHSPIIVTKIVDKD
                     GYVIYEEKIKTKRVISYENAFLMTELLKGGITEPGGTSRALWNYDLMRWDTEFGGKTG
                     TSSNYSDAWYIGITPKLVAGSWVGAEHRSVHFRSGSMGQGSRTALPVFALFMEKVLSD
                     NDLAQYRAKFPAEPKQTITRDYKCQSYYRTDTVAEDSIDVEPIESLPVPDLNMGTETE
                     KPAPVLP"
     gene            4671..5498
                     /locus_tag="PKH68_06550"
     CDS             4671..5498
                     /locus_tag="PKH68_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNX12633.1"
                     /translation="MNKIISKEYFSANVVKFTVDAPLIAKARRAGHFVIVRVGEKGER
                     IPLTIADADISKGTITLVVQRMGVSSTKLCALEAGDYITDLVGPLGKATHIENFGTVV
                     CACGGVGTAPMMPIVAALKKAGNKVITVIAARTKDLVILEEQMRENSDEVIVMTDDGS
                     YGNKGLVTNGVEAVIQREKVDLCVTIGPAIMMKFVSLLTKKYDIPTVASLNTIMVDGT
                     GMCGACRVTIGGKTKFVCVDGPEFDAHQVDFDEMLMRLGGYKDQEKEEMERKILHTK"
     gene            5504..6961
                     /gene="gltA"
                     /locus_tag="PKH68_06555"
     CDS             5504..6961
                     /gene="gltA"
                     /locus_tag="PKH68_06555"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNX12634.1"
                     /translation="MNTRDEQWRVDLRAKIKAKDRTNLTRVHMPELDPEYRSHIRNEE
                     VNLGLTPAQAMAEAQRCLDCVNPTCMEGCPVSIKIPDFIKFIETGEFLEAAKVLKMTS
                     ALPAVCGRVCPQERQCEEKCIHVKMKKESVAIGHLERFAADFERESGQISVPATAPAN
                     GIKIAVVGSGPAGLAFAGDMAKLGYEVVVFEALHEIGGVLKYGIPEFRLPNKIVDVEI
                     DNLKAMGVTFINNCIIGKTIAIQELEENGYKGIFVASGAGLPRFMNIKGENLVGVMSS
                     NEYLTRVNLMDAANPESDTPVYKGKKVAVIGGGNTAMDSVRTARRLGAEKAIIVYRRS
                     EEEMPARLEEVKHAKEEGIEFLTLHNPIEYIGNETGRVTQMVLQLMELGEPDASGRRS
                     PVEIPGKTRIVDVDEVIVSVGVSPNPLIKQSVPELETTKWGTLVVNQDTMQSVSLPII
                     FAGGDIVRGGATVILAMGDGRRAAKAMDEWIKNKS"
     gene            complement(7041..>7583)
                     /locus_tag="PKH68_06560"
     CDS             complement(7041..>7583)
                     /locus_tag="PKH68_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12635.1"
                     /translation="GLDVKTIKADSRLLSKSVMIYGNYTNKMFQLKAKSVLGQNLSDL
                     QMPMGYGISGYDADNKRATYTNFNQSGSWINLVYGKTWQVGIFGGYLRNLGTDKNLLP
                     SSTGDFTVYSSGYFSGNQHLNQLFRVAPHFSYNFKNIRLGLEYDLTNAEYGKLNAEGK
                     VTNPYSINNHRIVGAVVYNF"
BASE COUNT         2355 a         1496 c         1707 g         2025 t
ORIGIN      
        1 tttttacgat tttaaggcat tgaatatgca gaacaaagaa gtaagcatga gcgattataa
       61 aggaaaagtg gtattggtag tcaatactgc cagcaaatgc ggtttcacac ctcagtttgc
      121 aggcctggaa gaattgtaca aaaagtacaa agatcagggg ctggtaattt tgggctttcc
      181 atgcaatcag tttggcaatc aggaccccgg aacggatgaa gaaattcagt cattttgtca
      241 ggtgaattat ggtgtgactt tccccatgtt ttccaaaatt gatgtgaatg gcaaaaatgc
      301 tcatcctatt tataaatacc tgaaaaatag cctttcagga gcaattaccg atgatatcaa
      361 atggaatttt accaaattcc tgatagataa atcgggaaaa ccgataaaaa gatacggttc
      421 caatgtgaaa cctgctgatt tggagacaga tatacaggaa gcattgaatg caaaaaaata
      481 atatacatca aaattcattg tttgtttttt caaaaataaa aaaatatagt atctttgcag
      541 catattaaaa gtggatagat ttggactgct tgcaaccacg aaaaaaaatg ctaaaatata
      601 actcactttc catctttttt ctcccagtcc tgtataatca agtcctcata aattaagtat
      661 taatctatga ttgctatgct tagtggcata tcggtcaata aaaattattc tgattatggg
      721 atatttattt tcttccgaat cggtgtccga aggacacccc gacaaagtag ccgatcaaat
      781 ttcggatgca atagttgata attttctggc acaggatgcc agctccaaag tggcctgcga
      841 aacgctggtt accacaggtc aggtagtggt ggcgggcgaa gtgcgctcac agtcgtacgt
      901 agatgtgcag tcggtggcac gtggcgtgat caatcgcatt ggttacacca gaagtgatta
      961 tatgtttgat ggaaattcat gtggcatttt ttcagccatt catgagcaat cggctgatat
     1021 caaccgcggt gtggagcgcc aggacccgat gaaccagggc gccggtgacc agggaatgat
     1081 gttcggctat gcatgcaacg aaacggtgaa ttacctgcct gttgcgcttg atttatcaca
     1141 tgcactggtc aaagagctgg cttatattcg tcgcgaaggc aaagtgatga cctatcttcg
     1201 tcccgatgcc aagtcgcagg ttaccattga gtacaatgat gacaatcagc ccgaaaaagt
     1261 tcacaccatt gtggtttcca cccaacacga tgaatttatc aaacccacag atactaaaac
     1321 acaggaacag gctgatgaag aaatgctttc aatcatttat aacgatgtaa agaacttact
     1381 tattccccga gtaaaagcca aagttattca gtttgatcaa ttgatcaagg atgatttcgt
     1441 gctgtatgtt aatcctaccg gcaagtttgt aatcggtgga cctcatggcg ataccggact
     1501 gacaggccga aaaattatag tggataccta cggtggtaaa ggcgcacacg gcggtggtgc
     1561 attttcaggc aaagacccat cgaaagtgga tcgttcggct gcctatgcaa cccgccatat
     1621 tgccaaaaac ttagtggcag caggtgtttc cgacgaagtg ctggtgcagg tggcttacgc
     1681 tatcggagtt gccaaaccga tgaacattta tatcaataca tatggcaaat cgaaagttag
     1741 tctttccgat gcacaaatag ctgaaaaagt tgaaaaactg tttgatatgc gtcccaaagc
     1801 cattgaagag cggcttaaat tgcgcactcc gatgtatgaa gaaacagctg catacggtca
     1861 tgtgggtcgt acaccggtta ctaaggaaaa gatatttaaa gacggaaacg gtaatatatt
     1921 taagcaaaca gtagatttat ttacctggga aaaactggat atggtcaatg aaattaaaaa
     1981 ggaatttggt ttataatatt ttctctgaaa aaaaacgaaa gagcttcaac attacgaagc
     2041 tctttttttt ttatgtgaaa atataccgaa aacagaattt attgaacttg atattatttc
     2101 atttttttag attacaatat cggagattac acctgatatt tcaaacattt ccattacttt
     2161 tgcgtgaaaa atcctaccaa tatgaaccgt gacgaaattc gcaattatat tatccaaatg
     2221 tatgaaacac tcaaaaaatg gttgtttcag tttatcgatt ggcgtgaacg tcgaatcgtt
     2281 cgtttttacg gtttacccaa atggaagaag acagccaata ccattcttac ttttttctgc
     2341 ctgttcctgt tttacctttt aatagtagat attaattttt tgtggctttt tggtaagtca
     2401 ccctctctga gagctatcag caatcctccg caatccttat catccgaaat aataagtgct
     2461 gataaccagt taattggaaa atatttcaat gaaaaccgca agttggttaa gttcgaagat
     2521 atttcaccgg ccctgatcaa tacgctggtc agtaccgaag accgacggtt ttacaagcat
     2581 tttggcgttg atattcacgg actttttggc gccatgaaag atatggtgca gggcaatccg
     2641 cgcggtgcaa gtaccattac ccagcagttg gtcaagaata tgtacaaaac ccgtagtcag
     2701 tattcccgcg gcttgcttgg ctctatgccg ggcttgcgac tgccaattga aaagacaaaa
     2761 gaatggactg ctgccctgaa actcgaactt ttttattcca aaaaagatat tctggaaatg
     2821 tatctcaata cagtgagctt cgggagcaat gcttacggga tatttacagc ttccaaaaca
     2881 tatttcaata ccactccgga caaactcagt tatgaacaat cggccgtgct ggtagggttg
     2941 cttaaagcta tcaccaagta taacccttac ctcaatcccg aaaactccat taaccggcga
     3001 aacgtagtac tcagccagct tgccggcaat aaatacatca ctccgcaggt ggccgattcg
     3061 ttaaagaaaa ttcctcttcg cctcaattac aacatcgaaa agcccaacga aggaattgct
     3121 ccctatttca gggcaaatct gacccggttt cttgaaaaat ggggagaaaa caacggatat
     3181 gatatttatg ccgatggatt gaaaatatat gtaactatcg attcgcgtat gcaaaaatat
     3241 gccgaagaag cagtcagaaa acagatgcga cagttgcagc gcaatttttt tgaacactgg
     3301 tcggggcaaa atccatggcg cgatgaaaat ggaaacgaac tgaaaaattt cattcccgaa
     3361 atagccaaac gcacaaaggt ttatcgtcaa ctggctacta aatacgctaa taattccgat
     3421 tcaataaaca aatacatgaa cattccccga aaaatgaagg tttttgatta cgataaaggg
     3481 gaaaagacag tcaccatgag ccctatggac tccattcggt atatgaatca cttcctgcat
     3541 tgcggatttc tggctatgga acccgatacg aaatatgtga aagcctgggt gggcgatgta
     3601 aattacgatt actggcaata cgataaggta gcacaaagca aacgccagcc gggatctact
     3661 ttcaaacttt tcgtttatac agcagcactg attgccggaa aatctccctg cgacaaagta
     3721 gaggacaaac catttgtctg gggcacgggt aaatatgcct ggcgtccgaa aaatgcaaat
     3781 ggaggatatt cctatgccga tttatcgctc aaaagagcat ttgcaaaatc ggtaaatacg
     3841 gtagccgcac aattagccaa agaagtaggg attgcaaatg tggccaaatg tgcacatctg
     3901 ctgggtataa aatccaaact cgaagaagtg cccaccttaa gccttggcgc ttccgatgtg
     3961 acgttgctcg aaatggtcaa ttcatacagc accattgccg gagaagggat gtatcattct
     4021 cccataattg ttaccaaaat agtggataag gacggttatg tcatttacga agaaaaaatt
     4081 aaaaccaaac gcgttatttc atatgaaaac gctttcctga tgacagaatt actcaaagga
     4141 ggtataactg aacccggcgg aacctcacgc gctctctgga actacgacct gatgagatgg
     4201 gacaccgaat ttggcggaaa aaccggaacc tcatccaact attctgatgc atggtatata
     4261 ggcatcacac ccaaactggt tgccgggtcg tgggttggtg ccgaacaccg gagcgttcac
     4321 ttccgtagtg gaagcatggg gcagggaagt cgtacggcgt tgccggtatt tgctttattt
     4381 atggaaaaag tgttatcgga taatgattta gcacaatatc gggcaaaatt tcctgcagaa
     4441 cctaaacaga ctattacccg ggattacaaa tgccaaagct attacagaac cgatacagtg
     4501 gctgaagaca gtatcgatgt ggaaccaata gagtcgttac ccgtaccgga tctgaacatg
     4561 ggaactgaaa cagaaaagcc agctcccgta ttgccttgat aatcaaaaga tattacaaat
     4621 ttgctaatcg gatgttaacc ggttagctgt tttatgaaat aattaaacag atgaataaaa
     4681 taatcagtaa agagtatttt tcggccaatg tggtaaaatt taccgtcgat gcgccactta
     4741 tagccaaagc acggcgggca ggtcactttg ttatagtaag ggtaggtgag aagggtgaac
     4801 gcattccact caccatagct gatgccgata tcagtaaagg aaccatcacc ctggtggttc
     4861 aacgtatggg agtatcttcc accaaacttt gtgccctgga agccggtgat tatattaccg
     4921 atctggtggg accactggga aaagccactc atatcgaaaa tttcggtacc gtagtgtgtg
     4981 cctgtggtgg tgtgggaaca gctcccatga tgccaatagt ggctgccctc aaaaaagccg
     5041 gaaacaaagt aatcaccgtt attgctgctc gtaccaaaga cctggtaata ctcgaagagc
     5101 aaatgcgtga aaattccgat gaagtgattg tgatgaccga tgatggctct tatggtaata
     5161 aaggattggt aaccaacggg gtagaagctg taattcaacg cgaaaaagtg gacctgtgtg
     5221 tgactattgg tccggccatc atgatgaaat ttgtgagttt actcactaag aaatatgata
     5281 ttccaacagt tgcttccctg aatacaatta tggtggacgg aaccggaatg tgtggagcct
     5341 gccgggtaac catcggtgga aaaacaaagt ttgtttgtgt ggatggacct gaatttgatg
     5401 ctcatcaggt agattttgac gaaatgctga tgcgactcgg tggttacaaa gatcaggaaa
     5461 aagaagaaat ggaacgtaaa attttgcata ctaaataaaa tcaatgaata caagagatga
     5521 acaatggcgc gtagatttac gggcaaaaat caaagctaag gatagaacaa atctgacacg
     5581 ggttcacatg ccggaactcg acccggaata ccgcagtcat attcgcaatg aagaggttaa
     5641 tctgggtctg acaccagctc aggctatggc cgaagctcag cgctgtctgg actgtgttaa
     5701 tcctacctgt atggaaggtt gcccggtgag tattaaaata cctgatttta tcaagtttat
     5761 tgaaacgggt gagtttctgg aagctgcaaa agtgctgaaa atgacttcgg cgctacctgc
     5821 tgtatgcgga cgggtttgtc cgcaggaacg tcagtgtgag gaaaaatgta tacatgtgaa
     5881 gatgaagaaa gaatctgttg ctatcggaca tctggaacga tttgcagctg atttcgaacg
     5941 ggaaagcggt cagatttccg tacccgcaac tgcccctgcc aatggtatta aaattgcagt
     6001 ggttggttcc ggacctgctg gtctggcttt tgccggcgat atggcaaaac ttggttatga
     6061 ggtggtggtt tttgaagcat tgcacgaaat aggcggagta ttaaagtatg gaattccgga
     6121 atttcgcctg ccaaataaaa tagtggatgt ggaaatagac aacctgaaag cgatgggtgt
     6181 gacatttatc aacaactgta tcattggtaa aacaatagcc attcaggaac ttgaagaaaa
     6241 tggttataag ggcatatttg ttgccagtgg agccggtctt ccccgattta tgaatatcaa
     6301 aggcgaaaat ctggtgggcg tcatgtcatc caacgaatac ctcacccgtg taaacctgat
     6361 ggatgctgcc aatccggaat cggatactcc ggtgtataaa ggcaagaaag tggcagtaat
     6421 cggcggtggt aatacggcaa tggattctgt tcgcactgcc cgaaggctgg gagctgaaaa
     6481 agccattatt gtgtatcgtc ggtctgaaga ggaaatgcct gcacgactgg aagaagtaaa
     6541 acatgccaaa gaagaaggca tcgaatttct cactcttcac aatccgatag aatatatcgg
     6601 gaatgaaaca ggacgagtaa ctcagatggt attgcagtta atggaacttg gtgaacccga
     6661 tgcttcggga cgccgctcac cggtagaaat tcccggaaaa acccggattg tggatgtgga
     6721 tgaagtgatt gtgagcgtgg gtgtatcacc caatccgttg atcaaacaat cggttccgga
     6781 actcgaaacc accaaatggg gtacactggt agttaatcag gataccatgc aatcggtttc
     6841 actgcccata atatttgccg gtggtgatat agttcgcggt ggcgctacgg ttattctggc
     6901 tatgggcgac ggacgacgcg ctgccaaagc catggatgaa tggattaaaa ataaatcata
     6961 agactttctt attcttagcg aattaaaaaa acgacgaaat tcaaattgga tttcgtcgtt
     7021 tttagtaatt tatatttttc tcaaaaatta taaaccacag cacccactat tcgatgatta
     7081 ttgatggagt aggggtttgt tactttaccc tcagcgttaa gttttccata ttcggcattc
     7141 gttagatcat attccagacc aagccggatg tttttaaaat tatatgaaaa gtgcggtgct
     7201 acccggaata attgattaag atgctggtta ccagaaaaat atccagaact atagactgta
     7261 aaatcaccgg tggatgatgg cagtaaattc ttgtcggtac caagatttcg gaggtaacct
     7321 ccaaaaatac ctacttgcca ggtttttcca tacacgagat tgatccagct gcccgattgg
     7381 ttgaaattgg tataggtagc cctcttatta tcagcatcat atccacttat gccatatccc
     7441 atgggcattt gtaaatcact caaattttga ccaagcactg attttgcttt taactgaaac
     7501 attttattgg tgtaatttcc ataaatcatt acacttttcg ataaaagtct gctatctgct
     7561 ttaatcgttt tcacatcaag ccc
//
LOCUS       DAOPZK010000093         7431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_12056_length_7431_cov_30.312093, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000093 DAOPZK010000000
VERSION     DAOPZK010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12056_length_7431_cov_30.312093"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3026)
                     /locus_tag="PKH68_06565"
     CDS             complement(<1..3026)
                     /locus_tag="PKH68_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX12636.1"
                     /translation="MKLKTLVKQITVLFFLTVLPVWAIGQTITVRGTVTDDQGGTLLG
                     VSVKEVGTTNGASTNNDGKYELRVNSNASLLFSYIGYKNQTVQVNNRSVINIVLQQDV
                     LGLDEIVVVGYGTQRKEAVTGSVASIRGDIMREIPSTNMSQALQGRIAGVEMSQTSSK
                     PGAEMQIRIRGTRSLTASNDPLVVLDGIPFAGNISDINPNDIKSIDILKDASATAIYG
                     SRGANGVLLVTTNKGLTGQKARVTYNGYYGSSQVFSKVDMMDGDQFAAMRAFANMSAY
                     KTNTADEVAGTNTDWQDLFYKTGTLQSHDVSVAGGTDGGSYNFGIGYYKNEGVVPLEG
                     YSRLSLRGSLDQEIGKIFRIGFTTNSNYNVTESASTGGLYQVLQNTPLINPYNEDGSW
                     KRTVDMPADKGVWVYTRDIVEAKRDLMLSQTKGFGSYNTVYGEVKVPGVEGLKYRINV
                     GLNFRTSTGGSYTGPGINATSETNPSGASVSNSLRTSWAVENLLTYDRTFAGKHQVNA
                     VAMYSAEENLYNRSRMTAKGVASNFQWYNLGMTDGERSILPGDQQYEKSGLISYMGRV
                     MYSYDSRYMISATLRSDASSRLAPGHQWHTYPAVSVGWNLKKEAFMSNVNWLDNLKLR
                     VGYGQTSNQAVAPYATLGGLSTRPYNFGDATANFVTGTYVSTLPNPNLGWEYSNTWNY
                     GVDFSLLKNRLSGTFEYYVTNTKDLLLSVNLPQTSGVGSFMANVGATQNKGFELSLNA
                     VILDNLNGWNWTAGVNLYTNQNKIVSLASGNLKDEGNAWFVGSSINSIYAPKKIGLWQ
                     EGDPFLNILEPGGNVGMIKVEYLGDYYKEGDVLPKGSQIGDPVRAIDGGGKDRQVMEL
                     DPDFQGGFNTTVGYKGLDLTVIGAFKSGGTLISSLYSANGYLNMLSGRRGNVDVDYWT
                     PDNTDAKYPKPGGLMSGDNPKYGNTLALFDASYMKIRTITLGYNFKQRAVKDLGIQNL
                     RLYATLQNPFVLFAPYTSETGMDPETNSYGDENMASA"
     gene            complement(3317..4927)
                     /locus_tag="PKH68_06570"
     CDS             complement(3317..4927)
                     /locus_tag="PKH68_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HNX12637.1"
                     /translation="MKKLIFAFIYLLLSLSVGAQSDIPYLKKAGTATQLIVEGKPFLM
                     LGGELGNSSTADAAYMQHIWLQLKAMNLNTALVPVYWELMEPEEGQFDFSLVDEMIAG
                     ARENNLKLVLLWFGSWKNSMSCYAPGWVKKDFRRFPRTRDRNGVPSEILSPFFSANLQ
                     ADSNAFEALMKHLKSVDEADRTVIMVQVENEIGQLPEARDYSPAATKLFLSAVPESLM
                     NYLQKNQRDLLPHITDLWANSNFSKKGTWQEVFGKSLATDELFAAWYFAQYTDRIARA
                     GKEIYNLPMYVNCALNRPGVEPGKYPGGGPLPHLINIWQAGAPHIDMLSPDIYHGDFR
                     HWCRLYDTLKNPLFIPEIKMEPENAAQVFYVIGRHKALGFCPFSIESASENEKIPLSN
                     SYRVLGDLTDLIMEHRSGTDGVYLDKENQSDTIPIGDYQLIISHVSTLPWSDGAKAEK
                     WTPAGCIIIQTAPDEFWIGGTAIACTFRNLKNKKVVAGILSADICTKSDKNWKFRRLN
                     GDQTHQGRHIRISSDNWEIQRVKLYDFN"
     gene            complement(4928..7399)
                     /locus_tag="PKH68_06575"
     CDS             complement(4928..7399)
                     /locus_tag="PKH68_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNX12638.1"
                     /translation="MKLIIQKKNFLLLAGALISISVNAENPETRKLTMWYDKPAEVWN
                     EALPIGNGRLGAMIFGDPSNEKIQLNEGTFWSGGPSRNDNPNALGALPEIRRLIFDGN
                     FREAEKLVSQNMMTPRNGSMYQVVGNLTLFFPGHEKYTGYYRQLDLKNAVFTSGYSVD
                     GISYKREIFASQPDQVIVVRLTAGKSGKLTFDAGMDGPLKKQVKVVDGNTLEMNGLSS
                     SHEGIEGQVKFNSRAKIIARGGSITAGTNNIRISNANEVLILVSIATNFVDYQTLTAD
                     EVSKCKSYLENAEKLSYSKLLKKHVSAYQKYFNRVDFDLGTSPASLQPTDIRIRNFAS
                     SSDPELISMYYQFGRYLLISSSQPGGQPANLQGIWNGSTNPAWDSKYTVNINTEMNYW
                     PAEKSNLAEMHEPLLKMVKELSQTGQKTAKDMYGARGWVVHHNTDIWRITGVVDGPTW
                     GMWPMGGAWLTQHLWEKYLYSGDLEFLRSVFPVMKSCCEFYQDFLVEEPVHKWLVVSP
                     SISPENSPAGRPSLCAGATMDNQILFDLFTKTLRAAELLGEDKALKDEYRKIISRLPP
                     MQVGRFGQLQEWMEDLDNPDDQHRHVSHLYGLYPGNQISPYSNPELFDAARTTLIHRG
                     DVSTGWSMGWKVNFWARLLDGNHALKLIKDQLTLVDPVNGGYNGGTYPNLLDAHPPFQ
                     IDGNFGCAAGISEMLVQSHDGAIQLLPALPDEWRDGKLSGIRTPGGFEISFEWKDGKI
                     HKMEILSTLGGNCRIRVPNELILVKKYGIKPANGDNPNPFFENPEIKTPLLAENIKMN
                     KVSLNNTYLYDLPTKPGKKYQLSKL"
BASE COUNT         1929 a         1725 c         1517 g         2260 t
ORIGIN      
        1 gctgaagcca tgttttcatc accgtaagag tttgtttcgg gatccatacc tgtttcgctt
       61 gtgtaaggag caaataatac aaatggattt tgtaatgtag cataaagcct caaattttga
      121 atgcccaaat ccttaacagc tctttgttta aagttatatc ccagtgtaat ggtgcgtatc
      181 ttcatataag atgcatcaaa taaagcaaga gtattaccat atttcggatt gtcaccactc
      241 atcagaccac ctggtttagg atattttgca tctgtattgt caggtgtcca gtagtcaaca
      301 tccacatttc ctctacgtcc tgataacata ttgaggtaac cgttggcaga ataaagtgaa
      361 ctgataagcg taccaccgct tttgaaagcg ccaattacgg tcaggtcaag tcctttgtaa
      421 cccacagttg tgttgaaacc gccctgaaaa tcaggatcta actccataac ctgacgatct
      481 ttaccgccac catctattgc tcttactggg tctcctattt gactgccttt aggtaaaaca
      541 tccccttctt tgtagtagtc gcccagatat tcgaccttaa tcataccgac gtttccaccc
      601 ggttcaagga tattcaaaaa tgggtcgcct tcctgccata atccaatttt tttaggtgca
      661 taaattgaat taatagagct tcctacaaac caggcgttac cttcatcttt caggtttcct
      721 gatgcaagag atacaatttt gttctggtta gtatacaagt ttacacctgc agtccagttc
      781 catccattga gattatccag tataacggca ttaagtgata attcaaaacc tttattttgg
      841 gttgctccaa cattagccat gaagctgccc acacccgatg tttgaggaag gtttacactc
      901 aacagaagat cttttgtatt ggttacataa tattcaaatg taccggacaa gcggtttttc
      961 agcaaagaga aatccacacc ataattccag gtattggaat actcccatcc taaatttgga
     1021 tttggtaatg tacttacata tgttcctgtt acaaaattag cagtagcatc accaaagtta
     1081 tatggacgag tacttaatcc gcccaacgtg gcgtaaggag caacagcctg attggatgtc
     1141 tgaccatagc ctactctcag tttcaggtta tcaagccagt ttacattgct catgaatgct
     1201 tctttcttca ggttccatcc tactgataca gcaggataag tatgccactg atgtccgggt
     1261 gccagtcttg aagacgcgtc agagcgaaga gtagccgaaa tcatataacg actgtcgtac
     1321 gaatacatta cacgtcccat atacgaaatc aaaccactct tttcatattg ttgatcaccc
     1381 ggaagaatgg atctttcacc atcggtcata cccaggttat accattggaa gtttgaggca
     1441 acaccctttg cggtcattcg tgaccgatta tacaaatttt cctcagcaga atacattgcg
     1501 acagcattta cctgatgttt accggcaaaa gtccggtcat aagttagcag gttttccacg
     1561 gcccagctcg ttctcaacga gttacttacc gaagcgcctg aaggattggt ttcggatgta
     1621 gcgtttattc ccggtccggt gtagcttcca cctgttgagg tgcggaaatt cagacctaca
     1681 ttgatacggt attttaagcc ttcaactccg ggtactttta cctcaccata aacagtattg
     1741 tacgaaccga aacctttggt ttggctgagc ataagatctc tttttgcttc tactatatct
     1801 cttgtataca cccatacacc tttgtcagcg ggcatatcta cagttctttt ccagcttcca
     1861 tcttcattat aaggattaat aagtggtgta ttctgtaata cctgatataa tccgcctgtg
     1921 ctggctgatt cggtaacatt gtagttgctg ttggtcgtaa agccgatgcg gaagatttta
     1981 ccaatttcct gatcaaggct accgcgcaat gagagacgac tatatccttc cagcggcact
     2041 acaccttcat ttttgtaata acctatccca aaattatagg atccgccatc tgttccgccg
     2101 gcaacactta catcatgact ttgtaaggta ccggttttat aaaacaggtc ttgccagtct
     2161 gtgtttgttc ctgcaacttc atcggcagtg ttagttttgt aggcagacat gtttgcaaaa
     2221 gccctcattg ctgcaaactg gtctccatcc atcatatcaa ctttggaaaa tacctgactt
     2281 gaaccatagt aaccgttata agttaccctg gctttctgac cggttagtcc tttattagtt
     2341 gtcacgagga gtacaccgtt tgcacctctt gagccataaa tagccgttgc tgaagcatcc
     2401 ttcaaaatat caatactttt gatgtcattc ggattgatat cactgatgtt tccggcaaaa
     2461 ggaataccat ctaacactac caacggatca ttactggctg tgagtgaacg ggtgcctcga
     2521 atacgaattt gcatttcagc tccgggtttt gaggaggttt gtgacatttc gacaccagca
     2581 atacgacctt gcaatgcttg tgacatattc gtggaaggta tttctctcat tatatcacct
     2641 cttattgaag caacagaacc ggttacggct tccttgcgtt gtgttccata acctacaact
     2701 acgatttcgt ccagtccaag cacatcctgc tggagtacta tattgataac agaacgatta
     2761 ttgacctgaa ctgtctgatt tttatatcca atatatgaaa aaagcagcga tgcattgcta
     2821 ttcactctca gttcatattt tccatcgtta ttggtacttg ccccgttggt agtacccact
     2881 tcctttacac tcacaccaag gagtgttccg ccctggtcgt ctgttacagt tcctctcaca
     2941 gtgatggttt gaccaatggc ccaaacagga agcaccgtta aaaagaagag aacagtaatt
     3001 tgttttacta atgtttttaa tttcatggat ttaatttctt tgatttacaa taaagttaat
     3061 tttttgtgtt ttactcagaa aaaggggtct ttcgtatttt aagttttcat gatcatttta
     3121 gtttttgcag tttggtatat ttggcattta gcacgtttta tatttaatga ttatcaaatg
     3181 tacggtcaat ctgatagctt aatattcata tttagtacaa atgcttatag atttgtaaca
     3241 ccggttgaac atttgttaca tttgttttca aactgcatcc atgtattatg aaaacggctt
     3301 tttttatgtc ttttttttaa ttaaaatcat agagttttac ccgttgtatt tcccagttat
     3361 ctgacgaaat tcgtatatgc cgcccctgat gtgtctgatc tccgttcaat cttctgaatt
     3421 tccagttttt atcagattta gtacaaatat cggcagataa aattcccgct acgacctttt
     3481 tatttttcaa atttcggaaa gtacaggcta ttgctgttcc cccaatccaa aactcatcag
     3541 gtgcagtttg tatgatgata cagcctgccg gcgtccactt ttctgctttg gcaccatcgc
     3601 tccagggcag tgtggatacg tgtgatatta taagttgata atcgcctata ggaattgtgt
     3661 ccgattgatt ttctttatcg agatatactc cgtcggtgcc cgaccggtgt tccataatca
     3721 ggtcagtcag gtctccaagc acccgatagc tgttggaaag ggggattttt tcattctccg
     3781 aagccgattc gatggaaaaa ggacaaaatc cgagtgcttt gtgtcgtccg atcacataaa
     3841 aaacctgagc agcattttcg ggctccattt ttatttcggg tataaacagg ggatttttca
     3901 gcgtatcgta gagccggcac caatgccgga aatcgccgtg ataaatatct ggagataaca
     3961 tatcgatatg tggtgcccct gcctgccaga tattgatgag atgaggcagc ggaccaccac
     4021 ccggatattt tccgggttcc acgcccgggc gattcagagc gcagttgaca tacatcggca
     4081 ggttgtaaat ttcctttccc gcacgggcaa tccggtcagt gtattgcgca aagtaccagg
     4141 cagcaaataa ttcatcggtg gccaggcttt tgccaaacac ttcctgccag gttccttttt
     4201 tgctaaaatt actattcgcc cataaatcag taatgtgagg aagtaaatcc ctttggtttt
     4261 tttgcaaata attcatcagg ctttccggta cggccgatag gaataatttg gttgcggccg
     4321 ggctgtagtc gcgggcttca ggaagctggc caatttcgtt ttccacctga accatgatga
     4381 cggtccggtc agcttcgtcc actgatttga ggtgtttcat aagagcttcg aatgcattac
     4441 tgtcagcctg aagattagca ctgaagaacg ggcttaggat ttctgacggt acaccgttac
     4501 ggtcgcgggt acgcggaaac cgtctgaaat ctttcttaac ccaacccggc gcatagcacg
     4561 acatgctgtt tttccagctg ccaaaccaga gaagaaccag tttcaggttg ttttcgcgcg
     4621 caccggcaat catctcatca accaaagaaa agtcaaactg accctcttcg ggctccataa
     4681 gttcccagta aaccggtacc aatgccgtat tgaggttcat cgctttcagt tgcagccaga
     4741 tgtgctgcat gtacgctgca tcagcagtgg aagaattgcc gagttcgcct ccaagcatca
     4801 aaaatggttt tccctccaca ataagctgag tggcagttcc tgctttcttc aggtatggaa
     4861 tatcactttg tgccccgaca gaaagcgata gaagaagata tatgaatgcg aatattaatt
     4921 ttttcattta taatttgctc agctggtatt ttttccccgg cttcgttggc aaatcgtaca
     4981 ggtatgtatt gttcaatgat accttattca tcttaatatt ttctgctaac agcggagttt
     5041 ttatttcggg attttcaaaa aaaggattgg gattatctcc atttgcaggt tttattccat
     5101 atttttttac caatatgagc tcgttcggta cgcgaatacg gcaattacca cccaatgttg
     5161 atagaatttc catcttatga attttaccgt ccttccattc aaagctgatt tcgaaaccgc
     5221 ccggtgtcct tatcccgctc aatttgccgt ctctccattc gtcgggaagt gccggaagca
     5281 gttgtattgc gccatcgtgg ctttgcacaa gcatttcgga aatacctgct gcacacccga
     5341 aattaccatc tatctgaaaa ggcggatgtg catccagcaa attgggatat gttccgccgt
     5401 tgtatccgcc attcaccggg tcaaccaggg tgagctggtc ttttatcagc ttcaatgcgt
     5461 ggtttccatc gagcagtctg gcccagaaat tcaccttcca tcccatagac catccggtgg
     5521 aaacatcacc ccggtgtatc aatgtggtac gtgccgcatc aaaaagttcg ggattggaat
     5581 aaggtgaaat ctgatttccg ggatacaatc catacaggtg agatacgtgt cggtgctggt
     5641 cgtccgggtt atccagatct tccatccatt cctgcaactg accaaaccgg cctacctgca
     5701 tgggcggcaa acggctgatt attttccggt attcatcttt taacgcttta tcctcaccga
     5761 gcaattcggc tgcccggagg gtttttgtga acaaatcaaa caggatctga ttatccatcg
     5821 tggctcccgc acaaagagac ggacgacccg ccggcgaatt ttcaggtgat atcgacgggc
     5881 tcactaccag ccatttatgt acaggttctt ccaccagaaa gtcctgataa aactcacagc
     5941 aggatttcat cacaggaaat accgatctga gaaattccaa atcaccgctg tacaggtatt
     6001 tctcccacag atgttgagta agccaggcgc cacccatggg ccacataccc catgtgggtc
     6061 cgtcaaccac gcctgtgatg cgccagatat cggtattgtg atgcaccacc caaccacggg
     6121 cgccatacat gtctttcgct gttttttgcc cggtctgaga caattccttc accattttca
     6181 gcagcggttc gtgcatttct gcaagattgg atttttcggc tggccaataa ttcatttccg
     6241 tattgatgtt gaccgtatat ttgctgtccc atgccggatt tgtgcttcca ttccagatac
     6301 cctgcaggtt agccggttgg ccgccgggct gcgaactgga aataagcaga taacgaccga
     6361 actgataata catggatatc aactccggat cagaggacga tgcaaaattc cggatgcgaa
     6421 tatctgttgg ctgaagtgaa gccggagaag tacctaagtc aaaatctacg cgattgaaat
     6481 atttttgata tgcggaaaca tgctttttta ggagtttgga ataagataat ttttcggcat
     6541 tttcaagata ggacttgcat tttgacactt catcggcggt caaagtctga taatccacaa
     6601 aatttgtagc gatagaaaca agtatgagta cctcgttggc attacttatc cggatgttgt
     6661 ttgtcccggc tgtaatcgaa cctccgcggg cgatgatttt agcacgggag ttgaatttga
     6721 cctgaccttc aatgccttcg tgtgaagatg aaagtccgtt catttccagc gtattaccgt
     6781 caactacttt tacctgcttc ttcaacggac catccatacc tgcatcgaat gtcaactttc
     6841 cggatttacc ggcagtcaaa cgtaccacaa tgacctgatc gggttgtgag gcgaatatct
     6901 cacgcttgta acttattcca tctactgaat atccggatgt aaaaacggca tttttcaggt
     6961 caagctgccg gtaatatcct gtgtattttt cgtgtcccgg aaaaaaaaga gttagatttc
     7021 caacaacctg atacatagac ccattacgcg gtgtcatcat attctgactg accagttttt
     7081 cagcttccct gaaatttcca tcaaaaatca gccggcgaat ttccggcaat gctcccaatg
     7141 catttggatt gtcgtttcgg gatggtccgc ccgaccagaa agttccttcg ttcagctgaa
     7201 ttttctcgtt ggaggggtcg ccgaagatca tggcacccaa ccggccgttg cctatcggca
     7261 gcgcctcatt ccaaacttca gccggcttgt cgtaccacat cgtcagtttt ctggtttcag
     7321 gattttctgc attcacggaa atggaaatta aagctcccgc gagcagcaga aaattttttt
     7381 tttggataat taatttcatt gtaaataatt tataaatctg ccccctaacc c
//
LOCUS       DAOPZK010000094         7428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_12060_length_7428_cov_25.255934, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000094 DAOPZK010000000
VERSION     DAOPZK010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12060_length_7428_cov_25.255934"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PKH68_06580"
     CDS             complement(<1..152)
                     /locus_tag="PKH68_06580"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNX12639.1"
                     /translation="MSFIAEKIQFEGFTFDDVLLVPAYSEVLPRNVDLTTRFTRNIEL
                     KIPIVSA"
     gene            complement(303..1019)
                     /locus_tag="PKH68_06585"
     CDS             complement(303..1019)
                     /locus_tag="PKH68_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX12640.1"
                     /translation="MKKAIVVGASSGVGRELAKILVENGYRVGITARRENLLHELAAE
                     KPDAYLVKPFDLINTEIILPNLEELVRELGGLDLLVISAGLIKYNTKLDYDIEYTTNL
                     VNVTAFTLISNWAFNLFRNQGTGHLVGITSVAGLRGWRNNVAYNASKGYQMNYLEGLK
                     NLARHEKLPITVTNIIPGYMETNMKGTTMVFWVCPPDKAAQLIFKAIRKKRKIAYVYS
                     RWRIGAFLYKHLPNKVIERA"
     gene            1328..2158
                     /locus_tag="PKH68_06590"
     CDS             1328..2158
                     /locus_tag="PKH68_06590"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX12641.1"
                     /translation="MMDELKIAKVDIGQIVRSEFAGKKIPGFFIKILKWIGHEEGLNK
                     LFASAPGKKNLDFVDACMDFYRIKCNVIGLENLPSDGKPLIFVSNHPLGALEAICIAH
                     VIGHRYNGKINFYANEILTWVEPLKEKFIPVSKKGYQNRESVKKVQEFFQSDEHVVIF
                     PAGATSRKYGNKIIDLEWHKNFVQKSVQYQRDVIPLYFKGKNSTFFYLLEKFREMIRS
                     KVNFEMLFLVDEIFKQRGNTFTLYIGKPVGWQTFDNTKTPGQWAQTVKKMVYKLPENE
                     "
     gene            2234..2794
                     /locus_tag="PKH68_06595"
     CDS             2234..2794
                     /locus_tag="PKH68_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNX12642.1"
                     /translation="MKSVPKFLFIIIAVMVSVIAGTMGVSGQNAASAEDRQYLVKVGD
                     MAPDFEMKLPDGSKVKLSSLRGKIVMLQFTASWCGVCRKEMPHIEKEIWLKHKNNPNF
                     ALYGVDRDEPASKVKQFAKDTGVTYPIGLDPKAGIFGLYALKEAGITRNVIVDENGKI
                     VMLTRLYDEQEFAGMVSLIDKLLAEM"
     assembly_gap    2821..2920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2921..3226
                     /locus_tag="PKH68_06600"
     CDS             <2921..3226
                     /locus_tag="PKH68_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNX12643.1"
                     /translation="MHLSQFAGWILPGLGFVLPIAMWLTNKDENENVDKHGKNILNFT
                     ISYFIYAAISGILVIIAIGLALLAVLGVVVTIVIIIAAVKAYNNEYWDYPLTIKFLK"
     gene            3295..3948
                     /locus_tag="PKH68_06605"
     CDS             3295..3948
                     /locus_tag="PKH68_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNX12644.1"
                     /translation="MKIPIPITVTYIDIHTHKPEMQTGELTILNVSLSEAGKVLSLSE
                     NRLFSLGIHPWNADNIFENDTEQLKLWVASKWVRLVGECGLDKLAKAGYDIQLHYFKK
                     QIELSEQFQKPLIVHCVGYYNELFDLRMQLKPAQKWIIHGFRGKPQLAEQALKAGCDL
                     SFGEHFNAESVEITPTDRLYVETDESSLPIAEIYHRIAQIKGCEADQLTAGAKLLNC"
     gene            4061..5674
                     /locus_tag="PKH68_06610"
     CDS             4061..5674
                     /locus_tag="PKH68_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX12645.1"
                     /translation="MRKYSLFPLLFLATTLFSQGISPVQQRKLVQALSAISSLYVDTI
                     NDTKLVESSIRGALKDLDPHSVYTPKEEVQRMREPLEGSFEGIGVQFQILNDTIMVVQ
                     TIAGTPAEKVGVLPGDKIVYINDELVAGSASKVQNSDVFKRLRGKKGTEVLVKIKRGN
                     LPELIEFKIIRDKIPIYSVDATYMADSKTGYIKINSFGSTTFDEFNKALENLKKEGMK
                     DLILSLQGNGGGFLGTAIQIADEFLPAKKMIVYTQGNNQPRTDATATVTGNFEKGRLV
                     VLVDEFSASASEIVSGALQDWDRAVIVGVRTFGKGLVQREFPLVDGSLMRLTVARYYT
                     PTGRSIQRPYKDGTESYYKELSNRFRNGELMHADSIHFPDSLIYKTLINGRTVYGGGG
                     IMPDVFVPVDTTEYSDFHRSMVAKGVVSRTAVEYLNSHRQELKEKYAAFQKFKKQFET
                     GDDFLKLLLAEAEKEKIKYDDVQFNRSKRLIKLQLKALVARDLWNINEYYQVMDSENE
                     SLQKALEILNTPGAYDKILTISKLYSQQK"
     gene            5678..6274
                     /gene="pnuC"
                     /locus_tag="PKH68_06615"
     CDS             5678..6274
                     /gene="pnuC"
                     /locus_tag="PKH68_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNX12646.1"
                     /translation="MQEYLRAHWVEIAGALLSVVYLVLSIREKAGLWIFGFISSAFYV
                     FVFFESKFYADMSLQFYYLAVSVFGWINWHRKTTGSDSAIIPTTTIARTQYSAYIAAI
                     VVTYLIYYVILKYWTDSPVPVLDSLVGALSVVATWMLARKKIENWLLWIAADALAAGL
                     FLYKGLYPTVVLYVIYTSMAVVGYFQWKKHLQITSSEI"
     gene            complement(6282..>7428)
                     /locus_tag="PKH68_06620"
     CDS             complement(6282..>7428)
                     /locus_tag="PKH68_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNX12647.1"
                     /translation="PNNKKVTSVPFEMAGSYVVIKLTINNSTPLSMILDTGVKNTIIN
                     ELMEGDDVPLNKTTTDSTLIQGLGNNQGIRAYRSYKNDLKIGNLIFNNKVVLLLPENI
                     FNFTNYTGKKINGILGSDILKDYVVEINYTKLRVYFYNSDTFVVPGKYSSIPATVVNN
                     KMYVDALYTGADSVPRNVRMLVDTGAELGSWLFNSSISSMPKKKVYGYIGEGLSGEIK
                     GYYARSVQLCLGPHCITNPVVTFPDSAYTRGLEQETSRDGTLGNQTLKRFNCIIDFKT
                     PAFYLKPNLMFKDKFRYNVAGIEILREMPHFYITRVYKVWKNSPADVMGVREGDLILE
                     VNGMKAFQMNVSEIRDIFETSRRFPMHIVIQRGNDVIDLDLNMNSKI"
BASE COUNT         2128 a         1476 c         1626 g         2098 t
ORIGIN      
        1 gcagatacaa tgggtatttt cagttcaata tttcgggtaa atcgggtggt gagatccaca
       61 ttgcgcggca atacttcgga gtaggctgga acaagcaata catcgtcgaa cgtgaatcct
      121 tcgaattgta ttttttcggc aataaatgac atagtaagta ttttagagga ttattgatga
      181 aaagctatga aaaatattgc gtgcaaattt agtcattttt tgcgggttag cccttgtttt
      241 aatgtaaaaa aatagaaatt aacgttgaat tgagaatact gcacatacac ccccgggtgt
      301 aatcaggccc tttcaatcac tttattcgga agatgtttgt acaggaaagc tccgatacgc
      361 caccggctgt acacataggc tatttttcgt ttctttcgga ttgccttgaa tatcagttgt
      421 gcagctttat cgggcgggca aacccaaaaa accatggtgg ttcccttcat atttgtttcc
      481 atatatccgg gaatgatgtt ggtaaccgtg atgggtaatt tttcgtgacg ggccagattt
      541 ttcagtcctt ccaggtagtt catctggtaa cctttactgg cattgtaagc cacattgttg
      601 cgccatccgc gcagcccggc caccgaggtt atacccacca gatgtccggt tccctgattg
      661 cgaaacagat taaacgccca atttgaaatg agtgtaaagg ctgtgacatt caccaggttt
      721 gtagtatatt caatgtcgta atcgagtttt gtattatatt tgatcaggcc tgcactgatg
      781 accagcagat ccagtcctcc taattccctc accagttctt ccagatttgg gagtattatt
      841 tcagtattaa tcaggtcgaa aggtttcacc agataagcat cgggtttttc ggctgccagc
      901 tcgtgcagca aattttccct gcgggctgtt atgcctaccc tgtagccgtt ttctaccagt
      961 attttggcta actcacgtcc cactcccgac gaggcgccca ctacgattgc ttttttcatt
     1021 attaatgttt atattgctga taatgtaaat aataaatcaa tccagaattg ccggttaaaa
     1081 acaattaggt tctgaattga ttataatttc ttaatcattt tgatgatttc agccgttcct
     1141 gtcagtgata ggtgaaatta tttttaatgc aaatttatga aaaaatattt cgaaaactgt
     1201 atgtgaattc ctttaaagag acaaaaataa atatttatga tacccttttt gaaatatgat
     1261 accacaaaca cagattgttt tttaagcatt ttttttaatt ttgtaagcaa aataacaaga
     1321 agaagagatg atggacgaat tgaaaatagc aaaagttgat atcggacaaa ttgtaaggag
     1381 cgaatttgcg ggaaaaaaga tacccggatt ttttataaaa attctgaagt ggataggaca
     1441 cgaagaggga ctcaataaac tgtttgcgtc agctcccgga aaaaaaaacc tcgattttgt
     1501 ggatgcctgc atggattttt acaggattaa atgcaatgtg attggccttg aaaatctgcc
     1561 ttcggacggg aaacctctga tttttgtcag caatcatccg ctgggagcgc tggaggcaat
     1621 ctgtattgca catgtgattg gtcatcggta caatggaaaa attaattttt acgccaatga
     1681 aatactcaca tgggttgaac cactgaagga aaaatttata ccggtttcca aaaagggtta
     1741 tcagaacaga gaatctgtaa agaaagttca ggaattcttc cagtcagacg aacatgtggt
     1801 gatattccct gccggtgcaa catcacgcaa atacgggaat aaaataatcg atttggaatg
     1861 gcacaaaaat tttgtacaaa aatcggtgca gtatcagcgc gatgtcatac cactctactt
     1921 caaagggaag aattctactt ttttttacct gcttgagaaa ttcagggaga tgatccggtc
     1981 taaagtgaat tttgagatgc tttttctggt ggatgagata tttaagcaac ggggcaatac
     2041 atttacgctt tatatcggta agcctgtagg ctggcaaact ttcgacaaca ccaaaacacc
     2101 agggcaatgg gcacaaacgg tgaaaaaaat ggtatacaaa ctgcccgaaa atgagtgaat
     2161 aaaagtaatt attatcatga aatttgctca tttgcttaaa aactgtatat ttgcatcaaa
     2221 ataaaaaaac aagatgaaat ctgtccccaa atttctgttt ataattattg ccgtgatggt
     2281 gtctgtaatc gcaggcacta tgggcgtatc cggacagaat gcagcttcag cagaagatcg
     2341 gcaatatctg gtgaaagtgg gggatatggc tccggatttt gaaatgaaat tacccgatgg
     2401 aagtaaagtg aaactttcat cgcttcgcgg aaaaatagtg atgctgcaat ttacggccag
     2461 ttggtgcggt gtgtgtcgca aagaaatgcc tcatatcgaa aaagaaatct ggctgaaaca
     2521 taaaaacaat ccgaactttg cactatatgg tgttgaccgc gatgaaccgg cttcaaaggt
     2581 gaaacaattc gcaaaagata ccggtgtgac ataccccatt gggctcgatc cgaaggccgg
     2641 tatattcgga ctttatgcgt tgaaggaagc aggaattacc cgtaacgtga ttgtggatga
     2701 aaatggtaaa atagtgatgc ttacccgatt gtacgatgaa caggaatttg ccggaatggt
     2761 gtcgctgata gataaactgt tagctgaaat gtaagaataa taacccatta taatccaaca
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgcatcttt cccaatttgc
     2941 aggctggata ttaccgggcc tgggtttcgt gctccccatt gccatgtggc tcaccaataa
     3001 ggacgagaat gaaaatgtgg ataaacacgg aaagaatatc ctcaatttta ctatcagtta
     3061 ttttatttat gctgccattt cgggcattct ggtcattata gccataggac ttgctctgct
     3121 tgctgtgctc ggtgtagtgg ttactattgt tatcattatt gcagcagtca aagcgtataa
     3181 caatgaatac tgggattatc cgcttaccat taagtttttg aaataaaatc cccgaaatta
     3241 aataaagcaa acgtgtgggg taatgcaata acttcacacg tttggtgttt ttaaatgaaa
     3301 atcccaatcc caattaccgt gacctacata gatatacaca cccacaaacc cgaaatgcag
     3361 accggtgaac ttacaatact gaatgtgtca ttgtcggaag ccggaaaagt gctgtcgctc
     3421 agtgaaaacc gtctgttttc gctgggcatt catccgtgga atgccgataa tattttcgaa
     3481 aatgataccg aacaattgaa actttgggta gccagcaagt gggtgcggct ggtgggagaa
     3541 tgcgggctcg ataaactggc taaagcaggt tatgatattc aactgcatta tttcaaaaaa
     3601 caaattgaac tttccgaaca gtttcaaaaa ccgctgattg ttcattgtgt gggttattac
     3661 aatgaattat tcgacttgcg gatgcagctg aaacccgctc agaagtggat tattcacggc
     3721 ttccgcggta aaccccagct ggcagaacaa gccctgaaag caggctgtga tttgtcgttc
     3781 ggcgaacatt tcaatgccga aagtgtggag ataaccccca ccgaccgatt gtatgtggaa
     3841 acggacgaaa gctccctgcc cattgccgaa atataccacc ggatagccca aataaaaggg
     3901 tgtgaagcag atcaactcac tgccggtgct aagttgctga attgttaatt atgtttttct
     3961 tacattttaa ttctgtaaaa ggaaagatcc aaacgattga aattgtatag ctttgccctg
     4021 tttttttagt ataaatattt catataagac atatcatcca atgagaaagt acagtttgtt
     4081 tccactcctg tttttagcaa ctactctttt ttcacaaggc atatcacctg ttcagcagcg
     4141 caaactggtg caggctcttt cggccatttc aagtttgtat gtggatacca tcaacgatac
     4201 aaaactggtt gaatcatcca tccgcggtgc gttgaaagac ctggatccgc attcggtgta
     4261 cacccccaaa gaggaagtac agcgcatgcg cgaaccgctc gaaggaagtt ttgaaggtat
     4321 cggagtgcag tttcagatac tgaacgatac catcatggtg gttcagacta tagccggaac
     4381 gcctgccgaa aaagtgggtg tacttcccgg cgataaaatt gtgtatatca atgatgagct
     4441 ggtggccggt tcggcgtcaa aggttcagaa ttcagacgta ttcaagcgcc ttcgcggaaa
     4501 aaaaggcacc gaagtgctcg taaaaatcaa gcgtggaaac cttcccgaac tcatcgaatt
     4561 taagattatc cgcgataaaa ttcccattta cagtgtggat gccacctata tggctgacag
     4621 taaaacaggc tacatcaaaa taaacagttt tgggagtacc acattcgatg aatttaacaa
     4681 agcccttgaa aacctgaaaa aagaagggat gaaagatctg atactcagcc tgcagggcaa
     4741 tggtggcgga tttctcggca cagccataca aatagctgat gaatttcttc cggcaaaaaa
     4801 aatgatcgtt tatactcagg gaaacaatca accgcgtacc gatgcaacag ctacagtgac
     4861 cggaaatttt gaaaaaggca gattggtcgt actggtggat gaattttcag cctctgccag
     4921 cgaaattgtc agcggtgcac tgcaagactg ggaccgggcg gtgattgtgg gcgttcgtac
     4981 attcggcaaa ggattggttc agcgcgaatt tccattggta gatggttccc tgatgcggct
     5041 cactgtagcc cggtattata cacctactgg tcgcagcata cagagaccgt acaaggatgg
     5101 gacggaaagt tattacaaag agttatccaa ccgcttccga aatggtgaac tgatgcacgc
     5161 cgacagcatt cattttccgg attcgctgat ttacaaaacc ctcattaacg gacgaacagt
     5221 gtacggcggg ggaggcatta tgcccgatgt atttgttccg gtggatacca cggagtattc
     5281 cgattttcac cgctccatgg tagcaaaagg tgtggttagc cgaaccgctg tggaatacct
     5341 caatagccac cgtcaggaat taaaggaaaa atatgctgca ttccagaaat tcaaaaaaca
     5401 attcgaaacc ggggatgatt ttctgaaact tttgctggct gaagccgaaa aagaaaaaat
     5461 taaatacgat gacgtccagt ttaaccgttc gaaaagactt atcaaactgc aactgaaagc
     5521 tctcgttgcc cgtgacttgt ggaatataaa cgagtattat caggtgatgg acagcgaaaa
     5581 cgaatcgctg cagaaagcgc tggaaatact caacacaccg ggtgcatacg ataaaatact
     5641 gaccatctca aaactttact cccaacagaa ataactgatg caggagtatc tgagggcaca
     5701 ttgggtggaa atagcgggag cactgctgag tgttgtttac ctcgttcttt ccattcgcga
     5761 aaaagccgga ttgtggatat tcggctttat cagttcggct ttttatgtgt ttgttttctt
     5821 cgaaagcaag ttttatgccg atatgtcgct gcaattttac tacctggcag tgagtgtgtt
     5881 tggctggata aactggcacc ggaaaacaac cggaagtgat tcggccataa ttcctaccac
     5941 caccattgcc cgaacacagt attcagcata tatcgctgct attgtggtta cctatcttat
     6001 ttattacgtt attttgaaat attggacaga ttcaccggtt ccggtactcg attcgctggt
     6061 tggagcactc agcgttgttg ctacctggat gctggcgcgt aaaaagattg aaaactggct
     6121 cctgtggatt gcagctgatg cgctggcagc cggattattc ctttacaagg gactttatcc
     6181 cactgtggtg ttgtatgtca tttatacttc catggctgtg gtcggctatt ttcaatggaa
     6241 aaaacacctt caaattactt cttctgaaat atagggattg tctatatttt tgaattcata
     6301 ttcaggtcaa gatcaatcac atcattgccc cgttgtatga caatgtgcat cggaaatcgc
     6361 ctgctcgttt caaatatatc tcttatttcc gacacattca tctgaaatgc tttcatcccg
     6421 ttcacttcca gtatcagatc gccttcacgt acacccatta catctgccgg tgagtttttc
     6481 caaactttgt aaacgcgtgt aatgtaaaaa tgaggcatct cgcgcaggat ttcaattcca
     6541 gccacattgt acctgaattt gtctttaaac atcagattgg gtttcagata gaatgcggga
     6601 gttttaaaat cgatgatgca attgaagcgt ttcagggttt gattgcccaa tgtgccgtcg
     6661 cgggaagttt cctgttcgag gcctctggta taggccgagt ccggaaaagt aactaccgga
     6721 ttggttatgc agtgcggccc cagacacaac tgcaccgacc gggcataata tcctttgatt
     6781 tcaccgctca gtccttcacc gatatatccg tacacctttt ttttgggcat ggagctgatg
     6841 ctgctgttga agagccacga accaagttcg gctcccgtat caacaagcat ccgtacattt
     6901 cggggcacac tgtcggctcc ggtgtacagg gcatcaacat acattttatt gttgaccaca
     6961 gtagccggga tgcttgaata cttaccgggt actacgaatg tgtcactgtt gtaaaagtat
     7021 acacgtaatt tggtgtaatt gatctccact acataatcct tcaaaatgtc ggatcccaga
     7081 attccgttga ttttttttcc ggtataattg gtaaagttaa atatgttttc gggcaataac
     7141 agaactactt tattattgaa aattaaattt ccgattttca ggtcgttttt atagctgcgg
     7201 taagccctga tgccctgatt gttgccaagt ccctgtatca aggtgctgtc ggtagttgtt
     7261 ttgttcagag gcacatcatc gccttccatc agttcgttga tgattgtgtt tttcactccg
     7321 gtgtcgagaa tcatgctgag gggagtggag ttgttgatgg tgagttttat caccacataa
     7381 ctgcccgcca tttcgaaagg aacggaagtt acctttttat tgttcgga
//
LOCUS       DAOPZK010000095         7358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_12248_length_7358_cov_23.603040, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000095 DAOPZK010000000
VERSION     DAOPZK010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12248_length_7358_cov_23.603040"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /gene="glyA"
                     /locus_tag="PKH68_06625"
     CDS             complement(<1..733)
                     /gene="glyA"
                     /locus_tag="PKH68_06625"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNX12648.1"
                     /translation="MVRDTKIFDIIEREKQRQLKGIELIASENFVSEQVMEAMGSVLT
                     NKYAEGYPGKRYYGGCEVVDESEQLAIDRLKELFGAEWANVQPHSGAQANAAVFLACL
                     NPGDKFMGLNLAHGGHLSHGSPVNSSGILYHPCEYNLNEETGRVDYDQMEEVALREQP
                     KLIVGGGSAYSREWDYKRMREIADKVGAILMIDMAHPAGLIAAGLLDNPVKHAHIVTS
                     TTHKTLRGPRGGIIMMGKDFPNPWEK"
     gene            complement(757..1161)
                     /locus_tag="PKH68_06630"
     CDS             complement(757..1161)
                     /locus_tag="PKH68_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNX12649.1"
                     /translation="MKKVILFSIVAILVGCQSQKNLMLNNQWKLTELNGKNLTGENVE
                     ITLTFNTSNHQVNGNGGCNRYFGGYKLIDNTIEISNLGSTKMFCAETANFEEEYFNTL
                     KDKSNLEVTENQLIFSRQGKNILIFQKKIINK"
     gene            complement(1344..1886)
                     /locus_tag="PKH68_06635"
     CDS             complement(1344..1886)
                     /locus_tag="PKH68_06635"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNX12650.1"
                     /translation="MIYPLPAVMISCGETEEEYNILTVSWVGTICTNPPMCYISVRPE
                     RHSYEIIKRTGEFVINLTNEELAYATDWCGVKSGKNVNKFMEMKLTPVKGEMVNAPLI
                     KESPLCIECRVKEIIPLGSHDMFISEVINVQADTRYIDQKSDTFDLEKAKLIAYSHGH
                     YHKLGEEIGRFGWTVRKKKL"
     gene            complement(2130..2588)
                     /gene="pyrI"
                     /locus_tag="PKH68_06640"
     CDS             complement(2130..2588)
                     /gene="pyrI"
                     /locus_tag="PKH68_06640"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNX12651.1"
                     /translation="MESKKMQVAALRNGTVIDHIPSDKLFQVFNILDLGNCTNMITVG
                     NNLNSDKIVKKGIIKISDRFLEDNEASKIALIAPNAKVNIIREFEVVEKRPLRLPEEV
                     RDIVQCQNPACITNHQPVHTWFHVMTEENYTRLRCHYCEREVKLEDVRIR"
     gene            complement(2598..3503)
                     /gene="pyrB"
                     /locus_tag="PKH68_06645"
     CDS             complement(2598..3503)
                     /gene="pyrB"
                     /locus_tag="PKH68_06645"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNX12652.1"
                     /translation="MKNRSLVSISDYSKEEILSILDSAADFEANPNRKTLDGKVIATL
                     FFEPSTRTRLSFETAVNRLGGRIIGFSDSTTSSSVKGETLHDTIRMVSCYADAIVMRH
                     TLEGAARYASEISPVPIINAGDGANQHPSQTLLDLYSIFKTQGTLENLNICLVGDLKY
                     GRTVHSLIMAMSHFHPTFKFIAPEELKMPDEYKVFCKSHNIPFSESTELTDNFNDADI
                     LYITRVQKERFLDLIEFEKVKNVYTLKNDMLADTKPNLKILHPLPRVNEIDPDVDSNP
                     KAYYFQQAQNGLFVREAIISKVLFQ"
     gene            complement(3753..4349)
                     /locus_tag="PKH68_06650"
     CDS             complement(3753..4349)
                     /locus_tag="PKH68_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNX12653.1"
                     /translation="MPDSFKTISSLSEGIYKEKGSKFLSFAIPVKDLAEIKPILDNYR
                     KEYYDARHVCYAYMLGAERNEFRANDDGEPSGTAGRPILGQINSFELTNILVIVVRYF
                     GGILLGTGGLITAYKEAAADAIRQAQIVDITVKDELIVRFDYPLMNEVMRIIKEFDAQ
                     IITQQFEAACMIQISIPAKAAGAIHAKLEHIYGVMIEK"
     gene            complement(4349..5653)
                     /gene="murA"
                     /locus_tag="PKH68_06655"
     CDS             complement(4349..5653)
                     /gene="murA"
                     /locus_tag="PKH68_06655"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNX12654.1"
                     /translation="MSSFQIIGGQRLHGSITPQGAKNEALQVICAVLLTEQAVTISNI
                     PAILDVLNLIKLLEYLGVKVEQLNTDTYRFTAGNIDLAALETETFYRQSASLRGSVML
                     VGPLVARFGRAVIPKPGGDKIGRRRLDTHFIGIQKLGAEFNFDPDNKLYRIEAKELRG
                     CYMLLDEASVTGTANIIMTAVLAEGETTIYNAACEPYIQQLCRMLNGMGAQISGIGSN
                     LLTINGVKNLNGCNHTLLPDMIEVGSFIGMAAMTGSEITIKNVKYNELGIIPDSFRRL
                     GIKLEQKGDDIFIPQQDSYTIESFIDGSIMTIADAPWPGLTPDLLSVFLVVATKAQGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGNHLHLRAANMSSPDIRAGI
                     ALLIAAMSADGTSTIHNIDQIDRGYQDIDIRLNALGAKITRM"
     gene            complement(5679..6275)
                     /locus_tag="PKH68_06660"
     CDS             complement(5679..6275)
                     /locus_tag="PKH68_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNX12655.1"
                     /translation="MEYPTLQGKLIMPEYGRNIQQMIAHALTIEDREERTRCVNTIIN
                     IMGNLFPYLRDVNDFKHKLWDHVAIMSDFKLDIDYPYEILKQENLYSRPETIPYKNSR
                     IRYMHYGRNLEEMIERVATYPEGELKNEIIRMIANQMKKCFLTWNKEVVDDRKIFDDL
                     REMSKGKVDLSEENFKLVESRDVLQVAKKNKPKKNPSH"
     gene            complement(6354..>7358)
                     /locus_tag="PKH68_06665"
     CDS             complement(6354..>7358)
                     /locus_tag="PKH68_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNX12656.1"
                     /translation="CGSAKKTGSTAGNKIEVQAVSIAFYNLENLFDTINQSNNDEEFL
                     PEGAMKWTGYKYKAKLKNMSYVLSQLGLDITPHGPSIIAVSEIENRGVLEDLVKQPSI
                     ANRTYQIVHYDSPDRRGVDVGLIYNPRDYVVTNSKSYVLQTNDTSFHTRDQLVVSGYL
                     LNEKVHVIVAHWPSRIGGDVASEPKRVAAAGLTKSIIDSLYRVDPKAKIIFMGDLNDD
                     PYNKSTAVTLGAKRNIEDVAPNGLYNTLWRTLDSGVGSLAYNNQWNLFDQIIISAPFL
                     NKDKKTLTYWKSEVFNKRFLIQQEGSYKGTPMRTHAGGVWLNGYSDHFPTLIYLVKEK
                     "
BASE COUNT         1928 a         1583 c         1531 g         2316 t
ORIGIN      
        1 ttttttccca cggatttgga aaatccttac ccatcataat aatacctcca cgaggtccac
       61 gaagggtttt gtgtgttgtt gatgttacaa tatgagcatg ttttaccgga ttatcgagca
      121 gtccggcggc aatcagtcct gccggatgtg ccatatctat catcagtata gcgccaactt
      181 tatcagcaat ctcgcgcatg cgcttgtagt cccactcgcg tgaataagcc gagccgccgc
      241 cgacaataag tttgggttgt tcgcgcaatg ccacttcttc catctgatcg taatcgaccc
      301 gtccggtttc ttcattcagg ttgtattcgc acggatgata aagaatgccg gagctgttga
      361 caggcgaacc gtgtgaaagg tgaccaccgt gagccaggtt taatcccata aatttgtcac
      421 ctggatttag acaggccaga aatacagcag cattggcctg agcacccgag tgaggttgca
      481 cattagccca ttcggctccg aaaagttctt tcaggcggtc aatggcaagc tgttcgctct
      541 cgtccaccac ttcgcaaccg ccataataac gcttgccggg atagccttcg gcatatttat
      601 tggtaagtac ggagcccatg gcttccatta cctgttcact cacgaagttt tcggatgcaa
      661 taagttcgat tcctttcaac tggcgctgtt tttcgcgctc tataatgtca aaaattttag
      721 tgtctctaac catgattaaa atgatttgta tgttaattat ttattaatta tttttttttg
      781 aaatatgagg atgttttttc cttgtctact gaaaatcaat tgattttcag ttacttctag
      841 atttgattta tcttttaatg tattgaaata ttcttcttca aaattagctg tttctgcgca
      901 aaacattttt gtagaaccaa gattgcttat ttcaatggta ttatcaatta atttatagcc
      961 accaaaatac cggttacatc caccattccc atttacctga tgattggatg tgttgaaagt
     1021 caatgttatt tcaacatttt cacccgtcag gtttttcccg ttcaattcag ttaatttcca
     1081 ctgattattt aacatcagat tcttttgact ttgacaacca accagaattg caacaattga
     1141 gaataaaatt acttttttca tggaattata cattaatttg gtgaaaatgt taccgggaaa
     1201 acaaaaaagc aatttacatt tttcacattc gctttccaag gttcaaatat aggtcaattt
     1261 aaaaattatc attccaattt ttataaacta acaacaattt attcatgact ttatgtttta
     1321 ataatcaagt caatcaaaaa aaatcaaagt ttctttttcc tcaccgtcca cccgaatctt
     1381 ccaatttctt cacccaattt atgataatgt ccatgagaat aagcaatcag tttggctttt
     1441 tcaagatcga aagtatcaga cttttggtca atgtatcttg tatcagcctg aacatttatc
     1501 acttctgaaa taaacatatc gtgtgagcca agtgggatga tttcctttac cctgcattca
     1561 atacagaggg gtgattcttt gataagcggg gcgtttacca tttctccttt caccggcgtc
     1621 agtttcattt ccatgaattt attgacattt ttcccgcttt tcacaccgca ccagtcggtt
     1681 gcataggcaa gttcttcgtt ggtcaggtta ataacgaatt cacccgttcg tttgattatt
     1741 tcatatgaat gtctttcggg tcgaacagaa atgtagcaca tgggcggatt ggtgcaaatt
     1801 gtacctaccc agctcactgt tagaatatta tactcttctt ccgtctcgcc acacgatatc
     1861 atcactgccg gaaggggata aatcatcgtg ccgggcttcc aggaaacaaa agacttgtcc
     1921 tgccccccaa ccctcccgaa acaagtttcc cgataaatcg ggacaggcag ggacaggctc
     1981 tgaaggggga gttttataga gctaaaatca ttatttttag tgttcagaat attgtttttt
     2041 ttcatcagat aaatatattg taagtaacgc tattttcaga aaaacacttt tattccccct
     2101 ttagggggtt agggggccgt tttctttccc tatcttattc tcacatcctc cagttttact
     2161 tccctttcgc agtaatggca tctcaggcgg gtgtaatttt cttcggtcat tacatgaaac
     2221 caggtgtgaa ccggctggtg attggtgatg caggccggat tttgacattg tacaatatcg
     2281 cgcacttctt cgggaagtct gagtggtcgt ttttcaacca cttcaaattc gcgtataata
     2341 ttcactttag cattgggagc aatgagcgct attttactgg cttcgttatc ttccagaaaa
     2401 cggtctgata ttttaataat tccctttttt actattttat cgctattgag attgttccct
     2461 acagtaatca tattcgtaca attcccaaga tccaggatat tgaaaacctg aaataactta
     2521 tcggagggta tatggtctat tactgtgccg ttgcgcaggg cggctacctg catttttttt
     2581 gattccatgt ccactggtta ttgaaataag actttactta taattgcttc tctcacaaaa
     2641 agtccgtttt gagcctgttg aaaatagtaa gctttcggat tgctgtcaac atccggatct
     2701 atttcgttga cacggggcag cggatgcaga attttcagat ttggtttggt atcggcaagc
     2761 atatcgtttt ttagcgtgta tacatttttc accttttcaa attcaattaa atccagaaac
     2821 cgttcttttt gtacacgtgt aatgtacagt atatcagcat cgttgaagtt gtcggtcagt
     2881 tcggttgatt ccgaaaatgg aatattatgg cttttgcaga aaactttgta ttcatccggc
     2941 attttcaatt cttcgggagc aataaatttg aaggtaggat gaaaatgtga catggccatg
     3001 atgagagaat gcaccgtgcg tccgtatttg aggtcaccga ccaggcagat attaagattt
     3061 tcgagtgtgc cctgagtctt gaagatggag tataagtcca gcagtgtctg tgacggatgc
     3121 tgattggcac catcaccggc attgataatg ggaacgggag aaatttcgga agcataccgg
     3181 gcggcacctt ccagggtgtg gcgcatcacg atggcatcgg catagcaact caccatgcga
     3241 atggtgtcgt gcagagtttc acctttgacc gaactgctcg tagtggagtc cgaaaacccg
     3301 attatgcgtc ctccaagtcg gttaacggcc gtttcgaagc tcagacgggt gcgtgtggat
     3361 ggctcgaaaa acagggtggc aatcactttt ccgtccagtg ttttacgatt gggattggcc
     3421 tcaaaatcgg cagccgaatc gagtatcgac aggatttctt ctttgctgta atcagatatt
     3481 gaaacaagac ttcggttttt catacgttat ttgctgaaag taacagtgta attggtttaa
     3541 tttccaaaaa ataaaaaacc aacataaatc agtgacagat ttatattggc agttgacatt
     3601 ttcggggtga ataatccttg caacttgctg gttgtaagct gctcaaacag aaattatatt
     3661 tcatttccat cttcttcata ttcgctcaca aagatagaga atattttatg aaaaagatag
     3721 cgatgatcgt tgaatgttgt taaccggaaa aattattttt caatcatcac tccgtagata
     3781 tgttcaagct tggcatgtat ggcaccggcc gcttttgcgg ggattgaaat ttgaatcata
     3841 catgccgctt caaattgctg tgtgatgatt tgagcgtcaa attccttgat tattcgcatc
     3901 acttcattca tcagcggata atcaaaccgc acgatcagtt catctttcac ggttatatcc
     3961 acaatttgtg cctgcctgat ggcatcggca gcagcttcct tgtaggcggt aatcaatccg
     4021 ccggtaccga gcaaaattcc accaaaatag cgaacaacta ttaccagaat gtttgttagc
     4081 tcaaaagaat tgatttgtcc cagaatgggt cgtcctgccg ttcctgatgg ttctccgtca
     4141 tcgtttgccc gaaattcatt tctctcagca ccaagcatat aggcataaca tacatggcgg
     4201 gcatcgtaat actctttacg gtaattgtcg agtatgggct ttatttcggc taaatccttt
     4261 accggaatgg caaaagaaag gaatttactt cctttttctt tgtaaattcc ttctgataat
     4321 gatgatatgg ttttgaaact gtcgggcatt acatcctcgt tatcttcgct cccagtgcat
     4381 tcagtcgaat gtcaatatcc tgataaccgc ggtcaatctg gtcgatattg tgaatggtgc
     4441 tggtcccgtc ggcactcatg gcagctatca gcaatgctat tcccgcgcgt atatcgggtg
     4501 aactcatatt ggcagcccgc agatgcagat gattacccag accgataacg gttgcgcggt
     4561 gcggatcgca aagtataatc tgagcaccca tatctatcag tttgtcgaca aaaaacagtc
     4621 ggctttcgaa catcttctga tgtatgagca cacttccctg tgctttagtt gccacaacca
     4681 gaaaaacgct cagtaagtcg ggagtaaggc cgggccaggg agcatcggct atggtcatga
     4741 tggaaccatc aataaaagat tcgatggtgt agctgtcctg ctgagggatg aatatatcat
     4801 cacccttttg ttcgagtttg atgcccagcc ggcggaaact atccggaata atacccagct
     4861 cgttatattt tacattttta atggtgattt ccgagccggt cattgcagcc ataccaatga
     4921 aacttcccac ttcaatcata tcgggcagta aagtgtgatt acatccgttc aggtttttta
     4981 ctccattgat tgtaagcaga ttggaaccta ttccggaaat ctgcgcaccc atcccgttga
     5041 gcatccggca cagttgctga atgtatggct cacaggccgc attgtaaatg gtggtttcac
     5101 cttcggccag cacggcggtc ataataatgt tggctgtacc ggtcacagaa gcttcatcca
     5161 gcaacatata acaacccctg agttcttttg cttcaatgcg ataaagtttg ttgtcgggat
     5221 cgaaattaaa ctcagcaccg agcttctgaa tacctataaa atgtgtgtcg agtctgcgcc
     5281 ggccgatttt atcacctccc ggtttgggta ttaccgctct tccgaaacgt gcaacgaggg
     5341 gtcctaccag catcaccgaa cctctcagcg aagcactttg ccggtagaaa gtctcggttt
     5401 cgagggctgc gagatctata ttaccggccg taaaacggta agtgtcagta ttgagttgct
     5461 ccacttttac acccagatat tccagtagtt tgattaaatt cagaacatcc agaatggcag
     5521 gtatattgga gatagtgact gcctgctcgg tgagcaacac agcacaaata acttgtaatg
     5581 cttcattttt agctccctgc ggagtgatgg agccgtgcag acgctggccg ccgattattt
     5641 ggaacgagga catcgatagt tgttttttcg aacgaatatt aatgtgaggg atttttcttg
     5701 ggcttattct ttttcgcaac ctgaagaaca tcgcgcgatt ctaccagttt gaaattctct
     5761 tccgataaat ccacttttcc ttttgacatt tcgcgcaggt catcgaaaat tttccggtca
     5821 tcaactactt ctttattcca cgtaaggaaa cattttttca tctgattggc aatcatgcgg
     5881 attatttcat ttttcagttc accctccgga taggttgcca ctctttctat catttcttcc
     5941 agattacgac cataatgcat gtagcgtatt cttgagtttt tatacggtat cgtttccggg
     6001 cgggaataga gattttcctg tttgagtatt tcataaggat aatcgatatc cagtttgaaa
     6061 tcagacatga tagccacatg atcccacaac ttgtgtttga aatcattcac atcgcgcagg
     6121 tagggaaaca gatttcccat gatattaata attgtattca cacaccgggt gcgttcctct
     6181 ctgtcttcta tagtgagagc gtgggcaatc atttgctgta tattgcgacc atattcgggc
     6241 attatcagtt taccctgtaa ggttggatat tccatttgtt gtttttttta atttttataa
     6301 aagttcattc atgcaagcac tttccggtaa gtccggtaag ttgaaaaaaa tcattatttc
     6361 tctttcacca ggtatatcag cgtggggaag tggtcgctgt aaccgttgag ccaaacgcca
     6421 ccggcatggg tacgcatggg agtacctttg taggagcctt cctgctgaat aagaaatctt
     6481 ttgttgaaaa cttcagattt ccagtaggta agcgtttttt tatccttatt cagaaatggg
     6541 gcggaaatga taatctggtc gaaaagattc cattgattat tgtatgccag tgaaccaact
     6601 ccgctgtcaa gggttcgcca cagggtatta tacaaaccgt ttggtgctac atcttcaata
     6661 tttcttttag ctcccaaagt tacggccgta cttttattgt acggatcgtc gttgaggtcg
     6721 cccataaaaa tgatttttgc tttcggatca acccggtaga gcgaatctat aatggacttg
     6781 gtgaggccgg cagcagctac ccgttttggt tctgaggcta catcgccacc aatacgcgaa
     6841 ggccagtgag cgacgattac atgcactttt tcattcaaca gataaccgct taccaccaat
     6901 tggtcccgcg tatgaaacga agtgtcattg gtctgaagta cataggattt actgttggtc
     6961 acaacgtaat cgcggggatt gtaaattaaa cccacatcca caccgcggcg gtcgggactg
     7021 tcataatgca caatctgata cgtacggttg gcaatggaag gttgctttac cagatcttca
     7081 agtacccctc tgttttcaat ttccgatacg gcaattatcg aaggtccgtg cggtgtaata
     7141 tccagcccaa gctgagagag gacataggac atattcttca gtttggcctt gtatttatat
     7201 ccggtccatt tcatggcgcc ttcgggcaga aactcttcgt cattattgct ttgattaatg
     7261 gtatcgaaaa gattttccag gttgtaaaag gcaatgctaa cagcctgaac ttcaatttta
     7321 ttcccggcag tacttccggt ttttttagca gaaccgca
//
LOCUS       DAOPZK010000096         7315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_12344_length_7315_cov_23.652204, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000096 DAOPZK010000000
VERSION     DAOPZK010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12344_length_7315_cov_23.652204"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /gene="ilvC"
                     /locus_tag="PKH68_06670"
     CDS             <1..848
                     /gene="ilvC"
                     /locus_tag="PKH68_06670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNX12657.1"
                     /translation="EKAIADGWVPGETLFEIEEACEKASIIQYLLSDAAQIEVWPRIK
                     PYLTAGKALYFSHGFGITYKDRTGIVPPADVDVILVAPKGSGTSLRTMFLEGRGLNSS
                     YAIFQDATGRAFDRVVALGIGVGSGYLFETTFKREVYSDLTGERGTLMGAIQGLLLAQ
                     YEVLRANGHTPSEAFNETVEELTQSLMPLFAAKGMDWMYANCSTTAQRGALDWMTPFH
                     DATKPVFEKLYKEVACGNEAQRSIDTNSKPDYREGLEAELKALRESEMWQTGAVVRKL
                     RPENN"
     gene            complement(881..1669)
                     /locus_tag="PKH68_06675"
     CDS             complement(881..1669)
                     /locus_tag="PKH68_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HNX12658.1"
                     /translation="MKVSLIQTNIFWADKKANLINIEKELETLTGKTDLVVLPEMFTT
                     GFCIDQLELAEPMDGETVLTLIRWAKEYNLAITGSFIACENEKIYNRAFFVKPDGEIH
                     TADKRHLFSMGGEDKFFSSGDKRLIINYNSFNICILVCYDVRFPVWSRNVNNEYDLLV
                     YTASFPHARMDAWDALLRARAIENQAYVCGLNRVGVDGADISYSGHSVMLDFKGKEMA
                     GFSEYEESVKTVEIHKEPLEKFREKYAFWKDADRFEIKQNRELR"
     assembly_gap    1682..1781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1941..2492)
                     /locus_tag="PKH68_06680"
     CDS             complement(1941..2492)
                     /locus_tag="PKH68_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX12659.1"
                     /translation="MQKLSKFILRTFGWKPYATVEEPPKSVICVAPHTSNWDFIIGKL
                     FYWAMGRKAGFLMKKEWFFFPVGSLMRSMGGVPIDRSKRHSITQQMINEFENREKFHL
                     AITPEGTRSLNKNWKLGFYHIAVGAKVPIQLAVIDFKNKEMGIKEVFYPTGNENADLK
                     YIYDFYRKNGGPKSDDKFAVPEI"
     gene            2730..5042
                     /locus_tag="PKH68_06685"
     CDS             2730..5042
                     /locus_tag="PKH68_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX12660.1"
                     /translation="MMDFLNDLNDSQRQAVTYNDGPSLVIAGAGSGKTRVLTYKVAYL
                     LSKGVSPATILALTFTNKAASEMKKRIGEMMGFQTTRYLWMGTFHSIFSRILRSEAHS
                     IGYTKDFTIYDSSDSKSLIKSIIKELGLDEKVYKPGFIQSKISYAKNNLITADAYFAN
                     PDLLKSDNASRVPKTNEIYALYANRCKKANAMDFDDLLLQTNLLFRNYPEILDKYQNI
                     FSYILVDEYQDTNFAQYLIIKKLAEKHEHICVVGDDAQSIYSFRGANIDNILKFQSTY
                     KNAKIFKLERNYRSTQTIVNAANSLIDKNSEQIRKTIFSEKETGNLIRVSGVITDYEE
                     AFVVSNKVAALHADENIDYKDIAILYRTNSQSRVMEESLRKQNIPYRIYGGLSFYQRK
                     EIKDVIAYLRLVCNSNDEEALKRIINYPARGIGDTTVNKISDCARLHEVSMWDVLSDM
                     LRYNLPVNSGTANKLAQFRSMIDVFSQQVPVQDAYELADSIVKTTGMLADAMTDRTAE
                     NLSRIDNVQELLKAIHEYSEMKIKEEGAERVTLPEFLSEVSLLTDQDTDKDKDGNKVT
                     LMTIHAAKGLEFRTVFIVGLEEELFPSPFAETARELEEERRLFYVAITRAEEQCFISY
                     SKSRFKNGKTNFANPSRFLKDIDKQYLEQDEPSGYTAKPSFSGWDDDMEIERNRFRQT
                     PFNRVEKPEVAPTTFHRNLTKLGKSSDNSVPVINSSLPVGSFVKHGIFGLGKVLSTNL
                     VDGNEKAEIDFGEKGVKSLLLKFAKLEVLK"
     gene            5168..5773
                     /locus_tag="PKH68_06690"
     CDS             5168..5773
                     /locus_tag="PKH68_06690"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNX12661.1"
                     /translation="MKKHTLLLLTIALFIFNLPTLNAQIKSEEKIEKMIKQNATSLSN
                     PIYKVPCGTINPNSYLTSAVTVGKSVSQPLAYFSATSPSSHYINYSVTSTILVRGKTF
                     RLNLTANKAGNIPYAYAYAFADWNRDGSFETYLGKYIIISHKKHEPKVSIPIAIPQNA
                     ATGKTRIRLHYTPNDLSSVGADNEMNAGYIYDFTVFVIEKK"
     gene            5785..7212
                     /locus_tag="PKH68_06695"
     CDS             5785..7212
                     /locus_tag="PKH68_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNX12662.1"
                     /translation="MKFRQTHINKFSGGLLLTSVSVGLNAQTAEVPNHNKSSETPNVI
                     FILADDLGFSDIEAYGNQFISTPNINKLAKEGMSFTQLYSGTSVSAPSRACLMTGQHT
                     GHTKIRGNREISPEGQEPLDGSVKTIAQLFKNAGYVTGAFGKWGLGYPGSAAEPNKVG
                     FDKFYGYNCQRQAHSYYPAWLYDNSIKVILPANPYSQDLIHQQALQFIRDNKGKSFFG
                     YFAYSLPHASLEQPDDSILQMYKRKFCEPKAFKYSGDYTGTTIPRTQFAGMVTRLDNY
                     LGEIMIELEKQGILEKTIIIFTSDNGAHSEGGGDPDFFNTEKRLRGTKRSLYEGGIRV
                     PFIVKWKDHITPGTTNNYMGAFWDMMPTFTELLGMQAAWTQPTDGVSMLPTITGVGIQ
                     PQHDYLYWEFHEEGGRQAVRKGPWKLIRQNIKTSPTLELFNVDDDLGETRDLSVQYPE
                     KFQELKSIMDKARTPSELFNFGIKN"
BASE COUNT         2179 a         1553 c         1468 g         2015 t
ORIGIN      
        1 gggaaaaagc gatagctgac ggatgggtac cgggtgaaac actttttgaa attgaggaag
       61 catgcgaaaa ggctagcatc attcagtatc ttctctcgga tgcagctcag attgaagttt
      121 ggccacgcat caaaccttat cttaccgctg gtaaggcatt gtacttctcg catggatttg
      181 gcattaccta caaagatcgg acaggaattg taccgcctgc ggatgtggat gtgatacttg
      241 ttgcgccaaa gggttcgggt acttcattgc gtaccatgtt cctcgaaggc cgcggtctga
      301 attcctcgta tgccatcttc caggatgcta ccggacgagc ttttgaccgg gttgttgcac
      361 ttggaattgg tgttggctcg ggttatttgt tcgaaaccac gttcaaacgc gaagtgtatt
      421 ccgatttgac cggtgaacgc ggaactttaa tgggcgccat tcagggtctt ttgctggctc
      481 agtatgaagt acttcgtgct aacggtcaca ccccatccga agctttcaac gaaacggtgg
      541 aagaactgac ccaatcgctg atgccactat ttgcagccaa aggtatggac tggatgtatg
      601 ccaactgctc cacaactgct cagcgtggtg cactcgactg gatgacacct ttccacgatg
      661 ccacaaaacc ggtattcgaa aaactgtaca aggaagttgc atgcggtaat gaagctcagc
      721 gttccatcga caccaactcc aaacccgact atcgcgaagg attggaagca gaactgaaag
      781 ccctccgcga aagcgaaatg tggcaaacag gtgcagtggt tcggaaatta agacccgaaa
      841 acaattaata agaatattgt cacgaaacaa aacagggagc tcatctgagc tccctgtttt
      901 gttttatttc gaacctatcg gcatctttcc agaatgcgta tttttcccgg aatttttcca
      961 acggttcttt gtggatttca actgtcttca cactttcttc gtattcggaa aaaccggcca
     1021 tttcttttcc tttgaaatca agcatcaccg aatgacctga ataagaaata tctgcaccgt
     1081 ccactcccac cctgttcagg ccgcaaacat acgcctggtt ctcgatggct ctggcccgga
     1141 gcaaagcatc ccacgcatcc attcgggcat gaggaaaact agcggtatat acgagcaaat
     1201 catattcgtt attcacattg cgagaccaaa ccgggaaacg aacatcgtaa caaacaagga
     1261 tacagatgtt aaaactgttg taattaatga tcaaccgttt atcgcctgat gagaaaaatt
     1321 tatcttcacc acccatggag aataaatgac gcttatctgc cgtatgaatt tcgccgtcag
     1381 gtttgacgaa aaatgcccgg ttgtaaatct tttcgttctc gcaggcaata aaactccctg
     1441 taattgccag attatactct tttgcccagc ggataagtgt cagcactgtt tcaccgtcca
     1501 ttggttctgc aagttcgagt tggtcgatgc aaaaaccggt cgtaaacatt tcgggcaaaa
     1561 caaccaaatc agtttttcca gtcagtgttt ctaattcttt ttcaatattg ataagattag
     1621 cctttttgtc tgcccaaaat atatttgtct ggattaacga tactttcatc tttagtgttt
     1681 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgtctgccc aaaatatatt
     1801 tgtctggatt aacgatactt tcatctttag tgtttaacgt ttaacgttta atgtttaatg
     1861 tttggcgtct agcgctcttt gttctttgtt ctttgttctt tgttctttgc tctttgctct
     1921 ttgttctttg ctctttgttc ctaaatttcc ggtactgcaa acttatcgtc cgattttggt
     1981 ccgccgtttt tgcggtagaa atcataaatg tatttcagat ctgcattttc attgcctgta
     2041 gggtaaaaca cctcttttat gcccatctcc ttgttcttga aatcaataac tgccagttgg
     2101 ataggaactt tcgctcccac tgcaatgtga taaaatccaa gtttccagtt tttattcaaa
     2161 gagcgcgttc cttcgggggt tattgccaga tgaaattttt cgcgattttc aaattcgttg
     2221 atcatctgtt gtgtaatcga gtggcgtttt gagcgatcaa tcggcacacc tcccatcgaa
     2281 cgcattaaac taccaacagg aaagaaaaac cattcctttt tcatcagaaa accggctttg
     2341 cgacccatgg cccaataaaa caatttgccg atgataaaat cccagttgct tgtatgcggt
     2401 gccacacaaa taacactttt tgggggttct tcaacagtag cataaggctt ccagccaaag
     2461 gtcctgagga taaatttact taatttctgc atggtttctg atgttaattg taatgatttc
     2521 acaaaactaa gtcttttctt tcgaaacaca agttgttgaa aagtaatttc tgaaaattag
     2581 ctgtaattta ctaactttgc aatatctata atttggatca ataaattgcc tgataacgac
     2641 ccaatagatt tatttacaat taattgtaag gatataagca ataatttagg agtgaaaaac
     2701 gattaagttc cggtgatcaa aattaaataa tgatggattt tctcaacgat ttgaacgata
     2761 gtcagcggca agctgtaacc tacaacgacg gaccatcgct ggtaatagcc ggtgctggtt
     2821 ccgggaagac gcgtgtactc acttataaag tggcctacct gctttctaag ggcgtttcac
     2881 ccgccaccat tctggcactt acattcacca ataaggcggc ttctgaaatg aaaaaacgca
     2941 ttggtgagat gatgggtttt cagactacgc gttatctctg gatgggaact tttcattcca
     3001 ttttttcacg cattttgcgc agcgaagccc atagtatcgg atacaccaaa gattttacca
     3061 tttacgatag ttcggacagt aaaagtctga taaaaagtat cataaaagaa ttaggactgg
     3121 acgaaaaagt gtacaagccc ggttttattc agtcaaaaat atcgtatgcc aaaaataatc
     3181 tgataactgc ggatgcatat tttgccaatc ccgacttgct gaaatcggac aacgcatcac
     3241 gcgtgccgaa aacgaatgaa atttacgcat tgtatgccaa tcgctgtaaa aaggcgaatg
     3301 caatggattt cgatgattta ttgctccaaa ccaatctgct tttccgtaat tatcccgaaa
     3361 tactggacaa ataccaaaac atcttctcat acattttggt ggacgaatac caggacacca
     3421 attttgccca atacctgata atcaaaaaac tggcagaaaa gcacgaacat atttgcgtgg
     3481 tgggcgatga tgcgcagagc atttactctt tccgcggtgc caatattgac aatatcctga
     3541 aatttcaatc gacttataaa aatgctaaaa tatttaagct tgaacgaaat taccgctcca
     3601 cccaaaccat tgtaaatgcg gccaatagcc tgatagataa aaattcggaa caaatccgca
     3661 agactatttt ttcggaaaaa gaaaccggca atcttatcag ggtttcaggt gtgattaccg
     3721 attatgagga agcattcgtg gtttcaaata aggtagctgc tcttcacgcc gacgaaaata
     3781 ttgattacaa agatattgcc atactttatc gcaccaactc gcagtcgcgt gtgatggaag
     3841 agtcgctgcg caaacaaaac attccttacc gtatttacgg cggtttgtca ttctatcagc
     3901 ggaaagaaat caaggatgtg atagcttatc tgcgactggt gtgcaattcc aacgacgagg
     3961 aagccctgaa acgtattatc aattatccgg cgcgcggcat aggcgatacc actgtcaata
     4021 aaatatccga ttgtgcacga cttcacgaag taagtatgtg ggatgtgctg agcgatatgc
     4081 tgcggtataa tttacctgtc aactccggca cagccaataa actggcgcaa ttccggagta
     4141 tgattgacgt attttcgcaa caggttcctg tacaggatgc ttacgagctg gccgacagca
     4201 ttgtaaaaac taccggtatg cttgccgatg ccatgaccga ccgcactgcc gaaaatctga
     4261 gccggattga caacgtacag gaattgttga aagccattca tgaatattct gagatgaaaa
     4321 taaaggaaga aggtgccgaa cgggttacac ttcccgaatt tctgtcggaa gtttcgctgc
     4381 tcaccgacca ggataccgat aaagacaagg atggtaataa agtcacgctg atgaccattc
     4441 acgctgcaaa aggccttgaa ttcaggaccg tattcattgt cgggctggaa gaagaattgt
     4501 ttccttcgcc atttgccgag accgcccgcg aactggaaga agaacgacgg cttttttacg
     4561 tggccatcac ccgtgccgaa gagcagtgtt ttatcagcta ttccaaatca cgcttcaaaa
     4621 acggcaaaac aaactttgct aatccgagcc gttttctgaa agacattgac aaacaatatc
     4681 ttgaacagga tgagccaagc ggctatacag ccaaaccttc attttccggc tgggatgacg
     4741 acatggaaat agagcgtaac cgttttcgcc aaacgccgtt caaccgggtg gaaaaaccgg
     4801 aagttgcacc cactacattc catcgcaatc tgaccaagct cggtaagagt tccgataatt
     4861 cagtacctgt catcaactca agtctgccgg tgggttcgtt tgtgaaacat ggcattttcg
     4921 gcctgggtaa agtgctatca accaatctgg ttgacggtaa tgaaaaagca gaaattgatt
     4981 tcggagaaaa aggtgtgaaa tcgttgctgc tgaaatttgc caaactggaa gttttgaaat
     5041 aaaaatcaca ttacaaaggc gaataattca ccttgtgctt aaaaaaacgt tctgaatgtt
     5101 cataaatcaa ttcttactaa gcgtaaatta ttacatttgc atctgatact taaatttctt
     5161 atcaactatg aaaaaacaca cgttgttact gttgacaatt gctttattta ttttcaatct
     5221 gccgacgttg aatgcacaga taaaatccga agaaaagatt gaaaagatga taaaacaaaa
     5281 tgcaacttcc ctctccaatc ccatttataa agtcccgtgc ggaacaataa atccaaattc
     5341 ttatctcaca agtgctgtaa cagttgggaa gtcggtcagc caaccactgg cttatttttc
     5401 agctacttca ccatcgagcc attacattaa ttactctgtt acttctacga tattggtcag
     5461 agggaaaaca tttagactaa atctcacggc gaataaagca ggtaatatcc cttatgctta
     5521 cgcctatgct tttgctgatt ggaacaggga tggcagtttt gaaacatatc tgggaaaata
     5581 tataattatt tctcacaaaa agcatgaacc gaaagtaagc atacctattg ccattccaca
     5641 aaatgctgcc accggcaaaa caagaattcg ccttcattat acgcccaatg atctatcgtc
     5701 agtgggtgct gataatgaaa tgaatgcagg ctatatttat gattttacgg tatttgtaat
     5761 tgagaaaaaa taaatttgaa aactatgaaa ttcaggcaaa cacatatcaa taaattttcc
     5821 ggcggtttac tgctgacctc cgtgtctgtt gggttaaatg ctcaaactgc cgaagttccc
     5881 aatcacaata aatcttctga gactcccaat gtaattttta tcctggccga cgatctcggg
     5941 ttcagcgata ttgaagctta tggaaaccaa ttcatttcca cgccgaatat caacaaactt
     6001 gccaaagaag ggatgtcgtt tactcaactt tattcgggta catcggtaag tgcaccttca
     6061 agagcctgcc tgatgacagg tcaacatacc ggtcatacca aaataagagg aaacagggaa
     6121 atatcgcccg aaggtcagga accgttggac ggtagtgtaa aaaccattgc acaacttttc
     6181 aagaatgcag gatacgttac cggcgctttt ggaaaatggg gattaggcta tcccgggtca
     6241 gcagccgaac ccaacaaagt gggatttgac aaattctacg gatacaattg ccagcgacag
     6301 gctcattcct attatccggc ttggctgtat gataattcta ttaaagttat cctgccagcc
     6361 aacccctatt cacaggatct gattcatcag caggcgttgc aatttatacg cgataacaaa
     6421 ggaaaatcat tttttggata tttcgcatac tcgttaccac atgcatcact cgaacagcca
     6481 gacgacagca ttctccaaat gtataagagg aaattctgtg aaccaaaagc atttaaatat
     6541 tccggcgact acacaggaac cactattccc cgcacacaat ttgccggaat ggtgacacgc
     6601 cttgataatt acttaggcga aatcatgatc gaactggaaa aacagggtat actggaaaaa
     6661 accattatta ttttcaccag cgataatggt gcacattccg aaggcggcgg tgatcccgat
     6721 tttttcaata ccgaaaaaag actaagaggc acaaaacgct ccttatatga gggtggaatt
     6781 cgtgttcctt ttattgtgaa atggaaggat cacattactc ccggaacaac aaataactac
     6841 atgggggcat tttgggatat gatgcccacc tttaccgaat tactcgggat gcaggctgcc
     6901 tggacacaac caaccgacgg agtttcgatg ctgccaacta ttaccggtgt agggatacaa
     6961 ccgcaacatg attacctcta ctgggagttt catgaagaag gcggacgaca ggctgtaaga
     7021 aaaggtccct ggaaactaat ccgtcagaat ataaaaacat cgcctaccct ggaattattc
     7081 aacgtagatg atgatctggg tgaaaccagg gatctttcgg ttcaatatcc ggaaaaattt
     7141 caggaattga aaagcataat ggataaggcc cgtacacctt ctgaattatt caattttgga
     7201 ataaaaaatt aaatcaccaa ttttaaattt ttcgattaat aattcagaga gatatttttc
     7261 tctgaattat ttttttatct ttgtattact aaaaatacaa aaaaataata tatgg
//
LOCUS       DAOPZK010000097         7249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_12494_length_7249_cov_24.369892, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000097 DAOPZK010000000
VERSION     DAOPZK010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12494_length_7249_cov_24.369892"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..2181)
                     /locus_tag="PKH68_06700"
     CDS             complement(118..2181)
                     /locus_tag="PKH68_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12663.1"
                     /translation="MNKLKALIITCLLVAVAAAGCQSGSKGNETMKQKNFISTKTVEK
                     VSSALIDSLGQSAKFRVERGVKQVAALWVESDGNETEFTQFCRDNFINDTTKLRTLYQ
                     RLEQNFETLNGYFHQIDVKLKEPIQLTGYESTPLDMLFGGYDVTAHLDEDLYTNKVAF
                     ITALNFPFYSLEEKTKLGETWTRQQWAYARMGDRFISRIPSADLQHLSQTLTDADSYI
                     SNYNIFMGQLVDDKGQKLFPADMQLITHWGLRDELKSDYADQVNGKTKQEMIYEVMKR
                     IIDQSIPQEVINKGDYNWNPLTNKLSQNGKEITANPENNKRYEVLLNNFKANRRIDAF
                     SPHYPDAISRNFDATMEIPLKDVENLFRSLLASPQVKDVAKLIQSRLGRELRPYDIWY
                     NGFKAKGTISEDELTKITAAKYPNPKALEKDLSTILVKLGFKTDKAQQIASLVQVDPS
                     LGAGHAWGAMMKGDKAHLRTRIGAKGMDYKGYNIAVHEFGHNTEQTITLNDVDYWMLN
                     GVPNNAFTEAVAFMFQKRDLELLGIKNTNPDAEAYLALDNFWACYEIMGVALVDIAVW
                     QWMYANPDATPAQLKDAVISEAQKVWNQYYAGILGGKDEPILGIYSHMIDYPLYLSYY
                     PLGHLIDFQVEQQMKGKNFAEELQRMYAQGRIVPQIWMQRSVGNSISVEPTLKAVDEA
                     LKVVK"
     gene            2462..3124
                     /locus_tag="PKH68_06705"
     CDS             2462..3124
                     /locus_tag="PKH68_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNX12664.1"
                     /translation="MLIKVCGMIYPDNIHEVAHLHPDFMGFELFPASPRYAGNSIDLQ
                     TLEKIPRKVKKIGVFVHEDLETIIGAVCKYDLDGVQIHGADRVKVCHELKNDNLIVIK
                     AFSVMNIDNFKMTKAYSEVCDYFIFDIRKDVYGRVTDKKFNWNLLTEYRGEVPFLLKG
                     HMSVEDVHLIKHFKHPKLIGVDVNSKFESEPGVKDSVKLKSFIEEIRRKEDYGLIPPK
                     VV"
     gene            complement(3205..4161)
                     /locus_tag="PKH68_06710"
     CDS             complement(3205..4161)
                     /locus_tag="PKH68_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNX12665.1"
                     /translation="MKRTWLLFSMACLLACGFVACGPKEKDKKDIVIAVIPKVDNEIF
                     DQVKVSATEAAKELGITLTWEAPTSNSGQKQKELIENLIKYKVDGILISCNDEVFLRE
                     PINEAAKAGIKVATYDSDCPVSKRIFYIGTDNEKAGKICGETLLKLYEKNKRTPNQIL
                     VLSGGTTALNMKQRLKGFRDVISADKINGVLYSMEMADFGKDLLSLKLSENKKINGVQ
                     LLWSVPVLGGVDSVPALARYMKNGGISVFFDVSKPLLRYIKTNPNCATMKQDFHAMGY
                     NGVKNLYNAITEKPFEPEILYDVKVVDQGNAEEELKLLEKPK"
     gene            complement(4448..4669)
                     /locus_tag="PKH68_06715"
     CDS             complement(4448..4669)
                     /locus_tag="PKH68_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12666.1"
                     /translation="MNILKLLGRLGQGAFSGRRAGGNIRTWFTNRNEENERARNHAIT
                     HEVKHIPIRNGSRGEGIGMGHRNKKSSTK"
     gene            4893..5621
                     /locus_tag="PKH68_06720"
     CDS             4893..5621
                     /locus_tag="PKH68_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12667.1"
                     /translation="MKKSVSVINRTFVWTGIFSIAMGVLEAIVVVYLRQVYYPNGFMF
                     PLLFITPEMMYVEWTRETATVVMLAAVGIIAGRDNLSRFLYFLFSFAIWDLIYYAALK
                     VILDWPASLLTWDILFLIPVPWISPVLAPVICSAAMIVMSVTFMYKREMHRGFKIRMT
                     EWILTLTGAVLILLSFVEDFLMLIIQSDFDMKANSGFWERAARFVPVTFNWWLFAVGI
                     GLILTSITATAYHFRKKTIRPLKR"
     gene            complement(5870..6181)
                     /locus_tag="PKH68_06725"
     CDS             complement(5870..6181)
                     /locus_tag="PKH68_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX12668.1"
                     /translation="MLKIHELIRASGYTLKEIADAVGDTEENLTRSISGNPTMDKLEM
                     IAHILGVKVTDFLCENQEEDELYGVVIFKKKTYKIGNRQDLDKLYDDVHKGYYQVKSF
                     F"
     gene            6536..7000
                     /locus_tag="PKH68_06730"
     CDS             6536..7000
                     /locus_tag="PKH68_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12669.1"
                     /translation="METNANHPKINRKEQEAGEELEKLKYIQDHYLNSLSQDQIKQYN
                     AMTNTEKLISLAKFQEKEDQYTMEKSRHLGIKSENFSELDALIKKYLRALPDKDIKRL
                     KLHLVKVAKIVDEQIETNIRKEKENEEKKLALEIERLEKQLASKKEKLNFHL"
BASE COUNT         2051 a         1466 c         1414 g         2318 t
ORIGIN      
        1 caaggacgat ttttaacgcc gttctcactg atttttcggc tcaccggacg aggtcgattg
       61 gttcagaaat ggagtacgaa aacataaaaa acagcatgcg aaaattgaga aagtttttta
      121 tttcaccact ttcaatgctt cgtccacagc tttcagcgtt ggttccaccg aaatagaatt
      181 tcccacactc cgctgcatcc agatttgcgg aacaatgcgt ccctgtgcat acattcgctg
      241 caattcttct gcaaagtttt ttcctttcat ctgctgttct acctgaaaat caataagatg
      301 tccgagcgga taataggaca aataaagggg ataatcaatc atgtgggaat aaatgccaag
      361 gattggttca tctttaccgc ccagaatgcc tgcatagtac tggttccaca ctttctgagc
      421 ttcagagatt acagcatcct tcagctgtgc gggagtggcg tccggattag catacatcca
      481 ttgccacacc gcaatatcca ccagggccac acccattatt tcgtagcagg cccagaaatt
      541 atcgagcgcc agataagctt ccgcatcggg atttgtattt tttattccaa gcaattccaa
      601 atcgcgtttc tggaacataa acgccacggc ttcggtaaat gcattgttgg gtaccccgtt
      661 gagcatccag taatccacgt cattgagggt gatggtttgc tcggtattgt gtccgaattc
      721 atgtacagct atgttgtatc ctttgtaatc catgcctttg gctccgatac gggtgcgcag
      781 atgagcttta tctcctttca tcatggctcc ccaggcatga ccggctccaa gcgaagggtc
      841 aacctgaacg agcgatgcaa tttgttgtgc tttgtctgtc ttgaaaccaa gttttacaag
      901 tatcgttgac aaatcttttt ccagcgcttt cggattgggg tatttagcag cggtgatttt
      961 ggtcagttca tcttctgaaa ttgttccctt tgctttgaaa ccgttgtacc aaatgtcgta
     1021 cggacgaagt tcgcgaccta gtctggattg aataagtttt gccacgtcct tcacctgagg
     1081 cgatgcaagc agggagcgaa ataaattttc cacatctttc agcggaattt ccatcgtagc
     1141 atcaaaattt cgggaaatag catcgggata atgcggtgag aatgcgtcaa tccggcggtt
     1201 ggctttgaag ttgttgagga gcacttcgta acgtttatta ttttcgggat tggctgtgat
     1261 ttcctttccg ttctgcgata atttattggt taacgggttc cagttgtagt cacctttatt
     1321 gattacttcc tgcggaatgc tctgatcgat aatacgtttc atcacttcgt aaatcatctc
     1381 ctgtttggtt ttaccattca cctggtcggc ataatccgat ttcagttcgt cgcgcagccc
     1441 ccagtgggta attaattgca tatcagcggg gaacagcttt tgccctttgt catccaccaa
     1501 ttgtcccata aagatattat aattggaaat ataggaatcg gcatcggtga gagtttgcga
     1561 caaatgctgc agatcagccg aaggtatgcg tgaaatgaag cggtcgccca ttcgtgcata
     1621 agcccattgc tggcgggtcc aggtttcacc cagttttgtt ttttcttcca atgaataaaa
     1681 aggaaaattc aacgcggtga tgaatgccac tttgttggtg tagaggtctt cgtcgagatg
     1741 agccgtgaca tcatatccgc caaagagcat atccagcggc gtggattcat atccggtcaa
     1801 ctggatgggt tcttttagtt tgacatcaat ctgatgaaaa tagccgttga gcgtttcaaa
     1861 attttgctcc aaccgctgat aaagtgttct cagtttggtg gtatcgttta taaaattatc
     1921 cctgcagaat tgagtaaatt cggtttcatt accatcactt tcgacccaca atgccgccac
     1981 ctgtttcact cctctttcca ctctgaattt ggccgactga ccaagcgagt caatcagtgc
     2041 cgaactgact ttttctacag ttttcgtgct gataaaattc ttttgtttca tggtttcatt
     2101 tcctttgctg cccgactggc atcctgcagc agccactgct acaagcagac aggtaataat
     2161 caatgctttc agtttattca tagacttttt tatttaaata tttcactcct gttttttgga
     2221 tcaatgacaa agatatgcag aattttttcg accaacaatc aatcgcgcga caatctgtta
     2281 ttcattctaa tcgggtctgt ttatttgaaa ataattttta aataattttt agttgaaaca
     2341 aaacaaagaa ttaaaatgtt taaatgcaag aagcgaataa aattgtctaa ttttgagtac
     2401 cctttaggac gtcaatcagt gaactaactg tcagcaaaac tcacataaaa acatcaccat
     2461 tatgttaatt aaagtttgcg gaatgattta tccggacaat attcatgaag ttgcgcattt
     2521 acatcccgat ttcatgggat ttgaattatt tcctgcttct cccaggtacg caggaaacag
     2581 tattgacctt cagacactgg aaaaaattcc ccggaaagta aaaaaaatcg gtgtgtttgt
     2641 tcatgaagat ttagaaacca taatcggtgc ggtatgcaag tacgatttgg atggagtgca
     2701 gatacacggc gccgacaggg taaaagtctg tcatgaatta aagaacgata acctgatagt
     2761 aatcaaggct ttttcggtga tgaacattga taatttcaag atgacaaagg cttattccga
     2821 ggtatgtgat tattttatat tcgatatccg caaagacgtt tacggacggg ttacggataa
     2881 aaaattcaac tggaatttac ttaccgaata ccggggcgaa gtaccctttc tcttgaaggg
     2941 gcacatgtcg gtggaagatg ttcatctcat caaacatttc aaacacccca aactgattgg
     3001 tgtggatgtc aacagtaaat tcgaatcgga accgggagtg aaagacagtg tgaagttgaa
     3061 atcgtttatt gaggaaatac ggaggaagga agattacgga cttattcctc ccaaggtagt
     3121 gtaaatccgg ctatttcaat ttacaaaaaa ggcgttaacc tttcgggttt aacgccttta
     3181 ttttttgaaa tcggccaatg gatattattt tggtttttca agcaatttca gttcctcttc
     3241 cgcattgcct tgatctacga ccttcacatc gtagaggatt tccggttcga atggtttctc
     3301 cgtaatggca ttgtagagat ttttcacccc gttgtagccc atagcatgga agtcctgctt
     3361 catggtggcg caattcgggt tggtctttat atatcggagc agcggttttg aaacatcgaa
     3421 gaaaacagaa atgccgccat ttttcatata tcttgccagg gcaggcacac tatccactcc
     3481 ccccaacaca ggtacactcc agagtaattg cacgccatta atcttcttat tctcagacaa
     3541 tttgaggctc aacagatctt tgccgaaatc ggccatttcc atagaataaa gcaccccgtt
     3601 aattttatcg gcacttatca catcgcgaaa tcctttcagt cgttgtttca tgttgagagc
     3661 agtggtgccg ccactcaaca cgaggatctg attcggtgtc cgcttgtttt tttcataaag
     3721 tttgaggagt gtttcgccac aaattttccc ggctttttcg ttatcggttc caatatagaa
     3781 aatccgcttg ctaaccggac agtcggaatc gtaggtagca accttaattc cggcttttgc
     3841 agcttcgttg atgggttcac gcaaaaagac ttcgtcgttg cagctaatca gaattccatc
     3901 cactttgtat tttatcaggt tttctatcag ttctttttgt ttctgaccgc tgttggatgt
     3961 aggtgcttcc cacgtcagcg ttattcccaa ctctttggcg gcttcggtgg cactaacctt
     4021 aacctgatca aagatttcat tatccacttt ggggataaca gcaatcacaa tatctttttt
     4081 atccttctcc tttgggccgc aggctacgaa accacaagca aggagacatg ccattgaaaa
     4141 taaaagccaa gttcgtttca taatactgat tatttaatgt taacagaaaa ttataataac
     4201 tataaaatat tcagactgaa tgaatttttt tccgaaattt tcaccttagt ttttcttctc
     4261 ttttttatta aaacagttcc cgattgtttt ttgtttttga ttttctttca attacaaaat
     4321 aatcatttac tcctgctata aatgtttaca atacagcagg tcaacacttg atatgaattt
     4381 ctcagaaatt gattaatgat gattatttgt cagttcaatc agttctgata ttgatcgaat
     4441 atatattcta tttagtgctt gattttttat tgcggtgccc cattcctatt ccttcaccgc
     4501 gactgccatt ccggatggga atatgtttaa cttcgtgtgt aattgcatga ttacgtgctc
     4561 tttcgttttc ttcatttctg ttcgtaaacc aagtgcggat attaccacct gccctgcggc
     4621 cggagaaagc tccctgtccc aaccggccta ataattttag gatattcata ctcctgaaaa
     4681 tttataattt actctttaat gaaacggcta accgttcaat aaataaacaa ctttgacttt
     4741 attttagttg aaggaataat tattaaaatc tacatgattg acattttttt cgtttttttg
     4801 cttcattatg ttttcgtgtt caaatcaaat ctctttccaa cattcggtga gcgaatttgt
     4861 tattaaaaag agaaaaacga tgcataactg ccatgaaaaa gtcggtgagt gtaataaaca
     4921 gaacatttgt ttggactggt attttcagca tagccatggg tgtgctcgag gcgattgttg
     4981 tggtttatct tcggcaggtt tattatccca atgggtttat gtttccactt ttattcatca
     5041 ctcccgaaat gatgtatgtg gagtggacgc gtgaaacagc gactgtggtt atgcttgctg
     5101 cagtgggaat aattgccgga agagataatt tgagccgttt tctgtatttt cttttcagtt
     5161 ttgccatttg ggacctcatt tactatgcgg cactaaaagt gatactggat tggccggcca
     5221 gtctgcttac gtgggatata ctctttctga tacccgtgcc ctggataagt ccggttctcg
     5281 ctccggttat ttgctctgct gccatgatag taatgtcggt caccttcatg tacaaaaggg
     5341 aaatgcaccg cggttttaaa atccgcatga ccgaatggat actgacactt acaggagctg
     5401 tacttatact gttatcgttt gtggaggatt tcctgatgct gattattcaa tctgatttcg
     5461 atatgaaagc caactccgga ttttgggaaa gggcagcgcg attcgtaccg gttactttca
     5521 attggtggct ctttgcagtg ggaattgggc tgatattgac aagtataaca gctacagctt
     5581 atcatttcag aaaaaaaaca atcagacctt taaaaagata gcggctaatt gacattatca
     5641 cacgactttt taaaagtctg acattctgtt gcagcagtta taatccactg tgagattcag
     5701 ggttctttgc tgtcatcttt ttcattatag ttacgaacca acaaatagag tgtcagcaat
     5761 atggcacaaa agccagcaac gattacaact gtaaaattca tagtggtagc tgttgagtta
     5821 atgaaatgga aatcacaagt atgttaagtt tctcttgcac tgaacatgtt taaaagaatg
     5881 atttcacctg atagtagcct ttgtgaacat catcgtataa tttgtccaga tcttgtctgt
     5941 tgccaatttt ataggttttt ttcttgaaaa ttaccacacc atacagttca tcttcctcct
     6001 gattttcaca caggaaatcg gtcactttca cacccagtat atgcgcaatc atttccaatt
     6061 tgtccattgt aggattgccc gatatacttc ttgtcagatt ttcttccgta tcgcccacag
     6121 cgtcagctat ttctttcagc gtataaccac ttgccctgat taattcatga atttttaaca
     6181 tgactctact aattttgaat aataaaacac tcactaaaaa ctaatcaccc ctggaataaa
     6241 aatttcagtt ttcggttcat catacatcct ctgaacttac taagcattta aatatacttt
     6301 cagccaataa taaaacgtaa atgattaatg gttagtttat ttagtttagc taaaagaata
     6361 taaatccaat taaaatgaag cattttattg gattacattt ttttttctac tgttcgcgtt
     6421 ttttattccc gacggcaaaa atattatacg tattaaaaca gcaacaaatc aggagttttc
     6481 attgttttaa tactgaaaga tatcaaattt attcaccctg aaaataaatg ctattatgga
     6541 aacgaatgca aatcacccaa aaattaaccg gaaggagcag gaagccggtg aagaactgga
     6601 aaagttgaaa tacattcagg atcattatct gaattctctc tcacaggacc aaattaagca
     6661 atacaatgca atgactaaca cggaaaaact gatttcactt gcaaagtttc aggaaaagga
     6721 agaccagtac accatggaaa aatcaaggca tttgggaatt aaatccgaaa atttcagcga
     6781 actggacgcg ctgattaaaa aatacctgcg cgcactgccc gacaaagaca tcaaaagact
     6841 taaattacat ctggtaaaag ttgcaaaaat cgtcgacgaa cagattgaaa ctaatatccg
     6901 gaaagaaaag gaaaatgaag aaaaaaaact tgccctggaa attgaacgac tcgaaaaaca
     6961 gctggcaagc aaaaaagaaa aactgaactt ccatttgtag gtttctctaa gaccggtttt
     7021 cacgttgaac gggctaatgg tattataaat gatatttttt atccacactt tgaccgaaag
     7081 cgatcgtggt gtttgattgt tgtgtttttt attctaattt tgggcgaatt atcggtttct
     7141 gataccacga caaaattatt caaatgaaaa aacacgttgc tttacttttc ctgattattt
     7201 ttctgtttag ccggaatatt atttctgttt ctgccagtga gcctgactc
//
LOCUS       DAOPZK010000098         7247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_12500_length_7247_cov_20.554227, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000098 DAOPZK010000000
VERSION     DAOPZK010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12500_length_7247_cov_20.554227"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PKH68_06735"
     CDS             complement(<1..1302)
                     /locus_tag="PKH68_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNX12670.1"
                     /translation="MKNINMKAHILIVGFLALSVFSSFGANPPKQDLKLMYDTPADCW
                     EKSLPLGNGRIGMMPGGGIETDHIVLNDITMWSGSEQDALNTDAINYLPEIRKLLLEG
                     KNTEAQEIMYEHFKCKGAGSGFGNGKDVPFGCFQMLGDLYIHQQLPVGNATQKYSRSL
                     NLSDAVAATTFTQGDVQFTREYLASHADNVLVIRISADKKKSVSFELKLTRPERANIS
                     VKDNILVMEGQLNDGYNGTTGVKFLTKMKIVSKGGTISTVGNNLSLQDADEALILIST
                     STDMLDKNYLQTVDKLLTNAEKRSYSTIKKQHIKKYKEKFDRVELNLGNQDYAVTTEK
                     RLNDFRKNDDPSFAALYFQFGRYLMISGTNEQSLPLNLQGLWSNTVQTPWNGDYHLNI
                     NLQMNYWLAEVCNLSDLHKPLIEFTKSLVPSGTATAKTYYGA"
     gene            complement(1435..3279)
                     /locus_tag="PKH68_06740"
     CDS             complement(1435..3279)
                     /locus_tag="PKH68_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNX12671.1"
                     /translation="MKKHIYLPVILLLALSFSCVSTRTTIPVALKPDTKENSSPAVVK
                     FIEQLQSRKTATPITVKFPKGRYDFYEDGAFVREYYISNHDQDNPKKVAFALENLQNL
                     TIDGQGSEFVFHGRMIPFALLKGKNITLKNFSIDFEIPALRQLRILEINKDQDELLAE
                     IYPSGNYRIDAEKLIIKGEGYELTPESSMAFRSDKRLTYRRQDVGFNPKSVAEVSPNV
                     FRIKGWHQVKATSPGERYVLRSYYRPTPGIFISECTNTVLENVKVHYAEGMGLVAQMS
                     ENITLDRFSVCLKGDSDPRFFTTQADATHFSACKGLIISKNGLYEGMADDAINIHGTY
                     LRIVKRLNNNTLRAKYMHPQAWGFKWGEPGDTVQFVESEKMEIVGNHINILKSIKATD
                     KPTEFGAKEFEIVFDNPLPEVISENGKFGVENLSWTPKVIFSDNIIRNNRARGALFST
                     PRRVICENNLFDHTHGTAILLCGDCNGWFETGACKEVIIRNNKFVNALTANYQFTNAI
                     ISIYPEIPDLKNQKKYFHSGIVIENNEFETFDRPLLYAKSTNGLIFRNNTVKYNTEFE
                     PFHWNKYMFFFEKVKNIQLLNNQFEKGIDPEKDIRTELSEPNSVILQK"
     gene            complement(3575..>3934)
                     /locus_tag="PKH68_06745"
     CDS             complement(3575..>3934)
                     /locus_tag="PKH68_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12672.1"
                     /translation="TGNQYTVTGLTGNQQYFYRLRAVLGSSTSPYSGLIGVTTVSTGT
                     APVHHLSYKIGTSPAGIHIYDLKGSEIVKLYSVTGSLQTNQRALSGTLTLPVQTRGIF
                     ILQISDGRNTETYKIVR"
     assembly_gap    3935..4034
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4035..5932)
                     /locus_tag="PKH68_06750"
     CDS             complement(<4035..5932)
                     /locus_tag="PKH68_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNX12673.1"
                     /translation="MRKQVLLTILISTSFLAQAAIPTGYYHFALGKKKAELKTTLHEI
                     SAPLTVLDYGSGPGFTWQGFYATDRNADNTVRDMYSNNVRSFNGYNSIADMAIEHSFP
                     KSWWGAYENMAYKDLFHLYPADAYTNGIKNNLPLGEVTGTPMLDNGKSKVGKNGFGTA
                     YSDNSFEPADEFKGDFARSYLYISTIYEDLAPLWNSPMLTNTVYPVWRPWAIDLLMKW
                     HNNDPVSQKELDRNDSIYVIQGNRNPFIDFPELTSYIWGTDSTKAFTYAEETGAFLVA
                     PRRKFKLDFGVILLNVTKSLNFNIEGVNITSPLTVSFLRNSPSLSVSNTTISAAEAQA
                     GFNLQVNYLPTITGTTVDTLLIQGGGLTEITRIPVRGVATSEFIAMEPADITPVSGTL
                     HWLEDPLATNYTVSLYQGDLKAGNLIISGYYEGTSFDKAIELYNGTGATVDLSKYSLK
                     KQSNGAGEFETTLKLSGTLQNNQTYVIVNTNSTNTALTAKANILKDSITSFNGNDAVA
                     LFRNGVQIDVVGKLNGGAPYVWGLDKILKRKPDITHPNSNFDLTEWNEYPFSQLEKIG
                     THTINFASASNYIFEDKPVGIVNQLPVDNLNPNENYTYSVTSYRSGVVAPSVNTQRLR
                     TTGLETPVG"
     gene            complement(6069..6941)
                     /locus_tag="PKH68_06755"
     CDS             complement(6069..6941)
                     /locus_tag="PKH68_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNX12674.1"
                     /translation="MKTAIIQQTNTADQAANVSKLEKNIREVASQGAELIVLQELHNG
                     LYFCQTEDVSVFDQAETIPGPSTKRFGKLAAELGIVIVLSLFEKRAAGLYHNTAVVLE
                     KDGTIAGMYRKMHIPDDPAYYEKFYFTPGDLGFEPIQTSVGKLGVLVCWDQWYPEAAR
                     LMALAGAEVLIYPTAIGWESSDTNEEKNRQRDAWITIQRSHAVANGIPVIACNRVGYE
                     ADPSGITKGIQFWGSSFMAGQQGEILVKTSDTKEENVVFDIDINRTETVRRWWPFFRD
                     RRIDAYQDITKRWK"
     gene            complement(6990..>7247)
                     /locus_tag="PKH68_06760"
     CDS             complement(6990..>7247)
                     /locus_tag="PKH68_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNX12675.1"
                     /translation="DRLPATYANFLIINDAVLMPTYNSPKDEIARGQLQKAFPDREII
                     GIDCTALIKQHGSLHCVTMQYGAFPNPSKGGEFGGHDLKSL"
BASE COUNT         1870 a         1517 c         1552 g         2208 t
ORIGIN      
        1 ggctccgtag taggttttgg cagtggcagt tcccgatggc accagggatt tggtaaactc
       61 aatgagtggt ttgtgcaagt ccgaaagatt gcagacttcc gccagccaat agttcatctg
      121 aagattaata ttcaggtgat aatcaccatt ccagggtgtt tgaactgtgt ttgaccacaa
      181 accctgcaag ttgagtggaa gtgattgttc attcgttccg ctaatcatca ggtatcggcc
      241 aaactgaaaa taaagggctg caaatgacgg atcgtcgttt ttccggaaat cattcaagcg
      301 tttttcggta gtaaccgcgt aatcttgatt gcccaaattt aattccaccc gatcgaattt
      361 ttccttatat tttttgatat gctgtttttt tatcgtcgaa taagaacgct tctcagcatt
      421 agtaagcaat ttatctactg tttgaagata attcttatcc agcatatcgg tagaagtgga
      481 tatcaaaatc agggcctcgt cagcatcctg caacgacaga ttatttccta ccgttgagat
      541 cgttccacct ttagacacaa ttttcatctt tgtcagaaat ttcactccgg tggttccgtt
      601 gtagccgtcg ttgagctgac cttccattac caggatatta tctttcaccg aaatatttgc
      661 acgttcgggg cgagtaagtt ttaattcaaa ggaaacggat tttttcttat cagccgaaat
      721 tcgtattacc agcacattat cggcgtgcga ggccaggtat tcgcgtgtga attgtacatc
      781 gccctgtgta aatgtggtag cagccacagc atccgacaaa ttcaaactgc gcgagtattt
      841 ttgagtagca tttccgacgg gtaattgctg atggatgtac aaatcgccca acatctgaaa
      901 acagccaaaa ggtacatcct tgccattgcc aaatccagag cctgctcctt tgcatttaaa
      961 atgctcgtac ataatttcct gtgcttctgt attttttcct tccagcagca gtttacgaat
     1021 ttcaggtaaa tagtttattg catcggtatt gagcgcatcc tgctcactac ccgaccacat
     1081 agtaatatcg ttcagcacaa tatgatccgt ttcaatgccg ccaccgggca tcatccctat
     1141 tcgtccgtta ccgagtggaa ggcttttttc ccaacaatcg gccggggtat cgtacatcaa
     1201 tttcaaatcc tgttttggcg gattggcacc gaaactgcta aaaacggaaa gtgctaaaaa
     1261 accgacaatt aaaatatgtg ctttcatgtt tatatttttc atatcagccc cctaaccccc
     1321 taaaggggga atattgagcg tctatcattt gttaagagtt atttaatcat gccattgcgt
     1381 gtccaatttt attttgttgg atatgggtgt ttcatctata ctttttttcg aaatttattt
     1441 ctgcagaatt acactgtttg gttctgataa ctcagttcga atatcctttt ccgggtcaat
     1501 gcctttttca aattggttat tcaataactg aatatttttc actttttcaa agaaaaacat
     1561 atatttattc cagtggaacg gttcaaactc ggtgttgtac ttgactgtat tatttctgaa
     1621 aatcagtccg ttggtggatt tggcatacag caacggtcga tcaaacgttt cgaattcatt
     1681 attttcaatc actattccgg aatggaagta ttttttttga tttttcaaat ccggaatttc
     1741 gggataaatg gatatgatgg cattggtgaa ctgataattg gctgtgagtg cattcacaaa
     1801 tttgttattt ctgataatca cctctttaca ggcaccggtt tcgaaccagc cgttgcagtc
     1861 gccacaaagc aggatagcag tgccgtgggt atggtcgaag aggttgtttt cgcatatcac
     1921 acgtctggga gtactgaata atgcaccccg ggcacggttg tttctgatta tattatccga
     1981 gaaaatcacc ttgggtgtcc aactcaggtt ctccacgccg aattttccgt tttcagaaat
     2041 tacttcgggc agcggattat caaagacaat ttcaaactct tttgctccaa attctgtggg
     2101 tttgtctgta gctttaattg atttcagtat attgatatga ttgccaacga tttccatttt
     2161 ctcggattcc acaaattgca cggtatcgcc gggttcaccc catttgaaac cccaggcttg
     2221 tggatgcatg tactttgccc gtaatgtatt attatttaaa cgtttaacaa ttcgcaaata
     2281 ggtgccatgt atattgattg catcgtcggc cattccttca tacagtccgt ttttggaaat
     2341 aatcagtcct ttacaggccg aaaaatgagt agcatcggcc tgtgtggtga aaaatctcgg
     2401 gtcgctgtcg cctttgaggc aaaccgaaaa tcggtccagt gtaatatttt cgctcatttg
     2461 ggctaccagt cccatgcctt cggcatagtg aacttttacg ttttccaaaa cggtattcgt
     2521 gcattcgctg ataaaaatac cgggagtggg gcgatagtac gacctcaaca catatcgttc
     2581 gccgggcgat gtcgccttca cctgatgcca tccttttatt ctaaacacat tgggtgatac
     2641 ttcggcaacc gatttcggat taaaaccgac atcctgacgc ctgtacgtga ggcgcttgtc
     2701 ggatcggaat gccattgaac tttcaggtgt aagttcgtag ccttctcctt ttataattaa
     2761 tttttcagca tcgatgcgat aatttcccga cggataaatt tcggcaagca gttcatcctg
     2821 gtctttgttt atttccagaa tacgaagctg acgaagcgcc ggaatttcaa agtcgatgga
     2881 aaagtttttc agcgttatgt tttttccctt caacagggca aaggggatca tcctgccgtg
     2941 aaatacgaat tccgatcctt gtccgtcgat ggtgaggttt tgcagatttt caagtgcaaa
     3001 ggccactttt ttgggattat cctgatcatg gttggagatg taatactccc ggacaaaagc
     3061 tccgtcttcg tagaaatcat aacggccttt tggaaattta accgtgattg gcgtagctgt
     3121 tttacgcgat tgcagctgtt ctatgaattt tactacagcc ggggaagagt tttctttagt
     3181 gtcgggtttt agagcgacgg gaatagttgt acgtgttgac acacaactga aactaagtgc
     3241 caataataaa ataaccggaa gatagatgtg ttttttcata gagatttatt gttgagatga
     3301 taaggggttg acaaaagtaa agataaaaaa gcactttcgg aaatccgaaa tggaaatggc
     3361 ctttatatag gatgaatttt tcaagctaat caacaaacaa aaacaatcag aattactgca
     3421 aaggaaatct tatttttcac aaagtcatct tctattctaa aaacagaatt caggattaaa
     3481 tcctgaattc cgggaaagat atttatttat aataagtttc agatccttct tatttccact
     3541 gtaatataat atcggtaagt caattttaaa ttatttatct gacaatttta taagtttcag
     3601 tattacgacc atccgatatt tgcaaaatga aaattccgcg ggtctgtacc ggaagtgtca
     3661 gtgtacccga aagtgccctt tgattagtct gcaaagaacc ggtaacggag taaagcttaa
     3721 ctatttctga acctttcaga tcataaatat gaataccagc gggcgatgtc ccaattttat
     3781 agctgaggtg atgaaccggc gctgttccgg tactaacggt agtaacccca atcagtccgg
     3841 agtacgggga tgtggaactt cccaaaacgg cacgtagtcg gtaaaaatac tgctgatttc
     3901 cggttaatcc ggtaacggta tattgatttc cggtnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnncccacg ggagtttcca gaccggttgt tcgcagtcgc tgggtattga
     4081 ccgagggtgc taccaccccg ctgcgatacg aagtgacgct gtaggtgtag ttttcgttgg
     4141 gatttaagtt gtcaacgggc aattggttga ctattcccac cggtttatcc tcgaaaatat
     4201 aattactcgc cgatgcaaag tttatggtgt gtgtaccgat tttttccagt tgtgagaaag
     4261 gatattcgtt ccattcggtc agatcaaaat tactgtttgg gtgtgtaata tccggttttc
     4321 ttttaagaat tttgtccaaa ccccagacat aaggagctcc accattcaac tttccaacca
     4381 catcaatctg tactccgttt cggaataagg caactgcatc atttccattg aaactggtta
     4441 ttgaatcttt caaaatatta gcttttgctg ttagtgccgt attagtagag ttagtattca
     4501 caattacata cgtctggtta ttctgtaatg ttcctgaaag tttgagtgtg gtttcaaatt
     4561 cgccggctcc gttggattgc tttttcagcg aatatttcga taaatcaacg gttgcacccg
     4621 ttccattata aagttcaatg gctttatcaa aggatgtacc ttcataatat cctgaaataa
     4681 tcagatttcc ggcttttaaa tcgccctgat agagggaaac ggtgtaattc gttgcaagcg
     4741 gatcttcgag ccagtgaagg gtaccgctta ccggtgtaat atcggcaggc tccatggcta
     4801 taaattcgga agtggcaact ccccggaccg gaatacgggt aatttcggtc agtccgccgc
     4861 cctgaataag caacgtgtca acggttgttc cggttatcgt tggtaaataa ttaacctgca
     4921 aattaaaacc ggcctgtgct tcagctgccg aaatggttgt gttcgaaaca gacaacgatg
     4981 gactgtttct taaaaacgat acagtaagcg gagatgtgat atttacccct tcgatgttga
     5041 aattgagtga tttagtgaca ttgagcaata ttaccccaaa atcgagtttg aatttacggc
     5101 gcggtgccac cagaaatgcc ccggtctctt cggcataggt aaatgctttg gtgctgtccg
     5161 ttccccagat atacgaagtc agttcaggga aatcaataaa cggattacgg ttgccctgaa
     5221 tgacgtaaat gctgtcgtta cggtcaagtt ctttctggct tacggggtcg ttattgtgcc
     5281 atttcatcag caaatcaata gcccagggtc gccatacggg ataaaccgtg ttggtaagca
     5341 tgggtgaatt ccagagggga gccaggtctt cgtagatggt ggaaatatag aggtacgaac
     5401 gtgcaaaatc gcctttgaat tcatctgccg gttcaaaact attgtccgaa taagccgtgc
     5461 caaagccatt tttacccact tttgatttcc cattgtcgag catgggtgtg ccggtaactt
     5521 cccccagtgg aagattattt ttaatgccgt tggtgtatgc gtcggccggg taaaggtgaa
     5581 aaaggtcctt gtaagccata ttttcatacg caccccacca gcttttggga aatgagtgct
     5641 ctatggccat atcggctata gaattgtatc cgttgaaact acgtacatta ttggaataca
     5701 tatcgcgcac cgtattatcc gcattgcggt cggtggcata aaaaccctgc catgtaaagc
     5761 ccggaccgct gccgtaatca agtactgtga gcggcgctga aatttcatgc agcgttgttt
     5821 tcagttccgc ttttttcttt ccaagggcaa aatgataata acccgttgga atggctgcct
     5881 gtgctaaaaa agacgtactt attaatattg taagcagaac ctgctttcgc attacattca
     5941 ttttttaata tttttggaat atatatttgg caaatgtatc gtattttgaa ttcataacaa
     6001 aacaacaaac tctttttatc attctttgac aaaaagagtt gttgaagtgt aaccggctga
     6061 tttttatttt atttccacct tttggtgatg tcctggtagg catctattcg tctgtcgcgg
     6121 aagaagggcc accagcgcct cacagtttcg gtacgattta tatcaatatc aaaaacaaca
     6181 ttctcttcct tcgtatcaga ggtctttact aatatttcgc cctgctgacc ggccatgaaa
     6241 ctgcttcccc aaaactggat gcctttggta atgcccgacg ggtcggcctc ataaccaacc
     6301 cggttgcagg caatgacagg aattccgtta gccaccgcat gtgagcgctg aatagttatc
     6361 cacgcatcac gctgacgatt tttttcttca ttagtatctg aactctccca cccgatggct
     6421 gtgggataaa tgagcacttc ggctccggca agcgccatca gccgggcagc ttcggggtac
     6481 cactgatccc agcaaacaag cactcccagt ttcccgactg aagtctgaat gggttcaaaa
     6541 cccaggtcac cgggagtaaa atagaacttt tcgtagtaag ccggatcgtc ggggatgtgc
     6601 attttgcggt acattccggc aatggttcca tctttctcga gcaccacagc agtattgtga
     6661 tacagcccgg ctgctctttt ttcgaataat gacaacacga tgacaattcc aagctctgct
     6721 gccagttttc cgaacctttt ggtggatggt ccgggaatgg tttcagcctg gtcaaagacc
     6781 gacacgtctt cggtctggca aaaatacaat ccattgtgaa gttcctgaag cacgattagt
     6841 tcagcgccct gagaagccac ttcacggatg tttttttcga gtttggacac attcgctgcc
     6901 tggtccgctg tattggtttg ttggatgata gctgttttca tatctgcccc ctaaccccct
     6961 aaagggggaa tttatcaatg ttctaaattt tataaacttt tcaaatcgtg cccaccaaac
     7021 tcccctcctt tggaggggtt ggggaaggct ccgtattgca ttgtgacaca atgcagcgag
     7081 ccgtgttgtt ttatcagtgc tgtacagtca atgccaatga tttcgcggtc agggaatgct
     7141 ttttgtaatt gtccgcgggc tatttcatct tttggagaat tataggttgg catcagcacg
     7201 gcgtcgttga ttatcagaaa gttggcgtaa gttgccggaa gtcggtc
//
LOCUS       DAOPZK010000099         6912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_13370_length_6912_cov_20.235672, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000099 DAOPZK010000000
VERSION     DAOPZK010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13370_length_6912_cov_20.235672"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="PKH68_06765"
     CDS             complement(<1..1958)
                     /locus_tag="PKH68_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX12676.1"
                     /translation="MKKIFYTLLLLACGQFSFGQWNTDRIMNIGKNALQFEDYVLSIQ
                     YFNQVIKIKPYLAEPYIYRGIAKISLGDYTGAEQDCTEAIERNPFVPQAYYARGFARK
                     NINKFQEAIVDFNKALEFSPENTDIIANRGEVREKNNDPEGAIDDLNLYRKLNPKARG
                     IDYEIGRIQLAMKDTVAALVSLDKFIISDSTNATGYSIRALIKMLQKDEKSAFEDYNK
                     AVAYKSSYSGDYINRGILNVQRNKFNQALSDYNEAIRLDPKSTLAYYNRGLLRANLGD
                     SNNAIGDLDKVVAADSTNYEARLQKAYLELKVGDMTSAIRDFNVILKKYPFFVPAYLG
                     IADAKQGLGRKKEANQYRNLASEIVNNKDYYKRKQNLVAKNQIAKETPVNELIKPVNL
                     VEKFTANNAGERKYENKYGEGVRGEIQNSVANLKDERNFELTYNAPNAETSRTNTYHP
                     LISKFNGENTSMSSLKIANDMVVQSAEQINTHFAAINRLTEQLKNTADNSELYFFRAL
                     EFSLVQDYTSAIDDLNKAIELRSNFALAYFTRANLRFKQLEYQSINQSEYTQETKNKV
                     SVVPVGDENTKIQYELILRDYDKTIDLTPDFSFAYFNKGNLYRSMKNFKSAVSSYSKA
                     IEIDPDFAESYYNRGISHLYLNDKINAT"
     gene            complement(2002..4197)
                     /locus_tag="PKH68_06770"
     CDS             complement(2002..4197)
                     /locus_tag="PKH68_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNX12677.1"
                     /translation="MFILISIGWLGYLPDIKELQNPINKSATEIYSSDMVLLGRYSYA
                     KENRVPINYNDIDKDVINALIATEDVRFYKHSGIDGKALIRVFFGLFTRSNTGGGSTI
                     TQQLSKLLYSPSASNIFKRALQKPIEWVIAVNLERMYSKEEIIAMYLNQFDFLNNAVG
                     IKSAAHVYFNTTADKLKIEEAATLIGMCKNPSYFNPVRHKERTIGRRNVVFDQMRKAD
                     IITRQQQDSLSKLPLKLNYMRVDHKEGLATYYREYLRQMLMAGEPQRSDYADWQKQKY
                     LDDKWAWENNPLYGFCKKNKKPDGSNYNIYTDGLKIYTTIDSRMQRYAEEAVDEHMQR
                     LQKSFFSEKRGRSYAPFSRDLTSQEIDDIMTRAMRQSERYRVLKEAGMSKAEITKSFN
                     TKTDMRLFSYNGMIDTTMTPMDSIRYHKHFLRCGFMSMDPGNGYVKAYVGGPNITAFQ
                     YDMVSTGRRQIGSTVKPYLYTLAMEEGMSPCDRVVCAPITIQTSSGPWTPKNGSNARL
                     GEMVTLRWGLANSNNWISARLMNQYTPETLVKLMQSFGIRGHLDPVVSLALGPCEVSV
                     SEMVDAYTAFPSKGIRVDPLYVTRIEDSNGNVIGTFVPKMQEIFSETTAYKMIYMLRS
                     VVDEGTGGRIRRVYGLTMPVGGKTGTTQNNSDGWFMGFTPKLVSGVWVGGEERSIHFD
                     GMAQGQGASMALPIWAIYMKKVLANPQLGYRSTDQFDVPDDFNPNEACE"
     gene            complement(4394..5527)
                     /gene="hemW"
                     /locus_tag="PKH68_06775"
     CDS             complement(4394..5527)
                     /gene="hemW"
                     /locus_tag="PKH68_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNX12678.1"
                     /translation="MAGIYIHIPFCKSKCSYCDFFSATDYNQQIELLAAMKKELFLQK
                     EYLKKELVSTIYFGGGTPSTLSAENILDIINSINTHFQLAENVEITLEANPDDLNISY
                     LQHLRKNGINRLSIGIQSFDNEQLTAIRRRHTAETAVRAIEDAQTCGFDNISIDLIYG
                     LPGQSLQSWKYQIDKALTLGVQHISAYGLTYEKGTLLWHQLQRGEIKQVDDETMNAMY
                     QYLVKVCVENGFEHYEISNFALPNRRSRHNSAYWHQQPYLGIGPAAHSYNLISRQWNV
                     ASIRQYCDKINSGIQWYETEILTEQDKYNDLIMVSLRTSEGIELKQVETIFGNEKAEY
                     CLISAKKYLNEKLLYQNNGFLRLTPDGILISDQIIADLMLCEE"
     gene            complement(5533..>6912)
                     /locus_tag="PKH68_06780"
     CDS             complement(5533..>6912)
                     /locus_tag="PKH68_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12679.1"
                     /translation="YIRGGMYMLSTSLSITKSGNSTAFINIWAYPNETPVLDFRNQPY
                     NSNNAGVKLSGSYLFVKGITIQGAGDNGMIVTGSNNRIEKCIFRWNCDSGLQLKTGSS
                     NLIINCDSYENFDYKTGGTAIPDYGGNADGFADKQYTNAGTNSYIGCRSWLNSDDGWD
                     HYEKTGNTTCDNCWCYANGPASYDMTDHIRFKTDSATWFYQFKNTSGRYIITNYGNGN
                     GFKLGGNYTAHNATLHHCVSVKNKVKGFDQNNNNGTMTVYNCTGYLNDTNYGFSNSSF
                     GTLVIKNCASLSSTSSNKFACKVVTQEYNSWNSGFSCTAADFASLDYTQLLTDRQADG
                     SLPEISLLHLVSTSALIDKGINLGYNYFGNAPDLGAFEFNTVSSVNLPNAVADISTYY
                     SILNKQIIVRGSTEKVEIYNISGKKMYDNAIITDNQTIATPDWAKGIYILKAFTEKGN
                     LSVFKILIP"
BASE COUNT         1830 a         1456 c         1412 g         2214 t
ORIGIN      
        1 ctggtggcat ttattttatc attcagatac aaatgggaaa ttccccggtt gtaataggat
       61 tctgcaaagt caggatcaat ttcaatggct ttggaatagg aggagactgc cgatttgaaa
      121 tttttcatcg atctgtaaag attaccctta ttaaaatacg caaaagagaa atcgggggtt
      181 aagtcaatgg ttttgtcgta atcacgcaaa atcagctcgt actgaatttt ggtattttcg
      241 tcaccgaccg gaactacact caccttattt ttggtctcct gagtatattc cgattgattt
      301 atggattgat attccaattg tttgaacctc aaattggcac gtgtgaaata tgccaatgca
      361 aaatttgacc gtagctctat agctttgttc agatcgtcaa tggcactcgt ataatcctga
      421 accaacgaaa attccagtgc gcggaaaaaa tagagttcag agttgtctgc ggtattttta
      481 agttgttccg tcagcctgtt gatagctgca aagtgggtgt ttatttgttc tgctgactgg
      541 acaaccatat cattcgcaat tttgagtgac gacatggagg tgttttcacc attgaatttt
      601 gaaatcaggg gatgataagt atttgttctg cttgtttcag catttggggc gttataagtc
      661 agttcaaaat ttcgctcatc tttaagattt gcaacgctat tctgtatctc accccgaacc
      721 ccttcgccgt atttgttttc atattttcgc tcaccggcat tgttagctgt aaatttttca
      781 accagattga ccggcttaat cagttcattg acaggagttt ctttggctat ctgatttttg
      841 gcaaccagat tttgtttacg tttatagtaa tctttattgt taacgatttc tgaagccaga
      901 tttcggtact gattggcttc ctttttcctt cccagacctt gttttgcatc ggctatacct
      961 aaataggcag gtacaaaaaa gggatatttt ttgagtataa cgttaaaatc gcggatggct
     1021 gaggtcatgt cacccacttt aagttccaga taggctttct gaaggcgtgc ttcataattg
     1081 gtgctgtcgg ctgcgaccac tttatcgaga tcaccgatgg cattattgct gtcacccaga
     1141 ttggcacgca acaatccgcg attgtaataa gccagagtac ttttgggatc aagcctgata
     1201 gcttcgttgt aatcactcag tgcctgattg aatttattgc gctgtacatt gagaattccc
     1261 cgattaatat agtcgccgga ataggacgat ttataagcta cagctttatt ataatcctca
     1321 aatgcacttt tttcatcttt ttgcagcatt tttatcagtg ccctgatgct gtaacccgtg
     1381 gcattggtac tgtctgaaat gataaatttg tcgagcgata ccaacgcagc cactgtgtct
     1441 ttcatggcca actgaatgcg tccgatttca taatcgatac cacgggcttt gggattcagt
     1501 tttcgataca ggttcaaatc gtcaattgca ccttcggggt cgttattttt ttctcgtact
     1561 tcgccccggt tggcaataat atcggtattt tcggggctaa attccagtgc tttattgaaa
     1621 tcaacgatgg cttcctgaaa tttattgata ttttttcggg caaaaccccg tgcataatag
     1681 gcttgtggaa caaaaggatt tctttcaatc gcctccgtac agtcctgttc ggctccggta
     1741 tagtcgccta gactgatttt ggcaataccc cgatagatgt agggttcggc cagatatggt
     1801 tttatcttta tcacctgatt gaaatattga atagagagga cataatcctc aaactgaagt
     1861 gcatttttcc caatattcat tattcggtca gtattccact gaccgaatga aaactgacca
     1921 catgccagca acaggagggt ataaaatatc tttttcaatc aatagttttt tttcaggatg
     1981 gattattctt tttttaccaa cttattcgca ggcttcattg gggttaaaat catcgggcac
     2041 atcaaactgg tcggtggatc ggtaccctaa ttgtggattg gcgagtactt ttttcatata
     2101 aatagcccag ataggcaatg ccatacttgc gccctgtccc tgtgccattc cgtcgaagtg
     2161 aatggagcgt tcttcgccac ccacccaaac gcctgaaaca agtttgggag taaatcccat
     2221 aaaccatcca tcggagttat tttgtgttgt tccggtttta cctcccacgg gcatcgtaag
     2281 tccgtaaacc cgccgaatac gccctccggt accttcgtca accacacttc gcagcatgta
     2341 tatcattttg taagcggtag tttcgctgaa tatttcctgc attttgggta caaaggttcc
     2401 aatcacattt ccattggagt cctctatacg ggtaacgtac aacggatcta cgcgaatacc
     2461 tttggatgga aaggcagtat aggcatccac catttcggac actgacactt cgcacggacc
     2521 cagagccagc gaaacaacgg gatcgagatg accgcgaata ccgaatgact gcatcagttt
     2581 aaccagtgtt tcgggtgtat attgattcat aagacgagcc gaaatccagt tgttggagtt
     2641 tgccaatccc caccgcaaag tgaccatttc gcctaatcgg gcattcgatc cgtttttggg
     2701 tgtccaggga cccgatgaag tttgtatggt gatgggagca cacaccactc ggtcgcaggg
     2761 cgacatgcct tcttccatag ccagtgtgta gaggtagggc ttcacggtag aaccaatctg
     2821 acggcgtccg gtactaacca tatcatactg gaaggcagta atatttggtc cgcccacata
     2881 ggctttcaca tatccgttgc cggggtccat cgacataaat ccacatcgca gaaaatgctt
     2941 gtggtaacgg atggaatcca tgggagtcat cgtagtgtca atcataccgt tataagagaa
     3001 aagcctcatg tccgtttttg tgttgaatga tttggtgatt tcggctttgc tcatgcctgc
     3061 ttctttcagt acacggtatc tttcggactg acgcatggct cgtgtcatta tatcgtcaat
     3121 ttcctgtgag gtcaggtcac gtgaaaatgg cgcgtagctc cgtccccgtt tttcactgaa
     3181 aaaacttttc tgtaatctct gcatgtgctc atctacggct tcttcggcgt atcgttgcat
     3241 tcgtgaatca atggttgtgt agatttttag tccgtcagta taaatattat agtttgaacc
     3301 gtcgggtttt ttgttttttt tacaaaatcc atacagcgga ttattttccc acgcccattt
     3361 atcgtccagg tatttttgtt tttgccagtc ggcgtaatca cttcgctgag gttcaccggc
     3421 cattaacatc tgtcgaagat attcacgata ataggtagcc agaccttctt tgtgatcgac
     3481 ccgcatgtaa ttgagcttca gaggtaactt gctcaacgag tcctgctgtt gacgggtgat
     3541 tatatcagct tttcgcatct gatcgaacac tacatttcgc cgtccgattg ttctttcttt
     3601 atgccgtacc ggattgaagt aggatggatt tttacacata cctatcagtg tggcagcttc
     3661 ttcaatttta agtttgtcgg cagtagtatt gaaataaaca tgtgctgccg attttatacc
     3721 aactgcattg ttgagaaaat caaactgatt gagatacatg gcaataattt cctccttcga
     3781 atacattctt tccagattga cggcaattac ccattcaatg ggtttttgca aagcacgttt
     3841 gaatatattg ctggcactgg gcgaataaag cagttttgac aactgttgag taatagtact
     3901 gccaccaccg gtattggaac gggtgaataa tccgaaaaat acacgtatca gggctttacc
     3961 atcgatgccg gaatgcttgt aaaaacgtac atcctcggta gcaatcagtg cattaatcac
     4021 atctttgtcg atatcgttgt aattgatggg tacccggttt tctttcgcat acgagtatct
     4081 tccaagaagt accatgtctg atgagtaaat ttcagtggcc gatttgttga tcggattttg
     4141 taattctttt atatccggca aatatcccag ccaaccaata ctaatcaata taaacatcaa
     4201 taccaatgcc ccgataccgg taaaaaaagt acgccagaag tattttttga ttttttttcg
     4261 tgtctcgtct gtaagtgcca tatattaatc tataagtcta aaattttatc agaacacaaa
     4321 aatacgatta ttttttgctt aaatatttaa cagattgcgt ttttaatgag atttaataat
     4381 gcacgctgct aatctactct tcgcaaagca tcaaatcagc aataatttga tctgaaatta
     4441 atattccatc aggtgttaaa cgcaaaaaac cattgttctg gtataataat ttttcattca
     4501 aatatttttt tgccgaaatc aggcaatatt ctgctttttc attgccgaaa attgtttcaa
     4561 cttgctttaa ttcaatgcct tcgcttgttc gtaacgatac cattatcaag tcattatact
     4621 tatcctgttc ggtgagtatt tccgtttcgt accactgaat gccggaattt atcttatcgc
     4681 aatactgccg tatggaagcc acattccact ggcgtgaaat cagattgtag gaatgtgctg
     4741 ccggtccaat gccaaggtac ggctgctgat gccagtaagc cgaattgtgc ctggagcgac
     4801 gatttggcag ggcaaaattc gatatttcgt agtgctcgaa gccattttcc acacacactt
     4861 ttactaaata ctgatacatg gcgttcatcg tttcatcatc aacttgcttt atttctcctc
     4921 tctgaagctg atgccagagc aaagtccctt tttcatacgt aagcccataa gcagaaatgt
     4981 gctgtacacc gagtgtgagc gctttgtcaa tctggtattt ccacgattgc aacgattggc
     5041 cgggcaatcc gtaaatcaaa tcaatgctga tattgtcaaa accacacgtt tgagcatctt
     5101 caatggcacg aacagcagtt tcggcagtat gacgccgccg aatggcagta agctgctcat
     5161 tgtcgaaact ctgaatgccg atgctcagcc ggttgatgcc gttttttcgc aaatgctgca
     5221 aatagctgat attcaaatca tcgggatttg cttcgagggt aatttccaca ttttcagcca
     5281 gttgaaagtg ggtgtttatt gaatttataa tgtccagaat attttccgca gatagggtag
     5341 aaggggtgcc tcctccgaag taaatggtcg aaacaagttc ttttttcaga tattcttttt
     5401 gtaaaaaaag ttcttttttc attgctgcca gcagctcaat ttgctggttg taatcagtag
     5461 ctgaaaaaaa atcgcagtaa ctgcatttac ttttacaaaa aggaatatgg atataaatac
     5521 cggccatgtc cgtcagggaa tgagaatttt gaaaacagac aggttgcctt tctctgtaaa
     5581 tgcttttaga atataaatac ctttcgccca atcgggtgtt gcaatggttt gattgtccgt
     5641 tattattgca ttatcataca tttttttgcc cgaaatatta taaatttcca ccttttctgt
     5701 actaccccgg acaattattt gcttgtttag aatagaataa taggtactaa tatcagcaac
     5761 ggcatttggt aaatttaccg aagacactgt attaaactca aaagctccaa gatcaggcgc
     5821 attgccaaaa tagttgtatc ccagatttat ccccttgtca atcaatgcac tggtactgac
     5881 aagatgcagc aagcttattt cgggaagtga gccgtcggct tgtcggtcgg tcagaagttg
     5941 cgtgtaatcc agacttgcaa aatctgccgc agtacaggaa aaacccgaat tccatgaatt
     6001 gtactcctgt gttaccactt tgcaggcaaa tttattggag gaagtcgagc ttagtgaagc
     6061 gcagtttttt atcaccagtg ttccaaaact gctgttcgaa aatccataat tcgtatcgtt
     6121 gagataaccg gtacagttat aaacagtcat cgtaccgttg ttgttgttct ggtcgaatcc
     6181 ttttacctta ttttttaccg aaacacaatg atgcaaagtg gcattatgag ccgtataatt
     6241 accaccaagt ttaaatccgt taccatttcc ataatttgtg ataatgtatc ggcccgatgt
     6301 gtttttaaat tgataaaacc aggtggcgct gtcggttttg aaacgaatgt gatccgtcat
     6361 atcgtaagat gccggaccgt tggcataaca ccagcagttg tcacaggttg tatttccggt
     6421 tttttcataa tgatcccagc catcgtcact attcagccac gaccgacagc cgatgtatga
     6481 attcgttccg gcatttgtgt attgcttatc ggcaaatcca tctgcattgc caccatagtc
     6541 agggatggct gttccaccgg ttttataatc aaaattttcg tacgaatcgc agtttataat
     6601 caggtttgag ctgccagttt tgagttgcaa accggaatca cagttccatc tgaagatgca
     6661 tttttctatc cggttattgc ttccggtcac tatcataccg ttatcgccgg ctccctgaat
     6721 agttatacct ttcacaaaca aataacttcc cgaaagtttt acaccggcat tattgctatt
     6781 gtaaggttga tttctgaaat caagtaccgg agtttcgttg ggataagccc aaatattaat
     6841 aaaagctgtt gagtttccgc tttttgtgat tgataacgag gtggatagca tatacatacc
     6901 accccggata ta
//
LOCUS       DAOPZK010000100         6892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_13423_length_6892_cov_14.175223, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000100 DAOPZK010000000
VERSION     DAOPZK010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13423_length_6892_cov_14.175223"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1361
                     /locus_tag="PKH68_06785"
     CDS             99..1361
                     /locus_tag="PKH68_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX12680.1"
                     /translation="MKRLLISIVILLFVFSVQSQEITLNECQQLARTNFPLIRQQALV
                     NEAANTSAAALLSRYFPQVQVAGQATWQSDVTKIPITIPNLTIPSLEKDQYKVYAEAS
                     QVIFDGGEIGLQRKTILANANVEQQKIEVEMYNLQDRINQTYFGILLMDEQLRQNELI
                     QNDLNAALEKIKVMVKNGVSLVTNQQNLEAELLKVNQYKTEINTQRQSLIKVLSLLIG
                     KELTNQTSFIRPLSITSSGDNQRPELKLLDIQINNLNLQNKQLTAQNLPKINLFAQGG
                     VGKPALNMFSNNFEPYLIAGAKINWQFSRLYTLGKERSNIEIQRKSLNLQKELFNLNN
                     SISQTRQSAEIQKLKQMLQTDDEIIDLRNKIKKTSATQLENGVITSADFIREANAENQ
                     ARQSKALHEMQLLLAEYELKWIGQNPPP"
     gene            1451..2386
                     /locus_tag="PKH68_06790"
     CDS             1451..2386
                     /locus_tag="PKH68_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNX12681.1"
                     /translation="MKLSINRRNQTPPLGGWGAVYFLFLLLSASCKSNDTEAFASGYF
                     EAVETIVSAQANGSILELNIQEGQELTAGEKVGQIDSTQINLTIKQIQSQKGAVLSRK
                     PDISSQTAILKEQLIQANRELQRVENLFAGQAATQKQLDDARSAVAVLNRQLNAQQTT
                     LDKTTASVGKETNPLEVQISLLEDQLRKCRIINPVSGTVLVKYVEPSEMAVMGKPLYK
                     IADLSTLILRAYITAGQLGELKLGQKVKVLTDKGKDKPSEYEGTITWISSKAEFTPKT
                     IQTRDERSNEVYAVKIAVKNDGYLKIGMYGEIMAP"
     assembly_gap    2388..2487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2719..3675
                     /locus_tag="PKH68_06795"
     CDS             2719..3675
                     /locus_tag="PKH68_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX12682.1"
                     /translation="MAGLGGQEHYISVSSLSKSYDKGAVLAVKNVSFSVNKGEVFGLI
                     GPDGAGKTSIFRMLTTLLLPDSGNAQVLGYDVVKDFRQIRNEVGYMPGRFSLYPDLSV
                     EENLKFYATIFGTTIEENFHLIRDIYVQIEPFKKRKSGKLSGGMKQKLALCCALIHKP
                     KVLFLDEPTTGIDPVSRKELWEMLLKLKEENISILVSTPYMDEAVRCDRIALIQQGEI
                     MKTDTPQAIVDQYDKPLYAVGADDTYHLLKALEQYPERINSYAFGEFARVVTTGTETH
                     LYEFLNNKGLKNIKITTVKATVEDCFIDLSIGHKSVITAPER"
     gene            3781..4518
                     /locus_tag="PKH68_06800"
     CDS             3781..4518
                     /locus_tag="PKH68_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX12683.1"
                     /translation="MYTITADKLTKKFGNFTAVDAISFEVKEGEIFGFLGANGAGKTT
                     AMRMLCGLSYPTGGKAIVAGFDVYRQQEDIKKNIGYMSQKFSMYENLTVRENIEFYGG
                     IYGLRMKEIKPRMKSMLQSLGLESEAGKLVSSLPLGWKQKLAFSTAIFHNPKLVFLDE
                     PTSGVDPVTRRQFWEMIYEAASRGITVFVTTHYMDEAEYCNRISLMVDGRIDALDAPG
                     NLKKQYQAATMNEVFYALARGAKRIAP"
     gene            4545..5705
                     /locus_tag="PKH68_06805"
     CDS             4545..5705
                     /locus_tag="PKH68_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX12684.1"
                     /translation="MNNEQSINQPHSPTPPSGGRGASLRAFLRKEFLHVFRDKRTLLV
                     MFGLPIAQILIFGFALTNEVKNARVLIVNPSTSIESSKLIEQIDASSYFTVTHIASSR
                     KDIEHYFRNNEAQCALILPEDFSSSTVSPPQIQIVTDATDPNFAKTVVFYLSAIITAF
                     QQQSIPAGSIPMVIKTEIRMLYNPSLMGAMNFVPGVIALIMMIVCTTLTAIAIVREKE
                     MGTMETLLVSPVKPLALLIAKTIPYAVLSLVNLSFILLLSNLVLDVPVRGSVVLLLFE
                     CFLYIISCLALGLMISNIATSQAVAMMISMAGILLPTLLLTGFLFPLENMPWIFQWLS
                     NIVPARWFFIIVKSVMLKGMGLQDIWMETSILIGMTVILLAISLKKFKIRLE"
     gene            5702..6814
                     /locus_tag="PKH68_06810"
     CDS             5702..6814
                     /locus_tag="PKH68_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX12685.1"
                     /translation="MRVLLFILRKEFIQIFRDKMMLPMLLLMPVLQLLILPFAADLDV
                     RQLKITIVDNDHSPYSRSLISKISATDLFEIYAISGSYDEALKNVEKDETDIILQIPQ
                     GFERNLIREKTESVAITADAINGVKAGLGTSYLNAIIQNFNREIVMEWANTPEVSASP
                     DIRYSFWYNPALRYKNFIVPAILVLLVTVISGFVSALNIVSEKEKGTIEQINVTPLKK
                     WQFIIGKLLPFWIIGLFDFSLGLLIVYVVYAIPVVGSLSTLYLFAVVYITGILGFGLL
                     ISTVSNTQQQSMFVAFFFVMIFILMSGIFTNVESMPPWAHFISNLIPATHFMHAVRAI
                     ILKGSVFLDLWVDMVYILCFDVVMITAAVINYHKTA"
BASE COUNT         2030 a         1445 c         1491 g         1826 t
ORIGIN      
        1 cggaaaaatt acatatgcgt tgataatttc acaaaaaaaa atcaaatgaa ttaacgtaga
       61 acatttcaga aatttcagga ccaactaatt tctcaaaaat gaaacggtta ctaatttcca
      121 tcgtaatact cctgtttgtt ttttctgttc aatcacagga aatcacttta aatgaatgtc
      181 agcaactggc acgaaccaat ttcccgctta tacgccagca ggctttggtg aacgaagccg
      241 caaatacttc cgcagcggcg ttactaagcc gttattttcc tcaggttcag gttgccggac
      301 aggccacgtg gcagtcggat gtgaccaaaa tccccattac gatcccaaac ctgaccatcc
      361 cttcacttga aaaagatcag tataaagttt atgccgaagc atcacaagtc attttcgacg
      421 gtggcgaaat tggcctgcaa cgaaaaacta tccttgctaa tgccaatgtg gagcaacaaa
      481 aaatagaggt ggaaatgtat aatctacagg accggattaa ccagacatac tttggaatac
      541 tgctgatgga cgaacaactc cggcaaaatg aactgataca gaatgatttg aatgctgctt
      601 tggagaagat taaggtgatg gtgaaaaacg gggtctcact ggtcaccaat cagcaaaacc
      661 tggaagctga actgttgaaa gtaaatcaat ataaaacaga aataaatacc caacgacaat
      721 cgctgataaa ggtactgagc ctactcatcg gtaaggagct aaccaatcag acaagtttca
      781 tccgacctct ctcaataact tcaagcggag ataatcagcg tccggaactt aaactgctcg
      841 atattcagat aaacaatctt aacttgcaga acaaacagct taccgcccaa aatcttccaa
      901 aaattaatct ttttgctcag ggaggagtag ggaagccggc cctgaacatg ttcagcaata
      961 actttgaacc ttacttaatt gcaggtgcca aaatcaactg gcagttttcg aggctttata
     1021 cccttggtaa agaacgatca aatattgaaa tacaacgaaa atcgctgaat ttacaaaagg
     1081 aactatttaa cctcaataac tccatcagcc aaacccgtca atcggctgaa atacagaagt
     1141 tgaaacaaat gttgcagacg gatgatgaga ttattgacct ccgaaataaa attaaaaaaa
     1201 ccagcgccac acagctcgaa aacggcgtga ttacctccgc cgatttcatt cgtgaagcca
     1261 acgccgaaaa tcaggcccgt cagtcaaaag cactgcacga aatgcagttg ttgctggcag
     1321 aatatgaatt gaagtggata ggacaaaatc caccccccta aactccctcc cgtattacgg
     1381 gataaactcc gcgggactat aagaatttgt tttgaaaata ttcttacaac ttagttcaaa
     1441 tatagaaaaa atgaaattat caattaatcg caggaaccaa actccccctt tagggggttg
     1501 gggggccgtt tattttcttt ttctactact tagtgcatcc tgcaaatcga atgatacaga
     1561 agcctttgct tccggatatt tcgaagcggt ggaaacgatc gtttcggcgc aggccaatgg
     1621 aagtattctg gaactgaata tccaggaagg tcaggaattg actgcaggcg aaaaagtggg
     1681 acaaattgac agtactcaaa ttaatctgac aattaagcag atacagtcgc aaaaaggggc
     1741 agtactgagc cggaaacccg acataagttc gcaaaccgcc attctgaaag agcaactcat
     1801 tcaggccaac cgcgaattgc agcgcgtaga aaaccttttt gccggacaag cggccactca
     1861 aaaacagctg gatgatgccc gatcggcagt tgcagtactc aatcgccagc tcaatgccca
     1921 acaaactacg ctcgacaaaa ccacagccag cgttggcaaa gaaaccaatc cgctcgaagt
     1981 acaaatatca ctgctggaag atcaactccg gaaatgccgc attatcaatc ctgtttcagg
     2041 tacggttttg gtgaaatatg tggaaccttc agaaatggcc gtgatgggca aaccgcttta
     2101 caaaatcgct gatttgtcga cccttatatt gcgggcgtac attacagccg gccagttggg
     2161 cgaactcaaa ctggggcaga aagtgaaagt gctgaccgac aaaggcaaag ataaaccttc
     2221 cgaatacgaa ggaaccatca cgtggataag cagcaaggca gaattcacac ccaaaactat
     2281 ccagacccgc gacgagcgct ccaacgaagt gtacgctgta aagatagccg tgaagaacga
     2341 tggatatctg aaaataggga tgtatgggga gataatggcc ccctaaannn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnntcc cccgaagggg gacttttaaa tacctgcaat
     2521 ttggatcttg tttattgaaa atatcaggat gttttgtatg gttgtaaata attcaacttt
     2581 caggtaagtt ttttttctct aaaattaatg atgattttta attcaaatac taatcagccg
     2641 cagtctctaa aagtccccct tgagggggat ttagggggct tcactccaaa agtccccctt
     2701 caggggactt gcctgccttt ggcaggttta gggggccagg agcattatat ctctgtttct
     2761 tccctctcca aatcctacga taaaggggct gttttggccg taaaaaatgt cagtttttcg
     2821 gtaaataagg gtgaagtatt cgggttgata ggtccagatg gagcaggaaa aacatccatt
     2881 ttcaggatgt tgacaacgct gctcctgccc gatagcggaa acgctcaggt tttgggatat
     2941 gatgtagtga aagatttccg ccagatacgc aacgaagtgg gttatatgcc tggccgtttt
     3001 tcactctatc cggacttgag tgtggaggaa aatctaaagt tttatgctac cattttcggt
     3061 acaactattg aagaaaattt ccatctgata cgggatattt atgtgcagat tgagccattt
     3121 aaaaaacgaa aatcgggcaa actttcgggt ggaatgaagc aaaaactggc actttgctgc
     3181 gcgctaattc acaagccaaa agtgcttttt ctcgatgaac cgactaccgg aattgatcct
     3241 gtatcccgaa aggaactttg ggaaatgctc ctgaaactga aagaggaaaa tattagtatt
     3301 ctggtttcca caccttatat ggacgaggct gtgcgatgcg atcggatagc gctcattcag
     3361 cagggagaaa taatgaaaac agatacccct caggctattg tcgatcagta tgataagcct
     3421 ctgtatgcag ttggagcgga cgatacatat cacttactca aagcgctgga acaatatcct
     3481 gagaggatca acagctatgc atttggcgaa tttgcccggg tagttacaac aggcaccgaa
     3541 acccatttgt acgaattttt gaataataag gggctgaaaa acatcaaaat tacaaccgta
     3601 aaagctactg tagaagattg ctttattgat ctgagcatcg gacataaatc agtgattact
     3661 gctccagaaa gatagtaaaa caaagaaaaa aattcagttt aaatttcatt aaataaagga
     3721 taaaaagaag acagttttta ttactaaaac atcggattat ttttcaaaaa aaacggataa
     3781 atgtacacca ttacagctga caaactgact aagaaattcg gaaattttac cgccgttgat
     3841 gccatatcat tcgaagtaaa agagggcgaa attttcggtt ttctgggcgc caacggagcc
     3901 ggaaaaacca ctgccatgcg tatgctttgt ggtttgtcgt atcctaccgg aggcaaggca
     3961 atcgtagccg gatttgatgt gtacagacag caggaagata ttaagaagaa catcggatat
     4021 atgagccaga agttttcgat gtatgaaaat ctgacggtac gcgaaaatat agaattttac
     4081 ggcggaattt acggcttgcg aatgaaggaa ataaaacccc gtatgaaatc catgctccaa
     4141 tcgcttgggc tggaaagcga agccggcaaa ctggtcagtt cgctgccttt gggctggaaa
     4201 cagaaactgg ccttttcgac cgccattttt cataatccga aacttgtttt tctggacgaa
     4261 ccgaccagcg gagtagatcc cgtcacgcgt cgacagttct gggaaatgat atatgaagct
     4321 gcttcacggg gaataaccgt atttgtaact acccattaca tggacgaagc tgagtattgc
     4381 aaccgcattt cgctgatggt ggatggacgg attgatgcac tggatgcacc cggaaatttg
     4441 aaaaaacagt atcaggcagc cacaatgaac gaagtgtttt acgccctggc acggggtgcg
     4501 aaaagaattg ccccctaacc cccctaaagg gggaaataag aaaaatgaac aacgaacaaa
     4561 gcatcaatca accacactca ccaactcccc cttcaggggg gcggggggcc tctctgcgtg
     4621 cttttcttcg caaggagttt ctgcatgttt ttcgggataa gcggacgttg ctggtgatgt
     4681 tcggacttcc cattgcgcaa attctgattt ttggatttgc gctgaccaac gaagtgaaga
     4741 atgcccgtgt gctaatagta aatccctcaa cgagcatcga aagcagcaaa ttgattgagc
     4801 aaattgatgc aagttcttat tttacagtca ctcatattgc atcctcgcgc aaagatatcg
     4861 aacattactt cagaaacaac gaagcccagt gtgcgttaat tcttcctgaa gatttttcat
     4921 catccaccgt ttcgccgcct caaatccaga tagtgaccga tgccaccgac cccaactttg
     4981 ctaaaacggt tgtgttttat ctttctgcta tcatcacggc atttcagcaa cagtccatac
     5041 cagccgggag catcccgatg gtgataaaaa ccgaaatccg gatgctttat aatccatcac
     5101 tgatgggcgc gatgaatttt gttcccggag taatagcact gataatgatg atagtttgta
     5161 ctacgctcac agctatagcc attgtgcgcg aaaaggagat gggaacgatg gaaaccctgc
     5221 ttgtttcgcc cgttaaaccg ttggctttgc tcattgccaa aaccattccc tatgcagtat
     5281 tgtcgctggt caatctttcg tttatcctgc tgctgagcaa tctggtgctc gatgttcccg
     5341 ttcggggaag tgttgtgctg ctacttttcg aatgtttttt atacattatt tcctgcctgg
     5401 cgctgggcct gatgatatcc aacatagcca cttcgcaggc tgttgccatg atgatttcga
     5461 tggcaggtat tttgttgccc accttgctgc ttacgggttt tctgtttcca ctggagaata
     5521 tgccctggat atttcagtgg ctctccaata ttgtaccggc gcggtggttt ttcatcatcg
     5581 ttaagtccgt tatgctcaaa ggaatgggac tgcaggatat ctggatggag acttcgatcc
     5641 tgatagggat gactgtgata ctgttggcca tcagtttgaa aaaatttaaa atccggctgg
     5701 aatgagagtg ctcctgttca tattgcgcaa ggaatttatc cagattttca gggataaaat
     5761 gatgctcccg atgcttctgc tcatgcccgt tttgcaactg cttatccttc cttttgctgc
     5821 cgatctggat gtgcgtcaac tcaaaatcac cattgtggac aatgatcaca gtccttattc
     5881 ccgaagcctt atttcgaaga tttcggctac agatttgttt gaaatttatg ctatttcggg
     5941 ttcctacgat gaagcgctga aaaatgtgga aaaggacgaa actgacataa tcctgcaaat
     6001 tcctcaaggt tttgaacgaa atcttatccg cgaaaaaacc gaatctgtag ccattaccgc
     6061 cgatgctatc aacggggtga aagccggctt gggcacaagc tacctcaatg caattattca
     6121 gaatttcaat agggagatag tgatggagtg ggctaataca ccggaggttt cagccagtcc
     6181 ggatattcgg tactcatttt ggtacaatcc tgctctgaga tataaaaatt ttattgtacc
     6241 ggcgattctg gtgcttttag tgaccgttat cagcggtttt gtgtcggctt tgaacattgt
     6301 gagtgaaaaa gagaaaggga ccatcgaaca gataaatgtc actccgctga aaaaatggca
     6361 gtttatcatc ggaaaattac ttccattttg gattatcggt ctttttgatt tcagcctggg
     6421 cttgctcatt gtatatgtcg tatatgccat accggttgtg ggaagtctga gcaccctcta
     6481 tttgtttgca gtagtgtata ttaccggaat attgggattt ggactgctta tatccacggt
     6541 gagcaatacc cagcagcaat ccatgttcgt cgccttcttt tttgtaatga tatttattct
     6601 tatgagtggc atttttacca atgtagaaag catgcctccc tgggctcatt tcatttcgaa
     6661 tttaattccg gccacccact ttatgcatgc tgtgcgggct attattctta aaggaagcgt
     6721 ttttctggat ctttgggtgg atatggttta tattctttgt tttgatgtgg tgatgattac
     6781 tgctgcagta atcaattatc acaaaacggc ttaaaaagaa tattggaagt ttctttcaat
     6841 aaaaatgcag gttacagttt tccgtaacct gcatttttat tctcactaat tc
//
LOCUS       DAOPZK010000101         6888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_13434_length_6888_cov_25.916142, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000101 DAOPZK010000000
VERSION     DAOPZK010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13434_length_6888_cov_25.916142"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..2417
                     /gene="topA"
                     /locus_tag="PKH68_06815"
     CDS             66..2417
                     /gene="topA"
                     /locus_tag="PKH68_06815"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNX12686.1"
                     /translation="MSNNLVIVESPAKAKTIEKFLGKDFEVTSSMGHIRDLGEKGLGI
                     DFENNYAPQYEVSADKKKLVTELKKKVKTADMVWLASDEDREGEAIAWHLSEELKLKK
                     EKTKRIVFHEITKDAILNAIEHPRDIDLNLVDAQQARRVLDRIVGFELSPVLWRKVRP
                     SLSAGRVQSVAVRLIVEREREINQFAAVAAYKVTAVFSSVDASGAPVELKAELPQRFD
                     KKADALAFLEACKNAQFSIDSIEKKPAKKSPAPPFTTSTLQQEASRKLGFSVSQTMMV
                     AQKLYESGKITYMRTDSVNLSALALGTAKSEIISMAGEKYVHSRNFTTKSKGAQEAHE
                     AIRPTYISAHSVEGTAQEKRLYDLIWKRTIASQMADAELEKTNIYINVSGSKYQFIAS
                     GEVIVFDGFLKVYLESTDDEQDADTSGILPTMKKGEKLTANEILAQERFTQRPARYTE
                     ASLVRKLEELGIGRPSTYAPTISTIQNRKYVEKGDRPAVKRDFNELKLKDGKITDKLK
                     SENTGAEKSKLFPTDIGLVVNDFLVEYFPDIMNYNFTANVEIEFDHIAEGKVKWTKSI
                     DNFYKKFHPVVENTLKNSERQVGERILGTDPKSGRQISVKIGKYGPIAQIGTSEEEEK
                     PVFASLRPNQSIESVTLEETLELFKLPRELGEFEEKGMTVAVGRFGPYIRHNSAFYSL
                     PKTDDPLEVTAERAIEIIEAKREADKNKIISTFGENGEIQVLNGRFGPYIAFEKNNYK
                     IPKKTDPLTLTEDQCRELIASQADTVGKKKKPPYKGRSKKTKA"
     gene            2458..3489
                     /locus_tag="PKH68_06820"
     CDS             2458..3489
                     /locus_tag="PKH68_06820"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HNX12687.1"
                     /translation="MKHILLFIALIAPFFATAQQKQQLTDGESVLIYSLPKTVLNIHI
                     SSERITETPGEFYLYSERYLATTNVITAPKTYYRIKGITITPQTKPDSKRTFKITPVK
                     KSLTTRITVNDKGILCGINVPPVDVPEIVPPFNLEKANQTSIKLLPLEEEYMMAGSTV
                     KMAEGVAKQIYSIREGRLDLLAGQADTMPKDGESMKLMLEQMDRREQELTQLFTGKTT
                     VEALTKTITYAPDSASNAEVVFRFSAFNGLSSKEDLSGTPYYITVKYNPIETATAKKK
                     KEKGEQVFSVLPVKAKITVEEGSNVIYNEVMDIPQLGILVPIPLETMDKKTGKVLVSP
                     ETGRLLSIQ"
     gene            complement(3546..4433)
                     /locus_tag="PKH68_06825"
     CDS             complement(3546..4433)
                     /locus_tag="PKH68_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNX12688.1"
                     /translation="MKKRIAFIINPMSGTRTKAGLPGILRRELDPKQYDDFRILHTEY
                     KGHGTQLSAQLVREGYNVVVAVGGDGTVNEVARSLIHTNVALGIIPAGSGNGLSRHLN
                     IPTNVVKATQLLNKSEVIKTDYGIVNGKPFFCTCGTGFDAYVSMEFARGKKRGIIGYL
                     EKMVTGYFNYEPQNYKLSGDDINLDGKAFVITFANAAQWGNNAYIAPEASVQDGLLDV
                     SIISKVPVIAIPTLALQLFTKNIHNDLLVTTLRTSEITLIRDHPGPFHYDGEPYEEGN
                     KIEIRMVPDGLNVLVKKRF"
     gene            4558..6696
                     /gene="rnr"
                     /locus_tag="PKH68_06830"
     CDS             4558..6696
                     /gene="rnr"
                     /locus_tag="PKH68_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNX12689.1"
                     /translation="MSKRNKNSGSTRERKSDLIRNISNIFSENPEKTYNYKQISTLLN
                     ITSESQRAFVNQLLYELADEDYLKEISRGKFRINSRSGYITGTIDRHGVKTYLIPEDG
                     GEMIFISEQKTNTAMRNDVVKARLYAQRKGQEPEGEVVEIIKRAQDTFVGELDVSDNY
                     AFLIISNRIINNDIFIPKDKLKGGKSGQIAVVKLQEWKPNLKSPVGEVIDILGDKGNN
                     DTEMHAILAEFGLPYVYPANVEAEAAKIDAGITPEEVAKRIDLRGVTTITIDPRDAKD
                     FDDAISLRKLSNGLWEAGVHIADVTHYVRPGSIIDTEGEERATSVYLVDRTVPMLPEH
                     LSNGICSLRPNEDKLTFSVLFQMNDKAEVLNYKIARTVTRSVRRFTYEEAQSIIETGE
                     GEYKEEVLMLDKLAKQLREERFKTGAIAFDRVEVRFEIDGKGKPLSVYFKEQKDSNKL
                     VEEFMLLANKTVATHIGKPGKGLKAKTFVYRIHDVPDPDKLQNFSAFIRKFGYNLKAT
                     GKNTQISASINQLLDDVQGKKEQNLIETLAVRSMAKAIYSTQNIGHYGLAFDYYTHFT
                     SPIRRYPDMMVHRLLQRYLDGGSGVDAREYEDKCEHSSNREQLAANAERASIKYKQVE
                     FMSDRIGKVFDGVISGITEWGIYVELIENKCEGMIPIRDLDDDFYTMDEKNYCLIGRR
                     FHKKYQLGDEVTVKVARANLDKRQLDFVLA"
BASE COUNT         2133 a         1548 c         1564 g         1643 t
ORIGIN      
        1 gtacctgttt gtttttgtta ttttgcaccc gatttcgaat tcgtaaataa aaattaagcg
       61 taattatgtc caataattta gtgattgtag agtcgcctgc caaggcaaag actattgaaa
      121 agtttctcgg aaaagatttt gaagttacgt ccagcatggg acatattcgc gatcttggag
      181 agaaaggact gggaattgat tttgagaaca attacgctcc acagtatgaa gtttcggcgg
      241 ataaaaagaa actggtgacc gaactgaaaa aaaaggtaaa aacagccgat atggtttggc
      301 tggcttccga tgaggaccgc gaaggagaag ccattgcgtg gcatttatcc gaagagttga
      361 aactgaaaaa agagaaaacc aaacgtattg tctttcacga aattacaaag gatgccatac
      421 tgaatgccat tgagcatccg cgcgatatcg acttgaattt ggtggatgct cagcaggcac
      481 gccgcgtact cgaccgcata gtgggttttg aactctcacc ggtgctttgg cgcaaagttc
      541 gcccgagctt gtcggcagga cgggtgcaat cggtagcggt tcggctcatt gtagaacgcg
      601 aacgtgaaat caatcagttt gccgccgtag cagcctacaa agttacagcc gttttttcgt
      661 cggtagatgc gagtggtgct ccggtagaac tgaaagccga attaccacaa cggtttgata
      721 aaaaagccga tgcgcttgct tttctggaag catgcaaaaa tgcacaattc agtattgaca
      781 gcatcgaaaa gaaaccggca aaaaaatcac cggcaccacc ttttaccacc tccacgttgc
      841 agcaggaagc ttcccgtaaa cttggcttct cggtttcgca gaccatgatg gtggctcaaa
      901 agctgtatga gtcgggaaaa atcacttata tgcgtaccga ctcggtcaat ctttcggcac
      961 tggcactggg aacagccaag agcgaaatca tcagcatggc cggtgaaaaa tatgtacatt
     1021 cacgcaattt taccaccaaa tcgaaaggtg cccaagaagc tcacgaagcc attcgcccaa
     1081 cctatatcag cgcacacagc gtagaaggta ctgcacagga aaaacgcctg tacgacctga
     1141 tctggaaacg caccatagcc tctcagatgg ccgatgccga actggaaaaa acaaacatat
     1201 atattaatgt atccggcagt aaataccagt ttattgcatc gggcgaagta attgtttttg
     1261 acggttttct caaagtttat ctggaatcga cagatgatga gcaggatgct gacacaagcg
     1321 gcatattgcc cactatgaaa aagggagaaa aactcactgc caatgaaata ctggctcagg
     1381 aacgcttcac ccaacgtccg gcacgttaca ccgaagcaag ccttgtgcgc aaactcgaag
     1441 aactgggtat cggtcgtccg tcaacctacg caccaaccat ttccaccatt caaaaccgga
     1501 aatacgtaga gaaaggagat cgtccggctg tgaaaagaga tttcaatgaa ctgaaactga
     1561 aagacggaaa aattaccgat aaactgaaat cggaaaatac cggagccgag aaatcaaagc
     1621 ttttcccaac cgatattggt ttggtggtca acgacttttt ggttgaatac tttccggaca
     1681 ttatgaatta taatttcacg gctaatgtgg aaattgaatt tgaccacata gccgaaggaa
     1741 aagttaaatg gactaaatcc atcgacaatt tctataaaaa attccatcct gtagttgaaa
     1801 ataccctcaa aaattcggaa cgccaggtag gcgaacgcat tcttggaaca gatccaaaat
     1861 ccggtcgtca gatttcggta aaaattggta aatatggtcc gatagctcaa atcggtactt
     1921 cggaagaaga agaaaaacct gtttttgcat cgcttcgtcc caatcagtcc atcgaaagtg
     1981 tgacgctgga agaaacactg gaattgttca aattgccccg tgaactggga gaatttgagg
     2041 aaaaaggaat gacagtggct gtgggacgtt tcggccctta cattcgccac aattctgctt
     2101 tttattccct tcccaaaacc gatgatccgc tcgaagtgac tgcagaaaga gccattgaaa
     2161 tcattgaagc aaaacgcgaa gccgataaga acaaaataat cagcacattc ggagagaatg
     2221 gagaaattca ggtattgaac ggccggttcg gaccatacat tgcttttgaa aaaaacaatt
     2281 ataaaattcc gaagaaaacc gatccactga ctctaaccga agatcaatgc cgtgaattga
     2341 tagcctcgca ggccgatacc gtggggaaaa agaaaaaacc accttacaaa ggcagaagca
     2401 aaaagacaaa agcctgatca gaattatccg tttactaata aaaaaggaaa ccatatcatg
     2461 aaacatatac ttttattcat tgctcttata gctccttttt ttgccacagc tcaacaaaaa
     2521 cagcaattga ccgatggaga atctgttctg atctattctt tgccgaaaac ggtactgaac
     2581 atccatatca gttccgaacg cattaccgaa acccccgggg aattttatct ctattccgag
     2641 cggtatctgg ctaccaccaa tgtgataact gcccctaaaa cctattaccg catcaaagga
     2701 ataacgatta caccgcaaac caaacctgat tcaaaacgta cattcaaaat tactcctgta
     2761 aaaaaatcac ttactacacg tatcacggtg aatgataaag ggatactatg cggcatcaac
     2821 gtgcctccgg ttgatgttcc ggaaatagtt ccacctttca accttgaaaa agccaaccag
     2881 acaagtataa agctattgcc gctggaagaa gagtacatga tggccggctc gacggtaaaa
     2941 atggccgaag gggttgccaa acagatttac agcattcgtg aaggacgttt ggatttattg
     3001 gccggtcagg cagatacaat gcctaaggac ggggagtcca tgaaacttat gctggaacaa
     3061 atggataggc gggagcagga actcacccaa cttttcaccg ggaaaactac cgttgaagct
     3121 ttgactaaaa ccatcaccta tgcgcccgat tcagcatcaa acgccgaagt tgtgttccgg
     3181 ttttcagcat tcaacggact tagttcaaaa gaggatctga gcggaacacc gtattacatt
     3241 acggtgaaat acaatcccat agaaactgcc actgccaaaa aaaagaaaga aaaaggcgaa
     3301 caggtatttt ctgttttgcc ggtcaaagcc aaaataactg ttgaggaagg ttccaacgtg
     3361 atttacaatg aagtgatgga catcccgcag ttgggtatac tggtgccaat accgctcgaa
     3421 acgatggaca aaaagaccgg gaaagttctt gttagtcccg aaacaggaag actgctatcc
     3481 atacaataaa tgaattcagg ggaataaaaa aaagagacaa tcggttggtt gtctcttttt
     3541 tttggttaaa atcttttttt caccagtaca ttcagcccgt cgggcaccat tctgatttca
     3601 attttattcc cttcttcata cggttcaccg tcgtaatgga atggaccggg gtgatcgcgt
     3661 atcagcgtta tttcactggt tcgcagggtg gtaacgagca aatcattgtg tatatttttg
     3721 gtgaaaagct gcagtgccag tgtgggaata gctatcaccg gcactttact gatgatggat
     3781 acatcgagca gcccatcctg cacactcgct tcaggtgcaa tgtaggcatt gtttccccat
     3841 tgtgctgcgt tggcaaaagt gattacaaat gctttgccgt cgagattaat atcatcaccg
     3901 ctgagtttat aattttgggg ttcatagttg aaatagccgg tcaccatttt ttccaggtat
     3961 ccgataatgc cccgtttctt gccgcgggca aactccatgc tcacataggc atcaaaacct
     4021 gttccgcaag tgcaaaagaa aggttttccg tttacaatgc cgtagtcggt ttttattact
     4081 tccgatttgt tgagcagttg cgttgctttc accacattgg tgggtatatt gaggtgtcgc
     4141 gacagtccgt tgcccgatcc ggcaggaata attcccagtg ccacattggt atgtatgagc
     4201 gaccgggcta cctcgttgac cgtaccgtcg ccgccaacag ccaccaccac attatagcct
     4261 tcccttacca actgtgccga cagttgtgtg ccgtgtccct tgtattcggt atgaagaatg
     4321 cggaagtcat cgtattgctt tgggtccagt tcccgtcgca ggatgcccgg caatccggct
     4381 ttagtcctgg tacctgacat cggattaatg ataaaagcaa ttcgtttttt cataggggaa
     4441 tgaatgaatt atagaatgcg aaaatacatt taattttccg gaaaataaaa ccttttttat
     4501 tccgatagaa acttaaggtc gccggcattg ttgtatattt gtaaaaaata aatcattatg
     4561 tcaaaaagaa acaagaactc gggaagtacc cgggagcgta aatcagacct tatcaggaac
     4621 atttccaata ttttttccga aaatccggaa aaaacctata actacaagca aatttccacc
     4681 ttgctgaata tcacctccga atcgcagcgg gcttttgtga accagcttct ttatgaactg
     4741 gctgatgaag attatctgaa ggaaatctca cgcggaaaat tccggattaa ttcccgaagc
     4801 ggatatatca ccggtacaat agaccggcac ggagtgaaga cttacctcat tcccgaagat
     4861 ggtggcgaaa tgattttcat ttcggaacag aagaccaata ccgccatgcg caacgatgtg
     4921 gtgaaagccc gcctctacgc acagcgcaaa ggtcaggaac ccgaaggtga agtggtggaa
     4981 ataataaaac gtgctcagga tacctttgtg ggcgaactgg atgtttcgga taattacgcc
     5041 tttctgataa tcagtaaccg cataatcaac aacgatattt ttatcccgaa agacaaactg
     5101 aaaggaggaa aatcaggaca gatagcggtg gtaaaactgc aggaatggaa accaaatctg
     5161 aaaagtccgg taggtgaagt cattgatata ctcggcgaca agggcaacaa cgacaccgaa
     5221 atgcatgcca ttctggccga attcggattg ccatacgtgt atcccgccaa tgtggaagct
     5281 gaagctgcca aaatagatgc cggtatcaca cccgaagagg tagccaaacg tattgatctg
     5341 cgcggtgtaa caaccatcac catcgacccg cgcgatgcca aggattttga cgatgccatt
     5401 tcactccgca aactgagcaa cggactgtgg gaagccggag ttcacattgc cgatgttact
     5461 cattacgtcc gccccggatc gataattgac accgaaggcg aagaacgtgc cacatcggtc
     5521 tatctggttg accgaacggt accgatgctg cctgaacatc tttcgaacgg catctgttcc
     5581 ctccgaccca atgaggacaa actcactttc tcagtacttt ttcagatgaa cgataaggct
     5641 gaagtgctca actataagat agcccgcacc gtaacccgca gcgtccgccg tttcacttac
     5701 gaagaagcac aaagcatcat agaaacaggc gaaggggaat ataaagagga agtactgatg
     5761 cttgacaaac tggcaaagca gttgcgtgag gagcgtttca aaaccggcgc cattgccttc
     5821 gaccgggtag aagtgcgttt tgaaattgac gggaaaggta aaccgctgag tgtatatttc
     5881 aaggaacaaa aagactcgaa caagctggtg gaagaattca tgctgctggc caataaaaca
     5941 gtggctaccc acatcggtaa accagggaag ggcctaaagg cgaaaacctt cgtttaccgc
     6001 atacacgatg tacccgatcc cgacaaactg caaaacttct cggcatttat ccgcaagttc
     6061 ggctacaacc tgaaagcaac cggtaaaaac acccagattt cggcctccat caatcaactg
     6121 ctggatgatg tacagggcaa gaaagaacaa aacctgatag aaacgctggc agtgcgctct
     6181 atggcaaaag ctatttattc cacacagaat attggtcatt acggactggc atttgattat
     6241 tacacccatt tcacctcacc catccgccgt tatcccgata tgatggttca ccgcctgctg
     6301 caacgctatc tggatggtgg cagcggtgtg gatgcccgcg aatacgagga caaatgcgaa
     6361 cacagttcga accgcgaaca gctggctgcc aacgccgagc gtgcttccat caaatacaaa
     6421 caggtggaat ttatgtccga caggattggc aaggtattcg atggtgtaat ttcgggtatc
     6481 accgaatggg gaatatacgt ggaacttatc gaaaataagt gtgaagggat gataccgata
     6541 cgcgatctgg acgacgattt ctataccatg gacgaaaaaa actattgcct gatcggacgc
     6601 cggttccata aaaaatacca gctgggcgat gaagtgaccg tgaaagtagc cagggcaaat
     6661 ctggataaaa gacagttgga ttttgtgctg gcgtaaaaag aaggaagcac cctaaatccc
     6721 ctccctaaac aagttcggga caggcacagg ggggacttcc cttattcgct aacaggttcg
     6781 caaaaggatt ttagaaaaat atttcgcatt aattatttga gaaatcaaaa taatgcctta
     6841 tctttgcagt ccgaaacaac ggaatggccc cgtggctcaa ctgaatag
//
LOCUS       DAOPZK010000102         6866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_13477_length_6866_cov_21.606372, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000102 DAOPZK010000000
VERSION     DAOPZK010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13477_length_6866_cov_21.606372"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..924)
                     /locus_tag="PKH68_06835"
     CDS             complement(163..924)
                     /locus_tag="PKH68_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNX12690.1"
                     /translation="MLSKSKIKWIHSLELKKNRDEQNLFVAEGIKLVSDLLPHLQCRF
                     LAFCSPDFVFNLYSNTVAEAERVTEEEYKKITLQKTPQGVLAVFEKPADTWNEAEIHN
                     SLSLALDDVQDPGNLGTIIRLADWFGIRHVFCSLHCADAYSPKTIQATMGAIAGVNVH
                     YVDLPSFLRQMKGKIPVYGTFMDGTNIYEKTLENNAILVMGNEGNGISAAVEALVTEK
                     LLILRFSPDTSASESLNVSTATAITLSEFRRRIKP"
     gene            1056..3320
                     /locus_tag="PKH68_06840"
     CDS             1056..3320
                     /locus_tag="PKH68_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNX12691.1"
                     /translation="MRLSKGSFVLALGLLALACNPIKYVPDNEHLLNKVKLESDNKTI
                     LKEDIATYVRQTPNNEIFGIFKMQLGIYNLSGPDTSKWINRRLKKMGEEPVIYNPSLT
                     TITEREISKLFSNKGYMDASVKSEVTFPKEKVADVTYTIQSNKPYTLNSYSVNISQPE
                     LAEIASDTAESLIKTGKLFDSDLFDQERDRIASKFRELGYYNFTKDLLQYYADSTQIS
                     HRIDLVLELNEQLKKADEDIRERVFRQYTINKVHFYCESDKTTDMQNTGITLDSLTLG
                     DYNLVYENKRNIRPTVLIEHTLIIPGRRYSDRAVERTYSSLNSLSAVKYVNVSYREVD
                     KDKLDCYVVITPNKLQSISADGEVTYSAGYWGFGGNINYGHRNAFKGSETVSVKGRMA
                     YEYQGTGQKAIEVGADVGLKFPTFLLPFSSLDMRRNIRANTEVTATMSYRDRPKEYTG
                     IVTGAGFKYSWSEKFQIKHNFDVLDISYVNYPKITGEYQDYINSNPTFAYNFEPHLIM
                     RTGYSGSYSGFTPTKPFRNYTTMNYGVETAGNILQGANSLLGAKPDSTGAFKVFGIRY
                     AQYVKTDFHISYHHIYDANNRLVYHAGLGIAIPYGNAERMPFEKRYFSGGANSVRGWT
                     AYQLGPGTYRSPGNYVDYNTQMGDIKLDLNMEYRTKLFWKLDGAFFLDAGNIWTIRNY
                     VAQPGGVFRFDKFLGQLGIAYGTGIRADFSFFVLRVDLGLKLFNPALERTEQWRTKFN
                     ANDFAINLAIGYPF"
     gene            3357..3809
                     /gene="dtd"
                     /locus_tag="PKH68_06845"
     CDS             3357..3809
                     /gene="dtd"
                     /locus_tag="PKH68_06845"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNX12692.1"
                     /translation="MRILIQRVKEASVTIGGRLKSQIGGGLLVFVGIEDADEEDDSCR
                     LASKLVKMRIFNDENGVMNLPVTEIDGEIMIISQFTLHAQTQKGNRPSYIKAARPEKA
                     IPLYRHFCQETEKHLKKQIETGEFGADMQVQLINDGPVTIWMDSKVPF"
     gene            3887..4573
                     /gene="pssA"
                     /locus_tag="PKH68_06850"
     CDS             3887..4573
                     /gene="pssA"
                     /locus_tag="PKH68_06850"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNX12693.1"
                     /translation="MIKHIPNTITCLNLFSGCVGVWFAFNGNYEGVALAIIFSAIFDF
                     LDGMAARLLRAYSPMGKELDSLADVVSFGLVPGAMIFHMLSPDFTHNYAFLGFLITIF
                     SALRLAKFNIDERQTSSFIGLPTPANAIFWAGLSLAWSDWLIANPYILMGLTAIFSYL
                     LVAEIPMFSLKFKNLSWKDNRLQFIFLGGAVLLIAFLWKKAIAPLILWYILLSVVLNF
                     MNVEKKTENQ"
     gene            4573..5478
                     /locus_tag="PKH68_06855"
     CDS             4573..5478
                     /locus_tag="PKH68_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX12694.1"
                     /translation="MSIEISHISRSFGEQAVLRDISFQIGEGEVVGFLGPNGAGKTTT
                     MRILAGALDYRSGSAKICGVEVADNPLETKKNVGYLPEQNPLYTDMYVREYLNFVAET
                     YRLGHEKEKRVEELIDLVGLRPEIRKKIGQLSKGYRQRVGLAQALIHNPKVLILDEPT
                     TGLDPNQLVEIRNLIKEIGKNRTVLFSTHILQEIEAVCNRVIIINKGEIVADYTDVNQ
                     ISRYAGNYYQLEVEFLKPLSEEDLKFSAGMHSVETTDNKTFRIIADTDIREKLFDLAV
                     KSGNKILMMKPMEKKMEEIFSELTK"
     gene            5481..6497
                     /gene="ruvB"
                     /locus_tag="PKH68_06860"
     CDS             5481..6497
                     /gene="ruvB"
                     /locus_tag="PKH68_06860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNX12695.1"
                     /translation="MEEPFDIRNNKSTEKELENALRPLSFTDFKGQNKIVENLKIFVQ
                     AARMRTESLDHVLLHGPPGLGKTTLSNIIANELNVGFKITSGPVLDKPGDLAGLLTSL
                     EPNDVLFIDEIHRLSPIVEEYLYSAMEDYRIDIMIDKGPSARSIQLELNPFTLIGATT
                     RSGLLTSPLRARFGINCHFEYYDTIVITGIIERSARLLNVQIDHRAAQEIAGRSRGTP
                     RIANALLRRVRDFAQVKGSGKIDPEIADYALEALNIDKYGLDEVDNKILTTIIDKFQG
                     GPVGLTTIATALSEDPGTLEEVYEPFLIKEGFLKRTPRGREVTELAYKHLGKVKYGDM
                     GTLF"
     gene            6509..6796
                     /locus_tag="PKH68_06865"
     CDS             6509..6796
                     /locus_tag="PKH68_06865"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNX12696.1"
                     /translation="MEKLVNIRTFSQSTDFEMVKSYLESMGIECFGRDEITNRAYLAN
                     VNGGVKLDVLESQAEEAIKLLIAGGYLKPEDFEPTPEFKFVDKILSKFRKK"
BASE COUNT         2025 a         1508 c         1558 g         1775 t
ORIGIN      
        1 tctgtctata attttttaaa tctgccccct aaccccctaa agcctgcctg ccgcaggcag
       61 gggggaatat tgagcgtcta tcatttgttg tgagtttttt aatcatgacc gtgtgtgtca
      121 aaaaaaatta acttggacat gtgtgtttca tctgttttca tattaaggtt taattcttcg
      181 tcggaattca gaaagggtaa tagcagttgc ggtactcaca ttgagcgatt cggacgctga
      241 ggtgtcgggt gaaaaacgga gtatcagcaa tttttcggtc accagagcct ctacggcagc
      301 cgaaatgccg tttccttcat tacccatcac cagtatggca ttgttttcca gtgttttttc
      361 atagatattc gttccatcca taaaggttcc gtacaccggg atttttcctt tcatttgccg
      421 tagaaacgac ggcagatcca cataatgcac atttaccccg gctattgctc ccatggtagc
      481 ctgtatggtt ttcggactgt aagcatcggc acaatgcagg gaacaaaaga catgcctgat
      541 cccgaaccag tcagccagac ggataatggt gcccaggttt ccgggatcct gcacatcgtc
      601 caacgccaga ctgaggctgt tgtggatttc ggcttcattc cacgtatctg caggcttttc
      661 aaaaaccgcc agtacgcctt gtggcgtttt ctgaagggtt atttttttat attcctcttc
      721 tgtaacccgt tctgcttccg ccaccgtgtt ggaatacaga ttaaagacaa aatcaggact
      781 gcaaaaggcc aaaaaccggc attgaagatg cggcaataaa tccgaaacca gtttaattcc
      841 ctctgctacg aagagatttt gctcgtcccg gttttttttg agttccagcg aatgtatcca
      901 ttttattttc gattttgaca gcataatctt ccgtttgttt ttgtccgttt tcatcttcgg
      961 gacacatttt acattacaaa actaataaag aaaacgagaa ttaaggttat atttgcaaaa
     1021 gtttttgatt ttaaccggtt attctgaata ttgagatgag attatcaaag ggttcatttg
     1081 tgcttgctct cgggttgctt gctttggcgt gcaatcccat caagtatgtt cctgataatg
     1141 agcatttgct gaataaggta aaactggaat cggacaataa aacaatttta aaagaagata
     1201 ttgccaccta cgttcgccaa actccgaata atgagatatt tggaatattc aaaatgcagc
     1261 tgggtatcta caacctgtcg gggcccgata ccagcaaatg gataaaccgg cgactgaaaa
     1321 aaatgggcga agaaccggtc atatacaatc cctcgcttac aaccattacc gaaagggaaa
     1381 tctccaaact gttcagcaac aaaggctata tggatgccag cgtgaaaagt gaggttacat
     1441 ttccgaaaga aaaagtggcg gatgttacct acacgatcca gtccaataaa ccatatacac
     1501 tcaacagcta ttcggtcaat atcagtcagc ccgaactggc tgaaattgct tcggacacgg
     1561 ccgagagcct tatcaaaacc ggcaaacttt tcgacagtga tctctttgac caggaaagag
     1621 accgcattgc ttccaaattc agagagctgg gctattataa tttcaccaaa gacctgctgc
     1681 aatattatgc cgacagcacc cagatatcgc accggataga tctggtgctg gaactcaacg
     1741 agcaattgaa aaaagcggat gaggatatca gggaacgggt tttcaggcaa tatacgatca
     1801 ataaagtcca tttttactgt gaaagcgata aaacaacgga tatgcagaac accggcatta
     1861 cgcttgattc tcttacgctg ggtgactata acctggtgta tgaaaacaaa cgcaatatac
     1921 ggccaacggt gctgatagaa catacactca ttatccccgg cagaaggtac agcgaccggg
     1981 cggtggaacg aacctacagc tcgctcaact cactgagtgc ggtgaaatat gtgaatgtaa
     2041 gttaccgtga agtagataaa gacaaactgg attgctatgt ggtaatcact cccaataaac
     2101 tgcaatccat ctcggctgac ggtgaagtca cctattcggc cggttactgg ggatttggag
     2161 gaaatattaa ctacggccac cgcaacgcgt tcaaaggctc cgaaaccgtg tcggtcaagg
     2221 ggcgtatggc ctatgaatat cagggaacgg gacaaaaagc catcgaagtg ggcgccgacg
     2281 ttggattgaa atttccaacc tttttacttc cattctccag cctggatatg cgccggaata
     2341 tacgtgccaa caccgaagtg acggcaacca tgagctaccg cgaccgaccg aaggaataca
     2401 ccggaattgt taccggtgcc ggattcaaat acagctggag tgagaaattt cagattaagc
     2461 acaattttga tgtgctcgat atcagctatg tgaattaccc caaaataacc ggtgaatatc
     2521 aggattatat taattcaaat cctacttttg cctacaactt tgaaccgcac ctgattatgc
     2581 gaaccggtta cagtggctcc tacagtggtt tcacacccac caagcccttc cgcaattata
     2641 caaccatgaa ttatggcgtg gaaaccgccg gtaatattct tcagggagcg aattccctgc
     2701 tcggagctaa acccgactct acaggtgcat tcaaagtctt tggaataaga tatgcacaat
     2761 acgttaaaac cgattttcat atctcctatc accatattta tgatgccaac aatcgcctgg
     2821 tgtatcacgc cgggctgggt atagccatac cttatggaaa tgccgaaaga atgccgttcg
     2881 aaaaacggta tttctccgga ggcgccaaca gtgtaagagg ctggacggct tatcagctgg
     2941 gaccgggaac ctaccgcagt ccgggcaact atgtggatta caacacgcaa atgggtgata
     3001 tcaagctgga tctgaacatg gagtaccgca ccaaactttt ctggaaactg gacggagctt
     3061 tctttctgga tgccggaaat atatggacca tacgcaatta cgtggctcag ccgggtggtg
     3121 tttttcggtt cgataaattt cttggacaac tgggaattgc ctacggaacc ggtatccgcg
     3181 ccgatttttc cttctttgtg ttgcgggtgg acctgggatt gaaactcttc aatccggcgc
     3241 tggaacgaac cgaacaatgg cggacaaaat tcaatgccaa tgattttgcc attaacctgg
     3301 ccatcggtta tccattctga ttatgaagca gatagactta ttataaaata ttgattatga
     3361 gaatattaat tcagcgggta aaagaagctt cagttaccat cggaggccgg ttgaaatcgc
     3421 aaattggggg cggactgctg gtttttgtcg ggattgaaga tgctgatgag gaggacgaca
     3481 gctgccgcct ggcatccaaa ctggtgaaaa tgcggatttt taacgacgaa aatggggtga
     3541 tgaatttacc ggtaaccgaa atagatggcg aaataatgat aatcagtcag tttacccttc
     3601 atgcacaaac ccagaagggc aaccggccat cgtacatcaa agcggcgagg cccgaaaaag
     3661 caataccgct gtaccggcat ttttgccagg aaacagaaaa acacctgaaa aaacagatcg
     3721 aaaccggtga atttggagcc gatatgcagg tgcaacttat caacgatggg cctgtaacca
     3781 tctggatgga cagtaaagtg cctttctaaa aaatatattt ctatgatttt caatcgatta
     3841 taactgattt tcaacttttt caacaacaaa aacgggaggt attaacatga taaaacatat
     3901 tccaaatact ataacctgtc taaatctctt ttcgggttgc gtgggcgttt ggttcgcctt
     3961 taacggaaat tatgaaggtg tagctcttgc cattattttt tcggcaatat tcgattttct
     4021 ggatggaatg gctgcccgtc tgctaagagc ctattcaccc atgggaaaag agttggattc
     4081 actcgccgat gtggttagtt ttggactggt gcccggagcc atgatttttc acatgttatc
     4141 acccgatttt acccataatt atgcatttct gggctttctt atcaccattt tttctgcatt
     4201 gcgactggct aaattcaaca ttgacgaacg tcagacaagc tccttcattg gattgccaac
     4261 ccctgccaat gccattttct gggccggctt atcactcgcc tggtccgact ggttgatagc
     4321 aaatccatat attttaatgg gcctgacggc tatattctct tatctgctgg tagccgaaat
     4381 tccgatgttt tccctcaaat tcaaaaacct gagctggaaa gacaaccgct tacaattcat
     4441 atttctgggt ggtgcggtgc tgttgattgc atttctgtgg aaaaaagcca tagcccctct
     4501 tattttatgg tatattcttc tttcggtggt tttgaatttc atgaacgtag aaaaaaaaac
     4561 tgaaaatcag taatgtcgat cgaaatttca catatttcgc gatctttcgg agagcaagcg
     4621 gttctccggg atatcagttt tcagataggt gaaggggaag tggtgggttt tctgggaccc
     4681 aacggagccg gaaaaaccac caccatgcgc atacttgccg gagcacttga ttaccggagc
     4741 ggcagtgcta aaatttgcgg cgtggaagtg gctgataacc ccctcgaaac caagaaaaat
     4801 gtaggttatc tgcccgaaca aaatccgctc tacaccgata tgtatgtgag ggaatacctt
     4861 aactttgtgg ccgaaacata ccggttgggc catgaaaagg aaaaacgggt ggaagaattg
     4921 attgacctgg tggggctgcg acccgaaatc agaaaaaaaa tcggacagct gtcgaaagga
     4981 taccgccaac gggtgggact ggctcaggca ttgattcata atccgaaagt gctgatactg
     5041 gacgaaccta ccacaggact tgatcccaat cagttggtcg aaatccgcaa tctgattaaa
     5101 gaaatcggga aaaaccggac cgtactcttc agcacacaca tcctgcagga aattgaagcc
     5161 gtttgcaacc gggtgataat catcaataaa ggcgaaatag tagccgatta taccgatgtc
     5221 aaccagattt cccggtatgc cggaaattac tatcaattgg aagtggaatt tcttaaaccg
     5281 ttgtccgaag aagatttaaa gttttcagca ggaatgcatt cggtggaaac aaccgacaat
     5341 aaaacattca gaattatagc cgataccgat attcgggaaa aattattcga tttggccgtc
     5401 aaatccggca acaaaatact gatgatgaaa ccgatggaga aaaaaatgga agaaatattc
     5461 agcgaactga caaaataatt atggaagaac catttgacat acgaaataat aaaagtaccg
     5521 aaaaagaact cgaaaacgcc ttgcggccac tcagttttac tgattttaag ggtcagaaca
     5581 aaattgtgga aaatctgaaa attttcgttc aggctgcccg catgcgtacc gaatcgctgg
     5641 atcatgtact gcttcacggt cctccgggac tgggcaaaac cacgctttcc aatatcattg
     5701 ccaacgaact gaatgtgggc tttaaaatca cgtcgggacc ggtgctcgac aaacccggtg
     5761 atctggctgg actgcttacc agcctggagc caaacgatgt cctttttatt gacgaaattc
     5821 accggttgag tcccattgtg gaagaatacc tctattcggc catggaagat taccggattg
     5881 atatcatgat agataaaggg ccgagtgcac gatccatcca gctggaatta aatccgttta
     5941 cactcattgg tgccacaacc cgcagtggtc tccttaccag tccgctacgt gcccggtttg
     6001 gcattaactg ccattttgaa tattacgata cgattgtaat tacgggtatc atcgaacgct
     6061 cggcaagatt gctgaatgtt cagattgatc accgggctgc tcaggaaata gccggacgca
     6121 gccggggtac accgcgtata gccaacgccc tgctccggcg ggtacgcgac tttgctcagg
     6181 taaaagggag cggaaaaatt gaccccgaaa tagccgacta tgcactggaa gcattaaaca
     6241 tagataaata cgggctggac gaagtggata acaaaatact cacaaccatc atcgataaat
     6301 ttcagggtgg acctgtaggg cttaccacta ttgcgacagc tctcagtgaa gaccccggca
     6361 cgctggaaga ggtctatgaa ccttttctta tcaaggaagg ttttctgaaa cgcacaccgc
     6421 gcggacggga agttaccgag ctggcttaca aacacctggg taaagtgaaa tacggagata
     6481 tgggaacatt attttaaaaa acatcattat ggaaaaattg gtaaatatcc ggactttcag
     6541 tcagtccacc gattttgaaa tggttaaatc ttatctcgaa tcgatgggta tcgaatgttt
     6601 cgggagagat gagatcacca accgcgccta tcttgccaat gtgaacgggg gcgtaaaact
     6661 cgatgtgctc gaatctcagg ccgaagaggc cattaaactt ttaatagcag gcggatacct
     6721 gaaacccgaa gatttcgaac caacgcctga attcaaattc gttgacaaaa ttctgagcaa
     6781 attcagaaaa aaatagtttg aatatacata aatacaaaaa agccaattaa tttatggcaa
     6841 aagaaatgaa atcgctggcg aaggac
//
LOCUS       DAOPZK010000103         6860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_13492_length_6860_cov_24.950625, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000103 DAOPZK010000000
VERSION     DAOPZK010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13492_length_6860_cov_24.950625"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKH68_06870"
     CDS             <1..357
                     /locus_tag="PKH68_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNX12697.1"
                     /translation="YCERRKVGALLVKNKMIISDGYNGTPSGFENKCEDENNVSKAYV
                     LHAEANAISKVARSHNSSDNATLYVTASPCMECSKLIIQAGIKRVVYGEKYRIMDGVE
                     LLERAGIEVVYLDNKE"
     gene            382..>1848
                     /locus_tag="PKH68_06875"
     CDS             382..>1848
                     /locus_tag="PKH68_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNX12698.1"
                     /translation="MNKKNILIIITVVVAVLFGLFFGNLIALKANRGNKIKNLTEYFS
                     GSNSKVDEMLSLIDEQYVDTINIDSITDEVMGDLVSKLDPHSVYIPAKDLEAVNSELE
                     GSFSGIGVQFNIQNDTVMIVSVISGGPSEKVGLMAGDRIVKVNDTIFAGKNVVSNEKV
                     MKKLRGPKGTKVKLSVKRHGTKELLSYAITRGDIPVNSVAVAYIIEPGVGFIGLNNNF
                     GKNTYDEFLHAIAELRAKGAKKYIIDLRENSGGLMDVAIRMINEFLPKGQMIVYSEGK
                     AYPRYEAKADGSGSCINNELVVLIDEFSASASEIFAGAIQDNDRGLIIGRRSFGKGLV
                     QQQIPFSDGSAVRLTVARYYTPSGRSIQKPYVKGDAKAYEEDIINRFNHGEFDSKDSV
                     HVANTLKYRTLKGRTVYGGGGIMPDIFVARDTSKYTPYLTKVINYGYIYQFAFQYTDR
                     NRTKLNGFKTWQDMDNYLTAENLLNEFTTFAAAKGVAPN"
     assembly_gap    1849..1948
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1949..2659)
                     /locus_tag="PKH68_06880"
     CDS             complement(<1949..2659)
                     /locus_tag="PKH68_06880"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR01768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HNX12699.1"
                     /translation="MIHIYDNICTNAGRGKKMLAVLLDPDKCSGSVLADTLAEFEKCT
                     PDFIFIGGSSGMCNSDDLLQSLQHIPSPKILFPGDASQFNPGADGLLFLSLISGRNAD
                     YLIGQHVQSAIEIKKSNVEVIPTGYILIDGGNHSAVQRVSNTTPIGADDIDECISTAV
                     AGELLGMKLIYLEAGSGAAVAVSAEMISAVKQQLNIPLIVGGGIKTTEQLISALNAGA
                     DLIVVGNIFETETGKIAEF"
     gene            complement(2656..3300)
                     /locus_tag="PKH68_06885"
     CDS             complement(2656..3300)
                     /locus_tag="PKH68_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNX12700.1"
                     /translation="MINYQVVSHKDACICLFENKFSSDELLREFEHAGLYSHELDNLK
                     TENRKKEFLGLRLALKHCLKGEEKAIGHTQKGKPVLEDKSYNISFSHCRGWIAVITHR
                     GLEVGIDIENPTQKLNSLYKRFLCEEELQEYLKDENFDYLRIIWSAKEALYKIIGDEA
                     VNFKNQLHILPFRVEREGDITGIHNPTGRTYDIHYQLTDSYTLAWCIDQKNRQQ"
     gene            complement(3310..3897)
                     /gene="gldD"
                     /locus_tag="PKH68_06890"
     CDS             complement(3310..3897)
                     /gene="gldD"
                     /locus_tag="PKH68_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNX12701.1"
                     /translation="MTKPKIHLLFIVVLFVSEIFTGCGDVIVPKPYGYFRVDLPAHRY
                     ERLDSAPLPYSFEKSVYARVIPRAENGENFWIDLKYPALNASLFCSYKPVRSNLQELT
                     EDTRKIVYKHTVRADGIGEKVFVNPGKKVYGVLYDLTGNTASSVQFVLTDSTRHFFRG
                     ALYFENVPNKDSIAPMSAYIREDVIRLMESFEWKK"
     gene            complement(3890..5203)
                     /gene="gldE"
                     /locus_tag="PKH68_06895"
     CDS             complement(3890..5203)
                     /gene="gldE"
                     /locus_tag="PKH68_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNX12702.1"
                     /translation="MLNNILVSPFTVTSGILLFCGLIFLCITAFFSGAEVAFFSLDQR
                     TIDEFDESEDKSDKAAIRLLGTPQKLLATILIANSITNIAVIVCLVNFTLSVFDFSFV
                     PGLGFIIQVAVIGFLILIFGEIMPKVFSSEYAKVTVQRSARIILSLQNFFNPFVELFA
                     NSRALVNSKLTKRSYNNISMDELSQAFDLTTETPDEDKEILEGIIKFGNIQVADIMTS
                     RVDIVDVDIKLNYKQLLDLIIKSGYSRMPIYSGSRDNIKGILFSKDLLPHLDKPSTFR
                     WQTLIRPPYYVPETKKIDDLLNEFQENRVHLAIVVDEYGGTSGLVTLEDILEEIVGDI
                     SDEYDEEETLFTKIDNHTFLFEAKILLNDFFKIAEISKEDFLKKTEDVETLAGLILEL
                     KGEMPKKNERIGYGRYVFEVTSVDNRRIKKVKLYIKSDPNKSLND"
     gene            5439..6116
                     /gene="trmD"
                     /locus_tag="PKH68_06900"
     CDS             5439..6116
                     /gene="trmD"
                     /locus_tag="PKH68_06900"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNX12703.1"
                     /translation="MRIDIISAVPELLESPLNYSIIKRARDKNLVEIHVHNLRDYTHD
                     KHRRVDDYAFGFGAGMVLQIEPVDNVISKLKAERKYDEIIYTSPDGEKFDQKLANRFS
                     MAENLMILCGHYKGIDHRIREHLITREISIGDFVLTGGEMPAALMTDAIVRLIPGAIG
                     DETSALSDSFQDNLLAPPVYTRPAEYKGWKVPDILLSGHQAKVEEWLHQQSVERTKRL
                     RPDLLEE"
     gene            6118..6384
                     /locus_tag="PKH68_06905"
     CDS             6118..6384
                     /locus_tag="PKH68_06905"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HNX12704.1"
                     /translation="MMNALYLYLVIVNVATAALFTYDKVAARRGKRRIPELILHGFEL
                     AGGVFSVVVLMYVINHKSRKMSYYLKTYLFLILWAAAIWFIIKY"
     gene            6511..6837
                     /locus_tag="PKH68_06910"
     CDS             6511..6837
                     /locus_tag="PKH68_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNX12705.1"
                     /translation="MAWIFLIIGGLFEVGFTTCLNAAKHSTGAVATLWYAGFAVSLGL
                     SMIFLMMATKSLPMGTAYAVWTGIGAVGTALIGMLIFKEPADFWRVFFIFTLIASIVG
                     LKYVAP"
BASE COUNT         1896 a         1399 c         1550 g         1915 t
ORIGIN      
        1 tactgcgaac gccgcaaggt aggcgctttg ctggtgaaga acaagatgat catttctgat
       61 ggttacaacg gcactccatc gggttttgag aataaatgtg aagatgaaaa caatgtttcg
      121 aaagcctatg tgcttcatgc cgaagccaat gctatctcta aagttgcccg ttcgcacaac
      181 agcagcgaca atgctacgtt atacgttacg gcttcgccct gcatggaatg ttcgaaactg
      241 attattcagg caggcatcaa acgggtggtt tacggagaaa aatacagaat catggacggt
      301 gtggaattgt tggaacgtgc cggaattgaa gtggtttatc ttgataataa agaataaaaa
      361 aattaactga acttacaata aatgaataaa aaaaacatcc tcataataat tacggtagta
      421 gttgctgtac tttttgggct atttttcggt aatctgattg ctttaaaagc aaaccggggg
      481 aacaaaatta aaaatctaac agagtatttt tcaggaagca acagtaaagt tgatgaaatg
      541 ctctcattga tcgacgagca gtatgtcgat acgattaaca tcgactccat caccgatgaa
      601 gtgatgggcg acctggtatc aaaactcgac ccgcattcgg tctatattcc tgccaaagac
      661 ctcgaagccg tcaacagtga gctggaaggc agtttcagcg gtattggcgt gcagtttaat
      721 attcagaacg atacagtgat gattgtgtcg gtaatcagtg gcggaccttc cgaaaaagtg
      781 ggactgatgg ccggtgaccg cattgtaaag gtaaatgata ccatttttgc cgggaaaaat
      841 gtggtgagca acgaaaaggt gatgaaaaaa cttcgcggac cgaaaggtac gaaagtgaaa
      901 ctgagtgtaa aacgtcacgg aacgaaagaa ctactgtcct acgccattac aaggggcgat
      961 attccggtta attcggttgc ggttgcttat atcatcgagc cgggagtggg cttcattgga
     1021 cttaacaata actttggaaa aaatacctat gacgaatttc tgcatgccat tgccgaactt
     1081 cgtgccaaag gagcaaaaaa atacattatc gacctgcgcg aaaacagcgg tggtctcatg
     1141 gatgtagcta tccggatgat caacgaattt ctgcccaaag ggcaaatgat tgtttattcc
     1201 gaagggaaag cctatccgcg ctacgaagcc aaagccgatg gaagcggaag ctgtatcaac
     1261 aacgaattgg tggtattgat tgacgaattc agcgcttccg ccagtgagat ttttgccggt
     1321 gccatacagg ataatgaccg gggtctgatt atcggacgcc gttcgtttgg caaagggctg
     1381 gttcagcagc aaattccctt ctccgatggt tcagcagttc gtctcaccgt tgcccggtac
     1441 tatacaccct cgggccgaag catccagaaa ccctatgtga aaggcgatgc caaagcgtat
     1501 gaagaagata tcattaaccg cttcaatcac ggtgagttcg acagtaaaga cagcgtacat
     1561 gtagccaaca ccctgaaata cagaacattg aaaggtcgta cggtgtacgg tggcggtggt
     1621 atcatgcccg atatttttgt tgcccgcgat acttcaaaat acacccccta tctgacaaaa
     1681 gttatcaatt acggatatat atatcagttt gcttttcagt acaccgaccg aaacagaacg
     1741 aaactgaacg gcttcaaaac ctggcaggat atggataact acctgactgc tgagaatttg
     1801 ctcaacgagt ttacaacttt tgctgctgcc aaaggcgttg ctcctaatnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa actccgctat ttttcctgtc tcagtttcga
     1981 agatattgcc tacgacaatc agatctgctc cggcatttaa tgccgagatt agttgctctg
     2041 ttgttttgat accaccaccc acaatgagtg gaatattaag ctgttgcttt acagctgata
     2101 tcatttctgc tgaaaccgct acggcagcac cgcttcctgc ttcaagatag atcagcttca
     2161 tgccaagcaa ttcgccggca acggccgttg aaatacattc atcgatatca tctgccccta
     2221 tcggagttgt attgctaaca cgctgtactg cagaatgatt tcctccatca atcagaatat
     2281 aaccggtagg aataacttcc acgtttgatt tttttatttc aatagccgat tgtacatgct
     2341 gtccgatcag ataatcagca tttcgccctg aaattagcga aagaaaaagc aatccgtcag
     2401 ctccggggtt aaactgagag gcatcgcccg gaaagagtat tttgggtgac ggaatgtgtt
     2461 gaagggattg aagcaaatca tcggagttgc acatccccga actgccgcca atgaaaatga
     2521 aatcgggagt gcatttttca aattccgcca gcgtgtccgc cagcacggag ccactgcatt
     2581 tgtcgggatc gagcagaacg gcaagcattt tcttacccct gcctgcatta gtacagatat
     2641 tatcgtaaat atggatcatt gctgccggtt tttttgatca atacaccagg caagggtata
     2701 gctgtcagtt agttgatagt gaatgtcata cgttctgcct gtcggattgt gtatcccggt
     2761 aatatcacct tcacgctcca ccctgaaggg taaaatatgc agttgatttt tgaaattgac
     2821 agcttcgtcg ccaataattt tataaagtgc ctccttggcc gaccagatga tgcgcagata
     2881 atcaaagttt tcgtccttca ggtattcctg cagttcttct tcgcaaagaa aacgtttgta
     2941 tagcgaattg agcttttgag ttggattttc gatatcaatt cccacttcga ggccccggtg
     3001 agtgatgact gcaatccatc cgcggcaatg agagaaactg atgttataac ttttatcctc
     3061 caacaccggt ttaccttttt gggtatgacc aattgccttt tcctcccctt tcaggcaatg
     3121 tttcaatgcc agccgaagtc ccagaaattc ctttttccgg ttctcggttt tgaggttgtc
     3181 gagctcatgg ctgtaaagcc ctgcatgttc gaattccctc aataattcgt ccgatgaaaa
     3241 tttattttca aacagacaaa tacaagcatc tttatgtgaa acaacctgat aattaatcat
     3301 tctaaaaaat tattttttcc attcgaaact ttccatcagt cggatgacgt cttcccggat
     3361 ataggccgac atgggagcta tagagtcttt atttggtaca ttttcaaaat agagagcacc
     3421 cctgaaaaaa tgccgggtac tgtcggtcag aacaaactga acggaagaag cagtatttcc
     3481 cgtcaggtcg taaagaacgc catacacctt cttcccggga tttacaaaaa ccttttcacc
     3541 aattccatcc gccctgacgg tgtgtttgta cactattttg cgggtatctt ccgtgagttc
     3601 ctgaagattg gaacgtacgg gcttgtaact gcaaaacagg ctggcattga gcgcaggata
     3661 tttcagatca atccagaaat tttcaccatt ttcagcacgg ggaattaccc gcgcatagac
     3721 tgacttttcg aatgagtagg gtagcggagc cgaatcgagc ctttcatacc ggtgtgcagg
     3781 taaatctacc cggaaatatc cgtatggttt gggtacgatg acatcgccgc aaccggtgaa
     3841 aatttcagac acaaacagca caacgatgaa taataaatgg attttcggtt tagtcattca
     3901 ggctcttgtt tgggtcactt ttaatgtaaa gttttacttt tttaatgcgg cggttgtcca
     3961 ccgaagtgac ttcgaataca taccggccgt agccaattcg ttcattcttt ttaggcattt
     4021 cacctttgag ttccagtatc agtcctgcaa gtgtttctac gtcttcggtt tttttcagaa
     4081 aatcctcttt tgatatttcg gctattttga aaaaatcatt caacaaaatc tttgcttcaa
     4141 aaaggaaggt gtggttgtca attttggtga agagtgtttc ttcttcatcg tactcgtcgc
     4201 tgatgtcgcc tacaatttct tccagtatat cttccagcgt tacaagtccg gaggtgccgc
     4261 cgtattcatc cactacgatg gccagatgca cccggttttc ctgaaactca ttcagcaagt
     4321 cgtcgatttt ttttgtttcg ggcacatagt agggtggtcg tatcagtgtc tgccagcgaa
     4381 aggtggaggg tttgtccagg tgaggcaaca gatccttact gaaaagaatg cctttgatat
     4441 tgtcgcgcga accggaataa atcggcattc ttgagtagcc ggatttaata atcagatcga
     4501 gcaattgttt gtaattcagt ttgatatcca catccacaat atcaacccgc gaagtcatga
     4561 tatcggctac ctgaatattc ccgaatttga taattccttc cagtatttcc ttgtcctcat
     4621 cgggtgtttc ggtcgtgagg tcgaatgcct gcgacaactc atccatcgaa atattgttgt
     4681 aactccgttt ggtgagtttg ctgttgacta atgcgcgtga attggcgaat aattcgacaa
     4741 agggattgaa aaaattctgc agactcagga tgatgcgggc actgcgttgt acagttactt
     4801 tggcatattc cgatgagaaa accttgggca ttatttcccc gaaaatcaaa atcagaaaac
     4861 cgatgacagc aacctgtatg ataaatccca ggccgggaac aaacgaaaaa tcgaacaccg
     4921 aaagggtgaa gttgaccaga caaacgataa ccgcaatatt ggtaatgctg ttggctataa
     4981 ggatggtggc taatagcttt tgcggcgtgc cgagtaaacg gatggctgct ttgtcgcttt
     5041 tatcttcact ttcgtcgaac tcgtcgatgg ttcgctgatc gagcgagaag aatgccacct
     5101 ctgcgcccga aaagaaggcc gtgatacaaa gaaatataag cccgcaaaag agcagtatgc
     5161 cgctggtaac ggtaaatgga ctgacaagaa tattgttgag cattatgttc cgaaaatttg
     5221 tttccgataa tttaaaatgg aagttgagat tatctaattg aatgcaaata tacggcataa
     5281 attttatttt tttgtagtaa ggtaataaaa atcagattac aggagattaa ataaaatcac
     5341 ggtattattg aaaatccgtc ttttttctgg tttttagaat tagcttcaat tctgtaatgg
     5401 aacactattt ttttgtactt ttgtaaaaac tttatcccat gcgaatagat ataatttctg
     5461 ccgtacccga attgctcgaa agcccgctta attactccat tatcaagcgt gcgcgcgata
     5521 aaaatctggt ggaaatccat gtgcacaacc tgcgggatta tacccacgac aaacaccggc
     5581 gtgtggatga ttatgctttt gggtttgggg caggcatggt gctgcagatc gaaccggtgg
     5641 ataatgtgat atcgaaattg aaagctgagc ggaagtacga tgaaattatt tatacctcgc
     5701 ccgatgggga gaaatttgat cagaaactgg ccaaccgctt ttccatggcc gaaaatctga
     5761 tgatactttg cggacattac aaaggcattg accaccgtat tcgggaacat ttaattaccc
     5821 gggagatcag tattggcgat tttgtgttga cagggggtga aatgcctgcc gcgttaatga
     5881 ctgatgccat cgtgcggttg atacccggag ccattggcga cgaaacttcg gcattgtccg
     5941 atagttttca ggataatctg ctggcacctc cggtttacac ccgtccggct gaatacaagg
     6001 gctggaaagt tcccgatatt ctgctctccg ggcatcaggc taaggtagaa gaatggctgc
     6061 atcagcagtc ggtggagcgg acaaaacgcc tcagaccgga tttattggaa gaatgacatg
     6121 atgaatgcat tatatctata tctggtgatt gtaaatgtgg cgacagcagc tttgtttact
     6181 tacgacaaag tggcagcccg ccggggcaaa aggcgtattc cggagctgat ccttcatggt
     6241 tttgagttgg ccggcggcgt gttcagtgta gtggtgctga tgtatgtaat caatcacaaa
     6301 tcgcgtaaaa tgagttatta cctgaaaaca tacctgttcc tgattttgtg ggctgcagcg
     6361 atttggttta taattaaata ctgatgtaat ttccagggaa aaatatttcc aaaaaacaac
     6421 tatacatcag taaatcagat atataaagaa gttaaatatt gtaattccaa aattggttaa
     6481 atagtaaata gcaaaatagt aaataaaaaa atggcctgga tttttttgat aatcggcggt
     6541 ttgtttgagg tgggttttac tacctgcctg aatgccgcaa agcacagcac cggagccgtt
     6601 gcaactctct ggtatgcggg gtttgcggta agtctggggt tgagtatgat ttttctgatg
     6661 atggccacca aatcattgcc catgggaacg gcttatgctg tctggacggg aattggcgct
     6721 gtgggaacgg cactgattgg tatgttgatt ttcaaagaac cggcagattt ttggcgtgtg
     6781 ttctttattt ttacgcttat tgcctctatt gtgggcctga agtatgtagc gccttaaata
     6841 taatcttttt tacatttagg
//
LOCUS       DAOPZK010000104         6780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_13699_length_6780_cov_20.894572, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000104 DAOPZK010000000
VERSION     DAOPZK010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13699_length_6780_cov_20.894572"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1644)
                     /locus_tag="PKH68_06915"
     CDS             complement(172..1644)
                     /locus_tag="PKH68_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNX12706.1"
                     /translation="MIKSEINYKLVSKVIGSLLLVESATLFFVLFISLIYGENDGLYF
                     VGTSIFALIVGWLMILYGKNAPERIGKREGSMIVTFTWIFFSLIGALPYWLSKSIPSF
                     TDAFFETISGFTTTGASILNNIEELSHGMLFWRSLTHWVGGLGIIVISLALLPMFGVS
                     GTQLFAAETSGPTKDKIHPKLNETAKRLFLIYVILTAFETVFLLFGRMNLFDAVCHSF
                     STIATGGFSTKQASIAYYQSPYIEYVISIFMILSGVNFGNYYLAYQRKYYKIKENEEL
                     RYYLIVLFVFTVIVAFSLVDFSEIITAQSVEKAWRDALFTVSSLMSTTGMVTADYMYW
                     KPLTWIILITIMMTGASAGSTSGGIKMIRVVISAKACYYEFKRLIHPTAVIPVRYNKH
                     VVREDILTRVIAFTLLYIVFALFGILVLAISGMGFIESIGGMVTCLGGVGPGLGLIGP
                     MGNFSQIPEFSKWFLDLVMLTGRLELYTVLMLFTPAFWRG"
     gene            complement(1669..3114)
                     /locus_tag="PKH68_06920"
     CDS             complement(1669..3114)
                     /locus_tag="PKH68_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNX12707.1"
                     /translation="MILRILGGLLLVEGVFLLFPVFVALLYREHDIRYYLYTIFIAVG
                     MGSIALLIGRKAENVFGKREGAVIVTVTWLMYTVIGTLPLWLSGNIPAFTDAFFESLS
                     GFTTTGTSILNNIEALPYNMLFWRSLTHWIGGLGIVAMFLAIMPMLGGAGYELFSSES
                     TTPIKEKILPKISQTAKILLLIYVVLTMLCAFSLKIAGMDWFDAINHALSTISTGGFS
                     TKQLSIAYWNSPAIEYVLIFFMFFSGVNFIIFYYLYKFNWVKIRKNEELKYYIVILLA
                     SVLVIAYSQIDFSRTLTLSGIEKSLRGSLFIVTSCMTTTGLCTDDYTLWNSYTWIILL
                     VLMVSGASTYSTSGGIKVARVVLLTKFCYFEFKKLIHPNAIIPVRFNGFLVRDDLMMR
                     VLAFTVIYLLIIVAGSFVLSLTGLGFEEAISGMITCMSDVGLGLGKLGPSSSFAEIPV
                     VSKWFLAFVMLVGRLEIYSVLLLFTPEFWKK"
     gene            complement(3194..4528)
                     /gene="trkA"
                     /locus_tag="PKH68_06925"
     CDS             complement(3194..4528)
                     /gene="trkA"
                     /locus_tag="PKH68_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNX12708.1"
                     /translation="MKIIIAGAGEVGTHLAKLLARESLDITLMDEDPEKMSNLEANYD
                     LLTRVGLPTSIASLKECGIEGTDLFIAVTPFESVNMTACMLATNLGAKKTLARIENYE
                     YLQPGNKDFFEKLGVDYLICPEKLAAEEIVEALKHPWLRQNLSFCNDALILLGMKIRE
                     NSEVLRKPFNSGYFDHGKYRVVAINRKEKTIIPSGYDEIQAGDIVYFITMKQNVDFVR
                     EQAGKALFQVKNAMIMGGSRIAQKTIQYLPNNINTKMLERDKEKCYFLAERLNNTLII
                     NADGRNVDALRSEGIQEIDAFVATTANSEANILACLEAKRLGVKKTVAEVENIDYIQL
                     AEDLDIGTVINKKMIAASYIYQLTLDADVLEVRSLTSADAEVVEFVAKEGSKITKSQI
                     KNIRLPENVNIGGIVRDGVGSIVYGNTQILPNDHVIVFCISSAIRKVETLFN"
     gene            complement(4533..6473)
                     /gene="dxs"
                     /locus_tag="PKH68_06930"
     CDS             complement(4533..6473)
                     /gene="dxs"
                     /locus_tag="PKH68_06930"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNX12709.1"
                     /translation="MIERKYKYLNNINSSNDLKTIKRSELGVVCQELRDFIIDEVSQN
                     PGHLGSSLGVVELTVALHYVFNTPYDRIVWDVGHQAYGHKILTGRRDVFHTNRKLGGI
                     SGFPKIEESEYDAFGVGHASTSISAALGMAVSAQMKGEDRKVVAVIGDGAMTGGLAFE
                     GLNNTSMDKNDLLIILNDNQMAIDPISGGFTQYLVDITTSRLYNKMRYKAYSFFKKRH
                     WMDEERKKQIIRVNNSLKTLFTRQKQNIFEAMNIRYFGPVDGHDVDNLIRILTEIKNY
                     KGPKVLHLITKKGRGYEPAEKSATVWHAPGKFDVETGERLVCNHGNVEPPLFQDVFGK
                     TLLELAKENKNIIGVTPAMPSGCSMIIMQKRFPDRVFDVGIAEGHAVTFSAGLAKEGM
                     VPFCNIYSSFMQRAYDNVIHDVALQNLPVIMCLDRAGIVGSDGATHQGEFDLAYMRCI
                     PNLTIAAPRNETELRHLMYTAQLPGKGPFVIRYPRGKGTIIDWHQPWKELTIGKGECL
                     KEGNDLAIITIGTMATPAEKAINIIENERDVTIAHYDARFVKPLDQELLHSIGKKFKY
                     IITVEDGVIDGGFGSAVLEFMADNNYQMNITRLGIPDSFVEHGTPEELYAKLGLHAEG
                     LRVSFEAVLIEELEKKEIIKKK"
BASE COUNT         2123 a         1475 c         1241 g         1941 t
ORIGIN      
        1 ttgaattgaa caaaataaat gaaagaaaaa tttgaaatta tttttgagaa aaaattaaat
       61 ctctaataaa tagcatgtta gtagtgcaaa aatttgacta aaaaaacaag aaggtaatct
      121 taacactggt aagaatataa accagagtaa atactataat attaattgaa attatccgcg
      181 ccagaaagca ggcgtgaaaa gcatcagtac tgtataaagt tccaacctgc cagtgagcat
      241 cactaaatca agaaaccatt tactgaattc gggtatttga gaaaaattac ccatggggcc
      301 aatcaatccc aaaccgggac ctacgccccc gaggcatgtt accatacctc cgattgattc
      361 gataaaaccc atgccggaaa tggccagcac cagaatacca aacaaggcaa agacaatata
      421 aagcagtgtg aatgctataa cccgtgttaa tatatcttca cgcacaacat gtttgttgta
      481 tcgcaccgga atgacagctg tagggtgtat cagccttttg aattcgtagt agcaagcttt
      541 ggcagaaata actacgcgga tcattttgat gcctccactt gtagaaccag ctgaggcacc
      601 tgtcatcatt atagtgatta aaattatcca tgtcaatggt ttccagtaca tataatcagc
      661 agtaaccata ccggtggtag acatgagcga tgaaacagtg aacagggcat cacgccaggc
      721 tttctcgaca gattgagctg tgatgatttc cgaaaaatcg accaacgaaa atgcgactat
      781 taccgtaaaa acaaaaagaa caatcagata ataacgcaat tcctcatttt ctttaatctt
      841 ataatattta cgttggtatg ccaggtagta attaccgaaa ttaacaccgg aaagtatcat
      901 aaatatggag ataacatatt caatataagg tgattgataa taagcaatgc tggcctgttt
      961 ggttgaaaat cctcctgtgg caatggtaga aaatgaatga caaacggcat caaacaaatt
     1021 catcctgcca aataatagaa aaaccgtttc aaaggcagta agaattacat atattaaaaa
     1081 taagcgttta gccgtttcgt tcaatttagg atgtatttta tctttggtag ggcctgaagt
     1141 ttcagcggca aaaagctgcg ttccgcttac tccgaacata ggaagtaacg ccagtgatat
     1201 gacaataatt ccaagtccac ctacccaatg agtaagtgaa cgccaaaaaa gcataccgtg
     1261 cgaaagttct tcaatattat taagtattga tgcacccgtt gtagtgaaac cagagatcgt
     1321 ttcaaaaaaa gcgtccgtaa acgatggtat acttttactg agccaatacg gtaatgctcc
     1381 tatcagtgaa aagaaaatcc atgtgaaggt aactatcata gagccttcgc gcttaccaat
     1441 cctttcgggt gcatttttcc catacaaaat catgagccaa cccactataa gagcaaagat
     1501 tgaagtacca acaaaataaa ggccatcatt ttcaccataa atgagggaaa taaacagcac
     1561 aaaaaacagg gttgcacttt cgaccagcaa caggctacca attaccttcg aaactaattt
     1621 ataattgatt tcagatttta tcatacactt aaaaggttta ttctgaattt attttttcca
     1681 gaattcgggt gtaaaaagca ataaaacaga gtaaatttcc aaacgtccca cgagcatcac
     1741 aaaagccaga aaccatttgc tgacgacagg aatttcagca aaactggagg aagggccaag
     1801 tttgcccagt cctaaaccca catcgctcat acaggttatc attccgctga tggcttcttc
     1861 gaaacctaaa ccggtgagac ttaatacaaa ggaaccagcc acaataatga gcagataaat
     1921 aacagtgaaa gcaaggacac gcatcattag atcatccctg accagaaagc cattgaaccg
     1981 tacaggtatt attgcattgg gatgaattaa ttttttgaat tcaaaataac aaaatttggt
     2041 aagcaacacc acgcgagcca ccttgatccc tcctgaggtg gaataggtag atgctccgga
     2101 aaccatcaaa accagcaaaa ttatccaggt atatgaattc caaagcgtat aatcgtcggt
     2161 acaaagcccc gtagtggtca tacacgatgt aactataaac aaacttcccc ttaaggactt
     2221 ttcaatacct gacagtgtta acgttcggct aaaatctatc tgcgagtaag ctataaccaa
     2281 cacagatgcc agcaaaatga ctatataata cttgagttcc tcgttttttc tgattttgac
     2341 ccagttaaat ttatacagat aataaaatat tataaaattt accccggaaa aaaacataaa
     2401 aaaaataaga acatattcta tcgccggaga attccagtag gcaatgctca gttgtttggt
     2461 agaaaaacct ccagtgctga tggtagacaa ggcatggtta atagcatcaa accagtccat
     2521 tcctgctatt ttcagggaaa aagcgcatag catggtaagt accacgtaaa taagcaatag
     2581 aattttggct gtctggctta tttttggtag aattttctct ttgatgggag tggtgctttc
     2641 cgacgaaaaa agttcgtatc ctgcaccacc caacatcggc ataatggcca gaaacatggc
     2701 aacaattccc aatccaccta tccagtgagt aaggcttcgc cagaaaagca tattatatgg
     2761 taaagcctca atattattaa gaatggaagt gccggtggtg gtaaaaccgg acaatgactc
     2821 aaaaaaggca tcggtaaatg caggaatatt gccactcagc caaagaggca gtgtaccgat
     2881 aacggtatac atcagccacg taacagtaac aatgacagca ccttcgcgtt tcccgaaaac
     2941 attttcggct ttacgtccaa tcaacaatgc aatcgagccc ataccaacag ctatgaatat
     3001 ggtatacagg taataccgga tatcatgctc acgataaagg agtgccacga acacgggaaa
     3061 aagcaggaat accccttcga caagaagaag tccgcccaat attcttaaaa tcaatttgat
     3121 atttaaatcc ctgttcatta atagcgggtg gtaaatgtca atttaagcaa cttacgacga
     3181 ttatatttct atactaatta aataaggttt ccactttacg aatagctgac gatatgcaaa
     3241 acacaatgac gtggtcattg ggcaaaatct gtgtattacc ataaacgata ctccccactc
     3301 catcacgtac gatgccaccg atattgacat tctcggggag tcggatgttt ttaatttgag
     3361 attttgtgat ttttgatcct tccttggcta caaattccac aacttcagca tcggcagaag
     3421 tgagacttct gacttcgagc acatcggcat ccaatgttaa ttgataaatg tagcttgccg
     3481 caatcatttt tttattgata acggtgccta tgtccaaatc ttcggccagt tggatgtaat
     3541 caatattctc aacttcggcc accgttttct ttactccaag tcgttttgct tccagacagg
     3601 ccagaatatt ggcttccgaa ttggcagtgg tggctacgaa tgcatctatc tcctgaatac
     3661 cttcggatcg cagagcgtcc acatttctgc catccgcatt gataatgagc gtattattga
     3721 gcctttcagc cagaaaataa catttttcct tgtctcgttc cagcattttg gtattgatat
     3781 tattgggcaa atattgtatc gttttttgag ctatacggct gccccccatt atcattgcat
     3841 ttttcacctg aaacaatgct ttcccggctt gttcgcgcac aaaatccacg ttttgcttca
     3901 ttgtgataaa ataaacaata tcaccggcct gaatttcgtc atatccgctt ggaatgatgg
     3961 tcttttcttt acgattgatt gctaccacgc ggtatttgcc atgatcaaag taaccagaat
     4021 taaagggttt tcttaaaacc tctgaatttt ccctgatttt cattcccagc aaaatgagtg
     4081 catcattgca gaaactgagg ttctgacgca gccatggatg tttgagagct tccacaattt
     4141 cttcggcagc cagtttttcc gggcaaataa gataatccac tcccagtttc tcaaaaaaat
     4201 ctttatttcc gggctgaaga tattcgtaat tttcaatgcg tgccaaagtt tttttggctc
     4261 caaggttagt agccagcata caagccgtca tattcacgct ttcaaagggt gtaacagcta
     4321 taaaaaggtc tgttccttcg atgccacatt ccttcagact ggcaatggag gtgggcaggc
     4381 ccacccgggt gagtaaatca taattggctt caagattgga cattttttcc ggatcttcat
     4441 ccatcaacgt gatgtccagg ctctcacggg caagtaattt agccaggtga gttcctactt
     4501 caccggcacc ggcaatgatt attttcattc agttactttt tttttataat ttcttttttt
     4561 tctaattctt ctatgagaac tgcctcaaag cttaccctta atccttcggc atgtaagccc
     4621 agtttggcat agagttcttc gggagttccg tgttcaacaa agctatcggg tattcccagc
     4681 cgggtaatgt tcatctgata attgttatca gccataaatt caagtacagc actgccaaat
     4741 ccgccatcga ttactccatc ttctacggta ataatatatt tgaatttttt tccaatacta
     4801 tgcagcaatt cttggtcgag cggtttaaca aagcgggcgt cataatgtgc tattgtcaca
     4861 tcccgttcat tttcaattat attaatcgct ttttcggccg gtgtagccat agtgccgatt
     4921 gtaatgatag ccagatcatt tccctctttc agacattcgc ctttgccaat ggtcaattcc
     4981 ttccatggct gatgccagtc aatgattgtt cctttaccac gtggataacg gattacaaac
     5041 ggacctttgc cgggcagttg agctgtgtac atgaggtggc ggagttcggt ttcattgcgc
     5101 ggagcagcaa tggtgaggtt ggggatgcac cgcatatatg ccagatcaaa ttctccctga
     5161 tgagtagccc cgtcggagcc aacaataccg gcacgatcca gacacataat aaccggcaaa
     5221 ttttgcagtg ccacatcatg aatgacatta tcatacgccc gctgcataaa tgaggaatag
     5281 atattgcaaa aaggcaccat accttctttg gccaaaccgg ctgaaaatgt cacggcatga
     5341 ccttcagcaa tacctacatc gaaaacccga tcgggaaacc ttttttgcat gataatcatg
     5401 gaacatcctg aaggcattgc gggtgtgaca cctataatat tcttgttttc tttggctaat
     5461 tccagcagtg tttttccaaa aacatcctga aacaagggag gttccacatt gccgtgatta
     5521 catacgagcc gttcacccgt ttccacatca aattttccgg gagcatgcca caccgttgcc
     5581 gatttctcag caggttcgta tcctctgccc tttttggtta ttagatggag tactttcgga
     5641 cctttgtaat tttttatttc ggtcaatatc ctgatcagat tatccacatc gtgcccgtca
     5701 accggaccaa aatagcggat attcattgct tcgaatatgt tctgtttctg acgtgtaaag
     5761 agcgtcttca gactgttatt tacccgtatg atttgttttt ttcgctcttc atccatccag
     5821 tgtctctttt taaagaaact gtaggctttg tacctcatct tattatacag acgagaggtg
     5881 gtaatatcta ccagatactg ggtaaaacct cctgaaattg ggtcaatagc catctgatta
     5941 tcattcagga ttatcagcag gtcattttta tccatcgacg tattgttgag cccttcgaat
     6001 gccaaaccac ccgtcatggc accatcgcct atcactgcca ccactttacg gtcctcaccc
     6061 ttcatctgag ctgacactgc catacccaat gcggccgaaa tggaggtaga ggcatgaccg
     6121 acaccaaaag catcgtactc actttcttct attttaggga aaccactgat acctcctaat
     6181 ttacggttgg tatgaaaaac atcgcgacgt ccggtcaata ttttatgccc gtaagcctga
     6241 tgacccacat cccacactat ccggtcataa ggtgtattaa agacataatg aagcgccacc
     6301 gtcagttcca ccacacccaa actggatccc aaatgtccgg gattttgcga aacttcatca
     6361 ataataaaat ctctaagttc ctgacaaaca acacccaatt cactgcgctt aattgtttta
     6421 aggtcattgg atgagtttat attattcagg tatttatatt ttcgttctat catattctat
     6481 aaaaaatcaa atacaaaaat aatacatttt tagcgggtgc gtgtattttt taataagatt
     6541 atttgaccgg gtaataaaat tacatttaat caaatcacaa tccttaataa aaataaccat
     6601 aaagcaataa ttttcatcca tacgacagat gtttacttac aatccaacaa ttttttctgt
     6661 ttttttcttc caaactattg ttgtttgtaa aaaacatatt atctttgcac acataaaaaa
     6721 aacaaatgat agctcaaagc atttacaacc tactagtcat tacacttttg gtcaaaaatc
//
LOCUS       DAOPZK010000105         6764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_13742_length_6764_cov_25.792368, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000105 DAOPZK010000000
VERSION     DAOPZK010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13742_length_6764_cov_25.792368"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1191)
                     /locus_tag="PKH68_06935"
     CDS             complement(28..1191)
                     /locus_tag="PKH68_06935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX12710.1"
                     /translation="MNIGEIRFTIVELSWLLVLLVAFGFQLYYYLRYFAGILRHRRRI
                     RKGKVEFLNQQPGVSVVICARDEEENLRKFLPLILEQDYPEYEVIVVNDASMDYTDDY
                     LSLMTKQYPNLHTTFVPAGATNMSTKKLGLTLGIKAARYDLLLFTDADCMPEGRNWIA
                     SMARNFTPATEFVLGYGGYLKKKGLLNILIKYDTLFIALQYMGMAIARRPYMGVGRNL
                     AYRSATFFRMKGFASTLHLQSGDDDLMINRGATSENTAVEISPESVTWSEPKKTFRQW
                     FIQKERHLSVSDQYNAASKLRLTTEPVSRALFYLSLIFVGLFGNWISIAAAGFIFLVR
                     YLIQAIIINKSAKQLGEQRFYLSILLLDIFLPLITLNIMVFGRKRKKKGIKWK"
     gene            complement(1259..2416)
                     /gene="lysA"
                     /locus_tag="PKH68_06940"
     CDS             complement(1259..2416)
                     /gene="lysA"
                     /locus_tag="PKH68_06940"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNX12711.1"
                     /translation="MLKAQYPIDKFKNIPTPFYYYDLNVLRDTLKEINQLTAGHNFEV
                     HYALKANVNPEILEIIKEAGLGADCVSGGEVQAAVNAGIPSSKIVFAGVGKADWEINL
                     ALENDIFCFNVESVPELEVINELAAAKNKTASVALRINPNVDAHTHNYITTGLNENKF
                     GINMEDIDKVINVLPDLKNIRMIGIHFHIGSQITDMTAYHNLCVRISEIEDTFASKNI
                     IVQHINVGGGLGINYEHPNHFPIPEFETYFRTFKEHFNLLPGQTLHFELGRSVVAQCG
                     SLISKVLYVKEGSTKNFVILDAGFTDLIRPALYQAYHRIENLSSDLPVEQYDVVGPIC
                     ESSDVFDKDCYLNKTRRGDIIAIRSAGAYGEIMASQYNLRALPKAYTSDKI"
     gene            complement(2484..3797)
                     /locus_tag="PKH68_06945"
     CDS             complement(2484..3797)
                     /locus_tag="PKH68_06945"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNX12712.1"
                     /translation="MKVLKFGGTSVGSAARMKDVAKLICDGEQKIVVLSAMSGTTNNL
                     VEIADYFYKGNTSGALEKINILENKYYDVAQELYSSSAKVSEADKIILSHFDFLRSFS
                     KSVFTVFEEKAILAQGELLSTAMFQLYLEETGVNSALIPALDYMRIDKNAEPDVAFIA
                     ENLKKILSETPDKEIYVTQGYICRNFYGEIDNLQRGGSDYTASLVGAAVKAQEIQIWT
                     DIDGMHNNDPRIVPDTKPVPYLHFEEAAELAYFGAKILHPTCILPAKLNNIPVRLLNT
                     MDPEAPGTLISNKSRKGVIEAVAAKDGITAIKIKSGRMLLAHGFLRKVFEIFESNQTS
                     IDMITTSEVGVSVTIDNTKRLDEILNDLKKFGTVTVDSDMVIICVVGDLPYETVGFQA
                     KVVDALKDIPIRMISYGGSNFNISILVKSEDKKRALNQLSQALFY"
     gene            complement(3993..4068)
                     /locus_tag="PKH68_06950"
     tRNA            complement(3993..4068)
                     /locus_tag="PKH68_06950"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4031..4033),aa:His,seq:gtg)
     gene            4155..4838
                     /locus_tag="PKH68_06955"
     CDS             4155..4838
                     /locus_tag="PKH68_06955"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNX12713.1"
                     /translation="MKYFIQNPQLEAIIKEIRKKIRLSMNGVVADKMKESGIRYKQNF
                     GVDIPRLKQIAAEYSQNHDLAQRLWTLQIRETMILATLLQPVDKFTPEMAEEWLSKLL
                     QPEIVEQTSMNLLAKLPYAVSLSLRWVNSTELWHQITGFILAARVWNDFDDSEREKII
                     HRAIELSDTAEYHLYKSIALALCRLARVSSRVAEQLLQRISFMKNAGISSHAYIYSEV
                     SREISFLDF"
     gene            4898..5362
                     /locus_tag="PKH68_06960"
     CDS             4898..5362
                     /locus_tag="PKH68_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNX12714.1"
                     /translation="MKEDKSFEAVKQGFTDFMVKNNLRKTRERYAILESIYKHTGHFN
                     AELLFNQMPKEYQVSLATVYNTLDLLQQCHLIVKHQFGEQGAQFEKTFGIAFHHHLVC
                     TECGKIREFPDLNINTLIQKKKFPRFETTRYSLYVYGLCNKCIAQQKKLGKH"
     gene            5432..6706
                     /locus_tag="PKH68_06965"
     CDS             5432..6706
                     /locus_tag="PKH68_06965"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNX12715.1"
                     /translation="MKVDVLLGLQWGDEGKGKVVDVLTPGYDVISRFQGGPNAGHTLE
                     FEGRKFVLRSIPSGIFQGDKINVIGNGVVLDPALFKDEVEALEKTGHPLTDRLRISKK
                     AHLILPTHRVLDMAYESAKGTGKIGTTGKGIGPTYTDKISRNGLRVGDILHNFEEKYK
                     AAIERHLEILKHYNVEYDLAALEKSWFEGIEKLKEFEFIDSEHFVNEAIRNGKKVLAE
                     GAQGTMLDIDFGSYPFVTSSNTICAGACTGLGVAPRNIGEVYGIFKAYCTRVGSGPFP
                     TELFDHDGDEMRRIGCEFGSVTGRPRRCGWIDLVALRYAIMINGVTQLIMMKGDVLDA
                     FDTIKACVAYRINGVETEEFPFELNDGVEPVFVELPGWKTDLTKIKSEDEFPEELNAY
                     LDFLEEELGVPVKIVSVGPDREQTIIRHEEDV"
BASE COUNT         1912 a         1449 c         1511 g         1892 t
ORIGIN      
        1 tgaaaaatga tctttataac aaatacctta tttccactta attccttttt tcttcctctt
       61 cctgccaaat accataatat tcagtgtgat gagcggcaga aaaatgtcga gcagcagaat
      121 agacaggtag aaacgctgct ctccaagttg tttagccgat ttgttgatga taatggcctg
      181 tatcaggtag cgcaccagaa aaataaatcc ggcggcagca atgcttatcc agttgccgaa
      241 aagtcctacg aagatcagag agagataaaa tagtgcccgg ctgacaggtt cggtggtcaa
      301 ccggagtttg cttgcagcgt tgtattgatc agaaaccgac agatggcgtt ctttctggat
      361 aaaccattgt ctgaatgtct tttttggttc tgaccaggtc acactttcgg gcgaaatctc
      421 tacggctgta ttttcgctgg tagcaccacg gtttatcatc aggtcatcgt cgcccgactg
      481 cagatgaagg gttgatgcaa agcctttcat tctgaaaaat gtggcgctcc ggtaagccag
      541 attgcgtccc acacccatgt atggtctccg ggcaatggcc atgcccatgt attgcagggc
      601 gataaacagc gtgtcgtatt tgataagtat gttcagcagc ccttttttct tcaggtagcc
      661 gccgtaaccc agcacaaatt ctgtggcagg tgtgaaattt ctagccatgg aagctatcca
      721 gtttctccct tcaggcatgc aatcggcatc ggtgaacagg agcaaatcgt atcgggctgc
      781 ttttattccg agtgtaagcc ccagtttctt ggtactcatg ttggtggcgc cggccggtac
      841 aaaggtggtg tgcaggtttg gatattgttt ggtcatcagt gacaggtaat cgtcggtgta
      901 gtccatcgaa gcatcattca ccacaatgac ttcatattcg ggataatcct gttcgagtat
      961 caggggcaga aatttacgga gattttcctc ttcgtcgcgg gcacaaatta cgacagatac
     1021 gcccggctgc tggttcagaa actcaacttt gccttttcgg atgcgacgac ggtggcgcaa
     1081 aattccggca aaatacctca ggtaatagta cagctgaaat ccgaatgcga ccagcaatac
     1141 aagcaaccag ctcaattcaa cgatagtaaa tcgtatttcc ccaatattca tattcaatct
     1201 taaaaaaaaa aagaaaccgg tgtaataatt ctaatcaata aaaaaggtaa acaaattatc
     1261 agattttatc cgatgtatag gcttttggca atgcccgcag gttgtactgc gaagccataa
     1321 tttcaccgta agcacctgcc gaacgaatag caataatatc gccccgtcgg gttttattga
     1381 ggtaacagtc cttatcgaat acatccgacg attcgcagat gggacctacc acatcatact
     1441 gctccaccgg taaatccgaa ctcagatttt caatccggtg ataagcctga tagagggcgg
     1501 gacggatcaa atcggtaaat ccggcatcga ggattacaaa attcttagtt gaaccttcct
     1561 ttacgtacaa taccttcgaa atgaggcttc cgcattgagc caccaccgat cgtcccagtt
     1621 caaagtgaag tgtctgaccc ggaaggaggt tgaaatgctc cttaaaagtc cggaaatacg
     1681 tttcgaattc cggtatcggg aagtggttgg ggtgctcgta gttaatgccc aggccaccgc
     1741 caacattaat atgctgaacg ataatatttt tcgaagcaaa agtatcttca atttcggaaa
     1801 tacgcacaca cagattgtgg taagccgtca tatcggtaat ctgcgagccg atgtgaaaat
     1861 gtatgccaat catacggata tttttcaggt cgggcaacac gttgataacc ttatcaatat
     1921 cttccatatt gataccgaat ttattctcgt tcagtccggt ggtgatgtag ttgtgcgtgt
     1981 gagcatccac atttggattg atgcgcagtg ctaccgatgc tgttttattt tttgcagcag
     2041 ccagttcgtt gatcacttcc agctcgggca cagattctac attgaaacag aaaatgtcat
     2101 tttcaagtgc cagatttatt tcccagtcgg ctttacccac tcctgcaaaa actattttgg
     2161 acgatggaat accggcatta acagcagcct gaacttctcc gccactcacg cagtcggcgc
     2221 ccagcccggc ttctttgata atttcaagaa tttcgggatt aacattggct ttgagggcgt
     2281 aatgcacttc aaagttatga cctgcggtaa gttggtttat ttctttcaat gtatcgcgaa
     2341 gcacatttaa gtcgtaataa taaaagggcg tcgggatatt tttgaattta tcgatggggt
     2401 attgtgcctt taacataatc tgctgtaaat ttatttctta aaaaaccgat aagttttatt
     2461 ggcttaccgg tttttttatt tgtttaatag aataatgcct gacttaactg gttgagtgct
     2521 cgtttcttgt cttccgattt taccagaatg gaaatgttga agttgcttcc accataggat
     2581 atcatccgga tgggtatgtc tttaagtgca tctactactt ttgcctggaa gccaaccgtt
     2641 tcgtaaggca gatcgcccac cacacagatg attaccatat cactgtcaac ggtaacggta
     2701 ccaaattttt tcagatcgtt caggatttca tccagtcgtt tggtgttgtc gatagtcacc
     2761 gatacaccca cttcggaagt ggtaatcata tcgatggaag tctgattgct ttcaaagatt
     2821 tcgaacactt tgcgcaggaa accgtgtgcc agcaacatac gtcccgattt gatcttgata
     2881 gcagtgatgc cgtccttggc tgccacagct tcaatcactc ctttgcgcga tttgttggat
     2941 atcagtgtac ccggtgcttc agggtccatg gtattgagca accttaccgg gatgttattg
     3001 agtttggccg ggagaataca ggtgggatgc aaaatttttg caccgaaata ggccagctcg
     3061 gcagcttcct cgaagtgcag gtagggaacg ggttttgtgt ccggtactat gcgcggatcg
     3121 ttgttgtgca ttccgtcaat atcggtccat atctgaattt cctgcgcttt gacagccgca
     3181 cctaccagcg aagcagtata atcgcttccg ccgcgctgaa ggttgtcaat ttctccgtag
     3241 aagttacggc agatatatcc ctgtgtgaca taaatttctt tgtcgggagt ttcggaaaga
     3301 atttttttca gattttcggc gataaatgcc acatcgggtt ctgcattttt gtcaatgcgc
     3361 atatagtcca gtgccggaat gagtgcgctg ttcacaccgg tttcttccag gtaaagctgg
     3421 aacatggctg tcgacagcaa ttcgccctga gccagtatgg ctttttcttc aaatacggta
     3481 aatacgcttt ttgagaaaga acgcagaaaa tcgaagtgac taagaataat tttatccgct
     3541 tccgaaactt tggctgatga actgtagagt tcctgagcta catcgtaata tttattttcc
     3601 agaatgttga ttttttctaa cgctccggag gtattccctt tataaaaata atcggcaatt
     3661 tctaccagat tatttgttgt gccggacatg gccgacagca caacaatctt ttgttcaccg
     3721 tcacaaatta gcttagctac atccttcatg cgggctgccg aacctacaga agttccaccg
     3781 aatttaagta ctttcatttt ttctgattta taaatgttta tcctaatgaa aattgagtgc
     3841 aaaaataaca ctttttttat aaataagagt gatttggttt tcccgttatt tgagttaatt
     3901 ttgcaaaacg ataatgataa tgccgtatta gttgacaaaa tgcctttttt gcaattaaaa
     3961 aaaggaaacc cattgctgaa tttccttttt cggggtggaa aacggggctc gaacccgcga
     4021 ccttcggaac cacaatccga cgctctaacc aactgagcta caaccaccat gtttttgcga
     4081 gtgcaaagat agtatagaat cggatacttt gcaaattatt ttgaaagaaa aaatgtaaac
     4141 ataatattaa atccatgaag tattttatcc aaaatcctca gttagaggca atcattaaag
     4201 aaattcgaaa aaaaataaga ctatcgatga acggtgttgt ggctgataaa atgaaggaaa
     4261 gcggtatccg ttacaagcaa aatttcgggg tggacatccc gcgtctgaag cagattgccg
     4321 ctgaatattc ccaaaatcat gatttggcac aacgactctg gacactccaa atccgtgaga
     4381 caatgatact ggccacactg ctccaaccgg ttgataaatt tacccccgaa atggctgaag
     4441 aatggctttc aaagctcctt cagcccgaaa tagtggagca aaccagcatg aatctgctgg
     4501 caaaactgcc ctatgcagta agtctttcgc ttcgttgggt aaattccact gagctgtggc
     4561 atcagataac gggtttcatc ctcgctgccc gtgtgtggaa tgattttgat gacagtgaaa
     4621 gggaaaaaat aattcatcgt gctatagaat tatccgatac tgcagaatat catctttata
     4681 aatccattgc actggcactg tgccggctgg cacgggtgag cagcagggtg gcagagcaac
     4741 tgctgcaacg catcagtttt atgaaaaatg ccggtatttc ttcgcatgcc tacatttaca
     4801 gcgaagtaag ccgcgagata tcttttttgg atttttaata gataatctca tcaaaatatc
     4861 gtacttttgt tctgatttta accaaataat ccgttaaatg aaagaagata aatcatttga
     4921 agcagtaaag cagggtttta ccgattttat ggtaaaaaat aaccttcgca aaacgcgcga
     4981 aagatatgcc atcctggaaa gcatttacaa acacacgggg catttcaacg ctgagctgct
     5041 cttcaaccag atgcctaagg aatatcaggt aagtctggcc acggtttaca ataccctgga
     5101 tcttctccaa caatgtcatt taatagtgaa acaccagttt ggggaacagg gagcgcaatt
     5161 tgaaaaaact ttcggcatag cctttcatca tcacctggtg tgtaccgaat gtggtaaaat
     5221 cagggaattt ccggatttaa atatcaatac attaatacaa aaaaagaaat ttccccggtt
     5281 cgaaaccacc cgttactccc tgtatgtgta tggattgtgt aataagtgca ttgcgcagca
     5341 aaaaaagctg gggaaacatt aatcagaatc aaaaaaccga caacttatgg aggggcacga
     5401 gcctcacgat aagctaataa ctgaccaaca aatgaaagta gatgtgctat tgggccttca
     5461 gtggggcgac gaaggcaaag gtaaagtagt ggacgtactc acacccgggt acgatgtgat
     5521 aagccgtttt cagggtggac ccaatgcggg tcatacactc gaattcgaag gacgaaaatt
     5581 cgtcttacgc tccattcctt ccggaatttt tcagggagat aaaatcaatg taattggcaa
     5641 cggtgtggtg ctcgatcccg ctctttttaa agatgaagtg gaagctcttg agaaaacggg
     5701 acatccactt accgaccgtc tccggatttc gaaaaaagca catcttattc tgcctacaca
     5761 ccgggtgctc gatatggctt acgaatcggc taagggaacg ggcaaaattg gtactaccgg
     5821 taaaggtata ggacccactt acaccgacaa aattagccgt aacgggctga gagtagggga
     5881 tatacttcat aattttgaag aaaaatacaa ggctgctatc gaaaggcacc tggaaatact
     5941 gaaacattac aatgttgaat acgatctggc agcactcgaa aaaagctggt tcgaaggaat
     6001 tgaaaaacta aaggaatttg aatttatcga cagcgaacat tttgtaaacg aggccattcg
     6061 gaacggtaaa aaagtactgg ctgaaggtgc tcagggaacc atgctcgaca tcgatttcgg
     6121 ttcttatccg tttgtaacct catccaatac catctgtgcc ggcgcctgta ccggacttgg
     6181 cgtggctcca cgcaatatcg gcgaggtgta cggaatcttc aaagcttatt gtacccgcgt
     6241 tggcagcgga ccgtttccca ccgaactttt tgatcacgac ggtgatgaga tgcgccgcat
     6301 tggatgtgag ttcggttcgg tcacgggtcg cccacgccgt tgcggttgga ttgatctggt
     6361 agcgctccgt tatgccatca tgatcaatgg agtaacccaa cttatcatga tgaaaggcga
     6421 tgtgctcgat gctttcgata ccattaaagc ctgcgtggct tatcgcatca acggtgtgga
     6481 aaccgaagaa ttccccttcg aactcaacga cggtgtcgaa cctgtatttg tggaattgcc
     6541 cggatggaaa acagacctca cgaaaataaa aagcgaagat gaatttcccg aagaactgaa
     6601 tgcttacctt gattttctgg aagaagaact cggcgtacct gtgaaaattg tttccgtcgg
     6661 tcccgaccgc gaacaaacca ttatccggca cgaagaagat gtttaatgtt tagtgtttag
     6721 tgtttagtgt ttaatgttaa gtgtctaatt gtttaatcgt tgaa
//
LOCUS       DAOPZK010000106         6532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_14430_length_6532_cov_21.572178, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000106 DAOPZK010000000
VERSION     DAOPZK010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14430_length_6532_cov_21.572178"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1103
                     /locus_tag="PKH68_06970"
     CDS             <1..>1103
                     /locus_tag="PKH68_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX12716.1"
                     /translation="AVYSKKDNSAAPITRIEPILNYENLSLIKNFPQHFDLNNSKVSW
                     NGQLEAAESGEFHFILHYAGYVKVYLNNEPVVKERWRTAWNPNDFKFKYNFKVGEKVP
                     VRIEWEPDGGVSYISLKALSPIPENEQNKLALWSEMADEIDYYFVYGRNMDEVVSGYR
                     TLTGKAQVMPKWAMGFWQSRERYKTQDELLGTLSEFRKRNIPIDNIVLDWFYWPEKEW
                     GSHKFDPLRFPEPKAMVDSVHKMNGHIMISVWPKFYITTDNYKAFDKNGWMYQQAVKD
                     SIRDWVGKGYVGSFYDAYSADARKLFWKQMQESLYTKGFDAWWMDASEPNVQDNTDMD
                     YRKKLCGPTALGPSTKYFNAYALMNAEAIYDGQR"
     assembly_gap    1104..1203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1204..2324
                     /locus_tag="PKH68_06975"
     CDS             <1204..2324
                     /locus_tag="PKH68_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX12717.1"
                     /translation="YFNAYALMNAEAIYDGQRGVEPNKRVFLLTRSGYAGLQRYSTAT
                     WSGDIGTRWEDMKAQISAGLNFAISGIPYWTMDIGGFCVEKRYENAQRLFDTTGTENN
                     DLKEWRELNTRWYQFGAFVPLYRAHGQFPLREIFNIAPENHPAYQSILYYSKLHYRLM
                     PYIYSLAGMTHFNDYTIMRALVMDFGKDTVVNNISDQYMFGNALMVCPVYEYKAVKRT
                     VYFPAKTGWYDFYTGKHIAGGNRMAVDAPYSRLPLYVREGAIIPFGPEIQYASEKKPE
                     LITLYVYGGQNGSFTLYEDEGENYNYEKGSYSTIQFNYNEASKSLEIADRKGSYDGML
                     VNRKFNVVYVDKSTPKAADPDAKGIVINYNGTKQLIKF"
     gene            2328..4829
                     /locus_tag="PKH68_06980"
     CDS             2328..4829
                     /locus_tag="PKH68_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX12718.1"
                     /translation="MKRIISGLTVLIFFTLNVSAGQNPRTVSNFNSGWNFRLGDVAKA
                     FSPRFNDADWRKLNLPHDWAVEGDFSENNPAGSGGGALPGGIGWYRKTFKADKKLIGK
                     KIFIDFDGVYSNSEVWLNGVSLGVRPYGYISFRYELTKYLKFDADNVIAVRVDNSEQP
                     NSRWYSGCGIYRNVWLTITDPVHVDLWGTYVTTPEVSREKATVNVVTTVKNDNNSPAD
                     VKIITIVENASGKKITEVQSVLKLETGKTNISSQQLNLNNPVRWTLENPYLYRVVTRL
                     FVENKLKDEYTTPLGIRTFSFDADKGFFLNGVPTKIKGVCMHHDLGCLGAAVNTRAIE
                     RQLQIMKEMGVNGIRTSHNPPASELLDLCDRMGFIVMDETFDMWRKKKTAYDYSRSFN
                     EWHERDLTDHILRDRNHPSVFMWSVGNEVLEQWTHADADTLDIQQANLLLNLKRDPKM
                     LAQDTEKMSVNSLLAKKLAEIVKKLDPTRPVTSANNEPSPDNHLFRSGAMDIIGFNYH
                     ETYFDGVKKNFPGKPFIVTESTSALMTRGYYRMPSDTMFIWPERWDLPFSEPSYSCSS
                     YDNCHVPWGTTHEKSWELVKNNDFISGMYIWTGFDYIGEPTPFWYPAKTSYFGIVDLA
                     GIPKDVYYMYQSEWTDKDVLHVFPHWNWTKGQTVDVWAYYNHADEVELFLNGISQGVK
                     KKADNVFHVSWRLSYQPGTIKVVSRLNGREVLTKEIKTAGKPAQIKLTPDRTVLNADG
                     TDLSFVTVEILDKDGNLCPNADNPVKFNVEGNGFVAGVDNGNQISLERFKASERKAFY
                     GKCLVVIQNSGKKGDINLKAVSDGLNADIVKIKVR"
     gene            4858..6294
                     /locus_tag="PKH68_06985"
     CDS             4858..6294
                     /locus_tag="PKH68_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNX12719.1"
                     /translation="MKILENHFRQFVLILLIAFFTDCKTETPVVPEITLDKQEMQFTY
                     ASGTNQLAITANLPWTASVSQSWCALSSSSGDAGTKQLTVSVTENTSDAIREAIITFT
                     SGSVSKQVKVVQAKSNDVPGMMSDALVLAKKMTLGWNLGNALEASGGETAWGNPKTTK
                     ALIDAVKAAGFNAVRIPCAWNSYLEDQTTYKIKVSWLARVKEVVDYCVDNNMYAILNI
                     HWDGGWLEENPTYAKQTAVNAQQKALWEQIANYFREYDEHLLFAGTNEVHAGYGTPTT
                     ENITVQLSYNQTFVNAVRSTGGNNACRNLIVQAYNTNIDLAVSSLKMPTDNVANRMMA
                     EVHYYDPWDFCGDAAGTIYFWGADFKKYGSVSTWGQESYVDQQFAKMKTNFVNKGIPV
                     ILGEYGAIRRLTLTGDALTHHLDSRAYYLKYVTQQAKNNGMVPFYWDNGGPGDKSFGI
                     FNRNTNSAGDPKALSGLVQGAAAGVYPY"
BASE COUNT         1939 a         1360 c         1488 g         1645 t
ORIGIN      
        1 gctgtttatt ccaaaaaaga caattcggcg gcgcccatca cacgcattga acccatcctg
       61 aattatgaaa atctttcgct gataaaaaat tttcctcaac attttgatct taacaactcc
      121 aaagtaagct ggaatggtca gctggaagct gctgaaagcg gggaatttca ttttattcta
      181 cactatgccg gatatgtaaa agtatatttg aataatgaac cggtagtgaa agaacgctgg
      241 cgtactgcct ggaacccgaa cgatttcaaa tttaaataca atttcaaagt cggtgaaaaa
      301 gttcctgtcc gcatagagtg ggagcccgac ggaggtgttt catacatttc actgaaagca
      361 ttgagtccga taccagaaaa tgaacaaaac aaactggctt tgtggagcga aatggccgac
      421 gaaattgact attatttcgt ctatggccgc aatatggatg aagtagtcag cggataccgg
      481 acacttaccg gcaaggcgca ggtaatgccc aaatgggcga tgggtttctg gcagagccgc
      541 gaacgctata aaacacagga tgaactgctc ggcacgttat ccgaattccg caaacgcaat
      601 attccaattg ataatattgt tctcgactgg ttttactggc ccgagaaaga gtggggaagt
      661 cataaattcg atccgctgcg tttcccggaa cccaaagcaa tggtggacag cgtacacaag
      721 atgaacggac acatcatgat ttccgtttgg ccaaaattct atattactac cgacaattac
      781 aaagcatttg acaaaaacgg ctggatgtat cagcaggctg tgaaagacag cattcgcgac
      841 tgggtgggaa aaggctatgt gggttcattt tacgatgctt attcggcgga tgcccgtaag
      901 cttttctgga aacaaatgca ggaaagtctt tacacgaaag gatttgatgc ctggtggatg
      961 gacgcttccg agccgaatgt acaggataat accgatatgg attaccgcaa aaaactctgc
     1021 ggaccaactg cactcggacc ttccaccaag tatttcaatg cctatgcgct gatgaatgcc
     1081 gaagctattt atgacggtca gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnagtattt caatgcctat gcgctgatga atgccgaagc tatttatgac ggtcagcgcg
     1261 gagtggagcc caacaaaaga gtgtttttac tgacccgttc gggttatgca ggcttgcaac
     1321 gatattccac tgccacatgg agcggcgata ttggtacacg ctgggaagat atgaaagcgc
     1381 agatttcggc cggactgaac tttgcaattt ccggaattcc atactggacg atggatatcg
     1441 gcggtttttg tgtagagaaa cggtatgaaa atgcgcaacg attattcgat actaccggaa
     1501 cagaaaacaa tgacctcaaa gaatggcgtg aactgaatac ccgctggtat cagtttggag
     1561 catttgtgcc gctgtaccgt gctcacggac aatttccgct tcgcgaaata ttcaacattg
     1621 cccctgaaaa tcatccggct tatcagtcca ttttgtatta cagcaaactg cattaccggc
     1681 tgatgccata catctactcg ctcgcaggca tgacccattt caacgattac accattatgc
     1741 gtgctctggt gatggatttc gggaaagata ccgtagtcaa taatatcagc gaccagtata
     1801 tgtttggaaa tgcactgatg gtttgtccgg tgtatgaata caaagcggtg aaacgtactg
     1861 tttatttccc tgccaaaacc ggatggtatg atttctacac cggaaaacac attgccggag
     1921 gaaaccggat ggcagtcgat gcaccatatt ctcgccttcc gctgtacgtt cgcgaaggag
     1981 caatcattcc tttcggacct gaaattcagt acgcttccga gaagaaaccg gaattaatta
     2041 ccctgtatgt gtatggcgga caaaacggtt cctttacgct gtatgaagat gaaggtgaga
     2101 actataacta cgaaaaaggt tcgtattcca ccattcagtt taattacaac gaagcttcaa
     2161 aatcccttga aatagccgac cggaaaggat cgtatgacgg aatgcttgta aaccgcaaat
     2221 ttaatgtggt ttatgtggat aaatcaactc cgaaagctgc cgatccggac gctaagggaa
     2281 tagtaatcaa ttacaacgga actaaacagc tcataaaatt ttaagttatg aaacgaataa
     2341 tctcaggttt aacagttctg atatttttta cactgaatgt gtctgccggt cagaacccgc
     2401 ggactgtgag caactttaac tcaggttgga atttccggtt gggtgatgtt gcaaaagcat
     2461 tttctcccag atttaacgat gcagattggc ggaaacttaa tctcccacac gattgggcgg
     2521 ttgaaggtga tttcagtgag aataatcctg ccggaagtgg aggaggagca ttgcccggag
     2581 gcattggctg gtaccggaaa acattcaaag ccgataaaaa actcataggt aagaaaatat
     2641 tcatcgattt tgatggagtt tacagcaatt cggaggtctg gctgaatggc gttagcctgg
     2701 gagttcgtcc atacggctat atttcattcc ggtatgagtt gacgaagtat ttaaaatttg
     2761 atgctgataa cgtgattgct gttcgggtgg ataacagcga acaacccaac tcccgctggt
     2821 attcgggctg tggaatatac cgcaatgtct ggctcacaat caccgatcct gtacacgtgg
     2881 atttgtgggg aacgtatgtc accacacccg aagtaagtcg tgaaaaagca acggtaaatg
     2941 tcgtaacaac agtcaaaaac gataataaca gcccggcgga tgtaaaaatc atcacaatcg
     3001 tcgaaaatgc ttctggaaag aaaatcacag aggttcaatc cgtgctgaaa cttgaaaccg
     3061 gaaagaccaa catatccagc caacaactga atttgaacaa tccggtacgt tggacacttg
     3121 aaaatccgta tctgtaccgc gtggttaccc ggctttttgt ggaaaataaa ctgaaagatg
     3181 aatacaccac tccgctgggt atcagaactt tctcctttga tgccgataaa ggtttcttcc
     3241 tgaacggagt accgaccaag attaaaggtg tatgcatgca tcacgatttg ggttgcctgg
     3301 gagctgctgt caatacaaga gctattgaac gccagttgca gataatgaaa gaaatgggtg
     3361 tgaatggcat ccgtacatcg cacaatccac ctgcatccga actgctcgac ttgtgcgacc
     3421 ggatgggctt cattgtgatg gatgaaacct ttgatatgtg gcgaaagaaa aaaacagcct
     3481 atgactattc ccgctcattc aacgagtggc atgagcgcga cctcaccgac catatcctgc
     3541 gcgaccgcaa tcatccgtcg gtatttatgt ggagtgtggg caacgaggtg ctcgaacagt
     3601 ggacacacgc cgatgccgat acgctggata ttcaacaggc aaatttactc ctcaatctga
     3661 aacgtgaccc gaaaatgctt gctcaggata ctgaaaagat gagtgtaaac tcgctattgg
     3721 ccaaaaaact tgctgaaata gtgaaaaaac tggatccaac ccgccctgta acatcggcta
     3781 ataatgaacc cagccccgat aatcatcttt tcagatcagg agccatggat attattggtt
     3841 ttaattatca cgaaacgtat tttgacggtg taaaaaagaa ttttcccgga aaaccattca
     3901 tcgtgaccga atcaacttcc gctctgatga cacgcggata ttaccgcatg ccttccgaca
     3961 ctatgttcat ctggccggag cgctgggatc tgcctttttc agaaccgagc tattcctgct
     4021 cctcgtatga taactgccat gtgccgtggg gaaccaccca cgaaaaatcg tgggaactgg
     4081 tgaaaaacaa tgattttatc agtggcatgt atatctggac cggatttgat tacattggtg
     4141 aacctacacc gttctggtat cctgccaaaa cttcctattt cggaattgtg gatctggccg
     4201 gtatacccaa agatgtttac tacatgtacc aatccgaatg gaccgataag gatgtattgc
     4261 atgttttccc gcactggaac tggacgaagg gacaaaccgt ggatgtctgg gcatattaca
     4321 atcacgcgga cgaagtggag ttattcctca acgggatttc gcagggcgta aagaaaaagg
     4381 ctgataatgt attccatgtg agttggcgac tgagctatca gccaggaacc atcaaagtag
     4441 tttcccgcct taacggacgt gaagtattga ccaaagaaat taaaactgcc ggtaaaccag
     4501 ctcagataaa actcacaccc gaccgaaccg tgttgaatgc tgatggaact gatttgagtt
     4561 ttgtaacggt agaaatactc gataaggatg gcaatctgtg cccgaatgct gataatccgg
     4621 tgaaattcaa cgtagaaggc aacggctttg tggccggagt agataatgga aatcagatca
     4681 gtctggaaag atttaaagct tcggaacgaa aagcattcta tggaaaatgt ctggtagtca
     4741 ttcagaattc aggcaaaaaa ggagatatta atctgaaagc agtctctgat ggactcaatg
     4801 ctgatattgt aaaaataaaa gtcagataaa ccggcaatta atcacctgta tttacgcatg
     4861 aaaatattag aaaatcattt cagacaattt gtgctaattt tacttattgc ttttttcacc
     4921 gattgcaaaa cagaaacacc ggttgttcct gaaataacgc tggataaaca ggaaatgcaa
     4981 tttacctatg caagcggaac caatcagctg gcaattacgg ctaatctacc gtggacagct
     5041 tccgtttctc aatcgtggtg tgcgttatct tcctcatcgg gcgatgccgg gacaaaacaa
     5101 cttaccgttt cggttactga aaacacttcc gatgcaatac gtgaagccat aatcaccttc
     5161 acttccggct cagtatcaaa acaagtgaaa gttgttcagg ctaaaagcaa cgatgtaccc
     5221 ggtatgatga gcgacgcact tgtgctggca aaaaaaatga cattgggctg gaatttgggc
     5281 aatgcacttg aagcatcggg aggcgaaaca gcttggggaa atcctaaaac cacaaaagca
     5341 ttgatagatg ccgtaaaagc cgccggattt aatgccgttc gtattccatg tgcatggaac
     5401 agctatctgg aagatcagac aacttataaa atcaaagttt cctggttggc acgggtaaaa
     5461 gaagtggttg attattgcgt ggataataat atgtatgcta tcctgaatat tcactgggat
     5521 ggcggctggc tcgaagaaaa tccgacttat gccaaacaaa cagctgtaaa tgcccaacaa
     5581 aaagcattgt gggaacagat agccaactat ttcagggagt acgacgaaca tttattgttt
     5641 gcaggtacca atgaggtgca tgccggatat ggcacaccaa ctaccgaaaa tattaccgta
     5701 caattatcct ataatcagac ttttgtgaat gcagtccgct ccaccggagg aaataatgcc
     5761 tgccgcaatc tcattgttca ggcttacaat accaatatcg acctggctgt ttccagtctg
     5821 aaaatgccca ccgacaatgt ggctaaccgc atgatggcag aagttcatta ttacgatccg
     5881 tgggatttct gcggcgatgc agccggtact atttattttt ggggagctga tttcaaaaag
     5941 tatggttccg tttccacctg gggacaggag agttatgtcg atcagcagtt tgccaaaatg
     6001 aaaacaaatt ttgtaaacaa gggaattcct gtgatactgg gtgaatacgg tgcaatcaga
     6061 agacttacat taacgggcga tgcattaact catcatctgg actcccgggc ttactacctg
     6121 aaatatgtaa cccaacaggc gaaaaataac ggaatggttc cattttattg ggataacgga
     6181 ggtcctggcg ataaaagctt tggcattttt aaccgcaata ccaattcggc aggagaccca
     6241 aaagcgctga gcggtctcgt gcagggagct gctgctggtg tatatccata ctgaagagta
     6301 atcagtgttt tataacatat taactagaca aagaaaatcc ccgggtaact atttcccggg
     6361 gatttgattt tttaattatt attctgattt acaggacggt ttttctccga atattccttg
     6421 ggcgacaggc catagtaatc cttaaatgaa ttggaaaaat gcgaaagatt ggaatatccc
     6481 agagcataag ccacttccga aatatttacc ttttgtttgg acagaatttc ag
//
LOCUS       DAOPZK010000107         6526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_14447_length_6526_cov_23.307989, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000107 DAOPZK010000000
VERSION     DAOPZK010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14447_length_6526_cov_23.307989"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1210)
                     /gene="serC"
                     /locus_tag="PKH68_06990"
     CDS             complement(131..1210)
                     /gene="serC"
                     /locus_tag="PKH68_06990"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNX12720.1"
                     /translation="MKKHNFNAGPCVLPKPAIEAAIDAIRDFDGTGIGILEISHRTPG
                     WERIMAETEQLWRDLLNIPDEYAVLFLGGGASTQFFYLAANLLNKKAAYLQTGVWAKK
                     AIKEAKFYGEVEVVASSEDKNYSYIPKGYTIPTDVDYFHITTNNTIYGTEIHEDITSP
                     VPLVADMSSDIMSRPVDVSKYALIYGGAQKNVGPAGVAFVIVRKDILGKADRKLQTMV
                     DYRTHIGETPKDNSMFNTPPVFSIFVMHETLKWVKDLGGVEAMHKLNMQKANLLYDEI
                     DRNKMFVGTAVKEDRSIMNVCFVMAEEYKDKEADFMAFAKERGMVGIKGHRSVGGFRA
                     SIYNACPLESVEALVACMQEFEKLN"
     gene            complement(1397..2797)
                     /locus_tag="PKH68_06995"
     CDS             complement(1397..2797)
                     /locus_tag="PKH68_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNX12721.1"
                     /translation="MKKTTFILLSFLISLSTFAQNITGSWNGILKVQGQQLRLVINIQ
                     QAENGYKATMDSPDQGAKGIPVDRVTFANDTLKFEVKMIGVTYTGVLGQDKVIKGTFT
                     QMGMSFSLDLSAQPVEKEKVLRPQEPQKPYPYYSEEVKFTNPNGDTLAGTLTLPKKEG
                     KFPVVVMITGSGPQNRDEELMGHKPFLVIADFLTRNGIGVLRYDDRGTAQSTGDFQNA
                     TSVDFANDTKAAVRYLSTRKEINKKKIGLMGHSEGGLIAPMVAVRSKKAAFIVMLAGP
                     GMRGKELLVLQTEAISKAYGMSEQEINKTIKLNSGAYDIVLNSNDTTKLKTDLKNYFL
                     NNIKDLPGLTENATQEQSDMQVKAYTDQLSSPWMQFFIKYDPVPMLKKVKCPTLALNG
                     SKDLQVPSAENLAAIRKAFSESGNKKLTAVELPGLNHLFQESTTGSPAEYAKIEQTFS
                     PKALEEILKFIRIQTK"
     gene            complement(2921..5536)
                     /gene="mutS"
                     /locus_tag="PKH68_07000"
     CDS             complement(2921..5536)
                     /gene="mutS"
                     /locus_tag="PKH68_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNX12722.1"
                     /translation="MTNDVVETPMMKQFFDIKRKYPDAVLLFRCGDFYETYSTDAVEC
                     AGILGITLTRRANGSAKTVEMAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLTKKI
                     VKRGVTELVTPGVSYSDTTLTQKENTFLASVYLNKNQAGVSFLDISTGEFLIAEGTVD
                     YVDKLLSSFSPKEVIYDRSKRSEFEANFGTKYFTFALEDWGFSPETANERLLKHFQTK
                     NLKGFGVENLKNGIVAAGAILHYLDMTHHHQTEHITHLTRIEEERFVWLDRFTVRNLE
                     LYGTINEGGRTLVDVIDRTISPMGGRLLKRWVAFPLKDVKPIEERLSVVEYFFRDTEL
                     KHMLEQQIALIGDLERIISKVAVGRINPREVVQLKVALQAIEPIKNACAEVENVTLRK
                     IADQLNPCKVISEKIEAELQNNPPVLVNKGDIIRSGVNTDLDELRRLAHSGKSYLLEI
                     QQRESATTGIPSLKISYNNVFGYFIEVRNMHKDKVPETWIRKQTLVNAERYITQELKE
                     YEEKILGAEEKILAIETRLFNELVISLTDYIAAIQLNSNLIAQLDCLLSFSKVSSENK
                     YVRPEINDSDVIDIRQGRHPVIEKQLPIGETYVANDVYLDGKTQQIIIVTGPNMSGKS
                     ALLRQTALITLMAQIGCFVPVESAKIGVVDKIFTRVGASDNISQGESTFMVEMNEAAS
                     ILNNLSDRSLVLFDELGRGTSTYDGISIAWAIVEYIHEHASCKAKTLFATHYHELNEM
                     ENSFSRIKNYNVSVKEVDKKVIFLRKLVRGGSEHSFGIHVAKMAGMPQSIVKRAEKIL
                     VQLEADNRQNGDLSKPVGELATQREGYQLSFFQLDDPVLEQIRDEIKGLDVNNLTPIE
                     ALNKLNEVKRIITGK"
     gene            complement(5554..6387)
                     /locus_tag="PKH68_07005"
     CDS             complement(5554..6387)
                     /locus_tag="PKH68_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNX12723.1"
                     /translation="MTKQNAIKLFEEKKVRTVWDDEAEEWYFSVVDVIEVLTESENPR
                     KYWSVLKTRLKKEGNQTATNCSQLKMDASDGRKRLTDVATTQQLFRLIQSIPSPKAEP
                     FKLWIAQVAKERLDQMQDPELSIEQAMMDYKRLGYSDNWINQRLKSIEIRKDLTDEWK
                     KRGLEEGIQFATLTDIIYKTWADKTAKEYKQFKGLKKENLRDNMTNKELVLNMLAELS
                     TKEISEVNNPETFDEHAQVAKQGGTIARNARMELEAKTGQAVVSPVNAKKILQIKNEQ
                     K"
BASE COUNT         1581 a         1523 c         1361 g         2061 t
ORIGIN      
        1 gggggaatta gtgagcattt ttcaatgtta tttatgtaaa ttatttgttt aaaattcttc
       61 aaacgtaaag aaaatctctc tttaatacca tcagttcccc ctttaggggg ttagggggca
      121 agtctgttaa ttagttcaat ttctcaaatt cctgcatgca agccaccaac gcttccacac
      181 tttcgagcgg acaggcattg taaatggatg cacggaaacc tcccactgag cgatgacctt
      241 tgatgcccac catgccacgt tctttggcaa atgccatgaa atcagcctct ttgtccttgt
      301 attcttcggc cattacgaag caaacgttca tgatggagcg gtcttctttc acagcagttc
      361 ccacaaacat tttattgcgg tcaatttcat cgtaaagcag attggctttc tgcatgttga
      421 gtttatgcat ggcttccacg ccgcccaggt ctttgaccca tttcagtgtt tcgtgcatta
      481 cgaaaataga aaataccggc ggagtattga acatggaatt gtcttttgga gtttcgccaa
      541 tgtgggttct gtaatccacc atagtttgca gtttgcggtc tgccttgccc aaaatatcct
      601 tccgtacaat tacaaatgca actccggcag gacccacatt tttctgtgct ccaccgtaaa
      661 taagtgcata tttgcttaca tccaccggcc gcgacatgat atcggacgac atatcggcca
      721 ccaaaggaac gggactggtt atatcttcat gaatttcagt tccgtaaatg gtattgttgg
      781 ttgtgatgtg gaaataatca acatcggtgg gaatggtgta acctttggga atgtaggagt
      841 aatttttatc ttcggacgat gccaccactt ctacttcgcc gtagaatttg gcttccttga
      901 tggctttctt agcccataca cctgtttgca gataagctgc ctttttgttc aacaagttgg
      961 ccgccaggta aaagaactga gtggaagcgc cgccgcccag aaacaaaacc gcatattcat
     1021 cagggatgtt gagcaggtct ctccacaact gttcggtttc ggccataatc cgctcccaac
     1081 cgggtgtgcg gtgcgaaatt tcaagaattc cgatacctgt tccatcaaaa tcgcgaatag
     1141 catcaatggc tgcttcaata gccggtttgg gcaaaacaca tggtcctgca ttaaaattat
     1201 gttttttcat acttgaaatt ttatgaatta tacttcagat taatgccaca aaattaagga
     1261 ttttattctg taataacctt acccggaaaa gaatatttag tcaaaatatt tggtttttgc
     1321 atgaacatac aaccgacttt cattcccgga attaaaaaag cagaattctg accgatttct
     1381 gcttttaatt tctactttat tttgtctgaa ttctgataaa ttttaaaatc tcttccaacg
     1441 cttttggcga gaatgtttgt tctattttag catattcagc aggagaaccg gtagtgcttt
     1501 cctgaaaaag atgatttaac ccgggaagtt ccactgccgt gagttttttg tttccgcttt
     1561 cgctgaatgc tttccggatg gcagccagat tttcggcaga aggaacctgc aggtctttag
     1621 aaccgttaag tgccagagtt gggcatttca cttttttgag catcggaaca ggatcgtatt
     1681 tgataaaaaa ctgcatccag ggcgaagaaa gctggtcggt atatgccttt acctgcatgt
     1741 cggattgttc ctgagttgca ttctctgtta atcccggaag gtcttttatg ttatttagaa
     1801 aataattttt caaatcggtt ttcagtttag ttgtgtcatt tgaattcagc acaatatcat
     1861 aagccccgga attgagtttg attgttttat ttatttcctg ttcgctcatt ccataggctt
     1921 tgctgattgc ttcggtttga agtacaagca gttctttgcc ccgcatgccc gggccagcaa
     1981 gcatcacaat gaatgccgct tttttgctgc ggacagccac catcggagca attagtccgc
     2041 cttcgctgtg acccatcagc ccgattttct tcttgttgat ttcttttcgg gtggaaagat
     2101 aacgaacggc agctttggtg tcatttgcaa aatcgacaga agtagcgttc tgaaaatcac
     2161 cggtggattg tgcagttccg cggtcatcgt agcgcagcac tccaatgcca tttcgggtga
     2221 gaaaatctgc aatcaccaga aaaggtttgt gccccatcag ttcctcgtcg cggttttgag
     2281 ggccgctgcc ggtgatcatc accaccacag gaaattttcc ctcttttttg ggcaatgtga
     2341 gtgttccggc cagcgtatcg ccgttaggat tggtaaattt cacctcctca gagtaataag
     2401 gataaggttt ctgcggctct tgcggacgca gtactttttc cttttctacc ggttgagccg
     2461 aaagatccag cgaaaatgac atgcccattt gggtaaaagt ccctttgatc actttgtcct
     2521 gaccaagaac accggtatat gttacgccta tcattttcac ctcaaatttc agggtgtcgt
     2581 tggcgaaagt cacccgatca accggaatgc cttttgcacc ctgatcggga ctgtccatag
     2641 tggctttata gccgttttcc gcttgctgaa tattaatcac caggcgaagt tgctgaccct
     2701 gcactttcag aattccgttc caggaaccgg taatattctg agcaaatgtc gacaaagaaa
     2761 tcaggaatga cagtaagata aatgttgtct ttttcattgg aaatatcttt ataataaatt
     2821 ttagttataa aaaatcctat taaatttaac ttacatagaa taaatcatca aaaaacctta
     2881 cttccaaaaa atcccccctt tagggggtta gggggctggt ctatttcccc gttatgatcc
     2941 tcttcacctc attcagttta ttcaacgctt ctatcggggt taaattattc acatccagcc
     3001 cttttatttc atcgcggatt tgttcgagta ccggatcatc cagctgaaag aaactcagct
     3061 gatagccttc gcgctgcgtg gctaattcac caaccggttt actcagatcg ccgttttggc
     3121 ggttgtcggc ttccagctgt acaagtattt tttcggccct tttcacaatg ctttgcggca
     3181 ttccggccat tttagccaca tgaataccga agctgtgttc actgccaccc cgcaccagtt
     3241 tacgtagaaa aatcactttt ttatccactt ctttcaccga aacattgtag tttttaatcc
     3301 gcgaaaaaga gttttccatt tcgttcagtt cgtgataatg ggttgcaaaa agcgttttgg
     3361 ctttgcagct ggcgtgctcg tgaatgtatt ccacaatggc ccaggcgatg gaaataccgt
     3421 cgtaggtact tgttccgcgt cccagttcgt cgaagagcac cagactgcgg tctgataggt
     3481 tgttcagaat gctggcggct tcgttcattt ccaccataaa cgtactttcg ccctgcgaga
     3541 tattgtcgct ggcacccacg cgggtaaata ttttatccac caccccgatt tttgcacttt
     3601 ccaccggcac aaagcagcca atctgagcca tgagcgtaat cagtgccgtt tgccgcagca
     3661 gtgccgattt tcccgacata ttgggaccgg taacgatgat gatttgctgg gtttttccgt
     3721 cgagatatac gtcgttggcc acatacgttt cgccgatggg cagttgtttt tctatcaccg
     3781 gatggcgtcc ctgccggata tcaatcacat cgctgtcgtt gatttcgggg cggacatatt
     3841 tattttccga agatactttg gagaaagaca gcaagcaatc gagctgagct atcagattgg
     3901 aattaagctg aatagcagca atgtaatcgg tcagactgat caccagttcg ttgaaaagcc
     3961 gtgtttcgat ggcaagaatt ttttcctcgg cacccagaat tttttcttcg tattctttca
     4021 gttcctgcgt gatgtaacgc tcggcgttca ccagcgtttg cttgcgtatc caggtttcag
     4081 gcactttgtc cttgtgcata ttgcgcactt caataaaata tccgaatacg ttgttgtaac
     4141 ttattttcag ggaaggaatg cccgtagttg cactttcgcg ttgctgaatt tcaagcagat
     4201 agcttttgcc cgaatgagcc agccggcgaa gttcatccag gtcggtattc acaccgctgc
     4261 gaataatgtc tcctttattc accaaaacgg gcggattgtt ttgcaattcc gcttctattt
     4321 tctccgaaat aactttgcag gggttcagct ggtcggcaat ttttctcagc gtcacatttt
     4381 ccacctccgc acaggcattt tttatgggtt caatagcctg caaagccact ttcagctgca
     4441 ccacttcgcg cggattgatg cgcccgacag ccactttgga tataattcgt tccaaatcac
     4501 cgattagtgc aatctgctgt tccagcatgt gttttaactc cgtatccctg aaaaaatact
     4561 ccaccacact gagccgttct tcaatgggtt tcacatcttt gagcgggaat gccacccagc
     4621 gtttcagcaa gcggccgccc atggggctga tagtccggtc aatcacatcc accagcgttc
     4681 ggccgccctc gttgatggtg ccgtagagtt ccagattgcg caccgtgaaa cggtcgagcc
     4741 acacaaaacg ctcctcctcg atgcgggtga ggtgggtaat atgttcggtt tggtggtggt
     4801 gggtcatgtc caggtaatgc agaatggcac ctgctgccac tattccgttt ttcagatttt
     4861 ccacaccaaa ccctttcaga tttttggtct gaaaatgttt aagcagcctt tcatttgcag
     4921 tttcgggaga aaaaccccag tcttcgaggg cgaaagtgaa atattttgta ccaaaatttg
     4981 cttcaaattc actccgtttt gagcggtcgt aaatgacttc cttgggcgaa aaactgctca
     5041 acagtttatc cacgtaatca accgtgcctt cggctatcaa aaattcaccc gttgaaatat
     5101 caagaaaaga tactcctgcc tgatttttat tcagataaac actggccaga aaggtatttt
     5161 ctttttgcgt aagcgtagta tcgctgtacg aaacgccggg cgtaaccagt tcggtgacac
     5221 cgcgtttcac tatttttttt gtcagtttcg gatcttccag ctggtcgcag atggcaacac
     5281 gtttgcctgc tctgacgagc ttgggcagat acgtatccag tgcatggtgc gggaaaccgg
     5341 ccatttccac cgttttggcc gaaccgttgg cacggcgggt gagcgtaatg cccagaattc
     5401 cggcgcattc cactgcatcc gtagaatatg tttcataaaa atcgccgcaa cgaaaaagca
     5461 gcaccgcatc gggatatttt cgcttaatat cgaaaaactg cttcatcatc ggggtttcaa
     5521 ccacgtcgtt tgtcatttat tatatgttgt gaactatttc tgttcgtttt taatttgtaa
     5581 aatcttttta gcattcacag gacttacaac agcctgaccg gtttttgcct ccaattccat
     5641 tcgggcattt cgggctatcg taccgccttg tttagcaact tgagcgtgtt cgtcgaaagt
     5701 ttcgggatta tttacttcag aaatttcttt cgtggaaagc tcagccagca tattcagcac
     5761 taattccttg ttagtcatat tgtcacgcag attttccttt ttcagccctt tgaattgttt
     5821 gtattctttg gccgttttgt cagcccacgt tttgtaaata atatctgtca gtgttgcaaa
     5881 ttgaattcct tcctccaaac cgcgtttttt ccattcatcg gtcaggtctt tacgaatttc
     5941 aatactttta agccgttgat ttatccagtt gtcagaatat cccaatcgtt tgtaatccat
     6001 catcgcctgt tctatggata attcaggatc ctgcatttgg tctaaacgct ctttggctac
     6061 ctgcgcaatc cacaatttga atggttctgc tttgggcgaa ggtatggatt gaataaggcg
     6121 gaaaagttgc tgagttgtag ctacatctgt taaccttttg cgtccatcac tcgcatccat
     6181 tttcaactgg ctacaatttg tagccgtttg atttccttct tttttaagtc gagtttttaa
     6241 tacactccaa tattttctgg gattttcgct ctctgtaagt acctcaatca catccactac
     6301 cgagaaatac cattcttcgg cttcatcatc ccaaactgta cgaactttct tttcttcaaa
     6361 aagttttatg gcattttgtt ttgtcatggc cttaaatttt atttaccgtt tccaccatca
     6421 acttccagtc gtactttttc ttttcttctt tcaaattttc gataaattta gtggagcgat
     6481 tattttcaaa aaaatcgacc agcgcatccg caattttgct cacttc
//
LOCUS       DAOPZK010000108         6446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_14726_length_6446_cov_21.977468, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000108 DAOPZK010000000
VERSION     DAOPZK010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14726_length_6446_cov_21.977468"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKH68_07010"
     CDS             complement(<1..503)
                     /locus_tag="PKH68_07010"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNX12724.1"
                     /translation="MKEFINENFLLQTETAQKLYHEHAKLQPIIDYHCHLNPEFIAND
                     RKFDNLGQIWLEGDHYKWRAMRTNGVDERYCTGKDTSDWEKFEKWAETVPYTMRNPLY
                     HWTHLELKSAFGVTKLLNPQSAREIFDICTEKLHKPEYSARGLMKLYNVETVCTTDDP
                     VDSLEYH"
     gene            complement(519..1748)
                     /locus_tag="PKH68_07015"
     CDS             complement(519..1748)
                     /locus_tag="PKH68_07015"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HNX12725.1"
                     /translation="MKKEFRKISLSSLVLFSLLLGAQTVAAQMVTVSNKSAKALKDYT
                     VEVPLSKFSLPAGSYIAVSTENEIVPLEISTDLKGNQTAIFPVAEIKPFSTLNYSIIK
                     GSADKYPKRTYAEIAHKIGGKFVGKIYEGGFSWVKPNYMRVPDNFTDHAYYIKYEGPG
                     WESDKVAFRFYLDWRNGIDVFGKKTPGIVLPFVGTEDYEKYHSMADWGMDNMKVGNSL
                     GIGSIAVWNGTKAMRVAKTDSVISYIPADGKIRSQVMTTYYGWDANGVKCNLKSLISI
                     DAGSRASHMELSVDKSLVNIATGIVKDKNAALIKSDNPDSEWSYIATFGKQSLNNDMQ
                     GLAVFYRTKQLQKVTEDELNHVVVLNPDNGRVEYYFMPTWELDWEPVADKAAFMKCVN
                     EVLDRLNNPVTLKTKKK"
     gene            complement(1962..5297)
                     /gene="mfd"
                     /locus_tag="PKH68_07020"
     CDS             complement(1962..5297)
                     /gene="mfd"
                     /locus_tag="PKH68_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNX12726.1"
                     /translation="MLNLSDFQQIYARHPQVEALTLWAATHEQNVKISGLCGSSLAVV
                     AASLFVKAPHMQVIAMNDADEAAYLYNDLRQILSDEKVLFFPSSYKKAIRLSQLDSSN
                     EILRTEVLNRLVNSSAPCVIVTYPEALIQKVISSQGIKTRTIRLRTGESIGMDFLAEM
                     LAEYGFERVDFVYEPGQYSIRGGIMDIFSYSFEMPYRIDFFGDEVESIRVFDIETQLS
                     QEKKQQIEIVPDLHKGDKSTRLVSFTDFVPRDTWFQFTNAGFIRDRINQLYDEALVKA
                     NSGERIVENLHSSNVTGDEFLQGVEPFRKLEWANKSYFKTKTTVQFNTSPQPVFQKNF
                     DLISDNLKKNQAEGYRIFIFSDSVKQTDRIAAIFADRDDKISFEPIKNTLHEGFIDHD
                     LSVVCYTDHQIFDRFHKYQLKTDKTRQGKVVMTLKELNQFQQGDYMVHVDHGVGKFGG
                     LVKTNVNGKMQEMVKLIYKDDDIIFVSIHSLHRISKYKGKEGEAPRINKLGSGAWERL
                     KERTKSKVKDIARELIKLYAKRKAEAGYQFSPDSYMQQELEASFLYEDTPDQVKSTAD
                     IKKDMESEMPMDRLVCGDVGFGKTEVAIRAAFKAVADSKQVAVLVPTTVLATQHYHTF
                     RDRLKDFPCTISYLSRAKAPKQAKETLERLANGDVDIIIGTHKLVGKTVKFKDLGLLI
                     IDEEQKFGVTVKERLKELKVNVDTLTLTATPIPRTLQFSLLGARDLSIINTPPPNRYP
                     VQTEIHAFDEDIIREAIQIEMNRNGQVFFVNNRIQNIYLIEAMIKRLVPGCRVAVGHG
                     QMPAEKLEEIIVDFIDYEYDVLVATTIIESGIDIPNVNTIIINSAHKFGLSDLHQLRG
                     RVGRSNRRAYCYLLAPEMSLLTPDARRRLQAIETFSELGSGFNIAMQDLDIRGAGNML
                     GAEQSGFIADLGYETYQRILNEAVHELKDEEFAELYADERTENKGNKVYVTDCQIDTD
                     LELMFSADYIENVSERINLYRELDNLENENELQAFRSRLEDRFGKLPQQASDLMLIVR
                     LRWLAMHYGIEKLILKNEQMIAYLVSNLKSTYYQSDEFGRLLQYMASHPRVCKLREQN
                     GKRSVVFMDVKSIDKAWAIFESI"
     gene            5527..6207
                     /gene="aqpZ"
                     /locus_tag="PKH68_07025"
     CDS             5527..6207
                     /gene="aqpZ"
                     /locus_tag="PKH68_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNX12727.1"
                     /translation="MKRNLAEFFGTFWLVLGGCGSAVLAAGVPDVGIGYAGVSLAFGL
                     TVLTGAYGLGHISGAHFNPAVTFGLWAGGRFKGKDILPYVISQILGALLAAAVLYTIV
                     TTSGMEIGRFAANGYGELSPNGYSLTAGLLTEFVMTFMFLLVILGATDERAPKGFAGI
                     AIGLALTLIHLISIPVTNTSVNPARSISQAVFVGGTYITQLWMFILIPIAGAMCAGLV
                     YKFFKADK"
BASE COUNT         1660 a         1410 c         1377 g         1999 t
ORIGIN      
        1 atatgatatt ccaggctgtc aaccggatca tcggtagtac aaactgtttc gacattgtac
       61 agtttcatca gtccgcgtgc cgaatattcg ggcttatgga gtttttcggt acaaatatca
      121 aatatttcac gggcagattg cggattaagc agttttgtta ctccgaaagc gcttttcagt
      181 tccaggtgtg tccagtgata cagtggattg cgcatggtgt agggaaccgt ttctgcccat
      241 ttttcaaatt tttcccagtc ggatgtgtct tttccggtac aatagcgttc atccacgccg
      301 ttggtacgca ttgcccgcca cttgtaatga tcaccttcga gccatatctg acccagatta
      361 tcgaatttcc ggtcattggc tataaattca gggttcagat ggcaatgata atcgatgatt
      421 ggttgcagtt ttgcatgttc atgatacagt ttttgggctg tttcggtctg caataaaaaa
      481 ttttcgttta taaattcttt catttttcag gagataattt atttcttttt tgtcttcagt
      541 gttaccggat tgttcagtcg gtcaagcact tcattcacac atttcatgaa agcagcttta
      601 tcggcaacag gttcccagtc gagttcccag gttggcataa aatagtattc cacccttccg
      661 ttatcgggat tgagcacaac cacgtggttc agttcatctt cggtgacttt ttggagttgt
      721 ttggtacggt aaaatacggc aagtccctgc atatcgttat tgagactttg ttttccgaaa
      781 gtggctatat atgaccattc gctgtcggga ttatccgatt tgatcagtgc agcattttta
      841 tcttttacaa tgccggtggc aatatttacc agggatttat ccaccgaaag ttccatgtgc
      901 gatgcacggc tgcctgcatc gatggaaatc agcgatttca ggttgcattt cactccgttg
      961 gcatcccagc cgtaataggt ggtcatgact tgtgagcgga tttttccatc ggcaggaatg
     1021 taactaatca cgctgtcggt tttggccacc cgcatagctt ttgttccatt ccacacggct
     1081 atagaaccta ttcccagcga gttgcccact ttcatattgt ccattcccca gtcggccatg
     1141 ctgtgatatt tttcgtaatc ttcggttccc acaaatggta gtacgatgcc cggagttttt
     1201 ttcccaaaca catctattcc gtttcgccag tcgaggtaga agcgaaacgc caccttatca
     1261 ctctcccagc ccggaccttc gtatttgatg taataggcat gatcggtaaa attatcggga
     1321 acccgcatgt aattgggttt tacccacgag aaaccgcctt cgtatatttt cccgacaaat
     1381 tttccgccaa tcttgtgtgc aatttcagca taggttcgtt tgggatattt gtcggcgctt
     1441 ccttttatta tagaataatt taatgtggaa aatggtttaa tttccgcaac gggaaaaata
     1501 gcggtttgat ttcctttcag gtcggtactg atttcgagtg gaacaatttc attttctgta
     1561 gaaacagcaa tgtagctgcc tgccggtaaa gaaaattttg acagaggaac ttcaaccgtg
     1621 tagtctttca gtgctttggc cgatttgttg cttactgtga ccatttgagc agcaaccgtt
     1681 tgcgcaccca aaagcagtga aaataaaacc agggaggata aactgatttt tcggaattct
     1741 tttttcattt tttcagtgat gatttgtttt cgaaaaaaac ctgattacta taatttgaaa
     1801 ataatcacaa atatagactt ctttttgaag aatacatagg taattaatga caataatgta
     1861 tcaaattttg ataaaatcag aatatcaccc taaaaaaacc aatctgactt acctcccatt
     1921 tacagtaaga ggttttgttg tgaaattgat taagaaagca gttaaatgct ttcaaaaatc
     1981 gcccacgctt tgtctatcga cttaacgtcc ataaaaacca cagaacgctt gccgttttgt
     2041 tcacggagtt tgcacacacg cgggtggctc gccatgtatt ggagcaaccg gccaaactca
     2101 tcggactggt agtaggtcga tttcagattg gataccagat aggctatcat ctgctcgttt
     2161 ttcagtataa gtttttcgat gccataatgc atggccagcc atcgtaacct gacaatcagc
     2221 atcaggtctg aagcctgctg tggtagtttt ccaaagcggt cttcgagccg tgaccggaat
     2281 gcctgcaact cgttctcgtt ttccaggttg tccagttcgc ggtacaaatt gatacgttct
     2341 gagacatttt caatgtagtc ggctgaaaac atcagctcca ggtcagtgtc aatctggcaa
     2401 tcggttacat agaccttatt tcctttattt tcagtccgtt cgtcggcata tagctcggca
     2461 aattcttcgt ctttcaattc atgcactgct tcgttaagta tccgttgata ggtctcatag
     2521 cccagatcgg cgataaatcc gctttgctcg gctccaagca tatttccggc tccacgtata
     2581 tcgaggtcct gcatggcaat attaaatccg cttccaagtt cggagaaggt ttcgatagcc
     2641 tgcaacctgc gccgcgcatc gggagtgagc aggctcattt caggcgcaag caggtaacaa
     2701 taagctctgc gattactgcg ccctaccctg ccccggagtt gatgcaaatc gctcaaacca
     2761 aatttatggg cactattaat aatgatagta ttcacattcg ggatatcaat gccggactcg
     2821 atgatggtag tggcaaccag tacatcgtat tcataatcaa taaaatcgac aataatttcc
     2881 tcgagcttct cggcaggcat ctgcccgtga ccaaccgcta cgcggcagcc cggcaccagt
     2941 cttttgatca ttgcttcaat caggtaaata ttttggatgc gattattgac aaagaaaacc
     3001 tgaccatttc ggttcatttc tatctgaatg gcttcccgga taatatcttc atcgaatgca
     3061 tgtatttcgg tttgaaccgg ataacggttt ggcggtggtg tgttgataat cgacaaatcg
     3121 cgtgccccga gcagcgaaaa ttgtaatgtt cgtggtatgg gtgtagctgt aagcgttagc
     3181 gtatccacgt tcactttcag ttctttcagt cgttccttta cggttacacc gaatttctgt
     3241 tcttcatcga ttatcagtaa tcccagatct ttgaatttca cggttttgcc caccagtttg
     3301 tgagtgccaa taataatgtc gacatcaccg ttagccagcc tttcgagagt ttctttggcc
     3361 tgcttgggcg ctttggcacg actcagataa gaaatggtgc acggaaaatc tttcagccgg
     3421 tccctgaaag tatggtaatg ctgcgtggca agcaccgtgg taggaaccag cacggccacc
     3481 tgcttgctgt ccgctacggc tttgaaggct gcgcggatag ccacttccgt ttttccaaaa
     3541 cccacatcgc cacaaaccag ccgatccatg ggcatctccg attccatgtc cttttttata
     3601 tccgctgtag atttgacctg atcgggagta tcttcataga gaaacgatgc ttccaactcc
     3661 tgttgcatgt agctgtccgg tgaaaactga tagccggctt cagctttgcg tttggcgtag
     3721 agttttatca attcgcgcgc tatatctttt actttggatt tggtacgctc tttaagccgt
     3781 tcccaggcac ccgagccaag tttgttgatg cggggagctt caccttcttt tcctttgtat
     3841 ttggaaatgc ggtgtaatga atgtatgctc acaaaaatga tatcatcatc cttgtaaatc
     3901 aacttcacca tttcctgcat tttcccattc acattggttt tgacgagacc gccgaacttc
     3961 cccaccccgt gatccacatg caccatgtaa tcaccctgtt gaaactggtt cagttccttg
     4021 agcgtcatca ccactttgcc ttgtcgggtt ttatctgttt tcagctgata tttatgaaaa
     4081 cggtcaaaaa tctgatgatc tgtgtagcat accaccgata aatcatgatc aataaagcct
     4141 tcgtgcaatg tgttttttat cggttcgaat gaaattttat catcgcggtc ggcaaagatg
     4201 gctgcaatac ggtcggtttg cttcacgctg tcgctgaaaa taaaaatgcg gtatccttcg
     4261 gcctggtttt ttttcaggtt atcgctgatg agatcaaagt ttttttgaaa aaccggctgc
     4321 ggagaagtat taaactgtac ggtggttttg gtcttaaagt atgatttatt agcccattcc
     4381 agttttcgga aaggctcaac accctgtaaa aattcatcgc cggtaacatt tgatgaatgt
     4441 agattctcaa cgattcgttc accactgttg gctttaacga gtgcttcgtc gtacagttgg
     4501 ttgatacgat cgcggataaa acctgcattg gtaaactgaa accaggtatc ccggggtaca
     4561 aaatcggtaa atgaaacaag cctggtggat ttatcgccct tgtgcaggtc cggtacaatc
     4621 tctatctgtt gttttttttc ctgcgaaagt tgtgtttcaa tatcaaaaac ccggatcgat
     4681 tccacctcgt cgccaaagaa atcgatgcgg taaggcattt caaaggaata ggaaaatata
     4741 tccatgatgc cgcccctgat ggaatactgg ccgggttcgt aaacaaaatc aacgcgttca
     4801 aatccatact ctgccagcat ttctgccagg aaatccatgc cgatactttc gcctgttctc
     4861 agtctgattg tccgcgtttt aataccctgt gatgatatta ccttttgaat taatgcctcg
     4921 ggataagtga ctataacaca cggagctgag ctgttaacaa gccggttgag cacttcggtg
     4981 cgtaatattt catttgaaga gtctaactgc gacaaccgga tggctttttt gtaagatgat
     5041 gggaaaaaca atactttttc atccgaaagg atttgtctca ggtcgttgta aagataagct
     5101 gcctcatcgg catcattcat tgcaatcacc tgcatgtgag gcgcttttac aaaaagtgaa
     5161 gcggcaacta ctgccaatga agaaccgcac aaaccggata ttttcacatt ctgttcgtgt
     5221 gttgctgccc acaaagtcag cgcttctact tgcggatgac gggcataaat ctgctgaaag
     5281 tcggaaaggt tcaacatgta atcaagtgca aagatacgat taaatgaaga atttgaaaac
     5341 gcattcagtt ttttaagttt gattattgat catattaatg gctaatacat taaaaattaa
     5401 tcaaaataca tttttttctc aaacatattt ttacctttgt tttttcaaaa aaaattgtct
     5461 attaaaaata aagtacggag ataaccgaaa atccttacga gtaggtataa attaaaaaca
     5521 aaagttatga aaagaaactt agcagaattt tttggcacat tttggttggt actgggcggc
     5581 tgcggaagcg ctgtactggc tgcaggtgtt cctgatgtag gcattggcta tgcaggtgtg
     5641 tcacttgctt tcggtcttac cgttctgacc ggagcttacg ggcttggaca catttcgggt
     5701 gcacatttca atccggcagt gacctttggt ttatgggccg gtggccggtt caagggtaaa
     5761 gacattctac cttatgtaat ttctcagatt ttaggtgcat tacttgccgc tgcggtactt
     5821 tataccattg taactaccag tggaatggaa attggtcggt ttgcggcgaa cggatatggt
     5881 gaactttcgc ccaacgggta ttcactgaca gccggtttgc tcaccgaatt tgtcatgaca
     5941 tttatgttcc tgctggtgat actgggagca accgatgaac gtgcacccaa aggttttgcc
     6001 ggtattgcca ttggtttggc actcaccctc atccacctca tcagcattcc tgtgaccaat
     6061 acgtcggtca atcctgcacg gagtatcagt caggctgtgt ttgtgggagg cacttacatt
     6121 acacaactct ggatgtttat ccttatcccg atagccggag caatgtgtgc cggattggta
     6181 tataagtttt tcaaagctga taaataaaaa aacttcctga attaaaaaac ggggttgaca
     6241 atgaatgttg tcaaccccgt tttttttatg gaaaaattac tattgtccgg tattgtaatg
     6301 tgttcaactt ttgcaagcta ttatcagtct ataaatcaga aaaatacgaa ttaattacat
     6361 ctgacaatca gatagatata cgttttttca caaacagggg cttccgcccc ttaaaccccg
     6421 acttactttt tgtcttgaaa caaaaa
//
LOCUS       DAOPZK010000109         6441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_14745_length_6441_cov_27.958660, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000109 DAOPZK010000000
VERSION     DAOPZK010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14745_length_6441_cov_27.958660"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PKH68_07030"
     CDS             complement(<1..617)
                     /locus_tag="PKH68_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNX12728.1"
                     /translation="MKISSSLKEQISMLPENPGVYQYFNANDEIIYVGKAKNLKKRVS
                     SYFNKIHDNGKTNVLVKNISYLRYIVVDSEENALLLENNLIKQYQPRYNILLKDGKTY
                     PSICITNEEFPRIFKTRNINKKWGQYFGPFSSGYTINSILEIINKLYPIRSCHLALTE
                     QSIATGKFKVCLDYHIKKCKGPCEAYQTRKEYQDNINEIKKIIQG"
     gene            complement(742..>1980)
                     /locus_tag="PKH68_07035"
     CDS             complement(742..>1980)
                     /locus_tag="PKH68_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX12729.1"
                     /translation="LTEQEGENDFHKFSFLKEVQRELPQQSCWITYTNKHTHEILHSG
                     LEFSPLYNGQIQSIGPRYCPSIETKIITFADKNAHQLFLEPEGVNSREYYVNGFSSSL
                     PLQTQIDAIQSVKGLEKAQLYRPGYAIEYDFFDPTQLKHTLETKLIKNLFFAGQVNGT
                     TGYEEAGGQGLIAGINAHINCHGGNEFILHRDEAYIGVLIDDLVTKGVDEPYRMFTSR
                     AEYRLLLRQDDADVRLTKRSYDLGLVKKNRYEQLQNKKSEQEKLIGFLKHNSVKPKDV
                     NEFLVAHNSTALEHGCKLSDLILRPQFGLSDLAEAVPALQEKLENISEIREEIIESVE
                     VMLKYAGYIEREKLIAEKLQRLEHIIIRDKIDYSTVQSLSTEARQKLARINPETIGQA
                     SRIPGISPSDVNILLVMMGR"
     assembly_gap    1981..2080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2081..2334)
                     /locus_tag="PKH68_07040"
     CDS             complement(<2081..2334)
                     /locus_tag="PKH68_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX12730.1"
                     /translation="MDFNYDVIVIGAGHAGCEAATASANLGSKTLLITMDMNKIAQMS
                     CNPAVGGIAKGQIVREIDALGGQMGIVTDKSAIQFRMLNRS"
     gene            complement(2487..2714)
                     /locus_tag="PKH68_07045"
     CDS             complement(2487..2714)
                     /locus_tag="PKH68_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12731.1"
                     /translation="MDTTELVGYIAMAFLVVSFLPKQIRKIRLINFIGCSLFVIYGIL
                     LGWKWPLIVSNGLIALIQIYYLFIAKNKIVD"
     gene            complement(2829..3155)
                     /locus_tag="PKH68_07050"
     CDS             complement(2829..3155)
                     /locus_tag="PKH68_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX12732.1"
                     /translation="MSTLFNNGEVFPLETSVEYTSGGVVSKQITKSPAGNVTLFSFDK
                     GQGLTEHTASFDAMVQIIDGVAEIHIDGIPRLVKKGECIIMPANHPHALNAVEPFKML
                     LIMIKG"
     gene            complement(3295..3567)
                     /locus_tag="PKH68_07055"
     CDS             complement(3295..3567)
                     /locus_tag="PKH68_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNX12733.1"
                     /translation="MEIKAQSIKELRQIPGVGPSIANDLYRIGIRKIEDLKFKNPEDL
                     YDWSNQMAGCVQDRCLLYVFRCAVYFAETEPENREPEKLNWWNWKI"
     gene            complement(3669..4676)
                     /locus_tag="PKH68_07060"
     CDS             complement(3669..4676)
                     /locus_tag="PKH68_07060"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNX12734.1"
                     /translation="MKVAIVGASGAVGQEFLRVLEERDFPLTELVLFGSSRSAGKEYE
                     FKGKKLVVKELVHGDDFKGIDIAFTSAGAGISKEFAADITKFGAVMIDNSSAFRMEND
                     IPLVVPEVNAADAKDRPRGIIANPNCTTIQMVVALKALEDISHIKRVHVSSYQAASGA
                     GAAAMDELALQYTQIANGEEPTVSKFAYQLAYNVIPHIDVFTDNGYTKEEMKMYFETR
                     KIMHSDVEVSATCVRVPTLRAHSESVWAETERPISVEEAREAFSKAQGVVLMDNPAEK
                     IYPMPLFLSGKDPVHVGRVRKDISNPNGITFWCVSDQIKKGAALNAVQIAEYLIAEKD
                     I"
     gene            4797..>5167
                     /locus_tag="PKH68_07065"
     CDS             4797..>5167
                     /locus_tag="PKH68_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12735.1"
                     /translation="MKKSLIILIILLPFYAFSQINETFSDGNFTQNPVWTGTTENFKV
                     NTQFQLQSQAATTSKSYLSTPSEAFEDAVWEFWVKIAYTTSSSNYASVYLTSDRADVS
                     ADCYGYYVQVGNTNDEISLYR"
     assembly_gap    5168..5267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5268..5819
                     /locus_tag="PKH68_07070"
     CDS             <5268..5819
                     /locus_tag="PKH68_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12736.1"
                     /translation="SFLITNATGDSIFDEVKYDVKWHHPMVRITAGVSLERINPSLPS
                     QNPDSWHSAASEVYYGTPGYRNSQFRDISTAEIPEKWVWIEPESFTPDNNGMDDICLI
                     RYKTEAGGFTANVLIFNSVGVRVRQLAANQLLSADGFLTWDGRTDKGKNINPGIYMLY
                     FEVFNAESGVRKVEKLPVVVSAR"
     gene            5912..>6441
                     /locus_tag="PKH68_07075"
     CDS             5912..>6441
                     /locus_tag="PKH68_07075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNX12737.1"
                     /translation="MDKVSYALGLSLGNNLISSGITSLNISKLSQGIKDVLEQNQPEI
                     SYQEAQDVINEYFQNLQSKMSAKTIEEGKAFLESNGKRPEVVTLKSGLQYEVIREGNG
                     PIPNAKNTVKVHYHGTLIDGTVFDSSVRRGEPAAFGVTQVISGWVEALQLMPVGSKWK
                     LYIPSDLAYGAQGAGQ"
BASE COUNT         1748 a         1255 c         1228 g         2010 t
ORIGIN      
        1 tcgccctgaa taattttttt tatttcgtta atgttgtcct gatattcttt tctggtttga
       61 taagcctcgc aaggaccttt acattttttt atgtgataat cgagacacac tttaaatttt
      121 ccagtcgcaa tgctttgctc ggttagagcc aaatgacagg atcggatagg gtagagtttg
      181 ttgataattt ccaggataga atttatagta taacccgaac tgaaaggtcc gaagtattgt
      241 ccccattttt tattgatgtt tcttgttttg aaaatgcggg gaaattcttc gttggtaata
      301 caaatactgg gatatgtttt accatctttc agtaaaatat tatatctcgg ttgatactgc
      361 tttataagat tattttcaag cagaagggcg ttttcttcac tatccaccac tatataccgg
      421 agataggaaa tattctttac caaaacattt gttttgccgt tatcatgaat tttattgaaa
      481 taggagctta cccttttttt taggttcttg gctttaccta catatattat ttcgtcgttt
      541 gcattaaagt attggtatac tccaggattt tccggcagca tactaatctg ttcttttaat
      601 gacgatgaaa ttttcataca accgaagcgt tccacgtgga acaaaatatt attaatcgat
      661 tattttacaa tatgatagtt tgaaatttga actcagggtt tctgagtaaa atttgatttt
      721 gttccacgtg gaacatggtt attatcttcc catcataacg agcaaaatgt tgacgtcgct
      781 tggagaaatt cccggaattc tgcttgcttg tccaatggtt tcggggttta ttctggcaag
      841 tttttgacgg gcttctgtcg atagtgactg aacggttgaa tagtcaattt tatctctgat
      901 gatgatatgt tcgagacgtt gaagcttttc tgcaatgagt ttctcgcgtt caatgtagcc
      961 tgcgtatttc aacatcactt ccacgctttc aataatttct tccctgattt ccgaaatgtt
     1021 ttctaacttt tcctgcaatg ccggaactgc ttccgccaaa tcagaaagac caaattgagg
     1081 tcgtaaaatc aaatcggaga gtttgcagcc atgttccaat gccgttgaat tatgtgcaac
     1141 caaaaactca ttcacgtcct ttggttttac agaattatgt ttcaaaaatc caattaattt
     1201 ttcctgctcc gattttttat tttgtagctg ttcgtaacgg ttttttttca ccaatcctaa
     1261 gtcgtaggag cgctttgtta aacggacatc ggcatcatcc tgccttagca ataagcggta
     1321 ttcggcacgg gaagtaaaca tgcggtacgg ctcatccaca cctttggtga ccaaatcgtc
     1381 aatcaacaca ccaatgtaag cttcatcgcg gtgaaggatg aattcattgc cgccgtggca
     1441 attgatgtga gcgtttatgc cggctatcaa accctgacct cctgcttcct cgtatccggt
     1501 agtgccattg acctgaccgg caaaaaacag attttttatc agttttgttt ccagggtatg
     1561 cttcaactgt gttggatcaa aaaagtcgta ttcgatggcg tatcccggac ggtaaagctg
     1621 tgctttttcc aggcctttta cactttgaat ggcgtcaatc tgagtttgca ggggcaggga
     1681 agaggaaaat ccgtttacgt aatattcccg tgaatttaca ccttccggtt cgaggaataa
     1741 ttggtgtgca tttttgtcgg cgaaagttat aattttagtt tcgatactcg gacaataacg
     1801 cggaccaatg ctttgtatct gtccgttgta aagcggtgaa aattccagtc cgctgtgaag
     1861 aatttcgtgc gtatgtttgt tggtataggt tatccagcaa ctttgctgtg ggagttcgcg
     1921 ttgtacttct ttcagaaatg aaaatttatg aaaatcgttt tctccttcct gttcggtcag
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gagcggttga gcatgcggaa
     2101 ctggatggcg cttttgtcgg tcacaattcc catttgtcca cccaaagcat caatttcacg
     2161 cacaatctga cccttggcaa tgccgcctac ggccggattg cagctcatct gtgctatctt
     2221 gttcatatcc atagtgataa gcagcgtttt cgatcccaga ttggccgaag cggttgctgc
     2281 ttcacagccg gcgtgaccgg cacctatcac tattacatcg tagttgaaat ccataaataa
     2341 ttcattttga gcctgcaaag gtaacaagtt tcgttgtttt ggcaataaaa ccaccccggg
     2401 cgttgaattt gattaacatt attgaataaa caaaacgacc ggaagcaaat gaatgtttcc
     2461 ggtcgcaaga atttttttat tttatttcaa tccacaattt tattttttgc aataaataaa
     2521 taataaatct gaataagtgc aatcagtccg ttggaaacaa tcagcggcca tttccatccc
     2581 aataatattc cataaataac aaataacgag caacctatga agttaattaa tcgaattttt
     2641 ctgatttgtt tcgggagaaa tgaaactact aaaaaagcca ttgcaatgta acccaccaat
     2701 tccgtcgtat ccatatttta ttgttataat ttattttggg ctgcaaagat acacctttta
     2761 acggaattga atgttaaata tgtaaaaaaa tacagaaatt gatatatcag gaaaatgaaa
     2821 accggatatt aacccttaat cataatcaac agcatcttga agggttccac tgcatttagt
     2881 gcatgcggat gattggctgg cataatgata cactcgcctt ttttaaccag tcgtggtatc
     2941 ccgtcaatgt gtatttcagc tactccatcg attatttgga ccatggcatc gaaggatgcg
     3001 gtatgttcag taagcccttg tcctttgtca aatgaaaaca gggtgacatt tcctgccgga
     3061 ctttttgtaa tttgtttact aacaacacct ccactggtgt attctaccga agtttccaaa
     3121 gggaaaactt cgccattatt gaataatgtg ctcattttct tttctttatc gtaagtaaat
     3181 ttcatattgc atatattttg atactaataa cgacatcata ttataaaaag ttcaaaacct
     3241 taagtcagaa gttaacggac tcatcaatgt ggccaggtat tattttcata acctttatat
     3301 tttccagttc caccagttga gtttttcagg ttcacggttt tcaggttcag tttcggcaaa
     3361 atagacagca caccggaaaa cgtatagcaa gcatcggtcc tgcacgcaac ctgccatctg
     3421 attggaccaa tcgtacaaat cttccggatt tttgaatttc aaatcttcaa tttttcggat
     3481 gccaattcgg tataaatcgt tggctatgga tggaccaact cccggtattt gccgtaattc
     3541 tttgattgac tgtgctttaa tttccatttt atttaaaata aaaaaactac aacagacact
     3601 taagctattt tcaaaatatt aaacttagaa aatgtaaact tatgtgcctg ttgtagttct
     3661 gtatagaatt aaatatcctt ttcggctatc aggtattcgg caatctgcac agcattgaga
     3721 gcagcgcctt ttttgatttg atcgctcaca caccagaatg tgatgccgtt ggggttggat
     3781 atatctttgc gcacacggcc tacatgtaca ggatctttgc cggataagaa aagtggcata
     3841 ggatatattt tttctgccgg attatccatc aaaaccactc cctgagcctt cgaaaatgct
     3901 tcgcgggctt cttctacgct gatcggacgt tcggtttcgg cccaaacact ttcggagtga
     3961 gcacgcaagg ttggtactcg tacgcaggta gcacttactt ccacgtcaga atgcatgatt
     4021 tttcgtgttt cgaagtacat tttcatttcc tctttggtat aaccgttgtc ggtaaataca
     4081 tcgatatgag gtatcacgtt gtaagccagt tgataagcga atttacttac ggttggttct
     4141 tcgccgttgg caatttgagt gtattgcaaa gcaagttcgt ccatggcagc tgctccggct
     4201 ccgctggcag cctgatagga tgaaacatga acgcgtttga tgtgcgaaat atcttcgagt
     4261 gctttcagtg ctacaaccat ctgaatggtg gtacagtttg gattggcaat tattccgcgc
     4321 gggcgatctt ttgcatcggc agcatttact tccggcacca ccaatggtat gtcgttttcc
     4381 atacggaatg cgcttgaatt gtcaatcatc accgcaccga atttggtgat atccgctgca
     4441 aattctttgg aaataccagc tccggctgag gtaaaagcga tgtcaatacc tttgaaatca
     4501 tcgccatgaa ccaattcttt cactaccagt tttttccctt taaattcata ttcttttcct
     4561 gcactgcgcg atgagccgaa caatacaagt tcagtcaacg gaaaatccct ttcctccaat
     4621 acccttaaaa attcctgtcc tacggcaccg ctggcgccta cgatagctac tttcatactt
     4681 ttatatttta atgtaatttc ttctaattta aaattataac tatatttgtc ttcttattgt
     4741 tctccctttc aaaatctctg caaaattaat tcatttcaac ttatttactg cattttatga
     4801 agaaatcgtt gataatttta attattctgt tgccttttta tgcattttcg cagataaatg
     4861 aaacattttc ggatggtaat ttcactcaaa atcctgtttg gacgggaaca accgaaaatt
     4921 tcaaggtaaa tactcagttt cagttgcaat cgcaggctgc caccacttca aagtcttatc
     4981 tatccacacc gtcggaagct tttgaagatg ccgtgtggga attttgggtg aaaatagctt
     5041 atactacctc gtcctccaat tatgcatctg tttatctcac atccgatagg gccgatgtat
     5101 ccgccgattg ttacggatat tatgttcagg ttgggaatac caacgatgaa atttcgcttt
     5161 acaggcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt tttctgataa
     5281 cgaatgccac aggcgacagt atcttcgacg aagtgaaata cgatgtaaaa tggcaccatc
     5341 cgatggtgag aataaccgcc ggcgtatcgc tagaacgaat taatccttca ttgccatctc
     5401 aaaaccccga cagctggcat tcggccgctt ctgaggttta ttacggaact cccggttaca
     5461 gaaattctca attccgggat atttccactg ctgaaattcc tgaaaaatgg gtgtggatag
     5521 agcccgaaag ctttacaccc gacaataatg gaatggatga tatctgtctg ataaggtata
     5581 aaaccgaagc cggaggattt acggccaatg tgttgatttt caattctgtt ggtgtgaggg
     5641 ttcgccaact cgctgctaat cagctgcttt ctgccgatgg ttttttaacc tgggatggcc
     5701 gaaccgataa aggtaaaaat atcaatccgg gcatatatat gctttatttc gaagtattta
     5761 atgccgaaag cggtgtccga aaagtcgaaa aattgcctgt tgtggtttca gcaagataat
     5821 tttaataaat taacatccta ttattcaaat aattcatcta tctttgcagt cattttttaa
     5881 cacaaaaatt ttataaaaat aacggaataa tatggataaa gtaagttatg ccctgggttt
     5941 aagtttggga aataatttaa taagcagcgg aataacatcg ctcaatatta gcaaactatc
     6001 tcagggaatt aaagatgtat tggaacaaaa ccaacccgaa atttcctatc aggaagctca
     6061 ggatgtgatc aatgaatatt ttcaaaatct ccaatcaaaa atgagtgcaa aaaccatcga
     6121 agaaggaaaa gcctttttag agagtaatgg caaacgcccc gaagtggtga cgctgaaaag
     6181 cggattgcaa tatgaagtaa tccgggaagg caacggacca attcccaatg caaagaatac
     6241 cgtaaaagtt cattatcacg gcacgttgat tgacggtact gttttcgaca gttctgtacg
     6301 tcgcggcgaa ccggctgcgt ttggtgtaac acaggtaatt tcaggttggg ttgaagcatt
     6361 acaattgatg ccggttggtt ccaaatggaa actttacatt ccttcggatt tggcatacgg
     6421 tgctcaggga gctggtcaac a
//
LOCUS       DAOPZK010000110         6398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_14890_length_6398_cov_22.499133, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000110 DAOPZK010000000
VERSION     DAOPZK010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14890_length_6398_cov_22.499133"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKH68_07080"
     CDS             complement(<1..533)
                     /locus_tag="PKH68_07080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX12738.1"
                     /translation="MEKYILITGGTKGIGRAVADILAQSGYKLILTYGSDEVQAAKVK
                     AEFQELYPASVEVLQADISEKQAIDTIENYLNTHDIHLFSVVFNAGLTCRTPFEQMTL
                     EEWERVFFANVHFPVFMLQRICSTLQPGGNVLFTGSLMGEYAHSVSLAYGVSKSAVHA
                     LVKNLVKFMQPYEIRVNA"
     gene            904..2091
                     /locus_tag="PKH68_07085"
     CDS             904..2091
                     /locus_tag="PKH68_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX12739.1"
                     /translation="MAEKKKIFGFTPNVFYTGIVSFLTDTSVKMVYSVMPMFLMSIGA
                     SKTSLSLIEGIAESTASLIKAFSGYWSDRIGKNKPFMLIGYGLSAVIMPVYALVVSPL
                     QVLILRFIERVGKGIRTAPRDSLVAGSVNGNEVGKSFGLHKAMDNSGAILGPLLAFLL
                     LLFHPGDFRLIFLLAGIPGLLGIFVLYFGIHEAKKHKESLLKKFRFSDYPKRFYLFLA
                     IIFVFTLGNSTDALLMVKANEVGIKVTFIPLVYLISSIVSVFLAIPFGQLSDRIGREK
                     LLIAGFFIYAMVYFGFGITANVYVIVALFAFYGIYSALTDGQQKSMISDLVDENKKGT
                     GMGIYNALLGITLLPASLIAGLLYDKVNSKIPFYFGGSMALLAIVLMVIYLATKPKIE
                     DAD"
     gene            2297..3928
                     /gene="pruA"
                     /locus_tag="PKH68_07090"
     CDS             2297..3928
                     /gene="pruA"
                     /locus_tag="PKH68_07090"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNX12740.1"
                     /translation="MSKGFYHLPEIKNEPVKTYAPGSTERQSIKLKLAELSKGGLDIP
                     MIIGGKEVRTGNLHDIRPPHDHRQLLGHYHQGDKKHVQLAIDAALTAKPAWEAMNWES
                     RAAIFLKAAELIAGPYRDEFNAATMLGQSKNVYQAEIDCVCELVDFYRFNVRNMCELY
                     AMQPNSSPGIWNRLEWRPLEGFIFALTPFNFTSIAGNLGGAPALMGNTVVWKASRTAV
                     YSASLIMKVLREAGLPDGVINLVFCGGKETADVVFGSRDFAGLHFTGSTAVFNGMWKT
                     ISENISRYRSYPRIVGETGGKDYVFADATANPRQVATALIRGAFEYQGQKCSAASRAY
                     IPTNIWEEVKNYMAEDLKKIKVGDVQDFTNFVNAVIDEESFDKLAKYIDDAAQSPDAE
                     IILGGKCDKSTGYFIHPTVILAKKPDYITMREELFGPVLTVYVYDPQKMEETLDILDN
                     STGYALTGAIFSAERATIEYLTKRLTHTAGNFYINDKPTGAVVDQQPFGGGRGSGTND
                     KAGSIFNLLRWVSPQCIKETFVTPTDYMYPNFRED"
     gene            complement(4211..4720)
                     /locus_tag="PKH68_07095"
     CDS             complement(4211..4720)
                     /locus_tag="PKH68_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNX12741.1"
                     /translation="MKKLDEHFYCRDAVLVARELLGKTLVRVTDDGVASRYRITATEA
                     YLGGDDLACHASKGRTPRTEVMFDSGGKVYVYLIYGMYWMLNIVTGEKEHPQAVLICG
                     LDEVKGSGRVGRLLKMDKSFYGEDLMTSNRIWLEDNIECVNFVTKPRVGVDYAGEWKD
                     KLWRFETVE"
     gene            4876..4946
                     /locus_tag="PKH68_07100"
     tRNA            4876..4946
                     /locus_tag="PKH68_07100"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4908..4910,aa:Gln,seq:ctg)
     gene            5139..5636
                     /locus_tag="PKH68_07105"
     CDS             5139..5636
                     /locus_tag="PKH68_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNX12742.1"
                     /translation="MKILALVRHAKAEQPEPEQTDFDRNLTSRGKRNAAEMASIVKSN
                     GFTPELLITSPAKRALKTASLFADEFAIEKKKILKKDFLYGSFGIPEIKELLHAEAND
                     RDTVFIFGHNPVLSWLSATLSRSFSQGLPTSGVVIIEFDTDSWDKISPSNSKVLLYKY
                     PSEIV"
     gene            5833..>6398
                     /gene="tsaA"
                     /locus_tag="PKH68_07110"
     CDS             5833..>6398
                     /gene="tsaA"
                     /locus_tag="PKH68_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNX12743.1"
                     /translation="MSALKIIARIRTDFPTKFGVPRQSGIVNTLKAMIVFEPEYRNAS
                     ALRGLEEFSHIWLIWGFSEAERDEWSPTVRPPRLGGNRRMGVFATRSPFRPNAIGLSS
                     VKLDDIGKHPEFGTVLYISGADLMDNTPIYDIKPYLPFTDSHPDAFGGFTDTLPEYML
                     EVEFPDNLLNLIPDHMHRGLLEVLAQDPR"
BASE COUNT         1786 a         1457 c         1383 g         1772 t
ORIGIN      
        1 gcatttaccc tgatttcgta cggttgcatg aatttcacca gatttttgac caacgcatgc
       61 acagccgact tggaaactcc gtaagccagt gaaacagaat gagcatattc acccatcagc
      121 gaaccggtaa aaagcacatt acctcccggt tggagggtcg agcaaattcg ctgaagcata
      181 aataccggaa aatgaacatt ggcaaaaaat acccgctccc actcttccag tgtcatttgt
      241 tcaaaagggg ttctgcaggt aagaccagcg ttgaatacga cagaaaaaag atgaatatca
      301 tgtgtattca gatagttttc aatggtatca atagcttgtt tttcagaaat atcagcctgt
      361 aaaacctcca cgctggccgg atataattcc tgaaattctg cctttacttt ggcggcctgc
      421 acttcgtccg acccgtaggt gagtatgagt ttataaccag attgcgccag gatatcggct
      481 acagcgcgcc cgataccttt ggttccgcct gttatcaata tatatttttc catttttttc
      541 agatgagatg tatggtgtta ttgctaatcg atgctatctg aattcatttc aaatattttt
      601 tcttttgggc aataaaagtt tcaaaaaaaa atcagacgca gtgacaaagt taattaatat
      661 ttatgatgaa atacggtttt tttttgaaaa actaccattt cattcttctc aattgtcacg
      721 caaagatgaa ttataacatg ttgaaagtgg gtgaaaaatc aggtatcagc tctcataatt
      781 gtgatattct gactactttt gcacaacaga atgaaaaatt tgtttctttt aattttcagg
      841 tttcaatggt tttaaaggaa gcaacattga acaaattacg ggcaaaataa attcagaaaa
      901 aaaatggcag aaaaaaagaa gattttcggc tttactccca atgtgtttta taccggtata
      961 gtcagttttc tgactgacac atcggtaaaa atggtttact ccgttatgcc gatgttcctg
     1021 atgtccatcg gagcatccaa aaccagtctg tcgctcatcg aaggcattgc cgaaagtacc
     1081 gcctcgctta ttaaggcatt ttcgggttac tggagcgacc ggattggaaa aaacaaaccc
     1141 ttcatgttga ttggctacgg tttgtcggcg gtaatcatgc ccgtttatgc actggtggtc
     1201 agtccgttgc aggttctgat tttgcgattt atcgaacggg tgggcaaagg cattcgcaca
     1261 gcaccccgcg acagtctggt ggccggttcg gtcaacggca atgaggtggg aaaaagtttc
     1321 ggacttcaca aagccatgga caacagtggc gccattctgg gccctttact ggcttttttg
     1381 ttgcttctgt ttcaccccgg cgatttccgc cttatctttt tattagccgg tattcccggg
     1441 ttactgggaa tatttgtgct ctattttggc attcatgaag ccaaaaagca taaagaaagt
     1501 cttttgaaaa aattccgttt ctctgattat cccaaacgat tttatctttt tctggccatt
     1561 atattcgtct tcacattggg gaattcgacc gatgccctgt tgatggtcaa ggccaacgag
     1621 gtgggcatta aagtgacgtt cattccgctg gtatatctta tttccagcat tgtgtcggtg
     1681 tttctcgcca taccattcgg tcagttgtcc gaccgcatcg gacgcgaaaa gctgctgatc
     1741 gccggatttt tcatctatgc gatggtttat ttcggctttg gcataaccgc caatgtgtat
     1801 gtaatcgttg cacttttcgc tttttacggg atctattctg cccttaccga cggccagcag
     1861 aagtcaatga tctctgatct ggtagatgaa aacaaaaaag gaaccggaat gggcatttac
     1921 aatgctttac tgggcatcac gctcttgccc gcaagtctga ttgccggttt gctgtacgat
     1981 aaagtgaatt caaagatacc cttttacttt ggcggttcca tggcgttgct ggctatcgtg
     2041 ttaatggtaa tttatttagc cacaaaacca aaaattgaag acgctgatta aacattcgta
     2101 aaatggagtt attcatcaat tggggtgaat tgcaattcat acaatgccgg gatttagttt
     2161 ccggcatttt tttgtctgaa attgatttaa ttttcaggaa taagcgtttt atttcaaaag
     2221 aaattttata gatttgtgtc attataaaaa ttcagaatat attcacattt tatcaacccc
     2281 tataaatcaa acaaccatgt cgaaaggatt ttatcacctg cccgaaatca agaatgaacc
     2341 tgtaaaaacc tacgcaccgg gttccaccga aagacaatcc atcaagctca aactggccga
     2401 actctccaaa ggcgggctgg acattcccat gattataggg ggcaaagaag ttcgcaccgg
     2461 caatctgcat gacatccgtc cgccgcatga tcaccggcag ttgctcgggc attaccatca
     2521 gggcgataag aagcatgtgc agctggctat tgatgcagcg ctgacagcaa aaccagcctg
     2581 ggaagcgatg aattgggaaa gtcgggctgc catttttctg aaggcagcag aactgattgc
     2641 cggaccctat cgcgacgaat ttaatgccgc caccatgctc ggtcaatcca aaaacgtata
     2701 tcaggccgaa atagactgcg tatgtgaact ggtggatttt tatcgtttca atgtcagaaa
     2761 tatgtgtgag ttgtatgcca tgcagcccaa ttcctcaccc ggcatctgga atcgtctgga
     2821 atggcgtccg ctggaagggt ttattttcgc attgactccg tttaatttca cttccatagc
     2881 cggtaatttg ggcggtgctc ctgctctgat gggcaatacc gtggtatgga aagcatcccg
     2941 gactgccgtt tactccgctt cgctcattat gaaggtgctc agggaagccg gactacccga
     3001 cggggtcatc aacctggtgt tctgcggggg taaggaaaca gccgatgttg ttttcggcag
     3061 tcgtgatttt gcaggattac atttcaccgg ttccacagcg gtattcaatg gcatgtggaa
     3121 aaccatttcg gaaaacatat cgcgctatcg cagctatccg cgcattgtgg gcgaaacggg
     3181 cggaaaagac tatgtttttg ccgatgccac agctaatccc cgacaggttg ccacagccct
     3241 catccgcggc gcattcgaat atcagggtca aaaatgttcg gctgcttcac gtgcctatat
     3301 tccaacgaat atatgggaag aggtgaaaaa ttatatggcc gaagatttga agaaaatcaa
     3361 agtgggtgat gtgcaggatt tcaccaattt tgtgaatgct gtgatagacg aagaatcgtt
     3421 cgacaaactg gctaaataca ttgatgatgc agcccaatcg ccggatgctg aaatcatact
     3481 tggtggaaaa tgcgataaat ccacaggata tttcatccat cccacggtaa ttctggccaa
     3541 aaagcccgat tacatcacca tgcgcgaaga gcttttcgga ccggttctca ccgtctatgt
     3601 gtacgatccg cagaaaatgg aagagacact ggatatcctg gacaattcga ccggctatgc
     3661 gctcacgggt gctatatttt cggccgaaag agcgaccatc gaatacctga cgaaacgcct
     3721 gacacacaca gccggcaatt tttacatcaa cgacaaacca acgggagcag tagttgatca
     3781 gcagcccttc ggtggcggtc ggggttcggg caccaacgac aaggccggct ccatcttcaa
     3841 tctgcttcgc tgggtttcgc cacagtgcat caaagaaacc tttgttacac caacagatta
     3901 tatgtatcca aatttcagag aagactaaac gtttgacgtt caacgttcaa cgtttgaggt
     3961 tagtacgggc caaaatagcg ttgaactgtt gaagcgttga attgttgaag cgttgaactg
     4021 ttgaagcgtt gaactgttga attgttgaac tgttgaattg ttgaagcgtt gaattgttga
     4081 attgttgaac tatagaattg tgtaatcgtg taacactcaa acaagaaaca agaaacaaga
     4141 aacacggaac gagaaacatt gaacgttaaa cacgaaacat taaacattga acattaaaca
     4201 ttaaacgtga tcattccacc gtttcaaacc gccacaattt atctttccat tctccggcat
     4261 aatccactcc tacgcggggt ttggtcacga aatttacaca ttcaatatta tcttcgagcc
     4321 agatacggtt agaggtcatt aaatcttcac cgtaaaaact cttgtccatt ttcagcaacc
     4381 tccccactct acccgaacct ttcacctcat ccagtccgca aataagcacc gcctgtgggt
     4441 gttccttttc gccggtcacg atattcagca tccagtacat gccgtagatg aggtaaacat
     4501 acactttccc gccgctgtcg aacatcactt cggtgcgcgg tgtacgtccc ttgctggcat
     4561 ggcaggccag gtcatcacct cccaggtagg cttcggtggc agtaatccgg tatcgggatg
     4621 caactccatc atcggtaacc cgtaccagcg ttttgccaag taactcccgg gctacaagca
     4681 cggcatcacg acaataaaaa tgttcatcca gttttttcat acctttttgt ttcattttca
     4741 aacaaaagta ataacatgcc tcaaaattga cggctttatt tcagattttt agtgataaat
     4801 tagcattcac tctaaaaatc ttatcccgct acattttgca atataaaaat tttatgtaat
     4861 tttgcagtcc gaaactgtcc catggtgtaa tggtagcata ccagtctctg aatctgtttg
     4921 tccaagttcg aatcttggtg ggacaacaca gaatagcaat acaaatactt gtgttgctat
     4981 tttcatttta aatggtaaga tttcgggtaa gaacaatatt tcagtacaca acaaataccg
     5041 gagataaact gttattaatt aagcaggtag aaaatacata cctctgccac ttacatctta
     5101 aattaatccg gtgtgaaatc cgaaattctt ttctgcccat gaagatatta gcccttgtgc
     5161 gccacgccaa agccgaacaa ccggaaccgg aacaaactga ttttgaccgc aatcttacct
     5221 cgcggggcaa acggaatgct gccgaaatgg cttcaatcgt taaaagtaac ggttttacac
     5281 ccgaattact gatcacaagt ccagctaaac gggcactgaa aacagccagt ctgtttgcgg
     5341 acgaatttgc cattgaaaag aaaaaaatcc tgaaaaagga ctttttgtac ggcagcttcg
     5401 gcattcccga aatcaaggaa cttcttcacg cggaagccaa cgaccgcgat accgtattta
     5461 ttttcggtca caatccggtg ctttcctggc tttcggccac gctgtcgcgc agtttcagtc
     5521 agggtctgcc aacctccggg gttgtaatta ttgagtttga taccgacagc tgggataaaa
     5581 tcagcccatc caacagcaaa gtactgctgt ataaatatcc gtcggaaatt gtatgattat
     5641 ccaatttaag gaagccatcc gggataacat tcctttaata aaatgacatt ttacaacaat
     5701 taaatgatca aaaagctgtt ttgaaaactt caaaagagct ttttttgtgt tttatgaaaa
     5761 gcagatgcta tgctccgcca tatttttgta aatttgtccg gcaaaacgaa accattcacc
     5821 gaacccgatt acatgtcagc acttaaaatc attgcccgca tccgtaccga tttccccacc
     5881 aaatttggcg ttccgcgcca gagtggaata gtgaacacgc tgaaggcgat gatagtattc
     5941 gaaccggagt accgcaatgc ctctgccctg cgcgggctgg aagaattttc acacatctgg
     6001 cttatctggg gattttcgga agccgaacgc gatgaatggt cgccaacggt aagaccaccg
     6061 cggctgggtg gcaaccggcg catgggagtt tttgccaccc gctccccttt ccggccgaat
     6121 gccatcggac tttcatcggt caaactcgat gatatcggga agcatcccga attcggaaca
     6181 gtgctttaca tttcgggagc cgatctgatg gacaatacac ctatctacga catcaaacct
     6241 tatctgccct tcaccgacag tcatcccgat gctttcggcg gatttaccga cacactcccg
     6301 gagtatatgc tggaagtgga atttccggat aatctgctta accttattcc cgatcatatg
     6361 caccggggac ttctggaagt gctggcacaa gatccgcg
//
LOCUS       DAOPZK010000111         6335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_15116_length_6335_cov_28.111306, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000111 DAOPZK010000000
VERSION     DAOPZK010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15116_length_6335_cov_28.111306"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..1221
                     /locus_tag="PKH68_07115"
     CDS             577..1221
                     /locus_tag="PKH68_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HNX12744.1"
                     /translation="MNKLISPSDAASFVKDGMTLMIGGFLGHGTPERIIDEIVASGVK
                     SLTLIVNDTAFPDKGCGKLVTNNQVKKLIVSHIGTNPNTGQKMNSGELEIEFSPQGTL
                     AERIRSGGAGLGGILTPVGIGTIIEEGKQMIQVDGTDFLLEKPLRADIALIYATTADD
                     LGNLIYMGTMRNFNPLMAMAADTVIAEVEELKPAGELSPETIHTAHIFIDYIVH"
     gene            1237..1620
                     /locus_tag="PKH68_07120"
     CDS             1237..1620
                     /locus_tag="PKH68_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNX12745.1"
                     /translation="MEKSMIRLRMSAHDAHYGGNLVDGARMLQLFGDVATELLIRHDG
                     DEGLFVAYDNVEFLNPVFAGDYIEATGEITKTGNSSRKMIFEAKKVIRARTDISASAA
                     DVLEEPVVVCRASGTCVVPKTCQRK"
     gene            1662..2480
                     /locus_tag="PKH68_07125"
     CDS             1662..2480
                     /locus_tag="PKH68_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNX12746.1"
                     /translation="MEKLIITAAICGAEVTKEQNPAVPYTIEEMVREAKSAYEAGAAV
                     VHVHVREDDGTPTQSKERFKLCMDAIKKEIPDVILIPSTGGAVGMTAEERLQPTELFP
                     EMATLDCGTCNFGDEVFENTMPMMRSFGKRMIDNNIKPEYECFEMGHLDTILRMAGKG
                     EVPGFPMQINFVLGVPGCAPATVENLVWLVRNVPAGCTWTATGIGRNAFTLAAPAIVM
                     GGHVRVGFEDNLYISKGVLAKSNGELVQKVVRLATELGREIATPAEARKILNLQ"
     assembly_gap    2483..2582
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2687..3727
                     /locus_tag="PKH68_07130"
     CDS             2687..3727
                     /locus_tag="PKH68_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNX12747.1"
                     /translation="MKKGNRYGTHRVIEPKGVLPQPANKIDNNMDEIYDNEILIDVQT
                     LNIDSASFTDIYARAEGNSEKIAEIMMEIVATQGKHRNPWTGSGGMLLGTVEKIGDAL
                     LGKTDLKVGDKIATLVSLSLTPLRIDKIKEIRHEVDQVDIDGKAILFESGIYAKIPSD
                     MPEKLALSALDVAGAPAQTAKLVKPGDTVLIIGAGGKSGMLCCYEARKRAGITGKVIG
                     ITHSQKSTERLEKLGFCNHVFSADATQPVSVLEKIEELTNGELCDITINNVNIPDTEM
                     TSILCTKDTGIVYFFSMATSFTKAALGAEGVGSDVTMLIGNGYTKGHAEITLQELRES
                     EELRKIFTELYA"
     gene            3739..>4555
                     /locus_tag="PKH68_07135"
     CDS             3739..>4555
                     /locus_tag="PKH68_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNX12748.1"
                     /translation="MAESKRKIFFPEVSDEQWNDWKWQVKNRIETVEELKKHIKLTEE
                     EEVGIRESLKTLRMAITPYYLSLIDTDDPHDPIRKQSIPTFNELYISPDDLQDPLHED
                     SDSPVPGLTHRYPDRVLFLITDMCAMYCRHCTRRRFAGQRDAALPTERIDKAIDYIAR
                     TPQVRDVLLSGGDALLMSNDKLEYILKKLRDIPHVEIIRIGTRTPVVMPQRITDDLVN
                     MIKKYHPVWVNTHFNHPNEITPESTEACGRLADAGIPLGNQSVLLRGINDCSHV"
     assembly_gap    4556..4655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4656..5129
                     /locus_tag="PKH68_07140"
     CDS             <4656..5129
                     /locus_tag="PKH68_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNX12749.1"
                     /translation="PLGNQSVLLRGINDCSHVMKKLVTGLVKIRVRPYYIYICDQSMG
                     ISHFRTPVSKGIEIIENLRGHVSGYAVPTFVVDAPGGGGKIPVMPTYLISQSPSRVVV
                     RNYEGVISTYAQPTDYTDECHCADCSKKQGIAGLLAGEQLSLEPKDFQRHKRNGH"
     gene            5142..6152
                     /locus_tag="PKH68_07145"
     CDS             5142..6152
                     /locus_tag="PKH68_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12750.1"
                     /translation="MPFVNEILAYRSVSIAGLEKNTGKTESLNYVLNRLKTMNTCIAL
                     TSIGVDGEKTDQVTRSPKPEITVYEGMIFVTSEKHYLERKIISEILDVSQERTALGRL
                     ITARAKIQGKVILSGPSGTAEIRKLIENMQQYGVNTVIVDGALSRLSFASPAVTDAMI
                     LATGASLSANIPQLVSKTNFVKQLIELPLVEDEMAKRLQELKQGIWGIDETGRIHDLE
                     ISSVFLLDKTDKDIFRFGNRIFIPGAITDKLIAFLLTQNKKVELIVSDFSRIFCSQNH
                     FSSFLKRGNTLKCILQSKLIAITVNPVSPNGFTLNRDRLLAEMQETLKVPVYDIKSEI
                     CS"
     gene            6143..>6335
                     /locus_tag="PKH68_07150"
     CDS             6143..>6335
                     /locus_tag="PKH68_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12751.1"
                     /translation="MQLRQAIQEISGMKYMTGSLNVLSPIGKNYLYDLPFLTTKDEIE
                     AVLNDTEAVVDLLKENTREN"
BASE COUNT         1939 a         1269 c         1398 g         1529 t
ORIGIN      
        1 tcacgtctaa aaatgaataa ctttgaattt gaaatcgact gacaatatca ttgagctcgt
       61 cttgactttt tttataaaac cataatgtcg cacctacgga gcttacaaac gatataataa
      121 tccattttct accataatat cgcacctctg gtgctaagaa tgaagcctca tttaagcaat
      181 gtcagggtag actattttag agttgctaat taaagcctcg tagaggcgac attataaaat
      241 gtctggacaa cttcgctttt agctattcaa acaacaaatc gtttgttgtc atccgccaca
      301 attatctttc tccccacaga tttgttttat taaaaagaaa cgattacttt tttttaattg
      361 cattacactg actctaaaca ttaaaaagtc agtaatcctg ctttgttacc ccaataaatc
      421 tatagaaata accttaaaac tattaaaatg tcaatcaaaa ttaatcaaaa tgtaagttgt
      481 atcctgaatt tttcactaaa aaaattgttt atctgaaaaa taaatttata tatttgtagc
      541 atctttatgc aaaaaaaata attaaatcca tctgtgatga ataaattaat ttcgccttcc
      601 gacgctgctt catttgtaaa agatggcatg actttaatga tcgggggatt tctgggacac
      661 ggaacacccg aacgaattat cgacgaaata gtggcaagcg gagtgaagtc attaacatta
      721 attgtcaatg atacagcatt tcccgataaa ggatgcggta aactggttac aaataatcag
      781 gttaaaaaac tgattgtatc acacattggc accaatccca atacgggaca aaaaatgaac
      841 agcggcgaac tggaaattga attttcgcct cagggtacgc tggccgagcg gatcagaagc
      901 ggtggtgccg gactgggagg aattttaacc ccggtgggca tcggaacaat catcgaagaa
      961 ggcaaacaaa tgatacaggt ggatggtacc gattttctcc ttgagaaacc gctgcgggca
     1021 gatatcgcgc tgatttacgc cactacggcg gatgatttgg gcaatctgat atacatggga
     1081 accatgagaa actttaatcc gctgatggct atggctgccg atacggttat tgccgaagtg
     1141 gaagaactga aacctgccgg agaattaagc ccggaaacca ttcacaccgc gcatattttt
     1201 atagattata ttgtacatta aaatacttac acagaaatgg aaaaatcaat gatacggctt
     1261 cgcatgagtg ctcacgatgc acattacggc ggaaatctgg tcgacggtgc caggatgctc
     1321 caacttttcg gcgatgtagc cactgaactg ctgatacggc atgatggcga tgaaggttta
     1381 tttgttgcct atgataatgt ggaattctta aatccggttt ttgccggaga ttatattgaa
     1441 gctaccggag aaattacaaa aaccggaaac tcctcgcgta aaatgatttt cgaagcaaaa
     1501 aaagtaattc gggcacgtac tgatatttcg gcttcggccg cagatgtgct cgaagagccg
     1561 gttgtggtct gccgcgccag cggaacttgt gttgtaccta aaacctgcca gcggaaatag
     1621 aatgtatttc tgaataaatt caacaccaat acttacataa aatggaaaaa ctaatcataa
     1681 cggctgccat ttgcggtgcc gaagttacca aagaacaaaa tccggctgtt ccttacacga
     1741 ttgaggaaat ggttcgtgaa gcaaaatcgg cttacgaagc gggcgctgcc gttgtccatg
     1801 ttcatgtgcg ggaagatgac ggtactccca cacaaagcaa ggaacgtttc aaactgtgta
     1861 tggatgctat taaaaaagaa attcccgatg tgatccttat cccttccaca ggcggtgcag
     1921 taggtatgac ggccgaagag cggttgcagc ctaccgaatt gtttcccgaa atggccacgc
     1981 tcgattgcgg aacctgtaac tttggcgatg aggttttcga aaataccatg cccatgatgc
     2041 gctcattcgg caaaagaatg attgataata atattaaacc ggaatacgaa tgttttgaaa
     2101 tgggacatct ggataccatt cttcgtatgg ctggcaaagg cgaggttccg ggtttcccga
     2161 tgcagattaa ctttgtactg ggcgttccgg gatgcgcacc tgcaactgtg gagaatctgg
     2221 tatggctcgt gcgcaatgtt ccagccggat gtacctggac agcaaccgga ataggcagaa
     2281 atgcattcac actggcagct ccggctatag tcatgggcgg ccatgtccgc gttggctttg
     2341 aagataatct gtacatttcc aaaggcgttt tagctaagtc gaacggagaa ctggtgcaaa
     2401 aagtggtgag actcgccact gaactgggac gtgaaatagc aacgcctgcc gaagcaagga
     2461 aaattctaaa tctacaataa tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nngacgtgaa atagcaacgc ctgccgaagc aaggaaaatt ctaaatctac aataatccta
     2641 tcttataaaa ataccattaa aaataccatt aaaaatacca tcaacaatga aaaaaggaaa
     2701 cagatacgga actcaccgcg taattgagcc caaaggggta ttgccacaac ccgccaacaa
     2761 aatagataac aacatggacg aaatctatga caatgaaatt ctcattgatg ttcagactct
     2821 caatatagat tcagcgtcct ttaccgatat ttacgccaga gcggaaggaa attccgaaaa
     2881 aatagctgaa atcatgatgg aaattgtcgc cactcagggc aaacaccgga atccgtggac
     2941 gggttcgggc ggtatgttgc tgggaacggt ggaaaaaata ggggacgcgc tgcttggaaa
     3001 aacagacctg aaagtgggtg ataaaatagc cacactcgtt tcgttatcgc ttaccccgct
     3061 gcgaattgac aaaataaaag aaatccgtca cgaagtggat caggtggata tcgacggaaa
     3121 agctatcctg ttcgaaagcg gtatctatgc caaaatacct tcagatatgc ccgaaaagct
     3181 ggccttatcg gctcttgatg ttgccggtgc acccgctcaa acggccaaac tggtgaaacc
     3241 gggtgacact gtgctcataa tcggtgcagg aggtaaatcg ggaatgcttt gctgctacga
     3301 agcccggaaa cgtgccggaa ttaccggaaa agtaatcggg ataacacatt cacagaaaag
     3361 caccgaaaga ctggagaaac tcggcttttg caatcatgtt ttttcggcgg acgcaacgca
     3421 gcctgtttcg gttctcgaaa agattgaaga acttacgaac ggagaattgt gcgatataac
     3481 cattaataat gtcaatattc ctgacaccga aatgacaagt attctttgca caaaagatac
     3541 gggaatagtt tatttctttt ccatggctac cagttttaca aaagcagcac tcggagccga
     3601 aggggttggc agcgatgtaa ccatgcttat cggaaacggc tatacaaagg gccacgctga
     3661 aataaccctg caggaacttc gcgaatcgga agaactcaga aagattttca ctgaactgta
     3721 tgcttaatac tttattcaat ggctgaaagt aaaagaaaaa tattcttccc ggaagtttct
     3781 gacgaacaat ggaacgattg gaaatggcag gttaaaaacc gcattgaaac ggttgaggaa
     3841 ttaaaaaaac acatcaaact cactgaagaa gaggaggtgg gcatacggga atcgctcaag
     3901 acactgcgca tggccatcac tccgtattat ctgagtttga tcgacacgga cgatccgcac
     3961 gatccgatac gtaagcaatc aattccaaca tttaacgaat tatatatttc tcctgacgat
     4021 ttgcaggacc ctttgcacga agacagcgat tcaccggttc ccggactgac gcaccgttat
     4081 cctgaccgcg tgctgtttct gattaccgat atgtgtgcca tgtattgccg tcattgtaca
     4141 cggcgcaggt ttgcaggtca aagagacgcg gcattgccta cagaacgtat agataaagcg
     4201 atagattata tagcccggac tccacaggtc agggatgtgc ttctgtcggg cggagatgcc
     4261 ttgctgatga gcaatgacaa acttgaatac attctgaaaa aactccgcga catacctcat
     4321 gttgaaatca tccgcatcgg tacacgtact ccggtggtaa tgcctcagcg tattacggat
     4381 gacctggtga atatgattaa gaagtatcat ccggtgtggg taaatacaca ttttaatcat
     4441 ccgaacgaaa ttacgccgga atcaacagag gcctgtggca gacttgccga cgcaggtatc
     4501 ccgctgggta atcagtcggt gcttcttcgc ggcatcaacg attgcagtca tgtgannnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgct gggtaatcag tcggtgcttc
     4681 ttcgcggcat caacgattgc agtcatgtga tgaaaaaact ggtgaccgga ttggtgaaaa
     4741 taagggtgag accatactat atctacattt gcgatcagtc aatgggaatt agtcacttcc
     4801 gcactccggt atcgaaaggc attgaaataa tcgaaaatct gcgggggcat gtttccggat
     4861 atgctgttcc gactttcgtg gtggatgctc ccggcggagg aggaaaaatt ccggtcatgc
     4921 caacctatct gatttcacaa tctccgagca gagtggtggt ccggaattac gaaggtgtga
     4981 taagcaccta cgcgcaacct accgactata ccgatgaatg tcattgtgct gattgcagca
     5041 aaaaacaggg catcgccgga ttgctggcag gtgaacagtt gtcgctcgaa ccgaaagatt
     5101 ttcaaagaca caaacgaaac ggacattaaa ataaattccg gatgccattt gtcaatgaaa
     5161 tattagcgta ccggagtgtt tccattgcag gacttgaaaa aaacaccgga aaaactgaaa
     5221 gtttgaacta tgtcctgaac cgtctgaaaa cgatgaatac atgcatagcg ctgacctcca
     5281 tcggagttga cggagaaaaa acagaccagg ttacaaggtc gccgaaaccc gagattacgg
     5341 tttatgaagg gatgattttt gttacatcag aaaaacatta cctcgaaaga aaaataattt
     5401 cggaaattct cgatgtaagt caggaacgca cagcgctggg aagactgatt actgctcgtg
     5461 ctaaaattca gggaaaggta attctttcgg gaccttcagg gacagctgaa atcaggaaac
     5521 tcattgaaaa tatgcaacaa tacggggtta atacggtgat tgtggacgga gcattatcgc
     5581 gcctcagctt tgcatcgcct gctgttaccg atgccatgat actggctacg ggagcatctt
     5641 tgtcggcaaa tattcctcag ctggttagca aaacaaattt tgtcaaacag ctgattgaat
     5701 tgccactcgt tgaagatgaa atggcaaaac gattgcagga gctaaaacag ggtatctggg
     5761 gaattgatga aaccggaagg attcacgatt tggaaatttc atccgttttc ctcctcgaca
     5821 aaaccgataa ggatattttc agattcggaa accggatatt tataccggga gccatcaccg
     5881 acaaactgat tgcatttctg ctaacacaga ataagaaagt tgaattgatt gtcagtgatt
     5941 tttccagaat attctgttcg caaaatcact tcagttcttt cctgaaaaga ggaaatacac
     6001 tgaaatgtat cctgcaaagc aaattaatag cgataaccgt gaatccggta tcaccgaatg
     6061 gtttcacact caacagggac agactgctgg cagaaatgca agaaacattg aaagtaccgg
     6121 tttacgacat aaaaagtgaa atatgcagtt aaggcaagcc atacaggaaa tcagcggaat
     6181 gaaatatatg accggttcgc tgaatgttct ttccccgata gggaaaaact atctttatga
     6241 cttacccttt ctgacaacaa aggatgaaat agaagctgta ctgaatgata cagaagctgt
     6301 ggttgatctt ttgaaggaaa acacaagaga gaata
//
LOCUS       DAOPZK010000112         6333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_15123_length_6333_cov_23.985505, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000112 DAOPZK010000000
VERSION     DAOPZK010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15123_length_6333_cov_23.985505"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1130)
                     /gene="asnA"
                     /locus_tag="PKH68_07155"
     CDS             complement(93..1130)
                     /gene="asnA"
                     /locus_tag="PKH68_07155"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNX12752.1"
                     /translation="MSYYIKPKNYNPALDLQQTELGIQKIKDFFQANLSAELRLRRVT
                     APLFVLRGTGLNDDLNGVERAVNFPIKDMQDARAEVVHSLAKWKRVTLADYNIENGYG
                     IYTDMNAIRADEELGNLHSLYVDQWDWERVMEEGERNIGFLKSIVKKIYAAFLRTEYL
                     VSESFPEIRPCLPEEIVFIHSEELLQLYPTLTPKEREYEIAKKHKAVFIIGIGGDLSD
                     GQKHDGRAPDYDDWTTSGENGLPGLNGDIILWNPVLEVPFEVSSMGIRVDKKALIEQL
                     RISGKEDRLELYFHKRLMNDTLPLSIGGGIGQSRLCMFFLRKAHIGEIQASIWPEEVI
                     AECKKENIVLI"
     gene            complement(1332..1838)
                     /locus_tag="PKH68_07160"
     CDS             complement(1332..1838)
                     /locus_tag="PKH68_07160"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNX12753.1"
                     /translation="MSKVSIIAAVADNLAIGKDNKLPWHLPADLKHFKELTTGHAVVM
                     GKRTFESLPNGPLPNRKNIVLTSIPEGNFDKYYEATSIKDAIELCENEDQVFIIGGSS
                     IFAQSIKTVDTMYITWVHGKFEADTFFPKFNLREWNEVSREDHETDERNPYSYTFCKY
                     ERKEKKGK"
     gene            complement(1970..2764)
                     /locus_tag="PKH68_07165"
     CDS             complement(1970..2764)
                     /locus_tag="PKH68_07165"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNX12754.1"
                     /translation="MKQYLDLLNRVLAEGVKKEDRTGTGTLSIFGHQMRFNLEEGFPC
                     LTTKKLHLKSIIYELLWFLNGDTNAKFLQDNGVKIWNEWADENGDLGHIYGYQWRSWP
                     DYQGGSIDQITQAVETIRNNPDSRRIIVSAWNVADLPNMNLPPCHAFFQFYVAEGKLS
                     LQLYQRSADIFLGVPFNIASYALLLQMMAQVTGLKAGDFIHTLGDAHIYLNHLEQVNL
                     QLSREPRSLPQMLINPAIKDIFGFQYSDFELVNYDPHPHIKGIVAV"
     gene            complement(2927..4921)
                     /locus_tag="PKH68_07170"
     CDS             complement(2927..4921)
                     /locus_tag="PKH68_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX12755.1"
                     /translation="MLKSKSFVLLILLAVLTLQSCGIKARLAKADKRYALGEYAVAGD
                     LYKSVYSRIPSKNKPLRAEIAFKQGECYRLINHYRAEQVYQNAVRNKSTNDTVYLRLA
                     QMQQRNGKYADAAKSYTSYLEKHPEDELALNGLQATALAAEMKASPTRYLVKKSKEFN
                     ISRSSTFSPLFSGSEGDVLIFTSNRKFGKKNVVRKNNGVSGQPNNHLFMTRKNNKGKW
                     EAIEPLEGDVTSLNDEGIASITSDGRSMYFTRSLLADDKGGGTLIVLSNRAGGTWSAP
                     QKVTIFKDSTISVGHPAITPDGTTMYFVSDSKEGLGGKDIWRATYEGGEWKYIENLGK
                     DINTPDDEMFPTVREDGTLYYSSKGKPGMGGLDIFKATPLKEGGWEVKNMGVPVNSPA
                     DDFGMAFAGKTESGYFSSNRNESRGYDALWSFELPALEYVLQGKITDRVGETVADAKI
                     RIIGNNGENARIQAKGDGTYSFKMTKGVDYVMLASARGFLNQKNEISTRNASDRQSES
                     FKVDFQLTPLFKPVQMDNIFYEFAKWNLTPQSETGLQQLVKLLNDNPNITIEMSAHTD
                     YVGNNESNRILSERRAQSVVDYLIKAGIDKKRLTAVGYGEEQPFTVDINTAKKYNFLK
                     ENTVLTEAFIQTLTPVQQEIANQINRRTEFRVLKTNYNLY"
     gene            complement(5003..6250)
                     /locus_tag="PKH68_07175"
     CDS             complement(5003..6250)
                     /locus_tag="PKH68_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNX12756.1"
                     /translation="MAIIKPFKGIRPPKDLVEKVASRPYDVLSSEEARQEAAGNPYSL
                     YHIIKPEIDFAPGTDEHDEKVYLKAAENFAMFREKGWLVQDETEKYYVYAQTMNGRTQ
                     YGLVVCASVEDYMTGNIKKHELTRRDKEEDRMKHVRVNNANVEPVFFAYPHRDDLDNI
                     VAGIIKHEPEYDFTAELDGFRHQFWVIEDATINQQITAIFAQIPTMYIADGHHRSAAA
                     ALVGNEKKLQNPNHRGDEEYNYFMAVCFPDNQLNIIDYNRVVKDLNGLTEEEFLNKLK
                     ENFIVEEKGTEIYKPDSLHNFSLYLDNNWYSLTAKPGTYNDHDPIGVLDVTISSNLIL
                     DEILGLKDLRSDKRIDFIGGIRGLGELKKRVDSGEMKVALALYPVSMKQLIDIADTGN
                     IMPPKTTWFEPKLRSGLVIHELV"
BASE COUNT         1524 a         1413 c         1398 g         1998 t
ORIGIN      
        1 ctataaatac tcgccaactc cccctttaga gcctgtccct gcctgtcccg atttatcggg
       61 aaatttgttt cgggagggct ggggggcatg tatcagatca acacaatatt ttcttttttg
      121 cattcggcta tcacttcttc gggccagatg cttgcctgta tttcgccgat gtgggctttg
      181 cgcaggaaaa acatacacag tctcgattgt ccgatgccac cgccgatgga caatggaagt
      241 gtgtcgttca tcagcctttt gtgaaaatac aattccagac ggtcttcttt gcccgatatt
      301 ctaagctgct ctatcaatgc ttttttatcc acacgaattc ccatggatga aacttcaaat
      361 ggcacttcca gcaccggatt ccataggatg atatcgccgt tcagaccggg taaaccgttt
      421 tcaccggatg tggtccagtc atcgtagtcg ggtgcgcgtc cgtcgtgctt ttgtccgtcg
      481 ctcagatcac cgccaattcc gataatgaat acagctttgt gttttttagc aatttcatat
      541 tctctttctt tgggggtgag ggtggggtag agttgaagca gttcttcgga atgaatgaaa
      601 acaatttcct cgggcagaca gggtctgatt tcggggaaac tctcactcac caggtattcg
      661 gtgcgcagaa aagccgcata tatttttttt acaatggatt tcaaaaagcc gatatttctt
      721 tccccttcct ccattacccg ttcccaatcc cactgatcca cgtacagcga gtgcaggttg
      781 cccagttctt cgtcggcacg aatggcgttc atgtcggtat aaattccata accattctcg
      841 atgttgtaat cggcaagggt tacccgtttc cactttgcca gcgaatgtac cacttctgcc
      901 cgtgcatcct gcatgtcttt gatgggaaaa ttcacggccc gttccacacc gttcaggtcg
      961 tcattgagac cggttccgcg caatacaaaa agcggagcag taacccgcct caaccttaat
     1021 tcggcggaga gattagcctg aaaaaagtct tttattttct gaatacccag ctcggtctgc
     1081 tgcagatcga gagcgggatt gtaatttttc ggtttaatgt aataactcat aactgattat
     1141 ttgtgatttg ttttcaattc atctggcaaa aatactgtat ttattttaat tgacaaacaa
     1201 aatgtatggt aaaaatgaaa aatgctgtca tattcacatt gtttgtgctt aatctgtgtg
     1261 ttctataaaa caaaacagtt tctgccgggc agcagaaact gattattggt agagtgtttt
     1321 atgcagtggg tttattttcc ctttttttct tttctttcgt acttgcagaa agtgtaggaa
     1381 tatggattgc gttcatcggt ttcgtggtct tcacgactca cttcgttcca ttctctcaga
     1441 ttgaatttag ggaaaaaggt gtcagcttca aatttcccgt gaacccaggt gatatacatc
     1501 gtatcaaccg ttttaattga ctgcgcaaag atggatgacc ctccgataat aaatacctga
     1561 tcttcgttct cgcaaagctc tatggcatct ttgatagaag ttgcttcgta gtatttatca
     1621 aaatttcctt cgggaatgga ggttagtacg atatttttcc ggtttggaag gggtccgttc
     1681 ggcaaacttt cgaaagtgcg ttttcccatc acaacggcat gtcctgtagt caactctttg
     1741 aaatgtttca ggtctgcagg cagatgccag ggtaatttgt tgtctttacc aattgctaaa
     1801 ttgtcagcaa cggcagcaat aattgaaact tttgacatga tatttaacta tttaagtact
     1861 gtttaatttg ctcttttgcg aaaaacagtt gacaaagtta caataaaaaa tgaataaaca
     1921 atcgggttta ttcatttctt agtattcaat acgtttgcta atataaattt cagacagcaa
     1981 ctataccttt aatgtgtgga tgtggatcgt aatttacaag ctcaaaatca gagtattgga
     2041 atccaaatat atcttttatt gccggattaa ttaacatttg aggcagtgat cttggttcgc
     2101 gcgaaagttg aaggttgacc tgttccaggt gattgagata aatgtgagcg tcgcccaggg
     2161 tatgaataaa atcgcctgct ttaagaccgg tcacctgagc catcatctga agcagcaggg
     2221 cgtaagaagc gatattgaaa ggtactccga gaaaaatatc ggcgctccgc tgataaagtt
     2281 gtaaactcaa ctttccttcg gccacataaa attgaaaaaa tgcatgacag ggtggaagat
     2341 tcatattggg tagatcggct acattccagg cactcactat gatgcggcgt gaatcagggt
     2401 tgttcctgat ggtttccaca gcctgcgtta tctgatcgat ggaaccaccc tgataatcgg
     2461 gccacgaacg ccactgatag ccatatatat gtccgagatc gccattctca tccgcccatt
     2521 cgttccagat ttttactccg ttatcctgca gaaattttgc attggtatca ccgttaagaa
     2581 accacagaag ttcataaatg atggatttca aatgaagttt cttggtggta agacagggaa
     2641 atccttcttc cagattgaaa cgcatctgat gtccgaatat gctgagggtg cctgttccgg
     2701 tgcggtcttc cttttttacc ccttcggcca gaacccggtt aagtaagtct aagtattgtt
     2761 tcatcagttt ataatttata aatcgacccc cctaatccgt cagctgacgg aggaatattg
     2821 agcgtctatc atttgttgtg agttttttaa tcatgcccgt atgtgttaaa aaatattcta
     2881 cctggacatg tgtgtatcat gcggattttt ttcttagcgt gtgctgctaa tacagattgt
     2941 aatttgtttt caataccctg aattcagtcc ggcggttaat ctgattggct atttcctgct
     3001 gcacaggagt taatgtctgg ataaaggctt cggtcagcac ggtgttttct ttcaggaaat
     3061 tgtatttctt tgcagtgttt atatccactg taaatggctg ttcttcacca taacccacgg
     3121 cagtcagcct ttttttgtct attccggctt ttatcagata gtcaaccacc gattgtgccc
     3181 tgcgttcgga cagtatccgg ttggattcgt tattgcctac gtagtcggtg tgtgcactca
     3241 tctcgatggt gatattcgga ttgtcgttga gcagttttac cagttgctgc agtccggttt
     3301 ccgattgtgg ggtgagattc catttggcaa attcgtagaa aatattatcc atctgaaccg
     3361 gtttaaataa cggtgtcagc tgaaaatcaa ccttgaaact ctcgctctgg cggtcgctgg
     3421 cgtttcgggt ggatatttca ttcttttgat tcaaaaaacc acgggcagaa gcaagcatca
     3481 catagtccac tccttttgtc atcttaaagc tgtaagttcc gtcacctttc gcctggattc
     3541 gtgcattttc accgttattt cctataatac ggattttggc atcggctaca gtttctccca
     3601 cccggtcggt gattttaccc tgcagtacat attctaatgc aggtaattca aaactccaga
     3661 gcgcatcgta accccggctt tcattccggt tagaggaaaa atatccgctt tcggtttttc
     3721 cggcaaaagc catcccgaaa tcatcggccg gtgaattgac gggaactccc atgtttttaa
     3781 cttcccagcc tccctctttc aatggtgtgg ctttaaatat atccagtccg cccatgccgg
     3841 gttttccttt ggaagaataa tacagcgtgc catcttcccg caccgtcgga aacatttcat
     3901 cgtcgggtgt attgatatct tttcccaggt tttcaatgta tttccattct ccgccttcgt
     3961 aagttgctct ccatatatct ttgccgccca gtccttcttt tgaatcggat acaaaataca
     4021 tcgttgtccc gtcgggtgtg atagccggat gacccaccga aatggtgctg tctttgaaaa
     4081 tagtgacttt ctgaggtgca ctccacgttc ctcccgcccg gttggatagt actatgagcg
     4141 tgccgccgcc tttatcatcg gccagcagtg atcgggtgaa atacatggag cggccatcgg
     4201 aagtaatgga tgcgatacct tcgtcgttga gcgaagttac atcgccctcg agtggctcga
     4261 tagcctccca ttttcctttg ttatttttgc gggtcatgaa aagatggttg ttgggctgcc
     4321 cgctcacgcc gttgtttttg cgaaccacat ttttcttccc aaacttacgg ttggaggtga
     4381 atatcagcac atcgccctcg gaacccgaaa aaagcggact gaaggtggat gaacgactga
     4441 tgttgaactc tttcgatttt ttcaccaggt agcgggtggg actggctttc atctctgcag
     4501 ccagcgccgt agcttgcagt ccgttgagag ccagttcgtc ctccggatgt ttttccagat
     4561 aggaagtgta gctttttgcg gcatcggcgt attttccgtt tcgttgctgc atctgtgcca
     4621 ggcggaggta cacggtgtcg ttagttgatt tattccgcac ggcattctga taaacctgtt
     4681 cggcccggta atggttgata agccggtaac attcgccctg tttgaaggca atttcagccc
     4741 gcaggggctt gtttttcgag ggaatgcggg agtaaacact tttgtacaaa tcaccggcta
     4801 cagcatattc tcccaaagca tagcgtttat ccgccttagc aagccgggct ttgatgccgc
     4861 aggactgcaa ggtcaggact gccagcagaa taagcaaaac gaatgatttg gactttagca
     4921 tataaaaatt gaagcctttg aggaattatt cttttattga ttaacttttt tgacgggctt
     4981 ttattgcccg atagcttctt ttttacacca attcatgaat taccagaccg gaacggagtt
     5041 tcggttcgaa ccaggtggtt ttcggaggca taatgttacc ggtatcggca atgtcaatca
     5101 gctgtttcat cgaaacggga tacaacgcca gcgccacttt catttcaccg ctgtcaactc
     5161 tttttttcag ttcacccagt ccgcgtatgc cgccgataaa gtcaatgcgc ttgtcgctgc
     5221 gaaggtcttt gagccccaga atttcatcca gaataagatt ggaggaaata gtgacatcaa
     5281 gcacaccaat cggatcatgg tcgttgtacg ttcccggttt ggcggtgagc gaataccagt
     5341 tgttgtccag atatagcgaa aaattatgca ggctgtccgg tttgtagatt tctgttcctt
     5401 tttcttcgac aataaaattt tctttcagtt tattaagaaa ttcttcttct gtcaatccgt
     5461 tcaggtcctt taccacccgg ttgtagtcaa tgatattcag ttgattgtcg gggaagcaaa
     5521 cagccatgaa gtagttgtat tcttcatcgc cacggtgatt tggattctga agtttctttt
     5581 catttcccac cagtgcggca gcagcgctgc ggtgatgtcc gtcggcaatg tacatggtag
     5641 gtatctgtgc aaaaattgcg gtgatttgct gattgatggt ggcatcttca attacccaga
     5701 actgatgtct gaatccatcc agttcggctg tgaaatcata ttccggttcg tgtttgataa
     5761 ttccggctac aatattatcg agatcatcgc gatgtggata agcaaaaaat accggctcta
     5821 cgttggcatt attcacccgc acgtgtttca tgcggtcttc ttccttgtcg cggcgggtaa
     5881 gctcgtgttt tttaatgtta ccggtcatgt aatcctccac actggcacaa accaccagtc
     5941 cgtactgagt gcgtccgttc atggtctggg catatacgta atacttctct gtttcatcct
     6001 gaacgagcca gcccttttca cggaacatgg caaaattttc ggcagctttc agatacactt
     6061 tctcgtcgtg ctcgtctgtg ccgggcgcaa aatcaatttc cggcttgata atgtgataaa
     6121 gcgaatatgg atttccggct gcttcctgtc ttgcttcttc cgaactaagt acgtcatacg
     6181 gacgcgatgc cactttttca actaaatcct taggaggacg aatgcctttg aatggtttga
     6241 taattgccat attttctaat tttgtatttt taatgataag tgataagttt tcttggcaca
     6301 aatttacaaa aaaggttttg agtttctatc ttt
//
LOCUS       DAOPZK010000113         6291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_15283_length_6291_cov_20.875882, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000113 DAOPZK010000000
VERSION     DAOPZK010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15283_length_6291_cov_20.875882"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3180
                     /locus_tag="PKH68_07180"
     CDS             <1..3180
                     /locus_tag="PKH68_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX12757.1"
                     /translation="MAQESRRVTGTVIDGNDNTAMIGVNVIEKGTGNGTVTKADGSFA
                     INLTSKNPVLIFSSLGYTSVEEVVGNRTSFKIVLNEDRKTLGEVVVVGYGTMKKSDLS
                     GAAVTVGEDKIKGSIITNLDQALQGRVAGVNSVMTSGAPGSSVSIRVRGQSTINSNAE
                     PLYVIDGVIVQGGGNSGASFGLGDALGNGSTSTISPLSTINPSDIVSMEILKDASATA
                     IYGAQGSNGVVLITTKKGKAGEAKFSYEGLFGVQRQAARIDMMNLREFAEYSNSVSQE
                     SNAQDDRPEFLDPTLLGNGTNWQDAVFQIAPMNQHQISAQGGTDLIKYYVSGSYMNQQ
                     GTIIGTKFNRYSFRANLDAQLKKWFKLGLNSMYSMTHERLGLADSDEGIVKYSLLTPP
                     DIPIYDLDGNYASVVREGYTRINPIAMAMDEDILLDRNKLAGNIFAEINPYKPLVWHT
                     ELGFDIGGSRGEHFEPAVTYGNWSRKSNMSSIQNNFNKFWQFKNYLTYSGKSDKHNYT
                     AMLGQEMWESNYEYEGVSSTNLPSNDIHNPNLGSDPQINTGFGSSSMASFFGRATYNY
                     DDRYMGTYTYRRDGSSNFGPKNRWAGFHSFASSWRFTNEEFLKSITGVLNNGKIRFGW
                     GQTGNSNIGSYMWGAAIAKMPSGLGLGYRQRNLANPYIKWETQEQWNLGIDLNFFRDR
                     IGLVVDMYDKTSKDMLMALQLPSYMGTRGNPSSALASPYGNYGTINNKGVEFTLNTHN
                     LTGAFKWNTEFQMSFNKNKLVALDGTASAHIEGYGQWSDVVTITNVGDPLYNFYGYKV
                     VGVYQDLDDLKNSPKPEKYPSNGVFDRYNTTWVGDLKYADLSGPEGKPDGKINTYDRT
                     NIGSPMPKFNYGMTNTFNYKNFDLSVFINGTYGNKVYNYMAMNLSSMTSIWDNQLKVV
                     TERTLLEPINATKTYPAVINGVTVYNWFEDITNVKVSNPSATVPRAIANDPNDNNRIS
                     DRYIEDGSYLRIKNITFGYSLPAKISRQWKVDNIRVYANIQNLYTFSKYTGYDPEIGA
                     STASSNVYGLDNGRYPSPQVYSFGINLSF"
     gene            3200..4888
                     /locus_tag="PKH68_07185"
     CDS             3200..4888
                     /locus_tag="PKH68_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX12758.1"
                     /translation="MKRFNNISKYLIFTILISFGFVSCEEFLSRPTEDNYTVDNFYQT
                     DEQCFQAINPVYNSPWYDFQRGFFKIGEVLSGNYYWGSSPYLTFTINSTDEDLVNMSA
                     SLWSVNAYCNGIIENIDLKAGKNVKESTKNTVKGEALVWKAMAYFYLVRCFGEVPIIH
                     NNSAEIASGNYNNVYKATIPNVYDYIIMTLEKAIEWLPTSNQPGRIDRYSAYGLLSKV
                     YLTKSGYGMSGTRNASDLAKAAEYAKKVIDESGRSLLPVYSDVFRLKNNFSEESLLAW
                     HWVVSNNWTSQNTLQSDLGIVGFDEYGATWGGYAGPSVDLQQAFGENALNLTRNNNDA
                     RRKATMMMYGDKYDYFWVDKGGFDYTQFAVKSMEYQSATGANEVKHLVGNDNDHIIGC
                     GTSMSRMATSLSTHLLRLSDVYLIYAEAVLGNNATTSDPSALKAFNAVRGRSVQGYEP
                     KTSITWNDIWKERRLELACEGDRWYDYVRWHYYEPAKAIAELKAQKRSWYAGLNNYYK
                     TGTFNAADVYYDANPSIPNITDAHFKLPFPDTDLTMNPNLLKDPVEFDLSTIHY"
     gene            4908..6152
                     /locus_tag="PKH68_07190"
     CDS             4908..6152
                     /locus_tag="PKH68_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycan-binding surface protein"
                     /protein_id="HNX12759.1"
                     /translation="MKKYITTSKLAKSFILAFTVIIGFAFQACNDNPDKYEIADGVPE
                     VYYVRVPNPVSSDSLLVQAFMDNTICLVGHNLRSIREMWFNDQKAVLNSSLITDNALI
                     VTIPKTIPSVVTNKIYMITKTNDTIPYTFGVQVPSPVVNSISCEYAHDGDLATLYGDY
                     FIDDPNVPLTITMAGNVPVTEIKSIEKTKVVFKIPAGAQKGYMNVKSIYGTGRSHFQF
                     RDDRGIILDWDNTNANGGWRSGKIKNSDPAGISGNYVYFTGALDGDPTKDWAEDNFSF
                     NLWGTANGRPQGDLFSTPPASSVLKFEVNVTQAWSSCALQMIFTPWSTNGTNGYIADG
                     KTPRGLWRPWEATGSYTTDGWVTVAIPLTEFKYSHSGGSLEMAPAGNYGGLTFFVYNG
                     GITGTSCTPYICIDNIRVVPAE"
BASE COUNT         1907 a         1396 c         1353 g         1635 t
ORIGIN      
        1 atggctcagg aatcacgccg ggtaaccggg actgtcattg acggcaacga caatacagcc
       61 atgatcggag tcaatgttat tgaaaaagga accggtaatg gtacagtgac aaaagcagac
      121 ggaagttttg caattaatct gacctcaaaa aatccggtat tgatcttctc cagcctcgga
      181 tatacatccg ttgaagaggt tgtgggcaat cgcaccagtt ttaaaatagt actgaacgaa
      241 gaccggaaaa ccctcggcga agtggttgtg gttggttacg gtaccatgaa aaaaagcgat
      301 ttatcgggag ctgctgtcac tgtaggcgaa gataaaatca aaggttcaat cattacgaac
      361 ctcgatcagg ctttgcaggg acgtgttgcc ggtgtaaatt cggtgatgac ttccggagca
      421 ccgggttcat cagtgtctat ccgtgttcgc ggtcagtcga caatcaattc caatgcagaa
      481 cccctttatg taatcgatgg cgtcatcgtt cagggaggtg gcaacagcgg agcatcattc
      541 ggtctgggcg atgcacttgg caatgggagc acatcaacca tctctccgct ttcaaccatc
      601 aatccttctg atattgtctc catggaaatc ctgaaagatg cctctgcaac tgctatttat
      661 ggtgctcagg gttcaaacgg agtagttctg attactacga agaaaggaaa agctggagaa
      721 gctaaattct cttacgaagg tttgtttggc gtgcaacgtc aggctgcgcg catcgacatg
      781 atgaacctga gagaatttgc cgaatacagc aattcggtat cgcaggaaag caatgctcag
      841 gatgatcgtc ctgaatttct ggacccaacc ctgctcggca atggtacaaa ctggcaggat
      901 gcagtcttcc agattgcgcc gatgaatcaa catcagattt cggctcaggg aggtacagat
      961 ttaattaaat attatgtatc cggttcttat atgaatcaac agggaaccat tatcggtact
     1021 aaattcaatc gctattcctt ccgtgccaat ctggatgcac aattaaaaaa atggttcaaa
     1081 cttggattga acagtatgta ctccatgact cacgaaagac ttggtttagc cgacagtgac
     1141 gaaggtattg ttaaatactc acttctcacc cctccggata tacccatcta cgatctcgac
     1201 ggcaattatg caagtgttgt acgcgaaggt tatacccgta tcaacccgat agctatggcc
     1261 atggatgaag atatattact ggaccggaat aaactggcgg gtaatatttt tgccgaaatc
     1321 aatccctaca aaccattagt atggcatacc gaactaggtt ttgacattgg cggatcacgc
     1381 ggcgaacatt tcgaaccggc tgtaacctac ggcaactgga gccgcaaatc aaatatgagt
     1441 tccatacaga acaatttcaa caaattctgg cagttcaaaa actacctgac atacagtgga
     1501 aaatctgata agcacaacta tactgccatg ctcggacagg aaatgtggga atccaactac
     1561 gaatatgaag gggtatcttc caccaatctt ccttcaaacg atattcataa tcccaatctg
     1621 ggttccgacc cgcaaataaa cactggtttt ggcagttcgt ctatggcttc attctttgga
     1681 cgtgcaacct acaactatga tgaccgttac atgggtacat atacttatcg tcgcgatggt
     1741 tcatcgaact tcggacctaa gaaccgctgg gcaggtttcc attcattcgc ctcctcctgg
     1801 agatttacca atgaagaatt tcttaaatca attaccggtg tactcaataa tggtaaaatt
     1861 cgttttggct ggggacaaac cggtaattca aacatcggtt cgtacatgtg gggcgctgcc
     1921 attgccaaga tgccttcggg attaggtctc ggctatcgtc agagaaatct ggccaatccc
     1981 tatatcaaat gggaaacaca ggaacaatgg aaccttggca ttgacctcaa cttcttcaga
     2041 gaccgcattg gactggtggt tgatatgtac gacaaaactt cgaaagacat gcttatggcg
     2101 ttgcaattac catcctacat gggtacacgc ggcaatccat cttcggctct ggcttctcct
     2161 tatggcaatt atggtaccat taataataaa ggtgtggaat ttaccctcaa tactcataac
     2221 ctgacaggtg cattcaaatg gaatactgaa ttccagatgt cgttcaataa gaacaaactt
     2281 gttgcgctcg acggcactgc ttcagctcat atcgaaggct acggacagtg gagtgatgta
     2341 gtaaccatca ccaacgtggg cgatccgctc tacaacttct atggctacaa agtagtgggt
     2401 gtatatcaag acctggatga cctgaaaaat tcacctaaac ctgaaaaata tccttccaac
     2461 ggagtatttg accgttacaa tacaacatgg gttggcgacc tcaaatacgc tgacctaagt
     2521 ggtcccgaag gaaaacctga tggtaaaata aacacttacg accgtaccaa catcggttca
     2581 ccaatgccta aattcaacta cggtatgaca aatacgttca attataaaaa tttcgattta
     2641 tcggtattta tcaacggaac ctatggcaac aaagtgtaca attacatggc tatgaacctt
     2701 tcgagtatga caagtatctg ggacaatcag cttaaagtag taacagaacg caccctgctc
     2761 gaaccaatca atgccacgaa aacatatccg gcagtaatca acggtgtaac agtgtacaac
     2821 tggtttgaag atatcaccaa tgtaaaagtt tcaaatcctt cggctaccgt tccgagagct
     2881 atcgccaacg acccgaacga caacaatcgc atcagcgaca gatacatcga agacggatct
     2941 tacttgagga taaaaaacat cactttcgga tattcactgc ctgcaaaaat cagccgtcag
     3001 tggaaagttg ataacatacg agtatatgcc aacattcaga atctgtacac atttagtaaa
     3061 tacacaggtt atgatcctga aatcggtgca agtactgcaa gctccaatgt ttacggactc
     3121 gacaacggac gttatccttc gccacaggtt tattcattcg gaataaattt atcattctaa
     3181 ctttttaatt aaaataccta tgaaacgatt taataatata tcaaaatacc tcatttttac
     3241 gatactgatt agctttggtt tcgtttcgtg cgaggaattt ttaagccggc caaccgaaga
     3301 taactatacc gtagataatt tctatcagac cgacgagcaa tgttttcagg caataaatcc
     3361 tgtttacaat tctccctggt acgattttca gcgtggtttc ttcaaaatcg gtgaagtatt
     3421 gtcaggtaac tattactggg gttcgtctcc atacctcacc tttacaatca actcaacaga
     3481 cgaagacctt gtaaatatgt cggcttcact ctggtctgta aatgcctact gcaacggcat
     3541 tatcgagaac attgacctga aagccgggaa aaatgtaaaa gaaagtacaa aaaacaccgt
     3601 taagggcgaa gcactggtat ggaaagcaat ggcatatttc tatcttgttc gttgcttcgg
     3661 tgaagtaccc attattcaca acaattcggc tgaaatagct tccggcaatt acaacaacgt
     3721 atataaagcc actattccga acgtgtatga ctatatcatc atgacactcg aaaaagctat
     3781 cgaatggtta cccaccagca accaacccgg ccgtattgac cgttattcgg cttatggatt
     3841 actttctaaa gtatatctta caaaatcggg ttacggaatg tcgggaactc gcaatgcctc
     3901 tgatctggca aaagcagccg agtacgccaa gaaagtgata gatgaaagcg gacgcagcct
     3961 attgccagtt tattcggatg tttttcggtt gaaaaataat ttcagcgaag agagtttgct
     4021 cgcctggcat tgggtggtat ccaataactg gacatcgcaa aacacactgc aatctgacct
     4081 gggtattgta ggtttcgatg aatacggagc aacctggggt ggatatgcag gtccttctgt
     4141 tgatctgcaa caggcattcg gcgaaaacgc cctcaatctg acacgtaaca acaacgatgc
     4201 ccgccgtaaa gcgaccatga tgatgtacgg cgacaaatat gattacttct gggtggacaa
     4261 gggcggattc gactacactc aatttgctgt aaaaagtatg gaatatcaaa gtgctaccgg
     4321 agccaatgaa gtaaaacacc tggttggaaa tgacaacgac cacatcatcg gatgcggtac
     4381 tagtatgtcg cgcatggcta ccagcctgag cacacacctg ctccgcttgt cggacgtata
     4441 tctgatctac gctgaagccg ttctcggtaa caatgcaaca acatccgatc cttcggcact
     4501 gaaagctttc aatgctgttc ggggacgctc agtacaggga tatgaaccaa aaacatccat
     4561 cacctggaac gatatctgga aagaacgtcg tctggaactt gcctgcgaag gcgaccgctg
     4621 gtacgactat gttcgctggc attattacga accggcaaaa gccattgccg aattaaaggc
     4681 acagaaaaga agctggtatg ccggtctgaa taactattac aagacaggca cctttaatgc
     4741 tgcggatgtt tactacgatg ccaatccgtc aatacccaat attaccgatg cacatttcaa
     4801 acttccattc cctgatactg acctaacgat gaatcctaat ctgctgaaag atccggttga
     4861 gtttgattta agcaccattc attattaatc gcttataata acggaatatg aaaaaatata
     4921 taacaacaag taaactggct aaatcattca ttttagcatt tacagtcatc atcggatttg
     4981 catttcaggc atgcaatgac aatcccgata aatatgaaat tgccgatggc gtgccggaag
     5041 tgtactatgt acgcgtaccc aatccggttt cgtctgactc gctcctggtg caggcattta
     5101 tggacaatac catttgtctg gtgggacaca atttacggag cattcgcgag atgtggttca
     5161 acgatcagaa agcagtttta aattcaagtc taattactga taatgccctg attgttacca
     5221 ttcccaaaac aattccttcg gttgttacca acaagattta tatgataacc aaaacgaatg
     5281 atacaatacc ttacactttt ggtgtgcagg ttccaagtcc ggtggtaaac agcatttcat
     5341 gcgaatatgc tcacgatggc gatttggcca cattgtacgg cgattatttc atcgatgatc
     5401 ccaatgtacc gctaaccatt acgatggcag gaaatgtacc ggttacagaa attaaaagta
     5461 tcgaaaagac gaaggtagtc ttcaaaattc ctgccggagc tcagaaagga tatatgaacg
     5521 tgaagtcgat ttatggaaca ggacgttcgc atttccagtt cagagacgac cgcggaataa
     5581 tccttgactg ggataacacc aatgcaaatg gtggctggcg ttcgggaaaa attaaaaact
     5641 ctgaccctgc cggtatcagt ggtaactatg tgtactttac aggtgcactc gatggcgacc
     5701 caaccaagga ctgggcagaa gataatttct cctttaatct ttggggaaca gctaacggac
     5761 gtccgcaggg tgatttattc tcaacacctc cggcatcgtc tgtactaaaa ttcgaagtta
     5821 acgttactca ggcctggtct tcatgtgcgt tgcaaatgat ctttaccccg tggtcaacaa
     5881 acggtaccaa cggctacata gccgacggca aaacaccacg cggtttgtgg agaccctggg
     5941 aagctaccgg ttcatataca acagacggtt gggttacagt agcaattccg cttacagaat
     6001 tcaagtattc acattcgggt ggatcgctcg aaatggctcc agcaggcaat tacggaggac
     6061 taaccttctt tgtgtacaat ggtggaatta caggaacttc gtgcacacct tatatctgta
     6121 tcgataatat cagggttgtt cctgctgaat aacaaatgat gttttataaa acaattatta
     6181 aaaaaagact tttatgcaaa caaaaaagaa tatatggaca gcaggcctga tgatatgctt
     6241 attgctgcta tccggaattg gatttacttc atgcgacaaa gatgaagtgg a
//
LOCUS       DAOPZK010000114         6203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_15599_length_6203_cov_23.631425, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000114 DAOPZK010000000
VERSION     DAOPZK010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15599_length_6203_cov_23.631425"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PKH68_07195"
     CDS             complement(<1..175)
                     /locus_tag="PKH68_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX12760.1"
                     /translation="MFKELDPLLHSELRLAVMSILLSVAEADFVYIRETTKATAGNLS
                     VQIDKLSEAGYIEV"
     gene            complement(168..752)
                     /locus_tag="PKH68_07200"
     CDS             complement(168..752)
                     /locus_tag="PKH68_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12761.1"
                     /translation="MEEKPLNEKESLELITRMIKNTHQKLEEGNGIPFLVWGYTSIIV
                     SILVWYQLKITQDYLWNLLWFLIPLIGFPIQMLLQRKRERGIKTYLEKIIKYVWTVIG
                     VAGFVVSMGAMFYWKIPILFIIIVLMATGTAITGLIIQFKPIIISGFVSILLSFGCLI
                     FSHLYSILVFALVFFLMMVIPGHILNFKNRKRHV"
     gene            1010..3478
                     /locus_tag="PKH68_07205"
     CDS             1010..3478
                     /locus_tag="PKH68_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNX12762.1"
                     /translation="MKKYFLFLLLHVISVSVFANVTIFGRIIDAITQKPIEFVTVSVY
                     NPQTGKNVAGTLTNQDGAFSIPSVANGDYELRASVVGYNSISKPVKVSGKAVAMGNIP
                     LSEDAKTLKEIEVVGQGSQMRFDIDKKVFSVDQNIAAAGGSATDVLQNIPSVNVDNEG
                     NVSLRNNESVEVWINGKPSGLTAENRAQVLQQMPAESIEAIEVMTNPSAKFKPEGTAG
                     IINLVMKKNRKAGYYGSLSAGSMYTQGSKPGINAGASINYSSSKIDAFANIGHRQMNM
                     TGTGLTDRQNFNNGDTTLLNQSSEDKRSFGGTFFRAGVDYHLNSKNTIGISGFGHSGS
                     PSGTSNISYLLTDKSTAATLRNYTRDNSEVNSHGGYNLNLNHKYDFDTKGSNIITNLS
                     YSGHSRNGNDQFVQTDKLNTLNKLDINQTADNSNKELEAKTDFTKKIGENTRLELGWN
                     STWQNRISLADGYDNINKSEILSYYNKFTYQEQIHAAYATYGTRISNLSLQAGLRAEY
                     LQKQLTSTSKNLITSISETNKLDPQSYLELFPSVYMAYSLPKNNELQLNYTRRVNRPR
                     GRQINSFRDYSDATTISYGNPALLPEFASAFELNYVKNWDNHTLSASAYYRFTDDVIQ
                     NVRFMRNSIMESTFMNLTKQQNTGLELVSKNRLFKFVNLTTSVNLYHARLNEATYQDP
                     FNAAIKVNIPGNSNFSWDTRVLANLMFSRTFSGQITGMYSAPQIIAQGKRAQDYSVDL
                     GLRKTFMDKKINLNLMVRDLLNSRKMSATSWGDGFYQVSENRFSGRMIGLTVTYNFGN
                     MKPKPADRKNGNSTEPAVEGMDME"
     gene            3635..6151
                     /gene="feoB"
                     /locus_tag="PKH68_07210"
     CDS             3635..6151
                     /gene="feoB"
                     /locus_tag="PKH68_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNX12763.1"
                     /translation="MKLSELKTGEKASIVKVSGHGGFRKRIIEMGFVKGKVVESLLDA
                     PLNDPVKYRVMGYEVSLRRSEAQLIEVVSEEEALKARRTKDVLSPVMEEIEVRKQALN
                     KRKHIKVALVGNPNCGKTTLFNKVSGQHEHVGNYSGVTVDARQGVFEQDGYRFEIVDL
                     PGTYSLSAYSPEEKYVRSFIVDEMPDVVINIVDSSNLERNLFLTTQLIDMHLQTVVAL
                     NMYDELQKSGDKLDYTTLGKLLGIPFVPLIAKTGFGVVNLFRTVIKLYEGSNIISESG
                     LLIPAAKDDDLLDSYHHEIKIHHQHGNKNRFSDLEGEHKIHEIARHIHINHGPGLEKS
                     ILRLRHLLVSENHHLQTKYSLRYLSIHLLEKDKEIDKLISQESNASEIFRMRDEEINR
                     IAIELKTDAEAAITDAKFGFIAGALKETFSENKKKPANSTTSRLDAIVTHKYWGYPVF
                     ILFIYIMFQSTFSLGKYPMGWIESLVAWLNDVIHNLMADGPLKDLLVDGVLGGVGGVI
                     VFLPNILILYFFISFMEDTGYMARAAFIMDKIMHRMGLHGKSFIPLIMGFGCNVPAIM
                     ATRTIENRNSRMITILINPLMSCSARLPVYLLLAGTFFPRYAGLVLFGMYAAGILLAI
                     IIARLLKKFLFRGDESPFVMELPPYRVPTLKSMWNHTWEKGQQYLRKMGTIILVGSII
                     IWFLGYYPRSTQPEKPSTVNTTGQLENSYIGKIGHAIQPVLAPLGFDWKISVSLLSGV
                     AAKEIVVSTLGVLYSGNADEETASLSAKMKSEVRPNGLPVYDAGTALAFMIFVLIYFP
                     CLATVVAIKNETGSWKWALFAAGYSLVLAWMVAFLVQSIV"
BASE COUNT         1874 a         1312 c         1303 g         1714 t
ORIGIN      
        1 cgacttcaat gtaacctgcc tcgctcaatt tatcaatctg aacactcaga tttcccgcag
       61 tagcttttgt cgtttccctg atgtaaacga aatcggcttc agctaccgaa agtagaatgg
      121 acataacggc cagccggagt tcagaatgta gcagcgggtc aagttcctta aacatgcctt
      181 ttacggtttt tgaagtttaa aatatgcccg gggattacca tcatcagaaa aaacaccagg
      241 gcaaaaacca gtatggaata taaatgactg aaaatcagac atccaaaaga gagcaaaatg
      301 cttacaaatc cggatatgat gatcggtttg aactgaatga tcaagcctgt aatggctgtt
      361 cctgtagcca tcaatacaat tataatgaac agaatgggta ttttccagta aaacatggca
      421 cccattgaaa ctacaaaacc tgctacaccg attactgtcc agacgtattt gatgattttt
      481 tccagatacg tttttattcc tctttctctt tttctttgca gaagcatctg aataggaaat
      541 ccgatcagag ggatcagaaa ccacaggaga ttccaaagat aatcctgagt gatttttagc
      601 tgataccata caagtatcga aactataatg gaggtatatc cccaaaccag aaagggaatt
      661 ccattacctt cttcaagttt ttgatgcgtg tttttaatca tgcgagtgat aagttctaaa
      721 ctttctttct cgttcaaagg tttttcttcc attgagttaa ttatttgttg ggtaaattga
      781 attatgaatg tcgatgcaaa tgtaaatcgg tttataatat aaaccaaata taagtatcaa
      841 aatctgcatt taattaacat aaaataacta tagagttctt tttagccaaa atatccaata
      901 attttcctcg ttgtttaaac tttttccctt atcagttgtc atactctgat tattcattta
      961 ttgctgtgtg caggtaatat atccgcccgg taaagtcaaa acgattttta tgaaaaagta
     1021 ttttttgttt ttattgcttc acgttatttc tgtttcagtt tttgccaatg ttaccatttt
     1081 cggacgaatc attgatgcaa ttacacaaaa accaattgaa tttgtaactg tttctgtata
     1141 taacccgcaa acaggtaaaa atgtcgcagg tacattaacc aatcaggatg gcgctttcag
     1201 tattccttcg gtggcaaatg gagactacga actgcgtgca agtgttgtgg gttataacag
     1261 catttccaaa ccggtaaaag ttagcggcaa agccgttgct atgggaaata taccgcttag
     1321 cgaagatgcc aaaactctga aagaaattga agtcgtggga caaggctcac agatgcgttt
     1381 cgatattgac aagaaagtgt tcagcgtcga tcagaacata gctgcagccg gtggttcagc
     1441 tactgatgtg ctgcaaaata tcccttcagt caatgtggat aatgaaggca atgtctcact
     1501 tcggaacaac gaaagtgtgg aagtatggat aaatggaaag ccctccggac ttacggccga
     1561 gaaccgggct caggtactac aacagatgcc cgctgaaagt attgaagcca tcgaagtgat
     1621 gactaatcct tcggcaaagt tcaagcccga gggaacggcg ggaatcatta atctggtgat
     1681 gaaaaaaaac cgaaaagccg gatactacgg aagcctttcg gctggtagca tgtatactca
     1741 gggttctaaa ccgggtatta atgccggagc aagtatcaat tacagcagca gtaaaattga
     1801 tgcatttgcc aatattggac acagacaaat gaacatgacc ggaaccggat taaccgaccg
     1861 tcagaatttc aataacggtg acactacact actcaatcaa tccagtgagg ataaacgctc
     1921 attcggtgga acttttttca gagcaggagt ggactatcat ctcaacagca aaaatacaat
     1981 cggaatttca ggatttggac attcgggatc acccagcggt acatccaaca tcagttatct
     2041 gcttaccgat aaatcgacag ctgccaccct cagaaattat acccgcgaca atagcgaagt
     2101 taattcccac ggaggctata acctcaattt gaatcacaag tacgattttg ataccaaagg
     2161 atcaaatatt attaccaatc tttcttattc aggtcattcc cgcaatggca acgaccagtt
     2221 cgttcagacc gacaaactta atacattgaa caaactggat ataaatcaaa ctgccgacaa
     2281 ctcaaacaaa gaactggagg caaaaacaga tttcaccaaa aaaataggtg aaaatacccg
     2341 actggaactc gggtggaaca gtacctggca gaaccggatt tcgttagctg atggctacga
     2401 caatattaat aagagtgaaa ttctaagtta ctacaacaaa tttacctatc aggagcaaat
     2461 tcatgcagcg tatgcaactt atggaacccg catcagtaat ctttcgctac aggccggact
     2521 tcgtgccgaa tacctgcaaa aacaactgac aagcacttcc aaaaacctga ttacatcgat
     2581 ttctgaaact aacaaactcg atcctcagtc ctatttagag ttatttccaa gtgtgtatat
     2641 ggcgtattca ctgcccaaaa ataacgaact gcagttgaat tacacccgac gcgtcaatcg
     2701 tccccggggc cgtcagatca attcgttccg cgattattcc gacgctacaa ccatttctta
     2761 cggaaatccc gcactgcttc ccgaatttgc ctccgcattt gaactcaact atgtgaaaaa
     2821 ctgggacaat cacaccctgt cggcatcggc gtattaccgc tttaccgacg atgttattca
     2881 aaatgtgcgc tttatgcgaa atagtatcat ggaaagtaca tttatgaatt tgaccaaaca
     2941 gcaaaatacc ggactggaac tggtatccaa gaacagactc ttcaaattcg ttaatctgac
     3001 cacttcggtg aatttatatc atgccaggct aaacgaagcc acttatcagg atcctttcaa
     3061 tgccgccatt aaagtgaata tccccggcaa cagcaatttt tcgtgggata ctcgtgtgtt
     3121 ggccaatctg atgttttcac gcactttctc cggtcaaatt accggaatgt acagcgcccc
     3181 gcaaataatc gcgcagggca aacgggctca ggattattcg gtcgatctgg gattgcgcaa
     3241 aacctttatg gataaaaaaa ttaacctcaa cctgatggtg cgcgacttgc tcaattcgcg
     3301 aaaaatgagt gccacaagct ggggcgatgg tttttaccag gtttccgaaa accggttcag
     3361 cgggcgtatg atcggactca ccgttactta taactttgga aacatgaaac caaaacccgc
     3421 cgacagaaag aatggaaatt ccactgagcc tgctgtggaa ggcatggata tggaataatc
     3481 ctttactttc agagatatac aaacagcgct tcgtccttta cgaagcgttg tttgttttgt
     3541 taaaaattca agtattacac taaatctcca actttcttgt ttagaattaa tctatataag
     3601 ttatctttgc aaactcaaat gacagcagat acagatgaaa ctttctgaat taaaaacggg
     3661 tgagaaagcc tcaatagtaa aggtctctgg acacggaggt tttcgcaaac gaattatcga
     3721 aatgggtttt gtgaagggca aggtggtgga atcgctgctg gatgcaccgc tcaacgatcc
     3781 ggtgaaatac cgggtgatgg gctatgaagt ttccctgcga aggagcgaag ctcagctcat
     3841 cgaagtggta agcgaggagg aggctctcaa agcaagacgg actaaagatg ttctttcacc
     3901 tgtaatggag gaaatcgaag ttcggaagca ggcgctcaat aagcgtaaac atataaaagt
     3961 ggccctggtg ggtaatccca actgtggtaa aacaacactc ttcaataaag tatcgggcca
     4021 gcacgaacat gttggaaatt acagcggtgt cactgtggat gcccgacagg gtgttttcga
     4081 acaggacggt taccggttcg aaatagtgga cttgcccgga acatattcac tttcagctta
     4141 ttcgcccgaa gagaaatatg tccggagttt tattgtggac gaaatgcccg atgtggtaat
     4201 taatattgta gatagttcca atctcgaacg aaacctgttt ctcaccaccc aactcataga
     4261 tatgcacctg cagacggtgg tggcactcaa tatgtacgac gagttgcaga aaagcggcga
     4321 caaactggat tataccacac tgggaaaatt acttggaatt ccgttcgtac cactcatagc
     4381 aaaaaccggt tttggagtcg ttaatctttt tcgcactgta atcaaattat acgaaggaag
     4441 caatatcatc agtgaaagtg gattgcttat ccctgcagcc aaggacgatg atttgcttga
     4501 ttcctatcat cacgaaataa aaatacatca tcagcatgga aataaaaatc gcttctccga
     4561 tctggaaggt gaacacaaaa tccacgaaat agcccgtcac atccacatca atcatggccc
     4621 cggacttgaa aaaagcattt tacgtctgcg gcatttgctg gtaagtgaaa atcatcattt
     4681 gcaaacaaaa tattctctcc ggtacctttc cattcacctg ctcgaaaaag ataaggagat
     4741 tgataagtta atttcacagg aatccaatgc cagtgaaatc ttcaggatgc gtgacgagga
     4801 aattaacaga attgcaatcg aactcaaaac cgatgccgag gcagccataa ccgatgctaa
     4861 atttggtttt attgcgggtg cgttgaaaga aacattttcc gaaaacaaga aaaaaccagc
     4921 caatagtact acatcccgcc tcgacgccat tgttacccat aaatattggg gttatccggt
     4981 gtttatcctc tttatttaca ttatgtttca gtccacattt tcgctgggta aatatcccat
     5041 gggctggata gagagcctcg ttgcctggct caacgatgta attcataacc ttatggccga
     5101 tggaccattg aaggatttgt tagtcgacgg agtactgggc ggcgttggtg gcgttattgt
     5161 atttcttcct aatattctta tactgtattt tttcatttca ttcatggaag atacgggata
     5221 tatggcgcgt gctgccttta ttatggataa aatcatgcac cggatggggc tgcatggcaa
     5281 gtcattcatc ccactgatta tgggttttgg atgcaatgta cctgctatta tggctacccg
     5341 caccatcgag aaccgaaaca gccgcatgat tactatcctc atcaatccac tcatgtcgtg
     5401 cagtgcgcgg ttgccggtat atttgctgct ggccggaacg tttttccccc gctatgccgg
     5461 tttagtgctt tttggcatgt atgcagccgg tatcttgttg gccattatta ttgcccgctt
     5521 attgaaaaaa tttttattcc ggggcgacga atctccgttt gtaatggaat tgccgccgta
     5581 tcgggtgcca acactgaaat caatgtggaa tcatacctgg gaaaaggggc agcaatacct
     5641 ccgtaaaatg ggtacaatta tactggtggg ttctattatt atctggtttt taggatatta
     5701 tccccgatct actcaaccgg aaaaaccatc aaccgtaaat actaccgggc aactggaaaa
     5761 ttcctatatc ggcaaaattg gtcatgccat tcagccggta ttggcacctc tggggtttga
     5821 ctggaaaata agtgtatcac tcctttcggg tgttgcggcc aaagaaattg tggtaagcac
     5881 cctgggcgtt ctttattcgg gcaatgccga tgaagaaacc gcttcactgt cggccaaaat
     5941 gaaatcggag gtcaggccca atggtttacc agtctatgat gccggtactg cactggcttt
     6001 tatgattttt gtgttgatct attttccctg tttagccact gttgtggcca ttaaaaatga
     6061 aaccggatcc tggaaatggg ctttgtttgc tgccggatat tcgctggtgt tggcatggat
     6121 ggttgctttt ttagtgcaaa gtatagttta atgaacaaat tattaattga attgttataa
     6181 ttacagtttt ttccaatgca aca
//
LOCUS       DAOPZK010000115         6111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_15940_length_6111_cov_21.904557, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000115 DAOPZK010000000
VERSION     DAOPZK010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15940_length_6111_cov_21.904557"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..833)
                     /locus_tag="PKH68_07215"
     CDS             complement(9..833)
                     /locus_tag="PKH68_07215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNX12764.1"
                     /translation="MKSFLNITTILILIATMEACSTTKNMEPKVRMQTTYGDITVKLY
                     NETPLHRDNFLKLVNSGFYNGVLFHRVIADFMIQGGDPDSKAASPTDSLGNGDAGYTI
                     PAEFKTPTLFHKKGALAAARMGDAENPLKASSGCQFYIVEGKTFSDAELDNMQTNKLR
                     QQENSLFQEILKTRNEEVKKIRLERNQEKLDALRDSILLEVKNKIAADSTYKFSDKQR
                     TIYKTIGGTPHLDGNYTVFGEVTSGIDVVEKISKTNTGKMDRPKEDIRILKMEVVK"
     gene            complement(840..1592)
                     /locus_tag="PKH68_07220"
     CDS             complement(840..1592)
                     /locus_tag="PKH68_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNX12765.1"
                     /translation="MNTNFLDLTKTNWGEVKDRQYDMVLLPWGAIEPHNYHLPYITDA
                     ILTYEIARECAREAFTKANVMAMVMPPIYLGSQNPGQWEYPFCIHTNYETQKAILTDI
                     VKSLHGQGLRKMVIINGHGGNSFKAMIRDLAVVFPDFYIVSSDWYTFVPRTGYFDAKI
                     DDHAGEQETSVMMYFHPELVDLNTAGDGTPNPLKMKSITDKTGWMPRNWKETTEDTGI
                     GNPKKSTAQKGEIYVKEVINRITDLLTELKNL"
     gene            1746..3104
                     /locus_tag="PKH68_07225"
     CDS             1746..3104
                     /locus_tag="PKH68_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNX12766.1"
                     /translation="MGKIYEFTPSYMLAKGYLLFGFKRFYREFIVTGRENLPVDGSPV
                     IFAPNHLNALMDALAISTLVPRTKAVIYLARADLFAHKTMARLMHFAKIMPAFRMRDG
                     FENLEKNNRVFYQCVDVLRFSHSLCIMPEGGQGEERKIRPLVKGIFRVAFEAQQQFGE
                     AKKVKIIPVGINFGDLQKSGKQIIINIGTPIEVSDYMSGFLLNPVAATNEMRDALRNS
                     LSDLTLDMATAKYYKSFETISEIVSHEYIEPDKKANNTLEEFRLKQETIRKLIQIEND
                     DPDKMKDLYRLAGTYNRIIGKLKFKSGIFNQQKRYIPTFGLFLKLLLGLPVFLIGFLT
                     NFLPAMLPVAIRKLLRVSYPGFYTSVDFGLAMITFPVFYILQTVAAAAIFHLSWWMIL
                     IFIALQFFSRPFAVTWYSRTRKFIARLRFDGLLVQRSHAANLLYRAISVRNKIIYKLN
                     NA"
     gene            complement(3143..>3636)
                     /locus_tag="PKH68_07230"
     CDS             complement(3143..>3636)
                     /locus_tag="PKH68_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12767.1"
                     /translation="TFNQHLDPEKSDLILTDFDNAELWEGTSVYLIFWYGTFTDDIPV
                     VNPGILPLEITIAGNKSYFSASTFAENKKSMLLMLSFPDFTPSGSEGTDRSCISDKFL
                     TLAGNDEVLFSRIPVYQFYLAQSAGYFYLNALSIDFRKSFIETLSSDNAEPKILFFNL
                     MYL"
     assembly_gap    3637..3736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3762..4502)
                     /locus_tag="PKH68_07235"
     CDS             complement(3762..4502)
                     /locus_tag="PKH68_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12768.1"
                     /translation="MPQILQNAACFEIVVPPGMNKLSLEQVNLNGNLIRSVYLFSSTE
                     DVLMFSPSGSTQVTPLGETGQYGIFLSLTDVDNQEFIRNFSSGNFTVYSEMDHFLPYS
                     IHRKIDWAKSHLTTIIADDVLPLSLLMYVLYGDEEVVNVSAQNTVSKTVELIVNEDIQ
                     DFCLNDYFPELTDKRLTGISTAGRCPGYFDLTGNENRLENVPAHFFEIKSFKSFQFAP
                     MKIDLNQSWYRHRSFSHEPFSLTLSFLP"
     gene            complement(4572..6110)
                     /locus_tag="PKH68_07240"
     CDS             complement(4572..6110)
                     /locus_tag="PKH68_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNX12769.1"
                     /translation="MLVKTFGAAVQGINATIVTIEVNVGQGIRFMLVGLPDNAVRESH
                     ERIASALDFTGNKMPQRQVIVNMAPADIRKEGSAYDLPLAIGILAGDGKIPCEDLDKY
                     IIMGELSLDGGLQPIKGVLPIAIKAREDKFKGFILPRQNAREAAVVNNLDVYGVENIS
                     EVIEFFQGKSTLEPTIVNTREEFFQNLNLAEIDFSDVKGQDNVKRALEVAAAGGHNII
                     MVGPPGAGKSMLAKRIPTILPPLTLHESLETTKIHSVAGNIDSNTSLITQRPFRSPHH
                     TISDVALVGGGTFPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRKICISRAK
                     FSIEYPASFMLIASMNPCPCGYYNHPTRECVCAPGVVQKYLSRISGPLLDRIDLHIEI
                     VPVPFEKLSELKDSETSQQVRERVIKAREIQELRFRDIDGVYCNAQMSSKQQRTFAMP
                     DDEGNALLKNAMLRLNLSARAYDRILKVSRTIADLDDSEHVRAHHIAEAINYRNLDRE
                     GWAG"
BASE COUNT         1630 a         1308 c         1277 g         1796 t
ORIGIN      
        1 gtttcatctt atttcacaac ttccattttc agtatccgga tatcttcttt gggccggtcc
       61 atttttccgg tatttgtttt ggaaattttc tccacgacat caattcctga ggttacttct
      121 ccaaaaacgg tatagttacc atccaggtgc ggtgtgccac cgatagtttt gtaaattgtc
      181 cgttgcttgt cgctgaattt atacgtagaa tctgctgcga ttttattttt cacttccagt
      241 aaaatgctgt cacgcaacgc atccagcttt tcctgattgc gttccagccg gatttttttt
      301 acttcctcat ttctggtttt taatatttcc tgaaacaggg agttttcctg ctgccgcaac
      361 ttgttggttt gcatattgtc cagttcagca tccgagaatg ttttcccttc gacgatataa
      421 aactggcaac cggaggaagc tttcagcgga ttctcggcat cacccatgcg tgcagcggct
      481 aaggctcctt ttttgtgaaa aagggttggt gttttgaatt cggcgggaat ggtataccca
      541 gcatcgccat tgccgagcga atcggttggt gaggcagctt tagaatccgg atcgccaccc
      601 tgaatcatga aatcggctat cacacgatgg aaaagtactc cgttgtaaaa tccactgttt
      661 accagtttca ggaaattatc gcgatgaagc ggagtttcgt tgtacaattt tacggtaata
      721 tcgccgtaag tggtttgcat acggactttt ggctccatat tcttagtggt tgagcatgct
      781 tccattgttg ctataagtat taatattgtg gtaatattga gaaatgattt catagttttt
      841 tacaggtttt ttagttctgt caataaatca gtgatccggt taatcacttc ctttacgtaa
      901 atctcgcctt tttgtgcggt cgattttttt ggatttccaa tgccggtatc ttcggttgtt
      961 tctttccagt tacgtggcat ccagccggtt ttatcggtta ttgatttcat tttcaacgga
     1021 tttggagttc catcaccggc agtattcaga tccaccagtt cgggatgaaa atacatcatc
     1081 accgaggttt cctgctcacc ggcatgatcg tcaattttcg catcgaaata tcctgtccgg
     1141 ggcacaaaag tgtaccaatc ggagctaacg atataaaaat ccggaaaaac cactgccaga
     1201 tcccgtatca tcgccttaaa ggaatttcca ccatgtccgt tgataatgac cattttgcgc
     1261 aaaccctgac catgcagcga ttttacaata tcggtcagaa ttgctttctg agtttcataa
     1321 ttggtgtgaa tgcaaaacgg gtactcccac tgaccgggat tttgggaacc taaatagatc
     1381 ggaggcatca ccatggccat cacgtttgct tttgtaaaag cctcacgggc acactcccgt
     1441 gctatttcgt aagtcaaaat ggcatcggtg atgtagggta aatgataatt gtgcggttcg
     1501 atggctcccc agggaagcaa caccatatca tattgtctgt cttttacttc accccaatta
     1561 gttttggtta aatctaaaaa atttgtattc attcgtgatc gtttaattta ttatcatact
     1621 gttaattagc cacttaaaga tcgttacttc gggcaggtaa ttcacattca aagatattca
     1681 tttttcaagt tataattggt tgtaatagct atattttatt aaatttgcag ttaaaatcgt
     1741 tgataatggg taaaatatac gaattcactc catcatatat gcttgccaag ggatatttat
     1801 tatttggttt caagcggttt tacagagaat ttatcgtgac aggcagagag aatttgcctg
     1861 tggacggttc gcccgttatt tttgcaccca atcacctgaa tgctctgatg gacgctctgg
     1921 caatttcgac cctggttccg cgcacaaaag cagtgattta cctggcgcgg gctgatttat
     1981 ttgcacataa aaccatggcg cgcctgatgc attttgctaa aataatgcct gccttccgta
     2041 tgcgcgacgg ttttgaaaat ctggagaaaa acaaccgggt tttttaccag tgtgtggatg
     2101 tgcttcgttt tagtcattca ctttgcatca tgcccgaagg cggacagggt gaagaacgaa
     2161 aaatccgacc gttggttaaa ggaatttttc gggtagcttt tgaagcccag caacaattcg
     2221 gcgaagcgaa aaaagtaaaa atcatccctg tggggattaa ctttggcgac ctgcaaaaat
     2281 caggcaaaca gattattata aatatcggaa cgcccattga agtaagcgat tatatgtcgg
     2341 gatttctgtt aaatccggtc gcagcaacca atgaaatgcg cgatgcactg agaaacagct
     2401 tgtcggattt gacacttgac atggctaccg ccaaatatta caaaagcttc gagacaatat
     2461 cagaaattgt ttcgcacgaa tacattgaac ctgataaaaa agcaaataat acactggagg
     2521 aattcaggct aaaacaggaa accattcgaa agcttatcca aattgagaat gatgaccctg
     2581 ataaaatgaa agatttgtac cgtctggctg gaacttacaa cagaattatc ggcaaactga
     2641 aattcaaatc cggaattttc aatcagcaaa aaagatatat tcctaccttc ggattgttcc
     2701 ttaaactact cctcggttta cccgtgtttc tgatcggttt tttgacaaat tttctgcccg
     2761 caatgctccc tgttgcaata cgaaaactac tgagggtaag ctatccggga ttttatacat
     2821 cagtggattt tggtttggct atgattacat tcccggtgtt ttacattctt caaacagtag
     2881 ctgctgctgc catttttcat ctgagctggt ggatgatatt gatattcatt gctttgcagt
     2941 tttttagccg cccctttgcc gtgacctggt actcacgtac ccgcaaattc attgcccgtc
     3001 tgcgcttcga tggacttctt gtgcagcggt cgcatgccgc taacctcctc tacagagcca
     3061 tttctgttcg caacaaaatt atttacaagt taaataatgc ttaaaatctg aatttctgaa
     3121 tttttatttc cggttttctc ttttacaaat acatcagatt gaaaaacaag attttaggct
     3181 cagcattatc agaggaaaga gtttcaataa aagatttgcg aaaatcaatg gaaagtgcat
     3241 tcagataaaa ataacccgca ctttgagcaa gatagaattg atacaccgga attcgggaaa
     3301 ataaaacttc gtcgttacca gccagcgtta aaaatttgtc ggaaatacac gaacggtctg
     3361 ttccttcact ccccgaagga gtaaaatccg gaaaagaaag catcaacaac atactttttt
     3421 tgttttcagc aaaagtactt gctgaaaaat acgatttatt gccggcaatt gtaatttcca
     3481 ggggtaaaat tccgggatta acaaccggaa tatcatcagt gaaggttccg taccagaaaa
     3541 tcagatatac cgatgttccc tcccaaagtt ctgcgttatc gaaatcagta agtatcaagt
     3601 ccgatttttc ggggtcaagg tgctgattga atgtcannnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnccga ctgcaccgtt tatccgtctg ttcatggcag aaaacttaag
     3781 gtgagactga atggttcgtg cgaaaaactc cggtgacgat accaggattg atttaaatct
     3841 attttcatgg gtgcgaactg aaagcttttg aaagatttta tttcaaaaaa gtgagcaggt
     3901 acattttcaa gccggttttc attgcccgtc aaatcaaaat aacccggaca acgccccgct
     3961 gtggaaattc ccgtcagcct tttatcagta agttccggaa aataatcatt cagacaaaaa
     4021 tcctgaatat cttcatttac aatcaattcc actgttttgc ttaccgtatt ctgagcagaa
     4081 acatttacca cctcttcatc accatacaac acatacatca gcagacttag cggcaggaca
     4141 tcatcagcaa taatggtggt cagatggctt tttgcccaat ctatttttcg gtggatggaa
     4201 taaggcagaa aatgatccat ttcggagtag acagtaaaat ttccggaaga gaaattgcgg
     4261 ataaactcct ggttatcaac atccgtgaga ctaagaaaaa taccgtattg tccggtttca
     4321 cccaggggag tgacctgcgt actgcctgag ggggaaaaca tcagcacatc ttcggtggac
     4381 gaaaatagat acaccgatcg gattaggttt ccgttcaaat ttacctgttc caatgaaagt
     4441 ttattcattc cgggaggaac aactatctca aaacaagcgg cattttgcag tatctgtggc
     4501 atgaatattt attgatcaat aaatttaccc attgaaatga gaaagtatcc gtaattcgtt
     4561 gaaagtggag atcaccctgc ccagccttcc ctgtcgagat ttcggtaatt gatggcttca
     4621 gctatgtggt gcgcccgtac gtgttccgaa tcatccaaat cggcaatggt acgtgacact
     4681 ttgaggatgc ggtcatacgc cctggccgaa aggttcaagc gcaacatggc atttttgagc
     4741 agtgcattcc cttcatcatc gggcatggca aaagtgcgct gctgcttgga agacatttga
     4801 gcattgcaat acaccccgtc gatatccctg aaccgaagtt cctgtatctc acgggctttg
     4861 attacccgct cccgaacctg ctggctggtt tcagagtcct tcagttcgga gagtttttcg
     4921 aaaggtacgg gtacaatttc gatatggaga tcaattctgt caagcaatgg acccgaaata
     4981 cggctgagat atttttgaac cactcccggt gcacaaacac attcacgggt gggatggttg
     5041 taatatccgc agggacaggg attcatcgag gcgataagca taaaactggc gggatactcg
     5101 atggagaatt ttgcccgtga gatgcatatt ttccggtctt cgagcggctg acgcatcact
     5161 tccagtactg ttcttttgaa ttcaggcaat tcgtcgagga aaagcacgcc attgtgagca
     5221 agtgatattt cgccgggctg cggaaatgtg cctccgccca ccaaagctac atcagatatg
     5281 gtatgatgcg gacttcggaa cggccgctgc gtgattagcg atgtgttgct gtcgatattt
     5341 ccggctactg agtgaatttt cgttgtttcg agtgattcat gcagcgtgag tggtggtagt
     5401 atggttggaa ttcgcttggc caacatggat tttccggcac ccggagggcc taccataatg
     5461 atgttgtgac caccggcagc cgccacttcc agggctcgtt tcacattatc ctgccctttt
     5521 acatcgctaa aatcaatttc agccagattg agattctgaa aaaattcttc gcgggtattc
     5581 acaattgtcg gttccagtgt cgattttccc tggaagaatt caatgacttc ggagatattt
     5641 tcaacaccat atacatccag attattcact acggctgctt cgcgggcatt ttgtctgggt
     5701 agaataaatc ctttgaactt gtcttcacgg gctttgatag caatgggaag tacgcctttg
     5761 ataggttgca atccgccgtc gagcgaaagt tcacccataa tgatgtactt atccaggtct
     5821 tcgcacggaa tttttccatc accggccaga atgccaatag ccagcggtaa atcatacgcc
     5881 gaaccttcct tgcgtatatc ggcaggcgcc atatttacaa ttacctgacg ttgaggcatt
     5941 ttgtttcctg taaaatccag ggccgaagca atccgttcgt gactttcacg cacagcgtta
     6001 tcgggtaacc ccaccagcat aaatcttatt ccctgcccca cattcacctc gatagttact
     6061 atcgttgcgt taatgccctg aacagcagca ccaaatgttt ttacaagcat a
//
LOCUS       DAOPZK010000116         6109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_15949_length_6109_cov_25.948794, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000116 DAOPZK010000000
VERSION     DAOPZK010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15949_length_6109_cov_25.948794"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1152
                     /gene="thiL"
                     /locus_tag="PKH68_07245"
     CDS             130..1152
                     /gene="thiL"
                     /locus_tag="PKH68_07245"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNX12770.1"
                     /translation="MTEISKYGEFGLIKHLTEKIKLQNSSSLKGVGDDAAVLDYKDKR
                     VLVTTDLLLEGIHFDLTYTPLKHLGYKAAVVNFSDIYAMNGQPKQITVSLGVSKRFSV
                     EDLEEIYAGIYLACEKYGVDLVGGDTSASLTGLTISITCIGEGEEGKIVYRNGAKAND
                     LICVTGDLGSAYMGLQLLEREKMVFSNHSENIQPDFEEKKYILQRQLKPEARKDIIDA
                     LRKKGIVPTAMMDISDGLSSELIHICSQSNTGCRIYEDKIPIHYEAAVMAEELNMNLV
                     TAALNGGEDYELLFTVSLDDYEKIIPIEGVAIIGHITTPELGLNLVGREGEEIGLKAQ
                     GWNSLT"
     gene            1195..3438
                     /locus_tag="PKH68_07250"
     CDS             1195..3438
                     /locus_tag="PKH68_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNX12771.1"
                     /translation="MKYSLQLFLIFLGLATFQPTFPAGLPVIPKPNRIELLSGFFDLT
                     KPVKIKSEGISNASTRRFEDYLKSAGFKISNEQEAVIELCIDRKNSLLTNPEAYLLKI
                     EPSKITITAAKENGLFYGIQTLLQLVSEYGANKQIPALNITDSPRFPYRGMHLDVSRH
                     FFPMDFIKKQLDMMAYYKLNRFHWHLTDGPGWRLQIKKYPLLTEVAAWRTHKTWKDWW
                     ATNRQYVNRTDSGAYGGFYTQDEAREIVRYAAERHITVIPEIEMPGHSEEVLAVYPQL
                     SCSGIPYKNSEFCPGKEATFTFLENVLTEVMDVFPSEYIHIGGDEANKEPWKTCPNCQ
                     KRLKDEGLKNVDELQSYLIRRIEKFVTKHGRKIIGWDEILEGGLAPNATVMSWRGENG
                     AIEAARTGHNAIMTPGAFCYFDFYQNEPVNQAEAIGGYLPLEKVYSYNPVPAGLTPTE
                     AKHITGVQANLWTEYISTPERAEYQLYPRLLALAEVAWTMPENKSWNDFNRRVNDAIP
                     YLQCKGYHPFTLSGEVTFVQDVDSIRKCILVTLSTEKYGKEIRYTLDGTLPGPASAFY
                     SEPIIVTDSALITAQVFDRGEVVGKPVAKRFDYHRAIGNKVIYNIPINSYYPAAGLAS
                     LTDGRTGGLTHWDGRWQGFMTNGMDVTIDMQRVVPVRKISARFMQNAGTWIYFPTEVV
                     ISISDDNKTFTEIAHIANSYEVKTAGTIFHDFSWKGNAKGQFIRYQGLPNDIKGGWVF
                     LDEIVVW"
     gene            complement(3565..4608)
                     /locus_tag="PKH68_07255"
     CDS             complement(3565..4608)
                     /locus_tag="PKH68_07255"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HNX12772.1"
                     /translation="MNQKKSVLLIFTGGTISMTADPDTGALRPADFEVMMKLLPELGE
                     LGIDIHGIPLLPLVDSSDIGPDTWERIALVIQDNYNLFDGFVVLHGTDTMSYSASALS
                     FMLDGLTKPVIFTGSQLPIGVLRTDAKENLITAVEIAAAHSNGDAMVPEVCLFFEDKL
                     FRANRTTKQNSEHFNAFASFNYPILAEAGVHIKYFPNYIRKPNNTTRLKVRTKTDRNV
                     AILKIYPGITEQTVQGVLNIPELKAVVLETFGAGNAPRFDWFYRALKSAADRGIIIVN
                     KSQCSTGMVEMGLYETSLNLINAGVISGYDSTTEALITKLMYLLGENDDVETVKRLMV
                     TNLCGEITTHPSP"
     gene            4791..5318
                     /locus_tag="PKH68_07260"
     CDS             4791..5318
                     /locus_tag="PKH68_07260"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNX12773.1"
                     /translation="MRIFLIGYMGSGKTTIGRLVAEQTGLNFVDTDSYIENQQQKTIP
                     EIFAAMGEEKFRELERECLHEVSELEDMIISTGGGMPCHFDNMALMNRSGETIYIRFT
                     PEELTARLKTTQIHSRPILGSVPLNDLLPFITKNLHIREPFYNQAKFIVGGTDEDIFR
                     QICEYIHLHKKTCHI"
     gene            5338..5613
                     /locus_tag="PKH68_07265"
     CDS             5338..5613
                     /locus_tag="PKH68_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12774.1"
                     /translation="MKKPILILVYILLITGIILCITGAVNDFSTFLYQTGIAIFSFGW
                     IIVFFDMLKNKIHNKQFWVLSMFFVIPVTPIIYLIRRESLLSTGEIK"
     gene            5790..>6109
                     /locus_tag="PKH68_07270"
     CDS             5790..>6109
                     /locus_tag="PKH68_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HNX12775.1"
                     /translation="MKNNNKHNGFRYGENFLTIGTALKLARNDIKGFLTPETISRVQA
                     NRDVVLKIVHKKKVVYGINTGFGPLCTSLISKEDTKKLQYNLLMSHSVGVGNFIDKEI
                     AKLML"
BASE COUNT         1800 a         1342 c         1333 g         1634 t
ORIGIN      
        1 aggtgcgata ttatggtatt ataaaaaaag tcaagacgaa ctcacataaa taaaacgata
       61 tcaataactg caataataca ttaacgctaa actaaaaaag cctcctcatg ggggaggttg
      121 gaggggctta tgactgaaat atcaaaatac ggtgaatttg gattaatcaa acaccttacc
      181 gaaaaaataa aattacaaaa ttcttcatcg ctcaaaggtg ttggagatga cgctgctgtg
      241 cttgattata aggacaaacg cgtactggtc actaccgatt tattgttgga aggcattcat
      301 tttgatctga cctacacacc gctgaaacac ctgggttaca aagctgctgt tgtcaatttc
      361 tccgacatat atgccatgaa cggacagccg aaacaaatca ccgtttcgct gggcgtttcg
      421 aaacgttttt cggtggaaga tctggaagaa atatatgcgg gaatttatct tgcctgcgaa
      481 aaatacggcg ttgatctggt gggtggcgac acttctgctt cgcttaccgg cctgactatc
      541 agcatcacct gcatcggtga gggcgaagaa ggcaaaattg tgtaccgcaa tggtgcaaaa
      601 gcaaatgatc tgatttgtgt aaccggtgat ttgggttctg catacatggg cttacaacta
      661 ttggaacggg agaaaatggt tttttcaaat cattcggaaa atattcaacc ggatttcgaa
      721 gaaaaaaaat acatcctcca acgccagctg aaaccggaag cccggaaaga cattatcgat
      781 gcactgcgta aaaaaggaat tgtacccact gccatgatgg atatttcgga cggattatcc
      841 tccgagttaa ttcatatttg cagccagagc aacaccggat gtcgtatcta tgaagataaa
      901 attccgatac attacgaagc agctgtgatg gctgaagagt tgaatatgaa cctggtaact
      961 gccgccctca acggaggtga agattatgaa ttgctgttta ccgtttcgct ggatgattac
     1021 gaaaagatca tacctatcga aggtgttgca atcatcgggc acatcacaac gcccgaactg
     1081 gggcttaacc tggtaggacg tgagggagaa gaaatcggtc tgaaagcaca gggctggaat
     1141 tcgcttacct aaaaacatac aatatcgctt ccgggataaa taaggataca aactatgaaa
     1201 tattctttgc aactgttttt gatttttctt ggtctggcaa cttttcagcc gaccttcccc
     1261 gccggtttac ctgttattcc aaaacccaat cgcattgaat tgttgagtgg tttttttgat
     1321 cttacaaaac cggtaaaaat aaaatcggaa ggaatttcga atgcttcaac ccgtcgcttt
     1381 gaggattacc tgaaaagtgc tggtttcaaa atcagtaatg aacaggaagc tgtgatagaa
     1441 ctctgcattg accggaaaaa ttcgctgctc accaatccgg aagcctattt actgaaaata
     1501 gagccctcaa agattaccat tactgcggca aaagaaaacg ggctgtttta cggtattcag
     1561 actttattgc aacttgtcag cgaatacgga gcaaataaac aaattcccgc cttaaacatt
     1621 acagacagtc cgcgttttcc ctaccgggga atgcatctcg atgtctcccg ccactttttc
     1681 ccgatggatt ttatcaaaaa acagttggac atgatggctt attataagct gaaccggttt
     1741 cactggcatc tgaccgatgg tccgggctgg cgtctgcaaa taaaaaaata cccactcctc
     1801 accgaagtgg cagcctggcg cacgcataaa acatggaaag actggtgggc aacaaaccga
     1861 cagtatgtca accgtacaga ttccggtgct tacggtggtt tttacacaca ggacgaagcc
     1921 cgcgaaatag tccggtatgc agctgaaaga catattaccg ttatccccga aatagaaatg
     1981 cccggacatt cggaggaggt gctggctgtc tatccgcaac tttcatgctc gggtattcct
     2041 tacaagaaca gcgaattctg cccgggaaaa gaagctacat ttactttcct cgaaaatgta
     2101 ttaacggagg taatggacgt ttttccatct gaatacatcc acattggtgg cgacgaagcc
     2161 aataaagagc cctggaaaac ctgtcccaac tgccaaaaaa ggctgaaaga cgagggactt
     2221 aaaaatgttg atgaacttca gagttatctc attcggcgga tagaaaaatt tgtgaccaaa
     2281 cacgggcgta aaatcatcgg ctgggatgaa attctggagg gtggcctggc tccaaatgcc
     2341 acagtcatgt cgtggcgggg cgaaaacggc gccattgaag ctgcacggac aggtcataat
     2401 gccatcatga cgcctggcgc cttctgctac ttcgacttct atcagaacga accggttaat
     2461 caggcagaag caatcggcgg atacctcccg ttggaaaaag tctacagtta taatcctgta
     2521 cctgccggcc tcactcccac ggaagcaaaa cacatcacgg gcgttcaggc caatctctgg
     2581 actgaataca tctccacacc cgaacgggct gaataccaac tttacccgcg tctgctcgcg
     2641 cttgccgaag tagcctggac tatgcccgaa aataaatcgt ggaatgattt caaccgacgc
     2701 gtgaatgatg ctattccgta tctgcaatgc aagggatatc atccgtttac cctttccggg
     2761 gaggtgactt ttgtacagga tgtggatagt ataagaaaat gtatactcgt cacactttca
     2821 actgaaaaat acgggaaaga aatccgttac acactggatg gtaccctacc cggtccggct
     2881 tcagcttttt attcagaacc gattattgtg accgactcag ccctgattac agcacaggtt
     2941 ttcgatcgag gagaagtcgt gggtaagccg gtcgctaaac gatttgatta ccaccgggcc
     3001 atcggcaata aagtcattta caacatcccc atcaactcct actaccccgc tgccggactt
     3061 gcttcactca cagacggacg cacaggcggc ctgacccact gggacggacg ctggcaggga
     3121 tttatgacga atggtatgga cgtgaccatc gatatgcagc gtgtggtgcc ggtaaggaaa
     3181 atcagcgccc gcttcatgca gaatgcaggc acatggattt atttccccac tgaagtggtt
     3241 atttccatat ccgatgacaa caaaaccttt actgaaattg cacatatagc caattcgtat
     3301 gaggtgaaaa cagccggaac catttttcat gatttcagct ggaaaggtaa cgcgaaagga
     3361 caatttattc gttatcaggg actaccaaat gacattaaag gcggatgggt ctttttggat
     3421 gaaatagtgg tttggtaaaa gccccctcca actcccccga agggggagag aaacccctaa
     3481 cccctaaagg ggaataagaa agaaaaaaag ctttgagcac cgaaggtgta attgaatttt
     3541 tccgggaatg tgtcttgtcc ccctttaggg gctagggtgg gttgttatct ccccacacaa
     3601 attcgttacc atcagtcttt tcaccgtttc cacatcgtca ttttcgccca gcaggtacat
     3661 cagttttgta atcaacgctt cggttgtact gtcatatccg cttatcacac ccgcatttat
     3721 gaggttcaga ctggtttcgt acaatcccat ttccaccata ccggtgctgc actgcgactt
     3781 gtttacaatg ataattcccc ggtcggcagc acttttaagt gcgcggtaaa accagtcgaa
     3841 acggggcgca ttccctgctc cgaatgtttc gagcactacc gctttcagtt cgggaatgtt
     3901 cagaacaccc tgcacagtct gctctgtaat gccgggatat attttcagaa tagccacgtt
     3961 gcggtcggtt ttggttctca ccttgagcct ggtagtatta ttgggtttcc ggatataatt
     4021 ggggaaatat ttgatatgaa cacccgcttc ggccagaatg gggtaattga atgaagcaaa
     4081 ggcattgaaa tgctcggaat tctgtttggt agtccgattg gcccggaaga gtttatcctc
     4141 gaaaaacaga cacacttcag gcaccatggc atcgccgttg gagtgggcag cggctatttc
     4201 cactgccgta atcaggtttt ccttggcatc ggtacggagc acaccgatgg gcagctgcga
     4261 cccggtaaaa atcacgggtt tggtaaggcc atcgagcata aaactcagtg ccgaagccga
     4321 ataagacatg gtatctgtgc catgaagcac cacaaatccg tcgaacagat tgtaattgtc
     4381 ctgaattacg agggcaatac gctcccaggt atccggtccg atgtccgatg aatcgaccag
     4441 cggtagcagc ggaatgccgt gaatatcgat acccagttca cccaattcag gcagcaattt
     4501 catcatcact tcgaaatcgg cgggacgtag tgctcccgta tccggatcag cagtcatgct
     4561 gatggttcca ccggtgaata tcaataagac agattttttt tggttcattg gatttttgat
     4621 aagtaattca cctacaaaga tacatgtttt tgatattatt gtatcatatt gtcattattt
     4681 tatttgtcta aaattcctat ttctgatttt aaagaaaact caggcagtcc tgaacagaaa
     4741 attgcagaaa aagccgtatt tttgtattca tttttaacat gtaaaatacc atgcgaattt
     4801 tccttatcgg gtatatgggt tcgggcaaaa ccaccatcgg acgactggtg gctgagcaga
     4861 caggtttgaa ctttgttgat acagacagtt acatcgaaaa tcagcaacaa aaaaccatcc
     4921 cggagatctt tgctgcaatg ggagaagaaa aattccgtga gcttgagcgg gaatgcctgc
     4981 atgaagtttc ggaactggag gatatgatca tttccaccgg tggcgggatg ccctgtcatt
     5041 tcgataacat ggcgctaatg aaccgcagtg gcgaaaccat ctatatccgt tttacacccg
     5101 aagaactcac cgcgaggctt aaaacaactc aaattcacag tcgtcccatc ctgggaagtg
     5161 tccccctaaa tgatttactt ccttttatca ccaaaaatct ccacatccgc gaaccgtttt
     5221 acaatcaggc aaaatttata gttggtggaa ccgacgaaga tattttccgc caaatctgcg
     5281 aatatattca tctgcacaaa aagacatgtc atatttaatt catttaaatt atttattatg
     5341 aaaaaaccaa tcctgatttt agtgtacatt ttattgatta ctggaattat tttgtgcatt
     5401 acaggagctg taaatgattt ctcaactttt ctgtatcaaa ctggaattgc tatatttagc
     5461 ttcggttgga ttattgtctt ttttgatatg ctgaaaaata aaattcacaa caaacaattc
     5521 tgggttcttt cgatgttttt cgtaataccg gttacgccaa ttatttatct tatccgaagg
     5581 gaaagtttac tttctaccgg agaaataaaa taaacaattt tctgatattt tttacttaaa
     5641 atacaaacaa taaaaaggca catccattaa atgtgtcttt tttttgtgtt ttaaatttta
     5701 agccaaaagt aatttaatta tatttgcatt tatattgaat gaatattctt atttgttaat
     5761 actgccttta attaaatagc tgatacagaa tgaaaaacaa caataaacac aacggatttc
     5821 gctacggcga aaattttctt accattggca cagcgttgaa gcttgcccgc aacgatatta
     5881 aaggctttct cactcctgaa accatctccc gtgtacaggc caatcgtgat gtggtactca
     5941 aaattgtcca taagaaaaaa gtggtgtacg gcatcaacac tggtttcggt ccgctttgta
     6001 catcgctgat aagcaaggaa gacaccaaaa agctgcaata caacctgcta atgagccaca
     6061 gtgtgggagt tggcaatttt atcgataagg aaattgccaa actgatgct
//
LOCUS       DAOPZK010000117         6069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_16113_length_6069_cov_20.786498, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000117 DAOPZK010000000
VERSION     DAOPZK010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16113_length_6069_cov_20.786498"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKH68_07275"
     CDS             <1..887
                     /locus_tag="PKH68_07275"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX12776.1"
                     /translation="GDSTYCPGETVTLKGSGAHHYRWFNGSTADSVTIGRDTTVWMIG
                     YSSSNCRTDTIKMNIKQEPDWAFTTTGSETFCSGGSTVLSASGAVSYNWSTGAITDSI
                     RVSTPGTYTVTGKNPRGCLKQKTFIVTEDPLPDVNFELSATKIDVHNNELTGSIASQN
                     GVIYNWDMGDGKTEKGAKIIHKYEVDPDAVNYTVTLTAVNANGCENSASKTIGVVLFI
                     PNVFTPNGDGMNDKFMEGKNIKVFDRNGNILYEGNKGWDGYYKGKKADNDTYFYYIKY
                     TDIDSTEKTAKGFIMLKR"
     gene            900..2345
                     /locus_tag="PKH68_07280"
     CDS             900..2345
                     /locus_tag="PKH68_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HNX12777.1"
                     /translation="MNKKRTILLLSAIAVFFSLQAQTTTYGDTIFFDNFGEHTHRVEC
                     LYMPAGSYTFASENGDRNQREIQDNYYAVVDPRNIRDAITSDYSFFWTAPTLSAVTSS
                     GSTRFYTADHTPGDINGAVMVVNAGETVNYIYKRNLTHKTGYRYLLSFWIYVVAKSSQ
                     FSMEATNTTTGKTYIYTGPLLTDEGKWIKYDLNFSVPISANMTTNEVTIGLQNRNKLI
                     TGNDYYIDDILVSTLHTDPLTIDVSNTGPVCEGSPIQLIVNSGGDALPFTYAWTGPNG
                     FNSAESSPILSNSTANMSGVYNVTVTDALGQVATATTNVIVNTVPKSDFSVSGNTIDN
                     KNNIVTFSITPEDGVEYEWDLGDGTKEKGSTFTHTYSIDGNTYEYKIQLKSTISQGCS
                     STTSKTIEVTPFVPNVFSPNGDGVNDHFMTGFDTQIFDRSGNLLFSGTSGWDGNYHGR
                     EMDNDTYFYQIKYTDKYREVSNLKGFVILKR"
     gene            2353..3306
                     /locus_tag="PKH68_07285"
     CDS             2353..3306
                     /locus_tag="PKH68_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNX12778.1"
                     /translation="MKRQLYIGLLLIGYLASGNHIFSQTDISMATHWYNRGNYNPASI
                     ARTDYIYLFSNVRSQWTGITGAPRVANVQVSGYFNYINSAFGLSAVNDRIGITNALNP
                     MLNYAYRLSNDQSWSLAFGVGLGAYIRTIDISSYAPVTDLDPALMHDVAPQTKPDANI
                     GVEFQNASFIFGLSSTHLFSVFKDSTLFLNANHRYGYAIYKNTKSEVLNYYLGVQVVN
                     RSNIFVVEGNGIVRFKHPTGLSTGSREIFDLGLTYRSTKQLTALLGVNLTENFRVGYA
                     YDQSFITGYNQNGSHEIMLEYRIPYLNAVCKTCRDQQLWYR"
     gene            3415..5697
                     /locus_tag="PKH68_07290"
     CDS             3415..5697
                     /locus_tag="PKH68_07290"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNX12779.1"
                     /translation="MDKKKFDVVGMTCSACVAHVEKSVRKLNGVQEVNVNLLTNSMTV
                     DYDQQVLSPEDIILSVEDAGYEAFEKEQTTAKATTPGINHALEEQKEMKKRVINSFLF
                     LVPVLYLSMGSMAGIPIPSFFVGEQNALTFAFTQLLLTIPVYFINRKFFTHGFRSLWQ
                     LSPTMDALVAIGSSAALLYGIFAIYRISYGLGHQQLHLVHTYAHDIFFESGATILTLI
                     TLGKYFEAKSKRQTSEALTKLINSAPQTANVIRKGNEIEIPVEEVRLNDIVSIRPGEK
                     IAVDGIVESGSGSVDQSALTGESIPVFKQTGEPVLSASINKTGYFTFRATKVGKDTTL
                     SQIIQLLEEASASKAPISKLADRISRIFVPVVIGIALLATIVWLLLGYPFEFALSIGI
                     AVLIISCPCALGLATPVAIMVGTGKGAENGVLFKSAEALEMGDKIDTIIFDKTGTLTE
                     GKPKVTNVEALHYLNEEELLKISASLERYSEHPVSEAILQKAKELKYALYEVTAFENT
                     PGKGISGKINGQVYKIGNLSFISENTNTEDMDYLIHQYAQSGKTPLIITDDENILGII
                     AVADTVKPTSREAVTLLKNMNLKTIMLTGDNKRTALAIQEELGLDEVMAEVMPQDKDK
                     IVQQLQTEGKKVAMVGDGINDSPALMRADLGFAIGSGTDIAIDAADVVLMKSDLLDVV
                     TGIRLSKAVIKNIKENLFWAFFYNIIGIPLAAGVFYTALGWKLSPMFAAAAMSLSSVT
                     VVMNALRLKNFKPTKSHVIN"
     gene            5724..5933
                     /locus_tag="PKH68_07295"
     CDS             5724..5933
                     /locus_tag="PKH68_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNX12780.1"
                     /translation="MEKTAMIKGMSCSHCAARVEKALNKIDGVEARVDLESRSAKLIL
                     TKDVSDKTIKEAVEAIDYEVTDILE"
BASE COUNT         1871 a         1222 c         1329 g         1647 t
ORIGIN      
        1 cgggagattc cacctattgc cccggcgaaa cggttacact aaaaggctcc ggtgcacatc
       61 actaccggtg gttcaacggc tctacagccg attctgtgac aatcggcagg gatacaaccg
      121 tttggatgat agggtactcc agcagcaact gtcgaacaga tacaataaaa atgaacatca
      181 aacaggagcc ggactgggct tttacaacca cgggctcaga aacgttttgt tcgggtggaa
      241 gtacggtttt gtcagcctcg ggtgcagttt catacaactg gagcacgggt gcaattaccg
      301 attcaatccg cgtgagcact ccgggaacat atacggtaac cggcaaaaat ccacgcggtt
      361 gtctgaaaca aaaaaccttc attgttaccg aagatcccct gcctgatgta aactttgaac
      421 tttcggcaac aaaaattgat gtccataaca atgagctgac cggcagtatt gcatcgcaga
      481 atggtgttat ctacaattgg gacatgggtg acggaaaaac cgaaaaaggg gcaaaaatta
      541 tccataaata tgaggttgac cctgatgccg taaactacac tgttacatta acagcagtga
      601 atgccaacgg gtgcgaaaac tcagcttcaa aaactatcgg tgttgtactt tttatcccca
      661 atgtttttac tcccaacggt gatggtatga atgataaatt tatggaggga aagaatatca
      721 aggtattcga cagaaacggg aatatacttt acgaaggaaa taaaggttgg gatggatact
      781 acaagggtaa aaaagcagat aacgacacct atttctatta tatcaaatac accgatattg
      841 acagtactga aaaaactgca aagggattta ttatgctgaa aagataaata acaaggataa
      901 tgaataaaaa acgaaccata ttgttattgt ctgctattgc cgtttttttt tcgctgcagg
      961 cacagaccac gacttatggt gacaccatat tttttgacaa tttcggtgaa cacactcacc
     1021 gggtggagtg tctgtatatg ccagccggaa gttacacttt tgccagtgaa aacggagacc
     1081 ggaatcaacg ggaaattcag gataattatt acgcagtggt agatccaagg aatataagag
     1141 atgccattac ttcggattat tcttttttct ggacagctcc tactctttct gctgtcacat
     1201 cttccggttc cacccgtttt tatacagccg accacacgcc aggtgatatt aatggggctg
     1261 tgatggtagt aaatgcaggg gaaaccgtca attacattta taagcgaaat cttacacata
     1321 agactggtta cagatatttg ctttcttttt ggatctatgt ggtggcaaaa agttcccagt
     1381 tttcgatgga agcaaccaac accacgaccg gaaaaactta tatttacaca ggaccgttgc
     1441 tgacagatga aggcaaatgg attaaatacg atttgaattt ttcggttccc atttcagcca
     1501 atatgactac aaacgaggta actattggtt tacaaaacag aaataaactg ataaccggaa
     1561 atgactatta tatcgatgat atacttgtaa gcacactaca caccgaccca ctcaccattg
     1621 atgtaagcaa caccggtcct gtgtgcgaag gttccccaat tcaacttatt gtcaattccg
     1681 ggggtgatgc tttacccttc acctatgcct ggacaggtcc aaacggattt aactcggcag
     1741 aatcttcccc aatactttca aacagcactg ccaatatgtc cggggtatat aatgtaactg
     1801 taaccgatgc gctggggcaa gtagcaaccg caacaacaaa cgtaattgta aatactgtcc
     1861 caaaaagcga tttttcagtt tcaggaaata ccatagacaa taaaaataat atagtgactt
     1921 tcagcattac acctgaagac ggggtggagt atgaatggga tttaggtgac ggaacgaagg
     1981 aaaaggggtc aacttttact cacacatatt ctattgatgg caacacatac gagtacaaaa
     2041 ttcaattaaa atccactatt tctcagggtt gcagcagcac tacatctaaa acaattgaag
     2101 tcacgccgtt tgtacccaat gtgttttcgc ccaatggtga tggtgtgaac gatcatttta
     2161 tgacaggttt tgatactcaa atatttgacc gctcgggcaa cctgcttttc agtggcactt
     2221 ccggttggga tggtaattat cacggaaggg aaatggataa tgatacttat ttctatcaaa
     2281 tcaaatatac cgataagtac cgggaagtga gtaatttgaa aggatttgtg atattaaaaa
     2341 gataagtggg atatgaaaag acaactatat atcggattac tgctgatagg ttaccttgca
     2401 tcaggtaatc atatcttttc acagaccgat ataagtatgg caactcactg gtacaaccgg
     2461 ggaaattaca atccggcttc cattgcccgt actgattata tttacctgtt ctcaaatgtt
     2521 cgaagccagt ggaccggaat aaccggagct ccacgagtgg ccaatgttca ggtttccggc
     2581 tatttcaatt acatcaattc ggcttttgga ttatcggcag tgaatgaccg cattggtatt
     2641 acaaatgcac ttaaccccat gttgaattat gcttaccggc tatcaaatga tcagtcctgg
     2701 tcattagcgt ttggagtggg actcggggca tatatccgca ccattgatat cagttcttat
     2761 gcacccgtta ccgatcttga tccggcactc atgcacgatg tagcaccgca aacaaagccg
     2821 gatgccaata ttggggttga atttcaaaat gcctcgttca ttttcggcct ttcatccact
     2881 catttatttt cggttttcaa agacagcacc ctgtttctca atgcaaatca tcggtatggt
     2941 tatgccatat ataaaaatac aaagtccgaa gtgctgaatt attacctggg tgtgcaggtg
     3001 gttaaccgat ccaatatttt tgtggtggaa gggaatggca tcgttcgttt caagcaccca
     3061 acaggattat cgaccggcag tcgtgaaatc tttgatctag gtctcaccta ccgaagcacc
     3121 aagcaactga ctgctttatt gggagtaaat ctaaccgaaa atttcagagt agggtatgct
     3181 tatgaccaga gttttatcac tggttataat cagaatggca gtcatgaaat catgctcgaa
     3241 taccggattc catacctgaa tgcagtatgc aaaacctgcc gcgaccaaca actatggtac
     3301 agataacctt aacgtcgttt tatcagcatt aatttctgct aaaaaacggt tagaaaagtg
     3361 tttttacgaa catttcacaa cctgatttgt ttcaattttc actaaaataa gattatggat
     3421 aaaaagaaat ttgatgtggt ggggatgact tgctcggctt gtgtggcaca cgtggagaaa
     3481 agtgtacgga aacttaacgg ggtgcaggag gtaaatgtca atctgcttac gaatagtatg
     3541 acggttgatt atgatcagca agttttatct cctgaggaca tcattttatc ggtggaagat
     3601 gccggatatg aagcatttga aaaagaacaa acaaccgcca aagctaccac accgggtatt
     3661 aatcatgcac tggaagaaca aaaggagatg aaaaaaaggg tgattaattc gtttttgttt
     3721 cttgttccgg tattatatct ttccatgggc agcatggcag gaattccaat tccgtctttt
     3781 ttcgttggtg agcaaaatgc cctcactttt gctttcactc agttgctgct cactattccg
     3841 gtttacttca ttaaccgtaa atttttcaca cacggctttc gctccctgtg gcaactgtca
     3901 cctactatgg acgcgcttgt agccatcggt tcatcggcag cgttgctgta cggcattttt
     3961 gccatttatc gaatttctta cggacttggt caccagcaat tgcaccttgt tcatacctat
     4021 gctcatgata ttttcttcga atcgggtgct acgatactca cattaattac cctggggaaa
     4081 tattttgaag caaaatcaaa gcgtcagact tccgaagcac tgacaaagct gattaattct
     4141 gctccacaga cggccaatgt catccgcaaa ggaaatgaaa ttgaaattcc cgttgaggag
     4201 gtgaggctga atgatattgt cagcattcgg ccgggtgaaa aaatcgcagt tgacggcata
     4261 gtcgaaagcg gttcgggctc ggttgaccaa tcggcactca ccggtgaaag cattccggta
     4321 ttcaaacaga caggtgaacc agttctgtcg gccagtatta acaaaacagg ttattttact
     4381 ttccgtgcaa caaaggtcgg gaaagatact acactttctc agattattca attattggag
     4441 gaagcatcgg catccaaagc tcccatttca aaactcgccg accgcatcag ccggatattt
     4501 gtacccgttg tgattggaat tgcattgttg gcaactatcg tttggctgct attaggttac
     4561 cctttcgaat ttgcattatc cataggaatt gctgttctga ttatttcctg tccgtgtgcg
     4621 ctggggctgg ctacaccggt tgctatcatg gtgggtaccg gaaagggagc tgaaaatggt
     4681 gtacttttca aatcggcaga agccctggaa atgggtgaca aaatagacac aataatattt
     4741 gacaaaaccg gcacgctcac cgaaggaaaa ccgaaagtga ccaatgtgga agcattacat
     4801 tatttaaatg aagaagaatt actgaaaatt agtgcatcac tggaacgata ttccgaacat
     4861 ccggtctcgg aggctatttt gcaaaaagca aaagaattaa agtatgctct ttacgaagtt
     4921 acggcatttg aaaatactcc cggtaaagga atttccggaa aaatcaacgg tcaggtttac
     4981 aaaataggga atttgagttt tatttcggaa aatactaaca cagaagatat ggattatctt
     5041 atccatcagt atgctcaaag tggaaaaaca ccccttatca tcaccgacga tgagaatata
     5101 ctgggaatta tagcggtggc agataccgtt aaaccaacaa gcagagaggc cgtaactttg
     5161 ctcaaaaata tgaacctgaa aaccatcatg ctgaccggtg ataacaaacg gacagcgttg
     5221 gctattcagg aagaactggg tctggatgaa gtaatggcag aagtaatgcc gcaggacaag
     5281 gataaaatag tgcaacaact tcaaaccgaa gggaaaaaag tagcgatggt cggcgatggt
     5341 atcaatgact ccccggcgtt gatgcgtgct gatctgggat ttgccattgg ctccggaacg
     5401 gatattgcaa ttgatgctgc cgatgtggtg ctgatgaaaa gtgatttgct ggatgtggtt
     5461 accggcatcc ggttgagtaa agcggtaatt aaaaacatca aagaaaacct gttctgggca
     5521 tttttttaca atataatcgg catcccgctg gccgcaggag tgttttatac ggcactggga
     5581 tggaaactta gtcccatgtt tgcagccgca gccatgagcc tgagctcggt aacagttgtg
     5641 atgaatgcac tacgattgaa aaacttcaaa cccacaaaat cacatgtaat caattaatta
     5701 tcaattatta taatatttat attatggaaa aaactgcaat gattaaagga atgagctgct
     5761 cacactgtgc agcccgcgtg gaaaaagcgc tgaataaaat agatggagtg gaagccaggg
     5821 tggatctgga aagtcgctct gctaaactta tactgacgaa agatgtatcg gataaaacaa
     5881 taaaagaagc cgttgaggcc atagactacg aagtcaccga tatattagaa taagcaaaaa
     5941 aacgggtcat ttgccggccg gttttctggc cttatttgat aaaaataaaa aataaatacc
     6001 tgaaaatttg gctggtacct gtttgttttt gttattttgc acccgatttc gaattcgtaa
     6061 ataaaaatt
//
LOCUS       DAOPZK010000118         6032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_16250_length_6032_cov_25.813452, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000118 DAOPZK010000000
VERSION     DAOPZK010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16250_length_6032_cov_25.813452"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            717..>1855
                     /locus_tag="PKH68_07300"
     CDS             717..>1855
                     /locus_tag="PKH68_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HNX12781.1"
                     /translation="MKKHLLATLTILTAATWVAAQPLSNMKPYSIEKDRLLYTVGYAH
                     LDTEWNWDYPTTINELILNTMTENFRLFEKYPDYVFNFTGSRRYNMMKEYYPELFRKV
                     QQYVAQDRWHVSGSSVDEGEVNISSSESLVRQILYGNQFFRTEFGKESVDYMLPDCFG
                     FLSNLPTVFNHCGLLGFSTQKLTWRSAAGIPFNVGVWNGPDGKGIVAALNATDYGGRI
                     EKRLDKNDRWAARLDDNKQKSGYAFDYRYYGVGDEGGAPRETDVKNALASQHNGDENF
                     RVLLTSSDQMYRDITPAIRAKLPTYAGELLLIEHSAGSMTSQAFMKRMNRKNELLAQS
                     AEMAAAYADLSGSNPYPYGKLNSAWDLVLGSQFHDILPGTSIPKA"
     assembly_gap    1856..1955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1956..3605
                     /locus_tag="PKH68_07305"
     CDS             <1956..3605
                     /locus_tag="PKH68_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNX12782.1"
                     /translation="RELLKSPARLEFLKESPQQWAAWNMDWKDRQQAAVDFMDKDATI
                     TVVENGPVRVTLEVRRKGHGSEIVQQYSLSAGEAGKRVEITNKIDWQSRGVSLKASFP
                     LAVSNEKATYNLGVSTISRGNNNEKQFEVPSKEWFDLTDKSGKYGVSILEDCKFGSDK
                     PDDNTLRLTLLYTPAVNWNWLTYQSTQDWGIHDVKYAVYGHKGNPNEGGSAWQAKFFN
                     QPLLAFEVPKHAGENGKSFSMISISNPNAGLMAFKKAEKGDYYIVRFNELSGTDQKTV
                     KVKFPAAVADAYEVNGQEKRIAAATVKNNELVFDLSHYTLRSFAVKLKSPVRNAEPLQ
                     AEIDLPYNSDFISSDSNRWDTSNSQSYPAELLPETVISENIKFRIGNKTDEQLNVVNC
                     TGQTIQLPAGNYTSVYVLGAALRDKTADFILDGKKMPVSFGAWNGYVGQFYNRILSRD
                     KNTVVEMEKPYVKTDNIAWFASHCHNSYPMKNLAYQYCYLFKYEIPVPAGAKTITLPD
                     DKDIYIFAMTAATSPAEGVKSLQPLYDNFAGNPDFRLRSGK"
     gene            4309..>6032
                     /gene="typA"
                     /locus_tag="PKH68_07310"
     CDS             4309..>6032
                     /gene="typA"
                     /locus_tag="PKH68_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNX12783.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMLVAGHLFRNNEQTGELIMDN
                     NDLERERGITILAKNVSIDYEGYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKALALGLKPIVVVNKVDKPNCRPDEVNEMVFDLMYHLNATEDQLDFK
                     TIYGSAKNNWMADDWHQPGEDIRPLLDAIIEYIPEPIMLEGTPQMLITSLDYSSYVGR
                     IAVGRVHRGTLKEGMDVMLCKRDGSRAKTRIKELHTFTGLGRSKTNEVKSGDICALVG
                     IDGFEIGDTIADLQNPEPMKPIAIDEPTMSMVFTNNDSPFYGKEGKFVTSRHIHERLV
                     KELDKNLALRVEKEVNADVWHVFGRGVLHLSVLIETMRREGYELQVGQPQAIFKVIDG
                     VRCEPIEQLTINLPEECSGKIIEMVANRKGEMISMEALSDRIQLEFTIPSRGIIGLRN
                     NVLTASAGEAIMSHRFLEYQPYKGEISRRTNGSIIALETGTAFAYALDKLQDRGKFFI
                     FPQDQVYAGQVVGENSKEGDIVVNVTKSKKLTNMRASGADDKSHLAPPIVFSLEEALE
                     YIKEDEYV"
BASE COUNT         1777 a         1367 c         1352 g         1436 t
ORIGIN      
        1 gttcccggcg gtatttagta ataccatcta atcattcaat atatactgca gtgaagaatt
       61 tatcttaatc aaaatcaatg atgcaattat tatccggaaa ttttccgcac agcgtgattt
      121 tttcaatatt atcccgtttt cgttgataat aattgttttt accaacaaag cgcagagcaa
      181 aacacacggc tcatatgacc ggttcaaatg aagatttgaa accgggggaa actcgttcat
      241 gttccttgta gcccatgatt atagagctat caattgacaa tcgagattaa atttacaggt
      301 atttaactta tacagaaatg taaacaaacg gttggatagt gtgaataact aaacaactga
      361 gatataaaca aacaggatga gaaacaaatt ggcaaaacaa aagtaataat aatattttca
      421 ataaaaaatt tttatttgaa taaaattaaa aattatacac aaatattttg attttgttct
      481 gatacgctgg taatcagggt ttgcgcccgg tcaaccgatg gatggtttta ccggtttgtt
      541 aaagacaaaa ggatgtcatt tgttttccga aaacgaaaaa tacggatgat ctcctgataa
      601 attcaccggg ataaacttgc gcatcaaagg aaattgttat ttttgtaaaa aatatgcatt
      661 ccctcaactc ttaccaaaac accggttaaa caccttttaa ttctcctgat atgaacatga
      721 aaaaacacct tttggccaca ctgacaattc ttacagcagc aacatgggtc gccgctcagc
      781 cactgagcaa catgaaaccc tactccatcg aaaaagaccg gctgctctac accgtaggct
      841 atgcgcacct ggataccgaa tggaactggg actaccccac caccatcaac gagcttattc
      901 tcaatacgat gaccgaaaac ttccggctgt tcgaaaaata cccggattat gtgttcaatt
      961 tcaccggttc gcggcggtac aacatgatga aggaatatta ccccgaactg ttccgaaaag
     1021 tgcagcagta cgtggcacag gacaggtggc atgtgtcggg ctcgtcggtg gatgaagggg
     1081 aagtgaatat ctcctcgtcg gaatcgctgg tgcgtcagat actgtatggc aatcagtttt
     1141 tccggacaga attcggcaaa gaaagtgttg actacatgct ccccgactgt ttcggatttc
     1201 tgagcaacct gcccacggtg ttcaaccact gcggactgct gggtttctcc acccaaaaac
     1261 tcacctggcg atcggctgcg ggcattcctt tcaatgtagg ggtgtggaac ggacccgacg
     1321 ggaaaggcat tgttgccgca cttaatgcca ccgattacgg cggaagaata gaaaaaagat
     1381 tagataaaaa cgatcgctgg gctgcccggc tcgatgataa caaacaaaaa tcgggctatg
     1441 cgttcgatta ccgctattac ggtgtgggcg atgaaggcgg cgctccgcgc gaaaccgatg
     1501 tgaaaaatgc gctggccagt cagcacaacg gggatgaaaa cttccgcgtg ctgctcactt
     1561 cgtccgatca gatgtaccgc gacatcactc ccgccatccg cgcaaaactg cccacttatg
     1621 ccggtgaatt attattgata gagcacagcg cgggttccat gacttcgcag gcatttatga
     1681 aacggatgaa ccgcaaaaat gaactgctgg cccaatcggc cgaaatggca gctgcctatg
     1741 ccgacctgtc gggcagcaat ccctatccgt acggcaaact gaacagtgcc tgggatttgg
     1801 tactgggaag tcagttccac gatatcctgc cgggcaccag catcccgaaa gcgtannnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggga actgctgaaa agtccggcac
     1981 gactggagtt cctgaaggaa agtccgcaac agtgggctgc ctggaacatg gactggaaag
     2041 atcgtcagca ggcagcagta gatttcatgg ataaggacgc aaccatcacg gtggtggaaa
     2101 acggtccggt tcgtgtcacc ctcgaagtgc gccgcaaagg tcacggctcg gaaattgtgc
     2161 agcagtacag cttatcggca ggcgaagcag gcaaacgggt ggaaattacc aataaaatcg
     2221 actggcagtc gcgcggagtg agtctcaagg catcgttccc gcttgctgta agcaacgaaa
     2281 aagcaaccta caacctgggc gtaagcacca tcagccgcgg caacaacaac gagaaacagt
     2341 ttgaagtgcc gtcgaaggaa tggtttgacc tgacggataa aagcggaaaa tacggagttt
     2401 ctattctgga ggattgcaag ttcggttccg acaaacccga cgacaacacc ctgcgcctga
     2461 ccctgctcta cacacccgcc gtcaactgga actggcttac ctatcagagc acgcaggact
     2521 ggggaattca cgatgtgaaa tatgctgtat acggacataa aggcaacccg aatgaaggag
     2581 gatctgcctg gcaggctaaa ttcttcaatc aaccgctcct ggctttcgaa gtacccaaac
     2641 atgcgggaga aaacggcaaa tcattctcca tgatttccat cagcaacccc aatgcgggat
     2701 tgatggcttt caagaaagcc gaaaaagggg attattacat tgtccgtttc aatgaattat
     2761 cgggcacgga tcagaaaacg gtgaaagtga aattccccgc agccgtagcc gatgcttacg
     2821 aagtgaacgg acaggaaaaa aggattgcag ctgccaccgt gaaaaacaac gaactcgtgt
     2881 ttgacctgag ccactataca ctccggagtt ttgccgtgaa actgaaatca ccggtacgaa
     2941 atgcagaacc cttgcaggct gaaattgatc ttccttacaa ctcggacttc atcagctccg
     3001 actccaaccg ctgggatact tccaactcgc aatcgtatcc ggccgaattg ctgcccgaaa
     3061 cggtgatcag tgagaatatt aaatttagaa taggcaacaa aaccgatgaa caactgaatg
     3121 tggtgaactg caccggacaa accatccaac ttccggcagg taattacacc agcgtctatg
     3181 tattgggtgc tgcactgaga gataaaacgg ctgatttcat cctcgacggg aaaaagatgc
     3241 ccgtaagttt cggtgcctgg aacggctatg tgggtcagtt ctacaaccgg atactgagtc
     3301 gcgacaaaaa tacagtggtg gaaatggaaa aaccgtatgt aaagacagat aatattgcct
     3361 ggtttgcttc ccattgccac aattcatatc cgatgaaaaa tctggcttac cagtattgct
     3421 atctgtttaa atacgaaatt cctgttccgg ccggcgcaaa gaccattact ctgcccgatg
     3481 ataaggatat ttacattttt gccatgacgg cagcgacatc accggccgaa ggtgtaaaat
     3541 cccttcagcc gctgtacgat aatttcgccg gtaatccgga tttcaggctg cgcagcggga
     3601 aataaatttc agtaataata agagagaaat gctgaataca tagctatttc cgcatttctt
     3661 cgtttaaaat acctactgat attttataca aaattactgt tgtccgtaaa aattcaaaat
     3721 tcttgaaatt taattgattt ctcaactcgt tcgaaatgaa atagttaaaa cactattttt
     3781 agatccctca cttcacgttg ttgcgtatcg ggatgacagt caatcgtttt agcaaatacc
     3841 gctgcccgac tgttgtttgc aatatcatct ttgatgtggc agcggttcga gagacttttc
     3901 gaacccgtcg caaaaagaat ttgattaacc acgtaaacct tcggtgacgg gtttcttaat
     3961 agaaaattca ctgtcatccc gactgtaagg agggatctaa ctattcatgt gatctccata
     4021 ttatttccta ctaatatttt gttgtacaat atcggaataa tagtcagtat tttttttgga
     4081 atgggattag ctccgttctt atacaagcca gcccatccct ccattaatca ccataactcc
     4141 gtttcagaca gatcacagcg aaggaactaa gtattttaaa gaataacagt gatacaaaat
     4201 gaaatcatgg atatcatttt ctgatattta acgcatagta atacagccct atctgcaaaa
     4261 atttcaggga ttttcactac ctttgtgccc tcaaataaaa aggtatacat gcaaaagatt
     4321 agaaatattg ccattatcgc tcacgtcgac catggtaaaa caactttagt tgacaaaatg
     4381 ctggttgccg gtcatctgtt tcgcaacaac gaacagaccg gtgaactgat tatggacaat
     4441 aatgatctgg aacgcgaacg gggcatcact attctggcca aaaacgtttc gatcgattac
     4501 gaaggatata aaataaatat cattgatact ccgggacacg ccgactttgg cggtgaagtg
     4561 gagcgggtac ttaacatggc cgatggcgtg ctgctgctgg tggatgcttt cgaaggaccc
     4621 atgccgcaaa cccgttttgt gctccagaaa gcacttgctt tgggattgaa acccatcgtc
     4681 gtagtcaata aagtggataa gcccaattgc cgtcccgacg aggtgaacga aatggttttc
     4741 gaccttatgt atcatctcaa tgccaccgaa gatcagctcg atttcaaaac catctacgga
     4801 tcggccaaaa acaactggat ggccgatgac tggcaccaac ccggcgaaga catccgaccg
     4861 ctgctggatg ccattattga atacattccc gaacctataa tgctggaagg aactccgcaa
     4921 atgctcatca catcactcga ttattcgtcc tatgtaggcc gtatagcggt aggaagagtt
     4981 caccggggca cattgaaaga aggaatggat gtaatgctct gtaaaagaga cggatcaaga
     5041 gctaaaacaa gaattaaaga acttcacacc tttaccggcc tgggacgttc gaaaaccaat
     5101 gaagtcaaat cgggcgatat ttgcgctttg gtgggtatcg atggtttcga aatcggcgat
     5161 accattgccg acttacaaaa tcccgaaccg atgaaaccga ttgccattga tgaacctacc
     5221 atgagtatgg ttttcaccaa caacgattcg cctttctacg gcaaggaggg taaatttgta
     5281 acctcgcgtc acatccacga gcgactggtc aaggaactgg ataaaaacct ggctttacgg
     5341 gtagaaaaag aggtaaatgc agacgtttgg catgtgtttg gacgtggtgt gctgcacctt
     5401 tcagtgctca ttgaaaccat gcgccgtgaa ggctacgaac tgcaggtggg tcagccacag
     5461 gccatcttca aggtaattga cggagtaaga tgcgaaccga tagaacagct caccatcaac
     5521 ctgcccgaag aatgctcggg aaaaatcatt gagatggtgg ccaaccggaa gggtgaaatg
     5581 atcagcatgg aagctctctc cgaccgtatt caactggaat tcaccattcc ctcgcgcgga
     5641 attatcggac tacgcaacaa tgtgctgacc gcatcagcgg gtgaagccat catgtcgcac
     5701 cgttttctgg aatatcagcc ttacaaaggc gaaatctcac gtcgaaccaa cggttcaatt
     5761 attgctctcg aaaccggaac tgcctttgcc tatgcgctcg acaagttgca ggatcgtgga
     5821 aaattcttta ttttccccca ggatcaggtc tatgccggcc aggtagtggg cgaaaattca
     5881 aaagaaggtg acatagtagt caacgttacc aaatcgaaaa agctgaccaa catgcgtgcc
     5941 tccggtgccg acgataagtc acacctggct ccgcccattg ttttcagcct cgaagaagca
     6001 ctggaatata tcaaggaaga tgaatatgtg ga
//
LOCUS       DAOPZK010000119         5909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_16727_length_5909_cov_23.924838, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000119 DAOPZK010000000
VERSION     DAOPZK010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16727_length_5909_cov_23.924838"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1349)
                     /gene="der"
                     /locus_tag="PKH68_07315"
     CDS             complement(36..1349)
                     /gene="der"
                     /locus_tag="PKH68_07315"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNX12784.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLTGSRRAIVNEEAGTTRDRQYGK
                     SEWAGREFSVIDTGGWVVNSNDVFEEEIKRHVLIAMEEADVILFLVDVQSGVTDLDIH
                     VAQILRKTDKPVILVSNKTDTFDLQYQSAEFYKLGLGDPFMLSAINGLGSGELLDELL
                     KHFKPDTSEEIDDELPRFAVVGRPNAGKSSLINAFMGEDRTIVTDIPGTTRDSIYSRF
                     NKYGHDFYLVDTAGIRKKAKVNEDLEYYSVVRSIRAIENSDVCILMLDATRGLESQDL
                     NIFSLIQKNKKGLVVCVNKWDLIENKESNDIKILEKGIRERISPFVDFPIIFTSVTTK
                     QRILKVLDTAVRVYENKKRKIPTARLNEFFLPLIENYPPPALKGKYIKIKYVTQLPGT
                     YIPSFVFFANLPQYVKEPYRRFLENKIRANWDFHGTPINIFIRQK"
     gene            1655..2374
                     /locus_tag="PKH68_07320"
     CDS             1655..2374
                     /locus_tag="PKH68_07320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNX12785.1"
                     /translation="MILIAASLLPVVLLMAYFYYRDKFEKEPVKVLMKAFGAGILSVF
                     PAVLLATLMSVPNIETYSPAYSSFIRAFWEAALPEEICKFALLYLFIWRDRNFNEYYD
                     GIIYAVFVSLGFAGVENIMYVVGEGIDVALTRGLLSVPLHALCGVIMGYYFSLAKFNT
                     ARQKSYLLKAVAGAIIAHGLYDFILFYTDSLVGISTAFAGLGFLLVFVFIIYLWRFSL
                     RKIRLHVDNSAFKNQANEDEV"
     gene            complement(2427..5642)
                     /locus_tag="PKH68_07325"
     CDS             complement(2427..5642)
                     /locus_tag="PKH68_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX12786.1"
                     /translation="MRKCTLTLFMAIIAVVSITAQQQQNRYSEITNPKLTSINKLPAR
                     SSFFSFTNADEAQKATYSSKGSDYLLLNGTWKFNFTENFSQRPMDDFFREDFNASAWK
                     DINVPGNWEVQGFGYPIYVNTTYEFTSPGHKPFWDRPNPPLVPEEFNATGTYRREFDI
                     PQSWIGKEIILSADAAKGAAYYYLNGSFIGMNKEGKLPARIDITDKVKTGKNVLAVQF
                     HRFSDANYLECQDFWRLSGFDRDVYVYARPKAHIADFFAHTPLDATYTNGKFALDVSL
                     EDKEQTKDEFSVSYTLKDAAGKITATETKSVIINGNMPVTFSKEIPTVKKWSAEEPNL
                     YTLCIELKDKTGKTLEATAVKVGFRTAEIKNKQFLINGQPVLVKGVNIHEHNEFTGHY
                     VTEELMRKDFELFRKYNVNTARTCHYPQSELFYKLADEYGIYVIDEANIEAHGMGYDL
                     RKGATLANNPLFLEAHLTRTSEMVERDKNHACVVTWSLGNESGNGYNMYATYNLIKNR
                     DTSRPVQYERAGLEWNTDIFCPMYDRPEEIENYAKHPNSDRPLILCEYAHAMGNSLGN
                     FIDYWDIIRKYPLLQGGCIWDWVDQGLAQKDAEGKPFWAFGGDFGPTGTPSSGDFNCN
                     GLVLPDRTTKPHTEEMRKVYQNVWFKNFNAQKETVDIYNENFFIDLSQYNLFYTIKSN
                     GKVLKTGQIAANVKPQETKTVIIPGISKYFNAKNQVTINFEVKQKADTRYIPAGWIVA
                     RDQFVVNEYAKLALNKKAPATVTQNTTNLIFNGSDFSVVFDKASGQMTSYKYKGIEYV
                     SDRFGLKPFFWRASTDNDYGAGLPRKLKAWREASYIALKAENLQVKNGENTSISCEYN
                     FPQTKTTWKVNYTIYKDGAIRVENSVNATACDLPLIPRIGMRMQLPGEFVNAEYYGRG
                     PWANYEDRKTSTFIDRYKSPIINMVDKYVMTQENGHHTDASWLAVTQQSGKGLAFIAE
                     GKFQFNVSNYLLETVANAEDWNNDAPVGTAPKNKHYNEYRPSNKVDLFIDYRMMGVGG
                     NNSWGEWPMLPYRIIPKDTNISYSFTIVPIDNTASIDKMLK"
BASE COUNT         1487 a         1224 c         1273 g         1925 t
ORIGIN      
        1 gttttgtttt aggtggttga caactaattc aatgactatt tctgccggat aaatatatta
       61 atgggtgtgc cgtgaaaatc ccagtttgcc cgtatcttat tttccagaaa acggcgatag
      121 ggttctttca catattgtgg cagattggca aaaaatacaa atgatggaat atatgtaccg
      181 ggcaattggg ttacatattt tattttgatg tattttccct tcaaagcggg aggcggataa
      241 ttctctatca gcggcaggaa aaattcattc agccgggctg ttggtatttt tcgtttttta
      301 ttctcataaa ccctcaccgc tgtatcgagc actttcagga ttcgttgttt ggtggtaact
      361 gaggtgaaaa tgattggaaa atcgacaaaa ggcgaaattc gttcgcggat acccttctcc
      421 agtatcttga tatcgtttga ttctttattc tcaatcaaat cccacttatt cacacagact
      481 accagtccct ttttattctt ttgtatcagg gaaaagatat tgaggtcctg actttcgaga
      541 ccacgggtag catcgagcat caggatacac acatccgagt tttcgatggc acgtatggaa
      601 cgcactacgg aataatactc cagatcttcg tttaccttgg ctttcttgcg gataccggct
      661 gtatctacca gataaaaatc gtgtccgtat ttattgaacc gcgagtaaat gctgtcgcgc
      721 gttgtgccgg gtatatcggt tacgattgtt cgatcttcac ccatgaatgc attgatcaat
      781 gaggattttc cggcattggg acgtcccacc acagcaaacc ggggtaattc atcatctatc
      841 tcctcgctgg tgtcgggttt gaaatgtttg agcaattcat cgagcaattc tccgctaccc
      901 agcccgttga tggccgaaag cataaagggg tcgcccagcc cgagtttgta aaactctgct
      961 gactgatatt ggagatcaaa tgtatctgtt ttgtttgaaa cgaggatgac aggcttgtcg
     1021 gttttacgaa gtatctgcgc tacgtgaata tcaagatcgg ttacgccgct ttgaacatcc
     1081 accaggaaca aaatcacatc tgcttcctcc atggcgatta aaacgtgacg ctttatttct
     1141 tcttcaaata catcattgga gttaactacc catccaccgg tatctataac cgaaaattcg
     1201 cgtccggccc attcgctttt gccatactgg cggtcgcggg tagtaccggc ttcttcattt
     1261 acgatagccc ggcggctgcc ggtaagtctg ttgaaaagtg tggacttgcc cacattgggg
     1321 cgtcctacta ttgctacaat atttcccatt gtttttttgt ttaagtcccg gggaattacc
     1381 cggaacggtt taactttcat ttttatttgc tgctgtccgt ttcgtcgcga aacagtacag
     1441 tgggtacaaa tattacggct gcaaaggtac tacaattttc cggataaatt ctgactgtcg
     1501 ataaactgtt ttaattgagg tatttaaata tcgatttttt caaatatcat tcgattcgga
     1561 tatgaggttt cttcattttt ttctttaatg aatttaatga atatccgttt tattaaacaa
     1621 aatactgtat ttttacccct taaaaacatt gaatatgata ctgattgctg cgtccctgct
     1681 tcccgttgtg ctgcttatgg cttattttta ctaccgcgac aagttcgaaa aagaacctgt
     1741 aaaagtttta atgaaggcat tcggagcggg tatattatcg gttttcccgg cggttttgct
     1801 ggctacactg atgtcagtac ccaacattga aacttattca ccagcttaca gttcatttat
     1861 tcgggcattt tgggaagcag ccttacccga ggaaatctgc aaatttgcac tgctttatct
     1921 ttttatctgg cgcgatcgca atttcaatga atattacgac ggaataattt atgctgtgtt
     1981 cgtatctctg ggatttgccg gcgtcgagaa tatcatgtat gtagtgggcg aaggcattga
     2041 cgtagctctt acccgaggat tgctttcggt acccctgcat gctttgtgcg gtgtgattat
     2101 gggttactat ttttcgctgg caaagtttaa cactgcccgt caaaagagtt acctgctgaa
     2161 agccgttgcc ggcgcaataa ttgcacacgg gttgtatgat ttcatacttt tttataccga
     2221 tagtctggtg ggtatcagta ctgcatttgc cggattgggt tttcttttag tttttgtatt
     2281 tattatctat ctctggcgat tttccctccg aaagatacgt ttacatgtcg acaattcggc
     2341 attcaaaaac caagccaatg aggatgaggt ttaaaactaa aaaagggatg ttcgcatccc
     2401 ttttttagtt ttaaatttta agtttgttat ttcagcattt tatcgattga agcagtattg
     2461 tcaatcggca ctatggtgaa actgtatgag atattcgtgt ctttgggtat aatccggtaa
     2521 ggcagcatgg gccattctcc ccagctgtta ttgccaccaa cacccatcat gcggtaatca
     2581 atgaaaagat ccactttgtt ggatggtctg tattcgttgt aatgcttatt tttaggtgcc
     2641 gtacccaccg gagcatcatt gttccagtct tcggcgttgg caactgtttc cagcaaataa
     2701 ttggacacat tgaattgaaa cttaccttca gcaatgaacg caagtccttt acctgattgc
     2761 tgtgttacgg ccagccaact ggcatcggtg tggtgtccgt tttcctgagt cattacatat
     2821 ttatccacca tattgataat gggtgattta tagcggtcga taaatgtgga tgtttttcgg
     2881 tcttcgtagt tagcccatgg tccacgaccg tagtactcgg cattgacaaa ctcaccgggg
     2941 agttgcatac gcatacctat gcgcggaatg agtggcaagt cacatgctgt agcattcacc
     3001 gaattttcca cacgaatagc accatctttg taaatcgtat agttcacttt ccaggtggtt
     3061 tttgtttgcg ggaagttata ttcacagctt atcgaagtat tttctccgtt tttcacttgc
     3121 agattttcag cttttagcgc gatgtaactt gcttcccgcc acgcttttaa ttttcgtggc
     3181 aaacctgcac cataatcatt atcggtagag gctctccaga agaaaggttt caggccgaac
     3241 cggtcgctta cgtactctat acctttgtat ttgtaggatg tcatctgtcc gcttgctttg
     3301 tcaaaaacaa ccgaaaaatc ggagccgttg aaaataaggt tggtggtgtt ttgagttacc
     3361 gtggcgggtg cttttttatt cagagccaat ttagcgtatt catttaccac aaactgatca
     3421 cgcgcaacaa tccaaccggc aggaatgtag cgtgtatcgg ctttctgctt aacctcaaaa
     3481 ttaatggtaa cctgattttt cgcattaaaa tatttagaaa tcccgggaat tattaccgtt
     3541 ttggtttcct gtggtttcac attggctgca atctgccctg ttttcagcac tttgccattc
     3601 gatttgatgg tgtaaaatag attgtactgg ctcaggtcta tgaaaaagtt ttcgttataa
     3661 atatcgacag tttctttttg tgcattgaag tttttgaacc atacattctg atagactttt
     3721 cgcatttctt cggtgtgagg ttttgtggta cgatcaggga gtaccaatcc attgcaatta
     3781 aaatcgcccg atgatggtgt tcccgtgggt ccgaagtcgc ctccgaatgc ccagaatggt
     3841 tttccttcgg catctttctg tgccagtccc tgatctaccc aatcccagat gcagccaccc
     3901 tgcagcaacg gatatttccg gatgatatcc caatagtcta taaaattacc cagactgttg
     3961 cccatggcgt gggcatattc acacaaaatg agcgggcggt ctgaatttgg atgtttggcg
     4021 taattttcaa tttcttcggg tctgtcatac atcgggcaga aaatatcggt attccattcc
     4081 agtccggcac gttcatactg aacaggacgt gatgtatcgc ggttttttat caagttgtaa
     4141 gttgcgtaca tgttgtagcc attgcccgat tcattaccga gcgaccaggt taccacgcag
     4201 gcgtggtttt tatcacgttc taccatttcg gatgtacggg tcaaatgagc ttcgagaaaa
     4261 agtggattat tggctagtgt agctcctttg cggaggtcgt agcccatgcc gtgtgcttcg
     4321 atatttgctt catcgattac gtatattccg tattcatcag cgagtttgta aaacaattcc
     4381 gattgaggat aatggcaggt gcgtgcagta tttacattgt atttccggaa cagttcgaaa
     4441 tcttttcgca tcaattcttc ggtcacgtaa tgccctgtaa attcgttgtg ttcatgaata
     4501 ttaaccccct tcaccagtac cggctgaccg ttaatcaaga attgtttgtt tttaatttca
     4561 gcggttcgga atccaacttt aacggctgta gcttcgagcg tttttccggt tttgtctttg
     4621 agttcaatac acaacgtata aagattaggt tcttcagccg accatttttt tacagtcggg
     4681 atttcttttg agaatgttac aggcatattt ccattgataa ttacagattt tgtttccgtg
     4741 gctgtaattt taccggctgc atctttcagt gtataactaa ccgaaaattc gtctttcgtt
     4801 tgttccttgt cttccaaact cacgtccagc gcaaacttgc cgttggtata agtggcatcc
     4861 agtggagtgt gtgcgaagaa atcggctatg tgagctttgg ggcgcgcata cacatacaca
     4921 tcgcgatcga aacccgaaag cctccagaag tcctgacatt ccaggtaatt ggcatccgag
     4981 aacctgtgaa attgaactgc aagcacattt ttaccggttt tgactttatc ggtaatatct
     5041 attctggcag gaagttttcc ttccttattc attccaataa aggagccgtt gaggtagtaa
     5101 taggcagcac ctttggcagc atcggcgctc agtattattt ctttgccaat ccatgattgg
     5161 ggaatgtcaa attcgcggcg ataggttcct gtagcattaa actcttcggg taccaaaggc
     5221 ggattgggtc tgtcccagaa aggtttatgt cccggtgatg taaattcgta ggtagtattt
     5281 acgtagattg gataaccaaa tccctgaact tcccagttgc ccggtacatt gatgtctttc
     5341 caagcagaag cattgaaatc ttccctgaag aaatcatcca tgggtcgttg actgaagttt
     5401 tcggtaaagt tgaatttcca ggttccgttc aacagcagat aatcacttcc tttggaagaa
     5461 tacgtggctt tttgagcttc atcagcattt gtaaaagaaa aaaagctact gcgagcgggc
     5521 agtttgttga tactcgtcag tttcgggttg gtaatttcgc tgtagcggtt ttgttgctgt
     5581 tgcgcagtga tacttaccac agcgataata gccataaaca gcgttagagt gcattttctc
     5641 atggtttttg aatttatatt aaagttaaac ttattgcgtc agacaaagat acataatatt
     5701 tttgtaatgg gcgaacagga aaaaaataca atttcttgct tccggaaatt caactgataa
     5761 tctgacattt accatataca aaagaaaatt tcacagagaa atttgccaat ttagcttgga
     5821 ataattttgt gaaatgtatt aaaatctcta tctttgcacc tctaaaaaat aaggacgaga
     5881 tagctcaggc gggttagagc gtcccgata
//
LOCUS       DAOPZK010000120         5865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_16911_length_5865_cov_24.116351, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000120 DAOPZK010000000
VERSION     DAOPZK010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16911_length_5865_cov_24.116351"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1495)
                     /locus_tag="PKH68_07330"
     CDS             complement(8..1495)
                     /locus_tag="PKH68_07330"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HNX12787.1"
                     /translation="MIIIDLLKQDWKKGFRAKGFYKSLSVKLLMGFMGLYFAAIFLFV
                     GFFLGDMLDEASETLKPLEVFNGATLYILLAALTIRFFMQQLNTINLPAFQSLPVKRS
                     TLVNFLLLKPAFSFGNYMTLLVVIPFAVKSVFAYYSGAIALRFVLNCILLIWFDMWMA
                     AYLKRRFGSSLLTLPVILLVLGGLAALEYFKVFSLFDVSRQIFDVLIINPFGLLLTAA
                     GAGLAYVLNLWFFSRNYYPEKFNEKLKLNDSRVTGGFSFLDRFGITGELISLQIRLML
                     RHKRTKTLLYMSAFFLVYGLLFYTNPVYADQPGWLFFCAMFMTGILMLMYGQWIFSWE
                     SSYFDSILTKNIPIKTYMNANYYLLVAFNVITFVLTTPYFFFGPKIILLHIAAFLFNT
                     GVNIFLLIFFGSFNTKRVDLNARSSFNYQGTTYKSFLIILPIMFLPMIIVGVLSWLVS
                     TNVALIVLSVLGLMGIIFKEPLMKLSINQFNKRKYLMAAGFRDKA"
     gene            complement(1705..4539)
                     /locus_tag="PKH68_07335"
     CDS             complement(1705..4539)
                     /locus_tag="PKH68_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNX12788.1"
                     /translation="MKRIYLFVAALFIATFSSFAQQQMPPIPVDKDVRIGKLDNGLTY
                     YIRKNNLPENQANFYIVQKVGSMQEEENQRGLAHFLEHMAFNGSKNFPNDSKGKGIIP
                     YLETVGVKFGQNLNAGTSFDYTVYNIDAVPTAKQAVLDSCLLILHDWSGFLLLRDKDI
                     DKERKVIHEEWRTRQNMSMRMLGVLLPEIYKGSKYADRLPIGKMEVIDNFEPKVLRDY
                     YQEWYRPDLQGIIIVGDVDVDKTEQQIKSMFADIKKPVNPAERVYSQVPDTDEPIVAI
                     ATDKENPNTNVLVFYKRNIVPADKKMNMDYLVYDFMNSMIEQMLSSRMQEMIQKENPP
                     FAFAEVSSGSYLISKTKDAMQFFAVSNPNATNLAIETIIREANRAKEFGFTAGEYERA
                     KADYMSKLEKLYNERDKQRSSYYIDQYTSNFLENEPIPGIADKYNTIQQVVNYIPLEA
                     INQLVKGMITDKNRVVAIMGTEKENYSPEADIIALIKKVDSEKITAYVDNAITEPLMA
                     AMPAKGKIVKEEQNQGATVWTLSNGAKVVVKPTKFKDDEILLSGFAPGGSSVIDNKYI
                     SEIKVLDKIFEAIMGESALSAGGLGKFSKTDLTKVLSGKNVSMNFSIDTYNEAISGKS
                     NVKDLETAMQLLYLNFTDVRKDQSAYTSFATRTKGMLTNFGSNPMIAFSDSIMSSMYK
                     NNPRGRFISAKDVEAADYDTMLKLRKERFANAGSFIFTIVGNVTPEALKPMVEQYIAS
                     LPSTSEKSKFNAKVMPQRKGKFNNNFIRPLEVPKATTGLIYSGKLKYNLQNDIQLDAL
                     EQILDIVLTDKIREKLGGTYGVQISAELEKTPEEGFNIQLMFDTDPERRAELVNAIDV
                     TISEMQKTGPSAENVQKVKEFMTKQHADELKKNDYMLNTLNNQYRYGVDFATNYDQYV
                     DNLSVNSLKKFAKGLFSQGNRIEVSMSSGK"
     gene            complement(4573..4797)
                     /locus_tag="PKH68_07340"
     CDS             complement(4573..4797)
                     /locus_tag="PKH68_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNX12789.1"
                     /translation="MPIIINLDVMMAKRKISLNELSERIGITPTNLSILKTGKAKAIR
                     FSTLEMICKELDCQPGDILEWDGKTSEEEQ"
     gene            complement(4814..5506)
                     /locus_tag="PKH68_07345"
     CDS             complement(4814..5506)
                     /locus_tag="PKH68_07345"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HNX12790.1"
                     /translation="MEFNSKNRRLGVLTVLAIIIYIALILSSFFSGIDDFMLGFEEGS
                     KKSTSMKEGKIQTKNLTTVYFLDLKAKPGYSTFPDSISNMKGNGKIAIRYDKAQAHYN
                     DFTEKVANRSFYDTSQIILSLLTLVIIAMIPVFFIKLNVRLKKELVFDKRNIKNMRCI
                     GVLLLLYYLVSFLQNWVVYSMNIQVFEFRDYVIQFEQADLIWVLLGVVVLLFAEVLSR
                     GSQLKEEQELTI"
BASE COUNT         1772 a         1230 c         1044 g         1819 t
ORIGIN      
        1 agggggctta tgctttatct ctgaaaccgg cagccatcag gtattttcgt ttattgaact
       61 gattgatgga aagtttcatc aaaggttcct taaaaataat ccccatcaat cccaacaccg
      121 aaagtacaat cagtgcaaca ttcgtcgaaa ccaaccagga aagtacaccc acaataatca
      181 tgggcagaaa catgattggc aatattatca gaaaactctt ataggtggtt ccctgatagt
      241 tgaaggagct gcgggcattc agatccaccc gttttgtatt gaaacttccg aaaaatatca
      301 gcaggaagat attgacaccg gtattgaaca ggaaagcggc aatatgcagc aggattattt
      361 taggaccgaa gaaaaaataa ggtgttgtaa ggacaaaagt aatcacatta aatgccacca
      421 gcagataata attagcattc atataggttt taatgggaat atttttagtg agtatgctgt
      481 cgaaatagga gctttcccaa ctaaatatcc actgtccgta catcagcata aggataccgg
      541 tcataaacat ggcacagaag aaaagccatc cgggctgatc ggcatatacc ggattggtat
      601 aaaaaagcaa tccataaacc agaaaaaaag ctgacatata caacagtgtt ttagttcgtt
      661 tgtgtcggag catcagcctt atttgcagtg aaataagttc tcccgtaatg ccaaaccgat
      721 cgaggaagga aaatccacct gtcacccgac tgtcgttcag tttcagtttc tcgttgaatt
      781 tttcgggata ataatttcgg gagaaaaacc acaggttcag cacataagcc aggcctgctc
      841 cggcagctgt cagtagcaaa ccgaatggat ttattatcaa gacatcaaaa atctgacggg
      901 aaacatcaaa cagcgaaaaa accttgaaat actccaaagc agccaatccg ccaagaacaa
      961 gcagaatcac cggaagcgtg aggagcgaag agccaaatcg tcgtttcaga taggctgcca
     1021 tccacatatc gaaccagata agcagaatgc agttcagtac aaaacgaaga gcgattgccc
     1081 cggaataata cgcgaaaacg cttttcacag caaacggaat gacaacaagc agcgtcatgt
     1141 aattgccaaa gctaaacgcc ggtttcaaca gcaggaagtt aaccagcgtg gatcgcttca
     1201 caggcaacga ctgaaaagcg ggcagattaa tcgtgttgag ctgctgcata aaaaaacgga
     1261 tggtaagcgc tgcaagcaga atgtacaggg tagcgccgtt aaatacttcg agcggtttca
     1321 gcgtttcgct tgcttcgtcg agcatatctc ccagaaagaa acccacaaac aaaaagatag
     1381 cggcaaaata caagcccata aaacccatca gcaatttcac tgataaactt ttataaaacc
     1441 ctttcgcccg aaatcctttt ttccagtcct gttttagtaa atcgattata atcatataaa
     1501 tagttgtttc gaagttgcaa aaatataaac aaatctgtca atatcaatca aattgttaaa
     1561 acattcatca tttattattc attgaggcaa ttgcaggcat aaaaccggta acttattatc
     1621 tgtttttcct tttaaataaa aaaagaatag cacagcacaa ttatgcacta tgctattctt
     1681 cactcaatta ataaacaaac aaaatcattt accgctactc atggatactt ctatgcgatt
     1741 accctgactg aataaaccct tagcaaattt cttcaacgaa ttaacagaca aattatcaac
     1801 atattgatca taatttgttg caaaatcaac accgtatctg tattgattat tcagggtatt
     1861 caacatatag tcatttttct ttaattcatc ggcatgctgc ttagtcataa attcttttac
     1921 tttctgaaca ttttcagcac ttgggcctgt tttctgcatt tcgctgattg ttacatctat
     1981 cgcgtttacc aattcggctc tgcgttcagg atctgtatca aacatcaact ggatgttaaa
     2041 tccttcttca ggagtttttt ccaactctgc cgatatctgc acgccatacg ttccgcccag
     2101 tttttcacgg attttatcgg taagtacaat atcaaggatt tgctccaaag catccagctg
     2161 aatatcgttt tgaagattgt atttcagttt tccggaataa atcaaccccg tggtagcttt
     2221 tggaacttcc agcggccgga taaagttatt attgaactta ccctttcttt gaggcatcac
     2281 cttcgcgtta aatttactct tttcagaagt ggaaggcaag gaagcaatgt attgttccac
     2341 catcggcttc aatgcttcgg gcgttacatt accaacaatc gtaaagataa agctgccggc
     2401 atttgcaaag cgttctttgc gcagtttcag catggtatca taatcggctg cttccacatc
     2461 ctttgcagaa ataaatcgtc cgcgcgggtt gtttttatac atagaactca taatggaatc
     2521 ggaaaaggct atcatcgggt tgctgccgaa attggtcagc atgcctttag tgcgggttgc
     2581 aaaggatgta taggcggact gatctttacg aacatcggtg aagttcagat aaagtaactg
     2641 catagccgtt tcaagatctt ttacgttcga tttacctgaa attgcttcgt tataggtatc
     2701 aatgctgaaa ttcatgctta cgtttttacc tgacaagact ttcgttaaat cggttttgct
     2761 gaatttaccc aaccctccgg ccgacaaagc agattcgccc atgatggctt caaagatttt
     2821 atccagcact tttatctctg aaatatattt attgtcgata acagagctgc cgcccggtgc
     2881 aaaacctgac agtaagattt cgtcatcttt aaatttagtt ggttttacca ccacttttgc
     2941 accgttggaa agtgtccata cggttgcccc ctggttttgt tcttctttta ctatttttcc
     3001 ttttgcaggc attgctgcca tcagcggttc agtaatagca ttatccacat aagcggttat
     3061 tttttcactg tccacttttt tgatcaatgc aataatatcg gcttcgggtg aataattttc
     3121 cttttcggtt cccataattg ctaccacgcg gtttttatcg gtaatcattc ccttaaccag
     3181 ctgattgatg gcttccagcg gaatataatt taccacctgc tgtatcgtat tgtatttatc
     3241 ggcaatgccg ggaatcggct cgttttcaag aaaattactg gtgtactgat caatgtaata
     3301 actgctgcgt tgtttatcgc gttcgttata taatttttcc agcttactca tgtaatcggc
     3361 tttagcccgt tcatattctc cggccgtaaa accgaattct ttggcgcggt ttgcctcacg
     3421 aattatcgtt tcgatagcca ggttggttgc attggggtta ctcaccgcaa aaaactgcat
     3481 tgcgtccttg gttttggata tcagatacga gccactgctt acctcagcaa aagcaaaagg
     3541 aggattttct ttttgtatca tttcctgcat acggcttgac agcatttgtt caatcatact
     3601 gttcataaag tcgtaaacaa gataatccat attcattttt ttgtccgccg gcacaatatt
     3661 gcgtttataa aacaccagaa catttgtatt tggattttcc ttgtcggtag caatagccac
     3721 gataggttca tccgtatcgg gaacctgaga ataaacacgt tctgcaggat taaccggttt
     3781 ctttatatcg gcaaacatgc ttttaatttg ttgctcggtt ttgtccacat ccacatctcc
     3841 aacgataatg attccctgta aatcggggcg ataccattcc tgataataat ctctcagtac
     3901 tttcggttca aaattatcta tcacttccat ttttccaatc ggcaggcgat cggcatattt
     3961 gctgccttta taaatttcag gtaacaaaac acccaacatg cgcatggaca tattctgacg
     4021 ggtacgccat tcttcgtgaa ttactttgcg ctctttatca atatctttat cgcgaagcaa
     4081 taaaaagcct gaccagtcgt gtaaaataag aaggcaagag tccaaaacgg cttgtttggc
     4141 tgttggcact gcatcaatat tataaacggt atagtcaaaa ctggtcccgg cattcaggtt
     4201 ttgtccgaat ttcacaccga cagtttccag atagggaatt attcctttcc ctttgctgtc
     4261 attcgggaaa tttttggaac cgttaaaagc catatgttcc aggaagtgag ccagaccacg
     4321 ctgattttct tcttcctgca tggaccccac tttctgtaca atgtagaagt tagcctgatt
     4381 ttcaggtaaa ttgtttttac ggatgtaata agtcagtccg ttgtcaagtt ttcctatccg
     4441 tacgtcttta tcaaccggaa taggcggcat ttgctgttgt gcaaatgagc tgaacgtagc
     4501 gataaacaat gctgcgacaa ataaataaat tcttttcatt ttttaagttg taattattta
     4561 gtttgtaaaa atctattgtt cttcttctga tgtttttcca tcccattcca gaatatcacc
     4621 cggctggcag tctagttctt tgcaaatcat ttccagcgta ctgaaacgaa tggcttttgc
     4681 ctttccggtt ttcagaatgg aaagattggt tggcgtaatt cctatacgct ccgaaagttc
     4741 attcaatgaa atctttcgtt tagccatcat cacatctaaa tttattataa tcggcatatt
     4801 tttttctctc ttcttaaatc gttaattcct gctcttcttt caactgcgaa ccacgtgaca
     4861 atacttccgc aaataaaagt acaaccactc caagcaacac ccagattaaa tcagcttgtt
     4921 caaactgaat aacataatcc ctgaattcga atacctgtat gttcatgcta taaaccaccc
     4981 agttctgaag gaacgaaacc agataataca gcagaagcaa aacaccaata catcgcatat
     5041 tcttaatatt tcttttatca aatacaagtt ctttcttcag cctgacgttt agttttataa
     5101 aaaacacggg tatcattgca ataattacaa gcgtaagcaa tgaaagaatt atctgagatg
     5161 tgtcataaaa cgaacgatta gctacttttt cggtgaaatc attataatga gcctgcgctt
     5221 tgtcataacg gattgctatt ttcccgtttc ctttcatatt actgatacta tcagggaaag
     5281 tcgagtatcc gggctttgcc tttaaatcaa gaaaataaac agtagtaaga ttttttgttt
     5341 gtattttacc ctccttcata ctagttgact ttttactccc ttcttcaaat cccagcataa
     5401 aatcatctat tcccgaaaaa aacgatgaca aaatcagcgc aatataaatg ataattgcta
     5461 aaactgtcaa aacgcctaat cttctgtttt tagaattaaa ttccataaat atctgttttt
     5521 aatctgtttt caatgaaatg tatttttacg atgcaaaaat aacacactat ttccgataaa
     5581 caaaatatat ttatcgttta tcgatatatt ttcgtcttaa aacattcaat acaagattta
     5641 gaatattata aaatcgggtt agaaaagtac tgtaaagcaa tattccggca accaaaaata
     5701 ttgtgttaaa tattctgaat attattttca aggtaaatct gacttaacca aaaaaaaaca
     5761 tctcatccgc gcgaagcaaa tgagatgttt ttattattag atgtttagcc ggagtttatt
     5821 tttcttcttc tttgtaccaa ccggcataca tggcgtaatt gtgcg
//
LOCUS       DAOPZK010000121         5734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_17501_length_5734_cov_24.214474, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000121 DAOPZK010000000
VERSION     DAOPZK010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17501_length_5734_cov_24.214474"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..719
                     /gene="rpe"
                     /locus_tag="PKH68_07350"
     CDS             72..719
                     /gene="rpe"
                     /locus_tag="PKH68_07350"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNX12791.1"
                     /translation="MTKIISPSLLAADFSNLQSACEMINASEAQWLHIDVMDGVFVPN
                     ISFGFPVLEAVKTHCTKPLDVHLMIIEPEKYIERFVEAGANILTVHLETLKNPITTLR
                     QIREMGVKAGLTINPDIPVESLRSLVSEADMVLLMSVFAGYGGQKFIEETYSRIEELK
                     AIIADENPECLIEIDGGVNTENAPVLFAAGVNVLVAGNSVFRSPDPIATIHRMLG"
     gene            738..2816
                     /locus_tag="PKH68_07355"
     CDS             738..2816
                     /locus_tag="PKH68_07355"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNX12792.1"
                     /translation="MDFLLRTPFFRLLLAIIPGIVLFQYVQLPPSALYITGGISSVFV
                     LISFLIRKSNFQFKFRWLFGVSMTIFLSALSYVLCLNFEKNHTFSSLNEHAVCEVELE
                     AAPVEKAKSYLCKVELVQKYDSTGNENTYGHAIIYLQKDSSVRQLLLGDRLLVDAEFK
                     SPDGVQNPQGFDYARYLRRQGILATAYVPTGKWQKSKTLPAFSMLHPLKYIYRLAGMS
                     RNYLLNIYRTSGIRGDEFAVLAALTLGFTDELQPDLLKSYSATGAMHILSVSGLHVGI
                     VYAVILFLLGFMNKKKWQRVMRVMLIILFLWAYAFITGLSPAVLRATLMFSFVTVATG
                     MGWKSKIYNNIFASAFLLLLFNPNLIFDIGFQLSYSAVLSIVFFQPQISTWFVTSNKL
                     LKKSWDLVAVSLAAQLGTAPFVLYYFHQFPNFFLLTNLVAIPLSTIIIYLAIALLIIS
                     VVPYLSVAIAFLLNWSLWLLNYLIVGIQNLPWSISTISMDFRQLIFAFIAIAFLSAYF
                     FRKNYTILTAGLVSILLVCGMYSFQKYKSLTTSKFIVFADSRVPIINFVERGRNYVFT
                     TDTAQSEKIAGAFWKSNLLKSPEYIDNTSWFNDGFASFHNKRFIILKDTLLKNKTTNQ
                     PLQVDYLVLSNRVKPKMEQILDCIQPMMVVADKSISKWYTSNARKVCESRGITFWSVA
                     EKGAFVVNFK"
     gene            2947..3969
                     /locus_tag="PKH68_07360"
     CDS             2947..3969
                     /locus_tag="PKH68_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNX12793.1"
                     /translation="MISKIIGEDLVHRTKIEIENADNIVIITHVGPDGDAMGASLGLW
                     HFLMTLEKTPQVIVPTPFPNFLAWMPGANKTLVYKFDKEKADEHIQKADLIFLLDFNA
                     ASRMDKMADAVLASKARKVMIDHHLQPENIANIIISYPEISSTSELIFRLICRMGHFS
                     DINLGCAECVYTGMMTDTGGFTYNSNHEEIYSIIYELIKLGVDKDDIYRRVYNTFSAD
                     RMRLMGYCLYKKMKIYPEYQAALITLTQRELHEFNYDNGDAEGFVNIPLSIQGINFTV
                     FMREDPDKIKVSLRSQGSFPTNKFAAEIFGGGGHLNASGGESYTSLDEAVRKFEEALP
                     LYTEFL"
     gene            4090..5322
                     /locus_tag="PKH68_07365"
     CDS             4090..5322
                     /locus_tag="PKH68_07365"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HNX12794.1"
                     /translation="MKYIIILGDGMADEPIASLGNKTPLQAAATPHIDKLARMGRCGM
                     LDTIPVGFAPGSEIANLSVLGYDLNSVYEGRGVLEAASMGVEIEDNEIAMRCNLICIE
                     NGIIKSHSAGHISTEEAGELIDFLQEKLGDDSVSFYPGVSYRHLLKLKNGNKHLKCTP
                     PHDVPGTPFSSVMIQALTPEAQPTVEFLNKLILKSQTLLENHPVNIRRASQGKEKAIS
                     IWPWSPGNRPKMQKMTDIYPIKSGSVISAVDLIKGIGVYAGLKNIDVEGATGLYTTNY
                     EGKARAAIEALKTDDFVFLHIEASDEAGHEGDVELKIKTIEYLDSRIVKPILEELLTW
                     NEPVTIALLPDHPTPCAIRTHTNTPVPFIIYRTDNVPDKVVVYDEFSCMEGSYGLLKG
                     SEFIEEVIKLPPTPLKGE"
     gene            5415..>5734
                     /locus_tag="PKH68_07370"
     CDS             5415..>5734
                     /locus_tag="PKH68_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNX12795.1"
                     /translation="MNIVSNIEMESSPFGALGVKTEGIKYAGSKKKIIPYIVQIISEL
                     EGVKNVLDGFSGTTRVSQAFAQLGYNTTSNDISAWSEVFATCYLKAGKEDKYYREIIE
                     HLNS"
BASE COUNT         1606 a         1217 c         1249 g         1662 t
ORIGIN      
        1 taattttttc aggattttga aagtttgcaa attttaccgt aagtttgtaa ttcataatca
       61 acatttgacg tatgacaaag attatctccc cttccctcct tgcggcagat ttcagcaatt
      121 tgcagtcggc ctgcgaaatg attaacgcca gcgaagcgca atggctgcat attgatgtga
      181 tggacggggt ttttgtacca aatatttcct tcggttttcc ggtcctggaa gcagtaaaaa
      241 cccattgtac aaagccgctg gatgtgcatc tgatgattat tgaaccggag aaatacatcg
      301 aacggtttgt ggaagccggt gccaatattc tcaccgttca tctcgaaacg ctgaaaaatc
      361 caattactac cctgaggcaa atcagggaaa tgggcgtgaa agccggtctg actatcaatc
      421 ccgatatacc tgttgaaagt ttacgtagtc tggtgagtga agccgacatg gtattgctga
      481 tgtctgtttt tgccggttat ggcgggcaaa agttcattga agaaacctat tcgagaattg
      541 aggaactgaa agccatcatt gccgatgaaa accccgaatg tctgatagaa attgatggtg
      601 gagtaaatac tgaaaatgct cccgtacttt ttgctgcagg tgtgaatgtg ctggttgccg
      661 gtaattcggt attcagatca cccgatccca ttgccaccat tcaccgtatg ctgggctgaa
      721 ataaaaaaag ttgaaatatg gattttctcc tcaggacgcc atttttccgc ttattgctgg
      781 ccataattcc cggcatcgtg ctctttcagt acgttcaact tccaccctct gccctgtaca
      841 ttaccggagg aattagttcg gtcttcgtgc tcatctcttt tctaatcaga aaatctaatt
      901 ttcaattcaa attcaggtgg ttgttcggtg tcagcatgac catttttctt tccgcattga
      961 gctatgtgct gtgtctgaat tttgaaaaaa accacacttt cagttcgctc aatgaacatg
     1021 ctgtctgtga agtggaactg gaagcggctc ccgtggagaa agccaaatcg tacctctgca
     1081 aagttgaatt agttcaaaaa tacgattcca caggcaatga aaacacttat gggcacgcca
     1141 taatttatct gcagaaggat agttcggtga ggcaactctt attgggtgac cgccttcttg
     1201 ttgacgctga attcaaaagc cccgacggtg ttcaaaaccc gcagggattc gattatgccc
     1261 gctacctccg acggcaggga atacttgcga cagcttatgt tcccaccggg aaatggcaga
     1321 aatcgaaaac tttgccggca ttttcaatgc tacacccctt aaaatacatt tatcgcctgg
     1381 ccggtatgag tcgcaactat ttgctgaata tttaccggac gagtggcatc aggggagatg
     1441 aatttgcggt actggcagca cttacgctcg gttttaccga cgaactccag cccgacttgc
     1501 tgaaaagtta cagtgcaacc ggtgctatgc atattctttc ggtcagcgga cttcatgtgg
     1561 gaatagtgta tgctgttatc ctgtttttgc tgggttttat gaataaaaaa aagtggcaga
     1621 gagtgatgag agtgatgctg ataattctgt ttttgtgggc ttatgctttt attaccggac
     1681 tttcgccggc ggtgctgcgt gccactctta tgttcagttt tgttacggta gctaccggaa
     1741 tgggatggaa gtcaaagatt tacaacaata tttttgcttc cgcttttctc ctactccttt
     1801 ttaacccaaa tctgatattt gatattggct tccaactcag ttactcagct gtattgagca
     1861 tcgtgttttt tcagcctcaa atctcaacct ggtttgtcac atccaacaaa ctgctcaaaa
     1921 agtcgtggga tttggttgcc gtttcgctgg ctgcacaact tggtactgca ccttttgttc
     1981 tctattattt tcatcagttc cccaatttct ttctgctgac caatctggta gccatacctc
     2041 tatccaccat tatcatttac ctggctatcg ctttattgat tatttctgtt gttccctatt
     2101 tgtctgtagc aattgcattc ttgctgaact ggtcgctgtg gttgctcaac tatctgattg
     2161 tgggtattca gaatttaccc tggtctatca gcaccatttc tatggatttc cgtcaactta
     2221 tttttgcttt tattgctatc gcattcttat cggcatattt tttcaggaaa aattatacta
     2281 ttctgactgc cggattagtt tccattctgc ttgtatgtgg tatgtattct tttcagaaat
     2341 ataaaagtct gacaaccagc aaatttatag tatttgcaga ttcccgtgtg cctataatca
     2401 actttgtgga aaggggtaga aattatgtat ttactaccga taccgctcaa tctgagaaaa
     2461 ttgcaggggc attctggaaa agcaacctgt tgaaatcgcc cgaatacatc gataatacat
     2521 cgtggttcaa cgatggattt gcaagttttc acaacaaacg ttttataatt ctaaaagaca
     2581 ctcttcttaa gaataaaacc accaatcaac cgctgcaggt ggactatctg gtgctttcaa
     2641 acagggtaaa accgaaaatg gagcaaatac tcgattgtat tcaacccatg atggtagtag
     2701 ccgataaaag tatctcaaaa tggtacacct ccaatgcccg aaaagtatgt gaaagccgtg
     2761 gcatcacatt ttggtctgtg gcagaaaaag gtgcttttgt tgttaatttt aagtgattaa
     2821 cacctacaag ttaggaattt caattatttg ccatgttttt ttgttacttt tgcaaatatt
     2881 tttctgaaaa tgatcaggaa atatctgttg ttttacattc gttcacttac acaaataaaa
     2941 taaaaaatga tatctaaaat cataggcgag gatttggttc acagaaccaa aatcgaaata
     3001 gaaaacgccg ataacatcgt cattatcaca catgtgggtc ctgatggcga tgccatggga
     3061 gcttcactgg gtctctggca cttcctgatg acccttgaaa aaacaccgca ggttatcgta
     3121 cccacaccct ttccaaattt tctggcctgg atgccgggtg ccaataaaac cctggtttac
     3181 aagttcgaca aagaaaaagc agatgaacac atacagaaag cggatctgat ttttttgctt
     3241 gatttcaacg ctgcaagcag aatggataag atggccgatg ccgtattggc ctcaaaagca
     3301 cgaaaagtaa tgattgacca ccacctgcaa cccgaaaata ttgcaaatat catcatctcc
     3361 tatcccgaga tttcatccac cagcgaactc attttcaggc tcatctgccg tatgggacat
     3421 ttctcagaca ttaatctggg atgtgccgaa tgcgtctaca caggtatgat gaccgatacc
     3481 ggtggattta cctataattc caaccacgaa gaaatttatt cgattattta tgaattgata
     3541 aaactgggtg tggataagga tgatatttac cgtcgtgtgt acaatacgtt ttcggccgac
     3601 cggatgcggc tgatgggcta ctgcctctac aaaaaaatga aaatctaccc ggaatatcag
     3661 gctgcactta ttacgcttac ccagcgagag ttgcatgagt ttaactatga caacggcgat
     3721 gctgagggct ttgtaaacat cccattatcc attcagggta ttaatttcac tgtttttatg
     3781 cgcgaagatc cggacaaaat caaagtctcg ctgcgctccc agggaagttt ccccaccaat
     3841 aaatttgcgg ctgaaatttt cggcggcggc ggtcacctga acgcttcggg cggcgaaagc
     3901 tatacctcgc tggatgaagc agtacgaaaa ttcgaggagg cattgccttt atataccgaa
     3961 ttcttataag ataataaatt tccaaacaaa gacttttaag aggtttttat attttctttt
     4021 atctattgta aatcccaaat tgtctttatt aatcaatatt ccccccttta gggggttagg
     4081 gggttcttta tgaaatacat cattatactc ggcgacggta tggctgacga gcctatcgca
     4141 tcacttggca acaaaacacc gcttcaggca gctgctacgc cacacatcga caaactggcc
     4201 cgaatgggac gctgcggaat gctcgatact atacccgtcg gctttgctcc gggtagtgaa
     4261 attgccaatc tttcggtttt aggctatgat ttgaacagcg tgtatgaagg cagaggcgta
     4321 ctggaggctg ccagtatggg cgtggaaatt gaggataacg aaatagccat gcgctgtaat
     4381 ctgatttgca tcgaaaatgg aattatcaaa agtcattcgg ctggacatat cagcaccgaa
     4441 gaagccggtg aactgattga ttttttgcag gaaaaactgg gagatgattc tgtaagtttc
     4501 taccccggag tgtcgtatcg tcatctttta aagttgaaaa acggaaataa acaccttaaa
     4561 tgcacaccgc ctcacgatgt tcccggcact ccgttttcgt cagtgatgat acaggcattg
     4621 accccggaag cgcagccaac agtcgaattt ctgaacaaac tgatacttaa atctcagact
     4681 ttgctcgaga atcatccggt aaatatcagg cgggcaagtc agggcaaaga aaaagccatc
     4741 agcatctggc cctggtcgcc gggcaaccga ccaaagatgc aaaaaatgac cgatatttat
     4801 cctataaaaa gcggttcggt tatctcagcc gtggatctca tcaagggaat tggcgtatat
     4861 gccggactga aaaacataga cgtggagggt gctaccggac tttacacaac caactacgaa
     4921 ggaaaagccc gtgcagccat tgaagcgttg aagaccgacg actttgtgtt tcttcacatc
     4981 gaagcgagcg acgaagccgg gcatgaaggc gatgtggaac taaaaataaa aacgatagaa
     5041 taccttgaca gccgtattgt taagcccatt ctggaagaat tgcttacgtg gaatgagccg
     5101 gttactatcg cgctgctgcc cgaccatccg acgccctgcg ccatacgcac tcacaccaat
     5161 acaccagtgc catttatcat ctatcgtacc gataatgtgc cggataaggt ggtagtgtac
     5221 gatgagtttt cgtgcatgga agggagttac ggcctgctga aaggaagtga atttattgaa
     5281 gaggtgataa aactgccccc taccccccta aagggggaat aagtgctaac aatgagattt
     5341 ttttattaat attagaactt ggttatcaat actttccaaa aatcagcaat aagatatttc
     5401 aattttatat atttatgaac attgtctcta atattgaaat ggaaagttcc ccctttgggg
     5461 cgttaggtgt caaaacagag ggtattaaat acgcgggttc gaaaaagaaa attattccgt
     5521 atatcgttca gattattagc gaattggagg gtgtaaagaa tgtgttggat ggcttcagcg
     5581 gaacgacacg ggtgtcgcag gcgtttgcac aactgggcta caacaccact tccaacgata
     5641 tttcggcctg gagtgaggtt tttgcaacgt gctatttgaa agccggtaaa gaggataagt
     5701 attatcggga aattattgaa cacctgaata gttt
//
LOCUS       DAOPZK010000122         5696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_17668_length_5696_cov_23.927672, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000122 DAOPZK010000000
VERSION     DAOPZK010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17668_length_5696_cov_23.927672"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKH68_07375"
     CDS             complement(<1..845)
                     /locus_tag="PKH68_07375"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNX12796.1"
                     /translation="MAYINDNFIKIPATYLFSEIAKRVAQHKKDNPEADIIRMGIGDV
                     TLPLPDASVDALITAANEQRKSKTFRGYGPEQGYDFLRNSIVENDFKSRGMDIEADEI
                     FVSDGAKSDTGNIGDIFGLQNRVAITDPSYPVYVDTNAMSGRAGEPSASGQWTNLIYL
                     PCNTANNFVPELPKEKVDIIYLCYPNNPTGTTLTKEQLTVWVNYARENNAIILFDAAY
                     EAYITEENVPHSIFEIEGAREVAIEFRSFSKTAGFTGTRCGYTVVPKTVTGLSESGEK
                     VSLNKL"
     gene            complement(955..1821)
                     /gene="dapF"
                     /locus_tag="PKH68_07380"
     CDS             complement(955..1821)
                     /gene="dapF"
                     /locus_tag="PKH68_07380"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNX12797.1"
                     /translation="MKFTKMHGTGNDYIYVNGFEQTIENPSETAIRLSDRHKGIGSDG
                     LVMILPSETCDFRMRMFNSDGSEAEMCGNASRCVGKYVYDKGLTTKTEVTLETLAGVK
                     VLQLFVNNDNKVDKVTVDMGEPILDAKLIPTTFDESPVINKVVSFGKYTDYQVTCVSM
                     GNPHAIIFMDNVSELDLPKIGPVVENAIYFPNRTNTEFVEIISNSVLKMRVWERGAGE
                     TMACGTGACATVVAAVLNGVADRKTTVQLLGGNLEIEWREADNHVYLTGDAVTVFEGE
                     TPNLPKQVRDRL"
     gene            2147..2902
                     /locus_tag="PKH68_07385"
     CDS             2147..2902
                     /locus_tag="PKH68_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12798.1"
                     /translation="MKSNFYKLILTALLALPVFGLIQAQEPIKTDTTTATLKTTTQVE
                     PIGNTFYHWETPVLEPINIDSIRQQKQDYEAYLEQIDSLENVLKSNKKQIKQYQDQAN
                     SEAKLVKKERSNIKQKVNFYKEDEKLLKTEKKLRDKEMKMLRKERRDFVKASKGLVSN
                     DVDYRNMKFNERENRIVDADANWATKMDNLRKNQVQIQSDQKKLEDREREVLTRQTEL
                     KTYDEAIQLKAQQIAVEKKQANLEIKRAKMQLK"
     gene            complement(4042..4472)
                     /locus_tag="PKH68_07390"
                     /pseudo
     CDS             complement(4042..4472)
                     /locus_tag="PKH68_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
     assembly_gap    4473..4572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4573..5660)
                     /locus_tag="PKH68_07395"
     CDS             complement(<4573..5660)
                     /locus_tag="PKH68_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12799.1"
                     /translation="MKNHILTLLIFNFVSVILFAQSSNPLSTYTELPNPKATNTALWA
                     SQPDVSVAWGTTDTRYTKQNPSGLSAANKSISLTAWKGERVSAQFVVSNKTGTNTLSY
                     SFGDFINVNDENLRITSDNVFSGFVRYVMTDELNKDGGGACGYRNAADFDYSLSADPI
                     DHHAKNLEIKPLTSQGVWVRVAVPAGTTAGNYTSTINVKLNNAVVETLQINLKVQNRT
                     LPAATNWKFHLDLWQNPYSVARYYGVVPWSEAHMEALKNEHQLYADAGGKVITASIIY
                     DPWNSQTEDVYGTMIEWIKKKDGSWSFDYTNFDKWVELMMSLGVNKQINCYSMIPWKL
                     SFRYFDEAAGTYKDAVTKPGDALYEEMWT"
BASE COUNT         1607 a         1141 c         1187 g         1661 t
ORIGIN      
        1 agtttgttca gactaacttt ctctccgctt tccgacagac cggtaacagt ttttggcaca
       61 acggtataac cgcagcgggt accggtaaac ccggctgttt tggagaaact tcggaattca
      121 atagccactt ctctggctcc ttcaatttcg aaaatgctgt gaggcacgtt ttcctcggta
      181 atgtaggctt cgtaagcagc atcaaaaaga ataatcgcat tattctcacg ggcgtaattt
      241 acccaaacag tcagttgttc ttttgtaagt gtggttccgg taggattgtt gggataacac
      301 agataaataa tgtccacttt ctctttcgga agttccggaa caaagttatt ggccgtattg
      361 cagggaagat aaataagatt agtccattgt ccgctggcag atggttcgcc tgcgcgaccg
      421 ctcatggcat tggtgtccac atacacggga taacttggat cggtaatagc cacgcgattt
      481 tgcaatccga aaatatcccc gatattgccg gtatcgcttt tagctccatc gctcacaaag
      541 atttcgtcgg cttcaatatc cattccgcga gacttgaaat cattttcaac aatgctgttt
      601 cggagaaaat cgtaaccctg ttccggtccg taccctctga atgtcttact tttgcgctgt
      661 tcgttggcag cagtgatcaa agcatctacc gaagcatcgg gcaggggaag tgttacatcg
      721 cctattccca tgcggataat atctgcttcg ggattatctt ttttgtgctg agctactcgt
      781 tttgcgattt cggaaaatag ataggttgcc ggtattttaa tgaaattgtc gttaatatat
      841 gccattggtt agtttattaa agacccctta cccctaaagg gggataagag aagttagttt
      901 attttgtttt aatgcgactt aaaaattaag agacgaaact aatttaattc tcctttagag
      961 cctgtcccga acttgtttcg ggaggttagg ggtctctcct tcgaagacgg ttaccgcgtc
     1021 gccggtcaga taaacgtgat tgtctgcttc tctccattca atttccagat tgcctccgag
     1081 caattgaact gttgttttgc ggtcggccac tccgttgagt acagcggcaa caaccgttgc
     1141 acaggctccg gtgccacaag ccattgtttc accggctccg cgttcccaaa cccgcatttt
     1201 caatacgctg tttgatatta tttctacaaa ttcggtgttg gtacgattgg ggaaataaat
     1261 ggcattttca accaccggtc cgattttcgg cagatcgagt tcgctgacat tgtccatgaa
     1321 aatgatagcg tgcggattac ccatcgaaac acaggtaacc tgataatcgg tatattttcc
     1381 aaacgaaacc actttgttga tcaccggtga ctcgtcgaat gtggtgggga tgagtttggc
     1441 atcaagaata ggttcaccca tatctacggt tactttatcc accttgttat cattgttcac
     1501 aaagagttga agcactttaa ctccggccag tgtttccagg gtaacttcag ttttggtagt
     1561 caagcctttg tcatatacat attttcccac acagcgcgat gcattgccgc acatttcggc
     1621 ttccgaaccg tcggaattga acatgcgcat gcggaaatca caggtttcgg acggaagtat
     1681 catcaccaga ccatcagacc cgataccttt gtggcggtcg ctcagacgga tggctgtttc
     1741 ggacggattt tctatcgttt gttcgaaacc gtttacataa atgtagtcat tgcctgtgcc
     1801 gtgcattttt gtaaatttca taacgctata attttgaatt tagagcacaa aaatagttac
     1861 attttgccaa aatcgacatc taatgaacaa taaattatta taatttgtag catttgtgtc
     1921 taattgtaag tatatgggtt ctgattaaaa taaaaataat gtaaacaggc ttgtatgtaa
     1981 tcaaaatgaa aaaatcaaat cgcaggcagg attattattt ttaggtgaaa ccatttacag
     2041 ttttacataa aaagctactg taagttataa aaatgtacac tttttttttg taattgacag
     2101 aaaatcgtat atttgtagtt acaaactctc aaaaaattat aaaattatga aatcaaattt
     2161 ctacaagtta attttaaccg cactcttagc acttcccgtt tttggattaa tacaagctca
     2221 ggaacctatc aaaactgata ctactactgc tacgctcaaa accacgacac aggttgaacc
     2281 gatcggcaac accttttatc attgggaaac tcccgtactc gaaccgataa atatagactc
     2341 catccgtcag caaaaacagg attacgaagc ctacctggag caaattgatt cactggagaa
     2401 tgttcttaaa agcaataaaa agcaaatcaa acaatatcag gatcaggcaa actccgaagc
     2461 taagctggta aagaaagaac gcagcaacat caaacagaaa gtaaatttct ataaggaaga
     2521 tgaaaaactg ctgaagacag aaaaaaaact gcgtgacaaa gaaatgaaaa tgctgagaaa
     2581 ggaacgcagg gattttgtaa aagcctcgaa gggactggtc agcaatgatg tggattaccg
     2641 gaatatgaaa ttcaacgaaa gggaaaaccg gattgtggat gccgatgcaa actgggcaac
     2701 taaaatggat aacctccgca aaaatcaggt tcagatacag agtgaccaga aaaaactcga
     2761 agacagagaa cgcgaggtac ttacaaggca gactgaactg aaaacttatg acgaagctat
     2821 acagttaaaa gctcagcaaa tagccgttga gaaaaaacaa gccaacctgg agattaaaag
     2881 agcaaaaatg caattgaaat aatgagattg tctaacagaa atcatttcaa atcgaaaaaa
     2941 aaacttcctg ccaaccggcg ggaagttttt tttactcttc ttgataaata aatgaaattt
     3001 ctttgtcctt tctctttagg gaaatgcagc tacgtcggag ttgacttcta caaagtcagc
     3061 catcataaca ggaatttgtc cggaaaatat aataaaagtc gggaaacttt cttttggagt
     3121 gaaaatgtat ggaaaattcc gggggagaat atgtctgaat tagcgaacag ccaatttgta
     3181 ggcttggttg ggcgttttta cgatgataat tcccgtgggt aaatactgat ggttcattaa
     3241 ttcctgacct gctatattat aaacttttag cgggatagtt gggtcagcgg acactaagca
     3301 attatttatc actttgaaat gctggctgta ggtctgtgtg gcaggaatgt cggttgtgat
     3361 cttttcctga cgaatgatat atttacagcc ggtatcattc aactcagagc cacctcccca
     3421 gttatatatt ttatatccca atcccgaatt agtttgattt atctgcacgt tgccggaact
     3481 gatgataaaa tacttaccct ctcccgtggg tgtgaacttc caacctccgg aggtaataaa
     3541 agtgctgctt gtagaaagtg gattgttaaa agcggcagca ttggagatgt tgctgttacc
     3601 gtttttactt acaatcgcat aagtcccgtc attcaattcc attactttcc acagaaattt
     3661 atcattgttg atactaaagg tgttgccata cagtacggca ttatcgccct gataagcaat
     3721 aaatctgttt tcgcgcaacg gggtgtgaaa tgaataccag acttgttgac tatcattcga
     3781 agctttgggt acattctttg attttttgta ggtttccaac gcgttccaaa gcgatattct
     3841 ttcagtttga aattcggtgt cagttgttcc ggtattcctt acagtaatag cattagcaat
     3901 ggaattactc agaatggatg tgttctcagc ggaatattca cccgggtttt cacctgtcgg
     3961 agctgaattc ataaaaccgg tagcgacttc aatcatcgaa gtcagcatgg atttttcatt
     4021 catttcggct tcactcagtg tgttgatgac atttcttgca tttgaaatga ccgttgctgc
     4081 cggtgtctga cttagttttg attcatcaaa tgcagcaagc gcatcgttga tttgcaatat
     4141 cgctgtctga tttcctgctt gtgtaaataa ttctttcagt attcttattt tttcaaattg
     4201 ctgaatacct tctgtcattc tttcaaatct catagaagta cgtgcgcccg gataaatcag
     4261 ataagtgtca cctgctcccc aggaacggaa cctgctgtca gtcagcggat ttttcaccca
     4321 gcagttgagt gcccagcgga gataaccatc cagattggct tttgctgcgt accaggcaaa
     4381 ccattcacac tctgccggtg aagagaatgt aaatgtattg ggacgagctt cttcgcagga
     4441 agtatagaag gtggtgactt ttccctcagc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nngtccacat ttcttcataa agtgcatcgc ccggtttggt gacagcatct
     4621 ttatatgtac cggctgcttc atcaaaatag cggaatgata gtttccacgg gatcatggaa
     4681 tagcagttga tttgtttgtt aacgccaagt gacatcatca gttccaccca tttgtcaaaa
     4741 ttggtataat caaatgacca gcttccgtct tttttcttta tccattctat cattgttccg
     4801 taaacgtctt cggtctggct attccagggg tcataaatga tagatgccgt aataactttt
     4861 cctcctgcat cagcataaag ttggtgctcg tttttcaaag cttccatatg tgcctcagac
     4921 cagggtacta caccgtaata acgggcaacc gaatatggat tttgccataa gtccaggtga
     4981 aatttccaat tggttgcagc aggcaatgtc cggttttgaa ccttcagatt gatttgaagc
     5041 gtttctacaa ctgcattatt cagttttacg ttgatagtgg aagtataatt accggctgta
     5101 gtgcctgcgg gaacagcaac ccgcacccac acaccctgcg atgttaacgg ttttatttca
     5161 aggtttttag catggtgatc aatcgggtca gcagacagtg aatagtcgaa gtcagccgca
     5221 ttgcggtaac cgcaggctcc tccgccgtct ttattgagtt cgtctgtcat cacataacgc
     5281 acaaaaccgg aaaaaacatt atcggaagtg atacgcagat tttcatcatt tacgttgatg
     5341 aaatcaccga aagagtagct gagggtattt gtacctgttt tgttcgaaac tacaaactgg
     5401 gcagaaaccc gctcaccttt ccaagctgtg agtgagatgg atttatttgc tgctgaaagt
     5461 ccggaaggat tttgtttagt ataacgcgtg tcagtagttc cccaggcaac tgaaacatca
     5521 ggctgcgatg cccacaatgc cgtgtttgtt gctttcgggt tgggaagttc ggtgtaagtg
     5581 ctaaggggat tactgctttg agcaaacagt attacggata caaaattgaa aataagcaag
     5641 gtgaggatgt gattcttcat ttgagaaatg tgatttgaat tagattttca ggaaaa
//
LOCUS       DAOPZK010000123         5683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_17731_length_5683_cov_21.952203, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000123 DAOPZK010000000
VERSION     DAOPZK010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17731_length_5683_cov_21.952203"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKH68_07400"
     CDS             <1..300
                     /locus_tag="PKH68_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNX12800.1"
                     /translation="VGMPGRALEGKFLENAKLGLTRPKSCPYNCLHTCDYKVVPYCII
                     LALYNAYKGKMDKGYAFAGSNAYLSARISTVKEVIHSMISEFNKREADVMAGSNV"
     gene            complement(388..2571)
                     /gene="pnp"
                     /locus_tag="PKH68_07405"
     CDS             complement(388..2571)
                     /gene="pnp"
                     /locus_tag="PKH68_07405"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNX12801.1"
                     /translation="MNVVTKTITLGDGREITIETGKLAKQADGSVVVRMGNTMLLATV
                     CAAQDAVPGTDFMPLSVEYKEKYGAAGRFPGGFTRREGRAGDYEILVSRLVDRALRPL
                     FPDDFHAEVYVNVMLISADGVDMPDALAGLAASAALAVSDVPFHGPISEVRIGRVNGE
                     YIVNPTFEQLASADIDIIVAATLENIMMVEGEMSEVSEDELLTAMKVAHEAIKIQCQA
                     QIELMEAAGKTEKRTYCHEENDEELKKDIWEKTYDKVYAEASSCNTDKHLRAKNFKSI
                     VEEYVAGIDPEVAAAKKELIGRYYHSVEKEAMRRSILDEGKRLDGRKTNEIRPIWSEV
                     DYLPGAHGSAVFTRGETQSLTTVTLGTKMDEKLIDEVLTQGKDRFLLHYNFPPFSTGE
                     ARASRGVGRREVGHGNLACRALKSMIPDGYPYVIRIVSDILESNGSSSMATVCAGTLS
                     LMDAGVQMKKPVSGIAMGLISEKNATNYAILSDILGDEDHLGDMDFKVTGTRDGITAT
                     QMDIKCDGLSFEILEKALNQAKEGRLHILDKIQETLAEPRPDFKPNAPRIVVMMIPKE
                     LIGAVIGPGGKIIQNMQAETGAVISIEEIGDQGRIEIAATNKASIDAAVAKIKGIVAI
                     PEIGETYPSKIKSIMPYGAFVEFMPGKEGLLHISELDWKRIETMDEAGLKEGDMIDVK
                     LLDIDQKTGKFKLSRKALLPRPPREEKPHDDKPREHRGPRPERRD"
     gene            complement(2787..3800)
                     /locus_tag="PKH68_07410"
     CDS             complement(2787..3800)
                     /locus_tag="PKH68_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12802.1"
                     /translation="MKKLISFMVLAVTVLVAVSLFSGCNTYNYGTTNEQTYYENPEWA
                     PEYYPGTRYYYFPDIECYYDLATREYIFLNRGQWFFTPTLLGFYNDFDPMYGFVIIVN
                     TRIYQPWMHHQYYISHYPRYYYRDYYDYSNIPYVRGFNENAKSAVYWPENQRERARDW
                     NDNNIRENRRFKYSPSDRQMQVNTTNRINNQRTAGVTSSRTSGTSGRINDEINTQPRN
                     TNTTVPRGTTTRTATTENTNTRNATTDNVTNQTVNRSTTTTTSRTANTQTDTPVRQSG
                     TVTRSGGRTANTNYYGKPIGQPVRVERQMRSTTPETKTEEGTTTGTPATTRSSETPTR
                     SSR"
     gene            complement(3973..5469)
                     /locus_tag="PKH68_07415"
     CDS             complement(3973..5469)
                     /locus_tag="PKH68_07415"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNX12803.1"
                     /translation="MKLKFSYFLLAFTAFLLSSCDPENVMKPIEKDTKLLFEWDDAKT
                     QTITFSTASISSSSAKVIITGSTATITGGGYYSVSGQMTNGQLIVDAKDQVVKIQFNG
                     ITMNNDTTSPLFIRDAKKTIIFLKTGTTNTFTDGAAYTHVGDPNAAIFSKDYLGFTGD
                     GNLVVNGNYNDAISSDDMLYIDNGNFSVKSIDDGIRGKDSLIVNNGNINVICSTGHAL
                     KSDNVLKPGKGYLKIAGGTLSLTSSDGDGIHTSKRLIIEGGNITARSLNSQALRSDSL
                     VHISGGTVQINASKKGIESPFITISGGTTSIISTKAGVNATYGFGGITTDGSILSISN
                     GNLYINSDDGTGIDSSGDITMSGGTVVVQGPSDKDKEGINYNGKFTISGGFLIASGPQ
                     SILSVKTPGTESSQNSVKLTSSTMATNLINIQDASGKSIVTFKPIRSAYGLLLSSPDL
                     KTGDYNVFKGGTYNGGTTVNGYSKDGTYSNGTLVKTFTVTDKVATVSF"
BASE COUNT         1434 a         1181 c         1262 g         1806 t
ORIGIN      
        1 gtaggaatgc ccggtcgggc attggaagga aaatttcttg aaaatgcaaa actgggacta
       61 acaagaccca aatcatgtcc ctacaattgc cttcatacct gcgattacaa ggtagttccc
      121 tattgcatta ttcttgcact ctacaatgca tacaagggta aaatggataa aggatatgca
      181 tttgccggaa gcaatgccta tctttcggca cgtatttcta cagtgaaaga agtcatacat
      241 tctatgattt ctgagtttaa taaaagggaa gccgatgtaa tggcaggatc gaatgtttag
      301 acaacgccga ataaaaagaa aagaggctgt cccaaaattg aatttgagac agcctctgat
      361 tttttatacg attacaatcg ttcgttttta gtcacgtctt tcaggacggg gtcctctgtg
      421 ttcgcgtggt ttgtcgtcgt gtggtttttc ttcgcgtgga ggacgaggaa gcaatgcttt
      481 acgtgaaagt ttgaattttc cggttttctg atcaatatcc agcaacttca cgtcaatcat
      541 atcgccttct ttcagaccgg cttcgtccat agtctcaata cgtttccaat ccagttcgga
      601 gatatgaagc aaaccttctt ttccgggcat aaattcaacg aatgcgccat aaggcatgat
      661 tgatttaatc ttggatggat aagtttctcc gatttcggga atagctacga tgcctttgat
      721 tttggcaaca gctgcatcaa tagatgcttt gtttgtagct gcaatttcta ttcttccctg
      781 atctccgatt tcttcgatgg agataacggc accggtttca gcctgcatgt tttggatgat
      841 ttttccacca gggccaatta ctgcgccaat caattctttc ggtatcatca tcaccacgat
      901 gcggggtgca tttggcttga aatccggacg cggttcagcc agcgtttcct ggattttgtc
      961 cagaatgtgc agacgacctt ctttggcctg gttcaatgct ttttccagaa tttcgaagga
     1021 gagaccatca catttgatat ccatttgagt agctgtgata ccatcgcggg ttccggttac
     1081 cttgaagtcc atatctccca ggtgatcctc atcgcccaga atgtcggaaa gtatggcgta
     1141 attggttgca tttttttcgg aaatcaatcc catggcaata cctgataccg gttttttcat
     1201 ttgaactccg gcatccatca gcgatagcgt accagcgcaa acagtagcca tggaagatga
     1261 accgttggat tccaaaatat ccgaaacaat acggattaca tacggataac cgtcgggtat
     1321 catcgatttc agtgcacgac aggccaggtt accgtgacca acttcgcggc gaccaacacc
     1381 acgcgatgct cttgcttcac cggtagagaa ggggggaaaa ttatagtgta acaggaaacg
     1441 gtctttgcct tgtgtcagca cttcatcaat cagtttttca tccatttttg ttccgagagt
     1501 aacggttgtc agcgactgag tttcaccgcg ggtgaaaaca gctgaaccat gagctccggg
     1561 cagataatcc acttccgacc agattggacg gatttcattg gttttgcgac catccagacg
     1621 tttgccttca tccagaattg aacggcgcat cgcttccttt tctaccgagt ggtagtaacg
     1681 gcctatcagt tcttttttgg ctgctgctac ttcagggtca attccggcta catattcttc
     1741 aactattgat ttgaagtttt tggcacgcag gtgtttgtcg gtgttgcacg aagaggcttc
     1801 ggcatagacc ttatcgtagg ttttttccca aatatctttt ttcagttctt catcattctc
     1861 ttcatgacag taagttcttt tttcggtttt accggcagct tccatcagtt caatttgtgc
     1921 ctgacactga attttgatag cttcgtgagc tactttcatc gcagtaagca gttcatcttc
     1981 gcttacttca ctcatttcgc cttctaccat catgatgttt tcaagcgttg cagccacgat
     2041 aatatcgatg tctgcactgg caagctgttc gaaagtagga ttaacgatat attcgccatt
     2101 cacacgaccg atacgaactt cggaaatcgg gccatggaaa ggaacatcgg aaactgcgag
     2161 ggcagctgaa gcagccagac cggctaatgc atcgggcata tccacaccgt cggctgaaat
     2221 gagcattaca ttcacataaa cttcagcatg aaaatcatcg gggaacaggg gacgcagtgc
     2281 acggtcaacg aggcgtgaaa ccaggatttc ataatctccg gcacggcctt cacgacgggt
     2341 aaatccaccg gggaaacgtc cggcggctcc gtatttttct ttgtattcta ccgaaagggg
     2401 cataaaatcg gtaccgggaa cagcatcctg tgctgcacat acggtagcca ggagcatggt
     2461 gttacccatg cgtactacca cagaaccatc ggcttgtttg gccagtttgc cggtttcgat
     2521 ggtaatttct ctaccatcac cgagtgtaat ggtctttgta actacattca taaaaattac
     2581 agtttattgt ttttatactt ttttatttga cggcaaaggt acaaattata ttttgatatt
     2641 cagtttgttg acttcagttg ttagttgaaa aaaccttaaa aagaggcttt ccctgtcatt
     2701 ctatcggtta gaatgtttga taaaacaaaa aaagcgggaa gttgaaaatc ccgctttttt
     2761 tttcattatt tttttgcaaa atcaaattat ctggacgatc tggttggcgt ttcgctgctg
     2821 cgggtagtag caggggtacc tgtagtagtg ccttcttctg ttttggtttc gggtgtagtg
     2881 ctccgcatct ggcgttctac tcttaccggc tgaccgatgg gtttgccgta ataatttgta
     2941 ttggcagtgc gcccccccga ccgtgtaacc gtacccgact gacgaacggg agtatcggtt
     3001 tgagtattag cagtacgtga ggttgtagtg gttgtacttc tgtttacggt ttggtttgtt
     3061 acattatcag tagttgcatt tctggtattg gtattttccg tagtggctgt tcgggttgtt
     3121 gtgcctctcg gtactgtggt atttgtgttt cggggctgcg tgtttatctc atcattgatc
     3181 ctgcccgaag tgcccgaggt cctcgaactt gttacgccgg ctgtacgttg gttgttaatt
     3241 cgattggtgg tgttaacctg catctggcgg tcgctgggtg agtatttgaa cctgcggttt
     3301 tcgcggatgt tgttgtcatt ccagtcccgt gcccgttcgc gctggttttc gggccagtaa
     3361 acggcgctct ttgcattttc gttgaatcca cgaacatacg gaatattgct gtaatcgtaa
     3421 taatcgcggt aataatacct cggataatgt gaaatatagt actggtgatg catccagggc
     3481 tgataaattc tggtgttgac tatgattacg aagccgtaca tcggatcaaa atcgttgtaa
     3541 aatcccaaaa gagtaggagt gaaaaaccac tgtccgcgat ttagaaatat atattcacgt
     3601 gttgccaggt cgtaataaca ttcgatatcc ggaaaataat aataacgggt tccagggtag
     3661 tattcaggtg cccattccgg attttcgtaa taggtttgtt cgttggtagt tccgtagttg
     3721 taggtgttac aaccggaaaa gagcgataca gcaactaaaa cagtaacagc aagaaccata
     3781 aatgagataa gttttttcat gattttaatg ttttataaag tttaagataa tttacttaag
     3841 ttaaaacaaa taccgtaccg aaaactgttc aacaagatgc ctgtttattt taatttgtaa
     3901 atatatacaa aaaaaattgc ctcagcattc accaggcaat tttttttaat aaaaatatat
     3961 tttttaattt tctcagaatg atacagttgc aactttatct gtcaccgtaa aagtttttac
     4021 aagcgttcca ttgctgtatg ttccatcttt cgaatatccg tttactgtag ttcctccatt
     4081 gtaagttcca cccttaaata cgttgtagtc accagttttt agatcgggag aggagagaag
     4141 taaaccataa gcacttcgaa tgggtttgaa agtcacgatc gatttgcctg aagcatcctg
     4201 tatattgatg agatttgtgg ccatagtgga ggatgtgagc ttaacggaat tctgacttga
     4261 ttctgttccg ggtgttttaa ccgatagaat tgactgaggt cctgaggcaa tcagaaatcc
     4321 acccgatatg gtaaattttc cattataatt gatgccttcc ttgtccttgt cggatggacc
     4381 ctgcaccact accgttccgc ctgacatggt gatgtcgcca ctgctgtcaa tgcctgttcc
     4441 atcatctgag ttgatgtaca aatttccgtt cgatatcgac aggatgcttc catcggttgt
     4501 tattccacca aagccgtagg tggcattcac tccggcttta gttgaaatta tcgatgttgt
     4561 gccacctgat atagtaataa atggactttc gatccctttt ttcgaagcgt tgatctgaac
     4621 tgttccgcct gatatatgta ccagtgagtc ggagcgaagc gcctggctgt tgagtgacct
     4681 ggcggtaata ttccctcctt caatgatcag tcttttggag gtatggatgc catcgccatc
     4741 ggatgatgtg aggcttaatg ttcctccagc aattttcaga tatccttttc ccggtttcag
     4801 tacgttgtcc gatttcagtg catgaccggt agagcatatc acattgatat tgccgttgtt
     4861 tacaatcagc gagtctttac cccgtattcc gtcatcaata gattttaccg aaaagttgcc
     4921 attgtcaatg taaagcatat cgtcgctcga aatagcgtca ttgtaatttc cgttcaccac
     4981 caggttgcca tcgcccgtaa atccgagata atctttgctg aaaattgctg cgttaggatc
     5041 accaacgtgt gtgtaggcag cgccgtctgt aaatgtgtta gtagttccgg ttttcagaaa
     5101 aataattgtt ttttttgcat cccgtatgaa taatggggag gtagtgtcgt tattcatggt
     5161 aattccattg aactgtattt ttaccacctg atctttggca tcaacaatca gttgtccgtt
     5221 agtcatttgt ccgcttacgg aatagtaacc gccaccggta attgtagctg tagagcccgt
     5281 aatgattacc tttgcagatg aagaactgat ggatgcagtg ctgaatgtaa tggtttgagt
     5341 tttagcatca tcccattcaa acagtagttt tgtgtctttc tctataggtt tcattacatt
     5401 ttcggggtca caggaactca ataaaaatgc cgtgaaagct aacaggaagt atgagaattt
     5461 tagtttcatg attatagaaa aataaatcag agttatgcgg gagtatttaa ttgcactatg
     5521 gttattttaa ctcgtgtgta aagctcagac cgaggttgta tttgcccgaa tagtcagctt
     5581 catcggtgcc tttttctgaa tttaaaactc caaatacttt gattttattg tgtttattaa
     5641 tcttatagtc cgtgtacaga atatatctta gtttgtccag ttt
//
LOCUS       DAOPZK010000124         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_17916_length_5644_cov_23.794239, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000124 DAOPZK010000000
VERSION     DAOPZK010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17916_length_5644_cov_23.794239"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1241)
                     /gene="xylA"
                     /locus_tag="PKH68_07420"
     CDS             complement(<1..>1241)
                     /gene="xylA"
                     /locus_tag="PKH68_07420"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNX12804.1"
                     /translation="KFEGKESKNPLAFRYYDAEKVVYGKPMKEWFKFSMAWWHTLCAD
                     GGDPFGGQTQKHPWVGAADALQAAKDKMDAGFEFMQKAGIEYYCFHDIDLISEGNSIE
                     EYEANLKAIVAYAKQKQAETGIKLMWGTANVFSHARYMNGASTNPDFDAAARAMVQIK
                     NAMDATIELGGKAYVFWGGREGYMSLLNTNMKREKQHMGMMLTKARDYARAKGFKGVF
                     LIEPKPMEPMKHQYDVDAETVIGFLKEFGLEKDFKLNIEVNHATLAGHTFEHELQCAV
                     DAGMLGAIDANRGDVQNGWDTDQFPVDVYDLTQAMLVVLQGGGMQGGGTNFDAKIRRN
                     STDNEDLFIAHVGAMDAMARALEAAAEILEKSPYKKMVSDRYASYDAGKGKEFEDGKL
                     SFEDVYAYAKSKGEPKQISGK"
     assembly_gap    1242..1341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1342..2703)
                     /locus_tag="PKH68_07425"
     CDS             complement(<1342..2703)
                     /locus_tag="PKH68_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNX12805.1"
                     /translation="MYLLGYDLGSSSVKASLVNAATGECISTAFFPKKEMEIISLKSG
                     WAEQNPDTWWENLKASTQAVLAESKISPADIGAIGISYQMHGLVLVDENLRAIRNSII
                     WCDSRAVPYGEKAFNALGADKCLSHLLNSPGNFTLSKLAWVKENEPENYTKIKYYMLP
                     GDYIATRMTGKACTTASGLSEGIGWDFKAETPAGFLFDYFGFNQSLIPDIVPTFGQQG
                     TLKADIASELGLAEGTPITYRAGDQPNNALSLNVFNPGEIAATAGTSGVVYGVNSDSK
                     YDPQSRVNSFAHVNHTKKDTRIGVLLCINGTAILNTWIKNNVAPEGIGYAGMNELAAA
                     IPVGSEGVSIVPFGNGAERVLGNKEPNCSIHGVNFNRIGKAHLIRAAHEGIAFSFKYG
                     MDIMNEMGIETKTIRAGYANLFLSPIFRQTLANITGATIELYNTDGSIGAARGAGIGA
                     GIYK"
     gene            complement(2841..3542)
                     /locus_tag="PKH68_07430"
     CDS             complement(2841..3542)
                     /locus_tag="PKH68_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNX12806.1"
                     /translation="MTTENLYNPHVSVDCVVFGFDGENLKVLLIERNIQEQNENYNDK
                     KLPGSIIFNDEDLDDAASRVLTELTGLKNIYLSQFHSFGNPNRINNPRDILWLENTMK
                     LKIGRIVTVGYMALIKIDRKIQVLPDNTIANWYDVKQLKNMSIAFDHAEIIEKALEHI
                     RFNMDRDPQLFFELLPRKFTMSQLRQLFDKIHNTRSDIRNFQKKVAQWPYVVPLNETE
                     TGVPHRAARLYKFKK"
     gene            complement(3776..5023)
                     /locus_tag="PKH68_07435"
     CDS             complement(3776..5023)
                     /locus_tag="PKH68_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12807.1"
                     /translation="MKKTLLFISLFCMSLYASAQAPVKITFESDAVGSAGGATAVWSA
                     GSVDVVANTYTTGNPSAKALHVLNTGYLGVYFGNIPLPAGAETMYSKIKVKYLIVGGT
                     DKDYPLLEIFSAPNNYTMGDPEKFGEVPWAGLWGTAEIGVWKTVEFAFANVTKAVPAG
                     NLILKLSKSNTEYLIDDVELVPSVAPAPVITLADFEANTIGEVLTMRRWTPTDASATV
                     EANPTVASEKSVHILATNWNSALKLNIALPTGKTIADYETLSFDIYLNNIDGTDNVWK
                     NIEVYVDDVKKIDKQSPGVANAWEAKSYSLEGITSANSLVMDIGLNTNKGNYYLDNIK
                     ITEKASTGISPSAVNPLFVYSASNTFVLNQQVDTYELYNVQGSRISAGRNASSINTTN
                     LGNGIYILKASIGNDKFVTKLLK"
     gene            complement(5355..>5644)
                     /locus_tag="PKH68_07440"
     CDS             complement(5355..>5644)
                     /locus_tag="PKH68_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNX12808.1"
                     /translation="QWLAMEMAMKYGDKVRVNALAPGFFIGDQNRAVLINPDGSLTDR
                     SQKVLANTPMRRFGDINELNGAVQFLCSDAASFITGVVLPVDGGFSMFSGV"
BASE COUNT         1467 a         1238 c         1107 g         1732 t
ORIGIN      
        1 tgtttgccgc ttatctgttt gggttcacct ttagatttgg cataagcata cacatcttcg
       61 aatgaaagtt ttccgtcttc aaattctttg ccttttccgg catcataaga tgcgtagcga
      121 tcagaaacca tttttttgta aggagatttt tcaagaattt cagcagcagc ttccaatgca
      181 cgtgccatag catccatagc acctacgtga gcaatgaaca gatcttcgtt atcggtagag
      241 ttacgacgaa ttttagcatc gaagttggta ccgccaccct gcatgccacc gccctgcaat
      301 actaccagca tagcctgagt taaatcatat acatctaccg ggaactggtc ggtatcccat
      361 ccgttctgaa catcaccgcg gttggcatcg atggcaccca gcataccggc atccactgca
      421 cattgcagtt cgtgttcgaa agtgtgtccg gccagtgtgg catggttcac ttcaatattc
      481 aatttaaagt ccttttccaa tccaaattcc ttcaggaaac ctattactgt ttcggcatcc
      541 acatcgtatt gatgtttcat tggttccatg ggttttggtt caatgaggaa tacacctttg
      601 aaacctttgg cacgtgcata gtcgcgggct ttggtcagca tcattcccat gtgttgtttt
      661 tcacgtttca tattggtgtt caacagcgac atgtaacctt cgcggcctcc ccagaataca
      721 tatgctttac cacccaattc gatagttgca tccatggcgt tcttgatttg aaccatggcg
      781 cgtgcagctg catcaaaatc gggattggtg ctggcaccgt tcatgtaacg ggcatgtgag
      841 aaaacattgg cagttcccca catcagttta atgccggttt cagcctgttt ttgtttggca
      901 taagctacaa ttgctttcaa attagcttcg tactcttcga tgctgtttcc ttcactgatc
      961 aagtcaatat cgtggaaaca atagtattcg atacccgctt tttgcataaa ttcgaatcct
     1021 gcatccattt tatctttagc tgcctgcaaa gcatccgctg caccaaccca ggggtgtttt
     1081 tgtgtttgtc cgccaaaggg atcgccacca tctgcacata acgtatgcca ccaggccata
     1141 ctgaatttga accattcttt catgggttta ccataaacca ctttttcggc atcgtaatag
     1201 cggaatgcca acgggttttt actttcttta ccttcgaatt tnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn ntttatagat tccggcaccg atccctgcac cacgggctgc
     1381 acctatcgac ccgtcggtat tgtagagttc aatggtggca ccggtgatat tggccagtgt
     1441 ctgacggaaa atggggctca ggaacagatt ggcatatccg gcacgaatag ttttggtttc
     1501 aatgcccatc tcattcataa tatccatccc gtatttgaac gaaaatgcaa tgccttcgtg
     1561 agcagcccga atgagatgcg ctttaccgat gcggttgaaa tttacgccgt gaatagagca
     1621 gtttggttcc ttattgccca gtacgcgttc ggcgccattg ccaaaaggaa caatactcac
     1681 cccttcgctt cctacgggaa tagcagctgc cagttcattc attccggcat atccgatgcc
     1741 ttccggcgct acattatttt taatccaggt attgagaatg gctgtgccgt tgatacaaag
     1801 cagcacacca attcgggtat ctttttttgt atgattaaca tgtgcaaatg agtttaccct
     1861 tgactgtgga tcgtatttag agtcactgtt gacgccatag accacgcccg aagttcctgc
     1921 tgtagcggca atttcaccgg gattaaatac gtttaatgat aatgcgttat tgggctgatc
     1981 gcctgcacgg tatgttatgg gagttccttc ggccaatcca agttcagaag caatatcagc
     2041 tttcagcgtg ccttgttgtc cgaatgttgg aacaatatcg ggaattagag actgattgaa
     2101 accgaaataa tcaaacagaa aaccggcagg agtttcggct ttgaaatccc agccaatccc
     2161 ttcggataat cctgaagcag tggtacaggc ttttccggtc atacgtgtgg caatataatc
     2221 acccggaagc atataatact tgattttagt gtaattttcc ggttcgttct cttttaccca
     2281 ggctaatttc gacaaggtaa agtttccggg cgaattgagc agatgtgaca aacacttatc
     2341 agcaccaagg gcattgaatg ctttctcacc gtatggtaca gccctgctgt cgcaccagat
     2401 gatggaatta cggattgccc tcagattttc atccaccagc accaacccgt gcatctgata
     2461 agaaatcccg atagccccga tatcggcagg tgagatttta ctctcagcca ataccgcctg
     2521 agtggatgct ttcagatttt cccaccatgt gtcgggattt tgttccgccc atccgctttt
     2581 cagcgaaatt atttccattt ccttttttgg gaaaaatgca gtggatatac attcgccggt
     2641 ggcagcatta accaaactcg ctttaacaga cgaactacct aaatcataac ctaatagata
     2701 catatatttt tatttgaagc cccctaaatc ccccaaaggg ggacttaaag accatgttta
     2761 ttatatttct aacattttat attttacgaa taaccctttt aattattctc ttaaaattca
     2821 ctcttgggta attgaaggaa ttactttttg aatttgtaca agcgggctgc gcggtgaggc
     2881 acacctgttt cagtttcatt gagcgggact acatacggcc attgagccac ctttttctga
     2941 aaattacgga tatcggaacg ggtgttgtga attttgtcaa acaactgccg gagttgcgac
     3001 atggtaaact tgcggggcag cagttcaaaa aacagttgtg gatcacgatc catattaaaa
     3061 cgtatgtgct ccagcgcctt ttcaattatt tcggcatggt caaaggcgat ggacatattt
     3121 ttcaactgct tcacatcata ccagttagct attgtattat cgggcagtac ctgaatttta
     3181 cggtcaattt ttatcagtgc catatacccc acggtcacaa tgcgtccgat tttgagtttc
     3241 atggtatttt cgagccacag aatatcccgc ggattattga tacgattggg attaccaaaa
     3301 ctgtgaaact ggctgaggta gatatttttc agcccggtga gttcggttag tacccgcgat
     3361 gctgcatcgt caagatcttc gtcgttgaaa ataatactgc cgggaagttt cttgtcgttg
     3421 taattttcat tttgctcctg aatgttgcgt tcaatcaaca agactttcag gttttcaccg
     3481 tcaaatccga aaacaacaca gtcgacagaa acatgcggat tatacagatt ctctgttgtc
     3541 ataatttctt gttttatccg atgcaaaaat aattctttta tttatttatt attcattttt
     3601 cagtatgtta tacaacacat ttttagcact ttgaatatgg tattttatac aatatcacga
     3661 tttaggttat tgtttttaat acaatatgta tttttttcga tttttctatc aatttgcagc
     3721 aaaaaaaggt gtcacaccgg acaccttttt tacactaaaa aaaacttgga aaaaattact
     3781 ttaacaattt agtgacaaat ttatcgttac caattgaggc tttcagtata taaatgccat
     3841 tacccagatt agtggtatta atgcttgagg catttctgcc ggcagaaatt ctggaaccct
     3901 gaacgttgta aagttcgtat gtatcaactt gctgattaag cacaaacgta ttggaagcgg
     3961 aatacacaaa aagtggattt actgcagaag gtgagatccc tgtagatgct ttctctgtta
     4021 tttttatatt atccagatag taattacctt tatttgtatt caggccaata tccattacca
     4081 gactatttgc agatgttatt ccttccaggc tgtaagattt tgcttcccag gcattggcga
     4141 cacccggact ttgtttgtca atttttttca catcatccac ataaacttct atgtttttcc
     4201 agacgttatc ggtaccatca atattattca ggtaaatatc gaacgataat gtctcatagt
     4261 cggctatcgt ttttccggtt ggtagagcaa tgttcagttt aagagccgaa ttccagttgg
     4321 ttgccagaat atgaaccgac ttttcggatg caacagtcgg attagcctca accgttgctg
     4381 atgcatcggt tggagtccat cttctcatag ttagcacttc accaattgtg ttcgcttcaa
     4441 aatcggctaa ggtaataacc ggtgcaggag caactgaagg aacaagttcc acatcatcaa
     4501 tcaggtattc ggtattactt ttcgacaatt ttaagattaa attacctgcc ggcacggctt
     4561 tagtgacatt tgcaaaagca aactcgacag ttttccaaac cccgatttca gcagttcccc
     4621 acaatccggc ccatggaact tcgccgaatt tttcgggatc acccatcgta taattattcg
     4681 gggctgagaa tatctccaat aatggataat ctttatccgt tccaccaact atcaggtatt
     4741 ttaccttaat cttcgaatac atcgtttctg caccggcagg caatggtata tttccaaaat
     4801 atacacccag atagccggta ttaagaacat gtaatgcctt tgcagacgga ttacctgttg
     4861 tataggtgtt tgcaaccaca tctacacttc ctgcactcca cacggcggta gctccgccgg
     4921 cagatccaac agcatctgat tcaaaggtaa ttttcacagg tgcctgagca cttgcataca
     4981 gactcataca aaataaagaa ataaagagta aggttttttt catgattcat tgaatttaat
     5041 aaattaatac ttggttaatt gcgttttatc ttttttaggg tttttgattt tacaaatata
     5101 acctctggct atcaaattat atatcctatt tgttacatgt tcttgttcgg atgtttccta
     5161 aagagggtgg caaagttaag aaacctgtgg tttttccaat atcccttcat ttcttcaacc
     5221 tttaaccgaa tatggaattt atctcttact ccggcatacc tatagaaaaa ggaactaccg
     5281 gcatccatta accggttgtt ccttcatgta ttattgttgt ggttaagttt gttttctgtg
     5341 aaaatacatc tgttttacac accgctgaac atgctgaagc ctccatccac aggcaaaact
     5401 acgccggtga tgaatgaagc tgcatcggaa caaagaaatt gtacggctcc gttcagttcg
     5461 ttgatgtcgc caaagcgacg catgggagta ttggcaagca ctttctgact gcggtcggtg
     5521 agagagccgt cggggttgat aagtacggcg cgattctggt cgccaatgaa aaaacccggt
     5581 gccagtgcgt tgacacgtac tttatcgccg tacttcatgg ccatttccat agccagccat
     5641 tggg
//
LOCUS       DAOPZK010000125         5555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_18345_length_5555_cov_20.612727, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000125 DAOPZK010000000
VERSION     DAOPZK010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18345_length_5555_cov_20.612727"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKH68_07445"
     CDS             <1..566
                     /locus_tag="PKH68_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12809.1"
                     /translation="ACVGVGGILPLPKRFSLSLEILYDEKGAYQKLPASPAGNNQLPY
                     YSLKWNYLTAPLMAHYEDRNTWTFGLGVAWGRMVTFKEKEWNITKPWDRPDTITYKTK
                     NDFSVIADLRFRIWKHLKFNIRYSYSIVPVRKRDYVNPTTFETWSRKQYNNVITIRLL
                     YYFNEKYLPDRFTFKKKSKKKNSQIEK"
     gene            578..1084
                     /locus_tag="PKH68_07450"
     CDS             578..1084
                     /locus_tag="PKH68_07450"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX12810.1"
                     /translation="MSYTADFWIAQLQLQPHPEGGYFKEVYRSEEMINAAFLSNRYQS
                     ERCFATSIYFLLKSGQISAFHRLQSDEIWHFYSGSSIRVYIINAAGILSETILGRDVL
                     SNELLHLVIPHGCWFAAEVNRADSYSLIGCTVAPGFDFRDFELATKAGLTELFPSYSR
                     LFEKMCIR"
     gene            complement(1120..1389)
                     /locus_tag="PKH68_07455"
     CDS             complement(1120..1389)
                     /locus_tag="PKH68_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNX12811.1"
                     /translation="MSTLTDEIKQHIIEELNLTHELDEISHETPLFSNRYTHLDSLDA
                     LRIIVMLEKKYGIKIKDLSQRKKILYSLDAIAKLVEEDRQQQARQ"
     gene            complement(1405..2694)
                     /locus_tag="PKH68_07460"
     CDS             complement(1405..2694)
                     /locus_tag="PKH68_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX12812.1"
                     /translation="MKTFLASIKKEFLVLLRDRSGLGILFIMPLALVVIMALVQDGPY
                     KNFQKSEYPMIFVDKDQDTVGAVIERNIRDAHIFKINTEINGIPATEKTAREAVAQGK
                     FRAGVVVPAGLSKYLRKNARMLVAKTFSGLPAEKASLAPEAPDSMNVKYFLDPLTTFS
                     FKNTIVYILEKSLSSLISQTLTTNYYKHFENFYPIENKINFDSCRIIGMQEISASSTY
                     NEHVVFNSVQHNVPAWTMFAMFFIFLPLAGSIIKERDYGTMIRLRFMPGSFSTLIAGK
                     IFLYTLVGCIQFLMMLTAGRCLLPAIGLPALIIGHSYAAIAATVLAASLAATGFGVLI
                     GTFFSTHQQMVTFGSISVIILAALGGIFVPIYIMSKTMAKVSKYSPLEWGLNAFNEIF
                     LHQGTFINIYIDALKLLLFSMAAFLLSFLYYKFKRIH"
     gene            complement(2696..3445)
                     /locus_tag="PKH68_07465"
     CDS             complement(2696..3445)
                     /locus_tag="PKH68_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX12813.1"
                     /translation="MDKETILQVEKLSKYYKGNVYPAVDNVSLAIRRNEIFGLLGPNG
                     AGKTTLISVMCGLFKPDSGNVLFNGSSLHQHLKSLKKIIGMVPQDIALYPTLTARENL
                     LFFGHVYGLPRKFLDNKINQCLAILELEKSKNKKINTFSGGMKRRINIAAGLLHEPQL
                     LFLDEPTVGIDVHSKKVIINYLKDINKNGTTILYTSHLIDEAEDFCSYIAIMDQGKIV
                     TSGKPADLISSNKNTKDLEDVFLQLTEKSKP"
     gene            complement(3455..>4267)
                     /locus_tag="PKH68_07470"
     CDS             complement(3455..>4267)
                     /locus_tag="PKH68_07470"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HNX12814.1"
                     /translation="IMPGWLSRIFKNRFHLEIIKKQYRTQGAAMDDLDNLLEQNIPVG
                     LVTNLFFIPYFPDSYRFHFNNHHMIVYGKENGHYLVSDPIFDSPTTISHEDLCTARFA
                     AGLRSLKGDLFYIGNIPQDYDLKKIIHRAIRYTSKNLLTPMPLNGYRGIRYLSKRVNQ
                     WPRKMDEKKAKRYMGNIIRMLEEVGTGGSGFRYMYAAFLQEAANITDKDLLLEASAGM
                     TLAGDTWREFAYQAATICKTKQPGEHMYHEASAILLRCSETEESVFRKLSLL"
     assembly_gap    4268..4367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4368..5242)
                     /locus_tag="PKH68_07475"
     CDS             complement(<4368..5242)
                     /locus_tag="PKH68_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12815.1"
                     /translation="MNQRRRNKIDEIVNYLKRKAVQDPASASAIAENITFFNNTYSLH
                     SLYKWLFTNAPSIKAENLKELTDIDSASPYFSELITTLAATEKKFFPGLVRPMVKKIT
                     QLIRDKNYSVIISIGAGAMEVEKQVIKKLIRLGNQKPVIFIGIDKSDISRKLAKENLS
                     ASPDTLIIERNLLDEVRLEEINKGTSSLYTVVLCNNDIFSLDKYFTGTSFDLAYNCFF
                     KHHFTPEEARHIDQMLSRHSKEVIDYDGVKNRFNTFVQPLFVWNNPVLLSGTVFSNLR
                     YKTIKELKVQNNDEK"
     gene            complement(5257..>5555)
                     /locus_tag="PKH68_07480"
     CDS             complement(5257..>5555)
                     /locus_tag="PKH68_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12816.1"
                     /translation="PSLGWEPEHFAQKQAWDSVAEDIEQLKEKVIAGQLSPIAYFIEK
                     YHFSLRRLSHLTGFSKRQIKKHFKPEVFSALNSTDLEIYARAFQMPVDQIVKPF"
BASE COUNT         1378 a         1142 c         1254 g         1681 t
ORIGIN      
        1 gagcctgtgt tggtgtggga ggaattttgc cgctacccaa acgattttca ttaagtctcg
       61 aaattttgta cgatgaaaaa ggggcatatc agaaactgcc tgcctccccg gcaggaaaca
      121 atcagctgcc ttactactcc ttaaaatgga attacctcac ggcaccgctc atggcgcatt
      181 acgaagaccg caacacctgg acctttggcc tgggtgtggc ctggggacgt atggtaactt
      241 tcaaggaaaa agaatggaat attacaaaac cctgggatag gcccgatacc attacctata
      301 aaacaaaaaa cgattttagc gtcatcgccg acctgcgttt tcgtatctgg aagcatctga
      361 aatttaacat acgttattct tattccatcg ttcccgtacg taaacgtgat tatgtgaatc
      421 ctaccacctt cgaaacctgg tcgcgcaaac agtacaataa cgtaattacc atccgcttgc
      481 tttactattt caacgaaaaa tacctgcccg acaggtttac tttcaagaaa aaaagcaaaa
      541 agaaaaattc tcaaattgaa aaataattta cctgctgatg agctatacgg ctgatttctg
      601 gatagcgcaa cttcaattgc agcctcaccc cgaaggcggc tactttaaag aagtctatcg
      661 ttcggaagaa atgatcaatg cagcctttct ttctaacaga tatcaatccg aacggtgctt
      721 tgcaacatcc atctatttcc tgctgaaaag cgggcagata tccgctttcc acaggttgca
      781 atccgacgaa atatggcatt tttattcagg ctcctccata cgggtttata tcataaacgc
      841 tgccggaatt ttgtcggaaa ccatactggg ccgcgacgtt cttagtaatg agttattaca
      901 tttggtaata ccccatggat gctggtttgc agccgaagtt aaccgtgctg attcttattc
      961 tttgatagga tgtaccgtgg ctcctggatt tgatttccgg gattttgaac tggcaacaaa
     1021 ggcagggctt acagagcttt ttccttcata cagccgttta ttcgaaaaga tgtgtatccg
     1081 gtaatttttt tgtgagtgtg ggcgctgtct tttgcaggct tactgccggg cttgttgttg
     1141 gcggtcttcc tccactaact ttgcaatagc atcaagagaa taaagaattt tttttcgttg
     1201 cgacaggtct ttaatcttaa tgccatattt tttttcgagc ataacaataa ttcgcaatgc
     1261 atcaagcgaa tccagatggg tgtatcggtt gctgaacaag ggtgtttcgt ggctgatttc
     1321 gtccagctcg tgcgtcaagt tcagctcttc gatgatatgc tgttttattt cgtcagtgag
     1381 ggtgctcatg ataagaagcc tgatttaatg tattcttttg aatttataat acaaaaatga
     1441 tagcaaaaat gccgccattg aaaacagtag caattttagg gcatcaatat aaatatttat
     1501 gaaggtgccc tgatgcagaa aaatttcgtt gaaggcattt aacccccatt ccaatggcga
     1561 gtatttactc acctttgcca tagttttcga catgatgtaa atgggaacaa atattccccc
     1621 cagagcggcc agaattatta ccgagatgga accaaaggta accatctgct gatgagtcga
     1681 aaaaaaagtt cctatcagta cgccaaaacc tgtagctgcc agtgaggccg ccagcactgt
     1741 ggccgcaatg gctgcataac tgtggcctat tatcagggcg ggcagcccta tggccggcag
     1801 caggcacctt ccggcagtca gcatcataag aaactgaata cagccgacca gggtatataa
     1861 aaatattttt ccggcaatga gggtggaaaa acttcctggc ataaaacgca gcctgatcat
     1921 tgttccgtag tcgcgctctt tgatgatgct tcccgccaga ggcagaaaaa taaaaaacat
     1981 ggcgaacata gtccatgcgg gtacattgtg ctgcaccgag ttgaaaacaa catgctcgtt
     2041 atatgtgctg gaggcgctga tttcctgcat gccgataatc cggcaactgt cgaaatttat
     2101 tttgttttca attgggtaaa agttttcaaa atgcttgtag taatttgtcg taagagtttg
     2161 tgaaatcagc gatgacagcg atttttcgag gatgtaaacg atggtgtttt tgaaggagaa
     2221 tgtggttagc gggtccagaa aatattttac attcatcgaa tccggcgctt caggagccag
     2281 gctggctttt tctgcgggca gtcccgaaaa agtttttgct accaacatac gggcattttt
     2341 tcgcaggtat tttgaaagtc cggcaggcac taccactccc gcccggaact ttccttgcgc
     2401 aacggcctca cgggccgttt tttcggtggc tggtattccg ttgatttcgg tgtttatctt
     2461 gaaaatgtgg gcatccctga tgttcctttc gattacggca ccaacggtat cctggtcctt
     2521 gtccacaaaa atcatcgggt attcggattt ctgaaaattc ttgtatggcc cgtcctgcac
     2581 caaagccatg atgacgacca gcgcaagcgg catgataaaa agtatcccca gccctgaacg
     2641 gtctctcagc agcaccagaa attctttttt tatcgaggca aggaatgttt tcatactagg
     2701 gtttggattt ttcagttagt tgcagaaaaa catcttccag gtctttggtg tttttattgc
     2761 tacttatcaa atcagctggt tttccgctgg tcacaatttt tccctggtcc atgatggcga
     2821 tataactgca aaaatcttcg gcttcatcaa taaggtgcga tgtgtacaga atggtagtgc
     2881 cgtttttgtt gatgtctttc aggtagttga tgattacctt tttggaatgt acatcaatgc
     2941 caacggtagg ttcgtccaga aacagcagtt gaggttcgtg cagcaggccg gccgcgatgt
     3001 tgattcggcg tttcatgcct cccgaaaacg tgttgatttt tttgtttttt gatttttcaa
     3061 gttccagtat ggcaaggcat tgattgattt tgttatcaag aaacttcctt ggcaaaccgt
     3121 acacatggcc gaaaaaaagc aggttttccc gggctgtcag ggtggggtac agcgcgatgt
     3181 cctgcggtac catacctata atttttttta gtgatttgag gtgctgatgc aagctgctgc
     3241 cgttgaacaa aacgttcccg ctgtcgggtt tgaacaggcc gcacatcacc gatatcaggg
     3301 tggttttacc tgctccgttg gggccaagca atccaaaaat ctcattacgc cttatcgcaa
     3361 gagaaacatt gtcaacggca ggataaacgt tgcctttgta gtattttgat agtttttcaa
     3421 cttgtaatat tgtttccttg tccatcgtaa gtgcctaaag tagtgatagt tttctgaaga
     3481 cggattcttc ggtttccgaa cagcgtagca atatagcgga ggcttcgtga tacatatgct
     3541 ctccgggctg cttggttttg cagatggtgg ctgcctggta ggcaaattcg cgccaggtgt
     3601 ctccggccag cgtcattcct gccgaagctt ccagcaacag gtctttgtcg gtgatgttag
     3661 cggcttcctg cagaaaagct gcgtacatat agcgaaatcc cgaaccgcct gttcctactt
     3721 cttcgagcat gcggataatg ttgcccatat agcgcttggc ttttttttca tccattttcc
     3781 gtggccattg gtttacgcgt ttcgacaggt atcgtatgcc cctgtagccg tttaaaggca
     3841 ttggggttag taagtttttg ctggtgtaac ggatggctcg gtgaatgatt ttttttaaat
     3901 cataatcctg cgggatgttg ccgatgtaga aaaggtcgcc cttgagcgat cttaatccgg
     3961 cggcaaatcg tgcggtgcaa aggtcctcgt gcgatattgt ggtggggcta tcaaaaattg
     4021 gatcgctgac aaggtagtgc ccgttttctt ttccataaac tatcatgtgg tgattgttga
     4081 aatgaaaacg gtacgaatcg gggaaatagg gtataaaaaa aagattggta accaagccca
     4141 ctggaatgtt ctgttcaagc aggttgtcca ggtcgtccat ggcggcacct tgtgtacggt
     4201 attgtttttt gattatctca aggtgaaacc tgtttttgaa tattcgcgac agccaccctg
     4261 gcattatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt ttttcgtcgt
     4381 tgttttgtac ttttaattcc tttattgtct tgtaccttaa attcgaaaat accgttccgc
     4441 tgagcaatac agggttattc catacaaaca gcggctgaac aaaagtatta aacctgtttt
     4501 tcaccccgtc ataatcaatt acttcttttg aatgccgact taacatttgg tcaatatgcc
     4561 ttgcttcttc cggcgtgaaa tgatgcttga agaaacagtt gtatgccagg tcaaagcttg
     4621 ttcccgtaaa atatttatcg agcgaaaaaa tatcgttatt gcataacacc actgtgtaaa
     4681 ggctgctggt tcctttgttt atttcttcca acctgacctc atcgagcagg tttctttcga
     4741 tgattagggt atcggggctt gcgctaaggt tttcttttgc cagcttgcgg cttatgtctg
     4801 atttgtcgat gccaatgaaa attacaggtt tctgatttcc caggcggata agttttttga
     4861 ttacctgttt ttcaacttcc atggctcctg ccccgatgct gataatcaca ctatagtttt
     4921 tatccctgat taattgtgta atttttttga ccattggcct gaccaatccg gggaagaact
     4981 ttttttcggt agcggctaag gtggttatca gctcggagaa ataagggctt gcagagtcga
     5041 tgtcagttag ttctttgagg ttttcggctt tgatggaggg ggcattggta aacagccatt
     5101 tataaaggga atgtagggaa tacgtgttat taaaaaatgt gatattttca gcaatggcac
     5161 tggccgaagc agggtcttgc acggcttttc ttttcagata gtttactatc tcgtcaattt
     5221 tatttcgtcg gcgttggttc attcgtgatt agcgaactaa aatggtttta caatttggtc
     5281 aacaggcatt tgaaatgccc tggcataaat ttcgaggtca gtggaattga gtgctgaaaa
     5341 aacttcgggc ttgaagtgtt tttttatctg acgtttagag aatcctgtca ggtgcgacaa
     5401 ccttcgcagc gagaagtggt atttttcaat aaaatatgct attgggctga gctggccagc
     5461 tattactttt tcttttaatt gttctatatc ttctgccacc gagtcccagg cttgtttttg
     5521 ggcaaaatgt tcaggttccc agcccaggct tggca
//
LOCUS       DAOPZK010000126         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_18531_length_5516_cov_24.898553, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000126 DAOPZK010000000
VERSION     DAOPZK010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18531_length_5516_cov_24.898553"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>4224
                     /locus_tag="PKH68_07485"
     CDS             128..>4224
                     /locus_tag="PKH68_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNX12817.1"
                     /translation="MLEKIIGFSLKFKLIIILFTLTIAAFGIYAILNIPVGAVPDITN
                     NQVQVITTSGNLSTQEIEQFITAPVELEMANLPGVKEIRSVSKFGLSVVTIVFSENMG
                     TYLPRQLIAEKIKSASANIPEGFGTPEMGPITTGLGEIYQYVLDVKPGYEDRYTAMDL
                     RTIQDWIVRRQLSGINGVVEVNSWGGYLKQYEVAVNPARLQALNISLMEVFDALESNN
                     SISGGAYIEKNAESYFIRGDGQVKSLEDIEKIVVKNQGGVPVLVRDVATVHFGYANRF
                     GAITANGEGEKVLGQIMMLKNANSKMVISEVKSRVAEVQKSLPEGIYINPILERSELI
                     GKTTLTVFENLIFGCIIVFLVVLLLLGNLRSALVIASMIPLALFFTLSMMFIFGIDAN
                     LMSLGALDFGIIIDGAVIIVEFIAVRMLANKEKLNALKGEEHQSLMDKITLNGASKMM
                     TSAIFGQVIILIVFIPILSLQGVEGKMFRPMALAFSFAIIGAMFLCLTWLPVAASMFL
                     KPQEPGKKNISERVFRFLLSTYKPVMNWSCDHKALVLGVSFSTLILAFIIFGRMGGEF
                     VPTLDEGDFVIQPVLKTGTSLSKTVELTTQMENILIDKFPEVEKIVCRIGAAEVPTDP
                     MSMEEIDMIIKLKPKKEWKSADTKEGLADKFKEALSVIPGIDYEFTQPIEMRFNELIT
                     GVRSDIAVKVFGEDLDYLNEKASEIKQLIEGIPGAADVILEKTTGLPQMNVKYNREKV
                     AFYGVDMKTMNAYLAAAFGGEIAGSIFEGEKRFDLVVRLQKENRTDIENIRQLQVPLP
                     NGMQVPLSELAEIRLTEGPAKISRENTHRRVVVSVNVRNRDLQSVVKDIQASVDKNVK
                     LNPGTYIQYGGQFENLQNATKRLIFAVPIALALIFIFLHFAFKSLKDTIMIFTAVPLS
                     VVGGVFALWLRGMPFSISAGVGFIALFGIAVLNGIVLIEHLKHLKETGMTNMRELVMK
                     GTVDRLRPVMLTAASAAMGFLPMAISTSSGAEVQRPLATVVIGGLITSTLLTMIALPL
                     LFMIFNDEEGLKYKFVRKFKLKNFLIPLALMLSGGALMAQSSSLTLEQAIDLALKNNR
                     EIGAYNLKVEERKALAKSLLEMDKTAVYYGYDQANVAENGHPIRTVGVEQNFLFPTIH
                     APRNKARKIEISMAETELQKQMLLLVKNVTGTYYTIQFLQAKIKHYQKMDSLYSVLNT
                     GAETRYKLGDISQLDLMNAQAKKQQVSLELNQINYDIQNTYRTLNALMQTSETYTVAS
                     DEMSVIPVVEPDFQQNADVRLLRLQSDYIKTNVKTEQQRLLPDISLGYFYGNNRFENN
                     RGYHGFQVGLGIPLYAGEQNAKIKAGKIAANTTELLLQNDLLLLET"
     assembly_gap    4225..4324
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4476..>5516
                     /locus_tag="PKH68_07490"
     CDS             4476..>5516
                     /locus_tag="PKH68_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNX12818.1"
                     /translation="MKDLKRISLIALALVLLAACGGKKATDKTENAPKEELIELTQEQ
                     FSTSGMELGEPSIQSFRDEVACKGYIIAPPNSMAKVSPPIPGVVNAIRCKTGDFVRAG
                     QVVCTISGNEFLSLQQDFAETAARFSKSKLDYERAKALREEKIGSEKDFIAVQSEYKT
                     VLASYNALKVKINAIRLDAARIENGQMYTSFPVVSPISGYVTKTSAVIGQYADMQNEL
                     VEIVNVNGLQLQLAVFETDIQKLRAGQAVHFGISGSTSKSLGATLITVGKTINPDTKV
                     IDCIARIDRASDAQLVNQSFVEATVIVNNKSAKALPVSAVQKVDKAYFIYVLERKEGE
                     KFLLKKTPVEVGA"
BASE COUNT         1524 a         1169 c         1324 g         1399 t
ORIGIN      
        1 ttatctaaaa caattctaaa tattttattt tgataccact gaatacattt agctgaataa
       61 tttttttaac tttgcgagta atagaattag ggtaaagaat aattttcgta cgttaaaatt
      121 caagtctatg ctcgaaaaaa taatcggttt cagtctgaaa ttcaagctga taatcatcct
      181 tttcacactt accattgccg ctttcggcat ttatgccatt ctcaacattc cggttggagc
      241 cgttcccgat attaccaaca atcaggtaca ggtcatcacc acttcgggta atctttccac
      301 gcaggaaatc gaacagttta tcaccgcgcc cgtggaactg gaaatggcca atctcccggg
      361 cgtgaaagaa atccgctctg tttccaaatt cggcttgtcg gtggtaacca tcgttttcag
      421 tgaaaatatg ggaacgtacc tgccgcgtca gctcatagcc gaaaaaatca aatcggcctc
      481 agccaatatc cccgaagggt tcggaacacc cgaaatgggc cctattacca ccggtttggg
      541 cgaaatatat cagtatgtac tggatgtaaa acccggttac gaggatcggt atacagctat
      601 ggatctgcgg acaattcagg actggatagt gcgacggcag ttgtccggaa taaacggggt
      661 ggtggaggtc aacagctggg gcggttacct caaacagtat gaagtggctg taaatcctgc
      721 acgcctgcaa gcgctgaata tcagcctgat ggaagtattt gatgcgttgg aaagcaacaa
      781 cagcatttcg ggcggtgctt acatcgaaaa aaatgctgaa agttacttta tccgtggcga
      841 cgggcaggtg aaatcactgg aggatattga aaaaattgtg gtcaaaaatc agggcggagt
      901 accagtcctt gtgcgggacg tggcaacggt gcatttcggt tatgccaacc ggtttggtgc
      961 cattacagcc aatggcgaag gcgagaaggt actcgggcaa atcatgatgc tcaagaatgc
     1021 caactccaaa atggtaatca gcgaagtgaa aagcagggta gccgaagtac agaaaagtct
     1081 tcccgaagga atttatatca atcctattct ggagcggagc gagctgatag gcaaaactac
     1141 gttaaccgtt tttgaaaatc tgatttttgg ctgcatcatc gtgtttctgg tcgtattgtt
     1201 gctgcttggc aatttgcgct cggcgctggt aatagcctcc atgataccgc tggcgttgtt
     1261 tttcaccctt tccatgatgt ttattttcgg catcgatgcc aacctgatga gcctgggagc
     1321 gcttgacttc ggaataatca tcgacggtgc ggttattata gttgaattta tcgccgtgag
     1381 gatgctcgca aataaagaga aactgaatgc attgaagggt gaggaacatc agtcgctgat
     1441 ggacaaaatc acgctgaacg gcgcatcaaa aatgatgact tctgccattt tcggtcaggt
     1501 gattattctt attgtattta ttcccatact ttcgttgcag ggcgtggagg gcaaaatgtt
     1561 ccgtccgatg gcactggctt ttagttttgc cattatcgga gccatgtttt tgtgcctcac
     1621 gtggctgcct gtggctgctt ccatgttcct caaaccgcag gagccgggca aaaaaaatat
     1681 ttcagaaaga gttttcagat ttcttctcag tacttataaa ccggtcatga actggtcgtg
     1741 cgatcacaaa gcgctggtct tgggtgtttc atttagtacg ttgattttag cattcattat
     1801 tttcggcagg atgggaggcg aatttgttcc cacgctcgat gaaggcgatt ttgttattca
     1861 gccggtgctc aaaaccggta cttcgctctc caaaaccgtg gaactgacca cgcagatgga
     1921 gaatattctg atagataaat ttcccgaagt ggaaaaaata gtgtgccgca ttggtgcagc
     1981 cgaagtgcct accgacccca tgtcgatgga agaaattgac atgattatca agctgaaacc
     2041 caaaaaagag tggaaatcgg ccgataccaa agaaggcctg gccgataaat tcaaagaggc
     2101 attgtcggtt attccgggta tcgattacga atttacgcag cctatcgaaa tgcgtttcaa
     2161 cgaactgatt acgggcgttc gctccgatat tgcggtgaaa gttttcggcg aagacctgga
     2221 ttatctcaac gaaaaagcct ctgaaattaa gcaattgata gaaggaattc ccggtgcagc
     2281 cgacgtcatt ctcgaaaaaa caacgggact tccgcaaatg aatgtgaagt acaaccgcga
     2341 aaaggtggct ttttacggcg tggatatgaa aacgatgaac gcctatcttg cagctgcttt
     2401 tggaggcgaa attgccggca gcattttcga aggtgaaaag cgttttgatc tggtggtaag
     2461 gctgcaaaag gaaaatcgta ccgacattga aaatatccgg cagcttcagg tgccgctgcc
     2521 taacgggatg caggttccgc tcagcgaact ggccgaaatt cgtctcaccg aaggaccggc
     2581 taaaatttcg cgtgaaaata ctcaccgccg cgtagtggta agtgtgaatg tgcgcaaccg
     2641 cgacctgcaa tcggtggtga aagatattca ggccagcgtg gataaaaatg tgaaactcaa
     2701 ccccgggacg tatattcagt atggcggaca gtttgagaat ttgcagaatg ccaccaaaag
     2761 actgattttt gccgttccga ttgcactggc gcttattttt attttcctcc atttcgcttt
     2821 caaatcgctc aaggatacca ttatgatatt cacggctgtt ccgctttctg ttgttggtgg
     2881 tgtgtttgct ttgtggctgc ggggaatgcc gttcagcatt tcggccggag ttggttttat
     2941 cgccctcttt ggtattgccg tgctcaatgg cattgtgctg attgagcacc tcaagcacct
     3001 caaagaaacc ggaatgacca atatgcgtga actggtgatg aaaggaacgg tcgatcggct
     3061 gcgtcctgta atgctcacag ccgcttctgc tgccatgggt ttcctcccga tggccatttc
     3121 caccagttcg ggagccgaag tgcagcggcc gcttgccact gtggttatcg gcggacttat
     3181 tacttctaca ttgctcacta tgattgcatt accgctgtta ttcatgattt tcaacgacga
     3241 ggaaggattg aaatacaaat ttgtccggaa atttaaattg aaaaattttc taatccctct
     3301 tgcattgatg ctttcgggag gtgcgctgat ggctcaaagt tcctcgctga cgctggaaca
     3361 ggccattgac ctggcactga aaaacaacag ggaaatcggg gcttacaatt tgaaagtgga
     3421 agaacgtaaa gcattggcta aaagcctgtt ggaaatggac aaaacggcgg tttactacgg
     3481 ctacgaccag gccaatgtgg ctgaaaacgg tcatccgatc cggacagtgg gagtggagca
     3541 gaattttttg tttcccacca ttcacgctcc gcgaaataaa gcccggaaaa ttgaaatttc
     3601 catggccgaa acggaacttc agaagcaaat gctgctgttg gtgaaaaatg tgaccggaac
     3661 ttactacacc attcagtttc tgcaggccaa aatcaaacat tatcagaaaa tggacagttt
     3721 gtacagcgtg ttgaatacgg gtgctgaaac ccgctacaag ctgggcgata tcagtcagct
     3781 cgatttgatg aatgcgcagg caaaaaagca acaggtaagc cttgaactca atcagatcaa
     3841 ctacgacatt cagaatacct accgaacgct gaatgcactt atgcagacca gtgaaactta
     3901 cactgtcgct tcggatgaga tgagtgtaat tcctgtcgta gaacccgatt ttcagcagaa
     3961 tgccgatgtc cggttgctcc ggttgcagtc tgattatatt aaaacaaatg tcaagaccga
     4021 acagcagcgt ttgttacccg atatttcgct gggatatttc tacgggaata accggtttga
     4081 aaataaccgg ggatatcacg gatttcaggt tggactgggc attccgctgt atgccggaga
     4141 gcaaaatgca aaaattaaag ccggaaaaat agcagcgaat accaccgaac tgctcctgca
     4201 aaacgacttg ttgttgctcg aaacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnataaca tatcagcccc ctaaatcact gccccctaac cccctaaagg gggaataaga
     4381 atgggacttt cagattatat tgtttattgc ttgttttata aaaagtgatt ttttataaaa
     4441 gtattttgat tttaaaatgt aaaatttttt ggattatgaa agacctgaaa cgaatatcat
     4501 tgattgcatt agcgcttgta ctcctggctg cctgcggcgg gaaaaaagcc actgataaaa
     4561 cagaaaatgc tccgaaagag gaattgatag aactaactca ggaacagttc agtaccagcg
     4621 gaatggaact cggagagcct tctatacaga gttttcgcga cgaagtggcc tgcaaaggct
     4681 acatcatagc accgcccaat tcaatggcca aagtgagccc accaataccc ggtgtggtaa
     4741 atgctatccg ttgtaagacc ggcgattttg ttcgggccgg acaggtggtt tgcaccattt
     4801 cggggaatga atttctgagc ctgcagcagg attttgccga aacggcagcc cgtttctcca
     4861 aatctaaact ggattatgaa cgtgccaagg ccctgagaga agaaaaaatt ggttcggaaa
     4921 aagattttat agccgtgcag agcgaataca aaactgtttt agccagttac aacgccctta
     4981 aagtgaaaat aaatgccatc cggctggatg ctgcccggat agaaaacggg caaatgtaca
     5041 cctcatttcc ggtggtttcg cccatcagcg gctatgtaac caaaacgagt gccgtcatcg
     5101 gtcagtatgc cgatatgcag aatgaacttg tggaaatagt gaatgtcaac ggattgcaac
     5161 ttcagctggc agtgttcgaa acggatattc agaaattgag agcaggtcaa gcggtgcatt
     5221 tcggaatatc cggtagcact tccaaatcgc tcggtgctac gctcattacc gtgggcaaaa
     5281 ccatcaatcc cgacaccaaa gtcattgact gcattgcccg tatagaccgc gcaagcgatg
     5341 cacaactcgt taatcagagt tttgtagaag ccacagtcat tgtgaataat aaatcggcca
     5401 aagcattgcc ggtgagtgcc gtgcagaagg tggataaagc gtattttatt tatgtgctgg
     5461 aacgaaagga aggcgaaaaa ttcctgctga agaaaacacc cgtcgaagtt ggtgcc
//
LOCUS       DAOPZK010000127         5301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_19614_length_5301_cov_28.462638, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000127 DAOPZK010000000
VERSION     DAOPZK010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19614_length_5301_cov_28.462638"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PKH68_07495"
     CDS             <1..264
                     /locus_tag="PKH68_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12819.1"
                     /translation="WKERRYLKRMITEAKRLHLITKKQYHVVPLEGGKLDIVDNHFLK
                     SYNRIDKSGRITGIELRKMALYSTPLDGLLLKLEAMKHRKATK"
     gene            321..602
                     /locus_tag="PKH68_07500"
     CDS             321..602
                     /locus_tag="PKH68_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNX12820.1"
                     /translation="MEERPRSADEYHTARWTRESRDFRRLHPLCQRCESKGIIRASEV
                     TDHIVPSEVHGDFWDRKNWQALCKRCNIEKGNEDKKLIQQFRNENRKDN"
     gene            580..1533
                     /locus_tag="PKH68_07505"
     CDS             580..1533
                     /locus_tag="PKH68_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNX12821.1"
                     /translation="MKTEKTINQLITEFVSEKDVREQTRKQYQTNLNQWFRWLNLQKI
                     DVRNPRRADAIAFKDYLNKTKSTYTIASYVTVLKLFYKWLEDQGIYENIASTVKFPKR
                     SDQHTKGYLKQDQVIDMLAAMPLRTISDFRNYAIVNLMLRTGMRRCEVVRMNVEDLII
                     SNDVYQLHIQRKGRLSKDQTITLDKAIVDPIREYLSLRAPFADSSPVFINHSRFNINQ
                     RINEDYISRLAKESMRRIGIERKDMTCHSFRHTAAITAIKAKALPYEVQQMLGHRSGS
                     TTEIYFKAAEAETMSNNPAISAIALSYQKQTKTVQKQAVSL"
     gene            1765..2337
                     /locus_tag="PKH68_07510"
     CDS             1765..2337
                     /locus_tag="PKH68_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNX12822.1"
                     /translation="MKNQNISQKEKTENKMLKKITKHNFKFKNKIENDCEVIKQLVSE
                     LQPDEILNLISKEFDSPNIVLSFVNQIERLFVATWAITPAGIDALVKIASNEIIKECW
                     IILDKTHSYKWIFQSDAYKILRGKVKIKFLANHSKFICFQLSSGEMVNFVGSMNFSRN
                     PRFENITIDKTQSNFEFYRDFIKNIGGEIV"
     gene            2334..2834
                     /locus_tag="PKH68_07515"
     CDS             2334..2834
                     /locus_tag="PKH68_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HNX12823.1"
                     /translation="MNAGRKSLPNEIKKLRGTDQPCRVKETIDVPKVGDISQVINVSK
                     LKVLKTKRAKQIFKEKANQLIALQILTEIDFEQLAIYAHSLDTLFDCIDNITASGKFS
                     EVHDENGRILRFIQNPYLKLYQDMVQICSKIGSEFGFSPVSRMKLHAKPEEKDPLQEL
                     MKQFGG"
     gene            2835..4526
                     /locus_tag="PKH68_07520"
     CDS             2835..4526
                     /locus_tag="PKH68_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HNX12824.1"
                     /translation="MNPKNYIKTVEKYISDVLSGEKIAGKLEILAVKRHLSDIENAHE
                     RKIFFDDKAARKAFAFFSLLKHSKGVFFNKPFDLSPWQAFIVYSLFGWKRADGSRRFR
                     YAYVEVARKNGKTTFAAAIALLMMVFDGERGAEIYFAATKEKQAKICWDEAKNMVGTS
                     GLSKYITVFRSSNTLVMDSTLSKMEPLSGDSKVQDGLNVHFAVCDEKHAWKTDELYNV
                     LTSATGARRQPMIFTITTAGFNKSYPCFDERKVNVEILEGVKQQDNTFVMIFTLDPGD
                     DYNDQRLWQKANPNIGISTNREYIFEEYEIARNHRGTKLTNFLTKLLNIWVDAPTVWI
                     PDEKVAACSYDTTEEMLYGKECYSGLDLASHVDVNALAHFFPNVNGHPVAKLFFWVPE
                     AKIQENADRVDYRLWREQKFMFSTPGDLIDTEWITNGILEVTKKYKCLGLAYDPYKAH
                     GGIVQGLIKGGMERIIDEYSQSIRSMSEPTKELERMVTGAEIDLMNNPVLRWMFRNVT
                     IYTDPNNNIKLDKSKSQNKIDGVVALAMAIGEYMTKTAGKKQDINKWVPRSMPSL"
     gene            4523..4714
                     /locus_tag="PKH68_07525"
     CDS             4523..4714
                     /locus_tag="PKH68_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12825.1"
                     /translation="MMSERYIQMLDKAGFCSLFWEKWKTGDYARHEDAYESLETEYIE
                     FFGCRKYKDFKSFRRRRDE"
     gene            4781..5299
                     /locus_tag="PKH68_07530"
     CDS             4781..5299
                     /locus_tag="PKH68_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12826.1"
                     /translation="MGVKGTRKNQTATDVYTGLATVRKKPDTDTHRELCQRGGKWLTK
                     HSQNVLVPNCNIVAVELFTQVEPEIPDIIGWCSWCSIMIEVKVQRSDFLQDFKKPFRQ
                     ICADGVGELRFYLCPENMISESELPENWGLLYETKRGIKIIRKAERQSTNLAAERNML
                     TSYIRNKTQHCW"
BASE COUNT         1808 a         1062 c         1088 g         1343 t
ORIGIN      
        1 tggaaagaac gccgatacct gaaacgtatg attactgaag cgaaacgttt gcatctgatt
       61 acaaaaaaac aatatcacgt tgtaccgctt gaaggtggaa agcttgatat tgttgataac
      121 cattttttga aaagctacaa cagaattgat aaatcaggac gtattaccgg aattgagttg
      181 cgaaaaatgg ctttgtactc taccccgctt gacgggctgc tactgaaact ggaggcaatg
      241 aaacatcgaa aagcaacaaa gtaatgcaaa agcaacgatt aattaggccg tggattccgc
      301 aacataaccg caccacaaaa atggaagaaa ggccgcgaag tgccgacgaa taccatacag
      361 cgcgatggac gcgcgaaagc cgggattttc gccgcttgca tccgctttgc caaaggtgcg
      421 aatcgaaagg tatcatccgc gccagcgaag taaccgatca cattgttcct tctgaagttc
      481 acggcgactt ctgggaccgt aaaaactggc aggcattatg taaacggtgc aacattgaga
      541 agggaaatga agataagaaa ttgattcaac aattcaggaa tgaaaaccga aaagacaatt
      601 aatcaactga ttactgaatt tgtatcagaa aaagatgttc gcgaacaaac aagaaaacaa
      661 tatcaaacaa acctaaatca atggttcagg tggttgaatt tgcaaaaaat cgatgttcgt
      721 aacccgcgcc gtgccgatgc cattgccttt aaagattacc tgaataaaac aaagtcaaca
      781 tacaccattg catcgtatgt aactgttttg aaactgtttt ataagtggct ggaagatcag
      841 ggaatttacg aaaatattgc gtcaactgtt aagtttccaa agcgatcaga tcaacataca
      901 aaaggctacc tgaaacaaga tcaggttatt gatatgctgg ccgccatgcc attgcgaact
      961 atcagcgatt ttaggaacta tgcaatcgtt aacctgatgc ttcgcaccgg gatgcgacgt
     1021 tgtgaagttg taagaatgaa tgtagaggat ttgataatca gtaatgatgt gtatcagtta
     1081 catatccagc gaaaaggaag attatcgaaa gaccaaacca ttacgcttga taaagcaata
     1141 gttgatccaa ttcgcgaata cttgtcactt cgcgctccgt ttgctgatag ttctccggta
     1201 ttcatcaacc attcacggtt caatatcaat cagcgaatca atgaggatta catatcaagg
     1261 ctcgcaaaag aatccatgcg ccggataggt attgaacgca aagatatgac ctgtcattca
     1321 ttcagacaca ccgccgcaat tacagctatc aaagctaaag cattgccgta cgaagttcag
     1381 caaatgttag ggcaccgaag cggctcaact actgaaatct attttaaagc cgctgaagcc
     1441 gaaacgatga gcaataaccc tgctataagc gcaatagcct tatcttatca gaaacaaacg
     1501 aaaaccgtac aaaaacaagc tgtttcgctt taatatgtct ataaggttca attaatagag
     1561 aattatacga acctattcta ttgaataatg tgattattaa aagttagtaa tatcaatcat
     1621 gcaacgcttt gagggatagg gggtaaaaat ctctgagtca aatatgcgta agaccactac
     1681 cctatcaggt tacagacgca tgcaaaatta ccaaatatga gccattttag aaatcagttg
     1741 aatttagact ttagtggagt ttcaatgaaa aatcaaaata ttagtcagaa agaaaagact
     1801 gaaaataaga tgctgaagaa aattacaaag cataatttca agttcaaaaa caaaattgaa
     1861 aacgattgcg aagtaataaa gcagcttgtt tcagaacttc aacctgatga gattctgaat
     1921 ttaatcagca aagaatttga ttcgcctaat atagttctgt catttgtcaa tcagatcgaa
     1981 agattgtttg tcgcaacgtg ggccatcact cctgccggaa ttgacgcgct tgtgaaaatc
     2041 gcttcaaacg aaataataaa agaatgctgg ataatacttg acaaaacaca ttcgtacaaa
     2101 tggatatttc aatcagatgc ttacaagatt ttgcgcggaa aagtaaaaat caagttttta
     2161 gccaaccatt caaaattcat ttgctttcaa ttgagttcag gcgaaatggt aaactttgtt
     2221 ggctccatga atttcagcag aaatccacgc ttcgaaaata taacgataga caaaactcag
     2281 tcaaatttcg agttttaccg cgactttatt aaaaatattg gaggcgaaat cgtatgaatg
     2341 ctggccgcaa atcattacca aacgaaatca agaaactacg cggaaccgat cagccttgcc
     2401 gagtaaagga aactattgat gttccaaaag tcggtgacat ttcacaggtt attaatgttt
     2461 caaaattgaa ggttctcaaa acaaagcgtg caaagcagat ttttaaagaa aaggcaaacc
     2521 agcttattgc gcttcagatt ttaactgaaa tagattttga acagcttgca atttatgccc
     2581 attcgctcga cacccttttc gattgcattg ataatatcac agcatcaggt aaattttcag
     2641 aagtacacga tgaaaatggg cgtatccttc ggtttatcca gaatccttat ctaaaacttt
     2701 atcaagatat ggtgcaaatc tgttcaaaaa tcggttccga atttggtttt tcgcctgttt
     2761 cccgcatgaa attacacgca aaaccagagg aaaaagaccc tttgcaagaa cttatgaagc
     2821 aatttggagg ataaatgaac ccgaaaaact acatcaaaac agtcgaaaaa tacatttcag
     2881 atgtgctttc aggcgaaaaa attgccggaa aattggagat tttagccgtc aaacggcatc
     2941 tttccgacat agaaaatgct cacgaacgca aaattttctt cgacgataag gcagcacgta
     3001 aagcatttgc ctttttttca ctgctaaaac acagcaaagg agtgtttttt aacaagcctt
     3061 tcgatctttc cccatggcaa gcgttcatcg tttattcact tttcgggtgg aaacgcgccg
     3121 acggatcacg ccggtttaga tatgcctatg ttgaagttgc cagaaaaaac ggaaaaacaa
     3181 cttttgccgc tgcaatcgct ttgctcatga tggttttcga cggcgagcgg ggagccgaaa
     3241 tctattttgc ggccaccaaa gaaaaacagg caaagatttg ctgggacgaa gcaaaaaaca
     3301 tggttggcac ttcaggcctt tcaaaataca ttactgtttt ccgaagttca aacacactgg
     3361 tcatggactc cacgctttca aaaatggaac cgctttctgg cgactcaaaa gttcaggacg
     3421 ggttgaacgt tcacttcgct gtttgcgacg aaaaacacgc ttggaaaacc gacgagcttt
     3481 acaacgtgct cacatcggcc accggcgccc gccgccagcc aatgattttc accataacca
     3541 ccgccggatt caacaaatcg tacccatgtt ttgacgagcg caaagtaaac gtcgaaattt
     3601 tagaaggtgt caaacaacaa gacaatactt tcgtcatgat attcacgcta gatcctggcg
     3661 acgattacaa tgatcagcgc ctttggcaaa aggcaaaccc aaacatcggc atttcaacca
     3721 atcgcgaata tatttttgaa gaatacgaaa tcgcccgaaa tcacaggggg accaaactaa
     3781 ccaatttcct tacaaaactc ctgaatattt gggttgatgc tcctacggtt tggattccag
     3841 atgaaaaagt tgctgcctgc tcgtacgata cgactgaaga aatgctttac ggtaaagaat
     3901 gttattcagg tctcgacctc gcaagccatg tagatgttaa tgccttggct cattttttcc
     3961 cgaacgtcaa tggccatccg gtggctaaac ttttcttttg ggttcctgaa gccaaaattc
     4021 aggaaaacgc cgaccgtgtc gattaccgtc tctggcgcga gcaaaaattc atgttctcaa
     4081 cgcccggtga tttgatcgat accgaatgga tcactaacgg aattctcgaa gtaaccaaaa
     4141 aatacaagtg tttggggttg gcttacgacc cttacaaagc tcatggcggc attgttcagg
     4201 gtctaatcaa aggaggtatg gaaagaatta tcgacgagta ttcgcaatct atccgttcaa
     4261 tgtccgaacc aaccaaagaa cttgaacgca tggttacggg tgccgaaatc gacctgatga
     4321 ataaccccgt tttgcgctgg atgttccgaa atgtaaccat ttacaccgac ccaaataata
     4381 acattaagct cgataagtca aaatcacaga ataaaattga cggagttgtt gctctggcaa
     4441 tggcaatcgg tgagtatatg accaaaacag cgggcaaaaa acaagatatt aacaagtggg
     4501 ttccacgttc aatgccttca ttatgatgtc agaacgatac attcaaatgc tagataaagc
     4561 tggtttttgc tcactttttt gggaaaaatg gaaaaccgga gactatgccc gccacgaaga
     4621 tgcttacgaa tcgctcgaaa ctgaatacat cgagtttttt ggatgccgaa aatacaagga
     4681 tttcaaaagc ttcagaagac gaagggacga ataacgaaaa agtatatgcg gtcggctgcg
     4741 atgcaaagct ccaccgattc aaattagtaa taacttagat atgggagtaa aaggaacaag
     4801 aaagaaccaa accgcaactg acgtatatac ggggttagca actgtgcgga aaaaacccga
     4861 cacagacacc cacagagaac tatgccagag aggcggtaaa tggcttacta aacattcgca
     4921 aaacgtatta gtgcctaatt gcaacattgt ggcagttgaa ctttttacac aagtagaacc
     4981 agaaataccc gatattattg gttggtgcag ttggtgttca attatgatag aagtaaaagt
     5041 tcaacggtca gattttctac aagatttcaa aaagccattt agacaaattt gtgctgatgg
     5101 agtaggtgaa ttacgttttt acttatgtcc tgaaaacatg attagtgaaa gtgaattgcc
     5161 tgaaaattgg ggattgcttt acgaaactaa acgaggaatt aaaattataa ggaaagcaga
     5221 aaggcaaagc acaaatttgg cagcagaacg gaatatgctg acaagctaca ttaggaataa
     5281 gacgcagcat tgttggtaac g
//
LOCUS       DAOPZK010000128         5291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_19663_length_5291_cov_21.532468, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000128 DAOPZK010000000
VERSION     DAOPZK010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19663_length_5291_cov_21.532468"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..516
                     /locus_tag="PKH68_07535"
     CDS             103..516
                     /locus_tag="PKH68_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNX12827.1"
                     /translation="MKLSKTSEYALRILIFMAKNPEELYTAKYLIEKLNVSDKYLRRL
                     MTDLSKSGFIRSTQGRDGGYTFAKDIHDIFLYDIIDSVEGMEQLNDCVLGFNECSCSN
                     PCAMHDDWIIIREKLNKVFNQTKLSDLEFDKILRY"
     gene            704..1066
                     /locus_tag="PKH68_07540"
     CDS             704..1066
                     /locus_tag="PKH68_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNX12828.1"
                     /translation="MKKISYNELVTTQNPHGVDARKLHANEDVQVVHMHLKPGQSLKK
                     HTTAVNVFFYVLEGEGVVEIGDEKQTVTKDCLIDSPKDIPHCLYNESDADFRVLVVKT
                     PSPTAAQNRQAIENMLKN"
     gene            1078..1623
                     /locus_tag="PKH68_07545"
     CDS             1078..1623
                     /locus_tag="PKH68_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNX12829.1"
                     /translation="MSAIKILVGEHDVILRMIDVTRRLLADTTTVNIGHVEQILDFIK
                     NFADKYHHLKEEDVLFLEMEHHGMSRQSGPVGVMLHEHEQGRAYVKHAEEAILKFKSG
                     NSQAVNEIKDNLLNYCALLTNHIYKENNILYPMAERLLPDAVMQAMSEHFDVAITATV
                     NNEYADKYLKIAEELSRIYMK"
     gene            1644..2339
                     /gene="ric"
                     /locus_tag="PKH68_07550"
     CDS             1644..2339
                     /gene="ric"
                     /locus_tag="PKH68_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNX12830.1"
                     /translation="MKTYKQTKIGDIVTQDFRAAEVFKKAGIDFCCGGSQSLEAACRD
                     KKLDVAEIESELEKLENSEPGSSHKFNEWKLDFLFDYIVNTHHQTVMKLLPELTFYTQ
                     KIAEVHGDNHPELSEIANLFAQVDTELRQHLRNEEEVLFPAIREVLKTNSAESKATII
                     SEITRMTGEHEFAGGAMDKINELSHRYAVPEDGCNTYRVAYKLLEQFEDDLHIHVHLE
                     NNILYPKAMKLPV"
     gene            2496..3734
                     /locus_tag="PKH68_07555"
     CDS             2496..3734
                     /locus_tag="PKH68_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNX12831.1"
                     /translation="MSELINNSQSRKDKLKSLILKLHNGETQESVRNELLLSLSRIPY
                     GEVVEVEQELIAEGLPEEEILDLCDAHSSVLEGRVDLSALKPVPDGHPVDVFRKENEE
                     IRNVTNAIFALISDILHSSSESVREQVIKLRGLYNNLYDVDKHYQRKEYLLFPYLEKL
                     EITGPPKVMWGKHDEIRELIKGSIEVLQVDSLTREELEAAAEMVLKPAVRSAQEMITK
                     EEEILFPMALDTLNESDWYEISRQSIEIGFCLYDPPVDWRPEWVAEQSINESQKSGSH
                     IQLPSGSFSAEELLAILNTLPVDMTFVDKDDKVKYFSQGAERIFQRNRAILNRDVRHC
                     HPPASAHIVDKIIEDFKTGRENRAPFWINMGGKKMIHIEYFALRNEKGEYLGTLEVSQ
                     NVQPYRDLEDEQRILSYSKK"
     gene            3738..4262
                     /locus_tag="PKH68_07560"
     CDS             3738..4262
                     /locus_tag="PKH68_07560"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNX12832.1"
                     /translation="MMETPKLIISPKTKVLQLIETYPQLEELLISYVPAFRKLKNPVL
                     RNTVAKITTLQQAAVIGNVKVEELINLFRSEVGEDLIEMAEDSTFNTIQPDWYDEDRI
                     VSSLDIRAMLNVGEQPVNQVITDLNKLKPAEIYEVVAPFIPAPLIDKATSLNMVHWID
                     QKGAELFQVYFCRK"
     gene            4661..5074
                     /locus_tag="PKH68_07565"
     CDS             4661..5074
                     /locus_tag="PKH68_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12833.1"
                     /translation="MKKLLLLPFLIAFLFSSCEPVPEDLTTKVIYNFTINGREWVAHT
                     DDAGLNRYYSCSVSVRDLKSLVYEKGAVIAYMLMDGYQLTLPYIRHYENSKGERWTRT
                     VDFDFSDGVINFYVTNSDFAVDPPGTVDFRVILMW"
BASE COUNT         1773 a         1059 c         1112 g         1347 t
ORIGIN      
        1 aacaggtgaa tttcataaaa aaagataaaa tcgatcggat ttttgctaat ccgagaaaaa
       61 tgtttttcct ttgcagaaaa taatacaaca aggtagtatt taatgaaatt atcaaaaaca
      121 tcagagtacg cattgcgcat attgatcttc atggccaaaa accctgaaga attatatact
      181 gccaaatacc tgatcgaaaa gttgaatgta tctgataaat acctccgcag attaatgacg
      241 gacctgtcaa aatcaggctt tatcaggagt acgcagggac gggatggcgg atatacattt
      301 gctaaagaca ttcatgatat ttttttgtac gatattatcg actcggtgga aggcatggag
      361 cagctgaacg attgtgtgtt gggtttcaat gaatgttcgt gctcaaatcc ctgtgccatg
      421 cacgacgact ggattattat aagagaaaag cttaacaagg tttttaatca aaccaaattg
      481 tcggatttgg aatttgacaa gatattacga tattagtttt tttgtaatta tatgctactc
      541 cgaagtagta tataattcgc aactataaat gatgaaaatt caataagcat aaaaacacaa
      601 aaaatagaac agacaaagtg ataattataa ttactaacca aacttctgca cccttcttat
      661 ccgccctttc agggttatgg ggcagataaa aaaaataaaa aaaatgaaaa aaatcagcta
      721 caacgaactg gtcactaccc aaaaccctca cggtgtggat gcccgaaaac tacatgcaaa
      781 cgaagatgtt caggttgttc atatgcacct taaacccgga caaagtctga aaaaacacac
      841 cactgcggtg aatgtatttt tctatgtact ggaaggtgaa ggtgtggtag aaataggaga
      901 tgagaagcaa accgtaacaa aagactgtct catcgacagc ccgaaagaca tcccgcattg
      961 tctctacaac gaaagcgatg ccgacttccg tgtattggtg gtaaaaactc cttcgccaac
     1021 agccgcccaa aacagacaag ccattgagaa tatgttgaaa aattaacgaa aaggacaatg
     1081 agcgcaatta aaattctggt cggtgaacat gacgtcatcc tgcggatgat tgatgttaca
     1141 cgccgtctgt tggccgatac aacaacagtc aatatcggac acgtggagca aatcttagat
     1201 tttatcaaaa actttgccga caaataccat cacctgaaag aagaggatgt ccttttctta
     1261 gaaatggaac atcacggcat gagccggcaa agcggtcctg taggtgtcat gcttcacgaa
     1321 cacgaacagg gtagggcata cgtgaaacat gcagaagaag ccattttaaa attcaaatca
     1381 ggcaatagtc aggcagtcaa tgaaataaaa gataatttat tgaattactg cgccttactc
     1441 accaatcata tttacaaaga gaataatatc ctgtatccga tggccgaaag attgcttccg
     1501 gacgcagtaa tgcaggccat gtccgaacat ttcgatgtag ccattacagc caccgtgaat
     1561 aatgaatatg cagacaagta cctcaaaata gccgaagaac taagccggat ttatatgaaa
     1621 taattttcta taaaaacaac ataatgaaaa catacaaaca aacaaaaatt ggtgatatag
     1681 ttacgcagga tttcagagcc gccgaagtat tcaaaaaagc aggcattgat ttctgctgtg
     1741 gtggcagtca aagcctcgaa gctgcctgcc gcgacaagaa gctggatgtg gctgaaatag
     1801 aatcggaatt ggaaaaactc gaaaattctg aacccggttc gtcgcataag ttcaacgaat
     1861 ggaaactgga ttttctgttt gactacatag tgaatacaca ccatcaaacg gtgatgaaac
     1921 tgttgccgga actgaccttc tacacccaaa aaatagccga agttcacggc gataatcatc
     1981 ctgaattgtc cgaaattgcc aatctctttg cacaggtgga cacagaactg cgtcagcacc
     2041 ttcgtaatga agaagaagtg cttttccctg ccatccggga agtgttgaaa accaattcgg
     2101 ccgaatcaaa agccaccatc atcagcgaaa taacaagaat gaccggtgaa catgaatttg
     2161 caggaggtgc catggacaaa atcaatgagt tgagccaccg ctatgcggtg cccgaagatg
     2221 gctgcaatac ctaccgcgtg gcatacaagc tgctcgaaca atttgaagat gatctgcata
     2281 tccacgttca tcttgaaaat aacattctgt atcccaaagc gatgaaactg ccggtgtgaa
     2341 tgtaccccaa actgcagaaa atgaagatag tgaaggggtc ttaaaacaga acattaccga
     2401 aaacataaac aggtaaataa tcgttttaat ttttcaagga tcaatttctt caaaaaaaac
     2461 tgagtaaaaa tcaatttaca aaaaaaaata aaattatgag tgaattaata aacaattccc
     2521 aaagcagaaa agacaaatta aaatcgctga ttctgaaact tcacaacggt gaaacccagg
     2581 aatctgtgcg caacgaactg ctcttgtcgc tcagccggat accgtatggc gaagtagtgg
     2641 aagtagagca ggaactgata gccgaaggac tgcctgaaga ggaaatactg gacctgtgtg
     2701 atgctcactc atcggtactc gaaggacgcg ttgacttgtc ggcactcaaa ccagtacccg
     2761 acgggcatcc ggtagatgta ttccgcaagg aaaatgaaga aatacgaaat gtaacaaacg
     2821 ccatttttgc actgatatca gatatacttc acagcagcag tgaatctgtc cgggagcagg
     2881 tgattaagct gcgcggatta tacaacaatc tgtacgatgt ggataaacat tatcagcgta
     2941 aagaatacct cctttttccg tatctggaaa aactcgaaat aaccggtcca cccaaagtaa
     3001 tgtggggtaa gcacgacgag atacgcgaac taatcaaagg aagcattgaa gtgctgcagg
     3061 tggacagcct gaccagagaa gaactcgaag cagctgcgga aatggtgctg aaacccgctg
     3121 tgcgtagtgc acaggagatg attacgaaag aggaggaaat tctcttcccg atggcgctcg
     3181 atactctcaa cgaaagcgac tggtacgaaa taagcagaca atccatcgaa ataggctttt
     3241 gcttgtatga tcctcctgtg gactggcgcc ctgaatgggt ggccgaacaa tcaatcaacg
     3301 aatcgcaaaa aagcggaagc catatacagt tgccgagtgg aagtttctcg gccgaagagc
     3361 tgctggctat cttaaataca ttgccggtgg atatgacttt tgttgataag gatgacaaag
     3421 tgaaatactt ctcgcagggt gcagaacgta tttttcagcg taaccgcgcc atcctcaacc
     3481 gcgatgttcg tcactgtcat ccgcctgcaa gtgctcacat agttgataaa attatcgaag
     3541 atttcaaaac aggcagagaa aaccgtgccc ccttctggat caacatgggt ggaaaaaaaa
     3601 tgatccatat cgagtatttt gctctcagaa atgaaaaggg cgaatacctg ggaacgctgg
     3661 aagtatcaca gaatgtacag ccctacagag acctggaaga tgaacagcga attttatcct
     3721 attcaaaaaa ataaaaaatg atggaaacac caaaactgat cataagtcct aaaaccaaag
     3781 ttctacagct tatcgaaaca tatccgcaac tggaagagtt attaatcagt tacgttcctg
     3841 ctttcaggaa actgaaaaat ccggtattgc gaaacaccgt tgctaaaatt accacgttgc
     3901 agcaagctgc agtgatagga aatgtaaaag tggaagagct gattaatttg tttcgcagtg
     3961 aagtggggga ggatttgata gaaatggcag aagatagcac tttcaatacc attcagcccg
     4021 attggtacga cgaagaccgg attgtcagct cgctcgatat cagagctatg ctcaatgtgg
     4081 gtgaacaacc tgtaaatcag gtaatcactg atctgaacaa actgaaaccg gcggaaatat
     4141 atgaagttgt tgcaccgttt atccctgctc cgcttattga caaggctacc agtctgaata
     4201 tggtgcactg gatcgaccaa aaaggcgctg aactgtttca ggtttatttt tgcagaaaat
     4261 aaagacacca tcgggctgca agctggtcaa ttattttaag gcggtttcgg aaatgatttc
     4321 ggaaccgcct tgtcttttgt tactgtctcc ttaaaatgac gcgatttgca tatgatttcg
     4381 tgaaaagttt tacatcagtc aaattctttt tttcttttaa aaaaaatccc tttaacttct
     4441 ttcataaagt aggatcattt gtaaagacaa gtcaaaacag atcaatgcgc acatcaaaaa
     4501 aactgcccat tctcccgacc agcggatttt ttttaacaaa ttagcaaagt ctggcaatta
     4561 actcaaataa tttcatactt ttgcacagtc catccggatt gtaacagcct gactgacgct
     4621 aaaactaaca tttttactca tacagaaaaa cacaaattca atgaaaaaac tactcttgct
     4681 tccttttctg attgcattcc tgttctcatc gtgcgaaccg gtccccgaag acctgactac
     4741 caaagtgatt tataatttca ctatcaatgg cagggagtgg gtagcacata cagatgatgc
     4801 cggactgaac cgttactatt cctgcagtgt atcagtcagg gatttgaaaa gtctggttta
     4861 tgaaaaaggg gcagtgatag cttatatgct gatggatggt tatcagctga cattacccta
     4921 tattcgtcat tacgaaaaca gcaagggtga aagatggacg cgcaccgttg attttgattt
     4981 ttcggatgga gtgattaatt tctatgtaac caattccgat tttgcagttg atcctccggg
     5041 cactgtggat tttcgcgtta tcctgatgtg gtaggcagat aggttacttt gaacttcctt
     5101 ttatctcgat agcaacattt accgggataa ctttcacttc tgtcatatct gttgtttctt
     5161 ttgtgaaatg aaaaaatgga agaaaagaca aaaaagtccg caatgaaatt cactgcggac
     5221 tttttagggt tgatgaatta taatttcgag aatttttgtg tagaaacccc gccatttttc
     5281 attttggcca c
//
LOCUS       DAOPZK010000129         5290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_19668_length_5290_cov_25.495511, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000129 DAOPZK010000000
VERSION     DAOPZK010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19668_length_5290_cov_25.495511"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKH68_07570"
     CDS             complement(<1..677)
                     /locus_tag="PKH68_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNX12834.1"
                     /translation="MKKLYFNLLLMSVSLTFNSAKAAEPYMNTSLSFEERTNDLVSKL
                     TLEEKVQLMRYDSPEINRLGIPAYNWWNECLHGVARSGLATVFPQAIGMAAMWDDTMM
                     FNIADAISDEARAKYNDYTRRDKRGIYQGLTFWTPNINIFRDPRWGRGMETYGEDPYL
                     TAGMAIPFIKGLQGNDPKYLKLVATAKHFAVHSGPEATRHSFDVWPNDYDLAETYFPH
                     FKRTVTEA"
     gene            complement(772..1746)
                     /locus_tag="PKH68_07575"
     CDS             complement(772..1746)
                     /locus_tag="PKH68_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNX12835.1"
                     /translation="MKTPRYLVPNLYTADPAVHIFNGKIFIYPSHDVESGIPENDNGD
                     HFDMCDYHVFSMESIDGEVTDHGVVLDVKSIPWAGRQLWDSDAAYKDGKYYIYFSLKD
                     KNDVFHIGIAISDRPEGPFTAFDNPIKGSYSIDPCVFREDDGSHFMCFGGLWGGQLQR
                     YRNNKAIECGHEPANNEPALCAKIVKLSDDMLDFAEEPRDLVILDENGKPLLAGDHDR
                     RFFEASWMHKYNGKYYFSYSTGNTHLLNYATSDNLYGPFTYRGVILTPVVGWTTHHCI
                     TEFDGKWYLFHHDCVPSGGKTWLRSLKVVEMEYNADGTIKTIEGTAVR"
     gene            complement(1965..3137)
                     /locus_tag="PKH68_07580"
     CDS             complement(1965..3137)
                     /locus_tag="PKH68_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNX12836.1"
                     /translation="MNVISKLRTFVVLIVVCFLSVQCAPKSQLTLKEVLKDKFYIGTA
                     MNTDEINGVDTGSIRVIKQQFNAVVAENCMKSGVIQPEEDKFDFTLADKFVDFAEKNN
                     LYATGHVLIWHSQAPKWFFTDANGKDVSPEVLKQRMIKHISTLVGRYKGRVKGWDVVN
                     EAIEDDGSWRNSKFYQILGEDYIKLAFEYARDADPDAELYYNDYSMAHPGRRDAVVKM
                     VNKLKQEGIKVDGIGMQGHMTMDFPTCDAYEKSILAFASTGAKVMITEMDITVLPSPD
                     RYRGADVGKKFEFDKKMNPYPDGLPDSMATKLNERYASFFRLFLKHSDKISRVTLWGV
                     GDAQSWRNYWPIFGRTDYPLLFDRNLRPKPIVETIIQMAGKLNQPQQTSPKGKLKD"
     gene            complement(3234..4655)
                     /locus_tag="PKH68_07585"
     CDS             complement(3234..4655)
                     /locus_tag="PKH68_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX12837.1"
                     /translation="MESISKAKTNESRGFYKLTAIQRIGFGSGDLAQNLIYQTVAQYL
                     LIFYTNVFGLPAATAAVMFLIVRLVDVVWDPLVGAYVDKHNPKLGKYRTYLLLGGIPL
                     TGFAILCFWNGFSGSLLYAYITYVGLSMLYTLINVPYGALNASLTRDTDEITKLTSVR
                     MFLANLGGLAVAYGIPIVVKTLSPDGKINSPASGNAWFITMTIYSLAGLALLIFCYTQ
                     TKERVVMDVKETDNVKVSDLWNEFKYNRPLRVLAFFFITAFAMMAIGNSAGSYYMIYN
                     VQAPDWLPYFAALGSIPAFIFMPMIPAIKRAIGKKQMFYVFLSVAIFGMAMLYIISVL
                     PGLRQHLWLVLVAQFVKSTGVIVATGYMWALLPEVISYGEHTTGKRISGIVNALTGIF
                     FKAGMALGGVVPGFVLAFVNFDEKNTVSQTPFVQQGILWLVAVIPAILLMLAMFIISK
                     YELDDDKIDKINMEIEERYSTNS"
     gene            complement(4781..>5290)
                     /locus_tag="PKH68_07590"
     CDS             complement(4781..>5290)
                     /locus_tag="PKH68_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HNX12838.1"
                     /translation="KCTFDNRYISLEAEHYSRKNETGGIHWEIIPDLGKTLSGVTTFP
                     QNVYPKSTDAVYLEYDVNFEIAGEFPVHILVSPTLNFNDNKGLRYAVSFDGGTEQIVN
                     INQNYDVKLMEKWQEKSINETITRHKIDKPGKHSLRFRVLEPGIVLQKIMIDLGGLKK
                     SYLGAPGKR"
BASE COUNT         1510 a         1136 c         1069 g         1575 t
ORIGIN      
        1 gcttcggtta ccgtacgttt gaaatgtgga aaataggttt cagccaggtc gtaatcgtta
       61 ggccagacat caaaggaatg gcgggtggct tcgggtccgc tgtgaacggc aaaatgcttg
      121 gcagtagcta ccagtttcag gtatttgggg tcattgccct gcaagccttt gataaacggg
      181 attgccattc cggcagtgag gtacggatct tcaccgtaag tttccatgcc tctgccccaa
      241 cgcggatcgc gaaagatatt aatattgggt gtccagaaag tcaatccctg ataaattccc
      301 cgtttgtccc gcctcgtgta atcgttgtat ttagcccgtg cttcgtcaga aatggcatct
      361 gctatattga acatcattgt atcgtcccac atggcagcca tcccgatagc ctgaggaaaa
      421 accgtagcca aacccgaacg tgccacgccg tgcagacatt cgttccacca gttgtaggcg
      481 ggaatgccca accggttgat ttccggagaa tcatagcgca tcagctgcac tttttcttcc
      541 agcgtaagtt tcgaaaccaa atcgttggtg cgttcttcaa aactcaaaga ggtattcata
      601 tagggttctg ctgcctttgc agaattgaaa gtaagtgata cgctcattaa cagtagatta
      661 aaatataatt ttttcatttc taatcagttt gtaagattaa ttctaccagt tatccgttct
      721 gaaagtcgat acctgcaatc tttccttatt gaataaattg ggatgaaata atcaccggac
      781 tgctgttcct tcgatggttt taatcgttcc gtcagcgttg tattccattt ccaccacttt
      841 caggctgcgg agccaggttt ttccgcccga gggtacacaa tcatgatgaa aaagatacca
      901 ttttccatca aattccgtaa tacaatgatg tgtggtccaa cccacaaccg gtgtaagaat
      961 tactccccga taggtaaagg gtccatacag attatcactt gtagcataat tcagcaagtg
     1021 agtattgccc gttgagtaag aaaaataata tttcccgttg tatttatgca tccacgatgc
     1081 ttcgaaaaat cgtcggtcgt ggtcaccggc aaggagtggt tttccgtttt cgtccaaaat
     1141 aacgaggtca cggggttctt ccgcaaagtc aagcatatcg tcgctcagtt tcactatttt
     1201 agcacaaaga gccggctcat tattagccgg ttcgtgaccg cattctatgg ctttgttgtt
     1261 gcggtagcgt tgaagctgac cgccccaaag acctccaaaa cacataaagt ggctgccatc
     1321 atcttctctg aaaacgcagg gatcgatgga atagctgcct tttatcgggt tgtcgaatgc
     1381 ggtgaaagga ccttcaggcc tgtcactgat ggcaataccg atatgaaata cgtcgttttt
     1441 gtctttcaac gaaaaataaa tgtagtattt tccatccttg taagctgcat cggagtccca
     1501 caattggcga ccagcccagg gaatgctttt cacatcgagt actacgccgt gatcggtaac
     1561 ttcaccatcg atactttcca tcgaaaagac gtggtaatcg cacatatcga aatgatcgcc
     1621 gttgtcgttt tcaggtatgc cggattctac atcgtgtgat ggataaataa agattttgcc
     1681 attgaaaata tgcactgccg gatcggcagt gtaaagattt ggtacgagat aacgtggtgt
     1741 tttcataagt aaaaagttta acctccagcc ccctaaatcc cccgaagggg gacttaaagt
     1801 atgcttacag gcaaatattt tgttttataa aggtattatt aatatgtgtg ttttttttaa
     1861 aattttttgt atatatccac taaactgaac ccaagtctgt gctgtttttt ccttaatact
     1921 tgttaatata aattgaacgt gaatggaatg tttctatcgg aaaatcagtc tttaagcttc
     1981 cctttggggg aggtttgttg aggctggttt aatttgcctg ccatctgaat aattgtttcc
     2041 actattggtt ttggccggag attacggtcg aaaaggagtg gataatctgt gcgtccgaat
     2101 atgggccaat aatttcgcca ggattgtgca tctcctactc cccagagcgt tacacgactt
     2161 attttatcgc tgtgttttaa aaataatctg aaaaatgaag cataccgctc atttaatttc
     2221 gttgccatgc tatcaggcaa tccgtcaggg taagggttca tttttttatc aaattcgaat
     2281 tttttaccca catcagcacc ccggtatctg tcgggcgatg gaagaacggt aatatccatt
     2341 tcggtaatca tcactttagc gccggtagag gcaaatgcca gaatactttt ttcgtaggca
     2401 tcgcaggtcg gaaagtccat ggtcatgtgt ccctgcattc cgataccatc cactttaatg
     2461 ccctcttgtt tgagcttgtt caccattttc accaccgcat cgcggcgtcc gggatgtgcc
     2521 atggaataat cgttgtaata cagttcggca tcgggatcgg catcacgggc atattcgaaa
     2581 gcaagtttga tataatcttc acccaaaatc tggtaaaatt tgctgttgcg ccaggagcca
     2641 tcatcttcta tggcctcatt taccacatcc cagcctttta cacgtccctt gtagcgaccc
     2701 acgagtgtgg aaatgtgctt aatcatccgc tgtttgagta cttccgggct gacatctttt
     2761 ccattagcat ctgtgaaaaa ccactttgga gcctgtgagt gccagatgag cacatgtccg
     2821 gtagcgtaaa gattgttctt ttcggcaaaa tccacaaatt tgtcagccag cgtgaagtca
     2881 aatttgtctt cttcgggctg aataaccccg ctcttcatgc agttttcggc cacaacggcg
     2941 ttaaactgct gtttgataac ccgaatggag ccagtatcca ccccgttaat ctcatcggta
     3001 ttcattgccg taccgatata aaatttatct tttaatactt ccttcagcgt cagttgcgat
     3061 ttaggagcac actgtacgga caaaaaacaa acaacaatta gtacaacaaa tgttcgtaat
     3121 tttgaaatga cattcatgat cggtagttgt tttttatgtg taacattgag tttatttatt
     3181 acaaggcaat atctgaatgt ttcaattcac tgatattagt cagggtaatt acattaagaa
     3241 ttagttgaat accgttcttc tatttccatg tttattttat caatcttatc atcgtcgagt
     3301 tcatattttg aaataataaa cattgccagc ataagtaaaa ttgccggaat aacagctacc
     3361 agccataaaa ttccctgttg aacaaatggt gtctgcgaaa cggtgttttt ttcatcgaaa
     3421 ttaacaaaag ccagcacaaa accgggaaca acgccaccta atgccatgcc cgctttgaaa
     3481 aaaattccgg ttaaagcgtt gacgattcct gaaattcgtt taccggtagt atgttcgccg
     3541 tatgaaataa cttcgggaag caaagcccac atataacctg ttgcaacaat cacgcccgtg
     3601 gatttgacaa actgtgccac cagcaccagc caaaggtgtt gtctaagtcc gggaagtaca
     3661 gaaatgatat aaagcattgc cattccgaat atcgctaccg acaggaagac ataaaacatt
     3721 tgttttttac cgattgctct ttttatggca ggaatcatgg gcataaaaat aaatgcgggg
     3781 atagaaccca atgcggcgaa ataaggaagc caatcgggtg cctgaacatt ataaatcata
     3841 taataagatc cggcagaatt acctatagcc atcatagcaa aagcagttat aaagaaaaat
     3901 gccagtacac gcaacggacg attgtatttg aattcgttcc ataaatcaga aacttttaca
     3961 ttgtcagttt cctttacatc cattaccaca cgctcttttg tctgagtata acaaaaaata
     4021 agcagcgcca gtcctgccaa actatagata gtcatagtga taaaccaggc attgcccgat
     4081 gccggtgaat ttattttacc atcgggcgaa agagttttca caacgatagg aataccatac
     4141 gcaaccgcca atcctccgag atttgccaaa aacatacgaa cggaagtgag ttttgtaatt
     4201 tcgtccgtat ctcgggtaag cgaggcgttt aacgcaccat agggaacatt tatcagggtg
     4261 tataacatgg acaaacccac gtaagtgata taagcataca acaaggaacc tgaaaatcca
     4321 ttccaaaaac aaagaattgc aaaacctgta agcggaattc caccgagaag taaataggta
     4381 cggtatttac ccaattttgg attgtgttta tctacataag cccccaccag cggatcccac
     4441 actacatcaa ccaaacgaac gataagaaac attactgctg cagtggctgc cggaagtccg
     4501 aatacattgg tgtaaaaaat aagcagatac tgtgcaactg tttgataaat aagattttgt
     4561 gccaggtcgc ctgaaccaaa accgatacgc tgaatggcgg taagtttgta gaatcctctc
     4621 gattcgttag tttttgcttt tgaaattgat tccattttta tgtgtattat tatattttaa
     4681 tgcgattttt aatatgtaaa aatatggttt aaaaaaattc ctgtataata tcggtatata
     4741 cttacgttta ttttaaataa aaaaacgctg aaaatcgtta ttaccttttc cccggtgctc
     4801 ccaggtaact tttcttcaat ccgcccaaat caatcatgat cttttgaagc acaatgccgg
     4861 gttccaggac tctgaaacgc aagctgtgtt ttccgggttt atcaatttta tgacgggtaa
     4921 tggtttcgtt gatgcttttt tcctgccatt tttccattag cttaacatca tagttctgat
     4981 tgatatttac aatctgctca gttccaccat caaaagaaac cgcatagcgc aaacccttat
     5041 tatcattaaa attcagtgtt ggcgagacta aaatgtgaac aggaaattct cccgcgattt
     5101 caaaattgac atcgtattcc agatatacgg catctgttga ctttggatat acattttgtg
     5161 gaaaagtagt cacacccgac aatgttttac ccagatcggg aataatttcc cagtgaattc
     5221 ctcctgtttc gtttttacgg ctgtaatgtt ccgcttcaag tgatatgtaa cggttgtcga
     5281 atgtgcattt
//
LOCUS       DAOPZK010000130         5266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_19791_length_5266_cov_24.278066, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000130 DAOPZK010000000
VERSION     DAOPZK010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19791_length_5266_cov_24.278066"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PKH68_07595"
     CDS             <1..579
                     /locus_tag="PKH68_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNX12839.1"
                     /translation="TQILMDLANKHNGLVVGTGDLSESALGWSTYNGDHMSMYGVNSG
                     VPKTLVRYLVGWAAHQLDEQSEKILTDILDTPVSPELLPFDENGEIAQKTEDIVGPYE
                     LHDFFLYYFVRFGFTPEKIHFLAVTAFEEKYTGEVIEKWLKVFLKRFYQQQFKRSCIP
                     DGPKVGSINLSPRGDWRMPSDAVAPYPPKGEF"
     gene            717..1766
                     /locus_tag="PKH68_07600"
     CDS             717..1766
                     /locus_tag="PKH68_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNX12840.1"
                     /translation="MFLNNTPENWLISLAIIVGAVILNKLLSLFNRKVILKITRKTAN
                     RFDNILFTTLETPLLLGIMLVAIWVALDRLSLGKDTENIISKSYRILAVLNITWFVAR
                     LLVALAEEYLSSSNPKNAQRKVHLDIHMIALVKKTVLFIIWTLGGVMALSNVGVNIGA
                     LLGTLGIGGVAVALAAQDTVKNVFGGFTILMDGTFRIGDRIKIGEFDGFVENIGIRST
                     RIRTMEKRVVIIPNYKIVEGPVQNISEEPMRQVITRLGLVYSTSPKQMQEAIDILKSL
                     PGQITGVDTDDVAAVFSEYGESALVLKFIYHINSEGNVLTTPSEMNLKILHTFNAAGL
                     EFAFPTQTVYIQKNS"
     gene            1935..4292
                     /locus_tag="PKH68_07605"
     CDS             1935..4292
                     /locus_tag="PKH68_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNX12841.1"
                     /translation="MQKKDKSGWFFAVILVLFGVVTYLLALKGDSLDAALNSGKQVFT
                     AGSSDNYDLFKKLTLQNLLEPSGMILLQIIAILIVSRIFSSLFKRIGQPTVIGEILAG
                     IILGPSLLGYISPDTYHFLFSPKYLDTLYIVSQIGLILFMFTIGMDLSLSELKVKLKQ
                     TFIISQTSIIIPFLMGMILAYFIYYQFAPANTRFIAFALFIGISMSITAFPVLARIVQ
                     EKGLSKTHLGIVSIGSAAINDVTAWILLVAVIAIANTGSFTSSVFTLIFTLIYILAML
                     LAIRPFLKRIGNIYQHEELMNKNIFGFFILILIISAYTTQLLGIHALFGAFMAGLIMP
                     PLPRFRKLMMDKIEDLSVSLLLPLFFVYTGLKTEIGLLNTPDLWLLCLIFILVAVVGK
                     FGGGAITARITGESLKDSLSLGILMNTRGLMELIVLNIGLEMGILPPVIFTILVIMAL
                     VTTFMTTPFLSLIEFIFPEKEKKENLIRQQAEGVFKAMIALGNPENGKNLLKVAKTVL
                     DGAKNSLAVNVLHMTAGTDTNPIYGEQYSVESFIDVQAASKALNVPITTEYKVADNIE
                     AEIVKKINTEGYDFLLVGAGVSMNEDELMKENHWLERIPWLNRLTTQRAILYPGTLIR
                     DKTRYFIENSNCSVGIFVNRDFKGISSILIVLGDETDYFLLRYARRILRDNADVTVQI
                     IDINKLDGRTEAVSEGIQMLTGLFGSRVKLSKITRLHAGYLEKFSFLMISYVTWEALI
                     EAETQTLKDIPSTLIISKKSSRFSKSRKKRDVSVIPEPDDDLIEV"
     gene            4372..5211
                     /locus_tag="PKH68_07610"
     CDS             4372..5211
                     /locus_tag="PKH68_07610"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNX12842.1"
                     /translation="MPEQHQIDFERIAAAIEYMYINFRAQPTLDEVAAQVNMSPFHFQ
                     RMFQEWAGVSPKKFSQYLNVSNARNILKNSQLNLFDAAAETGLSGTGRLYDLFVKIEG
                     MTPGEYKNGGKSLSIKYVVSPGVFGQLLTASTGKGICYLAFSEDKEQMLNELKGLFPN
                     ATFTEQSDEKTEQVIRFFGRDWKHPNQIKLHLKGTPFQLKVWETLLRIPEGQLTTYGD
                     IAAQIQNPKATRAVGSAVGDNPVSFLIPCHRVIRSSGVLGEYHWGPARKKAMIGWEAV
                     RRK"
BASE COUNT         1498 a         1167 c         1200 g         1401 t
ORIGIN      
        1 acccaaattc tgatggattt ggccaacaaa cacaacggac tggtggtggg aaccggcgat
       61 ttgtcggagt cggcactcgg ctggtcaacc tacaacggcg accacatgtc catgtacggt
      121 gtgaacagcg gagtacccaa aacactggta cgctacctcg taggctgggc agctcatcag
      181 ctcgacgaac aatccgaaaa aattctgacc gacattctgg atactccggt aagtccggaa
      241 ttgctgccat ttgatgaaaa tggtgaaata gcacaaaaaa ccgaggacat tgtcggacct
      301 tacgaactgc acgatttctt cctgtactat ttcgtgcgtt ttggttttac acccgaaaaa
      361 attcattttt tggccgtaac ggcattcgaa gagaaatata ccggtgaagt gatagaaaaa
      421 tggctgaaag tattcctgaa acgcttctac cagcagcagt tcaagcgttc ctgcatcccc
      481 gacggaccca aggtgggaag catcaacctc tcgccgcgcg gcgactggcg catgccgagc
      541 gatgcagtag ccccctaccc cccaaagggg gaattctgaa cattttttca aaattgaaag
      601 aaaaaagaaa tatagtaata gtacgctcta aaattagtat aaactttaat caacactcca
      661 aaattccccc gcaatttatc ccaatatatc gggagggagt aaggtgacat ctaattatgt
      721 ttttaaataa cacacccgaa aactggctta tttctctggc aataattgtc ggtgccgtga
      781 ttctgaataa attactttcg ttattcaacc gcaaggtaat tctcaaaata acccggaaga
      841 cggctaaccg cttcgataat atcctgttca ccacactgga aacacccttg ttgctgggta
      901 tcatgctggt agccatctgg gtggctctgg acagactcag tctgggcaag gacaccgaaa
      961 acatcatttc caaatcgtac aggatactgg cggtactgaa tattacctgg tttgttgcca
     1021 ggttgttggt tgcattggca gaagagtatc tcagctcctc caatccgaag aatgctcaac
     1081 ggaaagtaca tcttgacatc cacatgattg ctttggtaaa aaaaaccgta ctttttatta
     1141 tctggacttt gggtggtgta atggcactga gcaatgtggg ggtaaatatc ggagcattgc
     1201 tgggcacatt gggcattggc ggtgtggctg tggcactggc tgcacaggat acggtgaaaa
     1261 atgtcttcgg cggtttcacc atactcatgg atgggacttt tcggattggc gaccggatta
     1321 aaatcggtga atttgacgga tttgtggaga atatcggtat cagaagcacc cgcatccgca
     1381 ccatggaaaa acgggtggtg atcattccca attacaaaat agtggaaggt ccggttcaaa
     1441 acatctccga agagcctatg cgacaggtta tcaccaggtt gggactggtt tacagcacct
     1501 ccccaaagca gatgcaggag gccattgaca tacttaaatc acttccgggt cagataaccg
     1561 gcgtcgatac tgatgatgta gctgctgttt tttcggaata cggcgaatcg gcactggtac
     1621 tgaaattcat ctatcatatc aattcggagg gtaacgtgct gaccacgcct tctgaaatga
     1681 acctgaaaat actccataca tttaacgcgg cgggacttga atttgctttt cccactcaaa
     1741 cggtttatat acagaaaaac agttaattca accttaattt tcttatcact tcaaagcgga
     1801 ttattgccaa acctaaaaaa atcttttccg ataaaaaaga agtaatctat ttttgactat
     1861 ctttgtgatg aaaaatccat tacaggaaaa aaatacaggt tttgacccaa catcaaaacc
     1921 ttctttgaaa caagatgcag aaaaaagaca aatccggatg gttctttgct gtaatattag
     1981 ttttattcgg cgttgtaact tatctccttg ccctcaaggg agatagcctg gatgcggctc
     2041 tgaattccgg aaaacaagta ttcactgcag gaagttccga taattatgat ttattcaaaa
     2101 aactgacttt acaaaattta ctcgaaccgt cggggatgat tttgttgcag attattgcca
     2161 ttctcatcgt ttcacgtatc ttcagttccc tgttcaagag aattggtcag cccaccgtta
     2221 tcggcgaaat attagccggt attattttag gaccttcgct gctcggatat atttcccccg
     2281 acacgtatca cttcctcttt tcgcccaaat acctcgacac actgtatatt gtcagtcaga
     2341 ttggattgat tctgttcatg tttactattg gtatggattt gagcctgagc gaattgaaag
     2401 tgaaactcaa acaaacattc ataatcagtc agaccagcat tatcatcccc tttctcatgg
     2461 gcatgatatt ggcctatttc atctattatc agtttgcacc ggccaatacc cgcttcatag
     2521 catttgccct gtttatcggc atttccatga gtataacagc tttcccggtg ttggcgcgga
     2581 ttgtgcagga gaaagggttg tcaaaaacgc acctgggcat cgtaagtatc ggcagtgccg
     2641 ccatcaatga tgtcacggcc tggattttgc tggtggcagt aatcgccatt gctaataccg
     2701 gtagctttac cagttcggtt tttacgctca tttttacgct gatatatata ctggcaatgc
     2761 ttttagctat acgtcctttc ctcaaacgta tcggtaatat ctatcagcat gaggaactga
     2821 tgaacaaaaa tatcttcgga ttttttattc ttatcctgat aatatcggcc tataccaccc
     2881 agttgctcgg tattcatgca ttgttcggcg ctttcatggc cggtctgatt atgccgccgc
     2941 tgcccaggtt ccggaaactg atgatggata aaatcgaaga tctgtccgtg tcactgttgt
     3001 tgccgctatt cttcgtttat accggtctga aaactgaaat cggactgctc aacacacccg
     3061 atctatggct gctgtgtctg atatttatcc tggtggctgt agttggaaaa tttggcggag
     3121 gagctatcac cgcgcgcatt accggcgaat cgcttaaaga cagtctctca ctgggtatcc
     3181 tgatgaacac ccgtggtctg atggaattaa ttgtgctgaa tatcgggctg gaaatgggta
     3241 ttttaccacc ggtaatattt actattctgg tgattatggc attggtaacc accttcatga
     3301 ctactccgtt tctttccttg attgaattta ttttccccga aaaagaaaag aaggaaaatc
     3361 tgattcgcca gcaggccgaa ggagttttca aagcaatgat tgcactcgga aatcccgaaa
     3421 acggaaaaaa tttgctgaaa gtggcaaaaa cagtattgga tggcgccaaa aattcattag
     3481 ccgtgaatgt gctccacatg acagccggca ccgataccaa cccgatttat ggagaacaat
     3541 actcggtcga aagttttatc gatgttcagg ctgcctccaa agccctcaac gtacccataa
     3601 ccaccgaata caaagtggcc gacaatattg aagcggaaat tgtaaagaaa ataaataccg
     3661 aaggttatga ttttctgctg gtaggtgcgg gtgtttcgat gaacgaggac gaactgatga
     3721 aggaaaatca ctggctggag cgaattccgt ggctcaacag actgaccacc caacgggcaa
     3781 tcctgtatcc cggtacactt atcagggata aaacccggta ttttatcgaa aacagcaatt
     3841 gcagcgtcgg tatttttgtc aaccgcgatt ttaagggcat cagttccatt ttaatagttc
     3901 ttggagatga aaccgattat tttctgctcc ggtatgcccg ccgaattctg cgtgataatg
     3961 cagatgtgac cgttcagata atcgatatca acaaactgga cgggcgcacg gaagctgttt
     4021 ctgagggaat tcagatgcta accggtttgt tcgggtcacg ggtcaaattg agtaaaatta
     4081 cccgtctgca tgccggttat ctcgaaaaat tcagtttcct gatgatcagc tatgtgacct
     4141 gggaagccct gatagaggca gaaacacaaa cactcaaaga cataccttcc acactgatta
     4201 tcagcaaaaa aagcagccgg ttcagcaaaa gcagaaaaaa acgtgatgta tctgtaatcc
     4261 ctgagccgga cgatgatctc attgaagtct gaagcaagcc ggatttagca agaatcgggt
     4321 tgctccctct gactgattca tttatttttg ccgaaaaaca aatcataaaa catgccggaa
     4381 caacaccaaa ttgattttga gcggatagct gctgccatcg aatacatgta catcaacttc
     4441 cgggcgcaac ccaccctgga cgaagtggct gcacaggtca acatgagtcc gtttcatttt
     4501 cagcgaatgt ttcaggaatg ggcaggggta agtccaaaaa aattcagtca gtacctgaat
     4561 gtgagcaatg cccgcaatat actgaaaaac agccagctga acctgttcga tgcggctgcc
     4621 gaaacgggac tttcgggcac gggacgtttg tacgatttat ttgtgaaaat tgaagggatg
     4681 acgccgggag agtataaaaa cggagggaaa agcctgagta taaaatacgt tgtatcacca
     4741 ggtgttttcg gtcaactgct gaccgcttcc acaggcaaag gaatttgcta tctggcattt
     4801 tctgaggata aagaacaaat gttgaacgag ctgaagggat tatttcccaa tgccacattt
     4861 acggaacaat cagacgagaa aacagaacag gttatccggt ttttcgggcg ggactggaag
     4921 catcccaacc aaatcaaatt gcatctgaaa ggcacccctt ttcagcttaa agtgtgggaa
     4981 acgctcctca gaattcctga aggacaactc accacctacg gcgacattgc ggcacaaatt
     5041 cagaatccaa aagccacccg cgccgttggc tcagccgtgg gcgacaatcc cgtatctttt
     5101 ttgataccct gccaccgggt tatacgttca tcgggtgtgc tgggtgaata ccactgggga
     5161 ccagcccgca agaaagccat gatcggctgg gaagcggtaa ggagaaagta gtgtggaatg
     5221 tgtagtgtgt agtgtgtagt gtggaatgtg gaatgtgtag tgttta
//
LOCUS       DAOPZK010000131         5252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_19871_length_5252_cov_19.590533, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000131 DAOPZK010000000
VERSION     DAOPZK010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19871_length_5252_cov_19.590533"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..545)
                     /locus_tag="PKH68_07615"
     CDS             complement(69..545)
                     /locus_tag="PKH68_07615"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNX12843.1"
                     /translation="MSRVVIIMGSKADLDWAEQISRVLKGFEIEVISRIASAHKVPLK
                     CYNLIKDYEKENVVFITIAGMSNALSGFTDAQTHCPVIACPPYSDKFGGADLYSSIRM
                     PSGVAPLTVLSPENAALAAAKILGLSNPGIQKRIIEFQDKKRKELEEADNNLVNEK"
     gene            complement(514..2469)
                     /locus_tag="PKH68_07620"
     CDS             complement(514..2469)
                     /locus_tag="PKH68_07620"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HNX12844.1"
                     /translation="MSNLLTVLNSKNIIVPDNISTLLNECKSYTVFNTSEELAIAATN
                     GIENMEFEVSYDVPGKGNYVEAIVQRVKNGISANYTEAYMRRRDPDTMVIADNNPSDK
                     KRFIDKYGYEFSGLQTETFDWLKKQDLAVFFYFAGRENIGSLGIAIAPANAAFFALGL
                     SMLQEMVAVDQLKVGAKIKSVIFVAPIFRHTHFDGKQVVVHNRTESVHELYSYNLYPG
                     PSAKKGLYGVMLNHAEKEDWIIAHCSAVQSISPYDNLTTFMHEGASGGGKSEMHQHIL
                     RESDGRVLLGRNLVSAEERFITIPRFCSFNAVADDMAFCHPSVQKKDGKLRIIDAENA
                     WFVRVDSVKQYGDDPTLEKNTINPISPLMFLNIETTPGSTALIWDHKLDEPGKPCPNP
                     RVIIPRNAVPNVVNKPVSVDIRSFGIRTPVCSKENPSYGIIGLFHVLPPALAWLWRLV
                     APRGHNNPSIVSSGGMESEGVGSYWPFATGKRVPQANMLLKQIIDTPRTTFTLVPNQN
                     IGVWQVGFKPQLLMREYLTRRGAAQLKTDQYQAARCPLLGYELNYLTLEGSKIPSRFL
                     KVYNQPEVGFEGYDAGAEMLRDFFKTELQKYLKEDLLHTGKRIIDACLSNASIEEYNE
                     IIPMSYQYEYSFNKIEDYEQSGDNNGF"
     gene            complement(2466..3011)
                     /locus_tag="PKH68_07625"
     CDS             complement(2466..3011)
                     /locus_tag="PKH68_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNX12845.1"
                     /translation="MTKHPAIFLDRDGTLIDDAGYIQHSSEVSFYPFTFEALSLLQEY
                     FLLFIITNQSGIAKGITTESEVSAVNNYIVNALKEQGIDIYEVFCCPHNTEDYCECKK
                     PKTYFIDKAQQLYNIDLSRSFIIGDHPSDIQCGLNAGISPVYLLTGHGQKHLNEIDAD
                     TVICSDILEASKYIILKNNQI"
     gene            complement(3088..3639)
                     /locus_tag="PKH68_07630"
     CDS             complement(3088..3639)
                     /locus_tag="PKH68_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNX12846.1"
                     /translation="MNRIGERIKKKREQLNLQLNELAEKIGISPSALSQIEKAKSFPS
                     IITLKTIAEKLHTTVGELIGENDSLANNPVVHKSDIRFIDQNKSGTVFYLLSQHDVNK
                     QMETYLIRFSKASAIDGFITSTFGQVFAYVLSGEVRFDLDGKSFILKAGDNIYFNAKS
                     QFNAINFYDGISEIIWVQSPPHF"
     gene            complement(3902..5065)
                     /locus_tag="PKH68_07635"
     CDS             complement(3902..5065)
                     /locus_tag="PKH68_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNX12847.1"
                     /translation="MKTTLKIDITVKDICEGFVYNELEGKGLFGLSGKLTIQPEYQRN
                     YIYADGKKDVAVIESILKGYPLGLIYFVKVNEEKFEVLDGQQRITSFGRFVTNKFAIR
                     DENGMEQYFSSIAADKRAKILEAKLLIYECEGIETEIKQWFRTINIAGVPLTNQELLN
                     AVYSGPFVTLGKEEFSNSQNSNIQKWSAYISGSVNRQDFLERALEWVSKGNVENYMSR
                     HRFDANITELKNYFNSVIDWVSTVFTDVESEMRGLEWGRLYEEYHSKAYNPAKVSEEV
                     QKLYGDLYVKNRKGIFEYILGGSVDTKLLEVRVFDEVIKRSVYSTQTAEAETKGESNC
                     PLCAIGHDANKNKIWKLAEMDADHVSAWSKGGATDASNCQLLCKTHNRAKGNR"
BASE COUNT         1457 a         1124 c          998 g         1673 t
ORIGIN      
        1 atactgtttc agtcgggaaa gccaccaaac caccattttt aatgatttga gcagcttcgt
       61 aaatgttgtt atttttcatt taccaaatta ttatcggctt cctctaactc ttttctcttt
      121 ttgtcctgaa attcaataat gcgtttttga ataccggggt tcgataaacc cagtatctta
      181 gccgccgcta aggctgcatt ttcggggctt aaaacggtta aaggagctac tcccgaaggc
      241 atacgaatac tcgaatataa atcggcacca ccaaatttat cgctgtatgg cggacaggca
      301 ataaccggac aatgagtttg agcatcggta aagccactca gagcgttgct catgccggca
      361 atggtaataa atacaacgtt ttccttttca taatccttaa tcaggttgta gcattttaag
      421 ggcaccttat gagccgaagc tattctggag attacctcta tttcaaaacc ttttaaaacc
      481 ctgcttattt gttccgccca atcaagatcg gctttagaac ccattattat caccactctg
      541 ctcatagtct tctattttat tgaatgaata ttcgtattgg tagctcatcg gtatgatttc
      601 gttgtactct tcaatgcttg cgtttgacaa acaggcgtca attatccgtt tgcctgtatg
      661 caacaagtct tccttcaggt atttctgcaa ttcggttttg aaaaaatcac gaagcatttc
      721 ggctccggca tcgtaacctt cgaaacccac ttcaggctga ttgtacactt tcaaaaacct
      781 tgaaggaatt tttgaacctt caagcgtaag gtaattcagt tcgtaaccta acagcgggca
      841 tcgggctgcc tgatattgat ccgttttaag ttgtgcagca cccctgcgtg taaggtactc
      901 tcgcatcaga agctgcggtt taaaacctac ctgccatact cctatatttt gatttggaac
      961 cagtgtgaaa gtggtacgtg gtgtatcaat tatttgttta agcagcatat tggcttgtgg
     1021 cacacgtttt ccggtggcaa aaggccaata agaaccaaca ccttcacttt ccataccgcc
     1081 cgaacttacg atgcttgggt tgttgtgacc gcgtggtgca accaatcgcc acagccaggc
     1141 cagtgccggt ggcaacacat gaaacaaccc gataattccg tacgaagggt tttcttttga
     1201 gcacaccggg gtgcgtatcc cgaaactccg gatatcgaca gaaacaggct tgtttacaac
     1261 attcggaacc gcatttctgg gtattatcac ccttggatta gggcagggtt taccgggttc
     1321 atccagttta tgatcccaga ttaaagcagt tgacccggga gtggtttcaa tatttaaaaa
     1381 cattaacgga gaaattggat taatcgtgtt cttttccaat gtggggtcat caccgtattg
     1441 ttttacactg tcaacacgca caaaccaggc attttcggca tcaataatac ggagttttcc
     1501 gtcttttttc tgaacagaag ggtggcaaaa agccatgtca tcggccacag cattgaacga
     1561 acaaaagcgg ggaatagtaa taaatcgttc ttcagcagac accaaattgc gtcctaaaag
     1621 tacccgaccg tcagattcgc gcagaatatg ctggtgcatt tcgctttttc cgcctccact
     1681 ggcgccttcg tgcatgaatg tggttaaatt gtcataggga ctgattgatt gcactgccga
     1741 acaatgggca attatccagt cttctttttc ggcatggttc aacataacgc cgtaaagacc
     1801 ttttttagca cttggtccgg gatacaggtt ataggaataa agctcgtgaa cagattcagt
     1861 tcggttatga acaacaacct gtttcccgtc gaaatgggta tgtctgaata tgggtgctac
     1921 aaatattacc gattttattt tggcacctac ctttaattga tcaactgcaa ccatttcctg
     1981 caacatcgac aaacccaagg caaagaatgc ggcattggcg ggagcaatgg cgatacctaa
     2041 cgagccgata ttttcccttc ctgcaaaata gaaaaaaacg gccaggtctt gttttttcag
     2101 ccaatcgaaa gtttctgttt gcagaccgga gaattcataa ccgtatttat caatgaagcg
     2161 ctttttatcc gatgggttat tatccgcaat aaccatggtg tcggggtctc tgcggcgcat
     2221 gtatgcttcg gtgtaatttg ccgaaattcc atttttaaca cgctgtacta ttgcttctac
     2281 gtaattacct ttgcctggta cgtcgtatga tacttcaaat tccatgtttt ctattccgtt
     2341 ggtggctgca atggccaatt cttccgaagt gttgaatact gtatagcttt tgcattcatt
     2401 caacaacgtg gagatattat ccggaacgat aatattctta ctatttaata ctgtcaataa
     2461 attactcata tctgattgtt ttttaggatt atatactttg atgcttctaa gatatctgaa
     2521 caaattaccg tgtccgcgtc tatttcgttt agatgttttt gaccatgtcc ggttaataaa
     2581 taaactggag aaattccggc attcagtccg cactgaatat ccgaaggatg gtcgccaata
     2641 ataaatgaac gactcaaatc aatattgtac aattgctgag ccttgtcaat aaaatacgtt
     2701 ttgggtttct tacattcaca atagtcttcg gtgttatgcg ggcaacaaaa aacctcataa
     2761 atatctatac cctgctcttt taatgcgtta actatataat tattaacggc tgaaacttca
     2821 ctttctgttg taattccttt ggcaatacct gattgattgg taataataaa taacagaaaa
     2881 tattcctgta acagtgaaag agcttcaaat gtaaaaggat aaaagcttac ttcagatgaa
     2941 tgttgaatat aacctgcatc atcaatcaac gttccgtctc tgtctaaaaa aatcgcagga
     3001 tgctttgtca ttgctctgaa tatttcaacg acaaagataa tgctttttac ttaacaaata
     3061 aagtaatact taatttaatt tgaaaattta aaaatgagga ggagattgca cccagattat
     3121 ttcactgata ccatcgtaga agttgatggc attgaactgt gatttggcat taaagtatat
     3181 attgtcaccg gctttgagaa taaaactttt tccgtccaaa tcaaaacgaa cttcgcccga
     3241 caggacataa gcaaatacct gaccaaaagt gctcgtaata aatccatcga tagccgatgc
     3301 ttttgaaaac cggataaggt aggtctccat ttgcttattc acatcgtgtt gtgatagcag
     3361 ataaaacact gttcccgact tattctggtc aataaacctg atgtctgatt tatgtaccac
     3421 cggattgttt gccagcgaat cattctctcc aatcaactca ccgaccgttg tatgcagttt
     3481 ctcagcaatg gttttcagtg ttatgatgga aggaaaagat ttagcttttt caatttgcga
     3541 caaggcactg gggctaatgc caatcttttc agccagttcg ttaagttgta aattaagttg
     3601 ctcgcgcttc ttctttattc tttctccaat tctattcata attatcaatg gtttgaactg
     3661 caaagatata atttaattaa tgaagtagca cttaattttt gaaatttcta taacagttga
     3721 gtttattttg tttgtttgtg gtatttatat tattgattta gaactaatta cagaatacat
     3781 ttttaaccgg gagattaagc gacaggtaaa ccgtttgaat tttatataaa atcacctcaa
     3841 ttattttacc aaaacactgc ctgtagttat cacaccacca ttgacaactt taacggtata
     3901 attacctgtt tccctttgcc cgattgtggg tcttacacaa tagctgacaa ttacttgcat
     3961 cagtagcacc acccttgctc caggctgaaa cgtggtcggc atccatttcg gctaacttcc
     4021 agattttgtt cttgttggca tcatgaccga tggcgcacag cggacagttt gattcacctt
     4081 tagtttcagc ttcagctgtc tgagttgagt aaacggatct cttaataact tcatcaaaca
     4141 cccgaacttc aagcaatttc gtatcgactg aaccgccaag gatatactcg aatatacctt
     4201 ttcggttttt tacatacaag tcaccgtaga gtttctgcac ttcttccgac acctttgccg
     4261 gattataggc tttgctgtga tattcttcgt acaatcgtcc ccattccagt ccgcgcattt
     4321 cgctttcaac gtctgtaaat acagttgaaa cccagtcaat cacgctgttg aaatagtttt
     4381 ttaattcggt aatattcgca tcgaaacgat gacggctcat atagttttca acgttacctt
     4441 tgctaaccca ttccagtgca cgttccaaaa aatcctgtcg gttgacactg ccggatatat
     4501 aagcgctcca tttttgaatg ttggagttct ggctgttgct aaactcttcc ttaccgagtg
     4561 taacaaaagg tcctgaataa acggcattca gcaattcctg attagtgaga ggtacaccgg
     4621 caatattaat cgtcctaaac cactgtttta tctcggtctc gataccttca cattcgtaaa
     4681 tgagcagttt cgcttcgagt atcttagccc gtttatctgc cgcaatgctg ctgaaatatt
     4741 gctccatacc gttttcatcc ctgatggcaa acttgttggt gacaaatcgg ccgaaactgg
     4801 taatacgctg ctgaccgtcc aacacttcga acttttcttc attcactttt acaaaataaa
     4861 tcaaccccag cggataacct ttgagaatgg attcgataac ggccacatct tttttgccgt
     4921 cggcatagat atagttgcgc tggtactcgg gctgaatggt cagtttgccc gacaaaccga
     4981 acaaaccttt accttccagt tcgttgtaaa caaatccttc gcagatgtct ttgactgtga
     5041 tgtcaatttt gagagtggtt ttcatgctat aataatttat ttatctgccc cctaaccccc
     5101 taaaggggga atttggagca ttaaattaga tttgtacaaa tagttaagtg attattatca
     5161 ttccagcgtg tgtccaatat tcttagttgg acatgggtgt ttaatgtgct attgaattct
     5221 ctgtgtatta tttattggaa cgctgatgac gc
//
LOCUS       DAOPZK010000132         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_19879_length_5251_cov_16.404349, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000132 DAOPZK010000000
VERSION     DAOPZK010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19879_length_5251_cov_16.404349"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="PKH68_07640"
     CDS             complement(<1..1355)
                     /locus_tag="PKH68_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNX12848.1"
                     /translation="MMKNKVISAVVFLMVLVLSNAQNPIIQTMYTADPAPMVYNGKLY
                     LYTSHDEDNSSWFVMNNWKLYSTTDMVNWTDHGVVLSYSDFSWAKGDAWAAQCIERDG
                     KFYMYVPVTSRLNNRPAIGVAVADSPYGPFYDPLGKPLVQSGHGDIDPTVFIDDDGQA
                     YLYWGNPNCYYVELNEDMISFKNDIVRVPMTEESFGKREGNVKDRPTLYEEAPWLYKR
                     KDMYYLLWGGGPIPEHLGYSTGKSPKGPWKNQGMLMPTEGRSFTNHPGIIDYKGKTYL
                     FYHNGALPGGHGFNRSVCVEEVKFNKDGTIQPMKMTEGIQSGLETLNPYRKNEAETIA
                     FSEGVKASQNKQVGVFVNAMQNNAYTKVKGVDFGTQGASRFSARVGTTINGGVTMEIR
                     LGSKDEKLIGTIRVPITGGDDRWEVVSTDIEKVTGTQDLYFVFKSDKPGRIMYFDYWM
                     FS"
     gene            complement(1368..>2672)
                     /locus_tag="PKH68_07645"
     CDS             complement(1368..>2672)
                     /locus_tag="PKH68_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12849.1"
                     /translation="NEDPKNFNFFEASSIRKVGNKYITVFSGYSGPEYGLGSTNSSLR
                     YAVADSPLGPWKSGGVLVDSRGPVLNQDGSGLQTTSGGHNTHGSIENINGQWYVFYHK
                     PPRNFGFARQAVVAPITIEWDEKPVAEGGSAVIRAYDPYAKNKIWTAKDNQGREYTGA
                     EITSEGFHIYGLDPYQYYSAGYASYLSDVSIQQDSWDIWDNHMPITNVKNGHIIGYKY
                     FGFGGLKKATKGLNAFKGTKRGNKTEFNLFLTPKTSKSFKVNVWLDGPWDNDTWKGKK
                     IGEIIVPANSKQNVTKFTVNVSKSVDKLKKKHAIFLVAEGDGSEELFDLSGLGFSSKK
                     NKIERPIVPTVSISVNGQKIDLPKTPVRSTNENGIVGYDIYEATYKLPAETTIVPKVT
                     ASADNNDVKIKVTQADSATGTANVEFDYNGVVKTYRVVFTNN"
     assembly_gap    2673..2772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2773..3269)
                     /locus_tag="PKH68_07650"
     CDS             complement(<2773..3269)
                     /locus_tag="PKH68_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12850.1"
                     /translation="MSQTKTAPRKNLSERFVHKSIGNPYLPLWEHLPDGEPRVFEDPD
                     NPGKYRAYIIGSHDLRVNSYCGPDIRVWSAPVEDLSSWRDKGPVFTYFIDNQWDVMYA
                     PDLVEVKRKDGTKEYYLYPHSRGPHREAMVAKGSRPDGPFKPINLTEDGRRTLPGSIL
                     GFDPA"
     gene            complement(3485..>5251)
                     /locus_tag="PKH68_07655"
     CDS             complement(3485..>5251)
                     /locus_tag="PKH68_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNX12851.1"
                     /translation="ENITLHGFAQTYSPFFNQNAWNNYSVSGCLTYNKRMIDRIMGTG
                     WKMNFVRMHMDPYWSDDPTQASVRYEGHERFSQERFKKYLDEVFVPMAEHAISKGLYV
                     VLRPPGVSPGKIAPGDDYNKFLELVWDIVSKHPKIKNNSDIMFELANEPINILGPNGT
                     YASSGQGHFDQMKLFCQSIVDKIRANADNIIWVPGLAYQSSFSGFANNPVKGTNIGYA
                     VHAYPGWYGSDTEEASPELGGSMGGGYESFQRGWNAQVKPVADIAPIMVTEMDWAPKK
                     YEASWGKSFTGVVGGSGFGANFKYIADITGNVSWLIFTECHRLAEFNDIAGTAGNYTF
                     LNDPEACVWPTFHWYKEYASGKEDSVTESELAKIEVVGVSGNLTMLTGGDRYLIVNAI
                     YNNGTSLPVTSKTVFTTSDQSVVSINENGRLIALKDGSATITATFTPSTGVSKQVSFT
                     VYSTTFPLTKELFNPSIFAQGTFDEETRTLVTGQWGFGGWWYNKGVDLSKYKYLIAEL
                     GNDNNSSVSFRLFDENNYWSQPATYDFGNSRKVVVELGNMYKKINGVQVKLNPAQIYI
                     IGFWSSGGSPIIIKGVYLSDSL"
BASE COUNT         1350 a         1220 c          964 g         1617 t
ORIGIN      
        1 gaaaacatcc agtaatcgaa atacatgatt cttcccggtt tatcggattt gaaaacaaaa
       61 tacaggtctt gcgttccggt aactttctca atatcggtac tcaccacttc ccaacggtcg
      121 tcaccgcccg taatcgggac tcttatcgtt ccaataagct tttcatcctt gcttccgagg
      181 cggatttcca ttgtcacacc tccgttgatg gttgtgccta cgcgagctga aaatcgggat
      241 gctccctgag ttccaaaatc aacgccttta acttttgtgt atgcgttatt ctgcatcgca
      301 ttcacaaata cgcccacttg cttattctga gaagccttta ctccttcgga aaaagcgatg
      361 gtttctgctt cgttttttct gtatggattc aaagtttcca gtccgctttg gattccttcg
      421 gtcattttca tcggctgtat ggttccatct ttgttgaatt tgacttcttc cacgcacacc
      481 gagcggttga aaccgtgccc gccgggaagt gctccgttgt ggtaaaaaag gtaggttttc
      541 cctttgtaat caatgattcc tggatgattg gtgaaacttc tgccttcagt aggcatcagc
      601 attccctgat ttttccaggg tcctttcgga cttttgccgg ttgaataacc caaatgttcg
      661 ggaatgggtc cgccacccca aagcaggtag tacatgtctt tccgcttgta gagccaaggt
      721 gcttcctcgt acaaagtcgg tcggtctttc acatttcctt ccctttttcc aaaagattct
      781 tcggtcattg gaacccgtac aatatcattt ttaaaagaaa tcatatcttc gttcaactcc
      841 acgtagtagc agttcggatt tccccagtag aggtacgctt gtccgtcatc gtcaatgaat
      901 acggtggggt caatatcacc gtgaccgctt tgcaccagcg gttttccgag cggatcgtag
      961 aaaggtccgt aaggactatc tgcaacagcc actccaatcg cagggcggtt atttaatcgg
     1021 gatgtaactg gcacgtacat gtaaaatttt ccgtctctct caatgcattg tgccgcccaa
     1081 gcatcaccct ttgcccatga aaaatcagag tatgaaagta ccacgccgtg atcagtccaa
     1141 tttaccatgt cggtagtaga ataaagtttc cagttattca tcacaaacca ggaggaattg
     1201 tcctcgtcgt ggctggtgta gagatacagt ttgccattgt acaccatcgg tgccggatcg
     1261 gcagtgtaca tggtttgaat aattgggttc tgagcattcg aaagcaccaa aaccatcaaa
     1321 aacacaacgg ctgatattac tttattcttc atcattttgc agctttttta attgtttgtg
     1381 aaaacaaccc tataggtctt tacaactccg ttgtaatcaa attctacgtt tgctgttcct
     1441 gttgcagaat ctgcctgcgt tacttttatt ttcacatcgt tattgtcagc tgatgccgtc
     1501 actttaggaa cgattgtcgt ttcagcaggg agtttatagg ttgcttcgta aatatcgtac
     1561 ccaacaattc cgttttcatt ggtagatcta acgggtgttt tcggcaaatc aatcttttgt
     1621 ccgttcacag aaatagaaac agtcggtaca ataggacgct caattttatt tttctttgag
     1681 ctgaaaccaa gtccgcttaa atcgaacagt tcttccgatc catcgccttc cgcaactaaa
     1741 aatatcgcgt gtttcttttt caatttatct actgatttag aaacatttac agtaaatttt
     1801 gttacgttct gtttcgaatt agcagggaca ataatttcac ctatcttttt ccccttccaa
     1861 gtatcgttgt cccaaggacc gtctaaccaa acatttactt tgaaactttt tgaagtttta
     1921 ggagtgagaa acaagttgaa ttctgtttta ttccctcttt tggttccttt gaaagcatta
     1981 agacctttgg tagctttttt aagtcctcca aatccaaaat atttataccc gataatatgg
     2041 ccgtttttca catttgttat cggcatgtga ttatcccaaa tatcccatga atcttgctga
     2101 atgctgacat cggaaaggta gcttgcatag cctgcagaat aatattggta cggatcaagc
     2161 ccgtagatat gaaagccttc cgacgtaatt tcagcacccg tgtattctct tccctggttg
     2221 tcttttgctg tccaaatttt attttttgca taaggatcat atgctctgat tactgcgcta
     2281 ccgccttctg ctactggttt ttcgtcccat tctatcgtaa tcggagccac cactgcctga
     2341 cgtgcaaaac cgaaattcct tggtggttta tgataaaaca cgtaccattg gccgttaata
     2401 ttttcgatgc tgccgtgcgt gttgtggcca ccgctggtgg tctgcaatcc cgagccgtcc
     2461 tgattcaaaa caggtccccg cgagtcaacc aaaacgccac cgcttttcca gggcccgagc
     2521 ggtgaatcgg caaccgcata acgcaacgaa gaattagtac ttcctaatcc atattcaggt
     2581 ccggaataac cgctgaaaac cgtgatgtat ttgtttccaa ctttccggat ggatgatgct
     2641 tcaaagaaat tgaaattttt cggatcttca ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnatggccgg gtcaaaaccg agaatactgc cgggaagcgt tcttcttcca
     2821 tcttcggtaa gatttatggg cttaaatggg ccgtcaggac ggcttccttt ggctaccatt
     2881 gcttctctgt gcggaccgcg gctgtgcgga taaaggtaat attctttggt gccgtctttt
     2941 cttttcactt ctactaaatc aggtgcgtac atcacatccc attggttatc aataaaatat
     3001 gtaaaaacgg gacctttatc gcgccaactt gacagatctt ctaccggtgc cgaccaaacg
     3061 cggatatcgg gtccgcaata gctatttaca cgcaaatcgt gcgaaccaat gatgtaagca
     3121 cggtattttc ccggattatc aggatcttca aatacgcgtg gttcaccgtc aggcaggtgc
     3181 tcccaaagcg gtaaataggg atttcctatt gatttatgga caaatctttc tgatagattt
     3241 tttcttggag ctgttttagt ctggctcatc aatccggtcg aggaaaataa gaacatcgcg
     3301 atgaaaaaaa tcgcgtatct ctctttctgt aaaaattcta atctcttcat ataaaataca
     3361 ttattaataa aaattcaaca catatatcat taacaacaag cgaaaataac cattgctttt
     3421 tgagcaaatt tcattgcttc taccatagtt aaaataatgg tgtaagcacc aacccggatt
     3481 tttattaaag actatccgac agataaacac cttttatgat tatcggactg ccacccgatg
     3541 accaaaagcc aataatatag atttgtgccg gattaagttt gacctgtact ccatttattt
     3601 ttttatacat gttcccaagt tccacgacta cctttcttga gttgccaaaa tcatacgtgg
     3661 ccggctgaga ccaataattg ttttcatcaa aaagtctgaa agagacactg ctattattat
     3721 cgttaccaag ttcagcaatg aggtatttat attttgacaa atctactcct ttgttatacc
     3781 accatcctcc aaagccccat tgtccggtaa ctaaggttcg cgtttcctcg tcgaacgtcc
     3841 cttgcgcaaa aatagatgga ttaaacaact ctttagtcag aggaaaggta gttgagtaaa
     3901 cagtaaagct aacctgttta ctaactcctg ttgaaggtgt aaatgtggcc gttatggttg
     3961 ctgatccatc ttttaatgct atcaaccttc cattctcatt tatacttaca acagattggt
     4021 ctgaagttgt gaacactgtt tttgatgtaa ccggcaaaga tgtgccgttg ttgtaaattg
     4081 catttacaat caaatacctg tctcctccgg ttaacattgt caagtttccg ctcacgccta
     4141 caacttctat ctttgctaat tcactttccg ttaccgaatc ctctttacct gatgcatatt
     4201 ctttatacca gtggaatgtt ggccacacac aagcttcagg atcattcagg aaagtgtaat
     4261 ttccagctgt tcctgcaata tcattaaact cagcaaggcg atgacattcg gtaaaaatca
     4321 accaactcac attccctgtt atgtctgcga tatatttaaa attagctcca aaaccagaac
     4381 ctcctaccac tccggtaaaa cttttgcccc atgatgcttc atattttttg ggtgcccaat
     4441 ccatttcagt gaccatgata ggagcaatat ctgctacggg tttcacctgt gcattccacc
     4501 cacgctgaaa actctcatat cctcctccca tcgaacctcc caactccgga cttgcctctt
     4561 cggtatcgct accgtaccat cccggatatg cgtgaacagc ataaccaata tttgttcctt
     4621 taaccggatt attagcaaat ccactgaaag aagactggta ggccagcccc ggtacccaaa
     4681 ttatattatc ggcatttgcc ctaatcttat caacaatgct ttgacagaat aatttcatct
     4741 gatcaaagtg cccctgtccg ctacttgcat acgtaccatt tggtcccaat atatttatag
     4801 gctcattagc cagttcaaac attatatcgg aattattttt gattttagga tgttttgaca
     4861 caatgtccca tactaactca agaaatttat tgtaatcatc tcccggcgca atttttcccg
     4921 gacttacgcc cgggggacgc aacaccacat ataacccttt gctgatggca tgttcagcca
     4981 ttggaacaaa cacttcgtcc aggtatttct tgaaccgctc ctgagaaaaa cgttcatgcc
     5041 cctcataacg aacagatgcc tgagttgggt cgtcgctcca gtatggatcc atgtgcattc
     5101 tcacaaaatt catcttccat ccggtgccca taatgcggtc aatcatccgt ttgttatatg
     5161 ttaaacaccc ggatacgctg taattattcc atgcattttg gttgaaaaac ggactgtatg
     5221 tttgcgcaaa tccatgcaat gtgatgtttt c
//
LOCUS       DAOPZK010000133         5244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_19921_length_5244_cov_24.026209, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000133 DAOPZK010000000
VERSION     DAOPZK010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19921_length_5244_cov_24.026209"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..512)
                     /gene="rplI"
                     /locus_tag="PKH68_07660"
     CDS             complement(66..512)
                     /gene="rplI"
                     /locus_tag="PKH68_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNX12852.1"
                     /translation="MEIILKEDVINLGYKGDIVKVKDGYGRNYLIPTKKAVIASESAK
                     KMLAEDNRQRAHKFEQIKNNAVELSEKIAALGTLTIGAKTSSTGKIFGAVGPIQIAEA
                     IEKAGLIVDRKIIALKDPIKEIGSYTASIKLHKEVSAEISFEVVSE"
     gene            complement(535..804)
                     /gene="rpsR"
                     /locus_tag="PKH68_07665"
     CDS             complement(535..804)
                     /gene="rpsR"
                     /locus_tag="PKH68_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNX12853.1"
                     /translation="MAAHSGEIRYLTPPTVDVKKKKYCRFKKSGIKYIDYKDAEFLKK
                     FLNEQGKILPRRITGTSLKYQRKVAQAVKRARHLSLLPYVTDMMK"
     gene            complement(808..1203)
                     /gene="rpsF"
                     /locus_tag="PKH68_07670"
     CDS             complement(808..1203)
                     /gene="rpsF"
                     /locus_tag="PKH68_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNX12854.1"
                     /translation="MLNHYEAVFILTPVLSDVQMKEAVDKFKAVITDNGGTITNEENW
                     GMRKLAYPIQKKSTGFYALLQFDVEPTVIATLETQFRRDERVLRFLTFRLDKYAFEYA
                     EKRKNVKSQPKEVVEEKAAKKELKEEKEN"
     gene            1504..3411
                     /locus_tag="PKH68_07675"
     CDS             1504..3411
                     /locus_tag="PKH68_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNX12855.1"
                     /translation="MKRYILIFSLILAGIHAFSQTDSIASPKQKSTKPVKHIKVWHIS
                     EQLAVADTIPMDTATINFPDQNPVETFSIANSYGGNLGLPLQSKLYFKRPQGSDFLFD
                     NAYKPYTLDVSSASFYDTTFPFTNLTYLTGGSAYRKEEQVKFLFTASPSGKANFGTTL
                     DYIHAVGEYNNQAAKRFSGGIFGRYNGKHYSANGLVAVNNHFNYENGGLSDLSYLTNP
                     DLNVKPKDIPVAITGYSVFRKNQFYYNHGYSIGFNREVKVSADSVRSEYVPVTRFGHT
                     IKLEEMKKRYYEPTLERKFYEFSDTTRKIANDTAAVRTLTNLVSISLEEEFNKWMKFG
                     LTGYVENEIQQFTYQPDTTLYRATKSNTRVGGVLSKNRGTNFRYSVLGDIYLIGYKLG
                     EFRLEGKAVGNFRLFNQQISLSANAFIRNEEPAYYLQHYYSTHFRWDNEFSKMYKTHV
                     SGSFSLPKLYTQLNVGVENITDYIYFNEKALPSQYQGNIQVLSADLKQDFHFGKFVLE
                     NNAVYQVSSVPDVLPLPGLTLFHNFYYYSKWFKDLYPQLGASVRYHSNYYAPAYMPAT
                     GQFYTQKEVLIGNYPILNVYANFHLKQARFFIEYNHLNKYFMSGAHFYMPNYPINPPV
                     MKLGLSWNFYN"
     gene            3429..4247
                     /locus_tag="PKH68_07680"
     CDS             3429..4247
                     /locus_tag="PKH68_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNX12856.1"
                     /translation="MKRCTKINTGVVLGLIALICIGVIVTHKVYTPTVKRDFSEIRKS
                     GRLRVVTDKSSLGFKLYDDKIEGFNYELAKAFANSMGLELEIIPENNLDSALNGIKEN
                     KYDLLAMNIPNTAETKKELALTIPILSGRQVLVQQEIIDTTNLKAIRSHYLLTNDSIC
                     IPVRSPFRQRLENLSDELADTIRIVEMPDKSTEDLVQLVSAGKIKYTICDELQAKSLK
                     RTFTNIDVSVPVGFTQQFSWGVNPEAGQLRDSLNAFLHDFLMSNDYWKIYRKYY"
     gene            4394..>5244
                     /gene="metA"
                     /locus_tag="PKH68_07685"
     CDS             4394..>5244
                     /gene="metA"
                     /locus_tag="PKH68_07685"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNX12857.1"
                     /translation="MPLNIPKTLPAVETLRKENIFVMDSERAQSQEIRPLKIVVLNLM
                     PLKITTETDLVRLLSNSPLQIEVDFLRLKSHTSKNTPIEHMMAFYKDLDEIQKSNYDG
                     MIITGAPVELLEFEEVKYWKELTGIFEWAHKHVTSTLYICWAAQAGLFHFYGVPKYPL
                     PEKMFGVFEHKTNHSLNPIFRGFDDVFYVPHSRHTEVRREDILKVPKLTLLSESEKSG
                     VYMVMARNGREFFITGHSEYSANTLDTEYKRDLKKGLPIKLPENYYRDNDPKNEPLVR
                     WRSHANLL"
BASE COUNT         1507 a         1081 c         1071 g         1585 t
ORIGIN      
        1 taaaaaaagc cgatacactt ttatgagcat atcggctttt cgggggattt tgaatcgaaa
       61 ggaacttatt cagaaaccac ttcgaatgag atttcagcgg aaacttcctt gtgaagtttt
      121 atagatgctg tataggaacc aatttctttg attggatctt tgagggcaat aattttccgg
      181 tcaacaataa ggccggcttt ttcgatggct tcggcaatct ggataggacc aactgcacca
      241 aagattttac ccgtagagct tgttttggca ccaattgtaa gagtgcccag tgctgcaatt
      301 ttttcgctga gttcaacggc gttgtttttg atttgctcaa atttatgtgc gcgttgtctg
      361 ttatcttctg ccagcatttt cttggcagat tcgcttgcga taacggcttt ttttgtcggt
      421 atcaggtagt tacgtccgta tccgtcttta accttaacaa tgtcaccttt gtaacccagg
      481 ttgattacat cttcttttaa tataatttcc ataatctttt ctccttttta ataattattt
      541 catcatatcg gttacataag gaagcagcga cagatgacgt gctcttttta ctgcctgagc
      601 aactttgcgt tgatatttca acgaagtgcc ggtgatacga cggggaagaa tttttccctg
      661 ttcgttgagg aattttttca agaattcggc atctttgtaa tcaatgtact tgataccgct
      721 ttttttgaaa cgacaatatt ttttcttttt aacgtcaacc gtgggtggag ttaaatatct
      781 gatttcgcct gaatgtgctg ccatgtttta attctccttt tcttctttaa gttctttttt
      841 tgctgctttt tcttctacca cttctttggg ttgtgatttt acattttttc ttttttcagc
      901 gtactcgaaa gcatacttat cgaggcggaa agttaagaaa cgaagtactc tctcatcccg
      961 acggaactga gtttcgaggg tagcgattac ggtcggttca acatcgaatt gtaacaaggc
     1021 ataaaatcct gtcgattttt tttgaattgg gtaagccagc ttgcgcatgc cccagttttc
     1081 ttcatttgtg atagttcctc cgttgtctgt gatgacagct ttgaacttat ctaccgcttc
     1141 cttcatctga acatcagaca aaacgggagt taaaatgaaa acggcttcat aatgatttaa
     1201 catactgttt tttacctttt taatggttaa taaatgattt ttattatgag ggtgcaaaga
     1261 tacaaacaat tctttgattg acaatagtat tcatggtttt ttattccgga ttagctgttt
     1321 ttttatccct gttgaagcaa tttaatatcc ggccggaagg accgaaacaa attcagttga
     1381 attaaatctg actttgaatt ttcgttataa attttcaatt ttacctttta taaccctgcc
     1441 attcagttta agctttacaa atttttgtat ctttgcggtt tattagtgct aaaccactca
     1501 ttgatgaaac gatacatact tatttttagt ctgattttag cgggaattca tgctttttca
     1561 cagactgatt cgatagcttc tcccaaacaa aaatcgacga aaccggtcaa acatataaag
     1621 gtgtggcata tatcagagca gcttgctgtg gctgatacta ttcccatgga tacggctacc
     1681 atcaactttc cggatcaaaa tccggtggaa acattcagta tagccaattc atatggtggc
     1741 aatctgggat taccgcttca gtccaaactt tacttcaaac gtccacaggg ctccgatttt
     1801 ctgtttgaca atgcttacaa gccttacacg ctcgatgttt cgtcagcttc attctacgat
     1861 actacttttc catttaccaa cctgacttac ctcaccggcg gttcggccta tcgcaaagag
     1921 gaacaggtca aatttttgtt tacggcaagc ccatcgggca aggccaattt cggaacaaca
     1981 ctggattata ttcatgcagt gggtgaatac aacaatcagg ctgcaaaacg attttcgggc
     2041 ggaattttcg gacggtacaa cggtaagcat tattcggcta acgggctggt agctgtcaat
     2101 aatcatttca actacgaaaa cgggggactg agcgatttga gttatctcac caatcccgat
     2161 ctgaatgtaa aaccgaaaga tattccggtg gccattaccg gatattccgt attccgcaaa
     2221 aatcagtttt attacaatca cggttacagc attggcttca accgggaagt aaaagtgagt
     2281 gcggattccg ttcggtcgga gtacgtaccg gtgacccggt ttgggcatac tattaagctt
     2341 gaggagatga aaaagcgata ctacgaacct accctggaga gaaagttcta cgaattttca
     2401 gatactaccc gaaagatagc caacgataca gcagcagttc gtactcttac gaacctggta
     2461 tcgatcagtc tggaagagga atttaataaa tggatgaaat tcggactgac cggatatgtg
     2521 gaaaatgaaa ttcagcaatt tacctaccaa ccagacacta cattgtatcg ggccacaaaa
     2581 tccaacaccc gggttggcgg tgtattgtca aaaaatagag gaaccaactt tcggtacagt
     2641 gttttgggag atatctacct gattggttac aagttgggcg aatttcgact ggaaggcaaa
     2701 gccgttggca atttcagact gttcaatcaa cagatttcgc tctcggccaa tgcatttatt
     2761 cggaatgagg aaccggcata ttaccttcaa cactattatt ccacccattt ccggtgggac
     2821 aacgaattta gcaaaatgta caaaacccat gtgagcggtt cattttcatt gccgaaattg
     2881 tatacacaat tgaatgtggg tgtcgagaat attacggatt acatctattt caacgaaaag
     2941 gcattgccgt cacaatatca gggcaatatt caggtacttt cggccgacct gaagcaggat
     3001 tttcattttg gtaaatttgt actcgaaaac aatgcggtgt atcaggtgag ttcagtgcca
     3061 gatgtgctgc cattaccagg tttgaccctg ttccataatt tttattatta ttccaaatgg
     3121 tttaaggatt tgtatccaca gttaggtgct tcggtgcgtt atcactccaa ttattatgca
     3181 ccggcttata tgccggccac cggccagttt tatacacaga aagaggtact tatcggaaac
     3241 tatccaattc tgaacgtgta tgccaatttt catctgaaac aggctcgatt cttcattgaa
     3301 tacaatcatt tgaacaaata ttttatgagt ggcgcacatt tttatatgcc caattatccc
     3361 attaatcctc cggtgatgaa gctgggactt tcctggaatt tttacaacta agcataaggt
     3421 gacttcgaat gaaacggtgt acgaaaataa atacaggcgt ggtgttggga ttgatagccc
     3481 tgatatgcat cggcgtaatt gtaactcaca aagtctatac accgacagta aaacgcgatt
     3541 tttcggaaat cagaaaatca gggcgactga gggtggtgac cgataaaagt tcgctgggat
     3601 ttaagttgta tgatgataaa atagaaggat ttaattacga actggcgaaa gcatttgcca
     3661 actcaatggg gcttgaactg gagataattc ccgagaacaa cctggattct gcattgaatg
     3721 gaataaaaga aaacaaatat gatttactgg caatgaatat tcctaacacg gcggaaacca
     3781 aaaaagaact ggcactcacc atacccattc tttcgggtcg ccaggtgctt gtgcagcagg
     3841 aaattattga taccacaaat ctgaaagcca ttcgaagtca ttatctgctg actaacgaca
     3901 gcatttgcat ccccgtccga tcacctttcc gacagcggct tgaaaacctg tccgatgaac
     3961 tggccgatac catccgaata gtggaaatgc ctgataaatc gacagaagat cttgttcagc
     4021 ttgtttcggc cggaaaaatt aaatatacaa tttgtgatga attgcaggct aaaagtttga
     4081 aacgcacttt tacaaatata gatgtctccg tacccgttgg atttacccag cagttttcgt
     4141 ggggtgtcaa tccggaagcc ggtcagcttc gcgattctct caatgctttt ttacatgatt
     4201 tcctcatgtc caacgattac tggaaaattt accgaaaata ctattgataa agttagaagt
     4261 cagaagtcag aagtcggaag tttgcagtta aatatttgat tcaaaacatc aaacatcaaa
     4321 catcaaacat caaacttgaa actataaacc tcacatttct aatttctatt tcctaatttt
     4381 taatttttca actatgccat taaatatccc caaaaccctt ccggcagtcg aaacattgcg
     4441 aaaagagaat atattcgtga tggattcgga gcgggcacaa agtcaggaaa tcaggccgct
     4501 gaagattgtg gtgttgaacc tgatgccgct caaaataacc actgaaacag atttggtacg
     4561 gttactttcc aattcgccgc ttcagattga ggttgacttt ctgaggttga aaagtcatac
     4621 gtccaaaaat acacccatcg aacacatgat ggctttttac aaggacctgg acgaaatcca
     4681 gaaaagtaac tacgacggca tgataattac cggtgctccg gtggaattgc tcgaattcga
     4741 agaggtgaag tactggaaag aactgaccgg tattttcgag tgggctcaca agcatgtcac
     4801 ctctacgttg tatatttgct gggctgctca ggcgggtttg tttcatttct acggcgtgcc
     4861 aaaatatccg ctgcccgaaa aaatgttcgg agttttcgag cacaaaacca accattcact
     4921 caatcccatt ttccgtggtt tcgacgatgt gttttatgtg cctcacagcc gtcataccga
     4981 agttcgcagg gaagatatcc tgaaagtccc taaactgacc ttgctttcgg aatccgaaaa
     5041 atcaggcgta tatatggtta tggcgcgcaa cggacgcgaa ttctttatca ccggacattc
     5101 ggaatattct gccaacaccc tggatacgga atataagcgg gacttaaaaa aaggattacc
     5161 catcaaactg ccggaaaatt attaccggga caacgacccg aaaaacgaac ctcttgttcg
     5221 ctggcgaagt cacgctaatc tgct
//
LOCUS       DAOPZK010000134         5222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_20020_length_5222_cov_27.507645, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000134 DAOPZK010000000
VERSION     DAOPZK010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20020_length_5222_cov_27.507645"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1927)
                     /locus_tag="PKH68_07690"
     CDS             complement(260..1927)
                     /locus_tag="PKH68_07690"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNX12858.1"
                     /translation="MKSDIDIAHEIELDRIKNVAHKLGINRESIISYGPYIAKIPISL
                     INKKKIRKSKLILVTAITPTKAGIGKTTVSIGLSLGLNKIGKVAAVALREPSLGPCFG
                     MKGGAAGGGYAQVLPMENINLHFTGDFHAITSANNMISALLDNYMYHHQDQADGIKQV
                     LWKRVLDVNDRSLRNVVTGLGGLTNGIPAETGFDITPASEIMAILCLAENEDDLKRRL
                     EKILLGYTFSGKPVTVKDLGIAGSIAVLLKDAMHPNLVQTTEHTPAFVHGGPFANIAH
                     GCNSVVATKMAMNFCEYVVTEAGFGADLGAEKFINIKCRKAGIQPVLSVLVVTTQALK
                     MHGGVPVKDIKLPNREGLQKGFENMDKHIQNLQSFGQQVLVSFNKYDFDTEEEIELLG
                     NHCKEMNVGFAVNDAFAKGGEGAVELAQKVVEMTSGKSDKTLHFTYKDTDTIQRKVEK
                     IAMKIYGARRVIFSEKALKMIPKILEMGGAEYPVCIAKTQYSFSDDERLYGAAKDFDF
                     HVNELVLNNGAEFIVAIAGSIMRMPGLPKEPQAANIDLINGEIVGLS"
     gene            complement(2019..3524)
                     /locus_tag="PKH68_07695"
     CDS             complement(2019..3524)
                     /locus_tag="PKH68_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HNX12859.1"
                     /translation="MKQAFRFMSADEAAAFINNGDIVGFSGFTAAGCPKDVPTAIAKR
                     ATAFHNEGKEFKIGMYTGASTGDSLDGALARAKAVSFRTPYQSNKDMRNALNSGEVAY
                     YDLHLSALALSLRYGFITPPKFAILEICDLTPEGEVVLTAGVGISPTIANLAEHIILE
                     WNHYHPKELNGFHDIYEASNPPSRREIPIYKPSDRIGTTTLKLDPKKILCVVETNQMD
                     EVGGFTPVDDVTARIGENVAAFLASELKKNRIPKEFLPLQSGVGNIANAVLASLGENK
                     DIPAFEMYTEVIQDSVIDLMKQGRVKFASGCSLTVSPEVLKEVYGNLDFFRDKIVLRP
                     QEISNSPEIARRLGLISINTALEADIFGNVNSTHVLGSKMMNGIGGSGDFTRNAYFSI
                     FTTPSTAKGGKISAIVPMVSHNDHSEHSVKVLITEHGIADLRGKSPIQRAHLIIENCV
                     DPAYRQLMYDYLKLGEKNGHTPINLAAGFGMHLQFAKSGNMLDTKWEDYTE"
     gene            3778..>5222
                     /locus_tag="PKH68_07700"
     CDS             3778..>5222
                     /locus_tag="PKH68_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX12860.1"
                     /translation="MNSNDIRNLEPKAIWENFYALTQIPRPSGKKEQIGQYLEDFGKS
                     LGLETLRDETGNVLIRKPATPGMEDRKAVVLQAHMDMVPQKNSHVSHDFEKDPIETRI
                     DGEWVTANETTLGADNGIGVAAAMAVMQSTGIPHPAVEMFITVDEETGMFGAFGLQPD
                     FLKGDILINLDSEDEGELYVGCAGGIDADITFKYKEVEVPEGDVAFKLSLTGLKGGHS
                     GVDIHLQRANANKLLFRFLKVAVAEYDARLCCFEGGSLRNAIPREAFSAVTVPADGAD
                     DLLELVAEYETLFQTEFEGVEDGIKFTAEKTDRVKGLMPEEIQDDLINAVTACPNGVF
                     RFIPELPDVVETSNNLAIIRSDGSSITACCLIRSSVESRKEELASMVESAFTLAGAKV
                     EFKGGYPGWKPNLASPILKEMTNIYEKNFGKTPRIMIIHAGLECGILGTHYPNMDMIS
                     FGPTIRFPHSPAEKVNIPTVQKFWDFLLHTLA"
BASE COUNT         1420 a         1182 c         1155 g         1465 t
ORIGIN      
        1 cgtccgttcg agtcgggcct ccgcaacata gaagaaggaa aattgtaaaa agcagttttc
       61 cttttttttt atttcaataa aaaatttcct tcggggctcg tcgaggttca taccccgata
      121 acaatgggac gttttgttaa agtcgggcat ccacaataaa gaaaaaggga aattgtaaaa
      181 agcagttttc ctttttttat ttcaataaaa aaatccgaaa ctatcaaagc cccggatttc
      241 agttacagat aagttgctgt taactcaatc ccactatttc accgtttatt agatcaatat
      301 tagcagcctg gggttctttg ggcagaccgg gcatacgcat gatgcttccg gcaatggcaa
      361 caataaattc agctccgtta ttcaatacca gttcattcac atggaagtca aaatctttgg
      421 ctgccccata caaacgttcg tcgtctgaaa aggagtactg cgtcttggca atacaaacgg
      481 gatattcagc tccacccatt tccagtattt tcggtatcat cttcagggct ttttcggaaa
      541 atatcaccct cctggctcca tatatcttca tggctatttt ttctacttta cgctgaatgg
      601 tatcggtgtc tttataggta aaatgtaaag tcttatcgct ttttccactt gtcatttcca
      661 ccaccttctg agccagttca acagctcctt ccccgccttt agcgaaagcg tcattcaccg
      721 caaatcctac attcatttct ttacaatgat taccaagcaa ttctatttct tcttcggtat
      781 caaaatcata cttgttgaat gaaacaagta cctgttgacc aaaagactgc aaattctgaa
      841 tatgtttatc catattttca aaacctttct gtaagccttc cctgtttggc agtttaatat
      901 cttttaccgg tacgccgccg tgcattttca gtgcctgagt tgtaaccaca agtaccgaca
      961 acaccggctg aatacctgct ttccggcatt tgatgtttat aaatttttca gcacccaggt
     1021 ctgcgccaaa accggcttcg gtcactacgt actcgcaaaa attcattgcc attttggtgg
     1081 caactaccga gttgcagcca tgtgctatat tggcaaacgg gccgccatgt acaaaggcgg
     1141 gggtgtgttc ggtggtctgc accaggttgg gatgcatggc atccttgagc agcacggcaa
     1201 tggaacctgc aatgcccagg tctttgaccg taacaggttt tccggaaaag gtatatccca
     1261 gtagtatttt ttccagtcgg cgtttgagat cgtcttcatt ttcagccaga caaagtatgg
     1321 ccattatttc ggaagcggga gtaatgtcaa atcctgtttc ggccggaata ccgttggtga
     1381 ggcctcccaa tccggtaacc acattcctca gactgcggtc gttcacatcc agtacgcgtt
     1441 tccagagaac ctgtttgatt ccatcagcct ggtcctgatg atggtacata taattgtcga
     1501 gcaacgcgga aatcatattg ttagccgacg tgatggcatg aaaatcgccc gtaaaatgca
     1561 ggttgatgtt ttccataggc aacacctgag cgtatccacc tccggcagcg ccgcccttca
     1621 tcccaaaaca gggaccaagc gaaggctcac gcagcgcgac agccgccact tttccgattt
     1681 tattcagtcc aaggctaagc ccgatggata cagtggtttt tccaatccct gctttggtag
     1741 gagtaatagc tgttaccaat atgagtttgc ttttccttat tttcttttta ttaatcagac
     1801 tgataggaat ttttgcaata tacggaccgt aggaaataat cgattcccgg tttatcccca
     1861 gcttgtgagc tacatttttt atccggtcga gttctatttc gtgagctata tctatgtctg
     1921 atttcatatt tatctttttt ttaattggtt ggttttaact ttaaaaacaa aagagacccg
     1981 aaaaagtctc ttttgctggt aaatatttca gaagaaactt attcggtata atcttcccat
     2041 ttggtatcga gcatattgcc acttttggca aattgcaggt gcataccaaa tccggctgcc
     2101 agattgatgg gagtatgacc attcttttcg ccaagtttga ggtaatcgta catgagctga
     2161 cggtaagccg gatcaacaca gttttcgatg ataaggtgag cacgctgaat gggtgatttt
     2221 cctctcaggt cagcaatacc atgttcggta atcagtactt ttacggagtg ttcagagtgg
     2281 tcattgtgcg aaaccatggg tacgatagca ctgatttttc cgcctttagc agtagatgga
     2341 gtggtgaata tggagaaata ggcattacgg gtaaagtctc ccgatccgcc aattccgttc
     2401 atcattttgc tgccaagtac atgggtactg tttacatttc cgaaaatatc ggcttcgagc
     2461 gctgtattga tggaaatcag acctaagcga cgtgctattt cggggctatt ggagatttcc
     2521 tgcggacgaa gcacaatttt atcgcggaag aaatcgagat ttccatacac ctctttcaga
     2581 acttcgggcg aaacagtgag cgaacaacca cttgcaaact tcacgcggcc ttgtttcatc
     2641 agatcgataa cggagtcctg aatcacttcg gtgtacattt cgaaagcggg aatatcctta
     2701 ttttcgccga gcgaagccag tacagcattg gcaatattgc ctacacccga ctgaagtggt
     2761 aaaaactctt tcggaattct attctttttt aattcagatg caaggaaagc agccacattc
     2821 tcgccaatac gcgccgtaac gtcatctacc ggagtaaatc cgcccacttc gtccatctga
     2881 ttggtttcca ccacacacag aattttttta gggtccagct tcagtgtagt cgttccaatg
     2941 cggtcggatg gtttgtatat cggaatttcg cggcgtgacg gcggattgga ggcttcataa
     3001 atatcgtgga atccattcaa ttcctttgga tgataatgat tccactccag aattatgtgt
     3061 tcagccagat tggcaatagt gggcgaaatg ccaacaccgg cagtgagcac cacttctccc
     3121 tccggggtaa ggtcacatat ttcaagaatt gcaaatttgg gaggtgtaat aaatccatac
     3181 cgcaaactca gcgccagtgc cgacaagtga aggtcgtagt aagctacttc accgctgttg
     3241 agggcatttc gcatatcttt attggactga tagggtgtgc ggaaactcac agctttggca
     3301 cgggcaagag caccgtcgag cgaatcgccg gtagatgcac cggtgtacat gccgattttg
     3361 aattctttcc cttcattgtg aaatgcagtt gctcttttgg ctatcgccgt aggcacatct
     3421 ttggggcaac ccgcagcggt aaatccgcta aaaccaacga tatcgccgtt gttgataaaa
     3481 gctgctgctt catcggcgga cataaatctg aaagcttgtt tcatactgtt ttatatgttt
     3541 atttgatatg tatttttgaa ttccttttga tagtgcaaaa ttatcatttt ttttccaaca
     3601 atcaaataca taaataaata agggaaaaat ggatgtcagc atcagacaat gttgaaagaa
     3661 acttcaataa ttataaattt aatttacaaa acataaattt gtcgggataa atgttgatat
     3721 ttgtagttat caaaaaccaa actgaaaaca tctaacaata ttaaaaatta tagaactatg
     3781 aattcaaacg acattcggaa tttagagccc aaagccattt gggagaattt ctatgcgctg
     3841 acacaaattc cacgtccatc cggaaaaaaa gaacagattg gacaatatct cgaagatttc
     3901 ggcaaatcgc tcgggttgga aacactgcgc gacgaaacag gtaatgtgtt gatacgcaaa
     3961 ccggccactc cgggcatgga agaccgcaaa gcggtggtac ttcaggcaca catggatatg
     4021 gtaccacaga aaaacagtca tgtaagtcat gattttgaaa aagatccgat tgaaacacgc
     4081 attgatggtg aatgggtgac agctaacgag accactttgg gtgccgacaa cggcattggt
     4141 gtggcagctg caatggctgt gatgcagtca accggcattc cccatccggc tgtcgaaatg
     4201 tttattaccg tagatgaaga aaccggcatg tttggtgcat ttggcctgca gcccgatttt
     4261 ctgaaaggag atatccttat caatctcgac tctgaagacg aaggcgaatt gtatgttggc
     4321 tgcgccggcg gtattgatgc cgatattaca ttcaaataca aggaagtgga agttcccgaa
     4381 ggggatgttg ctttcaaatt gtcgctcaca ggtctcaaag gaggccattc gggtgtggat
     4441 attcatctgc aacgtgcgaa tgccaataag ttgctcttcc ggtttctgaa agtagctgtg
     4501 gccgaatatg atgcccggct ttgctgcttt gaaggaggtt cgctccgcaa cgccatcccg
     4561 cgcgaagcat tttcggccgt taccgtacct gctgatggag cagatgatct gctggaactg
     4621 gtagccgaat acgaaacact tttccaaact gaatttgaag gcgtggaaga tggaataaag
     4681 tttacagccg aaaaaaccga cagagtgaaa ggtctgatgc ccgaagaaat tcaggacgac
     4741 cttatcaatg cggtaacagc ctgtcccaac ggagttttcc gctttatccc cgaacttccc
     4801 gacgtggtgg aaacatccaa caacctggcc atcattcgtt cggacggcag cagcattacc
     4861 gcctgttgtc tgatacgcag ctcggtggaa agccgcaagg aggaactggc ttcgatggtg
     4921 gaaagcgcct ttacactggc tggcgccaaa gtagaattca agggtggcta ccccggatgg
     4981 aaaccaaacc tggcgtctcc tattctgaaa gagatgacca atatctacga aaagaatttt
     5041 ggcaaaacac cgcgtatcat gattattcac gccggactgg aatgtggtat tctgggcaca
     5101 cactacccga acatggacat gatttctttc ggacccacca tccggttccc gcactcaccg
     5161 gccgaaaaag tcaatatccc gacagtacaa aaattctggg attttctgct tcacacgctg
     5221 gc
//
LOCUS       DAOPZK010000135         5169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_20298_length_5169_cov_21.824404, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000135 DAOPZK010000000
VERSION     DAOPZK010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20298_length_5169_cov_21.824404"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /gene="ubiE"
                     /locus_tag="PKH68_07705"
     CDS             <1..728
                     /gene="ubiE"
                     /locus_tag="PKH68_07705"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNX12861.1"
                     /translation="NNVKPYNQEEEKTRQLNRMFNTISRQYDKFNDIMSMGLARRWRK
                     KALLTLKDYNPENILDIAAGTADICIKSFEYLRPRNVTGIDISEKMLEIGKKKVAEAG
                     LSDRVTLRVEDVSSMSFADQSFDAATTSFGIRNFEKLDQSLLEIHRVLKPHGKFVILE
                     MSEPQTPVIKQGYLLYTKTLIPFAVRTFANDPKAYSYLTNSMSAFPQGKVFIDILKKN
                     GFNCLKYKTFFLGVCSLYLAEKE"
     gene            882..4925
                     /locus_tag="PKH68_07710"
     CDS             882..4925
                     /locus_tag="PKH68_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX12862.1"
                     /translation="MKKIQLALLLSCIVAQNFAMSPTLKGFAYADEPAPAGNEWESPE
                     KLALNKEQPRAWFFSFQDTESARKVLPENSAYWKSLNGTWKFNWVKTPELRPKDFYKP
                     EYDVTGWDNTPVPSNWNIVGIQKNGDLKYGVPIYVNQQVIFKHQVKPGDWRGGVMRTP
                     PADWTTYEYRNEVGSYRREFEIPENWKGREVFISFDGVDSFFYLWINGQYVGFSKNSR
                     NAARFNITKYLVKGKNTVAAEVYRNSDGSFLEAQDMYRLPGIFRTVALYSTPRVQIRD
                     LQVIPDYQNGTGSLDVTVNLRNLNSKKAALTNYKIRYSLFANKLYSDETYFVKSSEVP
                     VGAEKAKVVLNPNEQINLKKSVLNYGDIKPWSAEEPNRYVLVAELMDKKGRTVETVSA
                     YVGFRKVEIKDTKAEDDEFGLAGRYFYVNNKPVKLKGVNRHESSPETGHAISRERMTE
                     EIMIMKRANINHVRNSHYPDDPYWYYLCNKYGIYLEDEANIESHQYYYGEASLSHPAE
                     WQAAHVARVLEMAHSNVNNPSIVIWSLGNEAGPGKNFVAAYNELKAFDPSRPVQYERN
                     NDIVDMGSNQYPSIGWMYGAVKGKYDIKYPFHVSEYAHAMGNACGNLVDYWKAIESTN
                     FFCGAAIWEWIDQSMLYYDKITGTPFFTYGGDFGEKPNDGQFIFKGVVAADLSPKPHY
                     YEVKKVYQNIAVDSVNAETGTFNVFNKNYFTDLSAYNLRWSLYEDGIETKAGNIALGT
                     IAPRTRQKITVPYQFDGLKPESEYFVKFQFELKNDMPWNRKGYVQADEQYSVKAAVNV
                     PSVLTAASAVTTEKPEIEETSATIKTVKGKNFRVEFDTTTGTIYSLTYGNQTIIAAGN
                     GPKLDAFRAYTNNDNWAVDKWYENGLHNLKHKAISNSFVKKADGTVVASFTVVSQAPN
                     AAQLLGGTTATRNSVLELTDRKFGENDFKFTTNQLFTIYPDGSIELQANVNSNQPALA
                     LPRLGYVMKIPQRYSEFTYYGRGPIDNYNDRKSSQFIEIHKNTVKDEFAPIARPQNVG
                     NHEDVRWAALTDKSGNGAVFVAADRLSVTALQYTANELVAAAHPYELPKPGDTYLHLD
                     MGVTGLGGNSCGQGGPLEHDRVKADNNSVGFIIRPAGSDLTKTAAVRSAGEMPIAITR
                     SKSGLVKLTSARKNAVICYTTDAKGKEKIYTEAIPMRDGGTVTAWYKENPQLKTSISF
                     PKIESVQSEVIFTSSEEAGEGDATNLTDGDPNTIWHTMYSVTVAKHPHWVDLDAGDVK
                     TIKGFTWLPRQSGTNGDVKDYTLQLSLDGKNWGEPVARGKFDNGKAEKKVLFDKPVKA
                     RYIRFTALSEQRGQDFATGAEMTILAE"
BASE COUNT         1618 a         1171 c         1156 g         1224 t
ORIGIN      
        1 ttaacaacgt aaaaccatat aaccaggaag aggaaaaaac ccgtcagctg aaccgcatgt
       61 tcaacaccat atcccgtcag tacgacaagt tcaacgacat catgagcatg ggacttgcgc
      121 ggagatggag aaagaaggct ctgcttaccc tgaaagacta taatcctgaa aatatactcg
      181 atatcgcggc aggaacagcc gatatatgca tcaaatcatt cgaatatctt cgtccccgta
      241 acgtaaccgg gattgatatt tcggaaaaga tgctcgaaat cggaaagaaa aaagttgcag
      301 aagccggact gagcgaccgt gttactcttc gcgtggaaga tgtaagcagc atgagttttg
      361 ccgatcagag tttcgatgcg gcaaccactt cgtttggcat ccgtaatttt gagaaacttg
      421 atcagagttt gctggaaata caccgggtgc ttaaaccaca cggcaaattc gtaattcttg
      481 aaatgagcga accgcagacg ccggttatca aacagggata cctcctgtac accaaaaccc
      541 tcataccttt tgctgtgcgt acatttgcca atgaccccaa agcatactca tatctcacca
      601 actcgatgag tgcattcccc cagggaaaag tttttatcga tattctgaag aaaaatggtt
      661 tcaattgtct gaaatacaaa acgttctttt tgggagtttg tagcttatat ctggcagaaa
      721 aagaataaac agtcaaaagt tatcggttct cgcaggcatt ttattttacc aaaaataatc
      781 tgtatttttg tggacaaaac ctgaatgcgg gattgcagga gtaagttatc acctgcagtc
      841 aaaaaaatct actataaatc atactataac caatcacaaa catgaaaaaa atccaattag
      901 cacttttgct aagttgtatc gtggcgcaaa actttgccat gagcccaact ctcaaagggt
      961 ttgcctatgc cgacgagcct gctcctgcag gcaacgagtg ggaatcgccc gaaaaacttg
     1021 cactcaacaa agaacaaccg cgagcctggt tcttttcctt tcaggatacc gaaagcgccc
     1081 gcaaagtact ccccgaaaat tcagcatatt ggaaatcgct caacggaacc tggaaattca
     1141 attgggtgaa aacgcccgaa ctccgtccga aagatttcta caaacccgaa tatgacgtaa
     1201 ccggatggga taatactccc gttccttcga actggaacat tgtgggcatt cagaaaaacg
     1261 gtgatctgaa atatggtgtg cctatctatg taaaccaaca ggttattttc aaacatcagg
     1321 taaaaccggg cgactggcgc ggtggtgtaa tgcgtacgcc tcctgccgac tggaccactt
     1381 acgaataccg caatgaagtg ggttcatacc gccgtgaatt cgaaattccg gaaaattgga
     1441 aaggacgcga agtatttatc agcttcgacg gcgtggattc attcttttat ctgtggatca
     1501 atggtcagta tgtgggattt tcgaaaaatt cacgaaatgc tgcccgtttc aatatcacaa
     1561 aatacctggt gaaaggaaaa aacaccgtgg ctgctgaagt gtaccgcaat tccgacggct
     1621 cgtttctcga agcacaggat atgtaccgcc tgccgggcat cttccgcact gtagccctct
     1681 actctactcc cagggtacaa attcgtgact tgcaggtaat tcccgattat cagaatggta
     1741 caggaagtct ggacgtaacc gtcaatcttc ggaacttgaa cagtaaaaaa gcggcattaa
     1801 cgaattacaa aataagatat tcattatttg ccaataaact ttactcggac gaaacctact
     1861 ttgtaaaaag ttctgaagta cctgttggag ctgaaaaagc caaagtcgtc cttaatccca
     1921 acgaacaaat taatttaaaa aaatcagtac tgaattacgg agatattaaa ccctggtcgg
     1981 ccgaagaacc caaccgctat gtgctggtag ctgaattgat ggataaaaaa ggtcgtacag
     2041 tagaaacggt atctgcatac gttggtttcc gcaaagtgga aatcaaagac actaaagctg
     2101 aagacgatga attcggactg gcaggccgtt atttttatgt aaacaataaa ccggtgaaac
     2161 tgaaaggcgt taatcggcac gagagttctc ccgaaaccgg tcatgccatc agtcgcgaac
     2221 gaatgacaga agagatcatg atcatgaaac gcgccaatat caatcatgta cgaaattcgc
     2281 attatcccga tgatccatac tggtactacc tctgcaataa atacggtatc tacctggaag
     2341 atgaagccaa catcgaatcg catcagtact attatgggga agcatcactt tctcaccctg
     2401 ctgaatggca ggcagctcac gtggcacgcg tactggaaat ggctcacagc aatgttaaca
     2461 atccatccat agtgatctgg tcactgggta atgaagccgg tcccggtaag aatttcgtag
     2521 ctgcctacaa cgaactcaag gcgtttgacc cctcgcgtcc tgtacaatac gagcgcaaca
     2581 acgacattgt ggatatgggt tccaaccagt atccgtccat aggctggatg tatggtgctg
     2641 tgaaaggaaa atatgatatc aaatacccgt tccatgtttc ggaatatgca catgccatgg
     2701 gcaatgcctg cggcaatctg gtggattact ggaaagccat cgaatcgacc aatttcttct
     2761 gcggagctgc catctgggag tggattgacc aaagtatgct ttactacgac aaaataaccg
     2821 gaaccccatt ctttacgtat ggcggtgatt ttggcgaaaa accaaacgat ggacagttta
     2881 ttttcaaggg agtggtagct gccgatttat cgcccaaacc gcattactac gaggtaaaaa
     2941 aagtatatca gaatatcgcc gttgactcgg ttaatgccga aaccggaaca tttaatgtat
     3001 ttaacaaaaa ctatttcacc gatctttcgg cctacaatct gaggtggtca ctctacgaag
     3061 atggaataga aacgaaagca ggaaatattg ctttagggac aattgctccg cgtacccggc
     3121 aaaaaatcac agttccttat cagtttgacg gattgaaacc cgaaagtgaa tattttgtaa
     3181 agtttcagtt tgaactgaaa aacgacatgc cctggaacag gaaaggttat gttcaggccg
     3241 atgaacaata ttcggtgaaa gcagctgtga atgttccttc tgtactgaca gcagccagtg
     3301 cagttacaac tgaaaaaccg gaaatagaag aaacatcagc cacgataaaa accgtgaaag
     3361 gtaagaattt cagggtggaa tttgacacta caaccggaac catttacagc cttacctacg
     3421 gcaaccaaac catcatagcc gccggaaacg gacctaaact ggatgccttc cgtgcttaca
     3481 ctaacaacga caactgggca gtggataaat ggtatgaaaa cggactgcac aacctgaaac
     3541 acaaagcgat aagcaatagt tttgtaaaaa aagccgatgg cacagttgta gcttcgttca
     3601 ctgtggtgtc acaggcaccc aatgctgcac aacttctggg aggaactacc gccacacgta
     3661 atagcgtgtt ggaactcact gatcgaaaat tcggcgaaaa tgatttcaaa ttcactacca
     3721 atcagctgtt taccatttat cccgatggtt ccattgaact gcaggccaat gttaattcaa
     3781 atcagccggc tttggcgctt ccacggttgg gttatgtgat gaaaattcca caacgttatt
     3841 ctgaatttac ttattacgga cgtggcccga ttgataacta taacgaccga aaatcaagtc
     3901 agtttatcga aattcataaa aacaccgtaa aagacgaatt tgcacccatt gcccgtccgc
     3961 aaaatgtcgg caatcatgaa gatgtacgct gggcggcgct gacagacaaa tcaggtaacg
     4021 gagctgtatt tgtggctgct gaccgcctat cggtaaccgc actgcaatat acagctaatg
     4081 aactcgttgc agctgcgcat ccgtatgagt tacccaaacc gggagatact tatttgcacc
     4141 tggatatggg tgttacaggt ctgggaggca acagttgcgg tcagggtggt ccactggaac
     4201 acgacagagt gaaagccgac aacaattcgg tagggttcat tatccgccct gccggaagcg
     4261 atttgaccaa aacagcggca gtacgcagtg ccggtgaaat gccgattgcc atcacccgca
     4321 gcaagtccgg tttggtgaaa ttgacatcag ccagaaaaaa tgctgtgata tgttatacaa
     4381 ctgacgcaaa aggaaaagaa aaaatttata ctgaagcaat tcccatgcgt gatggtggaa
     4441 ctgtaactgc ctggtataaa gaaaatccac aactgaaaac atccatttct tttccaaaaa
     4501 ttgaaagtgt gcagtcggaa gtgatcttta caagcagtga ggaagccggt gaaggtgacg
     4561 ctaccaacct caccgacggt gatccgaata ccatctggca taccatgtac tccgttaccg
     4621 tagccaaaca tccgcactgg gtggacctcg atgcaggtga tgtgaaaacc atcaagggtt
     4681 tcacctggct gccgcgtcag agtggcacta acggcgacgt gaaagactac accctacaac
     4741 tgagcctcga cggtaaaaac tggggcgaac ccgtagcgcg tggtaagttc gacaacggca
     4801 aagccgaaaa gaaagttctc ttcgacaaac ccgtcaaagc ccgttacatc cgctttaccg
     4861 ccctgagcga gcaacgcgga caggattttg ctaccggcgc cgaaatgacg atattggctg
     4921 aataagacta atccctaccg gatacaatcc ccgccggata aacaaactaa taccccggaa
     4981 gttctcagta actttcgggg tttttttttt acagggatga atgaaaggac tgaggaatga
     5041 tgagttgatt aagttttgct acagatatga agttttaata taacaagcca gatcaataca
     5101 aatagaaata aaagtaataa tcttcagcaa tcagtgctct ccctccctgg agggagccgg
     5161 agggaggtc
//
LOCUS       DAOPZK010000136         5076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_20835_length_5076_cov_22.637124, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000136 DAOPZK010000000
VERSION     DAOPZK010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20835_length_5076_cov_22.637124"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PKH68_07715"
     CDS             <1..1336
                     /locus_tag="PKH68_07715"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNX12863.1"
                     /translation="KTNNQQKPECTFFWFRRDLRLHDNAGLFEVLKQSNSVIPLFIFD
                     TEILDKLPDREDKRVVFIHQTLSAMHSELLKIGSSLLVMHGSPLEVFQKLLEAYSVAS
                     VYANTDYEPETIARDAQISDLLRANGVAFRLLKDQVIFEKSEIVKDDGSPFEIFTPYS
                     KAWKHKFSQTNLETFPSGDLQHHFQKTNPLPFPSLSEIGFNYIETGISDPFIDKNIIQ
                     HYHVTRNFPYLPGTSKLSVHLRFGTISIRELVRIAADLNEQYLNELIWREFFMMILYH
                     HPRVTHESFKKKYDYIAWRNDEDEFHNWCMGETGYPIVDAGMRELNETGFMHNRVRMI
                     VASFLTKHLLIDWRWGEAWFAAKLLDYELSSNNGNWQWSAGCGCDSAPYFRIFNPHEQ
                     LRKFDPEKIYTQKWVKNLHQPDYPAPIVEHTTARERCLSTYKKALDNFKNER"
     gene            1333..1776
                     /locus_tag="PKH68_07720"
     CDS             1333..1776
                     /locus_tag="PKH68_07720"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNX12864.1"
                     /translation="MKPQTEIRDFIRQVLSANRFAVLATVSEGKPHSSFVAYTASGDS
                     LQLIFATYRHTRKYANLKQNNQVSMLIENSKDRSTEITVLTATGKAFEVDTAHGEALL
                     QTHLRQHPELNDFLLSPDCAVFRVDVEAYQLVLGIDDIRWWKTAE"
     assembly_gap    2267..2366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2837..3583)
                     /locus_tag="PKH68_07725"
     CDS             complement(2837..3583)
                     /locus_tag="PKH68_07725"
                     /inference="COORDINATES: protein motif:HMM:NF040365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6261 family protein"
                     /protein_id="HNX12865.1"
                     /translation="MKIRTIIYRTSNESLAAVALRVIDTIEKSISAEAKQSLLFSRLM
                     EVNNRYQSSVEPNGSKKTAEAIEAKFKERERLYTEFYDLTYGLQRSRVALQKEAAQKV
                     FPVLNMYGKSFKQERIADQTVRYIRIIETLKGADYTAAIAALNATELFAELSEAQTMY
                     EDLYLGRGNRLSLKVPTVEMRKEMNNALKDMVDEIEINEKKYPSEANTALKINIEQRF
                     NEVYIPASVGKKDRQPSETETTKPSETLVS"
     gene            complement(3708..4169)
                     /locus_tag="PKH68_07730"
     CDS             complement(3708..4169)
                     /locus_tag="PKH68_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12866.1"
                     /translation="MNNLLNLLTRLVLTFVKTRGGRNVVLADSANSQPVKSRNRKIRF
                     VKKQDKIPLKSLRKVRYYELHDEVISIDIDNYISDFSDTLDYQYSLYSRKIKSLLYQL
                     CYRLSKKYSIGIKRWNAALHDAITLLFGLSNAHPSYIFFAIFGSSSFRIQA"
     gene            4852..>5076
                     /locus_tag="PKH68_07735"
     CDS             4852..>5076
                     /locus_tag="PKH68_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12867.1"
                     /translation="MIISKIFHRNEWRICINVGYDNEIVAKIKQLPDAKWSRTLKAWH
                     IPYTREAFDQLKTLFPDVEYQNTSTELDTEQ"
BASE COUNT         1507 a         1069 c         1000 g         1400 t
ORIGIN      
        1 gaaaaccaac aaccagcaaa aacccgaatg cacctttttt tggttccggc gcgatcttcg
       61 gctgcatgac aatgccggct tattcgaagt attgaaacag agcaattcgg tgatacctct
      121 gtttattttc gacacggaaa tacttgataa attgcccgac agggaagaca aacgggtggt
      181 ttttattcat caaacccttt cggctatgca cagcgaactt ttgaaaatcg gcagttccct
      241 gcttgtaatg catggcagcc cgctggaggt ttttcagaaa ttgctggagg catactctgt
      301 cgcatcggta tatgccaata ccgattacga accggaaacg atagcgcgtg atgctcaaat
      361 ctctgatttg ctgagagcca acggagttgc tttcaggcta ctgaaagacc aggtgatatt
      421 cgaaaagtcg gaaatagtaa aagacgacgg cagcccgttc gagatattta ccccttattc
      481 caaagcctgg aagcataaat tctcacaaac aaacttagag acatttccca gcggggattt
      541 gcagcatcat tttcaaaaaa caaatcccct gccctttcca tcactcagcg aaattggttt
      601 taattacatt gaaaccggca tttcagaccc atttatcgat aaaaacataa tccaacatta
      661 tcacgtaacc cgcaatttcc cctacctgcc gggaacttcg aaattgagcg tgcatcttcg
      721 ttttggcacc atcagtatca gggaactggt gcgaatagca gcagacctga acgaacaata
      781 cctgaacgaa ctgatatggc gcgaattttt catgatgatc ctgtatcacc atccgcgcgt
      841 aacccatgaa agtttcaaaa aaaaatacga ttacatcgcc tggcgaaacg acgaagatga
      901 atttcacaac tggtgcatgg gcgaaaccgg ttatccgata gtggatgccg gtatgcgcga
      961 actcaatgaa accggcttta tgcacaaccg ggtgagaatg attgtggcaa gttttctgac
     1021 caagcacctg cttatagact ggcgctgggg cgaagcctgg tttgcagcga aactgctgga
     1081 ttatgagtta tcgtccaaca atggcaactg gcaatggtcg gctggatgtg ggtgtgattc
     1141 tgctccctat ttcaggatat ttaatccaca cgaacagctc cgaaaatttg atcccgagaa
     1201 gatatacacg caaaaatggg tgaaaaatct ccatcaaccg gattatcccg cacccatcgt
     1261 tgagcacact acggccagag agcgctgcct cagcacctac aagaaagccc tcgacaactt
     1321 caaaaacgag cgatgaaacc acaaactgaa atccgggatt ttatccgcca ggtactcagt
     1381 gccaatcgct ttgcagtatt ggccaccgta agcgaaggta agccacacag cagttttgta
     1441 gcctatacag cctccggtga ttcactgcaa ctaatctttg ccacttaccg ccacacgcgt
     1501 aaatatgcca acctgaaaca aaacaatcag gtttccatgc ttattgaaaa cagcaaagac
     1561 aggagtacgg aaatcaccgt attaaccgca acgggaaaag cctttgaggt ggatacagcc
     1621 cacggtgaag cactgttaca gacgcatctc aggcaacatc ccgagcttaa cgacttcctg
     1681 ctttcacctg actgcgctgt gtttcgggta gacgtggaag cctaccagct tgtgctggga
     1741 attgacgata tacgctggtg gaaaaccgca gaataaagta aataaaagtc tccgtgtgat
     1801 attgccactc ccccacttaa ttttgagaag taacttagcc ctgccaatat aatccacaca
     1861 gcaatttccg ggcacactac agaacgtttt tttcagccac agcatgatcg gttattgcat
     1921 tcggtgtgtg atgccgcaaa aattatatca actaacgatt attgcacttg tatatttcat
     1981 ttgaattgcc ttatacacct ccttaaaccg ggttttcaac aaataaatat tacaatgacc
     2041 gtccgtgagc acatgatttg tcaaatacaa attacaatga ccgtccgtgt gcacatgatt
     2101 agtcaaatac aaattacaat gaccgtccat gtgcacatga ttagtcaaat acaaattaca
     2161 atgaccatcc gtgagcacat gattagtcaa atacaaatta caatgaccat ccgtgagcac
     2221 atgattagtc aaatacaaat tacaataacc gttcgtgagc acatgannnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnncgtg agcacatgat tagtcaaata caaattacaa
     2401 taaccgttcg tgagcacatg attagtcaaa tacaaattac aataaccgtt cgtgagcaca
     2461 tgattagtca aatacaaatt acaataaccg tccatgaaca catgattagt caaatacaaa
     2521 ttacaatgac cgtccgtgag cacatgatta gtcaaataca aattacaatg taaatttcct
     2581 ataaaaggag actgtccggg aaactgtgca aacaggaaaa tgcattattt tcatatcacc
     2641 gaaaaaaaag acctgaaaga accgaaagaa agcgactgtg atgttcattc cgatatgaat
     2701 tatcatccta cattttcagg ataatgcgga ctaaaaatca tagtccggtt tgcataaacg
     2761 ggctgaagta tccacaaaaa aaactccgca ggccatcctg cggagtctca tttaatgaac
     2821 atcgatcaga acggtattac gataccagtg tttctgaggg ttttgttgtc tctgtttctg
     2881 acggttgccg gtctttttta cctacggagg caggaatata tacttcgttg aaacgttgct
     2941 caatgtttat cttcagtgca gtgttagctt ccgaaggata ttttttttca ttgatttcta
     3001 tttcgtccac catatccttg agggcattgt tcatttcttt gcgcatctct acggttggca
     3061 ctttcagcga tagcctgttg ccacgtccca ggtacaagtc ttcgtacatt gtttgtgctt
     3121 cagagagttc agcaaagagc tccgttgcat tcagagctgc aatagcagcg gtataatctg
     3181 ctcccttcag cgtttcgatg atacgaatgt aacgtaccgt ttggtcggct attcgctctt
     3241 gtttaaatga cttaccatac atattgagca caggaaaaac cttttgtgct gcttccttct
     3301 gaagtgctac cctcgaccgc tgaagcccgt aagtcaaatc atagaattcg gtataaagtc
     3361 tttcccgttc tttgaactta gcctctatag cttctgctgt ttttttactg ccgttgggct
     3421 ccaccgaact ctgataacgg ttattaactt ccatcaaccg ggagaataat aaactttgtt
     3481 tggcttctgc cgaaatagat ttttctatgg tgtcgatgac acgcaatgca actgccgcaa
     3541 gagattcgtt actcgtacgg tatattattg ttcttatttt cattgtgaaa ttgtaatatt
     3601 tatttattta tttaatcgaa aaaatatagt tttaagctgc aggccaaacc ctgcaagcct
     3661 tagtatacgt cccgaagatt tggaggacac gaacggatta aataaattca ggcttgaata
     3721 cggaaggaag atgagccaaa aatggcaaaa aatatgtagg atggatgagc attgctcaat
     3781 ccgaaaagca gcgtgatggc atcatgcagg gcagcattcc accgctttat accgattgaa
     3841 tattttttgg aaagcctgta acataactgg tatagcaggc ttttgatttt acgggaatat
     3901 agagagtatt gatagtctaa tgtatccgaa aaatcggaaa tataattgtc gatatcgatg
     3961 gagattacct catcatggag ttcataatac ctgactttac gaagcgattt gaggggtatc
     4021 ttgtcctgtt ttttaacaaa ccggatcttc ctgtttcttg attttacggg ctgtgaattt
     4081 gcactatcgg ctaacacgac attccggcca ccccttgttt tcacaaaggt cagtaccagt
     4141 ctggtcagaa gatttagcaa gttgttcatt gagcctgttt taagtattgt ttaatttacc
     4201 ctacatttct atacaaaaac cgtgccagtg ttaacttctg ccaaaatgtc tgactttttc
     4261 tttctacacc tgacaaaact gtcaggtttg ttctgatttt tgtcaggttt ggcagactat
     4321 taacaacccg gggtgtaaac cggagtctgc ggggagatag tttctttaca gtatgccgtt
     4381 gtgtggcgaa tggggcaacc ggtggcgcat actgtatatt tgcaaatctc actacacgtt
     4441 aaattaattg aataattatt tgtagattaa aaatatattt tgtaggtttg atgatgtttt
     4501 ttttacaatt actcatattt aagtaataaa taagtattat tgatatgttt tttaaattcg
     4561 acctctccct aaatccctct ccagggagag ggacttgccg agtgagaaaa tggcgtgata
     4621 ctcattaaaa gagctgctga gtcccctttc attaaataaa aagatccttt tcctttccct
     4681 tttggggaaa gtgcccgaag ggcgataggg gtctggagaa agggtcccga aacttcggga
     4741 gggatagagg tctatgcatt tttaattcat taaataatgc cagtttaaca aatatatcaa
     4801 taaataacac caactactta tatatttttc gaacctgaaa aacacctcac aatgataata
     4861 tcaaaaatat ttcaccgaaa tgaatggcgt atctgtatta atgtgggtta tgataatgaa
     4921 attgtggcta aaataaaaca actacccgat gctaaatgga gccgcacatt gaaagcctgg
     4981 catattccat ataccagaga ggcatttgat caattaaaaa cgttattccc cgatgtggaa
     5041 tatcagaaca catcaaccga acttgatact gagcag
//
LOCUS       DAOPZK010000137         5016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_21166_length_5016_cov_24.409796, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000137 DAOPZK010000000
VERSION     DAOPZK010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21166_length_5016_cov_24.409796"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1329
                     /locus_tag="PKH68_07740"
     CDS             178..1329
                     /locus_tag="PKH68_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12868.1"
                     /translation="MLRTLLFISPLYVTLFWLIVLNTNADKNSPPRAFLGKFMIFASV
                     VYFSHFLFFASLKELYVWVDAFYQYASLMVYPLYYIYFRLLTVDERFSFSKHARYLLP
                     STILFLFYVVGILLAPKTEYKMWVFQKNLPFSSVGIQWLSVVYVLIKAVFIIQVVLTV
                     TANSLLIRKYGYKAAQYYSNMHDTGTINVNLLNLSMILTAVASITIAILGRDFFENST
                     FRIGFPSVIFSALLFVIGWLGNRQKPINPAYEVNQEDKNELADLELNSGRKAGVLQKI
                     QELFEQKKVYLRSSLTIQEVADCIGTNRTYISSVINEFSKQNFSAFVNQYRLQELEKI
                     LRSGQNLQAHQLAELTGFGSVDSMKRALKMKTNKSLTEWKEEIIKQSHK"
     gene            1348..2190
                     /locus_tag="PKH68_07745"
     CDS             1348..2190
                     /locus_tag="PKH68_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HNX12869.1"
                     /translation="MRTKTYFLCCFLVVFSVSVFAAKQTKIVILHTNDTHSQVEPSES
                     NNMGGYARRMGEIDKIRSEESNVLLLDAGDFSQGSPYFNFFNGRIETQAMKMMRYDAV
                     TLGNHEFDNGMDTLAAVLKLVDLPIVNSNYGLENTPIAPFVKPYLILHRGGLKIGVFG
                     IGVNLKGLAFQKNYAGLELRDPLKAATQTSDFLKNEQKCDLVICLSHLGTSAEDSSPT
                     DYDLVAASRNIDVVIGGHSHKILENVTANNAAGKPVIIAQMGKSGLNLGRIDLMYEKQ
                     KAVK"
     gene            complement(2237..3181)
                     /locus_tag="PKH68_07750"
     CDS             complement(2237..3181)
                     /locus_tag="PKH68_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNX12870.1"
                     /translation="MSQGKHRLFTVLKWLITLAAYGFLVYKLARIEYWEELRKTMTGM
                     NLPRMMFLLAVLLLMPVNWMLESRKWQFLTSSTVAITFKTSLKAVLAGLNTGFITPNR
                     YGEFAGRIFFLPEAHRWTGVLLSFVNSVSQSLVVTFFGLIGAGVYFSRFYSPVNYTVW
                     LWVVIAVILICTILYLIFPGVVRNLISKKWFLKLRQAAIPIAALDRKSLLSFIFLSII
                     RYVVFCGQFYFMLKFFGIDIPATNALSAIPAMYLLVTYTPSFAASDPAIRGSYAVLIF
                     SVFSGDLSSIMLTGILIWLINFVVPMLVGSVIILRSKS"
     gene            complement(3168..3674)
                     /locus_tag="PKH68_07755"
     CDS             complement(3168..3674)
                     /locus_tag="PKH68_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNX12871.1"
                     /translation="MIRRILVLTGKFISDVIDYFYPPFRRYFGLQFFRYGVSGAANIV
                     FDWGLYSFIYNIVLQKQMLNLGIVTLSSPIAALFITFPITLFTGFLLQKYVTFTESEM
                     RGHKQLARYMVVVAINLLINYTGLKILNGVLGLWPLASKMIVTVVATFFSYFSQKKFT
                     FKIKNEPR"
     gene            3914..4702
                     /gene="rsmA"
                     /locus_tag="PKH68_07760"
     CDS             3914..4702
                     /gene="rsmA"
                     /locus_tag="PKH68_07760"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNX12872.1"
                     /translation="MTQQVRAKKYLGQHFLTDLEIARRIAESLPLSEALPVVEVGPGM
                     GVLTRFLLQNPLVDLTAVEIDRESVEYLHTHYPDLKLIEGDFLQMDLTSLFPGQFCVI
                     GNFPYNISSQIFFKVLEYRHQIPFAAGMLQKEVAERIASAPGKKAYGILSVLMQAYYT
                     VEYLFTVSEHVFDPPPKVKSAVIRFTRNEVEHLDCDEKLFRTVVKTAFNQRRKTMRNS
                     LKPLVPKESLMYADPIFDKRPEQLGVAEFVKLTNMVENALKENK"
     gene            4823..>5016
                     /locus_tag="PKH68_07765"
     CDS             4823..>5016
                     /locus_tag="PKH68_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HNX12873.1"
                     /translation="MSELRLFYHEDNKIKISKSLDILPKIELKNIIWIDLNDVALDVE
                     SRLEQFLKIYIQEDEEIEEI"
BASE COUNT         1579 a         1059 c         1046 g         1332 t
ORIGIN      
        1 caaatatata tgaataaatt catactctta ggtgcaaatc gtaaaaaaaa ccactcaaat
       61 cgtaaaaata tttgcttata tgattgatat tttctttttg taatcagaat taataagcat
      121 atatttgtgc aatgaattga ttttattttt tcttaaaaat agtcagctcc aaatccaatg
      181 ctcagaacct tattatttat aagcccgctt tatgttacac tgttctggtt gatagtgctg
      241 aacaccaatg cggataaaaa ctcacctcca agagcttttc tgggaaaatt catgattttt
      301 gcctctgtgg tgtatttttc ccattttctg ttttttgcct cactcaaaga gttgtatgtg
      361 tgggttgacg cattctacca gtatgcttca ctcatggttt acccgttata ttatatttat
      421 ttcagactac ttacagtcga tgagaggttt tctttcagta aacatgcacg ctatctgtta
      481 ccttcaacca ttttattcct cttttatgtt gtcgggattt tattggcacc gaagaccgag
      541 tataaaatgt gggtgtttca aaaaaatctt cccttttctt ctgttggaat ccagtggctg
      601 agtgtagttt acgttcttat aaaagcagtg tttatcattc aggtggtgct gacagtgaca
      661 gcaaactccc tgttgatacg aaaatacggt tacaaggcag cgcagtatta ttccaatatg
      721 catgatacag gcactatcaa cgtgaattta cttaatttat ccatgattct tacagccgtg
      781 gcatcaatta cgatcgccat actgggacgc gatttttttg agaacagcac cttccgaatt
      841 ggctttccgt cggttatttt ctccgcatta ctttttgtta tcgggtggct cggtaacagg
      901 caaaaaccaa tcaatccggc ttatgaagta aatcaggaag acaaaaacga gcttgctgac
      961 ttagaactca attccggacg taaagcgggt gtgttgcaaa agatccagga actctttgaa
     1021 cagaaaaaag tgtacctgag aagcagtctg acgatacagg aagttgccga ctgtatcgga
     1081 accaatcgaa cttatatttc atctgttata aacgaattca gtaaacagaa cttcagcgcc
     1141 tttgtaaacc agtacaggtt gcaggaactt gaaaagatac tgagatccgg tcaaaacctc
     1201 caggctcatc agctggccga acttaccgga tttggctcgg ttgactccat gaaacgggca
     1261 ttaaaaatga aaacaaacaa aagcctgacc gaatggaaag aggaaatcat taagcaatcc
     1321 cacaaataac agaaaaaaca catttttatg agaactaaaa cctattttct ttgctgtttt
     1381 ctggttgtat tttcggtatc tgtattcgct gcaaagcaaa ccaaaatagt gatactgcac
     1441 accaacgata ctcacagtca ggtagagccc tccgaaagca ataatatggg tggatatgcc
     1501 cgccggatgg gggagataga taaaatccgg tccgaagaat cgaatgtgct gctcctcgat
     1561 gccggtgatt tctcgcaggg aagtccgtat ttcaattttt tcaacggaag aattgaaacc
     1621 caagccatga aaatgatgcg ttacgatgct gtaacgctgg gcaatcacga gtttgacaat
     1681 gggatggata cactggctgc agtactcaaa ctggtagatt taccaatcgt taactccaat
     1741 tatgggctcg aaaatacccc cattgcaccg tttgtaaaac cgtatctgat acttcaccgg
     1801 ggaggtttga agattggtgt ttttggcatt ggagttaatc ttaagggatt ggcatttcag
     1861 aaaaattacg ccggtttgga actccgggat ccacttaaag cagccactca gacctccgat
     1921 tttttgaaaa atgaacagaa atgtgatttg gtaatttgtc tatcccacct gggcacctcg
     1981 gccgaagaca gttcacccac cgattacgat ctggttgctg cttcgcgaaa tattgatgta
     2041 gtgattggcg gacattcaca taaaatactt gaaaatgtta ccgccaataa tgcggccggt
     2101 aaaccggtca ttattgctca gatgggcaaa agcggactta atctggggag aatagacctg
     2161 atgtacgaaa agcaaaaagc agtaaaataa acctccccag agccagcccg gagactttgc
     2221 atgatttttt tttctgttac gactttgacc tgagtatgat taccgaaccc accaacatcg
     2281 gaacaacaaa attgatgagc cagatgagta ttccggtgag catgatggac gataaatccc
     2341 ctgaaaacac actaaaaata agtacggcat atgacccgcg tatggccgga tcggaagccg
     2401 caaaagaggg agtataagtt accagcagat acattgccgg gatggcactc agtgcatttg
     2461 tggcaggaat gtcaatacca aaaaacttca gcataaaata aaactgccca cagaaaacca
     2521 catagcgaat gatggaaagg aaaatgaacg atagtaaact tttccgatcg agtgctgcga
     2581 tggggatggc tgcctgccgg agtttgagga accatttttt tgaaatcaga tttcgtacca
     2641 ctcccgggaa tatcagatac agaattgtac aaatcaaaat aaccgcaata accacccaca
     2701 gccaaacggt ataattgacc ggtgaataaa aacgtgaaaa ataaacgccg gctccgataa
     2761 gaccgaaaaa ggtgacaacc aggctttgtg acacgctatt gacgaaactg agcaaaactc
     2821 ccgtccagcg atgggcttcg ggtaagaaaa aaatccgtcc ggcaaattca ccatagcggt
     2881 taggagtgat aaatcccgta ttcaatccgg ccaacacagc tttcaatgaa gttttaaagg
     2941 tgatagcaac ggtggatgaa gtgagaaatt gccatttccg gctttcgagc atccagttga
     3001 ccggcatgag cagcagtaca gccagtaaaa acatcatacg tggcaaattc atgcccgtca
     3061 tggtttttct gagctcctcc caatactcaa tacgggctaa tttgtaaacc aaaaagccgt
     3121 aggctgccag ggtaatcagc catttgagaa ctgtaaaaag cctgtgctta ccttggctca
     3181 ttttttatct tgaaagtaaa tttcttttgg gaaaaatagc tgaaaaaagt ggcgactacg
     3241 gtaacaatca ttttggaggc cagcggccaa agacccagca caccgttcag aatcttcaac
     3301 ccggtatagt tgataagcag attgatagcc actaccacca tgtagcgtgc gagctgcttg
     3361 tgaccccgca tttcggattc ggtaaaagtg acatatttct gaagcaaaaa accggtaaac
     3421 agggtgatgg gaaatgtgat gaaaagggct gcaatgggcg aactgagtgt aacaatgccc
     3481 aggttgagca tctgcttttg taatacaata ttataaatga acgaatacaa accccagtcg
     3541 aacacaatgt tggcagcacc cgatacaccg tagcgaaaaa actggagacc gaaatatctc
     3601 cggaaaggcg gatagaaata atcaatcacg tcactgatga atttgcctgt taaaacgaga
     3661 attctgcgaa tcattgtact caggatgagc aattgggttt aatgtagata attttgaaat
     3721 ggtaaaaaaa cgattattga cagaaatcag ccccaaaatt taggtttatc gaacaattat
     3781 ccttactttt gccgaaaata aacccgtatt tccatttgca aagatataaa ataaatggaa
     3841 atcaaccgga ttatattctt tttaatcaca tttcagaaaa gcctgtttat gcccgacgac
     3901 catcatcagc aatatgacac aacaagtacg agccaaaaaa tacttgggac agcatttcct
     3961 tacagacctg gaaattgccc gtcgtatagc ggagagcctg cctctcagcg aggctttgcc
     4021 tgttgttgaa gttgggcccg gcatgggtgt tctcacccgt tttttactcc aaaatcctct
     4081 cgttgattta actgccgtag aaattgaccg cgagtcggtc gaatatcttc acacgcatta
     4141 tcccgacctg aaactgatag aaggagattt cctccaaatg gatcttacct cactttttcc
     4201 cggtcagttc tgcgttatcg gcaattttcc ctataatatt tccagccaga tatttttcaa
     4261 ggtactcgaa tatcgccatc aaattccctt tgcagccgga atgcttcaga aagaagtggc
     4321 tgagcggatt gcctccgcac cgggcaaaaa agcctatgga attttgagcg tgctgatgca
     4381 ggcatactat acagtcgaat atcttttcac ggtgtcggag catgtctttg accctccgcc
     4441 gaaggtcaaa tcggctgtta ttcgctttac ccgaaacgaa gtagaacatc tcgattgcga
     4501 cgagaaactc ttccggacgg tggtgaaaac agccttcaac cagcgacgca agaccatgcg
     4561 caattcactg aaacccctgg tgcccaaaga aagcctcatg tatgccgatc ccattttcga
     4621 caaacgaccc gaacaactcg gagtggccga atttgtgaaa ctcaccaaca tggtggaaaa
     4681 tgcactgaaa gaaaataaat aataggtttt tttgtttttg gtgaatgcag gcaaggtctt
     4741 aactactcta attccaacaa tttaaatgtc tgagcagaaa tcccgtttct gatctgatac
     4801 caaaagaaag gagagaacaa tcatgtcaga attaagactg ttttaccacg aagataacaa
     4861 aataaaaata tcgaaaagcc tcgatattct ccccaaaatt gaactgaaaa atatcatctg
     4921 gattgacctc aacgatgtgg cactggatgt ggaaagccgc ctggaacaat ttctgaaaat
     4981 ctacattcag gaagacgaag aaattgaaga aatcga
//
LOCUS       DAOPZK010000138         4953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_21589_length_4953_cov_23.361780, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000138 DAOPZK010000000
VERSION     DAOPZK010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21589_length_4953_cov_23.361780"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1074
                     /gene="recA"
                     /locus_tag="PKH68_07770"
     CDS             52..1074
                     /gene="recA"
                     /locus_tag="PKH68_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNX12874.1"
                     /translation="MAKNIEVTDKPVVSEKLKALQLAMEKLDKDYGKGTIMRMGDSKV
                     EEVAVIPTGSVGLNIALGVGGYPRGRVIEIYGPESSGKTTLAIHAIAEAQKAGGIAAF
                     IDAEHAFDRYYAEKLGVNTNELLISQPDNGEQALEIADQLIRSAAVDIVVIDSVAALT
                     PKAELEGDMGDSRMGLQARLMSQALRKLTANISKTNTTCIFINQLREKIGVMFGNPET
                     TTGGNALKFYASVRLDIRRIGQLKDGEDATGNQVRVKVVKNKVAPPFRKAEFDIMFGE
                     GISRTGEIVDMGVEYNVIKKSGSWFSYGDSKLAQGRDATKNLIKDNPELAAELEAKIV
                     EAIKVK"
     gene            1465..4857
                     /locus_tag="PKH68_07775"
     CDS             1465..4857
                     /locus_tag="PKH68_07775"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNX12875.1"
                     /translation="MKKQFLFLFLLLPNFLFSQLIINELMTNNVSALMDESYNYSMWV
                     ELYNPSNTTAYNQINFYFTDDLTQPKKWRPLSKLINARSYSILWFESSDKSGHASFKL
                     EPGGGTLYMFNTNEQLVDLVVYPAQFRNTSYGRKTDGSNEWVFFEQHSPGTSNNGKKY
                     AAEQCSKPEFITPGGFYKNPITISLASPMTGDTIYYTRDGAEPTRSHTLYTPGSVIGI
                     NNTSIVRARCFSGNKLASDIVSNTYFINERNFKLPVVSIITEQKNLTDNTIGIYVQGT
                     NGITGNGMSTPANWNQDWWRPANFELYDTTLTARLNQELDIQIAGGWTRMNGQKTLKI
                     NPRNKFGKNMLNYDIFAATKPKMKYRSILFRNSGNDFDHSMMRDGYMQSLVMKRMNLD
                     YIAYEPAVCFMNGVYYGIQNLRERTDEDFIYSNYGYEEDEIYLLESWEMNSDVEYKKL
                     TNYITYNDITQKTVYDSVCKMIDVENYMDYFLSEIYLRNTDWPHNNMKVWKKKTGGKW
                     RWILYDTDFGYSIWDHDHTHNTLIWALGEQSGSDPANAPWSTILLRRLVLNETFKKRF
                     IDKFAIQISSTFELNRANAILDSLAAKIRTEIVYHKNKWGPGRDFNTDINMMKSFSAN
                     RPASMMNFIGARFLNNPGTAEIKISSNNPKATYKFNAETIQDDQINLKHFRNQTYTLE
                     ANPVPGFTFKRWEIPGNSASQMLIANGSTWKYSDGSAMPATNWFSKTYNDLSWKSGTA
                     PLGYGTLGHTTTIGYGSDANNKYPTAYFRKTVNITNLASKDNFIISTFVDDGAAVYVN
                     GTEVGRVNMPEGTLTFNTYSTTYNNGITGTFTVPKNLLAEGDNVIAVEVHQTNANSTD
                     LIFNLQFTCSISGESQVILSPVYSSTLINSGSIIAIYEKNSGSDPDADLNVVINEVVA
                     SNSLIADEYGQYDDYLEIFNNSNRAVNIGGWYLSDTPANPTLYQIPSTNASLTTIPAK
                     GRLVIRADGEPTQGVLHANFKLSKDGETVTLSRLNYLAELTQMDAVTFPYMEQNMSYS
                     RIPDGSDTWRIVPPTCNLTNVDWSATDDIRILARIYPTLVDNSLTIENAQNKLIRIID
                     LTGKVLMQQTCTDETKLLELSHLQPGVYILTAGNEKFRFIKK"
BASE COUNT         1576 a         1096 c         1057 g         1224 t
ORIGIN      
        1 tctgaattat ttttttatct ttgtattact aaaaatacaa aaaaataata tatggcaaaa
       61 aatattgaag taaccgataa accggttgta agcgagaaac tgaaagcatt gcaactggca
      121 atggaaaaac tggataaaga ctacggtaaa ggcaccatta tgcgcatggg cgacagcaaa
      181 gtggaagaag tggctgtgat accgaccggt tctgttggct taaatatagc gttgggtgta
      241 ggcggctatc cccgcggacg tgtgatagaa atttacggac cggaatcttc cggtaaaacc
      301 accctggcca ttcatgctat agccgaagct cagaaagcgg gtggaattgc cgctttcatt
      361 gacgcagaac atgcattcga ccgctactat gccgaaaagc tgggtgtaaa caccaacgaa
      421 ctgcttatat ctcagcccga taacggcgaa caggcactcg aaattgctga tcagctaatt
      481 cgttcggctg cggtggatat tgtagtgatt gactccgtag ctgcactcac tcccaaagcc
      541 gaactcgaag gggatatggg tgactccaga atgggacttc aggcccggtt gatgtcgcag
      601 gcattgcgta aactgaccgc caacatcagc aaaacaaata ctacctgtat atttatcaac
      661 cagttgcgcg agaaaatcgg cgtaatgttt ggcaatcccg aaacaacaac cggtggtaat
      721 gcccttaaat tctatgcttc cgttcgcctg gacatccgcc gcattggtca gttgaaagat
      781 ggtgaagatg ctacgggtaa tcaggtaagg gtgaaagtgg tgaaaaacaa agtggctcca
      841 ccattccgca aagctgaatt tgacatcatg tttggcgaag gaatttcgcg caccggcgaa
      901 atagtagaca tgggtgtgga atacaatgta atcaagaaaa gcggatcgtg gtttagctac
      961 ggcgacagca aactggctca gggacgcgat gccaccaaga acctgattaa agacaatccc
     1021 gaactggctg ctgaactgga agccaagata gtggaagcta tcaaagtaaa ataataacct
     1081 taaagaatac agactaatca catctgattc tgatttttat taagtacgag ttcttcttga
     1141 ctctttttat aaaaccataa tgtcgcacct acggagctta gaaacgacac attaatccat
     1201 tttctaccat aatatcgcac ctctggtgct aaaaatgaag cctcgtagag gcgatattat
     1261 ggtagaagat tattgaggat taaaattaaa gcctcgtaga ggcgacatta taaaaagtca
     1321 agacaacttc tatgtaatat attcaattac aaaaaagtaa taagggaatc catttccctt
     1381 attacttttt taaaccggtt tgatacttat cggaacgcat gttaatctat ctttttttcg
     1441 atttatttgt caataatttc taccatgaaa aaacagtttc tgttcttatt tcttctgtta
     1501 cccaatttcc tcttttccca attgattatc aatgagttga tgaccaataa tgtatcggct
     1561 ttgatggatg agagctacaa ttattccatg tgggtggaac tgtataatcc gagtaatact
     1621 accgcctaca atcagatcaa cttttacttc accgatgacc tcactcaacc caaaaaatgg
     1681 cgtccgttat caaaattaat caacgcccgc agttacagta ttttatggtt cgaaagcagc
     1741 gacaaaagcg gacatgccag tttcaaactc gaacccgggg gcggcacact ttacatgttc
     1801 aatactaatg aacaattagt tgatttggtg gtatatcctg ctcagttccg caatacctcc
     1861 tacggccgaa aaacagacgg aagtaatgag tgggtattct ttgagcaaca cagcccggga
     1921 acatccaaca acgggaaaaa atacgctgcc gaacagtgct caaaaccgga atttatcact
     1981 ccgggtggat tttacaaaaa cccgatcacc atctcactgg cttcgcccat gacaggcgat
     2041 accatctact acacccgaga tggtgcagaa ccaacccgca gtcataccct atacactccg
     2101 ggatcggtta ttggcatcaa taatacttca atagtccgtg cccgctgttt ttcaggaaat
     2161 aaactcgcaa gcgacattgt cagcaacacc tattttatca acgagcgcaa cttcaaattg
     2221 ccggtagttt ccattatcac cgaacagaaa aatctgaccg ataacaccat cggaatatac
     2281 gttcagggca ccaacggaat aaccggaaat ggcatgagta cgccggccaa ctggaatcag
     2341 gactggtggc gacctgccaa ttttgaactt tacgacacca cgctgacagc cagattaaat
     2401 caggaactcg acatacaaat agccggagga tggacacgta tgaacggaca aaaaacgctc
     2461 aagataaatc cgcgaaataa gtttggtaaa aatatgttga attacgacat ttttgcagcc
     2521 actaagccca aaatgaaata ccggagtatt ttgttccgaa attcaggcaa cgacttcgat
     2581 cattcgatga tgcgcgatgg atatatgcaa tcgcttgtga tgaagcgtat gaaccttgac
     2641 tatattgcct acgaaccggc agtttgcttt atgaatggcg tgtattacgg cattcagaat
     2701 ctacgcgaac gcaccgacga agattttatc tactccaatt acggatacga agaagatgaa
     2761 atttacctgc tcgaatcgtg ggaaatgaat tcggatgtgg agtacaaaaa gctgacgaat
     2821 tacataactt acaacgacat tacacaaaaa accgtttacg acagtgtctg taaaatgatc
     2881 gacgtggaaa attatatgga ttatttcctg agtgaaattt atctgagaaa tacagactgg
     2941 ccacacaaca acatgaaagt atggaagaaa aaaacaggtg gtaaatggcg ctggatactt
     3001 tacgatacgg atttcggcta tagtatatgg gatcatgatc acacacacaa cacattaatc
     3061 tgggcgttgg gcgaacaatc tggatcagat ccggccaatg caccctggtc aaccattctg
     3121 ctccgccgcc ttgtactcaa cgagaccttc aaaaagcgat ttattgataa atttgcaatt
     3181 caaatatcat cgacattcga acttaaccgt gccaatgcca ttctcgacag cctggcggcc
     3241 aaaatcagaa ctgaaattgt ttatcataaa aataaatggg gacccggacg cgacttcaac
     3301 accgatatca acatgatgaa gagtttttcg gccaacagac ccgcttcgat gatgaacttt
     3361 atcggggcac gctttctgaa caatcccggc acagccgaaa ttaaaataag ttcgaataat
     3421 ccgaaagcaa cctataaatt taatgctgaa actattcagg atgatcagat caatctgaaa
     3481 cattttagaa atcagactta tacgctggaa gcaaacccgg tgcccggatt tacgttcaaa
     3541 cgatgggaaa ttccgggaaa ttctgcttcg caaatgctga tagccaacgg aagcacgtgg
     3601 aaatattcgg atggcagtgc catgccggcc acaaactggt tttctaaaac atacaacgac
     3661 ctgagctgga aaagcggaac cgcaccgttg ggatatggca cgctggggca cacaaccact
     3721 atcgggtatg gcagcgatgc gaacaacaaa tatcccacag cctatttccg taaaacagtg
     3781 aatatcacaa atctggcatc gaaagacaat ttcatcatca gcacttttgt ggacgacgga
     3841 gcagccgttt atgtaaatgg aaccgaagtg gggcgcgtaa acatgccgga aggaacactt
     3901 acattcaaca catattcaac cacttacaac aacggtataa ccggcacctt tactgtaccc
     3961 aaaaacctac tggccgaagg agataacgtg atagccgtgg aagtacacca aaccaatgcc
     4021 aatagtacgg atttgatttt caatcttcag tttacctgca gtatttcggg agaatcgcag
     4081 gtaatcttgt cgccggttta ctcttccacg ctcatcaatt caggcagtat catagccatt
     4141 tatgagaaaa attcaggcag tgatccggat gcagacctga atgtggtgat caacgaagtg
     4201 gtggcttcca attcgctgat tgctgatgaa tacggacagt atgatgatta cctggaaatt
     4261 ttcaacaaca gcaaccgcgc agtaaatatc ggcggatggt atctgtcgga tacacctgcc
     4321 aatccgactt tgtatcaaat accttcgaca aatgcttccc tgacaaccat tccggctaaa
     4381 gggaggctgg taattcgtgc ggatggagaa cccacgcagg gagtattgca tgccaatttt
     4441 aaattgagta aggatggtga aacggtgacg ctttcgcgac tcaactacct ggccgaactc
     4501 acacaaatgg atgccgttac attcccgtac atggagcaaa acatgagcta ctcccgcata
     4561 cccgatggca gcgatacgtg gcggattgta cctcctacct gcaacctgac caacgttgac
     4621 tggtcggcaa ccgacgacat acgtatattg gcgcggattt atcctacgct ggttgacaac
     4681 agcctgacta ttgaaaatgc tcagaacaag ctgatacgga tcattgacct taccggaaaa
     4741 gtactgatgc agcaaacctg caccgatgaa acaaaactgc tggaattaag ccatttacag
     4801 ccgggtgtgt atatattgac agcaggaaat gagaaattca ggtttatcaa aaagtaaact
     4861 atgtgattca gtttccgttt attaaaataa aaaatccatt gcctgaccgg taatggattt
     4921 ttattttaga atatgctgtt ctaaaaacgc taa
//
LOCUS       DAOPZK010000139         4882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_22039_length_4882_cov_22.819764, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000139 DAOPZK010000000
VERSION     DAOPZK010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22039_length_4882_cov_22.819764"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1246
                     /locus_tag="PKH68_07780"
     CDS             74..1246
                     /locus_tag="PKH68_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNX12876.1"
                     /translation="MKTNLYFLLAMLLLFTGCKPTNPPDDPDDKPDDPPIVTVDSLGI
                     SISTIAFTAEKDASLIVVRTNRTWTAAKTADWLSLSAASGNKNTGFLIGAAPNDGLTR
                     ETSVTIKAGDKTKEITIIQAGTPKMTINVKGVPVNMILVEGREFTMGSSQQSSYGLPH
                     QVKLSDYYISETEVTNALWLAVKGSLPYTDHSEADKPALPVSEANWNVVTGDFMPALN
                     QLTGKTFRLPTEAEWEYAALGGKNTTGTAYAGSNTLEDVAWYLGNSGKQKQNVKGKDA
                     NQLGLYDMSGNVLEWCSDWYDYYYGFQIVNQSVVVPALQTNPKGPDSGTNKIVRGGSV
                     ESDLMWSYSPCHVKYRHSIIPTGIDGCWGNTGDPREPTCNFIKYTGFRLVISAQNQ"
     gene            1263..1880
                     /locus_tag="PKH68_07785"
     CDS             1263..1880
                     /locus_tag="PKH68_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX12877.1"
                     /translation="MKRTIIIAITVVSILFCPAQTVVKMKMPAQANEPLKVVVLFEEP
                     VPEGMPVVLGLMGYNITGGMAPYSYQWLQNGSLVGTGDVVIITPKKSDKFELKATDRN
                     RCYSTQSFSLKVIARIGSKDDENNSQYKIYPTLVKNDIINISLPEKEMLTQARIRIFD
                     TGGAMKYQTTITGSSTVMCHLPEGNYFVSVQTDEFHKVEKIIVQQ"
     gene            1896..>4882
                     /locus_tag="PKH68_07790"
     CDS             1896..>4882
                     /locus_tag="PKH68_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNX12878.1"
                     /translation="MNIRLINQTILLSLFLVLSAVGSANAASPNALGAKLFAENFFKT
                     KSPQFAPGKVIQPPVLKQCYQSASLKQTPVFVFQHEQSGFVVLGQSTTGFQILGYSDK
                     ANFNPTQIPESLQQLLRLYENANPDSIVGIQSTKGTTVVVGPLLDEAGVGLNQFHHEN
                     VGDSWTGCVATAMTQIMCYYRYPDKGIGSHCFTNSSYGQLCADFENTRYNWTNPTDED
                     FKLLSYHVGVSMDMNYNGSPLGSSPLSSSYPYALQEYFGYTCEPFNEFSRNSPVIQNV
                     IAQRKPIYVELPGNPGHAVVLDGYDSNGFFHVNFGWGGGSNGYYLLNSNSLIYMNPYK
                     FGTNLVSALLIQPGHVPLNKTDSLALVSIKNNISNLDWDLTNTQKRTGVTTLNGRVIG
                     LQVYSYNTNGNEGSIPEDIGNLSELISLNVTGKLHGTIPNSITNLTKLQSLSISNGSG
                     TLSDTIPAEIGNLTNLTNLSIFNAAKGPIPKSIGKLTNLAYLWLSTGNIDGAIPEEFY
                     SLKNLQTITLNDQKLSGTISEKIGNLKNVGFLDIANNNLSGKIPTTIGEMNNLLYLDL
                     SGNKLSGEIPQSLNNCTSISAFKLNNNELEGEVPAIFDNKQKLTRLDLSNNKLTGISE
                     NIGKLTNLESLNFDNNRLTSLPNSLNKLRALQSLSANSNKINRLSESMNQLTSLNTLN
                     LAYNELTTFHPDICLLPNLASINLSNNKITRLPGLIAEINTPILQIENNELSGPIPAR
                     LLQTEPGYYRFQNNRFVYNDLPSGTNFTNRIGSQKPLKLLKNTFKGNLGDSIEIDIRK
                     ICSNLNKHDTYVWCEHPKEKKELQPAIKIEDGPVLHLKLSDKNIGKKYYCLITNDSAA
                     TYKDPQIYYRIPCLESLITDTVYLAIQTEEEFLAEKYPEQYIVKSESLQKKELSDKSV
                     TLISPFKMRGVKKWEGSADGKTWYELSTTMTQNDLKTNLISVKEEELKISPKTPAYYR
                     CALLETNCEPAYSDTMKINPYGKV"
BASE COUNT         1594 a         1062 c         1003 g         1223 t
ORIGIN      
        1 ataaattatc tgaaaattaa ctgatttttc agaattccat tcaattattt gcaatccaag
       61 atatccttat gctatgaaaa caaaccttta ttttctcctg gcaatgctcc tgctttttac
      121 aggctgcaaa cccacaaatc cacccgatga cccggatgat aaaccggatg acccacctat
      181 tgtaacggtc gattcgttgg ggatttcaat cagcactata gcatttacag ccgaaaagga
      241 tgcaagttta atcgtagtaa gaaccaaccg cacctggact gccgccaaaa cggccgactg
      301 gctgtcgctt tcggctgcat cgggcaacaa gaataccgga tttctgattg gtgctgcacc
      361 aaacgacgga ttgacgcgcg aaacatcggt tacaatcaaa gcgggtgaca aaaccaaaga
      421 aataacaata atacaggcag gtactccaaa aatgaccata aacgtgaaag gagttcctgt
      481 aaacatgata ctggtagaag ggcgcgagtt cacgatggga agcagccagc agagcagcta
      541 cgggctgcca catcaggtca aactgagtga ttattacata tccgaaactg aggtgaccaa
      601 cgccctgtgg cttgccgtga agggttcgct gccctacacc gaccacagcg aagccgataa
      661 acctgcacta cctgtgagcg aagcaaactg gaatgttgta accggcgatt tcatgccggc
      721 actcaatcaa ctgaccggca aaacattccg tttgcccacc gaagccgaat gggaatatgc
      781 ggccctgggt ggtaaaaaca ccactggcac cgcatatgca ggcagtaaca cactggaaga
      841 cgtagcctgg tatctgggca actccggtaa acaaaaacaa aatgtaaaag gaaaagatgc
      901 caatcagctc ggtctgtatg acatgagcgg caatgtgttg gaatggtgca gcgactggta
      961 cgattattat tacggtttcc agatagtgaa ccagtcagta gtggttcccg ctctgcaaac
     1021 caacccgaaa ggtcccgata gtggaaccaa taaaattgtg cgcggcggaa gtgtggaaag
     1081 tgacctcatg tggagttatt cgccctgcca cgtgaaatac cgtcactcca tcattcctac
     1141 gggtatagat ggctgctggg gcaacacggg agatccgcgg gaacctacgt gtaattttat
     1201 aaaatatacc ggtttcagac ttgttatttc tgctcaaaat caataaaaac atactttctg
     1261 tcatgaaaag aactatcata attgcaatta cagttgtcag catattattt tgccctgcgc
     1321 agacggtagt aaaaatgaaa atgcctgcac aggctaacga accgctcaaa gtagtggtgc
     1381 tttttgaaga accagtgccc gaaggtatgc ccgtagtgct gggcttgatg ggatataata
     1441 tcaccggcgg aatggcaccc tacagttatc agtggttaca aaacggatct ttagtcggta
     1501 cgggtgatgt ggttattatc accccaaaaa aaagcgataa atttgaactg aaggccactg
     1561 acagaaatcg ttgctactcc acccaatcat tttcattgaa ggtaatagcc agaattggca
     1621 gtaaagacga tgaaaataac agtcaataca aaatctaccc aacccttgta aaaaacgata
     1681 ttataaacat ctccttacct gaaaaagaaa tgctgacgca agccaggata cgaatttttg
     1741 atacaggcgg ggcgatgaaa tatcaaacta caattaccgg aagttccact gtaatgtgtc
     1801 atttacctga aggaaattat tttgtttcgg tacaaaccga tgaatttcat aaagttgaaa
     1861 aaatcatcgt tcaacaataa ataagatcta caactatgaa tatacgatta atcaatcaaa
     1921 cgatactcct gtctttgttt ttggttttat cagctgtagg ttcagcaaat gcagcatcgc
     1981 ccaatgcttt gggagcaaaa ttatttgcgg agaatttttt caaaacaaaa agccctcagt
     2041 ttgcacccgg aaaagtcatt cagccaccgg tattgaagca gtgctatcag tcggcaagcc
     2101 tgaagcagac accggtattt gtatttcagc acgagcaaag cggttttgtg gtattaggac
     2161 aaagcacaac gggatttcag attttgggat attccgataa agccaatttt aatccaactc
     2221 aaattcctga aagcttacaa caactgcttc gactttacga aaatgccaat cccgacagca
     2281 tcgtgggaat acaaagcacc aaaggaacaa ccgtagttgt gggtcctttg ttagatgaag
     2341 ccggagtagg tctcaaccag tttcatcacg aaaacgtggg agacagctgg acgggctgtg
     2401 tggctacggc catgacacaa atcatgtgtt attacaggta tcccgacaaa gggattggtt
     2461 ctcattgctt tacaaattcg tcttacgggc aactttgtgc cgattttgaa aacactcgct
     2521 acaactggac caatccgact gacgaagatt tcaagttatt gtcctatcac gtaggagtat
     2581 ccatggacat gaattataat ggcagtccgt taggcagttc acccttatct tcaagctatc
     2641 catatgcttt gcaggaatat tttggatata cgtgtgagcc attcaatgaa ttctcacgca
     2701 attcaccggt tattcaaaac gttatagctc agcgaaaacc catttatgtg gagttgccgg
     2761 gcaatcctgg acatgctgta gtattggacg gatacgacag caacggattt ttccacgtca
     2821 acttcggttg gggaggtggc agcaatggtt actatctgct caatagcaat tcattgattt
     2881 atatgaatcc ttacaaattc ggaaccaatt tggtcagtgc gcttttaatt caacccggcc
     2941 atgtacctct gaataagacc gattcacttg ctttagtttc aataaaaaac aacatcagta
     3001 atttagactg ggatttaact aatactcaga aaagaacagg cgtaactacg cttaacggca
     3061 gggtaattgg gctacaggtt tactcatata atacgaatgg gaatgaaggc tcgatacctg
     3121 aagatatcgg aaacctgagt gagctaatca gcttaaatgt tacgggaaaa ttacacggta
     3181 caattccaaa ttctatcacc aaccttacca aactccagtc cctaagcatt tctaatggtt
     3241 caggcacact aagtgatacc ataccggcag aaatcggaaa tcttacaaat ctcacaaatc
     3301 tcagcatttt taatgcagcc aaaggcccta tccctaagtc cattggcaaa cttacaaatc
     3361 tggcatatct ttggttgtcc accggaaata tcgacggagc gatacccgaa gaattttact
     3421 ctttgaaaaa tttgcagact attacgctca atgatcagaa actgagcgga accatttcgg
     3481 aaaaaatagg aaatctaaaa aatgtgggtt ttttagatat tgccaataac aatctttcag
     3541 gtaaaatacc taccacaatt ggcgaaatga ataatctgct ttatctggac ttgtccggca
     3601 acaaattatc cggtgaaatt cctcaatcgc tgaataattg cacttcgata tcagcattta
     3661 aacttaacaa caatgaactg gaaggtgagg tacccgctat ttttgacaac aaacaaaaac
     3721 tgacccgact tgatctgagc aacaataaac tgacgggcat atccgaaaac ataggcaaac
     3781 tcacaaatct ggagtcatta aactttgaca ataaccgcct cacttcgctc ccaaattcgc
     3841 tgaataagtt gcgggcattg caatcattgt cggctaattc caataaaatc aaccgcctat
     3901 cggagagcat gaaccaactg acaagtttga acaccttaaa cctggcatac aacgaattaa
     3961 ccacttttca tccggacata tgtttactgc ccaatttagc atcgattaat ctcagcaata
     4021 ataaaatcac ccgtttgccg ggcttgatag ctgaaataaa tacgccaatt cttcagattg
     4081 agaataacga actttcggga ccgataccgg ccaggttatt acaaaccgag ccgggttatt
     4141 accgctttca gaataaccgg tttgtgtaca acgacctacc atcgggaact aatttcacga
     4201 acagaattgg atctcaaaaa ccgctgaaac tcctgaaaaa cacgttcaag ggcaatctgg
     4261 gtgattccat tgaaattgat attcgaaaga tatgcagtaa tttgaataaa catgacacgt
     4321 atgtatggtg cgaacatccg aaagaaaaga aagaactaca acctgcaata aagattgaag
     4381 atggcccggt attgcacctg aaattatctg ataaaaatat cggaaaaaag tattattgct
     4441 taataacaaa cgacagtgca gctacttaca aagaccctca gatatactac cgcattccat
     4501 gccttgagag cttaattacc gatactgtat atttggctat acaaactgaa gaagagtttc
     4561 tcgctgagaa atatcccgaa cagtacattg taaaatcgga atcgttgcag aaaaaagaac
     4621 tgagcgataa atcggttacg cttatttcgc ctttcaaaat gcgcggagtg aagaaatggg
     4681 aaggctcggc cgacggcaaa acgtggtacg aactttcgac caccatgaca caaaacgatc
     4741 tgaaaaccaa tctgatttcg gtaaaggaag aagaattaaa aatatcgcct aaaacacctg
     4801 cttattaccg gtgtgcattg ctggaaacaa actgcgaacc ggcttacagc gatacgatga
     4861 aaattaatcc gtatggaaaa gt
//
LOCUS       DAOPZK010000140         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_22136_length_4868_cov_28.497611, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000140 DAOPZK010000000
VERSION     DAOPZK010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22136_length_4868_cov_28.497611"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PKH68_07795"
     CDS             complement(<1..1154)
                     /locus_tag="PKH68_07795"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNX12879.1"
                     /translation="MKILVLNCGSSSVKYKLLDMTSKEVLGSGGVEKIGMKGSFLKHT
                     KQNGEKVILEGEVLEHQLAIEYILGVLTSEKHGCIKNMEEIDAVGHRVVHGGEKFNSS
                     VVITDEVIRNIEACIEIAPLHNPPNLAGIFAISDLLPQVPQVAVFDTAFHQTMPEHAY
                     MYGIPYSLYKKYGIRRYGFHGTSHRYVSRRACEFLGLDYNKTKIITAHIGNGGSITAI
                     ENGKSVDTSMGFTPVEGLMMGTRSGDVDLGVVTYLMEKEMISTSATTTLFNKHSGVLG
                     VSGISSDMRDIEKAISEGNQRAKLALGMYEYRIKKYIGSYAAVLNGFDVLVFTGGVGE
                     NQTITRERICNGLSYLGIEIDPEINRIRGEELLISTSNSRVKVVVIPTDE"
     gene            complement(1171..2127)
                     /locus_tag="PKH68_07800"
     CDS             complement(1171..2127)
                     /locus_tag="PKH68_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNX12880.1"
                     /translation="MSEPIREPIERYGLSKRNQKKTLFSRIGVVGCGKDGSVIATAAA
                     SHGMEVIFLEPSEERIANAYARIETKLDRQIKGWGLTENEKKAIMGRISGTMDYEDFH
                     GCDFVIEAIRYNDMTGERRVEQRKEVFRHLEKVLSPQAIIASNVTTVIVSDLASDLEN
                     KSRCIGLHFLSNVPDSQMIEIVRGLETSDATYEKVCQFARLINYQYVPVVESAGLVSI
                     RLFLIQLNEACSILMENISNTEDIDRIMTVGFGHRQGLFRIADRLGIEKIVKLLENLY
                     DEYGHVKYKTSPILLRMYRAKHFGVSTKKGFYTYDDMGNIIK"
     gene            complement(2216..3217)
                     /gene="pta"
                     /locus_tag="PKH68_07805"
     CDS             complement(2216..3217)
                     /gene="pta"
                     /locus_tag="PKH68_07805"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNX12881.1"
                     /translation="MKLLEDIMQRAKDNLQRIVLPEGTELRTLKAADFILKEKAAKLI
                     LIGNEIEIKQLAADNNLTNISEAQLIDPETYPDMEKYANLLYEIRKSKGMTYDEAAAL
                     AKDPLYLACLLIKNGDADGELAGARNTTGNVLRPAFQIVKTKPGIKIVSGALLMFTPK
                     PEYGENGLLIFADCAVNPNPTADELAQIAVSTADTAKALGGFEPRVAMLSFSTKGSAQ
                     HELVDKVVEATRIAKEMAPDLQIDGEFQADAAIVPSVGQSKAPGSAIAGKANVLVFPD
                     LQAGNISYKLVERFSGAQAIGPVLQGIAAPINDLSRGCSVEDIIRMITITANLAMKK"
     gene            3507..4430
                     /locus_tag="PKH68_07810"
     CDS             3507..4430
                     /locus_tag="PKH68_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX12882.1"
                     /translation="MKSNILLSLCLLISLTLVSQTVPQASTGTIRHFEQFPSKYVDAR
                     NVDVWLPQNYNPKKKYAVLYMHDGQMVFDKTITWNHTAWEVDEVFGKLMDEKKIRDCI
                     VVGVWNVDGKRSADYYPQKVLDLMPEPTRSEVLEMVGNPPCADNYLKFLVTELKPFID
                     KNFSTHRNPKNTFVMGSSLGGLISAYALCEYPNIFGGAGCLSIHTPMIDLDKLNANNP
                     DEDNPGAQAFREYLMKNLPKANSRKIYFDYGNKTIDVYYAPYQHRVDEVMKKKGYNNQ
                     YWMTRFYEGDDHSEIAWSRRLHVPAVFLLGN"
     gene            4576..>4868
                     /locus_tag="PKH68_07815"
     CDS             4576..>4868
                     /locus_tag="PKH68_07815"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNX12883.1"
                     /translation="MKDHRIDQYIDQSTETLQPVIIHIRELMHRAVPEIVETIKWNSP
                     CFMVNGKIICVIQAFKKHVNFVFHKAPETPDTDTFLVDIGERSKMKGLKQLTQV"
BASE COUNT         1306 a         1078 c         1012 g         1472 t
ORIGIN      
        1 tcttcgtcgg tcgggataac aaccacttta acccgagaat tagatgtgct tatcagcagt
       61 tcttccccgc gtatcctgtt gatttccgga tcaatttcaa tacccaaata actcagtccg
      121 ttgcagattc gctcacgcgt aatggtttga ttttcaccta ctccacccgt aaatacaagt
      181 acatcaaatc cgttgagtac tgccgcatag gaaccaatat attttttaat ccgatattcg
      241 tacataccca gagccagttt tgcccgttga tttccttcgg atattgcttt ttcgatatcg
      301 cgcatatccg acgaaattcc cgaaacaccc agcacgccgc tgtgtttatt gaacagggtg
      361 gtagttgcgc tggtactgat catctctttt tccatcaggt aagtcaccac gcccaaatct
      421 acatcacccg aacgggttcc catcatcaga ccttctacgg gtgtaaatcc catggaggta
      481 tccaccgatt ttccgttttc gatggccgta atagagcctc cgttgccaat atgagctgta
      541 atgattttgg ttttattgta gtccagaccc aaaaactcac atgccctgcg cgatacatag
      601 cggtgactgg tgccatgaaa accgtaccgg cgaataccgt attttttata cagagaataa
      661 ggaattccgt acatataggc atgttcgggc atggtctgat gaaaagccgt atcgaaaacc
      721 gccacctgag gaacctgcgg cagtaagtcg ctgatagcaa aaatacctgc cagattcggc
      781 ggattgtgaa ggggagctat ttctatacag gcttcaatat tccgtatcac ttcgtcggta
      841 atcaccacac tggagttgaa tttttcgcct ccatgcacta cacgatgccc tacggcatca
      901 atttcttcca tgtttttgat gcagccatgt ttttcgctgg tgagtacacc cagaatatat
      961 tctatagcca gttgatgttc aaggacttca ccttcaagaa ttactttttc tccgttttgt
     1021 ttggtatgct taagaaatga tcctttcata cctatttttt ccacccctcc cgatccaagc
     1081 acttctttgc ttgtcatatc caacagttta tacttcaccg aagaactgcc gcaattcaat
     1141 accaatattt tcataactta ctttcctcca ttattttata atatttccca tgtcgtcgta
     1201 ggtgtaaaac ccttttttgg tacttacccc gaaatgttta gcacgataca tacgtaataa
     1261 tatcggtgaa gttttgtatt tcacatgtcc atactcatcg tacaggtttt caagcagttt
     1321 tacgattttc tctattccaa ggcggtcggc tatacgaaat aatccctgtc ggtgaccaaa
     1381 tccaacagtc atgatgcggt caatatcttc ggtatttgaa atattttcca tcagaatgga
     1441 acaggcttcg ttgagttgaa tcaggaaaag acggatgctg accaatccgg ccgattcgac
     1501 aacaggaaca tactgatagt tgataaggcg ggcaaactga cagacttttt cataggttgc
     1561 atccgatgtt tcgagaccgc gtactatttc tatcatttgc gagtctggca cattcgagag
     1621 gaaatgcagt ccgatacagc ggcttttatt ctcaaggtct gaagccaggt ccgaaacaat
     1681 tacagtagta acattggagg cgatgattgc ctgtggcgac agcacttttt ccagatgacg
     1741 gaatacctct ttgcgttgct ccactcttcg ttctccggtc atatcgttgt agcgtatggc
     1801 ttcaatcaca aaatcacatc cgtgaaagtc ttcataatcc attgtgccgc ttatgcgacc
     1861 catgatggct ttcttttcat tctcggttag tccccatcct tttatctgcc ggtcaagttt
     1921 ggtctctatt ctcgcgtaag cattagctat tctttcttcg gagggctcca ggaaaatgac
     1981 ttccatccca tgcgatgcgg ctgcagtggc tattacgctg ccgtccttgc cacatcccac
     2041 cacgccaatc ctcgaaaaca gggttttctt ctgattgcgt ttgctcaacc catatctttc
     2101 aataggctct ctaattggtt ctgacattct tgttggtttt attatttttt gaaaaaattc
     2161 aaatgtacaa ttatatttaa aaatccaaat ttagtaagtg tggattttca gaatactatt
     2221 ttttcattgc cagattggcg gtaattgtaa tcattcggat aatatcttcc accgagcatc
     2281 cgcgcgacag gtcgttgatg ggtgctgcaa ttccctgcag tacagggccg atagcctgag
     2341 ctcctgaaaa ccgttccacc agtttgtagc tgatgtttcc ggcttgcaaa tcggggaata
     2401 caagtacatt ggctttaccc gctatggcac ttcccggagc tttgctctga cctacggagg
     2461 gaacaatggc tgcatcggcc tgaaattctc cgtcaatttg cagatcgggt gccatttctt
     2521 tggctatgcg ggtagcttcc accactttat ccaccagttc atgctgtgcg ctgcctttag
     2581 tcgaaaaact caacatggct acacgcggtt cgaaaccacc cagcgctttg gctgtatcgg
     2641 ctgtactcac agctatttgt gccagttcat cagctgttgg gttggggttt acagcgcagt
     2701 cggcaaatat gagcagcccg ttttcaccgt attcgggttt gggggtgaac atgagtagtg
     2761 cgccggatac aatttttatc ccgggttttg ttttgactat ctgaaaagcc ggtcgaagca
     2821 cattgcctgt tgtgttgcgt gctcctgcaa gttctccgtc ggcatcgccg ttttttatca
     2881 acagacaggc cagatagagt gggtctttag caagtgcagc tgcttcgtcg taggtcatcc
     2941 ctttcgattt tcggatttca tacagcaggt tggcgtattt ttccatatcg gggtaagttt
     3001 ccggatcaat gagttgagct tcgctgatat ttgtcaggtt attgtcagca gctaattgct
     3061 tgatttcaat ttcattgccg ataagtatga gttttgctgc tttttctttc agaataaaat
     3121 cggcagcttt cagtgtgcgc agttctgttc cctcgggcag tacaattcgc tgtaaattgt
     3181 ccttagcccg ctgcatgata tcttctaata gtttcatggc tgaatattta agtttataat
     3241 aagttggtgt atggaagttt gaatatatct gaaattcagt tgaatatgct gagtgtcagg
     3301 taaggaaatc ctgacaattg cataattaca aaagtactat gtttttatta aattgcaatg
     3361 tagagattgc aattttttga aattattgtg cattgttata cgcaatatta gttgtatttt
     3421 tggcgtcgta gttattttgc gtattaacaa aaaagaacca aaggaaaatg gttctgctca
     3481 gcataaatcc ggtaattttt ttatccatga aatcaaatat tcttttaagt ttatgtttac
     3541 ttattagcct gacattggtt tcacaaaccg ttccgcaggc aagtacaggt actattcgac
     3601 attttgaaca attcccatcg aagtatgttg atgcccgaaa tgtagatgtt tggcttccac
     3661 agaattacaa tcctaaaaag aaatatgccg tactctatat gcacgacgga cagatggttt
     3721 tcgacaaaac gattacctgg aaccataccg cctgggaagt ggacgaggtc ttcggaaagc
     3781 tgatggatga aaagaaaata cgcgattgta ttgtggtagg agtttggaat gtggacggca
     3841 agcgttcagc cgattactat ccgcaaaaag tactcgacct tatgccggaa ccgacccgca
     3901 gcgaggtact ggaaatggtc ggaaatcctc cctgtgcaga taattacctg aaatttctgg
     3961 tcacagagct gaagccattt attgacaaaa atttctctac tcacagaaat cccaaaaaca
     4021 cctttgtgat gggaagcagc cttggcggac tgatttctgc gtatgcgctg tgtgaatatc
     4081 ccaacatttt cgggggagca ggctgccttt ccattcatac gccgatgatt gatctggata
     4141 aacttaatgc caataatcct gatgaagaca atccgggtgc tcaggctttc agagaatatc
     4201 tgatgaaaaa tctccccaaa gccaattccc gaaaaatata ttttgactat ggaaataaaa
     4261 caatcgacgt ttattacgca ccataccagc accgggtgga tgaggtgatg aagaaaaaag
     4321 ggtacaacaa ccaatactgg atgacccgat tttacgaggg cgatgatcac tcggaaattg
     4381 cctggtcacg aaggttgcat gttcccgctg tatttttatt gggcaactaa aaaattatta
     4441 taattgtgaa acacacatgt ccaagtaaaa tatttttgac acacacgggt atgattaaaa
     4501 aactcacaac aaatgataga cgctcaatat tcccccttta gggggttagg gggcagatat
     4561 ataaattata aacagatgaa agaccacaga atagatcaat acatcgacca atcgacagaa
     4621 acattgcaac cggtcattat ccatatccgc gaacttatgc accgggcagt gcccgaaatt
     4681 gttgaaacca tcaaatggaa cagtccctgt tttatggtca acggtaaaat catttgtgtg
     4741 atacaggctt tcaaaaaaca cgtcaatttt gtattccaca aagctcccga aacacccgat
     4801 acagatactt ttttggttga tattggtgaa agatcgaaaa tgaaagggtt gaagcaattg
     4861 acacaggt
//
LOCUS       DAOPZK010000141         4859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_22207_length_4859_cov_24.198585, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000141 DAOPZK010000000
VERSION     DAOPZK010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22207_length_4859_cov_24.198585"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1403)
                     /gene="sufD"
                     /locus_tag="PKH68_07820"
     CDS             complement(3..1403)
                     /gene="sufD"
                     /locus_tag="PKH68_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNX12884.1"
                     /translation="MNNLDKNNNISSISSPSGGLGEAGYIDLYTQHHETIKKHSADVI
                     NHKRDEAFEKFRELGFPTSRLENYKYTDLRDALAVDYGLNFSRIRFAVDPYNVFKCDV
                     PGISSYLYFVVNDAFYPVNIERNNSLPEGVIIGSLKKAAEEHPELVKKYFGKLSEKQK
                     DGLVAFNGAFAQDGFFMYVPKNVVLDKPVQLINIMRSSVDFMANSHNLIILDEGAGAQ
                     LLVCDHTSDDVRFLCNRVTEVFVGENASYEHYKLENVHNKTTNLCTLLVDQDTSSRFL
                     GNVITLHNGLTRNNIEIDLDGEHCETLMCGMALADKNQQIDNFTSVIHNKPNSHSTEL
                     FKYVLDDSAKGGFTGKLYVAKDAQKTQAYQTNRNILLKASAKMRTKPQLEIYADDVKC
                     SHGATIGQLDDKAMFYMRQRGIPESEARLLLMYAFTADVIENIRIDALQDRIKMLVEK
                     RLRGELSKCEGCVICK"
     gene            complement(1405..2277)
                     /locus_tag="PKH68_07825"
     CDS             complement(1405..2277)
                     /locus_tag="PKH68_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNX12885.1"
                     /translation="MTETSIKLFESRKIRTHWDETEEQWYFSVIDVVEVLTESTIPKR
                     YWSDLKKKLTSEGSEVYEKIVRLKMIADDGKNRETDAADTKTLLRIIQSIPSPKAEPF
                     KQWLAKVGYERIQEIQDPSQSIDRARENWKRSGRSEKWIQQRMTGQETRNKLTDYWKD
                     AGIKEQDEFAYLTNIIHQEWSDLTVKQHKQLKGLKNQNLRDHMSEAELIFTALAELST
                     RQIAQTEQATGLKENAVAGKKGGKIAKNARKELELQTGRNVITEENFLPPAKEKEELE
                     AHLNPPKERSKKDL"
     gene            complement(2343..>3083)
                     /gene="sufC"
                     /locus_tag="PKH68_07830"
     CDS             complement(2343..>3083)
                     /gene="sufC"
                     /locus_tag="PKH68_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNX12886.1"
                     /translation="FMLQIKNLHANVNGKEILKGLNLTVNAGEIHAIMGPNGAGKSTL
                     ASVLTGNPAFEVTEGKVIFEGNDLFELNPEQRASAGIFLSFQYPVEIPGVSMVNFMRA
                     AINEQRKYRGQEPIAATDFLRLMREKKELVEMDNKLTNRSVNEGFSGGEKKRNEIFQM
                     AMLEPKLAILDETDSGLDIDALRIVANGVNKLKRPDNASIVITHYQRLLDYIIPDFVH
                     VLYDGKIVKTGDKNLALELEEKGYDWLK"
     assembly_gap    3084..3183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3184..4619)
                     /gene="sufB"
                     /locus_tag="PKH68_07835"
     CDS             complement(<3184..4619)
                     /gene="sufB"
                     /locus_tag="PKH68_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNX12887.1"
                     /translation="MSDDILDEITQSDYKYGFTTDIETDVIPMGLNEDVIRLISAKKN
                     EPEWLLDFRLKSYRHWLTMQMPTWAHLTIPEIDYQNMSYFAAPRKNAPKSLDEVDPEL
                     LNTFNKLGIPLDEQKVLSGMAVDAVMDSISVKTTFRENLAEMGIIFCSFSEAVEHHPD
                     LVQKYMASVVPYTDNFFATLNSAVFSDGSFVYIPKGVRCPMELSTYFRINAMNTGQFE
                     RTLIVADEDSYVSYLEGCTAPMRDENQLHAAIVEIVVMKNAEVKYSTVQNWYPGDKEG
                     KGGIYNFVTKRGICKGENSKLSWTQVETGSAITWKYPSCILLGDNSSAEFYSVAVTNH
                     HQQADTGTKMMHIGKNTSSHILSKGISAGFSNNSYRGLVRVNPKAENARNYSQCDSLL
                     LGDKCGAHTFPYMDINNDTAIVEHEATTSKISEDQIFYCNQRGINTETAVGLIVNGYA
                     KEVLNKLPMEFAVEAQKLLQITLEGSVG"
BASE COUNT         1154 a         1109 c          967 g         1529 t
ORIGIN      
        1 tttcatttac agatcacgca cccttcacat ttactcagtt cgccgcgtaa tcgtttttca
       61 acgagcattt ttattctgtc ctgcaacgca tctatccgga tattttcaat cacatcggca
      121 gtgaaggcat acatgagcag caaccgcgct tcactctccg gaataccccg ctgccgcata
      181 taaaacatgg ctttgtcatc cagttgtccg atagtggctc catgcgaaca tttcacgtca
      241 tcggcataga tttcaagctg gggtttggtg cgcatcttgg cagaagcttt caataagata
      301 tttctgttgg tctggtaggc ttgtgtcttt tgcgcatcct ttgccacata gagttttcct
      361 gtaaaccctc ccttggcaga atcatccagc acatatttaa agagttcggt actgtggctg
      421 ttcggtttat tgtgtatgac tgaagtaaag ttatcaatct gttgattttt atcggctaat
      481 gccataccac acataagggt ttcgcaatgt tcaccgtcta aatcaatttc gatgttattg
      541 cgggttagtc cgttatgcag cgttatcaca ttgcccaaaa aacggcttga agtatcctga
      601 tcaaccagca acgtacagag attggtagtt ttgttgtgca cattttcgag tttataatgc
      661 tcatacgatg cattttcgcc aacaaaaact tctgtcaccc gattgcaaag aaaacgtaca
      721 tcgtcagagg tgtggtcaca taccagcaac tgtgcacctg caccctcatc aagaataatc
      781 aggttgtggc tgttggccat aaaatcgacg cttgagcgca tgatattaat cagttgcacc
      841 ggtttgtcga gtactacgtt tttgggaacg tacataaaaa acccatcctg tgcaaatgca
      901 ccgttgaagg caaccagtcc atccttttgt ttttcagata attttccgaa atacttcttt
      961 accagttcgg gatgttcttc ggcggccttt ttcagacttc cgatgattac cccttcgggc
     1021 aaagaattgt ttctttcaat attgacggga taaaaagcat cattgaccac aaaatacaaa
     1081 taggaagaaa tgcccggcac atcgcacttg aacacattgt agggatcgac ggcaaagcgg
     1141 atacggctga aattcagtcc atagtccaca gcaagggcat cccggagatc ggtatattta
     1201 tagttttcga gccgggaggt aggaaatccc aattcccgga atttctcaaa ggcctcgtcc
     1261 cgcttatgat tgatcacatc agccgaatgt tttttaatcg tttcgtgatg ttgggtataa
     1321 aggtcaatat acccggcctc tcccaaccca cctgaaggcg aggaaattga agagatgttg
     1381 ttgtttttat ctaaattatt cattttataa atctttttta gacctctctt taggagggtt
     1441 taggtgggct tccagttctt ccttttcttt agccggaggt aagaaatttt cttccgttat
     1501 tacatttctg cccgtttgca gttccaattc cttacgtgca tttttcgcta tctttccccc
     1561 tttttttcct gccacagcat tctctttcaa tcccgttgcc tgttcggttt gagcaatttg
     1621 gcgagtggat agttcggcta atgctgtgaa aatcaattct gcttcactca tgtgatcgcg
     1681 caaattctga tttttcagtc ccttcagttg tttgtgttgt ttcaccgtta aatcactcca
     1741 ctcctgatgg ataatattgg tgaggtaggc aaattcatcc tgctctttta tcccggcatc
     1801 tttccagtag tcggtcaatt tgtttcgggt ttcctgtccg gtcatccgct gctgtatcca
     1861 cttttcgctt ctccccgatc gtttccagtt ttcacgcgcc cggtctatac tctgtgatgg
     1921 gtcttgaatt tcctgaatgc gctcataacc aacctttgcc agccattgtt tgaaaggttc
     1981 tgctttgggc gatggaatgg attgaatgat acggagaagt gtctttgtgt ctgccgcatc
     2041 agtttctcga ttctttccat catcagcaat cattttcaac cgtacgattt tttcgtacac
     2101 ttcactacct tctgatgtta actttttttt caaatcgctc caatatcgct ttggaatggt
     2161 actttccgtc agcacttcca ccacatcaat caccgaaaaa taccactgtt cttccgtttc
     2221 gtcccaatga gtacgaattt tcctgctttc aaataatttt attgaagttt cggtcataaa
     2281 tttgggaaaa taatgaattt attatctctg atgttcccct ttaggggtta ggggttaggg
     2341 ctttacttca accaatcata tcctttttct tccagttcga gcgccaggtt tttatcaccg
     2401 gttttcacta tttttccgtc gtaaagcaca tgtacaaaat caggaatgat ataatccagc
     2461 agccgttgat agtgggttat tacaatactg gcattgtcag gccgttttag tttgtttact
     2521 ccattggcta caatgcgcag tgcatcaata tcgaggccgc tgtcggtttc atcaagaata
     2581 gctaattttg gttcaagcat agccatctga aaaatctcgt tgcgtttctt ttcacctccc
     2641 gaaaatcctt cattcaccga gcggttggtc agtttattgt ccatttccac cagttccttt
     2701 ttctcacgca tcagacgcag gaaatcggta gccgcaatcg gttcctgacc gcggtatttc
     2761 cgttgttcgt tgatggccgc acgcataaag ttgaccatgc tcactccggg tatttccacc
     2821 ggatactgaa aactcagaaa aatacctgcg ctggcacgtt gctcggggtt gagttcaaag
     2881 aggtcatttc cctcaaagat cacttttccc tccgttacct caaatgccgg attgccggta
     2941 agcaccgagg caagcgtcga ttttccggca ccgttgggtc ccatgatagc atgtatttca
     3001 ccggcattga ccgtaaggtt caatcctttc agtatttctt tgccatttac attggcgtgt
     3061 aagtttttta tttgtaacat aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnntacccca cacttccttc cagtgtgatc tgcagcagct tttgcgcttc cacggcaaat
     3241 tccatgggca atttgttgag cacttccttg gcgtaaccat tcactatcaa cccaacagct
     3301 gtttcggtgt taatgccgcg ctgattgcag tagaatatct ggtcttcgct tattttcgaa
     3361 gtggtggctt cgtgttccac aatggccgta tcgttattga tgtccatata cggaaaagtg
     3421 tgtgctccgc atttatcacc aagcagcaga ctgtcgcact gtgaataatt acgggcattt
     3481 tcagctttcg gattaacacg caccagaccg cggtagctgt tgttgctgaa acccgccgaa
     3541 attcccttgg ataaaatatg actgctggta tttttaccga tatgcatcat tttggtaccg
     3601 gtatcagcct gttgatggtg attggtaaca gctacggaat aaaattcagc cgaggaatta
     3661 tcgcccagca ggatgcagga tggatatttc caggtaatgg cagagccggt ttcaacctgg
     3721 gtccacgaaa gctttgaatt ttcccctttg catattcccc gtttggtcac aaagttataa
     3781 ataccgccct ttccctcttt atcgccggga taccagtttt gaaccgtaga atatttcact
     3841 tcggcatttt tcatcaccac aatttccaca atggctgcat gcagctggtt ttcatcacgc
     3901 atgggtgctg tacaaccttc caggtaggaa acgtagctgt cctcatcggc cacgatcagt
     3961 gtccgctcaa actgcccggt attcatagca ttgatacgga aataagtgga aagttccatg
     4021 gggcagcgaa cccctttggg gatataaacg aaggagccgt cgctgaatac agcactgttc
     4081 aatgtggcaa aaaagttgtc ggtatagggc accaccgatg ccatgtattt ctgtaccaga
     4141 tcgggatgat gctccaccgc ttcgctgaag gagcagaaaa tgatgcccat ctccgccaga
     4201 ttttcacgga aagttgtttt caccgaaatg ctgtccatca cagcatccac cgccattccc
     4261 gatagcactt tctgttcatc gaggggaatg ccgagtttat taaaggtatt gagcaattcc
     4321 ggatccactt catccagact tttcggtgca ttttttcggg gtgctgcaaa ataggacata
     4381 ttctggtaat caatttccgg tatggtcaaa tgcgcccagg tcggcatctg catcgtgagc
     4441 cagtgtctgt aggatttcaa cctgaaatcc agcaaccatt cgggctcgtt cttcttggcc
     4501 gaaatcaagc ggatcacgtc ctcgttcagt cccatcggga ttacatccgt ttcaatatcg
     4561 gtggtaaaac cgtatttata atcgctttgg gttatttcat ctaatatatc gtctgacata
     4621 atggtgtttt attgaatata cttgcgtaat aacgtacaaa gataatatat tgttttcaaa
     4681 ttgtgagaat ttaatccctt actggccact ttacaacttc attatcaaca tgttgaattg
     4741 tattatttaa atttcatccg gttatttttt aaacttttag cggataaatc tgttttactt
     4801 tttaaattac aaatttgaaa tcaaattttt cagacgttta tcaggatctt attgatatt
//
LOCUS       DAOPZK010000142         4770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_22834_length_4770_cov_27.710923, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000142 DAOPZK010000000
VERSION     DAOPZK010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22834_length_4770_cov_27.710923"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="PKH68_07840"
     CDS             complement(<1..1789)
                     /locus_tag="PKH68_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNX12888.1"
                     /translation="MAKYKMHILLCGGTGCQSSGSSVILDNLQKELLMKGLSDEVQVI
                     RTGCFGFCEKGPIVNVLPDNTFYVEVKPEDAREIIDEHVIKGRQVKRLLYVDPENKEH
                     VPDSKHMGFYKKQLRIALRNCGFIDPENIDESIAHDAYMALGKVLTEMSPTEAIDIIK
                     KSGLRGRGGGGFPTGLKWEITSNSVSDKKYVVCNADEGDPGAFMDRSILEGDPHSVLE
                     AMAICGYCIGADEGLIYIRAEYPLAIKRLKIAIKQAEEYGLLGENILDTGFNFKIKLR
                     YGAGAFVCGEETALIHSMEGRRGEPTNKPPFPSVKGYLDKPTNVNNVETFANIPAIIL
                     KGADWFSSIGTEKSKGTKVFALAGKINNVGLIEVPMGITLREIIYEIGGGIKGGKKFK
                     AVQTGGPSGGCLTEKHLDTPIDYDNLIQAGSMMGSGGMIVMDEDDCMVSVAKFYLDFI
                     VEESCGKCAPCRIGNKRLYEILDKICDGNGTQEDLERLKNLSNVIKDTSLCGLGQTSP
                     NPVLSTMTNFWEEYKEHVHDKKCRSGHCKNLLYYVIDPEACIGCMLCVRNCPVFAITG
                     ERKQSHFIHQDKCIKCGACMEKCKFDAIHL"
     gene            complement(1808..2200)
                     /locus_tag="PKH68_07845"
     CDS             complement(1808..2200)
                     /locus_tag="PKH68_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNX12889.1"
                     /translation="MEKIKTLADLKRKQEAVKSQVNLRDRASRAENLVQVKVAMATCG
                     IASGAKEVMEYFVEELDKRNIEAVVTQTGCMGYCYAEPTVEVKLQDAEPIVFGYVDTK
                     KANEIIERYIKTGELVDGIIPKNYETIN"
     gene            complement(2274..2840)
                     /locus_tag="PKH68_07850"
     CDS             complement(2274..2840)
                     /locus_tag="PKH68_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX12890.1"
                     /translation="MVDLAMHMMDIAQNSIRAEAKNIEISFIENNTENTLIFAVKDDG
                     TGMSPETVAKLSDPFFTSRTTRKVGLGVPFLKMTCEQTDGKLEIKSEPGIGTEIRASY
                     NTNHPDCIPLGDLAGYMILLLIGNPGINFKFLYQLDDEKFELSTAELAENGIDDLSSA
                     EMSSAVKEFMNENLKELFGKREKESFLC"
     assembly_gap    2899..2998
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2999..3738)
                     /locus_tag="PKH68_07855"
     CDS             complement(<2999..3738)
                     /locus_tag="PKH68_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNX12891.1"
                     /translation="MIDLDYKKSPLEPVPNLFREDLGVEIGGSFKADLHIHTCLSPCG
                     DLEMSPRNIIQTAKEKGLDIIAITDHNSTRNVKTCMELGKKNNLFVIGGCEINTQEEV
                     HCLAYFRDLQLLNEFQQYLDEHLPDIKNDASLFGHQVAVDEFDNIIYEEEKLLIAALD
                     VDLETVAEKVLAMGGVFIPAHIDKSKNSIFSQLGFMPFGLKYSALEVSWRTSVEKFIE
                     KYPKLGKENFIQSSDAHYLEDIGKVYTE"
     gene            complement(3807..4151)
                     /locus_tag="PKH68_07860"
     CDS             complement(3807..4151)
                     /locus_tag="PKH68_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNX12892.1"
                     /translation="MEKKNLKELIEELDFKCVTCENLLDKEPKWAYVSDLLSDVMGKA
                     QPQMIWVTSQIHKNIVAVASLIDLSAIVIVNERKPDEDMLKLAEEEGVVVLTSDKPAF
                     ETAGMLYRYLAP"
     gene            complement(4120..>4770)
                     /locus_tag="PKH68_07865"
     CDS             complement(4120..>4770)
                     /locus_tag="PKH68_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNX12893.1"
                     /translation="SDSVKWSLSGTEREYFKGRSLAIDGVENVIKFLEKLESGHITDI
                     DFLELRVCDQGCAGGILCPGNRFLTVERIGERQKRLQERLIENKEVNNPLMKYKEQLH
                     RVSKTEAIYPREGLLLDADVEIALRKMQKIKTLKSYLPGFDCGACGAPTCKNLAEDIV
                     KGEASMSHCVFVQRVMEKNYKLSPEQAFQMIEKIWGKNRLNKYSENHGEKESERTD"
BASE COUNT         1125 a         1159 c          920 g         1466 t
ORIGIN      
        1 ttagatgaat ggcgtcaaac ttacattttt ccatacatgc tccgcattta atacacttat
       61 cctgatgaat aaagtgcgat tgttttcttt ctcctgtaat ggcaaataca gggcagtttc
      121 ttacacaaag catacatccg atacaggctt cggggtcgat tacgtaatac agcagatttt
      181 tacaatgacc cgaacggcat tttttatcgt gtacgtgttc cttgtactct tcccagaaat
      241 tggtcatggt tgaaagcacc ggattgggtg aggtttgtcc aagtccgcaa agtgaggtgt
      301 cttttatcac attgctcagg tttttcagtc gttcgaggtc ttcctgcgtg ccatttccat
      361 cgcatatttt atccagaatt tcatacaaac gtttattacc gatgcggcag ggtgcgcatt
      421 ttccgcaact ttcctccaca ataaaatcaa gataaaactt agcaaccgat accatacaat
      481 cgtcttcgtc catcacgatc attcctcccg aacccatcat ggagcctgcc tgaatgaggt
      541 tgtcgtaatc gatgggagta tcgaggtgtt tttcggtcag gcagccaccg gaagggcctc
      601 cggtttgcac ggctttgaat tttttgccgc ctttgatgcc gccgcctatt tcgtatataa
      661 tttcacgcaa cgtaattccc ataggtactt caatgagacc cacgttgttg attttaccgg
      721 ccagcgcaaa tactttggtc ccttttgatt tttccgtacc aatggacgaa aaccaatctg
      781 ctcctttgag aataatggca ggaatattgg caaatgtttc cacattgttt acgttggttg
      841 gtttatccag gtatcctttt accgatggga atggcggttt gttggtcggt tcgccgcggc
      901 ggccttccat ggaatgaata agggctgttt cctcgccgca aacaaatgct cccgctccgt
      961 aacggagttt tattttgaaa ttaaatcccg tatccaggat gttttcccca agcaatccgt
     1021 attcttcggc ttgtttgatg gcaattttca gccgcttgat agccagcgga tattcggcac
     1081 gtatatatat caatccttcg tccgctccga tgcagtaacc gcaaatggcc atagcttcga
     1141 gtaccgagtg cggatcgcct tcgagtattg agcgatccat gaaagctccc gggtcgccct
     1201 catcggcatt acaaaccacg tattttttat ccgaaacgct attgctggta atttcccatt
     1261 tcaatccggt agggaaaccg ccgcctccac gtccgcgcaa acccgatttt tttattatgt
     1321 caatggcttc ggtcggcgac atttccgtca gtactttccc caaagccata taggcatcgt
     1381 gagctatgga ttcgtcgata ttttcagggt caataaatcc gcagttgcgc aacgcaatgc
     1441 gcagctgttt tttataaaat cccatgtgtt tggagtctgg aacgtgttcc ttgttttcag
     1501 gatcgacgta gagcaggcgc tttacctgtc ggcctttgat tacatgttcg tcaataattt
     1561 cccgggcatc ttcgggcttt acttccacgt agaatgtatt gtcgggcagc acattgacaa
     1621 taggtccttt ttcgcaaaaa ccgaaacatc cggtgcggat tacctgtacc tcatcgctca
     1681 gtcctttcat gagcaactcc ttttgcagat tatctagaat gaccgaactg ccggacgatt
     1741 gacaacctgt gccaccgcat agtaatatat gcattttgta ttttgccata gtagaaatcc
     1801 tcctgcgtta gttgattgtt tcgtaatttt ttggaataat tccatccacc agctcaccgg
     1861 ttttgatgta ccgctcgatg atttcgttgg ctttttttgt gtccacatag ccaaaaacga
     1921 ttggttccgc atcctgtaat ttcacttcta cagtaggctc tgcgtagcaa tagcccatgc
     1981 agccggtctg agttaccacc gcttcgatgt ttcgtttgtc gagttcttct acaaaatact
     2041 ccatcacttc ctttgcaccg gaagcaattc cacaggtggc catcgccact ttgacctgaa
     2101 ccagattttc ggcacggctg gcccggtccc gaaggttaac ctgcgacttg actgcctcct
     2161 gcttacgctt cagatctgcc aatgttttaa ttttttccat gatattaaaa agattatgtt
     2221 tgttatagta tgtgtttttt tcagcaattt aaccaataac ctgtcaacta aactcagcat
     2281 aaaaaacttt ccttttccct tttcccgaac agttctttca gattttcatt cataaattct
     2341 ttcacggctg acgacatttc ggcactgctc aggtcgtcaa ttccattttc ggctaattct
     2401 gccgtactta attcaaattt ttcatcgtcg agctgataga gaaatttgaa attaattccc
     2461 ggatttccta tcaataataa aatcatataa ccggccaaat ctcccaacgg aatgcaatcg
     2521 ggatgatttg tattgtacga agctctgatt tctgtcccaa tgcccggttc ggattttatt
     2581 tcgagctttc cgtccgtttg ttcgcaggtc attttcagaa aaggaacacc caacccgact
     2641 tttcgggtag tgcgggaggt aaaaaacgga tcggacaatt tcgcgactgt ttcggggctc
     2701 attcccgtgc cgtcgtcttt tacagcgaaa atcaacgtat tttctgtatt gttttcaatg
     2761 aaacttatct caatattctt cgcttcggcg cggatcgaat tttgagctat atccatcata
     2821 tgcattgcca gatcaaccat tgcaaaagcc ccccagcccc ctaaaggggg aaaaaatctg
     2881 cactaaattt ctattattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     3001 ttcggtgtac accttaccga tatcctccaa ataatgcgca tccgaacttt ggataaaatt
     3061 ctcttttccc agcttgggat atttctcaat aaatttttct acacttgtgc gccacgaaac
     3121 ttccaatgca ctgtatttca gaccaaatgg cataaatccc aactggctga aaatgctgtt
     3181 tttcgactta tcgatatgcg ccggtatgaa aacaccaccc atcgccagca ctttttcggc
     3241 taccgtttcc agatccacat ccagggcggc tatcaacagc ttttcttcct cgtatataat
     3301 attgtcaaat tcatccaccg ctacctgatg tccgaacagc gaggcgtcgt tttttatatc
     3361 gggcaaatgt tcgtccagat actgctgaaa ttcattaagc agctgtaagt cgcggaaata
     3421 agccagacag tgcacctctt cctgtgtgtt gatttcgcat ccgccgatga cgaacagatt
     3481 attcttttta cccaactcca tgcaggtttt cacattccgg gtggagttgt ggtctgtaat
     3541 ggcaataatg tcaagccctt tttccttcgc agtttgtatg atatttcgcg ggctcatctc
     3601 caaatcgccg cagggcgaaa ggcatgtatg aatgtgaagg tcggccttga agctgccccc
     3661 tatctccacc cctaaatcct cccgaaataa attcgggaca ggctctaaag gggacttttt
     3721 gtaatctaaa tcaatcaaat tccggcaatt ttatggaaca aaacatttta tgttcttttg
     3781 aaaatcccac ttttcccctt taggggttag ggggctaaat acctatacag cattcccgcc
     3841 gtttcaaatg caggtttgtc ggatgtaagc acaactactc cctcttcctc tgccagtttc
     3901 agcatatctt catcgggttt tcgctcgttt acgatcacga tggcactcaa atcgatgagc
     3961 gaagccactg ccacaatgtt tttatgaatt tgcgaagtga cccagatcat ttgcggctgg
     4021 gcttttccca tcacatcact caatagatcg gacacataag cccatttcgg ctctttatcg
     4081 agcagatttt cacaggtcac gcatttgaaa tcaagttctt caatcagttc tttcagattc
     4141 tttttctcca tgattttcgg aatatttatt gagtctgttt ttgccccaga ttttctcgat
     4201 catctgaaaa gcctgttcag ggctcagttt gtagtttttt tccattaccc gctgtacaaa
     4261 tacgcaatgc gacatactcg cctctccttt tacaatatct tcggccagat ttttacaagt
     4321 tggtgcgccg caagctccgc agtcaaaacc gggaagatac gatttcaacg tcttgatttt
     4381 ctgcattttc cgaagtgcaa tttccacatc ggcatccagc agcaagccct cacgggggta
     4441 tatagcttcg gtttttgata cccggtgaag ctgttctttg tacttcatca gcgggttgtt
     4501 tacctcttta ttttcaatta gccgttcctg tagtcttttc tgcctttcgc ctatcctttc
     4561 tacggtgaga aatctgtttc cgggacaaag aatgccgcct gcacatccct gatcgcacac
     4621 acgcagttcc agaaaatcaa tatcggtaat gtgaccggat tccagctttt ctaaaaattt
     4681 gatcacattc tccacgccgt ctatggccag gcttctgcct ttgaaatatt cacgttcggt
     4741 accggacagt gaccacttga cagaatcgga
//
LOCUS       DAOPZK010000143         4665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_23525_length_4665_cov_22.525597, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000143 DAOPZK010000000
VERSION     DAOPZK010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23525_length_4665_cov_22.525597"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..2335)
                     /locus_tag="PKH68_07870"
     CDS             complement(65..2335)
                     /locus_tag="PKH68_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNX12894.1"
                     /translation="MKTILKFVFAFCIIFGSSANLFAQNSALQKANKLYELKAYAQAI
                     PKYLQVLKKDSSIADAVFNLADCYRLVNDARNAETYYGKCLKKTNSKPIHKFYYAQAL
                     MENGKVDLAKKYMKEFNDDDRGKTFYKALVNLEKFYKNTGDYKINIARFNSPQNDFSP
                     VIYKDKIVFVSARTRALVLNYKHSWTDLNYYFLYVTQKSGEDSYTKAKLFAKNIQTRY
                     NDGPVCFDRDNKTIYFTRNNLQNNKVVRSSNGEVKLSLFTAQINEEKKNLTNIEGFNF
                     NSKEYNTAHPAISADGKRLYFSSDMPGGQGGMDLWVVEKVNDAWGQVKNLGKKINTPG
                     NEVFPCITENNNLYFSSNGLEGIGGLDIFCVALDEAGKLKDSVRNMGAHLNSTADDFG
                     ITFYPGEKKGFFSSNRNSYDLNDEIFEFTIVKPEKKPYTILVMDSITRKLLESGIVIT
                     DTETGEKTTMTEKDGEFIANLFPQRTHRIDAKSEGYIPRNNIEYVTPLDNTEPFEILL
                     KQLPPKPKYFIAGTVYEILEDKSKIKVDSTLIQITDPGNNVVYSHITDTSGTYYAPDL
                     KPRTTYVVTASPPGFFNKSEIVDSIPKGGAIRDFYVSRIVVGKAIKIENIYFDYDKSN
                     IRPDAAIELNKIVKLLNENPAIIIELGSHTDCRGKKSYNEGLSDRRAKSSAAYIVSQG
                     IDPSRISGKGYGENVMIMNCPCEGAQKSNCSEEDHQLNRRTEFKVTGFVKGVGNVNMK
                     SDKPTNVITDPKPPEK"
     gene            complement(2363..3313)
                     /locus_tag="PKH68_07875"
     CDS             complement(2363..3313)
                     /locus_tag="PKH68_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNX12895.1"
                     /translation="MKYKSFKLVIGIIIISSFWNNGLKAQFDPMFTQYMNNEMFINPA
                     YAGAKEALAITLLHRQQWVSFPGRPITTTFSINTPLYKNKMGLGLAFLNEKIGVLNRN
                     LVYLSYAYRIQTGARSFLSFGVMGGLHLQINKLADVRPTETGDPSFSMNTPLVTTPNF
                     GFGMYFNTDKFYASFSIPRMLDDHITFNSAGTMIKGVSFKLKSWHYYLAAGRVFTVSD
                     NFKLKPHAMMKVTANAPVEFDINLDALIIEKLWLGLSYRSKADISAMAGIQILPTLLI
                     SYSYDYQLTSIKKFSGGSHEILLSYIFGYKGKKILSPRYF"
     gene            complement(3338..>4665)
                     /locus_tag="PKH68_07880"
     CDS             complement(3338..>4665)
                     /locus_tag="PKH68_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNX12896.1"
                     /translation="IICPTNITQITINGQCEADVPLINPTGYDMCSDTLSFTGIRSDG
                     LALSDPFTVGITTITWTATDGSGNTSTSCIQTVTVVSGPIANVDYDSTSQNTAVIIDV
                     LANDTDCDNNINPASVNTGCPECALPSNGTITINPGTGEITYTPDSNFTGTDTLIYEV
                     CDSGIPVLCDTALVIITVLPDTTHAAIGLAKSAVTIARQTNGSYNITFDITVENIGNE
                     DLHEVQVTDDLSATFPPPAVFTVIPAPTATGTLTANTAYNGASDINLLENLNSTLAAG
                     QIEHIEFMVNVRMYSFRDQTYKNTAIGEALDPLGEKVSDSSNNGFITDVNHNGIANEK
                     EENIPTPVSLKPWLFIPDGFSPNGDGINDLLVIPGIENYPDCELSIYNRWGNLIYRKK
                     QYDNSWDAKPNIGSMVVGKGMVQPGTYYYILEFNTEDMEPAKGYLIIQY"
BASE COUNT         1156 a          969 c         1081 g         1459 t
ORIGIN      
        1 ttccccaaaa caaaaccagc cccgtcattt ttactgcggg gctggtttca gatttttttc
       61 cgggttattt ttcaggcggt ttcgggtcgg tgatgacgtt ggtcggctta tcgcttttca
      121 tgtttacgtt gccgacacct tttacgaacc ctgttacttt aaactccgta cggcggttca
      181 gctgatggtc ttcttcggag cagtttgatt tttgtgcgcc ttcgcagggg cagttcatga
      241 tcatcacatt ttcgccgtaa cctttgccgc tgatacggct tgggtcaatg ccctggctga
      301 cgatatatgc ggcacttgat ttggcgcggc ggtcggagag gccttcgtta taagattttt
      361 ttccgcggca gtcggtatgc gaaccaagtt cgatgataat ggccgggttt tcgttgagta
      421 attttacgat tttattaagc tcaatcgcgg catccggcct gatattcgat ttgtcgtagt
      481 cgaaataaat attttcgatt ttgatggctt ttcctaccac aattctgctg acatagaaat
      541 cacggatggc cccgcccttg gggatagaat ccacgatttc ggacttgttg aagaatcccg
      601 gaggtgaggc ggtgaccaca taggttgttc ttggcttgag gtcgggggca taataagttc
      661 cggaggtatc ggtgatgtga gagtacacca cgttgtttcc ggggtcggta atttgtatca
      721 gggtgctgtc caccttgatc ttgcttttgt cttccaaaat ttcgtacaca gtgccggcga
      781 taaaatattt tggtttcggg ggcagttgtt tgagaagtat ctcaaagggc tcggtattgt
      841 ccaacggcgt tacatattcg atattattcc tgggaatata cccttcgctt ttagcatcga
      901 tcctgtgagt gcgttgcggg aaaaggttcg caataaattc tccatctttc tctgtcatgg
      961 ttgttttttc gcctgtttcg gtgtcggtga tcacgatgcc tgattcaagc aatttccggg
     1021 tgatgctgtc catgacgagg atggtgtaag gttttttttc gggttttacg atggtaaact
     1081 caaagatctc atcgttgagg tcataactgt ttctgttaga gctgaaaaac cccttttttt
     1141 cgccgggata aaaggtgatg ccgaagtcgt cggcagtgct gttgaggtgt gcgcccatat
     1201 ttctgacaga atcttttagc ttacctgcct catccagggc gacacaaaag atatcaagcc
     1261 cgccgatacc ttcgagtccg ttggaagaga agtataaatt attattctcc gtgatacagg
     1321 ggaacacttc gttgcccggt gtgttaattt tttttccgag gtttttgacc tgtccccagg
     1381 catcatttac tttttctacg acccacaggt ccataccacc ttgtcctccg ggcatgtcag
     1441 acgaaaaata cagccgtttg ccatcggcag agatagccgg gtgagccgta ttatattcct
     1501 tgctgttgaa attgaagcct tcgatatttg taaggttttt tttctcttca ttgatctgtg
     1561 ctgtaaacag gcttaattta acctcgccgt tagaggacct gacaacttta ttgttttgca
     1621 ggttgtttct ggtaaagtat atggttttgt tgtcacggtc aaaacatacc ggtccgtcgt
     1681 tgtaacgtgt ctggatattt tttgcaaata attttgcttt tgtatagctg tcttcaccgc
     1741 ttttctgggt aacgtataga aaataataat tcaggtctgt ccatgagtgt ttgtaattaa
     1801 gaacgagcgc cctggttcgt gcggaaacaa aaactatttt atctttgtaa ataaccggcg
     1861 agaaatcgtt ttggggagaa ttgaagcggg caatattaat tttataatcg ccggtatttt
     1921 tgtaaaactt ctcaaggtta accagtgcct tgtaaaaagt tttccctctg tcgtcgtcat
     1981 taaactcctt catatatttt tttgcaagat ccacttttcc gttttccatc agggcctgtg
     2041 cataataaaa cttgtgtatc ggctttgagt tggttttttt gaggcatttt ccataatacg
     2101 tctccgcatt acgggcatca ttcaccagtc ggtaacaatc cgcaagattg aagaccgcgt
     2161 cagcgatgct gctgtctttt ttgagcacct gcaaatactt tgggattgcc tgggcataag
     2221 ctttgagttc atataactta ttggcttttt gcaaggcgct gttctgtgca aaaagatttg
     2281 ccgaagagcc aaagatgata caaaaagcaa atacgaattt taaaatagtt ttcatggctt
     2341 ataaattatt attatataca tattagaagt atcgtggcga aaggatcttt tttcctttgt
     2401 atccaaagat gtaggaaagg agaatttcgt gcgagccacc cgagaatttt tttatagagg
     2461 tgagctggta atcgtaagaa taagagatga gcagcgtcgg aagtatctga atacctgcca
     2521 tggcgctgat atcggctttg cttcgatacg acagccccag ccacaatttt tcaataatca
     2581 gtgcatcaag gttgatgtca aattccaccg gagcattagc ggtcactttc atcatcgcat
     2641 ggggtttgag tttgaaatta tcactgacag taaaaaccct tccggcagcc aggtagtaat
     2701 gccatgattt cagtttgaaa gaaacgccct ttatcattgt cccggcgctg ttaaaagtga
     2761 tatggtcgtc gagcattctc ggtatagaaa aacttgcata aaatttatcc gtattgaaat
     2821 acatgccaaa cccgaagtta ggagtcgtca ccaggggcgt attcattgaa aaactcgggt
     2881 cgcctgtttc ggtggggcgg acatctgcga gtttgttgat ctgcagatgc aatccgccca
     2941 tcaccccaaa acttaaaaag ctgcgtgcac cggtttgtat gcggtatgca tagctcagat
     3001 acaccaggtt ccggttcagg acgccgattt tttcgttcag gaaagccagt cccagtccca
     3061 ttttattttt ataaaggggt gtgttgatag aaaaggtagt cgtaataggc cttcccggaa
     3121 aactaaccca ttgttgccgg tgcagcaaag taatggccaa cgcttcttta gcccctgcat
     3181 aagcgggatt tataaacatc tcattgttca tgtactgggt gaacatagga tcaaactgag
     3241 ctttaagccc gttattccaa aaacttgaaa tgatgatgat cccgatgact agtttgaatg
     3301 atttatattt cataatgtca atatttacag gttgttacta atattgtatt attagatatc
     3361 ctttagcggg ttccatatct tcggtattga attcaagaat ataataatag gtgcctggct
     3421 gaaccattcc ttttccgacg accatagacc ctatgttggg ttttgcatcc cagctattat
     3481 catattgctt ctttctgtag atgagattac cccagcggtt atatatagac agctcgcagt
     3541 cgggatagtt ttcaatgccc gggatcacta acagatcatt gataccgtcg ccatttggcg
     3601 agaaaccgtc aggaataaac aaccacggct tcagcgatac cggtgtgggg atgttttctt
     3661 ctttttcgtt agcaattccg ttatgattca cgtcggtgat gaacccgttg tttgaactgt
     3721 cgcttacctt ttcgcccaat gggtccagcg cctctccgat ggctgtgttt ttatatgtct
     3781 gatcgcggaa actgtacatc ctgacattga ccataaattc aatatgttct atttgtcctg
     3841 cagcaagcgt actgtttaag ttttcaagca agttgatgtc cgaagcgcca ttgtaagcag
     3901 tatttgctgt cagagtacct gtcgcggtcg gagcgggaat aacggtaaat accgcaggag
     3961 gcgggaatgt ggcagacagg tcatccgtta cctgaacttc atgaaggtct tcattaccaa
     4021 tattttctac tgtgatatca aaggtaatat tataggaacc gttagtttgc cttgcaatgg
     4081 ttacagccga ctttgccagg ccaatagcag catgtgttgt gtcgggcaac acggtaataa
     4141 tcactaaggc ggtatcgcac aaaacaggaa taccggaatc acaaacctca taaatcaaag
     4201 tatctgttcc ggtaaagtta gaatccggcg tataggtaat ctctccggtc cccgggttaa
     4261 tagtaattgt tccgtttgac ggcagtgcac attcagggca gcctgtattt acagaggcag
     4321 gattgatatt attatcacaa tcggtatcgt tagccagcac atcaataatt accgctgtgt
     4381 tttggcttgt ggaatcataa tcaacattgg ctataggtcc tgaaacgaca gtcacggttt
     4441 gtatacagct tgtgctggta tttccggaac catccgtcgc cgtccaggtg atagttgtaa
     4501 tacctaccgt gaaaggatca ctgagcgcca ggccatcgga acgtatgcct gtaaagctga
     4561 gggtgtcaga gcacatatcg taaccagtag ggttgatcaa tgggacatca gcctcacatt
     4621 gcccgttaat agttatctgt gtgatatttg tcggacaaat aatga
//
LOCUS       DAOPZK010000144         4615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_23910_length_4615_cov_14.747149, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000144 DAOPZK010000000
VERSION     DAOPZK010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23910_length_4615_cov_14.747149"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..2813)
                     /locus_tag="PKH68_07885"
     CDS             complement(237..2813)
                     /locus_tag="PKH68_07885"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNX12897.1"
                     /translation="MKCENTNLWIVNVHFEDNPGEINYRYSILKQDKTFENEFGNLRK
                     VLIPAKQQFIAIEDNRRIPNGDSAFNTVAFTDCFFKRKKTTKNPQSENINFVLRINCP
                     QMEPDRHFAIIGNQSVLGSWDVSRKIRLNESNFPFWSVALNIKDITFPLEYKYLIVDT
                     KTDEVLAWGGGPNRRVENAEMGKFTIVTDENFIRTIPSWKTAGVAIPVFSLRSEEGFG
                     IGELLDLKKMVDWAKQTGQHIIQTLPINDTILNHTNYDSYPYNAVSVYALHPVYLRLE
                     RLGTLKYKQDIAYFENIKKKLNENSFSDYQNVLNEKWKYFKLIYPKESPAVFTSDDYK
                     QFFEANKTWLVPYAVFSYLRDKNGTPEFGKWNEFNTYKQNEIEAFASEGTPYYDEIAL
                     FYFLQYHLHKQLVEVHEYARSKGVVLKSDIPIGVSPRSVDAWVEPQLFNTNMQAGAPP
                     DDFSVTGQNWGFPTYNWDEMAKDGYRWWQNRFRKFMEYFDAYRIDHILGFFRIWEIPE
                     KDVWALTGNFSPALPFSVQDLQTRGLWWDENRYLRPYLREHVVRAVFGKYADDVMHEY
                     LLTDGWQNFKFKPEYDTQKKIEAHFAALGYNFTTKEVAIRDGLYALHCEVLFVRDKEY
                     HNLFHPRISMHSSQTFKDLQDDTRKILDRIYVEYFYHRHTEFWKEQALKKLPALLAAT
                     NMLVCGEDLGMVPDSVPEVINRLEILSLEIQRMPKKPNTEFAMPADAPYLSVCTTSTH
                     DMSPIRAWWEEDAAITQRFYNHALCINGPAPEVCTPFIAEKIVEQHLKSSAMLVILPW
                     QDWMSMDEKLRYKYPNAERINVPSNPRNFWCYRMHLTLEKLLSEKKLNVKIKKLIEVS
                     GR"
     gene            complement(3054..3124)
                     /locus_tag="PKH68_07890"
     tRNA            complement(3054..3124)
                     /locus_tag="PKH68_07890"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3090..3092),aa:Cys,seq:gca)
     gene            complement(3286..3759)
                     /locus_tag="PKH68_07895"
     CDS             complement(3286..3759)
                     /locus_tag="PKH68_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12898.1"
                     /translation="MRNIKYILLSALLLLSLAIQAETEQRGRPNVKEMHERKWQFMMN
                     EVELSPAEINAVKPVFLNYEKAIWELHEKDREFFRLSRDRKQGVDINYSELNDKAVNS
                     EISHALLLKNYHLKLRKLLKPETLFNYYQAERKFKRKLLQNMPKNMPPPPPEYDR"
     gene            complement(3771..4097)
                     /locus_tag="PKH68_07900"
     CDS             complement(3771..4097)
                     /locus_tag="PKH68_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12899.1"
                     /translation="MMRKKNISLDEMDKRLPFTVPENYFEDFATSMELQISEVAAEKK
                     HRQLKPWVYAVAAVSTGLIIFGSVYTSVFSGTSKPDNYESYVLSQVDETSMLYYYLEG
                     HNTENK"
     gene            complement(4145..>4615)
                     /locus_tag="PKH68_07905"
     CDS             complement(4145..>4615)
                     /locus_tag="PKH68_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX12900.1"
                     /translation="YQERLYWHIRKMVLSHDDTNDILQDVFMKAWNGLDSFRGESQVS
                     TWLYRIATNETLTFLTNKRLKNINSLDEVEDILLQNLQADIYFNGDEAQLQLQKAILA
                     LPEKQRLVFNMRFYEDLSYDEMSEVLGTSVGALKASYHHATKKIEHFLIEENDD"
BASE COUNT         1245 a          967 c          888 g         1515 t
ORIGIN      
        1 ttttacaaaa agctttcgga tacagtccct tgtcaatgtt ttttatggca ttgtgaacat
       61 cttcctttga agccgaaacg ccccggagat cgtatttttc gtttgtcatt ggagtgaaat
      121 tttgagttgc aaaattagtg aaaataaaca ggaacgtcaa acaatttcat gtctgacgtt
      181 cattattcgt attttgtata accaacattt aattatcact tccggcaaaa aggatatcat
      241 ctgccgctga cctctatcaa ctttttaatt tttacattta atttcttttc gctcaataat
      301 ttttcgagtg tcagatgcat gcgataacac cagaaatttc tgggattgct cggtacattg
      361 attcgttcag cgttcggata tttgtaacgg agcttttcat ccatcgacat ccagtcctgc
      421 cagggcaata taacaagcat tgctgatgat ttcagatgct gttctactat tttctcggcg
      481 ataaatggcg tacatacttc gggagccggt ccgtttatac ataatgcatg gttataaaac
      541 cgctgtgtaa ttgctgcgtc ttcttcccac caggctcgta tagggctcat atcatgtgtt
      601 gaagtagtac aaaccgacag atatggagca tcagcaggca tggcaaattc tgtattcggt
      661 tttttaggca ttctttgtat ttcgagactg aggatttcga gccggtttat tacctccggc
      721 accgagtcgg gcaccatacc cagatcttca ccgcagacaa gcatattggt agctgccagc
      781 aacgccggaa gtttcttcaa tgcctgttct ttccagaatt cggtatggcg gtgataaaaa
      841 tattccacat atatccgatc cagtattttt cgggtatcat cctgcagatc cttaaatgtc
      901 tgcgaactgt gcatggaaat gcgtggatgg aataggttat gatattcctt atcccttaca
      961 aacaacactt cgcaatggag agcataaagc ccgtcacgga tagcaacttc tttggttgtg
     1021 aaattatatc caagcgcggc aaaatgagct tcgattttct tttgagtgtc atattcaggt
     1081 ttgaacttga aattctgcca gccatcggtc agcaggtact catgcatcac gtcatcagca
     1141 tattttccaa acacagcacg aactacatgt tcgcggagat acggtcgcag ataacggttt
     1201 tcgtcccacc aaagtccacg tgtctgcaaa tcctgcacac tgaaaggtag agcgggactg
     1261 aaatttccgg taagcgccca cacgtctttt tctggtattt cccatattcg aaaaaatccc
     1321 agaatatgat cgatccgata agcatcgaag tactccatga acttacggaa tctgttttgc
     1381 caccaccgat atccgtcttt tgccatttca tcccagttgt aagtcggaaa accccagttt
     1441 tgtcccgtca cggaaaaatc atcgggtgga gctccggctt gcatgttcgt attgaacaac
     1501 tgtggttcga cccaggcatc aacacttcga ggactcacac cgataggtat atcgcttttc
     1561 aggactacgc ccttactacg ggcgtactca tgaacttcaa caagctgctt gtgtaaatga
     1621 tattgcagga aataaaacaa ggcaatttca tcgtaatatg gagttccttc cgaggcaaaa
     1681 gcttctattt cattctgttt gtaggtgttg aattcattcc atttcccaaa ttcgggcgtt
     1741 ccattcttat cccgcaggta tgagaaaacc gcataaggca cgagccaggt tttattcgct
     1801 tcaaaaaact gtttgtaatc gtccgaggta aaaacagccg ggctttcttt cggataaatc
     1861 agtttgaaat acttccattt ttcgttcagt acattctgat aatccgaaaa actattttcg
     1921 ttgagtttct tttttatatt ctcaaaataa gcgatatctt gtttatattt cagcgttcca
     1981 agtctttcca gccgcagata aacgggatgc agggcatata ccgaaactgc attgtaggga
     2041 tatgaatcat aattggtatg attcagaatg gtatcgttaa tcgggagagt ctgtatgatg
     2101 tgctgtcctg tttgtttcgc ccagtcgacc atttttttca gatcgagcaa ttcaccaata
     2161 ccaaaacctt cctcactgcg tagtgagaaa accggtatgg caactccagc ggttttccag
     2221 gagggtattg tacggataaa attttcgtcg gttacgatgg taaatttccc catctctgca
     2281 ttttccactc tccggttagg accaccaccc catgccagta cctcatctgt ctttgtatct
     2341 acaattaagt atttatattc cagcggaaag gtaatgtctt tgatattgag agcaacagac
     2401 caaaaaggga agttcgattc gttcagtcgt atttttctgc ttacatccca gcttccgagt
     2461 actgactgat ttcctatgat ggcaaaatgg cgatcaggtt ccatttgagg acaattgatc
     2521 cgaaggacga aattgatatt ctccgactgc ggattttttg tggttttttt tcgcttaaaa
     2581 aagcaatcgg taaaagccac cgtgttaaat gcactatccc cgtttggtat tcgtcggtta
     2641 tcttctattg caataaactg ttgctttgcg ggtattaaaa ctttccggag atttccaaat
     2701 tcattttcga atgttttatc ctgtttcagg atagaataac gataattaat ctcacccgga
     2761 ttatcctcga aatgaacatt cacaatccac agattcgtgt tttcacattt catttcatgt
     2821 cggacaaatt gctgttcatc cgtgtataat tgcaggtaaa ttgattgccc ccagagtgtc
     2881 tgaaaattga gtctgaattg tatttgcata ggagtgagat ttattaatga gtacaaattt
     2941 actgaattta gatacccgac agtaaatata acactccaat aataaaaatg attactaaaa
     3001 cgtttgcgaa aaaaaacctt ctgaaatata tcgtttcaga aggttcttta ttgaggtact
     3061 tggcggattc gaaccgccgt atgcggtttt gcagaccggt gcctagccac tcggccaaag
     3121 taccctggtt ttggactgca aagatagtac tattttgtaa tatgtcaaaa tttcaattcg
     3181 gttttttgct ttgagctgtg ttccggataa aaaaaacaca gatttcgatg ttctctgatc
     3241 tgtgttttta gtcatatatt gagttgttgt tcctaaatga atcttttatc tgtcatattc
     3301 aggcggtggc ggaggcatat tttttggcat attctgcagc aattttcttt tgaatttccg
     3361 ttctgcctga taatagttga ataaagtttc gggttttaat agttttctta gtttcagatg
     3421 atagtttttc aacagtagag catgtgatat ctctgagttt acagctttat cgttcaactc
     3481 ggaataattt atatccacac cttgttttct gtctcttgac aaccggaaga attctctgtc
     3541 tttttcatgg agttcccata ttgccttctc ataattcaga aaaacaggtt tcacagcatt
     3601 tatttctgcc ggtgatagtt ctacctcgtt catcataaac tgccactttc tttcgtgcat
     3661 ttctttaaca tttggtcgac cgcgctgttc ggtttctgcc tgtattgcca gcgacaacag
     3721 caataatgca ctaagcagga tgtatttaat atttcgcata tctgttattt ttatttattt
     3781 tcggtattgt ggccttcaag atagtagtaa agcatagatg tttcgtcaac ctgcgacaaa
     3841 acatacgatt cataattatc cggttttgaa gttcctgaaa aaacagatgt gtacactgag
     3901 ccaaaaatga ttaaacctgt agaaacagca gctactgcat acacccaggg tttcagctga
     3961 cggtgtttct tttcagctgc aacttcactg atttgcagtt ccatactggt tgcaaaatct
     4021 tcaaaatagt tttcaggaac cgtaaacggc aaccttttat ccatttcatc cagcgatatg
     4081 tttttctttc tcatcatact tatcagactt ttattaacac taaaggttta aatatggcat
     4141 tatattaatc gtcattttct tcgattagaa aatgctcaat tttttttgtt gcatgatgat
     4201 acgaagcttt aagagctcca accgatgtac caagcacttc gctcatttca tcatacgaaa
     4261 gatcttcgta aaaacgcata ttgaatacca gtctttgttt ttcgggcaag gcaagaatcg
     4321 ctttctgtaa ctgaagttgt gcttcgtcgc cgttgaaata aatatcggcc tgaagattct
     4381 gaagcaggat atcttccact tcgtcgagcg aatttatatt tttcagcctt ttattggtca
     4441 gaaaagtgag cgtttcgttg gtcgctatcc gatatagcca ggtagagacc tgcgattcac
     4501 ccctgaaact gtccagtccg ttccaggctt tcataaacac gtcctgtaat atgtcgtttg
     4561 tatcatcatg tgacagcacc attttacgga tatgccaata caaccgctcc tgata
//
LOCUS       DAOPZK010000145         4610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_23954_length_4610_cov_22.108013, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000145 DAOPZK010000000
VERSION     DAOPZK010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23954_length_4610_cov_22.108013"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PKH68_07910"
     CDS             <1..1247
                     /locus_tag="PKH68_07910"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNX12901.1"
                     /translation="WKYQRFSSALATTDNKPIKVENPGRLNTNAGPDFINAKVRIGNT
                     LWAGNIEIHVNSSDWYVHRHHQDEAYGNIILHVVYHDDKPVLDRNGNEVPTLELKNHI
                     DEQLLSGYKDFAGSKNAIPCGSMAINVKEIIRSNWLEKLAIDRLQNKTETIFNKLSLN
                     INNWEQSFYEVFARNFGFSVNAEPFELMAKSLPQQYFAKSKNNLFQIEALVFGQAGLL
                     NKEFADEYPASLKTEFLYLQKKYGLTPIEGHLWRFLRIRPNNFPTIRLAQFASLIHQS
                     ENLFSKVIESRSLPEVYHLFDLTCSAYWHNHYVFDKAAEPSSKKPTKSTINLLIINTI
                     VPFIFAYGKTKNDENYTEKALDFLAALPPEKNRYTERYTATGIIPQNAMQSQAMIQMF
                     NDYCQPKKCLHCSIGDAILKQG"
     gene            complement(1261..>4610)
                     /locus_tag="PKH68_07915"
     CDS             complement(1261..>4610)
                     /locus_tag="PKH68_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNX12902.1"
                     /translation="GEVLNLKLPLMLQRLGNINVNGVFTGFYSNFVSNATLKSDLGTI
                     STDIIVRNENKIISYEGEVSTENFYLGQLLDAGGLLGKVSLVAQLGGSGLTADDADVI
                     FQANIDQVEFKGYNYTGISVNGTFMHKIFNGVIEVKDKNLEMVFNGEVDINDSLPDFD
                     FEADVAYANLKMLKITPKDTLSEVKGRLNIDFYGNNIDNLKGYIAINDASFHQNHKYF
                     DLRSFNLNTYISPTGYRTFDLKSDYVDAVFNGYFVFSELGNSFKKFLLNYLPNFYFAL
                     DTNNKNLARQEFNFDIRLYNATPVLDYFADGLEASGNTMVKGNFNSLTNHAKIDAVSS
                     KLSYKSFSLENWNLDFAAAEGGINLHMNSDTLHMSDSAYLSNVNLDSRGLNDSISSRL
                     QWDNKKATKRNAGNLNFYNYFYTNTQSVMGFKPSELVVNDSVWTISPQGNIVFDTGSI
                     ACNNLMLWYKEQNLTINGKISKNPVDVLAVEFNSFDISNVDYLTNAKKFDLDGIITGN
                     VFISDLYNSPTYFADLKIKDFGVNYDKLGDASIQSKWDDVKKGVHIKAEIYYIGNIGK
                     STPVIAEGYYYPGDKPQNFDIDISVENLRLKALSRYVASFGSIVSGTATGKLYLRGKQ
                     KPELSGLLKVMRGALKINYLNTVYAFAFDSVKITRDEFLFQNLIAFDQPDLVHREVDT
                     VLVSGSVTHENFKNLGFNINVEPKKALVLNTSASMNDLFYGKGYATGNARIYGTQNII
                     NFDISATTEKGTQLIIPVGNTSALQASEYITFITQGHHEDTISEEKGKAIKGINLDMS
                     FNVTDDAEVQLLFDPRVGDRILGRGNGNIRITIADNQMNMFGDYVLTSGDYLFTFQNI
                     INKRFLIDPGSTVKWNGDPYNAELDIRARYTLRANLAGLGVDTNTRYVNVDCIINMSN
                     VLSNPEFSFEVDIPSMQDYEKVPYLAAINQNINNNFISLLVINSFVNPNTGIGSATSA
                     GVSLLGKSASEVLSNQLSNWLSQISKNVNIGINYRPGDNISQEEVAVALSTQLFNDRV
                     TVSSNLGVATGQTATGENKNSNQIVGDVDIEVKISRALKLKVYNHTNQYSILQYTAPY
                     TQGIGIVYRKEFNTYRDLFLKNKNKKK"
BASE COUNT         1351 a         1007 c          919 g         1333 t
ORIGIN      
        1 tctggaaata tcaaaggttc agctccgctc tagctactac cgataacaaa cccattaaag
       61 tggaaaaccc cggaagatta aacaccaatg ccggtccgga tttcatcaat gctaaagtgc
      121 gcattggcaa caccttatgg gcaggcaaca tcgaaattca cgttaattcc tccgactggt
      181 acgtacaccg gcaccatcag gatgaggcgt atggcaacat cattctccat gtggtttatc
      241 atgatgataa acctgttttg gacaggaatg gaaacgaagt acccaccctg gaattaaaaa
      301 accacataga cgagcagttg ttgtcaggat acaaagattt tgccgggagc aaaaatgcca
      361 taccctgtgg cagcatggcc attaatgtca aagaaataat ccgctccaac tggcttgaaa
      421 aactagcaat tgatcgtttg caaaataaaa ccgagacgat attcaacaaa ctaagtttaa
      481 acataaacaa ctgggaacaa tcattttatg aagtatttgc gcgaaatttc ggcttcagtg
      541 tcaatgccga accttttgaa ctcatggcaa aatcgttgcc gcagcagtac ttcgcaaaaa
      601 gtaaaaacaa tcttttccag atcgaagcgc tggtttttgg ccaggccgga ctgctcaaca
      661 aagaatttgc cgatgaatat cccgcaagct tgaaaaccga atttctttac cttcaaaaaa
      721 aatacggatt aacacctatc gaaggacatt tgtggagatt tctcagaatc cgtcccaaca
      781 atttccccac catccgtctt gcgcaatttg ctagcctgat acatcaatca gaaaatcttt
      841 tcagcaaagt gattgaaagc aggtcattgc cagaggttta tcatttgttc gacctgactt
      901 gttcagccta ctggcataat cattatgtat ttgacaaagc tgccgaaccc tcctcgaaaa
      961 agccgacaaa aagtacaatt aatcttctta taatcaacac aatagtgccg tttatttttg
     1021 cctatggcaa aacaaaaaat gacgaaaact ataccgaaaa agctttggat tttcttgctg
     1081 ccttaccgcc cgagaaaaac cgctatacgg aacgctatac cgcaacaggc attattcccc
     1141 aaaatgctat gcagtcgcag gccatgatac aaatgtttaa tgattactgc cagccaaaaa
     1201 aatgcctgca ttgcagcata ggcgatgcta ttttgaaaca gggctgaaca ggaaccaaga
     1261 ttactttttc ttgtttttgt ttttcaggaa taaatcccgg taggtattaa actctttgcg
     1321 gtatacgata cctattccct gtgtataagg ggctgtatat tgcaaaatac tgtactggtt
     1381 tgtatggttg tataccttta gctttaatgc cctggatatt ttcacttcga tatccacatc
     1441 gcctactatc tgatttgaat ttttgttttc acctgttgct gtctgccctg tagcaacgcc
     1501 aaggttgctg gatacggtta cccggtcatt aaaaagttgt gtcgaaagtg ccaccgccac
     1561 ttcttcctgc gaaatattgt cgccgggccg gtaattaata ccgatattaa catttttgct
     1621 gatttgcgaa agccagttgc tcaattggtt tgaaagcact tcgctggcac tttttcccaa
     1681 caggcttacc cctgccgaag tggccgaacc tatacctgta tttggattaa caaaactgtt
     1741 gataaccagc agggatataa agttattgtt gatattctgg ttgatagccg ccaggtaagg
     1801 aactttttca taatcctgca tggagggaat gtccacctca aacgaaaatt ccggatttga
     1861 caggacattg gacatattga taatgcaatc cacatttaca tacctggtat tggtatccac
     1921 tcccaggcct gccaggtttg ccctgagcgt atatcgggca cggatgtcca gttcggcatt
     1981 ataagggtcg ccgttccatt taactgtgct gcccggatca attaaaaaac gtttgttgat
     2041 aatattctga aaggtaaaca ggtaatcgcc cgaagtgagt acataatctc caaacatatt
     2101 catttggtta tcagcaatag taatacgtat gttgccattt ccccttccca ggatgcggtc
     2161 gcccactctg ggatcgaata aaagttgtac ctcggcatca tcagttacat tgaatgacat
     2221 atccaggtta atgcccttga tggctttgcc tttttcttcc gaaattgtat cttcgtgatg
     2281 tccctgggtg ataaaagtaa tatattcgct ggcctgcaaa gccgatgtgt tgccaaccgg
     2341 gataatcagt tgtgtcccct tttcggtggt agcagaaata tcaaaattta ttatattttg
     2401 agtgccgtaa atacgtgcat ttcccgtagc ataacctttg ccataaaata aatcattcat
     2461 ggaggcactg gtgttcagca caagagcttt tttaggttca acattgatat tgaatccaag
     2521 attcttaaaa ttttcatggg taacactgcc cgataccagc acggtgtcaa cttcgcggtg
     2581 gacaagatcc ggctggtcga aggcaataag gttctgaaac aaaaactcat ccctggtaat
     2641 tttaacgcta tcaaaagcaa aggcatacac ggtattgaga taattaattt tcagggcacc
     2701 cctcatcact ttgagcaggc ctgataattc gggtttttgt tttcccctca gatacaattt
     2761 gccggtggcg gtaccgctga caatagaacc gaaactggct acataccgcg agagggcttt
     2821 cagacgaagg ttttcaacac taatgtcgat atcaaaattt tgcggcttat cacccggata
     2881 ataataaccc tcagctatca ccggggtgct cttgccaatg ttcccaatat aatatatttc
     2941 ggcctttatg tgtacccctt ttttcacgtc atcccatttg ctttgtattg atgcatcgcc
     3001 caatttatca tagtttaccc cgaaatcctt gattttcagg tcagcaaaat acgttgggga
     3061 gttataaagg tccgaaataa aaacgttacc ggttataata ccgtcaagat caaatttttt
     3121 ggcattggtc agataatcaa cattggaaat atcgaaagaa ttaaattcaa cagccagtac
     3181 atccacagga tttttggata ttttcccgtt gatggtcagg ttttgttctt tataccataa
     3241 catcaagttg ttgcaagcaa tgctgccggt atcaaacacg atattcccct gagggcttat
     3301 ggtccacacg gagtcattaa ccaccagctc ggatggtttg aaacccatta ccgattgggt
     3361 gttagtataa aaatagttat aaaaattcag gttgccggca tttctctttg tagctttttt
     3421 attatcccac tgtaagcggc tgcttatgct gtcattaagc cctctggaat ccaggtttac
     3481 attggaaaga taagcgctgt ccgacatatg cagcgtatca gaattcatat gaaggtttat
     3541 tccgccttca gcagccgcaa aatccagatt ccagttttcc agtgagaaac ttttatagga
     3601 tagttttgaa gaaacagcat ctattttagc atgattcgtc aatgagttga agtttccttt
     3661 caccatggta ttgcctgaag cttcgaggcc atcggcaaaa taatccagca caggagtagc
     3721 attgtacaac ctgatatcaa aattaaactc ctgacgggcc agatttttat tgttggtatc
     3781 aagggcaaaa taaaagttgg gaagatagtt aagcaggaac ttcttaaacg aatttccaag
     3841 ctccgaaaaa acaaaatatc cgttaaagac agcatccaca taatcggatt tcaggtcaaa
     3901 ggtgcgatac ccggtcggac tgatgtatgt attaaggtta aaggaccgca ggtcaaaata
     3961 tttatggttt tgatggaacg aagcatcatt gatcgcgata tagcctttga ggttatcaat
     4021 attgttgcca tagaagtcaa tattcaaccg acctttgacc tcggaaagcg tatctttagg
     4081 ggttattttg agcattttta gattagcgta ggccacatcg gcctcaaaat cgaaatcggg
     4141 cagcgagtcg ttgatatcta cttcgccgtt gaagaccatc tcaaggtttt tgtctttcac
     4201 ttcgatcaca ccattgaaaa ttttatgcat aaaagtgcca tttacagaaa taccggtata
     4261 gttataccct ttaaactcaa cctggtcaat gttcgcctga aaaatcacat cggcatcatc
     4321 agcagtcagc cccgagccac ccaattgggc taccaggctc actttaccaa gcaatccgcc
     4381 cgcatcaagc aattgtccga ggtagaaatt ttcggtggac acctcccctt cgtaagatat
     4441 gattttattt tcgtttctca caataatgtc ggttgaaata gtgcccaggt cggatttcag
     4501 tgtggcattg gaaacaaaat tggagtaaaa tcccgtgaaa actccgttta cgttaatgtt
     4561 gccaagtcgt tgcagcatta atggaagttt caggttaagc acttccccgc
//
LOCUS       DAOPZK010000146         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_24023_length_4602_cov_22.401583, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000146 DAOPZK010000000
VERSION     DAOPZK010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24023_length_4602_cov_22.401583"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKH68_07920"
     CDS             complement(<1..561)
                     /locus_tag="PKH68_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNX12903.1"
                     /translation="MKFTPETYKIPDIPMTSFIDADEIWDYLNNTVSTKERVREVIAK
                     SLSKKRLNLEETAVLINATDPELIQEIKEGAKTLKRNIYGNRIVLFAPLYVGNKCSNN
                     CTYCGFRASNTAEVRKTLSDEELVKEIEALEDNGQKRLILVYGEHAQYDAEYIAHTVN
                     VAYSVKKGNGEIRRVNINAAPMEIEGF"
     gene            792..1466
                     /locus_tag="PKH68_07925"
     CDS             792..1466
                     /locus_tag="PKH68_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNX12904.1"
                     /translation="MWLIHSSIGRKVIMSVTGIFLILFLTFHSIMNVTVLIGGDIYNA
                     ICEFLGANWYAVAASLVLALGFVCHILYASYLTWLNYKARGNDRYAVSKSQEGVKWAS
                     KNMFVLGLIIFGFLGLHLFQFWYKMQYAELTGIHTGDFHPADGTAFIVQLFSQPVYSI
                     MYIVWLAALWFHLTHGVWSALQSIGLNNKTWLPRVKCFANIYSTFIILLFMAIPVFFL
                     FGFRPM"
     assembly_gap    1512..1611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1612..3543
                     /locus_tag="PKH68_07930"
     CDS             <1612..3543
                     /locus_tag="PKH68_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNX12905.1"
                     /translation="DAKIPEGPLAEKWSNYKAHQKLVNPANKRRLDIIVVGTGLAGAS
                     AAASLAELGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKGGD
                     YRAREANVYRLAEVSNSIIDQCVAQGVPFARDYGGLLDNRSFGGAQVSRTFYAKGQTG
                     QQLLLGAYSALSRQVGKGAVKLYSRYEMLDVVVIDGRARGIIARNLVTGKTERFFAHA
                     VVVGTGGYGNAFFLSTNAMTSNGSAAIQMYKKGAYFANPAFAQIHPTCIPVHGDYQSK
                     LTLMSESLRNDGRIWVPKKKEDAEAIRAGRLKPTDLKEEDRDYYLERRYPAFGNLVPR
                     DVASRAAKERCDAGFGVGNTGLAVYLDFSEAINRLGENVVRARYGNLFQMYEKIVNDN
                     PYNTPMMIYPAIHYTMGGVWVDYELQTSVKGLFCIGEANFSDHGANRLGASALMQGLA
                     DGYFVLPYTIQNYLADQIQVPRISTESKEFNEAEKAINDKIAKIMSIQGKLSVDHIHR
                     QLGLVMWDFVGMGRTAEGLKTGIEKIAQIRKEFWSDVYVPGKADNLNVELEKALRLAD
                     FIEIGDLMARDALLREESCGGHFREEYQTPEGEALRDDDKFAFVSCWKFKGEGQEPEL
                     IKEPLDYEFIVRQQRNYKA"
     gene            3655..4410
                     /locus_tag="PKH68_07935"
     CDS             3655..4410
                     /locus_tag="PKH68_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNX12906.1"
                     /translation="MEKSLNLTLKVWRQAGPKAKGSFETYKVEKISTDASFLEMMDIL
                     NEKLIGEHKEPIAFDHDCREGICGMCSMYINGHPHGPDGEITTCQLHMRNFADGDTIT
                     IEPWRSAAFPVIKDLMVDRSAYDKIIQAGGFVSVNTGGTPDANAIPISKVNADEAMDA
                     ASCIGCGACAAACKNGSAMLFVAAKVSQLALLPQGRVEAAHRAKAMVAKMDELGFGNC
                     TNTGACEAECPKNVSISHIARMNREFIGAKFKD"
BASE COUNT         1203 a          998 c         1035 g         1266 t
ORIGIN      
        1 gaaaccttca atttccatcg gagcggcgtt gatattcacc cggcggattt cgccgtttcc
       61 ttttttcacg ctgtatgcca cattaactgt gtgtgctatg tattccgcat cgtattgcgc
      121 atgttcgccg taaaccaaaa tcagccgttt ctgcccgtta tcttccagcg cttcaatttc
      181 tttaaccaat tcttcatccg aaagggtttt gcgcacttct gctgtgtttg atgctctgaa
      241 accgcaataa gtacagttgt tcgaacattt attgcccaca tagagcggtg caaagagcac
      301 aatccggttg ccatagatgt ttcttttcag cgtttttgcc ccttccttta tttcctgaat
      361 gagttccggg tcggtagcat taatcagcac agccgtttct tccaggttta atcttttctt
      421 ggataatgat ttggcaatca cttctctgac acgctctttg gtggaaacgg tgttgttcag
      481 ataatcccat atttcatccg catcgataaa ggaagtcatc ggtatatcgg ggattttata
      541 ggtttcagga gtgaatttca tagtggtaaa gtttatgtaa caaagataat gatagattat
      601 caaattaagg aattatttcc ttaattattt ttttaatcaa gggcaaatga gtaattttgt
      661 aaccgatttc aacaattcac attctccgtt accgaaagtc cggtttttac ccaaaatctg
      721 ctttccggtt ttttttctga ataaaattta attaaatact aactatattt caaactaaaa
      781 acagaaaatc tatgtggtta attcattctt ctatcgggag aaaggttatc atgagtgtaa
      841 ccggaatttt tctcattctt ttcctgactt ttcacagcat aatgaatgtg acggtgctta
      901 tcggcggcga catttacaat gcaatctgtg aatttttagg agcaaactgg tatgcagtag
      961 cagcctcgct tgtactcgca cttggtttcg tatgtcatat tttgtacgct tcgtacctga
     1021 catggctcaa ctacaaagcc cgtggaaatg accgttatgc cgtttctaaa agtcaggaag
     1081 gcgtaaaatg ggcctccaaa aacatgtttg tcctcggtct gattattttc ggattcttgg
     1141 gattgcacct cttccagttt tggtacaaaa tgcaatatgc tgaattgacc ggcattcata
     1201 ccggtgattt ccatcctgcc gatggtacag cctttattgt tcagctgttc agtcagccgg
     1261 tgtacagtat tatgtacatt gtttggttgg cagcgctgtg gtttcacctt actcacggtg
     1321 tgtggagcgc tttacagtca atcggactga ataacaaaac ctggttgccc cgcgtcaaat
     1381 gttttgcaaa catttattcc accttcatta ttttattgtt catggctatt ccggtgtttt
     1441 tcctgttcgg atttcgtccc atgtaatttg ttaaccgatt aaattactat taagattatg
     1501 gcaaaaaaag annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacgccaaa
     1621 atccctgaag gacccttggc cgaaaaatgg agcaattaca aagctcatca gaaacttgta
     1681 aatcccgcca acaaacgccg tctggatatc atcgttgtag gtaccggcct tgcaggtgct
     1741 tcagcagcag cttcgttggc tgagttgggt ttcaatgtgt tgaacttctg tattcaggac
     1801 agtccgcgcc gcgcacactc catagctgct cagggcggta tcaatgctgc caagaattat
     1861 cagaatgacg gtgactcagt gtaccgtttg ttttatgata ccatcaaagg cggtgactac
     1921 cgtgcccgcg aagccaacgt ttatcgtctg gccgaagttt caaacagtat tattgaccag
     1981 tgtgtggctc agggagttcc ttttgcacgc gattacggcg gtctgctcga caaccgttcg
     2041 tttggtggtg ctcaggtatc acgtactttc tacgccaaag gacaaaccgg acaacaactc
     2101 ttgctgggag cttattctgc cttgagccgt caggtgggaa aaggtgctgt taagctttac
     2161 tcacgctatg aaatgctcga tgtagtcgtt atcgacggtc gtgcacgtgg tattattgcc
     2221 cgcaacctgg ttacagggaa aaccgaacgt ttcttcgcac atgcagtggt tgtgggaaca
     2281 ggtggatacg gaaatgcatt cttcctctca accaatgcca tgacatcgaa cggatctgca
     2341 gctattcaga tgtataagaa aggagcatac tttgctaatc ctgcttttgc tcagatacac
     2401 ccgacctgta ttcccgttca tggcgactat cagtcaaaac tgacactgat gtccgaatcg
     2461 ctccgtaacg atggacgtat ctgggttcca aagaaaaaag aagatgccga agccattcgt
     2521 gccggcagac tgaaaccaac cgacctgaag gaagaagacc gcgattatta cctggaacgc
     2581 cgttatccgg ctttcggtaa cttagtgccg cgcgacgtag cttcacgtgc tgccaaagaa
     2641 cgctgtgatg caggatttgg cgtaggtaac accggtctgg ctgtttacct ggatttctcg
     2701 gaagccatca accgcctcgg cgaaaatgtg gttcgtgccc gctacggtaa cttattccag
     2761 atgtacgaaa aaatcgtgaa tgataatcca tacaatactc cgatgatgat ttatcccgct
     2821 attcactaca caatgggtgg tgtttgggta gattatgaac tgcaaacatc ggtaaaaggt
     2881 ttattctgta tcggtgaagc caatttctcc gatcacggag ccaatcgtct cggcgcttct
     2941 gcactgatgc agggactggc cgacggatat ttcgtgcttc cttatacaat ccagaactac
     3001 ctggccgatc aaattcaggt tccgcgcatc agtaccgaaa gcaaagaatt taatgaagct
     3061 gaaaaagcga ttaatgataa aattgccaaa ataatgagca ttcagggcaa attgtcggtt
     3121 gatcacattc accgtcagtt gggtcttgtt atgtgggact ttgtaggcat gggacgtact
     3181 gctgaaggac tgaaaacagg tatcgaaaag attgcccaaa tccgtaagga attctggtca
     3241 gatgtatatg ttcccggcaa agccgacaac ctgaacgttg aacttgaaaa agccctccgt
     3301 ttggccgatt ttattgagat tggcgatctg atggcacgcg acgcactgtt gcgcgaagaa
     3361 tcctgcggtg gtcacttccg tgaagaatac cagacccctg aaggcgaagc acttcgcgat
     3421 gacgataagt ttgcatttgt gtcctgctgg aaattcaaag gcgaaggcca ggagcccgaa
     3481 ctgattaaag aaccgctcga ttacgaattc attgtaagac agcaacggaa ttataaagca
     3541 taaaaaggct gcccccaacc ccctaaaggg ggagatggga gcgtgcaaaa tatcaaaaat
     3601 ttataaattt attaacttct aaattccccc tttagggggt tagggggcaa acatatggaa
     3661 aaaagtttaa atctcacgct aaaagtatgg cgccaggccg gtcctaaagc aaaaggcagc
     3721 tttgagacct ataaggtgga aaagatatcc accgatgcat catttctgga aatgatggat
     3781 attctcaacg aaaaactgat tggtgaacac aaagaaccga ttgcctttga tcacgattgc
     3841 cgcgaaggta tctgcggtat gtgcagcatg tatatcaacg gacatccaca cggacccgac
     3901 ggtgaaatca ctacctgcca gcttcacatg cgcaatttcg ctgatggtga caccattacc
     3961 atcgaaccgt ggcgttcggc tgcattcccg gttatcaaag acctgatggt ggatcgcagt
     4021 gcctacgata aaattattca ggccggtggt ttcgtttctg tcaataccgg tggaactccc
     4081 gatgccaatg ctattcctat ttccaaggta aatgccgacg aagccatgga tgctgcatcg
     4141 tgtatcggtt gcggtgcatg tgctgctgcc tgtaaaaatg gttctgccat gcttttcgtt
     4201 gccgcaaagg taagccagct tgccttgttg ccacagggta gggtagaagc tgctcaccgt
     4261 gcaaaagcaa tggttgccaa aatggacgaa cttggtttcg gtaactgtac caataccggt
     4321 gcctgcgaag ccgaatgtcc gaagaacgtt tccatctcgc acattgcgcg tatgaacaga
     4381 gaatttatcg gagcaaaatt caaagactaa aaaaccggcc tcttaattcc cataaaggga
     4441 aatagttgag gaaatataaa aattgaaagt ccgttgcaga agtgcagcgg actttttttg
     4501 gtgtttgaat gaaaaagaaa gaggcctcac cccgcttaac gttttatgat taagagactt
     4561 attacacccc tctcctcgag gagaggggac gggggtgagg tg
//
LOCUS       DAOPZK010000147         4536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_24551_length_4536_cov_21.752957, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000147 DAOPZK010000000
VERSION     DAOPZK010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24551_length_4536_cov_21.752957"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="PKH68_07940"
     CDS             complement(<1..118)
                     /locus_tag="PKH68_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNX12907.1"
                     /translation="MGARKKITADARKEANKTLYFAKLNGVPTSPRKMRLVAD"
     gene            complement(166..432)
                     /gene="rpsS"
                     /locus_tag="PKH68_07945"
     CDS             complement(166..432)
                     /gene="rpsS"
                     /locus_tag="PKH68_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNX12908.1"
                     /translation="MSRSLKKGPFINLKLEKKVLDMNETGKKTVVKTWSRATMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFSPTRIFRGHGGKKK"
     gene            complement(455..1279)
                     /gene="rplB"
                     /locus_tag="PKH68_07950"
     CDS             complement(455..1279)
                     /gene="rplB"
                     /locus_tag="PKH68_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNX12909.1"
                     /translation="MAVRKLKPSTPGQRHKIIGTFDTITASAPEKSLVFGGGKSGGRN
                     HDGKMTMRYIGGGHKRKYRVIDFKRNKDGVPATVKTIEYDPNRSARIALLFYADGEKR
                     YILAPNGLQVGQVILSGAEAAPEVGNALLLQNIPLGTVVHNIELRPGQGASMARSAGT
                     FAQLTSREDKYVIVKLPSGEIRKVLGTCKATIGSVGNSDHALERSGKAGRSRWLGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRHPKKQSSQYIIERKKK"
     gene            complement(1381..1671)
                     /gene="rplW"
                     /locus_tag="PKH68_07955"
     CDS             complement(1381..1671)
                     /gene="rplW"
                     /locus_tag="PKH68_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNX12910.1"
                     /translation="MAIIIKPIVTEKMTQLGDKLNRFGFRVQKDANKIEIKKAIEQAY
                     NVTVTDVNTFIVAPKKKSRFTKAGLVKGATSAYKKAIVTLKEGDKIDFYSNI"
     gene            complement(1684..2313)
                     /gene="rplD"
                     /locus_tag="PKH68_07960"
     CDS             complement(1684..2313)
                     /gene="rplD"
                     /locus_tag="PKH68_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNX12911.1"
                     /translation="MELNILDIKGKETGKKVSLDDAIFGIEPNDHAIYLDVKQYLANQ
                     RQGTHKTKGRSELSGSTRKLGKQKGGGGARPGDIKSGVRVGGGRMFGPVPRDYNFKLN
                     KKVKQLARKSALSYKALENAITVVDTLNFEAPKTKEFVAMTKSLQVADKKTLFILATG
                     NKNVYLSARNLATSKVITVSELNTYSILHAKKLVFTEESVAALEQLIKA"
     gene            complement(2316..2936)
                     /gene="rplC"
                     /locus_tag="PKH68_07965"
     CDS             complement(2316..2936)
                     /gene="rplC"
                     /locus_tag="PKH68_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNX12912.1"
                     /translation="MPGLLGKKIGMTSVFSADGKNVPCTVIEAGPCVVTQIKTVETDG
                     YKAVQVGFQEKTEKHTNKAEAGRFKAAGVAPQRHLVEFNEFEQEFKLGDVITVDTVFE
                     ADTFVDVIGTSKGKGHQGVVKRHGFGGVGQATHGQHNRLRAPGSLGASSWPSRVFPGM
                     RMGGRTGGDRVTMQNLQVLKVIPEHNLILVKGSVPGAKGSIVIINK"
     gene            complement(3082..3387)
                     /gene="rpsJ"
                     /locus_tag="PKH68_07970"
     CDS             complement(3082..3387)
                     /gene="rpsJ"
                     /locus_tag="PKH68_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNX12913.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKAREQFELSSYKRLIDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            complement(3517..>4536)
                     /gene="fusA"
                     /locus_tag="PKH68_07975"
     CDS             complement(3517..>4536)
                     /gene="fusA"
                     /locus_tag="PKH68_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853959.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX12914.1"
                     /translation="KQNPVELISAGDIGAGVGFKDIRTGDTLCDEAHPITLESMDFPA
                     PVIGISVEPKTQKDLDKLGMGLAKLAEEDPTFTVHTDEQSGQTVISGMGELHLEIIID
                     RLKREFKVECNQGRPQVAYKEAITQSVEIREVYKKQSGGRGKFADIIVKVGPADKDFE
                     GSLQFIDVVKGGNIPKEFIPSIQKGFQLAMKNGVLVGFPMDSLKVTLIDGSFHAVDSD
                     QLSFEICAQIAYKTACAKAKPVLLEPIMKMEVVTPEENMGDVIGDLNKRRGQVEGMES
                     SRTGARIVKAKVPLSEMFGYVTALRTISSGRATSSMEFSHYAEVSNAIAKQVVTEAKG
                     KVELL"
BASE COUNT         1147 a          984 c          858 g         1547 t
ORIGIN      
        1 tgtccgccac aaggcgcatt ttgcggggtg atgtaggaac accgttgagc ttggcgaaat
       61 aaagcgtttt gttagcttcc tttctggcat cggctgttat tttttttctt gcacccattg
      121 ttattctgaa atttttttat ttttttcttt gattaattca atggattatt tcttcttacc
      181 accgtgaccg cggaagatac gagtgggtga aaattcgccc agtttgtgtc ctaccatatt
      241 ttcggttata tatacgggga tgaatttatt tccattgtga actgcaattg tatgacctac
      301 aaaatcaggt gatatcattg tagcgcggga ccatgtctta accacggttt ttttaccggt
      361 ttcattcata tccagcactt ttttctccaa cttcaagttg atgaatggtc ctttttttaa
      421 cgaacggctc atattattta cttaattttt ctgattattt tttctttctt tcaataatat
      481 attggctcga ttgcttttta ggatgacgtg ttttgtatcc ttttgctaac aagcctttac
      541 gtgaacgtgg gtgtccacct gacgcacggc cttcgccacc tcccatgggg tgatcaaccg
      601 ggttcatggc cacacctctc acgcgtggac gacgtccaag ccaacgagaa cgacctgctt
      661 ttcctgatct ttccaatgcg tggtctgagt ttccaacgct tccaatagta gctttgcaag
      721 taccgagtac tttgcgaatt tcaccggaag gtaacttaac aatcacatat ttatcttctc
      781 tcgaagtcaa ttgtgcaaag gttcctgccg aacgagccat agaagctcct tgaccgggac
      841 gaagttcgat attgtgaact actgttccga gcggaatgtt ttgcagcaat aatgcattgc
      901 caacttcagg ggctgcttca gctcccgata gaattacttg acctacctgc aatccgtttg
      961 gtgcaagaat gtaacgtttt tcgccatcag cgtagaacaa caaagcaata cgggccgaac
     1021 gattcggatc gtattcaatt gttttcactg tagctggtac accatctttg ttacgtttga
     1081 agtcaattac tctgtatttt cgcttgtgtc cgccaccgat atagcgcatg gtcattttac
     1141 catcatggtt acgaccacca cttttaccac cgccaaatac aagtgatttt tctggtgcac
     1201 ttgcagtaat tgtgtcaaat gtaccaataa tcttgtgtct ttgccccggt gtagagggtt
     1261 ttaatttacg tacagccatt tatatattta ctattttatg atttacaatt taatcatttt
     1321 gttttatcat ttacaaactt actttcactg gtaaatcgta aatgataaaa ttgtaaatgt
     1381 ttagatattg ctataaaaat ctattttatc tccttctttc aatgtaacga ttgctttttt
     1441 gtaagcagaa gtagcacctt tgacaagacc ggctttggta aagcggcttt tctttttggg
     1501 agctactatg aatgtgttca catctgtaac agttacattg tatgcctgct cgattgcttt
     1561 tttaatttca atcttgtttg catctttttg aactctaaag ccaaagcggt tcagcttatc
     1621 gcccagctga gtcatctttt ctgtaacgat tggttttatg attattgcca tctttgtacc
     1681 tccttaagct ttaattaatt gttctaatgc agcaaccgat tcttcggtaa acactaattt
     1741 cttcgcatga agaattgagt aagtatttaa ttctgaaaca gttattactt ttgatgtcgc
     1801 taaattacga gccgacaaat atacgttttt atttccggtt gctaaaataa aaagtgtctt
     1861 tttatcagca acttgcaaac tttttgtcat tgctacgaat tctttcgttt ttggtgcttc
     1921 aaaattcagg gtatcaacaa ctgtaatggc attttcaagc gctttatacg aaagggctga
     1981 tttacgtgcc agctgtttta cttttttgtt gagtttgaag ttgtaatcac gtggcacagg
     2041 tccgaacata cgtccaccac ctacacgaac acctgacttg atgtcacccg gacgagctcc
     2101 accaccacct ttttgctttc ctaacttacg ggtactacct gaaagctcgc tgcgaccttt
     2161 cgttttgtgt gttccctgac gttgattagc caaatactgt ttcacatcta aataaatagc
     2221 atggtcattt ggctctatac cgaatatagc atcgtctaac gacacttttt tgccggtttc
     2281 ttttccttta atgtctaaaa tattcagttc catggttatt tattgatgat tacgattgaa
     2341 ccttttgcac ccggtacaga tcctttaaca agaatcagat tgtgttcggg tattactttt
     2401 aatacctgca aattttgcat agttactcgg tcgccacctg tacggccacc catacgcatt
     2461 cccgggaata cacgtgaagg ccatgacgat gcaccgagtg atcccggagc gcgaagacgg
     2521 ttgtgctgac catgagtagc ctgacctacc ccaccaaaac catggcgttt tactacaccc
     2581 tgatgtcctt taccttttga ggttccgata acatccacaa atgtatcggc ttcaaaaact
     2641 gtgtcaacgg tgattacatc acccaatttg aattcttgtt cgaattcgtt gaactcaacc
     2701 aagtggcgct gcggagctac tcctgctgct ttgaaacgtc ctgcttcagc tttgttggta
     2761 tgtttttcag ttttttcctg aaaacccacc tgaacagctt tgtatccgtc tgtttcaaca
     2821 gttttaattt gagtaacaac acaaggacct gcttcgataa cagtgcatgg tacattttta
     2881 ccatcggcac tgaaaacgga tgtcattccg attttttttc ctaataatcc tggcatttca
     2941 aatttaaatt aacctgcccc ctaaccccct aaagggggaa ttattgggta ggtatgatga
     3001 ataattattt tgttttaatt aataaaacct taagagaccc ctttttaatc tcccccttta
     3061 gggggccggg gggcttgttc cttatacttt aatttccact tctacaccgc tgggcagttc
     3121 gagtttcatc aatgcatcca ctgttttgct cgttgagctg taaatgtcga taagacgttt
     3181 gtaagatgag agttcgaatt gttcacgtgc tttcttgtta acgaaagtag aacggttcac
     3241 cgtaaaaatg cgtttgtgag ttggaagtgg aattggacca cttactactg cacctgtagc
     3301 tttcacagtt tttacgattt tttctgccga tttgtcaacc agattgtgat cgtaagattt
     3361 cagtttaatt ctgatttttt gactcattgt ctgtttttta attattaatt gtacatttat
     3421 tattgttgcc ggttaagagt cattcgctaa ccggtttttc acatatttac atataaacaa
     3481 tagtttgtga gcgaaatcac aaactattgt tattatttat aaaagttcaa cttttccttt
     3541 agcttctgtt accacctgtt ttgcgatagc atttgatact tcggcataat gtgagaactc
     3601 catggaagaa gtagcacgac cggaagaaat ggtacgtaat gcagttacat aaccgaacat
     3661 ttctgaaagc ggcactttgg cttttacaat ccgggcaccg gtgcggcttg attccatgcc
     3721 ttcaacctgt ccacgacgtt tgttcaagtc accgattaca tcacccatgt tttcttcggg
     3781 tgtaaccact tccatcttca tgattggttc caaaagaacc ggttttgctt tagcgcaggc
     3841 agttttataa gctatctgag cacaaatttc gaatgataac tggtctgaat cgacagcatg
     3901 gaatgaaccg tcaatgagtg ttactttcag cgaatccatc gggaagccaa ccaatacacc
     3961 atttttcata gcgagttgaa atcctttctg tatggaaggt ataaattcct tgggaatatt
     4021 accgcctttc accacatcga taaactgaag tgatccttca aaatccttat cggccgggcc
     4081 tacttttacg ataatatcgg cgaatttacc acgacctccg gattgtttct tataaacttc
     4141 ccgaatttcc acagattgag taattgcttc tttgtaagcc acctgtggac gaccctgatt
     4201 acattccact ttgaattcac gtttcagacg gtcgataata atttcgaggt gaagttcacc
     4261 cataccacta ataacggtct gacccgattg ttcgtcggtg tgaactgtaa atgttggatc
     4321 ctcttcggcc agtttggcaa gtcccatacc cagtttgtct aagtctttct gagtttttgg
     4381 ttctacagaa ataccgataa caggtgccgg gaagtccatt gattcaagtg taataggatg
     4441 cgcttcgtcg caaagcgtat cacctgtacg aatatcctta aatcctacac ctgcgccaat
     4501 atcaccggca gagatgagtt caacgggatt ttgttt
//
LOCUS       DAOPZK010000148         4526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_24634_length_4526_cov_24.071125, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000148 DAOPZK010000000
VERSION     DAOPZK010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24634_length_4526_cov_24.071125"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..415
                     /locus_tag="PKH68_07980"
                     /pseudo
     CDS             286..415
                     /locus_tag="PKH68_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640472.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            720..3170
                     /locus_tag="PKH68_07985"
     CDS             720..3170
                     /locus_tag="PKH68_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HNX12915.1"
                     /translation="MKRIFLINTRLVTILLFVSFLFQQNLKAQVSKLKVIPEVQQFTA
                     SSGTTSMSRKVKILVETVHSDSLMPIALQLKDELKTMMNITASIAETNSTTSKKNEVL
                     LMYSKTRLKNYEAYNMTIGEGIVISGASRQGTFWGTRTLLQLFENHKNIIPCGLVYDY
                     PNYPSRGFMLDTGRKFFTIDFLRHYVKLLSYYKMNEFQIHLNDNGFKKYFNDDWMQTY
                     AAFRLESESFPGLTAKDGHYSKAEFRNLQLLGMQYGVNVIPEIDAPAHALAFTQYNPN
                     LAASNKAYGMDHLDLYKPEVYEFLDKLYAEYTTGPNPCFVGPDLHIGTDEYNRDESKK
                     FREFTNRYMNYVTQLGKRPRLWGGMRWMNTDTTSVKAQGNAIINAWSRDWVDPYKSLE
                     DGFKLINTCDAMLYIVPAAGYYYDFLNNEWLYNNYRPEKINHNETLPDFTPGVLGGMF
                     AVWNDICGNGISHQDVHYRVYSSLKVIGNKLWKVMPERTYNDFKKLADTTSEGPGLNY
                     SGVYSPEKLKTITSKLGRIAVKFNGNTSLNLGGSDFGYNYDVSFDIKPEPNNNPDAVI
                     FQSSFSKITLNTNGTGKLGFSRDGYVYTFNYIPKNDWQQIRILGDFNSVTLYVDSIRI
                     ERLAPYNINGFNFQQTLFFPLQTAGDKKNGLKGQLKNLSIQYQDLSKIKSAFVASKTT
                     LTTGRYYIKCGEKYLTNSKPNGKGGEPEFLAKNSDPDMLGAQQWDITLIPSTNRYKIA
                     SATDQRYLNEICAFGTNPYNENWNTYNFYKKNNLYAIQNDGNGGTGFWLIDKNRFYTG
                     AESYDDSNYVVEFVPIKD"
     gene            3295..4353
                     /locus_tag="PKH68_07990"
     CDS             3295..4353
                     /locus_tag="PKH68_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNX12916.1"
                     /translation="MFEKETSEAIELLKRMISHPSFSREEKTCADLIEHYIEEEGYTP
                     SRIGNNVWILGSNYDRSKPTLLLNSHIDTVKPVDGWNFDPFLPVENEGKLYGLGSNDA
                     GASVVSLLQVFFLLSKEKQTYNLIFAASAEEEISGSNGMESLLKELPKIDFAIVGEPT
                     NMQLAVAEKGLMVLDCIARGKAGHAARNEGENAIYKALADIEWFKNYQFPKVSEFLGE
                     VKMSVTQINAGTQHNVIPDKCSFVVDIRSNELYNNADILAVINSNVSCEVTARSTRLS
                     SSATPQNHPVVTKAKGMGMDIFGSPTLSDQALMPFASVKIGPGQSSRSHTANEYILTD
                     EIHEGIKKYVELLNGLSL"
BASE COUNT         1499 a          918 c          911 g         1198 t
ORIGIN      
        1 aaaaaaggaa ttccagtcga aaactggcct gaggagctga aagggtggat gaaggaaaga
       61 aaactgatca aataatcaaa ataaaaaagg attttgcagt gaacaaaatc cttttctatt
      121 aaagatactt ctgataatct gattttattt caggggtgtt tcggaagaca aaattatatg
      181 aataaccgta aggaaacacg gacaaacaag cagactaaaa ccacgtaaag aacattctat
      241 gaccttgata taaaaagaaa atttgaattt ctgcttaatt tgtttgaaat gagtgctgat
      301 tttaattgca gcaatttaca aactccattg gcacattgaa ttgcttttca aacagttcaa
      361 acagaatttt ccactaaaat attatctcgg cgacaatgta aatgacatca aaatacatct
      421 tatatcaaga ttttgttttg aagctaaaca aaattccgaa ttttatccga caacattgat
      481 tcaaatctaa ggtaaatttt aattaagtat gcaaattttg attaaatatc tcgccgaaaa
      541 acaatagtct tctgaaagat tatatcctgt aaaatatcct gaagtaaaat taatgattat
      601 gcctgaatat ctatcaaata accacattct gaccaatttt cataaatatg tgttatcttg
      661 gtccgttttt tcatataaaa atcacaaata cttaattcta aatatacttt gaaaaaacaa
      721 tgaaaagaat ctttttaatc aacacgaggt tagttaccat cctactattt gtcagctttt
      781 tattccagca aaatctcaaa gctcaggttt caaaactgaa ggttatacct gaagtacaac
      841 agtttacagc atcatccggt accacatcga tgagccgaaa agtgaaaata ctcgtagaaa
      901 ccgttcactc agacagcctc atgcctattg cactgcaact gaaagatgaa ctgaaaacga
      961 tgatgaatat caccgcttcg attgctgaga caaattcaac aacatcgaaa aaaaatgaag
     1021 tattgctgat gtacagcaaa acccgactga agaattatga agcctataac atgactatcg
     1081 gtgagggtat cgtcatatcg ggagcatcca gacagggtac attctggggc acacgtacat
     1141 tactccagct gttcgaaaat cataaaaata ttatcccatg cggacttgta tacgattatc
     1201 caaactatcc aagccgcggt tttatgctcg ataccggtcg taaatttttc accattgatt
     1261 ttctgcgtca ttatgtgaaa ttgctttcat attacaaaat gaatgaattt cagatacatc
     1321 tcaatgataa tggtttcaaa aaatatttca acgacgactg gatgcagact tacgctgcct
     1381 tccgactcga aagcgaaagt ttcccgggtc tgaccgctaa agacggtcac tattccaaag
     1441 ccgaatttcg taatctgcaa ttgctgggca tgcagtatgg tgtcaatgtc attcctgaaa
     1501 tcgatgctcc ggctcacgcg ctggctttca cccaatacaa tccaaacctg gcagcatcaa
     1561 acaaagctta cggcatggac catctcgatc tttataagcc cgaggtctat gaatttctgg
     1621 ataaactgta cgctgaatac actacaggac ccaatccctg ctttgtagga ccggacctgc
     1681 atatcggaac cgatgaatat aacagagacg aatcaaaaaa attccgggaa tttacaaacc
     1741 gatacatgaa ctatgtcact caacttggaa aacgtccgcg tctgtggggc ggcatgaggt
     1801 ggatgaatac cgacaccact tcggtgaaag cacaagggaa tgcgattata aatgcatgga
     1861 gccgcgattg ggtagatccc tacaagtcgc tcgaggatgg tttcaaactg atcaatacct
     1921 gcgatgcaat gttgtacatt gttcccgcgg caggttatta ctatgatttt cttaataatg
     1981 aatggcttta taacaactac agacccgaaa aaataaacca taatgaaacg cttcctgact
     2041 ttactcccgg agtgctcgga ggtatgtttg cagtctggaa cgatatctgc ggtaacggaa
     2101 tttctcatca ggatgttcac tatcgcgttt acagttcatt gaaagtgata ggaaacaaac
     2161 tttggaaagt catgcctgaa cgcacttata atgacttcaa aaaactggcc gatacgactt
     2221 ctgaaggacc cggactgaat tattcgggtg tatacagccc tgaaaaactg aaaacaatta
     2281 cttcgaaatt aggcaggata gctgttaaat tcaacggtaa caccagtctc aacctgggtg
     2341 gcagtgattt tggttataac tacgatgtgt cgttcgatat caaacctgaa cccaacaaca
     2401 acccggacgc tgtaattttt caaagttctt tttcaaaaat aacgctgaat accaatggta
     2461 ccggtaaact cggattttca cgggacggat atgtttatac ctttaactat attcccaaaa
     2521 acgactggca gcagatccga attctcggtg actttaactc agttacactg tatgttgaca
     2581 gtatccggat tgaaagactg gcaccttaca acataaacgg cttcaacttc cagcaaacct
     2641 tgtttttccc actccagact gccggagata agaaaaacgg cttgaaaggt cagctaaaaa
     2701 acctcagcat ccaatatcag gatctttcaa aaataaaatc agcctttgtg gccagcaaaa
     2761 ccacactgac aacgggacgc tattacatca aatgtggaga aaagtacctt accaactcca
     2821 aaccaaacgg aaaaggcgga gaacccgaat ttctcgctaa aaattcagac cccgatatgc
     2881 ttggcgcaca gcagtgggac ataacgctca taccttctac aaacaggtat aaaatagcca
     2941 gcgccacaga ccagagatac ctgaacgaaa tttgcgcgtt cggaactaat ccgtataatg
     3001 aaaactggaa cacgtataat ttctacaaaa aaaataatct gtatgccatt cagaatgatg
     3061 gtaacggcgg aacgggtttc tggttaatag ataaaaatcg tttttataca ggtgcagaat
     3121 cctacgacga ttcgaattac gtggttgaat ttgtgccgat taaagattaa aaccaatgca
     3181 ataaatacta tccggttcaa catatgtcag tttaaaatgt tatttaatgt gacaggcatt
     3241 tgatacaaaa agcgggaaat ccggttacta ataattagac agtttaatat cattatgttt
     3301 gaaaaagaaa catccgaagc aattgagctg ctgaaaagaa tgatttcaca tccttcgttc
     3361 agccgtgagg agaaaacatg cgccgatctg attgaacatt acatcgaaga ggagggatat
     3421 accccttcgc ggattggtaa taatgtctgg attttgggaa gtaattatga cagatcgaag
     3481 cccacgctgc tgctcaattc gcatattgat accgtaaaac cggttgacgg atggaatttt
     3541 gatccttttt tgccggtgga aaacgaggga aaactatacg ggctgggaag caacgatgca
     3601 ggcgcttcgg tggtttcgct gttgcaggtt tttttcctgt tatcgaaaga aaaacagaca
     3661 tacaacctga tattcgcggc atcggcagag gaggaaatct ctggcagtaa cggtatggaa
     3721 agtttgctga aagaacttcc gaagattgat tttgccattg tgggtgagcc taccaatatg
     3781 cagttggcag tggcagaaaa aggactgatg gtgctggatt gcatcgcgcg gggcaaggct
     3841 ggacatgcag cccgtaacga aggagaaaac gcgatttaca aagcgcttgc agacatagaa
     3901 tggtttaaaa attatcagtt cccgaaggtt tctgaatttc tgggtgaggt gaaaatgagc
     3961 gtaacgcaga taaacgcggg aacacagcac aatgtaattc ccgacaaatg cagttttgtg
     4021 gtggatatcc gcagcaacga gctttacaat aacgccgata ttctggctgt aatcaactca
     4081 aacgtcagct gtgaggtaac ggcgcgttcc acccggttga gttcgtcagc gacgccgcaa
     4141 aatcatcctg tggttacgaa agccaaaggg atgggaatgg atatcttcgg ttcccccacg
     4201 ctttcggatc aggcactgat gcctttcgca agtgtgaaaa taggtccggg acagtcatca
     4261 cgctcacata cagcgaatga atatattctg actgatgaaa ttcacgaagg aataaaaaaa
     4321 tacgttgaac tgctgaacgg tttatcgttg taacaccgga gataatacaa acaaaaagag
     4381 aagaccgaaa atcttctctt tttttgcctt ctattccact tttccccgga atgaaatgga
     4441 ctggcgggaa gtatccataa catccagatc tgcgtttcca ttttcccaga tattcagcaa
     4501 taacaaaatt tcgtttcgct gatcgc
//
LOCUS       DAOPZK010000149         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_24832_length_4504_cov_22.651158, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000149 DAOPZK010000000
VERSION     DAOPZK010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24832_length_4504_cov_22.651158"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /gene="rlmD"
                     /locus_tag="PKH68_07995"
     CDS             <1..1292
                     /gene="rlmD"
                     /locus_tag="PKH68_07995"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNX12917.1"
                     /translation="GDVVNLQVTRKKSSFMEARVTELVQASETRQEPFCEHFGICGGC
                     KWQILPYSEQLNYKQKEVENNLRRIGKVELPEISPIKGSAKTIFYRNKLEFTFSNRRW
                     RTFEEMNAAKEFDTMNAVGFHIPGMFDKVLDINKCWLQDDIQNQIRNEIRSYALQNNL
                     TFFDLRVQEGFLRTMMVRTTSTGELMIILVFFHEDKTEREKLLQHITDKFPEITSLLY
                     VINSKANDTITDQEVLTFKGNDSIYEEMEGLRFKIGPKSFYQTNSEQAYELYKVARNF
                     AELSGNEVVYDLYTGTGTIANFVARNCSKVIGIEYVPEAIDDAKENSALNGIENTLFF
                     AGDMKNVLNAEFIRTHGKPDVIITDPPRAGMHEDVVKAILAAEPERIVYVSCNAATQA
                     RDLSLLDEKYRVTAVQPVDMFPHTHHVENVVKLEKRI"
     gene            1494..2213
                     /locus_tag="PKH68_08000"
     CDS             1494..2213
                     /locus_tag="PKH68_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX12918.1"
                     /translation="MNLLSQLLEEKMMGLKGGLYHQTQIRLAFNSNRIEGSRLSEEQT
                     RYIYETNTLHLENGDSTANVNDIIETVNHFLCFDYMLDVAREPLSEIQIKEFHRLLKN
                     GTSDARKTWFRVGEYKSKPNVVGGIETSLPKDVHTDMRNLLNQYHQKESVTLQDIVDF
                     HYHFEAIHPFQDGNGRVGRLIIFKECLANNILPFIIEDENKFFYYRGLKEYPRVKEFL
                     TDTCLSAQDDYLKMVEYFTNK"
     gene            2230..3855
                     /gene="nadB"
                     /locus_tag="PKH68_08005"
     CDS             2230..3855
                     /gene="nadB"
                     /locus_tag="PKH68_08005"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNX12919.1"
                     /translation="MARKYDFVVVGGGIAGISFALKAAQYGKVALLCKTTLEESNTSY
                     AQGGVATVMYEPDNYEKHIEDTLIAGDGHCDKEAVELVVKNARAQIDQLLEWGMDFDK
                     TDDGSKFDLHKEGGHSEFRILHHKDNTGFVIQESLNKQIRNHPEIELYEHYFAIEILT
                     QHHLGEIVTQHTPNIECYGVYALNQKSNHIHTFLSKVTVLATGGIGSVYQASTNPAIA
                     TGDGVAMVHRARGAIKDMEFVQFHPTALYHPGDKPVFLITEAIRGYGAVLRGRDGREF
                     MQKYDSRGSLAPRDIVARAIDNEMKIHGDSFEYLDVTHKNPEETKEHFPMIYHKCLEY
                     GIDITKDYIPICPTQHYMCGGIAVDLNGQSSINRLYAIGECSATGLHGANRLASNSLI
                     EAIVYADVAVKHAVPRLDKLTINEMLPAWNDEGTSLTEEMVLITQSFREVEQIMSTYV
                     GIVRSNLRLQRAMSRLEILFRETESLFDRSVVSREICELRNVISVSYLIIKHAIARKE
                     SRGLHYTVDYPRKMEEERNNAELIKKKKKSDTK"
     gene            3901..4206
                     /locus_tag="PKH68_08010"
     CDS             3901..4206
                     /locus_tag="PKH68_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNX12920.1"
                     /translation="MIKHIVCFRLADEAEGKTKAENAQIIKEGLENLINLIPQLRKIE
                     VGINHPATPATNFDIVLYSEFDSIDDLNTYQEHPEHKKVGAYIVKVKTDRVCVDYEV"
BASE COUNT         1397 a          960 c         1060 g         1087 t
ORIGIN      
        1 cgggcgatgt ggtgaatttg caggtgacac gtaagaaaag cagctttatg gaagctcgcg
       61 taactgaact ggtacaggct tcagaaacca ggcaggaacc gttttgcgaa catttcggca
      121 tctgcggtgg atgcaaatgg cagattttac cctattccga acaactcaac tataaacaaa
      181 aggaggttga aaataatcta cgacgaatag ggaaagtgga actgcccgaa atctccccga
      241 ttaaaggttc agctaaaacc attttttacc gcaacaaact ggaattcact ttctcaaacc
      301 gccgctggcg cacttttgag gagatgaatg cggctaagga attcgacacg atgaatgcag
      361 ttggtttcca tattccgggt atgttcgata aagtgctgga tatcaacaag tgctggctgc
      421 aggacgatat ccaaaaccaa atccggaacg aaatccgcag ttatgccttg cagaataacc
      481 tgacattctt cgatttacgt gtgcaggaag gttttttgcg caccatgatg gttcgcacca
      541 catccaccgg tgagctgatg attattctgg tgtttttcca cgaggacaaa accgaaaggg
      601 aaaaattgct gcaacatatt actgataaat ttccggaaat aacctcgtta ttatatgtga
      661 ttaactccaa agccaacgac accattacag accaggaagt gttgactttc aaaggcaacg
      721 atagcattta tgaagaaatg gaagggctgc gcttcaaaat cggtccgaaa tcattctacc
      781 aaaccaattc agaacaggca tacgaactgt ataaggttgc ccgcaatttt gcagaattgt
      841 ccggcaacga agtggtttac gatctctaca ccggcaccgg caccatagcc aatttcgtgg
      901 ctcgcaactg cagcaaagtg atagggatag agtatgtacc cgaagccatc gacgatgcaa
      961 aagaaaattc agctctcaac ggaattgaaa acaccctgtt ttttgccgga gatatgaaaa
     1021 atgtgctgaa tgcggaattt attcgtactc acggtaagcc tgatgtaatt attaccgatc
     1081 caccgagggc cggcatgcac gaagatgtgg tgaaagccat actggcagcc gaacccgaac
     1141 gaattgtgta tgtgagttgt aatgcggcca cacaggcgcg cgatttgagt ttgctcgacg
     1201 aaaaataccg cgttacggcc gtgcagccgg tggatatgtt tccgcataca catcatgtgg
     1261 agaatgtggt gaagttggag aagaggattt aattgccagc ccactaaccc cagcctctcc
     1321 ctaaatccct ccccaaaagg gagggacttt taaaagaggg ggaatgagag tgagatattt
     1381 tattgaagag aaatgattac gtgagtaaaa taaaaatatt tattacatct cagttattgg
     1441 tttttattat ttttgtagag aacgaaatta ttaactcatt ttaaaaaaca taaatgaacc
     1501 tgttatccca actgctggaa gaaaaaatga tgggtctgaa aggtggtttg tatcatcaga
     1561 cacaaatcag gctggctttc aattcgaaca ggattgaagg cagccggctt tcggaagaac
     1621 agacgcgtta tatctacgaa accaacacgc tccacctcga aaatggcgat agtacagcaa
     1681 atgtgaacga tattattgaa actgtaaatc acttcctttg ttttgattat atgctcgatg
     1741 tagccagaga accattatcc gaaattcaaa tcaaagagtt tcatagatta ttaaaaaatg
     1801 gtacatctga tgcccggaaa acatggtttc gggtgggcga atataaatct aaaccaaatg
     1861 tggtaggcgg aatagaaacg tcgctcccaa aagatgtaca tactgatatg aggaatttat
     1921 taaatcaata tcatcaaaag gaaagtgtta ccttgcaaga tattgtagat tttcattatc
     1981 attttgaagc cattcatcca tttcaggatg gaaatggaag agtggggagg ctgattattt
     2041 tcaaagaatg cctggcaaac aacatattgc cttttattat tgaagatgag aataaatttt
     2101 tctattacag aggcttgaaa gaatatccac gagtgaaaga atttctcaca gatacatgcc
     2161 tttcggcgca ggatgattac ctgaaaatgg tagaatattt tacaaataaa tgaaaggaga
     2221 ctaaatatta tggccagaaa atacgatttt gtagtggttg gtggcggtat agccggcatc
     2281 agctttgcgc tgaaagctgc tcagtacggc aaagtggcgc tgctttgcaa aactaccctc
     2341 gaagaaagca acacctccta cgcgcagggt ggggtggcta ctgtgatgta tgagccggat
     2401 aattacgaga aacacattga ggataccctc attgccggcg atggacattg tgataaagaa
     2461 gcggtggaac tggtggtgaa aaatgcacgg gcacagattg atcagttgct ggagtgggga
     2521 atggatttcg ataaaaccga cgatggatca aagttcgacc tgcataagga aggcgggcat
     2581 tccgaatttc gtattctgca tcacaaggat aataccggat ttgtcattca ggaaagcctg
     2641 aacaaacaga tacgcaatca tcccgaaatt gagttgtacg agcattattt tgccattgaa
     2701 attctcacac agcatcacct gggcgaaatt gtaacccaac acacacccaa cattgaatgc
     2761 tacggggtgt atgccctcaa tcagaagagc aaccatattc atacatttct gtctaaagta
     2821 accgttctgg ctacgggtgg cattggcagt gtgtatcagg catccaccaa tccggccatt
     2881 gcaaccggcg acggcgtagc catggttcat cgtgcgcggg gggccattaa agatatggaa
     2941 tttgtgcagt ttcaccccac agcactctat catccgggcg acaaaccggt atttctcatc
     3001 accgaagcca ttcgcggtta cggtgccgta cttcgcggcc gcgacggccg ggaatttatg
     3061 cagaaatacg acagtcgcgg ctctctggct ccccgtgata tagtggcaag ggccattgac
     3121 aacgaaatga aaattcacgg tgacagcttt gaatacctgg atgtgacgca caaaaacccg
     3181 gaggaaacga aagagcattt ccccatgatt tatcacaaat gcctggaata tggcatcgat
     3241 attaccaaag attatattcc catttgcccc acgcagcact atatgtgcgg cggtatagcc
     3301 gtggatctca acggtcagtc gtccatcaac cggctttatg ccattggtga atgttcggcc
     3361 accggactgc acggagccaa ccggctggca tccaattcgc tgatagaagc catcgtgtat
     3421 gccgatgtgg cggtgaaaca tgctgtaccc aggctggata aactcaccat aaacgaaatg
     3481 ctccccgcat ggaacgatga aggcacatcg ctcaccgaag aaatggtatt gataacccaa
     3541 agttttcgcg aagtggagca aatcatgagt acctatgtgg gcattgtacg ctccaacctc
     3601 cgtttgcagc gtgccatgag ccgcctggaa atccttttcc gggaaacaga aagcctcttc
     3661 gaccgttcgg tggtttcgcg ggaaatatgc gaactgcgca acgtcatcag cgtgtcgtac
     3721 ctcataatca aacacgccat tgcccgcaag gaaagccgcg gactgcatta cacggtagat
     3781 tatccccgaa aaatggagga agaaagaaat aatgctgaat taataaaaaa gaaaaagaaa
     3841 tcagatacta aataaatact aattaatttt ctccccgttg agggaggtta ggaggggctt
     3901 atgatcaaac acattgtatg tttccggctg gcagacgaag ccgaaggaaa aacgaaagca
     3961 gaaaatgctc agataattaa agaaggactg gaaaacctga ttaatcttat tccgcagctc
     4021 agaaaaattg aagtggggat aaatcatccg gctacgcccg ctaccaattt tgatatcgtg
     4081 ctgtacagcg aattcgacag catcgacgac ctgaacacct atcaggaaca tcccgaacac
     4141 aaaaaagtgg gcgcgtacat cgttaaggtg aaaaccgacc gggtttgtgt ggattatgaa
     4201 gtgtaaaaag ctgtaaacta caaactccaa actgtcgtag ggtagcgtag gaatattgaa
     4261 ataaatgcct gattttcaat gacgaaaatc aggcatttat tattcacctg agcggaggac
     4321 aagaatcgaa cccgctcttg cgtaccggag gtacgccgtc aggctctgaa ggcctgtgtg
     4381 ctgccattac accacttccg caaaaagaaa aacctaccgg gtgaagcggt tttcagccgt
     4441 gtttatttta cggcggttac aaaccaccgc accccgggaa ctgtaaaaat atgtcgtatg
     4501 tgga
//
LOCUS       DAOPZK010000150         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_25011_length_4483_cov_26.218835, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000150 DAOPZK010000000
VERSION     DAOPZK010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25011_length_4483_cov_26.218835"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..1046
                     /locus_tag="PKH68_08015"
     CDS             375..1046
                     /locus_tag="PKH68_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNX12921.1"
                     /translation="MQPTQEYMNFYPKNKLPVREVKKFVLIFYLVGLLGFLLPFSKNI
                     FIAITPFALLLSTYLAAIYHEDYTRKSVLIFLLIFTLGYFIEVIGVNTGLIFGQYAYG
                     KGLGIKLLNTPLMIGVNWLFLTYTSISIVQKLKLKQFLTILLAPLLMVLYDIILEQLA
                     PVMDMWSWQGAVVPLRNYVAWYLFAVVFVAIFRIFRIDTRNPLAGILFYCQFLFFVLL
                     KLLIR"
     gene            1051..1671
                     /locus_tag="PKH68_08020"
     CDS             1051..1671
                     /locus_tag="PKH68_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX12922.1"
                     /translation="MIKARHHAVIYPIFRILTRYLMSHKFHSVHIEGDFEDTGKPVLV
                     IANHVSWWDGFWILYLNIKVLRRRFHFMMLEEQLKKHWYFQYIGGYSVKKKTRSIVES
                     IGYTIELLRDNGNMVFMFPQGEITSMHNHAIVFEKGAQRIVDGSSDDVQVLFMANFID
                     YFSDPKPNVFMYLQKFPAQGLKKNQLEFHYNKFYQQALNTQKIKVS"
     gene            1671..2765
                     /locus_tag="PKH68_08025"
     CDS             1671..2765
                     /locus_tag="PKH68_08025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX12923.1"
                     /translation="MQYAAYISLAFLAMQLVNVLLNFIFSQKIKKPTAYNDELISVLI
                     PARNEEANIGALLQSLCEMKNARLEILVCDDESTDRTSAIVSEYSKKNPAIKLLKSDG
                     LPAGWLGKNHACYQLAQHAAGAYYLFIDADVKLQGTVITDTVSYLKKYRLGLLSIFPT
                     QIMKTFGEKITVPVMNYILLTLLPLIFVRISPFSSHSAANGQFMLFDAGVYKKFEPHQ
                     HFKSSAVEDISISRFFKKNKTKIACITGEKRVECRMYTSYEAAINGFSKNIFMFFGNN
                     PVLAFVFWTLSTLGFVPVIIWKWQYFPVYLLALLLIQLIYSAAGRQNPLTNIAFFPLQ
                     LGFLLQVMVKSLSLKKQKQSTWKGRNISTY"
     gene            2735..3469
                     /locus_tag="PKH68_08030"
     CDS             2735..3469
                     /locus_tag="PKH68_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12924.1"
                     /translation="MERKKYFYILIFCLLALNSTAQNHKSIYKAYISGDMAKWKKTID
                     SIEALPDKSNPVKLDLLNYQYGYIGWCISKDKKTEAEKFIKKSKELMRQLEQNKYSIA
                     VLDAYKAAFIGFEIGMSPYKAPFIGKQSLIFAKKSVEADAQNTLGYVQLGNIAFYTPK
                     TFGGNKSDAMQHYLKALKIMDSKNEQKKHNWNYLNLLATIINAYMEVGDYESARKYCV
                     RTLAAEPEFDWVKNQLYPQVLKKLKP"
     gene            3466..>4483
                     /locus_tag="PKH68_08035"
     CDS             3466..>4483
                     /locus_tag="PKH68_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNX12925.1"
                     /translation="MSKKVLIVGTGLGGLSTGLRLAARGYEVAFVEKATQPGGRLNRL
                     KKDGFTFDVGPSFFSMPYEFEELVKDCGIEMPFEFVELDPLYTVRFRGSDRKFSLYKD
                     IDKMSAQFEGIEPDFKAKFTAYLKKCGELYNDTVGIVIKQNFDSIPDYLKSLMRVNPK
                     HLPVLLKTFWQQTNSFFTSKEARQIVSLIAFFLGRTPFDTNAVYTLLSHIEFSHTGYY
                     NVKGGMYTIVESLVEELKKHQVTFNYETEIVDFTSENNKLTAVTDQNGKAWTADIFII
                     NADAAFFRGSIFKRKEFSDNKLKKMSWTMGYLTFYVGVKAQLPQVEHHNYFLGDNYED
                     YARDV"
BASE COUNT         1353 a          916 c          924 g         1290 t
ORIGIN      
        1 ttctcactac gttcgaaatg acagtcagta ttttatcttt aaggaggggg gttggttggc
       61 ggctaagccg ccaaccaacc cccctcctta cctaaaacgc cataattccc tgtcatccag
      121 agcgaagcga tggatctaaa tatttttacc ggacaacaat gattttttta ctacgaacca
      181 gcctcgttta tcccgacttg tcgggacgga tagcaggtaa cactaattgc gcgaaaaaaa
      241 ttagtttcat tcgtgatatt agtagttaaa atttaaaaca acagagtgtc ttttatcttt
      301 taatatgaat tctgagttga aataaataag tcgagacgaa ctctcaatat aaatcctttg
      361 cattaaccca caacatgcaa ccaacacagg aatacatgaa tttttatcca aaaaataaat
      421 tacccgttcg ggaagttaag aaatttgtac tgatttttta cctggtgggt ttattgggct
      481 ttttattgcc tttcagtaaa aatattttca ttgccataac gccgttcgcg ttgctgctaa
      541 gcacttacct ggcagccatt tatcacgaag actacacccg aaaatcggtt ctgatatttt
      601 tgctgatttt tacgctgggt tattttatcg aagttatcgg tgtgaatacc ggtctcatat
      661 tcggacagta tgcctatggc aaaggactgg gtatcaaact tctgaacaca ccgctgatga
      721 ttggggttaa ctggctattt ctaacctata cttcaatttc gattgtacaa aaattgaagt
      781 taaaacaatt tcttacaatc ctgctggctc cgctgctgat ggtattgtac gatattattc
      841 tggagcaatt ggcacccgtc atggatatgt ggtcgtggca gggagcggtc gttccactcc
      901 ggaattatgt agcctggtat ctcttcgctg tggttttcgt agcaattttc aggatattca
      961 ggattgacac ccgaaatccg ttggcaggca tcttgtttta ttgtcagttt ttattttttg
     1021 tattgttgaa attattaatc cgctgatcac atgattaaag ccagacacca tgcggtcata
     1081 tatcccattt tcagaatact tacgcggtat ctgatgagcc ataaatttca ttcggtacat
     1141 attgaaggag attttgagga cacaggaaaa ccggttcttg tcattgccaa tcatgtgagc
     1201 tggtgggatg gtttctggat tttatatttg aatatcaagg ttctacgtcg caggtttcac
     1261 tttatgatgt tggaagaaca attaaaaaaa cactggtact ttcagtatat aggtggatat
     1321 tcggtgaaaa agaaaacgcg tagtattgtg gagagtattg gctataccat tgaattactg
     1381 agagataacg ggaacatggt ttttatgttt ccacagggag aaattacttc gatgcataat
     1441 catgccattg ttttcgaaaa aggtgctcaa cgcatcgtcg atgggagttc cgatgatgtg
     1501 caggtgcttt tcatggctaa tttcatcgat tatttttccg atccgaagcc gaacgttttc
     1561 atgtaccttc agaaatttcc ggctcaggga cttaaaaaaa atcaacttga attccattac
     1621 aataaatttt accaacaggc tctcaacact caaaaaataa aagtatcctg atgcaatatg
     1681 cggcatacat atcacttgct tttttggcca tgcaattggt caatgtgctg ttgaacttta
     1741 tttttagcca gaaaataaaa aaaccgactg catacaacga tgaactcatc tcggtattga
     1801 taccggcacg caacgaagaa gcaaatattg gcgcactgct gcaaagtctg tgtgaaatga
     1861 aaaatgcccg gctggaaatc ctggtctgtg atgatgagtc aaccgaccgc acctccgcca
     1921 tagtttccga atattcaaaa aaaaatccgg ctattaaatt attaaaatca gacggattac
     1981 ctgccggctg gctgggtaaa aaccatgcct gctaccaatt agcccaacat gctgcgggtg
     2041 catattatct ttttattgat gctgatgtga aactgcaggg cactgttatc actgatacgg
     2101 tatcgtacct gaagaaatac cggttgggat tgctgtccat tttccctaca caaattatga
     2161 aaacgttcgg cgaaaaaatc accgtaccgg tgatgaacta tattttactc actctcctgc
     2221 ccctgatatt tgtccggatt tcgccattca gttcacactc agcggccaac ggacagttta
     2281 tgctcttcga tgctggtgtg tataaaaaat ttgaacctca ccaacacttt aaaagctcgg
     2341 cggtggaaga catttcgatt tcccggtttt tcaaaaaaaa taaaacaaaa atagcatgca
     2401 tcaccggtga aaaacgagtg gaatgccgga tgtacacctc atacgaggcg gcaataaacg
     2461 gtttttcaaa aaatatcttt atgttttttg gcaacaatcc cgtcctggca tttgttttct
     2521 ggacattatc cacacttgga tttgtgccgg ttatcatctg gaaatggcaa tattttcctg
     2581 tttatcttct tgccctgctg cttattcagt taatttactc tgctgcgggc aggcaaaatc
     2641 ccctgacgaa tatagctttt ttcccgctgc agctgggttt cctgctacag gtgatggtga
     2701 aaagtctttc attaaaaaaa caaaaacaat ccacatggaa aggaagaaat atttctacat
     2761 attgatattc tgtctgttgg cacttaattc cactgctcag aaccataaat ccatttataa
     2821 ggcttacatt agtggtgata tggcaaaatg gaaaaaaacc attgattcga ttgaagcgct
     2881 tccggataaa agcaatcctg taaaacttga tttgcttaat tatcaatacg gctatatcgg
     2941 gtggtgtatc agcaaggata aaaaaaccga ggcagagaaa tttattaaga aatcgaaaga
     3001 gttgatgcgg cagctggagc aaaacaaata cagcatcgca gttttggatg cttacaaagc
     3061 cgcatttatc ggatttgaaa tcggcatgtc gccttataaa gccccgttta ttggtaagca
     3121 gagtctgatt tttgccaaaa aatcagttga agcagatgca caaaacacac tcgggtacgt
     3181 tcagttgggc aacatcgcct tttatacacc caaaacattc ggcggcaata aatccgatgc
     3241 catgcagcat tatctgaagg ccttaaaaat aatggacagc aaaaatgagc agaaaaaaca
     3301 caactggaat tacctcaatc tgctggctac cattatcaat gcctatatgg aagtgggcga
     3361 ttacgaatcg gcccggaaat attgcgtcag aacactggcc gctgagcctg aattcgattg
     3421 ggtcaaaaat cagctttatc cacaagtatt aaaaaaatta aaaccatgag taaaaaagta
     3481 ttgatagtag gaaccggcct gggtggtttg tccaccggcc tgagattggc tgccagaggt
     3541 tacgaagttg catttgtaga aaaagcaacg caacccggag gtcggctgaa ccgattgaaa
     3601 aaagacggat ttacatttga tgtgggaccg agttttttca gtatgcccta cgagttcgaa
     3661 gaactggtga aagattgcgg tattgaaatg ccctttgagt ttgtggaact cgacccgctt
     3721 tacaccgtac gattccgtgg cagcgaccgg aaattctcgc tgtacaaaga tattgacaaa
     3781 atgagcgctc agttcgaggg aatagaaccg gatttcaaag ctaaattcac tgcttatctg
     3841 aaaaaatgcg gtgaactgta caacgacacg gtgggcattg tcatcaagca gaatttcgac
     3901 agcatacccg attacctcaa atcactgatg cgcgttaatc ccaaacattt acccgtatta
     3961 ctcaaaacct tctggcagca aaccaactca tttttcacgt cgaaggaagc ccggcaaatt
     4021 gtgtcgctca ttgctttttt cctgggtcgt accccgttcg ataccaatgc cgtttatacc
     4081 ttactttcgc acatcgaatt ttcacatacc ggctactaca acgtaaaggg aggcatgtac
     4141 accattgtgg aaagtctggt ggaagaactg aaaaaacacc aggtaacgtt taactatgaa
     4201 actgaaattg tagattttac ctccgaaaac aataaattaa cggcagtcac tgatcaaaac
     4261 gggaaagcct ggaccgctga cattttcatc atcaatgccg atgcagcttt tttcagaggc
     4321 agtattttca aacggaaaga attttcggat aataaactga aaaaaatgag ctggacaatg
     4381 ggttatctga ctttctatgt aggagttaaa gctcagctgc cacaggtgga acatcacaat
     4441 tacttcctgg gcgacaatta cgaagattac gcccgcgatg tga
//
LOCUS       DAOPZK010000151         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_25240_length_4454_cov_31.209820, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000151 DAOPZK010000000
VERSION     DAOPZK010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25240_length_4454_cov_31.209820"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PKH68_08040"
     CDS             complement(<1..479)
                     /locus_tag="PKH68_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNX12926.1"
                     /translation="MNKNFNGKRVSQTVMFFLFRLMSYVVVGILFSIIGFIVIKGIGV
                     INWEFLTTAPEDGMTKGGIFPAIVGTFYLVVGSMLVAFPIGVMSGIYMSEYASNKHIK
                     RIIRVMTNNLGGIPSIVFGLFGMALFVNRLNFGDSIIAGSFTLGLLALPLIIRVTEEA
                     "
     gene            complement(523..1728)
                     /gene="pstC"
                     /locus_tag="PKH68_08045"
     CDS             complement(523..1728)
                     /gene="pstC"
                     /locus_tag="PKH68_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNX12927.1"
                     /translation="MFKRKKNRFIETIVETLLRFSGGITTLTILLISIFLFTEGWGLF
                     KSSTVEKGYILCVNKSNPVKNLTPTQIKEIFDGNITSWKQVGGSDIPIVPLRMDDVAS
                     MYTEEEIGAEFEHLPAKLSEAIEKEPGALGFFPEQFIDIKFKGRVLPAETITLKEYFT
                     GKEWFPTATPASQFGLLPLLLGTLWVSLGAILLALPFGMAVAIYLAEIANNRVRKILK
                     PVIELLAGIPSVVYGFFGLIVIVPLIRSLFNLPVGETALAGSIVLAIMALPTIITVAE
                     DAMRTTPLAMKESSLALGATHWQTIYKVIIPYASSGIMAAIVLGIGRAIGETMAVLMV
                     TGNAAVIPHTLTEPVRTIPATIAAELGEASMGSVQYQALFMLGAILFVLTLGISIWAE
                     IIRKEPQSD"
     gene            complement(1856..2671)
                     /locus_tag="PKH68_08050"
     CDS             complement(1856..2671)
                     /locus_tag="PKH68_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNX12928.1"
                     /translation="MKRFFLSLAMIAIIASMTMAQKIKGSDTVLPLAQKEAEAYMKNN
                     PSAKLTVTGGGSGIGFAALIEGTTDIAMASRSIKFDEKMKMQNGGKTAKQVVIAKDAL
                     TVIVHPSNKVTKLTREQLESIFTGKITNWKQVGGADLAITAYSRETTSGTYEFFKEHV
                     LKNKNYKSGILSMPATGAIIQSISQTPGAIGYVGMAYVTKNVKEIWVSFDGGKNYIEP
                     TVANSKAGKYPIVRPLYFYYDTKKEASVKPFVNFVLSAEGQQLIDKVGYLSIK"
     assembly_gap    2806..2905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2906..3501)
                     /locus_tag="PKH68_08055"
     CDS             complement(<2906..3501)
                     /locus_tag="PKH68_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12929.1"
                     /translation="MKLKSILYTVAIATSVFTATAQDENLTPLEQVQQKVETHDVTIS
                     KLNNLKITGYIQPQYQWGEESAALRVGTANTDATKSFNRIGIRRGRLKFTYDAGELAS
                     GVFQLNIIDKPGLSGATVQLKEAYLNVKAPWLKTSALRAGIFDRPFGNEISYSSSLLE
                     SPERSRIVNQLFPEETDLGGMLVLQPAATSSFNFLKFEG"
     gene            complement(3789..>4454)
                     /locus_tag="PKH68_08060"
     CDS             complement(3789..>4454)
                     /locus_tag="PKH68_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX12930.1"
                     /translation="KLTQQVAHELKTPLSSIIGYMETLYNNPEITPERRDFFIKRSHS
                     QAVRLNQLLQDVLLLNQMNEAPHSIEMEPVCINKIVENVTDDLDIILHEKNITVDTSF
                     GGDVWLRGNQMLLYSIFRNLMDNSIAYGGDKVKIFIALTGEDAKYFFFSFSDNGMGVG
                     PEHLSSLFDRFYRVDKGRSRKTGGTGLGLSIVKNAVETHKGTISVNNKPGGGLEFVFS
                     LHK"
BASE COUNT         1233 a          915 c          864 g         1342 t
ORIGIN      
        1 gcttcttccg tcacccggat aatcagcgga agcgccagta atccaagtgt aaacgatccg
       61 gctatgattg aatctccaaa attcaatctg ttgacaaaca atgccatccc gaataatccg
      121 aaaacaatgg atggaattcc tcccaggttg ttagtcataa ctcgtatgat gcgcttaata
      181 tgtttgttgg atgcatattc gctcatgtat attcccgaca tgacaccgat ggggaatgct
      241 acaagcatac tgcccacaac cagataaaaa gttccaacga tggcaggaaa aattccgcct
      301 tttgtcatgc catcttccgg tgcggtggta agaaactccc agtttatgac acctatacct
      361 tttataacaa taaaccctat gatagaaaag aggataccca ctaccacgta actcattagt
      421 ctgaaaagga aaaacatgac agtttgagaa actctttttc cgttaaaatt tttattcata
      481 cgactttttt atttttactc agtatgtact gaacactaat tgttaatccg actgaggttc
      541 tttacggatg atctcagccc aaatacttat acccagtgtg agtacaaaca atatggctcc
      601 caacatgaat aatgcctggt attgcacact ccccatagaa gcttcaccca gttcagcggc
      661 gattgttgcc gggatggtac gaacaggttc agtaagcgta tgtggaatta cagctgcatt
      721 cccggtcacc atcagcacag ccattgtttc tcctattgca cgtccgatgc ccaaaacaat
      781 tgcagccatt atacccgatg aggcataagg aattatcacc ttgtaaatgg tttgccagtg
      841 agtggcaccc agtgcaagac tggactcctt cattgctaac ggtgtggtac gcatggcatc
      901 ttcggctacc gtaatgatgg ttggtaaagc cataatggca agcacaatac ttcctgcaag
      961 tgccgtttca cccaccggaa gattgaaaag tgaacgaatt aacggaacaa taacgatgag
     1021 accgaaaaaa ccgtaaacaa ccgaagggat gcccgctaaa agttcgatga caggtttaag
     1081 aattttcctg accctgttgt tggcaatttc tgccagatat attgcaacag ccatcccgaa
     1141 aggtaatgcc agcaagatag cgccaagact tacccatagt gtgcctagta acaatggaag
     1201 taatccaaac tgggatgccg gagttgctgt cggaaaccac tcctttcccg tgaaatactc
     1261 tttcagcgtg attgtttctg caggcaaaac ccgtcctttg aacttaatgt cgataaactg
     1321 ctcgggaaag aagcccaacg cgcccggttc cttttctatt gcctcgctga gttttgccgg
     1381 aagatgttca aattccgctc ctatttcctc ttcggtatac atggaagcta catcgtccat
     1441 acgcaaaggt acgatcggga tgtcgctgcc acccacttgc ttccaggagg tgatgttgcc
     1501 gtcaaatatc tcctttatct gtgtcggtgt cagattctta accggattac tcttatttac
     1561 gcaaagaatg tatccttttt ctaccgtcga cgacttgaac agcccccagc cttcggtaaa
     1621 taaaaagata cttattaata atatagtcag tgttgttatc cctccgctaa agcgcaacag
     1681 ggtttcgaca atggtttcaa taaatctgtt tttttttctc ttaaacacgt tatttacgat
     1741 gtgttagttg tgtaatcttt atatgtactg acagtgattg ttctgagaaa tatataagat
     1801 gaaatggctt tttcacacaa aaaaaccatt tcatctcgtt tataaacaga atttcttatt
     1861 ttattgacaa ataacctact ttatcaatta attgttgtcc ctcagctgag aggacaaagt
     1921 ttacaaatgg tttcacgctt gcttctttct tggtatcgta ataaaaatac agcggtctta
     1981 ctatgggata tttgcctgcc ttgctattgg ctacagtagg ttcaatataa tttttaccac
     2041 catcgaaaga tacccagatt tctttcacat ttttggttac atacgccata ccaacatatc
     2101 ctattgcacc tggagtctgg ctgattgact gaatgattgc accggtagcg ggcatactca
     2161 gaataccgga tttatagttt ttgtttttca atacatgttc tttgaagaat tcgtaagtac
     2221 cggatgttgt ttcgcgtgaa taagctgtaa tcgctaaatc agcgcctccc acctgtttcc
     2281 agttcgttat cttacctgtg aagatacttt caagctgttc gcgtgtgagt tttgttactt
     2341 tattggaagg atgcacaatt acagtaagtg catctttggc tataaccact tgttttgcgg
     2401 tttttccacc gttttgcatt ttcattttct catcaaactt tatactgcgc gaagccatag
     2461 ctatatcagt agtaccttcg ataagtgctg caaagccgat acccgaacca ccgccggtta
     2521 ctgttaactt agccgaagga ttattcttca tatatgcttc tgcttctttt tgggctaaag
     2581 ggagtactgt gtcggagcct ttaatttttt gtgccatcgt catggaagca attatggcaa
     2641 tcattgcaag tgatagaaaa aatcttttca taattttcat atttttataa tgttgttatc
     2701 cgattaataa tcttttgttt ttttttgtta gtgcaaaatt aatttcctga tattacaatc
     2761 ccgttacaga cttgtgaagt tcggattaat atcaaggggt gaaaannnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnccttc gaatttcagg aaattgaacg aagatgtcgc
     2941 ggcaggttgc agtaccagca taccccctaa atcggtttct tcggggaata actgattgac
     3001 tatgcgtgac cgctcgggcg attcgagaag agaggaagaa tatgaaattt cgtttccaaa
     3061 cggacgatcg aaaataccgg ctctcagtgc tgaggttttt aaccatggag cttttacgtt
     3121 aagataagcc tctttgagtt gtacggttgc tcctgatagc cccggtttgt cgatgatatt
     3181 caactgaaat actcctgaag ccagttcacc tgcatcatag gtaaatttca aacgtccgcg
     3241 acgaatacct atacgattga aagattttgt agcatctgta ttggccgtac ccactctcag
     3301 agcagccgat tcttcgcccc actgatattg tggctgaatg tagccggtaa ttttcagatt
     3361 gttcagtttg gaaatggtaa catcatgtgt ttccactttt tgctgaactt gttcgagcgg
     3421 agttaaattt tcatcctgcg ctgtggcggt aaataccgat gttgcaatgg caacggtgta
     3481 aagaatactt tttaatttca tacttttatt tttcatttaa gtgaattgtc tttatataag
     3541 tatttgtttt ctgtcgaatt tagtacaatg taaacttcat tgatttaatt ttcacgacac
     3601 aaaattatac ctacattatt acaataatat tacacaaaca ttaaaaagaa aagaaagcag
     3661 aatgcagaat gcagaataaa gaatacaggt tttgaatagt attggtaggg aaagacgggt
     3721 gggtgataaa taaaaaaaaa tccctgcaac aggctgtagc agggatttta atatctgaat
     3781 ggaatgattt atttgtgcag tgagaagaca aactccagac ctccgcccgg tttgttatta
     3841 actgatatgg ttcctttatg agtttccact gcgtttttca caatcgaaag tcccaaaccg
     3901 gtaccgccgg ttttacgcga gcggcctttg tctacccggt agaaacgatc aaacagtgat
     3961 gataaatgtt cgggaccaac acccatacca ttgtcagaaa atgaaaaaaa gaaatatttt
     4021 gcatcttcac cggttagggc aatgaaaatt ttcactttat cgcctccgta ggctatggaa
     4081 ttatccatca gatttcggaa aatggaatat aaaagcattt gattgccgcg cagccataca
     4141 tcaccgccaa acgaggtatc gacggtaatg tttttttcgt gaaggataat atccagatca
     4201 tccgttacat tttccactat tttatttatg cacacaggtt ccatttcaat agaatgaggg
     4261 gcttcgttca tctggttcag cagcagaaca tcctgcagca gttgatttag cctcaccgcc
     4321 tgcgaatgac ttcgttttat aaagaaatcg cgacgctcgg gagtgatttc aggattgttg
     4381 taaagtgtct ccatgtaccc gatgatgctg ctcaacggcg ttttaagttc atgtgccacc
     4441 tgttgggtca gttt
//
LOCUS       DAOPZK010000152         4378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_25889_length_4378_cov_20.083044, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000152 DAOPZK010000000
VERSION     DAOPZK010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25889_length_4378_cov_20.083044"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKH68_08065"
     CDS             <1..462
                     /locus_tag="PKH68_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12931.1"
                     /translation="SQTFVAMIPGLMIFHLIFPNFFPEIIHPVISFSVFVFPLTLLLS
                     FASGLLDGLTRFKTLETPLLKSKIIYSLIIVVLAGLVAILFKPETYNFWIVLLSLGAL
                     VCAVKLGLLGKKLINVILPGTYQRRKKKVTGKTAAKVAPKPKVEESPTAAE"
     gene            802..>2291
                     /locus_tag="PKH68_08070"
     CDS             802..>2291
                     /locus_tag="PKH68_08070"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNX12932.1"
                     /translation="MEKLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEALGVDVIEA
                     GFPVSSPGDFNSVVEISKAVTWPTICALTRGVQKDIEVAAEALKFAKRKRIHTGIGTS
                     EFHIKYKFNSTQDEILERAIAATKYAKKYVEDVEFYCEDAGRTDNVYLARVVEAVIKA
                     GATVVNIPDTTGYCLPWEYGNKIKYLFENVKGIQNAIISTHCHNDLGMATANTIAGVM
                     NGARQVEVTINGIGERAGNTSLEEVAMILKCHKSMEIETQINTQKIYPISRMVSSLMN
                     MPVQPNKAIVGRNAFAHSSGIHQDGVLKNRESYEIMDPKDVGIDENSIVLTARSGRAA
                     LKHRLSVLGVEVENGKLDGIYEEFLKLADRKKDINDDDVLVLAGMERSEQKRIKIDYL
                     QVTSGVGVKPVASLGLIIAGEKFEAAASGNGPVDAAIKALKQIIRRNMTLQEFTIQAI
                     SRGSDDVGKVHMQVEHQGAIYYGFGANTDIIAASVEAYIDAINKFV"
     assembly_gap    2292..2391
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2392..2976
                     /locus_tag="PKH68_08075"
     CDS             <2392..2976
                     /locus_tag="PKH68_08075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HNX12933.1"
                     /translation="MTEKQKMLAGLLYDANNDPDLIQERLDCKDKIYNYNRLRPSDLL
                     YRKTILLEILGKTGGNFIIEQPFYCDYGYNISIGENFYSNFNLVILDEASVTFGDNVF
                     IAPNVGIYTASHPLDAAARNLGQETANPVMIGSNVWIGAGVQILPGVSIGDNSVIGAG
                     SIVNKSIPANVLAVGNPCRVVREIAPKIPKEEAP"
     gene            3108..>4378
                     /gene="leuC"
                     /locus_tag="PKH68_08080"
     CDS             3108..>4378
                     /gene="leuC"
                     /locus_tag="PKH68_08080"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNX12934.1"
                     /translation="MTNNITNVQIPPSGGGGATLFDKIWNAHVVSEVENGPTQLYIDR
                     HFCHEVTSPQAFNGLRTRGLKVFRPEKTTMTADHNTPTINQDQPVKDPVSRHQLDTFA
                     RNAAEFGMPIFYPLGHEKNGVVHIIGPENGFTQPGMTIVCGDSHTSTHGAFGTIAFGI
                     GTSEVEMVLATQCILQTRPKTMRITFNGKLGKNVLAKDLALFMISKLTTGGATGYFLE
                     YAGEAIRNLSMEQRMTICNLSIEMGARGGLIAPDETTFNYVKGREFAPKGEEWDKKLA
                     YWKTLKSDDDAVFDKELTFDASEIEPMVTYGTNPGMGMGISGTIPTLDEIDEAGRISY
                     LKSLDYMGFKPGEKMLGKPVDYVFLGSCTNGRIEDFRIFANYVKGKKKADNITAWLVP
                     GSWKVEKQIREEGLYDILTEAGFQLRQPGCSA"
BASE COUNT         1291 a          946 c          988 g         1053 t
ORIGIN      
        1 tcgcagactt ttgtggccat gataccaggt ctgatgatat ttcaccttat tttccccaat
       61 tttttcccgg aaataatcca tccggttatc agcttctctg tgttcgtttt cccgctgact
      121 ttgttacttt cctttgcttc gggtctcctc gacggactga cccgcttcaa aacactggaa
      181 acacctctgt tgaaatcaaa aattatttat agtttaataa ttgttgtact tgccggtttg
      241 gtggccattt tgttcaagcc cgaaacctat aatttctgga ttgtactgct gagtctggga
      301 gctttggttt gtgctgtaaa actgggactt ttgggtaaaa aactgatcaa tgtgatttta
      361 cccggaacct atcagcggag aaagaaaaaa gtaaccggca aaactgctgc aaaagttgct
      421 cccaagccta aagtggaaga aagtccgact gctgctgaat aaaatcataa tataatcagt
      481 aataaataaa acagggcaat cgaaatattg cccttttttg tatcattatc aaacaaaaag
      541 attggaaatt ctaaaaaatt ataagaataa atatcaatta ttgttcaatt cgtattgcgg
      601 aattgaaatt tagttatatc tttgcaacgt tttaatcaaa agaacaatga caactaatta
      661 catccatatt ctcctactcg gtattattat tctcttgcaa aacgagacga agtgagtttg
      721 gtatgtatat aatgaatgaa taagaaatat ataaaagcct ttctcacata agagaagggc
      781 ttttttttaa taaaaaaatc aatggaaaaa ctatttattt ttgatacaac attgcgcgac
      841 ggagaacagg ttcccggttg ccagttgaac acagtggaaa aaattcaggt tgccaaagcg
      901 cttgaagcat tgggagtgga cgttattgaa gctggtttcc ccgtttcaag tccgggtgat
      961 ttcaattcgg tagtggaaat atccaaagcc gtgacatggc caaccatctg tgccctcaca
     1021 cgtggtgtgc agaaagacat cgaagtggct gccgaagccc tcaaatttgc aaagcgtaaa
     1081 cgaattcata ccggtattgg aacttcggaa tttcatataa aatataaatt caattccaca
     1141 caggatgaaa ttctggaacg ggccattgct gccaccaaat acgccaaaaa gtacgtggaa
     1201 gatgtggaat tttactgcga agacgccggc cgcaccgaca acgtgtacct ggcccgcgta
     1261 gtggaagccg tgattaaagc cggtgccacg gtggtcaata tccccgacac aacgggctac
     1321 tgcctgccat gggaatatgg caataaaatt aaatacctgt ttgagaatgt aaagggtatt
     1381 caaaatgcaa tcatctccac ccattgccac aacgacctgg gcatggctac tgccaatacc
     1441 attgccggag tgatgaacgg agcccgccag gtggaagtaa ccatcaacgg cattggcgaa
     1501 cgcgccggaa acacctcgct cgaagaagtg gccatgatac tgaaatgcca caaaagtatg
     1561 gagattgaaa ctcaaatcaa tacgcagaaa atttatccga taagccgcat ggtttccagc
     1621 cttatgaata tgccggttca gcccaacaaa gccattgtgg gacgaaatgc gtttgcccac
     1681 tcatcgggta ttcaccagga cggcgtactc aaaaaccgag aaagctacga aataatggac
     1741 ccgaaagatg tgggcatcga cgaaaactcc atcgtactta cagcccgaag cggacgcgcc
     1801 gccctcaagc accgcctcag tgtgctgggt gtggaagttg aaaacggcaa actggacggc
     1861 atttacgaag agtttctgaa acttgccgac cgaaagaaag atatcaacga cgacgacgtg
     1921 ctggtattag ccggtatgga acgctctgaa caaaaacgga taaaaatcga ttatttacag
     1981 gtgacctcgg gagtaggagt taagccggtg gccagcctcg gactgatcat tgccggcgaa
     2041 aaatttgaag ctgccgccag cggtaacggc ccggtagatg cagccatcaa ggctttgaaa
     2101 caaatcatcc ggcgcaacat gaccctgcag gaatttacca tccaggccat cagccgcggc
     2161 agcgacgatg tgggaaaagt gcatatgcag gtagagcatc agggtgccat ctattacgga
     2221 tttggcgcta ataccgacat cattgccgct tcggtggagg cgtatattga tgctataaat
     2281 aaatttgtga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgacagaa
     2401 aaacaaaaaa tgctggcagg gctgttgtac gatgccaata acgatccgga tttgatacag
     2461 gaaaggttgg attgcaaaga taaaatatac aactacaacc gcctgcgacc ttcagacctt
     2521 ctgtaccgga aaacaatttt actcgaaatc ctaggcaaaa cgggcgggaa cttcatcatt
     2581 gagcagccgt tttactgcga ttatgggtac aatatatcga ttggtgaaaa tttttattcc
     2641 aattttaatc tggtaatact ggatgaggca tccgttactt ttggcgataa tgttttcatt
     2701 gctcccaatg tgggaattta caccgcttca catccgctcg atgcagccgc acgaaacctc
     2761 ggacaggaaa cagctaatcc ggtgatgatt ggcagcaatg tttggattgg agccggtgtg
     2821 cagattttgc cgggggtaag tattggcgac aattcggtaa tcggggcagg aagtattgta
     2881 aacaaatcaa ttccggcaaa cgttttagct gttggtaatc cctgcagggt ggtgagggag
     2941 attgccccta aaatccctaa ggaagaagcc ccctaaatcc cccaaagggg gactttaaaa
     3001 cactttagta tttaaaaaac tgtaaattct gtaagattaa agatagaacc atatttactt
     3061 aattcgtgta attcgtatta attcgtgtaa ttcgtaatat taaaaatatg acaaataaca
     3121 taacaaacgt acaaatcccc ccttcggggg gtgggggggc taccctcttc gataaaattt
     3181 ggaatgctca cgtggtttcc gaagttgaaa acggtcctac ccaactctac atcgaccgcc
     3241 atttctgcca cgaagtaacc agtccgcagg catttaacgg attgcgtacg cgcggcctga
     3301 aagttttccg tccggaaaaa accacgatga ctgccgatca caacacgccg accatcaatc
     3361 aggatcaacc ggtaaaggac ccggtttcgc gccatcagct cgatactttt gcccgtaatg
     3421 cagccgaatt cggtatgccc attttctatc cgctcggaca cgaaaagaac ggcgtagttc
     3481 acatcatcgg acctgaaaac gggtttacac aacccggtat gaccatagtt tgcggcgaca
     3541 gtcacacatc tacgcacggt gctttcggaa ccatagcttt cggtattggc accagcgaag
     3601 tggaaatggt gctggctacg cagtgcatcc tgcaaacccg cccgaaaacc atgcgcatca
     3661 cattcaatgg taaactggga aaaaatgtac tggccaaaga cctggcgctt tttatgattt
     3721 cgaaactcac aacaggtggt gctacgggct atttcttgga atatgccggt gaagccatcc
     3781 gcaacctgag catggaacaa cgcatgacca tttgtaacct ttccatcgaa atgggtgcca
     3841 ggggcggact gattgcaccc gacgaaacca cctttaacta cgtgaaagga cgtgaatttg
     3901 ctcccaaagg cgaagaatgg gacaagaaac tggcttactg gaaaacatta aaatcggatg
     3961 atgatgccgt ttttgacaaa gagctgactt ttgatgcttc cgaaatagag ccgatggtca
     4021 cttacggcac caatcccgga atgggaatgg gcatcagcgg cacaatacca actttggatg
     4081 aaattgatga agccggacgc atatcatatc tgaaatcact tgactatatg ggtttcaaac
     4141 cgggtgaaaa aatgctcgga aaaccggtgg attatgtttt tctgggcagt tgtaccaatg
     4201 gccggatcga agatttccgc atttttgcca actacgtaaa agggaagaaa aaagcggata
     4261 atatcacagc ctggctcgta ccgggaagct ggaaggtgga aaaacaaatc cgcgaagagg
     4321 gcttgtacga catactcacc gaagcaggtt ttcagcttcg tcagcccggt tgctcggc
//
LOCUS       DAOPZK010000153         4359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_26051_length_4359_cov_24.491171, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000153 DAOPZK010000000
VERSION     DAOPZK010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26051_length_4359_cov_24.491171"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKH68_08085"
     CDS             complement(<1..263)
                     /locus_tag="PKH68_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HNX12935.1"
                     /translation="MNYTKLILLQLIIFLSIIATAQNKVTSKIMELGKTDNRTMQHLD
                     VLSNRLGPRPIGSDAYENATYWVADQLRKWGLEVEIQKVGELPV"
     gene            777..1532
                     /locus_tag="PKH68_08090"
     CDS             777..1532
                     /locus_tag="PKH68_08090"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNX12936.1"
                     /translation="MMDKIRKKYYSLVKKFHQLENDISDNAIHDKRVILCKVRAVLLV
                     LDTKEKHIHNGNKVFALMGKMRNLQVQRLILLSDERKSETYPYILYLEIKLKKNGMKI
                     RKFIRKHQVRFPEIDRTKKTDAEQIKSELLLAHDKLRKMIVAIDTYEAATIHKIRKSF
                     KRFRYLIEILSIVKTVDKETLDKLKEFQDRLGEIQDFRMLIKGLNQFSLKINSPIDIL
                     PFESIQTKLICKFSEEIERFIAFCEQTTLIAVE"
     gene            complement(1613..3916)
                     /locus_tag="PKH68_08095"
     CDS             complement(1613..3916)
                     /locus_tag="PKH68_08095"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNX12937.1"
                     /translation="MAKLTREDALLYHSQGKPGKIEVVPTKPYSSQRDLSLAYSPGVA
                     EPCLEIEKESAKAYEYTSKGNLVAVISNGTAVLGLGDIGPLAGKPVMEGKGLLFKIFA
                     GIDVFDIEVNEKDPEKFIQVVKAIAPTFGGINLEDIKAPQCFEIEERLKEELDIPVMH
                     DDQHGTAIISAAGLINALDIIGKKIEDIKIVVNGAGAAANSCTKLYMALGAKLENIVM
                     VDSKGVINTKRTDLNDRKKPFATTRDITTLAEAVEGSDVFLGLSVADVLTTEMVQSMN
                     ENPIVFALANPNPEIAYDVAIAARPDIVFATGRSDHPNQINNVLGFPYIFRGALDVRA
                     NTINEEMKVAAVKAIAELAKKPVPDVVNSAYNMKKISFGRDYIIPKALDPRLLTTVAP
                     AVAKAAIESGVAAKIIEDWEAYDHQLQKLMGLDNKMLRHFYEAARQNPKRVVFSESLH
                     INMLKAAETALAEGICQPILLGNEEKLANVAAENGIDLTGIQIVNLRHDREEERRTRY
                     AKLLSEKRSREGMTFEEAFEKMYERNYFGMMMVETGEADALITGVITKYEDSVKAART
                     VIGARPGVNSIGAMHIMNTKKGTYFLADTLFNRHPSTETIIDVAKLTYDTVKFFAHEP
                     VMALLSYSNFGSDKEGSPASVHKVNQVLQERYPDMVVDGEMQANFALNKELRDRKYPF
                     SRLKGKDVNTLIFPNLSSANMVQQMMLELGLAETIGPIQMGLNKPVHILDVESSVRSI
                     VNMTAVAVLDAIVQEKIEKGEVERIGE"
     gene            complement(4023..>4359)
                     /locus_tag="PKH68_08100"
     CDS             complement(4023..>4359)
                     /locus_tag="PKH68_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNX12938.1"
                     /translation="QAIPIVHFDWDDNKSMAANNSSSFCYRNVANTIRKSRHSTGMAI
                     DINPYFNPIRYKSPQQSKPNTPEGSVLDTTVAGTLYAVHPVVKEFKRRGFRWGHHFRK
                     YFDDHHFDK"
BASE COUNT         1249 a          890 c          846 g         1374 t
ORIGIN      
        1 accggcaact ctcccacttt ctgaatttcc acctccagtc cccactttcg tagctggtcg
       61 gctacccaat aagtggcatt ttcgtacgca tcagacccta tcggacgggg tcctaagcgg
      121 ttactaagca catccaggtg ttgcatggtg cggttgtcgg ttttaccaag ttccattatt
      181 ttactggtaa ctttgttttg agcagttgca attatggata aaaatatgat taactgcaac
      241 agtatgagtt tcgtgtaatt catgtttgtg taaatttttt ggtgcatatg cccagcaaag
      301 atataaaaac aagagaatat aaaattattt aagtagtaaa acctgaagtt tatcttattt
      361 tggagcttta tcgcaaccat tgtctcttta aaataatcaa tcagaatgct gagatcagga
      421 cattctgatt gagttattaa aaaaaatgaa tgtaaacaaa cagatatagg tataattagg
      481 cttatagagt gatatactta aacaatgttc cggatttact gaagtctgat taatattatt
      541 acgcaaagaa acaacggatt tttgacacct tttgtcactt ctaaattatt tacctttata
      601 ttgtgttaaa ttattcggta ttgattattg aagatgtttt ttttaaacaa taaatagaac
      661 gaaaatgttt aataattatc aaattaccaa atattttcgg atagagtaaa ccattcgatg
      721 tgttctaaaa atttggttga attgtgtttt ttccttcaat tatagtaacc aaataaatga
      781 tggacaaaat cagaaaaaaa tattattcac tggtaaagaa atttcatcaa ctcgaaaatg
      841 acatttctga taacgccatt cacgacaaaa gggttatatt atgcaaggtt cgtgcagtac
      901 tcttggtact ggatacgaag gaaaaacaca tccataacgg gaacaaagta tttgcgctga
      961 tgggtaaaat gcgaaatctg caggttcaga ggttaattct gctttccgat gaaagaaaat
     1021 ctgaaacata tccctatatt ttatatcttg aaataaaatt aaaaaaaaac ggtatgaaaa
     1081 tcaggaagtt tatcagaaaa catcaggtca ggtttcccga aattgaccga acaaaaaaaa
     1141 ccgatgcaga acaaataaaa tcagagctgc ttctggcaca cgataaactc aggaagatga
     1201 ttgttgcaat tgatacttat gaagcagcta ctatacataa aatcaggaag tcattcaaga
     1261 gatttcgcta tttgattgaa attctttcga ttgtaaaaac tgttgataaa gagacacttg
     1321 ataaattaaa ggaatttcag gacaggctgg gtgaaataca ggatttcagg atgttgataa
     1381 agggcttaaa tcaattcagc ctgaaaatca actctccgat tgatatttta ccgtttgaga
     1441 gtattcaaac gaagttaatc tgtaagtttt cggaggagat tgaaagattt atagcttttt
     1501 gtgagcaaac tacccttata gcagtggaat aaacaaaaaa acagagttaa tccgaagact
     1561 aactctgcct gaatttcaaa aattatgaga tgaaactata agattttttt ccttactcac
     1621 caattcgttc tacctcgccc ttctctattt tttcctgaac aattgcatcg agtacagcca
     1681 ctgcagtcat gttcacaata ctgcgaaccg aactttccac atccagaata tgtacgggtt
     1741 tattcaatcc catctggatt ggtccgattg tttcggccag acccagctca agcatcatct
     1801 gttgcaccat gttggctgaa ctcaggttgg gaaaaatcag tgtgttaaca tctttgcctt
     1861 tcaggcgcga gaatggatat ttacgatcgc gaagttcctt attgagtgca aaatttgcct
     1921 gcatttcgcc atcgaccacc atatcgggat aacgctcctg caatacctga ttcactttgt
     1981 gtacacttgc agggctgcct tctttgtccg atccgaaatt ggaataagac agcaaagcca
     2041 tcacgggttc gtgagcaaag aattttactg tatcataagt aagttttgct acgtcgatga
     2101 tcgtttcggt tgaaggatgg cgattaaata aagtatcagc cagaaagtaa gtgcctttct
     2161 tagtgttcat aatgtgcatt gctccaatgg agttaactcc cggacgggca ccaatcacgg
     2221 ttcgtgcagc ctttactgaa tcttcgtatt tcgtaatcac accggtaatc agggcatctg
     2281 cctcgcctgt ttccaccatc atcatgccga aatagttgcg ttcgtacatt ttttcaaatg
     2341 cttcttcgaa tgtcattccc tcgcggctgc gtttttctga gagcagtttt gcatagcgtg
     2401 tgcggcgttc ttcttcccgg tcatgacgga gattaacaat ctgaattccg gtaaggtcta
     2461 tgccgttttc ggcagccaca tttgccagct tttcttcgtt tccaagcaaa attggttgac
     2521 aaattccttc tgccaatgcc gtttcggcag ctttaagcat attgatatgc agcgattccg
     2581 aaaatacaac tcgttttgga ttttggcgcg cggcttcgta aaaatggcgc agcattttat
     2641 tgtcaagtcc cataagcttc tgaagctgat gatcataagc ttcccagtct tcaattattt
     2701 tggccgccac tcctgattcg atagcggctt tggccacagc aggtgcaact gtggttagta
     2761 atcttgggtc aagtgctttg ggaataatat aatcgcgtcc gaagctgatt tttttcatgt
     2821 tgtaagccga gtttaccaca tcaggaaccg gctttttagc cagttcggca atggctttca
     2881 cagctgccac tttcatctcc tcgttgatgg tattggctcg tacatccagt gcaccacgga
     2941 aaatgtacgg gaaaccgagc acattattga tctggttcgg atggtccgat cgaccggtgg
     3001 caaatacaat atcaggtcgc gcagcgatgg ctacatcata agcgatttcg ggatttggat
     3061 ttgccagtgc aaacacaatc gggttttcgt tcatgctctg caccatttca gtggtcagca
     3121 catcggcaac tgataaaccc agaaaaacat ccgaaccttc aacagcctca gccagtgtag
     3181 tgatatcacg agtggttgca aagggttttt tacggtcgtt cagatcggta cgttttgtat
     3241 tgataacccc tttcgagtca accattacaa tattttccag tttcgcaccc agcgccatgt
     3301 ataattttgt acatgaattt gcggctgctc cggctccgtt taccacaatt tttatatcct
     3361 ctattttttt cccgataata tccagtgcat taatcagtcc agccgcagaa atgatggctg
     3421 taccatgctg atcatcgtgc attacgggta tatccagctc ctctttcagt ctttcttcta
     3481 tttcaaagca ctgcggtgct tttatatctt ccagattaat accaccgaag gttggggcaa
     3541 tggctttcac cacctgaata aatttctcgg gatctttttc gttcacctca atatcaaata
     3601 catcaatccc tgcaaaaatt ttgaacaaca acccctttcc ttccattacc ggttttcctg
     3661 caaggggtcc tatgtcaccc aaacccagca cagccgtacc atttgaaatc acagcgacca
     3721 agtttccctt tgatgtatat tcatacgctt ttgcactttc cttttctatc tcaaggcacg
     3781 gttcggccac tcccggtgaa tatgcaagtg ataaatcccg ttgtgaactg taaggttttg
     3841 tgggaacgac ctcaattttt cccggtttgc cctgtgaatg ataaagcaag gcatcctctc
     3901 ttgttaattt tgccatttta ttttttaatt cgtaaattat tgtgtgtttt ttctttgaaa
     3961 tattttgcaa aggtagttac attatttcat tccgtaattc aacaatcgct tcattttaac
     4021 tattatttat caaaatgatg atcgtcgaag tatttgcgga agtgatgtcc ccatctgaag
     4081 ccccggcgct tgaattcttt cacaacagga tgtaccgcat agagagttcc tgcaactgta
     4141 gtatccaaaa cggaaccttc gggagtgttt ggcttgcttt gttggggtga cttataacga
     4201 atgggattga aatagggatt aatatcaata gccatgccgg tggaatgcct tgatttcctt
     4261 attgtgttgg ctacattcct gtaacaaaag cttgaagaat tattggcagc catggattta
     4321 ttgtcatccc agtcaaaatg aactatcgga atagcctga
//
LOCUS       DAOPZK010000154         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_26095_length_4354_cov_27.832519, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000154 DAOPZK010000000
VERSION     DAOPZK010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26095_length_4354_cov_27.832519"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKH68_08105"
     CDS             <1..846
                     /locus_tag="PKH68_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNX12939.1"
                     /translation="ATLQEKRSLVCAQCHVEYFFKGEGKYLTFPWDKGTTVEDMEKYY
                     DETDYADYTHALSRAPILKAQHPDYEIAQFGIHGQRGVACADCHMPYKSEGGIKFSDH
                     HIQSPLAMMDRTCLVCHRESEETLRQNVYDRQRKVNETRTRLEEELAKAHIEAKFAWE
                     KGATEVQMKPVLNFIRQAQWRWDYGVASHGAAFHAPQEIQRVLSHGIDRAMQARLANA
                     KVLAKLGFSGDVPLPDISTKEKAQKYIGLDIPTERKNKDKFMKEIVPEWLKKAKAENR
                     IIAAK"
     gene            865..>1154
                     /locus_tag="PKH68_08110"
     CDS             865..>1154
                     /locus_tag="PKH68_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12940.1"
                     /translation="MWKYPWGYKEGFAINAGLFTTGILLQLSIGRFSTDFLSFPVNFI
                     SGLLFIFLLTGLHILSRKNRQIRWFSGMEATITSVSSFLILVIVMGLIRQHSG"
     assembly_gap    1155..1254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1337..2128
                     /gene="ccsA"
                     /locus_tag="PKH68_08115"
     CDS             1337..2128
                     /gene="ccsA"
                     /locus_tag="PKH68_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNX12941.1"
                     /translation="MSWTTFIYFALPAVILWGAGAYLSYREKKSFAVGISFFGLLVFG
                     TFIAGMWISLERPPLRTMGETRLWYSFFLPVAGLITYLRWNYKWILTFSTVLSFVFIC
                     VNLLKPEIHNKTLMPALQSPWFAPHVIVYMFSYAMLGAAAVIALYLLIKKGDKKPEDN
                     VMDLLDNLVYVGVAFLTLGMMMGALWAKEAWGTYWSWDPKETWAAATWLSFLVYIHYR
                     NHRPKHIKRSLAVLVFSFVLLQVCWYGINYLPSAKGVSVHTYNVE"
     gene            2160..>3160
                     /locus_tag="PKH68_08120"
     CDS             2160..>3160
                     /locus_tag="PKH68_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNX12942.1"
                     /translation="MKTKLLIISLLIGSSLIFSQEQQAPKENEFSLTFQLRPRAEYRN
                     GVLFPRNENDKAAAFINNRTRLSLDYARKGLSMGLSLQNTGVWGDMPQINKTANVSIY
                     EAWAKLSTTGGLFAKFGRQSLVYDDERLLGALDWNVNGRSHDAIKLGFENKEHKLHAV
                     LAFNQNEEKRIGGTYYIAAQPYKTMQTLWYQFNGNSTFKPSVMIMNLGYETGNFSAGV
                     SEVTNMQTIGTFMLLKPSKELGLNFSGYYQTGNNAGKSQVSAYLLALKGMYDISPKVA
                     LTGGVDYLSGEGNTDNHAVINAFNPLFGTHHKFYGAMDYFFASSFANNLNPGLADIYV
                     "
     assembly_gap    3161..3260
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3311..3871)
                     /locus_tag="PKH68_08125"
     CDS             complement(3311..3871)
                     /locus_tag="PKH68_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNX12943.1"
                     /translation="MKPVYFNNQIEFRQWLAENHADKNELLVGFYKISTGKPSMTWSE
                     SVDQAICYGWIDGIRKTVDAESYCIRFTPRKPTSTWSKVNIQKAECLIKNGLMTASGL
                     EAYNKRSMENSGIYSFENEEKVLPFEFERKFKTNHTAWDFFSNQAPPSYRKTVIHWIL
                     SAKQEITRISRLEKLIAASEQQKRIY"
     gene            complement(3876..>4354)
                     /locus_tag="PKH68_08130"
     CDS             complement(3876..>4354)
                     /locus_tag="PKH68_08130"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNX12944.1"
                     /translation="NAQPEIKTTNYSRIGTIEKFEFTAGDEQGAEITFTTALRQYLYE
                     PNAAIMKSGAFGLISEKFRLNKLHINSHLYTSDRLIDDFPGRIFCVQTTIDFSKKSLS
                     NLNDEVKKANLTIRNFPLTVNELRKKLKIPEGGDVYLFATTLADNKKVLVKCSKVK"
BASE COUNT         1177 a          848 c          907 g         1222 t
ORIGIN      
        1 gccaccttgc aggaaaagcg ttcgctggta tgtgcgcagt gccatgtgga atattttttc
       61 aagggagaag ggaaatacct cacattcccc tgggacaaag gaactacagt ggaagatatg
      121 gagaaatatt acgacgaaac ggattatgcc gattacaccc atgccctgag ccgtgcaccc
      181 attctcaaag cacagcatcc cgattatgaa atcgcccagt ttggcattca cggtcagcgc
      241 ggagtggcct gtgccgactg tcacatgcct tacaaaagcg aaggaggtat aaaattcagc
      301 gatcatcaca ttcaaagtcc gctggcaatg atggatcgca cctgtctggt atgtcatcgg
      361 gagtcagaag aaacactccg gcagaatgtg tacgatcgtc aacgcaaagt gaatgaaacc
      421 cgtacccgac tggaagaaga actcgccaaa gcacacattg aagcaaaatt tgcctgggaa
      481 aaaggggcaa ctgaagtgca gatgaaaccc gttttgaatt ttatccgtca ggcacaatgg
      541 cgctgggatt atggtgtggc atcgcatgga gcagcatttc acgcacctca ggaaattcaa
      601 cgtgtactga gtcacggcat cgacagagcc atgcaggcac gactggccaa cgccaaagtg
      661 ctggcaaaac taggatttag cggtgatgtg cctttgcctg atatttctac caaagagaaa
      721 gcacaaaaat atattggcct ggatattccc accgaaagaa agaataagga caaattcatg
      781 aaagaaatag ttcccgaatg gcttaaaaaa gcaaaagcag aaaaccgaat tattgcagcc
      841 aaatagaata aacaacgaaa tagtatgtgg aaatacccct ggggttataa agaaggattt
      901 gctataaatg ccggtttatt cacaaccggc attctcttac aactcagtat cggtaggttt
      961 agtaccgatt ttctatcgtt tccggtaaat tttatatcag gtttattatt tatttttctg
     1021 ctgaccggtt tgcacatcct ttcacgcaaa aacagacaaa ttcgttggtt ctcggggatg
     1081 gaagccacca tcacttcggt atcgtcgttt ctgattttag taattgtcat ggggcttatt
     1141 cgccagcact ccggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagaagt
     1261 cagaagtcag aagttagaaa ttagaaatta gaagttagaa atcacagata tttaattctt
     1321 tatcaaatat ttgagtatgt cctggaccac atttatatat tttgctttac ctgcagttat
     1381 tctttggggt gcaggtgctt atctttcata cagagaaaaa aaatctttcg ctgtagggat
     1441 tagctttttc ggattgcttg tgtttggcac cttcatcgcc ggcatgtgga tttcgctgga
     1501 gcgtccgccg ttgcgcacaa tgggcgaaac ccgattgtgg tattcgtttt ttctgcccgt
     1561 tgccggactg ataacttacc tgcgctggaa ttataagtgg atacttactt tcagcacggt
     1621 tctgtccttc gtatttattt gtgtcaatct cctgaaaccc gaaattcaca ataaaacact
     1681 gatgcccgca ctgcaaagtc cgtggtttgc acctcatgtg atagtttata tgttttcgta
     1741 tgccatgctc ggagctgccg cagttattgc actttacctt ttaataaaaa aaggagataa
     1801 aaaacccgag gataatgtaa tggatttgct ggacaatctt gtctatgtgg gagttgcttt
     1861 tcttacgctc ggtatgatga tgggagcact ttgggcaaaa gaagcctggg gtacctactg
     1921 gagctgggac cccaaagaaa cctgggcagc tgctacctgg ctgagttttc tggtgtatat
     1981 tcattaccgc aatcaccgtc ccaaacacat caaacgttca ctggcagtgc tggttttctc
     2041 atttgtcttg ttgcaggtat gctggtatgg catcaactac ctgccatcgg ctaaaggggt
     2101 aagtgtacac acctataatg tggaatagaa aaaaatatat aatctaaata agtaagatta
     2161 tgaaaacaaa attattgatt attagtttgt taattggaag ttcattaatc ttttctcagg
     2221 aacagcaagc cccaaaagag aatgaatttt ccctgacatt tcagttgcga ccaagagcag
     2281 aatatcgtaa tggcgtttta tttccccgaa atgaaaatga taaggcagct gcatttatca
     2341 ataaccgaac ccgtttatcg ctggattatg cccgcaaagg gttgagcatg gggctttcgt
     2401 tgcaaaatac gggagtttgg ggtgatatgc cgcaaataaa caaaacggct aatgtcagta
     2461 tttacgaagc atgggcaaaa ctatcaacta ccggcggact ttttgccaag tttggccgtc
     2521 agtcgttggt ttatgacgat gaacgcctgc tgggggcact cgactggaat gtaaatggac
     2581 gcagtcacga tgccattaaa cttggtttcg agaacaaaga acacaaactg cacgctgtac
     2641 tggcattcaa ccaaaatgaa gaaaaacgaa taggaggcac ctattatatt gctgctcagc
     2701 cttataaaac catgcagacg ctgtggtatc agttcaatgg taattcgaca ttcaaacctt
     2761 cggttatgat tatgaacctg ggttacgaaa caggaaattt ttcagccgga gtatccgaag
     2821 tgaccaatat gcaaacaatc gggacgttta tgctcctgaa accgtcaaag gaacttggat
     2881 taaatttttc gggttactat cagaccggaa ataatgccgg gaaaagtcag gtatctgctt
     2941 accttcttgc tctgaaggga atgtatgata tttcgccaaa agtagcgctc acgggtggag
     3001 tcgattatct gagcggtgaa ggcaataccg acaatcatgc cgtcatcaat gctttcaatc
     3061 ccttatttgg tacacatcat aagttttacg gtgctatgga ttattttttt gcaagttcat
     3121 ttgccaataa tcttaatcca ggattggctg atatttatgt nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn attgtcaatg tgaaaggaga aaatgaatgt gttttctcct
     3301 tttattgttc tcaataaatc cgtttttgtt gttcgctggc tgcaattagt ttttcaagcc
     3361 gggagatgcg tgtaatttcc tgcttggccg acaagatcca gtggataact gtttttcgat
     3421 acgatggtgg agcttgattg ctgaaaaaat cccacgccgt atgatttgtc ttgaattttc
     3481 tttcaaattc aaatggaagt actttctctt cattttcaaa actatatatt cccgaattct
     3541 ccatgcttcg tttattgtat gcttccagcc cggaagcagt catcagtccg tttttgatca
     3601 ggcattcagc tttttgaatg ttgaccttgc tccaggtact tgtgggctta cggggagtga
     3661 aacgtatgca gtagctttcg gcatctactg ttttgcgaat tccatcgatc caaccgtaac
     3721 agatagcctg atcgaccgac tcgctccagg tcatggacgg ttttccggtg ctgattttgt
     3781 aaaacccaac caacaattca tttttatcgg catgattttc agcgagccat tggcggaatt
     3841 caatctgatt gttaaaatag acaggtttca tagacttatt tcactttgga gcatttcacc
     3901 aatacttttt tattatcagc cagggtagtg gcaaaaagat acacatctcc tccttccgga
     3961 attttcaatt tttttcgcaa ttcattcact gtcagcggga aattccgaat agtcagattc
     4021 gcttttttca cttcatcgtt cagattactg agagatttct tgctgaaatc aatagtagtt
     4081 tgcacgcaaa atatacgtcc cggaaaatca tcaattagac gatcagaagt atataaatga
     4141 ctgttgatgt gcaatttatt caacctgaat ttctcactta tcagtccgaa ggcaccgctt
     4201 ttcataatgg cagcattcgg ttcatacaaa tattgccgga gagcagtggt gaatgttatt
     4261 tcagcaccct gttcatcacc ggctgtaaat tcaaattttt cgattgtccc aattctggaa
     4321 taattggtag tttttatttc aggttgcgca ttgt
//
LOCUS       DAOPZK010000155         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_26214_length_4341_cov_23.394074, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000155 DAOPZK010000000
VERSION     DAOPZK010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26214_length_4341_cov_23.394074"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKH68_08135"
     CDS             complement(<1..630)
                     /locus_tag="PKH68_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNX12945.1"
                     /translation="MKKALIIGSTGMIGAELTRQLLEDNNYTEVVSFVRRASGVKHPK
                     LTEQIVDFDKPESWKELLKGDVLFSAMGTTIASAKTKDNQYKVDFTYQYQTAQMASDN
                     GVPAYVLVSAAGASPRALAFYMNMKGELDEAVKRLPFKYIHIFRPGQLDGDRKENRTG
                     ERIALKLVYFFNKLGIARKYRPIHATQLAKAMIIAAGYTASGVYTLDEIF"
     gene            complement(640..1863)
                     /gene="serB"
                     /locus_tag="PKH68_08140"
     CDS             complement(640..1863)
                     /gene="serB"
                     /locus_tag="PKH68_08140"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNX12946.1"
                     /translation="MNSEIILITISGEDKPGVTNAITNILGNNNATILDIGQADIHHS
                     LSLGILFKTPDDSNSGNILKEVLFKCNELNMHVRFKPVSEENYENWVNRQGKSRYIVT
                     ILGKEIGARQLAMVTRELALQELNIDSIKRLTGRPSLHEQEENLRSCIELSVRGELKD
                     KNLLSEQFMLLTAREGIDISFQKDNIFRRNRRLICFDMDSTLIKTEVIDELADRAGVG
                     EQVRAITESAMRGEIDFTESFAQRVALLRGLDVSVMEEIARSLPIMEGADRLMSVLKM
                     CGYKIAILSGGFTYFGNYLKQRFDVDYVYANELEIADGKLTGRYLGDVVDGKRKAELL
                     KLIAQVEKIQLEQVIAVGDGANDLPMLNLAGLGIAFHAKPKVKESASQSISTIGLDGI
                     LYFLGFRDIHIDQEI"
     gene            complement(1903..3231)
                     /locus_tag="PKH68_08145"
     CDS             complement(1903..3231)
                     /locus_tag="PKH68_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNX12947.1"
                     /translation="MSCKSTNEPEKYAVTDGKALQKNIFPKDTLHVVTQYGATSYFIY
                     RDEVLGYDYELAQNLAAHLHLTLKVEVAKNDREMTDYLRTGKADLAIYTTYETKALKQ
                     EFNFVFAHDDSYLVLVQMMGRDAVSDPIELTGKEVWVKKHSIDHERLEALNEEIGGGI
                     IIKTNDTLNTDDLMLQVAANKIKYTIAYRNKALLQKIFNRTLDCRIPIGFSQRNGWLV
                     RKDDSALRDSIGAWSKLDATNYLKEKLYGKYWEKNPYFAFKKIPIPKGSISPYDNIFK
                     KHAKDIGWDWQLLAALAFVESGYDSTVVSWAGAKGIMQLMPQTALDFGVDQYEIENPE
                     KNIAAGVEYIKSLNMIYRKIADKEERIKFILASYNCGPAHILDAMALAEKYGKNPHVW
                     YDSVEYYLAKKSDPEFYNDPVVKYGFFRAKEPIRYVPNVLDTYNKYMGIR"
     gene            complement(3287..4012)
                     /locus_tag="PKH68_08150"
     CDS             complement(3287..4012)
                     /locus_tag="PKH68_08150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX12948.1"
                     /translation="MNPLLQKIMFIDIETVPMTSCYSEMNEELQHLWDEKFRSLQKRM
                     PEKYNDYTTPAQAFENSAGIYAEFGRIICISVGFLFTKNNERFFRTKSFADDDEIRVL
                     ADFSQLLEKYPDYNFCGHNIKEFDIPYICRRMLINNLPMPLQLQINNRKPWEVNLMDT
                     LDYWKFGDYKNYTSLRLLTAVFGIPSPKDDIDGSQVARVYYKENNLPRIAAYCQKDVL
                     ATAQVWLRMNGHEIISSENVQHI"
     gene            complement(4093..>4341)
                     /locus_tag="PKH68_08155"
     CDS             complement(4093..>4341)
                     /locus_tag="PKH68_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNX12949.1"
                     /translation="LKVFVDADPDDRLIRVINRDIVERGRSVNKVMERYEQTVKPMHQ
                     QFIEPTKRYADLIIPQGGNNHIAIDILTKYIENNLNKK"
BASE COUNT         1027 a          982 c          974 g         1358 t
ORIGIN      
        1 aaatatctca tccagcgtat acacacccga tgcagtataa ccggcagcaa tgatcattgc
       61 cttagccagt tgagtggcat gaatgggacg gtattttcgg gctattccca gtttgttgaa
      121 gaaataaact aattttaagg caattcgttc accagtacgg ttttctttcc ggtcgccatc
      181 gagttgtccg ggccggaaaa tatggatgta cttaaaaggc agccttttta cggcctcatc
      241 cagttcacct ttcatattca tgtagaatgc cagagcacgg ggacttgcac ctgccgccga
      301 aacgagtaca tacgcaggta ctccgttatc cgaagccatc tgagctgttt gatactggta
      361 ggtgaaatcc accttgtact ggttgtcctt ggttttagcg ctggcaatgg ttgtacccat
      421 ggcagagaag aggacatcac cttttagcag ttctttccag ctttcgggtt tgtcgaaatc
      481 aacaatttgt tcagtaagtt taggatgttt cacgccgctt gcacgtcgca caaagcttac
      541 tacttccgtg taattgttat cttcgagcaa ttgccgggtt agttctgcgc cgatcatgcc
      601 ggtggaacct attattaatg ctttcttcat tattctggtt taaatttcct gatcaatatg
      661 tatatccctg aagccgagga agtagagaat tccatccagt ccgatggttg atatggattg
      721 actggcactt tccttcacct tgggcttggc gtgaaaggca atacccagcc cggccagatt
      781 caacatgggc aaatcgttgg ctccgtcacc cacagcaatg acctgttcaa gttgtatttt
      841 ttccacctga gcaatcagtt tcaggagttc ggcttttcgt tttccgtcca ccacatcgcc
      901 cagatagcga ccggtgagtt ttccatcggc tatttccagt tcattggcat agacataatc
      961 cacgtcaaag cgttgcttca ggtaattgcc gaaataggta aatcctccgg agagaatggc
     1021 aattttataa ccgcacattt tcaataccga catcaaccgg tcggcacctt ccatgatggg
     1081 cagactgcgg gctatttcct ccattacgct tacatccagt cccctgagca gggctacccg
     1141 ctgagcaaaa ctttcggtaa aatcaatttc gccgcgcatg gccgattcgg tgatggcccg
     1201 gacctgttcg cccacaccgg cacggtcagc cagttcgtct atcacttcgg tttttatcag
     1261 tgtggagtcc atgtcgaagc aaatcaggcg gcggttacgt ctgaagatat tatctttttg
     1321 aaaagaaata tcaatgcctt cgcgggcggt taggagcata aactgctccg aaagcagatt
     1381 tttatccttc agttcacccc taaccgacaa ttcaatgcat gaacggaggt tttcttcttg
     1441 ttcgtggagc gatgggcgcc ctgtgagacg cttgatggag tcgatattca gttcctgtaa
     1501 agccagttcg cgcgtaacca tggccagttg tcgtgcaccg atttctttgc ccagtatggt
     1561 gacaatgtag cggcttttac cctgccggtt cacccagttt tcgtagttct cttcgcttac
     1621 gggtttgaat cgtacatgca tattcagttc gttgcatttg aaaagcactt ctttcagaat
     1681 gttgcccgaa ttgctgtcat cgggagtttt gaaaagtatg ccgagcgaaa ggctgtggtg
     1741 aatatcagcc tgaccgatat ccaggatggt ggcgttgtta ttccccaaga tattggttat
     1801 ggcgttggtt actccgggtt tgtcttcacc cgatatagta ataagaatta tttcactgtt
     1861 catgataacg ctttttttct taaatgtaat taatcaactg ttttaccgga ttcccatata
     1921 tttattataa gtatcaagca cgttgggaac atagcgtatg ggttctttgg cccgaaaaaa
     1981 tccatatttg actaccggat cgttgtaaaa ttccggatcg ctttttttgg ccagataata
     2041 ttccacactg tcgtaccaca catgcggatt tttgccgtat ttttcggcaa gtgccatcgc
     2101 atccagtata tgtgcgggac cgcagttata gctggccagt atgaatttta tccgttcctc
     2161 tttatcggca atttttcggt aaatcatatt caaactttta atgtattcca cgcctgctgc
     2221 aatatttttc tccggatttt caatttcgta ctgatcgacc ccgaagtcaa gggcagtctg
     2281 aggcatcaac tgcataatac ctttggcacc ggcccacgaa actacggtgc tgtcgtagcc
     2341 cgactctacg aaagctaatg ctgccagtaa ttgccagtcc cagccgatat ctttagcgtg
     2401 ttttttgaaa atattgtcgt agggtgatat ggaacctttg ggaattggta tttttttgaa
     2461 agcaaaatat ggatttttct cccaatattt cccgtaaagt ttttctttca gatagttggt
     2521 ggcatccagc ttactccatg ccccgatgga gtctctcagt gcgctgtcat ctttgcgcac
     2581 gagccagccg ttgcgctgac tgaacccgat gggaatgcgg caatccagtg tacggttgaa
     2641 tattttttgc agtaatgctt tattgcggta tgctatggtg tattttattt tatttgcagc
     2701 cacctgaagc atcagatcat cggtattgag tgtgtcgttg gttttgatta tgatgcctcc
     2761 gcctatttct tcgttcaggg cttcgagccg ctcgtgatca atgctgtgtt tttttaccca
     2821 tacctctttc cctgtcagct ctatggggtc cgacaccgca tcgcgtccca tcatttgaac
     2881 caatacgagg tacgaatcgt catgcgcgaa aacaaaatta aattcctgtt tcaatgcctt
     2941 ggtttcgtag gtagtataaa tggccagatc ggcttttccg gtgcggagat agtcggtcat
     3001 ttcccggtca ttttttgcaa cttccacttt cagggtcaaa tgcaggtgtg cggccaggtt
     3061 ttgggccagt tcgtaatcgt aacccagaac ttcgtcacgg taaatgaagt atgatgtggc
     3121 tccgtactga gttacaacat gcagtgtatc tttcgggaaa atattttttt gaagggcttt
     3181 accatctgtc acagcatatt tttcgggttc gttggttgat ttacagctca tcaacaaacc
     3241 tgatataaac agtaaggcta tccaaaattt acatttcata cccggattaa atgtgttgta
     3301 cgttttcgga gctaatgatt tcgtgtccgt tcattctgag ccagacttgc gccgttgcca
     3361 gtacatcctt ctggcaatag gctgcgatgc ggggcaggtt attttccttg taatacacac
     3421 gtgccacctg actgccgtca atatcatcct tgggcgatgg aattccaaat acagctgtga
     3481 gcaatctgag cgaagtataa tttttataat caccgaattt ccaatagtcc agggtatcca
     3541 tcagatttac ctcccagggt ttgcggttgt ttatttgcag ctgcaggggc atgggcagat
     3601 tatttatcag catcctgcgg caaatgtagg gaatgtcaaa ctcctttata ttatgtccgc
     3661 aaaagttata atcgggatat ttttccaata actggctgaa gtctgcaagt acgcggattt
     3721 cgtcatcatc agcaaaagat ttggtgcgga aaaaccgttc attgttttta gtaaaaagaa
     3781 accccaccga aatgcagata atccgtccaa attcggcata aatcccggca ctgttttcga
     3841 atgcctgagc cggcgtagta tagtcgttgt acttttcggg catccgtttt tgcaacgatc
     3901 tgaatttttc atcccacaag tgctgtagtt cttcgttcat ttcggagtag caactcgtca
     3961 ttggcaccgt ttctatgtcg ataaacatga ttttctgaag cagtgggttc atatttggtg
     4021 tttttctaaa ataatttata aaactacggg atgagacaat ctgttttcag tggtgtagaa
     4081 caaactatcg ctctatttct tgttcagatt attttcaatg tatttggtca gaatgtcaat
     4141 agcaatatga ttgttgcctc cctgcggtat gatcaaatcg gcatagcgtt tggtgggttc
     4201 gataaactgc tgatgcatcg gttttacggt ctgttcgtag cgttccatca ctttatttac
     4261 cgatcggccc cgctctacga tgtcgcggtt gatgacgcgt atgaggcggt cgtcggggtc
     4321 ggcatccaca aacactttga g
//
LOCUS       DAOPZK010000156         4337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_26252_length_4337_cov_22.762961, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000156 DAOPZK010000000
VERSION     DAOPZK010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26252_length_4337_cov_22.762961"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /locus_tag="PKH68_08160"
     CDS             complement(<1..1950)
                     /locus_tag="PKH68_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNX12950.1"
                     /translation="MKNTRNLKSVIAGLVVLYGTLSVSVANAQTVWLDQLDLSTITQG
                     YGTPKSNKSIDGNTLTIAGKTFERGIGTHSESLLTVLLDGQATLFTAFVGIDDEVKGQ
                     HPAAEFIINGDGKMLWSSGIMRLGDAAKPCSVRLEGVKKLELLVTDGGNGNYYDHVNW
                     VDARFETTGATTFKTFNPVPGEPYILTPTPSASPKITGARVFGVRPGSPFQFLVTATG
                     DRPMTFSASKLPKGLKIDAKTGIITGKLKQRGTYNVVLKAKNAKGTAVRKFRIECGDR
                     IALTPPMGWNSWNCFAQEVSAEKVKRAADAMVSSGLINHGWTYVNIDDFWENHRDSKD
                     PSLRGEFRDEAGNIVTNKRFPDMKGLADYVHNLGLKIGLYSSPGPWTCGGCAGSYGYE
                     KQDAESYANWGFDYLKYDWCSYGNVIDGLPENDPLKVSSLSYNGGSELYLAMKPYKMM
                     GEILRQQPRDIVFSVCQYGMSDVWKWGDAVYGNCWRTTNDITDTWASVKNIALDQDKS
                     APYAKPGNWNDPDMLVVGHVGWGNPHPSKLKPDEQYLHISLWSLFAAPLLIGCDMEKL
                     DDFTLNLLTNDEVIEINQDPLGKQATCLQTIGELRIYVKELEDGSKAVGFCNFGLEIA
                     ELSYTDFAKLGLSGKYNVRDLWRQKD"
     gene            complement(2301..2795)
                     /locus_tag="PKH68_08165"
     CDS             complement(2301..2795)
                     /locus_tag="PKH68_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12951.1"
                     /translation="MGQYKANATNSHNFTLRFDYQKVGELVYKKWYSFSAEIQLTDGT
                     KYQLEPTGFWDSKIELKDDTQTLLEFKMGWKGIIIKTLFNDSEEVFLLKLKGLLSNKF
                     VLLDTNDEELMAAETSFKWSKLNFDYNIETTQVFDKFENKELLLLTILHCINYYITIV
                     AAAS"
     gene            complement(2820..3536)
                     /locus_tag="PKH68_08170"
     CDS             complement(2820..3536)
                     /locus_tag="PKH68_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNX12952.1"
                     /translation="MKNAIIIGATSGIGKELARLLVDDGFKVGITGRRTELLESLKSE
                     NPASYFIKAFDVKDTKTAEEKLEELTTELGGLDLVILSSGTGDINDKLDFEIEFRTIE
                     TNVIGWTFIADWTFKYFEKQKYGHLTAISSIGGLRGSRQSTSYNATKAYQINYLEGLR
                     QKATKLKEQIFVSDIRPGLVNTEMAKGEGLFWVMPVEKTARQIYKAIKKKKKIAYVTK
                     RWKLIASIVKRIPVQLYDRM"
     gene            complement(3579..4304)
                     /locus_tag="PKH68_08175"
     CDS             complement(3579..4304)
                     /locus_tag="PKH68_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HNX12953.1"
                     /translation="MKKKIQKVQLLSFSAFILLVLLSSCSRTFCIKKDSDKIEGVRDG
                     FRYELWDQNSQGKACMKLGKGALFNGQWSNVENYLARRGLGYNQTQEHGEIGNFYATY
                     NCSYMPSSEAGGNSYLSVYGWTVDPLIEYYIVEDWRNWIPSMSKGAVFKGTLEVDGSI
                     YDIYECTRINQPSIKGITTFSQYFSIRRDKRDKGAINISEHFNKWESLGLNMGKMHEV
                     SFVVEGYRNSGKFRFNALDIFVK"
BASE COUNT         1122 a          971 c          806 g         1438 t
ORIGIN      
        1 atctttctga cgccaaaggt cacgaacatt gtatttcccg cttaggccca attttgcaaa
       61 atcggtgtac gaaagttcgg ctatttcgag accaaagtta caaaaaccaa ccgctttact
      121 gccatcttcg agctctttta cataaatacg caattctcca atggtctgaa ggcaggttgc
      181 ctgctttccg agcggatcct gattgatttc gataacttcg tcgttggtca acaaattcaa
      241 cgtaaagtca tccagttttt ccatgtcgca accaatcaaa agtggtgctg caaacaaact
      301 ccagagactg atgtgcaggt attgttcatc gggtttcagc ttgcttggat gtggatttcc
      361 ccagcctaca tgaccgacca caagcatatc ggggtcgttc cagttgccgg gtttggcata
      421 aggagccgat ttgtcctgat cgagtgctat gtttttcacg cttgcccatg tatcggtaat
      481 gtcgttggta gttcgccagc aattgccata tactgcgtcg ccccatttcc aaacatccga
      541 catgccgtac tggcacacgc taaacacaat atcgcggggt tgctgacgaa gaatttcacc
      601 catcatctta tatggtttca tggccagata tagctcactt ccaccgttgt acgacagtga
      661 ggatactttc agaggatcgt tttcaggcaa accatcaatt acatttccat agctgcacca
      721 gtcgtatttc aggtaatcaa atccccagtt ggcataactt tcggcatcct gtttttcgta
      781 gccgtaactg ccggcacatc caccgcaggt ccacggtccg ggactggagt aaagcccgat
      841 tttcagaccc agattatgta cgtaatctgc cagccctttc atatccggaa aacgcttgtt
      901 ggtaacaatg ttgcctgctt catctctaaa ttctccacgc agggacgggt ctttcgagtc
      961 gcggtggttt tcccagaagt cgtcgatatt cacgtaagtc cagccgtgat tgattagtcc
     1021 gctgcttacc attgcatcgg ctgcacgttt cactttttcg gccgaaactt cctgagcaaa
     1081 acagttccag ctattccatc ccatgggagg agtaagggca atgcggtcac cacattcgat
     1141 gcggaacttt cttacggctg taccttttgc attttttgct ttcagcacaa cgttataagt
     1201 tccgcgctgc ttaagtttgc cggtgatgat gcctgtttta gcatctattt ttaatccttt
     1261 tggtaatttt gaagccgaaa aagtcatcgg tcggtcgccg gtcgccgtta caagaaactg
     1321 gaaaggcgaa cccggacgta ctccaaatac cctggcccct gtgatttttg gagaggccga
     1381 aggtgttggg gtcagaatat agggttcacc gggcaccgga ttgaatgttt tgaaagtagt
     1441 ggcgccggta gtttcgaacc tggcatccac ccaattcaca tggtcgtaat aatttccatt
     1501 tccgccatcg gtaaccagga gctccagttt tttcacgccc tccagtctta ccgaacaggg
     1561 tttggcagca tcgcccaagc gcattatgcc actggaccat aacattttac catcaccgtt
     1621 aataatgaat tcagcagcag gatgttgccc cttcacttcg tcgtcgatgc caacaaatgc
     1681 cgtgaacaag gtggcttgcc cgtcgagtag aacggttagc aaactttcgg aatgagttcc
     1741 gatgccccgt tcgaaagttt ttccggcaat ggtcagcgta tttccatcga ttgatttatt
     1801 gctctttgga gttccatatc cctgagtgat ggtgctgagg tctaactgat caagccacac
     1861 ggtttgagca ttcgcaacac ttacactcaa cgtgccgtac aaaacaacaa gtccggcaat
     1921 tactgatttt aaatttcttg tatttttcat ctgtttataa tttaaatatc tgccccctaa
     1981 tccgtcagct gacggaggaa tattgagcgt ctatcatttg ttgtaagttt tttaatcatg
     2041 ccagtgtgtg tcaaaaatat tttacttgga catgtgtgtt tcattgtatc attatggatt
     2101 actacggttt tatcagaaaa agtcatctgg acttttgatt aaactaatta atttattttt
     2161 ttactacgaa ccagcctgcg gttggcaggt aacactaatt acacgaaaat aattagtttt
     2221 attcgtgaat tgaaaatcga cgtaatcaaa ttagggtcat tcgtagctta ttaatataag
     2281 ctcaagatgc atttattgta ttatgacgct gccgcaacaa tcgttatata ataatttatg
     2341 caatgcaaaa ttgttaataa aagcaattct ttattttcaa acttgtcaaa aacctgggtt
     2401 gtttctatat tgtagtcaaa gtttaattta ctccatttga aactcgtctc tgctgccatt
     2461 aattcttcat cattcgtgtc aagcagtaca aatttattac tcaacagtcc ttttaatttt
     2521 aataaaaaga cttcttcgct gtcgttgaac aatgttttaa taattatccc tttccatccc
     2581 attttaaact ctaataaggt ttgggtgtca tcttttaatt caatttttga gtcccaaaat
     2641 cctgttggtt caagttgata ttttgtgccg tcagtcaact gtatttcagc actgaatgaa
     2701 taccattttt tgtaaaccaa ttctccaact ttttgatagt cgaatcttaa agtgaagttg
     2761 tgtgaattgg ttgcatttgc tttgtattgt cccatattga tgatatttct ttgttttggt
     2821 tacattctgt catataattg aacgggtatt cgtttcacaa ttgatgcaat tagtttccag
     2881 cgttttgtta catacgcaat tttctttttc tttttgattg ccttataaat ttgtcgggca
     2941 gtcttttcta cgggcattac ccaaaaaagt ccttcacctt tagccatttc ggtattgaca
     3001 agtcccgggc gaatgtctga aacgaaaatt tgttctttga gtttcgttgc tttttgtctt
     3061 aacccttcca aataatttat ctgatatgct tttgtagcat tataagaagt ggattgtctg
     3121 cttccacgaa gtccaccgat agaacttatc gctgtcaaat gtccgtattt ctgtttttca
     3181 aagtatttaa aagtccagtc agcaataaaa gtccacccga ttacatttgt ttcaatggta
     3241 cgaaattcaa tttcaaagtc taatttatcg ttaatgtctc ctgttcctga actcaaaata
     3301 actaagtcaa gtccgccaag ttcagtagtt agttcttcca atttctcttc cgctgttttt
     3361 gtgtctttca cgtcaaaagc ctttatgaaa taggaggccg ggttttccga tttcaaactt
     3421 tcaagcaatt cagttcgtct gccggttatt ccaaccttaa atccgtcgtc aacaagtaat
     3481 cttgcgagtt cttttcctat tccggaagtt gctccaatta tgattgcatt tttcatttaa
     3541 gctgtctgtc tttataacat ctgcgtttac tcactctgtt attttacaaa aatatccaag
     3601 gcattaaaac ggaattttcc actattacga taaccctcta caacaaacga aacttcgtgc
     3661 attttcccca tattcaagcc caatgattcc catttgttaa aatgttccga gatgttgatt
     3721 gctcctttat cccgtttatc tcttcgtatg ctgaagtact gcgaaaatgt agttatgcct
     3781 ttaatagaag gttgatttat tcgggtacat tcataaatat cgtaaatact tccatcgacc
     3841 tctaacgttc ctttgaaaac tgcgccttta ctcatagacg gtatccagtt tcgccagtct
     3901 tctacgatat agtattctat taacgggtcc acagtccagc cgtataccga cagatacgaa
     3961 ttgccgccag cctctgacga aggcatgtag ctgcagttat aagttgcata aaagttcccg
     4021 atttccccgt gttcctgagt ttgattataa cctaagcccc gacgtgccag ataattttcc
     4081 acattactcc attgcccgtt aaacaaagct cctttcccga gtttcataca ggcttttccc
     4141 tgtgagtttt gatcccataa ctcatagcga aaaccatcgc gcactccttc aattttatcg
     4201 gaatcctttt ttatgcaaaa tgttcgggaa cacgaactta aaagaaccaa tagaatgaat
     4261 gctgaaaagg ataataattg tactttttga attttttttt tcatgtgttt ataatttttt
     4321 tagcggcccc ctaaccc
//
LOCUS       DAOPZK010000157         4205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_27514_length_4205_cov_22.133976, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000157 DAOPZK010000000
VERSION     DAOPZK010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27514_length_4205_cov_22.133976"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="PKH68_08180"
     CDS             complement(<1..1895)
                     /locus_tag="PKH68_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNX12954.1"
                     /translation="MKRSLCYIVYFFLLVMGLQAQHAPDFVLKTNETGAFELVAPATK
                     FSVLADENDFTGVNFALKNLLTDFERVSGTKPTLSHSLSAENIKLIVGTVGKSTYVDQ
                     LVKDGKIDKNDLHGKREKFIIQTLENPFKETDKALVIAGSDMRGTIYGIYELSRQMGV
                     SPWYYWADVPVVKSEKLYIKTGKYTAGEPAVRYRGIFLNDEAPALSGWSKATFGGFNH
                     LFYEKVFELILRLKGNFLWPAMWGSAFYDDDPENGALADEMGIVIGTSHHEPMGRAHA
                     EWKKYGTGKWDYSKNEKVLQKFWKTGFERIKNYETVVTLGMRGDGDEPMSEDANIKLL
                     EKIVKDQRNTIKKVSGKKITETPQVWALYKEVQDYYDKGMRVPEDVTLLLCDDNWGNV
                     RKLPDLNAKPHKGGYGMYYHFDYVGGPRNYKWINVSQIQRVWEQMNLTYSHGVDRIWI
                     VNVGDLKPMEYPISFFLDMAWKPAQFNSQNLLQHTEKWCSEQFGEKNAKEAARIINLY
                     TKYNRRVTPELLNDTIFSLTNYNEFERVTNDYRSLALDAYRLFQQIPQNARDAFDQLV
                     LFPADACSNLYEMYFAVAKNKQLAAYNNPEANRWADKVKECFDRDSLLTVHYNTEIAG
                     GKWAHMM"
     gene            complement(2070..4037)
                     /locus_tag="PKH68_08185"
     CDS             complement(2070..4037)
                     /locus_tag="PKH68_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNX12955.1"
                     /translation="MKKTNIKVLMGAMMMLFLSACSDILDEQPRATYEPGFFTTKEGV
                     QSGLTSMYAHLRYIYGQGYYYNITLTGTDEYTWGNSADGNFKDADMTSGGTMTPSTSR
                     ADVLWNAAFPNINTASGVIENAEAVGLAPSLIAEARFFRAFDYFQLVQTFGGVPLDLG
                     GGIMKFNKSTVRTSVRNTVPEVYTKGIFPDLKQAITDLPETPRIVGAATKTVARLYLA
                     KAYLTYAWWLQNPNNIPTYPTTARTDPDGHDAAWYFQQAYDVATEAIVNPGYAGLQST
                     YYKVHLAQNDRNNEVLLLADHTNASEQYNGGISFSYGNGEAPDNFACWMVTPQMENLR
                     SGKKADGSDVISSVQREAAQWGGRPWTRMAPTQGVFTKTFADKTNDSRFDGTFTSVYR
                     GNWTKNSSMSSITTLYNANNMPVSPGDAILSFLPDESLTSTLSYTVGKSNIGAGVVAG
                     RADYVIPPSGINRQYFPGLWKIGTYRTDNGTGLGTPNGSTCRPFVIAKFSELFLVAAE
                     AAVKGATTKAITGTYANDGTARGLVNVLRARAGKWSFSNAENKAKVEDHSAEMIAATP
                     ANIDINYILAERSREFFGEGYRWFDLVRTQKWEEIAGTYEIAETGSHTPKTFTRTINK
                     AVHYLRPIPQGQIDRLDMSDDEKKAYQNPGY"
BASE COUNT         1135 a          939 c          847 g         1284 t
ORIGIN      
        1 tccatcatgt gcgcccattt ccctccggca atttcggtat tgtaatgcac agtcagcagc
       61 gagtcacggt cgaaacattc cttgacttta tctgcccagc gattagcttc gggattgttg
      121 tatgcagcca gttgtttatt ctttgctacc gcaaaataca tttcgtacaa attggagcag
      181 gcatccgccg gaaacaaaac cagttgatca aaagcatctc tggcattttg gggaatttgc
      241 tgaaataagc ggtatgcatc gagtgccagc gaacggtaat cattggttac ccgttcgaat
      301 tcattataat tggtcaggct gaaaatggtg tcgttgagca gttcgggagt tacccgccgg
      361 ttgtatttgg tgtacagatt gattattcga gccgcttctt tggcgttttt ctcaccaaac
      421 tgttcggaac accatttttc ggtatgttgc aacaggtttt gagaattgaa ttgtgcgggt
      481 ttccaggcca tatcgaggaa aaaactgatc ggatattcca tcggtttgag gtcgcccacg
      541 tttactatcc agatacggtc cactccgtga ctgtaagtaa ggttcatttg ctcccatacg
      601 cgctggatct ggctcacgtt gatccattta taattgcgcg gaccgcctac gtaatcgaaa
      661 tgatagtaca ttccgtatcc gcctttgtgt ggtttagcat tcaggtcggg aagtttgcgc
      721 acatttcccc aattatcgtc gcaaaggagc agagtcacat cttcgggaac gcgcatgcct
      781 ttatcgtaat aatcctgaac ttctttgtat aaagcccaaa cctgtggagt ttcagttatt
      841 tttttacccg aaactttttt aatagtattc cgctgatctt ttactatttt ttcaagcagc
      901 tttatattgg catcttcgct catcggttcg tcaccatcgc cccgcatgcc cagcgtaacg
      961 acagtttcgt aattctttat tcgttcaaat cctgttttcc agaatttttg caaaactttt
     1021 tcatttttac tgtaatccca cttacccgta ccgtattttt tccattcggc atgagcgcgt
     1081 cccattggtt cgtggtgcga agttccaatc acaatgccca tttcatcagc cagtgctccg
     1141 ttttcaggat catcgtcata aaatgcactt ccccacatgg ccggccacag gaagtttcct
     1201 ttcaggcgaa ggattaattc aaatactttt tcgtagaaga gatgattgaa gccaccgaaa
     1261 gtggctttag accagcccga gagagccggt gcttcatcgt tgagaaaaat accgcgataa
     1321 cgcacggcgg gttcaccggc agtgtatttt ccggttttaa tatacaattt ttctgatttt
     1381 actaccggaa catcggccca gtaataccag ggtgaaacgc ccatctgacg ggaaagttcg
     1441 tagatgccgt aaatggttcc gcgcatatcg cttccggcaa tcaccagggc tttatccgtt
     1501 tctttaaaag gattttcaag ggtttgaata atgaactttt cacgcttacc gtggaggtca
     1561 tttttatcaa ttttcccgtc cttaaccaac tgatcaacat acgtgctttt tccaaccgta
     1621 ccgacaatca gtttaatatt ttcagcagac agactgtgtg ataaggttgg ttttgtacca
     1681 cttactcttt caaaatcagt cagcaggttt ttcagcgcaa aattcacacc cgtaaaatca
     1741 ttttcatccg ccagaacaga aaatttagtg gcaggagcaa caagctcaaa tgcaccggtt
     1801 tcgtttgtct tcaaaacgaa atcaggcgca tgttgagctt gaagacccat caccaacaaa
     1861 aagaaataaa caatataaca caacgacctc ttcattaaaa cactattttt taattatatg
     1921 cgaaaaaact taattgtaaa ttttaattca attccatgaa aatctatatt aaaacatgaa
     1981 gattagatgt gtctttttta tctcagaaaa aaaacaaata attaagaaga taatgccttg
     2041 aagtcggcat tatcttctta atatcagtat taataacccg gattctgata tgctttcttt
     2101 tcatcatcgc tcatatccag tctgtcgatc tgaccttgag gaattggacg cagataatga
     2161 acagctttat taatggtacg ggtgaatgtt ttaggagtgt gagaaccagt ttcagcaatt
     2221 tcgtacgttc cagctatttc ttcccatttt tgagtgcgaa ccaggtcgaa ccagcgatag
     2281 ccttcgccaa aaaactcgcg tgaccgttca gccagaatat aattgatatc tatgttagcc
     2341 ggagttgctg caatcatttc agcgctgtga tcttcaactt ttgctttatt ttcggcattg
     2401 ctgaagctcc atttacctgc tcttgcacga agtacattca ctaatcctct tgcggtacca
     2461 tcattagcat aagttcctgt tatggcttta gtggtagctc ctttcacagc agcttcagca
     2521 gctaccagga aaagttcaga gaatttagca atgacaaacg gacgacaggt acttccattc
     2581 ggagttccta atcccgtgcc attgtctgta cgatatgtac cgattttcca caatccgggg
     2641 aaatactgac ggttaatacc agatggagga attacataat ctgctcttcc ggcaacaaca
     2701 cctgcaccga tattactttt accaacggta tagctaagtg tacttgtcag cgattcatcc
     2761 ggtaaaaatg aaaggatggc atcgcccgga gaaacaggca tattgttagc attatacaac
     2821 gtagtaatgg atgacatact tgaattcttt gtccagttac cacggtaaac gcttgtgaaa
     2881 gtaccatcaa aacgtgagtc gttggtttta tcggcaaatg tttttgtaaa cacaccctga
     2941 gttggcgcca tacgtgtcca tggacgaccg ccccattgtg ctgcttcgcg ttgtacagag
     3001 ctaataacat ctgagccgtc agctttctta ccgcttctta aattctccat ctgaggtgta
     3061 accatccaac aagcaaaatt gtcaggcgct tcaccgttac catacgaaaa actgattcca
     3121 ccgttatatt gctcgctggc gtttgtatga tctgcaagta ataaaacttc attattgcgg
     3181 tcattttgtg ctaagtgaac tttataatag gttgattgta aacctgcata tcccggatta
     3241 acaatagctt cggtagctac atcataagct tgctgaaaat accatgctgc atcatgacca
     3301 tcggggtctg tacgtgctgt agtgggatat gttggaatat tgtttgggtt ttgcaaccac
     3361 caggcataag taagataagc ctttgccaga tacagacgtg caacagtttt tgtggctgca
     3421 ccaactatac gtggagtttc aggaagatcg gttattgctt gtttcaaatc agggaaaata
     3481 ccctttgtgt aaacttcggg tactgtatta cgcactgacg ttcttactgt tgatttgttg
     3541 aatttcataa ttcctccacc taaatcaagt ggtactccgc caaaagtctg taccaattgg
     3601 aaatagtcaa atgcacggaa gaaacgagct tcggcaatca atgaaggtgc cagacctacc
     3661 gcttccgcat tttcgataac accgcttgca gtatttatat tcgggaaagc agcattccaa
     3721 agcacgtcgg cgcgagaggt ggatggtgtc atagttccac cactggtcat atctgcatct
     3781 ttaaagttcc catcagcact attgccccag gtatattcat cggtacctgt tagtgttatg
     3841 ttataataat acccttgccc gtaaatgtaa cgcaggtgag cgtacataga agtcaaaccg
     3901 ctctgaactc cttccttggt tgtaaaaaaa ccgggttcat aagtggcacg cggctgttca
     3961 tccaagatat cgctacaggc tgacaggaac aacatcatca tagctcccat caaaactttt
     4021 atatttgtct ttttcattat tatctttatt tagaggttat taaaatgtta agcttacgcc
     4081 aaccataaaa ttacgtgtag caggtgagtt agtacctatg gtaaggatac gtctgttagc
     4141 taagcctcct gctgaagcca tgttttcatc accgtaagag tttgtttcgg gatccatacc
     4201 tgttt
//
LOCUS       DAOPZK010000158         4176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_27780_length_4176_cov_37.225916, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000158 DAOPZK010000000
VERSION     DAOPZK010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27780_length_4176_cov_37.225916"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKH68_08190"
     CDS             complement(<1..720)
                     /locus_tag="PKH68_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNX12956.1"
                     /translation="MRKKLSLLSLLMLLANLIAAQNSPNITRVLEYRPAPGQHINRIY
                     PTPAMSDTPENALKFAENSLIDNKSMLGLGGFGGYVIVGFDHPIVNVKGEYDFKGLGN
                     AYTNSCEPGIVMVCQDLNKNGRPDADEPWYELAGSEYNNAATIHNYEITYYRPEPDGL
                     KSNIRWTDNQGKEGVIKHIPFASQSTMYPLWITDNTLKFKGTKLANTVVEVSGFYKLP
                     GFNWGYIDNQSNAETIDKNGFK"
     gene            complement(723..1811)
                     /locus_tag="PKH68_08195"
     CDS             complement(723..1811)
                     /locus_tag="PKH68_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12957.1"
                     /translation="MKTFRTIILLLTALFIYSCDDMHDVERNDGLPLTQSSGRMLVLS
                     EGLFNMNNSTLAYYNFDTKEFNTNYFQTKNKRGLGDTANDMILYGSKLYIAVDISSQI
                     EVVDVNTGISLKQIPLFNDKNSSRQPRYLESSDGKVFVSCFDGTLVRIDTAKMVVDGT
                     VSCGRNPEGLCIAAGKIYVANSGGLDFPKYDNTVSVIDMKTFKELKKITVGTNPYIIH
                     SDSQGDVYVSSRGNYGENPYRFQKIDTYIDEVVQDFEGLNVLNFTIYNDKAYLYNYDF
                     TTNKNWVKVFDCLSEKVVNAGFISDNTVLDTPYSIQVNPFDGDVYITNSPGYTLWGDV
                     LCFDKTGKLKFRINEIGLNPNKIVFLNE"
     gene            complement(1822..3873)
                     /locus_tag="PKH68_08200"
     CDS             complement(1822..3873)
                     /locus_tag="PKH68_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX12958.1"
                     /translation="MISANFKHILSFTGISLLSGIFMTVYGQNPPDQKKDTTRVHYIQ
                     EIVVTESTRNAEIRSSTPLQILSSKSLMELNALQVSDAVKQFSGVTVKDYGGIGGLKT
                     VSVRSLGANHTTVSYDGIALTDVQSGQIDIGRFSLENVDMISLSSGQSDNIFQPARLF
                     ASASVLNIQTLSPEFRDNRKMNGKVALKAGSFGLINPSLTLNRKFNQRLSASFSGEWL
                     AANGEYPYRLNYGFAGKDSSSMETRRNTDVNNLRLEAALHAAFSDKSRGYIKTYFYQS
                     ERGLPGATIYYNLTNFSTQRIWDNTFFTQAHYRHELSRKWEIQANAKYNRGYLRYLDP
                     VVLNSDGKTENTYVQNEIYGSVSTLFRAFENISFSASSDVSGNTLDAGGSGFTSPSRL
                     SWLTVLAGKYVTNRVLATASLLYTHINEQTKTGDAAENQRKFSPYISLSVKPFTTADL
                     RFRAFYKNIFRVPTFNDLYYSRVGNPNLKPENAQQFNLGATYTTTFNELLQFFSVTAD
                     AYHNRIDNKIVAYPTKNIFEWTMLNYGRVDIDGLDFSLEAAYRIAKGYNLIAGGSYTY
                     QRALNVTNPDDRDYNQQIPYTPRVSGSGRASLETPWINLNYSTIWSGHRYAVNQNYAE
                     NRVEGYSDHSISASRNFITSFGIIGAGIEVLNLTDKNYEVVRFFPMPGRSFRGNISLK
                     F"
     regulatory      complement(3981..4173)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1114 a          955 c          936 g         1171 t
ORIGIN      
        1 cttgaaacca tttttgtcga tcgtctcagc attcgactgg ttatcaatat atccccaatt
       61 aaagcccggt aatttgtaaa agccgctgac ttcaacaaca gtgttagcga gttttgttcc
      121 cttaaatttc agcgtattgt cggtaatcca gagcggatac atggtggatt gcgaagcaaa
      181 aggaatatgc ttgattacgc cttcttttcc ctgattgtcg gtccagcgga tgttcgattt
      241 cagtccgtcg ggttcgggac gataataggt gatttcgtag ttgtggatcg ttgccgcatt
      301 gttatattca gacccggcaa gttcgtacca gggttcatca gcatcgggtc gtccgttttt
      361 attcaaatcc tggcacacca tcacaattcc cggttcgcag ctgttggtgt acgcatttcc
      421 caatcctttg aagtcgtatt cacctttcac atttacgata gggtgatcaa agccaacgat
      481 tacataaccg ccaaaacctc ccagcccaag catcgattta ttgtcaatca gactattttc
      541 ggcaaatttc agggcattct ccggagtgtc actcatggcc ggagtggggt agatgcggtt
      601 gatatgctgg ccgggagccg gacgatattc cagcacacgg gtgatgttgg gtgagttttg
      661 tgctgcaatt aaatttgcaa gcaacatcag taaggataac aaggataatt tttttctcat
      721 aatcattcgt ttaaaaacac aatcttattt ggatttaacc cgatttcatt gatccggaat
      781 tttagttttc cggttttatc aaaacaaagc acatcacccc aaagcgtgta accgggtgag
      841 ttggtgatat aaacatcacc atcaaacgga ttaacctgaa tagaataagg ggtgtcaagc
      901 acggtattat cagaaataaa accggcgttg acaactttct cgctcagaca gtcgaatact
      961 ttcacccagt ttttattggt ggtgaaatcg taattataca aataggcttt atcgttgtaa
     1021 atagtgaaat tcagcacatt cagtccctca aaatcctgaa ccacttcgtc gatgtaggta
     1081 tcaatttttt gaaaccgata cggattttca ccgtaatttc cgcgcgacga aacatacacg
     1141 tcgccctgac tatcggaatg tatgatgtac ggatttgttc caaccgtgat ttttttcaat
     1201 tccttaaatg tcttcatatc aatcaccgaa accgtgttgt cgtatttcgg gaaatccagt
     1261 ccgcccgaat ttgccacgta gattttaccc gctgcaatgc aaagcccttc gggatttcgt
     1321 ccgcaactta ctgtgccgtc caccaccatt ttggcggtgt caatcctgac cagcgtgcca
     1381 tcaaagcagc tgacaaatac ttttccatca ctgctttcca gatagcgcgg ctgacgagaa
     1441 gaatttttat cgttaaacag cgggatttgt ttcaacgaaa tgcccgtgtt gacatccacc
     1501 acttctatct gactggaaat atccaccgca atgtataatt tagaaccgta taatatcata
     1561 tcgttggcag tatcgcccaa cccgcgcttg ttttttgtct gaaaataatt tgtattaaat
     1621 tcttttgtgt cgaaattata atacgccagc gtgctgttat tcatgttgaa taatccttcc
     1681 gaaagtacga gcatcctgcc ggaactttgc gttaggggta agccgtcatt tcgttcaaca
     1741 tcgtgcatgt cgtcgcacga atagataaaa agcgcggtta acaacagtat gattgttctg
     1801 aatgttttca ttttccaaaa attaaaattt caacgaaata ttccccctga acgaccttcc
     1861 cggcatgggg aaaaaccgaa ccacttcgta attcttatcg gtcaggttaa ggacctcgat
     1921 accggcacca attattccaa aacttgttat aaagttacgc gatgcagaaa tgctgtgatc
     1981 cgaatagcct tccaccctgt tctcggcata attctggtta acggcgtatc ggtgtcccga
     2041 ccagatagtg gaataattca ggtttatcca cggagtttcc aggcttgccc tgcccgaacc
     2101 ggaaacgcgc ggtgtgtatg gaatttgctg gttgtaatcg cggtcatcgg ggtttgttac
     2161 gtttaatgcc cgttgataag tgtacgaacc gcccgcaatc aggttatacc cttttgcaat
     2221 cctgtaagcc gcttccagtg aaaaatccag tccgtcgata tcaacccggc cgtaattaag
     2281 catggtccac tcgaagatgt ttttggtggg ataagccaca attttgttgt caatccggtt
     2341 gtgataggca tccgccgtta ccgaaaaaaa ttgcaataac tcattgaaag tggtggtgta
     2401 agtcgccccc agattgaact gttgggcatt ttcgggcttc aagttcggat tacctacgcg
     2461 tgaataatac aaatcattga aggtaggaac acggaagata ttcttgtaga atgcccgaaa
     2521 tcgcaaatcg gcggttgtaa atggtttcac cgataaactg atgtacggag aaaattttcg
     2581 ctgattttcg gcggcgtcgc ctgtcttcgt ctgctcgtta atgtgagtat aaagcaaact
     2641 cgcggtggcc agcacccgat tggtcacata ttttccggcc agcaccgtga gccagctcag
     2701 gcgggatgga gatgtaaaac cgcttccgcc ggcatcgagg gtattgcccg aaacatccga
     2761 agaagccgag aatgaaatat tttcaaaggc tctgaaaagc gtggaaacag aaccgtagat
     2821 ttcgttctgc acgtaggtgt tttcagtctt tccgtccgaa tttaaaacaa cgggatcgag
     2881 gtagcgcagg tatccgcggt tgtatttcgc gtttgcctga atttcccatt tccgcgaaag
     2941 ttcatgccgg taatgagcct gcgtgaaaaa cgtgttgtcc caaatgcgct gcgtggaaaa
     3001 gttggttaag ttgtagtaaa tggtcgcgcc cggcaatcca cgctccgact ggtaaaagta
     3061 agttttgata tagccccggc ttttatccga aaatgcagca tgcaaagcag cttccaggcg
     3121 gagattgttc acatcggtat tgcggcgggt ttccatcgac gagctgtctt ttcccgcgaa
     3181 accgtaattg agcctgtaag gatattcccc gttggcagcg agccactcac cgctgaaaga
     3241 ggcagaaagt cgttggttga atttccggtt gagcgtgaga gaaggattta tcaaaccgaa
     3301 tgaaccggct ttcagcgcga ctttgccatt cattttccga ttatcccgaa attcagggct
     3361 aagggtttgg atgttcaaaa cggatgccga cgcaaaaaga cgagccggtt gaaagatatt
     3421 atcactctga ccgctgctga gcgaaatcat atccacattt tccagcgaaa accgtccgat
     3481 gtcaatttgt ccgctctgaa catcagtgag cgctatgccg tcgtaactca ccgtggtatg
     3541 attagccccc agacttcgca ccgataccgt tttcagtccg ccgatgccgc cataatcctt
     3601 caccgtcacc cccgaaaact gtttcacggc atcagaaacc tgcaacgcat tgagttccat
     3661 cagcgatttg gaactgagta tctgcaaggg agtgctcgaa cgaatttcgg cattgcgggt
     3721 actttcagta accacaattt cctgaatgta atgcacgcgg gtggtatcct tcttctgatc
     3781 aggcggattt tgtccgtaga cggtcatgaa aattccggaa agcagtgaaa ttcccgtaaa
     3841 tgaaagaata tgtttgaaat tagctgaaat catacaatct atttgaaaat acaacaaata
     3901 gcttgcaaga aaagaaaatc aacgggcaca aatagatata gatatcggtg cgtaattggt
     3961 tatagctctg tcctcgaaag cgttaaacaa aatcatttgg caggtcttct gacttactcc
     4021 cgtaggtgaa cgagccttcc cgtcttatca ttcggagaca gtggcaaagt gttgttcatc
     4081 acgttgaaag agcttacagc agcgggactg ttgccgattt tcacgacatt cccttttaat
     4141 cgatttccgc ggaaggcgga aattcaaacc aaattt
//
LOCUS       DAOPZK010000159         4163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_27879_length_4163_cov_17.954966, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000159 DAOPZK010000000
VERSION     DAOPZK010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27879_length_4163_cov_17.954966"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2439)
                     /locus_tag="PKH68_08205"
     CDS             complement(<1..2439)
                     /locus_tag="PKH68_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNX12959.1"
                     /translation="MKKIQSFICIVSIIVIISFQKISSQEIWKDPSKSFELRTSDLVS
                     KLTLEEKVQLMRYDSPEISRLSIPAYNWWNECLHGVARSGLATVFPQAIGMAAMWDDR
                     MMFDIADAISDEARAKYNDYSHRGKRGIYQGLTFWTPNINIFRDPRWGRGMETYGEDP
                     FLTAGMAIPFIKGLQGNDPKYLKLVATAKHFAVHSGPEATRHSFDVWPNDYDLAETYF
                     PHFKRTVTEAGVYSVMCAYQRFKGIPCCGNQFLENELRNKWNFKGYIVSDCWAIKDFY
                     DPKAHHITNSPEEAAAMAVKAGTDLNCGVTYANSLLDAVKKGLVSENEIDTSVKRLLL
                     ARMKLGQFDPENEVPFSNIPLKVVDNEEHRRLALDAARKSIVLLENPNNLLPFTKNVK
                     RVAIIGPNADNHDVLLGNYNGYPSFETTPLKGIKEKLPNAEVKYSQGCKLADGLPCLT
                     IIPEKYFFTDKSLKNKGLKGEYFDNSDLKGKPKHKRTDANLNFSWWTTPPFPDMKYEH
                     FSVRWKGVLVPPVTGMYALGGEAMNGFRLYIDGKMLTFGKINEHHSRKDYEYVQLEAG
                     KKYKIQAEYVQDNSEYSHIRILWELPNPGLKKEALELAANSDLVVLCMGLSPMLEGEE
                     MKVKVKGFAGGDRLDINLPETQTELIRAIQKIGKPTVLVLLNGSAVAFNPDAENKPAV
                     LDAWYPGQSGGAAIADVIFGDYNPAGRLPVTFYKDINQIPAFEEYDMKGKTYRYFSGK
                     PLYEFGYGLSYTSFEYSVKNIPDEIITGEDIKLSVNVKNTGSMDGDEVVQLYVSLPDS
                     KLKKPIRSLQGFKRI"
     gene            complement(2571..>4163)
                     /locus_tag="PKH68_08210"
     CDS             complement(2571..>4163)
                     /locus_tag="PKH68_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNX12960.1"
                     /translation="TIGMRGDGDEAMSKETDVQLLERVVKNQRKIIEQVTKRPAKQTP
                     QIWALYKEVLDYYDAGMRVPNDVIMLLCDDNWGNVRRLPNEKERKHPGGWGLYYHVDY
                     VGAPRNTKWLNVTPIQNMWEQLQLTYDYGVDKLWVLNVGDIKPMEYPITLFMDMAWNP
                     TRYTAANLMEHPRAFCAQQFGDEQADEAMRILNLYSKYNGRVTAEMLDRNTYNLETGE
                     WKQVSDEYLKLEAEALRQYLDLKPEYKDAYKQLILFPVQAMANIYEMYYSQAMNHKLF
                     AENNPKANEWADNVERTFKRDAALSYDYNKVMADGKWDGMMIQKKIGYTIWNDNFPAD
                     KLPEVFRIENSDSAVGSYVFSPSNGYIAIEAEHYYSLINAANAKWTVIPYMGRTLSGI
                     SLQPYSQSVDGASLSYKMKLPEDVKKVTVHVVVKSTLAFSNLDGHRYKVGFNGAEEKT
                     INFNSDLNEKKENIYSIFYPTVARRVVEKKIDLNVPAVADGLQMLTLTPLDPGIVFEK
                     IVIDFGGYKNSYLFMNESNSKR"
BASE COUNT         1123 a          954 c          822 g         1264 t
ORIGIN      
        1 aattcttttg aaaccctgca acgaacgaat gggctttttg agtttgctgt ccggaagtga
       61 aacgtatagc tgcaccactt catcgccatc cattgaaccc gtattcttta cattcacgga
      121 taatttaata tcttcaccgg taattatttc atcgggaata tttttaacag aatattcaaa
      181 agacgtataa cttaatccat atccaaactc atacaacggc tttccactga aataccggta
      241 cgtttttccc ttcatatcgt actcttcgaa agccggaatt tgattgatat ctttgtaaaa
      301 agtcaccggc agacgtccgg caggattata atcgccaaaa ataacatcgg ctatcgctgc
      361 tccgcccgac tgacccggat accaggcatc gagaacagca ggcttgtttt ctgcatcagg
      421 attgaaagcc acagcgctgc cgttgagtaa aaccaacacg gtgggtttac cgattttctg
      481 aatagcccgg atgagttcag tctgcgtttc gggaaggttt atatccaggc ggtcgccacc
      541 ggcaaaacct ttcactttca ccttcatttc ctcgccttcg agcatcggac taagacccat
      601 gcagagtaca accaaatccg aattagcagc cagttcgagt gcttctttct tcaaaccggg
      661 gtttggcagt tcccataaaa tgcggatgtg cgaatactca ctgttgtcct gaacatattc
      721 tgcctgtatc ttatatttct taccggcttc cagctgaaca tactcataat ccttacgcga
      781 atgatgttcg ttaattttgc cgaaagtcag cattttgcca tctatataga gcctgaaacc
      841 attcatggct tcaccgccaa gagcgtacat tccggtgacc ggtggcacca gcaccccttt
      901 ccagcgaaca gagaaatgtt cgtatttcat atccggaaac ggaggagtag tccaccacga
      961 aaaattcaga tttgcatccg ttcgtttgtg ttttggcttg cctttcaaat cagaattgtc
     1021 aaaatactcc cctttcaatc ccttattttt cagcgatttg tctgtaaaga aatatttttc
     1081 aggaataatt gtcaaacagg gaagtccatc ggctaattta catccctgag aatatttcac
     1141 ttcggcattg ggcagttttt cttttatgcc tttcaagggt gtagtttcaa aggatggata
     1201 accattgtag tttccgagca aaacatcgtg attatctgca tttggtccga tgattgccac
     1261 tcgttttaca tttttagtaa aaggcagcag gttgtttgga ttttcgagca aaacgatgga
     1321 tttacgggca gcatcaagag ccagccgtcg atgctcttca ttgtcaacca ctttcaacgg
     1381 aatatttgaa aatggcactt cgttttcggg gtcaaactgt ccgagtttca tccgggcaag
     1441 cagcagtctt tttacggaag tatctatttc attttcacta accagtcctt tttttaccgc
     1501 atccagcaag gaattggcat aagtaacgcc gcaattgagg tctgtacctg cttttacagc
     1561 catggcggca gcctcttcgg gcgaattggt gatgtggtga gctttgggat cgtagaagtc
     1621 tttgatcgcc cagcaatcgg acacgatata tcctttgaaa ttccacttgt tacgcaattc
     1681 attttcaaga aactgattgc cgcaacaggg aattccctta aaacgctgat aggcgcacat
     1741 caccgaatat acgccggctt cggttaccgt gcgtttgaaa tgcggaaaat atgtttcagc
     1801 caggtcgtaa tcattaggcc atacatcaaa cgaatggcgg gtggcttctg gcccactgtg
     1861 aacagcaaaa tgttttgcgg tagctaccag tttcagatat ttcggatcat taccctgcaa
     1921 ccctttaata aacggaatag ccattccggc agtaagaaac gggtcttcgc cgtaggtttc
     1981 catgcctctg ccccagcgcg gatcacgaaa gatattgata tttggcgtcc agaaagtcag
     2041 tccctgataa attccccgtt tgccacgatg cgagtaatca ttgtatttag cgcgggcttc
     2101 gtccgaaatg gcatctgcaa tatcaaacat catccggtcg tcccacatgg cagccatccc
     2161 gatagcctgc ggaaaaaccg tagctagtcc cgaacgtgcc acaccatgca gacattcgtt
     2221 ccaccagttg taggcgggaa tgctcagccg actgatttca ggcgaatcat agcgcatcag
     2281 ctgcactttt tcttcaagtg taagtttcga aaccaaatcg ctggttctga gctcaaaact
     2341 tttcgaagga tctttccaaa tttcctgcga tgaaattttt tgaaaggata tgattacaat
     2401 aattgaaact atgcaaatga aactttgaat ctttttcata ctattttttt tgaacagaaa
     2461 aattactgac caaaattatc aatttctatt aaataaaact tcgataaaaa ctgctgaagt
     2521 ggtgtataat ctggtttttt tagtaaacca aatgcgttat atacttgaag ctatctttta
     2581 gaatttgatt cattcataaa cagatacgaa tttttatatc caccaaaatc aatgactatt
     2641 ttttcgaata caattccggg atctaagggc gttagcgtaa gcatctgcaa accatctgcc
     2701 acagcaggaa cattcaaatc tatttttttt tcaactacgc gacgggctac tgtcggataa
     2761 aatattgaat aaatgttttc ctttttttcg ttcaaatccg aattgaaatt gatcgttttt
     2821 tcttcagctc cattgaaacc aactttgtaa cgatgaccat caaggttcga aaatgccagt
     2881 gttgatttta caacaacatg tacggtaacc ttttttacat cttcgggaag tttcattttg
     2941 tacgaaaggg aagcaccatc aacagactgt gaataaggct gtaaggatat accacttaaa
     3001 gtgcgaccca tatacggtat aactgtccat ttggcattgg cagcattaat taatgagtaa
     3061 taatgctctg cttctattgc tatatatcca ttacttgggc tgaatacata actgcctact
     3121 gctgaatcag aattttcgat acggaagact tccggcaatt tatcggcagg aaaattatca
     3181 ttccaaatgg tatatccaat tttcttttga atcatcattc cgtcccattt gccatcagcc
     3241 atcaccttgt tataatcgta acttaacgct gcatcgcgct taaaagtacg ctccacatta
     3301 tcagcccatt cgttggcttt tggattgttt tcggcaaata gtttgtgatt catggcttgc
     3361 gaataataca tttcataaat atttgccata gcctgaaccg gaaacagaat taactgtttg
     3421 taagcatctt tgtactcagg tttcaagtcc agatactggc gcaatgcttc agcttcaagt
     3481 tttagatatt cgtccgaaac ctgtttccat tcgcctgttt cgaggttata ggtattccgg
     3541 tcgagcattt cggcagtcac acgtccgttg tatttgctgt acaggttgag aattcgcatt
     3601 gcttcgtcgg cttgttcgtc gccaaattgc tgcgcacaga aagcacgtgg atgttccatt
     3661 aaattagctg ctgtgtagcg ggtcgggttc caggccatgt ccataaaaag cgtaatagga
     3721 tattccattg gttttatatc gcccacattg agcacccaaa gtttgtccac accataatcg
     3781 taagtgagtt gcaactgctc ccacatattc tgaatgggag taacattcag ccatttggta
     3841 ttgcggggcg caccaacata gtccacatga tagtacaagc cccaacctcc gggatgtttt
     3901 ctttcctttt cgttaggtaa tcgacgtaca ttaccccagt tgtcgtcgca caaaagcata
     3961 attacatcat tgggaacgcg cattccggca tcgtaataat cgagcacttc tttgtagagc
     4021 gcccatattt gcggagtttg tttagccgga cgtttcgtaa cctgctcaat gattttgcgc
     4081 tgatttttca ccacccgttc aagcaattgc acatcggttt ctttcgacat tgcttcatcg
     4141 ccatcgccac gcataccaat ggt
//
LOCUS       DAOPZK010000160         4139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_28100_length_4139_cov_20.244368, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000160 DAOPZK010000000
VERSION     DAOPZK010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28100_length_4139_cov_20.244368"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1389)
                     /locus_tag="PKH68_08215"
     CDS             complement(<1..>1389)
                     /locus_tag="PKH68_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX12961.1"
                     /translation="YNFYDLNGKISHKFSDRSRLFLSIYAGNDKMSGHYTVDSKENRN
                     EPSEYNTTPYFIDNNSDQKVKIAWGTKLASLNWAYQLNSKLYSSSTLIFSQYNSDISS
                     SEKEAYSYAPKPETGLTEKKEAFSKSSVYGSGITDVGVRTDFDYTPVHNHYIRYGATL
                     INHFFRPEHTRVGALEDNDGNKLEKEFIYANDSVNVLETSLYAEDEMDISDKIKLNLG
                     VHLSNFNVEKKNYLSLQPRVSGRYLLSETLSFKASYAQMSQYLHLLQSSFLSLPNDLW
                     MPVTANVKPLVSHQLSGGVYWDYRKINFSMEGYYKTSKNQMEYKEGAELFSVGKNWEE
                     RVAQGNGKAYGVEWMAAKKQGKTTGWAGYALSWATRHFPNGEINGGKPYYAKFDNRHK
                     INIVVNHKFNRKVDVNASWFLATGNWLTLPLEKYSSSSYPQYYYSERNNYKMPPYHRL
                     DLSVNFTKQKKKG"
     assembly_gap    1390..1489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1490..2577)
                     /locus_tag="PKH68_08220"
     CDS             complement(<1490..2577)
                     /locus_tag="PKH68_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX12962.1"
                     /translation="MKRILLISFITVFYFGISAQNKLSFQFKNTPIVAALTEIEKQNG
                     FTFSYNPELLKNFPNVTAQFQNSSLKNVLAVLFDRTDIQYLINERYIILKKRPKATKH
                     RTISGYIYDRETTETLIGANISDPKSGNATVSNNYGFYSLTVPDEITELQASYIGYGT
                     HSFQIENNEKGPFNIFLKPSLALDTLVVKGSRVTNLRSTDIGKLSMNASELKSVPSFM
                     GESDLVKTLQLTPGVTAGTEGIAGMYVRGGNLDENLYLVDGNPVYHVNHMLGIFSTFN
                     TDAVKLMNFYKGSFPARFGGRLSSVVDMRMNDGDSKKIGGTVSVGLLSSRLNLNGPLF
                     SDKTTFNVSLRRTYFDLFTTPLIALINY"
     gene            complement(2577..3386)
                     /locus_tag="PKH68_08225"
     CDS             complement(2577..3386)
                     /locus_tag="PKH68_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNX12963.1"
                     /translation="MSNNENNLEKSVRFVAMHYKPGIFNPDESLEKITGKRKRFLLRP
                     AVWSGAAAAALILIIAGTIFLQPEPGKTLTADAFTTISVLPDETKITLQPGATLAYDK
                     HFGKEMRRVSLSGNASFEVSKDKEKPFIVHTSAAEITVLGTVFDVSADEKSTRLEVHS
                     GKVNFAPANFPVSFICTREMQAQFDTQQSELVVSSPESSCTINKTENRFTFSNLALGE
                     VCRILQQYYNQPVSLTENEKSIRLTSAFIDKNITEIIQIINLTLDTHIELN"
     gene            complement(3577..4086)
                     /locus_tag="PKH68_08230"
     CDS             complement(3577..4086)
                     /locus_tag="PKH68_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNX12964.1"
                     /translation="MSNPQRYFSTQFHNYYRPLCLFALRLLDNADEAEDVVQDTFAAL
                     WEKRDTLHKIVSIKSYLYTAVRNNCLMKLRSKKEFDSVEELQLMDEYTPEEVIARAEL
                     EAKLWKMIDELPERQREIFLMAKRDGMAYKEIAEETGLSVKTVENHVTRALKSLRQKD
                     PNTFLFFFA"
BASE COUNT         1041 a          872 c          863 g         1263 t
ORIGIN      
        1 ccttttttct tttgtttggt aaagttgaca cttaaatcca accggtgata cggcggcatt
       61 ttgtaattgt tccgttcgga ataatagtat tgcgggtacg agctgctcga atatttttcg
      121 agtggcagcg taagccagtt accggtggcc aaaaaccacg aagcatttac gtccactttc
      181 cggttgaatt tgtgattaac gacaatgttg attttatgac ggttatcaaa tttggcataa
      241 tacggttttc ctccgtttat ttcaccgttg gggaagtgac gtgtagccca cgaaagtgca
      301 tatcctgccc agccggtggt ttttccctgt tttttggcag ccatccattc cacaccgtac
      361 gcttttccgt taccctgagc cacccgctct tcccagtttt tccctaccga aaaaagttca
      421 gcgccttctt tgtattccat ctgatttttg gatgttttgt aatacccttc catcgaaaaa
      481 ttaatttttc ggtaatccca atacactcca cccgacagct gatgcgaaac aagcggtttg
      541 acattggcag taacgggcat ccaaaggtca ttgggcaaac tcagaaaact gctttgaagc
      601 aggtgcaggt attggctcat ttgagcgtat gaggctttga aagaaagagt ttcgcttagc
      661 aaatagcgtc ccgaaacacg cggctgcaag ctgaggtagt ttttcttctc cacattaaaa
      721 ttggaaaggt gaacgcccag gttcaatttt attttatcgg aaatatccat ttcatcttcc
      781 gcgtaaagtg atgtttccag cacatttaca ctatcattgg catagataaa ttccttttcc
      841 agtttattgc cgtcattatc ttccagcgct cccacccggg tatgttcggg acggaaaaaa
      901 tgatttataa gcgttgcacc atagcggatg taatgattgt gaacgggtgt gtaatcaaaa
      961 tctgttctca ctccaacatc ggtaattccc gaaccataaa cagatgattt agaaaatgct
     1021 tccttctttt cggtgagacc ggtctccggc ttgggtgcat agctgtaagc ttctttctcc
     1081 gatgaactga tgtcggaatt atactggctg aatatcagcg tactgcttga atagagcttg
     1141 ctgtttaact gatatgccca attgagtgaa gccagtttcg ttccccaggc aattttcact
     1201 ttctgatccg aattattgtc aataaaatag ggtgtggtat tgtattcact cggttcattc
     1261 ctgttctctt tgctgtcaac cgtgtaatgt ccgctcattt tatcgtttcc ggcgtatata
     1321 ctcagaaaaa gccggcttct gtccgagaat ttatgactga ttttcccgtt caggtcgtag
     1381 aagttgtagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tatagttgat
     1501 cagggctatc aacggagtgg taaacaggtc gaaatacgtg cgtcgcaagg aaacattgaa
     1561 ggttgttttg tcgctgaaaa gaggtccgtt caggttcaat cgcgaagaaa gtaatcccac
     1621 cgaaaccgta ccgccgattt tcttgctgtc gccgtcgttc atgcgcatat ccaccaccga
     1681 cgagagcctt ccgccgaaac gtgccggaaa acttcctttg taaaaattca tcagcttcac
     1741 agcatcggta ttgaacgtgg aaaaaattcc cagcatgtga ttaacgtgat ataccgggtt
     1801 tccatcaacc agatacaggt tttcatccag atttcctccg cgcacgtaca tacccgcaat
     1861 gccttcggtg ccagccgtta cgccgggtgt gagttgcagc gttttcacca gatcactttc
     1921 gcccataaat gaaggcaccg atttcagttc cgatgcattc atgctcaatt ttccaatgtc
     1981 ggtacttcgc aggttggtca cccggctccc tttcaccacc aacgtatcca gtgccagaga
     2041 tggtttcaga aaaatattga atggaccttt ttcattgttt tcaatctgaa aagagtgcgt
     2101 gccgtaacca atgtaacttg cctgcaattc agtaatttcg tcgggaactg tgaggctgta
     2161 aaatccgtaa ttgttgctca cggttgcatt gcccgacttt gggtctgaga tattggcacc
     2221 gataagcgtt tcagttgttt cgcggtcgta aatatagccg ctgatggttc ggtgtttcgt
     2281 tgcctttggc cgctttttca gaataatgta ccgttcgttg attaaatatt gaatatcagt
     2341 ccggtcaaat aaaacagcca gcacattttt cagtgagctg ttttgaaact gagctgttac
     2401 gttcggaaaa tttttcagta attccggatt atatgaaaat gtaaaaccgt tttgtttctc
     2461 aatttcagtc aatgcagcca caatgggtgt attcttaaac tgaaatgaca gcttgttttg
     2521 ggcagatatt ccgaaataaa atacggtgat gaacgaaatt agtaaaatgc gtttcattag
     2581 ttaagttcaa tatgtgtgtc gagcgtaaga ttgatgatct ggattatttc ggtgatattt
     2641 ttatcaataa aggcggatgt caaacgaatg cttttttcgt tttcggttaa actaaccggc
     2701 tgattatagt actgttgcaa tatccgacag acttcaccca gcgctaaatt gctgaacgta
     2761 aaccggtttt cagttttatt aatcgtgcaa ctgctttcgg gtgatgaaac gacaagttct
     2821 gattgctgag tgtcaaactg tgcctgcatt tcgcgggtac agataaaaga aaccggaaag
     2881 ttggcgggtg caaaattcac ttttccggag tgaacttcca gtcgggttga tttttcgtca
     2941 gctgatacat cgaaaacagt tcccaatacc gttatttcag cggctgatgt atgtacaata
     3001 aacggttttt ctttgtcctt actaacttca aacgaagcat taccggataa actaacccga
     3061 cgcatttctt tcccgaaatg tttgtcgtaa gccagcgtag caccgggttg cagggtgatt
     3121 tttgtttcat cgggcaacac agagatagtg gtaaatgcat cagctgtgag cgtttttccg
     3181 ggttcaggtt gcaggaaaat cgttccagca ataatcagaa taagagctgc ggctgccgcc
     3241 ccactccaca cagcaggacg aagtaaaaac cgtttccttt tcccggtaat tttctccaaa
     3301 ctttcatcgg gattgaaaat tcccggtttg taatgcatgg ctacaaaccg gacacttttt
     3361 tcaaggttgt tttcgttatt ggacatatta atattgtttg tctgcccccc aaccccctcc
     3421 cgccttgcgg gataaattgc gggggagtta cagagtgctt attaacgttt attgttcttt
     3481 aattcttaaa tggtaaatgg taaatggtaa aatggtcatt gcattttcct ctaagatgca
     3541 aaaaacaaaa aatccctcac tgctggtgaa agatttttac gcaaaaaaga ataaaaatgt
     3601 gttggggtct ttttgtcgaa gagatttgag tgctcttgtc acgtgatttt caaccgtttt
     3661 aaccgaaagt ccggtttctt cggctatttc cttgtaagcc attccgtcgc gttttgccat
     3721 cagaaaaatt tcacgttgac gttcggggag ttcgtcaatc attttccaga gtttggcttc
     3781 cagttcagct cgtgctatta cttcttccgg tgtatattca tccatcagtt gaagttcttc
     3841 cacactgtca aattcctttt tgctgcggag tttcatcagg cagttgttcc tcacagccgt
     3901 atagagatag gatttgatgg atactatctt atgcagtgta tcccgttttt cccataacgc
     3961 cgcaaaagta tcctgcacca catcttcggc ttcatccgca ttatccaaca gccgcaatgc
     4021 aaacagacaa agtggccggt aatagttatg aaactgggtt gaaaaatatc gttggggatt
     4081 gctcatggaa taccttcatc actaaaaagt gtgacaaaga tactattatt ttaagaatt
//
LOCUS       DAOPZK010000161         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_28316_length_4118_cov_18.362048, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000161 DAOPZK010000000
VERSION     DAOPZK010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28316_length_4118_cov_18.362048"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1086
                     /locus_tag="PKH68_08235"
     CDS             226..1086
                     /locus_tag="PKH68_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNX12965.1"
                     /translation="MKRIAIQGVGGAFHDIAARGYFDNEEVETVPCHTFKDVFKAIDK
                     DDNLLGIIAIENTIAGSLLQNHNLLRESGCLIIGEYKLRISHQLAVLPGQKLEDINEV
                     CSHPIALMQCEDFLDAHKNLRAVETDDTALSAKEIAENKITGRAAICSRLAAELYGLE
                     ILAEGIETNKRNFTRFLIIAKPDLAYKMNRQMVLNKSTLVFTLPHEEGSLSKVLSVLS
                     FYHMNLTKIQSLPIIGREWEYQFYINLTFDDYERYRQSLDAIRPLTNEFQILGEYREG
                     KTPEDPLVAP"
     assembly_gap    1160..1259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1260..2399
                     /locus_tag="PKH68_08240"
     CDS             <1260..2399
                     /locus_tag="PKH68_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNX12966.1"
                     /translation="NRLNSVSEYYFSAKLREIDEMKSQGKNVLNLGIGNPDLPPSEET
                     IEALCTEARKPNVHGYQSYAGIPELRNGFAAWYKRWYNTELNPATEIQPLIGSKEGIL
                     HITLAFVNPGDSVLVPNPGYPTYSSASRICEANVMTYDLDEKNNWEPDFDALEQMDLS
                     TVKLMWVNYPNMPTGANASVELFEKLVAFGRKHNIVICNDNPYSFILNDRKLSILSIE
                     GAKDICIELNSMSKSHNMAGWRIAMLASNEQFVKWILKVKSNVDSGMYRPMMIAAAQA
                     LQNTDEWHNRMNEVYTRRRELAFRIMDTLECTYDRNQVGMFVWGKIADTWQDAGEIAD
                     KILYEKNVFITPGFIFGDKGKRYLRISLCSPERMLEEVIERIQPL"
     gene            2514..3572
                     /locus_tag="PKH68_08245"
     CDS             2514..3572
                     /locus_tag="PKH68_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HNX12967.1"
                     /translation="MELQSILLPGVDAKRPLIISGPCSAETEEQVMATAVALQKIGVK
                     IYRAGVWKPRTKPGGFEGVGTEALTWLKRVKSELGMYTAVEVANAKHVQEALANDVDI
                     LWIGARTTANPFSVQEIADSLVGTDIPVLIKNPVNPDLDLWIGAIERIYNAGIHRIGA
                     IHRGFSSSDKKVYRNLPQWHIPIELHRQLPNLPIICDPSHIGGKRELIASISQQAMDL
                     NFDGLIIESHCTPDKAWSDASQQITPEVLQLILDTLVIRETNQTTESLTVLRRQIDIL
                     DNDLLELLAKRMRVSKEIGTYKKEHNMPILQAQRYDEILKNRIGQAEKMGMDGEFMKT
                     VLMAIHEESIRQQMLIMK"
     gene            3676..>4118
                     /locus_tag="PKH68_08250"
     CDS             3676..>4118
                     /locus_tag="PKH68_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNX12968.1"
                     /translation="MEHKKNLTSEQELELIRVLKNRFEKDLHRHPLLEWADIEQKLAS
                     NAEKLRSLSEMEASGGEPDAVWFDNVTNEYVFMDCSAETPKGRRSLCYDREAWEARKE
                     FKPKTSAVELASEMGIELLSEDEYRELQKLGEFDLKTSSWVKTPA"
BASE COUNT         1268 a          885 c          899 g          966 t
ORIGIN      
        1 aaaatgtatt acttttgtac cgttcaattc aaaatatcta ttattcaata atgaagactt
       61 tatttgcata tttttatttt tacttttact ttagcaggta agggcaggat taaatatgtg
      121 taaattttga aataaatcaa cgaattatac gatatcaaaa aatcctgccc gaagtttcgg
      181 gcaggatttt ttttaatgat ccaattctaa aatacactta acaatatgaa acgaatagct
      241 attcagggag taggcggggc atttcacgat attgcagccc ggggatattt tgacaatgaa
      301 gaagttgaaa ccgtaccctg ccacaccttc aaagatgtat tcaaagccat tgacaaagac
      361 gataatctac tgggtatcat tgctatagaa aataccatcg ccggaagcct gcttcaaaat
      421 cacaacctgc tgcgcgaaag cggttgcctg ataatcggtg aatacaaatt gcggatttca
      481 caccaactgg ctgtgcttcc gggacagaag ctggaagaca tcaacgaggt ttgctcccac
      541 ccgattgcac taatgcaatg tgaagatttt ctggacgccc ataaaaacct gcgggctgtg
      601 gaaactgatg acaccgcact ttcggccaaa gaaattgcag aaaataaaat aacaggaagg
      661 gcagcaatat gcagccggtt agcagccgaa ctctacggcc ttgaaatctt agctgaaggc
      721 attgaaacga ataaacgcaa ttttacccga tttttgatta ttgccaaacc cgatctggct
      781 tataaaatga accgccagat ggtgctgaat aaatcaacac tggttttcac ccttccacac
      841 gaagagggaa gcctgtccaa agttttatcg gtcctttctt tttatcacat gaatctgacc
      901 aaaatccagt ccttacccat catcggacgg gagtgggaat atcaattcta catcaacctg
      961 acctttgacg attatgaacg ctaccgtcag tcgctggacg ctataagacc tcttaccaat
     1021 gagtttcaga ttttgggtga atacagagaa ggaaaaacac cggaagatcc gctagttgcc
     1081 ccctaatccc ctaaagggaa gccccctaaa cccttaaagg taaagccccc taacccccta
     1141 aagggggaat aagaaaactn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1261 accggttgaa cagcgtttcg gaatattatt tttcggcaaa actgcgcgaa attgatgaaa
     1321 tgaagtcaca gggaaaaaat gttctcaatc tgggtatcgg taatcccgat cttccaccat
     1381 cggaagaaac catcgaagct ctctgtaccg aagcccgcaa acccaatgtg cacggctacc
     1441 agagttatgc aggaattccc gaactgagaa acggatttgc agcgtggtac aaacggtggt
     1501 acaacaccga gctcaatcct gcaactgaaa ttcagccgct catcggttcc aaagaaggta
     1561 tccttcacat tacccttgct tttgtcaatc ccggcgacag tgtgctggta cccaatcccg
     1621 gttaccccac atatagcagt gcaagcagga tatgtgaggc aaatgtaatg acttacgacc
     1681 tggatgaaaa aaataactgg gagcccgatt ttgatgcatt ggaacaaatg gatttatcaa
     1741 cagtgaagct gatgtgggtc aattatccca acatgcccac cggagcaaat gccagcgtcg
     1801 aactatttga gaaactggtt gccttcggac gaaaacataa tattgtcatc tgcaacgata
     1861 atccttacag ttttatcctc aacgaccgga agttgagcat cctctctatc gaaggagcaa
     1921 aagatatctg cattgaactc aactccatga gcaagtcgca caatatggcc ggatggcgta
     1981 tagccatgct ggcaagcaac gagcagttcg tgaaatggat attgaaagtg aaatcaaatg
     2041 tggactcggg catgtatcgt cccatgatga ttgcagccgc tcaggcattg cagaataccg
     2101 atgagtggca caacagaatg aacgaagtgt atacccgtcg acgcgaactg gctttccgga
     2161 tcatggacac attggaatgt acctacgaca gaaaccaggt gggcatgttt gtgtggggaa
     2221 aaatagccga cacatggcag gacgccggcg aaatagccga taaaattctg tacgagaaaa
     2281 acgtatttat cacaccggga tttattttcg gtgataaggg gaaacgatac ctgcgcatct
     2341 cgctttgcag ccccgaaagg atgctggaag aggtgataga gagaatacag cccctctaaa
     2401 tctccccgaa ggggagactt ttaaaaccaa ttttcacaaa aacaaaaaat aaactataaa
     2461 aaccttcgca ctcaggtttt ccccctttgg gggaataaaa gggggcttta tttatggaac
     2521 tacaatcaat attattaccg ggcgttgatg ctaagcgtcc gcttataatt tcgggtccct
     2581 gcagtgccga gaccgaagag caagtaatgg caacagccgt tgcattgcaa aaaataggtg
     2641 ttaaaattta cagagcaggc gtatggaaac cgcgtaccaa acccggaggt ttcgaaggtg
     2701 tgggtaccga agcactcacc tggctgaaac gtgtaaaatc ggaactcggc atgtacacag
     2761 ccgtggaagt agccaatgca aaacatgtac aggaagcact tgctaacgat gtggacattc
     2821 tgtggatagg tgctcgaacc acagctaacc cattttcggt acaggaaata gccgattcac
     2881 tcgtgggaac cgacatccct gtgctgatta aaaacccggt aaaccccgat ctggatcttt
     2941 ggataggtgc catcgaacgg atttacaatg ccggtattca ccgaattgga gctattcacc
     3001 gcggctttag ctcatccgac aagaaagttt atcgcaactt accccaatgg catatcccaa
     3061 ttgagttgca ccgtcagtta cccaatctgc caattatttg cgatccatca cacattggag
     3121 gcaagcggga actgattgca tctatcagcc aacaagccat ggatttgaat ttcgacggtc
     3181 tgattattga atcgcactgc actcccgata aagcctggag cgatgcttcg cagcaaataa
     3241 cacctgaagt attgcaactt atactggata cactggtaat acgcgagacc aatcaaacca
     3301 ctgaaagtct caccgtattg cgtcgccaga tagatattct cgacaatgat ttactcgaac
     3361 tgctggcaaa gcgtatgcgg gtatcgaaag aaatcggaac gtataaaaaa gaacataata
     3421 tgcctatcct gcaggcgcag cgctacgacg agatattgaa aaaccgtata gggcaggccg
     3481 aaaaaatggg catggatgga gaatttatga aaacagtact gatggccatc catgaagagt
     3541 caatcagaca acaaatgctc ataatgaaat aatacagctg acaatttata atttttaata
     3601 aggatgggaa tttacctgtc cttatttttt ttacatttgc gggaaactgt ttttgcaaat
     3661 aattttacta cagccatgga acataaaaag aacctgacat cggaacaaga actggaactt
     3721 atccgggtgt taaagaatcg ttttgaaaaa gaccttcacc ggcatccact tctggaatgg
     3781 gcagatattg agcaaaaact ggcatcgaat gctgaaaaac tccggtcgct cagtgaaatg
     3841 gaagcatcag gcggtgagcc ggatgcagtt tggtttgaca atgtcactaa cgagtacgtc
     3901 tttatggact gctcagccga aactcccaag ggtcggagaa gcctgtgcta tgaccgtgaa
     3961 gcatgggaag cgagaaaaga attcaagcca aaaaccagtg ccgtggaact ggcatcagaa
     4021 atgggtattg aactcctgtc ggaagatgaa tatcgtgagt tacaaaagtt gggtgagttt
     4081 gacctcaaaa cttccagttg ggtgaaaact ccggctga
//
LOCUS       DAOPZK010000162         4032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_29224_length_4032_cov_21.967563, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000162 DAOPZK010000000
VERSION     DAOPZK010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29224_length_4032_cov_21.967563"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..854
                     /locus_tag="PKH68_08255"
     CDS             99..854
                     /locus_tag="PKH68_08255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX12969.1"
                     /translation="MKVSIITVTYNSEKSLQRSIDSVISQDYLDIEHIIIDGGSADNT
                     IGIINENKKHIAKYISEKDSGIYQALNKGIRLATGDIIGILNSDDVFADTNIISKVVD
                     SFSKNNSDVVYGNLLYVAKNQSENKTVRYWKSNPYNPKFLIFGWMPPHPTLFCKKEIF
                     QTYGLYNESLQISSDYDFILRIFKETKLKKSYIPEVMVKMTAGGKSNGSFRNICRKTH
                     EDLRAIRANKVGGIYTLFFKNIRKLKQLIPLYH"
     gene            862..1668
                     /locus_tag="PKH68_08260"
     CDS             862..1668
                     /locus_tag="PKH68_08260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX12970.1"
                     /translation="MLNASLVLFNHSVTETAPLVNALRQSACVKDVFLIDNSPVQNPD
                     FRKLPATYIFNNKNLGYGAGHNIAIRKSMEQNVPYHLVVNPDIQFESSILDELVAFKQ
                     KNPDVGLLMPKVLYPSGEIQYLCKLLPKPSDLFIRRFLPPKWTQQSNEQFELRQSGYD
                     KIMDVPYLSGCFMLLRTGALKSVGLFDERFFMYPEDIDLTRRIHREYRTVFYPNVSVT
                     HHHTQGSYKTGRLLWIHLTNMIRYFNKWGWFFDTERKKVNEKILEQLKML"
     gene            1705..2280
                     /locus_tag="PKH68_08265"
     CDS             1705..2280
                     /locus_tag="PKH68_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNX12971.1"
                     /translation="MNFDLQQIISAFIAVFVIIDILGSIPIIMGIKEKNDDIKPLRVC
                     LISFAIFILFLYGGEGILRLFKVDFQSFAVAGSLIIFVYAVEMVLDIEIFKNKGPEGS
                     TSIVPLAFPLVAGPASFTTILSIRAEYAIENILIAIIMNLIFVYIVLRMTEKIEKLIG
                     KGGIYIMRKFFGIILLAIAMKLFMGNIGSLL"
     gene            complement(2374..>4032)
                     /locus_tag="PKH68_08270"
     CDS             complement(2374..>4032)
                     /locus_tag="PKH68_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNX12972.1"
                     /translation="AACVKHFALNNQEEWRGHINVNLSDRALYEIYLPAFKAAVTEAK
                     VWSIMGAYNQIRGQHCCHNDLLLNKILKTDWKFDGVVVSDWGGVHDTRQAALYGLDVE
                     MGTYTDGLTSKAKFDYKDYYLAQPFLAQIKKGEIPEAVLNDKVSRILRLIFRTNMNRN
                     RPYGKFVSPEHSQTALRIAEEGIVLLKNENNILPVASEKYTSIAVIGENAVKTLTLGG
                     GSSSLKVKYEVSPLEGLVKIYGKDKIHYSMGYSSGPSAYDKVVPPVENQDSLLKSAVE
                     IASKADVVLFFGGLNKNQYQDCEGGDRKSYDLPFGQNRLISEILKVNKNLVIVLTTGN
                     AVAMPWIKEVPAIIQNWYNGSENGNAIARVISGAVNPSGKLPFSFPVKLEDNAAHSFG
                     KLSYPGDSINQYYKEDILVGYRWFDTRKIKPLFPFGYGLSYTSFGYGKIATDKKEYAK
                     DETVNVTFTLKNTGTTDGYETVQIYASQPKASVIRPVKELKGFKKVFLQPGETATVTI
                     PIRIQDLAFYDERTGNWNVEAGEFVFHNASSAADVKSSVKIRVK"
BASE COUNT         1142 a          864 c          806 g         1220 t
ORIGIN      
        1 aacagtttac atgggacaaa acctatgaac agcataaaca tttgtatcgt actcttaagc
       61 ataataagtt tgattaattt ttcaagactt ctttttacat gaaagttagc attattacag
      121 taacatacaa cagcgaaaaa tcactacaac gatctataga ttctgtcatt agtcaggact
      181 atcttgacat tgaacacatt ataatcgatg gtggctcagc tgacaacact atcggtatta
      241 tcaatgaaaa caagaaacat atcgcaaaat atatttcgga aaaagacagc ggtatttatc
      301 aggctctaaa taaaggcatc aggctggcta caggcgatat aattggaatt cttaactccg
      361 acgatgtatt tgcagataca aacattattt cgaaggttgt tgacagtttc agcaaaaata
      421 acagcgatgt ggtttacgga aacttgcttt atgttgccaa aaatcagtcg gaaaataaga
      481 cagttcgtta ctggaaaagc aatccctata atccaaaatt ccttattttt ggctggatgc
      541 cgcctcatcc caccttgttt tgtaaaaagg aaatttttca aacgtatggt ctgtataatg
      601 aatcgcttca gatatcttcg gattacgact tcatccttcg catcttcaaa gaaacaaagt
      661 tgaaaaaaag ctacattcct gaagtcatgg taaaaatgac cgccggaggt aaaagtaacg
      721 gatcttttcg gaatatctgc agaaaaacac atgaagatct gagagcaatc agagcaaata
      781 aagttggcgg tatttataca ctgtttttca aaaatattcg taaacttaaa caactaatac
      841 cactttacca ttaacttaca catgctcaac gcctccctcg tccttttcaa ccattccgta
      901 accgaaacag cgcctttggt caatgcactc cggcaatcag cgtgtgtgaa ggatgttttt
      961 ctgattgata attctcctgt tcaaaatccc gatttccgga aacttccagc aacatacatt
     1021 ttcaacaaca aaaacctggg gtatggagcc ggccataata tcgctatccg taaaagtatg
     1081 gagcaaaatg ttccctatca tctggtagtc aacccggata tacagttcga aagtagcatt
     1141 ttggatgaat tggtagcttt taagcagaaa aaccccgatg tgggtctgct gatgcctaag
     1201 gtgttgtatc catccggtga aattcaatat ctctgcaaac tgctgccaaa accatctgat
     1261 ttattcatcc ggcgctttct cccaccgaag tggacacaac aatcaaacga gcaattcgaa
     1321 cttcgtcagt ccggttatga taaaataatg gatgtgccct atttatccgg ctgttttatg
     1381 ctgttgagaa ccggtgcgtt gaaatcggtc ggcttgtttg atgaacggtt tttcatgtac
     1441 cccgaagata ttgaccttac cagacgcatt caccgtgaat accggactgt tttttatccc
     1501 aacgtcagtg tcacgcacca tcatacacag ggatcataca aaacggggcg cctgctgtgg
     1561 attcacctca ccaatatgat ccggtatttc aataaatggg gctggttttt tgataccgaa
     1621 cgaaaaaaag tgaatgaaaa aatcttggaa caactgaaaa tgttgtaatt ttgcaaaata
     1681 atacgaatta aatacgtcgt tagtatgaat tttgaccttc agcagataat cagtgctttt
     1741 atagctgttt ttgttatcat agacatattg ggttccatac ctattatcat gggtatcaag
     1801 gaaaaaaacg atgatatcaa accactacgt gtgtgtttga tttcatttgc cattttcatt
     1861 cttttcctgt acggtggtga gggaatttta agattattca aggttgattt tcaatccttt
     1921 gcagtggcag gttctcttat aatttttgtt tatgcagtag aaatggtgct ggatattgag
     1981 attttcaaaa acaaggggcc ggaggggagt acgtccattg ttccactcgc atttccgctg
     2041 gtagccggtc cggcatcatt caccaccatt ctttcaatcc gtgccgaata tgcgatcgag
     2101 aatatcctca tcgccatcat tatgaatctg atatttgttt atattgtgct ccggatgact
     2161 gaaaagattg aaaaattaat aggtaaaggc ggaatttata ttatgcggaa gtttttcggc
     2221 atcatcctgc tggccattgc gatgaaatta tttatgggaa atatcggttc attattgtaa
     2281 aaataccaac ataaaataaa ccggccccga ataccatcac tgacattcgg ggccgatcgt
     2341 tttaatcaat ctaattcact caaaatctga ttgttatttt actctaatct ttacactgct
     2401 tttgacatcg gcggctgaag aggcattgtg aaagacaaat tcaccagctt ccacattcca
     2461 gtttcctgtt ctttcatcgt agaaagcaag gtcctgaatt cgtatcggaa tagttaccgt
     2521 tgccgtttcg ccgggttgca gaaatacctt tttaaatcct ttcagttctt ttaccggtcg
     2581 aataactgat gctttcggtt gagaagcata aatttgcacg gtctcatagc catccgtggt
     2641 tccggtattt ttcagggtga aagtcacatt aactgtttcg tcctttgcat attccttttt
     2701 atcggttgcg atcttgccgt aaccaaaact ggtgtacgaa agtccatacc caaatgggaa
     2761 gagtggtttg atttttcggg tatcgaacca tcggtaaccc accagaatat cttccttgta
     2821 atactgattg atgctgtcgc cgggatagga gagttttcca aaagagtgtg ccgcattgtc
     2881 ttcgagcttc accgggaaag agaatggcag cttacccgat ggatttacag cgcccgaaat
     2941 gacccgtgca atggcattac cgttttcgga gccgttgtac cagttttgta ttatagcggg
     3001 aacttctttt atccatggca ttgctaccgc attgccggta gtaagtacga tgaccaggtt
     3061 tttattcact ttcagaattt cgctgatgag gcggttctga ccaaacggta agtcatacga
     3121 tttgcggtcg ccaccctcac agtcctgata ctgatttttg ttcaatcctc cgaagaagag
     3181 taccacatcg gctttggatg caatttccac agctgacttc aacaatgaat cctgattttc
     3241 gacgggtgga acaactttat cgtaagccga cggtccggat gaataaccca tggaataatg
     3301 gattttatct ttcccgtaga ttttcacgag cccttccagt gggcttactt catattttac
     3361 cttcaacgaa gaggagccgc ctccaagggt aagcgttttt actgcatttt cgccgataac
     3421 agcgattgaa gtatattttt cggaagcaac tggaaggata ttattttcat ttttaagcaa
     3481 cacaattcct tcttcggcta tccggagtgc agtctgactg tgttcgggcg aaacaaactt
     3541 tccgtaggga cggttacggt tcatattcgt acggaaaatc agccgaagaa tgcggctcac
     3601 tttatcgtta agtaccgcct ccgggatttc tccctttttt atttgtgcca gaaatggttg
     3661 tgccagataa taatccttgt agtcaaattt tgctttcgaa gtcaatccgt cggtgtaggt
     3721 tcccatttct acatccaaac cgtagagagc agcctgacgg gtatcatgca ctccgcccca
     3781 gtcgctcacc actaccccat cgaacttcca gtcagttttc agtattttat tcagcaacaa
     3841 gtcattatga cagcaatgct gcccgcgtat ctgattatat gcgcccataa tcgaccacac
     3901 ttttgcttcg gtgactgccg ctttgaatgc cgggagatag atttcgtaca aggcccggtc
     3961 actgaggttc acattgatat gcccgcgcca ttcttcctga ttgttcagcg caaaatgctt
     4021 gacacatgcc gc
//
LOCUS       DAOPZK010000163         4028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_29275_length_4028_cov_17.507677, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000163 DAOPZK010000000
VERSION     DAOPZK010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29275_length_4028_cov_17.507677"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            602..2797
                     /locus_tag="PKH68_08275"
     CDS             602..2797
                     /locus_tag="PKH68_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNX12973.1"
                     /translation="MSSLRFKAVEEAFKKKALCVTAPSKFTTDYYGKYVFTKSIMAKY
                     LSKDIMKALNNAIEKGVTLDRSIADDVATGMKMWAMELGATHYTHWFQPLTDGTAEKH
                     DSFIDYAAGPGESIIEDFSGKLLAQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAFV
                     VDDTLCIPTIFISYTGESLDLKAPLLKSLNAVNKAATAVCQLFDPNVKKVITYLGWEQ
                     EYFLIDEGLYATRPDLMLTGRTLIGHESAKNQQMEDHYFGAVPTRVAAYMKDLEFEAY
                     KFGIPLKTRHNEVAPNQFELAPIYGECNLSVDQNLLVMSIMKKVARRHSFRLLLHEKP
                     FAGINGSGKHNNWSLGTDTGVGLLTPGKTPAENLQFITFLVNILMAVYKNNALLKASI
                     MTAQNAHRLGANEAPPAIISAFLGKQITAVLDKLENSTSDEAIVMSDKQQLSLGIAHI
                     PEVLLDNTDRNRTSPFAFTGNRFEFRAVGSSANCSSAMATLNAAVAAQLTEFKLAVDA
                     KIAAGSSEQSAIFEILKEYIKISKPIRFDGNGYSEEWKVEAAKRGLDCETSVPEIYKV
                     FTSEQSVKMFTSTGVLTEKELHARNEVKWETYTKWIQIEARVLGDLSMNHIIPLATKY
                     QSFLLDNVCKIKTVFSGEKADKLSTHDTALIVEIGDRVAAIKNAVDDMIDIRRTVNKI
                     EDEYEKAVAYHDKVLPYFDIIRYNTDKLELIVDDEMWTLPKYREMLFIR"
     gene            2954..>4028
                     /locus_tag="PKH68_08280"
     CDS             2954..>4028
                     /locus_tag="PKH68_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HNX12974.1"
                     /translation="MSLSPLQIDEYEQRSLYSFQNEHDACGVGLVINLSGDKSHEIVD
                     NGLQVLENMVHRGAESADNKTGDGAGILVHIPHEFILLQGIAVPSKGKYGTGLVFLPK
                     DKKKSNDCLSVLKDYVEKEGLTLLAVRDVPVNSQILGEISASNEPDIKQIFVTGNLPQ
                     DELETKLYIVRKKIEKAIQQSKLAAERSFYIVSLSTKQMIYKGMLTSLQLREYYPDLS
                     NPNFTSRIALVHSRFSTNTFPTWDLAQPFRMIGHNGEINTIRGNRQWMESRESVLKSD
                     VLGTNMQDLYPIVQPGMSDSASLDNVLEFLVMSGKSLPHALAMLVPESFNDKNPISDG
                     LKAFYEYHSMLMEPWDGPATLLFS"
BASE COUNT         1244 a          885 c          843 g         1056 t
ORIGIN      
        1 acgagacaca acacggagaa actgcgtata ccagtttcga ataaatataa aaaaagtgaa
       61 ctaatgattg gctcagtggc gtcaatcaga gaataaaaca gattgaaaag aggtttgcca
      121 tcgggtgaaa tacttcaggt tgaacccggg taataattac agaaattacg ataaaattga
      181 tttgctttca gtttatcgta tgttttgagt tttattgttt cgggcagact acttcgtgat
      241 gaaatagtct gcccttttta aaacacattc tgtaagctat atccaatttt tattgttatt
      301 tttatttctt ttatcgattt tacaagtcat tttgtcatta taattaaatt tttacagaaa
      361 ttttttaaac aacaaacaaa caacaaaatt tactgtttat aacttttctg tgattttttg
      421 tatcattcta tcatttttta ttttgtttta gttacacttt attactcaac atataacagg
      481 atttaaaaat accacattct attgggatta gcttacaaaa tgtctaattt tgcatcaact
      541 aaaaagaagt tttatagaaa ttgatttgaa tttaaaacaa acaatataat tacatacaat
      601 tatgtcatca ttaagattta aggcagtaga agaagcattt aagaaaaaag cgttgtgcgt
      661 tacagcacct tcgaaattta caaccgatta ttacggtaaa tatgtattca ccaaatccat
      721 tatggccaaa tatttgtcca aggatataat gaaagcgttg aataatgcca ttgaaaaagg
      781 cgttaccctc gatcgaagca ttgccgatga cgtagccacc ggtatgaaaa tgtgggctat
      841 ggaactggga gcaacgcact atacacactg gtttcagcca cttaccgacg gaactgccga
      901 aaagcacgac tcgtttatcg attatgcagc cggacccgga gaatctatta ttgaggattt
      961 ctcgggaaaa ctccttgcac agcaggaacc cgatgcgtca tcgttcccca gcgggggcat
     1021 ccgcagcaca ttcgaagcgc gcggctacac cgcctgggac ccatcttcac cggcttttgt
     1081 tgtggacgac accctttgta ttcctaccat attcatctct tataccggag aatcgcttga
     1141 cctgaaagct cctttgctca aatcacttaa cgctgtcaac aaagcagcaa cagcggtttg
     1201 tcaactattt gacccgaatg tgaaaaaggt aattacctat ctcggttggg aacaggaata
     1261 ttttctgatt gacgaaggat tgtatgcaac ccgccccgac ctgatgctta caggtcgtac
     1321 tcttattggt catgagtcgg ctaaaaatca gcaaatggaa gaccattatt tcggtgcagt
     1381 acctacccgc gttgccgctt acatgaaaga tctggaattt gaagcttata aattcggcat
     1441 tccactgaaa acccgccaca atgaagtagc tcccaatcag tttgagctgg ctccaatcta
     1501 tggtgaatgt aatctttcgg tggatcagaa cctgttggtg atgtccataa tgaaaaaagt
     1561 ggcccgccga cacagtttcc ggctgctgtt gcacgaaaag ccttttgcag gcatcaacgg
     1621 atctggtaaa cacaacaact ggtcgctcgg aacagatacc ggcgtgggat tgctcacacc
     1681 gggtaaaact ccggcagaaa accttcagtt catcaccttc ctggtcaaca ttctgatggc
     1741 tgtttataaa aacaatgcat tattgaaagc atcaatcatg acagctcaga atgcacaccg
     1801 gttgggtgcc aacgaagcac ctcctgccat catttcggca tttcttggga aacagattac
     1861 ggctgtgctt gataaactgg aaaacagcac cagtgatgaa gccattgtga tgagtgacaa
     1921 acaacaacta agtttgggta tcgctcacat ccccgaagta ttactcgaca ataccgaccg
     1981 caaccgaact tcaccgtttg cttttaccgg aaacaggttc gaattccgtg ccgtaggctc
     2041 ctcagccaac tgttcctctg caatggcaac gctgaatgca gccgtagcag ctcagctgac
     2101 tgaatttaag ctggctgtag atgctaaaat cgctgccggt tcatctgagc agagtgccat
     2161 ttttgaaatt ctgaaagaat atatcaaaat ctcaaaaccc atccgttttg acggtaacgg
     2221 atacagtgaa gaatggaaag tcgaagctgc caaacgcggc ctggactgcg aaacaagtgt
     2281 tccggaaatt tacaaggtat tcaccagcga acaaagcgtg aaaatgttca catccacagg
     2341 cgtactcacc gaaaaagaac tccacgcccg caacgaagtg aaatgggaaa cgtacaccaa
     2401 gtggatacag attgaagcgc gggtgttggg agatttatcc atgaaccata tcattccgct
     2461 ggccacaaag tatcagagct tcctgctcga taatgtatgt aaaataaaaa cggttttcag
     2521 cggcgaaaaa gcggataaac tcagcacaca tgacacagca ctaattgttg aaataggcga
     2581 ccgcgtagca gctatcaaaa atgcagtgga cgatatgatt gacatccgcc ggacggttaa
     2641 caagatagaa gatgaatatg aaaaagcagt ggcttatcac gataaagtgt tgccctattt
     2701 cgacattatc cgctacaaca ccgacaaact ggaactgatt gtggatgatg aaatgtggac
     2761 attgcctaaa taccgggaaa tgctgtttat cagataaaca attgattttc aaattctttc
     2821 tcatcataaa aaaaaatagt gagagtttgt aatcaacaaa acttctcact attttttaga
     2881 ataaaactta aaacaaaacc gatagcaagc gagatcgttt ctccggaagc aacctcccaa
     2941 ttaccaaccc attatgagtt taagtccatt acaaatagat gaatatgagc agcgttcgct
     3001 gtattcattc caaaacgagc acgatgcctg cggagtaggc ctggttatta atcttagtgg
     3061 cgacaaatcg cacgaaattg tggataatgg tctgcaagtg ctcgaaaaca tggtgcaccg
     3121 cggagccgaa agtgccgata ataaaacagg tgacggagcc ggaattttag tgcacattcc
     3181 gcacgaattt attcttttac agggcattgc cgttccttcc aaagggaaat acggtaccgg
     3241 attggttttt ctgcccaagg ataaaaaaaa atcgaacgac tgtctgagtg tactcaaaga
     3301 ttatgtcgaa aaagagggat tgacactgct tgcagtacgt gatgtgccag tcaacagtca
     3361 aattctgggt gaaatttctg cttctaacga accggatatt aagcagattt tcgttaccgg
     3421 taacctgcca caggacgagc tggaaacaaa actttacatt gtccgaaaaa aaatagaaaa
     3481 agctattcag cagtcgaaac ttgccgctga acgtagcttc tatatcgtta gcctttccac
     3541 taaacaaatg atttacaagg gaatgctcac ctcacttcaa cttcgcgaat actatcccga
     3601 cctgagcaat ccgaatttca ccagccgcat cgccctggtt cattcacgtt tcagcaccaa
     3661 cacattcccg acctgggatt tggcgcagcc tttccgcatg atcggccaca acggtgaaat
     3721 caacaccatc cggggtaacc gccagtggat ggaaagtcgt gaaagtgtgc tcaaatcgga
     3781 cgtgctgggt acgaatatgc aggacctcta ccccatcgtg cagcccggca tgagcgacag
     3841 cgcttcgctg gataatgtgc tggaatttct ggtgatgtcg ggcaagagcc tgccacacgc
     3901 gctggcaatg cttgttccgg aaagtttcaa cgataaaaat cctatttcgg atggcttgaa
     3961 ggcattttac gagtaccaca gcatgctgat ggaaccctgg gacggacccg ctacactgct
     4021 tttcagcg
//
LOCUS       DAOPZK010000164         4020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_29354_length_4020_cov_19.516520, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000164 DAOPZK010000000
VERSION     DAOPZK010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29354_length_4020_cov_19.516520"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKH68_08285"
     CDS             <1..214
                     /locus_tag="PKH68_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12975.1"
                     /translation="KLIAHKFKYNYSENIKKWCKVYKVHQVRQVLKFIKFPETSSGQV
                     SDLSSFPDDVKLSCLSVCFMLNYAGC"
     gene            242..565
                     /locus_tag="PKH68_08290"
     CDS             242..565
                     /locus_tag="PKH68_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12976.1"
                     /translation="MKRLKVGILLGIIAGIIDVIPMIMQNLTWDANISAFTMWIVVGF
                     LIATIDLKINSIIKGILTAFLVLLPTAILIGWKEPHSLIPIVLMTTILGGLLGFSINK
                     ILDKK"
     gene            749..1042
                     /locus_tag="PKH68_08295"
     CDS             749..1042
                     /locus_tag="PKH68_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12977.1"
                     /translation="MKENLMPYKNSISGKLVFFLTIIVAYYWFLGQVTDVYRFEVVGA
                     VYEILWLPVLVMLFVLPVIALVLLIKEKRNIWSLNIYSLLIGVVTVLCMIFGK"
     gene            1177..1398
                     /locus_tag="PKH68_08300"
     CDS             1177..1398
                     /locus_tag="PKH68_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12978.1"
                     /translation="MKFPWFKRIGILFIPRSIIGWILLAAAIVYAVSVFIDIDSRSHS
                     VSDTLMNFVFNLVIISVVYSLIAYLTSRK"
     gene            1662..2258
                     /locus_tag="PKH68_08305"
     CDS             1662..2258
                     /locus_tag="PKH68_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX12979.1"
                     /translation="MKRNLYRTIVGSVFFVFCILLPVAVTAQFHVGLQSGLVFSKAGM
                     ITKSNDFGYLNYAYPTPTLNYIMTNATNLHVSYTLSPSFTLAVEPGYMQKSNEQNGGL
                     TYIQLPVLAEYTLIDRLRLTAGPELNFLLNNDSEKSYKGLDVAVQLGAYYGVYSTFDL
                     GFKFSRSTGPFLKHDIVSSGSDINFYHYYFQFFTRYKF"
     gene            2291..3733
                     /locus_tag="PKH68_08310"
     CDS             2291..3733
                     /locus_tag="PKH68_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNX12980.1"
                     /translation="MKTLEPTIVWNHFYEITQIPRPSKKEDKVRAYLDEFGKKHNLTT
                     VTDKAGNVLITKPATPGYENEKTIILQAHMDMVCEKNSDVEHNFDTDPIQVIVDNGWI
                     KAKGTTLGADNGIGMALMLAVLSSDNLKHPALECLFTTDEETGLTGAFALDNNLLKGE
                     ILINLDSEDDGEIFVGCAGGIDTTATLKVKTKDTPRHFFAFSVTVKGLMGGHSGDDID
                     KRRGNANKMLNRILWNMHKDLDLHLVKIDGGNLRNAIPREASAVACVPFVHKEKVRVI
                     FNEVAAELEDENAAIETNMRIELHSEEIPDRVMTRRSTSKILNALYACPHGIKAMSPD
                     MAGLVETSTNLASVKMPDDKKLVITTSQRSSVESAKYDIAHQVESVLKLAGCSVTHGD
                     GYPGWKPNLNSQILRVAEKSYEKLFAVQPKVKAIHAGLECGLFLKKYPHLDMISIGPT
                     MRGVHSPDERLEIETVGRVWSWLTDILATK"
BASE COUNT         1233 a          774 c          814 g         1199 t
ORIGIN      
        1 gaaacttata gctcacaaat ttaaatataa ttattcagaa aatatcaaga aatggtgtaa
       61 agtttataaa gttcatcaag ttcgtcaagt tttgaagttc atcaagtttc ccgaaacaag
      121 ttcgggacaa gtatcagatt tatcaagttt ccccgatgat gttaaacttt cctgtttgtc
      181 agtgtgtttt atgctaaact atgctggatg ttaaaaagta agattactta acttatatga
      241 catgaaaaga ttaaaagttg ggattttatt aggtattatt gccggtatta ttgacgtaat
      301 tccaatgatt atgcaaaatc taacgtggga cgctaatatt tctgcattta ctatgtggat
      361 agttgtcgga tttttaattg cgacaattga tttaaaaatc aattcgataa tcaaagggat
      421 tttaactgct tttttggttt tattgccaac tgccattttg atagggtgga aagaacctca
      481 ttctttgata ccaattgttt taatgacgac aatactgggt ggattactgg ggttttcaat
      541 caataaaatc ctggataaaa aatgatttcc tttaaatatg atgaataaag cacaatcata
      601 taactaaaac tatgtgttaa ttgggacttc cgtgtggaca aaaaagttcg aaatatgcaa
      661 taactattgt aatgctctat aggaaaaaag cccgcaattc ccaattgcat atatttccga
      721 tggactattt actaataaat attgaaagat gaaagagaat ttaatgccat ataaaaattc
      781 tatttccggt aaactggttt ttttcctgac cattattgta gcctactatt ggtttctcgg
      841 acaggtcact gacgtatatc gttttgaggt agttggtgct gtctatgaga ttttgtggct
      901 tccggtgctg gtgatgttgt ttgtactgcc tgtaattgcg ctggttttac tgattaagga
      961 aaaacggaat atctggtcgc tcaatatcta ttctttactt ataggtgtgg ttactgtttt
     1021 gtgtatgatt tttggaaaat aaaacaacgt acattgtagt gtgaaagtaa aaagctagtc
     1081 ttgatgcaat ttaatatcat taatgcaatt ttgttaaatg ctttgatcaa cttttttcag
     1141 cgaggtattt cttattcact ataaatgctc cacaccatga aatttccctg gttcaaacga
     1201 attgggatat tatttattcc aaggtctatc atcggttgga tactacttgc agcagcaata
     1261 gtttatgccg tatctgtttt catcgatatc gacagccggt cgcattcagt gagcgataca
     1321 ctcatgaatt ttgtatttaa tctggtgatt atctcggtgg tatattcgtt gattgcttat
     1381 ctgactagca gaaaataaag attataagtg aaattcaccc tacccccaat tttttcgggt
     1441 agttaacgtt tggtagtgca taaaacttaa ccagccaaca acggtttatt tgaaaattta
     1501 aacagaagaa acttatctat atttttctta gaaattgaat tgttttccac gaccatcatt
     1561 attcgttcct gtgaaatatt taacacaaat aacaccaaca aaaccttctt tcaattcttt
     1621 tgtttatatt tgtgttctgt aataaatcta aaacaaaaga tatgaaacga aatctttacc
     1681 ggacgattgt tggtagtgta ttctttgttt tctgtatctt attacctgtt gcagttactg
     1741 cacagtttca tgtagggttg caatcgggcc ttgtattctc caaagcggga atgattacca
     1801 aaagtaacga ttttggatat ctcaattatg cttatccaac tccaacgctg aactacatca
     1861 tgacaaatgc caccaatctg catgtgagtt atactctttc gccctctttc acgctggctg
     1921 tggagccggg ttatatgcag aaatccaacg aacaaaacgg tgggttaacc tacattcagc
     1981 tgccggtgtt agccgaatac acgctgattg accgcttgcg gcttactgcc ggcccggaac
     2041 tcaattttct gttgaataat gacagtgaaa aaagctacaa aggattggat gttgccgtac
     2101 agctgggtgc atattacgga gtgtacagca ccttcgatct gggcttcaag ttcagccgct
     2161 ccacaggacc gttcctgaaa catgacattg taagttccgg ctcagatatc aatttttatc
     2221 actattactt tcagtttttc acgcgttaca aattttaatt caacaataaa ataattataa
     2281 acaccaaaat atgaaaacat tagaaccaac cattgtttgg aaccattttt acgaaattac
     2341 ccagataccg cgtccttcta aaaaagaaga taaagtgaga gcatacctgg atgaattcgg
     2401 aaaaaaacat aacctgacta ctgtgaccga caaagccggt aatgtgctga ttaccaaacc
     2461 ggcaactccg ggctatgaaa acgaaaaaac gattattttg caggcacaca tggatatggt
     2521 ctgtgaaaaa aacagcgatg tggaacacaa tttcgacacc gatcctattc aggtgattgt
     2581 ggacaacgga tggataaaag ccaaaggaac cactctgggt gcagacaatg gaataggtat
     2641 ggcgctgatg ctggctgtac tctcatcgga caatctgaag caccctgcgc tggaatgtct
     2701 tttcactacc gacgaagaaa ctggcctgac cggcgctttt gcactcgaca ataatttgct
     2761 gaaaggagaa atacttatca acctggactc cgaagacgac ggtgaaatat tcgtaggatg
     2821 tgccggcgga atagacacca ccgctacgct gaaagttaaa acaaaagaca caccccgtca
     2881 cttttttgct ttttcggtta ctgtcaaagg gctgatgggc ggtcactcgg gcgacgatat
     2941 tgataaacga cgcggcaacg ccaacaaaat gctcaaccgc atcctgtgga atatgcataa
     3001 agacctggat ttacacctgg tcaaaattga cggtggcaac ctgcgtaatg ctattccccg
     3061 tgaggcatcg gcggtggctt gcgtcccgtt cgttcacaag gaaaaagtac gcgttatatt
     3121 caacgaagtg gctgccgaac tggaggatga aaatgccgcc atcgaaacaa atatgcgtat
     3181 tgaacttcat tcggaagaaa ttcccgatcg tgtgatgacc cgccggagta cttccaaaat
     3241 cctgaatgct ctctatgcct gtccgcacgg catcaaagcc atgagccccg atatggccgg
     3301 actggttgaa acatccacca acctggcttc ggtgaaaatg cctgacgaca aaaaactggt
     3361 gattaccacc agtcagcgca gttcggtaga atcggccaaa tatgacattg ctcatcaggt
     3421 ggagtcggtg cttaaactcg ccggttgcag cgttactcac ggcgatggat acccgggatg
     3481 gaaacccaat ctgaactcac agatactccg cgtggctgaa aaaagttatg aaaaactgtt
     3541 cgcagtccaa cccaaagtga aagcaatcca tgccggactg gaatgcggct tattcctgaa
     3601 aaaatatccg cacctggata tgatttccat cggaccgacc atgcgtgggg tgcattcacc
     3661 cgacgagcga ctggaaattg aaaccgtggg gcgtgtgtgg agctggctca ccgatattct
     3721 ggcaactaaa taatgtgata aagataacca gagggcgtca gtactaaacg acgccctttt
     3781 tacaactttg aagtgcatat tatttgttga aatccaaacg acaggtatca aatagctaat
     3841 ttagtaaatg aatttctttg agctttttgt gtctgctttg tgttttttgt gtaataaaaa
     3901 aaaatcagta aaaactgacg aagttatagc tgtatttatg acaatcttat gaaaaaacaa
     3961 aacaagcgaa agataatcaa cgatccggtt ttcggattta tcaatgtacc gaatgatttt
//
LOCUS       DAOPZK010000165         4013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_29428_length_4013_cov_22.155887, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000165 DAOPZK010000000
VERSION     DAOPZK010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29428_length_4013_cov_22.155887"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /gene="tpx"
                     /locus_tag="PKH68_08315"
     CDS             complement(<1..389)
                     /gene="tpx"
                     /locus_tag="PKH68_08315"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNX12981.1"
                     /translation="MATTNFKGNPVKTSGELPVVGSKAPDFTLVKGDLSEVTLSDFAG
                     KKLVLNIFPSIDTGVCAMSVRQFNKWVSEKENTVVLCVSKDLPFAASRFCGAEGLDKV
                     ITASDFRYNTFATDYGVLQADGPLKGL"
     gene            557..1756
                     /locus_tag="PKH68_08320"
     CDS             557..1756
                     /locus_tag="PKH68_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX12982.1"
                     /translation="MNKHFTYILLIAATAALFSCRDRTEYRVGSDFQQYVDGFEQEAA
                     LRNRNFNFESSGLIIEFGDLEEGVAGLCHYQKPIRIEVDRDYWNSLSDQEGTELMREE
                     LLFHELGHGILNRTHTNSVLINDEWKSIMCGGDEIAGRTWNINYRGERRKYYLDELFN
                     ESTPEPAFATEGLTVDTTGFATTYTDEFSNTASTKWKLGATSNGTASIENGMLKYVSN
                     SSVNLIILIAAGIDVQSDFIYECTLQYTGFDNTAKYGLVFGTYTNESATVTSDGASLE
                     YILINNDRKMTIGNRAWFSYFTQITRNQIVPQSRNKIKVVKKDDRMYFFINGEYAYRS
                     EMVNRKTGYNYGFSVPPKSTLLIDDFRLAASGTTASAAKIKSAEIENMEMKVVEAKFP
                     VGEINNR"
     gene            1795..3495
                     /locus_tag="PKH68_08325"
     CDS             1795..3495
                     /locus_tag="PKH68_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNX12983.1"
                     /translation="MDIMPKFVIYQVIPRLFGNYNTTRKYKGTIQENGCGKFSSFTPK
                     AFEEIKALGATHIWYTGIIEHATQTDYTAYGILRDHPGVVKGKAGSPYAIKDYYDVDP
                     DLADDVPNRMAEFEDLIRRTHAAGLKAIIDFVPNHTARQYHSDVKPAGVIDLGEADNK
                     NLAFSPRNNYYYIPDTPFQPSFNVEGYTEFPAKATGNDQFTATPSEFDWYETVKLNYG
                     VNYTEGWQKHVDPIPDTWYKMVDILLYWAAKDVDGFRVDMAEMVPVEFWGWAIPQVKK
                     KYPKIIFIAEVYQPAEYRNFICNGKFDYLYDKVGLYDKLRGIMSRNEPARDITAQWQS
                     LNGIQNQMLNFLENHDEQRIASGFFCGSGVYAQPAMIISATLTQSPVMIYMGQELGEL
                     GMDMEGFSGVDGKTSIFDYWGVASIQAWANGGKFDGAHLHQEQKELRAFYQQLLNLCM
                     SEKAITDGVMYDLVFANFGNPNFNTHEQFAYFRKYKKELLLCVVNFQDKNIGMEINIP
                     TEAFQFLDLDENEFYSSVNLLNREEDFPIQRLSSLNPYRVFLPAWSGKILKLKHEKIV
                     "
     gene            3544..>4013
                     /gene="eno"
                     /locus_tag="PKH68_08330"
     CDS             3544..>4013
                     /gene="eno"
                     /locus_tag="PKH68_08330"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNX12984.1"
                     /translation="MSKIEKIVAREVLDSRGNPTVEVDVTLESGVVGRASVPSGASTG
                     ENEAIELRDGDKQRFLGKGVLKAVKNVNEVIAPALIGECALQQRKIDKAMIALDGTKT
                     KSNLGANAILGVSLAVAKAAAIYLDMPLYRYIGGTNAFTLPVPMMNIINGGSHS"
BASE COUNT         1232 a          903 c          876 g         1002 t
ORIGIN      
        1 catagtcctt tgagcggtcc atcagcctgc aatactccgt agtcggttgc aaaggtgttg
       61 taacgaaaat cagatgctgt tatcactttg tcaaggcctt cagctccaca gaaacgcgac
      121 gcggcaaagg gtagatcttt tgatacgcaa agtaccacag tgttttcttt ttcagaaacc
      181 catttgttga actgacgaac cgacatggcg catacgccgg tatctatgct cgggaagata
      241 ttcagtacca gtttttttcc tgcgaaatca ctcagcgtaa cttcacttaa atcgccttta
      301 accagtgtaa aatcaggggc tttggagcct actacaggca gttctccgct tgttttcacc
      361 gggttgcctt tgaaatttgt tgttgccatt tttgatatat taatttttag taaaaataag
      421 attttgttaa tatggaatat aacaaggtga ggcgaatttt gtttatcttt gcagtaaaga
      481 atttaactta aattatcccg aaatatgtct gtcgcatctc cgatatctgt atttcaggta
      541 tttaaattca gccgttgtga ataaacactt cacttatatt ctcctcattg ctgccacagc
      601 ggcacttttt tcgtgcaggg accgtaccga ataccgggtc ggcagtgatt tccaacaata
      661 cgtggatgga tttgagcagg aagccgccct tcgtaaccgc aatttcaact tcgaaagcag
      721 cgggttgatc atcgaattcg gtgatcttga agagggagta gccggcctgt gtcattacca
      781 aaaacccatc cgcatagaag ttgacagaga ttactggaac tcgctgagcg accaggaggg
      841 caccgaactg atgcgcgaag aactgttgtt tcacgaactg ggacacggaa ttctgaacag
      901 aacccataca aattctgttt taataaatga cgaatggaaa tcaattatgt gtggaggcga
      961 tgaaattgcg ggtagaacgt ggaatatcaa ttatcgcggc gaacgcagga aatactacct
     1021 cgacgaatta ttcaacgaaa gcacacccga acccgccttt gcaaccgagg gactgaccgt
     1081 ggataccacc ggttttgcaa ccacttacac cgacgaattc agcaatacag cttccaccaa
     1141 atggaaactg ggtgctacga gcaatggcac agcctccatt gaaaacggaa tgctgaaata
     1201 tgtgtccaac agttcggtta atctgattat tctcattgct gccggtatcg atgtacagtc
     1261 agactttata tatgaatgta ctctacagta cacaggtttt gacaatacag ctaaatacgg
     1321 actggtattt ggcacttaca ccaacgagag cgcaacagta acctctgatg gagcatcgct
     1381 ggagtacata ctcatcaaca atgaccggaa aatgactatc ggcaaccgtg cctggttcag
     1441 ttattttaca cagataaccc gcaatcagat agttccacag agccgcaaca agattaaagt
     1501 ggtgaaaaaa gacgaccgta tgtatttctt cattaacggg gaatatgcct accggtccga
     1561 aatggtaaac cgtaaaaccg gatataatta cggattttcg gtgccaccaa aaagtacctt
     1621 gttgatagac gacttcagat tagcggcaag tggaaccact gcctccgcag caaaaataaa
     1681 gtcagctgaa attgaaaata tggagatgaa agtggtggaa gcgaaattcc ctgtcggtga
     1741 aattaataac agataagaag cagcaaaaag ataattaact caggccttaa ataaattgac
     1801 atcatgccaa aatttgtgat ctatcaggta attccccgat tattcggcaa ttacaacaca
     1861 acccgaaaat acaaaggaac aatacaggaa aacggttgtg gaaagttcag cagtttcacg
     1921 cccaaagcat tcgaagaaat taaagcactg ggagccacac atatctggta cacaggcatt
     1981 attgaacatg ccactcagac cgattacacc gcatatggta tcctcaggga tcatcccggt
     2041 gtggtgaaag gaaaagccgg atcgccttat gccatcaagg attattacga tgttgatccc
     2101 gatctggccg acgatgtacc caaccggatg gccgaatttg aagacctcat ccggcgcaca
     2161 cacgctgccg gcctcaaggc tatcatcgat tttgtaccca atcatactgc ccgacaatat
     2221 cactccgatg tcaaacctgc cggggttatc gatctgggcg aagcagacaa caaaaatctg
     2281 gctttttcgc cccgaaacaa ctattactac atccccgata caccttttca gccatcattc
     2341 aatgtagaag gctacaccga atttccggcc aaagctaccg gtaacgatca gtttacggcc
     2401 acaccttcgg aattcgactg gtacgaaacc gtaaaactga attacggagt aaattataca
     2461 gaaggatggc aaaaacatgt tgatcccata cccgatacct ggtataaaat ggttgacata
     2521 ctcctctact gggcagcaaa ggatgtagat ggattccggg tggatatggc cgaaatggta
     2581 cccgtggaat tctggggatg ggcaatacca caggtcaaaa aaaaatatcc caaaataata
     2641 tttattgcag aagtgtacca accggccgaa taccgcaatt ttatctgtaa cggcaaattc
     2701 gattaccttt acgataaagt gggtttgtat gataaattgc gtggtatcat gagccgtaac
     2761 gaacccgccc gtgacattac agctcagtgg caaagtctga acggaataca gaaccaaatg
     2821 ctcaatttcc ttgaaaacca cgacgaacaa cgtattgctt cgggtttttt ctgtggcagc
     2881 ggcgtctatg cccagccagc catgattatc tccgccacac tgacccaatc tcctgtgatg
     2941 atttacatgg gtcaggaact cggcgaactg ggcatggata tggaaggttt cagcggtgtg
     3001 gacggcaaaa catcaatttt cgactactgg ggtgtggcct caatccaagc ctgggccaac
     3061 ggtggaaaat ttgacggagc tcaccttcat caggaacaga aagaactgcg tgctttctat
     3121 caacaactgc taaacctatg catgagcgaa aaagcaatca cagacggagt gatgtacgat
     3181 ctggtgtttg ctaactttgg aaatccgaat tttaatacac acgaacaatt tgcctatttc
     3241 agaaaatata aaaaagaatt acttttgtgt gtcgtaaatt ttcaggataa gaacatcggg
     3301 atggagataa atatccccac tgaagcattc cagttcctgg atctggatga aaatgaattt
     3361 tacagttcgg tgaatttgct caaccgtgaa gaggatttcc ccattcagcg cttgtcgtcc
     3421 ctcaatcctt accgggtctt tctaccggca tggagtggca aaattctgaa actgaaacat
     3481 gaaaaaatag tttgacacag tagtaaaaag caacaaaata tcataacatt aaaaaaaaca
     3541 attatgagta aaattgaaaa aatcgtagcc cgcgaagtcc ttgactcccg cggaaatcct
     3601 acagtagaag tagatgtaac ccttgaatcg ggtgtagtgg gtcgtgcatc agttccatca
     3661 ggagcctcca ccggcgaaaa cgaagccatt gaacttcgcg atggcgacaa acaacgtttc
     3721 cttggaaaag gagtactgaa agctgtaaaa aatgtaaatg aagtgattgc tcctgccctt
     3781 atcggcgaat gcgcactcca acaacgcaaa atagacaaag caatgatcgc tttggacggt
     3841 accaaaacca aatcgaatct gggtgctaat gctatcctgg gcgtatcgct ggctgtggcc
     3901 aaagctgctg ccatctacct ggatatgccg ctctatcgct acattggcgg aacgaatgct
     3961 ttcactctgc ctgttccaat gatgaacatc attaacggtg gttctcactc cga
//
LOCUS       DAOPZK010000166         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_29717_length_3989_cov_22.848754, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000166 DAOPZK010000000
VERSION     DAOPZK010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29717_length_3989_cov_22.848754"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKH68_08335"
     CDS             <1..408
                     /locus_tag="PKH68_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX12985.1"
                     /translation="LQPEACAEDEFLLENAHQSARMFGLPYTEAVQPIGKYIVEGDEI
                     RFGNSSFSCLAVPGHSPGSLAFYNQQEGIVLTGDALFNGSIGRTDLARGDYATLIKSI
                     QEKLLTLPDNTVVYSGHGPATTIGNEKENNPYL"
     gene            complement(501..1043)
                     /locus_tag="PKH68_08340"
     CDS             complement(501..1043)
                     /locus_tag="PKH68_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNX12986.1"
                     /translation="MRIISGTFKARRIDTPHNITARPTTDFAKEGLFNVLNNLIDFED
                     IDVLDLFAGTGSISLEFISRGANNVVSVEKNDLHCTFIRKTCTLLGVDNLLLTRGDVF
                     RFLSSSHMKFDVIFADPPYDLQQLPLLPDLIFKHDLLKPEGLFILEHSAKNNFENHPN
                     FLEHRHYGNVNFSFFKMKTQ"
     gene            complement(1045..1851)
                     /locus_tag="PKH68_08345"
     CDS             complement(1045..1851)
                     /locus_tag="PKH68_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNX12987.1"
                     /translation="MKIITGYCRPEAVQSSEDYHLTQRNFLKAMSEKSLLNSADNTLE
                     INISPESISYAVSDDNQQVLLRQELNIPIGPATDMGVFEHFFNQPELQILGENVRIHY
                     ESNQYQLIPHELFSEKDMADFFSVEHGASENEAVQYQILPKWGAILTYRLPVRMMAFF
                     RQKFPEAEMEHHIALLLKKQLFNRETACWLNVRKDIIDILVMQNKHLQLVNSFEVKTH
                     EDCCYFVLNVYEQLQLDTETFHLNVIINKSVNEELIKLLKKYIKNVENIK"
     gene            2041..>3989
                     /locus_tag="PKH68_08350"
     CDS             2041..>3989
                     /locus_tag="PKH68_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNX12988.1"
                     /translation="MNTQNYQLYRKLLRSHSPLLSMDEKREMRALLKSFYSRKTSPEL
                     LSDEYLFTKLQILDIVLNEIGLGKPAVVSVLFIEAVDDKFITPEELNKKFNTSITPIM
                     YGLQRVKELYQKNVALETENFRKLLLTLAEDVRVILIIVAIRLQTMRNLDKLPDAEKQ
                     QIARESAFLYAPLAHRMGLYKVKTELEDLSLKYTNHDIYKEIAKKLNETKRSRDKYIS
                     EFIDPLKLKLADIGCEFEIKGRTKSIHSIYSKIKKQNTAFEKIYDLFAIRIILDTPLE
                     KEKAACWQAYSIITDMYQPNPNRLRDWLSIPKSNGYESLHTTVMGPQGKWVEVQIRTV
                     RMDEVAEKGLAAHWKYKGIKSESGMDEWLKNIREILENPELNAVDFMDEFKLNLYDKE
                     VFVFTPNGDLHKLPKGATALDFAFSIHSNLGTRCVGAKVNGKNVPIKYVLNNGDQVEV
                     LTSSSQKPNQAWLKIATTSKAKGKIRQALKEVEYKDAEIGRETLLRRFKNWKIEVDDG
                     RFSRLSKKQGFKTVSDFYQAIAQETIDIHGIRDAYLDMEARDNNTNIEIRTAENYVKE
                     IAPLPETTSKEDVLVIGENLKNIDFRLAKCCNPIYGDDVFGFVSVTGGIKIHRTDCPN
                     APQMISRFGYRIVKARWSGKSIGSQ"
BASE COUNT         1201 a          883 c          847 g         1058 t
ORIGIN      
        1 cttcagcctg aagcatgcgc agaggatgaa tttttgctgg aaaatgccca tcagtcagcc
       61 cgaatgtttg gtttacccta cacggaggct gttcagccaa taggaaaata cattgtggaa
      121 ggggatgaaa tccgatttgg caacagcagt ttcagttgtc tggcagtgcc cggtcactca
      181 cccggaagtt tggcttttta taatcagcag gaaggaattg ttctcaccgg cgatgcgtta
      241 ttcaatggat caatcggcag aacagacctt gcccgcgggg actatgccac actcataaaa
      301 tctattcagg aaaaattgct tacattaccc gacaacacgg tggtgtacag cggacatggt
      361 cctgcaacca ctattggtaa tgaaaaggaa aataatccgt atttgtgacc cccgacgcct
      421 aaagaggaat aaggaagagt gaaatttttt taaaaaagtt cactcttttt ttataactaa
      481 gcgtaaaaaa tcacattttt ctactgtgtt ttcattttaa aaaaactgaa attcacattg
      541 ccgtaatgcc tgtgttccag gaagttcggg tgattttcaa agttgttttt ggcggaatgt
      601 tccagaataa ataatccttc gggtttgagc aaatcatgct tgaaaatgag atcgggcaat
      661 agaggcaact gctgcaaatc gtagggcgga tcggcaaaaa tcacatcaaa tttcatgtgc
      721 gaggaactta gaaaacgaaa cacatcaccg cgtgtaagta aaagattatc cacacccaac
      781 agcgtgcagg ttttgcgaat gaaggtacaa tgaaggtcat ttttctccac actgactacg
      841 ttgttggctc cccgcgaaat aaattccaga ctgatgctgc ccgttccggc aaacaggtca
      901 agcacatcaa tgtcttcaaa atcaatcaga ttgttcaata cattaaacaa cccttccttg
      961 gcaaaatctg tagtgggtcg tgccgtaata ttatggggtg tatctatcct tcgggcttta
     1021 aatgttccgc ttattatccg catcttattt tatgttttct acgtttttga tatacttttt
     1081 caacaatttg ataagttctt catttactga cttattaatt attacattta aatgaaatgt
     1141 ttcggtatcg agctgcaatt gttcgtacac attcagcaca aaatagcaac aatcttcgtg
     1201 ggttttaact tcgaacgaat tgaccagttg caggtgctta ttttgcatca ccaggatgtc
     1261 gattatatcc ttgcggacat ttagccaaca agctgtttcc cggttgaaaa gctgtttctt
     1321 gagcagtaaa gcgatatgat gctccatttc ggcctccgga aatttttgcc ggaaaaaggc
     1381 catcattcgc accggcagcc gataagtaag aatggctccc catttgggca ggatttgata
     1441 ctgcacggct tcattttccg aggctccgtg ctcaacagaa aaaaaatcag ccatgtcctt
     1501 ttccgaaaaa agttcgtgag gtatcagttg gtactgattg ctttcgtagt gaattcggac
     1561 attttcgccc aaaatctgta attcgggctg attgaagaaa tgttcaaaca cgcccatatc
     1621 agttgccgga ccgataggaa tattcagctc ctgccgtagc agcacctgct gattgtcgtc
     1681 cgaaacagca taggagatac tttcggggct tatatttatt tccaacgtat tgtcggctga
     1741 atttaaaagt gatttttcgg acatagcttt aagaaaattt cgttgtgtca aatggtagtc
     1801 ttcgctcgat tgcacagctt ctggccggca ataaccggtg attattttca tacaaaatta
     1861 acaaagaaaa ttcaatctga gctcaaccta ccccatttct tttcattttt ccgaattttt
     1921 acgtttttca acgatcattt ttttcagcct ttgggtatga agttgtatat ttgcactatc
     1981 tgcaagtggt tatatcaatc ccattcagtt tacacattgc tcctctaaca ggtctcagtt
     2041 atgaatacac aaaattatca actataccgc aagctgcttc gctcccactc cccgctcttg
     2101 agcatggatg agaagcggga gatgcgagcg ttactaaaaa gtttctacag ccgaaaaact
     2161 tcaccggaac tgctatcgga tgaatacctg ttcaccaaac tgcagatact ggatatcgta
     2221 ttgaatgaaa tcggtctggg caaacctgct gtggtgagtg ttttattcat cgaagccgtt
     2281 gatgataaat ttattacgcc cgaagaactt aacaagaaat tcaacacatc tatcacaccc
     2341 atcatgtacg gcctgcagcg tgtgaaagaa ctgtatcaga aaaacgtagc cctcgaaaca
     2401 gaaaattttc gaaaactgct gttgaccctg gccgaagatg tacgggttat ccttatcatt
     2461 gtagccatcc ggctgcaaac catgcgtaat ctggataaac tgcccgatgc cgaaaagcag
     2521 cagatagccc gcgaaagtgc atttctgtat gctccgctgg ctcatcgtat gggactttac
     2581 aaggtaaaaa ccgaactgga agacctttcg ctcaagtaca ccaaccacga tatttataag
     2641 gaaatagcca aaaaactgaa cgagacgaaa cgttcacgtg acaaatacat ttcggagttc
     2701 attgacccac tgaaactgaa actggcagat attggatgcg aatttgaaat caagggacga
     2761 accaagtcca ttcattccat ttattccaaa ataaaaaaac aaaacacagc gtttgaaaaa
     2821 atatatgacc tttttgctat tcgcatcatt ctggatactc cgctagaaaa agaaaaagct
     2881 gcttgctggc aggcctactc catcattacc gacatgtatc agccaaatcc caaccgcttg
     2941 cgcgactggc tttcaatccc aaaatccaac ggctacgaaa gcctccatac cactgtgatg
     3001 ggtcctcagg gcaagtgggt ggaagtacag atacgtaccg taaggatgga cgaagtggcc
     3061 gaaaaaggac tggcggcaca ctggaaatac aagggtataa aaagtgaaag tggaatggat
     3121 gagtggctga aaaacatccg tgagatactg gaaaatcccg aactgaatgc cgtggatttt
     3181 atggatgagt ttaagctgaa tttgtacgac aaggaagttt ttgtattcac gcccaacggt
     3241 gacctgcaca aattgcccaa aggagcaacg gcactcgatt ttgcattctc catccactcc
     3301 aacctgggaa cgcggtgcgt gggtgccaaa gtgaacggaa aaaatgtacc cattaaatat
     3361 gtgctgaaca acggcgatca ggtggaagtg ctcacttctt cgtcacaaaa gcccaatcag
     3421 gcctggctga aaatagcaac aacctccaaa gccaaaggca aaatacgcca ggctctcaaa
     3481 gaagttgaat acaaggatgc cgaaataggt cgcgagacac tgcttcgccg gttcaaaaac
     3541 tggaaaatag aagtggacga cggacgattt tcacgcctgt ccaaaaagca gggtttcaaa
     3601 actgtcagcg atttttatca ggctatcgct caggagacca ttgacataca cggaattcgc
     3661 gatgcatacc tggacatgga agcccgcgac aacaacacca acattgaaat cagaactgcc
     3721 gaaaactatg ttaaagaaat agctccgtta cccgaaacca cttcaaagga agatgtgctt
     3781 gtcatcggtg aaaatctgaa aaacatcgat ttcaggctgg ctaagtgctg caatcccatt
     3841 tatggcgatg atgttttcgg ctttgtatcc gtcacgggtg gcatcaaaat tcaccgtaca
     3901 gattgcccca atgcacccca aatgatatcc cggttcggtt accgcattgt caaggctcgc
     3961 tggtcaggca agtccatcgg gtcacaata
//
LOCUS       DAOPZK010000167         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_29788_length_3981_cov_18.448803, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000167 DAOPZK010000000
VERSION     DAOPZK010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29788_length_3981_cov_18.448803"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..2051
                     /locus_tag="PKH68_08355"
     CDS             6..2051
                     /locus_tag="PKH68_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX12989.1"
                     /translation="MAVQLLPAQQKPAQKWLDQKFSLFIHFGLYSQYGGVYDDKPVTR
                     GYSEQIQSFAGIFSDWYADAAREFNPVNWNPDSIVALVKKAGMRSIVFTSKHHDGFCM
                     YQSKYTDFNIVDATPYKRDLMKELAEACKRGGIDFAVYFSLIDWHFPQAYPISSHNAD
                     PLTPEHYTFNLKQVEEIMTNYGPISEIWFDMGSLTAEQSRGLYELVNRLQPECMISGR
                     LGNNYVDFSVMADNEYPDYKIGVPWQTAASMFDETWGYRSWQERGNAQDKVNEKIASL
                     IKVISRGGNYLLNIGPRGDGSVVEFEKEVLLKMGAWTNANAEAIYATKANPFPHAFSW
                     GDITTRDNSLYLFVRKDNQAIDLNGFNGKITDVSVLASGEKCRYQQNLKSNSVQISLP
                     ENPENIPVTVLKVSFEDRFTVLPKDIVSTGLLTAENAVPEFGHSSADYYCGYKSLTGY
                     GWHFRKSGSQLSPTVFFTENEQGKNIRFTLDGKTIETSLNPQDSTVVKTKNAFAWGNQ
                     YRKKGKGVFGYVEEENRLSPEIDTTWKNIGTMKYGERQQMELRSRESLLVLQEIQSSA
                     NQDAAIEISSGCAAYIILNGKYITAYFPTPGVEFQKKILVLPVKKGNNQLIIKFFNRN
                     TEELMYSLKPLEKWTVYRMKLPSFNLNSTATHQFNVKAANPPSKVAPLEMSNIRVRM"
     gene            complement(2837..>3981)
                     /gene="pckA"
                     /locus_tag="PKH68_08360"
     CDS             complement(2837..>3981)
                     /gene="pckA"
                     /locus_tag="PKH68_08360"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNX12990.1"
                     /translation="NYGEPDFVCLNGSKTTNPNWKEQGLNSEVFTLFDLTRKMQVIGG
                     TWYGGEMKKGIFALMNYYLPLRGIASMHCSANVGTEGDVAIFFGLSGTGKTTLSADPK
                     RYLIGDDEHGWDDHGVFNYEGGCYAKVIDLEKDKEPDIWRAIRRDALLENVTVLPDGT
                     PDYSAAASKTENTRVSYPIYHINKIVSPSRAGHAKKIIYLSADAFGVLPPVSILDEQS
                     AQYHFLSGFTSKLAGTERGITEPVPSFSPAFGEAFLTLHPTMYAKTLIGKAKEHNAKV
                     YLVNTGWNGTGKRISLKNTRAIIDAIIDGSIENAPTVHIPILNLVAPTALNNVDTEIL
                     DPRNTYADVAEWETKAKSLAAKYIKNFEQYCDNDDAKALVASGPQL"
BASE COUNT         1141 a          880 c          935 g         1025 t
ORIGIN      
        1 tgcttgtggc agtccagctt ctgccggcac agcaaaaacc cgcacagaaa tggctcgacc
       61 agaaattctc cctgtttatt catttcggac tttattcaca atacggaggc gtgtacgacg
      121 acaaaccggt cacccgcggt tacagcgaac aaattcagtc ctttgcggga attttcagcg
      181 actggtatgc cgatgcagca cgcgagttca atcctgtgaa ttggaacccc gacagcattg
      241 tagctctcgt taagaaagcc ggcatgcgct ccatcgtttt cacctccaag caccacgacg
      301 ggttttgcat gtatcagtcg aagtacaccg attttaatat tgtggatgcc acaccttaca
      361 agcgggattt gatgaaggag ctggccgaag cctgcaagcg cggaggaatt gattttgccg
      421 tgtatttttc gcttatcgac tggcattttc cacaggccta ccccatttcg agccacaatg
      481 ccgacccgct cactcccgaa cattacacgt tcaacctcaa acaggtggaa gaaatcatga
      541 ctaattacgg acccatctcg gaaatctggt ttgatatggg atcgctcacc gcggagcaaa
      601 gccgcggact gtatgaactg gtcaaccgcc tgcagcccga atgcatgata agcggacggc
      661 tggggaataa ttatgtggat ttcagtgtga tggccgataa tgagtatcct gattacaaaa
      721 tcggagtacc ttggcaaaca gccgcttcca tgttcgacga aacatggggc taccgctcct
      781 ggcaggaacg gggaaatgcg caggacaaag tgaatgagaa gattgcaagc ctgataaaag
      841 tgatcagccg gggtggtaat tacctgctca atatcgggcc gcggggcgat ggaagtgtgg
      901 tggaatttga aaaggaagtg ctgctgaaaa tgggtgcatg gacaaacgcc aatgccgaag
      961 ccatctatgc caccaaagcc aacccttttc cgcacgcatt ttcgtggggc gacattacca
     1021 caagggataa cagcctgtat ctgtttgtca gaaaggataa tcaggcaatt gatctgaatg
     1081 gatttaacgg aaaaataacc gatgtttccg ttctggcatc gggtgagaaa tgcaggtatc
     1141 aacaaaacct gaaaagcaat tcggtacaaa tttctcttcc ggaaaatccg gaaaatattc
     1201 ccgtgactgt gctgaaagtg agtttcgaag atcgctttac cgtactacca aaagatatcg
     1261 taagcaccgg tctgcttact gccgaaaatg ccgtaccgga attcggtcat tccagcgcgg
     1321 attactattg cggttacaaa agtctgacgg gttatggctg gcatttccgt aaatcagggt
     1381 ctcaactctc cccgaccgta tttttcaccg aaaatgagca aggaaaaaac atccggttta
     1441 ccctcgatgg aaaaacgata gaaacatcct taaatcccca agattctacg gtagtgaaaa
     1501 ccaaaaatgc attcgcatgg ggaaatcaat accggaaaaa aggaaaaggg gttttcgggt
     1561 atgtggaaga agagaaccgg cttagcccgg agattgatac gacatggaag aatattggaa
     1621 caatgaaata cggagaaagg caacaaatgg aacttcggtc gcgcgaaagc ctgttggttt
     1681 tgcaggaaat ccaatcttct gccaaccagg atgcagccat cgaaatcagc agcggctgtg
     1741 cggcttatat tatattgaac gggaagtaca tcacagcgta tttccccaca cccggggttg
     1801 aattccagaa gaaaattctt gttttaccag taaaaaaagg caataatcag ctgatcatta
     1861 aatttttcaa ccgaaataca gaggagctga tgtatagctt aaaaccgttg gagaaatgga
     1921 ccgtttaccg gatgaaatta ccttcattta acctgaacag caccgcaact catcagttca
     1981 acgtgaaagc agccaatccg ccgtcaaaag tcgcgccatt ggaaatgagc aatatacggg
     2041 taaggatgta gttgccccaa gcccctcccg aaaaatcggg ataaatgccg gggatgttaa
     2101 gaccattcgg gtaaaatgga gttgccccaa gcccctcccg ataaaaatcg ggataaatgc
     2161 cggggatgtt aagaccattc gggtaatatt ttaatatgaa tattcgtttg gctaccagat
     2221 ccttttgaat taagatatca gggctacgcc cctaaaaatc aatttatggt cattgtacta
     2281 caaacctgca agcagcaggc tgggatatca cagctatgct gttcattagc aacgtcaatt
     2341 atttactacc atacggccgc ctctacgagg ctacattttc agcaacaata atattctatc
     2401 ctgatattgc atctacgagg ctgtgtttcc agcacccgag gtgcgacagt atggtagaaa
     2461 atgaattaga ttaacattat agagctccgg aggtgcgaca ttatcgtttt attaaaaatc
     2521 aacgaaaaaa tgtttcggta cgctgtacct taaaaaccct ggataattca ttttctacaa
     2581 acctgcatgc tgcaggcagg tgttacgcca cgctgtggct taaattcagg tgcagcgcac
     2641 cgtgacattt gtagatgaat agttcagaat aatttattca ggtacaaatg gtacattccg
     2701 acttgacgga agtaccgaaa cattttggag ttaaactttt caacaattcc aattcacata
     2761 cataaaaaaa atcccgcctc cattacgaag cgggattttt aaagtccccc ttgaggggga
     2821 tttagggggc ttgttcttac aattgtggtc ctgaagcaac caatgctttt gcatcgtcgt
     2881 tgtcgcagta ctgttcgaag ttcttgatgt atttagcagc cagtgattta gctttggttt
     2941 cccattcagc tacatcagca taggtgttac gtgggtcaag aatttcagta tccacattgt
     3001 tcaacgctgt aggagcaact aagttcagaa ttggaatatg tactgttggt gcattttcga
     3061 ttgaaccgtc gatgatggcg tcaatgatgg cacgggtgtt tttcaacgag atacgttttc
     3121 ctgttccgtt ccagccggtg ttaaccagat acacttttgc attgtgttct ttagctttgc
     3181 caatcaatgt ttttgcatac atggttgggt gcaaagtcaa gaaagcttca ccaaatgcag
     3241 gtgagaaaga aggaaccggt tcagtgatac cacgttctgt tccggctaat ttggaagtaa
     3301 agcctgaaag gaagtgatat tgtgctgatt gttcgtcaag aatagatacc ggaggcaata
     3361 caccgaatgc gtcagccgac aggtaaatga ttttcttagc gtgtcctgca cgtgaaggag
     3421 aaacgatttt gttgatgtgg tagattggat aagaaacacg ggtgttttca gtttttgaag
     3481 cagctgctga atagtcagga gttccatcag gaagcactgt aacattttcc agcaaagcat
     3541 cacgacggat agctctccag atatcgggtt ctttatcttt ttccaggtcg ataaccttgg
     3601 catagcaacc accttcgtag ttgaacacac cgtgatcatc ccagccgtgt tcgtcatcac
     3661 cgatgaggta acgtttcggg tcagcagaaa gggtagtttt acctgttccc gaaagtccga
     3721 agaaaatagc cacatcacct tctgtaccta cgttggccga gcaatgcatg gaagcgatgc
     3781 cgcgcaatgg caggtagtaa ttcatcaggg caaaaatacc ttttttcatt tcgccgccat
     3841 accaggttcc accgattacc tgcattttgc gggtcaaatc gaacaaggta aacacttcag
     3901 agttcagtcc ctgttctttc cagttagggt tggtggtttt tgaaccgttc agacatacga
     3961 aatcaggttc gccgtagtta g
//
LOCUS       DAOPZK010000168         3914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_30567_length_3914_cov_25.842446, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000168 DAOPZK010000000
VERSION     DAOPZK010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30567_length_3914_cov_25.842446"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKH68_08365"
     CDS             complement(<1..488)
                     /locus_tag="PKH68_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNX12991.1"
                     /translation="MHKITTIILLCCAFLMTATDISAQNADFKLLKSINSNPSTALRG
                     YSRFVTNTTTGITLATPAVMGVVGLIKKDDELLKNAIFVSAALGVDGVLTYSAKKAFH
                     RQRPYLSYPDDIIAYETEKSLSFPSAHTSLAFSTATALSLSYPKWYVIAPGYFWACSV
                     GYS"
     gene            566..1108
                     /locus_tag="PKH68_08370"
     CDS             566..1108
                     /locus_tag="PKH68_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX12992.1"
                     /translation="MIKIALTGPESTGKTELSLQLAEHFHAALEPELAREYVENLDRD
                     YTFEDVCDIARLQVEQELKYENIEDENALVIFDTDLIITKVWLDYKFGYVPPFVTERL
                     SHRFMDLYLLCEPDLPWVPDPVREHGDDRPFFYDWYEREIIQLATPYVKISGTGDDRF
                     VNARQAVEDFILAEKNKKIL"
     gene            1230..2108
                     /locus_tag="PKH68_08375"
                     /pseudo
     CDS             1230..2108
                     /locus_tag="PKH68_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
     assembly_gap    2463..2562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2599..3735
                     /locus_tag="PKH68_08380"
                     /pseudo
     CDS             2599..3735
                     /locus_tag="PKH68_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
BASE COUNT         1190 a          797 c          875 g          952 t
ORIGIN      
        1 gaatagccta ccgaacaggc ccaaaagtat ccgggtgcaa tcacgtacca tttcggataa
       61 ctgagactta atgcagtagc agttgaaaat gccagtgaag tgtgagccga tggaaacgac
      121 agcgattttt cggtttcgta ggctatgatg tcatccgggt aggaaagata tggccgctgg
      181 cgatggaatg cttttttagc cgaataggtt agtactccgt caactccaag agcagcactt
      241 acaaagattg catttttaag taactcatca tcttttttaa tcaacccaac aactcccata
      301 accgcaggtg tagcaagcgt aatgccggtg gtggtattgg taacgaaccg tgaatacccc
      361 ctgagtgccg ttgaaggatt gctattaatg cttttaagaa gcttaaaatc tgcgttttgt
      421 gccgaaatat ccgtggctgt catcaaaaaa gcgcaacaaa gcagaataat tgttgtaatt
      481 ttgtgcattc cttttttttg attgattgta actgatacaa atgtaatatt tattctgaat
      541 aataagaccc aacttcttta attccatgat taaaattgcc ctgacaggac cggagtcaac
      601 cggaaaaact gaattgagcc tgcaactggc agagcacttt cacgctgccc tggagcccga
      661 actggcacgc gaatatgtgg aaaatctcga tagggactat acctttgagg atgtgtgtga
      721 cattgcacgc ctccaggtgg aacaggaact gaaatatgag aatattgagg atgaaaacgc
      781 cctggttatt tttgataccg atttgataat tacaaaagta tggctcgact ataaatttgg
      841 atatgttccg ccatttgtta ctgaaagatt atcccatcgc tttatggacc tctatctgct
      901 ttgtgaaccc gatttgccct gggtgcctga tccggtaaga gagcacggcg atgaccgccc
      961 gtttttttat gactggtacg aacgtgaaat cattcagctg gcaacacctt atgtgaaaat
     1021 cagcggaaca ggcgatgatc gtttcgtgaa tgccaggcag gcagtggagg attttatttt
     1081 agcagaaaaa aataaaaaaa ttttgtagaa ataaaagatt tgtaagaaca gttcgtttgc
     1141 ccgattgttc tttctttcga taattcagta ctaaaaatat aatttgccag acttctacag
     1201 gcaaactaca tcagtcatac agcatcacaa tgaataagca aattgaaaaa agcatcgaac
     1261 ttctctcgca tcccgatagc ctgaactacg tctgccgcca gcaggaagaa aatcatccca
     1321 tgccatccac cgatgttatc gggcgggtag tgcaactatg ccgttcagta atttttccgg
     1381 ggtatttcgg caattcggat gtcaatcccg ataatgtgaa cgaacacatc gaacagaata
     1441 taatactgct tcaccagttg ctcacccagc agattaaatc gggcatgtgt ttcgataatt
     1501 caactgtaga ttatacctgt aaggcgctcg aagcacagtc catacagatt gccgccgatt
     1561 ttgtaagtca gctgcccgcg atacgtgaaa tacttcatac cgatgtaatt gcggcttttg
     1621 atggtgatcc tgcagccaaa agttatggcg aggttatttt ctgttacccg ggcattcgcg
     1681 ctatcagcag ttaccgcatc gctcatgccc tgctcaaact gggagttccc gttattcccc
     1741 gtattattac cgagaaggcg cattccgaaa caggtatcga cattcatccc ggagctgtta
     1801 ttggcgattt ctttaccatt gaccacggaa caggggtcgt tattggggaa acagccgtaa
     1861 ttggcagcaa tgtgaaaatg tatcagggcg taacgctggg agcaaaaagt tttccgctgg
     1921 atgaaaacgg aaatcccatc aaaggcattg accggcatcc aaaaatcggc aacaacgtga
     1981 taatatacgc caattccact attttgggaa atattaaagt gggcgatgac tcggttattg
     2041 gtggaaatat atgggtagat accgatgttg ctcccggtac cagactgttg caacgcaagc
     2101 gggaggaata ccacgattta gctaccggag atcatgcttc gaaaaagaaa aaacacaagg
     2161 aagaggaata ccgggaagaa aaaaaaagag ataaggaaca aagacataaa aaagaaaagg
     2221 acagggaagt aaaagacaag gacgagaagc gcaaagaaaa ggtaaaatac gcacctgcca
     2281 acctgagtgg caaatataca atggtctttg aaaaatgcaa aggctgcaaa agccgtaacg
     2341 gctgtgccaa atgccgggct aaatgggaaa agaaacacgg gaagataatt cagtagaaag
     2401 ttcatcaagt agaaagttca tcaagtagaa agttcatcaa gtagaaagtt catcaagtag
     2461 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaactttac aaaactaaca
     2581 cgaactaaat atacaggaat ggaatttgaa atgatagcca agaccttcaa gggtctggaa
     2641 gaagtgctcg caacggaact cgtggcaatt ggagccaata atgtgcaact tcagcgacga
     2701 gcagtgagtt ttaccggtaa tcaggaactg atgtacaaag ccaatctgca attgcgaaca
     2761 gcgtcgaggg tgttgaaacc tatcgccact ttcaaagcgc ttaatcccga tgaagtgtac
     2821 gaacaggtga aaaatattaa atgggaaaaa tatatgaccg tcgataccac atttgccatc
     2881 gattcgacta tattctctga tgaatttcgc cattccatgt ttgttgctta ccgggtgaaa
     2941 gatgctattg ccgaccggtt tatggaaaag gagaacaaac gaccctccgt gagactggat
     3001 aatccgcaac tgatgatcaa tgtacatata gctcaaaacc agtgtaccat atcgctcgac
     3061 agctcgggtg agtcgcttca caagcgcggc tatcgggtgg ctcaaacaga tgcaccactc
     3121 aacgaagccc tggctgccgg aatgctgctg atgagtggtt gggagggaaa aaccgatttt
     3181 gtagatccga tgtgtggctc aggcacgtta ctgatcgaag cagcattaat tgcgctgaac
     3241 ataccaccgg gaattttccg taaagagttt gcttttgaga aatggatgga tttcgatgca
     3301 gagttgtttg acgcggtgta caacgacgat agcgtggaac gtccttttga gcacaaaatc
     3361 tacggctcgg atatttcgcc cctggccatt aaaatagccg ataaaaacgt gcgcagtgca
     3421 ggtcttaata aatacatcga actgcaggta ctgcctgtac agcaactcga attgccctcg
     3481 cctcactgtc tgatggtgac caatcctccg tatggcgaac gcattacctc acccgattta
     3541 ttcggactgt atgcagcact cggtacggta ctgaaacgta aatttgccgg aagttcggca
     3601 tgggtcatca gttcacacga agaatgtctg gataaaatcg gattaaaacc aagccgcaag
     3661 attcctctcc tgaacagtac gctggaatgt ctgtattgcc agtatgaaat cttcgaagga
     3721 aagagaaatg attttgtagc cgaaaaaaag aaacgctacc gaaactaagc ttcaacagta
     3781 ataactgcaa aataatttca gtaaatggca aaaaatcttt gtcagttcca aaatttagtt
     3841 tacttttgca cccactaaac gcccagatgg cggaattggt agacgcgctg gtctcaaaca
     3901 ccagtggatt cact
//
LOCUS       DAOPZK010000169         3869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_31087_length_3869_cov_22.509963, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000169 DAOPZK010000000
VERSION     DAOPZK010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31087_length_3869_cov_22.509963"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PKH68_08385"
     CDS             complement(<1..493)
                     /locus_tag="PKH68_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNX12993.1"
                     /translation="MRYGLITLMLLIHVLAFGQKSGSISGKVLDSENNIVEFVNVFLT
                     SVNDSTTIVNGTVTDNTGSFDLTNVPLGKYFIQFRFIGFLNHQQTVILDDVSKNIDLG
                     TIIMKQDAIALNAIEITAFRNLIQRTDEGIVVNASENLTQIGGTAADLMKNMPGVQVD
                     MEGN"
     gene            complement(504..1481)
                     /locus_tag="PKH68_08390"
     CDS             complement(504..1481)
                     /locus_tag="PKH68_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNX12994.1"
                     /translation="MNSKIILTTAITLISTVLFAQTPKAYITNYGSDFISVIDIQKNQ
                     KIADITTGSKPHGVAVSPDGNWIAVSNEGDNTVSIISAADNKVKQTISVGKTPHQLSF
                     SIDGKYVLTAINGEHKVDVISTANWKLVNSIPVGKNPHIVLPMSDGKTAWVTSEGDNK
                     IVKVNLDSMKVVAEIPTFGFPRVMTATPDGKYIYLTIRWFHGQVKIDAEQNKIIAQVL
                     LPQSDKFDNEGKAAHGLSVAPDGKTVYLTSQFTNDVTAIDVASDKILKNITAGKNPNW
                     IELTSDGKFAVVSNTSSNDASIIDVANWKVVATIPVGKNPKRLWVANTK"
     gene            complement(1556..2728)
                     /locus_tag="PKH68_08395"
     CDS             complement(1556..2728)
                     /locus_tag="PKH68_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNX12995.1"
                     /translation="MDEVLHNRKINLLAYLKTNPEAPFQGDNPLDDFTFYAIDAATFQ
                     KSKENYTDTLIYEPVDDELDEYRKLNTFVEIHNQYYKLEILAPHLEADEMLGTIAFFL
                     GILLTGLSLSFYLSQRIISRKIWKPFYDMLEKLRGFRLDKQQLPELSSSQIEEFKMLN
                     ESINELVLKNMEVFTSQKQFIENASHEMQTPLAVMQSRLEALIGRAELTKEQAEIVEG
                     LISSTQRLKKLNKTLLLLSKIENRQFLLTDQVDVNQIIRQSMEYYEEQKSKLNISVTI
                     KSETQLTVQGNIMLTEILIQNLLKNAFLHNTENGILTIYVGEKQLTISNSGHKKDEEG
                     SMLDKEKLFNRFYKQSGNPDSWGLGLSIAKKIADTSGWELCYRSDGALHIFEVNFQ"
     gene            complement(2836..3519)
                     /locus_tag="PKH68_08400"
     CDS             complement(2836..3519)
                     /locus_tag="PKH68_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX12996.1"
                     /translation="MKLLIVEDEPQLLKSLEDFASKEGYLYDSSQGFCSAAERIALYD
                     YDCIVLDINLPDGNGFDILEALHKEGKTNGVIILSARDSLDDKLKGLGLGADDYLTKP
                     FYFSELNARIKAIIRRKQFKTNKIVHFSNLIIEPDQHLIGVSNLDNPISFTKKEFALL
                     SYLINNHKRVLSKVSLAEYIWGDYVDEAQSFDFLFSQIRNLKRKLKEAGADVEINNVY
                     GIGYQIKAR"
BASE COUNT         1080 a          743 c          730 g         1316 t
ORIGIN      
        1 aatttccttc catatcaacc tgaaccccgg gcatattttt cattaagtcg gctgccgtac
       61 cgccaatttg tgtcaaattt tccgatgcgt tcaccacaat tccctcatct gttctctgaa
      121 tcaggttgcg aaaagctgtt atttcaatag cgttcaatgc gattgcatcc tgtttcatta
      181 taatggttcc caaatctata tttttgctta catcgtcaag tattactgtt tgttgatgat
      241 ttaagaatcc gataaaccga aactgaataa aatatttacc aagggggaca ttcgttaaat
      301 caaaactgcc cgtattgtct gtaaccgttc cgttaacgat agtcgttgaa tcgttaacgg
      361 aagtaagaaa aacattgaca aattcaacaa tattgttttc gctgtctaat actttgcctg
      421 atatgcttcc tgatttttgc ccaaaagcca atacatgtat taacagcatt agtgtaatta
      481 atccatatct cataaatcaa caattatttt gtatttgcca cccacaaacg ttttgggttt
      541 ttcccaaccg gaatagtcgc caccactttc caatttgcaa catcaataat actggcatca
      601 tttgatgaag tgttgcttac aactgcaaat ttaccgtcag aagttagttc tatccagttt
      661 gggtttttgc cggctgtaat atttttcagg attttatccg atgctacatc aatagccgtt
      721 acatcgttag taaactggct cgtcaggtaa acggttttgc cgtcaggagc tacagaaagt
      781 ccgtgggctg ctttcccttc gttatcaaat ttgtccgatt gcggtaaaag aacctgggct
      841 atgattttgt tttgctccgc atcaattttt acctgtccgt gaaaccagcg aatggtcaga
      901 taaatatatt ttccgtcagg tgtagcggtc atcactctcg gaaatccgaa agttggaatt
      961 tctgctacga ctttcatgct gtccagattc acttttacga ttttattgtc tccttcggaa
     1021 gttacccaag ctgttttccc atctgacata ggcaatacaa tgtgtgggtt tttgccaacg
     1081 ggaattgaat tgaccaactt ccaattagcg gttgaaatca catcaacctt atgttctccg
     1141 ttgatggcgg ttaaaacata cttaccgtca atgctgaaac ttaactggtg aggtgtctta
     1201 ccaacggaaa tggtctgttt tactttattg tcggcggcac taataataga aactgtatta
     1261 tcgccttcat tgcttacggc aatccaattt ccgtcaggtg aaacggctac cccatgcggc
     1321 ttgctccctg tagtaatatc ggcaattttt tgatttttct gaatgtcaat aacagaaata
     1381 aagtcgctgc catagttggt aatgtaagct tttggcgttt gagcaaaaag cactgtacta
     1441 attagtgtta ttgctgttgt caagattatt ttgctgttca tttttcaata gttttaaatg
     1501 atttacctta caaaggaaac cattgaatct gtagaaaaat gggaatcgga actgtttatt
     1561 gaaaattcac ttcgaagata tgcaaggctc catcacttcg gtaacaaagt tcccaaccgc
     1621 tggtatctgc aattttttta gctatggaca atccgagacc ccacgaatca ggatttcccg
     1681 attgcttata aaaacggtta aacaattttt ctttatccaa cattgagcct tcttcatctt
     1741 ttttgtggcc tgaattggaa atagtaagct gcttttctcc tacataaatg gttagtatgc
     1801 cattttctgt attatgcaaa aaagcatttt taagcaggtt ctgaataaga atttccgtga
     1861 gcatgatatt gccctgtact gtcagttggg tttctgattt aatcgttact gaaatattaa
     1921 gttttgattt ctgttcttca taatattcca ttgactgccg tataatctga tttacatcaa
     1981 cctggtcagt taacaggaac tgacgatttt caattttcga tagtaaaaga agggttttat
     2041 ttagtttttt taaacgttgg gtggagctta taagtccttc cacaatctct gcctgctctt
     2101 ttgtcaactc tgcccgccct attagggctt ccagtcgtga ctgcattacg gcaagtggtg
     2161 tttgcatttc atgggaggca ttttcaataa attgtttttg actggtaaat acttccatat
     2221 ttttcaaaac caattcatta atggactcat taagcatctt aaattcttca atctgagaag
     2281 atgataactc tggaagctgc tgcttgtcga ggcgaaaacc acgaagtttt tccaacatat
     2341 cgtagaatgg tttccaaatt tttcgtgaaa ttattcgctg tgataaataa aaagagagtg
     2401 atagcccggt aagcaaaatt ccaagaaaaa aggcaattgt gcctaacatt tcatcggctt
     2461 caagatgcgg tgctaaaatc tccaatttgt aatattggtt atggatttct acaaaagtgt
     2521 ttaatttcct gtattcatcg agttcatcat ctacaggttc ataaattagc gtatctgtat
     2581 agttttcttt gcttttttgg aaagttgcag catctatggc ataaaaagta aaatcgtcca
     2641 gcgggttatc accttgaaat ggagcttcag gattggtttt gaggtatgca agcaggttaa
     2701 tttttctatt atgcaacacc tcgtccacat tccgaagtac attccagtta agcactacgt
     2761 aaaacagaat ggaaaaaatt ccaaacagaa tcatgaaata acgcaactga atccgtgagg
     2821 tgtaggtaag tagcttcatc gtgcttttat ttgataacct attccataaa cattgttaat
     2881 ttctacgtct gccccggctt ctttcagctt acgttttaag ttccttatct gtgagaaaag
     2941 gaaatcgaaa ctttgggctt catccacata atctccccag atatattctg ccagtgatac
     3001 cttggaaaga acccgtttat gattattaat gagatagcta agtaaagcga attctttttt
     3061 agtaaatgaa atggggttat caaggttgct tacaccaatg aggtgttggt ctggttcaat
     3121 aatcaggtta gaaaagtgta cgattttgtt tgtcttaaat tgctttctac ggataatggc
     3181 tttaattctg gcatttagct cagagaaata gaatggtttg gtaaggtaat cgtcggcacc
     3241 cagtccaagc ccttttagtt tatcatctaa tgaatcacgt gctgaaagta ttatgacccc
     3301 atttgtcttg ccttctttat gcagggcttc aagtatatcg aaaccatttc cgtcaggaag
     3361 gttaatatca agtacaatgc aatcgtagtc gtaaagagcg atgcgttcag cagcagaaca
     3421 aaatccttgg gaagaatcat acaggtatcc ctctttggat gcaaaatctt ccaaactttt
     3481 taataattgc ggttcgtctt ctacgataag taatttcata aatacaaatt aagggataaa
     3541 ttctgtagaa acattggaga tattttatgc ctgataatat tctctaaaat gtccgagggt
     3601 tcctggtggt aggctgcact ctttgacaaa gctttggttt cagcgttggc tcgtggggct
     3661 ttggcaatgt gtgcaatgtg ggtggggttt tattcttttt tgcgggtggg aagaaaaaaa
     3721 caaaataaat ttggctccgc cgagctccac ttcgttccgg ttccatcaaa tttgcactgt
     3781 cctgtcaaaa agctaaatct tttctttcgt tattatttgt cactattgta tctgtctggt
     3841 cgtctgtctt tttacacttg ttgccaacg
//
LOCUS       DAOPZK010000170         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_31328_length_3848_cov_23.160032, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000170 DAOPZK010000000
VERSION     DAOPZK010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31328_length_3848_cov_23.160032"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PKH68_08405"
     CDS             complement(<1..792)
                     /locus_tag="PKH68_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HNX12997.1"
                     /translation="MKLQFLLFISFFSLLCQGQVVKGFQQLSDKVNVTLADGILSISP
                     LSDNAVRIKFFKLSEGDLPEFVFASETATPDFRVVDFSSRLEIKTSKITVLIDKSNGK
                     LTYADNEGNMFLSEKADSRKLTPGSIQGEPCFAVEQSFESPADEYIFGLGQFQDGQYN
                     LKNVTRRLTQVNSQIAIPFIYSSKGYGLLWHQYGLTDFNPADNFISLEQQQSTTGNDQ
                     TAEVTTTSGTQKVSQNQSLYHGKFNVSVDGEYSIFLDLGSMGNRHF"
     gene            complement(946..3390)
                     /locus_tag="PKH68_08410"
     CDS             complement(946..3390)
                     /locus_tag="PKH68_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX12998.1"
                     /translation="MKKINLVIFIFLLSIGAYAQSFTKTVLGVKTQINSTDVEIQFYG
                     PSTVRVLKSPAGTAFTKESLAVIKKPQTTKLSVQQQGDVILLKSEKLKVDINLKSGKI
                     AFETLSGTSLLSEKESGVKFTDFNDAGSKTYTVSQLFKLDADENIYGLGQQQRGKLSL
                     RNEKINMVQGNTDDYVPFLVSTKGYGLFWDNYSPTVFEDKPESASFKSEVGDCIDYYF
                     MLGGNIDGSIACMRDLTGQAPMFPLWTFGFWQSKERYKSQSELVGVVQKHRELGVPLD
                     GIIQDWQYWGSNYLWNAMDFLNPEFPNPKKMVEDIHNMNAHVIISIWNSFGPQTKQYR
                     EMKPQGMLLKFGTWPQSGLETWPPNMEYPSGVEPYDVYNPAARQIYWKYLKNGLFSLG
                     IDGWWMDSSEPDHLDFKPTDFDIKTYLGSFRKVRNAFPLMTVGGVADNQRATSSDKRV
                     FILTRSAFAGQQRYGANTWSGDVNSSWQSLRNQIPAGLNFSMSAIPYWNTDIGGFFAG
                     AYNKSWNDGSGAKNPAFQELYVRWLQFGAFTPMMRSHGTDVPREIYNFGKKGEPIFDA
                     IAKSINLRYSLLPYIYSAAWDITNNQSTMMRALVMDFNDKKVVDMNNEYMFGKSILVA
                     PIVNAQYTSETVVKTDENSGWNKDDKKDEKAIAVDFTQQKSSKAYLPAGTSWFDFWSN
                     QKYDGGQEVTLTTTIDKIPLFIKAGSIVPFGPNVQFATEKKWDNLEIRVYPGANAEFT
                     LYEDENDNYNYEKGIYSTISFTWNDAKKSLTINDRKASFPGMLATRKFNILLISSGKK
                     VEKTVTYSGKKVSVKL"
     gene            complement(3454..>3848)
                     /locus_tag="PKH68_08415"
     CDS             complement(3454..>3848)
                     /locus_tag="PKH68_08415"
                     /inference="COORDINATES: protein motif:HMM:NF023010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HNX12999.1"
                     /translation="GMINVGESKRGVRRVIPITGGTFSGPKIKGEVLPGGEDWQLVRP
                     DGDTELYARYLLKTDDGHVIQVINNALIHTDAKTKAFYCKSVLDLEAPQNSPYDYLNH
                     AIFIGTLNMPALKQGEEPYVIIGVYKLL"
BASE COUNT         1111 a          831 c          744 g         1162 t
ORIGIN      
        1 aaaatgacga tttcccatac tccccaaatc caaaaatatt gaatactcgc cgtccacaga
       61 cacattgaat tttccatggt acaacgattg gttttgagat actttctgag taccggaagt
      121 tgtcgtaact tcggctgtct gatcatttcc cgtagtggat tgttgttgtt cgagtgaaat
      181 aaaattatct gccggattaa aatcggtaag tccatactga tgccaaagca aaccataacc
      241 tttactggaa tagataaaag gaattgcaat ttgcgagttc acctgcgtca accgacgggt
      301 aacgtttttc aggttgtatt gtccgtcctg aaattgcccc aggccgaaaa tatactcatc
      361 agcgggcgac tcgaaactct gctcaaccgc aaaacagggt tcaccctgaa tagacccggg
      421 cgtgagtttt cgtgaatctg ctttttcact cagaaacata ttgccctcat tgtcagcata
      481 agttagtttt ccattgcttt tgtcaatcaa cactgtgatt tttgaggttt ttatttcgag
      541 cctggaagaa aaatcaacca cccgaaaatc aggagttgcc gtttcggaag caaagacaaa
      601 ctcgggtaaa tcaccttcgg aaagtttaaa gaactttatc ctcaccgcat tgtccgacaa
      661 aggcgaaata ctgagaatgc catccgccaa tgttacattt accttatcag acagctgctg
      721 aaatcctttc actacctgtc cctgacatag tagagaaaaa aaactaatga ataagaggaa
      781 ttgtaatttc atgcgtaaat aattaacata tctgccccct aatccgtcag ctgacggagg
      841 aatattgagc gtctatcaaa tgtcgtgagt tttttaatca tgcacgtgtg tgtaaaaaat
      901 attttacctg gacatgtgtg ttttatggcg ttagttataa ttcttttaca atttaactga
      961 aacttttttt ccactgtagg ttaccgtttt ctccactttt ttaccgcttg aaataagtag
     1021 aatattaaat ttacgggttg caagcattcc cggaaacgaa gctttacgat cattgattgt
     1081 caatgatttt ttggcatcat tccatgtaaa agatattgta gaataaattc ctttttcgta
     1141 gttgtaatta tcattttcgt cttcgtacaa agtgaattca gcgttagctc cggggtaaac
     1201 acgaatttcg agattatccc atttcttttc ggtggcaaac tgtacgtttg gaccaaatgg
     1261 cactatgcta cctgctttga taaataatgg aattttatct atggttgttg taagcgtaac
     1321 ttcctgtcca ccatcatatt tttggttcga ccagaaatcg aaccacgaag ttccggccgg
     1381 aagatatgcc tttgacgatt tctgttgcgt aaaatcaaca gctattgctt tttcatcttt
     1441 tttatcatct ttattccaac ctgaattttc atcggtttta accaccgttt cggacgtata
     1501 ttgagcattt acgattggag caactagtat tgatttacca aacatgtatt cgttgttcat
     1561 atcaacaacc ttcttatcgt tgaaatccat caccaaagcg cgcatcatag tggattggtt
     1621 gttagttata tcccaggctg ccgagtagat ataaggcaat aaactgtagc gcaaatttat
     1681 acttttcgca atggcatcga agatgggctc ccctttttta ccgaaattat aaatttcgcg
     1741 gggtacatct gttccgtgag agcgcatcat tggcgtaaat gctccaaact gcaaccaacg
     1801 aacataaagt tcctgaaaag cagggttttt agctccggaa ccatcattcc agcttttgtt
     1861 atatgcgcct gcaaaaaaac caccaatatc cgtgttccag taaggaatag cactcatcga
     1921 aaaattgaga ccagccggaa tttgattgcg aagcgattgc catgacgaat tcacatcgcc
     1981 cgaccaggta ttggctccat agcgttgttg tccggcaaaa gcagaacggg taaggataaa
     2041 tacacgctta tctgaactgg tggcacgctg attatcagca actccaccca ctgtcattaa
     2101 tggaaatgca ttacgcactt tgcggaatga gccaagatat gttttaatat cgaaatctgt
     2161 tggtttgaag tccaaatgat ccggttcaga agaatccatc caccagccat cgattcccag
     2221 agagaaaagt ccatttttca gatatttcca gtaaatttgg cgagcagcag gattatacac
     2281 atcataaggc tcgacacccg atggatattc catgttgggc ggccacgttt ccagtcccga
     2341 ttgcggccat gtgccaaatt tcagcagcat accttgtggt ttcatttcgc ggtattgctt
     2401 tgtttgagga ccaaacgagt tccagataga aataatcaca tgcgcattca tattgtgaat
     2461 atcttccacc atctttttag gattcgggaa ttcaggatta agaaaatcca tagcattcca
     2521 caaataattg ctcccccaat attgccagtc ctgaataatg ccatcgagag gaactccaag
     2581 ttcgcgatgt ttttgaacca cacctacaag ctcactttga ctcttgtaac gttccttact
     2641 ttgccaaaag ccaaaagtcc acaagggaaa catgggcgct tgtccggtaa ggtcgcgcat
     2701 gcaagcgata gagccatcga tatttccacc aagcataaaa taataatcga tgcaatcgcc
     2761 cacctccgat ttgaatgaag cactttcggg cttatcttca aaaacagtag gagagtagtt
     2821 gtcccagaat aacccataac ctttggtcga aacaaggaaa ggaacgtaat cgtcggtatt
     2881 gccctgcacc atattgattt tttcgttacg caacgagagt ttaccccgtt gctgttgccc
     2941 taaaccataa atattctcgt cagcatcgag cttgaacaac tgacttactg tatatgtttt
     3001 tgaaccggca tcattaaaat cggtaaattt cacacctgat tccttttcac tcaaaagcga
     3061 tgtacctgaa agcgtttcaa aagctatttt gcccgatttc agattgatat ctactttcag
     3121 tttttcgctt ttaagcaaaa taacatctcc ttgttgttgg acagacagtt tggtagtctg
     3181 tggcttctta atcactgcca aactctcttt tgtgaaagcc gttccggcag gcgatttgag
     3241 tacccgcacc gtagatggtc cgtaaaactg gatttcaaca tctgttgaat tgatttgggt
     3301 tttgactccc aaaaccgttt ttgtaaacga ttgagcataa gctccaatgc ttaacaaaaa
     3361 gatgaatatc actaaattta tttttttcat aaatcatcga ctttttaaaa atttaacaaa
     3421 gaaaacttgg agaactggta acaatgcgca catttacaat aacttataaa cgccgattat
     3481 aacataaggc tcttcgccct gtttcagagc gggcatattt aatgtgccga taaaaatagc
     3541 atgattaaga taatcgtaag ggctattttg aggggcttct aaatcaagaa cagatttgca
     3601 gtaaaaagcc ttggttttgg catcggtgtg aattaatgca ttattaatga cctgaataac
     3661 atgaccatcg tctgttttca ggaggtagcg ggcataaagt tcggtgtcgc catcagggcg
     3721 gaccagttgc cagtcttcac cgccgggtaa aacttctcct tttattttcg gaccgctgaa
     3781 cgttcctccg gtaatgggaa taacccttct gacgccacgt ttgctttcgc ctacgttgat
     3841 cataccgg
//
LOCUS       DAOPZK010000171         3841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_31417_length_3841_cov_21.389857, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000171 DAOPZK010000000
VERSION     DAOPZK010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31417_length_3841_cov_21.389857"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /gene="rfbB"
                     /locus_tag="PKH68_08420"
     CDS             complement(<1..941)
                     /gene="rfbB"
                     /locus_tag="PKH68_08420"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNX13000.1"
                     /translation="MGKTILITGGAGFIGSHVVRLFVTKYPEYQIVNLDALTYAGNLE
                     NLKDIKDAPNYTFVKGDITDETFIPELFGKYSFDGVIHLAAESHVDRSITDPFAFIRT
                     NVFGTANLLNAAKNAWKGSMDGKRFYHISTDEVYGSLGSEGFFTEETAYDPRSPYSAA
                     KASSDHFVRAYYHTYGLPVVVSNCSNNYGSHHFPEKLIPLAINNIKKKLPIPIYGKGE
                     NVRDWLWVDDHARAIDVIFHKGVTGETYNIGGHNEWTNIDLIKELCAIMDRKLNREPG
                     ESAALITYVKDRAGHDLRYAIDSSKLQRELGWVPSLQ"
     gene            1071..1994
                     /locus_tag="PKH68_08425"
     CDS             1071..1994
                     /locus_tag="PKH68_08425"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13001.1"
                     /translation="MEQIARHIETLLRRHDYVVVPDLGGFVVQNQQAVIHDDAIEPPL
                     SRVGFNPLLNVSDGMLTIEISRAEKISFREASQLIEKSVAGLKTALKAQKTVSCGNLG
                     TLSVDAEEKIIFKPSLNADMLPSNYGLGTLHYSPFAESIAAEDRKLTITLPSPRKIAR
                     YAAIGLIAASVFLAAPTLNDARQNFAGLYPENLLETSEKPVSTNINTPTPLAAVSNTT
                     IAETKEIEATPAEEARFHVVVASLPTQEMADKYCNELKADKFTKVHVLPPARLFRVVV
                     ESFTTKDSAVVFMENLRNSSPEFAEAWVLCE"
     gene            2091..2666
                     /locus_tag="PKH68_08430"
     CDS             2091..2666
                     /locus_tag="PKH68_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNX13002.1"
                     /translation="MPTKIRLQRHGRKGYAYYHIVIADSRAPRDGKFIERIGSYNPNT
                     NPATIDLKFDRALYWVNAGAQPTDTTRNILSDEGVLLKKHLQGGVKKGAFSEEEAEKR
                     FAAWKANKENAVSKTKEQLAAEKQAKAKERLKAEADVNKAKAAELAKKKAEELESKME
                     AAKAELKSTVAEETNTEAAVEAPATETPAAE"
     gene            3016..>3841
                     /locus_tag="PKH68_08435"
     CDS             3016..>3841
                     /locus_tag="PKH68_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNX13003.1"
                     /translation="MNLSDIKKHITSKVDKSELAKRLYKGISWTLIGNIIGKFLQLVA
                     FIFVARIVGKEEYGQVGIIRSTLNMFLLFSSAGMGATATRYIAIYRTSDPNRAYQIYR
                     FSLKVVLAIGFAVSLIVFLMSDHIAEKYLNDVNLGDALRVGAFTLFLMTLTSVQTGAL
                     NGFERFRSLGINLAANGLILFASVIVGAYLRGVNGVVAGLAISNLIYAVQLWFSLKND
                     ILGIRHAEQRPNIERLSKSSVFLKFSLPAVLESVTIVAVLWWAKTFLIGIEGYGEMA"
BASE COUNT         1176 a          777 c          825 g         1063 t
ORIGIN      
        1 aattgcagtg aaggcaccca accgagttcg cgctgaagtt tcgatgaatc gatggcgtaa
       61 cgcaggtcat gtccggcacg atctttaaca taggtaatca gtgcggcgga ttcacccggt
      121 tcgcggttga gtttgcggtc catgatggca cacagttcct ttatcaggtc aatgttggtc
      181 cattcgttgt gtccgccgat gttataggtt tcgccggtta cacctttatg aaatatcaca
      241 tcaatggctc ttgcatgatc gtccacccaa agccagtcgc gtacattctc tcctttgccg
      301 taaatgggta tcggtagttt tttcttgata ttgttgatgg ccagcggaat taatttttcg
      361 ggaaaatgat gagaaccgta gttgttggaa cagttggaaa cgacaacagg caggccataa
      421 gtatggtaat aggcacgtac aaaatggtcg gaactggctt tggcagccga atacgggctg
      481 cgtggatcat aagctgtttc ttcggtgaaa aaaccttcac ttccgagaga gccgtacact
      541 tcatcggtgg aaatatggta aaaccttttt ccgtccatgc tgcctttcca ggcatttttg
      601 gcggcattca gcaggttggc cgtgccaaag acattcgtac gaataaaagc gaagggatcg
      661 gtaatggaac gatcaacatg cgattcggca gccaggtgaa tgactccgtc gaatgagtat
      721 ttgccgaata attccggtat aaacgtttcg tccgttatat cgccttttac aaatgtataa
      781 ttcggagcat ccttgatgtc tttcagattt tccagattac cggcataggt cagcgcatcc
      841 agattaacaa tctgatattc ggggtatttg gttacaaaca aacgtaccac atgcgaaccg
      901 ataaacccgg ctcctcctgt tattaaaatg gtttttccca taatatttct attgtgaata
      961 ttgatttgat acggacaaag atacagtctt tgggtgatat ttaaaaactt gttgaaaact
     1021 ttttcagtag aaattattgt ttcattcaaa aaagatttac ttttgcatga atggaacaaa
     1081 tagcccgtca tatagaaacc ctgctgcggc gacatgatta tgttgttgtg ccagatttgg
     1141 gtggttttgt tgtgcaaaat caacaggctg tcattcatga cgatgccatt gagccaccac
     1201 tatccagagt aggtttcaat ccgttgctga acgtttcgga tggtatgctg accatcgaga
     1261 tctcgcgtgc tgagaaaatc agtttccgcg aagcatctca actgatcgaa aaatcggttg
     1321 ccggactgaa aaccgcatta aaagctcaaa aaaccgtcag ttgcggcaat ctgggcactt
     1381 tatcggtgga tgcggaagaa aaaatcattt tcaaaccatc actcaacgcc gacatgctgc
     1441 cttccaacta tgggctgggc acattacact attcaccctt tgcagaaagt attgcagcgg
     1501 aagaccgaaa actgaccatc acactaccct caccccgaaa gattgcccgc tatgcggcca
     1561 tcgggctgat agcagcaagt gtatttctgg ctgcacccac actgaatgat gcccgtcaga
     1621 attttgccgg attatatccc gaaaatctgc tggaaacttc cgagaaaccg gtttcaacca
     1681 acatcaacac tcctactccg ctcgcagcgg taagtaacac gacgatagct gaaaccaaag
     1741 aaatcgaagc cactcccgca gaagaagcgc gatttcatgt agtagtggcc agtctgccaa
     1801 ctcaggaaat ggccgataaa tactgtaacg aactgaaagc tgacaaattt acaaaggttc
     1861 acgtacttcc acccgccaga ttattcaggg tagttgtaga atcattcacc acaaaagatt
     1921 ccgctgtggt ctttatggaa aatctgcgaa atagctctcc cgaatttgca gaagcctggg
     1981 tactttgcga ataatcatcc gattattttc cgtttccaat aaaaaatctt atctttgcat
     2041 ccgcttttcg agaaaaagca tcaggtataa tcatttaaaa aataaaaaaa atgccaacaa
     2101 aaatcagatt gcaacgtcac ggccgcaaag ggtatgctta ttaccacatt gtgattgctg
     2161 acagtcgcgc gccacgtgat ggcaagttta tcgaacgaat cggttcgtac aatccgaaca
     2221 ccaatcccgc taccattgat ctgaagttcg acagagcttt gtattgggta aatgctggtg
     2281 ctcaacccac agacacaacc cgcaacatcc tctctgacga aggtgtgctg ctgaaaaagc
     2341 acctgcaagg tggtgttaaa aaaggagctt tcagtgaaga agaagccgaa aaacgcttcg
     2401 ctgcctggaa agcaaacaaa gaaaatgctg taagcaaaac caaagaacag ttagcagccg
     2461 aaaaacaggc aaaagctaaa gaacgtctga aagccgaagc tgatgtaaac aaagcaaaag
     2521 ctgccgaact ggccaagaaa aaagcagaag aattagaatc taaaatggaa gctgccaaag
     2581 ccgaactaaa atcaacagta gccgaagaaa caaataccga agccgcagtt gaagctcccg
     2641 caacagaaac acctgctgct gaataaatta cctgcttaag atatttattt tagaaagccc
     2701 gaatagataa gttcgggctt ttttgttaaa acaaatactt gtgagcaatt ttagtgttaa
     2761 tatttcgtta cgattttaat attgacgggt cattttattt attatcattt tgcatttccg
     2821 gccattgtgg cagcgaaaaa ggagtaataa tttatcatta aacagggaat acacattcat
     2881 tgacaaccaa cttattgaca atactttata cgaatatccg atattttttc atagaatgtt
     2941 atttttaatt aagattatcg gacactacac cacaaaatca ataactgcgt gaaagtagaa
     3001 ttataatcta ggagcatgaa tctatccgat atcaaaaaac acattacaag caaggttgat
     3061 aaatctgaac ttgcaaaaag gctttataaa ggtatttcgt ggactttaat aggtaatatt
     3121 atcggaaaat ttttgcagct ggtagctttt atttttgtag ccagaatagt tggtaaagaa
     3181 gaatatgggc aggttggcat tattcgttca acgcttaaca tgtttctatt attttcatct
     3241 gcaggaatgg gggcaacagc tacgagatat attgcaatat acagaacatc agatccaaac
     3301 agagcatacc agatttaccg gttttccttg aaagtggttt tggcaatcgg ttttgctgta
     3361 tctttaatag ttttcttgat gagcgaccat atcgctgaaa aatacttaaa tgatgtcaac
     3421 cttggagatg cattgagagt aggagctttt acactttttc taatgacttt gacatcggtt
     3481 caaacgggag ccttaaatgg ttttgaacgg ttcagaagcc tcggaataaa tttagctgca
     3541 aatggattga ttttatttgc ttctgtaata gttggggctt atttaagagg agttaatggg
     3601 gttgttgcag gactggcaat atctaatctg atttatgctg tacaactgtg gttttcctta
     3661 aaaaatgata ttctggggat acgtcatgct gagcaaaggc caaatattga aagactgagt
     3721 aagtcatccg tttttctgaa attctcttta ccggcagttc tcgaaagcgt aacgattgtt
     3781 gccgtattgt ggtgggcaaa aacgttttta attggcatag aaggatacgg tgaaatggcc
     3841 a
//
LOCUS       DAOPZK010000172         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_31790_length_3809_cov_22.798615, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000172 DAOPZK010000000
VERSION     DAOPZK010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31790_length_3809_cov_22.798615"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1271
                     /locus_tag="PKH68_08440"
     CDS             <1..>1271
                     /locus_tag="PKH68_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNX13004.1"
                     /translation="PIYPTISQEDYRHILNHAGMKMIFIEGKDLRSRLEPILPTLTTL
                     EEIFTFNQENSKYRILDQLIDLGRQKPQPEELAKIKADIRPEELATIIYTSGTTGAQK
                     GVMLSHSNIVNQLLSLSVTPAEWSNKALSFLPLCHAYERTLVYLYQYLGMSVYYAESL
                     GTIAENIKEINPTMMTCVPRLLEKIYDKLYASGRKLPFPKRNIYYWAFDLATKYQLQG
                     MSKLMEYQLKLADKLIYSKWREAIGGNFDIVVSGGSAIQPHMASFFSAIGMPVFEGYG
                     MSEGSPVIAVSQRGVNGRKFGTVGPPLPGVNIRIIENNEIVFRGHNVMMGYYKDPELT
                     AQVIDKDGWLHTGDTGKFTPEGQLVITGRLKNIFKTSFGKYVNPQAVESKFSESGFFE
                     NLIVLGENKKYAAALILPDFAYLRTWCGEHK"
     assembly_gap    1272..1371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1430..2248
                     /locus_tag="PKH68_08445"
     CDS             1430..2248
                     /locus_tag="PKH68_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNX13005.1"
                     /translation="MIKQKKNPKILFISQEIFPYLPESEIANQGRYLPQAAQERGKET
                     RTFMPKFGLVNERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARMQVYFIDNDD
                     YFQRKNTITDDNGVEYDDNDERTIFYVRGVMETVKKLRWTPDIIHCQGWMTALAPLYI
                     KKAYNEDPFFKNSKVVYSLFNDEFSKPFHAQFAESLLLEGIEKNHLTSITGKEIDYVA
                     LSKLAIDHSDGVIQSSQTVHPALVKYVAEKKMKFLPYVDPEDYADDYLNFYDSL"
     gene            2304..3692
                     /locus_tag="PKH68_08450"
     CDS             2304..3692
                     /locus_tag="PKH68_08450"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HNX13006.1"
                     /translation="MKFNFIFSLLLLISVVFFSCDDSALNLGENIQPTSDKISIHADT
                     FHLSTETFLVDHIYSTPDSFLLGTYIDNVLGTTRGDILTQFYYPVNYKFLDTSVATAT
                     PDSVKLILSFGSYFGLNTSPMEISVYELKDSLRMKENYRSDINTAQYVNFSKKLNQED
                     NGIFTVYNVKNKTNNSSVSIKLTDEFLNRFFTTNSATYSSDLNFRNFFKGLYITTDFG
                     STTLLNVSGVYLMLYYHNTYVKDGSKASVSLTFPASHLEVKRVNRILHPTRPLNFSAA
                     DEINYVASPANYYTRVKIPVERMRNRLILPAGKHRIINSALLRVNVVDKDTIGTRLPY
                     VKNMLLVKEDDLEDFFLNNKLPTSGSSYLVSLDSTNITSTTYKYHYNFGTLSSLIESE
                     LKQPAASHKDGYVNMVLVPVDVYKTTSGSYYSTSTTINEVRQNTQLQAVSIYSGKNKK
                     IPMKLEVVYSGF"
BASE COUNT         1143 a          830 c          770 g          966 t
ORIGIN      
        1 cccatttatc ccaccattag tcaggaagac taccggcaca tactgaacca cgccgggatg
       61 aaaatgattt tcatcgaagg aaaagacctt agaagtcgcc tggaacctat tttacccaca
      121 ctgacaacat tagaagaaat tttcactttc aatcaggaga actcgaagta cagaatactg
      181 gatcaattga ttgaccttgg aaggcaaaaa ccacaaccgg aagaactggc caaaatcaaa
      241 gcggatatta gacccgaaga actggctaca atcatatata catcgggaac taccggtgca
      301 caaaaagggg tgatgctttc gcacagcaat atcgtaaatc agctgctcag cttgagtgta
      361 acacccgccg agtggagcaa taaagcactg agttttctgc ctctctgtca tgcctacgaa
      421 cgcacactgg tatatctgta ccaatacctg ggtatgtcgg tgtactatgc cgaaagcctg
      481 ggcaccattg ccgaaaacat caaggaaatt aatccgacga tgatgacctg cgtaccacgg
      541 ctgctcgaaa aaatatatga caaactttac gcttccggac gtaaactgcc tttccccaag
      601 agaaatatat attactgggc atttgatctg gctacaaaat atcagttaca agggatgagt
      661 aaattgatgg agtatcagct caagctcgcc gacaaactga tatactccaa gtggcgtgaa
      721 gccatcggcg gcaacttcga catagtagtt tcgggcggat cggctataca gccccacatg
      781 gcttctttct tttcggccat tggtatgccg gtgttcgaag gctatggcat gagcgaagga
      841 tcgccggtga tagctgtgag ccagcgtggc gtgaacggac gaaaattcgg cacggtagga
      901 ccaccactgc cgggagtgaa tattcggatt attgaaaaca atgaaattgt attccggggg
      961 cacaatgtaa tgatgggata ctacaaagat cccgaactta ctgcacaggt aatagacaaa
     1021 gacgggtggc tgcacaccgg cgatacgggc aaatttaccc ctgaaggaca actggttatc
     1081 accggaaggc tgaaaaatat tttcaaaacc tcatttggaa aatatgtcaa tccacaggct
     1141 gtagagtcta aattttcaga atcgggattt tttgaaaatc tgatcgtact gggtgaaaac
     1201 aaaaaatatg cagccgcact catcctgccc gattttgcgt atctgagaac ctggtgtggt
     1261 gagcataaaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natttccgat
     1381 tttttttata tatctttgca agtgaattgt atttaaacac ccagcaagga tgattaagca
     1441 gaaaaaaaat ccaaaaatct tatttatttc tcaggaaata tttccctatt tacccgaatc
     1501 ggaaatcgca aatcagggtc ggtatttacc tcaggcagcg caggaacgcg gtaaagagac
     1561 acgtacgttt atgccgaaat tcggcctggt aaacgaacgc cgcaaccagt tgcacgaagt
     1621 aatccgcctc tctggcatga acctgattat agacgatacc gatcatccgc ttatcatcaa
     1681 ggtagcctcg atacagtcgg ctcgaatgca ggtttatttt attgataacg atgattattt
     1741 tcagcgaaaa aatacaataa ccgatgataa tggtgtggaa tatgatgaca atgatgagcg
     1801 aaccattttc tatgtacgcg gggtgatgga aacggtaaaa aaactgcggt ggacgccgga
     1861 tataattcat tgtcagggat ggatgaccgc tttagcccct ttgtacatca aaaaagcgta
     1921 caacgaagat ccgtttttca aaaattcaaa agtggtatat tcactgttta atgacgaatt
     1981 cagcaaaccg tttcatgctc agttcgccga gagcctgctg ctcgaaggca ttgaaaagaa
     2041 ccacctgacc agcatcacag gcaaagaaat tgactacgtt gcactctcga aactggccat
     2101 tgatcactcc gacggagtta tacagtcgag ccaaacggta catccggctc tggtgaaata
     2161 tgtagctgag aaaaaaatga aatttttacc ctatgtagac cccgaagatt atgccgacga
     2221 ttatctgaat ttttatgaca gtctttaaaa aaccggtatt ctttcaacaa aaaaaaatgt
     2281 tttacatcta atttactcac taaatgaagt ttaatttcat attttcgctt cttttactca
     2341 tttcagttgt gtttttttcg tgcgatgact cggcattgaa cctgggcgaa aatatacagc
     2401 caacctccga taaaatctct atacatgcgg atacgttcca cctgagcacc gaaacattcc
     2461 tggttgatca tatatattcc actcccgatt catttctgct cggaacttac atcgataatg
     2521 tgctgggtac cacccgcggt gatatcctta cccagtttta ttaccctgtc aattacaaat
     2581 ttctggatac ttcggtagct acggcaaccc ccgattcggt gaaactcata ctctcgtttg
     2641 gttcttattt cggactgaac acctcaccga tggaaatcag tgtgtacgaa ctgaaagatt
     2701 cgctcagaat gaaagaaaac taccgttcgg acatcaatac tgcccaatat gtaaatttca
     2761 gcaaaaagtt aaaccaggaa gataacggaa tttttacagt gtataatgta aaaaacaaga
     2821 caaataattc gagcgtatcg atcaaactga ctgatgaatt tctgaatcgc ttcttcacta
     2881 ccaattccgc aacgtacagc agcgacctta acttccgcaa ttttttcaag ggactctata
     2941 tcaccaccga ctttggcagc accacactgc tcaatgtgag cggcgtttat ctaatgcttt
     3001 attatcacaa cacctacgtg aaggacggaa gtaaagcctc cgtatcactt acatttccgg
     3061 ccagtcacct ggaagtgaag cgtgtaaacc gcatattgca cccaacccgt cccctcaatt
     3121 tttcggcggc tgacgaaatc aactatgtgg cctcaccggc caactattat acccgggtga
     3181 aaattccggt ggaacgaatg cgaaaccgcc tgattttgcc agctggtaaa caccggatta
     3241 tcaatagcgc tttgcttcgt gtgaatgtgg ttgataagga tacaatcggc acccgattgc
     3301 cttatgtgaa gaatatgttg ctggttaagg aagatgatct ggaagatttc ttcctgaaca
     3361 acaaactccc cacttcggga agttcgtatc tggtaagtct tgacagcacc aatatcactt
     3421 ctactaccta caaatatcat tataacttcg ggaccttgag ttcgctcatc gagtctgaac
     3481 taaaacaacc ggctgcaagc cacaaagacg gttatgtgaa tatggttctg gtgcctgtgg
     3541 atgtgtacaa aaccacctcc ggttcatatt attcaacgag caccaccatc aacgaagttc
     3601 gccagaatac tcaactgcag gcagtaagta tttatagtgg taaaaacaaa aaaatcccca
     3661 tgaaactgga agtggtgtac agcggtttct aatgggaatc gcagtaaaac ttcatcttac
     3721 ctaaaacaaa acagcagaac cgattggttc tgctgttttg ttttaggtgg ttgacaacta
     3781 attcaatgac tatttctgcc ggataaata
//
LOCUS       DAOPZK010000173         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_32997_length_3716_cov_20.172904, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000173 DAOPZK010000000
VERSION     DAOPZK010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32997_length_3716_cov_20.172904"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..334)
                     /locus_tag="PKH68_08455"
     CDS             complement(62..334)
                     /locus_tag="PKH68_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13007.1"
                     /translation="MEDNFDVCWQCNFSISENKVIEFEEPTVPEKKINCLRCNVPMML
                     SGNYKFHEGTKFGIFGNLLEAFVNRESFDLYICPNCGKVEFFSPQG"
     gene            complement(376..2376)
                     /locus_tag="PKH68_08460"
     CDS             complement(376..2376)
                     /locus_tag="PKH68_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNX13008.1"
                     /translation="MKKTGLTLLVSLALCNFVFGQKNFTLSSPDGKVKAEINIGKTIE
                     YSVFHQTDLLVSKSPVSLRFTDGTSPGINAKLKKATKKSVNEVIDAVIYKKNKISDNY
                     NELILSFRGDYNLIFRAYNDGVAYRFSTDRKKPFTVENEQAEFNFPGDPKVYAAYVND
                     DNGRKDTFEKQFFNSFENQYQHIALSSWNPKRLAFLPLVIEGKNGKKICITESDLLDY
                     PGMYLHGNGSGGLAGVFAPVPRDIKPGGHNMLQGLVQSREPYIAVCEGKTNFPWRAVI
                     VAENDCELTNSDMVYKLATPSQLSDISWIKPGKVAWDWWNDWNIKNVDFQTGVNNATY
                     KYYIDFASKYKIEYVILDEGWAVNLKADLYQIVPEIDLKELIAYAKSKNVDLILWAGY
                     NAFNKDMEGICRHYSEMGIKGFKVDFMDRDDQPMVDFYHRAAQIAAKYHLMLDFHGAF
                     KPAGLQRTYPNVINFEGVHGLEQMKWSPSSVDQVTYDVTIPFIRMVAGPMDYTQGAMR
                     NATRDNYRPVNSEPMSQGTRCRQLAEYVIFESPLNMLCDNPSAYMEEKECTEFIAEIP
                     TVWDETVALNGEIAKYITIARRKGNVWYLGSMTNWDARKLDLDLSFLGEGDFRAEIFR
                     DGVNAHRAASDYRKLTVDIPADRKMQISMAPGGGFVMKIMKK"
     gene            complement(2393..3343)
                     /gene="ftsY"
                     /locus_tag="PKH68_08465"
     CDS             complement(2393..3343)
                     /gene="ftsY"
                     /locus_tag="PKH68_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNX13009.1"
                     /translation="MGLFGLFNKEKKETLDQGLSKTKESVFSKLTRAVAGKSKVDEEV
                     LDNLEEVLVSSDVGVETTLRIIERIEERVSKDKYLNTSELNKILKEEIAALLAENNSG
                     QPTDFDDELPATPYVIMVVGVNGVGKTTTIGKLAYQFKKAGKKVYLGAADTFRAAAVE
                     QLVIWGERVGVPVVKQKMGADPASVAYDTLASAKANNADVVIIDTAGRLHNKINLMNE
                     LTKIRNVMQKITPDAPHEILLVLDGSTGQNAYEQAKQFTLATDVNAIAVTKLDGTAKG
                     GVVIGISDQFKIPVKYIGLGEKMTDLQVFDKEEFVASLFE"
     gene            complement(3571..>3716)
                     /locus_tag="PKH68_08470"
     CDS             complement(3571..>3716)
                     /locus_tag="PKH68_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HNX13010.1"
                     /translation="KAVASLQSGEGRTYSKVIKMVKSPKTGAYTFQEEMVHNEKVKDF
                     IAK"
BASE COUNT          972 a          851 c          692 g         1201 t
ORIGIN      
        1 gaaaaacttc tgcaaaacag atatattcgc taagtagttt agcacttccg gccctatttc
       61 gttatccttg cggtgaaaaa aattccactt tcccgcaatt cgggcaaatg tacaagtcaa
      121 acgattcgcg gtttacaaac gcttccagta aatttccgaa aataccaaat ttagttcctt
      181 cgtgaaactt ataatttccc gaaagcatca ttgggacatt gcatctgagg caatttatct
      241 ttttttcagg aacggtaggt tcctcaaatt caatcacttt attttcgctg atgctgaaat
      301 tacattgcca gcacacatcg aaattatctt ccatttcagc tttacagttc ggacagtttt
      361 tcatcgttat tattgttatt tcttcattat cttcatcaca aatccgcctc cgggagccat
      421 tgaaatctgc attttccgat cagccggaat atcaactgta agttttcgat aatcgctggc
      481 tgcacggtgt gcattaaccc catcccggaa tatttctgcc ctgaaatctc cttcgcccaa
      541 aaaggataag tccagatcaa gttttcgggc atcccagttt gtcatggagc ccaggtacca
      601 gacatttcct ttacgacgtg caattgtaat gtattttgct atttccccgt tcaacgctac
      661 ggtttcatcc cacacggtag gaatttcggc aataaattca gtacattctt tttcttccat
      721 gtaagccgaa ggattgtcgc acagcatatt gagcggagac tcaaaaataa catattctgc
      781 cagctgacgg catcgtgttc cctggctcat cggttcggaa ttgacgggtc gataattatc
      841 ccgggtagca ttgcgcatgg ctccctgcgt gtaatccatg ggacctgcca ccatacggat
      901 aaagggaata gtaacatcat acgtaacctg atcgactgag gagggcgacc atttcatttg
      961 ttccagtccg tgaactcctt cgaaattaat tacattggga taggttcgtt gaaggccggc
     1021 cggcttgaat gcaccgtgaa aatcgagcat caaatgatat ttggcagcta tctgcgcagc
     1081 ccggtgataa aagtcaacca tcggctgatc gtcgcgatcc ataaagtcca ccttgaatcc
     1141 tttaattccc atctcagaat agtgtcggca gatgccttcc atgtctttat taaatgcatt
     1201 gtaacctgcc caaagaatta aatctacatt ttttgatttt gcataagcaa taagttcttt
     1261 cagatctatt tcaggaacaa tctgatacaa atcggccttg agatttaccg cccagccttc
     1321 gtccagaata acatactcaa ttttatattt cgaagcaaaa tcaatgtagt atttatacgt
     1381 ggcattattc acaccggttt gaaaatccac gtttttgata ttccagtcgt tccaccagtc
     1441 ccaggcaact tttccgggtt taatccacga gatatcggaa agctgcgatg gtgtagctaa
     1501 cttatagacc atatcactgt tggtgagttc acagtcattt tcggccacga tgacagcacg
     1561 ccagggaaaa tttgtttttc cttcacatac agcaatatag ggttcacgtg attgaaccag
     1621 gccctgcagc atgttatggc cgccgggttt aatatccctg ggaacaggtg cgaaaactcc
     1681 tgccagcccg ccggatccgt ttccgtgcaa atacataccg ggataatcca acaagtcgga
     1741 ctcggtaata cagatctttt ttccgttttt tccttcaatt accagcggta aaaatgccag
     1801 cctttttggg ttccagcttg aaagtgcaat atgttgatat tgattttcaa atgaattgaa
     1861 gaattgcttt tcgaaggtat ccttccgacc gttatcatcg tttacgtaag ctgcatagac
     1921 tttcggatct ccgggaaaat tgaattcagc ctgttcgttt tcgactgtga atggcttttt
     1981 acggtctgta ctgaaccgat aggcaactcc atcgttatag gcacgaaaaa tcaggttgta
     2041 gtcacccctg aagcttaaaa tcaactcatt ataattatct gaaattttat tttttttata
     2101 aattacggca tcaatcacct cattgaccga cttttttgta gcttttttta atttggcatt
     2161 aatgccgggt gaggttccat cggtgaacct gagagatacc ggtgattttg aaaccagcaa
     2221 atccgtctga tgaaaaaccg aatattctat cgtttttcca atattgattt cagctttcac
     2281 tttaccatcg ggtgagctga gggtaaagtt cttctgacca aagacaaaat tgcacaaagc
     2341 caaagaaacc aacaatgtca gtcctgtttt tttcatgatt gatgattttt ttttattcaa
     2401 ataaggaagc tacaaattct tctttgtcga acacctgcag gtcggtcatt ttttcaccca
     2461 gtccgatata tttcaccgga attttgaact gatcggaaat gccgataacc acaccgcctt
     2521 tggctgtgcc atcgagtttt gttacggcaa tggcattcac atcggttgcc agtgtgaatt
     2581 gttttgcctg ctcataagca ttctgtccgg tggatccatc gagtaccaac aggatttcgt
     2641 gtggtgcatc gggtgtgatc ttttgcatta catttctgat tttggtgagt tcgttcatca
     2701 ggttgatttt attgtgcaac ctgcctgccg tatcaattat caccacatcg gcattattgg
     2761 ctttggccga agcgagcgta tcgtatgcca cagaagcggg atcggcaccc attttttgct
     2821 tgacaaccgg cactcctacc ctttcgcccc agattacaag ctgctccact gctgcagccc
     2881 tgaaagtatc ggctgctccg agataaactt tctttcccgc cttcttgaat tgataagcca
     2941 gcttgcctat tgtggtggtt ttgcccactc cgtttactcc cacaaccatg ataacgtagg
     3001 gtgtcgccgg cagttcatcg tcaaagtcgg tgggttgtcc ggaattgttt tcagctaaca
     3061 atgcggcaat ttcctccttg agaattttat tcagttcgct cgtattcaaa tacttatcct
     3121 tacttacccg ttcttcaatc cgctcaatga tacgtaaggt agtttcgaca cccacatccg
     3181 atgatacgag cacttcttcg aggttgtcca gcacctcttc gtctactttt gactttccgg
     3241 caaccgctct ggtaagtttg gaaaatacac tttccttggt tttggataaa ccctggtcta
     3301 atgtttcctt tttttcctta ttaaatagtc cgaataatcc catattttta tttttttatt
     3361 tacaaattat ttacatgaaa aataccggca gttatactcc tttattgttt tgtttgacag
     3421 taaccacacc gaaatcctct aatccatttt gtaccaaagg tagatataat tttcacaaaa
     3481 ttagttattt tccactcaag taaaaaaaaa tcccttcatt ttaatgaaag gatttctctt
     3541 tatgaatatg ttgcaaaaaa cttatataat ttattttgca ataaaatctt ttactttttc
     3601 attgtgtacc atttcttcct gaaatgtata tgcaccggtt ttaggtgatt tcaccatctt
     3661 tatcaccttg gagtaggtac gaccttcacc actttgcaaa cttgcaaccg ctttct
//
LOCUS       DAOPZK010000174         3698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_33213_length_3698_cov_21.616250, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000174 DAOPZK010000000
VERSION     DAOPZK010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33213_length_3698_cov_21.616250"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PKH68_08475"
     CDS             <1..1175
                     /locus_tag="PKH68_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX13011.1"
                     /translation="TRMFLYGTIFFAITTFLCAFVPNSTLLIVMRFLQGIASAMMTST
                     LMAYVISAFPPDKRGKMIGLNVSAVYVGSSLAPMFGGLITDAFSWRSIFLINAVASSL
                     VAILILWKLNRELSETMKEKFDFKGTFLYMISISMLMYGLSKLPHQYAIILTLAGITG
                     LFVFAKIELKTKFPVLNIRLFTENRVFALSNLSALINYAATFSMSFMLSLYLQYVKGM
                     EAREAGLVLVAQPILMAIVSTFSGRLSDRKNPRKLAALGMGISAVGLLILSFISSETA
                     IIYILFGLIILGTGFGLFSSPNTNVVMGSVDKRVYGTASAILATMRSTGMMFSMAIAS
                     LTIHLFLGDAQINVSNIPQLITSTKIVFALFTVLCFIGVYTSLVKANIQIPTLKDE"
     gene            complement(1292..2275)
                     /locus_tag="PKH68_08480"
     CDS             complement(1292..2275)
                     /locus_tag="PKH68_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX13012.1"
                     /translation="MQTRKLGTGGLEVSALGLGCMGMSFGYGTVADKSEMIALIRKAV
                     ESGVTFFDTAEVYGPYTNEELVGEALAPFRNKVAIATKFGFDVGGIAGGLNSRPEHIR
                     QVVEASLKRLRVETIDLLYQHRVDPNVPMEDVAGTVKDLIQEGKVKYFGLSEAGVKNI
                     RRAHAVQPVTALQSEYSLFWREPEAEIMPVLEELGIGFVPFSPLGKGFLTGKITAETT
                     FASNDFRNTVPRFNAENRAANMALVDLLNNIAAEKEATPAQIALAWLLAQKPWIVPIP
                     GTTKPERFNENIGAVDVQLTAEDLRMIEEAASKITLHGERYQPANAKLIDR"
     gene            complement(2367..2837)
                     /locus_tag="PKH68_08485"
     CDS             complement(2367..2837)
                     /locus_tag="PKH68_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNX13013.1"
                     /translation="MKKSIFVLFLFAFVYQYSYSQQNEVKANVTGTSKAEQKIIQLSK
                     DKWQWMSDKNVDALSNLFHAKAVFVHMGGSWGTEQELNVIKTGGIWYKKADIHEVSVN
                     IIDKTAILLNRIDLLAVVGGNEVTNPFMVTEVYVKQKGKWKLGSLSFTKLITQS"
     gene            complement(2925..>3698)
                     /locus_tag="PKH68_08490"
     CDS             complement(2925..>3698)
                     /locus_tag="PKH68_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNX13014.1"
                     /translation="DAKECETSVLDAINAGYRLIDTAAAYGNEQAVGKAIKKSGVARE
                     ELFITTKLWVADVSYEKAKVAFEKSLKNLELDYLDLYLIHQPYGDVHGAWRAMEELYK
                     QGKIRAIGISNFPPDRVMDMIVFNEVVPVVNQIETHPFNQHIETQKFLIENKVQIESW
                     GPFAEGRNDIFKNELLATIGEKYNKTIAQVILRWLTQRGVVVIPKSVHKERIVENFNI
                     FDFKLSAEDMEAISTLDTGKSLFFDHRDPAIVKWMGGRK"
BASE COUNT          983 a          817 c          713 g         1185 t
ORIGIN      
        1 gaaccagaat gtttttgtac ggaaccattt tctttgccat taccaccttt ttgtgtgcat
       61 ttgttcccaa ctccacgctg ctgatagtca tgcgattttt gcagggaata gcatcagcca
      121 tgatgacaag tacgctcatg gcctacgtga tttcggcttt tccacctgac aaaagaggaa
      181 aaatgatagg actgaatgtt tcggctgttt acgtagggtc gtcactggct ccaatgtttg
      241 gtggtttaat aaccgatgct ttcagctggc ggagtatttt tcttatcaat gccgtagcaa
      301 gttcgcttgt agccattctt attctgtgga aactcaaccg cgaattatcg gaaacaatga
      361 aagagaaatt cgacttcaaa ggtacatttt tatacatgat atccatttcg atgctcatgt
      421 acggcttgtc gaaactgcct catcagtatg ctattattct caccctagcc ggaataaccg
      481 gattgtttgt ctttgccaaa atagagttaa aaacaaaatt tccggtgctg aatatccgct
      541 tatttaccga aaaccgggta tttgctctct ccaatttatc agcattgatc aactatgcgg
      601 ctacattctc catgtcgttt atgctcagcc tgtatttgca atatgtcaaa gggatggaag
      661 cgcgggaagc cggtttggta ctggtggcgc aacccattct gatggccata gtgtctactt
      721 tctccgggcg gctgtccgac aggaagaatc cccgcaaact ggcggcactg ggaatgggaa
      781 taagtgccgt gggattattg atattgagtt ttataagttc agagactgca atcatttata
      841 tactgttcgg tttaataatt ctgggaaccg gtttcggact gttttcatca cccaacacca
      901 atgtggtgat gggttcggtg gataaaagag tgtacggaac cgcatcggca attctggcaa
      961 ccatgcgcag caccggtatg atgttcagca tggctattgc ctcattgacc atccatctgt
     1021 ttcttggtga tgcgcaaata aatgtttcga atatacccca actcataacc agtactaaaa
     1081 tagtttttgc actgtttaca gttttgtgtt ttattggggt ttatacttcg ctggtaaaag
     1141 caaatattca aattccaacg ttgaaggatg agtaaagttg ataatagaag ctgactacat
     1201 aacgaagaat tattgataaa taaagccctc cggaacattc attcgaaggg ctttattatt
     1261 acttaatact gttatattat agcctaaatt attatctgtc aatcaatttt gcattggcag
     1321 gttggtatct ttctccatgg agcgtaatct tagaagctgc ttcttctatc attcgcaaat
     1381 cttcggcagt cagttgcaca tctaccgcac cgatattttc gttgaagcgt tccggtttag
     1441 tagttccagg aataggtaca atccacggtt tctgagccag cagccaggca agcgctattt
     1501 gtgcaggagt agcttctttt tcagcagcaa tattgtttag caaatctacc aatgccatat
     1561 tggctgcccg gttttcagca ttgaaacggg gaactgtatt tctgaaatcg ttgctggcaa
     1621 aagttgtttc agcagtgatt ttaccggtaa ggaaaccctt gccaagcgga ctgaaaggta
     1681 caaagccaat tcccagttct tctagcactg gcataatctc agcttccggt tcacgccaga
     1741 aaagcgaata ttcactttga agtgcggtaa caggttgcac ggcatgcgcg cgacgaatgt
     1801 tttttacacc ggcttcggac aatccaaagt attttacctt tccttcctga attaaatcct
     1861 ttacagttcc tgccacatct tccatcggaa cattcgggtc aacccggtgc tgataaagta
     1921 agtcgattgt ctccactctc aaccgtttca aagaagcttc cactacctgg cggatatgtt
     1981 cggggcgact gtttagtcca ccggctattc ctcccacatc aaaaccaaat ttggtagcga
     2041 ttgccacttt gttgcgaaaa ggagccaacg cttcacccac cagttcttcg ttggtataag
     2101 gtccgtaaac ttcggctgta tcaaaaaagg tgacaccact ttccaccgct ttgcgaatta
     2161 gcgcaatcat ttccgatttg tcggccacag tgccgtaacc gaaactcatt cccatacagc
     2221 ccagtcctaa ggcagatact tccaacccgc cggtacctaa ttttcttgtt tgcataatat
     2281 tataatttta tttcatctgt ttttttgtcc gttaatcttt caaatcaaag agacacaatg
     2341 aacctcaacc ctcccaatat caatttttac gattgtgtta tcaattttgt gaacgataaa
     2401 gaccctaatt tccatttccc tttttgtttt acgtacactt cagtaaccat aaatggattg
     2461 gttacttcat tgccacccac aacggctaac aaatcaattc tattcaaaag gatggccgtt
     2521 ttgtcaataa tgtttacgga tacttcatga atatcggctt tcttgtacca aattccgccg
     2581 gtttttatga cattgagttc ctgttcggtt ccccagcttc ctcccatgtg tacaaatacg
     2641 gctttagcat gaaagagatt gctcagggca tctacatttt tatcagacat ccactgccat
     2701 ttgtctttgg atagctgaat gattttctgc tcagctttcg aagtacctgt gacgtttgct
     2761 ttaacttcat tttgctgtga ataagaatac tgataaacga aagcgaataa gaaaagtaca
     2821 aaaattgatt ttttcatagt aactgatttt ttcattgttt ttaaaaaggg aattatttga
     2881 tctgtgaatt gtaaatggcg atccaatgaa ttatattttt acgatcattt tcttccaccc
     2941 atccacttta ctattgcagg atcgcgatgg tcaaaaaaca gactttttcc tgtatcaagc
     3001 gtggaaattg cttccatatc ttcggcgctt agtttaaagt caaagatatt gaaattctca
     3061 acaatacgtt ctttatgaac tgatttggga ataacgacaa cacctcgttg ggtaagccat
     3121 cgcaaaataa cctgtgcaat ggttttgttg tatttttctc cgatagttgc caacagttca
     3181 tttttgaaaa tatcatttct gccttcggca aaaggtcccc acgattctat ttgtacctta
     3241 ttttcaatta agaacttttg tgtctcaatg tgctgattga aaggatgtgt ttcgatttga
     3301 tttacaaccg gaacaacctc attgaatacg atcatatcca ttaccctgtc cggtgggaag
     3361 ttgctgatac cgatggctct gatttttccc tgtttgtaca attcttccat ggcacgccat
     3421 gccccatgaa catctccgta aggctgatgt atgagataca aatccagata atccagttcc
     3481 aggttcttca acgatttttc aaaagccact ttcgcttttt cataactcac atccgccacc
     3541 catagtttgg tggtgataaa gagttcttct cttgctacac cgcttttttt aatcgcttta
     3601 cccactgcct gttcatttcc ataagcagcg gcagtatcaa taagccggta accggcattg
     3661 attgcatcaa gaacactcgt ttcacattct ttggcatc
//
LOCUS       DAOPZK010000175         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_34547_length_3603_cov_21.687711, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000175 DAOPZK010000000
VERSION     DAOPZK010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34547_length_3603_cov_21.687711"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKH68_08495"
     CDS             <1..465
                     /locus_tag="PKH68_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HNX13015.1"
                     /translation="KFPVNTRGSQLDILARKAMWDLGLNYGHGTGHGVGHFLNVHEGP
                     QSIRMDENPATLQVGMITSNEPGMYRTDEYGIRTENLVLTVPAEKTEFGQFLRFETLT
                     LCFIDTRLIDKSLLNKQEEKWLNDYHKNVLKKLSPYLTKNEKKWLEKKCKEI"
     gene            complement(565..>1538)
                     /locus_tag="PKH68_08500"
     CDS             complement(565..>1538)
                     /locus_tag="PKH68_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNX13016.1"
                     /translation="LMKDNTNKALKLGVFVAVAILLFVVAVWLIGSKNNYFNPSITIH
                     TSFRDIRGLIEGNNVRFAGINVGSVKKITLVSDSAVMLDLTVSKDYAKFIYRNSVAEI
                     GQEGLVGNKLVTISSGTPESGLIENGSYLKARKSIDYDKMLQESQDILTLIRDAASDL
                     KSVAKKIDEGKGDLGKLVNENTITTGINSSISKLNTALTNIDGISKKINNGEGDLGKL
                     VNSSDITTNAQEILSGLKQTAQKADSTVGDLQKATESINRGKGTINRLLNDSITSYKI
                     DTALIKFNNTLDQFNKTARAIQDSWLIRLFPKKKTNEKSKKDTAKME"
     assembly_gap    1539..1638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1642..2370)
                     /locus_tag="PKH68_08505"
     CDS             complement(1642..2370)
                     /locus_tag="PKH68_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNX13017.1"
                     /translation="MDTVIEVKNLQKSFGNMKILRGIDLKLHKGENIGIIGKSGCGKS
                     VLTKCIVRLIEPDEGEVYISGNDVLTMNDNELTEIRKKVGYLFQGGALYDSMNIRENL
                     EFPIRRTQFIRHRPEVESMVVEALENVGLLSAINKMPAELSVGMRKRIGLARAIILKP
                     EIIFYDEPTSGLDRLTAGEISDLIVEIQEKYQASSIIITHDLKCAKTATNNIKLLKDG
                     VFYAEGTFDELSHRDDKEVRDYLT"
     gene            complement(2609..3406)
                     /locus_tag="PKH68_08510"
     CDS             complement(2609..3406)
                     /locus_tag="PKH68_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX13018.1"
                     /translation="MKNRNKISLEKPEIDYSSWFIELGALTLFTRKVLNQFFKPPFEH
                     GELIKQGYYIGYKTLPLISITGFIMGLVLTIQIRPILVDFGATSLLPGLIAVAFVREV
                     GPVITGLLCSGKISSGIAAEIGAMKVSEQIDAMEVSGTKPLGFVVATRVLATTLLVPL
                     LVIVGDAFSFLGSIVALNMDENVTFRAFMNSAFDTIHFSDLIPATIKTIFFGFFIGLI
                     GSFKGYQASQSTEAVGLAANSAVITASLTIFVIDLLAVLVTNFIVSN"
BASE COUNT          957 a          746 c          738 g         1062 t
ORIGIN      
        1 aaatttccgg tgaatacccg cggctcgcag ctggatattc tggcacgtaa agccatgtgg
       61 gatcttggac tgaattacgg acacggaacc ggtcatggag taggtcattt cctcaatgta
      121 cacgaaggtc ctcaaagtat ccgaatggat gagaacccgg ccaccctgca ggttgggatg
      181 ataacttcga atgagcccgg tatgtatcgc accgatgaat atggtatccg aactgaaaat
      241 ctggtgctga ctgttccggc agaaaaaacc gaattcggac aatttctgcg gttcgaaacg
      301 ctgacacttt gctttattga tacccggttg attgacaaga gtttgcttaa caaacaggaa
      361 gaaaagtggc tgaacgatta tcataaaaat gtacttaaaa aactttcgcc ctatctcact
      421 aagaacgaaa agaaatggct ggagaagaaa tgtaaagaga tttgaggaaa aatttcagaa
      481 cagagtagaa taataaaaat ccgaagtgtc agttgatact tcggattttt ttatttgtaa
      541 cgatttttcg atagcaggct tatcttattc catttttgcg gtgtcttttt tggatttttc
      601 atttgttttc ttctttggaa ataagcggat caaccaactg tcctgaatag cccgtgcagt
      661 tttgttgaac tgatcgagag tattattgaa tttgatgagg gcagtatcaa ttttgtaaga
      721 ggtgatgctg tcattgagca ggcggttgat ggttcctttg cctctgttta tactctcggt
      781 tgctttttgc aaatctccga ctgtactatc cgctttttgg gctgtttgtt tcaatccgct
      841 gagaatttcc tgtgcattgg tcgtgatatc gctgctgttg accaacttac cgaggtcgcc
      901 ctctccgttg ttgatttttt tactaattcc gtcgatattc gtgagagcgg tatttagttt
      961 gctaatagaa ctgtttattc cggtagtaat tgtattttcg ttcaccaatt ttcccaaatc
     1021 gcctttacct tcatcaattt ttttagcaac cgacttcaaa tcacttgctg catcgcggat
     1081 aagggtaaga atatcctgcg attcctgaag cattttgtcg taatcgatac tttttcgtgc
     1141 tttcaggtag gaaccatttt caataagtcc gctttcggga gttccggatg aaatggtgac
     1201 cagcttattg cctaccaatc cttcctgacc aatttcggcc accgagttgc ggtaaatgaa
     1261 tttggcataa tccttactga ccgtcaaatc gagcataaca gcactgtcgg aaaccagagt
     1321 gatttttttc acgctaccca cattgatgcc agcaaagcga acgttatttc cttctatcag
     1381 tccgcggata tcgcgaaatg acgtatgaat ggtgatggag ggattgaaat agttattttt
     1441 actgcctata agccagacag caaccacaaa gaggagaatg gcaaccgcta caaatactcc
     1501 taatttaagt gctttatttg tgttatcttt cataagtgnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnntt tttatgtcag gtaatccctg acttctttat cgtctctgtg
     1681 gctgagttcg tcaaaggtgc cttctgcata aaacaccccg tctttaagca gtttaatgtt
     1741 gttggtagcg gttttggcac atttcagatc gtgtgtgatg atgatggatg acgcctggta
     1801 tttttcctgt atttccacta tcaaatcact tatttcgcct gccgtcaggc ggtcgagacc
     1861 agaggtcggc tcatcataaa agataatttc gggcttcaga atgatggctc gcgccagacc
     1921 aatacgtttt cgcataccca ccgacaattc ggccggcatt ttgttgatgg cgctcagcag
     1981 acctacattt tcgagcgctt ccactaccat gctttccact tccggacgat gtctgataaa
     2041 ttgggtgcgc cgaatgggaa attccaggtt ttcgcggata ttcatcgagt cgtagagtgc
     2101 accgccctga aagagataac caaccttttt gcgaatttcg gtcagttcgt tgtcgttcat
     2161 tgtgagcaca tcattaccgg agatataaac ttcaccttca tcaggttcaa tgagccgtac
     2221 tatacacttt gtaagcaccg attttccgca tcccgatttt ccgataatcc cgatattctc
     2281 ccctttatga agtttcagat caatgccccg gagaattttc atattcccga atgatttctg
     2341 caggtttttt acttcaataa cggtatccat cggattgttt ttaaatgaac ttgtcttgac
     2401 ttattctttt aaactcagaa ataataatca aaaagaaaaa acacttgttt taaaatttat
     2461 tcaactacta attgtacgaa taacacgaat ttgttcttaa aattagtgtt acctgccaac
     2521 cgtcccgaca agtcgggata aacaacgctt gttcatagtt taatcaaacg aatattttgt
     2581 tttaataaaa gtcgagcaga cgtataaatt aatttgaaac aataaaatta gttaccaata
     2641 ccgcaagcaa atctatcaca aaaatagtaa gtgaggcggt aattacggct gaattggcag
     2701 ccagccctac cgcttcggta ctttgtgagg cctgatatcc tttgaaggag ccgataagtc
     2761 cgataaaaaa tccgaagaat atggttttga tggtagccgg tatcaggtct gaaaagtgta
     2821 tggtatcgaa ggccgagttc ataaatgctc tgaaggtaac attctcgtcc atgttgagtg
     2881 ccacaataga acccaaaaaa ctgaatgcat cgccaacgat taccaacaac ggcacaagaa
     2941 gggtggtggc gagtacccgg gtggccacta caaatccgag tggcttggta cccgaaactt
     3001 ccatagcatc aatttgttcg gagactttca tggcaccaat ttcggcagct atgcccgaac
     3061 tgatttttcc ggagcacagt aatccggtaa ttacggggcc tacttcccta acaaaagcaa
     3121 cagcaatgag acccggcagg agcgaagtgg ctccaaaatc gaccagaatg ggacgtatct
     3181 gtatggtaag caccagtccc atgataaaac cggtaatgct gatgagcggc agggttttat
     3241 aaccgatgta ataaccctgt ttgattagtt cgccgtgctc aaagggtggt ttgaaaaact
     3301 gatttaatac cttgcgggta aataaagtca gtgctcccaa ctcaataaac caggaactgt
     3361 aatcaatttc aggtttttca aggctgattt tgttccggtt tttcattaca gttgtgatta
     3421 attctgagtt tacacaatta agatcttatc tcagttccga attatgtctt gcaaccaatt
     3481 tttctctcct tcatcatccg cccttcgggc acattcgccg acttcatctg acctcacccc
     3541 cgacccctct cccttgggag aggggagaaa cgaaagcgat gtataaggtt tttcgatata
     3601 ctt
//
LOCUS       DAOPZK010000176         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_35785_length_3515_cov_25.952023, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000176 DAOPZK010000000
VERSION     DAOPZK010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35785_length_3515_cov_25.952023"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKH68_08515"
     CDS             <1..795
                     /locus_tag="PKH68_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNX13019.1"
                     /translation="VHLADFPEYDETLIDKNLEECMQLAQQISSMILALRRKAEKKVR
                     QPLTKAVIPAPDETTFKQINYIASLIKAEVNVKELEVIPSDNDMDNLVKKIKPNFKTL
                     GKKYGKQMKEIAAAMPSITKQQIAEIERNGEFTLALPTGDVLLTTEDVEIFTEDMPGW
                     LVANEGKLTVALDIVVTDELQREGVARELVNRIQNIRKANGYEITDKIQVEILRQEEI
                     NAAVEEYAEYIASQTLAASISLVDTLGEAVELDFEEYVVRINITRK"
     gene            998..1393
                     /locus_tag="PKH68_08520"
     CDS             998..1393
                     /locus_tag="PKH68_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNX13020.1"
                     /translation="MAETEKTRYSDAELEEFRTIIMEKLEKAQKDYELLRNGLSNLDG
                     NDVSDTSPTFKVLEEGASTLNKEEAGQLAQRQMKFIQHLQAALVRIENKTYGVCRETG
                     KLIPKERLRAVPHATLSIEAKGDNNKRRL"
     gene            1525..2142
                     /locus_tag="PKH68_08525"
     CDS             1525..2142
                     /locus_tag="PKH68_08525"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNX13021.1"
                     /translation="MSTSKKSLLLIILLLLIDQASKIYVKLHFQLGESVDVFPWFKIL
                     FVENNGMAFGMELFGKLFLTLFRVAAVILLAIYIRKMIKNNVRTGYVLAVSMLLAGAA
                     GNIIDSLFYGVIFNVSTPHQLAVLFPEGGGYAPFLYGKVVDMFYFPIISDRFGETIFF
                     RYIFNVADACVTVSVAIILLFFRKELNDSLESKKNVPVPTNENQD"
     gene            2126..3034
                     /locus_tag="PKH68_08530"
     CDS             2126..3034
                     /locus_tag="PKH68_08530"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNX13022.1"
                     /translation="MKIRTDLFLKILILAVVVSIFASSCQRRPSGVLNTVEMENLLFD
                     MHVMEGAMNANGADYNNPQMKEKYFQALLKKYDVTPVQFDSSVSWYTKHPKFFERIYV
                     NIDARLKKMTADVDHRKYHPIDSNATTDVNIWNLKSKYILTKDSARTKLKFDVKSNGF
                     MAGDYYLLSLLHRISRTDTTYNPHIVMYVNYWDGTTDSIYTKTHNDNLTRRYQLIFKA
                     RFDRKIKSVSGMLLGYDSVKGKMNVYLDSIRLIRRYNPVKQDSIRDMVDWLDTTVIPV
                     DTVPASIPAKLTNTTKEKKFPILTPQ"
     gene            3073..>3515
                     /locus_tag="PKH68_08535"
     CDS             3073..>3515
                     /locus_tag="PKH68_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13023.1"
                     /translation="MELLRSVVELDESGMITGVISLDNTSVEPAHTLFLDGIITPGIV
                     SIREFTKKGEKTPDLTNYHYLDLSDGEVLPEVINTDLPLIIDFGTSNLSQANKIIEKF
                     ASSGLSIRQLVDACIFHPAEALGICVDVNVGNKTNLIRWQNVDVL"
BASE COUNT         1073 a          781 c          760 g          901 t
ORIGIN      
        1 gttcacctgg ccgatttccc ggaatacgat gaaacactga ttgataaaaa tctggaggaa
       61 tgtatgcaac tggcacagca gatctcgtcc atgatactgg ctctgcgccg caaggccgaa
      121 aagaaagtgc gtcagccgct gaccaaagct gtaattcccg ctcccgatga aacgaccttt
      181 aaacagataa attacatcgc ctcactgatt aaagccgaag tcaatgttaa ggaactggaa
      241 gtcatcccat cggataacga tatggataat ctggtgaaga agataaaacc caatttcaaa
      301 acactgggaa aaaaatacgg caaacagatg aaggaaatag ctgccgccat gccttcgatt
      361 accaaacagc aaatagccga aatagaacga aacggtgaat ttacacttgc attgcccaca
      421 ggtgatgtac tgctcaccac cgaagatgtg gaaatattca ccgaagatat gccgggatgg
      481 ctggtggcca atgaaggaaa actgaccgta gcactcgata ttgtagtcac tgacgaattg
      541 cagcgcgaag gggtagcccg tgaactggtc aaccgtattc agaatatacg caaggctaac
      601 ggttacgaga tcaccgacaa aattcaggtg gaaattctcc gtcaggagga aattaatgcc
      661 gccgtggagg aatacgccga atatatagca tcacagacac tggcagcttc catcagtctg
      721 gtggatacgc tgggtgaagc ggtggaactc gacttcgaag aatatgtggt aagaataaac
      781 attacccgta aataattccg gcgatacgac agttcatcat taaaataaag aattgcaccg
      841 gtatgcttgt tattgctgaa tttgagttat atttgcagcc tcgttgctca actattttta
      901 attaaaaata attggttttc tgaagaataa ttcttacttt cgcaaactta aatttccggg
      961 atattgaaat aatatctaaa aaaaaacacc gataattatg gcagaaacag aaaaaacaag
     1021 atattcggat gcagaactcg aagagttccg gactatcatc atggaaaagt tggaaaaagc
     1081 gcaaaaagat tatgaactgt tgcgtaatgg actgagtaac ctcgatggca atgatgtatc
     1141 cgatacttca cccaccttca aggtgctcga agaaggtgct tcaactctta acaaagaaga
     1201 agcaggtcag ctggcacaac gacagatgaa atttatacag catttgcagg ctgcattggt
     1261 tcgtatcgaa aacaaaacct atggtgtatg ccgcgaaaca ggtaaactga tacccaagga
     1321 gcgtctgcgt gccgtacctc atgctacgct gagtatcgaa gcaaaaggcg acaataacaa
     1381 aagaagactg taaaattgtc aacgaataaa ctcactgaga atacctgatt acaaacttta
     1441 attcggcaac ccggcttacg ttggttgtcg aatttttgta tgaaacgaac attccgcaaa
     1501 tcggaaaata aattcaatca acacatgtca acgtccaaaa aatcgctttt gctcatcatc
     1561 ctgcttcttc ttattgatca ggcatccaaa atatacgtta aactgcattt ccaactggga
     1621 gagtcggtcg atgtatttcc ctggttcaaa atcctgtttg tggaaaacaa cggaatggca
     1681 ttcggtatgg aactcttcgg gaaattattc ctgactctct ttcgggtggc ggctgtaatt
     1741 ttgctggcaa tttatatccg taaaatgata aaaaacaatg taagaaccgg ttatgtgctc
     1801 gccgtatcca tgctgctggc cggagcagcc ggcaatatca tcgattcgct cttctacggc
     1861 gttattttca atgtcagcac acctcatcag ctggcggtac tctttcccga aggaggcggc
     1921 tatgctccct ttttgtatgg caaagtggtg gatatgtttt attttccaat catctccgac
     1981 aggtttggcg aaaccatatt cttccggtat atatttaatg tggctgatgc ctgtgtgacc
     2041 gtttcggttg ccattatcct gctttttttc cgcaaggaac tgaacgatag tctcgaatcc
     2101 aaaaaaaatg tacctgtacc caccaatgaa aatcaggact gatctttttc tgaaaatatt
     2161 aattctggca gtggtagtca gcatttttgc atcatcctgc cagcgtcgcc cttccggtgt
     2221 actcaatacc gtggagatgg aaaatctcct gttcgacatg cacgtgatgg aaggtgccat
     2281 gaatgctaac ggggctgatt acaacaatcc tcagatgaaa gagaagtact ttcaggcatt
     2341 actcaagaag tacgacgtta ctccggtcca gttcgattcc tccgtttcgt ggtacaccaa
     2401 gcatccaaag ttttttgagc gaatctatgt caatatcgat gcccggctca agaaaatgac
     2461 cgccgatgtg gaccaccgga aataccatcc catcgattcg aatgccacta ctgacgtgaa
     2521 tatctggaat ctcaagtcaa agtatattct gacaaaggat tctgcccgta cgaagcttaa
     2581 atttgatgtg aaaagtaacg gattcatggc aggcgattat tacctgcttt cgctgttgca
     2641 ccgcatatcg cgcaccgata ccacctacaa tccgcacata gtgatgtatg tcaattactg
     2701 ggacggcacc accgacagca tatacaccaa aacccacaac gacaacctca cccgtcgcta
     2761 tcaactgata tttaaggcac gcttcgaccg caagatcaaa tcggtgagcg gtatgctcct
     2821 gggctatgat tcggtgaagg gaaaaatgaa tgtgtatctc gacagtatcc ggcttatccg
     2881 ccgctataat ccggttaagc aggacagcat ccgcgatatg gtggactggc tggataccac
     2941 ggtaatacct gtagatactg ttccggcttc tatccctgca aaattgacaa atacaacgaa
     3001 agaaaagaaa tttccgatac tcacacctca gtgaaacgct tcacggcaca tctaatattc
     3061 ccatcagcag gggtggaact tctgcgctct gtggtggaac tggatgagag cggtatgatt
     3121 accggtgtta tttcattgga caatacatcg gttgagcctg cccacacact atttctggac
     3181 ggaataatta cccccggaat tgtatccatc agagaattta ccaaaaaagg tgaaaaaact
     3241 cccgatctga cgaactatca ctatcttgat ttatcggatg gtgaagttct gcccgaagta
     3301 atcaacaccg atttgcctct aatcatcgac ttcggaactt caaatctttc ccaggctaat
     3361 aaaataatcg aaaaatttgc atcttccggg ctttcaatcc gccaactggt ggatgcctgc
     3421 atttttcatc ccgctgaagc gttgggaata tgtgttgatg taaacgttgg gaataaaaca
     3481 aatctaatcc ggtggcaaaa tgtggatgtg ttgaa
//
LOCUS       DAOPZK010000177         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_36792_length_3451_cov_22.736160, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000177 DAOPZK010000000
VERSION     DAOPZK010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36792_length_3451_cov_22.736160"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PKH68_08540"
     CDS             <1..1482
                     /locus_tag="PKH68_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNX13024.1"
                     /translation="LVVSVLLFFSLLVGKTGHKYGVPTLLLFLLIGILAGTDGLGLEF
                     ESPKVTQFIGVIALNIILFSGGMDTDLREVKPVVAQGLVLATVGVLLTALLTGLFIFW
                     ITNHVFTSVSFTLLESLLLGSIMSSTDSASVFSILRSKGLSLKENIRPLLEFESGSND
                     PMAFMLTVVLIQLIQTPDFSSGEAIFMFFKQLVLGAAGGLFLGKLSVRIINKINLDND
                     ALYSVLLFTIMFFLFGFTSFVGGNGYLAVYAGGLLIGNHKFVHKRSSLKFFDGLTWLF
                     QIIMFLTLGLLVNPKELLPIAGVALLIGFFMTLISRPAAVFIGLSPFRKMSFRARSYV
                     SWVGLRGAVPIIFATYPWIENVPKAKMIFNIVFFITILSLVIQGTTVGTLAHWLKLSL
                     PVTKKRKLREFDVEFSDEIKSAMSEISIKEEFLANGSRLMDINVPDKTLVAMVKRKGR
                     FFIPRGNTELLVGDRILVITDDEKALEETYQSMKGKVPEVKPK"
     gene            1516..>3451
                     /locus_tag="PKH68_08545"
     CDS             1516..>3451
                     /locus_tag="PKH68_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNX13025.1"
                     /translation="MKKSGIKNLTSISGFAFALLIGTFGSLHAQNETKTTSLTSKSSV
                     RIIDAKKINPTTVEIEFSNQQKMLVDFYGNNIFRLFQDNSGKGLRDPQAQPEAKILLD
                     NPRKQLAKLDVTDRNNNLTLSTNAVQVDFDKNTTCFTIKNLLTNREVVKSTTPVIFDK
                     SKVSITLSESADEYFYGGGVQNGRFSHKGKAVAIENQNSWTDGGVASPTPFYWSTNGY
                     GFMWHTFNKGKYDFGLKEKNTVTLSHDTDYLDVFFMVGDGAVALLNDFYQLTGKPVLL
                     PKFGFYEGHLNAYNRDYWKEDPKGTLFEDGKRYKESQKDNGGIKESLNGEKNNYQFSA
                     RAVIDRYKQHDMPLGWILPNDGYGAGYGQTSTLEGNIQNLKEFGNYARNNGVQIGLWT
                     QSDLHPKEGVEALLQRDILNEMVRAGVCVLKTDVAWVGAGYSFGLNGIQDVANIMSKY
                     GITTRPFIISLDGWAGTQHYATIWSGDQTGGVWEYIRFHIPTYIGSGLSGQPNITSDM
                     DGIFGGNNKAVNIRDFQWKTFTPMQLNMDGWGSNEKYPHALGEPAASINRNYLKLKSE
                     LLPYAYSIAREAVDGLPMIRAMFLEEQNPYTLGKQTQYQFMYGPNFLIAPVYQNTKSD
                     TLGNDVRNHIYLPEGSWVDYF"
BASE COUNT          964 a          773 c          780 g          934 t
ORIGIN      
        1 cttgttgtat ctgtcctgct tttttttagt ttactggtcg gtaagacagg acacaaatac
       61 ggtgttccga cgttgttatt attcctctta atcggtatcc tggcaggcac cgacggtctc
      121 ggactggagt tcgaatctcc caaagttacc cagtttattg gcgtaatagc tctgaatatc
      181 attctgtttt cgggcggaat ggataccgac ctgcgcgaag tgaaaccggt ggtagcacag
      241 gggctggtgc tggccacggt gggtgttttg cttactgcac tccttaccgg actgtttatt
      301 ttctggatta caaaccatgt gtttacgtca gtttccttta ccctgctcga gtcgctgttg
      361 ttgggaagca tcatgtcgtc caccgattcg gcttccgttt tctccatttt acgttccaaa
      421 ggactttcgc tcaaagaaaa tattcgcccg ctgctggagt ttgagagcgg cagcaacgat
      481 cctatggcat ttatgctcac ggtcgttctt attcaactta ttcaaacacc cgatttcagt
      541 agcggtgaag ccatattcat gtttttcaaa cagttggtgt tgggtgctgc cggtggactt
      601 tttctgggta aattatccgt acggataatt aataaaatta acctggacaa tgatgccctc
      661 tactccgtgt tgctttttac catcatgttt ttccttttcg gatttaccag ttttgtaggt
      721 ggtaatggtt atctggcagt gtatgcgggc ggtttgctca ttggcaatca caaatttgta
      781 cacaagcgca gttcactcaa gtttttcgac ggactgacct ggctctttca aatcatcatg
      841 ttccttaccc tggggttatt ggtcaatccc aaggaactgc tccccattgc gggtgtagcc
      901 ttgttaatcg gctttttcat gaccttgata tcccgcccgg cagccgtatt tatcggcctc
      961 tctccgttcc gcaaaatgtc attccgggcc cggtcatatg tgtcgtgggt gggattgaga
     1021 ggagcagtgc ctatcatttt tgccacttat ccgtggatag aaaatgtgcc taaggccaaa
     1081 atgatattca atatcgtatt tttcatcacc attctttcgc tggttattca gggtacaaca
     1141 gtcggcaccc tggctcactg gctgaaacta tcacttcccg taacaaaaaa acgtaagttg
     1201 agagagtttg atgttgaatt ctcggacgaa ataaaatcgg caatgagcga aatatccatc
     1261 aaggaagaat tcctggccaa cggctcccgt ctgatggata tcaatgtgcc cgacaaaacg
     1321 ctggtggcaa tggtcaagcg gaaaggaaga ttttttattc cccgtggaaa taccgaactc
     1381 ctcgttggtg accggatact ggtaatcacc gatgacgaaa aagccctgga ggaaacctac
     1441 cagtcgatga aaggaaaagt cccggaagta aagcccaaat aacctcaaat caaaataaat
     1501 ctatacaaaa taaaaatgaa aaaatcagga attaaaaatc ttacatcaat ttcaggcttt
     1561 gcatttgcgt tgcttattgg aacttttggc agcctgcatg ctcagaatga aaccaaaacc
     1621 acgtcactta cttctaagag ttcagtacgg attattgacg ccaaaaaaat taatcctact
     1681 acggtggaaa ttgagttttc caatcagcag aagatgttgg ttgattttta tggaaataat
     1741 attttccgcc tttttcagga taattccgga aaaggattaa gagatccgca ggctcagccg
     1801 gaagcaaaaa tcctgctcga caatccccga aaacagcttg ctaaacttga tgttactgac
     1861 agaaacaata atctcaccct tagcaccaat gctgtgcagg ttgattttga taaaaacacc
     1921 acttgcttca ctatcaaaaa tttgcttacc aaccgggaag tggtcaaatc aacgacaccg
     1981 gtaatatttg ataagagcaa agtttccatc acactgagcg aaagtgccga tgagtacttc
     2041 tatggcggtg gtgtgcaaaa cggacgtttt tcacacaaag gaaaagccgt tgccatagaa
     2101 aaccaaaaca gctggaccga tggcggtgta gcttcaccca ctcctttcta ctggtcaacc
     2161 aatggctatg gttttatgtg gcacaccttc aataaaggga aatatgactt cggattaaaa
     2221 gaaaaaaaca cggtaacact ttcgcacgac accgattacc tggatgtatt ctttatggtc
     2281 ggtgacgggg ctgttgcctt gttgaatgat ttctaccaac tcaccggaaa accggtattg
     2341 ctgcctaaat tcggtttcta cgaaggacat ttgaatgcct ataaccgcga ctactggaaa
     2401 gaagatccga aaggaacgct ttttgaagac ggtaaacgtt acaaagagtc gcagaaagac
     2461 aacggcggga taaaagaatc gctcaacggt gagaagaaca actaccaatt ctctgcccgc
     2521 gctgtaatcg accgttacaa acaacacgac atgccgctgg gttggatttt acctaacgac
     2581 ggatacggag ccggttacgg acaaacatct acgttggaag ggaacatcca aaacctgaaa
     2641 gagtttggta actatgcccg caacaacgga gtacaaattg gtctctggac acagtccgac
     2701 ctgcacccga aagaaggagt tgaagcactg ttgcagagag atattctaaa tgaaatggtc
     2761 agggccggtg tatgtgtgct caaaacagat gttgcctggg tgggagccgg ttattcattc
     2821 ggactgaacg gaatacagga tgtcgcaaac atcatgtcta agtacggtat taccacacga
     2881 ccatttatca tttcactcga cggctgggca ggtacacagc attatgccac tatctggtcg
     2941 ggcgaccaga ccggcggtgt gtgggaatac atccgttttc acattcccac atatattggt
     3001 tcgggactct ccggacaacc aaacattacg tccgatatgg atggaatttt cggaggaaat
     3061 aataaagcgg tcaatatccg tgatttccag tggaaaacat ttactcccat gcagttgaac
     3121 atggacggct ggggaagcaa cgaaaaatat ccacacgcac tgggcgaacc ggcggcgtct
     3181 atcaaccgga attatctcaa actgaaatca gaacttttac cttatgcgta cagcattgcc
     3241 cgcgaagcgg ttgacggact gcccatgatt cgcgctatgt ttttggaaga acaaaatcct
     3301 tatacgttgg gcaaacaaac ccagtatcag tttatgtatg gacctaactt cctgattgca
     3361 cctgtttatc agaatacaaa atcggataca ctcggcaacg atgtacgtaa tcatatttac
     3421 ctgcctgagg gtagctgggt ggattatttc a
//
LOCUS       DAOPZK010000178         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_37523_length_3405_cov_26.853731, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000178 DAOPZK010000000
VERSION     DAOPZK010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37523_length_3405_cov_26.853731"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKH68_08550"
     CDS             <1..666
                     /locus_tag="PKH68_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13026.1"
                     /translation="LTATPIPNFTDLCYADVTQEAIHNFSNGNKNLFYISGKVSYNIP
                     DINFSTEDYQTSMELIEDSVIQVNCSFTNKIRVYNGPGLPATIQDTRITQRIEYDFIT
                     GKTMIHQFTKTVNKWGAYHDNFTDTDKPEYTFEIEEKDQKMRLRKILNLVPDNQSWNG
                     ITLLFETKNTEETRNTVEELEETTKTTQYDESGKETGSTTVNYINTDYTSDNTMRIWF
                     HNK"
     gene            complement(1059..1805)
                     /locus_tag="PKH68_08555"
     CDS             complement(1059..1805)
                     /locus_tag="PKH68_08555"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNX13027.1"
                     /translation="MKKSLFLLLIILLSVSCAPRRWVMTGYKTSKIAIDATTDSLADK
                     KMQAYILPVKQNLDKEMDQIIGTSDEVMKAGKPESLLSNWNSDVYRIAAAEYLKEPVD
                     MAVVNMGSLRSPLPAGNIRVRDIFQLMPFENELVILWLKGSDVIKLMEIFAKEGGQGV
                     SGISMEIKEDKPENILIGGQPINPEKLYSIATNDYLAGGNDRMVPLATPAKRVDTGLK
                     IRNILMEYVIRENRNGRHIGSTLDGRITIR"
     gene            1947..3239
                     /gene="tyrS"
                     /locus_tag="PKH68_08560"
     CDS             1947..3239
                     /gene="tyrS"
                     /locus_tag="PKH68_08560"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNX13028.1"
                     /translation="MNFIEELAWRGMIHSMMPGTEEQLQKEMTSAYVGIDPTADSLHI
                     GHLVSVMMLRHFQRAGHKPIALVGGATGMIGDPSGKSAERNLLDEAALRHNQECIKAQ
                     LAKFLDFESNSPNAAELVNNYDWMKDYSFLDFIRDIGKHITVNYMMAKDSVKKRLSSE
                     SSEGMSFTEFSYQLLQGYDFLNLYQTKNCKLQMGGSDQWGNITTGTELIRRKTGGEAF
                     ALVCPLITKADGGKFGKTESGNVWLDRRYTSPYKFYQFWLNVSDADAEKYIKIFTAIS
                     KEEIESLTEEHRQAPHLRALQKRLAREVTVLVHSQEDYDAAVEASNILFGNATSDVLK
                     KLDEETLLAVFEGVPQFKISLTELESGVKAIELLTDIAPVFPSKGEMRKLVQSGGVSL
                     NKEKLTDQEQHISRTDLLNDKYLLVQKGKKNYYLLIAE"
     gene            complement(3226..>3405)
                     /locus_tag="PKH68_08565"
     CDS             complement(3226..>3405)
                     /locus_tag="PKH68_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13029.1"
                     /translation="VVSPGLEPGQAEPKTAVLPLHHETIIILLSLKSGAKIRLFFVFQ
                     NNLSKMYLCRGIIPL"
BASE COUNT         1059 a          756 c          675 g          915 t
ORIGIN      
        1 cttaccgcta cccctattcc taacttcacc gatctgtgtt acgccgatgt aacccaggaa
       61 gccattcata acttcagcaa tggaaataaa aacctgttct atatttctgg aaaagttagc
      121 tataatattc cggatattaa tttcagtacg gaagactacc agacttcaat ggaattgatt
      181 gaggactctg ttattcaggt taactgcagc tttaccaaca agatacgagt ttacaacgga
      241 ccaggtttac ccgctacaat tcaggacaca cgcattacac aacgcataga atacgacttt
      301 attaccggaa aaacaatgat acaccagttc acaaaaaccg taaataaatg gggtgcttat
      361 cacgacaact ttactgatac cgataaaccg gaatatacct tcgaaataga agagaaggat
      421 caaaaaatga ggctcaggaa aattctcaat ctggttcctg ataatcaatc ctggaatggt
      481 atcacgctgc ttttcgaaac aaaaaacacg gaggaaacca ggaatacagt ggaagaactc
      541 gaagaaacca ctaaaaccac acaatacgac gaaagcggaa aggaaacagg tagcactacc
      601 gtaaattata tcaataccga ttatacatca gataatacaa tgcgtatctg gtttcacaac
      661 aaataataaa aataaaaaat attcggattt cagttttttc ggtttattgc tttcagccgc
      721 aaatccatga acttgtgatc cgaatattta accggataaa ttcgctaaaa acaactatcg
      781 ttaagctcat gtttcacttt cactacattt tcacagaaca gaaaaagatg aaatataaaa
      841 gtttttacga tttgcccgat tatttatacg atttgcgcag tccttttaat attcaataag
      901 tatttttgct tcacaaatta aaagttgcaa aaaaaatttg gaaagaacta ttacaatttt
      961 caaacaaact aaacctaaat ttctgttgtt tacaccacaa aaagagagtt acctatatcc
     1021 cgggtaactc tctgattttt ttatttaatc cgcttatctc agcgaattgt aattctcccg
     1081 tccaacgttg aaccaatatg gcgcccgtta cgattttcgc ggatcacgta ttccatcagt
     1141 atatttctta ttttcagacc ggtatccact cttttcgccg gagtggcaag cggcaccata
     1201 cggtcgttgc caccggcaag gtagtcattg gtagcaatac tataaagttt ttcaggatta
     1261 atgggctgtc cgcctatcag gatattttca ggtttatctt cttttatttc catactaatt
     1321 cctgacactc cctggccgcc ttctttagca aaaatttcca tcagttttat gacgtcagac
     1381 cctttcagcc agagtatcac cagttcgttt tcgaaaggca tcaactgaaa gatatcgcgt
     1441 acacgtatat tgcctgcagg gagcggagag cgaagacttc ccatattcac cacagccata
     1501 tccacgggtt ctttcaaata ttcggcagcc gctatccggt acacatccga attccagttg
     1561 gacaacagac tttcgggctt tccggctttc attacctcgt ccgacgtacc gataatctga
     1621 tccatttctt tatcaaggtt ttgttttacc ggcagaatat atgcctgcat ctttttatca
     1681 gccagcgaat cggtagtggc atcaatggca attttcgaag ttttgtaacc cgtcatcacc
     1741 caccggcgag gagcacagga tactgacagc agaatgatca gaagaagaaa cagggatttt
     1801 ttcatacaat tgatgttgtg agagtaaatg taggattatt ttacaaatat aattaagttt
     1861 ttttgttctg ttccaatcta acccctattt ttcacttaat gaaaaaatgg ctatctttgc
     1921 gctccattaa ttaatatttt attctgatga attttatcga agaacttgcg tggcgcggca
     1981 tgatacacag catgatgccc ggcaccgaag aacaactcca gaaagaaatg acttcggctt
     2041 acgtgggcat tgaccccacc gccgactcac tccatattgg tcacctggta agcgtgatga
     2101 tgcttcggca ctttcagcgt gccggtcaca aacccatcgc actggtgggt ggagctaccg
     2161 gcatgattgg cgatccgtcg ggcaaatcag ctgagcgtaa tctgcttgat gaggctgctc
     2221 ttcgccacaa tcaggaatgt atcaaggcgc aattagccaa atttctcgac tttgaaagta
     2281 attcgcccaa tgctgccgag ttggtcaaca attacgactg gatgaaggat tattcattcc
     2341 ttgattttat ccgcgacatt ggcaaacaca tcaccgtaaa ctacatgatg gccaaggact
     2401 cggtaaaaaa acggttgagc agtgagtcaa gcgaaggtat gtcgtttact gaattttcat
     2461 atcaactcct gcagggttac gattttctga acctctatca gactaaaaac tgcaaactgc
     2521 agatgggtgg ttcggatcag tggggcaaca tcacaacagg tacagaactc atccgccgaa
     2581 aaaccggagg agaggcattt gcactggttt gtccgctgat aaccaaagcc gacggaggaa
     2641 aattcggtaa aaccgaaagc ggcaacgtat ggctcgaccg acgctataca tctccctaca
     2701 aattctatca gttctggttg aatgtaagcg atgccgatgc cgaaaaatac atcaagatat
     2761 tcaccgccat tagcaaagaa gaaattgaat cgttgaccga agagcaccgg caggcacccc
     2821 atctgcgagc cctgcagaaa cgccttgccc gtgaagtaac cgtactggta cattcgcagg
     2881 aagactacga tgcagcagta gaagcatcaa atattttatt cggcaatgcc acctccgatg
     2941 tcctcaaaaa gctggatgaa gagaccctgc tggctgtatt tgaaggagtt cctcagttta
     3001 aaatttcact cacagaactg gaatccggag ttaaagccat cgaactgctg accgatatag
     3061 caccggtatt cccctcgaaa ggagaaatgc gaaagctggt acagagcggt ggtgtaagcc
     3121 tgaacaagga aaaactcact gatcaggaac aacatatcag tcggaccgac ttactcaatg
     3181 acaaatacct gctggtacag aagggaaaaa agaactatta tctgcttata gcggaataat
     3241 acctctgcaa agatacattt tcgataaatt attttggaag acaaaaaaaa gtcgtatctt
     3301 tgcaccgctt ttgagagaaa gcaaaatgat gattgtctca tggtgtaatg gtagcactgc
     3361 agtttttggt tctgcctgac caggttcgag tcctggtgag acaac
//
LOCUS       DAOPZK010000179         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_37779_length_3388_cov_25.249025, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000179 DAOPZK010000000
VERSION     DAOPZK010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37779_length_3388_cov_25.249025"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..1208)
                     /gene="prmC"
                     /locus_tag="PKH68_08570"
     CDS             complement(330..1208)
                     /gene="prmC"
                     /locus_tag="PKH68_08570"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNX13030.1"
                     /translation="MHHFLKILKAELKNSYSDSEIRTFGMLILEKITGFSRTQLLVNK
                     EFRLNNEQNQLASQYLERLKNHEPIQYILGETEFCGLKFKVNPGVLIPRPETEELVEW
                     ILTDLPPNPLKGEENPPFGGWGGFLDIGTGSGCIAVSLKKKFPSATVSAMDISAEALE
                     VASQNTALNQVDVEFIQDDILNPVPTDRKWEVIVSNPPYIPASEHSEMDRNVTDYEPH
                     LALFVSNDDPLIFYRKIAGFALSHLTTGGHLYFEIHQNLAYECKQLLEEIGFTNVEIR
                     RDLSGNERMVRGELPG"
     gene            1331..2428
                     /locus_tag="PKH68_08575"
     CDS             1331..2428
                     /locus_tag="PKH68_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNX13031.1"
                     /translation="MSKYIYQKENWTDFVWDNAIVSAVLGDVRLLQGKMLGQIHSLGF
                     SSKEEKKLEMLTLDVLKSSEIEGETLNYEQVRSSVARRLGINTAGLVTSPRDVEGVVE
                     MMLDATQNYSEPLTEDRLFGWHAALFPTGYSGMHKITVARYRTEEMQIVSGAMGKEKV
                     HYEAVAATDVEKEMKLFLDWFNDDTIQIDPVLKAAIAHFWFIIIHPFDDGNGRIARAI
                     SDRMLARSESSKERFYSLSKQILAERNDYYAVLKKTQHNNDITDWLVWFLNCLKTALL
                     ETENSMQNILLKTEFWEKHKDIQINERQRLMINKLFDDFYGKLTTSKWAKITKTSTDT
                     ALRDIKDLMEKGILQQEEAGGRSVNYSLIKL"
     gene            2620..>3388
                     /locus_tag="PKH68_08580"
     CDS             2620..>3388
                     /locus_tag="PKH68_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNX13032.1"
                     /translation="MRRFTAFLLFISFVISLSAQQYPKREMRAVWIATVANIDWPSQS
                     GLTPTQQRIEMRNMLDGLQACNINTIVLQIRPTADSFYPSDLEPWSNYLSGKQGQRPN
                     PYYDPLQFIIEEAHKRCMEVHGWVNPYRVTNTAGQINRLDKSHVYYKNRDLIVKYGEK
                     YYFNPGLDETREFLNKVVEDIVGHYDIDGLHFDDYFYPYPEGSKDFPDEETFRANPRG
                     FTNKADWRRNNVNMVIEELQKTIKDMKPWVQFGVSPFG"
BASE COUNT         1031 a          698 c          745 g          914 t
ORIGIN      
        1 ccccttcaga gcttgtcccg gatatatttc gggagggttg ggggcagaaa agtatttctt
       61 ctgtacactt tacggataat cattaatggt taatagtgat ttttctgaaa aaaaaatata
      121 tatcagtatt tatggaatat gaaatttgaa aaaactgcct tatttacaac tacttaaaaa
      181 atttgtcatt aaaaaacaaa tataagacaa caaatccacc ggtgcaatgc acgaaaaaaa
      241 aaatcatgta aattttatgc tgatacaggt taaactctaa accaacaatg gcaaggattt
      301 gaatgaaaaa ttttccggca ggaataaatc taccccggca gctcccctct caccatccgc
      361 tcatttcccg acaagtccct tcgaatttcc acgttggtaa atccgatttc ttcgaggagc
      421 tgtttgcatt cataagccag gttttggtgt atttcgaaat aaaggtgacc gccggtagtg
      481 aggtgagata aagcaaatcc ggcaattttg cgatagaaaa taagcgggtc atcgttgctg
      541 acaaacagcg ccaggtgcgg ctcgtagtcg gttacatttc ggtccatttc actgtgttcg
      601 gatgcgggaa tgtagggtgg attgctgaca atgacctccc actttctgtc tgttggtacg
      661 ggattgagaa tgtcatcctg aataaattca acatccacct gattgagagc ggtattttgg
      721 ctggccactt ccagtgcttc agccgagata tccatggccg aaaccgttgc tgatggaaat
      781 ttctttttta atgaaacagc aatgcatcca ctgccggtgc caatatccag gaagcccccc
      841 caccccccaa aggggggatt ttcttccccc tttagggggt tagggggcag gtcagttaaa
      901 atccactcca ccagttcttc cgtttccggt cgcggtatca acacgccggg attgactttg
      961 aatttcagtc cgcaaaactc cgtttcaccc agtatatatt gaatgggttc gtggtttttc
     1021 aatcgctcaa ggtattggct ggcaagttga ttttgctcat tatttaacct gaattcttta
     1081 tttaccaaca gttgggtacg cgaaaagccg gttatttttt cgagtatcag catgccgaac
     1141 gtgcgaattt cactatcgga atagctgttt ttaagttcgg cctttaatat tttcaggaag
     1201 tgatgcatga tgcaaaagta gtaaaattca gtaaaacaca tcacagcaaa tgatatgcgg
     1261 tgaaaaaagt tgttatttac cgcaaatatg cggtgaataa gttgtatatt tgccgcaaaa
     1321 gaattaaacc atgtcaaaat acatttatca aaaagaaaac tggaccgatt ttgtgtggga
     1381 taacgctatt gtaagtgccg tattaggtga tgtccgtctt ttgcaaggca aaatgttggg
     1441 acagatccat tcccttggtt tttcgtcaaa agaagaaaag aagctggaga tgcttacgct
     1501 ggatgtgctg aaatcttcag aaatagaggg cgaaacgtta aattacgaac aggttcgctc
     1561 ttccgttgcc cgaaggctgg gcataaacac agcagggtta gttacttcac cccgcgatgt
     1621 ggaaggcgta gtggaaatga tgctggacgc aactcaaaat tattcagaac ccctcacaga
     1681 agatcgtcta ttcgggtggc atgcagcgct tttccctacc ggatatagcg gtatgcacaa
     1741 aattacagtg gctcgctatc gaacggaaga aatgcagatt gtttcgggtg cgatgggtaa
     1801 agaaaaagtg cactacgaag ctgtggcggc aacggatgta gaaaaggaaa tgaagctgtt
     1861 tttggattgg tttaatgacg acaccattca gattgatccg gtattaaaag ccgctattgc
     1921 ccatttttgg tttataatta ttcatccctt cgacgacgga aacggacgaa ttgcgcgggc
     1981 tatttcagac agaatgctag cccgaagcga aagcagtaag gagcgttttt acagtctttc
     2041 aaaacaaatt ctggcggagc gaaacgatta ttacgctgtt cttaaaaaaa cgcaacacaa
     2101 caacgatatt acggattggc ttgtttggtt tctcaactgt ctgaaaacag ctttactgga
     2161 aaccgaaaat tccatgcaga atattttact taaaactgaa ttctgggaaa agcataaaga
     2221 tatacagatc aacgagcgtc aacgactgat gatcaataag ttatttgatg atttttacgg
     2281 gaaattaaca acatcaaagt gggcaaaaat cactaaaaca tctaccgaca ctgcattacg
     2341 cgacattaaa gatttaatgg aaaaaggcat attacagcaa gaagaagccg gaggacgaag
     2401 cgtaaattat tctttaatta agttgtgata ccggatgtgt cgattgcaaa actatcggga
     2461 gcatcaaatc cgtttgtttt ttcaatgaac gcaattggac ttagcacact cttgctgtga
     2521 caagaaaaat gattttacaa tgcctaagat atattcaact tgcacagcgc agaaaaatag
     2581 cttatttttg ttcaaaaatt tataaacttc gtatttgtca tgagaagatt cactgccttt
     2641 ttgctcttta tttcgtttgt catcagtctt tcagcccaac aatacccgaa acgcgaaatg
     2701 cgtgccgtgt ggatagccac ggtagccaat atcgactggc caagccagag cggacttacg
     2761 cctacccagc aacggattga aatgcgcaac atgctggacg gcctgcaagc ctgcaacatt
     2821 aacaccatag tactccaaat acgtcctaca gccgatagtt tttatccttc ggacctggag
     2881 ccgtggtcca attacctgag cgggaagcag ggtcagcgtc caaatccgta ctacgatccg
     2941 cttcagttca tcattgaaga agctcacaaa cgctgcatgg aagttcacgg atgggtcaat
     3001 ccgtaccgcg taaccaatac cgccggccag attaaccgac ttgacaaaag ccacgtttac
     3061 tataaaaaca gagatttgat agtaaaatac ggggagaaat actacttcaa tccgggatta
     3121 gacgaaaccc gtgagtttct gaacaaagtg gtggaagaca tcgtaggtca ttatgacatt
     3181 gacggtctgc acttcgacga ttacttctac ccctatcccg aaggcagtaa agattttccg
     3241 gatgaagaga ctttccgggc taatccgcgc ggattcacca acaaagccga ctggcgcaga
     3301 aataacgtaa atatggttat tgaggaactt caaaagacca tcaaagacat gaaaccctgg
     3361 gttcagtttg gtgtaagtcc gtttggtg
//
LOCUS       DAOPZK010000180         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_38315_length_3355_cov_20.478182, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000180 DAOPZK010000000
VERSION     DAOPZK010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38315_length_3355_cov_20.478182"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PKH68_08585"
     CDS             <1..1004
                     /locus_tag="PKH68_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX13033.1"
                     /translation="KKVVSDYPGSEEADVALESLETIYVEQNEVDDYLNYTRSLGRNT
                     GDNNSAREDSISFVAAEKQYMNAGFEGAVRSLSSYLNKYCPGGKFCSLALYYLADSYY
                     RTKDKENALTTYNKLLNMPGNPYMEEASLRSAEIAFDKKDYSSALVYFKKLQTVAQTT
                     ENSNIARLGILRCSYFLNNHAETEKIATEILSDPKTPEAVKSEAKLNRAKARAQQNKL
                     TDALKDLKEITIDTRTSIGAEAKFTLADVLFKLNRLNEAETEVLDFAKKGTPYQYWLA
                     SSFIVLADVYIQKGDDFQAKQYLLSLQKNYTVDDDIQPKINSRLTAIENRTKTKVIK"
     gene            1063..2802
                     /locus_tag="PKH68_08590"
     CDS             1063..2802
                     /locus_tag="PKH68_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13034.1"
                     /translation="MKTSILSLFLIVFSTGLIWAQDSLKNRNVTVEREYQPIINDAGK
                     IITTPRVIEPVVDKTKAVYSDISTPLSISTNIQTLPPEELEHLPTPAKKGFARVGLGY
                     PLNTLADFIYPLVKNENNRLDFTLRHLGALTDKIHSKTSASLQYDHLFPTFNFYTGVG
                     GRHDFYNYYGRVYGAEKSYGMADITPTYGNAIYTTPEDNTLTLRQLSGFPLDETNWRL
                     NFTAGARSLPDAESLHYDISANYNLFTAVTGKLTENQLLLKGNFDVPFNYNRLGMTVE
                     IHNLSYGSGVNKPVNFPLSYNLIKINPFYKLVGDEWFVRLGVKTGLSLGHGQVFTPSP
                     DVSAQWNAVPEYFSLYGGVTGDLAVNTLSRTYEINRYISPGNRLDDLYTPIDAFLGVK
                     LSPVYNLMFDVYGDYQIINNQYLFINRAYTTTETAVMPDIRNLYHNRFDVIYAPATKL
                     SAGMRVNYDYKNRYGLYAKGAYHSWNVENQEHAWQLPTWDADLGGYAKINNNISINTQ
                     FMFQDGRFARLGNTAHKMKPIYDWNLGASYAYLDWLSVFVKLNNILNSKYDQYSGYQV
                     QGINAMVGASFSF"
     gene            complement(2931..>3355)
                     /locus_tag="PKH68_08595"
     CDS             complement(2931..>3355)
                     /locus_tag="PKH68_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNX13035.1"
                     /translation="GATVNASNKIVLGNASATTVGGYGNWSNYSDRRLKENISYSNNL
                     GLDFILKLKTATYNYKADTNKRHRDGLIAQDVEQALKELGVNFSGLITDDDKDKTLNL
                     SYSDFVLPLINAVQELYKRNQVLESTVNELKAKMEISK"
BASE COUNT          999 a          799 c          688 g          869 t
ORIGIN      
        1 tcaaaaaagt ggtttcggat tatcccggca gcgaagaagc cgatgtggca ctcgaaagcc
       61 tcgagactat ctatgtggaa caaaatgaag tggatgatta tctgaactat acgcgatcac
      121 tgggacgaaa taccggtgat aataactctg cccgcgaaga ttccatctcc tttgttgcag
      181 ccgaaaaaca atatatgaat gccggctttg agggcgctgt tcggagtctg agcagctacc
      241 tgaacaaata ttgtcccggt ggaaaattct gcagcctggc tttgtattac ctggccgaca
      301 gctattaccg cacaaaagat aaggaaaatg ctctcacaac ttacaataaa ctgctgaaca
      361 tgcccggaaa tccatatatg gaagaagcgt ccctgcgttc ggccgagata gctttcgaca
      421 aaaaagatta ttcctcggcg ctggtttatt tcaaaaagct gcaaacagta gctcagacta
      481 cagagaacag caatattgcc cggcttggaa tactgcgttg ctcctatttc ctcaataatc
      541 atgcggagac ggaaaaaatt gccaccgaaa tactcagcga tccgaaaaca ccggaagccg
      601 taaaatcgga agccaaactc aaccgcgcaa aagcccgggc tcaacaaaac aaactgaccg
      661 acgctctgaa agatctgaaa gaaataacca tcgacacccg aacttccatc ggtgccgaag
      721 caaaattcac actggctgac gtgctcttca aactcaaccg gctgaatgaa gctgaaactg
      781 aagtactcga ttttgctaaa aaaggtacac cgtatcagta ctggctggca agcagtttta
      841 tcgtactggc cgatgtttat attcagaaag gcgatgattt tcaggccaaa caatacctgc
      901 tcagcctgca aaaaaattac actgttgacg acgatattca accaaaaatc aatagtcggt
      961 tgaccgccat tgaaaaccgg acaaaaacca aagtcatcaa ataatccctg aaaaaaagat
     1021 tattaatata aattagatat tcataaactc aactatattc caatgaagac cagtatatta
     1081 tcattatttc ttattgtatt ttccaccgga ctgatctggg cacaggacag cctgaagaac
     1141 cgaaatgtta ccgtagaacg cgaataccag cccatcatca acgatgccgg aaaaatcatt
     1201 accactccac gcgtcataga gccggtggtt gataaaacca aagcggttta ttcggatatc
     1261 agtacaccgc tcagcatcag taccaatata cagacattgc ctcccgaaga actggaacat
     1321 ctgccaacac ctgcaaaaaa aggatttgca cgtgtaggac tgggatatcc gctcaataca
     1381 ctggccgatt ttatttatcc actggtgaaa aacgaaaaca accggctcga cttcacgctg
     1441 cggcatctgg gtgcactcac cgacaaaatt cacagcaaaa cctcagccag cctgcagtac
     1501 gatcatcttt tcccaacatt taatttctat accggagtag gcggtcgtca cgatttctac
     1561 aactattacg gacgcgtgta tggtgccgaa aaatcgtatg gaatggccga tattacacca
     1621 acatacggaa atgctattta tactactcct gaggataata ccctcactct gcgccaactt
     1681 tcgggttttc cactcgatga aaccaactgg cggctgaatt ttaccgccgg tgcccgctca
     1741 ctgcccgatg ccgaaagtct tcactatgat atcagcgcca attataacct gtttacagcc
     1801 gtgaccggga aactgaccga aaaccaactg ttgttgaaag ggaattttga tgtgccgttc
     1861 aactacaacc gcctgggtat gacggtagaa attcacaacc tgagttacgg aagcggggta
     1921 aacaaaccgg tgaacttccc gttgagctat aatctgatca aaatcaaccc attctacaaa
     1981 ctggtgggcg atgaatggtt tgtccgactt ggagtgaaaa ccggactttc gcttggtcac
     2041 ggacaggtat ttaccccctc acccgatgtt agcgcccagt ggaatgctgt tccggagtac
     2101 ttttcactgt atggcggtgt taccggtgat cttgctgtca ataccctgag ccgcacctat
     2161 gaaataaacc gttacatcag ccccggaaac agactggatg atttgtacac tcccattgat
     2221 gcatttttgg gagtgaaatt aagccccgtg tacaacctga tgtttgatgt atatggcgat
     2281 tatcaaatta ttaataatca gtatctgttt atcaacagag catacaccac taccgaaacc
     2341 gccgtaatgc ctgatattag aaacctgtat cacaaccggt ttgatgtgat ttatgctccc
     2401 gccaccaaac tctcggccgg tatgcgtgta aattatgatt acaaaaaccg atacggactg
     2461 tatgccaaag gagcttatca cagctggaat gttgaaaatc aggagcatgc ctggcaactt
     2521 ccgacctggg atgccgatct gggaggatac gccaaaatca acaacaatat cagtatcaac
     2581 acccagttta tgtttcagga cggacgattt gcccggctcg gaaacactgc tcacaagatg
     2641 aaaccgattt acgactggaa tttgggcgca tcctacgctt acctcgactg gctttcggtt
     2701 tttgtcaaac tgaataatat tctgaattcc aagtacgatc agtacagcgg ataccaggta
     2761 cagggcatca atgcaatggt tggagcttct ttttcgttct gagagttgat atgaatataa
     2821 attaaaggct gtcgcggata aatttccaga cagccttttt ctattaaatg accggataca
     2881 tcaaatttca gaattcattt tttatgcatt ttgattggtt ctgataaaat ttacttcgaa
     2941 atttccattt ttgctttcaa ttcattcaca gtcgattcaa gcacctgatt tcttttataa
     3001 agttcctgta cggcattaat caacggcagt acaaaatcgg aatacgagag gttgagcgtt
     3061 ttatctttat cgtcatcggt aatcagtccg ctgaaattca ctccaagttc tttcagcgcc
     3121 tgctccacat cctgagcaat cagtccgtca cggtgccgtt tgttggtatc ggctttgtag
     3181 ttataggttg ccgttttcag tttcaaaata aaatcaagtc ccagattatt ggagtagctt
     3241 atattttcct tcagtctgcg gtccgaataa ttcgaccagt ttccataccc gcctaccgta
     3301 gtggcactgg cattacccag cactattttg ttgctggcat tcacagtggc accat
//
LOCUS       DAOPZK010000181         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_38354_length_3352_cov_24.381862, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000181 DAOPZK010000000
VERSION     DAOPZK010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38354_length_3352_cov_24.381862"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="PKH68_08600"
     CDS             <1..1278
                     /locus_tag="PKH68_08600"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNX13036.1"
                     /translation="FSSLADFILGLQLHKTSDDEPRKRKMWLYSSLAINFGILGFFKY
                     YNFFAESFVEAFTLFGRPLNYEPLKIILPVGVSFYTFQSLTYIIGIYKRKVEPTYRII
                     DFLAYVSFFPQLVAGPIERAAHFLPQFHSEKVFSYKNCQTGLLLIAFGLFKKIVIADR
                     LAVFVNGVYGDIPSAGGVSLLVAVLFFAFQLYFDFSAYSDIAIGSARLLGFELVTNFR
                     RPYLSKSFSEFWTRWHISLSSWFRDYVYFPLGGSRKGTRRTIINVMIVFLLSGLWHGA
                     SWNFVIWGGFNGLFLIIFDRIFKLEQPGNIKKYLAPVFIFCSWAVSLIFFRAQTFADA
                     LSVFTNAGLSGSDVLYDFGLKASEFRFTFFLLAAFILYELLAEKKGEKLAGWFYNKPG
                     FIRWSVYLLIVFTIIYLGAYGSSNDNTFIYFQF"
     gene            1283..2782
                     /locus_tag="PKH68_08605"
     CDS             1283..2782
                     /locus_tag="PKH68_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13037.1"
                     /translation="MTQPNKSKTRKNPSATPVKQPLKTAQTPVKPQTIYTLLVKAAIF
                     LVVVVAVVKFTDFKGYFNPDYTNDHTRRKWNAFYQFTKDKPVDVVLVGNSHLYTGINP
                     ENLSTALGANCFILASPGTTMTDVYYCLKEAIERNKPKVAVVETFGINDYTSHELKEG
                     TLTDQFKSFSARKNLRQKLCSTPVLFNSRNYLSAWSNTVRNHNFIFEDPIQIQTNIQL
                     NKQKQPEQTGLYLGRYIRFTSGLEDSTLMKYNTVVGFDGDKFAISREAEEFVAKTVQL
                     CRENDVKLVFLTLPMYHRHFKDMDAYISKVAKVLKPHQPLWENQQMPYDYNAFTSDCF
                     ENTVKENQHMTYYGSLVSTYKLARFIQTNLPGVLPDRSNDIEWKKLFYGYDGYFYNHR
                     PENDGVSQILAGNQTMGGLSVKGIELVPSQQGYKQLLVKLNKTSGASMTGKTIRVVLD
                     LNVNGKNQPAAIDVTSSYSCDPSSHYLFASQPLDYEMKAIALKEISLLN"
     gene            complement(2910..>3352)
                     /locus_tag="PKH68_08610"
     CDS             complement(2910..>3352)
                     /locus_tag="PKH68_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNX13038.1"
                     /translation="SRMYQLTGTDGFAEKYPVEGYCLRPSQVSTDVAPKHEDLNAHGF
                     VPEDVKTALMTKYKHPIHQELEETAKKVGGHGGMDYIMDYRLVYCLRNGLPLDMDVYD
                     LAEWCCMTELTAVSLENNSAPVEIPDFTRGAWNKVQGYRHAFVK"
BASE COUNT          890 a          750 c          709 g         1003 t
ORIGIN      
        1 ttcagctcgc tggccgattt tattctgggt cttcagctgc acaaaacatc ggacgatgaa
       61 ccccggaagc ggaaaatgtg gctttatagc agtcttgcaa tcaattttgg tattcttggt
      121 tttttcaagt attacaactt ctttgccgaa agttttgtgg aagcgtttac cctctttggc
      181 cgtccattga attatgaacc gctaaaaatc attctgccgg taggggtgag tttttatacc
      241 ttccagtcgc tgacttatat cattggcatt tataagcgga aagtggaacc cacttaccga
      301 attattgatt tcctcgcgta tgtaagtttt ttccctcagc tggtagccgg accaatcgaa
      361 cgggcagctc atttcctgcc acagtttcat tctgagaaag tattcagtta taaaaattgc
      421 caaacaggat tgctcctcat tgcttttggc ttattcaaaa agattgttat agccgaccgc
      481 cttgccgtat ttgtgaacgg agtatatggc gacatacctt ccgccggcgg agtttcactt
      541 ttagttgctg ttctgttttt tgctttccag ttgtattttg atttttctgc ctattccgat
      601 attgccatcg gttcagcccg gctgcttggt ttcgagttgg tgaccaactt ccgccgcccg
      661 tatctttcca aatctttttc agaattctgg acacgctggc atatttctct ttcaagctgg
      721 ttcagggatt atgtctattt ccccctgggc ggaagccgaa aaggaacacg ccgcaccatt
      781 attaatgtga tgattgtatt cctgctgagc ggtttatggc acggtgcatc ctggaatttc
      841 gttatctggg gaggtttcaa cggtttattc ctgataattt tcgaccggat tttcaaactc
      901 gaacaacccg ggaatattaa gaaatacctg gcacccgttt ttattttctg cagttgggca
      961 gtatcgctca ttttcttcag ggcacagaca tttgccgatg ccttgtcagt atttactaat
     1021 gcaggattat cgggcagcga tgtgctctat gattttggtc tgaaagcttc ggaattcaga
     1081 tttacatttt tcctgctggc agcatttatt ctgtatgaat tactggccga aaagaaaggt
     1141 gaaaaactcg ccggttggtt ctacaataaa ccgggcttca ttcgttggag cgtatatttg
     1201 ctgattgttt tcacaatcat ttatttgggt gcgtacggtt catccaacga taacaccttt
     1261 atttattttc agttctaaag ccatgacaca acctaataaa agtaaaacac ggaaaaatcc
     1321 ttcagctacg ccggttaaac agcctttaaa aactgcgcaa acaccggtaa aaccacaaac
     1381 catatatacc ctgttggtta aggcagctat tttccttgtt gttgtggtgg cggtagtgaa
     1441 gtttaccgac ttcaaaggct attttaatcc cgattacacc aacgaccata cccggcggaa
     1501 atggaatgcc ttttatcagt ttacaaaaga taaacccgtg gacgtcgttt tggtaggcaa
     1561 ctcgcatctc tacaccggca tcaatcccga aaacttatcc actgcgctcg gagccaattg
     1621 ttttattctg gcctctcccg gcactaccat gaccgacgtg tattattgcc tgaaagaagc
     1681 cattgagagg aacaaaccaa aagtggcggt ggtggaaact ttcggaataa acgattatac
     1741 atcgcacgag ttgaaggagg gtactttgac cgatcagttt aagagtttct ctgcccgaaa
     1801 gaatttacgg caaaaactct gctccacgcc ggtgcttttc aattccagaa attacctgtc
     1861 ggcctggtca aatacggttc gcaatcataa ttttatcttc gaagatccca tacagataca
     1921 gactaatatt cagttgaata aacaaaaaca acccgaacag accggacttt atctgggtcg
     1981 atacatccgt tttacttccg ggcttgaaga cagcacgctg atgaagtaca acacggtagt
     2041 aggatttgat ggtgataagt tcgctataag ccgggaagcc gaagaatttg tagccaaaac
     2101 ggtacaactt tgcagggaaa atgacgtgaa gctggttttt ctaacattac ccatgtatca
     2161 ccggcatttc aaagacatgg atgcctatat ttccaaagtg gctaaggtgt tgaaaccaca
     2221 tcagccgctg tgggaaaatc agcagatgcc ttatgattac aacgctttta catccgattg
     2281 tttcgaaaat acggtgaagg aaaaccagca catgacctac tacggttcgc tggtgtcaac
     2341 ttataaactg gcccggttta tccaaactaa tttacccgga gtactgcccg acagaagcaa
     2401 tgacatcgaa tggaaaaaac tgttttacgg atacgacggc tatttctaca atcaccgacc
     2461 cgaaaatgac ggggtcagtc agattttggc aggtaatcag acgatgggcg gactgtcggt
     2521 taagggaatt gaacttgttc cttcgcagca aggctacaaa caattgcttg tcaagctaaa
     2581 taaaacttca ggtgcatcca tgacaggtaa aacaattcgt gtggttctcg atttgaatgt
     2641 taacggaaag aatcaacctg ctgcaattga cgttacctcc tcatactcat gtgacccttc
     2701 atcacattac ctgtttgctt ctcagccgct tgactacgaa atgaaagcca ttgctttgaa
     2761 agaaatcagt ctgttgaact aaactgtttt caaattgcaa aaccgatgat taatgatttt
     2821 tttgttattg atttggctga attaaaacaa taaaaaatgt cccgaaacat attattccgg
     2881 gacatttttt tatttattct aatgttgatt tatttcacaa aagcatgtct gtagccctgc
     2941 accttgttcc aggctccgcg tgtgaagtcc gggatttcta ccggtgcact gttattttcc
     3001 agcgaaacag cggtcagttc tgtcatgcag caccattcag ccaggtcata cacatccata
     3061 tccagcggaa gtccgttgcg caggcaatac accaggcgat aatccatgat atagtccata
     3121 ccaccgtgac ctcccacttt cttggctgtt tcctccagct cctgatggat agggtgtttg
     3181 tatttcgtca tcagtgctgt ttttacgtct tctggtacaa aaccatgagc gttaaggtct
     3241 tcatgttttg gagcaacgtc ggttgaaacc tgtgacgggc gcagacagta tccttctacc
     3301 ggatattttt cagcaaaacc atcggtgccg gtcagctgat acatacggct gt
//
LOCUS       DAOPZK010000182         3339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_38580_length_3339_cov_31.879415, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000182 DAOPZK010000000
VERSION     DAOPZK010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38580_length_3339_cov_31.879415"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="gnd"
                     /locus_tag="PKH68_08615"
     CDS             <1..1284
                     /gene="gnd"
                     /locus_tag="PKH68_08615"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNX13039.1"
                     /translation="SIEELCNSLERPRKVMMLVKAGKAVDDFIDLLIPHLEEGDIIID
                     GGNSHFPDTIRRTKYVESKGLLYIGTGVSGGEEGALLGPSMMPGGSPAAWPAVKDIFQ
                     AVAAKVEGDVPCCDWVGEDGAGHFVKMVHNGIEYGDMQIINEAYQVMKDLLGMTADEM
                     HEVFKEWNEGDLDSYLIEITRDILAFKDEDGEPLVEKILDTAGQKGTGKWTAVSALDL
                     GIPLTLIGESVFARCLSAQKDMRVLASKSISGPAPTFNGDRKQMIADLKDALFGAKII
                     SYAQGYNLMMEAANEYKWNLNYGGIALMWRGGCIIRSVFLGDIKKAFDNNPKLDNLLL
                     DPYFKNIVEKAQAGWRRVCAAALTNGIPVPALTSALCYFDGLRTERLPANLLQAQRDY
                     FGAHTYERIDKPRGEFFHTNWTGRGGDTASTTYVV"
     gene            1738..2091
                     /locus_tag="PKH68_08620"
     CDS             1738..2091
                     /locus_tag="PKH68_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNX13040.1"
                     /translation="MTNLSLFVVVLLSGMTLVLGGLGLALLISPKSYNFQKGEPYECG
                     LPTHGTSWMQFRVGYYLYAILFLMFDVETIFLFPWSTVVRSLGVMGLVSILIFIIILG
                     LGLAYAWRKGVLKWT"
     gene            2082..2723
                     /locus_tag="PKH68_08625"
     CDS             2082..2723
                     /locus_tag="PKH68_08625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNX13041.1"
                     /translation="MDVNKIYIKGIAGEDFKDNDTLQQYIDELNAGGTNVIVGKLDEL
                     IDWGRANSLWPLAFATSCCGIEFMAAAAAHYDLARFGMEVTRNSPRQADLMVVAGTIV
                     NKMGPLLRRVYDQMAEPKYVVAMGGCAISGGPFVNSYNVVNGADKIIPVDVYIPGCPP
                     RPESLFYGLLQLQRKVKVDRFFHNQRPVREYNPDTLIDPKTGEIIDTKTSPEK"
     gene            2753..>3339
                     /locus_tag="PKH68_08630"
     CDS             2753..>3339
                     /locus_tag="PKH68_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNX13042.1"
                     /translation="MKDIKELILHAVSDAVIEEKQKLTVTVEPKQLHPLMQALYNNKD
                     LPFDYLISLIGMDWGEKLGVIYLLSSSKDLTKELVVITATADRENPLLYSITDLYETA
                     HLNEREAYALFGIRFINNPDMRRFLLNDDWVGFPFRKDYNDDAKLNPVNIITKDIPDM
                     SPRIMETADGKLIEETVPVFEEDDYIINIGPQHPST"
BASE COUNT          971 a          692 c          736 g          940 t
ORIGIN      
        1 tcaatagaag aactctgtaa ttcactcgaa cgtccgcgca aagtgatgat gctcgtaaaa
       61 gccggaaaag cggtggatga ttttatcgac ctgttaattc cgcacctgga agaaggcgat
      121 attattattg acggtggaaa ttcacatttc cctgacacca tacgtcgaac gaagtatgtt
      181 gaaagcaaag gattacttta tatcggaacc ggtgtttcgg gcggtgaaga aggcgccttg
      241 ctcggtcctt caatgatgcc cggcggatca cccgcggcat ggcctgctgt gaaggatatt
      301 ttccaggcag tagctgcaaa agtggaaggc gatgtaccct gctgcgactg ggtaggcgaa
      361 gatggtgccg gacattttgt aaaaatggta cacaacggca ttgaatacgg cgatatgcag
      421 ataatcaatg aagcgtatca ggtaatgaaa gacctgttgg gcatgactgc agacgaaatg
      481 cacgaggttt tcaaagaatg gaatgaaggt gatttggact catacctgat agaaattacc
      541 cgcgatattc ttgcatttaa ggatgaagac ggagagccgt tggtagaaaa aattctggat
      601 acagcaggtc aaaaaggaac cggtaaatgg acagctgttt cggcgcttga ccttggtatt
      661 ccattaacgt taatcggcga gtcggtattt gcacgttgct tgtctgctca gaaagatatg
      721 cgtgtactgg catcaaaatc catcagcgga ccagcaccta cattcaacgg agatcgtaaa
      781 caaatgattg cagacctgaa agatgcactg ttcggagcaa aaatcatttc gtatgctcag
      841 ggttacaacc tgatgatgga agctgccaac gaatataaat ggaacctcaa ttacggcggc
      901 attgccctga tgtggcgtgg tggctgcatc atccgttccg tctttttggg cgatatcaaa
      961 aaagcattcg ataataatcc taaactggat aatctgttgc ttgaccccta tttcaaaaac
     1021 atagtggaaa aagcccaggc cggatggcgc cgcgtttgtg cagctgcact caccaacgga
     1081 attcctgtgc ctgctctaac ctctgcactt tgctacttcg acggactgcg taccgaacgt
     1141 ctgcctgcca acctgttgca ggcacaacgg gattatttcg gagctcatac ctatgaacga
     1201 attgacaagc ccagaggaga atttttccat accaactgga caggccgtgg tggcgatacc
     1261 gcttcaacaa cttatgtggt ttaaaaaatt agattagatt ggtggttttt ttagattagg
     1321 ttggaaatcc ggattattca gttaatccgg attttatttt tttgagcagt taatttctcg
     1381 tgtatttcat ttttctgtat taaaatcatt ttttcgacat ttattcgtaa aaccagaagg
     1441 attactttca tatattaaat tatttaatta attattcttc attttgaaat ttcatcttta
     1501 taacataaaa taatttgaac ctcaaaatac cttaatttgc aaactccact ttacaaaatt
     1561 aactatttta tattcttcat tccaaaaaat attccttaca tttgcacaaa atttctgaca
     1621 ggctttatat aatccacctg atatactgaa aaatcgagct aatactatgg taaacacccg
     1681 tttacaactc tagcaatttc agtaatccgg tattcaaaaa acaaattata aaacagcatg
     1741 actaatttat cactattcgt ggtagtgctt ctctcaggaa tgacgctggt tctcggcggt
     1801 cttggattgg ccttactaat ttccccaaaa tcttacaatt ttcaaaaagg agagccttat
     1861 gaatgcgggc ttccaacaca cggtacatcc tggatgcagt tcagagttgg ttattacctc
     1921 tacgctatcc tgtttttgat gttcgatgtt gaaactattt tcctcttccc ctggtccacc
     1981 gtggtacggt cgcttggtgt aatgggactg gttagtattc tgatatttat cataattctc
     2041 ggcttaggtc ttgcctatgc atggaggaaa ggagtgttga aatggacgta aacaagattt
     2101 acataaaagg aattgcaggc gaagatttta aggacaatga cacgctgcaa cagtatattg
     2161 atgagttgaa tgccggtggt acgaatgtta tcgttggtaa actggatgaa ctgattgact
     2221 ggggtcgtgc caattctctc tggccgctgg cttttgccac cagttgctgc ggtattgaat
     2281 ttatggcagc tgctgctgct cattatgatt tggcccgctt tgggatggaa gtaacacgta
     2341 acagccctcg tcaggccgat ttgatggttg ttgccggaac cattgtgaat aaaatggggc
     2401 cgctgcttcg ccgtgtatat gaccagatgg cagaaccaaa atatgtggtt gcaatgggtg
     2461 gatgtgccat ttcaggaggt ccgttcgtta attcatacaa tgtggtaaac ggtgctgata
     2521 aaatcattcc ggtggatgtt tacattcccg gatgtccgcc ccgtcccgag tcgctgttct
     2581 atggattgct gcaattgcaa cgcaaggtaa aagttgaccg gttcttccac aaccagcgtc
     2641 cggtacgtga atacaatccc gacacactga ttgacccgaa aacaggggaa attatcgaca
     2701 caaaaacaag tcctgaaaaa taaaactata aaaatcatca tattccaata agatgaaaga
     2761 tataaaagag ctaatactac atgcagtaag cgatgcggtc atcgaagaaa agcagaaact
     2821 gaccgtgact gttgagccga aacagcttca tcccttaatg caggcattat ataataataa
     2881 agatcttcct ttcgactacc tgataagcct tattggtatg gattggggcg aaaaactggg
     2941 tgtgatctat ctgttgtcat cttccaaaga cctgacaaag gaactggtag tcataacagc
     3001 aaccgccgat cgtgaaaatc cgttgttgta ttccattacc gatctttatg aaaccgctca
     3061 tctcaatgaa cgcgaagcat atgcactatt cggcattcgt tttatcaaca atccggacat
     3121 gcgccgcttt ctgctcaacg acgactgggt gggatttcca ttccgtaaag attacaacga
     3181 tgacgcgaaa ctaaatcccg ttaacatcat cacgaaggat atccctgata tgtctccacg
     3241 cataatggaa acagcagatg gcaagctcat tgaagaaacg gttccggttt tcgaagaaga
     3301 tgattacatt ataaatatag gtcctcagca cccttccac
//
LOCUS       DAOPZK010000183         3313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_39041_length_3313_cov_15.622468, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000183 DAOPZK010000000
VERSION     DAOPZK010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39041_length_3313_cov_15.622468"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="PKH68_08635"
     CDS             complement(<1..1786)
                     /locus_tag="PKH68_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNX13043.1"
                     /translation="MKVYQTNEVKNISLLGSSGAGKTTLVEAMLYESGVIKRRGSING
                     QNTVSDYFPVEKEYGYSVFSSVLSYEWKNKMMNFIDCPGSDDFVGSVISALNMTDMSL
                     MLIDAANGVEVGTMNQFRYTEELHKPLVFLINQLDHEKADYDNTLANLKETYGSKVVP
                     IQYTIGSGQSFDAIVDVLKQKMYKWKPGATAPEVLDIPESEKDKAGEFYQVLLEAAAE
                     NDENLMEKYFEQGSLTEEEMREGIMKGMIDRSLFPVFCASGEKNMAVHRIMNFLSMVA
                     PSPDQMPAPVNRAGKEVKPDAKGPTSLFFFKTTVEPHIGEVSYFKVMSGKVKPGDDLM
                     NVSRSSKERITQIYSVSGQIRTAVDEMVAGDIGATVKLKDTRTGNTLNEKGCDYDYKA
                     INYPEPKFRRAIKAVSESDEEKLNEILTRMHEEDPTWVIEQSKELKQTILSGQGEFHL
                     RTLKWRVENNDKILIEYKEPKIPYRETITKVARADYRHKKQSGGAGQFGEVHLIVEPY
                     YEGMPAPDMFRFNGQEYKISVRDTQVVDLEWGGKLVVINSIVGGAIDARFIPAIMKGL
                     LDRIEQGPLTGSYARDVRVIVYDGKMHPV"
     gene            complement(2063..2797)
                     /locus_tag="PKH68_08640"
     CDS             complement(2063..2797)
                     /locus_tag="PKH68_08640"
                     /inference="COORDINATES: protein motif:HMM:NF040365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6261 family protein"
                     /protein_id="HNX13044.1"
                     /translation="MEKIRKVKMTSLHHNEHAEFHKAIVREIKTNTPASLGIEKLFPA
                     YEAALENELTAIDVENGSRHTVAIEEADIYRDEMHQSLVLETRSKLVSYKQENREAGA
                     QVKRILDQVGDLRKKPYNQETETIQSLVNQLKENYADELESTGLIEAVNQLDIANKSF
                     NDRFGERATDVSGRPNGDVIVERVVVDETYRNITDAINGLAIINGDTAYRAFMDKVNY
                     LVDYYKTTLSQRRANKKATVTTPSIQ"
BASE COUNT          828 a          729 c          659 g         1097 t
ORIGIN      
        1 ctacggggtg catcttacca tcgtaaacaa tgacgcgaac atcgcgggca taagaaccgg
       61 taagaggacc ttgttcaatg cggtcgagca atcctttcat aattgcaggg atgaaacgtg
      121 cgtcaatagc accaccgaca atactattga ttaccaccag ttttccaccc cattcgaggt
      181 caacaacctg cgtatcgcgt acacttattt tgtattcctg accattgaag cggaacatat
      241 cgggtgcagg cattccttcg tagtaaggtt caacaattag gtgaacttca ccaaactgtc
      301 cggcaccgcc cgattgtttt ttgtgacggt aatcggcacg tgcaaccttc gtgatggttt
      361 ccctgtaagg aattttcggt tctttatatt caatcaggat tttgtcgttg ttttcaactc
      421 tccatttcag tgtgcggaga tgaaattccc cctgtcccga caagatggtt tgtttcagtt
      481 ctttcgattg ttcaataacc caggttggat cttcttcatg catccgtgtc aggatttcat
      541 tcagcttttc ttcgtcggac tcagaaacag ctttgatggc acgacggaat tttggttcgg
      601 ggtaatttat tgccttataa tcataatcac accctttttc attcagggta ttgcccgtac
      661 gggtatcttt aagttttacg gttgcaccga tatcaccagc caccatttca tccactgcgg
      721 ttctgatttg tcccgataca gaataaatct gtgtaatacg ttctttagaa ctacggctca
      781 cgttcatcag atcgtcacct ggtttcactt tgccactcat caccttgaag tatgaaactt
      841 cgccaatgtg tggttcaacg gtagttttaa agaaaaacag gctggttggt ccttttgcat
      901 cgggtttcac ttcttttccg gctctattaa ccggcgcggg catttgatcg ggtgatggag
      961 caaccatgct caggaaattc ataatacgat gaactgccat atttttttca cctgaagcgc
     1021 agaaaaccgg gaacaacgat cggtcaatca tacctttcat aataccctca cgcatttctt
     1081 cttcagtgag tgaaccctgt tcgaagtatt tttccatcag attttcgtcg ttttcagcgg
     1141 ctgcttccaa caaaacctga taaaattcac cggctttatc tttttcgctt tcaggaatat
     1201 caagaacttc cggcgctgtt gctccgggtt tccatttgta cattttttgt ttaaggacat
     1261 ccactatggc atcaaaagat tgtccgctgc ctatagtata ttgtatcgga acgactttgc
     1321 ttccgtaggt ttctttcagg ttggcaagtg tattatcata atcagctttt tcgtgatcca
     1381 gctggttaat aagaaataca agcggtttat gcaattcttc ggtataacgg aattggttca
     1441 ttgtacctac ttccactcca ttagccgcgt caatcagcat caatgacata tccgtcatgt
     1501 taagagcaga aatgactgag cccacaaaat catcagaacc gggacagtca atgaaattca
     1561 tcattttatt cttccattca tacgacaaca cactcgaaaa aacagaatat ccgtattctt
     1621 tttctacagg aaaataatcc gaaactgtgt tttgaccatt aattgatcct ctgcgtttta
     1681 taactccact ctcataaagc atggcttcca cgagagtggt tttcccagcg ccagaactac
     1741 ccaacaagga gatgttctta acttcattag tttgatatac tttcatataa cgttaattta
     1801 gaaatgatta tgtaagaatt attttataat acacgtaatt aatatcgaca tgcaatttat
     1861 aaaaacaaaa ttataaatta ctgttatttt acatgtttta caacataatt ttaagcctga
     1921 ttgcgggaat aacggaagtc tgacatcggg caaatgattt taatcatttt tttcgacaaa
     1981 aaagtttatc catttattaa aacagacact gccggagcgc ttaactccgg cagtgcgtgg
     2041 cattatttct tttcttctcc gattactgga ttgagggcgt tgttacggtt gctttcttat
     2101 ttgcccttct ttgcgataag gtggttttat aataatctac caggtagttt accttgtcca
     2161 tgaaggcgcg gtaagcggtg tcgccgttga tgattgccag cccgttgatg gcatcggtaa
     2221 tattccggta agtttcgtcc acgacaaccc gttctactat cacatcgccg tttgggcgtc
     2281 ctgaaacatc cgtggcgcgt tcaccaaaac ggtcattgaa acttttgttg gcaatgtcca
     2341 gttgattgac agcttctatc agtcccgtgc tttccagttc gtcagcataa ttttccttca
     2401 gctgattcac cagactctga atggtttccg tttcctgatt ataaggtttt tttcgcaaat
     2461 cgcccacctg gtcgagaatg cgtttgacct gtgcacccgc ttcgcggttt tcctgtttgt
     2521 agctcaccag ttttgatctg gtttccaaca ccagtgactg atgcatttca tcgcggtaga
     2581 tatccgcctc ttcgatggct acggtatgcc tgctgccatt ctccacatca atggcggtca
     2641 gctcattttc cagggctgct tcgtaagccg gaaaaagttt ttcgatgccc agcgatgcag
     2701 gcgtattggt tttaatttcc ctcacaatgg ctttgtgaaa ttctgcgtgc tcgttgtggt
     2761 gcaggcttgt catttttact tttcgaattt tttccaaaat tgtattgttt aaaggttata
     2821 catgtatatt catgcaacaa atataaatat tgttttttta aaatgcaaat ttttagccga
     2881 aattttgcgg gaagggattt ccccgggaaa tatcttgtcg cagttttgcg ggaacaaatt
     2941 tccccggtat atttgggcgc tgcagttttg caggaagaaa tttccccggg aaatatgttg
     3001 tcgcagtttt gcgggaagaa atttccccag tacattttgc cgccgcagtt ttgcgggaag
     3061 agatttcccc gggaaatgtg ttgccgcaat tttgcggctg gaaaatgtac tttttatttc
     3121 tggtcactat caattcagag cctgtacgac cgttttctga ttagtggcac ggttattctt
     3181 tgggcaaaag atttttttcc ggacaaaaaa aagtttatca atgaatatcg ataaactttt
     3241 tcttccaaaa caggagtctt attatttctt ggtataaacc atttgtttcc gttcttcact
     3301 tactgctttg cgt
//
LOCUS       DAOPZK010000184         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_39167_length_3305_cov_25.268000, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000184 DAOPZK010000000
VERSION     DAOPZK010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39167_length_3305_cov_25.268000"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /gene="ileS"
                     /locus_tag="PKH68_08645"
     CDS             complement(<1..1817)
                     /gene="ileS"
                     /locus_tag="PKH68_08645"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNX13045.1"
                     /translation="MKNFKEYSQLNLSEVNKKMLEYWSKHDVFHKSIETREGHPTFVF
                     YEGPPSANGMPGIHHVMARTIKDVFCRYKTMQGFQVKRKAGWDTHGLPVELGVEKSLG
                     ITKEDIGKTISVEEYNAACRHDVMKYTREWEDLTEKMGYWVDMNDPYITYDNRYIETL
                     WWLLKELYKKGLMYKGYTIQPYSPAAGTGLSSHELNQPGCYRDVKDTTCIAQFKLKNK
                     DNTYFLAWTTTPWTLPSNTALAVGPNIDYVFVKTHNPYSKKEVSLILAENLLLSVMGK
                     NEYEIIDHCKGSDLVGLEYEQLIPWVNPGEGAFRVIPGDFVTTEDGTGIVHIAPTFGA
                     DDDRVAKQTGVPPLMMLDREGNRQPMVDKSGKFYLTDDLDKDFVSEFVNTDLYAEFAG
                     RYVKNSYNEALGEADETLDIAICMMLKQQGLAFKIEKHIHSYPHCWRTDKPVLYYPLD
                     SWFIRSTACREEMIALNNTINWKPQSTGTGRFGKWLENLQDWNLSRSRYWGTPLPIWR
                     TDDGQEEICIGSAEELMGEIEKSIAAGYMTENPFRNFKPGVYTAENYDVKNIDLHRPY
                     VDGIVLVSASGKPMKRETDLIDVWFDSGAMPFAQLHYPF"
     gene            complement(2092..3033)
                     /locus_tag="PKH68_08650"
     CDS             complement(2092..3033)
                     /locus_tag="PKH68_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNX13046.1"
                     /translation="MTILLIIIAFFVLLVIFTGLVTVNQGYVAVTTIFGKYSRILGPG
                     LNFKIPFIELVHRRISIQNRSVELEFQAVTQDQANVYFKAMLLYSVLNQSEDVVKNVA
                     FKFVDDRNFMQALIRTIEGTIRSFVATKKQAEILGLRTEIILEVKKHLDDTLESWGYH
                     MIDIQLNDITFDEEIIKSMARVVASNNLKAAAENEGQALLITKTKSAEADGNAIKISA
                     LAEKEAAMQRGQGIALFRQEVARGMAQAAQEMTVANLDASYLLFSMWTEAIKHFAETG
                     KGNVMFLDGSTNGMENTMKQMMAMMKLGEPTVAEKKK"
     gene            3213..3300
                     /locus_tag="PKH68_08655"
     tRNA            3213..3300
                     /locus_tag="PKH68_08655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3247..3249,aa:Ser,seq:gga)
BASE COUNT          885 a          739 c          702 g          979 t
ORIGIN      
        1 ctaaacggat aatgtaattg tgcaaaaggc atagctccgc tatcaaacca aacatcaatc
       61 agatccgttt cccgtttcat gggctttccg gatgccgaaa ccaacacgat accatccaca
      121 tacgggcgat gtaaatcgat gttctttaca tcataatttt cggccgtata aactcccggt
      181 ttgaagtttc taaacggatt ttcggtcatg tatccggctg caatggattt ttctatttca
      241 cccatcaatt cttcggcaga gccaatgcaa atttcttcct gcccatcgtc ggtgcgccat
      301 attggcagcg gagttcccca gtagcggctg cgacttaggt tccagtcctg aaggttttcc
      361 aaccatttac cgaaacgtcc tgtaccggtg gactgcggtt tccagttgat ggtattgttt
      421 agtgctatca tttcttcgcg gcaggcagtc gagcggataa accagctatc gagcggatag
      481 taaagcaccg gcttgtcggt acgccagcaa tgaggataac tatggatatg tttttcaatt
      541 ttgaaagcca gtccctgttg tttcagcatc atacagatgg caatatcgag tgtttcatcg
      601 gcttcaccca acgcctcatt gtaagagttt ttcacatatc ttccggcgaa ttcagcatat
      661 aagtcagtat ttacgaattc cgacacaaaa tctttgtcca gatcatctgt taagtagaat
      721 ttgcccgatt tatccaccat aggttggcgg ttgccctccc tatcaagcat catgagtggt
      781 ggcacaccgg tttgctttgc cacgcgatca tcgtcagcac caaaagtggg agcaatatgt
      841 acaattccgg taccatcttc ggtagtgaca aagtcacccg gaatgacacg gaaagcacct
      901 tcacccggat ttacccaggg aatgagttgt tcatactcca gtcctacaag atcagaacct
      961 ttgcaatgat ctataatttc atattcattt ttacccatta ccgaaagcag tagattttcg
     1021 gccaatatca gactgacttc ctttttggag tagggattat gagttttgac gaacacatag
     1081 tcgatattgg gacctacagc cagagctgta ttcgaaggca atgtccaggg agtggtagtc
     1141 caggcaagaa aatatgtgtt gtctttgttt ttcagtttaa actgcgctat gcaggtggta
     1201 tccttcacat cgcggtaaca gcccggctgg ttcagctcgt gtgagctcag accggttccg
     1261 gcagcgggtg agtaaggctg gatggtgtat cctttgtaca tcaatccctt tttatacaac
     1321 tctttaagca gccaccagag agtttcgatg tagcggttgt cgtaggtgat atacggatcg
     1381 ttcatatcca cccagtaacc cattttttcg gttaagtctt cccattcgcg ggtatatttc
     1441 atcacatcat gacggcaggc agcattgtac tcttccacac tgatggtttt accgatatct
     1501 tcttttgtaa ttccgagtga tttttccaca cccagttcta cgggcagtcc gtgggtatcc
     1561 cagcccgctt tgcgcttcac ctgaaatccc tgcatggttt tatagcggca gaacacatcc
     1621 ttgatggtac gcgccatcac gtgatgaatg cccggcatgc cgttggccga aggcggacct
     1681 tcatagaaaa caaaggtggg atgaccttcg cgggtttcaa tgcttttgtg gaatacatca
     1741 tgtttactcc agtattcaag cattttttta ttaacttcgg ataaattcag ctgcgagtat
     1801 tctttaaagt ttttcatcgg aaaaagttcg aattattttc attgttacga attgaaaatc
     1861 cggctgtgat caactatcta tacaataatg tatggttatt gactaataag cctgattttg
     1921 agggtgcaaa attacaaaaa aatatggaaa tggaaaagta agaaactgat aactatcaga
     1981 ccgacagttc atgctaagtt tttgatattc attcgtcgga aggtgtaaaa aatagtacct
     2041 gtttttcggt gacgaacaac aggtacattc tgtaagtaat cgcttaataa cttatttttt
     2101 cttttcagcc acagtcggtt ctccgagttt catcatggcc atcatctgtt tcatggtgtt
     2161 ttccataccg ttggtagaac cgtcgagaaa catcacatta cccttgcctg tttcggcaaa
     2221 atgcttgatg gcttctgtcc acatggaaaa gagcagatac gaagcatcga gattggcaac
     2281 agtcatttcc tgcgctgcct gagccatccc tctggccact tcctgacgga agagtgcgat
     2341 accctggccg cgttgcatgg cggcttcttt ttcggccaga gccgagattt tgatggcatt
     2401 accatccgct tcggccgatt ttgttttggt tataagcaaa gcctgacctt cattttcagc
     2461 agccgctttg aggttgttgg atgccaccac gcgtgccatc gatttgatga tttcttcatc
     2521 gaaggtaatg tcattgagct gaatatcaat catgtgatat ccccagcttt cgagggtatc
     2581 atccaggtgt ttcttcactt ccagaataat ttcggtgcga agtcccagga tttcagcttg
     2641 ctttttggtg gcaacaaaac tacggatagt accttcgatg gtgcggataa gcgcctgcat
     2701 aaaattacgg tcatccacaa atttgaaagc gacgtttttg accacatctt ccgactgatt
     2761 gagcaccgaa taaagcaaca tggccttaaa atacacattg gcctgatcct gtgtaacagc
     2821 ctgaaactcg agctcgaccg atcggttctg gatggaaata cgtctgtgaa ccaattctat
     2881 aaacggaatc ttgaaattta gtccgggacc caaaatgcgg ctatacttac caaaaatagt
     2941 ggtaactgcc acataaccct gattgaccgt taccaaaccg gtgaaaatga caagaagcac
     3001 aaaaaacgca atgataatta atagaatagt catgttataa ttttatttag tgtgaataaa
     3061 aatgtgaaca cgcaaagata tgcaatttta cagtcaatca ttaattttaa caataaatct
     3121 tacagatgtt attaaaaaat tattcacttt caattccgga taaaattttc atattccggt
     3181 ttctttttat acatttgcag tcccaaaacg aaggagagat gctcgagtgg tttaagaggc
     3241 acgcttggaa agcgtgtgta ctccaaaagg gtaccggggg ttcgaatccc tttctctccg
     3301 caaat
//
LOCUS       DAOPZK010000185         3301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_39239_length_3301_cov_20.617991, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000185 DAOPZK010000000
VERSION     DAOPZK010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39239_length_3301_cov_20.617991"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1166)
                     /locus_tag="PKH68_08660"
     CDS             complement(165..1166)
                     /locus_tag="PKH68_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNX13047.1"
                     /translation="MKVFRDYFFFIGIAILFFMAYKLGWKTIVDNLGQTGWWFLAIVG
                     VWIFVYLANALSLAIIIRDGSEESKRVSLMHLLKLTISAYSINYITPLAIGGEPYKAL
                     ELKPDLGTHKATSSVLLYVMMHYVSHFFFWMISVPLFLLIIPKIPPGVQIILWGMIFG
                     SILLIYWAFTVYTKGIIRKALAIGCRIPFFGKKIRIYRDKNKERFDEMDELIANLFTN
                     RKKDFFLSLGVELLSRYISCLEIYFMVIPMNYDITFIQCILIYSFATLVANIFFFAPM
                     QMGTREGGFALAVSLLYVPAGLGVYIGLCTRIREFFWIVVGIGLMKLKIPDRLQIRS"
     gene            complement(1194..2072)
                     /locus_tag="PKH68_08665"
     CDS             complement(1194..2072)
                     /locus_tag="PKH68_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNX13048.1"
                     /translation="MKTEIVASLKSIETENYLDRIFYRPIGFKIAKALIGTGITPNMI
                     TILSLFVGSVAGVLWYFPYSLTFAVVGILSLIVANIMDCVDGQLARLTGIKSEIGRIL
                     DGFAGDFWFLLIYIAFAHRLNVQFDTWLFWLAALLMAISHLNQAAITDYYKTLHLFFI
                     SKEKGKEFESSERIRARIATMPAGVNRFFSRFYLIYTLSQEKQTPEVQKYLAALKEKY
                     GEDIPEDVRLAFRTKSRQLLPMIDMMTFNGLSGILFLSVLLNAVWIYLVWVIVILNIV
                     NKIIRLRHEKMCKSLG"
     gene            complement(2172..>3301)
                     /locus_tag="PKH68_08670"
     CDS             complement(2172..>3301)
                     /locus_tag="PKH68_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX13049.1"
                     /translation="YEGQKIDDILRYGVDIFAIGSDWLGKFDYLKEYCQVVYLERTKG
                     VSSTQLRIEAQSVIELGIIGSGRIANRFIKESKYVSGIEVKGVLNPKIESAKSFCEKN
                     ELNFYCTEYEDFIARVDAVYISSPHLTHYDYIRRSLEHGKHVLCEKPMVLSAGEAEEL
                     YKLAEDKGLVLKEAIKTAYCPAFAHLITVVKSGVIGNIKDIDVSYSKLTTGSVRELDA
                     AQAGGSITELASYVLLPIIKILGMEYEDIHFYSHVHNDVDLFTKGIVLYKHATASFNI
                     GLGVKTEGDLVISGTKGYAYVPAPWWNTEYFELRYEDLNQTRKYFYKFDGDGLRYEIN
                     DFLIEMNNPHWKQYKFMNSESIVIAAIIERYRSGLHVHKIL"
BASE COUNT          997 a          712 c          670 g          922 t
ORIGIN      
        1 cgctcctgat aggtgcgtac aatgcccgaa aatgcagtcg ctttgtcttt gtgggacaat
       61 gagttgaatg aaatattgct tgaattcaat tgtttgcttt tagaggttag acttgcaaag
      121 ccccaaaagg tttaatcggg atcagtataa aagaactgta aagtttagct tcggatttga
      181 agtctatcgg ggattttcag cttcatgagt ccaatcccca ccacaatcca gaaaaactcg
      241 cggatacggg tgcatagtcc aatgtaaact cccaacccgg caggcacgta caacaggctg
      301 actgccaatg caaaaccacc ttcacgcgtc cccatctgca tgggcgcaaa gaagaatata
      361 ttagcaacaa gagtagcgaa tgaatagata agaatacact ggataaacgt aatatcgtaa
      421 ttcatgggaa tgaccataaa atatatttca agacatgaaa tgtaacggga aagcaactcc
      481 acgcccagcg acaagaagaa atccttcttc cggtttgtaa aaagattggc gattagttca
      541 tccatttcat caaaccgttc cttgttctta tccctgtaga ttctgatttt tttcccgaaa
      601 aacggaatcc ggcaacctat tgccagtgct ttacgtataa tccctttggt ataaacggta
      661 aatgcccagt aaatgaggag gatgcttcca aagatcattc cccagagaat tatctgtact
      721 ccgggtggaa ttttgggaat aataagcaaa aagagtggaa cagaaatcat ccagaagaaa
      781 aaatgcgaga cataatgcat catcacataa agcagaacag aagaagtggc tttgtgggtt
      841 cccagatcgg gtttaagttc gagcgcttta taaggttcac cgcctatggc aagtggtgtg
      901 atataattga tactatatgc cgagatcgtc agcttgagta aatgcatgag tgatacgcgt
      961 tttgactctt cactgccatc ccgtatgatg attgcaagtg acaaagcatt ggccagatat
     1021 acaaatatcc aaacacccac gatggccaga aaccaccatc ccgtctgtcc gagattgtcc
     1081 acaatggttt tccatcccag tttgtaagcc atgaaaaaca aaatcgcgat tccgatgaaa
     1141 aaaaagtaat cgcgaaacac tttcattttt ttcgacatgt cgaaactttt attttaacca
     1201 agcgatttac acattttctc gtgtctgagc ctgattattt tattgactat attcaaaatg
     1261 acaataaccc acaccagata aatccatact gcattgagaa gcacagaaag aaacagaatg
     1321 ccggacaggc cattgaaagt catcatatct atcatgggca acagttggcg gcttttagtt
     1381 ctgaacgcca gacgaacatc ctcgggtata tcttcaccgt acttctcttt gagagcggcc
     1441 aggtattttt gtacttcggg agtttgtttt tcctgactaa gggtgtagat gaggtaaaat
     1501 cgggagaaga aacggtttac accagctggc atagtggcaa tgcgggctct gatgcgctcc
     1561 gacgattcaa attctttccc tttttccttg ctgataaaaa agagatgaag ggttttgtaa
     1621 taatcggtga tggcagcctg attgaggtgt gaaatagcca tcagtagagc tgcgagccag
     1681 aatagccagg tgtcgaactg tacattaagc cggtgtgcaa aagcgatata aatgagaaga
     1741 aaccaaaaat caccggcaaa tccgtccagt atacgaccaa tttcagactt aattcccgtc
     1801 aggcgggcta actggccatc cacacaatcc attatattag ctacaatcaa cgacaggatg
     1861 ccaacgacag caaatgtgag cgaataggga aaataccata aaacaccggc taccgatccc
     1921 acaaaaagtg aaagaatagt gatcatattg ggtgtaatac ccgtgccaat caatgctttt
     1981 gctattttaa atccaatggg gcggtaaaat atccggtcga gataattttc tgtttctatg
     2041 gatttaaggg aagctacgat ttctgtcttc ataagttacg tctaatgtta tacttcaaat
     2101 acttataaat aaactgacga aattttttca aatatttttg gtcaaacatc tgtcagtgac
     2161 aacgaaaata atcataatat tttgtgaacg tgcagacccg atctgtatct ttctataatt
     2221 gcagcaatga ctatcgattc tgaattcata aatttgtatt gcttccaatg cgggttattc
     2281 atctctatca gaaagtcgtt gatctcatac ctcagcccat cgccgtcaaa tttataaaag
     2341 tattttcggg tttggttcaa atcttcgtac cgcagctcga aatattcggt attccaccag
     2401 ggagcaggca cgtaggcata tccttttgtt ccgctgatta ccagatcacc ctcggttttg
     2461 acacccaggc cgatattgaa agaggctgta gcatgtttat acaatacaat acccttggta
     2521 aagaggtcaa catcattgtg tacatgtgag tagaaatgta tgtcttcata ctccattccc
     2581 agaattttga taatgggaag caatacgtac gaagccagtt cagtaatact gcccccggcc
     2641 tgagcggcat ccagttcgcg cacactgccg gttgtgagct tggagtagga aacatcaata
     2701 tcttttatat tgccgattac accgcttttc acgacggtaa tgaggtgtgc aaatgccgga
     2761 caataggctg ttttaatcgc ttctttcagt acaagcccct tatcttcggc gagtttgtaa
     2821 agttcttcgg cttctccggc cgaaagcacc atgggttttt cacacagcac atgtttgcca
     2881 tgttcgaggc ttcgccgaat atagtcgtaa tgtgtcagat gtggtgagga aatatacaca
     2941 gcatccaccc gggcaataaa atcctcatat tcagtacaat aaaaattgag ttcgttcttt
     3001 tcacaaaaac ttttcgccga ttcaattttc gggttaagaa cgcccttgac ttctattccg
     3061 cttacatatt tcgattcttt gataaaacgg ttggctatcc gtccgcttcc gatgatgcca
     3121 agctcgataa cagactgagc ttcaattctc aactgagtac tcgaaacacc ttttgtacgt
     3181 tccagatata ccacctggca gtattctttc aggtagtcga acttgccgag ccagtcggaa
     3241 ccgatggcaa aaatatcaac tccatacctt aaaatatcat caatcttttg tccttcgtat
     3301 t
//
LOCUS       DAOPZK010000186         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_39334_length_3296_cov_27.166307, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000186 DAOPZK010000000
VERSION     DAOPZK010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39334_length_3296_cov_27.166307"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKH68_08675"
     CDS             <1..684
                     /locus_tag="PKH68_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNX13050.1"
                     /translation="CSADRNIKGKINKDGIWVEKLEDNPGQYIPEEYRRQGESEAVKI
                     DLNQPMSEILKTLSQYPVSTRLSLTGTIIVGRDIAHAKLKERIDAGLGLPQYVKDHPI
                     YYAGPAKTPKGMPSGSFGPTTAGRMDSYVDLFQSQGGSMVMIAKGNRSQQVTDACHKY
                     GGFYLGSIGGPAAILAQDSIKNVEVLEYPELGMEAIWKIDVVDFPAFILVDDKGNDFF
                     QLIKTNSMC"
     gene            826..1206
                     /locus_tag="PKH68_08680"
     CDS             826..1206
                     /locus_tag="PKH68_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13051.1"
                     /translation="MQKTITMGNDEFILYIRKNYNCSLTNDRLGRMIWKWMLENSKGI
                     NKKNDIVHNVPCLWQRTIRAKKTYGIRLPRTASQITFNRNLLPKLYDYLDLLGKSYTS
                     DEPWDEWKKRDDEDENAGMLELNF"
     gene            1382..3280
                     /locus_tag="PKH68_08685"
     CDS             1382..3280
                     /locus_tag="PKH68_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX13052.1"
                     /translation="MTNYMQVENLTKSFGDNLLFENISFTITDNQRVALIAKNGAGKT
                     TLLNIIADREGYDSGNIYLKRDLRIGYLDQSPDFPKELSVIDACLQTDNDIVRTIAEY
                     EHVMLHPDKDKMNDVLARMEILKAWDYETRIKQILGKLKITDFEQKIGHLSGGQLKRV
                     ALANVLISEPDLLILDEPTNHLDLEMVEWLEEFLKRSTMSLLMVTHDRYFLDRVCTTI
                     LEIDNQALYSYSGNYSYYLEKRQERIDTFNAETESIKNIYRRELEWIRSTPQARSHKS
                     RSRTDRFKDIEERAKQNLITSNVKLGVKSSYLGSKIFEAKNICKGYENLTILDNFNYT
                     FARYEKMGIVGKNGTGKTTFLKMLLGQIKPDSGSFDIGETVKFGYYSQDGLAFDEQMK
                     VLDVVQNIAEEIDLGGGKKLSASQFLQHFLFTPETQHNYVYKLSGGEKRRLYLCTVLM
                     KNPNFLVLDEPTNDLDIVTLNILEDYLQNFKGCVIVVSHDRYFMDKVVDHLLVFRGNA
                     EIKDFPGNYTQYREEETAPQPPKGGVEDEKASKNAKKSAVANTLSSGNSRAEGKPRKM
                     SFKEKQEFEALEKEIPQLEAEKADIELQLSSGSLDNETIIKVSERYAELTDLIDEKTM
                     RWLELSEI"
BASE COUNT         1027 a          742 c          746 g          781 t
ORIGIN      
        1 tgctcggccg accgcaacat caaaggcaaa atcaacaaag acggcatttg ggtggaaaaa
       61 ctggaagata atccaggtca gtacattccc gaagaatacc gccggcaggg cgaaagtgaa
      121 gctgtaaaaa tagacctcaa ccagcccatg tccgaaattc tgaaaacgct gagccaatat
      181 ccggtttcca cccgcctctc tttgaccgga accatcatcg tgggacgcga tatagctcac
      241 gcaaaactga aagaacggat tgatgccgga cttggattgc cacagtacgt gaaagatcac
      301 cccatttact acgcaggtcc tgccaaaaca cccaagggaa tgccttcagg ctcattcgga
      361 ccaactacgg caggccgtat ggacagctac gtggatttat tccagtcgca gggcggcagc
      421 atggtgatga tagccaaagg caaccgcagt cagcaggtaa ccgatgcctg ccataaatac
      481 ggcggatttt acctcggctc catcggaggc ccggctgcca ttttggcgca ggacagcatt
      541 aaaaacgtag aagtgctgga atatcccgaa ctcggtatgg aagccatctg gaaaattgat
      601 gttgtggatt tcccggcatt tattctggtg gacgacaaag gaaatgattt ctttcagctg
      661 atcaagacga attcgatgtg ctgaaataat tgactgaaat ttgaaaaaac cgttgaaatt
      721 tttactttca acggtttttt tattggaatt cctctatcaa ttttgtagat ttgtaagcca
      781 ttgataaata aagtcttatt atccaatcat taataccttc cgaccatgca gaaaacgata
      841 accatgggta acgacgagtt tatcctctac atccgcaaga attacaactg ttcgctcacc
      901 aacgaccgcc tgggaagaat gatctggaaa tggatgttgg aaaattcgaa agggatcaac
      961 aagaaaaatg acatcgtgca caacgttccc tgtctctggc aacgaactat ccgggctaag
     1021 aaaacgtatg gtatccgcct gccgcgtacg gcttcgcaga ttactttcaa ccgaaatctg
     1081 ctgcccaaac tgtacgatta tctggatctg ctgggaaaat catacaccag cgatgaaccc
     1141 tgggatgaat ggaaaaagag agatgacgaa gatgaaaatg cgggtatgct ggaattgaat
     1201 ttctgaaata atttcaggta cagatctttt attcagtaaa ataaaaatca gggaacggga
     1261 gcacgataaa catcgtactc cttttttgtg aggattcaac ccgtcactta taatttatca
     1321 ttcataataa ataattcata tcttatccca aaaccttatc tttgtaaaaa attacattcg
     1381 tatgaccaac tatatgcagg tagaaaatct gaccaaatcg tttggcgata atctgctttt
     1441 tgaaaatata tcctttacga tcaccgacaa ccagcgggta gcactcattg ccaaaaatgg
     1501 tgcgggaaaa accacgctgc tcaacatcat agccgaccgc gaagggtacg acagcggtaa
     1561 tatctatttg aaacgtgacc tgcgcatcgg ctacctggat cagtcgcccg attttccgaa
     1621 ggaactttct gtcattgatg cctgcctgca aaccgataac gacatcgtgc gcaccattgc
     1681 cgaatacgaa cacgtgatgc ttcatcccga caaggataaa atgaacgacg tgctggcacg
     1741 catggaaata ctgaaagcct gggattatga aacccgtatc aaacaaattc tcggtaaact
     1801 gaaaatcacc gatttcgaac aaaaaatcgg acatctctcc ggcggacaac tcaaacgggt
     1861 ggcactggcc aatgtgctta tcagcgaacc cgacctgctt attctcgatg aacctaccaa
     1921 tcacctcgac cttgaaatgg tggaatggct ggaagaattt ctgaaacgct ccaccatgtc
     1981 gctgctcatg gttacgcacg accgttattt tctcgatcgc gtttgcacca ccattctcga
     2041 aattgacaat caggctcttt actcctattc cggcaattac agttattacc tggaaaaacg
     2101 gcaggagcgg attgatacct tcaatgccga aaccgaaagt ataaaaaaca tctaccgccg
     2161 cgaactggaa tggatacgca gtactccgca ggcacgctcg cacaaatcgc gcagccgtac
     2221 cgaccggttt aaggacattg aagaacgggc caaacaaaac ctgataacga gcaacgtaaa
     2281 actcggtgta aaatcaagtt acctcggttc caaaattttc gaagcgaaaa atatctgcaa
     2341 aggttacgag aacctgacca ttctggataa tttcaattac acttttgccc gctacgaaaa
     2401 aatgggcatc gtcgggaaaa acggaacggg aaaaaccact tttctgaaaa tgcttctggg
     2461 gcagataaaa cccgacagcg gcagttttga cataggtgaa acggttaagt tcggttatta
     2521 cagtcaggat gggctggcgt tcgatgaaca gatgaaagtg ctggatgtag tgcagaatat
     2581 tgccgaagaa attgatttgg gaggcggtaa aaagctttcg gcttcgcagt ttctgcaaca
     2641 tttccttttt actcccgaaa cgcagcacaa ttacgtgtac aaactcagcg gcggcgaaaa
     2701 acggcgcctg tatctttgca ccgtgctgat gaaaaatccg aattttctgg ttcttgatga
     2761 acctaccaat gatctggata tcgttacact gaacattttg gaagattacc tgcagaattt
     2821 caaaggctgc gtgattgtgg tgagccacga ccgctatttt atggataaag tggtggatca
     2881 tctgcttgtt ttccgcggaa atgcggagat taaggatttt ccgggaaatt atacgcagta
     2941 cagggaggaa gaaacagccc cccaaccccc taaaggggga gttgaagacg agaaagcttc
     3001 aaagaatgcc aaaaaatctg cggttgccaa taccctttct tccggcaata gtcgggctga
     3061 gggtaagcca cgcaaaatga gtttcaaaga aaagcaggaa tttgaagcgc tggaaaagga
     3121 aataccgcaa ctggaagctg aaaaagcgga tattgaactt caactatcgt cgggctcgct
     3181 ggataatgaa actataataa aagtttccga acgctatgcc gaactgactg atctgataga
     3241 tgaaaaaacg atgcggtggc tcgaactgag cgagatttaa caggaaagtt agtgaa
//
LOCUS       DAOPZK010000187         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_39506_length_3286_cov_22.602290, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000187 DAOPZK010000000
VERSION     DAOPZK010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39506_length_3286_cov_22.602290"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /gene="ispF"
                     /locus_tag="PKH68_08690"
     CDS             <1..446
                     /gene="ispF"
                     /locus_tag="PKH68_08690"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNX13053.1"
                     /translation="FAPDRELWLGGVKIEHTVGLLGHSDADVLIHALCDALLGAASLR
                     DIGYHFPDTAGEYKNIDSKLLLMETMILLREKGYEFENADCTVCAEEPKLNPHIPQMQ
                     QTLAGVMGVDEDDISIKATTSEKMGFVGRREGIAAYVSVLISKQK"
     gene            558..1184
                     /locus_tag="PKH68_08695"
     CDS             558..1184
                     /locus_tag="PKH68_08695"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX13054.1"
                     /translation="MSVLVGKKAPAVTGKAVVNGNDIVDNFSLEQYRGKYVVFFFYPA
                     DFTFVCPTELIAFQEKIEEFTSRNAVLVGASTDSEFSHWKWLQTPQNAGGIQGVKYPL
                     FADHNLMVSKAYDVLAGYEDYTEEGEPIFVGEAQAYRGLFLIDKEGIVRHQVVNDMPL
                     GRSVEEVIRVLDALQFTEEYGEVCPANWKKGDKALKATQEGISDYLSH"
     gene            1342..2097
                     /locus_tag="PKH68_08700"
     CDS             1342..2097
                     /locus_tag="PKH68_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNX13055.1"
                     /translation="MTWLYALVSTLIVSLISLTGVFLLSFKTQQLHRVLILLVCFSIG
                     AMLGNSFFELLPESFLHNPNTTLTGWLVMGGFLVFFILEQFLHMHQHTNFDQQKSVKT
                     YGYLSLYADSIHNITDGILIGAAWMFTPEIGIATTVAILLHEIPQEISDFGILLQAGF
                     SKRKALLFNFYAACTAILGTVVTLAVGEKVEHLSIYILPVAAGGFIYLAAASLLPEIL
                     KETNKRNFLVHLSFILLGLIFMYLFSIHGGHQH"
     gene            2196..>3286
                     /locus_tag="PKH68_08705"
     CDS             2196..>3286
                     /locus_tag="PKH68_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13056.1"
                     /translation="MKKHLIMILFLVAGLPVTAFGQELQWNLNLNYFFDNTEFAPSTL
                     SADQTMAGVHFAPEIGLKWEGNHSVYAGVDLMKKSASKSVLDKARLIAYYQYKNENTL
                     FKAGAFPRGELLSDYSTAFFQDSIGYYRPVMEGLFLKMGNDKGFVKLWMDWTGLQTNV
                     DRETFYLGASAYKQIWGPFFADFQSYMFHFAGTNPINPYLSVCDNALGQLSVGVKYSN
                     IWVLNNLMASVGVLSGFERDRYDINNYGTPTGAIIRLNADYKGIGTENNLYFGQPHMV
                     FYRRYGNELYWGNPFYREDRYIESKLYWNVFETQTVRGQLAAKFHYTEGRVLSEQLFT
                     LRASIGSDQPRKRKMMIWDLFNNKKANSLR"
BASE COUNT          946 a          630 c          722 g          988 t
ORIGIN      
        1 catttgctcc cgaccgcgag ttatggcttg gcggggtgaa gattgaacac accgttggtc
       61 tgttggggca ttctgatgcc gatgtgctta ttcatgcact atgcgatgca ctgctcggtg
      121 cagccagtct gcgcgatatc ggctaccatt tccccgatac agccggtgaa tacaaaaaca
      181 ttgacagcaa attgctgctg atggaaacga tgattcttct gcgtgaaaaa gggtacgaat
      241 ttgaaaatgc cgattgtacc gtatgtgctg aagagccaaa actcaatcct cacatacctc
      301 aaatgcagca aacactggcc ggcgtgatgg gcgtggatga agatgatatt tcgattaaag
      361 ccaccacttc cgaaaaaatg gggtttgtag gccggcgtga aggaattgcc gcttatgttt
      421 cggtacttat cagtaaacaa aaatagcact tcaagcgttg taaagtttca atttcatttt
      481 cacagactcc atttatcaga aaattagcgg taaagtgaaa ttggaacaaa cataaaaaac
      541 aaattaaatt aaagaaaatg tcagtattag taggaaaaaa agcaccggcg gtgaccggta
      601 aagccgtagt aaacggcaat gatatcgttg ataatttttc actcgagcag tacaggggaa
      661 aatatgtagt atttttcttt tatcctgcgg actttacctt tgtatgtccc actgaattaa
      721 ttgcatttca ggaaaaaata gaagaattta cctctcgcaa tgccgtattg gtaggagctt
      781 ccaccgattc ggaattttca cactggaaat ggcttcaaac cccgcagaat gcaggtggaa
      841 ttcagggtgt gaaatatcct ctttttgccg atcacaatct gatggtctcc aaagcgtatg
      901 atgtgttggc cggatatgaa gattatactg aggaaggaga acctattttt gtgggtgaag
      961 ctcaggctta tcgtggattg ttcctgatcg acaaagaagg catcgttcgt catcaggtgg
     1021 tgaatgacat gccactcgga cgcagtgtgg aagaggtaat ccgtgtactc gatgcgctgc
     1081 agtttaccga agaatacggt gaagtatgtc ccgccaactg gaaaaagggc gataaagcac
     1141 tcaaagcaac tcaggaagga atttcagatt atctctcgca ttaacagatt aatttgaatt
     1201 tatacaaaaa agactgtttc agataaacct tagacagtct ttttttgtgt acatttgcac
     1261 aagtcggata attttataaa atttgtagtt gaaaacaaag gataaaaaat attttttcct
     1321 tttaataata ttttaatgga aatgacatgg ctatatgcct tagtaagcac cctgattgtt
     1381 agtttgattt cgttgaccgg tgtttttttg ttatcattca agacacagca gcttcatcgg
     1441 gtattgatat tgctggtatg cttttcgata ggagctatgc tgggaaattc tttttttgag
     1501 ctgctgcccg aatcttttct tcataatccc aatactacgt taaccggatg gttggtgatg
     1561 ggcggatttt tggttttttt tatactcgag cagtttctgc acatgcacca gcataccaac
     1621 ttcgaccaac aaaaatcggt aaaaacctat ggttatttaa gtctgtatgc agattcaata
     1681 cacaatatta ccgatggaat tttaatcgga gctgcttgga tgtttactcc cgaaatagga
     1741 attgcaacca ctgtagccat ccttttacat gaaattccac aggaaatcag tgattttggt
     1801 atattgctgc aggccggttt ctccaagcgt aaggccttgt tgttcaattt ttatgctgcc
     1861 tgtacagcca ttttgggaac tgtagttacc ctcgctgtgg gcgaaaaagt ggaacatctt
     1921 tccatttata tacttccggt agcggctggc ggatttattt atcttgctgc agcaagcctg
     1981 ctgcccgaaa ttttgaaaga aaccaacaaa cgaaattttt tagtacattt atcctttata
     2041 ttactgggat tgatattcat gtatttattc agtattcacg gaggacatca acattaattt
     2101 gcgtatgaaa cgagcatgaa tacatatatt caccgaagta gatgaaaatc tgacatgcga
     2161 gttccatgtg acctttaaaa aacaattatt tacacatgaa aaaacatctg ataatgatcc
     2221 ttttcctcgt tgccggattg cccgtgactg ctttcggcca ggagcttcag tggaatttaa
     2281 atctcaatta ttttttcgat aatacagaat ttgcaccttc cactttgtcg gccgaccaaa
     2341 ccatggcggg tgtgcatttt gcacctgaaa taggtctgaa atgggaggga aatcattcgg
     2401 tttatgctgg tgtggatttg atgaaaaagt cggcttccaa atcagtgctt gacaaagcaa
     2461 ggctcatcgc ctattatcag tataaaaatg aaaatacgct gtttaaagcc ggtgcttttc
     2521 ccaggggcga actcctatcg gattattcta cggcattttt tcaggattca attggatatt
     2581 accgccctgt gatggaagga ttattcctga aaatgggaaa tgacaaggga tttgtgaaat
     2641 tgtggatgga ctggaccgga ttacagacta atgtggatcg cgaaacattt tatttgggag
     2701 catcagctta caagcagata tggggacctt ttttcgctga ttttcagtcg tatatgtttc
     2761 attttgccgg tacgaaccca attaatccct atttatcggt ctgtgataat gcgctgggtc
     2821 agttatcggt gggagtgaag tattccaaca tttgggtact caacaattta atggcttctg
     2881 ttggagtttt gtcaggattt gaacgtgaca gatacgatat aaacaactac ggtaccccta
     2941 ctggtgccat catacggctg aatgccgact acaagggcat tggaaccgaa aacaatttat
     3001 acttcggtca acctcacatg gtattttatc gccgctatgg caatgagtta tattggggta
     3061 atccctttta ccgtgaagac cgttatattg aaagcaaact ttactggaat gtgtttgaaa
     3121 cacaaacagt ccggggtcag ctcgctgcca aatttcatta cacagaaggt cgggtgctct
     3181 ccgaacaact ctttaccctg cgtgcttcca taggtagcga tcagccccgc aagcggaaaa
     3241 tgatgatttg ggatttgttt aataataaaa aagctaactc cttacg
//
LOCUS       DAOPZK010000188         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_39768_length_3271_cov_20.215796, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000188 DAOPZK010000000
VERSION     DAOPZK010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39768_length_3271_cov_20.215796"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="PKH68_08710"
     CDS             complement(<1..1517)
                     /locus_tag="PKH68_08710"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNX13057.1"
                     /translation="MQVQFRLNYQALWGQSIYLQVYTGDEQYVRHEMICKGVNLWLVK
                     INLDENATEIQYRYAIRNQDKTFENEFGLMRRLKIPKTDKIIALEDYWRIPNGDSAFN
                     TLAFTDCFFNRKNAPKSKPSVNINFILRINCPQMEPDRHFAIIGNQEALGNWDVSKKV
                     RLDETLYPFWSIALNTHDIKFPLEYKYLIVDTKSDEVLAWGGGPNRTVLNADEEKLTI
                     VTDENFIRTIPSWKTAGVAIPVFSLRSEEGFGIGEFLDLKKMVDWAKLTGQRIIQTLP
                     INDTILNHTNYDSYPYNAVSVYALHPIYLRLDRLGVPKYKKDIVYFENKKKELNENSF
                     SDYQNVLNEKWKYFQLIYPKESPAVFASDDYRQFFESNKTWLVPYAVFSYLRDKNETP
                     EFGKWGEFSTYNADEIVAFADEGNPQYPEIALFYFLQYHLHKQLIEVHEYARSKGVAL
                     KSDIPIGVSPRSVDAWVEPQLFNTNMQAGAPPDDFSVTGQNWGFPTYNWDEMAKDG"
     gene            complement(1662..1732)
                     /locus_tag="PKH68_08715"
     tRNA            complement(1662..1732)
                     /locus_tag="PKH68_08715"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1698..1700),aa:Cys,seq:gca)
     gene            complement(1887..2363)
                     /locus_tag="PKH68_08720"
     CDS             complement(1887..2363)
                     /locus_tag="PKH68_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13058.1"
                     /translation="MRNLKYIVLSTLMVLTLTAQAQRERHGHPNVKEMHERKWQFVMN
                     EVSLSPAEMNAVKPVFLDYEKKMWELHMKNREYFRMGVEKKQDAAINYAELNDKAVNS
                     EISQAQLLRAYHMKLRKLLKPETLYYYYQAERKFKRKLLQNMPKNMPPPPDEELKR"
     gene            complement(2378..2704)
                     /locus_tag="PKH68_08725"
     CDS             complement(2378..2704)
                     /locus_tag="PKH68_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13059.1"
                     /translation="MMEKKNISLDKIDKKLPFSVPENYFEDFAARMELQVAGETLQPP
                     HRHILNWRVAVAAVFAGILLAGAIYTTIFKNASKSDNYESYVLSQVDESSMLYYYVGE
                     PTTETK"
     gene            complement(2757..>3271)
                     /locus_tag="PKH68_08730"
     CDS             complement(2757..>3271)
                     /locus_tag="PKH68_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNX13060.1"
                     /translation="HKDKATAFSGIVRTYQERLYWHIRKMVLSHDDANDILQDVFMKA
                     WNGLDNFRGDSQVSTWLYRIATNETLTFLAAKRMKNINSLDEVEDVLLQNLKADSYFN
                     GDETQLQLQKAILALPEKQRLVFNMRFYDDLKYEEMSEVLGTSVGALKASYHHATKKI
                     EQILTGKIED"
BASE COUNT          844 a          696 c          673 g         1058 t
ORIGIN      
        1 ccatctttgg ccatttcatc ccagttgtac gtgggaaaac cccagttttg ccccgtcacc
       61 gaaaaatcat cgggcggtgc gccggcctgc atattggtgt tgaacaactg cggctccacc
      121 caggcatcca cactgcgggg acttactcct attggaatat cacttttgag tgcaactcct
      181 ttgctgcggg cgtattcatg aacttctatc agttgtttgt gcaaatgata ttgcagaaaa
      241 taaaacaggg caatttccgg atattgtgga ttaccttcat cagcaaaagc cactatctca
      301 tcggcattgt aggtactgaa ttcaccccat ttcccaaatt cgggtgtttc attcttatcc
      361 cggaggtacg aaaaaaccgc atacggcacc agccaggttt tattggattc gaaaaattga
      421 cggtaatcat ccgaagcaaa tacagccgga ctttctttag gatatattaa ttgaaaatac
      481 ttccattttt cgttcaaaac gttctgataa tccgaaaaac tgttctcgtt cagttctttc
      541 tttttgtttt caaaataaac aatatccttt ttgtacttcg gcacacccaa tcggtccagc
      601 cgcaggtaaa tgggatgcaa cgcatagacc gaaacggcat tatagggata cgaatcgtaa
      661 ttggtgtgat tcagtatggt gtcgttgatg gggagtgttt gaattatacg ctgtccggtg
      721 agtttagccc agtccaccat ttttttcaga tcgagaaact caccgattcc gaaaccttct
      781 tcacttcgga gtgagaatac gggaatggcc actcctgcgg ttttccacga gggtatcgtc
      841 cggatgaaat tttcatcggt gacgatggtt aatttttcct catcagcatt gagtactgtt
      901 cggttgggtc cgccacccca ggcgagcact tcatcggatt tggtatccac tatcaggtat
      961 ttgtattcca gtggaaattt tatatcatgg gtattgagag ctattgacca gaaaggatat
     1021 aaagtttcat ccaacctcac ttttttgctt acatcccagt ttcccagggc ttcctgattt
     1081 ccaataatgg caaaatgccg gtcgggctcc atttgggggc aatttatccg caggatgaaa
     1141 ttgatattaa cggatggttt acttttggga gcattttttc gattaaaaaa gcagtcggta
     1201 aatgccagtg tattgaaggc actgtcgcca tttgggatgc gccagtaatc ttccaaagca
     1261 attatcttat ccgttttggg gatttttaat ctccgcatga ggccaaactc attttcaaac
     1321 gttttatcct gatttcggat agcgtagcgg tattgtatct ctgtggcgtt ttcgtccaga
     1381 tttattttga ccaaccataa attaacgcct ttacatatca tctcgtggcg aacgtattgt
     1441 tcgtcgccgg tatagacctg taagtaaatg gattgtcccc agagagcctg ataattgagt
     1501 ctgaattgta cctgcatagg tgtaaagtat tattgtgtgc aaatttacaa attaataaac
     1561 ctgctcaaaa gaaataacga attgcctaac taaatgagag ctaaaacgtt tgcgataaaa
     1621 aacaccctct ataaatttta ttttatagag ggcgtctgat gaggtacttg gcggattcga
     1681 accgccgtaa acggttttgc agaccggtgc ctagccactc ggccaaagta ccctggtttt
     1741 ggactgcaaa gatagtatta ttttgtcagt tgacaaaatt tcatttcggt tttttcgata
     1801 gcccgttgat acaaccattt aaaaaaagaa cacaacacaa aaaatcaatt gtgttgtgtt
     1861 tttttgtaca ttcttatagg aagattttac cttttcaatt cttcatcagg aggtggaggc
     1921 atgtttttcg gcatattctg cagcagtttt cgtttgaatt ttctctctgc ctgatagtaa
     1981 taataaagag tctcgggttt gagtaatttg cgcagcttca tgtgatatgc cctgagtagc
     2041 tgcgcctgcg atatttctga atttaccgct ttatcattaa gttcagcata gttgatagca
     2101 gcatcttgtt ttttctctac ccccatccga aagtactccc tgtttttcat gtgaagttcc
     2161 cacatttttt tttcataatc cagaaatacc ggttttacgg cattcatttc ggcaggcgaa
     2221 agcgaaactt cattcatcac aaactgccat tttctttcgt gcatctcttt cacattcggg
     2281 tgtccatgtc gttcccgctg tgcctgagct gtcaacgtga gcaccatcaa tgtgcttaaa
     2341 acgatatact ttagatttct cattggtcag gattttttta tttggtttct gtagttggtt
     2401 cacctacata ataatacagc atggaggact cgtccacctg cgacaataca taggattcat
     2461 aattatcaga ttttgaagcg tttttaaata ttgtggtata tatagcgcct gccagaagta
     2521 ttcccgcaaa aacagcagcc actgcaaccc tccagttcaa tatgtgacgg tgaggtggtt
     2581 gcagtgtttc accagcgacc tgcaattcca tgcgagctgc aaaatcctca aaatagtttt
     2641 cgggaaccga gaatggtaac ttcttatcta ttttatccag cgatatgttt ttcttttcca
     2701 tcatgttgtt ttgactgttt ttagcataaa aggtttaaat atctcattct taaaacttag
     2761 tcttcaattt ttccggttag aatttgctca attttcttag tggcatgatg ataggatgct
     2821 ttcagtgcac ctacggaggt tccgagcacc tcgctcattt cttcatattt caagtcgtcg
     2881 taaaatcgca tattaaatac caatcgttgt ttttcgggca gggcgaggat ggctttttgc
     2941 agctgcaact gggtttcgtc gccgttaaag taagaatcgg cctttagatt ttgcagtaac
     3001 acatcttcca cttcatccag cgaattgata tttttcattc ttttggcggc caaaaaagtg
     3061 agggtttcgt tggtggctat ccggtaaagc caggtagata cctgcgaatc accccgaaaa
     3121 ttatcgagtc cgttccaggc tttcatgaat acatcctgca gtatatcatt ggcatcatcg
     3181 tgcgaaagca ccattttccg tatgtgccag tacaatcgct cctgataggt gcgtacaatg
     3241 cccgaaaatg cagtcgcttt gtctttgtgg g
//
LOCUS       DAOPZK010000189         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_39779_length_3270_cov_23.050078, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000189 DAOPZK010000000
VERSION     DAOPZK010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39779_length_3270_cov_23.050078"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1167)
                     /locus_tag="PKH68_08735"
     CDS             complement(52..1167)
                     /locus_tag="PKH68_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13061.1"
                     /translation="MITIQEAKTKKEMKQFIMFPFELYKNDKTWVPPLIFDEKNTFNP
                     RKNPSFEFCEAAFFLAFKDNKVVGRIAAIINHKANETWNEKRTRFGWIAFIDDPDVSK
                     ALLDTAEKWGADKGMTGIHGPLGFTDLDPEGMQVEGFENNATITSAHNYPYFPVHMEK
                     HGFRKSVDWVQYKFNASQPIPEKVARINKLIAQKYKVNTVVPKTKKEAMKYVPGFFKG
                     VNEGFKNLYGYSQLSDKQIKYYTDAYFGFARPELLCFLTDENDEVVGFGICFPSLSKA
                     FQKAKGRLFPFGFIHILKALRNYDEIDLYFNGVIPEWQNKGIHSLYYVSMNEAAIARG
                     VKWAYSTGQLETNVNAVGIWDNYDKVPLFRTRCYIKE"
     gene            1532..>3270
                     /locus_tag="PKH68_08740"
     CDS             1532..>3270
                     /locus_tag="PKH68_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNX13062.1"
                     /translation="MKKRIFILSLMISLITGLQAGVEHILPKPQQITTNASGSFNLAQ
                     SLRLILPTINSNDPAVNTELTNLITTNGGTVSESATNTIEVQLVASIAGAEFQKEAYS
                     LNVTPEKITISATTLQGAYWATQTLWQLADNAGGNLTACSIIDWPAFSIRGYMQDIGR
                     SYMEFDEIKKEIANLSRYKINVFHWHLTENQGWRLQSKLFPQLNANNSTAYTRHPGKF
                     YTMAQARELVQWCSKHGVLLIPEMDMPGHSEAFTRVFGYNMQTTQGLATMKQLITEAI
                     DSCFSTSPILHIGTDEVTMTMPNFVPEMVTLIRAKGLKIGTWSKGYTGYNAADIGLTQ
                     LWASSGTIIPGTPNVDSRYHYMNHFDDYADVVGMYNSSILEVTKQTAERVGVIMAVWN
                     DRKTRTDRDIVIQNGVYPAVLATAERAWLGGGTYITTKGVMLDAKNTETFRNFADWER
                     RFLYHKANHLKNEPIAYVKQTNVNWRITDAFPNGGTLTTSFPPETQLADTYTYNGNTY
                     GTRKATGAGIYLRHVWGTIIPTFYSSPQTNSTAYAYTWVYSPIAQTVGANIEFQNYGR
                     SESDVPPRQGTWD"
BASE COUNT          951 a          729 c          680 g          910 t
ORIGIN      
        1 aaaaaaatgc ccaggttcaa attgggcatt tttgattgta taatttgtat tttattcttt
       61 gatgtaacag cgggtgcgga ataacggaac tttatcgtaa ttgtcccaga taccaacggc
      121 attcacattc gtttcgagct gaccggtgga ataagcccat ttaactccac gggctattgc
      181 cgcttcgttc atggatacat aatagagtga atgtatgcct ttgttttgcc attcggggat
      241 tacaccgttg aagtagagat ctatctcatc gtaatttctc aatgctttca gaatgtgaat
      301 aaatccgaag gggaataacc ggccttttgc tttctggaat gctttggaca gcgagggaaa
      361 acagatgcca aaaccaacca cttcgtcgtt ttcgtcggtc aggaagcaga gtaattcggg
      421 tctggcaaat ccaaagtaag catcagtgta gtattttatc tgtttgtcgg aaagctggct
      481 gtagccgtac agatttttga agccttcatt cactcccttg aaaaatccgg gaacatattt
      541 catcgcctct tttttagtct ttggtacaac ggtgtttacc ttgtatttct gagcaatgag
      601 tttgttgata cgtgccactt tttcggggat gggctgtgaa gcgttgaatt tatactgcac
      661 ccagtccacc gatttgcgaa atccgtgttt ttccatgtgt accggaaagt aaggataatt
      721 atgtgcactg gtgatggtgg cattgttttc gaaaccttcc acctgcattc cttccggatc
      781 gagatcggtg aaccccagtg gaccatgaat gcccgtcata cccttatcgg caccccattt
      841 ttcggcagta tccagcagcg ctttcgacac gtccgggtcg tcgataaagg caatccaccc
      901 aaaacgggtg cgtttttcgt tccaggtttc gttggctttg tgattgatga tggcagcaat
      961 tcgtcccacc actttattat ccttgaaagc cagaaaaaag gcagcttcac aaaattcaaa
     1021 ggaagggttt ttcctggggt taaacgtatt tttttcgtca aaaatcaaag gaggaaccca
     1081 tgttttgtca tttttataaa gctcgaatgg aaacatgatg aattgcttca tctctttttt
     1141 ggttttagct tcctgtatgg taatcattga atatgtgtta ttttttagat ttcaacctgc
     1201 aaatatacaa tttttttaca aaagcattac aattatatta atacaacggc gaatcgcatg
     1261 acgaaagata ttatccggag aatccctttc aatatatttc cggataaaat gagaaaattg
     1321 aaatagttta aaagtcatcc cgatattcac tcccttcaac ctttctgctc ctctcaatac
     1381 ctcacctaat cccttgattt cgaaaaaaaa agaaaattga aattatttaa tttaggtttg
     1441 ttagcattct gattggtaga gaataattat ctttgtgcca ttaaaaaagt caaaaaaatc
     1501 acaaatcctt tttttaataa aatctaatgc tatgaaaaag cgaattttta ttttatccct
     1561 gatgatctcc ctgattacag gattacaagc tggtgtggaa cacattctcc caaaaccgca
     1621 gcaaataaca accaatgcca gtggctcatt taatctggca caaagcctgc ggttaattct
     1681 tcccacaatc aacagtaatg atccggcagt aaacacagag ctaactaatc tgattaccac
     1741 caatggaggt acggtttctg aaagtgccac caatactata gaggtacagt tagtggcttc
     1801 gattgcaggt gccgaatttc agaaggaagc atactcactc aatgtaacac ccgaaaaaat
     1861 aactatcagt gccaccacgc tccagggagc ttactgggct acccaaaccc tgtggcaact
     1921 tgccgacaat gccggaggca atcttactgc ctgcagcatt atagactggc cggcattcag
     1981 cattcgtgga tacatgcagg atattggccg cagctatatg gagtttgatg aaataaaaaa
     2041 agaaattgct aatctttcac gctacaaaat caatgttttt cactggcacc taaccgaaaa
     2101 ccagggctgg cgtttgcaaa gcaaattatt tcctcagttg aatgccaata acagtacggc
     2161 ttatacccgt catccgggaa aattctacac catggctcag gcacgggaat tagttcaatg
     2221 gtgctcaaaa cacggggtgc tgcttattcc tgaaatggac atgcccggcc acagcgaagc
     2281 atttacccgc gtttttggat ataatatgca aactactcag gggctggcaa ccatgaagca
     2341 actgattacc gaggcaatcg actcctgttt ttccacctca ccaattctac atattggaac
     2401 cgatgaggta actatgacca tgcctaattt cgttcccgag atggtaacac taattcgtgc
     2461 aaaaggctta aaaattggca cctggagcaa gggttacacc ggttacaacg ctgcagatat
     2521 aggcctgacg cagttatggg cttcttcagg aaccattatt cccggaactc caaacgtgga
     2581 tagccgctac cattatatga atcacttcga cgattatgct gacgtggttg gaatgtacaa
     2641 cagcagcata ctcgaagtaa ccaaacagac cgccgaacgt gtcggggtga ttatggctgt
     2701 ttggaacgac cggaaaacca gaactgaccg ggacatcgtc attcaaaacg gcgtttatcc
     2761 agccgtattg gctactgccg aaagggcctg gctgggcggt ggaacctaca ttacaaccaa
     2821 aggagttatg ctggatgcaa aaaatacgga gacattccgg aattttgcag actgggaacg
     2881 tcgtttcctg tatcacaaag ccaatcacct gaaaaacgag ccaattgcct atgtgaaaca
     2941 aaccaatgtt aattggcgta ttaccgatgc attcccaaat ggaggaacgc tgaccacctc
     3001 ctttccacca gaaacccaac tggcagatac ttacacttac aacggtaata cctatggtac
     3061 acgcaaagct accggagctg gaatctacct gcgccacgtg tggggaacaa tcattccgac
     3121 attttattcc agtccgcaga ctaattcaac ggcttatgct tatacctggg tatattctcc
     3181 catagcacaa acagtagggg caaatattga attccagaat tatgggcgtt ctgaatcgga
     3241 tgtgccacct cgacagggta cctgggacta
//
LOCUS       DAOPZK010000190         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_40382_length_3238_cov_26.142319, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000190 DAOPZK010000000
VERSION     DAOPZK010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40382_length_3238_cov_26.142319"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..679)
                     /gene="idi"
                     /locus_tag="PKH68_08745"
     CDS             complement(158..679)
                     /gene="idi"
                     /locus_tag="PKH68_08745"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNX13063.1"
                     /translation="MEDQVILVDVHDAPIGLTGKLEAHQKALLHRAVSVFIFNSQQQL
                     LLQKRAADKYHSACLWTNTVCTHPKPGESNSDAVRRRLKEEMGLDVQEVTKIFDFTYL
                     EKLGNELTEHEFDHVFIGFSDDLPLPNADEVSDFRFVDVDLLRNEIKTNPQNYTVWFR
                     KIIDRVVSEMNFR"
     gene            complement(680..1522)
                     /locus_tag="PKH68_08750"
     CDS             complement(680..1522)
                     /locus_tag="PKH68_08750"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNX13064.1"
                     /translation="MSVAFYIENSYKVSELITKKYSTSFSLATSVLEKEKRKAIYAIY
                     GFVRLADEIVDSLHNYDKSFLLEKLTEDLEYALKYGISTNLILASFVDTAKKYRIKKE
                     HIDAFMQSMEYDLTKINYTETSDLNNYIYGSADVVGLMCLTVFCGGNQTLYDELEHPA
                     QKLGSAFQKVNFIRDLREDTGDLGRNYFPEISVDKFSKETKSLIEKSIQQDFDEAFTG
                     IKRLPGRSKLAVALAYYYYLSLFKKIKKTSPEKILSMRVRISNFIKYLIIIRVSILYK
                     LKFI"
     gene            complement(1949..>3238)
                     /locus_tag="PKH68_08755"
     CDS             complement(1949..>3238)
                     /locus_tag="PKH68_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNX13065.1"
                     /translation="DLLNIKYGHARIENCIFEGNAEYDSDAIDYDGIENGIIRNTKIL
                     NTLGNNGDAIDIGEESHNIIIDSVLIINAYDKGVSIGQRSSVKLTNSLLLNCNIGAAV
                     KDSSRIYIDKCTFYGNGSSVSCYEKNPGRAGGNARVKNSILSNAYLASYQSDSKSTIA
                     FSNCISDNDLLPVETSNLFGNSLFRNPTLFDFSLSAGSPARLTGSQNGLKTDMGTYFS
                     TIAFQPEVLICGIYLNPLNTLQPEYLALYNPSLNTVDLSDYRIDKGVDCSFPQGTLLA
                     PGDTLYVTADASGWRSDYQVVQWNSGKLSNEGEAVQLLNKYGLVADYLKYSAQEGWPV
                     AAFNSDFYLSLNSPYLDNHFGQNWKAKSLTTGLKGVNGNENFKIQVYPNPAADWLTLK
                     GKFETNTPVALYTISGQKIKETTADRSGYTVMAVSLI"
BASE COUNT          942 a          643 c          601 g         1052 t
ORIGIN      
        1 aaatctaata tatatcaaga atttagaatt ttaccggaaa atagtaaaaa taaattattt
       61 agtttaatga aaagtcaaga tgacttctga ataatgcgaa aaacataacc atcggtcaca
      121 tttgaaaaaa tcatttattc tacctcatat caatatttta cctgaaattc atttcactca
      181 ccacacggtc aatgattttg cgaaaccaaa ccgtgtagtt ttgagggttg gtttttattt
      241 cattcctgag taagtccaca tccacaaacc tgaagtccga cacttcgtcc gcattgggca
      301 aaggcaagtc atccgaaaat ccaataaaaa catgatcgaa ttcatgctcg gtaagttcat
      361 tccccagttt ttccaggtag gtgaaatcaa aaattttggt tacttcttgt acgtccagtc
      421 ccatttcttc tttcagtctt cttcttactg catccgaatt gctttcgccg ggtttagggt
      481 gtgtacacac cgtattggtc cacaagcagg ccgaatgata tttatctgcc gctcttttct
      541 ggaggagcag ctgctgttgg gaattaaaaa taaacaccga tacagcccgg tgaagcaagg
      601 ctttttggtg tgcttccaat tttcccgtta acccgatggg ggcatcatgt acatctacta
      661 atattacctg atcttccatt taaatgaatt tgagtttata cagaatgctt actctgataa
      721 ttatcaggta tttaatgaag tttgaaatcc gtacccgcat cgagagtatt ttttcgggtg
      781 aagttttctt tatttttttg aataatgaaa ggtaataata atatgccagc gcaacagcca
      841 gtttcgatcg gcccggtaat cgtttgatcc ctgtaaatgc ttcatcaaaa tcctgttgga
      901 tagatttttc aataagtgat ttcgtttcct tcgaaaattt atcaactgaa atttcaggga
      961 agtagttgcg gccaaggtct ccggtatctt ccctcaggtc gcggatgaag ttaactttct
     1021 gaaaagctga gcccaatttt tgagccggat gttccagctc gtcatacaag gtttgatttc
     1081 caccgcaaaa tacggtgaga cacattagac ccactacgtc ggccgaaccg taaatgtaat
     1141 tattcagatc ggaggtttcg gtataattta ttttcgtaag gtcgtactcc atactttgca
     1201 taaatgcatc gatatgttcc tttttaattc tgtatttttt tgccgtatcc acaaacgagg
     1261 ccagaatcag attggtgctt atcccatatt tcagggcata ttccagatct tcggtgagtt
     1321 tttcgagcag gaaggacttg tcataattgt gtaaactatc cactatttca tcggccagcc
     1381 ttacaaatcc ataaatggca taaatggctt ttcttttttc cttttcaagt acggaagtag
     1441 ccagcgaaaa agaggtgctg tactttttag tgataagttc agatacttta taactatttt
     1501 cgatgtaaaa agcgacactc ataggtggtt tgttttgtca aattccaaca actttattag
     1561 aaaaagtatt caatgggtaa atatattata tttttagtgg ttttcaataa ttagatattc
     1621 ttatttttca cttgtttaac tactcaaaga gatgtaaagg tcatgaaaca ataaatcact
     1681 aaaaaaacaa atgtaaaatc tttgtttaga cgattaaaat cattttagaa tattgtacct
     1741 gataacagtc agcatgtcag atatataatg gttgtgagcc tgatgtgaaa ttaagaggag
     1801 acatactacc ttcttctaac tatttgtcag acttgcggtg aaaaggtttg ctctgaaacc
     1861 aacgtttaac gtgcctgatg tttctacttt ccggcaatcg ttacttttac agtttgcgaa
     1921 cccgctttga taatataaat tcctgccttc agatcagaga aacagccata actgtataac
     1981 cgctcctgtc tgctgttgtt tcttttatct tttgtccgga tattgtataa agtgcaaccg
     2041 gagtattggt ttcaaatttt cctttcaacg ttaaccagtc tgcagccgga ttaggataaa
     2101 cctgaatttt gaaattctca ttcccattta cacctttcag tcccgttgtt aatgattttg
     2161 ctttccagtt ctgtccgaaa tggttgtcta aatacggact attcagagat aaataaaaat
     2221 cagagttaaa tgcagcgaca ggccatcctt cttgcgctga atatttaaga tagtcggcta
     2281 ccaacccgta tttattcagt aactggacgg cttcaccttc gttggacaac ttgcctgaat
     2341 tccactgaac gacctgataa tctgatctcc agcctgatgc atcggcagta acatacagag
     2401 tatcacccgg agcaagcagc gttccctgtg gaaagctgca atccactcct ttgtcaatcc
     2461 tgtaatccga caaatcgaca gtatttaacg aaggattata aagtgccaga tattcgggct
     2521 gtaatgtatt taaaggattc aggtagatgc cacaaatcaa tacctcgggt tgaaatgcta
     2581 tagttgaaaa atatgttccc atatccgttt tgagaccatt ctgacttcct gttaatcgtg
     2641 ccggtgaacc ggccgagagt gaaaagtcaa acaaggtggg attacggaat aaagaatttc
     2701 caaaaagatt ggaagtttca accggcaaca gatcattatc cgaaatgcaa tttgagaatg
     2761 caatggtgga tttactatcc gactgataac ttgccagata tgcatttgac aaaatgctgt
     2821 tttttaccct tgcattgcct ccggcacgtc cgggattctt ttcatagcaa ctcaccgaag
     2881 atccattgcc gtagaaggta catttatcaa tatagattcg gcttgaatct tttactgcag
     2941 cacctatgtt gcagtttagt aacagactgt tggtgagctt gaccgacgac cgctgaccga
     3001 tagatacccc tttgtcgtaa gcatttatta tcaatacgct gtctattata atattgtgcg
     3061 attcttctcc gatatctatg gcatcaccgt tgttgccaag ggtattaaga attttggtgt
     3121 ttcggataat gccattttca atgccgtcgt aatcaatagc atcagaatcg tattcggcat
     3181 ttccctcaaa aatacagttt tcaatccggg catgtccgta tttaatattt agtaaatc
//
LOCUS       DAOPZK010000191         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_40383_length_3238_cov_21.524662, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000191 DAOPZK010000000
VERSION     DAOPZK010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40383_length_3238_cov_21.524662"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1915)
                     /gene="aspS"
                     /locus_tag="PKH68_08760"
     CDS             complement(161..1915)
                     /gene="aspS"
                     /locus_tag="PKH68_08760"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNX13066.1"
                     /translation="MFRTKTCGELRLADAGSKVTLAGWVQRSRKMGGMTFVDLRDRYG
                     ITQLVFNQDVNAEICEQANKLGREFVIQIEGEVAERSNKNAHLDTGEVEILVEKLHVL
                     NEAKTPPFTIEDNTDGGDDIRMKYRYLDLRRNNVRANLELRHKMTIETRSYLDKLNFL
                     EVETPVLIGSTPEGARDFVVPSRMNPGEFYALPQSPQLFKQLLMVSGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVEQEDVLNTFEGLAKHLFKSIKGIEMNDPFPRMTWH
                     DAMKYYGSDKPDLRFGMKFVELKEAVTGHGFSILDEAEYVGGICATGCASYTRKQLDE
                     LTDFVKRPQIGAKGLIYIRYNEDGTLKSSIDKFYSEEDLKKIADMFNAQKGDLILILS
                     GEKRKTRKALCELRLEVGERLGYRNKQVFAPLWVIDFPLFEWDEDVQRYFAMHHPFTS
                     PKPEDIHLLDTDTGAVRANAYDMVINGVELGGGSIRIHDSQLQSKMFELLGFTPEKAQ
                     AQFGFLMGAFQFGAPPHGGLAFGLDRFVSLFAGLDSIRDCIAFPKNNSGRDVMIDAPS
                     VIDQSQLDELNLIISLKE"
     gene            complement(1932..2354)
                     /locus_tag="PKH68_08765"
     CDS             complement(1932..2354)
                     /locus_tag="PKH68_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HNX13067.1"
                     /translation="MENLMLRQQEVLPTEEVIEKFLGESFSVYKLLIDTIIDEPYTLS
                     PEWNFYRDGNAWLCKVQHKKKTVFWLSVWEGYFKVTFYFTEKTSSGIADLDIDERIKY
                     DFINCKPNGKFLPLTLVMKNKEQLADLLKITEYKKGLK"
     gene            complement(2507..>3238)
                     /gene="fabD"
                     /locus_tag="PKH68_08770"
     CDS             complement(2507..>3238)
                     /gene="fabD"
                     /locus_tag="PKH68_08770"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNX13068.1"
                     /translation="DGTAEDLRQTRVTQPAVFLHSVISAIALGDKFNPDMVAGHSLGE
                     FSALVAAGALTFEDGLKLVYARAIAMQKACEAEPSTMAAVLGLDDDTVEEVCESIKDN
                     IVVPANYNCPGQIVISGSIEGVDQACELLKEKGAKRALKLPVGGAFHSPLMQPASEEL
                     QAAIDAAVVSQPVCPVYQNVNAYPQTDPVAIKQNLIAQLTAPVRWTQTVKNMITDGAL
                     EFVELGPGDVLKGLIKKVNPEVTVG"
BASE COUNT          892 a          741 c          641 g          964 t
ORIGIN      
        1 tttcctataa tttatattat gtcaaataca aaatcaggga atttattttc caagaattga
       61 tgtatcctct ttatatcgat ttctacaaaa tcattggaag aaaaaaaaat cgcagaattt
      121 tattcaaacg ctgcgattta attatttcta tgaataatga ttattccttc aaactaatta
      181 tcagatttaa ttcatccagc tgactttgat cgattacaga tggtgcatca atcatcacat
      241 cgcggccaga attatttttg gggaaagcga tacagtcgcg gatactgtcc aggccggcaa
      301 ataaggagac aaaacggtcg agaccaaagg ccagtccacc atgaggtggt gcaccaaatt
      361 ggaatgctcc cataagaaat ccgaactgag cctgtgcttt ttcgggtgta aatcccagta
      421 attcaaacat tttgctctgc aactgactgt cgtgaatacg aatggatcca ccaccgagtt
      481 ccaccccgtt gattaccata tcatacgcat tagcccttac agcacctgta tcggtgtcga
      541 ggagatgaat atcttcgggt tttggtgagg taaacgggtg atgcatagca aaatagcgct
      601 gtacatcctc atcccattcg aacagaggaa aatcaatcac ccaaagagga gcaaacacct
      661 gtttgttgcg atagccgagc ctctctccca cttccagacg caattcgcac agcgccttgc
      721 gggttttgcg cttctcgccc gaaagtatca aaatcaaatc acccttttga gcattaaaca
      781 tatcggcgat ttttttcaaa tcttcttccg aataaaattt atcaatggat gatttgagtg
      841 tgccgtcttc attgtatcgg atataaataa gtccctttgc gcctatctgc ggacgtttca
      901 caaagtcggt cagttcgtcg agctgcttgc gggtatagct ggcacatccg gttgcacata
      961 ttccacctac atattcggct tcatccagaa tgctgaatcc gtgaccggtt acagcttctt
     1021 tcagttctac aaatttcatc ccgaagcgta aatcaggttt atccgaacca taatatttca
     1081 tagcatcgtg ccaggtcata cgcgggaacg gatcattcat ttcaattcct tttatcgatt
     1141 tgaaaaggtg tttagcaagc ccttcgaagg tattgagcac gtcttcctgt tctacaaaag
     1201 acatttcaca gtcaatctga gtgaactcag gctgacggtc ggcacgtaaa tcttcatcgc
     1261 ggaagcattt cacaatctga aaatagcggt cgaaacccga aaccatcaac aattgtttga
     1321 atagctgagg tgattgtggc agcgcataaa attcacccgg attcatgcgt gaaggaacca
     1381 caaagtcgcg tgctccttcg ggtgtagaac ctatcagcac cggtgtttcc acctccagaa
     1441 aattaagttt atcgaggtaa ctgcgggttt ctatggtcat tttatgacga agttccaggt
     1501 tagcccttac attattcctc cgaagatcta agtacctgta tttcatgcgg atatcatcgc
     1561 ctccatccgt attatcttcg atagtaaaag gcggtgtttt agcttcattg agcacatgga
     1621 gtttttctac caggatttcc acttcaccag tgtcgaggtg cgcattttta ttgctgcgct
     1681 cggcaacttc accttcaatc tgaatgacga attcgcgtcc gagtttatta gcctgctcac
     1741 aaatttcagc attaacatcc tgattaaaaa ctaattgcgt tattccatac cggtcgcgca
     1801 aatcaacgaa agtcataccg cccattttgc ggcttcgctg tacccatccg gccagggtta
     1861 ctttactacc tgcatcggca agtctgagct caccgcatgt ttttgttctg aacatatcta
     1921 tttatgttta attattttaa tccttttttg tattctgtaa ttttcagtaa atcagcaagt
     1981 tgctccttat ttttcataac cagtgttaac ggtaagaatt ttccgttggg tttacaatta
     2041 atgaaatcat actttattct ttcatcaata tccaagtccg caattcctga agaggttttt
     2101 tcggtaaaat aaaacgttac cttgaaatat ccttcccaaa ccgaaagcca gaaaaccgtt
     2161 ttctttttat gctgaacttt gcatagccag gcatttccgt cccggtaaaa attccactcc
     2221 ggactgagcg tgtaaggctc atctatgata gtatctatca gcaacttata aaccgaaaaa
     2281 ctttctccga ggaatttttc gattacttct tcagtgggta acacttcctg ttgtctcagc
     2341 attagatttt ccatagccaa tattattaga gtgttaaatt tatatgatac aaaagtacaa
     2401 aaaaaaccga aatcccgata aggaagaaac tattttaaaa cctgacaggc acaataaatc
     2461 aattactgtg cctgtgtgtt tttgataagt aaataaaaat gaggtactac cccactgtta
     2521 cttcaggatt tacctttttg ataagccctt tcagcacatc gcccggtccg agttctacaa
     2581 attcaagagc accgtcggta atcatatttt taaccgtttg tgtccaccgt acaggggctg
     2641 tgagttgtgc aatgaggttt tgtttgatgg ctacgggatc ggtttgagga tatgcattta
     2701 cattttgata caccgggcaa acgggttggc ttaccacagc tgcgtcaatg gctgcctgca
     2761 attcctcgct ggcaggttgc atgagtggag agtgaaatgc acctcccaca ggcagtttca
     2821 atgcacgttt agctcctttc tctttcaaca attcacaagc ctgatcaact ccttcgatgg
     2881 aacctgaaat aacaatttgt ccggggcaat tgtagttggc cggaacaaca atattatcct
     2941 tgatactttc gcaaacttct tccactgtat cgtcgtcgag accaagtaca gccgccatgg
     3001 tcgatggttc ggcttcacag gctttctgca tggctattgc acgtgcataa acgagtttta
     3061 atccatcttc gaatgtgagc gctccggcgg caacaagtgc cgaaaattca cccagtgaat
     3121 ggcctgcgac catatcagga ttgaacttat cgcccagtgc aatggccgag ataactgagt
     3181 gcaggaacac tgcgggttga gtaacacggg tctgacgcag atcttcggct gtgccatc
//
LOCUS       DAOPZK010000192         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_40984_length_3205_cov_18.482857, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000192 DAOPZK010000000
VERSION     DAOPZK010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40984_length_3205_cov_18.482857"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="PKH68_08775"
     CDS             complement(<1..1769)
                     /locus_tag="PKH68_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX13069.1"
                     /translation="MKRNLITFILLVFSVVLFAQADKISVMKNSDGVKLVVNGNDFMI
                     NGMNWDYFPIGTNFNYSLWTQPDATIKAALDYEMSLLKNMGVNTIRQYTGVQPKWIQY
                     IYENYGIYTVINYSFGRYGLTIKGTWVGNTDYSNADTQELLLKDTREMVQTYKNTPGL
                     LMFLLGNENNYGLFWDGAETENIPVGDRKSTHRAKSLYKAFNDACLEIKKMDKTHPAA
                     ICNGDALFMDIIAEECKDVDVLGINSYRGISFTDIFDKIKQSTDKPIMFTEFGSDAYN
                     ALTHSEAQQDQAILDLANWKEIYANAAGMGKAGNSIGGFTFQFSDGWWKYKQTADLEV
                     HNTTASWANGGYMFDFQQGENNMNEEWFGICAKGETNVNGGYQLYPRAAYYALKSAHQ
                     YNPYSEGASLATLDRHFSTINITGAALQARGDEASLVAEQSKKYSIGIRADMSAYSTG
                     GSLITTPQKADPNADTYPDKQGFDHTQSFYFDVKAKPQDNIDIKASVNVLGNVASNPI
                     DEVFYENRVRVANLTTNGITYDFSGLERVRLYNASFSWNEKWFNLTGFYRTGHNSWGY
                     EGDLFGLYPETNYGPNLDIYHGET"
     gene            complement(1819..2712)
                     /locus_tag="PKH68_08780"
     CDS             complement(1819..2712)
                     /locus_tag="PKH68_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HNX13070.1"
                     /translation="MINYDKAICYSGFREGQAPGIKYPDYEEVKEDLLILHKRWKYLR
                     LYDCDEHAETVLRVISNEKLNFKVMLGAYITAEMNNFGCPWGGTYPEEVLISNKKKNV
                     ALIEKLIKLANQYSDIVFALSTGNEACVEWTDHYVSEKNIIEYVKMVKQGAKQPVTYC
                     ENYVPWITKLKKLASEVDFISIHTYPVWEYKEIHEAMEYTSQNYFSVAELYPDKQVVI
                     TEAGWATSSNGRGINPDNVSEENQAVYYNDLMNWSRKEKIVTFVFEAFDEPWKGSPEP
                     LEPEKHWGLFKVDRTPKLVMK"
     gene            complement(2769..>3205)
                     /locus_tag="PKH68_08785"
     CDS             complement(2769..>3205)
                     /locus_tag="PKH68_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HNX13071.1"
                     /translation="EAIPQIPVGYVDAYYEFTVRPEITKACDVILANCYPFWESCHAD
                     YSLMYMKEMFYQAVHAGEGDKNVIITETGWPSQGEDYKGALPSPENALKYFINTQLWS
                     KQDDVEIFYFTSFDESWKVGAEGDVGAFWGIWDINEKLKYQK"
BASE COUNT          788 a          720 c          630 g         1067 t
ORIGIN      
        1 gtttcaccgt gataaatatc caggttgggt ccgtaatttg tttcggggta aagtccgaac
       61 aaatctcctt cgtatcccca ggaattatgt ccggtgcggt agaaaccggt aagattaaac
      121 catttttcat tccaggaaaa acttgcgtta tacagtctta ctctctctaa acccgaaaaa
      181 tcgtatgtta tgccgttggt ggttagatta gccactctta cccggttttc gtagaatact
      241 tcatcaatgg gattggaagc tacatttccc agcacattga ccgatgcttt gatatcaata
      301 ttatcctgtg gtttggcttt cacatcgaaa taaaatgact gggtgtgatc aaagccctgt
      361 ttgtcaggat acgtatcagc gtttggatcc gctttttgag gagttgtaat caaacttcca
      421 cctgtgctgt aggcactcat atcggctctg ataccgatgc tgtatttctt tgattgttcg
      481 gctacaagcg atgcttcatc tcctcttgcc tgtaaagctg ctccggtaat attgatagtt
      541 gaaaaatgcc ggtcgagtgt ggctaatgag gctccttcgg aataaggatt gtactgatga
      601 gcacttttga gggcatagta tgcggcacgc ggataaagtt gataaccacc attcacgttt
      661 gtttcaccct tggcacaaat accaaaccat tcttcgttca tgttgttttc accttgctgg
      721 aaatcaaaca tataaccgcc atttgcccac gaagcagtag tgttgtgcac ctccagatcg
      781 gcggtttgtt tgtatttcca ccatccgtca ctgaactgga aggtaaatcc tccgatgctg
      841 tttccggctt ttcccattcc ggcagcgttg gcgtaaattt ctttccagtt tgccaagtct
      901 aatattgcct gatcctgttg tgcttcggag tgagttagcg cattataagc atccgatccg
      961 aattctgtaa acataatcgg tttgtccgta ctttgtttta ttttatcaaa aatatcggta
     1021 aaagatattc cgcgataact gtttataccc agcacatcca catccttaca ttcttcggca
     1081 ataatatcca taaaaagtgc atcgccgtta cagatagctg cggggtgggt tttgtccatt
     1141 tttttaattt ccagacaggc atcgttaaag gctttgtaca aggatttggc tcgatgggtt
     1201 gattttctgt cgcctaccgg aatgttttct gtttcggctc catcccaaaa cagtccgtag
     1261 ttattttcat tgcccaacag aaacatcaaa agacccggtg tgtttttgta cgtttgcacc
     1321 atttctctgg tatctttgag cagcagttct tgcgtatcgg cattcgaata atcggtattg
     1381 cctacccagg ttcctttgat ggtaagtccg taccggccga atgaataatt gattacggtg
     1441 taaataccat agttttcata gatgtactgt atccatttag gctgcactcc tgtgtactga
     1501 cgaatggtgt ttacacccat atttttcagc aatgacattt cgtaatccag cgcagctttg
     1561 atggtagcat ccggctgtgt ccacaggctg taattgaaat tggtgccaat tggaaaataa
     1621 tcccaattca ttccgttaat cataaagtcg ttgccattaa caactaattt caccccgtcg
     1681 ctgtttttca ttacggatat tttatctgcc tgcgcaaaca aaacgactga aaacaccaac
     1741 agaataaaag ttatcagatt tcttttcatt gtgttttttg tttttgtgtt agtattaatt
     1801 tgttaatcta atttgtattt atttcattac cagtttgggt gttctgtcca ctttaaataa
     1861 accccagtgc ttctcgggct ccaaaggttc gggcgaacct ttccagggtt catcaaaagc
     1921 ttcaaaaaca aatgtcacta ttttctcttt cctgctccaa ttcatcaggt cattgtaata
     1981 aaccgcctgg ttttcttcgc tcacgttgtc gggattaatt ccccgtccgt ttgaactggt
     2041 tgcccagccg gcttcggtta ttaccacctg cttatcggga taaagttctg ccaccgaaaa
     2101 atagttttga ctggtatatt ccatggcttc gtgaatttct ttatattccc acacggggta
     2161 ggtgtggatg gatataaaat ccacttcgct ggccagtttt ttcagttttg taatccaggg
     2221 aacgtaattt tcgcaataag ttacgggttg cttagcgcct tgttttacca ttttcacgta
     2281 ttcgatgatg tttttttctg acacataatg atctgtccat tccacacagg cctcatttcc
     2341 ggtcgataat gcaaaaacaa tatcgctgta ctgatttgcc agttttatta gtttttcgat
     2401 gagtgcaacg tttttcttct tatttgaaat cagcacttct tcgggatacg ttccgcccca
     2461 cggacaaccg aaattgttca tctcggccgt gatgtaagct cccagcataa ctttaaagtt
     2521 cagtttttca ttgctgatca ctctcaacac tgtttcggcg tgctcgtcgc agtcgtaaag
     2581 tcgtaaatat ttccagcgtt tgtgaagtat cagcaaatct tcctttactt cttcataatc
     2641 gggatatttt atacccggtg cctgcccttc cctgaagcct gaataacaga tggctttatc
     2701 gtaatttatc ataaaaatat tttgcccgct taatccctta tttgagagtt taccggtgac
     2761 tgtctgaatt atttctggta ttttagtttt tcattgatgt cccatatacc ccagaatgca
     2821 cccacatcac cttcggcgcc cactttccag ctttcgtcga aagaggtgaa gtaaaaaatc
     2881 tcaacatcat cctgctttga ccacaactga gtattgataa aatatttcag cgcattttca
     2941 ggtgaaggaa gcgccccttt gtaatcctcg ccctggcttg gccatccggt ttcggtaata
     3001 attacatttt tatcgccttc gccggcgtgt acagcctgat aaaacatttc cttcatatac
     3061 atcaatgaat aatcggcgtg gcagctttcc cagaatggat agcagttggc cagaattaca
     3121 tcacaggctt ttgtgatttc aggacggacg gtgaattcat aataggcatc cacatacccc
     3181 accggtattt gaggaatggc ttctt
//
LOCUS       DAOPZK010000193         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_43100_length_3098_cov_24.714427, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000193 DAOPZK010000000
VERSION     DAOPZK010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43100_length_3098_cov_24.714427"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..510
                     /locus_tag="PKH68_08790"
     CDS             97..510
                     /locus_tag="PKH68_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13072.1"
                     /translation="MRKLIIVAVLAIFICSIAPAQIKVVLVQDYEQMKANGTKQLNPT
                     PKKSTVRKAAFNATETTDDEETIASDNTNNETNLSETAASTSKQAAVKGNALKANTAN
                     STSATAAVEKKSTASSGSDYVKRLRERQKAARTGR"
     gene            709..2610
                     /locus_tag="PKH68_08795"
     CDS             709..2610
                     /locus_tag="PKH68_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNX13073.1"
                     /translation="MQQYLDILKTYWGYDEFRPLQADIIQSIASGRDTLGLMPTGGGK
                     SLTFQVPALAMEGVCIVVTPLIALMKDQVENLKQRGISAVAIYSGMTHKEIMLTLDNA
                     VYEAYKFLYVSPERLATDIFIVKVKQMKVCMIAVDESHCISQWGYDFRPSYLKIAAIR
                     DFLPDAPVLALTATATPEVVDDIQEQLHFREKNVFRKSFHRSNLVYVVRTVENKDEYL
                     LKILTKVPGTSVVYVRNRKKTKEIADFLNTNGISAEHFHAGLKNEIKDARQARWKSGE
                     TRVIVATNAFGMGIDKPDVRTVIHMDLPDSLEAYFQEAGRAGRDEKKAYATLLFNKTD
                     ITKLRKRVADTFPPKETVAKVYDAIGNYYELAVGSGLDATYVFDIGDFCGKFHFPVLV
                     AYNSVKILQQAGYLELTDEQDSSSMVVFTVKKDQLYAYKSTPEQDKLIHILLRSYTGL
                     FTDMAAINEETLATRLGWSHNQVYEQLIALSREHIIKFIPRKKTPFLTYTARREQLSR
                     LFITPEAYDDRRKRYIQRVKSVLDYAKEENYCRSQILLNYFGENESQPCGKCDICLRN
                     KENQLTEPEFQQIQSDIIRLLRDAATPLGTLLMKLPYKEQKILQVIRFLLDQHQISQN
                     EQMKLQTNI"
     gene            2704..>3098
                     /locus_tag="PKH68_08800"
     CDS             2704..>3098
                     /locus_tag="PKH68_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13074.1"
                     /translation="MYKQNKLKYFRWHWGDRYDGWRVRNVDAVFSVIPYFLRTRMDAQ
                     NYFEQRIPIDHIETFIREHKEDIPGLSIMHVVIASMIRLVSQRPYLNRFIMWNKIFAR
                     NYINFSIVIKRSLSDEGEETLIKPYFQPT"
BASE COUNT          967 a          696 c          646 g          789 t
ORIGIN      
        1 taaaatgatt tttcagaaaa aaatagtcga tatttatcgt cggcttaata ttttatttat
       61 ctttgaacaa taaattaaaa gcacacatat atacccatga gaaaactaat tattgttgct
      121 gtcttagcta ttttcatctg cagtattgca cccgctcaga ttaaagtggt gctggttcag
      181 gattatgaac agatgaaagc aaacgggaca aaacaactaa acccaacccc aaaaaaatca
      241 actgtacgca aggctgcttt caatgcaaca gaaacgaccg atgatgagga aacaattgct
      301 tcggataaca cgaataacga aacaaatctt agcgaaacgg ctgcttccac atccaaacag
      361 gcagctgtca aaggcaatgc actgaaagcc aatacggcaa atagcacttc tgccacagcc
      421 gctgtggaga aaaaaagtac agcttcttcc ggctcggatt atgtaaaacg actgcgtgaa
      481 aggcagaaag cagcccgaac aggcaggtaa aacagtcccc gggagttgca tatatcttac
      541 ttcttaaaag ttttcatccc tgacagaata aacatctctc acaatatcag ctaattatta
      601 gcactactga tttgtgaaga ataaaattga aaatcattca gctaccgcgg ccgggtgatt
      661 tttctttttt ttagttacat ttgcattcca cttaaaaatc cttttcgttt gcaacaatac
      721 ctggacatac tgaaaactta ctgggggtac gatgagttcc gccctcttca ggctgacatc
      781 attcaaagca tagcttcagg acgcgatacg ctggggctga tgcctaccgg cggtggcaaa
      841 tcactcacct ttcaggtacc ggcactggcc atggaaggtg tttgtattgt agtgacgccg
      901 ctcatagcac tgatgaaaga tcaggtggaa aatctcaaac aaaggggaat atcggctgtt
      961 gctatctatt cgggcatgac ccacaaggaa atcatgctta cactcgataa tgcggtgtat
     1021 gaagcctata aatttttgta cgtttcaccc gaacggctgg ccaccgatat ttttattgtc
     1081 aaagtgaaac agatgaaagt ctgtatgata gccgtggatg agtcacattg catttcacag
     1141 tggggctacg attttcgtcc gtcttacctt aagattgctg ccattcgcga ttttctgccc
     1201 gatgccccgg tgctggcact caccgctact gccacacccg aggtggtgga cgatattcag
     1261 gagcaacttc atttccgcga aaaaaatgta ttccgaaaaa gctttcaccg ctccaacctg
     1321 gtgtatgtgg tgcgtaccgt ggaaaataag gatgaatacc tgctgaagat tctcacgaaa
     1381 gtacccggaa cttcggtggt gtacgtgcgc aaccggaaaa aaacaaagga aattgctgat
     1441 ttcctgaata caaacggaat ttcagccgaa cattttcatg ccggactgaa aaatgaaatc
     1501 aaagacgccc ggcaggctcg ctggaaatcg ggcgaaacgc gtgtgattgt cgctaccaat
     1561 gcctttggga tgggtatcga caaacccgat gttcgtacag tgattcacat ggatttaccc
     1621 gattcgctcg aagcttattt tcaggaagcc ggacgcgccg gacgcgatga gaagaaagcg
     1681 tacgccactc tgcttttcaa caaaacggat ataacaaaac ttcgcaaaag ggtggctgat
     1741 acatttccac ccaaagaaac cgtagcaaag gtgtacgatg ccatcggaaa ttactacgaa
     1801 ctggcagtag gttcgggact ggatgccacc tatgtgtttg atattggaga tttttgcggt
     1861 aagtttcatt tcccggtgct ggtggcttac aattcggtta aaatactgca acaagccggt
     1921 tatctggaac ttaccgacga acaggacagt tcctccatgg ttgtttttac cgtcaaaaag
     1981 gatcaactct acgcatataa atccacaccc gaacaggaca aactcattca catcctgctg
     2041 cgctcctaca ccggtctgtt taccgatatg gcagctatca acgaagagac gctggctaca
     2101 cgactgggtt ggtcacacaa tcaggtgtac gaacagctca tagcacttag ccgcgaacat
     2161 atcattaaat ttattccccg aaagaaaaca cctttcctca cttatacggc acgcagagag
     2221 cagttgtcaa ggctttttat tacccccgaa gcgtatgacg accgccgtaa acgctatatt
     2281 cagcgggtaa aaagtgtatt ggattatgcc aaagaagaaa attactgcag aagtcaaatt
     2341 ctgctaaatt attttggcga aaatgaaagt caaccctgcg gaaaatgtga tatatgcctc
     2401 cggaataaag aaaaccaact cactgagccg gagtttcagc aaattcaatc agatatcatc
     2461 cgactgctcc gggatgcggc aacaccactc ggaacgctgc tgatgaaact gccgtataag
     2521 gaacaaaaaa ttttgcaagt gatccgcttt ttgcttgacc agcatcagat tagccaaaac
     2581 gaacagatga aactgcaaac caacatatag cagcttagct aaaaaagtac aggaaacgcc
     2641 cttttttcca aaaagaaaaa ttatctttga aaccgcaaat aaaattaatc aaatttaata
     2701 caaatgtata aacaaaacaa actcaaatat tttcgctggc attggggcga cagatatgac
     2761 ggttggcggg tacgcaacgt ggatgccgta ttttcggtaa ttccctattt tctgagaaca
     2821 cgcatggatg ctcagaatta ttttgaacaa cgtattccga ttgaccacat cgaaacattt
     2881 atccgcgaac ataaggaaga tatacccggt ctgtcaatca tgcacgtggt catcgcctct
     2941 atgattcgac tggtctctca acgaccatac ctgaatcgct tcatcatgtg gaataaaatt
     3001 tttgcccgta attatatcaa tttttctatt gtaataaaac gttctttgtc tgatgagggc
     3061 gaagaaacat tgataaaacc ttatttccag cctaccga
//
LOCUS       DAOPZK010000194         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_43288_length_3089_cov_22.449901, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000194 DAOPZK010000000
VERSION     DAOPZK010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43288_length_3089_cov_22.449901"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..1148
                     /locus_tag="PKH68_08805"
     CDS             387..1148
                     /locus_tag="PKH68_08805"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNX13075.1"
                     /translation="MNLKKTILLAALLTLFLPGRAQIRPNETLIFNGSYELSGMMTSL
                     AQITMKTEPVKTSSKSYLRLSAQVATYTKWDSFFKIRDLYESYVHPYSLKPTLFKRNV
                     YEGGYTKVEKYTFNAAGTQVATVTQRRNRPQETGNVTISANTLDAVTLVYKLRTVDLS
                     KVAKGQSLRFSMIFDRKEHFVWVKMMGKETVSAGNLGKIECYKLSISAKTDKLRGVDK
                     NLVWLSADAKRIPCMMKFSIPVGVGQVTLSKATGI"
     gene            1159..1473
                     /locus_tag="PKH68_08810"
     CDS             1159..1473
                     /locus_tag="PKH68_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13076.1"
                     /translation="MRKLFIILAAVFAVVGLVFAILPMGSIAFLPIILAIVLALVAMW
                     KSNDNQKKIPKWILVIALAILVLVTVKVVFVKDKVVVDQQFIQENVDSKEEAQQELEG
                     LE"
     gene            1916..3088
                     /locus_tag="PKH68_08815"
     CDS             1916..3088
                     /locus_tag="PKH68_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13077.1"
                     /translation="MKYFTTILLLFSFMMGYSQNTAISEAENLIAIKNYKSAYKVLQS
                     ADTANQIPEIVVAKTDLLLNHNIGANSLRVFALTDSAMNAAGTRNPDLYVRFAADSIL
                     NALIEKNPTNYNLYKTLGDYYLAVNLAGEEDWFLSDSAVIAGIRSNYQMAYEHGVSDY
                     KSLNGLGYTYMLDEDFETALPYLEQSVKLNKNFPVSQSNLADVYFSTGQLDNALLAAQ
                     QAFGLYTAPQEKAEAARKIAEIYIEKKQGDKAVMYYLKADDLVPNDYLTLMPLLKVEM
                     ELNNPDYANRTNQVITLAPDNPAVYQDVYKIYSENEKEKEFIDFLDSQKVYYRSNVRA
                     LAHNHFYTAVAQYEQDKWVDAKINFEKARSLFRNIFKSNHSVFKVIDSYTNAIRKK"
BASE COUNT          952 a          681 c          635 g          821 t
ORIGIN      
        1 ttactgtttc gggaactttt tcccgtcccg aacttgtttc gggaactgat tactgattac
       61 tgatcccgaa gtttcgggac tgattactaa ttactgttca ctgattactg ttcactgatt
      121 actcattact gtttactgat aaaaaacgac ctttagactt ttttagttag ttggtttatc
      181 ctgcattatc ctaaaattat taattttgca cctggaaaaa acaataatca gttcctatcc
      241 ggattattca cggggtgacc tggagtcgtc cggtgaataa tggcgttgat acaattttat
      301 ttttatcctg ccgttaataa taacggtttt tgatgaaaga ccacctgatt ttgcacagca
      361 tttatcaaaa cacaaaaaca ttcaatatga atttgaaaaa aacaatttta ctggcagccc
      421 tgcttacact cttcctaccc ggccgggcac aaatccgacc caacgagacc ctgatattca
      481 atggttcata cgaattgagc ggcatgatga ccagcctggc gcaaatcacc atgaaaacag
      541 aacccgttaa aaccagttcc aaatcgtacc tgaggttatc ggcacaggtg gcgacctaca
      601 ccaaatggga ttcctttttc aaaatacgcg acctctacga gtcgtacgta cacccctatt
      661 cgctcaaacc cactctcttc aagcgcaatg tgtacgaagg tggttatacc aaagttgaaa
      721 aatacacctt caacgcagcc ggcactcagg tggctaccgt tacacagcgc cgaaacagac
      781 cacaggaaac cggaaatgtg actatcagtg ccaataccct ggatgctgta acacttgtgt
      841 acaaactgcg cacggtggat ctctcgaaag tggctaaagg tcagtcgctg cggttctcga
      901 tgattttcga cagaaaagaa catttcgttt gggtgaaaat gatgggaaaa gaaacggtat
      961 cggccggaaa tctgggcaaa atagaatgct ataaactatc catatcggct aaaaccgaca
     1021 aactgagagg cgtggacaaa aacctggtgt ggctctccgc cgatgccaaa cgcatcccgt
     1081 gcatgatgaa attcagcata ccggtaggtg taggtcaggt taccctctcc aaagcaacag
     1141 gaatttaatt caaagaatat gagaaaacta tttattattt tagccgcggt atttgcggtt
     1201 gtagggttgg tttttgccat tttaccaatg ggcagcatcg ctttccttcc cattatttta
     1261 gctatcgtgc tggcactggt cgctatgtgg aaatcgaacg ataatcagaa aaaaatacct
     1321 aaatggatac tggtaattgc tttggctata ctggtactgg taacggttaa agtggttttt
     1381 gtaaaggata aagtggtagt ggaccaacag tttattcagg aaaacgtaga ttcgaaagaa
     1441 gaagcccagc aggaacttga agggctggaa taatccggtt tttcagcata aataatgaga
     1501 attatcggta gttaccgaaa attctctcat taacatttcc ggatataacc agctgcaatg
     1561 cagttgatta tatccggatt cttatgtccg gcagcttcct gatcaggata gaataaagca
     1621 gggtgccaaa accggcaagg ttcttttcaa ataaatttct atctttgttt tatgttaaaa
     1681 actttctttc tgaagctcaa ttcagtcaca ggactttttg acacaagatt ttctgacaca
     1741 attaaaattt gcaaacggat tgttctaaag caaaataaat gaaacaggtt tgccgttata
     1801 tgttaatgtg cagtgacaag gcacactatt tgtttgaacc tgaataactg atgattttga
     1861 agaatggcct gaccggtaat tttcacttta accacaaaaa aagacactta ttgaaatgaa
     1921 atattttaca actattctcc ttctcttttc ctttatgatg gggtattctc aaaataccgc
     1981 catcagtgag gctgagaatc tgatagctat aaaaaactat aaatcggcct ataaagtact
     2041 ccaaagcgcc gatactgcca atcaaattcc tgaaatcgtc gttgctaaaa cagatctgct
     2101 gctcaatcac aacattggag ccaacagcct gcgggtattt gcactcactg atagtgccat
     2161 gaacgctgcc ggaacgcgta atccggattt gtacgtacgc tttgccgccg atagcattct
     2221 gaatgcattg attgaaaaaa atccaaccaa ttacaatctt tacaaaacac tgggcgatta
     2281 ctacttagcc gtaaatctgg ccggtgagga ggactggttc ctgtccgact cagctgtaat
     2341 agccggtatc cgcagcaatt atcagatggc ctatgaacac ggagtatccg attacaaatc
     2401 gctgaacggg ctgggctaca cttacatgct cgacgaagac tttgaaacag cgctccccta
     2461 cctggagcag tcggtgaaac tcaataaaaa ctttccggtc tcccaatcca atctggccga
     2521 tgtgtatttt tccaccggtc aactcgacaa tgcactgctg gccgcacagc aggctttcgg
     2581 gctttacaca gctcctcagg agaaagcgga ggcagcccgc aaaattgcag aaatttacat
     2641 cgaaaagaaa caaggtgata aggcggtgat gtattacctc aaagccgatg acctcgtacc
     2701 caacgattac ctcaccctca tgcccctgct caaagtggaa atggaactga ataatccgga
     2761 ttacgccaat cgtaccaatc aggtgataac actggcaccc gacaatccgg cggtgtatca
     2821 ggatgtatat aaaatctaca gcgaaaatga aaaggaaaaa gaatttatcg atttcctgga
     2881 tagccagaaa gtgtattatc gctccaatgt gcgggcactg gcacacaacc atttttacac
     2941 ggccgtagct cagtacgaac aggacaaatg ggtggatgcc aaaatcaatt tcgaaaaagc
     3001 cagaagcctg ttccggaata tcttcaaatc gaaccacagc gtgttcaaag tgattgactc
     3061 ttataccaat gcgataagga aaaaataaa
//
LOCUS       DAOPZK010000195         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_43721_length_3067_cov_24.600266, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000195 DAOPZK010000000
VERSION     DAOPZK010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43721_length_3067_cov_24.600266"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="PKH68_08820"
     CDS             complement(<1..1450)
                     /locus_tag="PKH68_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX13078.1"
                     /translation="MNEEQIKNSKGEYGASSIQVLEGLEAVRKRPAMYIGDTSLKGLH
                     HLVYEVVDNSIDEAMAGYCDIIHVTINEDNSIKVTDNGRGIPVDMHEKEGKSALEVVM
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSTKLRVEVARDGVLYAQDYSCGIPLAP
                     VAEVGETDRQGTSVTFLPDNTIFSESVYKYDILANRLRELAYLNAGITLTLTDKRELQ
                     EDGSYKKEVFFSKEGLKEFVEYIDESRENLIEQVIHISTEKNGIPVEIALLYNTSYNE
                     NIHSYVNNINTIEGGTHLTGFRRGLTRTLKKYAEDNKLLEKLEKAKIEISGDDFREGL
                     TAIISIKVAEPQFEGQTKTKLGNNEVMGSVEMAVSEALGNYLEENPKDARTIVDKVVL
                     AATARHAARKARELVQRKSPLSGGGLPGKLADCSSKDPQKCELFLVEGDSAGGTAKQG
                     RSREYQAILPLRGKILNVEKAMQHKVLDSEEIR"
     gene            complement(1660..2970)
                     /locus_tag="PKH68_08825"
     CDS             complement(1660..2970)
                     /locus_tag="PKH68_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNX13079.1"
                     /translation="MKTENLIYRILFFLLGMFIMSFGVALSIRSNLGTTPISAPPYVL
                     SLFTSLSVGTYTIFVNVLFVFLQILILRKDFQLLQLAQIPAVILFGWFIDLSLAGISG
                     LTPPNYLAEWLVSLLSSAVLAFGVFLQVKSDITLLPGEGLVMALTKAYKLEFGKTKII
                     LDSLLVIISVIVSFVFLKNLQGVREGTVAAALLVGGIIRFYSANIHTFDKLLGLEQNV
                     NVSPQKKIDTQVITISREYGSGGHEIGELVAKKLGFAFYDTNLIDLSALEGGFTPEFV
                     KENEQKLKNNLLYSLYKNNYAYVNEVMPPQDALFLVQSKVIRDISERSSCVIVGRSAD
                     FILKSHPHCFNVFVHADKAFRLERIINNYGFDPADAEAQMMQKDRERINFSKYYAHRA
                     WGHPDNYHMTVDSSTFGIDIAAALLVDAWRKAVALAPETESNLN"
BASE COUNT          838 a          703 c          643 g          883 t
ORIGIN      
        1 tgcggatttc ttcgctgtcg agcactttgt gctgcatggc tttttccacg ttcaggattt
       61 tccctctcaa aggtagaatt gcctgatatt cgcgactgcg accctgttta gctgtaccgc
      121 cggcagagtc cccttcgacc agaaacaatt cacatttctg tggatctttg cttgagcaat
      181 cggccagctt gcccggcaat cctccacccg acaggggcga ttttcgctgt accagttcgc
      241 gtgctttacg ggcggcatga cgagctgtgg cagccagtac aaccttatcg acaatggtac
      301 gggcatcctt cggattttct tccagatagt ttccaagcgc ttcgctcaca gccatttcca
      361 ctgagcccat cacttcattg ttaccaagtt tagttttcgt ttgaccttcg aactgaggtt
      421 cagccacttt aatggagatg atagctgtca atccttcacg gaagtcatca ccgctgattt
      481 ctattttggc tttttccagt ttttcgagca gtttattgtc ctctgcatat tttttcagcg
      541 tacgggtaag tccgcgacga aaaccggtaa ggtgagtacc tccttctatc gtattgatat
      601 tattcacata ggaatggatg ttttcgttgt atgaggtgtt gtaaagcagc gcaatttcca
      661 ccggtattcc atttttttca gtagaaatat gtatcacctg ttcaatgaga ttttcgcggc
      721 tttcatcgat gtactccaca aattctttca atccttcttt ggagaaaaaa acttcctttt
      781 tataacttcc atcttcctgt agttcacgtt tgtcggtgag ggtaagtgta attcccgcat
      841 tgagataagc aagctcccgc aggcggttgg ccagaatatc gtatttataa accgattccg
      901 aaaaaatggt attatcgggc aggaaagtca cggaagttcc ctgccggtca gtttcaccta
      961 cttcggctac aggagccagc gggataccgc aggaataatc ctgagcatac agcaccccat
     1021 cgcgggcaac ctccacacgg agtttggtgg agagtgcatt cacacatgac acacccacac
     1081 cgtgcaatcc gcctgaaacc ttataactac ccttatcaaa tttacctccg gcatgaagta
     1141 cggtcattac cacttcgagg gcagattttc cttctttttc gtgcatatcc accgggatac
     1201 cacgtccgtt gtcggtaacc tttattgaat tatcttcgtt gattgttacg tgtattatat
     1261 cgcaataacc ggccattgcc tcatcaatgg aattgtcaac cacttcgtac accaggtggt
     1321 gcaagccttt cagactggta tcgccaatgt acatggccgg acgcttacgt accgcctcta
     1381 agccttcaag aacctgaata ctactcgcac catactctcc tttggaattt ttaatttgct
     1441 cttcgttcat tttgttatat cttatttatt atctgcttgt tacatcatta tttgaaatta
     1501 aaaataaatc tcattttgta tactaaaaaa catacaaata tacgataaaa aattggatta
     1561 atgaagacac ggccgaagtg atttttaata aaattccact tcaatacagc ttacattgta
     1621 aattgatttc aatcagtaaa aatgataaca gcggggaaat taattgaggt tactttcggt
     1681 ttcaggtgcc agtgccacgg ctttgcgcca ggcatcaacc agaagggcag cggctatgtc
     1741 aatgccaaaa gtggagctgt ccactgtcat gtggtaatta tcgggatgcc cccaggcacg
     1801 gtgtgcataa tatttactga aattaatccg ttcgcggtct ttttgcatca tctgagcttc
     1861 ggcgtcagcg gggtcaaatc cgtaattatt gatgatgcgc tcaagacgaa atgctttatc
     1921 ggcatgtaca aacacattga agcaatgcgg atggcttttg agaatgaaat cggccgaacg
     1981 tccaacaatt acacaggacg atctttcgct gatatcccgg ataactttac tttgtaccag
     2041 aaaaagtgcg tcttgcgggg gcatcacctc attgacgtaa gcgtaattat ttttatagag
     2101 ggaataaagg aggttatttt tcagcttttg ttcgttttcc ttcacaaatt ccggtgtgaa
     2161 accaccttcg agggccgaaa gatcgatcag attggtgtcg tagaaggcaa aaccgagttt
     2221 tttggccacc agttcaccga tttcatgacc tccactgcca tattcgcggg agatggtaat
     2281 gacctgagta tctattttct tctgaggtga tacgtttaca ttttgttcca aaccaagcag
     2341 tttatcaaac gtatggatat tggcggaata gaatcgaata attccgccca cgagcaacgc
     2401 agcggccaca gtgccttcac ggactccctg cagatttttc aggaaaacaa atgaaacgat
     2461 taccgaaata atcaccaaca gactgtcgag tatgattttc gttttaccga attcgagttt
     2521 atacgctttg gtaagagcca tgaccaggcc ttcgccgggc agcagtgtta tatcagattt
     2581 cacctgcaga aaaactccga atgccagcac tgcactacta agcaaactca cgagccattc
     2641 tgccaggtaa tttgggggtg ttaatcccga aatgcccgcc agcgaaaggt cgataaacca
     2701 cccaaaaagt atcactgcag gtatctgagc caattgcagg agctgaaaat cttttcgaag
     2761 aattagtatt tgcagaaaaa caaaaaggac attaacaaaa atggtataag tacccacact
     2821 gagtgatgta aagagactca acacataagg cggtgcagat atgggagttg tacccagatt
     2881 tgaccggata gaaagagcaa ccccaaagga cataataaac atcccaagga gaaagaatag
     2941 tatcctgtaa atcaggttct ctgttttcat tttcgctttc tgttagttga taaccggcac
     3001 gacactcgct gtaatcgggg atacaaaatt aagaattttg gatcatataa atcgtagcaa
     3061 aagcact
//
LOCUS       DAOPZK010000196         3060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_43857_length_3060_cov_23.177038, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000196 DAOPZK010000000
VERSION     DAOPZK010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43857_length_3060_cov_23.177038"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /gene="alaS"
                     /locus_tag="PKH68_08830"
     CDS             complement(<1..1884)
                     /gene="alaS"
                     /locus_tag="PKH68_08830"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNX13080.1"
                     /translation="MTSKEIRQSFLDFFESHGHKIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKNIILGNDPIKYPRVADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAITWAWEYLTEVLKLDKNRLYVTVFEGSEYEKLERDNEAAEIWSQFLPADRIINGN
                     KKDNFWEMGDTGPCGPCSEIHIDIRPDSERAKVDGLSLVNQSHPQVIEIWNNVFMQFN
                     RKADGSLEELPAKVIDTGMGFERLCMAIQGKTSNYDTDVFQPVIEEIGKLSQIKYGNS
                     EKTDIAMRVIADHIRTIAFSIADGQLPSNAKAGYVIRRILRRAVRYGYTFLGHRQAFM
                     YRLMPVLCEVMGGAYPELTAQKSLIEKVMKEEEEAFLRTLETGIKLLEKVMDDASKSN
                     KKEISGVDAFTLYDTFGFPLDLTELILREHNFTVNINEFNAEMQKQKDRARNAAVVET
                     GDWVTVREGDSVFVGYDKTETETEILRYRKVQQKAISFYQIVLSETPFYAEMGGQVGD
                     KGWLTANDEKIEIFDTKNENNLTVHLANQLPADVNVRLKAAINVEKRKDTERNHSATH
                     LLHEALREVLGKHVEQKGSYVSPESLRFDFSHFQKLTTEELRAVEKIANRKIRENYPL
                     QESR"
     gene            2108..>3060
                     /locus_tag="PKH68_08835"
     CDS             2108..>3060
                     /locus_tag="PKH68_08835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX13081.1"
                     /translation="MAKKKFYFNSETLDYEEIQINLAYRLKQVLFHAMSGIFLGLLFF
                     FLFVSIIKSPREKEISLQKSRMEGQYKALQGQMSDIQGILTDLQQRDDNLYRVVFQAE
                     PIPYEVRRTANTNADYYEQLLRMTNSQIVVETTKKLNELKKQLYVQSKSYDEIVVLAQ
                     NKEKMLEAIPAIQPVANKDLTRVASGYGMRMDPIYHSRRFHAGMDFTAPTGTDIYATG
                     NGRVLSAGWQQGYGNCVEIDHGFGYVTLYGHMHSISVNAGQTVNRGEVIGLVGNTGKS
                     TGPHLHYEVHYKGEIMNPQNYYFLDLTPEEYDKMVQLSNNAG"
BASE COUNT          786 a          682 c          669 g          923 t
ORIGIN      
        1 acggctttcc tgcagcgggt agttctcgcg gattttacgg ttggctattt tttcaacggc
       61 gcgcaattct tcggtagtca gtttttgaaa atgtgaaaag tcaaaacgca gactttcggg
      121 acttacatag gaacccttct gctccacgtg tttgccaagc acttcacgta aagcttcgtg
      181 cagcaggtgt gtggcggaat gattccgttc ggtatccttg cgtttttcca cattaatggc
      241 tgctttcaat cttacattca catcagcagg cagttgattg gctaaatgta ccgtcaggtt
      301 gttttcgttt tttgtatcaa aaatttcaat tttctcatca ttggctgtaa gccacccttt
      361 atcgccaacc tgaccgccca tttcggcata aaacggggtt tctgacaaca caatctggta
      421 aaaagagatg gctttttgtt gtacctttcg ataacggaga atttcagttt cggtttccgt
      481 tttgtcataa cctacaaaca cactgtcgcc ttcgcgtacg gtaacccagt cgccggtttc
      541 cacgactgct gcgttgcggg ccctgtcttt ttgtttttgc atctctgcat tgaattcatt
      601 gatattgacc gtgaagttgt gttcgcgtag aataagttca gtcagatcca gcgggaaccc
      661 gaaagtgtca taaagcgtaa aggcatctac acctgaaatt tctttcttat ttgattttga
      721 agcatcgtcc atcacctttt ccaatagttt tatccctgtt tccagtgtac gcaggaatgc
      781 ctcctcttct tctttcatca ctttttcaat cagacttttt tgagctgtga gttcggggta
      841 tgcacctccc atcacttcgc acaaaaccgg cattaaccgg tacataaagg cctgacgatg
      901 tccaaggaat gtgtatccat agcgtacagc acgtcgtaaa atccgtcgga ttacatatcc
      961 ggctttagca ttggagggca gctgaccatc ggcaatggag aaggcgatgg tgcgaatatg
     1021 gtcggctatt acgcgcatgg caatgtctgt tttctccgaa ttgccgtatt taatctggct
     1081 gagtttgcct atttcttcaa tgacgggctg aaaaacatcg gtgtcatagt tcgaagtttt
     1141 gccctgaatg gccatacaaa ggcgttcgaa acccattccc gtatcaatta ccttggcggg
     1201 aagttcttcg agcgaaccat cggctttgcg gttgaactgc atgaatacgt tgttccagat
     1261 ttcaatgacc tgaggatggc tttggttcac cagactaagt ccgtcgactt tggcacgctc
     1321 actgtcgggg cggatatcga tatgtatttc ggaacacgga ccgcagggac ctgtgtcacc
     1381 catttcccaa aaattatctt tcttattacc gttaataatg cggtctgccg gaagaaactg
     1441 cgaccagatt tcggcagcct cgttgtcacg ttccagtttt tcgtattccg aaccttcaaa
     1501 aacggttaca tacaggcggt ttttgtccag tttcagcact tcggtgaggt attcccacgc
     1561 ccaggtaatg gcttccttct tgaaataatc gccgaatgac cagttgccga gcatttcgaa
     1621 catggtgtgg tgataggtgt catgtcccac ttcttccagg tcgttgtgct tgccgcttac
     1681 gcgaaggcat ttctgcgagt cggccacgcg gggatatttt attgggtcat taccgagaat
     1741 aatatttttg aattggttca ttcccgcatt ggtgaacatc agcgttgggt catcttttat
     1801 aaccatgggt gccgaaggta caattttatg cccgtgtgat tcgaaaaaat cgaggaatga
     1861 ctgtctgatc tcttttgatg tcatagtgaa tttaaatcta atgatttttt agttttagta
     1921 aattgctatt tgcttcttat tcatccaaat agccaacccg ttaatattta tcaatataag
     1981 gtgcaaaagt acttcaaaat tattactttt gcatcaatat tactgtaaaa acaggtgtat
     2041 ttgattgtaa aaatacattc aacaaacatc tttcttcctg tttttactgt tttttatccc
     2101 tttttttatg gctaaaaaga aattttattt caattccgaa acacttgatt acgaggaaat
     2161 tcagattaac ctggcctaca ggctcaagca ggtgctgttt catgctatgt cgggtatatt
     2221 tttgggatta ttatttttct ttttgttcgt ttccattatt aaatcgcccc gcgaaaaaga
     2281 aatttccctg cagaaaagcc gtatggaagg tcagtataaa gccctccagg gtcaaatgtc
     2341 cgacattcag ggaatactta ccgatttaca gcaacgtgat gataaccttt atcgggtggt
     2401 atttcaggca gaacccattc cttatgaagt ccgccgaact gcaaatacga atgccgatta
     2461 ctacgaacag ttgctccgca tgaccaattc gcagatagtg gtggaaacca ccaaaaaact
     2521 gaacgaattg aaaaaacaac tctacgttca gtcaaaatca tacgatgaga tagtggtact
     2581 cgctcaaaac aaggaaaaaa tgctcgaagc tattccggct atccaaccgg ttgccaataa
     2641 agatcttacc cgtgtggcat cgggctacgg tatgcgtatg gatcccattt atcattcacg
     2701 ccggtttcat gccgggatgg acttcacggc acccacggga acggatatct acgccaccgg
     2761 caacgggcgg gtgctgagtg ccggctggca gcagggttat ggcaactgtg tggaaattga
     2821 tcatggattt ggttatgtaa cactatatgg acacatgcat tctatttctg taaatgccgg
     2881 acaaactgtg aacagaggcg aagtgatcgg tctggtaggc aatacaggta aatccaccgg
     2941 cccacacctg cactacgagg tgcactacaa aggcgaaatc atgaatccac agaattatta
     3001 tttcctggat ttgacacccg aagaatatga caaaatggtg cagttgagca acaatgccgg
//
LOCUS       DAOPZK010000197         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_44862_length_3014_cov_22.820210, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000197 DAOPZK010000000
VERSION     DAOPZK010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44862_length_3014_cov_22.820210"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="PKH68_08840"
     CDS             <1..1752
                     /locus_tag="PKH68_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13082.1"
                     /translation="FSTMNIPINEFEQHIDEDILKRGLQYFKKGHVTYVDELSAGEYE
                     ATVEGTETYTVHLQLKNGSVIANRCTCPYDWGPFCKHEVAVMFYLQQEELGLKVKKKS
                     TPKSLPLTDTPKTEKKTVKKKKTIQEQVEEILDKLSADELKTYIGEFCKKDKAFRDLF
                     LAQNLHLVQPVSQEMYARQIQAIVRSVAGRHGFIDYAGARQLGNAIHQLFQLAEKAIT
                     AGNWREAMFMGCAILEEMTKAIEQGDDSNGDLGDCIGNAQEILFRIAQECPDSAVCRE
                     LFEYAIDVYRRQLFEGWDWHHEMLELAVELQETEDQKKQIIQLIDQIKPKNDGWNYRY
                     EENQKLRLKLIRKTEGETQAIEYLEANITNSDFRRELILKAIKEKNYTKAISLAEKGI
                     AADQRDKPGLADEWHWHLMNIYQMQGEKEKVIEKTRYLFLHTGRFSSKEMYDILKKNI
                     PAGDWPAFFEQLITEKIKSERWVSFHQIADIYSWEEQWENILNLLNKYPTLDNIAHVE
                     KQLATRYAPQLIDLYFREIQLFLKNNISRSHYQTACKYLRRMKKLGGQDKTEKLIAFL
                     RFTYPQRRALMEELNRV"
     gene            2176..>3014
                     /locus_tag="PKH68_08845"
     CDS             2176..>3014
                     /locus_tag="PKH68_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13083.1"
                     /translation="MITFLGAIKTNSNTNFKKQDMARVKNLDQITGTLKNLSFYTRKG
                     SDAIFVRTKGGPSKEKIKRDPAFEGMRRHNREFGGCSAMSKQIRQAFSGLEHVADYNL
                     SAALSSLMNSIQKTDDAHEKGSRDICLSHYRYTLAGFDFGRKRRFSSLLRVPLSYTID
                     REKQAATVLIPSFACSFGLNLNADYMQGAVNSGTFRFTAALGIVTDMKLNKSGTAYEP
                     VHDKLGQGYQTRSTAWYSTQGSVPGQELRLELLNEDLATMSDPLTSPPFIPEFTDADS
                     LVLT"
BASE COUNT          954 a          637 c          693 g          730 t
ORIGIN      
        1 ttctcaacca tgaatattcc tatcaatgaa tttgagcaac acatcgacga ggacatcctg
       61 aaacgcggac ttcaatactt caaaaaagga catgtgacgt atgtagatga actgagcgcc
      121 ggagaatacg aagctactgt ggaaggcact gaaacctata ccgtacattt acaactgaaa
      181 aatggaagtg taatagccaa tcgttgtacc tgtccttacg attgggggcc tttttgcaag
      241 cacgaagtgg ctgtaatgtt ctatctgcag caggaagaac tcgggctcaa agtgaaaaag
      301 aaatctaccc cgaaatcctt acccctgacc gatactccca aaacagagaa aaaaactgtc
      361 aaaaagaaga aaaccataca ggaacaagtg gaggagatac tggataaatt atctgctgat
      421 gagctgaaaa cgtatattgg cgagttttgc aaaaaagaca aagcgtttcg cgatctcttt
      481 ctggcgcaga atttacatct cgttcagccc gtatcccagg aaatgtatgc ccggcaaatt
      541 caagccatcg tacgttcggt agccggaaga cacggtttca tagattatgc cggagcccgt
      601 cagttgggca atgcaattca tcaattgttt caactggcag aaaaagcaat tacagccggc
      661 aattggcgtg aagctatgtt catggggtgt gctatactgg aggaaatgac caaagcgata
      721 gaacaaggcg atgacagcaa cggtgacctg ggcgattgca taggaaatgc gcaggagatt
      781 ttgtttcgta tagcgcagga atgtcctgac agtgctgttt gccgggaact gttcgaatac
      841 gcaatcgatg tgtaccggcg tcaacttttc gaaggatggg actggcatca cgaaatgctc
      901 gagttagctg tggagctgca ggaaacggaa gatcaaaaga aacaaatcat tcaactgatt
      961 gaccagataa aaccaaaaaa tgacggttgg aattatcgct atgaagaaaa tcagaaactc
     1021 aggctgaagc taatccggaa aacggaagga gaaacacagg ccattgagta cctcgaagcc
     1081 aatatcacca actcagactt ccggcgcgaa ctgatactga aagccattaa agaaaaaaac
     1141 tataccaaag ctatctcttt ggctgagaaa ggaattgcag ccgatcaacg ggataaaccc
     1201 gggttggcag atgaatggca ctggcacctg atgaacatct atcaaatgca gggagaaaaa
     1261 gaaaaagtga tagaaaagac ccggtattta ttcttgcata ccggacgttt cagttccaag
     1321 gaaatgtatg acattctgaa aaaaaatata cctgccggtg attggcctgc atttttcgaa
     1381 caattaatta ccgaaaagat taaatccgaa cgatgggttt cgtttcatca aattgccgac
     1441 atatacagct gggaagaaca gtgggaaaat atcctcaatt tgctgaataa ataccccaca
     1501 cttgacaata tcgcccatgt ggaaaaacaa ctcgccaccc ggtatgcccc tcaacttatt
     1561 gacctttatt tcagggaaat tcagttgttt ttgaaaaaca acattagccg cagccattat
     1621 cagactgcct gtaaatacct ccggcgtatg aaaaaactgg gcggacagga taaaacagaa
     1681 aaactaatcg cttttctgcg cttcacctat ccgcaacgac gggcgctgat ggaggagctg
     1741 aatagagtgt agaggaataa gtgaatgatt tattcctccg gcaaaagttg gagggaattc
     1801 ataaattgga ttggacgcca tttattccgc atggagggag cccattcata acaataataa
     1861 attcaaactg gggctttagc caaattactc aaatttagat ttcaatcttt tcttccaaaa
     1921 aacggcgtcg ttgtattcac cttcacattt tttcggagta gatataattt tttcaatccc
     1981 caacctctcg tagttaccaa acaggtatca tgttattgtc atatagttaa atgtaatgga
     2041 tatatattgg atatgtaatg gatatataat ggatatattt tggtaaaaag aactaaaata
     2101 tatgcttttt atatctgtaa tataattttt atatctatat ttgtaataac tatctggaga
     2161 ctatcagcac cttgcatgat aactttcctg ggagctataa aaacaaattc aaatacaaat
     2221 tttaaaaaac aggatatggc acgggtaaaa aatctggatc agataacggg cacgctgaaa
     2281 aacctgtcgt tttacacccg caagggaagc gatgcaatct ttgttcgcac caaaggcgga
     2341 ccatccaaag aaaaaatcaa gcgcgatccg gcattcgagg gaatgcggcg acacaaccgc
     2401 gaatttgggg gatgctctgc catgagcaag cagatacggc aggcattcag cggactggaa
     2461 cacgtggccg attacaacct ctcagcagca ctgagttcgc taatgaatag catacagaaa
     2521 accgatgacg ctcacgaaaa aggcagccgc gatatatgct tgtcgcatta ccggtacacg
     2581 ctggcagggt ttgatttcgg gcgcaaaagg cgtttcagtt cgctgttgcg ggtgccgcta
     2641 agttatacca tcgaccggga gaaacaggct gccacggttc tcattccttc gtttgcgtgc
     2701 agcttcggcc ttaatctgaa tgctgactat atgcagggag ctgttaactc cggtacgttt
     2761 cgttttaccg ctgcgctggg tatcgtaacc gatatgaagc tgaataagtc cggcacagcc
     2821 tacgaaccgg ttcacgataa gctgggacag ggttaccaga cccggagtac agcgtggtac
     2881 agcactcagg gcagtgtgcc cgggcaggaa ttgcgacttg agctgctgaa tgaggattta
     2941 gctacaatga gtgacccgct cacttcgcca ccctttattc cggaattcac cgatgccgac
     3001 agcctggtgt tgac
//
LOCUS       DAOPZK010000198         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_45006_length_3008_cov_19.085676, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000198 DAOPZK010000000
VERSION     DAOPZK010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45006_length_3008_cov_19.085676"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1856
                     /locus_tag="PKH68_08850"
     CDS             153..1856
                     /locus_tag="PKH68_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNX13084.1"
                     /translation="MSNYYKDNDALKFQLSHPLMKKIVALKERNYADKEKYDIAPRDF
                     EDAIDNYDKVLEIIGEISGEIIAPNAEGVDKEGPQVVNNRVIYASGTQQNQDALTQAG
                     VYGMALPRQFGGLNFSMLPYVMSGEIVSRADAGFANIWGLQDCAETIVEFADEDIKAE
                     FLPRVNMGDTCSMDLTEPDAGSDLQAVQLKATEIDGQWYLNGVKRFITNGDAQIKLVL
                     ARSEDGTNDARGLSYFVADNKHDNTVKVRRIENKLGIKGSPTCELVFTNTPAKLVGTR
                     RLGLIKYVMSLMNGARLGVGAQSVGVSEAAYQEAVAYANDRKQFGKAIVEFPAVYEML
                     SLMKAKLDASRSLLYETTRFVDMYKAYEGVENERKLTPEEKAEYKEYQKLADAFTPLV
                     KMFASEYANQNAYDSVQVHGGSGFMKDYACERIYRDARILTIYEGTTQLQVVAAIRHV
                     TTGTYLKQIREYEKQAINPEFYSFRKRLMVMTNLLENSVARVDEIKNQEYLDFQARRL
                     VEMAGHIIMGYILIIDASRNEMFRKSAEVYLNYSEVEVRKHAAFIKTFNVESLDRYRM
                     A"
     gene            2546..>3008
                     /locus_tag="PKH68_08855"
     CDS             2546..>3008
                     /locus_tag="PKH68_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13085.1"
                     /translation="MKKIVFLVCMSLLFSMNMSAQGWLEKVGQKAVNTAKQKVENRAA
                     KKAGEATDKALDKLEKKDKKNVETEENKDAGVQTADKSVQEEKLTATSKYDFIPGEKV
                     IFFDDFSQDAVGDFPALWNTNGSGEVVTTNLFPGNWMKLNSECCVWTDKLLT"
BASE COUNT          990 a          596 c          646 g          776 t
ORIGIN      
        1 gactatgtaa tcaccggaaa tgtggaagat gtagtaccca aaatgataaa atactacaaa
       61 tccaacagta aataaatatt gtttttcaat aaaaaaccag tgtctctgcc aatccactaa
      121 ccggatagtc ggagcagaaa aagaaaaaga ttatgagtaa ttattataaa gacaatgacg
      181 cactgaaatt tcagctttct catccgctga tgaaaaaaat agttgccctc aaggagcgta
      241 attatgctga taaggaaaaa tatgatatcg ctccgcgcga ttttgaagat gccattgaca
      301 attacgataa agtattggaa attatcggcg aaattagcgg tgaaatcatt gctccaaatg
      361 ccgaaggagt ggacaaggaa ggccctcagg tagttaacaa tcgtgtaata tatgccagtg
      421 gcacccaaca aaaccaggac gctctgactc aggccggagt gtatggtatg gcattgccac
      481 gccagttcgg tgggttgaat ttttccatgt tgccctatgt gatgagcggc gaaattgttt
      541 cgcgtgccga tgccggattt gccaatatct ggggacttca ggactgtgcc gaaacgattg
      601 tagaatttgc cgacgaagat attaaggctg aatttttgcc aagggtgaac atgggcgata
      661 cctgctccat ggacctcaca gaacctgatg ccggatccga ccttcaggcc gtacagctca
      721 aagctaccga aatagacgga caatggtatc tgaatggtgt aaaaagattt attaccaatg
      781 gcgatgccca gataaaactg gtattggcac gctccgaaga tggaaccaac gatgcccgcg
      841 gactatctta ttttgtagct gacaacaagc acgacaatac tgtaaaagtt cgccggatag
      901 aaaacaagct gggtataaaa ggatcgccca cctgtgaact tgtatttacc aacaccccgg
      961 ctaaattagt gggaacccgc cgcctggggc tcatcaagta tgtgatgtcg cttatgaatg
     1021 gcgcccgcct gggagtggga gcacagtcgg taggtgtttc cgaagcagcc tatcaggagg
     1081 cggtggctta tgccaacgac cgtaagcagt ttggaaaagc aatcgttgag tttcccgctg
     1141 tgtacgaaat gctgtcgctc atgaaggcca aactggatgc ttccagatcg ttgctttacg
     1201 agacaacccg ttttgtggat atgtacaaag cctacgaagg tgtggaaaac gaacgcaaac
     1261 ttactcccga agaaaaagcc gaatacaagg aatatcagaa actggccgat gcatttactc
     1321 cgctggtgaa gatgtttgcc agtgaatatg ccaaccagaa tgcatacgac agcgttcagg
     1381 ttcatggtgg ttcgggcttt atgaaagatt atgcctgcga acgaatctat cgcgatgcac
     1441 gtatcctcac catttacgag ggcacaaccc agcttcaggt cgttgcggct atccgccatg
     1501 tgactaccgg tacttatctg aagcaaatca gggaatatga aaaacaggcc atcaatcccg
     1561 aattctattc attccgaaaa agattgatgg taatgaccaa tttgctcgag aactcagtag
     1621 cacgcgtgga tgagatcaaa aatcaggaat acctggactt ccaggcacgc cgcctggtgg
     1681 aaatggcagg tcacatcatc atggggtata tccttataat cgatgccagc cgcaatgaaa
     1741 tgttccgtaa atcggctgaa gtgtatctca actactccga agtggaagta cgaaaacacg
     1801 cagcatttat caaaacattc aacgtggaaa gtctcgacag atatcgtatg gcctgataca
     1861 acatccctta ttaaataaaa aaacgctgga atttcgattt cagcgttttt tttattttca
     1921 cagagcactc catgccagta attacgacac aagaccaaaa tcagattcga aaccggatta
     1981 aacgaatatt tattacctga aagctcgctg tattatttga aatcaatatt cgattatttg
     2041 taatctaaat cgataaatta gggtctgctg aaaaatgctt atcaagttat aaatcaaata
     2101 attatattgg tataaattat tcttaatgca tgcattttga ataaataaca caatattatt
     2161 tgcatcatgt ttcggtgctc tgcaccttaa tattttatct tcaataaatt tactacaaat
     2221 atcacggtgc tctgcaccta aatttaagcc acagagtagc gtaacatttg tagaaaataa
     2281 attattctcg aattataagg tacagagtac cgaaacattt tgtagttgag tttttcagca
     2341 gaccctaatt aaagaagaaa aaaaataccc attaccgaac aaaaatttaa ctgatacaca
     2401 aaacaatacc tgctttttgt atgttattct atgtagttat ataaataaaa ttaatcttaa
     2461 aagttgtagc tatcaactta aacattccct ttccacacgt gcaggatttt catcagaaat
     2521 tgtataattt ttaaaaataa ataccatgaa aaaaattgta ttcttagtat gtatgagtct
     2581 gttgttcagc atgaatatga gtgctcaggg atggctcgaa aaagttggtc aaaaagcggt
     2641 aaacaccgcc aagcaaaaag tggagaaccg cgcagccaaa aaagccggcg aagcaaccga
     2701 caaggcgctc gataaactcg aaaaaaaaga caaaaagaat gtagaaaccg aagagaataa
     2761 agatgcgggt gtacagacgg ctgataaaag tgtgcaggaa gagaaactga cagcaacatc
     2821 gaagtatgat tttattcccg gtgaaaaagt aattttcttt gatgatttca gtcaggatgc
     2881 tgtgggcgat ttccctgcac tctggaatac caacggatct ggtgaagtgg ttacaaccaa
     2941 tttgtttccg ggaaactgga tgaaacttaa ctcggaatgc tgtgtatgga ctgacaaact
     3001 tttaactc
//
LOCUS       DAOPZK010000199         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_46400_length_2948_cov_20.146906, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000199 DAOPZK010000000
VERSION     DAOPZK010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46400_length_2948_cov_20.146906"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..2893
                     /locus_tag="PKH68_08860"
     CDS             98..2893
                     /locus_tag="PKH68_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HNX13086.1"
                     /translation="MNKRIALLALFALLGYEAAHLYGQTTSASSYFAAQPIPDRTVTF
                     NLSDPGVSKPIIWGLDLAWLSESNIRRGIAFMGADKVDVVRASFQPTYPLINGDLQTA
                     QITDLNTRLTLIDLTGPTTKVALNCDHPNVDAWYIANAVNWATLIDATTRRVQERGRT
                     VVSVAPFNEPDYGWGQYSGQNGQLDFYNIAGELRKNPRFDGIRISGGNTLNCDQALPW
                     YNYLKNRLDEGNTHQLAGNFDNYANFYRTLKTNGHHGTNDELHNVMEAMVGVEYGMQT
                     GIWWGTAEYARGEFVKASDGVRIGYAEHRPNWTAASVYRSPEGKIQAFGGTSERQAVT
                     TTYRFLSKEMDVYYDGYGPQREYTMVLPGGTGYQNGQTNAERVVSITTGDDIQPVING
                     RYVLVNRNSGKVMEVAGGSVTTGANVRQNSVIAGATFQQWNVTPVDSRIGGDFSYFTI
                     TAVHSGKSLDILNWSLYNGGNILVWDDAKGANQQWYLEYAGDGWFYIRSRHSAKCIEV
                     ANASTANEANIYQWGKDGDYNQQWRFIPVDAPVEFVKPGVPAGLMASPNAESVRLDWT
                     ASPDGDVAGYHVYRKDTPEGNYQTIARNVKGTSFVDNTTTLSGQYFYAVKAVDYSLNR
                     SDYSNEASAITTGADDLVTRLQFENNTADSTINLNHSATYGTISYVGGKVGNNAISLN
                     GSNSFVQLPSNLANQQEITIATWIYWKGGGAWQRIFDFGNSQSEYMFLTPNNGSSQLR
                     FSIKNGGAEQQLNATALVTAKWNHVAVTLAAGAARMYVNGVMVSESNSITISPMDFKP
                     VLNYIGRSQFSDPLLNGYIDDFRVYNYALTANEIAEIPGLLSGIEDTEYVSEKKLSVY
                     PIPAKNILNVKYQGEINNQKTRIWVYDTNGRLLISVDITDTNETKLNVSEFPQGVYLL
                     KLTNNAETLVKRFVVNH"
BASE COUNT          872 a          582 c          690 g          804 t
ORIGIN      
        1 atttacaacc cttttttgac aaattaattc tctgtcatta aattaaatat acttacattt
       61 gtcaatcaaa tttactgaaa aatcagacag aaaataaatg aataagagaa ttgccctgct
      121 tgccttattt gccttattgg gatatgaagc agctcatcta tatggtcaga ccacaagtgc
      181 aagcagttat ttcgctgctc aaccgatacc tgatcgcact gtcacattca atttatcaga
      241 cccgggtgtg agtaaaccga tcatttgggg attggatttg gcctggttga gcgaatccaa
      301 tatccgacgc ggcatagctt ttatgggtgc cgataaagtt gacgtagtca gagcatcttt
      361 tcagcctact tatcccttaa tcaatggtga cctgcaaact gcacaaataa cagacctgaa
      421 cacccggctt acgctgattg atttaaccgg accaactacc aaagtagctt tgaattgcga
      481 tcatcccaat gtagatgcct ggtacattgc caatgcggta aactgggcaa cactgattga
      541 tgctacaacc agacgtgtgc aggaacgtgg tcgtacagtg gtatcagttg cccctttcaa
      601 cgaaccggat tacggctggg ggcagtattc cggccaaaac ggtcaactcg atttctataa
      661 tattgccgga gagttgagaa aaaatccccg atttgatggt attcggattt caggaggcaa
      721 tacacttaac tgtgatcagg ctttaccctg gtacaactat ctgaaaaaca ggctggatga
      781 aggaaatacg catcaacttg ccggaaactt cgacaattat gcaaactttt acagaacctt
      841 aaaaaccaac ggtcatcacg gtacgaatga tgaactgcac aatgtgatgg aagctatggt
      901 gggggtggaa tatggcatgc agaccggtat ctggtgggga acggccgaat acgcccgggg
      961 agaatttgta aaagcaagtg atggtgtcag gataggttat gctgagcatc gtcccaactg
     1021 gactgctgcg tctgtttatc ggagtcctga aggaaaaatt caggctttcg gaggtacatc
     1081 agagcggcag gcggttacta caacgtatcg tttcctgtca aaagagatgg atgtgtatta
     1141 tgatggatac ggacctcagc gtgaatatac gatggttctt ccgggtggca ccggatacca
     1201 aaacggacaa acaaatgccg aaagagtagt gagtattact accggtgatg atattcaacc
     1261 ggttattaac ggtcgatatg ttttagtaaa ccgtaatagt ggaaaagtca tggaagttgc
     1321 cggaggatct gttaccactg gagctaatgt tcgccaaaac agtgttattg cgggagctac
     1381 ttttcagcaa tggaatgtaa cgcctgtgga ttcgagaatt ggaggtgatt ttagttattt
     1441 caccattact gccgttcata gtggaaaatc gttggatata ttaaactggt cgctatataa
     1501 tggcggaaat attttagtgt gggacgatgc taaaggtgca aatcagcaat ggtatctcga
     1561 atatgccggt gacgggtggt tttatatccg cagccgtcat agcgctaaat gcattgaagt
     1621 ggccaatgca agtactgcca acgaagcgaa catttatcag tggggaaagg atggcgatta
     1681 taatcagcag tggaggttta ttccggttga tgcacccgtt gaatttgtga aacccggagt
     1741 accggccggt ttaatggcct ctcccaatgc cgagtctgta cggcttgact ggacagccag
     1801 tccggatggt gatgtagcgg gttaccatgt ttatcgaaaa gatacacctg aaggcaatta
     1861 tcagacaatt gcccggaatg tgaaaggtac atcatttgtc gataacacta ctacgctgag
     1921 cggacagtat ttctatgccg ttaaagcggt ggattattcg ttgaaccgtt ccgattattc
     1981 caatgaagct tcggcaataa ctaccggagc tgacgatttg gttacgcgcc ttcagtttga
     2041 aaataatact gccgacagca caattaatct aaatcacagc gcaacctacg gcactatctc
     2101 ttacgtaggt ggcaaagttg gcaacaatgc tatttcgctg aatggttcaa actcattcgt
     2161 acaacttcca tctaatctgg caaatcagca ggaaatcact attgccacat ggatttactg
     2221 gaaaggtggt ggcgcctggc agcgaatttt tgattttgga aactctcagt ctgaatatat
     2281 gtttttgact ccgaataacg gtagtagtca actgcgcttt tcaataaaaa acggaggtgc
     2341 tgaacagcaa ttgaatgcta cagctctcgt taccgccaaa tggaatcatg tagcggtaac
     2401 ccttgctgca ggtgcagctc gcatgtatgt gaacggagtg atggtcagtg aatcaaattc
     2461 gattactatc agcccgatgg atttcaaacc ggtattaaat tacataggcc ggagccaatt
     2521 ttcagatccg ctgctcaatg gttatattga tgatttcagg gtgtataatt atgctttgac
     2581 tgctaatgaa attgccgaaa ttccggggct tctttccgga attgaagata cggaatatgt
     2641 aagcgaaaaa aaattatccg tctatcccat accggctaag aatattttga atgtaaaata
     2701 tcagggtgaa atcaataacc aaaagacccg aatatgggtg tatgatacaa acggtagatt
     2761 gttgattagc gtagatatta ctgatacaaa cgaaactaaa ttgaatgtat cggaatttcc
     2821 acagggtgtt tacctgctta aactgaccaa caacgctgaa acactcgtta aaagatttgt
     2881 tgtaaatcat taaccccggg gtaatgattc ttttagaaaa taaaaaagta tgtgcgctct
     2941 ttttgcac
//
LOCUS       DAOPZK010000200         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_47839_length_2890_cov_24.461376, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000200 DAOPZK010000000
VERSION     DAOPZK010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47839_length_2890_cov_24.461376"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKH68_08865"
     CDS             <1..612
                     /locus_tag="PKH68_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNX13087.1"
                     /translation="KKIIDALYFCLLSVIYMLFSSAKIDFRAPIPVGKPVIFVANHQS
                     LFDIMTMGWFLRRYHPKFVSKIELGRNIPSVSYNLRHGGSVLIDRKNPRQALPALKQM
                     GEYIQKHNRSVVIYPEGTRSRDGRPGHFSNNGLKILCKFAPDALVVPVTINDSWRVFR
                     YGGFPFGLGTCIKLIAHEPMAVKDYDFDTLFDKTVKAIVDDIK"
     gene            complement(749..1762)
                     /locus_tag="PKH68_08870"
     CDS             complement(749..1762)
                     /locus_tag="PKH68_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNX13088.1"
                     /translation="MKDIIPAVDRVLLKAELTSKTYVRPTNKGHNEIYITTAAASPNV
                     MREIGRLRELAFRSWGGGTGNELDTDKYDFLEKPFKQLIVWDPEAEEIVGGYRFITGE
                     DVTFDSQGQPAFVMSHLFHFSDRFIRNYLPYTIELGRAFVQPDYQTAKMGTKSLFALD
                     NLWDGLGALIHQNKKYLYFVGKVTVFDTYPLLARELLYQYMFLHCPDPDELVRPKLEV
                     QVSEEGKLKALEIFTGSDAREDYKALQKAIRHEGTTIPPMFNAYIGLTDTLRMFGSMI
                     DPDFSGVYESGIMLTIGDLIESKRQRYIDPYIDYLRQLVNEKRAARRAAKIAKEKEAN
                     RRK"
     gene            2310..>2890
                     /locus_tag="PKH68_08875"
     CDS             2310..>2890
                     /locus_tag="PKH68_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNX13089.1"
                     /translation="MKKLMLLLMISMLSASLFAQHELFQKKEFVYKGDTLRYRVLFPV
                     NYDPSQEYPLVLFLHGMGERGSDNEKQLIHGASLFADEKNRLDYPSIVLFPQCPDNDF
                     WIKIPLRQSSTIDFPAKAPVTKPLLLVKKLVDSYVKKEAVDKKRIYVAGLSMGGMGTF
                     DLICRYPKYFAAAMPICGGVNVDRLKKVKKMPIR"
BASE COUNT          769 a          617 c          644 g          860 t
ORIGIN      
        1 aagaaaatca ttgatgcctt gtatttctgc ctgctgagtg tgatatatat gcttttctca
       61 tcggctaaaa ttgattttcg ggctcccata ccggtaggaa aaccggttat ctttgtggcc
      121 aatcatcaga gtttgttcga tatcatgacc atgggctggt ttctacgtcg ctatcacccc
      181 aagtttgtta gcaaaataga gctgggacgc aatatcccga gtgtatcgta caacctgcgc
      241 cacggtggtt cggtgctaat cgaccgtaaa aacccgcgcc aggccttgcc tgccctcaaa
      301 caaatgggcg aatatattca gaagcataac cggtcggtgg ttatctatcc cgaaggtacc
      361 cgctcgcgtg atggccgacc gggacatttc tccaacaacg ggctgaaaat tttgtgtaaa
      421 ttcgctcccg atgcacttgt ggttcctgta acaatcaatg attcgtggcg tgtattccgg
      481 tatggcggtt ttccgttcgg acttggaacc tgcattaaac tgatagccca cgaaccaatg
      541 gctgtgaagg attatgactt tgatacgttg tttgataaaa cagtaaaagc cattgtggac
      601 gatataaaat agagctgaaa agcagtaaaa gcaattaaaa agtggtcgtt aagttttacg
      661 ggagtattcg tagaattagc gattcataat aaagggtggt taaattaatt gtatcgcttt
      721 ttttgcatcc tcttcccgga aaacttaatc attttcgacg attagcttct ttttctttgg
      781 caatttttgc agcacggcgt gctgcccgct tttcgtttac aagctgtcgg agataatcta
      841 tataggggtc aatatagcgc tgccttttcg actcgataag gtcgccgatg gtgagcatga
      901 ttccgctttc gtacaccccg ctgaaatcgg gatcaatcat cgaaccgaac atgcgcagtg
      961 tatcggtcaa cccgatatag gcattgaaca tggggggaat ggtggttcct tcgtgacgaa
     1021 tagctttctg gagtgctttg taatcctccc gcgcgtcgct tccggtgaat atttccaggg
     1081 cttttagctt tccttcttca cttacctgta cttccagttt gggacgtacc agttcatccg
     1141 ggtcggggca gtgcagaaac atatactgat agagcagctc tcgggcaagc agcggatagg
     1201 tgtcgaacac cgtaactttt cccacaaagt agaggtattt cttattttgg tggatcaatg
     1261 cacccagccc gtcccacagg ttgtcgagcg caaagaggct tttggtgccc atttttgccg
     1321 tttgatagtc gggctgcacg aatgcgcgtc ccagctcgat ggtgtaaggc agatagttgc
     1381 gaataaaccg gtcgctgaag tgaaacagat gcgacatcac aaaagccggc tgtccctgtg
     1441 aatcaaaggt tacatcttct cctgttataa aacgatatcc tcccactatt tcttcggctt
     1501 cggggtccca cacaatgagt tgtttaaatg gtttttccag aaagtcgtac ttgtcggtat
     1561 ccagttcgtt gccggtgccg cctccccacg agcgaaacgc cagttcacgc aatcgcccga
     1621 tctcacgcat aacattgggt gatgcagcag cggtagtgat gtatatctcg ttgtgacctt
     1681 tattggtcgg tcttacatac gttttggaag tcagttccgc tttcagtagt actctatcga
     1741 cggctggtat tatgtccttc atactgtttt atttcaactt gatagttttg ggaggattac
     1801 ttcgtgcaaa attaataaaa aatcgttaat ggctcagaaa aataatatat cagtgtaaca
     1861 aattcaccga atagtggcgt gagctttcag tggtactttg gtataacaaa caaaaagtaa
     1921 tttttcctca cacgcttaaa aaatttatag atgacggttc atactggtgg aaaaatgttt
     1981 gaatatcaat cagtctgcat cagtattacc tccggcgatg cgtctgtgaa gggtttcagg
     2041 ttaaaaatat taaaaaatca actttctcat cgtaattttt tctgtgttat tttgttggtt
     2101 ttcaaggctg ttttgattta aaaataaaat ctttcaaagt tatttcggtg aatgtttaat
     2161 aatctgcgca taacatgaat aaaatttgac cttaatggtc agaaaatcat tttttaataa
     2221 tatggaatat tcgctacatt tgccaaacat taccgtatta attgcgttat tacctactaa
     2281 acatctaatc acagtttaaa tacaatgcca tgaagaaatt aatgttgtta ctgatgatat
     2341 ccatgttgag tgcctcactc tttgctcagc atgaactttt ccagaaaaag gaatttgttt
     2401 ataaaggtga tacgctgcga tatagagtac tctttccggt caattacgac ccttcacaag
     2461 aatatccgct ggtactgttt ctgcatggga tgggcgagcg gggcagcgat aacgagaagc
     2521 aactcataca cggtgcttcc ttattcgccg atgaaaaaaa ccggctcgat tacccttcca
     2581 ttgtactttt cccccagtgt cccgataatg atttctggat taaaatccct ctccgtcaaa
     2641 gcagcaccat cgattttccg gctaaagcgc ctgtcaccaa accgttgttg ttggtcaaaa
     2701 agctggtgga cagctatgtg aaaaaggaag cagtggacaa aaaacggata tatgttgccg
     2761 gactgtcgat gggaggcatg ggcacattcg accttatctg ccgttacccg aagtattttg
     2821 cagcagccat gcctatttgc ggtggagtga atgtggatcg cctcaaaaaa gtaaagaaaa
     2881 tgcccatccg
//
LOCUS       DAOPZK010000201         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_47941_length_2886_cov_24.551042, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000201 DAOPZK010000000
VERSION     DAOPZK010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47941_length_2886_cov_24.551042"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..1242)
                     /locus_tag="PKH68_08880"
     CDS             complement(373..1242)
                     /locus_tag="PKH68_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNX13090.1"
                     /translation="MKHFPPSELIINDDGSIFHLHLKPEQISDKIILVGDPGRVALVA
                     SYFDEQECSVSSREFNTITGTYKGKRLTVISTGIGTDNIDIVVNELDALANIDLIDRH
                     EKPDFKQLTLVRIGTSGGMQPEIPLGSFLISEKSIGFDGMLNFYAGRDGVCDLEFEEE
                     LKKQLDWNKQFCAPYVVNADAELTDRIGRDDMLRGVTISANGFYGPQGRELRIGLADP
                     RLNDKIEEFRFDNYKITNYEMEGSAIAGLARLMGHKALTVCCIIANRRVEAANTDYKP
                     YIEKLVQTVLERI"
     gene            complement(1342..1710)
                     /locus_tag="PKH68_08885"
     CDS             complement(1342..1710)
                     /locus_tag="PKH68_08885"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNX13091.1"
                     /translation="MKNEAVIMLGSNTDISLLLSAKSMLEDWFDVVAVSEIMQTTAIG
                     EQYQSPFYNQAIRIQTEYDREETIRIFKKTEKKLGRTPESKTEGIVPIDIDLIVWNGE
                     ICHDDYYRFDFVKKCVDEIT"
     gene            complement(1740..1913)
                     /locus_tag="PKH68_08890"
     CDS             complement(1740..1913)
                     /locus_tag="PKH68_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNX13092.1"
                     /translation="MPAESKNKTFVLRIDTETMEAIEKWAADEFRSTNGQILYLLDQA
                     LTKSGRKPKKTTS"
     gene            complement(1921..>2886)
                     /locus_tag="PKH68_08895"
     CDS             complement(1921..>2886)
                     /locus_tag="PKH68_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNX13093.1"
                     /translation="MKTKEEVFTGFKFPGIPAIFLNILLLAGIVLLFIWGVTSSLSNN
                     LAVFVIILSVLLFICNLIAWGGFMQIEPNEARVMIFFGKYEGTVKENGFFWVNPFYTK
                     KRLTLRARNLDAEPIKVNDKAGNPIMIGMVVVWSVVDTYKASFDVDNASMTSGVPGTN
                     QDNVNAKMKAYENFVKIQSDAALRKVAGMYAYDHNTSGDETSLTLRSGGEEMNELLEN
                     QINERLRIAGIEVIEARINYIAYAPEIASVMLRRQQADAIIAAREKIVEGAVGMVKMA
                     LDKLSKDGIIELDDDKKAAMVSNLLVVLCGESNAQPVINSGTLYN"
BASE COUNT          763 a          638 c          590 g          895 t
ORIGIN      
        1 cctgaggtga aactgaattc gctgagtttt gattatttat atccatttca tctttttctt
       61 aaagtccaca gttgatgtaa ataaaaatat tgagaataaa aggctaattt agatcattgt
      121 tgtccgataa aaatacagaa acgtctgtaa tttactgtaa tataaacaaa aaagaatatt
      181 aagccccgcc ggggcttgct tttctgtagt taccaaatcg ctacaaatat attgcaacgc
      241 tgttgcaaaa acagtcacag atggaacatt ccgatctatc ggaagtggct aaatctttgt
      301 agtaagatgt gaaaaataag atttcttgcc acagagtggc aatatattaa gttttatcgg
      361 tcaacattga ttttaaatcc tttcaagtac cgtttgaacc agcttttcga tgtagggttt
      421 gtaatcggta ttggcagctt ctacccgacg gttggctatt atgcagcata ctgtcagggc
      481 tttgtgaccc atcaggcggg ccagtccggc aatggccgat ccttccattt cgtagttggt
      541 gattttatag ttgtcgaaac ggaattcttc gattttatca ttcaaacgag ggtcagccag
      601 cccgatacgc agttcacgtc cctgcggacc ataaaatccg ttggccgaaa tagtcacacc
      661 ccgaagcata tcgtcacgtc cgatccggtc agtgagttca gcatcagcat tgactacata
      721 aggagcacaa aactgtttgt tccaatccag ctgttttttt aattcctctt caaattcaag
      781 gtcacaaact ccatcgcgtc cggcgtagaa gttgagcatg ccatcaaatc cgattgattt
      841 ttcagaaatg agaaagctgc ccaggggaat ttcgggttgc atgccgcccg atgtgccaat
      901 cctaaccagg gtgagctgct tgaaatccgg tttttcatga cggtcaatca aatcgatatt
      961 ggccagagca tcgagttcat tcactacaat atcaatgttg tcggtgccaa ttcctgtgga
     1021 aataactgtc aggcgttttc ctttgtaagt gccggttatg gtattgaatt ctcggcttga
     1081 taccgagcat tcctgttcgt cgaaatagga tgcaaccaaa gccacacgtc ccggatcacc
     1141 gacaagtatt attttatcgg aaatttgttc gggtttcagg tgcaggtgaa atatgctgcc
     1201 gtcgtcattg atgatgagct cggaaggtgg aaaatgtttc atatcggttt ttgttttttt
     1261 aaaaatgaaa attcattata aatgaagcga accacaaata taatgaattt tttaatgata
     1321 atgtttcccg aacaatactt tctatgttat ttcatccaca cactttttta caaaatcaaa
     1381 gcggtaataa tcatcgtggc agatttcgcc gttccaaaca attaaatcga tgtcgattgg
     1441 tactatacct tctgttttac tttcgggtgt tctgcccagt ttcttttcgg ttttcttgaa
     1501 aatccggatt gtttcctccc gatcatattc tgtttgaatt cgtatagcct gattgtaaaa
     1561 gggactttgg tactgctcac cgatggcggt tgtttgcatt atttcactta cggcaaccac
     1621 atcaaaccaa tcttcgagca tcgatttggc cgaaagcaac agggaaatat ctgtgttgct
     1681 gcccagcatg ataacagctt catttttcat cggcttaggt tattgtataa taaatagtat
     1741 cacgatgtcg tctttttcgg ctttcgtccg cttttcgtca gtgcctgatc gagcaggtac
     1801 agaatctgtc cgttggtgct ccggaactca tcggcagccc atttctcgat agcttccatc
     1861 gtttcggtgt caatgcgcag aacaaaggtt ttatttttgc tttcagcagg catgtttcaa
     1921 ttagttatac agagttcccg aattaattac cggttgagcg tttgattctc cgcaaagtac
     1981 caccagcaga ttgctcacca tggccgcttt tttgtcgtcg tccagttcga taattccatc
     2041 ttttgaaagt ttgtccaatg ccattttcac catccctaca gcaccttcca ctattttttc
     2101 gcgggcagca atgatggcat cggcctgttg gcggcgaagc atcaccgaag ctatttcggg
     2161 tgcataagca atgtagttga tacgggcttc aatcacttct ataccggcaa ttctcaaccg
     2221 ttcgttaatc tggttttcaa gcagttcgtt catttcttca ccacccgaac gcagcgtaag
     2281 gctggtttcg tcgcccgatg tattgtggtc gtaggcatac atccctgcca ccttgcggag
     2341 tgcagcatcg ctctgaattt ttacaaagtt ttcgtaggct ttcattttag cgttcacgtt
     2401 gtcctgattg gttcccggta cgccagaggt catggatgca ttgtccacat cgaaggaagc
     2461 tttgtaggta tccaccacac tccataccac caccataccg atcatgattg gattaccggc
     2521 tttgtcgttt accttgatag gctcagcgtc caggttgcgg gcacgcagtg tcaaccgttt
     2581 cttggtgtag aatggattga cccagaaaaa gccgttttcc tttaccgttc cttcatattt
     2641 tccgaagaaa atcatcacgc gggcttcatt cggttctatt tgcataaaac ctccccacgc
     2701 aatcagattg catataaaaa gcaaaacact gagaataata acaaacacag caagattatt
     2761 acttaatgac gatgtgacac cccagataaa taacaatacg atgccggcta acagtaagat
     2821 attaagaaaa atagcaggaa tgcccgggaa tttgaaaccg gtaaatactt cttcttttgt
     2881 tttcat
//
LOCUS       DAOPZK010000202         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_49405_length_2831_cov_22.731268, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000202 DAOPZK010000000
VERSION     DAOPZK010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49405_length_2831_cov_22.731268"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..225)
                     /locus_tag="PKH68_08900"
     tRNA            complement(153..225)
                     /locus_tag="PKH68_08900"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Gly,seq:tcc)
     gene            complement(299..381)
                     /locus_tag="PKH68_08905"
     tRNA            complement(299..381)
                     /locus_tag="PKH68_08905"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Tyr,seq:gta)
     gene            complement(427..951)
                     /gene="ribH"
                     /locus_tag="PKH68_08910"
     CDS             complement(427..951)
                     /gene="ribH"
                     /locus_tag="PKH68_08910"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNX13094.1"
                     /translation="MATQYHNLSNYDPDLMPEKDIVATQKYAIAVAEWNPDITEALLD
                     GTVNTLIENGVTTNNITIVHVPGTFELTFAAKRMYEDYTINEKNEKAHKYAAIIVLGC
                     VVQGETPHFNYVCQAVTAGVTELNTRPDGCPVIFGVLTTNTLQQAKDRSGGIHGNKGD
                     EAAITAIKMANIIW"
     gene            complement(1035..1706)
                     /locus_tag="PKH68_08915"
     CDS             complement(1035..1706)
                     /locus_tag="PKH68_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNX13095.1"
                     /translation="MSKKVKEPAELETVGRALSSSEAFIEKYQKQILIGVGVIVLVVL
                     AVMAVNNFYLKPRVVAAENAMYKAQELFAVDSFKVAVDGNGKDVMGFKEIASEYGMTA
                     SGNLANAYTGICYYKLGKYQDAVKYLTQYDAGDDYFKTSVIGLTGDCYAELGETGKAQ
                     DYYSKAIGQKNELSPVYLKKAGILFETKGQAADAEKMYLQIKNDYPKSMEAGDIDKYL
                     ARVQK"
     gene            complement(2100..>2831)
                     /locus_tag="PKH68_08920"
     CDS             complement(2100..>2831)
                     /locus_tag="PKH68_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX13096.1"
                     /translation="ELKLNIYRPLKPRKLPVVLMIHGGGWSSGSPRMQQSLAVSLSRQ
                     GFATVVVEYRLSPEAIYPAAMDDLSDAVLWIYKHAREYGFDRKRIAVEGCSAGGQLAA
                     LLGTKNKDRLIRAVVNIDGISTFMDQTTIDRAEQFREKGGKMPADMQWLGGTRAEKPD
                     AWKDASAVYFVNKFSAPVCFINSSIPRFHNGRDEHVRLLKKFNIYAEVHTFEDSPHSF
                     WLFEPWHQPTVEYTSAFLKKVFEIK"
BASE COUNT          786 a          617 c          564 g          864 t
ORIGIN      
        1 accgatacga aaagagcggc taattcgcct atgtgcgatt ggagataaat cattagtagt
       61 tacaagttaa aaagttacaa gttacaagag gattgcacat taataaataa aaaaaagacc
      121 tcaacagtaa ttgttaaggt cttcgttttg agagcggaag acggggctca aacccgcgac
      181 cctcagcttg gaaggctaat gctctatcga ctgagctact tccgcaattt aaagccccct
      241 tcttattccc ccgaaggggg aaagtctccc tccctttggg agggttgggg taggcttgtg
      301 ggcagtgaag gattcgaacc tccgaagtcg aaagacaact gagttacagt cagtcccatt
      361 tggccactct ggtaactgcc cggtggtcta tcaaaagcga gtgcaaagat agctaaaata
      421 ttgggtttac caaattatat ttgccatttt tattgcagta atagccgctt catcaccttt
      481 atttccgtgt attccacccg agcgatcttt agcctgctgt aatgtattgg tggtcagcac
      541 cccaaagata acgggacatc cgtcggggcg ggtattcagt tcggtaacac cggctgttac
      601 ggcctggcaa acatagttga aatgcggagt ttcaccctga accacacaac ccagcacaat
      661 gatagctgca tatttatgtg ctttttcatt tttttcgttt attgtgtaat cttcatacat
      721 ccgtttagca gcaaaagtca gttcaaatgt tcccggtaca tgcacgattg taatattatt
      781 agtcgtaact ccgttctcaa tcagtgtatt aacagttcca tcgagcaatg cttcggtgat
      841 gtcgggattc cattcagcca cagcaatggc atatttctgt gtagctacta tatctttttc
      901 gggcatcaga tccgggtcat aattggacaa attgtggtat tgtgttgcca tattgttggg
      961 taatacttgt ttatgaatga ataaagctgc aatccggaac agaaagcagc tttataatat
     1021 ttttaatact taaattattt ttgtacgcgg gccaggtatt tgtcaatatc accggcttcc
     1081 atgcttttag ggtaatcgtt tttgatctga agatacattt tttcagcatc agcagcctga
     1141 cctttggttt cgaagaggat acctgctttt ttcagatata ccggactgag ttcatttttt
     1201 tgaccgatag ctttgctgta atagtcctga gctttaccgg tttcaccaag ttcggcatag
     1261 caatcgcccg tcagaccaat taccgatgtt ttgaaataat catctccggc gtcatattga
     1321 gtgaggtatt taactgcatc ctgatatttt cccaatttat aataacaaat accggtgtag
     1381 gcattggcca ggttaccact ggctgtcatt ccatattcag aggctatttc cttgaaaccc
     1441 atcacatctt tgccatttcc gtcgactgct accttgaatg aatcaacagc aaataattcc
     1501 tgagctttgt acatggcatt ttcggctgct acaacacgtg gcttcagata gaaattatta
     1561 actgccatca cggctaacac tacaagtaca ataacgccaa ctccaatcag aatttgtttt
     1621 tgatattttt caatgaatgc ttccgaagaa cttaaagcac gtcctactgt ttcaagttca
     1681 gcaggttctt ttactttttt agacatataa attttaggat ttgttattaa ttaattttcg
     1741 aaccacaaaa gtaaagtttt tcttttatat accgaaataa accggatgat tttttagctt
     1801 ttatttcgtc caaaaccatt atcagaagaa gtattagact aaaattaaca aaatatttaa
     1861 cgcttgaatg aacctttata ttagaaaatg tattatattt gtgaaccgca ttgttcagag
     1921 attggaaaac tcaacagaat atttatatga gtaacaacgg tttttaatgg cgggctgcgc
     1981 ccccgggtat tcgtcaattg acggacagca gattacagaa agaaccaatg cctcaaatcc
     2041 tatttatagg agttttctca acaagtaaca atgcggtttc tcgttttaaa tcccttcctt
     2101 cattttatct caaatacttt tttcaaaaaa gccgatgtgt attctacagt aggttgatgc
     2161 cagggttcaa agagccagaa gctatgcgga ctgtcctcga atgtgtgaac ttcagcataa
     2221 atattaaatt ttttcagcag acggacgtgc tcgtcgcgcc cgttgtgaaa acgtggaata
     2281 gagctgttga tgaaacaaac cggcgctgaa aatttattga caaaataaac ggctgaggca
     2341 tctttccatg catccggttt ttcggcccgg gttccaccga gccactgcat atctgcgggc
     2401 atttttccgc ctttctcccg aaattgttca gcccgatcga tggtagtctg atccatgaat
     2461 gttgaaatac catcaatatt caccaccgcc cggattaaac gatctttatt tttggttccc
     2521 agcaaggctg caagttgtcc accggctgaa caaccctcta ctgctattct tttccggtcg
     2581 aaaccatact ctctggcatg tttataaatc cacaaaacag catcgctcag gtcatccatc
     2641 gcagccggat aaatggcttc gggtgaaagg cgatattcaa caaccaccgt ggcaaatccc
     2701 tgcctgctca ggctgacagc aagcgattgc tgcatccggg gcgatccgga gctccaccct
     2761 ccaccatgga tcatcagtac tacgggaagt tttcggggtt ttaacggcct gtaaatattt
     2821 agcttcaatt c
//
LOCUS       DAOPZK010000203         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_49798_length_2817_cov_21.815351, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000203 DAOPZK010000000
VERSION     DAOPZK010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49798_length_2817_cov_21.815351"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(826..2775)
                     /gene="thrS"
                     /locus_tag="PKH68_08925"
     CDS             complement(826..2775)
                     /gene="thrS"
                     /locus_tag="PKH68_08925"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNX13097.1"
                     /translation="MINITFPDGSVRQFAEGTTGLQIAESISSRLAQEVLSIGVNGET
                     WDLSRPIYSDASIKLYKWDDEEGKHAYWHSSAHLMAEALQELYPGIKFGIGPAIENGF
                     YYDVDPGEAVIKEADLQAIENKMTELAARKEAIVRTDISKADALKLFGDKGDEYKTEL
                     ISDLADGTITLYSQGNFTDLCRGPHLAHTGEIKAIKMLSVAGAYWRGDEKRKMMTRIY
                     GISFPKRKMLDEYLVLLEEAKKRDHRKIGKELELFMFSETVGKGLPMWLPKGTALRLR
                     LEDFLKRIQRKYEYQQVMTPHIGNKLLYVTSGHYAKYGKDSFQPIHTPEEGEEYLLKP
                     MNCPHHCEIYKHKPRSYKDLPVRLAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCR
                     PDQVKTEFLKVMDIISIIFKALEFTKVEVQISLRDPNNKEKYIGSDENWEKAEQSIVE
                     ACQEKGLDAKVELGEAAFYGPKLDFMVRDAIGRKWQLGTIQVDYNLPERFELEYTGED
                     NQKHRPVMIHRAPFGSMERFVAVLIEHTAGKFPLWLTPEQAVILPISEKFNDYAREIA
                     KSLDAQDIRVTVDDRNEKIGRKIRDNELKRIPYMLIVGEKEAENGEVSVRKQGDGDKG
                     TMKVDAFAEMVNNEVNATMKEAMNF"
BASE COUNT          686 a          619 c          644 g          868 t
ORIGIN      
        1 actttggtta attgcccaaa caaggaagga gtagtggatt gttactttgg ttaatcgccc
       61 aaacaatgaa ggagtagtgg attgttactt tggttaattg cccaaacaag gaaggagtag
      121 tggattgtta ctttggttaa ttgcccaaac caggaaggag tagtggaaag tgttttggga
      181 caattgtcca aagttggtat tagggaagcc atttaaacaa ccgccagatg tcgaatagtc
      241 ttcacaagta ggcagacagc ctttaactac ttttcgttaa tatcgaatga cacacaacaa
      301 tagggaatct cctctggaat ttttgggtct gctgaaaaat tcaactgaaa aatctttcgg
      361 tacgctatac cttaaaattc gaagaaaatt ccttttctat taacctgctg caggtaggtg
      421 tgtggcttat atttagaagt agcccaccga aacaggatgc aaggaatatt gtattttttt
      481 attcaaaaaa ctttcattta aaatcctgtc tgccgacaga taagaacttc aacataattt
      541 cgtgtaatat aatccatatg tgtttgaaaa gaaattggaa aaactacatt gctgttcaat
      601 cggcattgag cataaaaaac atacatagac aattgggttt cctaaccgct ttgtatttct
      661 ttcaagcggt taggaaaccg cttctcctcc gtatcgtaca aaaaaacctg acaagtttcc
      721 aatacttgtc aggtttaatt tagataatat tcttatactc acttcttaaa gtctccctta
      781 tggggagatt tagaggggct tcaacttcgg gaaggtttag atgggctaaa aattcatcgc
      841 ctccttcatc gtagcattga cttcattgtt caccatttcg gcaaatgcat cgactttcat
      901 cgtgccttta tctccatcac cttgcttgcg caccgatact tcaccgtttt cggcttcttt
      961 ttcgcctaca atcagcatat aagggatgcg tttcagttcg ttgtcgcgga ttttacgtcc
     1021 tattttttcg ttgcggtcgt ccacggtgac gcggatatcc tgcgcatcga gcgattttgc
     1081 tatttcccgg gcataatcat tgaatttttc actgatgggc aatatcactg cctgttcagg
     1141 agtgagccac aacgggaatt ttccggcggt gtgttcaatc agtacggcta caaatcgttc
     1201 catagaaccg aagggtgcac ggtgtatcat cactggtcga tgtttctgat tatcttcacc
     1261 cgtgtattcc agttcgaagc gttcgggcag gttgtaatcc acctgaatgg tacccagctg
     1321 ccatttacga ccgatggcat cacgaaccat aaagtcgagt ttggggccgt agaaagcggc
     1381 ttcacccagt tcaaccttgg catccagtcc tttttcctga caggcttcca caatggattg
     1441 ttctgctttt tcccagtttt catcactgcc gatgtatttt tccttgttgt tcggatctct
     1501 tagtgaaatc tgcacctcaa ctttggtaaa ttccagcgct ttgaaaatga tactgataat
     1561 atccatcacc ttgaggaatt cagtcttcac ctgatcggga cggcagaaga tgtgggcatc
     1621 gtcctgggta aaacttctta cgcgtgtcag accgtgaagt tcaccgctct gttcgtagcg
     1681 atacacagta ccaaattccg ccagacgtac cggcagatcc ttgtaggagc ggggtttgtg
     1741 tttgtatatt tcgcagtggt gcgggcagtt catgggtttg agcagatatt cttcaccttc
     1801 ttcgggggtg tgaatgggtt ggaacgaatc cttaccgtat tttgcgtaat gtcccgatgt
     1861 aacatacaac agcttgttgc cgatatgcgg ggtcattacc tgctgatatt cgtatttgcg
     1921 ttgaatgcgt ttcaggaaat cttccagacg aagacgcaaa gcggttcctt tcggcagcca
     1981 catgggtaat ccttttccta ctgtttccga aaacatgaaa agttccagtt ccttgccaat
     2041 tttgcggtgg tcgcgttttt tggcttcttc gagcagcacg aggtattcgt ccagcatttt
     2101 gcgtttcggg aaactgatac cgtagatacg ggtcatcatt ttgcgtttct catcgccacg
     2161 ccagtaggct ccggctacgc tcagcatttt aatggctttg atttcgccgg tatgggcgag
     2221 gtgcggacca cggcaaaggt cagtgaaatt gccctgtgag tagagggtga tggttccgtc
     2281 ggccaggtcg ctgatcagtt ctgttttata ctcatcgcct ttatcaccga agagtttcag
     2341 cgcgtcggct ttgctgatgt cagtacgcac gatggcttcc ttgcgtgcgg caagttcagt
     2401 catcttgttt tcgatggctt gcaggtcggc ttccttaatc acggcttcac cgggatcaac
     2461 gtcgtaataa aatccgtttt cgatagcggg acctatacca aatttaatac ccggataaag
     2521 ttcctgcaat gcttccgcca tcaggtgggc agaactgtgc cagtaggcat gtttgccttc
     2581 ttcgtcgtcc catttgtaca acttaatgga agcgtcggag tagatagggc gggagagatc
     2641 ccaggtttca ccgtttacgc cgatagataa tacttcttgt gccagccgtg agcttatact
     2701 ctcggcaatt tgcagtccgg ttgtgccttc ggcaaactga cgaaccgaac cgtcggggaa
     2761 tgtaatgtta atcatacttt ttttgaacct tacaaatggt ttttaaatgg tttttaa
//
LOCUS       DAOPZK010000204         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_50729_length_2784_cov_21.475632, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000204 DAOPZK010000000
VERSION     DAOPZK010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50729_length_2784_cov_21.475632"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PKH68_08930"
     CDS             complement(<1..1085)
                     /locus_tag="PKH68_08930"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNX13098.1"
                     /translation="MKKLICNANIVNEGQIFKGSVLIGHDRIEHIYRDGDEIVLKNPV
                     EKIEAEGMWLLPGVIDDQVHFREPGMTAKGDIFTESRAAVAGGVTSYMEMPNTKPQTT
                     TLELLEEKYALAATRSMANYSFYLGATNQNIRELVKVNPADVCGVKVFMGSSTGNMLV
                     DDLDTLNRIFSEVPTLIATHCEDEPTIQKNLSYYKNIYGDNIGCEFHPFIRSEEACYK
                     SSSFAVEMAVKYGTRLHVLHLSTAREMSLFSNSSPVRDKKITAEACVHHLWFSSDDYA
                     RYGSRIKWNPAIKTENDRLALIEAVNSGKIDVVATDHAPHLIEEKSNNCYKSYSGGPL
                     VQHSLVAMLQLAKQGYFTVEKVVEKMC"
     gene            complement(1139..1879)
                     /locus_tag="PKH68_08935"
     CDS             complement(1139..1879)
                     /locus_tag="PKH68_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNX13099.1"
                     /translation="MPSKLVIIPTYNEKENIENIIRAVFALPEEFHLLVIDDGSPDGT
                     AAIVKKLQNEFPERLFIVERKGKLGLGTAYIAGFKWGLEHQYDFIFEMDADFSHNPQD
                     LPRLYNACANEGADVAIGSRYVSGVNVVNWPIGRVLMSYFASKYVKFITGLKIYDTTA
                     GFKCYRREVLETIELDKIRFKGYAFQIEMKFTAYKCGFTLREVPIIFINRQLGTSKMS
                     GGIFGEAFLGVIQLKLTSFTRKFPQKKK"
     gene            complement(1929..2735)
                     /locus_tag="PKH68_08940"
     CDS             complement(1929..2735)
                     /locus_tag="PKH68_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNX13100.1"
                     /translation="MDRLRFRSLASGSSGNCYFLGNASYGILIDAGVGVRAIRLFLRN
                     MGLDFSNIWGLFITHDHADHIKAAGVIGEKYHVPVYTTRQIHDGINRSYCVTQKLSTS
                     QRFININEVTEICDFRITAFPVSHDASENVGYHIEYRGKNFTFATDLGHVGPEATNYL
                     IKSDYMVLEANYDDDMLENGPYPLYLQARIKSDTGHLSNLQTARFLAENYTERWKYIF
                     LCHLSKENNLPELAYNTIADHLRNEEIYFEENLQLIALDRMNPSAMYLFE"
BASE COUNT          758 a          616 c          585 g          825 t
ORIGIN      
        1 tgacacattt tctcaaccac tttttccacc gtaaaataac cctgctttgc aagctgaagc
       61 atggctacga gtgaatgttg taccaatgga cctcccgaat aggatttgta acagttatta
      121 cttttttctt cgatcagatg cggtgcatga tccgtagcaa ccacgtctat ttttccactg
      181 ttgactgctt caatcagtgc aagccggtca ttctccgttt ttatagccgg gttccattta
      241 atgcggctac cgtagcgggc gtaatcgtcg gacgaaaacc acaaatgatg tacgcaggct
      301 tcggcagtta tttttttatc tcttacggga gagctgttgc tgaataatga catttcgcgg
      361 gcagtggaca aatgcagcac atgcagtctg gtgccgtatt tcacagccat ttccacagca
      421 aatgaggatg atttgtaaca agcttcttca gatcggatga atggatgaaa ttcacatccg
      481 atattgtcac cgtagatatt tttataataa ctgagatttt tctgaatagt gggttcgtcc
      541 tcacagtgtg tagcaatgag ggttggcact tcggaaaaaa tccggtttaa tgtgtccaga
      601 tcgtccacca gcatattgcc tgtagaagaa cccataaata ctttcacccc acacacatca
      661 gcaggattaa ctttcaccag ctcgcgtata ttctgattgg tggcgccgag ataaaaagag
      721 taattagcca tcgacctggt agctgccagg gcgtattttt cttccagaag ttcaagtgtg
      781 gtagtttggg gtttggtgtt tggcatttcc atatacgagg tcacaccacc agccactgca
      841 gccctgcttt cggtgaatat atcgccctta gcggtcatac cgggctccct gaaatgcacc
      901 tgatcatcaa tcacaccagg gaggagccac attccttctg cttcaatttt ctcaaccgga
      961 ttttttagta ctatttcatc gccatcccga taaatatgtt ctatccggtc atgaccaatc
     1021 aaaactgaac ctttgaaaat ctgtccttca ttgacaatat tcgcattgca aattagtttt
     1081 ttcataatgc tttcagttta aagggttagt taagcgaaag tatgtaaaat atttgttatt
     1141 atttcttttt ctgaggaaat tttcgggtaa aactggttaa ttttaattga ataactccta
     1201 aaaaggcttc tccgaaaatg ccgccactca ttttggatgt tcccagctga cgattgatga
     1261 agataatggg aacttctctg agtgtaaatc cgcacttgta ggcggtaaat ttcatctcta
     1321 tctgaaacgc atagcccttg aaccggattt tgtccagttc gatggtttcg agcacttcgc
     1381 ggcgatagca tttgaaaccg gcagtagtat catatatttt caatccggta atgaatttca
     1441 catacttgga tgcaaaatac gacatgagca cgcggccgat aggccagttg accacattta
     1501 ccccgctcac atacctcgac ccgatggcca catcggcacc ttcattggca caggcattat
     1561 aaagtcgcgg caaatcttga gggttgtgcg agaagtcggc atccatttcg aaaataaaat
     1621 cgtactgatg ctccagtccc cacttgaaac cggcaatgta ggccgtacca agtccaagct
     1681 tcccttttcg ctctacaata aacaggcgtt cggggaattc attctgcagt tttttgacaa
     1741 tggctgccgt accatcgggc gatccgtcgt cgattacaag caggtgaaac tcttcgggta
     1801 gtgcaaatac ggcacgaatg atgttttcga tattttcctt ctcgttgtaa gtaggaataa
     1861 tgacaagttt tgagggcata aagtgtaaaa ttttattttt gttgcaagga taatgatata
     1921 actcagtatt attcaaacaa atacatagcc gaagggttca ttctgtcgag tgctatgagt
     1981 tgcagatttt cttcaaaata aatctcttca tttctcaggt gatccgcaat ggtattgtaa
     2041 gccagctctg gtaaattatt ttccttgctc aggtggcaga ggaaaatata cttccatctt
     2101 tctgtataat tttcggccag gaaacgggct gtttgcagat tgctcagatg cccggtatcc
     2161 gacttgatac gagcctgaag atacagcgga tagggtccgt tttcgagcat atcatcatcg
     2221 taattggctt ccagcaccat ataatccgat tttatcaggt aattggttgc ttcgggaccc
     2281 acatgtccca gatcagtggc aaatgtaaag ttttttcccc tatattcgat gtgatatcct
     2341 acattttcgg aagcatcgtg cgacacaggg aatgcagtga tacgaaaatc gcagatttca
     2401 gttacctcgt tgatattgat aaaacgttgc gaggtggata gtttctgggt aacacagtaa
     2461 ctccggttga tgccatcgtg tatttgccgg gtggtataaa ccggtacatg atatttttcg
     2521 ccgataacgc ctgccgcttt gatgtggtcg gcgtggtcgt gagttatgaa caatccccag
     2581 atattggaaa aatcaagacc catgttgcgt aaaaaaagcc ggatagcccg tactcccact
     2641 ccggcatcaa tgagaatgcc ataggaagca ttccccagaa aataacaatt accgctactg
     2701 ccgctcgcca gacttctaaa acgcaatcta tccatttttt cacatacaaa ttagctgcaa
     2761 aattaacaca aaaaacctaa tata
//
LOCUS       DAOPZK010000205         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_51008_length_2773_cov_23.486387, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000205 DAOPZK010000000
VERSION     DAOPZK010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51008_length_2773_cov_23.486387"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKH68_08945"
     CDS             complement(<1..212)
                     /locus_tag="PKH68_08945"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNX13101.1"
                     /translation="MEVIKFPPRDQWASIQTRPVFDYTVLFDRVQKILDDVRQHGDKA
                     LINYTKQFDNVNLDALEVTKEEIAEAA"
     gene            complement(247..966)
                     /locus_tag="PKH68_08950"
     CDS             complement(247..966)
                     /locus_tag="PKH68_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNX13102.1"
                     /translation="MIQEAISSYLNKHNFPGFQLKAVFFDMDGVLFDSMPQHSIAWVK
                     AMNDVGLEFTQYDAYMNEGQTGTATINGVFVLKNGREATEEEKQEIYKRKAAYFEALN
                     SPEKMPFVYDLLKKIKEEGYQLFVVTGSAQPSLLHSLQDHFPGIFEKENIISAFDVTH
                     GKPHPEPYLKALKKAGVNPWEAVVIENAPLGVQASTAARIFTIGVNTGPLDDEVLMNS
                     GAGIVLNSMQDLYEQWDSFGF"
     gene            1166..1238
                     /locus_tag="PKH68_08955"
     tRNA            1166..1238
                     /locus_tag="PKH68_08955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1199..1201,aa:Pro,seq:cgg)
     gene            1815..2336
                     /locus_tag="PKH68_08960"
     CDS             1815..2336
                     /locus_tag="PKH68_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13103.1"
                     /translation="MKLRLILPLLLMIAAFTACNLDNTANTTPTIAIYAPRIGDSIVR
                     MSYTDDMSALKVDSLHVGDTVSMMVVGDARANNLTEFNVVPSDTTAVKILIPDSIEYY
                     VASGSIPAEGKFLMKPKTVLFYYPLNFIPRKPVDKMKFTFKVTSDAVSVPNQSAFEFS
                     FPIKPKRIKPTAQ"
     gene            complement(2409..>2773)
                     /locus_tag="PKH68_08965"
     CDS             complement(2409..>2773)
                     /locus_tag="PKH68_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNX13104.1"
                     /translation="YQELLRNAKNIKEILDIEEKIRVLEEEIESTTGRLKYLNDQVNY
                     STLELNISQKKAFKYTPPFRQNFFEKLKQSLSRGWYGFVDLFFFLLSNWAVLILLAVL
                     IWLWIKYRKRRKSRKQKE"
BASE COUNT          803 a          582 c          541 g          847 t
ORIGIN      
        1 gctgcttcgg caatttcttc tttggtaact tccagtgcat cgaggtttac attatcaaac
       61 tgctttgtat aatttatcaa tgccttgtcg ccatgctgac gcacatcatc cagaattttc
      121 tgaacacgat caaaaagcac cgtataatcg aataccggac gtgtttgtat tgatgcccac
      181 tgatcacggg gcggaaattt tattacttcc atataattaa atgagaggaa attaatattc
      241 taaatttcaa aagccgaaac tatcccattg ttcgtacaaa tcctgcatac tattcagcac
      301 aatgcctgct ccgctattca taagaacctc atcgtctaac ggtcctgtat ttactccgat
      361 ggtgaaaata cgggcagcag tggacgcctg tacacccagt ggtgcattct ctatcactac
      421 agcttcccac ggatttaccc cggctttctt cagtgccttg aggtaaggtt cgggatgagg
      481 ttttccatgt gttacatcaa atgccgatat tatattttct ttctcaaaga tacccggaaa
      541 atgatcctgc aggctgtgga gcagcgatgg ctgcgctgag ccggtcacca caaacagctg
      601 ataaccttct tctttaattt ttttcagcaa atcatacaca aagggcatct tttcgggaga
      661 attcaatgct tcgaaataag ctgctttacg tttgtagatt tcctgctttt cttcttcggt
      721 agcttcccgg ccgtttttga gtacaaatac tccattgata gtggccgtgc cggtctgacc
      781 ttcgttcata taggcatcat actgtgtgaa ctcaagtcct acatcattca ttgctttcac
      841 ccaggcgatc gagtgctggg gcatggagtc aaacaacacg ccgtccatat cgaaaaatac
      901 cgctttgagc tgaaatccgg gaaaattatg tttgttcagg tagctgctga tggcttcttg
      961 tatcatgcta aaaaaaattt tgacaaagat acaaaaaaat gttcttttca gtgattaccg
     1021 actaaaataa cagtaattca ccccggttgg taactgaatg taaatcagaa agtaaataca
     1081 atacagactg aagagtgaat aataaaagga aaaatttgtt tcttttttcg taaaaatgca
     1141 ttaaatttgc atccgaaatc aaaagcggga tgtggcgcag ttggtagcgc gctacgttcg
     1201 ggacgtagag gtcgcaggtt cgaatcttgt catcccgact ttacatcaat aagggtgctc
     1261 cgatagtgga cacccttatc tgttttcaaa gaaacacgga ttgatttgaa ccggttgtct
     1321 gccattttga caggtaaaac gaacgatgat ttttgcttac agtccttcag gagattgtgt
     1381 cacaacggac caggggatgt aaacattggg taagaaagaa atttgaaatt aattaatgga
     1441 attacctgtt tgaaaatcat tgttgtccgg taaaaatatt tagatccatc gcttcgctct
     1501 gcctgcctgc cgcagacagg gatgacaggg aattatggcg ttttaggtaa ggagggggtt
     1561 ggttggcggc gaagccgcca accaaccccc tccttaaaga taaaatactg actgtcattt
     1621 cgaacgtagt gagaattgaa aatcggcgta gccaaatcta atatatatca agaattttga
     1681 attttaccgg acaatagtaa ttgaaaatta aaaataaatt atgacagaag ccaatctggc
     1741 atggaatttg tacaataaat gagtgtttat cacgtttttt aacccaaata taaaacatac
     1801 caaaatatat acctatgaaa ctccggttga tattacctct gctgctgatg atagcagctt
     1861 tcacagcctg taatctggat aacacggcta acacaactcc tactattgct atttatgctc
     1921 cccgcatagg tgatagtatc gtgcggatgt catataccga tgatatgtct gcattaaagg
     1981 tagactcact gcacgttggt gatactgtct ccatgatggt agtcggcgat gcccgcgcca
     2041 ataatctgac tgaatttaat gttgtgccga gtgacaccac cgctgtgaaa attctaattc
     2101 ccgattcaat tgaatattat gtggcttcgg gctccatacc tgctgaagga aaattcctga
     2161 tgaaacccaa aacggtttta ttttactatc cgctgaactt tatcccccga aaaccggtgg
     2221 ataaaatgaa atttacattt aaagtaacct ccgatgcagt ttctgtaccc aaccaatctg
     2281 ccttcgagtt ctcgtttccc atcaaaccga aacgaataaa acctaccgct caataaaata
     2341 taaaggctgt ctcaacaaat aaatgagaca gccttttttt atggagaatt tttcagcctt
     2401 tcccctgctt attctttttg ttttctgctc tttcttcttt tccggtattt aatccataac
     2461 cagataagca cggccagtaa aattaaaact gcccagttgg acaacaggaa gaaaaagaga
     2521 tccacaaaac cataccatcc tcttgaaagt gattgtttca gtttttcgaa aaagttttgt
     2581 ctgaacggag gagtgtactt gaatgctttc ttctgcgaaa tgtttaattc gagtgtgctg
     2641 taattgacct gatcattcag gtatttcagt ctgccggtgg tactttctat ttcttcttca
     2701 agtacacgta tcttttcctc aatatccagg atttctttga tgttttttgc attacgtagt
     2761 aattcctgat agc
//
LOCUS       DAOPZK010000206         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_51268_length_2764_cov_20.212994, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000206 DAOPZK010000000
VERSION     DAOPZK010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51268_length_2764_cov_20.212994"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..633)
                     /locus_tag="PKH68_08970"
     CDS             complement(214..633)
                     /locus_tag="PKH68_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNX13105.1"
                     /translation="MKQSIKASWKGDLAFETELNGHKLVMDAPEEGGGHDLGPRPKIL
                     MLAALAGCTGMDVVSILGKMRVPVDKCDVHVEANMTDEHPKQYDKMHVIYEFTGKNLP
                     PDKLEKAVRMSEETYCGVGALYRKAIEVTSEIKIIEG"
     gene            1054..1965
                     /locus_tag="PKH68_08975"
     CDS             1054..1965
                     /locus_tag="PKH68_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNX13106.1"
                     /translation="MQPISSFKDLTSHLAEANCRKKLAVANAVDSHTLDAVLMAVEAG
                     IVEAYLVGDVAAIESPELFEKPSILPFIHIIDKPEVVEATLEAVRMVKDGEADILMKG
                     LVNTDVILRAILDKEKGLLPPGNVLTYNAALEIPGYHKLLFFSDPAVIPYPTLEQRKA
                     MIKYAINTCKKFGFQHPKVALIHATEKANPKIMFMQDYLDIMQMWRMGEFGDVIMDGP
                     LDIFLALDTERGSIKNVPSPVLGDADVLIFPDFAAANVFYKGVALFANAQMGGILQGT
                     SKPCVLTSRSESSLSKFYSIAVACILA"
     gene            2304..2615
                     /locus_tag="PKH68_08980"
     CDS             2304..2615
                     /locus_tag="PKH68_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNX13107.1"
                     /translation="MDNNENQNQLNIELSAEVAEGTYSNLAIISHSSSEFVIDFIRMM
                     PGTPKANVKSRIILTPEHAKRLLFALQDNINKYEQQNGKIKVPDNNQLPPIPMGFGGE
                     A"
BASE COUNT          763 a          591 c          577 g          833 t
ORIGIN      
        1 ccggagatta cagcataccg caatcaataa cggacttttg aaaaaaatag tacatccttt
       61 aatgttaaaa taaacattcc aattcagtca taaagactga tgatatcgat aaatagtcat
      121 tttttttgag tttagctgat taaataaaaa aacttccgga agttttatta ccgaaagttt
      181 ttttgtttta atgttgagtt tcaggttttc ttatcaaccc tcaatgattt ttatttcgga
      241 ggtgacttct atcgccttgc gatacagcgc acccactccg caataggttt cttccgacat
      301 ccgcacagct ttttctagtt tatccggagg aagatttttg ccggtaaatt cgtaaataac
      361 atgcatttta tcgtactgtt tgggatgctc atccgtcatg ttggcttcca cgtgtacatc
      421 gcacttatct acgggtacgc gcattttccc aagaatggaa accacatcca ttcctgtgca
      481 accggccagt gctgccagca tcagtatttt tggtcgtggc cccaaatcat gtccgccgcc
      541 ttcttcggga gcatccatta ccagtttgtg accattgagt tctgtttcga aagccaaatc
      601 tcccttccag cttgctttaa tcgattgttt catatgttta tcatttttta aatctgcccc
      661 ctaatccgtc agctgacgga ggaatattga gcgtctatcg tttgttatgc gttatttaat
      721 cataccattg tgtgtccaaa atttttagtt ggacattggt gtttcatatt tcttttgatt
      781 ggttttttta tgtttatact ccttagaaac atttccggtc aaaaaaagtt ttttgatgga
      841 aaacaaaaaa agttcataaa atattttttg ccatttttcg gcaatctatt catggaaacg
      901 ggggaattta ttttaaaatt atcaggaaac attttatgga ataactgttt gtaaattagg
      961 ctctgaaaaa tgaccggcac cagcccccgc tataacttgc aggctgaaaa taaatttata
     1021 tatttgcatg gatttacacc gaaccgtcac cctatgcaac ccatatcatc gttcaaagat
     1081 ctgacttcac atctggcaga agccaattgc cgcaaaaagc tggctgtagc caatgccgtg
     1141 gattcgcata cgctggatgc tgtacttatg gctgtagaag ccggaatagt ggaagcctat
     1201 ctggtgggcg acgtggctgc catcgagtcg cctgaattgt tcgagaaacc atccattctc
     1261 ccttttattc acattatcga caaacctgaa gtggtggaag ccaccctcga agcagtgcgt
     1321 atggtgaagg acggtgaagc cgatattctg atgaaagggc tggtgaacac cgatgtgata
     1381 cttcgcgcca ttctggataa agaaaaagga ttgctccctc cgggaaatgt gctgacttac
     1441 aacgcagcgc ttgaaattcc ggggtatcat aaactgctgt ttttctccga ccctgccgta
     1501 attccctacc ccacgctgga gcaacgaaag gctatgataa aatacgccat taatacctgc
     1561 aaaaagttcg gatttcaaca tccgaaagtg gcattgatac acgctaccga aaaggcaaat
     1621 cccaaaatca tgtttatgca ggattatctg gacatcatgc agatgtggcg tatgggtgaa
     1681 tttggcgatg tgatcatgga cggaccgctg gatatatttc tggcactcga cactgagcgc
     1741 ggaagtataa aaaatgtgcc ttcacccgtg ctgggagatg cggatgtgct tatctttccc
     1801 gattttgctg ctgccaacgt gttttataaa ggtgtagcac tttttgcaaa tgctcagatg
     1861 ggcggtattc tccagggcac ttcaaaaccg tgtgtgctga cttcgcgcag tgaaagttca
     1921 ctgtcaaaat tttacagtat tgcagtggcg tgcattctgg cgtaaaagcc caatataacg
     1981 acatattcgg gtgcggcggt ttttaaccgc cgttttcatt ttaatataag cggttacaaa
     2041 ccgcttcacc cactgaatac gtacgaactt ctcgggtatg gcggttttca accgccgaaa
     2101 aaaccagtaa aaaaccgctt catccaactg atattttttt tgtaacttta ccccctgatt
     2161 gggtagtcta cttttcgcgt tacagtttcg ggatgaaacg tccattctgt ttcggaaagt
     2221 ccttgcttca acttgctgag ttacagaaat aacctgacac agcctgcatt aatgtaaaaa
     2281 accaccatta acaacatact attatggaca acaacgaaaa ccaaaatcag ctgaacattg
     2341 aattatcggc tgaagtagct gaaggaactt actcaaacct ggcaatcatt tcacattcct
     2401 catcggaatt tgtaatcgac tttattcgca tgatgcccgg aactcccaaa gccaacgtta
     2461 aatcacggat cattcttact cccgaacatg ccaaacggtt gctatttgca ttgcaggata
     2521 atatcaacaa gtatgagcag caaaacggca agattaaagt tcctgacaat aatcagttac
     2581 cgcctattcc aatgggattt ggaggggaag cgtagacgtt caacgtttga cgtttgaggt
     2641 ttgaggtttg acgttcaacg tttgaggttt gaggtttgag gtttgaggtt caacgatttt
     2701 tcaattcgct gtagatcatt tcgtagagtg gaacggggat gttgtgcttg tgagccaggc
     2761 gaac
//
LOCUS       DAOPZK010000207         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_52234_length_2729_cov_26.375841, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000207 DAOPZK010000000
VERSION     DAOPZK010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52234_length_2729_cov_26.375841"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..>2729
                     /locus_tag="PKH68_08985"
     CDS             291..>2729
                     /locus_tag="PKH68_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNX13108.1"
                     /translation="MKKTVIFLFLIFTLPVLAVGQVSFGKPEKINDGWRFILQDVKDG
                     QERNINDSRWEKIDLPHDWSVKGLLSPTLASCTGYLPGGIGWYRKTLNIPKERANEKV
                     FLYFEGVYNRSEVFVNGHSIGSRPNGYISFLYDATPYIVFGEDNVIAVRVDHSRSADS
                     RWYTGSGIYRNVWAIYSNPVHVAQWGVYAYPSRNKNQHALNVEIEIENETSAGQTLTV
                     VNELLDKNKKVVARSSNKIQTPANQSFKLTGNLKVKNPALWSLENPYLYELKTTLLQN
                     GKVIDASTLNTGFRYFTFDPDKGFALNDTRMKVKGVCLHHDAGVLGSAVYRDVWKRRL
                     QNLKEIGVNAIRTSHNPQASAIYELCDELGLLVIDEAYDEWEFPKRKWIEGWNKGTPG
                     FQGSFDYFAEWAEKDLGDLVKRDRNHLSVFAWSIGNEVDYPNDPYSHPILDGGKEGFT
                     QPIFGGYKKEAPDAMRLGAIAKKLVAVVKQYDKSRPTTAGLAGVAMSNETEYPGALDI
                     AGYNYTESMYDRDHERYPQRVIFGSENRHELSAWKAARDKDFVFGQFLWTGIDYLGES
                     GSWPSRGFYSGLLDFGGFIKPRGYFRQSLWSDKPMAYLGTYPTPTRGSRSQMKDVWSQ
                     LDAENAGNYEEKVPSMDAWPVWNYQDGQLIRVVCYTNAPKARLLLNGKEVGQTQSYNE
                     NTGIIYWDIPYEQGTLSVAGLDKNDKEISNYEIKSSKQPYALEITAADKSVSKGHVAQ
                     ITVQVVDENGIPVMLSDNEITCRIVGDGKLLGLEASNNEDMSDYNDNKQRVYHGRMLA
                     YIKPTKEEGSIKVRF"
BASE COUNT          824 a          522 c          666 g          717 t
ORIGIN      
        1 ggaatataaa tgcttgtcag tataaaaaat ggtcaaattg gtgcaaatga tgtgcatttg
       61 ccgatattat tacatttgat tgattgtttg aatgattata atgaataatt ttacagagtt
      121 tgaaaagctt ttttcttttt tcagataatg atccggataa tacaaaatcg ctttcattag
      181 ccatcggaag aatgcggttc gatgctcatc ctttgaaaaa attcatgttc aataacccaa
      241 actaatttcc tgtgacaatc aggagtgatg gtttatttaa aaatattcgt atgaaaaaga
      301 ctgtaatttt cctgttctta attttcactc tgccggttct tgctgttggg caggtttcat
      361 tcggtaaacc ggagaaaata aatgatggct ggcgtttcat ccttcaggat gtaaaggatg
      421 gtcaggaaag gaatattaat gattcgcgct gggagaaaat tgacttaccg cacgactgga
      481 gtgtgaaagg attgctgagc cctacgctgg caagctgcac gggttatctg cccggaggga
      541 taggctggta tcgtaaaact ttaaatattc ccaaagaaag agcaaacgaa aaagtctttc
      601 tttattttga aggagtttac aaccgcagcg aggtatttgt caatggtcat tctatcggct
      661 cacgacccaa tggatatatt tcgttccttt atgacgccac accctatatt gtttttggag
      721 aagataatgt catagccgta cgggttgatc acagccgctc tgctgattca cgctggtaca
      781 ccggctccgg aatttacaga aatgtttggg ccatttattc aaatcccgta catgttgctc
      841 aatggggtgt atatgcctat ccgtcccgaa acaaaaatca acacgcactg aacgttgaaa
      901 ttgagattga aaacgaaact tctgccggac aaactttgac cgtagtaaat gaacttttgg
      961 ataaaaacaa gaaagttgtt gccagaagca gtaataaaat tcagacccct gccaatcaat
     1021 cctttaaact tactggaaat ttgaaagtaa aaaatccggc gttgtggagt ttggaaaatc
     1081 cttatttgta tgaactgaag acaacattgc tgcaaaacgg aaaagtgatt gacgcatcca
     1141 ctctcaatac cgggttcaga tattttactt tcgatccgga taaaggtttt gcgctgaatg
     1201 atacccggat gaaggtgaag ggtgtgtgcc tgcatcacga tgccggtgta ttgggctcgg
     1261 ctgtttaccg ggatgtctgg aaaagaagat tgcagaatct gaaagaaata ggagtaaatg
     1321 ccatccggac aagtcacaat cctcaggcat ctgctattta tgaactttgt gacgaattgg
     1381 gactgcttgt gatcgacgaa gcttacgacg aatgggaatt ccccaaaaga aaatggattg
     1441 aaggttggaa taagggaact cccggttttc agggttcatt tgattacttc gcagaatggg
     1501 ccgaaaaaga cctgggagat ctggtaaaaa gagaccgcaa tcacctttcg gtttttgcct
     1561 ggagtattgg caatgaggtg gattatccga atgatccgta ttctcaccct attctggatg
     1621 gcggcaaaga aggatttacc caaccgattt tcgggggata taaaaaagag gctcccgatg
     1681 caatgagatt aggcgcaata gccaaaaagt tggttgcggt ggtgaaacaa tacgacaaat
     1741 cacgccctac aaccgccgga ctggcaggtg tagccatgtc caatgaaacc gaataccccg
     1801 gagcgctgga tattgccggc tacaactaca cggaaagcat gtacgaccgc gatcatgaaa
     1861 gatatcctca acgggtaata tttggaagtg aaaacagaca cgaactgagt gcctggaaag
     1921 cggcaagaga taaggatttc gttttcgggc aatttctctg gacaggtatt gattacctgg
     1981 gtgaatcggg cagctggcct tcgagagggt tttattccgg attgctggat tttggaggat
     2041 ttatcaaacc gcgcgggtat ttccgccagt cactgtggtc tgataaacca atggcatacc
     2101 tgggtactta tcctacaccc accagaggaa gccgctcgca gatgaaagac gtctggtcgc
     2161 agcttgatgc tgaaaatgcc ggcaattacg aggaaaaagt tccctcgatg gatgcctggc
     2221 ccgtttggaa ctaccaggac ggtcagctca tccgcgtggt ttgttacacc aatgctccga
     2281 aagcccggtt gctgctgaat ggaaaagagg ttggtcagac ccagtcttat aatgaaaata
     2341 ccggaataat ctattgggat attccttacg agcaaggaac gctgtcggtg gcaggattgg
     2401 ataaaaatga taaagaaatc agtaattacg aaataaaatc atcgaaacaa ccttatgcgc
     2461 tggaaataac ggctgccgac aaatctgttt ctaagggtca tgtggcacaa ataacggttc
     2521 aggtggtgga cgaaaacggt attccggtga tgttgtcgga taatgaaatc acctgcagga
     2581 ttgtgggcga cggaaaactt ctggggctcg aagccagtaa caatgaagat atgagcgatt
     2641 acaacgacaa caaacagcgg gtgtatcacg gccgtatgtt ggcttatatt aagccaacaa
     2701 aagaggaagg aagtataaaa gtgagattt
//
LOCUS       DAOPZK010000208         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_52363_length_2725_cov_26.059925, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000208 DAOPZK010000000
VERSION     DAOPZK010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52363_length_2725_cov_26.059925"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2725
                     /locus_tag="PKH68_08990"
     CDS             <1..>2725
                     /locus_tag="PKH68_08990"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HNX13109.1"
                     /translation="LYGVLEVTGAENKLISTTLTTTNLTAGLTEQINSVKALMMRLLP
                     LRAAEFDFAAIDKKDDVLDVFELSSVGSKILVKGSSAPAMAAGVRYYLNNYCNASFSW
                     NGDQNVLPSPLPALTQPVRKETLFQHRYALNYCTLNYTMSFWDWERWEREIDLMATQG
                     VNLFLSGIGTEAVWQKWMEKYGYSFAEIQKFIPGPAYTAWWLMGNLEGEGGPVSQEYI
                     NGRVELQKKIIARAKELGMNVVLQGFAGFVPSTIGSKGTGVTVHDQGDWVGGYKRPVV
                     VSGPKAIEMAKTWYEESVKLFGAANFYGGDLFHEGGNIPSNLDVSKYAGDIQTEMMKV
                     NPEAVWVLQSWLGNPHELVLKGLKKDQALVIDLGNERDFGWLRHQPTAFTGVPWVFSV
                     IQNFGARMGIYGRIPAIADRYQEMRNNARRGNNWGIGIAPEAIIFNQASYDIMWDMVW
                     ESARIDPQNFITRFAGYRYGKNNANAENAWKILLNTALNCPGGSTQDGASESVIGARP
                     RLDVGNVSSWSTTKLYYDPKAIIPAWTLMVNSAQELKDQTTFRFDLVDLTRQNLSNYA
                     QILQKEWADAFRAKNKALFTEKSKLFLELILDQDSLLRTRQECLTGPWIADARKMGTT
                     ESEKNLFERNARALITTWKTTANTSLNDYSFREWAGLMKDYYYKRWKLFIDDLAGQLD
                     GKAAINYDFYNVVEKLFQIRYSVSYPISPQGDEIAVSKYLYEKYMPLFNGTQKRIYTI
                     SENQPKGTEVGTLNITGANTFELQGQSVTDAFVIDASTGKITVNKAEVMLQSENPTFT
                     LRIKATNPANPSFAEYADVTINLTATSGIAENSYGAQVRLFPNPTRSSLTVQLPAEQR
                     VQLTVSNEKGTILLQRQESGTEFLLDTINIAPGVYFLNIKSDFTNNSYKFLV"
BASE COUNT          788 a          628 c          650 g          659 t
ORIGIN      
        1 ctttacggtg tgttggaagt tacgggagct gaaaataaac ttatcagtac cacactaacc
       61 actaccaatt taacagctgg cctcaccgag cagatcaaca gcgtaaaagc cctgatgatg
      121 cgtctgcttc cattgcgtgc tgcggagttt gactttgcag ccatcgacaa aaaagacgat
      181 gtccttgatg tgtttgaact ttcgtccgtt ggttccaaaa ttctggtaaa aggtagttct
      241 gctcctgcaa tggctgccgg ggtgcgttac tatctgaata attattgcaa tgcctctttc
      301 tcgtggaatg gcgaccagaa tgttctcccc tcaccacttc cggcactaac acagcctgta
      361 cgcaaagaaa cccttttcca gcaccggtat gcacttaact attgcaccct caactacacc
      421 atgtcgttct gggactggga acgttgggaa cgcgaaatag acctgatggc aacacagggc
      481 gttaacctgt tcttgtcagg tattggtacc gaagcggttt ggcaaaagtg gatggaaaaa
      541 tacggatatt catttgccga aatccagaaa ttcattcccg gacctgcata tacagcctgg
      601 tggctgatgg gtaacctcga aggtgaaggc ggcccggtaa gtcaggaata catcaacggc
      661 cgggttgaac tgcaaaagaa aatcattgcc cgtgccaaag agctgggtat gaatgttgta
      721 ctgcagggat ttgcgggatt tgtaccttct accatcggtt caaaaggtac cggtgtaact
      781 gtacacgacc agggcgattg ggtgggaggt tacaaacgcc ccgtggtggt gagcggtccc
      841 aaagccatag aaatggcaaa aacgtggtac gaggagtcgg ttaaactgtt tggtgccgcc
      901 aacttttatg ggggtgacct ctttcacgaa gggggaaata taccgtccaa tctggacgta
      961 agcaaatacg ccggagacat tcagaccgaa atgatgaaag taaatcccga agcagtttgg
     1021 gtacttcagt catggctcgg aaatccccat gaattagtcc tcaaagggtt gaaaaaagat
     1081 caggcattgg taatcgacct tggcaatgaa cgtgattttg ggtggctgcg tcatcagcca
     1141 acagctttta caggtgttcc gtgggtattc agcgttattc agaacttcgg cgcccgcatg
     1201 ggaatctacg gccgtattcc tgcaattgcc gacagatatc aggagatgcg taacaacgcc
     1261 cgcaggggaa ataactgggg aattggtatt gcacctgaag ccattatctt caatcaggca
     1321 tcatacgaca ttatgtggga catggtatgg gaaagcgccc gcattgaccc gcaaaacttc
     1381 attacccgat ttgccggtta tcgttatggt aagaataacg ccaatgctga aaatgcgtgg
     1441 aaaatccttc tgaataccgc cctcaactgt ccgggcggca gcacgcagga cggagcttcg
     1501 gagtcggtga ttggagcacg cccccgactg gatgttggaa atgtgtcgtc atggtcaaca
     1561 accaaacttt attacgatcc taaggctatt atcccggcct ggacgctgat ggtcaattcg
     1621 gctcaggaac taaaagacca gaccacattc cgttttgatt tagttgattt aacgcgccaa
     1681 aatttatcca actacgctca gatacttcaa aaagagtggg ctgatgcttt cagggccaaa
     1741 aataaagcat tattcaccga aaaaagcaaa ctcttcctcg aattgatact cgaccaggat
     1801 agcctgctgc gcacccgcca ggagtgcctc acaggaccgt ggatagctga cgcccgtaag
     1861 atgggaacta cggaaagcga aaaaaatctg ttcgagcgaa atgcccgcgc ccttatcacc
     1921 acatggaaaa ccacggcaaa cacgtcgctg aacgattatt cgtttcgcga atgggccggc
     1981 ttaatgaaag attattatta caaacgctgg aaacttttta ttgatgacct tgccggacaa
     2041 ctggacggaa aagcagctat aaactacgac ttttacaatg tggtggaaaa actcttccag
     2101 ataaggtact cagtcagtta tccgatatct ccccagggcg atgaaatagc agtttcaaaa
     2161 tatctgtatg aaaaatatat gccgctgttc aatggtacgc aaaagcgcat ttacaccatt
     2221 tctgaaaacc agccaaaagg aacggaagtt ggtacattga atataacggg agcaaatacg
     2281 tttgaacttc aaggtcagtc agtaaccgac gcttttgtga ttgatgcctc tacaggtaaa
     2341 attaccgtta ataaagcgga agtaatgtta caaagcgaaa atcccacatt cacgcttcgc
     2401 attaaagcca ccaatccggc caatccttct tttgcggaat atgcagacgt taccattaac
     2461 ctgacagcca ccagtggcat tgcagaaaac tcgtacgggg cgcaggtacg actgtttccc
     2521 aacccaacca gaagcagtct gaccgtacaa ctcccggcgg agcaacgggt tcagctgact
     2581 gtcagtaacg aaaaagggac tatacttttg caacggcagg aaagcggtac tgaatttttg
     2641 ttagatacaa taaatattgc gcccggagtt tattttctaa atattaaaag tgattttaca
     2701 aataactcct ataaatttct ggtaa
//
LOCUS       DAOPZK010000209         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_52855_length_2708_cov_20.319638, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000209 DAOPZK010000000
VERSION     DAOPZK010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52855_length_2708_cov_20.319638"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2465)
                     /locus_tag="PKH68_08995"
     CDS             complement(<1..2465)
                     /locus_tag="PKH68_08995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNX13110.1"
                     /translation="MAKKSTKLRPIPKAKTSVTYHKKPEGMTLENWQIALRKQFVMDK
                     TFGIEKIEGYHPVFGDYQVSNFETRNKYKVSIRDNRNSMNFCSCLDFKTNRLGTCKHV
                     EAVLWKIQNQPGLAGYLKKDYKISYTSVYLSYKGERKVKIRIGTDHEKEFKQLTELFF
                     DEENVLKTNAFSSFEVFLEKAYALDADFRCYNDAMEFVLNVREQNRRSEKLDKLFPQS
                     EALQGLLKTEFFPYQHQGVLFAAKAGRALIADEMGLGKTIQAIGAAELLRKEHDIASV
                     LIICPTSLKYQWQSEIERFSDSTVRVIEGLPNVRAQQYTGNEFYKIVSYHTVVNDINA
                     INVADFDMIVLDEAQRIKNWKTKIAAAVKKIKTPYCVVLTGTPLENKLEELYSIVQFI
                     DPFRLGPYYQFLNRYQIKNETGKIVGYQNLNEISQLLKGLLLRRTKKEVLSQLPERMD
                     KILFVPMTQAQKDMHEEFNAAVARLVTKWQRFGFLNEKDRQSLLINLNLMRMVCDSTY
                     IVDQTTRHDTKIQELMSILDECFEQSDEKVVVFSQWERMTRLVAQELDELGIKYEYLH
                     GGVPSQKRKALFDNFNNDPDCRVFLSTDAGSTGLNLQSASMLVNLDIPWNPAIMEQRI
                     ARIHRLGQKKNVSIINFVSKDTIEERMLSLLGFKSSLAKGILDLGEDTIFMGDDKFRK
                     FMQTVNEMTEPSEATPVHTISSDEEEEVEELSPKTDKITDAELPFDTTENFFDDDDVV
                     TDEPGEVETPTQTIPEPSHLLAQGLSFFAGLSKTLASPEKTAELISSITEKDEKTGQT
                     YLKIPLENQEVLTNVLNMVGQLFG"
     gene            complement(2500..>2708)
                     /locus_tag="PKH68_09000"
     CDS             complement(2500..>2708)
                     /locus_tag="PKH68_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX13111.1"
                     /translation="EGTSRASDKEKERFIEIAYGSLLETYCQLQIANDLNYIALEQIN
                     EIQLQIDKIANKLSALKRSYARTQ"
BASE COUNT          646 a          604 c          570 g          888 t
ORIGIN      
        1 ccaaacaact gccccaccat attcagcaca ttggtgagca cttcctgatt ttcgagtgga
       61 attttcagat aagtttgtcc tgttttttcg tctttttcgg ttatggagct tatcagttcg
      121 gccgtttttt cgggcgaagc cagtgttttg gataatccgg caaaaaagga caatccctgt
      181 gccagcagat gcgagggttc aggtatggtt tgcgtgggcg tttcaacttc gccgggttcg
      241 tccgttacca catcgtcgtc gtcaaagaag ttttcggttg tatcaaatgg tagctctgca
      301 tcggttattt tgtcagtctt tgggctcaat tcttccacct cttcctcttc gtccgaggaa
      361 attgtgtgaa ccggagttgc ttccgatggt tcagtcattt catttaccgt ctgcataaat
      421 ttgcggaact tgtcgtcacc cataaaaatg gtatcttcgc ctaaatccag aatgccctta
      481 gccagcgaac ttttgaaacc caacaggctc aacatccgtt cttcgatggt gtctttggac
      541 acaaaattaa tgatggatac gtttttcttc tgacccaggc ggtgaattcg ggcaatgcgc
      601 tgttccatta tagcggggtt ccacggtata tccaggttga ctaacatgga agccgattgc
      661 agattgagcc ccgtacttcc ggcatcggtg gagaggaaaa cgcggcagtc ggggtcgttg
      721 ttgaaattat cgaagagtgc tttgcgtttc tggcttggta ctcctccgtg caaatattca
      781 tatttaatgc ccaactcatc cagttcctga gccaccaggc gcgtcattcg ttcccactga
      841 ctgaacacca ccactttttc gtccgattgc tcaaaacatt catccagaat gctcatcaat
      901 tcctgaattt tggtatcgtg acgcgtggtc tgatccacaa tgtaggtact gtcgcacacc
      961 atgcgcatca ggttgagatt gataagcaag ctttgtcggt ctttttcgtt gagaaaacca
     1021 aagcgctgcc atttagtcac caatcgggct acagcggcat tgaactcctc gtgcatgtct
     1081 ttttgcgcct gggtcatggg cacaaaaagt attttgtcca tcctctcagg caattgggaa
     1141 aggacttctt ttttggtccg ccgcagcaaa agccctttca acagttggga aatttcattt
     1201 aaattctgat accctacaat tttccccgtt tcgtttttta tttgataacg gttcagaaac
     1261 tgatagtaag gtccgagccg aaacgggtca ataaactgca caatggaata caattcttcc
     1321 agtttatttt cgagcggagt gccggtaagc accacacagt aaggcgtttt tatctttttt
     1381 accgctgcag caatttttgt tttccagttt ttaatacgct gtgcttcgtc cagcactatc
     1441 atatcaaaat cggcaacgtt tatagcattg atatcattta caaccgtgtg atacgaaact
     1501 attttgtaaa actcattacc cgtgtattgt tgtgctctga cattcggaag tccttcgata
     1561 acccgcaccg tgctatcgga gaaacgctca atttccgact gccactggta tttgagcgat
     1621 gtcggacaaa taataagcac acttgctatg tcgtgctctt tgcgtaacaa ttcggctgct
     1681 ccgatagcct gaatggtttt tcccaagccc atttcgtcgg caatcagtgc ccgtccggct
     1741 ttggctgcaa ataatacgcc ctggtgctga tatgggaaaa attcggtttt cagcaatccc
     1801 tgcagcgctt ccgactgcgg aaaaagttta tccagctttt cgctccgcct gttttgctct
     1861 ctgacgttga gcacaaactc catggcgtcg ttgtagcacc gaaaatctgc atcgagcgcg
     1921 tatgcttttt ccagaaaaac ttcaaaagac gaaaacgcat tggttttaag tacattttct
     1981 tcatcaaaaa acaattcggt gagctgctta aattcctttt catgatcggt tccaatgcga
     2041 attttgactt ttcgttcgcc tttataggag aggtaaaccg acgtatagga tattttataa
     2101 tctttcttta aataaccggc taatccgggt tgattttgga ttttccacaa tacggcttca
     2161 acatgcttac aggtgcccag acggttggtt ttgaagtcga gacacgagca gaaattcatg
     2221 gaattgcggt tgtcgcgtat cgaaactttg tacttattgc gtgtttcaaa attactaacc
     2281 tgataatcgc caaaaaccgg atgataccct tctatctttt cgataccaaa ggttttatcc
     2341 atcacaaact gctttcgcag agcaatttgc cagttttcca atgtcattcc ttccggtttt
     2401 ttatgatagg taacgctggt tttagctttt ggaattgggc gcagtttggt tgattttttt
     2461 gccatataat aattgtgtgt agtgtttaat gtgtaatgtt tattgtgtcc gtgcgtagct
     2521 gcgttttaat gcgcttaatt tatttgctat tttatctatt tgcaattgta tttcgtttat
     2581 ttgttctaat gcaatgtaat tcagatcgtt tgcaatttga agttgacaat aagtttccaa
     2641 taatgagcca taagctattt caataaatct ttctttttct ttgtctgatg ctcttgatgt
     2701 tccctcgg
//
LOCUS       DAOPZK010000210         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_54220_length_2664_cov_22.990418, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000210 DAOPZK010000000
VERSION     DAOPZK010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54220_length_2664_cov_22.990418"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKH68_09005"
     CDS             complement(<1..383)
                     /locus_tag="PKH68_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13112.1"
                     /translation="MKKTIQYLSLLLIVLTSFSLSISAQNKKADKDTRNWVYEIEPVG
                     VGTQGSYLIKVWSYSKKPMVALEQAKKNAVHGIIFKGFTGIQGVPGQRPLTNNPNLEV
                     EQAEFFKNFFAEGGKYMKFVNITNDG"
     gene            complement(419..2017)
                     /locus_tag="PKH68_09010"
     CDS             complement(419..2017)
                     /locus_tag="PKH68_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13113.1"
                     /translation="MVISKFRNLEVNSKFDNHKIDFPDLAPIDPLNLQKNALITKYIR
                     AASNPVIAKWWGRDADGNFNYSLVAQRGSYTATDADAIISRGSNTSRIEMIGEQLIDK
                     SYILLYEITDLYSMEEYYDRQDASNRKLKITTPVKRTEEGYNCDYKVYAYKVDFTDSV
                     ASEFYSRYWVDVKNHDNKKVAAWADAQFPVRLMASTGGSVRSAQSKQKTLNTKKKTMR
                     ELLEDIPAEIQNNAVFELGRKIEDFRLKVTVFKAYPVSAKLGTKEDLYIDQRFYVYEI
                     EQEKNGNQKINRKGVVRVKTINDNKQVATGSSLPSVFQQVAGGRIYQGMFMESKDDYG
                     IILNIGKNGSANNAMDGFNIGADFRISRFLKKPGWHFGIDASLRSMKDVYAGNISTPL
                     QPSLINSTELVSGGTYAIAVNISKESYFTRKGNVYLRPLVGLGLQSYSFNNIGGSEID
                     SENKDYSWSSIYVPAGIGVGWNITPTFSLEMKPGVYARFAATTGNKEKLTQSAAPVTD
                     NWGFGTIDKMNFGTTNTICLRIRF"
BASE COUNT          689 a          536 c          578 g          861 t
ORIGIN      
        1 ccgtcgttgg tgatattgac aaatttcatg tattttccgc cttcggcaaa aaagtttttg
       61 aaaaattcgg cctgttccac ttccaggttg ggattattgg tgagcggccg ttgaccgggc
      121 acaccctgaa tgcctgtaaa tcctttgaaa ataattccgt gtacggcatt ctttttggcc
      181 tgctcgaggg ctaccatggg ttttttggag taggaccata cttttataag gtacgaaccc
      241 tgagtaccca cacctaccgg ttctatttca taaacccagt tacgggtatc tttgtcggct
      301 tttttgtttt gtgccgatat gctcagactg aagcttgtga gtactatcag caagagactg
      361 agatactgaa ttgttttttt catgtctttc aaatttattg tttcaggata gaaaattatt
      421 aaaaccttat tcttaaacaa atggtatttg ttgttccaaa attcatttta tcgatggttc
      481 caaatcccca gttgtcggta acgggtgctg ccgattgggt gagcttttcc ttattgccgg
      541 ttgtagcggc aaagcgggca tacaccccgg gtttcatttc gaggctgaat gtgggagtaa
      601 tgttccaacc caccccaata ccggccggta cataaatgga tgaccaggag taatctttgt
      661 tttcagaatc aatttcgctt cccccaatgt tattgaacga gtaactttgc agcccgagtc
      721 caaccagcgg cctcagatat acatttccct ttcgggtgaa atagctttct ttggatatat
      781 tcacggcaat ggcataggtg cctcctgaaa ccaattcggt agaattaatc agtgatggct
      841 gcaagggggt gctgatatta ccggcataca catccttcat ggaacgcagc gaggcatcaa
      901 ttccgaaatg ccatcccggt tttttcagga aacgggagat gcggaagtcg gcaccgatat
      961 taaatccatc catggcattg ttggccgaac cgtttttacc tatattgaga ataattccat
     1021 agtcatcctt gctttccatg aacattccct ggtaaatccg cccgcctgct acctgctgga
     1081 aaaccgaagg cagggacgaa ccggtagcca cctgtttgtt gtcattgatg gttttcacgc
     1141 gcactacgcc tttacggttg attttctggt tgccgttttt ttcctgttct atttcataca
     1201 cataaaatcg ctgatcgatg tataaatctt cttttgttcc aagtttggca ctcacgggat
     1261 aagctttgaa cacggttact ttcagtcgga aatcctcgat ttttcgtccc aattcaaata
     1321 cggcattgtt ctggatttcg gcaggaatat cttcgagtaa ttcccgcatt gtttttttct
     1381 tggtgttcag cgttttttgc tttgactggg cagagcgcac cgacccaccg gtagaggcca
     1441 tgagacgcac cggaaattga gcatcagccc aggctgccac ttttttattg tcgtgatttt
     1501 tgacatcgac ccaatatcgg gagtaaaact cgctggctac cgagtcggtg aaatccactt
     1561 tgtaagcata taccttgtag tcgcaattgt agccttcttc ggtacgtttc accggtgtgg
     1621 tgattttcag tttgcggttg gaggcatcct gccggtcgta atattcctcc atggaataca
     1681 gatcggtgat ttcgtaaagc agaatatagg atttatctat cagttgttcg cctatcatct
     1741 ctatacggct ggtgttggag ccgcgtgaaa tgatggcatc ggcatcggtg gctgtatacg
     1801 aaccacgttg ggccacgagt gaatagttga aattgccgtc ggcatcgcgt ccccaccatt
     1861 tggctattac aggattggaa gctgcccgaa tgtattttgt gatcagtgca tttttttgca
     1921 gattgagcgg atcaatgggt gcaagatctg gaaaatcgat tttgtggttg tcgaacttgc
     1981 tgttgacctc cagatttcgg aatttggaga taaccacttc ttcgtcggca tctttgggct
     2041 atgagaacaa catggtaatg gaaggccgga gatatttggt attaactttt ccggttggat
     2101 tttgagcata aagaccggaa ataagcacca cacatgtaaa caggaatgga aatcgtttca
     2161 tgaatataaa ttttgggttg tcggattacg acaagaaggt taagaacaag agaacagaca
     2221 ggtatttcat gccggggaag gtcagcccgt attcttattg acctgagaat tatttttttt
     2281 tgaaaaaatc tataacttca gtgatgaaat gtcccttatt atttttggat taattaataa
     2341 tccaaaagca aaaatataca attatttgga tattggatgt tttttgtgca ataaagtgca
     2401 aattatttat ctgtgtaaaa tcataatgca ttaaactggt tcggcgtcag ttgaaaaata
     2461 cggataaagc ctgccaacca ttttgctgaa tgctgtaaaa agatttattg aattatcatt
     2521 tcaacattag tttcagatat atttaacatt tatcactttt attgtctgtt ttatcacaga
     2581 taatgatttt aagtgtaaat ttgcgccttt tattccaaat ctatttattc acaaacaatg
     2641 aaaaatattt tcacaattct ttta
//
LOCUS       DAOPZK010000211         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_54563_length_2654_cov_21.926895, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000211 DAOPZK010000000
VERSION     DAOPZK010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54563_length_2654_cov_21.926895"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKH68_09015"
     CDS             <1..899
                     /locus_tag="PKH68_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNX13114.1"
                     /translation="AEELGRTVGIIRDLFNSNFKHIYVNDLSVFNEVRDYVDLIAPER
                     KDIVRLYKDEMPIYDTFGITKQIQSALGKTVSFKSGAYLIIEHTEAMHVIDVNSGNRS
                     KAGNGQENNALDVNMAAADEIARQLRLRDMGGIIVVDFIDMHEGENRQKLYDKMRLMM
                     ENDRAKHNILPLSKFGLMQITRQRVRPVIDIDIEETCPTCYGKGRTQPSILFTDQLES
                     KIDYLVNTLKIKEFTLHIHPYVDAFVSKGLVSLKMQWKFKFGKGCKVIPSQKLGFLQY
                     KFYDKKGEEIDLQEEKEKISSK"
     gene            complement(956..1966)
                     /locus_tag="PKH68_09020"
     CDS             complement(956..1966)
                     /locus_tag="PKH68_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13115.1"
                     /translation="MTRSNSLVLTVLLLFSFTACNTPKEKSTKDGTTTEESSYEPIDT
                     TAYNQKLISLANGDTTGKWPVKNQPYPLAGAILPHSRIVAFYGNLYSKRMGALGEYAP
                     KEMWAKLNKEVKAWETADPTTPVIPALHYIAVVAQGDAGKDGKYRFRMPEKQIDSVFT
                     ISRMGPENTLIFLDIQVALSKIQDELPLLEKYLKLPNVHLGIDPEFSMKTGAKPGTKI
                     GTFDAVEINYCSQYLAKLVKDNNLPPKVFVIHRFTQGMVTNYKNIKLHPEVQIVIDMD
                     GWGEPELKKGTHRNYIHKEPVQFTGFKLFFKNDLKKSPNRMLTPEELLKLKPQPIYIQ
                     YQ"
     gene            2112..>2654
                     /locus_tag="PKH68_09025"
     CDS             2112..>2654
                     /locus_tag="PKH68_09025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNX13116.1"
                     /translation="MSDILLNTQLMLLDRAAFLQHREELVKLYLQSFTTGELAQYIPA
                     ETAIETLEELSLKGGGVIALRNEKIVGAIYGLPLAYDKEFPAEQCPEIPVDNTTYIAE
                     LMVHSELRGQGLASELMHTFLENEKMKGNSDAVIRVWDKNTGALNLYKKQGFSEIAEI
                     AQEKIQQDGMTVFQMNKIYLH"
BASE COUNT          773 a          522 c          596 g          763 t
ORIGIN      
        1 tagctgaaga attgggtcgc acagtgggaa taatcaggga tttgtttaat tccaacttta
       61 agcacatcta tgtgaatgat ctcagtgttt tcaatgaagt gcgcgattat gtggatttaa
      121 ttgctcccga acggaaagac atcgtaagat tatataagga tgaaatgcct atatacgata
      181 ccttcgggat tacaaaacaa atccagtcgg cactcggaaa aacagtttcg ttcaaaagcg
      241 gtgcttatct gattatagaa cacacagaag ccatgcatgt gatagatgtg aacagcggta
      301 accgctcgaa agccggaaac ggtcaggaaa acaatgcatt ggatgtgaat atggctgcag
      361 ccgatgaaat tgcccggcaa ttgcgattgc gtgatatggg cggaattatt gtggtggatt
      421 ttatcgatat gcacgaaggt gaaaaccggc agaagctgta cgataaaatg cgcctgatga
      481 tggaaaacga cagggccaag cataacattt taccgctgag taaattcgga ttgatgcaaa
      541 ttacccgtca gcgtgtccgg ccggtgattg atattgatat agaggaaacc tgtcctacct
      601 gctacggcaa gggacgcaca caaccctcga tactgttcac cgatcagctc gaaagtaaaa
      661 ttgattatct ggtaaatacc ctgaaaatca aagaattcac gctgcacatt catccctacg
      721 tggatgcatt cgtttccaaa ggacttgttt cgctcaagat gcaatggaaa ttcaaatttg
      781 gcaaaggctg caaagttatt ccttcgcaaa aactcggctt tctgcaatac aaattttacg
      841 ataaaaaagg agaagaaatt gaccttcagg aagaaaaaga aaagatttcg tctaagtaaa
      901 aaaaagagct gctcattcgg gcagctcttt ttgtgtaaaa tatatcagaa ttatcctact
      961 gatactgaat atatatcggt tgtggtttca gtttgagcag ttcttcgggt gtgagcatcc
     1021 ggttcggact tttcttcagg tcgtttttga aaaatagctt aaatccggta aactgaaccg
     1081 gttctttgtg aatgtaattg cggtgggtac ctttttttag ttccggttcg ccccagccat
     1141 ccatgtcaat cacaatctgc acttcgggat gcaatttgat gtttttatag ttggtaacca
     1201 tcccctgggt gaagcggtga ataacaaata ccttcggtgg cagattattg tctttcacca
     1261 acttggcaag gtactgcgag cagtagttaa tttccacagc atcaaaggta ccaatttttg
     1321 tacccggctt ggctcccgtt ttcatcgaaa attccgggtc aatgcccagg tgaacattcg
     1381 gaagtttcag gtatttctcc agcaggggca gttcatcctg aattttgctc aacgccacct
     1441 gaatatccag aaaaatcagt gtattttctg gccccattct cgaaatggta aaaaccgaat
     1501 caatctgttt ctccggcata cggaagcgat atttgccgtc tttaccggca tcgccctgcg
     1561 ccaccacggc tatgtaatga agtgccggaa tgaccggagt tgtcgggtcg gccgtttccc
     1621 aggctttaac ttctttattc agttttgccc acatttcttt gggtgcgtac tcgcccagcg
     1681 cacccattcg tttggagtac agattaccgt aaaaagccac gatgcgggaa tgggggagta
     1741 tagctcctgc cagcggatag ggttgatttt ttacgggcca cttgccggta gtgtcaccat
     1801 tggccagcga tattaacttc tgattataag cagtggtatc aatgggttcg taactgcttt
     1861 cttcggtagt tgtgccgtct tttgtacttt tttcttttgg agtgttacag gctgtaaatg
     1921 agaataataa caaaacggtg agaactaagc tattggatct tgtcatgaat tctaaatgag
     1981 gattaatgct gattttcttt agtcagcaaa attagctaaa ttattgtgaa taactaaatt
     2041 cctgatcaaa tatattctaa ataaaaggtt aattctaaac atccaaacag tttaattgtt
     2101 attctgcaac tatgtcagat atacttttaa atacgcaatt gatgctgctc gacagagccg
     2161 cttttcttca acaccgagaa gagctggtga aattgtattt gcagtcattc accaccggag
     2221 aactggctca atatattccg gccgaaactg cgattgaaac ccttgaagaa ttatctctca
     2281 aaggtggcgg tgtaattgca ttgcggaacg aaaaaattgt tggagccatt tacggcctgc
     2341 cgcttgctta cgataaagaa tttcccgctg aacaatgtcc tgaaattccg gtggataata
     2401 ccacgtatat tgctgaactg atggtgcatt ccgaactgcg agggcaggga ctggcttcgg
     2461 aattgatgca tacttttctg gaaaatgaaa aaatgaaggg aaactcggat gctgttattc
     2521 gggtttggga taaaaatact ggtgcactta atctttacaa aaaacagggt ttcagtgaaa
     2581 tagctgaaat tgctcaggaa aaaatccaac aggacggaat gactgttttt caaatgaata
     2641 agatatattt acat
//
LOCUS       DAOPZK010000212         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_54944_length_2642_cov_19.105528, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000212 DAOPZK010000000
VERSION     DAOPZK010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54944_length_2642_cov_19.105528"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..99)
                     /locus_tag="PKH68_09030"
     tRNA            complement(24..99)
                     /locus_tag="PKH68_09030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Pro,seq:ggg)
     gene            complement(185..>1108)
                     /locus_tag="PKH68_09035"
     CDS             complement(185..>1108)
                     /locus_tag="PKH68_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HNX13117.1"
                     /translation="RGLRNFDTALAYEAMTKSATLESARNLMRPDNDDYLQLGYVPLR
                     EKFDNSVSHALEYYLADWNLAQFAKSMGKKNDYQLFLNRSLNYKHYFDPETKILRPKL
                     PDGKFLVPFNPSQGANFEDVPGFHEGSAWNYAYYIPHDIKGLSKLMGGKKKFVDQLQM
                     VFDKGYYDMANEPDIAYPYLFNYFKGEEWRTQKEVTRLINKYFTNTPGGLPGNDDCGT
                     MSAWLIYSMMGFYPVCPGDMNYALTTPVFDKITIQLDPKYYKSAELIIEKKSTVTGTA
                     PVQQITVDSRKLDRFFISHDQLTSSKRLVYK"
     assembly_gap    1109..1208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1209..2252)
                     /locus_tag="PKH68_09040"
     CDS             complement(<1209..2252)
                     /locus_tag="PKH68_09040"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNX13118.1"
                     /translation="MRTRTFLLFTFILLTISINTFSQNLTRFVNPFIGTTNYGTTNPG
                     AVYPHGMVTVCPFNVTGSKENKFDKDAQWFSTPYEYTNTVFTGLSHISLSGVGCPELG
                     SLLLMPTTGKPDADIKNYGSKISRLKAEPGLFSCNIDRYNIQSQSSATLRTGISEFTF
                     PAGESNILLNLGEGLTNESGAFARIVSDTEVEGMKLLGTFCYNPQAVFPVYFVMKVSK
                     SPKRVVYWKKQRPMTAEAAWDNTAGTYKFYEKYNREIAGDDIGVIYNYETKADERITV
                     KIGISYVSIDNARKNLETEQPAFEVAKTINNARTEWESVLSKIKVEGGTHDQKTIFYT
                     ALYHLHIHPNILQD"
     gene            complement(2369..2572)
                     /locus_tag="PKH68_09045"
     CDS             complement(2369..2572)
                     /locus_tag="PKH68_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13119.1"
                     /translation="MSRNKLCPQCKIRRFQVKNDAGQSVVVMVSENNEIIPIHDDESL
                     EGFDLTVLYCLGCSWKGSVKSLI"
BASE COUNT          689 a          553 c          533 g          767 t
ORIGIN      
        1 gtgtaagtcc ttgattttca gtgtcggggt gacaagattc gaacttgcga ccactcgccc
       61 cccagacgag tactctaaac cgggctgagc tacaccccgt cgacattgaa ttgtagtgca
      121 aagataatat ttttttctta tttcaaaaaa gaatcagcac ttctggcgca aaattaatac
      181 cttattattt gtaaactaat ctttttgagc tggttaactg atcgtgagat atgaaaaacc
      241 ggtcaagctt tctgctatcc acggtaatct gttgaaccgg agctgtgccg gttacagttg
      301 atttcttctc aataatgagt tccgccgatt tataatattt aggatcaagc tgaatggtga
      361 ttttatcaaa aacaggagta gtcagtgcat aattcatatc gccgggacaa acggggtaga
      421 atcccatcat gctgtaaatg agccaggccg acatggttcc gcagtcatcg tttccgggca
      481 gacctccggg cgtgtttgtg aaatatttat tgatcaggcg tgtgacttct ttctgagtgc
      541 gccactcctc tcctttgaaa tagttgaaca gatacggata cgcgatatcc ggctcgttgg
      601 ccatgtcgta atagcctttg tcgaacacca tctgaagctg atccacaaat ttcttttttc
      661 cacccatcag ttttgaaaga cctttgatat catgcggaat ataataggca tagttccagg
      721 cactgccttc gtgaaatccg ggaacatctt caaagttcgc tccctgtgat ggattaaatg
      781 gaactaaaaa ctttccgtcg ggcaattttg gacgaagtat ctttgtttcc ggatcaaaat
      841 aatgtttgta attcagtgat ctgttaagaa acagttgata atcattcttt ttacccatcg
      901 atttggcaaa ttgcgccaaa ttccaatcgg ccagatagta ctccagcgca tgcgaaaccg
      961 aattgtcaaa cttttcacgc aaaggcacat aacccagttg cagataatca tcattatctg
     1021 gtcgcataag gttgcgcgca ctttccaggg tcgccgattt agtcatggct tcataagcca
     1081 gagcggtatc aaagttacga agtccacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnngt cctgcaatat attgggatga atgtgtaaat ggtacagagc ggtgtagaag
     1261 attgttttct gatcgtgggt tccaccttcc acctttattt tcgacagcac actctcccac
     1321 tccgtccggg cattatttat agttttagcc acttcaaaag caggttgctc tgtttccagg
     1381 tttttgcggg cattgtcgat gctcacgtac gatattccga tttttacggt tattctttca
     1441 tcggctttag tttcatagtt gtaaataaca ccaatatcat ctccggctat ttcccggtta
     1501 tatttctcat aaaatttgta cgttccggcg gtgttatccc aggcagcttc agctgtcatg
     1561 ggtcgttgtt ttttccagta aaccacccgc ttgggtgatt tgctcacctt cattacaaaa
     1621 tataccggaa acacagcttg tggattgtag caaaatgtac caagcagttt catcccttcc
     1681 acttcggtat cactcactat gcgggcaaaa gcgccacttt cgttggtcag accttcaccc
     1741 agattcagca gaatattact ttctccagcg ggaaaggtaa attcggaaat tcctgttcgc
     1801 agcgtggctg aactctgact ttgaatatta tatcggtcaa tgttacacga aaacaatccg
     1861 ggttcagcct tcaagcggct gattttactt ccgtagtttt ttatatcggc atccggctta
     1921 ccggtagtag gcatcagcag cagactaccc agttccggac agcccactcc gctcagactt
     1981 atatgtgata gtccggtaaa aacagtgttg gtatattcgt agggagttga aaaccactga
     2041 gcatctttgt cgaatttatt ttcttttgag cccgtaacat tgaatggaca aaccgttacc
     2101 ataccatgcg gatacacagc tccgggattg gtggtaccgt aattggtcgt accaataaag
     2161 ggattgacaa atcgggtgag attttgggaa aatgtattta tgctgattgt cagcaaaata
     2221 aaggtaaaga gaagaaaggt tctggttctc atagtcagat attcttttaa gggttttatt
     2281 gaatgggaat gtcagaatta caaaacattc cggttaacaa ttttcacaat gaaatatctt
     2341 tgccagttga acaaaatcag caaatcaatc agatcagtga cttcacagag cccttccagg
     2401 agcaaccgag gcaataaagc actgtcaggt caaaaccttc gagcgactca tcatcgtgta
     2461 tgggaatgat ttcgttattt tcactcacca tgacaactac agactgtccg gcatcatttt
     2521 tcacctgaaa gcgacggatt ttgcactgcg gacaaagttt atttctgctc attttcttat
     2581 ttaaaataaa atcccgtaaa cggtatgcgc ttcgggattg ttatattata gcggaaagcg
     2641 ag
//
LOCUS       DAOPZK010000213         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_56082_length_2606_cov_24.254018, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000213 DAOPZK010000000
VERSION     DAOPZK010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56082_length_2606_cov_24.254018"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1764)
                     /locus_tag="PKH68_09050"
     CDS             complement(<1..1764)
                     /locus_tag="PKH68_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNX13120.1"
                     /translation="MNNLKRSTPRTFSPWTWIPTLYFAEGIPYVVVMTVAVIMYKRLG
                     ISNTDIALYTGLLYLPWVIKPFWSPFVDLIRTKRWWIVTMQLILGAGLAGVAFLIPAP
                     FFFQATLAVLWLIAFSSATHDIAADGFYMLALNSSQQSFFVGIRSTFYRLAMITGQGV
                     LIILAGFLETSTGLEPLNLNVQSTTKTVQTDTWQRELAAAPAHSSEAVFTAFPAVLEI
                     NTLNISPDSLQSIKNYATTENLKNGFIQAEKKVEKQKTWWQRNVSEQWTKYVALPFGN
                     FISRNFGKKTEISLTSAGEAGNAGIIAVKLNKKPEEGKETILNTRLLSGDKSIALVSG
                     ERLAFNAQNWDKPAYLVVQLDRKLNTEVKATFRGTSGNLPLAWSITFYILAGFFLLLF
                     IYHRFVLPKPASDAPKNHSPKDIFIEFGQTFATFFRKRGIGVAIAFMLLYRLGEAMLV
                     KLSSPFLLDGREIGGLGLTTGQVGLVYGTVGIVALTLGGIVGGIAASRKGLKFWIWPM
                     ALCITLPHLAYLYLSWYMPENMLLINAAVAVEQFGYGFGFTAYMLYLIYFSDGEHKTA
                     HYAICTGFMALGMMLPGMFAGW"
     gene            2089..>2606
                     /locus_tag="PKH68_09055"
     CDS             2089..>2606
                     /locus_tag="PKH68_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNX13121.1"
                     /translation="MKKILSIVLIALITLTAGFSGSALSAAKVVKPIKTATPARPKGQ
                     TDVVNLVTPKLDVVRVGFIGLGMRGPGAVERWTHIPGVKIVALCDLLPERVAGSQKIL
                     EKAGLPKAAEYSGTEDAWKQLCERDDIDLVYIVTDWKHHADMALYAMQHGKHTAIEVP
                     AAMSMKEIWALI"
BASE COUNT          783 a          606 c          577 g          640 t
ORIGIN      
        1 ccaaccggca aacataccgg gcaacatcat gcccagtgcc ataaatccgg tacaaatagc
       61 gtaatgtgcc gttttgtgct ctccatcgga aaaataaatc aggtaaagca tgtaagcagt
      121 aaaaccaaaa ccgtatccaa actgctcgac cgccacagca gcattaatca acagcatatt
      181 ttcgggcata taccacgaca aatacagata agccagatgg ggcagtgtaa tgcagagagc
      241 catcggccaa atccagaatt tcaaaccctt gcgcgaagca gctattccac ccacaatacc
      301 tccaagtgtc aatgctacaa tacctaccgt gccatagacc aatcccactt gtccggtggt
      361 aagtccgagc ccgcctattt cgcgcccatc gagcaggaac ggcgacgaca atttcacgag
      421 catagcttca cccaagcggt agagcaacat gaacgcaatg gcaacgccga tacctctttt
      481 tctgaaaaat gtagcaaatg tctgtccgaa ttcaatgaaa atatctttgg gagaatgatt
      541 ttttggtgca tcggaagcgg gttttggcaa cacaaaacgg tgatagataa acaacagcag
      601 gaagaaacct gccagaatgt aaaaggtaat actccaggct aagggaagat tgccggaagt
      661 gccccggaaa gtcgctttta cttccgtgtt gagtttgcgg tcgagctgaa ccaccagata
      721 agccggtttg tcccagttct gagcattaaa ggcaagtcgc tcaccactca ccagcgcaat
      781 gctcttgtcg ccagacaaca ggcgggtatt gagtatggtc tcctttccct cttcgggttt
      841 tttattcagt tttacggcaa taattccggc attacccgct tctccggccg atgtaagaga
      901 tatttccgtt ttctttccga aattgcgact gataaaattc ccaaatggta gtgctacgta
      961 tttcgtccat tgctccgaca cattccgttg ccaccaggtt ttttgctttt ctaccttttt
     1021 ttcagcctga ataaatccgt ttttaagatt ttcggtggtg gcatagtttt taatcgactg
     1081 cagcgaatcc ggactgatat tcagcgtatt gatttcaaga actgcgggaa atgccgtaaa
     1141 tactgcttct gatgaatgtg caggagcagc agccagttcg cgctgccatg tatcagtctg
     1201 aaccgtttta gtagtggact gcacattgag gttaagcggc tcaagtccgg tgcttgtttc
     1261 gaggaaacca gccagaataa tcaacacacc ctgccctgta atcatggcca gccggtaaaa
     1321 tgtgctacgg ataccaacaa aaaaagattg ctgtgaactg ttgagagcta acatataaaa
     1381 tccatcggca gcaatatcgt gggtagccga actgaaagca atgagccaca ggacagccag
     1441 tgtagcctga aaaaagaaag gcgccggtat gagaaacgct actcctgcca gtccggctcc
     1501 caaaatgagc tgcatggtca caatccacca gcgctttgta cggatcagat ccacaaacgg
     1561 actccagaaa ggctttatca cccagggtaa atacaacaaa ccggtataaa gggctatgtc
     1621 ggtattggaa atgcccagcc gcttgtacat aatgacagct accgtcatca ctacaacata
     1681 gggaatccct tcggcaaagt aaagagtggg tatccaggtc cagggagaaa aagttcgggg
     1741 agtggaacgt ttgaggttat tcatgggtat ggggactgaa cggttaataa ttcatcacaa
     1801 aattaatgaa ttagaccgga taaacagaat gaataacaca tttttcaaca aaaactcact
     1861 gttttattgt tatgagtgca cttgcatgac accggtatca ttacggtcat tggtcaacac
     1921 aaaaagaaat aatagcaaaa tttcagttgc atctgaatag gttctgttaa gattaacagg
     1981 aataaaattg gttttattcc gatggaaaga gaatagttat ttcagatatt ttggttattt
     2041 ttgcaacctg aatcaacctt acagtcaata atctataaat aaaaatctat gaaaaaaatt
     2101 ttaagtattg ttttgattgc actgatcacc cttacagccg gcttctccgg ctcggcactc
     2161 agcgcagcca aagtagttaa acccatcaaa acggccactc ccgcccgtcc gaaaggacaa
     2221 accgacgttg taaacttagt aacaccaaaa ctggatgtag tccgcgtagg cttcatcggc
     2281 cttggcatgc ggggtcccgg tgctgtagaa cgctggacgc atattcccgg tgtaaaaatt
     2341 gttgctcttt gcgatttact tcccgaacgg gtagcaggca gtcagaaaat actggaaaaa
     2401 gcaggattgc caaaagctgc cgaatattcc ggtaccgaag atgcctggaa acaactttgt
     2461 gaacgcgacg atatcgacct tgtgtacatt gtaaccgatt ggaaacacca tgcagatatg
     2521 gctctttatg ccatgcaaca cggtaagcac acagccattg aagtgcctgc tgccatgagc
     2581 atgaaagaaa tctgggcgct catcaa
//
LOCUS       DAOPZK010000214         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_56403_length_2596_cov_19.759543, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000214 DAOPZK010000000
VERSION     DAOPZK010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56403_length_2596_cov_19.759543"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..>2596
                     /locus_tag="PKH68_09060"
     CDS             201..>2596
                     /locus_tag="PKH68_09060"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNX13122.1"
                     /translation="MKRAVMLILASFFVSVSSFASEADIAIPDLHAGTFHIFGQEITS
                     WNFLFYGALVICFTLGISLWLRHQVKREKAHVSMLKVSEIIFQTCKTYLLQQGKFLLM
                     LFGFVAVIMAYYFLVLQGKPMGTLLFVLCFSILGMGGSYAVAWYGIRINTFANSRTAF
                     ASLRGKPWDVVNIPLRSGMSVGLFLISLELVMMVVILLFVPRDIVGICFLGFAIGESL
                     GASVLRIAGGIFTKIADIGSDLMKVVFKVKEDDPRNPGVIADCTGDNAGDSVGPTADG
                     FETYGVTGVALITFITLAVHNPDIQAKLIVWIFGMRFLMDLCSGLSYFINQGISKRKY
                     KGMKIFDFEEPLMRLIWIAAILSISTSFFMSYLLVGDLADPSLWWKLAGIISCGTLAA
                     VLIPEFTKLFTSSKSAHVGEIVTASREGGPSLNILSGIVSGNFGAFWTGLLIVALMTG
                     AYFISVFGHLEAVLGPHATIFAFGLVAFGFLCMGPVTIAVDSYGPVTDNAQSIFELSQ
                     IEQIPGIKEEIEKDYGFTPDFELGKHYLEANDSAGNTFKATAKPVLIGTAVVGATTMI
                     FSIILLLESVGMLSVSLTSAPILLGLICGGAVIFWFSGASMQAVTTGAYRAVEYIKRT
                     FKLENAEANMKDSVEVVKICTQYAQKGMWNIFIALICFTIGFAFFDSNFFVAYLISIA
                     CFGLFQAIYMANAGGAWDNAKKVIEVDLKEKNTPLHEAAVIGDTVGDPFKDTSSVSLN
                     PIIKFSTLFGLLATEIALKMKLDAMKGLGTDYSGYIGVVFLIIALVFVWRSFYKMRIP
                     L"
BASE COUNT          649 a          530 c          587 g          830 t
ORIGIN      
        1 cgaaaatcta tctgtgattt tcgggacttt ttatttgatc ctaagttcat cattagtttt
       61 attacaatac ttacactcaa ttatttaaca catatttaaa cttttctgta aaaatatctc
      121 aattaattat tactaacttt ggtcgttttt tttcattttc aaaccattta aaatcaaact
      181 acacattacg catgaaaaac atgaaaagag cagtaatgct catccttgca agtttctttg
      241 tgtcagtcag ttcctttgcc agcgaggctg acattgccat tcccgactta cacgctggca
      301 cctttcacat tttcggacag gagattactt cctggaattt tttattctac ggagcgctcg
      361 ttatctgctt taccctgggc atcagcttgt ggctgcgcca tcaggtcaaa agggaaaaag
      421 cacacgtttc gatgttaaaa gtttcagaaa ttatttttca aacctgtaaa acgtacctgc
      481 tacaacaggg aaaatttctt ttaatgcttt ttggttttgt agcagtaatt atggcttact
      541 atttcctggt actacagggc aagccgatgg gtacgctatt gtttgtgctt tgtttctcta
      601 tattaggtat gggtggttct tacgccgtgg catggtatgg catccgtatc aatacttttg
      661 ccaattcgcg cacggcattt gcttcccttc gcggaaaacc gtgggatgtg gttaatattc
      721 cgttgcgttc gggtatgagt gtgggacttt tcctcatttc acttgagtta gtgatgatgg
      781 tggtgatcct gctgtttgtt ccacgcgaca ttgtaggtat ttgtttcctt ggatttgcca
      841 tcggtgaatc attgggagcc agtgtacttc gtattgccgg aggtattttt accaaaatag
      901 ccgatatcgg ttccgacctg atgaaagtgg ttttcaaggt caaagaagat gacccccgta
      961 atccgggtgt aattgccgac tgtaccggtg acaatgccgg cgacagcgtt ggccctactg
     1021 ccgatggatt tgaaacctac ggcgtaaccg gcgtggctct cattaccttt ataacattgg
     1081 ctgttcataa tcccgacatt caggcaaaac tcattgtttg gatatttggt atgcgtttcc
     1141 tcatggactt gtgttccggt ctctcctatt ttattaatca gggcatttcg aaacggaaat
     1201 acaagggaat gaagatattt gactttgaag aaccgctgat gcgtcttatc tggattgcag
     1261 ctatcctgag tatttccact tcattcttta tgagttattt actggtgggc gacctggccg
     1321 atccgtcgct gtggtggaaa ctggccggta ttatcagttg cggtacactg gcagcagtgt
     1381 taatccccga atttaccaaa ctgtttacca gttccaaatc agctcacgta ggtgagattg
     1441 ttacagcatc tcgtgaaggc ggaccatcat taaatatcct ttcgggtatc gtatcgggta
     1501 atttcggtgc gttctggaca ggtttactga ttgtagcctt gatgactggt gcctatttta
     1561 tcagcgtatt cggacacctg gaagctgttt tgggtcctca cgctactatt tttgcgtttg
     1621 gtctggtggc tttcggattt ctgtgtatgg gtccggtaac tatagccgtg gacagctacg
     1681 gcccggtgac cgacaatgct cagtctattt tcgaattatc acaaatagag cagattccgg
     1741 gtattaagga agaaatagaa aaagattatg gttttacacc cgattttgaa ttgggtaaac
     1801 actacctgga agccaacgat agtgccggca atacgttcaa agcaaccgca aagccggtat
     1861 tgatcggtac agctgtagtt ggtgcaacta ctatgatttt ctccatcatc ctgttgctgg
     1921 aaagtgtggg aatgctcagc gtttcgctaa catctgcacc cattctgctg ggacttatct
     1981 gtggtggcgc agtaatattc tggttcagcg gagcatccat gcaagccgtt accactggag
     2041 cttaccgcgc agtggaatat atcaaacgaa cattcaaact ggaaaatgcc gaagcaaata
     2101 tgaaagactc ggtagaagtt gtaaaaattt gtacacagta tgctcaaaaa gggatgtgga
     2161 atatctttat cgccctgatt tgttttacaa taggctttgc tttctttgac agcaatttct
     2221 ttgtggctta tctgatttcc attgcctgct tcggattatt tcaggcaata tatatggcta
     2281 atgcgggcgg tgcctgggac aatgccaaga aagtgataga agtggatctg aaagagaaaa
     2341 acacaccgtt gcacgaagca gctgtgattg gcgataccgt gggcgatcct ttcaaagata
     2401 cttcctcagt ttcattgaat ccgatcatca aattctctac cctgtttggt ttactggcta
     2461 ccgaaattgc attgaaaatg aagctcgatg ccatgaaagg acttggcaca gattatagcg
     2521 gatatatcgg agtggtattc ctgattattg cgctggtttt tgtatggcga tcattctaca
     2581 aaatgagaat tccact
//
LOCUS       DAOPZK010000215         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_57404_length_2567_cov_24.247213, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000215 DAOPZK010000000
VERSION     DAOPZK010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57404_length_2567_cov_24.247213"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..753
                     /locus_tag="PKH68_09065"
     CDS             202..753
                     /locus_tag="PKH68_09065"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNX13123.1"
                     /translation="MSGKYEDLEKLKDLLEKGILSETEFITEKEKILGKEVNGNQKTT
                     QQISTKDNNSFCALMHLSQFANYLLPPVGLLAPIVMWLTRRNESTAVDVNGKIILNWT
                     ISVFIYSIVIVIGFLIFGATSILTIINYDNPLPLIGTIGIFLIPLIIFGILDLVFVIV
                     GAIKANNGEVWNYPLSIRFFNTK"
     gene            799..1464
                     /locus_tag="PKH68_09070"
     CDS             799..1464
                     /locus_tag="PKH68_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNX13124.1"
                     /translation="MIRLTNINKTYYTEATSLHVLKGIDMTIDTGEFVAITGASGSGK
                     STLLNILGILDNYDTGDYYLNEMLIKNLSEKQAARYRNEMIGFVFQSFNLINFKNALE
                     NVALPLYYKNISRKKRNIIAMEYLDKMGLKDWATHMPSEMSGGQKQRVAIARAIISNP
                     KVLLADEPTGALDSKTSLEVMDVLKELNKSGITTIIVTHEQSVAETADRIIHIKDGLI
                     NNN"
     gene            1477..>2567
                     /locus_tag="PKH68_09075"
     CDS             1477..>2567
                     /locus_tag="PKH68_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNX13125.1"
                     /translation="METIEEIISTLKHNKMRTLLTGLSVSWGIFILIVLLGAGNGLQN
                     GVISNFSDRATNTVQMWPGRTTKPYKGLKTNRQLNFTEKEIGNLKENVKESDEETGII
                     EKSVTVSYKTEFGNYQLKGVEPSYSQIINLKFEPGDGRFINSLDITNNSKVIVIDKKV
                     EETLFKGKSALGEYVKVGSVMFRVIGINSKKEQFAGTQVYIPFKTAQMIYNPDKKFWS
                     IAFTTTGLKTKQANEDFDENLKKTMSQSMFFDPKDEQALWIRNSQRDYIETMNIFNGI
                     NIFVSIIGIFTLIAGIVGVSNIMLVSVKERTREIGIRKAIGAPPANILRSIVLEALLI
                     TALFGYIGMMMGIGLTEVVSFIMEKGASQ"
BASE COUNT          877 a          500 c          506 g          684 t
ORIGIN      
        1 tttcttctat tcgtctatat atctgtagat tataaattct aaaaatataa tactatattt
       61 ttatatggta ctatgtattg catagtatta tatttcataa tatatttgca gcgttattga
      121 acaggattaa caaaaaaact gttaccaaaa agaccaaagt ttttaaacac tatcaatatt
      181 aatccttaat gtataattaa tatgtcaggc aaatacgaag atttagagaa actgaaagat
      241 ctcctggaaa aagggatact atcagaaaca gagtttatca ctgaaaaaga aaaaattctt
      301 ggcaaagaag tgaacggaaa tcagaaaact actcaacaaa tttctacgaa ggacaacaac
      361 agtttttgtg ctctgatgca tctctcccaa tttgccaact atctgttacc cccagtggga
      421 ctgttagctc ctatagtgat gtggctcacc cgccggaatg aaagcacagc cgtggatgta
      481 aacggtaaaa ttattctgaa ctggacgata agtgtgttta tctacagcat agtcattgtt
      541 atcggatttc tgatttttgg cgccacgtct atcctgacca ttatcaacta tgacaatccg
      601 ctgcctttaa tcggtaccat cggcattttt cttattccac ttatcatttt cggaattctt
      661 gatctggtat ttgttatagt aggcgctatc aaagccaata acggcgaagt atggaattat
      721 ccgttaagca tccgtttttt caatacaaaa tagcatttat caactcagta aagatttatt
      781 ttcaaaacaa aagaaataat gataagactt actaacatca acaaaaccta ttacaccgaa
      841 gcaacttcgc ttcacgtgct gaaaggtatt gacatgacca ttgacaccgg agaatttgta
      901 gccattacgg gcgcatcggg ttcgggaaaa tctacgttgc tcaatattct gggaatactg
      961 gacaactatg ataccggcga ctattacctg aatgaaatgc tgataaaaaa tttatcggaa
     1021 aagcaggcag cccgctaccg caacgaaatg atcggttttg tttttcaatc tttcaatctg
     1081 attaatttca agaatgcgct ggaaaatgtg gctctcccac tctactataa aaatatcagc
     1141 cggaaaaaaa gaaatatcat agccatggag tacctggaca aaatggggct gaaagactgg
     1201 gcaactcaca tgccttcaga aatgtcggga ggtcagaaac aacgcgtagc gatagccaga
     1261 gccattattt caaatccaaa agtactgctt gccgacgaac ctaccggtgc tctcgacagc
     1321 aagacttcac tggaagtgat ggatgtactg aaagaactca ataaaagcgg aattacaacc
     1381 attatcgtaa ctcacgaaca atcggtagcg gaaacggctg accggatcat tcacattaag
     1441 gatggtctca tcaacaataa ttaattcaga cttctaatgg aaacaattga agaaataatc
     1501 tccactctca agcataacaa aatgcgcacg ctgcttacag ggctttctgt ttcctgggga
     1561 attttcattc ttattgtatt gctcggtgcc ggaaatggcc tccagaatgg cgtgataagc
     1621 aacttcagcg accgtgcaac caatacagta caaatgtggc ccggcagaac cacaaaacca
     1681 tataaagggc tgaaaaccaa cagacaactg aattttaccg aaaaagaaat cggaaacctg
     1741 aaggaaaacg taaaagagag tgacgaagaa accggaatca ttgaaaagtc ggtaacagta
     1801 agttataaaa cagaatttgg aaattaccag ctaaagggag ttgaaccaag ttattcccaa
     1861 attattaatc tgaaatttga acccggcgat ggtcggttta tcaattcgct cgacatcact
     1921 aataacagta aggttattgt catcgataaa aaagtggaag aaacgctctt caaaggaaaa
     1981 tcggcgctgg gcgagtatgt aaaagtgggg tcggtcatgt ttcgggtaat cggtatcaat
     2041 tcaaaaaaag agcagtttgc cggcactcag gtttacattc cttttaaaac agctcagatg
     2101 atttacaatc ccgataaaaa attctggtcc attgcattca ccaccacagg attgaaaacc
     2161 aaacaggcga atgaagattt tgatgaaaac ctgaaaaaaa ccatgtcgca aagtatgttt
     2221 tttgatccga aagatgagca ggcattgtgg atacgaaatt cacaacggga ctacatcgaa
     2281 acgatgaata ttttcaacgg tatcaacatt ttcgtaagta taatcggcat ttttacgctt
     2341 atagccggca ttgtaggtgt gagcaatatc atgcttgttt cggtcaagga acgaacgcgc
     2401 gaaattggta tacgcaaggc catcggagca ccaccggcaa atattctgcg ttccatagta
     2461 ctcgaagcac tgctgattac ggcacttttt ggctacattg gcatgatgat gggaatagga
     2521 ttaaccgaag ttgtcagctt cataatggaa aaaggcgcat cgcaaaa
//
LOCUS       DAOPZK010000216         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_57544_length_2563_cov_21.255183, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000216 DAOPZK010000000
VERSION     DAOPZK010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57544_length_2563_cov_21.255183"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PKH68_09080"
     CDS             <1..1035
                     /locus_tag="PKH68_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNX13126.1"
                     /translation="INPAFNKELEKFGSVNYNACYNCGTCTAVCSLSTEEVSFPREMV
                     RLSALGLEDDIKASLKPWECYYCGECSTHCPQEANPGELMMSLRRYLTSKYDWTGLSG
                     LMYKSLPASLVAFLLVFAGVMAFAFSVGFDLEKMVHTGHLFEGIAIGTIGLLILLPNV
                     ARMWYFTILKPKVKVPFKKYVKSMGELFVHMFTQKRALGCDDNQLRWFEHFILVIGYL
                     SLLFTTVVLNWFGTSSLFVIILGYVMSAIVFVVTIDFVSDRLKKKKELSKHSQPSDIF
                     FVVWLFLMGFTAFLVRLFIDLDILEGNKWLYIFHLTVLVQWALIIVPFGKWTHFLYRS
                     FAMYFAKLKE"
     gene            1321..2031
                     /locus_tag="PKH68_09085"
     CDS             1321..2031
                     /locus_tag="PKH68_09085"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNX13127.1"
                     /translation="METNEIITLPRIGDKAPSFKAVTTQGNINFPEDYAGKWAILFSH
                     PADFTPVCTSEFMTFASMQQEFDALNTQLVGLSVDGLYSHIAWLRTIKEKIEYKGMKN
                     VEVNFPLIEDITMEVAKKYGMIQPGESNTKAVRAVFFIDPKGIIRAMIYYPLSMGRNF
                     DELKRVIIALQTADNFGVATPADWRPGDDVIVPTAGSCGVAKSRMEGQEEGVTCYDWF
                     FCTKKLSKDKVEAALGKK"
     gene            2189..>2563
                     /locus_tag="PKH68_09090"
     CDS             2189..>2563
                     /locus_tag="PKH68_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNX13128.1"
                     /translation="MPRPKQDRKISNPPLMQGFKPFGIPRRMLSQVSLLYDEYEAIRL
                     LDYEGMNQDQAAVQMNVSRPTLTRIYENARKTIAQALVEGKMIMIEGGNVQFDRAWFR
                     CRRCYKLIGGLENHVHCKDCKTF"
BASE COUNT          756 a          506 c          556 g          745 t
ORIGIN      
        1 ataaatcctg cttttaataa agaactcgaa aaattcggct cggtcaatta caacgcatgt
       61 tataactgcg gtacctgtac agccgtttgt tccctgtcaa ccgaagaagt gtcctttcca
      121 cgcgagatgg ttcggttgag tgctcttggt ctggaagatg atatcaaagc ttcgctgaaa
      181 ccctgggaat gttactattg cggcgaatgt tccacgcatt gtccgcagga agctaatccg
      241 ggtgaactga tgatgtcgct tcgcagatat ctgacttcaa aatacgactg gacaggtctt
      301 tcgggactga tgtacaaatc actgccggca agtctggttg catttctcct ggtttttgcc
      361 ggagtaatgg cattcgcctt ttcggtgggt ttcgacctgg aaaaaatggt gcataccggt
      421 cacctgttcg aaggaattgc tatcggcact atcggactgc tgattttgct gcccaatgtg
      481 gcgcggatgt ggtactttac cattctgaaa ccaaaagtga aagtgccgtt taagaagtat
      541 gtgaaaagca tgggcgaact tttcgttcac atgtttacac aaaagcgcgc ccttggttgc
      601 gacgataacc aacttcgctg gtttgagcat tttatcctgg taataggtta cttgtcgtta
      661 ctgtttacca ccgtagtgct caactggttc ggaacttcga gcctgtttgt aatcatcctg
      721 ggatatgtaa tgagtgccat cgtgtttgtg gttactatcg attttgtttc agaccgcctg
      781 aaaaagaaaa aagaactgag caaacattca cagccttccg atattttctt cgtggtatgg
      841 ttgttcctga tgggcttcac agcattcctg gtacgtctgt tcatcgatct cgatatcctc
      901 gaagggaata aatggttgta catttttcat cttaccgtac tggttcagtg ggcattgatc
      961 atcgtgccat tcggtaaatg gactcacttc ctgtatcgct cgttcgccat gtactttgca
     1021 aagctgaaag agtaatcatc ggattgataa ttttccaaaa aagaagagag acgggcaaat
     1081 cgtttctctt ttttatgtta aaatgaatta tttagtgaac ataagttcat ttgatttgtt
     1141 ataatttcat attcgttttt tggcaaaaaa ataaggattg aattagtaat tctaagtaaa
     1201 atcaattaat acattaattt caaagatctg atatataaat atttaggttt gattttttgt
     1261 caaacaaata catgaacgaa aaatttataa aataaataat taacaaaaaa atcaagacaa
     1321 atggaaacaa acgaaattat tactttgccg cgtattggcg acaaagcacc ttctttcaaa
     1381 gcagttacca ctcagggcaa cattaatttt cccgaagact atgcaggtaa atgggctatt
     1441 cttttcagtc atccggccga ctttacaccg gtatgtactt ctgaatttat gacttttgct
     1501 tctatgcaac aggaattcga tgcactgaat acacagttgg tgggcctttc agttgatgga
     1561 ttgtacagcc acatagcctg gctgagaacc attaaggaaa aaattgagta caaaggaatg
     1621 aaaaacgtgg aagtaaattt cccgctcatc gaagacatta ccatggaagt ggccaaaaaa
     1681 tatggcatga tacaaccggg cgaaagcaac acaaaagcag ttcgtgccgt attctttatc
     1741 gatcccaaag gtattatccg cgccatgatt tactatccgc tttcgatggg acgtaatttt
     1801 gacgagctga aacgtgttat cattgcactt caaactgctg ataattttgg tgtggcaact
     1861 cctgccgact ggcgtccggg cgacgatgta attgtaccaa ctgccggatc atgcggcgtg
     1921 gccaaaagcc gtatggaagg tcaggaagaa ggcgtgacgt gttacgactg gttcttctgt
     1981 accaaaaaat tgtcgaaaga caaagtggaa gcagcactgg gtaaaaagta aaaactaaaa
     2041 cttactgata caaaagggag aaacggaaac actgtttttc ccttttttta tgaataaatt
     2101 tttcaacaaa acgaacataa gttcataatt atttctttat ctttgtcatt cgataacccg
     2161 ataaattcat tttcaaaaaa tcagatttat gccacgaccc aaacaggacc gtaaaatatc
     2221 caatccaccc ttgatgcagg gatttaaacc ttttggcatt ccccgtagaa tgctgagcca
     2281 ggtgtcattg ctttatgatg aatatgaagc catccggctg ctggattacg aagggatgaa
     2341 ccaggatcag gcagcagtgc agatgaatgt ttcgcgtccc acgttgaccc ggatatacga
     2401 aaatgcccga aaaaccatag ctcaggcact tgtagaagga aaaatgatca tgatcgaagg
     2461 tggaaatgtg cagttcgaca gagcctggtt tcgttgccgc cggtgctaca aactgatagg
     2521 tgggcttgaa aatcatgtgc attgcaaaga ttgtaaaact ttc
//
LOCUS       DAOPZK010000217         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_57843_length_2554_cov_31.626251, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000217 DAOPZK010000000
VERSION     DAOPZK010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57843_length_2554_cov_31.626251"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PKH68_09095"
     CDS             complement(<1..1534)
                     /locus_tag="PKH68_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNX13129.1"
                     /translation="MKEKIISIVESYGKDAYRLMDILSCVQSEFGCVSEEAVGVIAEQ
                     LHISEVDVIQTRSFYHFYTSRPVGKYGIYLNNSVVSCMMGMADVADEFEKQLGIRFGE
                     STPDKLASLHYTACIGMSDQEPAAIINDVVFTSLTREKVKNIILGMRAGKAAAEMVAE
                     YGDGNNAHALIKAMVKNNIKKTGPVIFDAHQTGAGLTKALNMDPKSVIGEIKESNLRG
                     RGGAGFPTGLKWDFCSRESGDKYVLCNADEGEPGTFKDRVLMTERSEMLFEGLAIGGF
                     AIGAKEGILYIRKEYKYLKAFLENTLKEMYAKNLLGKNILGKGFDFDIRIQFGAGAYV
                     CGEESGLIESCEGKRGEPRNRPPFPAQKGYKSKPSIVNNVETFCSVARILDKGGAWHS
                     TLGTKESSGTKVISVSGDCKTPGIYEIQWGMSIKEMLEMVGAEDVQAVQVAGPSGICL
                     APSQFDRKIALEDLPTGGSMIVIGKNRDLLKDVVLKFMEFFADESCGSCVTCRSFNMI
                     LKK"
     gene            complement(1921..>2554)
                     /locus_tag="PKH68_09100"
     CDS             complement(1921..>2554)
                     /locus_tag="PKH68_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HNX13130.1"
                     /translation="NACVELCPAEAISKNPLTLNLGQCVFCGECVSCKEDIIRFSNNF
                     RIATNNSSRLTVTSNTDHIIFDPELVRKEITKVFGRSLKLRLVSDGSCNGCELELNAS
                     DNVNFDVSRYGIEFVASPRHADGIVVTGPLVENSAKALELTWEAVPDPKILVAVGTCA
                     ISGGVFKNSKALNRGFFEKNTPDLYIPGCPPHPLTIIDGLLKLTGRITAD"
BASE COUNT          735 a          586 c          494 g          739 t
ORIGIN      
        1 attttttcag aatcatattg aaagaacggc aagtaacaca tgagccgcaa gattcgtcgg
       61 caaagaattc catgaatttc agcacaacat ctttcaacaa gtcgcgattt ttaccgatta
      121 caatcatgga gcctccagtt ggcaaatctt ccagagctat tttcctgtcg aattggctgg
      181 gtgccaaaca aattcccgag ggccctgcta cctgaacggc ctgaacatct tcggctccaa
      241 ccatttcgag catttctttg atgctcatgc cccattggat ttcataaatg ccaggagttt
      301 tgcagtcgcc cgacacactg attactttgg ttcctgaaga ttcttttgtc cctagggtgc
      361 tgtgccatgc accaccctta tccaatatcc tggccaccga acagaaagtt tccacattat
      421 ttacaatgga tggcttgctc ttatagcctt tctgggcagg gaaaggaggg cggttgcgcg
      481 gttcgccgcg tttgccttca caggattcta tcagcccaga ttcttcacca caaacatatg
      541 cgcctgcgcc aaactgtatc ctgatatcaa agtcaaaacc tttgccaaga atatttttac
      601 cgaggagatt tttggcatac atttctttca gtgtgttttc caaaaatgcc ttcaggtatt
      661 tgtattcttt tcttatgtat aagataccct ctttagcccc gatggcaaaa ccgcctatag
      721 ccaatccttc gaacaacatt tcggaacgtt cggtcatcaa caccctatct ttaaaagtac
      781 caggttcacc ttcatcggca ttgcataaca catatttatc acctgattcg cgggagcaga
      841 aatcccattt taaaccagtt ggaaatccgg cgccgccacg tccccgcaaa tttgattctt
      901 taatctctcc tatcacggat ttggggtcca tattaagggc tttggtaagt cctgcgccgg
      961 tttgatgggc atcaaaaata accgggccgg tttttttgat attatttttt accattgcct
     1021 ttatcaaggc atgtgcatta ttgccgtcgc cgtactcggc aaccatttcg gcagcagcct
     1081 ttccggcacg catacccaaa ataatatttt ttactttttc acgggtgagg gaggtaaaaa
     1141 ccacatcatt aataattgct gccggctcct gatcgctcat accgatacaa gcggtataat
     1201 gcagactggc taatttatcg ggtgtagatt caccaaaccg aataccgagt tgtttttcaa
     1261 attcatcggc aacatcagcc attcccatca tacaggaaac tacgctgttg ttgagataaa
     1321 taccatattt tccaaccggc cggctggtgt aaaaatgata aaacgaacgg gtttggataa
     1381 catcaacttc ggatatgtga agctgctcgg caatcactcc cacggcttct tcggaaacac
     1441 aaccaaattc agactgaaca caggacagga tatccatcag gcgataggca tcttttccgt
     1501 aagattcgac aatactgata attttttctt tcatagatgt tgggtttgtt tatgattttg
     1561 atggttttac aaaatatctt tttaagggac aaatttagta ataatttaat tctatcacaa
     1621 taatttgtaa attttaataa taaaacacat ctaattccta tattttcatt aaatattaac
     1681 caaaataaaa ataaataata catatttaac atgatgtctt ttttcgcaaa tcaagcacca
     1741 ataagcctaa atgcgagaaa atggcagtaa tcatgaatgt acaaaaaaca ccaaagggaa
     1801 attgcagaaa gtataaagac gtactacgaa tttttacttt gcttgcctgc ctcgccggca
     1861 gacaggccga tagaaaaaat atcatacggc ctaatggcta cctgatattt caatttggga
     1921 ttaatcggct gtaattcttc cggtaagttt caaaagaccg tcgatgatag ttagcggatg
     1981 gggcggacaa cctgggatgt aaaggtcggg ggtatttttt tcaaaaaagc cacggttcag
     2041 cgctttactg tttttaaaca ctcctccgct gatagcgcag gttcccacgg caacaagtat
     2101 tttgggatca ggtacagcct cccaggtaag ttcgagcgcc tttgcactat tttcaaccaa
     2161 tggccccgtt accacaatgc catcggcatg acgcggcgaa gccacaaact caataccata
     2221 acggctgaca tcaaaattca cattgtcgga tgcattcagt tcaagttcgc aaccgttaca
     2281 actgccatcg gaaaccagac gcagttttaa agacctgccg aataccttgg tgatttcttt
     2341 gcgaactaat tccggatcaa atataatatg gtccgtattg gatgtaacag tcaggcgcga
     2401 actattattg gtggcgatac ggaagttgtt agaaaacctg attatgtctt ctttacaact
     2461 tacacattcg ccacaaaaga cacattgtcc aaggttaagg gtcagcggat ttttggatat
     2521 agcctctgca gggcataatt ccacacaagc gtta
//
LOCUS       DAOPZK010000218         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_58557_length_2533_cov_24.219532, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000218 DAOPZK010000000
VERSION     DAOPZK010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58557_length_2533_cov_24.219532"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1211
                     /locus_tag="PKH68_09105"
     CDS             177..1211
                     /locus_tag="PKH68_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13131.1"
                     /translation="MKKNILLTFDYELFLGSRSGTVENCLIRPTEKILNILKENQARA
                     IFFIDTTYLYRLEKLSETNANAERDFNIIKQQLVEIASEGHYLFHHLHPHWLDARYLE
                     DINQWDLSDTGRYAFASLNESGKDMLFKYSDSFLTDINAKAHSKNKCNGYRAGGLYIE
                     PFDSFKPFFEKYGIHYEFSVVPGEKKTGEKLFYDFTQCPADRPYSFSENPTVESVQGG
                     FIEFPITKIKIKGITKLLNSIYYRSSKITPGNKLFGDGVSVTSAIRNSDNDGSGKKYL
                     SFEMAISIELLNPVLLALFKRTIKQKHFVHFLSHPKLQSEVSVRSMDKLLQFCNSNFE
                     CEFDFMGFYK"
     gene            1211..2515
                     /locus_tag="PKH68_09110"
     CDS             1211..2515
                     /locus_tag="PKH68_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13132.1"
                     /translation="MKKILILSYFFPPSTFTGSFRIYSWAKYLSRFGWYPIVVTRSWD
                     VPVNNYQDMSATTSGKIVHQKFENYEVYFLPYKGNLRDRLYARYGDRRMVVLRKILSF
                     FEQIVQNYGLWVVPFKNLYKFSGKLLEENKDIKCMITSGRPYVLFHFCHKLTKKYNIP
                     WIADYRDDWNTSQWLRNIPFKDKVIMKFESRSEKKWLSNAACFTTISQAYVNSISGFV
                     KKSGYAVMNGFDPEDYRDIVPAENSNVFTILFNGSLYDTQPVEIFTNAYRKFVQSPDK
                     KEKIKLVFLGLNFEKLQSDRVRKLLEGLESFYEITDRFEKKKALGIMAASHLFLMFSH
                     TNIKGVTSSKIFDYLALGKPILLCPSDNEILEEIVVSTNSGFVCDTADNIISVLNQLY
                     DKYVLTGDTSCTRENNTVEFYSRINQTKKMAEIINTATSAGG"
BASE COUNT          820 a          476 c          505 g          732 t
ORIGIN      
        1 caaagcatta caaagtgcac acaaagtctg ccacggcaga cactcagact aattatacac
       61 aatattgcct tttaagacag cctccaatat caaattagaa tgtcattcca tgtagcacag
      121 caatataatg aatctttatt taattttgtt gcttctaaaa agatttatcc gatacaatga
      181 aaaagaacat tcttcttaca ttcgattatg aattgtttct cggcagccgc agcggaacgg
      241 ttgagaactg cttaattagg ccgactgaaa aaattctgaa tatattgaaa gaaaatcagg
      301 cacgggcgat tttttttatt gataccacat atttgtaccg actcgaaaag ttaagtgaaa
      361 ccaatgcgaa tgcggaacgt gattttaaca taataaagca acagttggta gaaatagcct
      421 ctgaaggcca ctatctgttc catcatctgc atccgcactg gctggatgca cggtatcttg
      481 aagatataaa tcaatgggat ttgtcggata ccggacgcta tgcctttgcc tcactgaatg
      541 aatccggcaa agatatgctg tttaaatact ctgatagttt cctgacagat attaatgcta
      601 aggcacattc aaaaaataaa tgtaatggat acagggcagg tggactgtac attgaaccgt
      661 ttgacagctt caaacctttt tttgagaagt atggaataca ctatgagttc agtgtggttc
      721 cgggagaaaa aaagacaggt gaaaaattgt tttatgattt tacacaatgc cctgccgaca
      781 ggccttattc gtttagtgaa aatcccacgg tagaatctgt tcagggagga tttattgagt
      841 ttccgataac aaaaattaaa attaagggta ttaccaaatt attgaactca atttactaca
      901 gatcaagcaa aataacgccg ggaaacaagc tgtttgggga tggcgtgtcc gtgacttccg
      961 cgatcagaaa ctcggacaat gatgggtcag gaaaaaaata cttatcgttt gaaatggcca
     1021 tcagcattga attgctcaat cctgtcttac ttgctttatt caaaaggact atcaaacaaa
     1081 aacactttgt gcatttttta tctcatccca aactgcagtc tgaagtaagt gtgcggtcaa
     1141 tggataaatt gctgcaattt tgtaatagta attttgaatg tgaattcgat tttatgggat
     1201 tttacaaata atgaaaaaaa tactgatcct atcatatttc tttccaccct ccacgttcac
     1261 ggggagtttc agaatctact cctgggccaa atacctgtcg cgctttggct ggtatcccat
     1321 cgtggttacc cgcagctggg atgttcccgt gaacaattac caagacatgt cggccacgac
     1381 ctccggcaag atcgtccatc agaaatttga aaactatgag gtctactttc tcccatacaa
     1441 aggcaacctc agagaccgcc tgtatgctcg gtatggcgac cgacggatgg tcgtactgag
     1501 aaaaattctc tctttttttg agcagatagt acaaaattat ggtttgtggg tggttccgtt
     1561 caaaaatctg tacaaattct ctgggaaact actggaagaa aacaaggata tcaaatgtat
     1621 gataacatca ggacgccctt atgtcttgtt tcacttttgc cataagttaa cgaagaagta
     1681 taatatacca tggatagccg actatcgtga tgactggaat accagccagt ggctcagaaa
     1741 tattccgttt aaagataagg ttattatgaa atttgaaagc agaagtgaaa aaaaatggct
     1801 ttcaaatgca gcatgtttta cgaccatatc gcaggcttat gtaaattcaa tttccggttt
     1861 cgtgaaaaag agcgggtacg ctgttatgaa cggatttgat cctgaagatt acagggatat
     1921 tgttcctgct gaaaacagca atgtatttac catactgttc aatggctcgc tttatgatac
     1981 acagccggta gagatcttca ccaatgcgta tagaaaattc gttcagtcgc ccgacaaaaa
     2041 agaaaaaata aaattagttt tccttggttt gaattttgaa aaattacagt ccgaccgggt
     2101 aagaaaactc ctggaaggcc tggaatcatt ttatgaaatc accgaccggt ttgaaaaaaa
     2161 gaaagcactc ggaataatgg ctgcatccca tttgtttctg atgttctcgc ataccaatat
     2221 taagggtgtg acatcgagca aaatatttga ttaccttgct ttgggaaagc cgatattatt
     2281 gtgcccatcc gataatgaaa tacttgagga aatagttgtc tcaacaaatt cgggattcgt
     2341 ctgcgatact gcggataata tcatttcggt actgaatcaa ctttacgata aatatgtcct
     2401 gaccggtgat acttcctgta caagagaaaa caatactgtt gagttttatt cacgaatcaa
     2461 tcagacaaaa aaaatggctg aaataattaa tacagcaaca tcggcaggag gttaatcatt
     2521 cagacttgtt tac
//
LOCUS       DAOPZK010000219         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_58954_length_2523_cov_33.091977, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000219 DAOPZK010000000
VERSION     DAOPZK010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58954_length_2523_cov_33.091977"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKH68_09115"
     CDS             complement(<1..242)
                     /locus_tag="PKH68_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842471.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HNX13133.1"
                     /translation="MNQEILKLKPKSVWGYFDEIMQIPRPSGKEQKIIAYLKAFGQKH
                     KLETLVDKVGNVLIRKPATKGREKLPWIVLQSHVDMV"
     gene            complement(398..1612)
                     /locus_tag="PKH68_09120"
     CDS             complement(398..1612)
                     /locus_tag="PKH68_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNX13134.1"
                     /translation="MKDNKILNITTDVKWIGVLDHDIRTFDIVMETKYGATYNSYFIN
                     AQKKTIVEATKEKFWDVYLEKLKQVVNPAEIEYIVMNHTEPDHSGNAINLLKIAPRAK
                     IVGTGNVIRYMTDLMNMDFPHIIVKDGDTLDLGNKTLRLINAPNLHWPDSMYSYLEED
                     KVLFTCDSFGSHYCHEEMYDDKVGNFDDAFKYYFDVILKPFSKFMLKAIEKIRPLDIS
                     AICTGHGPLLTKNWKRYVDLTEQYAKEAVAFPEFGRVLVAYVSAYGKTKILAESIAKG
                     LSEVEGIHADLCDIEKMPAGELADKITRATGIIIGSCTINQNSLPQIYTMFALMSPLR
                     DKGKPAGCFGSYGWSGEAQKIIEANLSALKLTLVPDNIFIKFTPSADDISRCVEYGKS
                     FAAKVLDIRCGG"
     gene            complement(1633..>2523)
                     /locus_tag="PKH68_09125"
     CDS             complement(1633..>2523)
                     /locus_tag="PKH68_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13135.1"
                     /translation="WFDESVSRLNTEQRGRFLGSFAPEDKIITFMKSGHFRIYNNDLS
                     IHFDEDLLLIEKYNPEKVYTVIYYDGESGFTFVKRFTAEFSDKKQIVISENTGSHMIE
                     FMPEKNPAVELTYEMKNARSKPSETIDLVEFIGVKSYKAKGKRLSNNQILTITRIITD
                     EPPEEPQAARSQDEISEALPPDFTGGEEDDSGIEQSSDTTTGVEDKVDNIPPEIIDNA
                     EDKVEKNLEQKTGANPAENKSKTAKTLIEKASREEDTLSEKPEKPAAEEILPARQKKG
                     TKKPKGKNDFPGAIQIEFDF"
BASE COUNT          631 a          507 c          555 g          830 t
ORIGIN      
        1 accatatcca catggctttg caagactatc cagggaagtt tctctctgcc cttggtggcc
       61 ggtttgcgga taagcacatt gcccacttta tcgacaaggg tttccagttt atgtttctgt
      121 ccgaatgctt tcaggtaagc aattattttt tgctcttttc ccgaaggccg aggtatctgc
      181 attatttcat cgaaatagcc ccacacagat ttgggtttta gttttaaaat ttcctgattc
      241 atatttttta agttaaaagt tgaaagttgc aagttacaag ttacaagtag aaagtagaaa
      301 gtagaaagta gaaagtggaa agtggaaagt ggaaagtata tagagtttta attaattgaa
      361 aaacaggtca caatttactg ataaatggcc gttgttgtta tcctccgcac cttatatcca
      421 ggactttggc ggcaaagctt ttgccatatt ccacacagcg tgagatatca tcggctgaag
      481 gggtaaattt aataaaaata ttgtccggaa cgagggtaag tttcagcgct gagaggttgg
      541 cttcaataat cttttgcgct tcgccgctcc atccgtacga accgaagcat ccggctggtt
      601 tgcctttgtc gcgcagtgga ctcatgaggg caaacatggt gtatatttgt ggcagggaat
      661 tttggttgat ggtgcaacta cctataatga tgccggtggc acgtgtaatt ttgtcggcca
      721 gttcgccggc aggcattttt tcgatatcac acaggtcggc atggatgcct tcgacctccg
      781 agaggccttt ggcgatgctt tcggcaagaa tttttgtttt tccataagca gaaacataag
      841 ctaccagcac cctgccaaat tcaggaaaag ccactgcttc tttagcatat tgttcggtta
      901 aatccacgta acgtttccag ttttttgtga gcaaaggccc gtgtccggta caaatagccg
      961 agatatcgag cggcctgatt ttttcgatag ctttcagcat aaacttactg aaaggtttca
     1021 ggatgacatc aaaataatat ttgaaggcgt catcaaaatt gccgacttta tcatcgtaca
     1081 tttcttcgtg gcagtaatgt gagccgaatg aatcgcaggt aaataaaact ttatcttctt
     1141 caagatagga atacatagaa tcgggccagt gcaggttggg ggcattgatt aaccgcaggg
     1201 ttttgttgcc aaggtcgagg gtatcgccgt cttttacgat gatgtgcgga aaatccatat
     1261 tcatcaggtc ggtcatatac cggataacat ttcccgtacc gacaattttt gcacggggtg
     1321 caattttcag caggtttatg gcattgcccg agtggtcggg ttccgtatga ttcattacga
     1381 tatattcgat ttccgcggga ttgactactt gtttaagttt ctcaagatat acatcccaaa
     1441 atttttcttt ggtagcttca acaatagtct ttttttgtgc attaatgaaa taagagttgt
     1501 aggtagcacc gtatttggtt tccatcacga tgtcgaaagt gcgtatatcg tggtccagca
     1561 cccctatcca ttttacgtcg gtggtaatgt ttagtatttt gttatccttc ataaaaagta
     1621 taattattaa ggttaaaaat caaattctat ctgtatggca cccggaaaat catttttccc
     1681 tttaggtttt tttgttcctt ttttctgtct ggcaggaagg atttcttcag cagcaggctt
     1741 ttcgggtttt tcagacaagg tatcttcctc cctgcttgcc ttttcaatta aagttttggc
     1801 tgttttggat ttattctcag cgggattagc gcctgtcttt tgttccagat ttttttcaac
     1861 tttatcttca gcgttgtcaa tgatttcagg aggtatattg tcaactttgt cctctacccc
     1921 tgtggtagta tcgctgcttt gctcaatacc tgaatcatct tcctctccgc cggtgaaatc
     1981 cggcggcaag gcttctgaaa tttcatcctg tgatctggca gcctgaggtt cttcaggagg
     2041 ttcatcggtg atgatacgcg taatagtgag tatttggttg ttcgacaggc gttttccctt
     2101 cgctttgtaa ctcttaacgc cgataaactc gaccaggtcg atagtttcag aaggtttgct
     2161 acgggcattt ttcatttcgt aggtgagttc aacggcagga tttttctccg gcataaattc
     2221 tatcatgtga gaaccagtat tttcgctgat gacaatttgc tttttatctg aaaactcggc
     2281 cgtgaaccgt tttacgaaag taaagcccga ctcgccgtca taataaatta ccgtgtacac
     2341 cttttcggga ttgtatttct caattaaaag aaggtcctca tcaaaatgta ttgacaaatc
     2401 gttgttataa atcctgaaat gccctgattt catgaaggta attattttgt cttcgggagc
     2461 gaatgatccc aggaatcttc cacgttgctc ggtattcagg cggcttacgc tttcgtcgaa
     2521 cca
//
LOCUS       DAOPZK010000220         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_60357_length_2484_cov_22.501853, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000220 DAOPZK010000000
VERSION     DAOPZK010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60357_length_2484_cov_22.501853"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..943
                     /locus_tag="PKH68_09130"
     CDS             2..943
                     /locus_tag="PKH68_09130"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNX13136.1"
                     /translation="MPWSNCVATVGFFDGVHAGHRFLLHQLSKIARDEHKISVVITFD
                     NHPRKVLNADFQPKLLTSLDEKLDLLTEAGLDACVVLSFTKELSRLSAFEFLQQILFD
                     RLKVSTLLVGHDHRFGHNREQGFDEYRAFGATLGMNVIQVEKYTTTETPHMSSTQIRH
                     SLESGDLAQANKLLTYPFILTGKVKSGFQVGRKIGFPTANLLPAEKDKIIPATGVYAV
                     RVKWDHHEYRGMMNIGYRPTINNSDERTLEVHIIDFKGDIYDRNLKIEFIAKIRDEMK
                     FNGVDELIEQLKKDRETVLNMDFNKIVENFTESDLFK"
     gene            1004..>2484
                     /locus_tag="PKH68_09135"
     CDS             1004..>2484
                     /locus_tag="PKH68_09135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNX13137.1"
                     /translation="MKKLFFALMIGTIMVACTAKTDNPFFQSYENEFGAPPFDKIKTE
                     HYMPAFTEGMKQHQAEIDSIANNPEAPTFANTIEKMDFSGELLKKVSSAFFNVQSADT
                     NDDLDSIAKEITPELTAHNDNIYLNDKLFQRVKTLYDNRTNLGLTAEQNRLLEKYYKN
                     FIRSGAALNETQKAGLREINKELSSAELNFAQNVLAETNAYQKFVTSKDELKGLPEGV
                     IQAAAEGAKEAGKEGQWLFTTQKSSFIPVLQYGENRELRKELLMAYTTRCNHDNANDN
                     KKLINTIMKLRVKKAQLLGFESPAAFILDNTMAKTPEAVYSFLQTVWTPAVAKAKVEA
                     AELQKMMDAEKKGEKLEAWDWWYYTEKLRKEKFDLNEEELKPYFKLENVREGVFGLGG
                     KLFGLKFEKITGMPVYNKDVETFKVLDADGSLIGILYTDYFPRAGKASGAWMNNISEQ
                     YIKDGVNHRPIIVNVGNFTKPTSDKPSLLTMDEVETMFHEFGHA"
BASE COUNT          808 a          539 c          543 g          594 t
ORIGIN      
        1 aattccctgg agcaactgcg tagctacagt cggtttcttc gacggagtgc atgccggaca
       61 ccggtttttg cttcatcaac tgagtaaaat agcccgtgat gaacataaaa tttcggtggt
      121 cattacattc gataatcatc cccgcaaggt gctgaatgcc gatttccagc ctaagctcct
      181 gacatcatta gatgaaaaac tcgatttgct taccgaagct ggtctggatg cctgcgtagt
      241 tctcagtttc actaaggaat tatcccggct ttcggccttt gaatttttac aacaaatttt
      301 gtttgatcga ttgaaagtga gtaccttgct ggtggggcac gatcaccggt ttgggcataa
      361 ccgcgagcag ggtttcgatg aataccgcgc ttttggagca acactgggca tgaatgtgat
      421 tcaggtggaa aaatacacaa ccactgaaac tcctcacatg agcagtacac aaatcaggca
      481 ttcgctggag agcggcgatc tggcacaggc caataaattg cttacttatc cgtttatatt
      541 gacgggaaaa gtaaaaagcg gctttcaggt gggaagaaaa atcggatttc ccaccgccaa
      601 tctgctgcct gccgaaaaag ataaaataat acctgccacc ggtgtgtatg ctgtgcgtgt
      661 gaaatgggat catcatgagt accgcggtat gatgaacatc ggataccggc ccaccatcaa
      721 taattccgat gagcgtacac tcgaagtgca tattattgat tttaagggtg atatatacga
      781 tagaaacctg aaaatagaat ttattgcaaa aattcgcgac gaaatgaaat tcaacggtgt
      841 ggacgaatta atcgaacaat tgaaaaaaga cagagaaacg gtgctgaata tggactttaa
      901 caaaattgtt gaaaacttta cagaatctga tttgtttaaa tagtcagttt ttaagaatat
      961 ctgtatcttt acagcataaa aataaattat aaataacata acaatgaaaa aactattttt
     1021 tgcacttatg attggaacaa taatggtggc ttgtactgcc aaaacagaca atccattctt
     1081 ccaaagttat gaaaatgagt ttggcgcacc gccattcgac aaaatcaaaa cggagcatta
     1141 catgcccgca tttaccgagg gaatgaaaca gcatcaggcc gaaattgaca gcatcgccaa
     1201 caaccctgaa gcacccacat tcgccaatac catcgaaaaa atggatttca gcggtgaatt
     1261 gttgaaaaaa gtctcttctg catttttcaa tgtacagtcg gccgatacga atgacgatct
     1321 ggattccatt gccaaggaaa ttacaccgga actgaccgcg cacaacgata atatctattt
     1381 gaacgataag ctttttcaac gcgttaaaac actctatgat aaccgcacaa acctgggact
     1441 gacagccgag caaaaccgat tactggagaa gtattacaaa aactttattc gcagcggtgc
     1501 agcgctcaac gaaactcaga aagccgggct gcgcgaaatc aacaaggaac tgagttccgc
     1561 cgaactgaac tttgcacaga atgtgctggc cgaaaccaat gcatatcaaa aatttgtaac
     1621 cagcaaggat gaactgaaag gattgcccga aggagttatt caggctgctg ccgaaggagc
     1681 caaagaagcc ggaaaagaag gtcagtggct gttcaccact caaaaatcaa gctttatacc
     1741 cgtgcttcaa tacggcgaaa accgtgaatt gcgcaaggaa ttactgatgg cgtacaccac
     1801 ccgctgcaat cacgacaatg ccaacgataa caagaaactc atcaatacca tcatgaaact
     1861 gagggtgaaa aaagcacagt tactgggctt tgaatcaccg gcagctttta tcctcgacaa
     1921 taccatggcc aaaacacccg aagctgttta cagtttcctc caaacagtgt ggacgcctgc
     1981 tgtagccaaa gccaaagtgg aagcggccga actgcagaaa atgatggatg ccgaaaagaa
     2041 aggcgagaaa ctggaagcat gggactggtg gtattacacc gaaaaacttc gcaaggaaaa
     2101 atttgacctc aacgaagaag aactgaaacc ttacttcaaa cttgaaaatg tgcgcgaagg
     2161 tgttttcgga cttggaggca aactttttgg tctgaaattt gaaaaaatta ccggtatgcc
     2221 ggtttacaac aaggatgtgg aaacattcaa agtactcgat gccgacggtt cgttgatcgg
     2281 tatactttac accgactatt tcccccgtgc cggaaaagct tcgggagcct ggatgaacaa
     2341 catcagcgaa caatacatca aagacggcgt gaatcatcgt cccattattg tcaatgttgg
     2401 taatttcacc aaaccgactt ccgacaaacc ttctttgtta accatggacg aagtggaaac
     2461 aatgttccac gagtttggtc atgc
//
LOCUS       DAOPZK010000221         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_61217_length_2461_cov_18.324190, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000221 DAOPZK010000000
VERSION     DAOPZK010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61217_length_2461_cov_18.324190"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1557
                     /gene="hflX"
                     /locus_tag="PKH68_09140"
     CDS             298..1557
                     /gene="hflX"
                     /locus_tag="PKH68_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNX13138.1"
                     /translation="MTEKDTIKTEIYQETAVLVGLITPTQSEVKAREYLDELAFLAET
                     AGAIPEKTFFQRLDYPNPKTFVGPGKLQEIKAYVVEHNIGIVIFDDELSPKQIRNIEN
                     ELVDKDPNATDLVKILDRTNLILDIFAKRAQTANAKTQVELAQYQYLLPRLTGLWTHL
                     ERQRGGGIGRYGPGETQLETDRRIILTKITLLKENLKTIDRQMATQRKNRGKMVRVSL
                     VGYTNVGKSTLMNLLSKSDIFAENKLFATLDTTVRKVIIDNLPFLLSDTVGFIRKLPT
                     NLIESFKSTLDEVREADLLLHVVDISHPNFEEQLEVVNQTLKEIDKTEKPVILIFNKI
                     DAFTYTPKDEDDLSPVRKENISLDELKKTWMSKLHDNCIFISAREKTNIDELKELLYK
                     KVKQIHVERYPYNDFLFEKYDNDEFEE"
     gene            1744..>2461
                     /locus_tag="PKH68_09145"
     CDS             1744..>2461
                     /locus_tag="PKH68_09145"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNX13139.1"
                     /translation="MENYRNLTEKEVATLVVYGCSADNWSNVTVDPEFTPQYVTNVHF
                     SGTIKLGKFDKVFEMDGGFRKHSGLYNCCLHNCTIGDDVFIENIHNYMANYDVGNGTY
                     IENTNLIVVDGKTTFGNGVRVPVMNETGGREVPIYDFLSAPLAYILTLYRHRPQMIEN
                     LQKMIDDYSESISSDRGKIGENVEIINCGRIKNVRIGDSSSIKGVSNLKNGSINSTTK
                     APVHIGSGVKCTDFIICSGVS"
BASE COUNT          796 a          552 c          529 g          584 t
ORIGIN      
        1 cggtaaaaat atttagttcc atcgcttcgc tctggatgac aggaaatgat agtgatttat
       61 ggagggagtt ggttagcggc gaagccgcca accaactccc tccataaaga taaaatactg
      121 actgtcattt tgaacgaaat gagaattgaa aatcggcgaa gccaaatctg atatatttca
      181 agaatttaga attttaccgt acaactgtta ttctttttat agaaaatgac aactccccca
      241 attcataatt tcaatattta tcgtactttt gtacttacag ataattgaaa cccgacgatg
      301 accgaaaaag acactataaa aactgaaata tatcaggaga ccgccgtact ggtgggtctc
      361 atcactccta cccaatcgga agtgaaagcc cgggaatacc tcgacgagct ggcatttctg
      421 gccgaaacag cgggtgcgat acccgaaaag acatttttcc aacggttgga ttaccctaat
      481 ccgaaaacat ttgtggggcc gggcaagctg caggaaataa aagcctatgt ggtagaacat
      541 aatattggta ttgtcatctt tgacgacgaa ctttcgccca aacaaatccg gaacattgaa
      601 aatgaactgg ttgacaaaga tccgaacgcc accgacctgg tgaaaattct ggaccgtacc
      661 aaccttatac tggatatatt tgccaaacgg gcgcaaactg ccaatgctaa aactcaggta
      721 gagctggcgc aataccagta cctgttgcca cgcctcaccg gattgtggac tcacctcgaa
      781 cgtcagcgtg gaggtggtat cggacgctac ggaccgggtg aaacacagtt ggaaaccgac
      841 cgtcgaatta ttctcactaa aattacattg ctcaaagaaa atctgaagac cattgacaga
      901 caaatggcca cccaacgtaa aaatcgcggg aagatggttc gtgtgtccct ggtgggttat
      961 accaatgttg gtaaatcaac cctgatgaac ctgctgagca agtctgatat ttttgcggaa
     1021 aacaaactct ttgccacact ggacactacg gtgcggaagg taattatcga caatctgcct
     1081 ttcctgctgt ccgatacggt gggttttatc cgcaagttgc ccaccaacct gatagaatcg
     1141 ttcaaatcga ccctcgacga ggtgcgcgaa gccgacctgc tgctgcacgt ggtggatatc
     1201 tcccacccca actttgagga gcaactggaa gtggtaaacc aaaccctgaa ggaaatagat
     1261 aaaaccgaga aaccggtaat actcattttc aacaaaatag acgcgtttac ctacacaccc
     1321 aaagacgagg acgatctcag cccggtaagg aaggaaaata tcagcctgga tgaactgaaa
     1381 aaaacctgga tgtcgaaact gcacgacaac tgcatcttca tttcagccag ggaaaaaaca
     1441 aatatcgatg aattgaagga actgctttac aaaaaagtga aacagattca cgtggagcgg
     1501 tatccttaca acgattttct gttcgaaaaa tacgacaacg acgaatttga ggaataattt
     1561 taattccaat aaataaaaaa ctcagcccga cacatcgcaa ccagataacc cggtgagatg
     1621 tgtcggtgct aaaaaaatca ggcatcaaca gcttgccatt cccgctctgc cttatatcag
     1681 tactgaaaaa tcacccgatt aaattcattc aattctgaac tctatttttt caaaaaatca
     1741 tttatggaaa attacagaaa tcttaccgaa aaggaagttg ccacacttgt agtgtatggt
     1801 tgcagtgccg ataactggag caacgtaaca gtcgacccgg aatttacacc acaatatgta
     1861 accaacgtgc atttttcggg aacaatcaaa ctggggaaat tcgacaaagt cttcgaaatg
     1921 gacggtggat tccgtaaaca cagcggattg tacaactgct gcctgcataa ttgcaccatt
     1981 ggcgacgatg tgtttataga gaacatacac aattatatgg ccaactacga tgtgggtaac
     2041 ggcacctaca tagaaaacac caacctgata gtggtggatg gaaaaactac ctttggtaat
     2101 ggcgttcgtg ttccggtgat gaacgaaacc ggtggccgcg aagtacccat ctacgatttt
     2161 ctgtcggctc ccctggccta catactcaca ctgtaccggc atcgcccgca gatgattgaa
     2221 aatctgcaga aaatgataga cgactactcc gaaagtataa gttctgaccg gggaaaaatt
     2281 ggcgaaaatg tagaaatcat caattgcggt cgcatcaaaa atgtacggat tggagacagc
     2341 agttctatta aaggggtatc aaatctgaaa aacggttcaa tcaatagcac aaccaaagcg
     2401 ccggttcata tcgggtcggg tgttaaatgc accgatttca ttatttgctc cggagtatcc
     2461 a
//
LOCUS       DAOPZK010000222         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_61379_length_2457_cov_25.287261, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000222 DAOPZK010000000
VERSION     DAOPZK010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61379_length_2457_cov_25.287261"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKH68_09150"
     CDS             <1..635
                     /locus_tag="PKH68_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNX13140.1"
                     /translation="TAQLLFEKVKERKRDIRAYISASATGYYGMVTSGKIFHESDSPS
                     SDFTGVTCREWEEAGKMFQNAGIRTVFIRTGVVLAKESEALSKMALQVRMGFGSATGS
                     GKQYFSWIHIDDLCRMYISAIENETIQGAYNATAPEYITNNGFMRKLSLVLRKPFWFP
                     RIPALAMKMLFGEMSGLLLRGSRISSEKIRSTGFEFLYPDVESALKNILK"
     gene            665..1897
                     /locus_tag="PKH68_09155"
     CDS             665..1897
                     /locus_tag="PKH68_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HNX13141.1"
                     /translation="MTSAGAFLPAFSENTTLKTVSKRLQVPAAVSDREYLWKLLYKIS
                     EPVLRNMSNGELRKNMTVELSPTWDGRDKNVTYMECFGRLMAGLAPWLTLPDDNTEEG
                     LKRSQLKDWALKSYTHSVNPQSPDYLLWRKEGQPLVDSAYFSNALMRAPRQLWDPLDG
                     VTKSRIIAELKQLRRISPPYTNWLLFAAMNEAFLLSIGEEYDRMRIDLTLKKLNEWYV
                     GDGWYADGEKFHFDYYNSFVMHPMLLEVLEVLVKAGKADKSHYEQALKRTQRFAEHLE
                     RLISPEGTYPPFGRSLTYRTGAFQPLALLAWRKQLPAKLTEGQVRAALTAVHKRIFDN
                     PTNFTPDGYLTIGFAGHQPELGDWYSNNGSMYITSESFLPLGLPAGDTFWTSKEEAWT
                     AKKAFINERFNKDYPVEY"
     gene            1923..>2457
                     /locus_tag="PKH68_09160"
     CDS             1923..>2457
                     /locus_tag="PKH68_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HNX13142.1"
                     /translation="MTRRKILFLFSILLFFPLNAQKLIDTGGKAYSRTSVNTTVFRSN
                     SVVSSGQYQYVAFYDEDCNLVLGQRKLSGKKWTLAKTQYKGNCADAHNVISIMADDDG
                     YLHLSFDHHGNSLNYCRSLLPHSLEMGEKQAMTGADENNVTYPEFYRLKNGNLLFVYR
                     SGASGRGNMVMKTYNLKS"
BASE COUNT          707 a          542 c          574 g          634 t
ORIGIN      
        1 tgacagctca attacttttt gaaaaagtaa aagaaagaaa acgtgatatc cgggcgtata
       61 tatctgcatc tgccacaggt tattatggta tggttacttc cggtaagata tttcatgaat
      121 cagattcccc gtcatccgat tttaccggag tgacgtgccg cgaatgggag gaagcgggca
      181 agatgtttca gaatgcaggt atcagaactg tgtttatccg aaccggtgtg gtgcttgcca
      241 aagaaagtga agccttatcc aagatggctc ttcaggttcg gatgggattc gggtcggcca
      301 ccggcagtgg aaaacagtat ttttcctgga tacacatcga tgatttgtgc cggatgtaca
      361 tcagtgcaat agaaaatgaa acaatccaag gagcttacaa tgccacagct cccgaatata
      421 ttaccaataa cgggtttatg cgtaagctgt cactcgtgct tcgaaaacca ttttggtttc
      481 cccgaatacc agcgttggcc atgaaaatgc tgttcggtga aatgtccggg ctgttactcc
      541 ggggaagccg gatttcttcc gaaaaaataa gaagtaccgg ctttgaattt ctgtatccgg
      601 atgtggaaag tgctcttaaa aatatactta aatgatacga cgaaatttct tgaaaatatt
      661 gggaatgact tctgccgggg cttttctgcc tgctttttcc gaaaatacta cccttaagac
      721 tgtctccaaa cggcttcagg tacctgctgc cgtttcagac cgggaatatc tctggaaatt
      781 actgtataaa atttcagaac cggtgctacg gaatatgtcc aacggcgaat tgcgtaaaaa
      841 tatgactgta gaactgagcc ccacctggga cgggcgcgac aagaatgtga cgtatatgga
      901 atgtttcggt cggctgatgg ccggactggc accgtggctt accttgcccg acgacaatac
      961 ggaggaggga ttaaaacgct cgcaactgaa ggactgggcg ttgaaaagtt atacccattc
     1021 agtgaatccg caaagtcccg attatctgct gtggcgcaaa gaaggacagc cgttggtcga
     1081 ttcggcctat ttttccaatg cacttatgcg tgctccccgc caactgtggg acccgctcga
     1141 tggagtaaca aaaagccgga tcatagctga actcaaacaa cttcgccgca tttctccgcc
     1201 ttataccaac tggcttctgt ttgctgccat gaatgaagct ttcctgctat ccattggcga
     1261 ggaatatgac cggatgcgaa tagatctcac actgaaaaaa ctcaacgaat ggtacgttgg
     1321 agacggctgg tatgccgatg gggagaaatt tcattttgat tattacaatt catttgtaat
     1381 gcatcccatg cttctggaag tgctggaagt gctcgttaaa gcaggaaaag ccgacaaatc
     1441 gcactatgaa caggcattga aaagaacaca gcgatttgcc gagcatctgg aacggttgat
     1501 atcacccgaa ggaacctatc cgcctttcgg acggtcgctc acctatcgca cgggggcatt
     1561 tcagccactg gctttgctcg cctggcgaaa acagctgcct gccaaactga ccgaaggaca
     1621 agtgagagct gcattaacag cggtacacaa acgaattttc gacaatccaa ccaattttac
     1681 tcccgatgga tatcttacta tcggatttgc cggtcatcaa cccgaacttg gcgactggta
     1741 cagcaataac ggaagtatgt acatcacttc cgaaagtttt ctgcctcttg gtttgcccgc
     1801 cggtgacaca ttctggacct cgaaagagga agcctggacg gcaaaaaaag cattcataaa
     1861 tgaacgtttc aataaagatt atcccgtgga atattaactg taagtattcc ataatattga
     1921 atatgacaag acgcaaaatc ctttttttat tttcaatact cctttttttt ccgctcaatg
     1981 ctcagaaact aattgatacc ggaggaaagg catacagccg gacatcggta aatacgaccg
     2041 ttttccgttc caactcggtg gttagttccg gtcaatatca gtacgtggca ttctacgatg
     2101 aggattgtaa tcttgtattg ggacaaagaa aattatccgg gaaaaaatgg acgttggcca
     2161 aaacacaata caagggaaac tgcgctgatg ctcacaatgt aataagtatc atggccgatg
     2221 acgatggata cctgcatttg agctttgacc accatggaaa ttctctgaat tactgtcgca
     2281 gtcttttacc acattctctg gagatgggag aaaaacaagc tatgaccgga gcagacgaaa
     2341 acaatgttac ctatcctgaa ttctatcggt tgaaaaacgg caatctactc tttgtgtacc
     2401 gttcgggagc atcgggtaga ggcaatatgg tgatgaaaac gtataacctg aaatcaa
//
LOCUS       DAOPZK010000223         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_63902_length_2394_cov_36.980333, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000223 DAOPZK010000000
VERSION     DAOPZK010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63902_length_2394_cov_36.980333"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1847)
                     /locus_tag="PKH68_09165"
     CDS             complement(162..1847)
                     /locus_tag="PKH68_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNX13143.1"
                     /translation="MKTLFSKWIILAGILFFSINLTAQDKNFYIFLSFGQSNMEGAAR
                     IEPQYKSVDERFRMMAAVDCPDTDRKMGNWYTAVPPLVRCYTGLGPSDFFGRTLVEYL
                     PANIKVGIINVAIGGCKIELFDKDNYQTYTATAPFWMKGMIDAYDGNPYKRLVDMAKL
                     AQRDGVIKGILLHQGESNTGEVTWPSKVQKVYENILSDLNLKASEVPLLAGGVVPADQ
                     NGKCAVMNEIIAKLPETIPTAHFIPSDGCLQGGDSLHFSAKGYEMLGYRYASEMLSLM
                     GYKAVVPANFDKPRTGIAFGKIDTIRYKSKTVGAKRKALVYTPPGYTKKKKYPVLYLL
                     HGIGGDEKEWLRGGQPQVILDNLYADGKIQDMIVVMPNGRAMRDDRAVGDIMAPDKVK
                     AFADFEKDLLNDLIPFVEKKYSVLKNRENRAIAGLSMGGGQSLNFGLGNLDKFAWVGG
                     FSSAPNTKTPQELIPNPDEARKMLKLLWISCGDKDWLIPFSKRTHDYLVENNIEHVYR
                     VIPGGIHDFNVWKEGLYNYSQLLFKPVTPELIKKYQNIPPEGKPLGQPQFSRP"
     gene            complement(1865..>2394)
                     /locus_tag="PKH68_09170"
     CDS             complement(1865..>2394)
                     /locus_tag="PKH68_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNX13144.1"
                     /translation="LLENPDTKNTGVLNLGIGGNCVVRGGLGPTALERFDRDILSQSN
                     VRWLIILEGINDIGSAKTQVEADKIAAELIAAYDEMINKAHAKGILVYGATMLPFAGS
                     FYDTPYRQTARDAVNEWIRNSRRFDAVIDFDKIMRNPENVKTILTDMHDGDFLHPNQA
                     GYRRMGENVNLGLFK"
BASE COUNT          609 a          562 c          508 g          715 t
ORIGIN      
        1 aagggggaat ttagagattt aaattagatt tgaacgaata gtttagttag tttcaaacat
       61 tcccttttgt gtcaaaagta ttttggcatg actaattcat gtgtaaatag atgttttttt
      121 gctcacataa cttgtccctg attttgggga taatttacat gtcacggcct gctgaattgg
      181 ggctgtccaa gaggttttcc ttcaggagga atgttttgat atttctttat caattcgggc
      241 gtaaccggtt tgaaaagcag ttgagaatag ttatagagcc cttctttcca aacgttgaaa
      301 tcatggatac cgccgggtat taccctgtag acgtgctcaa tgttgttttc aaccaaataa
      361 tcgtgtgtac gcttgctgaa aggaataagc cagtccttgt cgccgcacga aatccagaga
      421 agtttcagca tttttctggc ttcgtcggga tttggaatga gttcctgagg ggtttttgtg
      481 ttaggtgcgg aggagaaacc acccacccat gcaaatttat caagattacc caatccaaaa
      541 ttcaacgact gcccgcctcc catggataat ccggcaattg cacgattttc acgatttttc
      601 aacacggaat attttttttc aacgaatggg atcagatcgt ttagcaaatc cttttcaaaa
      661 tcggcgaaag ccttcacctt atcgggcgcc atgatgtcac cgacggctct gtcatcacgc
      721 atggcacgtc cgttgggcat taccactatc atgtcctgaa tttttccgtc ggcatacaaa
      781 ttatccagaa taacctgcgg ctgaccgccc cgaagccact ctttttcgtc gccgcctatt
      841 ccgtggagca ggtaaagtac cggatatttc tttttcttcg tgtagcccgg aggtgtgtat
      901 accaatgctt tccgtttggc acccactgtt ttggatttat agcgaattgt atcaattttt
      961 ccaaaagcaa tccccgtgcg cggtttatca aaatttgcgg gaactactgc tttatagccc
     1021 atcagcgaga gcatttcaga agcataccga taacccagca tctcgtatcc ttttgcagaa
     1081 aaatggagac tgtcaccgcc ttgcagacat ccgtcagatg ggatgaaatg cgcagtaggg
     1141 attgtttccg gcagtttggc aattatttcg ttcatcacgg cacattttcc gttctggtcg
     1201 gcaggcacca caccacccgc cagcaatgga acttcggaag ccttcaggtt caggtcgctg
     1261 aggatatttt catagacttt ctgaactttt gaaggccagg ttacctcgcc ggtattggat
     1321 tcgccctgat ggagcaaaat tcccttgata actccgtcac gctgagcaag tttggccata
     1381 tccaccagcc gtttgtaggg atttccatcg taagcatcta tcattccctt catccagaag
     1441 ggagctgtag cggtataagt ctgataatta tccttgtcga acagttcaat tttgcatccg
     1501 ccaatggcga cattaattat tcccactttg atgtttgccg gaagatattc aaccagggtt
     1561 cttccgaaaa aatcagatgg tcccaggccg gtgtagcaac gaaccagcgg tggtacggct
     1621 gtgtaccagt tgcccatttt gcggtccgta tcgggacaat ctaccgctgc catcatccga
     1681 aatcgttcat ccaccgattt gtactgaggt tcaatacgcg cagcgccttc catgtttgat
     1741 tgtccgaaac ttaagaaaat atagaaattt ttgtcctgcg cagtcagatt aatactgaaa
     1801 aataaaattc ctgcaagaat aatccatttt gaaaaaagtg tcttcatttt tttattagca
     1861 taagttattt aaataaacct aaattcacat tttcacccat cctacggtaa ccggcctggt
     1921 ttgggtgcag aaaatcgccg tcgtgcatat ccgtcagtat ggtttttaca ttttcgggat
     1981 tgcgcatgat tttatcaaag tcaataaccg catcaaaccg tcgtgagttt cttatccatt
     2041 cgttgactgc atctctggca gtctggcggt aaggtgtatc gtaaaaagac ccggcaaagg
     2101 gcagcattgt agctccgtaa acgagaatac ctttggcgtg tgccttgttg atcatttcat
     2161 cgtaggcagc aatcaattcc gctgcaattt tatcagcctc cacctgtgtt ttagctgaac
     2221 caatgtcatt tattccttcc agaattatca accatcgaac gttgctttgc gataaaatat
     2281 ccctgtcgaa ccgctcgagt gcagtcgggc ccaaaccgcc ccggacaaca caattgccgc
     2341 caatgcccaa attcagcact ccggtgtttt ttgtgtccgg attttccaac aatc
//
LOCUS       DAOPZK010000224         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_65128_length_2366_cov_19.569450, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000224 DAOPZK010000000
VERSION     DAOPZK010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65128_length_2366_cov_19.569450"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKH68_09175"
     CDS             complement(<1..601)
                     /locus_tag="PKH68_09175"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNX13145.1"
                     /translation="MTLKKFILIFALFIGISGMIKAQNQYGTWTSVGLEKKTGNWNFG
                     AETEVRTIHYVRLINRWSLGLNADYKIAKSLKVGLGYEFMNVLDEKYLNYQFRNRFTA
                     DITGKQKLGNISFSLRERIQLTKKNDSKRIDMDGLIDSYNIDPAWVWRNKLDADYNIP
                     KCRFTPGISIETFYSLNDPDGNKLDKLRYILYTDYKINKH"
     gene            complement(601..1377)
                     /locus_tag="PKH68_09180"
     CDS             complement(601..1377)
                     /locus_tag="PKH68_09180"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNX13146.1"
                     /translation="MWTKFVKITPFKTLFILLLLLTLLPSGTQAQLQMAAAAEAEQAA
                     TVQAADETKESIDIFTTDTKVDKKGKDKIQLNFNFFFSLLIDLAFLALIISLIYYPNY
                     KRLDTVFTFVLFNVSIFLLTFLLNHIKISMGAAFGLFAVFSMLRYRTAGISVKDMTYL
                     FIFITMGLLSGIQLELYELLIIFGVLLVVLIVLDTRILLKREATKIIYFEDVELLHVD
                     RHPELIETLIKRTGLNIHRITVEEISYLRDFGMITIYYYE"
     gene            complement(1343..2104)
                     /locus_tag="PKH68_09185"
     CDS             complement(1343..2104)
                     /locus_tag="PKH68_09185"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNX13147.1"
                     /translation="MEKFLEILNELKPISLAEMDSVKLLNRTDVKYVFHKDELADILS
                     KAAEFYRTLTINERRFENYDSLYFDTEDYQLYSAHHNGRTNRFKLRMRTYMYSDIHFF
                     EIKFKTNGNRTVKKRIQLPEKTDIISGIALELLERRTDIPAETLKKAIRIKCKRITMV
                     DKLLSERVTIDFDLSYNIGDDVWHEWPELVIAEVKQEKSGHSPFIKIMFSKHIRQVSL
                     SKYCFGIANYVNTVRKNNFKTKIRYVDKICKNHTF"
BASE COUNT          667 a          437 c          488 g          774 t
ORIGIN      
        1 tgtgtttatt aatcttatag tccgtgtaca gaatatatct tagtttgtcc agtttattac
       61 catcgggatc gttgagagaa taaaaagttt caatagatat tccgggtgta aacctgcatt
      121 tgggtatgtt atagtcagca tccaatttat tacgccacac ccatgcagga tcaatattat
      181 aactgtcaat aagtccgtcc atatctattc ttttgctgtc gtttttcttt gtgagttgaa
      241 tacgttctcg cagggaaaaa gatatgttac ccagtttctg cttgccggtg atatcagcag
      301 tgaagcggtt acgaaactgg taattaaggt atttttcgtc cagtacattc atgaattcat
      361 agcccagacc aactttcagt gatttagcaa ttttgtaatc tgcattcagt ccgagactcc
      421 accggttgat gagcctgaca taatgtatag tgcgcacttc ggtctcagca ccgaaattcc
      481 agtttccggt ttttttctcc aatcccacac tagtccaggt gccgtactga ttctgcgcct
      541 ttatcattcc ggaaataccg atgaacaaag caaaaatcag aataaacttt ttaagtgtca
      601 ttattcgtag taataaatgg tgatcattcc gaagtcacgc agataggaga tttcttccac
      661 cgtaatacga tgaatattca atccggtacg tttgatgagt gtttctatca gttcgggatg
      721 acgatcaacg tggagcaatt ccacatcctc gaaatagatt attttggtgg cttcgcgctt
      781 gagcaaaatc cgggtatcga gcacaattag tacaacgagc agaacgccga aaattatgag
      841 cagctcgtac agttcgagct ggatgccgct gagcagtccc atggtgataa aaatgaacag
      901 ataggtcatg tccttgacac tgatgccggc cgtgcggtat cggagcatgg agaaaactgc
      961 aaataaaccg aatgcagcac ccatcgaaat ttttatgtgg ttgagcagga aggtgagcag
     1021 aaagatggag acattgaaca gaacaaaggt gaaaaccgta tccagtcttt tgtagttggg
     1081 ataatatatg aggctgataa tcagagccag aaatgccaga tcaatgagca ggctgaaaaa
     1141 gaaattgaaa ttcaattgaa ttttgtcttt ccctttttta tccactttgg tgtcggtggt
     1201 gaaaatgtcg atggattctt tggtttcatc ggcagcctga actgtagcag cttgttcggc
     1261 ttcggcagct gctgccatct gcaactgagc ctgagtaccc gatggtagta gtgttaatag
     1321 taacagtagg atgaataatg tcttaaaagg tgtgattttt acaaattttg tccacatagc
     1381 gtatttttgt tttaaagtta tttttgcgta cggtatttac atagttggct atcccgaaac
     1441 agtatttgct gagtgaaacc tgccggatat gttttgaaaa catgattttg ataaatggtg
     1501 aatgtccgga cttttcctgc ttcacttcag caataaccag ttccggccat tcgtgccaca
     1561 catcgtcacc gatattgtaa ctgaggtcaa aatcaatcgt aaccctttcg gatagtaact
     1621 tatcgaccat tgtaatgcgt ttgcatttta tgcgaattgc ttttttaagc gtttcggccg
     1681 gaatatctgt tcgtctttcg agcaattcca gtgcaatccc cgagattata tccgtttttt
     1741 cgggaagttg aatacgtttc ttgacggttc tattcccatt ggttttgaat tttatttcga
     1801 aaaaatggat gtcggagtac atatatgtcc gcatacggag tttgaagcgg tttgtacggc
     1861 cgttgtgatg agcggaatat aactggtaat cttcggtatc aaaatacaaa ctgtcgtaat
     1921 tttcgaaacg tctttcgttg atagttagtg ttctgtaaaa ttcagctgct ttagacagaa
     1981 tatctgccaa ttcgtcctta tgaaagacgt atttaacatc tgtccgattg agtagcttca
     2041 cactgtccat ttcggccaat gatatcggtt tcagttcatt gagtatctca agaaattttt
     2101 ccattcaacg tataaaaaat ataaggcgcg ttttttttca aatagatttt tggttatatc
     2161 acttttttgt aacaaaaatg taatatcggc aaaaaacgca caaagttaat catttaaatt
     2221 gtgcattcag tatgtttttt attctaaatg attatcagta taacacattt atcaattttt
     2281 attcagtaaa tgaacagttt tagcccctga tcttagagtg tgagcgtcga tactttagat
     2341 gttcctgtag taaacgattt ttttaa
//
LOCUS       DAOPZK010000225         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_71081_length_2235_cov_22.057339, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000225 DAOPZK010000000
VERSION     DAOPZK010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71081_length_2235_cov_22.057339"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKH68_09190"
     CDS             complement(<1..601)
                     /locus_tag="PKH68_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNX13148.1"
                     /translation="MTIIEKILARHSNQATVKPDDIIDVTIDTRAARDFGGANVVKNL
                     LDNGLSVADPAKTIFTFDCNPGGSDQKYAANQHLCRQYARENNIRVYDIDAGIGTHQA
                     IDGGLVWPGSTFVSTDSHANIMGSIGCFGQGMGDQDIAATWAKGYVWFKVPYSVKINL
                     TGKRPANVSAKDIVLNLLGIFGANKLLGTSVEVYGEEADK"
     gene            complement(707..1072)
                     /locus_tag="PKH68_09195"
     CDS             complement(707..1072)
                     /locus_tag="PKH68_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNX13149.1"
                     /translation="MSSLEDLEAWKQARELRKEISKLTKSFPKEEKFRLTDQLIRASR
                     SCTANIAEGYGRFHYQESIQYCRQSRGSMYECLDHIYCALDEDFITEEICSKIRTQIM
                     NCIKILNGYILYLKNQKNN"
     gene            complement(1161..1721)
                     /locus_tag="PKH68_09200"
     CDS             complement(1161..1721)
                     /locus_tag="PKH68_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNX13150.1"
                     /translation="MIKPEFIEKAKQLWPELDWIQNADLREKTTHTWALALQKSVLTV
                     DDLNTIPFTLLAGPDLRVTFMGHKRAVVHIAKDCGEQMNKFFKEDLPVNMDVLIAGAI
                     LADVGKLLEYEMKDGKSVQGKYGKYLRHPFSGVSLAEQCGVPAEVCHIIATHAGEGDM
                     VKRTTEAFVVHHADFMTFEPFRDRLK"
     gene            complement(1808..>2235)
                     /locus_tag="PKH68_09205"
     CDS             complement(1808..>2235)
                     /locus_tag="PKH68_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNX13151.1"
                     /translation="MMWLTKNPENYHVIVSENMFGDIVSDAFAGLIGGLGFATSAQFN
                     PDSGVAVFEPTHGSAPKYADYEVSIVNPIAMVLSAVMMLEHLNEMEIAERIRKAVGEV
                     VAEGKIRAYDMLKMKGTPEVITQGAASTTQMADAIIAKL"
BASE COUNT          561 a          472 c          477 g          725 t
ORIGIN      
        1 gtttgtcggc ttcttcgcca taaacttcta cggaggttcc caataattta ttggcaccga
       61 aaatgccaag gagatttagt acaatgtctt tggctgatac atttgccgga cgttttcctg
      121 taaggttgat tttcacagaa taggggacct taaaccacac atagcctttg gcccatgtag
      181 cggcgatgtc ctgatcgccc atgccctggc cgaaacagcc gatggagccc atgatgttgg
      241 catgtgagtc ggttgataca aaggtgcttc cgggccatac aagaccgccg tcaatagcct
      301 gatgtgtgcc aatgcctgcg tcaatatcat aaaccctgat attgttttca cgggcatatt
      361 gcctgcatag gtgctggttg gccgcatact tctgatcgga accgccgggg ttgcagtcga
      421 aagtaaatat ggtctttgca ggatcggcaa cgctgagtcc gttatccaaa aggtttttta
      481 ccacgttggc tccgccaaaa tcacgggccg cgcgggtatc aatagttaca tcgataatgt
      541 cgtcaggctt cacagtggcc tggttggaat gtcttgcgag gatcttttct attattgtca
      601 taggttggga tttttttatt ttttaaatta atattaagtt gattaagtta attaagttaa
      661 ataggttgat tagggaagag gggagaaaaa gtaaaatagt tttcagttaa ttattttttt
      721 ggtttttcaa atataatata taaccgttca ggatttttat gcaattcatt atctgagtcc
      781 ttattttact gcaaatttct tcagtaataa aatcttcatc caaggcacaa taaatatgat
      841 caaggcattc atacatagaa cctcttgatt gacggcaata ttgaatgctt tcctgatagt
      901 gaaatctgcc atatccttca gcgatatttg ctgtacatga tcttgaggct cttatgagtt
      961 gatccgttaa cctgaatttt tcttcttttg gaaaagattt tgtgagtttt gaaatttctt
     1021 ttctcaattc tcttgcctgt ttccatgctt ctaaatcttc taaactgctc atagtactcc
     1081 taatttactt aattaactca tttaacttaa ttaacttatg caactcattt gacttatttg
     1141 acttattcga ctcatttaac ttattttaac cggtccctaa acggctcaaa agtcatgaaa
     1201 tcagcatgat gtactacgaa agcctctgtg gttcttttca ccatgtcgcc ttcgccggcg
     1261 tgtgtggcaa tgatgtggca aacttctgct ggaactccgc actgttctgc caaagatact
     1321 ccgctgaagg gatgacgcag gtatttcccg tattttccct gtacggattt tccgtctttc
     1381 atttcgtatt ccagcagctt accaacgtct gcaaggatag ctccggcaat caatacatcc
     1441 atgtttacgg gaagatcttc tttgaaaaat ttgttcatct gttcgccaca gtctttggca
     1501 atgtgaacga cagcacgttt atgccccata aaggtcactc tcagatcggg tcctgcgagc
     1561 aaggtaaacg gtatcgtgtt caaatcgtca acggtgagca cgcttttttg aagtgcgagg
     1621 gcccaggtgt gggttgtttt ttcgcggaga tcggcgtttt gtatccaatc cagctccggc
     1681 cataattgtt ttgctttttc gatgaattct ggttttatca tgattaaaag tttaaattaa
     1741 tgtttttttt agcaaaatgg ctgttctgta aagagcagcc attttggttt aatgaaaata
     1801 aaaatgatta taacttcgca atgatggcgt cggccatttg tgtggtcgaa gcagcgccct
     1861 gggttattac ttccggagtg cctttcatct taagcatgtc gtaggctctg atttttcctt
     1921 cggccaccac ttcgccgacg gcttttcgga tgcgttcggc aatctccatt tcgttgaggt
     1981 gttctagcat catcacggcc gaaagtacca ttgcgatagg atttactatg gaaacttcgt
     2041 aatctgcata tttaggcgct gatccgtgtg tcggttcaaa aacagccaca cccgaatcgg
     2101 ggttgaactg tgccgaggtg gcaaagccca aaccacctat cagtcctgca aaggcgtcgg
     2161 aaacgatatc gccgaacatg ttttcagaaa cgatcacatg atagttttca ggattcttgg
     2221 tcagccacat catca
//
LOCUS       DAOPZK010000226         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_71568_length_2225_cov_23.265899, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000226 DAOPZK010000000
VERSION     DAOPZK010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71568_length_2225_cov_23.265899"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKH68_09210"
     CDS             <1..432
                     /locus_tag="PKH68_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNX13152.1"
                     /translation="DHALATPSLAKQITFVSAFHINADEPAMFEYSGSNYQGNMYRCS
                     DHDPVVVGIALSGSSDIDTVPSGKNVKIYPTLVTNTFTVKGAENFYIQIFSVNGVKIY
                     ENQVASSVAEYDVRTLGLSAGAYVVRVLGNGTIGRQMIIVR"
     gene            515..596
                     /locus_tag="PKH68_09215"
     tRNA            515..596
                     /locus_tag="PKH68_09215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:549..551,aa:Leu,seq:tag)
     gene            complement(733..1146)
                     /locus_tag="PKH68_09220"
     CDS             complement(733..1146)
                     /locus_tag="PKH68_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HNX13153.1"
                     /translation="MKKLLNLMAFIAIALLVSNCGSNEAKTPATLEKSFYSELQKGNY
                     EKAADLIIKNLSTDKVMTAEESAQLVKSFTEKAKQSTEAKGGVKSFEVVEEKISEDGL
                     SATVSTKVVYGNGTESTEQSKYVKKDNVWKLSMGK"
     gene            complement(1180..1797)
                     /locus_tag="PKH68_09225"
     CDS             complement(1180..1797)
                     /locus_tag="PKH68_09225"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNX13154.1"
                     /translation="MINSTNRFIIRGLCILSVLINACSHSAPGRQFTDIPLAEGDLVF
                     RKGIGAKTQAVLQVDSLGIYSHSGIVVVQNGVFKVVHITPGERKEGETGDKIKAEPIN
                     EFWSEDRAQTGAVYRLKNNLTGSQAARQALRMLGKGILFDHDYELSDTTLMYCTELVW
                     YVYKLAGTDISFGKRSILNVPLYSGTYILPSDIYRNKEFQLIYKF"
BASE COUNT          605 a          457 c          461 g          702 t
ORIGIN      
        1 gatcatgcac tggctacacc atcgctggcc aaacagataa cgtttgtttc agcattccac
       61 atcaatgccg acgagcctgc tatgtttgaa tactccggat caaactatca gggtaatatg
      121 tatcgttgtt cggaccacga tccggtggtg gtaggtatcg ccttatcggg cagttcggat
      181 attgacaccg taccatcggg caaaaatgta aagatttatc cgaccctggt tacaaacact
      241 ttcactgtga aaggcgccga aaatttctac attcagatat tctccgtgaa tggggtgaag
      301 atctacgaaa atcaggttgc atcctccgtg gctgaatacg atgtccgcac gctcggttta
      361 tcggctggag cttacgtggt acgcgtactg ggcaacggta ccatcggacg tcagatgatc
      421 attgtacgat aaggaaaaaa tttagttttg tcctttcgtt gataatttga aaataagttg
      481 tatctttgca cccgttattg aaaaaacgac acctgcggtt gtggcgtaat tggtagccgc
      541 gccagactta ggatctggtg ccgagaggcg tgggggttcg agtcccttca gccgcacaaa
      601 aaaactccct ttggaaatat tccagaggga gtttttttaa tatattgtac cagaataaaa
      661 atttaatcag atttgatttt atattaaatt ccgaaatttc cggtatttat cgcgcatcgg
      721 tttcaattcc tgttattttc ccatcgacaa tttccaaacg ttgtctttct ttacgtattt
      781 cgattgttcg gtactttcgg tgccattacc atacaccact tttgtggaaa cagtggcgct
      841 cagtccatct tccgatattt tttcttccac tacttcaaaa ctttttactc caccctttgc
      901 ttcggtgctt tgttttgctt tttcggtgaa cgattttacg agttgagcgc tctcttcagc
      961 tgtcatcacc ttgtcggtac tgaggttttt gatgatcaga tcggctgcct tttcgtaatt
     1021 ccctttttgt aattcgctgt agaatgattt ttcaagggta gcaggagttt tagcttcgtt
     1081 gctgccacag tttgaaacca ataaggcaat ggcaatgaat gccattaagt ttaagagttt
     1141 tttcatgttg ataaattttt aaaattagac attcgagaat cagaatttat atattagttg
     1201 aaattctttg ttcctgtaaa tatcggaagg cagaatatag gttccggagt aaagtggcac
     1261 gttcagaatg ctccgtttgc caaaagaaat gtcggttcct gccagtttgt agacatacca
     1321 caccagttcg gtgcagtaca tcagcgtagt gtcgctcagt tcatagtcgt ggtcaaacaa
     1381 tatgccctta ccgagcattc gcagtgcctg tcgggctgcc tggctgcctg tgagattatt
     1441 tttcaaccga taaacagccc ctgtctgcgc ccggtcttcg ctccagaatt cgttgatcgg
     1501 ttcggctttt attttatcgc ctgtttcgcc ctcttttcgt tcaccgggtg taatgtgtac
     1561 taccttaaaa accccgtttt gtacaactac aatccccgaa tgactgtaaa tgcccagtga
     1621 atctacctga agaaccgcct gagttttggc accaatccct tttcggaaaa ccagatcacc
     1681 ctcggccaat ggaatatcgg taaactgtcg gcccggagca gaatgggagc aggcatttat
     1741 caacaccgat aaaatgcaaa ggccccggat aataaaccgg ttggtgctgt tgatcatgga
     1801 tgcttatatt gcttaatact ggttgttgct taatcgttat tcacactgat ttttttatta
     1861 caataatttt aaaaaggaaa gaccacaaac ccctatgcta tcaacaattg gattatctaa
     1921 gttaaagctg gataaacaag aaaattggag gtgattttat tctgtattgg ctgctgcaac
     1981 atatatggat actttagtgt cagcgataag aggttctttg ttgactgtac gaatcataaa
     2041 agtacgtcgc aaacttacgg aaaataattt tatatttcaa taagaaattc ataaaaaata
     2101 caacaaatat taaggtttca atagatttca aatcacaaga tataaaaaca ggcgggatag
     2161 tggatgtgtg atgaccgtat attgatttgc tttcaaaaaa ttgtatcttt gatctgataa
     2221 acaac
//
LOCUS       DAOPZK010000227         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_72166_length_2212_cov_25.662958, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000227 DAOPZK010000000
VERSION     DAOPZK010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72166_length_2212_cov_25.662958"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1150
                     /locus_tag="PKH68_09230"
     CDS             341..1150
                     /locus_tag="PKH68_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13155.1"
                     /translation="MENANPNKKNDLHRENEELKKKLEEEFGASKGWTNPDLSPEMEN
                     EFLNHIMAFENAWKDAKQITLYEFLGKPAFRRIDELQPDEISTELERIYSLMLEHQVR
                     LDTICEVSDYELYRFITEELFLEEKDDMRVPGMMSSYIYEEFYPNHEYDLNRHSEEFL
                     NSYLDKTSERYLHYLNSEAEKQQWHTHFRDTFSRFELRKSEIINVSFNLETEMAQVDF
                     ECDFTAQVEGSRDVFQFAGKGKINFVYKWDYWTVDSVEFPKPHNGLSGIQN"
     gene            1264..2106
                     /locus_tag="PKH68_09235"
     CDS             1264..2106
                     /locus_tag="PKH68_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNX13156.1"
                     /translation="MNMKNKIQLFEEKKVRTVWDDETEEWYFSVIDVVEVLTDSDRSR
                     KYWNDLKKKLKTEGSELSEKIGQLKLKSPDGKMRLTDVANTDQLFRLIQSIPSPKAEP
                     FKLWIAQVAKERLDQMQDPELSIEQAMLDYKRLGYSDNWINQRLKSIEIRKDLTDEWK
                     SRGLEEGLHFATLTDIIYKTWADKTAKEYKQFKGLKKENLRDNMTNTELVLNMLAELS
                     TKRISEATNPEAMDEHAEVAKQGGEIARNARLELEAKTGEKVVSPLNAKKGILNKKPD
                     ELKK"
BASE COUNT          795 a          400 c          462 g          555 t
ORIGIN      
        1 gtgtatcaaa atacctggtg gtacaaaatt agttttaggt ttaagaagaa atttgggtag
       61 taaaagctga ccaagaaaat ctgcagcgtg gcatagcgat ataccacgct gatttttttt
      121 atttccttca ctctgtaata aatgaattat accggtccat tttgcttgga attatttatg
      181 aaatgatttt tgatattatt tcaaatgtcc accagtagat aagaattaat ccttatattt
      241 gttgagcgta tgacagttgt ggcacatgct taaataatca tatataaaac ttctggcaaa
      301 acaaaaattt tcgaacaatt aaaattaaac aaatataacc atggaaaacg ccaatccaaa
      361 caaaaaaaac gacctgcatc gcgaaaacga ggaactgaaa aaaaagctgg aagaagaatt
      421 cggggcatca aagggctgga ctaatccgga cctgtcccct gaaatggaaa atgaattttt
      481 gaaccacatc atggcatttg agaatgcctg gaaggatgca aagcaaatta ccctgtacga
      541 atttctgggg aaacccgcat ttcgacgaat agacgagctg caaccggatg aaatcagtac
      601 cgaactggaa cgcatttact cgttgatgct tgaacatcag gttcggttgg ataccatttg
      661 tgaagtgtcg gactacgaac tttaccggtt tatcaccgaa gaattgtttc tcgaagaaaa
      721 agacgatatg cgcgttcccg gtatgatgtc gagctacatc tacgaagaat tttacccaaa
      781 ccatgaatat gatctcaaca ggcattcgga ggaatttttg aattcgtatc tggataaaac
      841 ttcagagcgc tacctgcact acctgaatag tgaagccgaa aagcagcaat ggcacaccca
      901 tttcagagat acattcagcc ggtttgaact ccgaaaatca gagattatca atgtgagttt
      961 taatctggaa accgaaatgg cgcaggttga ttttgaatgc gacttcacag cccaggtgga
     1021 aggttctcgg gatgtatttc agtttgcagg aaaagggaaa atcaacttcg tatataaatg
     1081 ggattactgg acagtggatt ctgtggaatt tccaaaacct cacaacggct tgtctggtat
     1141 tcaaaattga taaatacccg aattattagc ggtaaataaa ataaatcttt tcgataatgt
     1201 aaccggtttc gaaaattgct gaatgaaacg ggttacagga aaaatgaaaa cacaaaacat
     1261 gccatgaata tgaaaaataa aattcaactt ttcgaagaaa aaaaagtccg tacggtgtgg
     1321 gatgacgaaa cagaggaatg gtatttctca gttatagatg tagtagaagt gctgacagat
     1381 tcggatcgtt cacgtaagta ttggaatgat ctgaagaaaa agcttaaaac agaaggaagt
     1441 gaactgtccg aaaaaatcgg acagttgaaa ttgaaatctc ccgatggcaa aatgcgcctc
     1501 acagatgttg ctaataccga tcaactcttc cgtttaatcc agtccattcc atcgcctaaa
     1561 gcagaaccct tcaaactgtg gatagcacag gtagccaaag aacgcttaga tcaaatgcaa
     1621 gacccggaat tatccattga acaggccatg ctggattata aacggttggg atattctgac
     1681 aattggatca atcaacgcct gaaaagcatt gaaatacgaa aagacctgac cgacgaatgg
     1741 aaatcgagag gattggaaga aggtcttcat ttcgctaccc tgacggatat tatttataaa
     1801 acctgggcag ataaaacggc taaagaatac aagcaattca agggattgaa aaaagaaaat
     1861 ctccgcgaca acatgacgaa tacagagctg gtactgaata tgctggcaga actttcaaca
     1921 aaacggattt cggaagctac aaatcccgaa gctatggatg aacatgctga agtagccaaa
     1981 cagggaggtg aaattgcccg caatgcccgt ctggagctgg aagcaaaaac cggtgagaag
     2041 gtggtatctc cgctgaatgc taaaaaaggc atactgaata aaaaaccgga tgagctaaaa
     2101 aaataaaaag taaagcaatc ataattttca aaatagccag cttgatgatt ttaccctttt
     2161 aaaagagcta ttttcaataa accaaacttt caattaatta taccgagtga aa
//
LOCUS       DAOPZK010000228         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_73098_length_2194_cov_20.641889, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000228 DAOPZK010000000
VERSION     DAOPZK010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73098_length_2194_cov_20.641889"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1108)
                     /gene="ribD"
                     /locus_tag="PKH68_09240"
     CDS             complement(41..1108)
                     /gene="ribD"
                     /locus_tag="PKH68_09240"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNX13157.1"
                     /translation="MLSTDEKYMARCLHLAQFGGGYVSPNPMVGAVLVCNDRIIGEGY
                     HRRIGEAHAEPNAIASVTDKDLLPQSTLYVNLEPCSHYGKTPPCANLIVQKGIKRVVI
                     GTLDPNPKVSGRGVEILREAGIEVIAGILENECRHLNRRFFTFQLQKRPYIILKWAQT
                     QDGFIDHNRTDNSMHALKISNSITQQLTHKMRSENMAIMVSTNTVLLDNPSLTLRYWY
                     GKSPLRIAIDRKDIIPESYIIKDGKVPTIIFSRKKSTSKSNPEYIRIDDHDDNLPIIL
                     GELYNHDIHSILVEGGAKLIKSFIASGLWDEANIEISAISIGEGVAAPVLTNAVEVSR
                     NIIDNHKWLHYFNPENPYPFV"
     gene            1352..2107
                     /locus_tag="PKH68_09245"
     CDS             1352..2107
                     /locus_tag="PKH68_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNX13158.1"
                     /translation="MKKILLSLVIALGLTSCAELLQMAGTMGTASVPVTEAETASGLK
                     ESLNVGIVNAVGFLGIENGFYNDALLKILLPEEAQPIIKNLKLIPGGKELVDKAVLSL
                     NRTAEDAVKEATPIFKKAITSMTIGDAFGILFGGQNAATNYLKEKTYTDLQAAFAPKV
                     RVSLGKPLVGDVSTTKSWNALTSAYNQVAATPVGVVANLKPVNVNLEEYVTGKALDAL
                     FIKVAAEEKAIRENPVARITSLLKRVFGQLDKK"
BASE COUNT          635 a          438 c          486 g          635 t
ORIGIN      
        1 taaatataaa atactgctgt aatgataact aaaaaatgaa tcatacgaac ggataaggat
       61 tttcagggtt aaaataatgc agccatttgt gattgtcaat gatgtttctg cttacttcca
      121 cggcatttgt caaaaccggg gcagctactc cttcaccgat tgaaatcgca gaaatttcaa
      181 tattagcttc gtcccacaat ccggatgcga taaaactttt tattaatttg gcacctccct
      241 ccaccagtat ggagtgaatg tcgtgattgt ataattcacc gagaataatt ggcagattat
      301 cgtcgtgatc atctatgcgt atatactccg gattggattt tgaagtcgat ttttttcgtg
      361 aaaatataat ggtaggtact tttccatcct tgataatgta gctttcggga atgatatctt
      421 ttcggtcaat agcaatgcgt aagggcgatt ttccgtacca atagcgcagg gtgagcgatg
      481 gattgtcgag cagtacggta ttggtgctca ccatgatagc catattctcc gagcgcattt
      541 tatgtgtgag ttgctgggtg atggaattgg aaattttcag ggcatgcata ctgttatcag
      601 tccggttgtg atctataaaa ccatcctgtg tttgtgccca tttcaatatg atgtaaggtc
      661 ttttttgtaa ctgaaaagtg aagaaccgcc ggttgagatg acggcattca ttttccagta
      721 ttcctgcaat aacctcaatg ccggcttcac gcaagatttc cactccgcga cccgaaactt
      781 tcggatttgg atctaatgta ccaatgacaa cccgttttat ccctttttgc actattaaat
      841 tggcacaagg cggtgttttg ccatagtgtg agcagggctc cagatttacg tagagagtcg
      901 attgtggcaa taagtctttg tcagtcaccg aagcgatggc attgggctcg gcatgtgcct
      961 cacctatacg acggtgatat ccttcgccga tgatgcgatc gttgcaaaca agcacagcac
     1021 ccaccatcgg attgggtgat acataaccgc ctccgaattg tgccagatgc agacagcggg
     1081 ccatatattt ttcgtcagta ctcagcattt tatttgttaa aaaaatgtcg tgacaacaaa
     1141 aatacgaact ttcgggcaat ttaccggctt ataaatgttt ttttattcca aaattcatta
     1201 ttcagaaaat taatcttact tttatggaat gatttttgaa tgaataacaa ctaatcggtg
     1261 cattacggaa taattcaccc gtataaaccg gttattttcc gttgaaatag aaatttcaaa
     1321 acataatata aaacataaaa cagaaaaaag tatgaaaaag attttactga gtttggtaat
     1381 tgcactggga ctgacttcct gtgccgaatt gctgcaaatg gcgggtacaa tgggtactgc
     1441 ttcggtacct gtgaccgaag ccgaaactgc cagcggattg aaagaatcgc tgaatgtggg
     1501 aatagtgaat gctgtgggtt ttctgggtat tgaaaacggt ttttacaacg atgcgctgct
     1561 caaaattctt ctacccgaag aagcacaacc cattattaaa aacctcaaac ttattccggg
     1621 tggaaaagaa ctggtggaca aagcagtgct ttcgctcaac cgcacggccg aagatgctgt
     1681 gaaagaagcc acacccattt tcaaaaaagc catcacgagc atgaccatcg gtgacgcatt
     1741 cggcattcta ttcggcggac aaaatgcagc taccaactac ctgaaagaaa aaacctatac
     1801 cgatttgcag gctgcatttg cacccaaagt gagagtatcg cttggaaaac cgctcgtggg
     1861 tgatgtttcg accaccaaat cgtggaatgc actcacttcg gcatacaatc aggtggcagc
     1921 tactccggtt ggcgtggtag ccaatctgaa accggtgaat gttaacctgg aagaatatgt
     1981 aaccggtaaa gcactcgatg ccttgtttat aaaggtggca gcggaggaaa aagctattcg
     2041 cgaaaatcct gtagcacgta ttacttcact cctcaaacgg gtgttcggac agctggataa
     2101 gaagtagtta tttagttatt gagttattca gttattgagt tatttgaccc gaacctttgt
     2161 gattgggtta atggtactga agctggtttg gaat
//
LOCUS       DAOPZK010000229         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_75309_length_2151_cov_33.267176, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000229 DAOPZK010000000
VERSION     DAOPZK010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75309_length_2151_cov_33.267176"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..637
                     /locus_tag="PKH68_09250"
     CDS             23..637
                     /locus_tag="PKH68_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNX13159.1"
                     /translation="MKKLIIPVLIAAFLWFLMFSPWTSQFFNFWIAMAISATILITIS
                     LTQRKSILDQFNFNWKAIGFGLLSAVVLWMVFYLGDFFSKMLFDFARPQVDSIYAMKE
                     GQNKILLILGLLFIIGPAEEIFWRGYVQDRLIGKYGEWTAFIITTLIYALVHIWSFNF
                     MLVMAALICGIFWGLMYRYSKNLVPLVISHAVWDVAVFILFPIM"
     gene            830..>2151
                     /locus_tag="PKH68_09255"
     CDS             830..>2151
                     /locus_tag="PKH68_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNX13160.1"
                     /translation="MKPNFKDININDFKAGAVAPEAEGSWLTPELISVKPSYTKEDLS
                     GMDHLNYAAGVAPFLRGPYSSMYVMQPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLS
                     VAFDLATHRGYDADHERVVGDVGKAGVSICSVEDMKVLFDGIPLNKMSVSMTMNGAVL
                     PVLAFYINAGLEQGAKLEEMAGTIQNDILKEFMVRNTYIYPPKFSMKIIADIFEYTSK
                     NMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRAGVNAGMDIDAFAPRLSFFW
                     AIGMNHFMEIAKMRAARMLWAKIVKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNVG
                     RTCIEAMGAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQQETDITREVDPWA
                     GSYYVESLTNELAEKAWALIEEVEKLGGMAAAIETGIPKMRIEEAAART"
BASE COUNT          611 a          438 c          474 g          628 t
ORIGIN      
        1 ctaattttat tgcaggttta ctatgaaaaa actaatcatc cccgtactta tagccgcgtt
       61 tttgtggttt ttgatgttct caccctggac aagccagttc tttaatttct ggattgcgat
      121 ggccatttca gcaactattc tgataacaat tagtctgaca caacgcaagt cgatactgga
      181 tcagttcaat ttcaactgga aagcgattgg ttttggtctg ctgtcagccg ttgtgctctg
      241 gatggtgttt tatttgggcg acttcttttc taaaatgctt tttgattttg cgcggcctca
      301 ggtcgacagc atctacgcaa tgaaagaagg acaaaataaa atattactga tattgggttt
      361 actgtttatc atcggtccgg ccgaagaaat attctggcgt ggttacgttc aggataggtt
      421 aatcggaaaa tacggagaat ggacagcctt tatcattacc acactcattt atgcactggt
      481 gcatatctgg tcatttaatt ttatgctggt aatggcggct ttgatttgcg gtattttctg
      541 gggattgatg tacagataca gcaaaaatct tgtaccactg gttatatcac atgctgtatg
      601 ggatgtggcg gttttcattc tgttcccgat tatgtaaaac ccgggaaacg gaaacaaagc
      661 attaaataaa aaatatattc attcaatgaa ttttgagagt attcagaata aaatcattga
      721 acttcaaaaa atgataacag gatttaaaaa taattggatt aagaacgaaa aatcatgacc
      781 ttattatcgg tcttttatct ttgttcttta atctttaatc tagaataata tgaaaccaaa
      841 ttttaaggat atcaatatca acgattttaa ggcaggtgca gtggcacccg aagccgaagg
      901 tagctggctg acaccggaac tgatttccgt gaagccatct tatacgaaag aagacctttc
      961 cggcatggat catctcaatt atgctgccgg agtagcgcca ttcctgcgtg gtccgtacag
     1021 ctccatgtat gtaatgcagc cctggactat tcgtcagtat gcaggttttt ctaccgccga
     1081 agaaagtaac gctttctatc gccgcaatct ggcagccggt cagaagggac tttccgttgc
     1141 ctttgacctg gcgacacacc gcggttacga tgccgatcac gaacgcgttg tgggcgatgt
     1201 aggaaaagca ggtgtatcca tctgctcggt tgaagatatg aaagttttgt ttgatggtat
     1261 tccattgaat aaaatgtctg tttccatgac aatgaatggc gctgtacttc ctgtgctggc
     1321 attctacatc aatgcaggtt tggaacaggg tgcaaaactc gaagaaatgg caggaactat
     1381 tcagaatgat attctgaaag aatttatggt gcgtaataca tatatttatc caccaaaatt
     1441 ctccatgaaa attattgctg atatttttga atatacatcc aaaaatatgc ctaagttcaa
     1501 ttccatttcc atttcaggtt accacatgca ggaagccggt gcaacagccg atattgaact
     1561 tgcctatacg cttgccgacg gacttgaata tctccgtgcc ggtgtgaatg ccggaatgga
     1621 tatcgatgcg ttcgccccgc gtttgtcatt cttctgggct atcggaatga accactttat
     1681 ggaaattgcc aaaatgcgtg cagcacgtat gctttgggct aaaatcgtaa aacagttcaa
     1741 tcctaaaaac ccaaaatcac tggcattgcg tactcactcg caaacttccg gctggtcgct
     1801 taccgaacag gatccgttca ataatgtggg tcgtacctgt attgaagcaa tgggagcagc
     1861 tctcggtcat acacagtcac ttcataccaa tgcgctggac gaagccattg cgttgccaac
     1921 cgatttctcg gcacgtattg cccgtaatac acagatttat atccagcagg aaactgatat
     1981 cacccgcgaa gtcgatccat gggcaggttc ctattatgtg gaaagtctga caaacgaact
     2041 ggcagaaaaa gcctgggcat tgattgaaga agttgagaaa cttggtggta tggcagcagc
     2101 tatcgaaacc ggaattccaa aaatgcgtat tgaagaagcg gcagcccgta c
//
LOCUS       DAOPZK010000230         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_75637_length_2145_cov_22.446890, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000230 DAOPZK010000000
VERSION     DAOPZK010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75637_length_2145_cov_22.446890"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PKH68_09260"
     CDS             <1..1160
                     /locus_tag="PKH68_09260"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNX13161.1"
                     /translation="IATRDAFLNAMTLDIAMGGSTNTVLHLLAIAHEAEVDFTMDDID
                     MLSRKTPCLCKVAPNSPKYHIQDVNRAGGILGILGELADLPPNPLKGELSSSLKTGAY
                     EVANTSSPLGRLGGALYLNVKRVDGLTLAEAIEKYSVTKENIHTEAERIYRSAPGGKF
                     NLVMGSQTSTYKTLDTDRSEGCIRDLEHAYTKDGGLGILKGNIAQDGCVIKTAGVDES
                     IWKFIGPAKVYNSQDDACEGILKGKVVSGDVVVITHEGPKGGPGMQEMLYPTSYIKSK
                     HLGKECALITDGRFSGGTSGLSIGHISPEAAAGGNIGLLHDGDIIEIDIPNRTINARV
                     SDEEFAERRKAEEARGKDAFTAAGRDRKISKALKAYASMVTSADKGAVREI"
     gene            1276..>2145
                     /locus_tag="PKH68_09265"
     CDS             1276..>2145
                     /locus_tag="PKH68_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNX13162.1"
                     /translation="MAQKLNGAHAFMQALIQEGVETIFGYPGGAIMPVFDALYDYSDK
                     VNHILTRHEQGATHAAQGYARVSGKVGVALVTSGPAATNAITGVADALLDSTPLIVVT
                     GQVGAALLGTDAFQEIDVVGITQPITKWSYQIRKAEDVAWAVARAFYIARSGRPGPVV
                     LDFAKSAQSQEVEFDYKPCTFIRSYIPVPAIKEEQIEKAAELINQAKKPFALVGQGVI
                     LANAEKELLEFLEKADIPAASTLLGLSAMPTDFRLNKGFLGMHGNVAPNMKTNECDVL
                     IAIGMRFDDRITGD"
BASE COUNT          627 a          457 c          544 g          517 t
ORIGIN      
        1 gcatcgccac ccgcgatgca tttctcaatg ccatgaccct ggatattgcc atggggggct
       61 ccaccaacac cgttttacac ctgctggcta tcgctcacga ggcggaagtg gatttcacaa
      121 tggatgatat cgatatgctt tcgcgaaaaa caccctgctt gtgtaaagta gctccgaact
      181 cacctaaata tcatatacag gacgtgaaca gagccggagg tatactgggg attttgggag
      241 aactggcaga cttgcccccc aaccccctga agggggagtt aagctcgtct ctaaaaaccg
      301 gtgcttatga agttgcaaac acttcctctc ctttggggag gttaggtggg gctttatatc
      361 taaatgtgaa gcgggtggat ggattgacgt tagcagaggc gattgaaaaa tactcggtta
      421 caaaagaaaa tattcacacc gaagcagagc gtatttacag aagtgcaccc ggaggaaaat
      481 ttaacctggt gatgggctcg cagacttcga catacaaaac actcgacacc gaccgctcgg
      541 aaggttgtat ccgtgatttg gaacacgcct acacaaaaga tggcggactt ggcatcctga
      601 agggaaacat tgcacaggac ggatgtgtta taaagactgc cggcgtggac gaaagtatct
      661 ggaaattcat cggtcctgct aaggtttata actctcagga cgatgcctgc gagggaattc
      721 tgaaaggaaa agtggtttcg ggagatgtgg tggtgataac ccatgagggc cccaaaggtg
      781 gtccgggcat gcaggaaatg ctctacccta cttcatatat caaatcaaaa catcttggaa
      841 aggaatgtgc tctgattacg gatggtcgtt tttcaggcgg cacatcggga ctgtctatcg
      901 gacatatttc acctgaagcg gctgccggag gaaatatcgg gctgttgcac gatggggata
      961 ttattgaaat tgatataccg aatcgcacca taaatgcaag ggtaagtgat gaagaatttg
     1021 ctgaacgcag aaaagcagaa gaagcgagag gtaaagatgc atttactgcc gccggacgag
     1081 accggaaaat ttccaaagcg ctgaaagcct atgctagtat ggtaacttcg gcggataaag
     1141 gagctgtgag agagatttga aaaaattgaa agttatttac aagtcattta aattgttatt
     1201 aattaacttc tattgtaaaa aaaacaatca gggtaattca ggcaaaaaac cgtttactca
     1261 tttataaaac acgatatggc acaaaaatta aatggtgcac atgcatttat gcaggcactt
     1321 atacaggaag gtgttgaaac tattttcggt tatcccggtg gtgcaatcat gcccgtattc
     1381 gacgctttat acgattattc cgacaaagta aatcatattc ttacccgtca cgaacaggga
     1441 gcaacgcacg ctgcacaagg ctatgcacgt gtgtcgggca aagtgggtgt agcactcgta
     1501 acatcgggtc ctgcggccac caatgccata accggagttg ccgacgcatt gctcgacagc
     1561 acgccgctca tcgttgttac aggtcaggtt ggtgcagcat tgcttggtac cgatgcattt
     1621 caggaaattg atgtggtcgg tatcacacaa ccgattacaa aatggtcata ccagatccga
     1681 aaagctgaag atgttgcctg ggctgtggca cgtgcatttt acattgcccg aagcggtaga
     1741 ccgggtccgg tagtacttga ttttgccaaa agtgctcaaa gtcaggaagt ggaatttgat
     1801 tataaaccct gcacatttat ccggagctat attcccgttc cagccataaa agaagaacaa
     1861 atagaaaagg ccgccgaact gattaatcag gcaaaaaaac catttgcact agttgggcag
     1921 ggagtaattc tggcgaatgc agaaaaagag ttgctcgaat ttctggaaaa agcggatatt
     1981 ccggccgctt ctaccctgct cggtctatcg gccatgccca ctgatttccg tctgaataaa
     2041 ggttttctgg gaatgcacgg caacgtggct ccgaatatga aaaccaacga atgtgatgta
     2101 ctgattgcca tcggaatgcg ttttgatgac cgcattaccg gcgac
//
LOCUS       DAOPZK010000231         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_76606_length_2128_cov_21.355041, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000231 DAOPZK010000000
VERSION     DAOPZK010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76606_length_2128_cov_21.355041"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKH68_09270"
     CDS             complement(<1..907)
                     /locus_tag="PKH68_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNX13163.1"
                     /translation="MQTIKIAILGLGGVGGYYGGKLAKRFRDTSHVEIYFIARGEHLE
                     AIKEYGIHIINENEDFFAKPKLATSDAAEIGPVDYLIITTKSYDLKSSIDQIRPCIDN
                     NTVILPLLNGGDITERIREILPGNTVWSGCSYIVSRRTEPGVIRTSGPFLRLVFGYDC
                     GEDERLLSFEKLLLEAEIDAHYSRNIRESVWKKFYFISVSASLTSYLDVTFNQLADNP
                     ERKNLTIAVADEFLKVAKAEGIHFDENTAEEIVNRTEILPAGTTTSMHSDFKSGNATE
                     LETLTGVVVRLAHKHNIPVPLYEMIY"
     gene            complement(1166..>2128)
                     /locus_tag="PKH68_09275"
     CDS             complement(1166..>2128)
                     /locus_tag="PKH68_09275"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNX13164.1"
                     /translation="ETVGNPTLNPAFQHNLRLMFSTYSSTKFSSFNFGLNGSVIKDDL
                     TNNSIYDETGKRYIQTVNSKEMPYNASVFTMFSTPFWKKFNFSNNASFNVRQQYGYTS
                     KNVSTDLIDINHLRLGDLSSTIRYGASDNISLSYTHNVFDISMRGGLSYSNSKNNFNT
                     KANETYDWTAATYLGIRPTSTLTLNTDLNYTTQQGYSSFNQSQWLWNASAELSAFKKR
                     GVFSIKIYDLLRQRQNISQTVGDNYIQYSKSNSLPAYFLVGFTYKISKFKGVSNSDRE
                     NMENMDRFGPGQRMYREGRGDQRTGPGSGGRPPFMGGGGPPPEM"
BASE COUNT          542 a          449 c          473 g          664 t
ORIGIN      
        1 tgtagatcat ttcgtagagt ggaacgggga tgttgtgctt gtgagccagg cgaacaacca
       61 cgcctgtgag cgtttccagt tcggtggcat tacccgattt gaaatcgctg tgcatggagg
      121 tggtggttcc ggcaggtaga atttcggttc ggtttacaat ttcttcggca gtattctcat
      181 caaagtgaat tccctcagcc tttgctactt tcagaaattc gtcagctact gcaatcgtca
      241 aatttttacg ttccggatta tcggccagtt ggttgaaggt tacatccagg tacgacgtga
      301 gtgatgcaga tacagagata aaataaaatt tcttccacac actttcgcgg atatttcggg
      361 aataatgtgc atcaatttcg gcttcgagca gcagtttttc gaaactcagc agccgctcat
      421 cctctccgca atcataccca aacaccaacc ggagaaacgg tccggaagtg cgaataactc
      481 cgggctctgt tcgtcggctt acaatgtatg aacatccact ccacacggta tttccgggca
      541 aaatttcacg tattcgttca gtaatgtcgc caccattgag caaaggaaga atgacggtat
      601 tattatcaat acacgggcgt atctggtcaa tggacgattt caggtcataa cttttagtag
      661 tgatgatcag gtaatccacc ggaccaattt cggccgcgtc ggaagttgcc aactttggtt
      721 ttgcaaagaa atcctcgttt tcattgatga tatgaattcc atactccttt attgcctcca
      781 ggtgttcacc ccgggcaata aaataaattt ccacatgcga ggtatccctg aatcgctttg
      841 ccagttttcc gccgtaatat cctcctactc cacccaatcc gaggatagct attttaatgg
      901 tttgcatatg tttataattt agttatcggc cccctaaccc cctaaagggg gaatattgag
      961 cgtctatcat ttgttgtggg tttttaatca tgtccgtgtg tgtcaaaaat actttacttg
     1021 gacatgtgtg tttcatatgt ttctgttttt taatgttcaa acaagttatg tttgaaaaag
     1081 tttaatctgg atattggaca atgaagatgt ttttataatc cgcaacaaaa aaacaaccgg
     1141 attttcgtcc ggttgcatta tgattttaca tttcgggagg tgggccaccg ccacccataa
     1201 agggaggacg tcctcccgag ccgggtccgg tgcgctggtc gcctcgcccc tctctgtaca
     1261 ttcgttggcc gggaccgaaa cgatccatgt tttccatatt ttccctgtcg ctgttgctca
     1321 cgcctttgaa tttgctgatt ttataggtaa atcctacgag gaaataagct ggtaaagagt
     1381 tggatttgct gtattgtatg tagttatctc ccacggtttg tgatatattt tgacgctgac
     1441 gcaacaaatc gtatatttta attgagaaca cgcctctttt cttgaatgcc gatagctctg
     1501 cggaagcatt ccacaaccac tggctttgat taaagcttga ataaccctgc tgggtggtat
     1561 aattcaggtc tgtgttcagt gtaagcgtac tggtagggcg gatgcccaaa taggtagctg
     1621 ctgtccagtc gtatgtttca tttgctttcg tattgaaatt gtttttggaa ttggaataac
     1681 tcaatccacc ccgcatactg atatcgaata cattgtgtgt gtagctcagt gaaatattgt
     1741 cggatgcacc gtagcggatg gtactgctca aatctcccag tcgcaggtga ttgatgtcaa
     1801 taagatcggt gcttacattt ttggatgtat aaccgtactg ttgacggaca ttgaaagagg
     1861 cattgttgct gaaattaaac tttttccaaa acggagtgct gaacatggta aacacactgg
     1921 cattgtaagg catttctttc gaatttactg tctgaatata ccttttgccg gtttcatcat
     1981 agatactgtt gttagtcaaa tcgtctttaa tgacactacc gttcagtcca aaattaaagg
     2041 aagagaattt agtactgctg taagtggaaa acatcaaccg cagattgtgt tggaaagccg
     2101 gatttaaggt tggatttccc accgtttc
//
LOCUS       DAOPZK010000232         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_77723_length_2108_cov_22.896249, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000232 DAOPZK010000000
VERSION     DAOPZK010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77723_length_2108_cov_22.896249"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKH68_09280"
     CDS             complement(<1..489)
                     /locus_tag="PKH68_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNX13165.1"
                     /translation="MSKIFEPLFLKSVTIKNRIGMSPMCQYSAADGFANNWHLVHYGT
                     RAVGGTGLIIQEATAVAPEGRITPYDLGIWKDEHIEKLKEITAFISSRGAVPAIQLAH
                     AGRKASCDYPTKGGKQLSVENGGWQTVAPSALPFFTDDNPPHVLTENEIRHIVSQFKD
                     AAV"
     gene            740..>2108
                     /locus_tag="PKH68_09285"
     CDS             740..>2108
                     /locus_tag="PKH68_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNX13166.1"
                     /translation="MTQIKERLEALRQAMKNAGVAACIVPGTDPHAGEYIADYWKERV
                     WISGFTGSAGTAAVTLDKAGLWTDSRYFLQGADQLKDSTIDLMKQGLPETPEIIPWLS
                     TELKSGDKVAVNAQMFSVNAYASMKAELKMSGIELVSHDLLKEVWTDRPALPHNPFFV
                     FDVKYSGKSVTDKLILLREELRKAHANVFVMSALDDIAWLFNIRGNDVAFNPVVIAYA
                     LVDEEKAMLFVDPAKCTDASFSYLNDNGVIIANYQDIYTQLREIPTGKAVMYDGNKLN
                     QSLFEAIPAECEKRVGMSPVFKLKSLKNEVEIAGVRKAMIKDGVALTKFFIWLEENVK
                     HGHLTEVSIAEKLLGFRTEQKNFIGESFNTIAGYAGHGAIVHYSASPETASTLKPEGI
                     LLLDSGGQYLEGTTDITRTITLGKPTAKQKKDYTLVLKGHIDLAMAKFPVNTRGSQLD
                     ILARKA"
BASE COUNT          619 a          427 c          500 g          562 t
ORIGIN      
        1 gacagctgcg tctttgaact gactgacaat atgtcggatt tcattttccg taagcacgtg
       61 gggaggattg tcgtcggtga aaaaaggcag ggcagagggt gctaccgttt gccagccacc
      121 attttctact gaaagttgct tgccaccttt agtcggataa tcgcatgaag ctttcctacc
      181 tgcatgagcc agttggattg ccggaaccgc accccgggaa ctgatgaatg ccgttatttc
      241 ttttaatttt tcaatatgtt catctttcca aataccaagg tcgtagggag taattcttcc
      301 ttcgggtgca acggccgtag cttcctgaat gataagtcct gtgccgccta cggctctggt
      361 tccgtaatga actaaatgcc agttatttgc aaatccatcg gcagcggagt attggcacat
      421 gggtgacatc ccgatacggt ttttgatagt aacggattta agaaacagtg gttcgaatat
      481 tttgctcatt gtatttattt tattgattta ttgacttcaa aacaatccgc agcaggaaat
      541 gttttgaacc tgattgaaat ccaatcccaa agataacact atatagagga taagtatgtt
      601 aatcaatttt aataaaaaaa catacgcgga attttatttt atatctttgt gaaaataagc
      661 aaagagacag acggctgtct gagcaaaaag aaaaccccca atcaggtctt caaaaaaaca
      721 aattaaaaac atatcattta tgacacaaat aaaagaacgt ctcgaagcat tgcgtcaggc
      781 aatgaaaaat gcgggtgttg cggcttgcat cgtacccgga acagacccac atgcaggtga
      841 gtatattgcc gattactgga aagagcgtgt atggataagc ggttttaccg gatctgccgg
      901 tacagcagct gttacactgg ataaagccgg attgtggacc gattcgcgtt attttctaca
      961 gggcgctgat cagttgaagg atagcaccat tgacctgatg aagcagggat tgcccgaaac
     1021 gcccgaaatt attccctggt tgtccaccga actgaagagc ggagataaag tggcagtcaa
     1081 tgcgcagatg ttttcggtga atgcttatgc aagcatgaaa gccgagttga aaatgagtgg
     1141 tattgaactt gtttctcatg atttactgaa agaagtctgg accgatcgtc cggctttgcc
     1201 acataatcct ttttttgtat tcgatgtgaa atattcgggc aagtcggtga cagataaact
     1261 cattctgttg cgcgaagaac tccggaaggc gcatgccaat gtattcgtta tgtcggcttt
     1321 ggatgatatt gcctggctat tcaatatccg tggcaatgat gtggctttca atcccgtggt
     1381 gatagcctac gcactggttg atgaggaaaa agcgatgtta ttcgttgatc cggctaaatg
     1441 taccgatgca tcattcagct atctgaatga caacggtgta atcatagcca attatcagga
     1501 tatttacact cagctgagag aaattccaac gggaaaagct gtgatgtatg atggaaacaa
     1561 actcaatcag tcgttgtttg aagcaatacc cgctgaatgt gaaaagagag tagggatgtc
     1621 gccggtattc aagctgaaaa gtctgaaaaa tgaagtggag atagccggtg tgcggaaagc
     1681 catgattaag gatggtgtgg cattgaccaa attctttatc tggctcgaag aaaacgtaaa
     1741 acacgggcat ttgacagaag tatccatagc cgaaaaactg ctcggtttcc ggacagaaca
     1801 aaaaaacttt attggtgaaa gtttcaatac cattgccggt tatgctggtc atggtgcaat
     1861 tgttcattac tcggcaagtc ccgaaacagc ctcgacactg aagcccgaag gaattcttct
     1921 gctggactcc ggcggacaat acctggaagg tactaccgat attacccgaa ccattaccct
     1981 tggaaaaccc accgcaaagc aaaagaaaga ttatacactg gtgctcaaag ggcatattga
     2041 tctggccatg gctaaatttc cggtgaatac ccgcggctcg cagctggata ttctggcacg
     2101 taaagcca
//
LOCUS       DAOPZK010000233         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_78944_length_2087_cov_26.830217, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000233 DAOPZK010000000
VERSION     DAOPZK010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78944_length_2087_cov_26.830217"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKH68_09290"
     CDS             <1..282
                     /locus_tag="PKH68_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNX13167.1"
                     /translation="KKGKLPAETYSINYALEYGTDELEIHKDAIEPDDVVLLHDDLLA
                     TGGSANAALQLLRKFGNNPIYVSFMIELVELNGISLLGDAKDIHSLVKF"
     gene            390..1235
                     /gene="argB"
                     /locus_tag="PKH68_09295"
     CDS             390..1235
                     /gene="argB"
                     /locus_tag="PKH68_09295"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNX13168.1"
                     /translation="MELNTNYTENADNPHSNSPFRGLGGRATLTIIKVGGKIVEEPES
                     LKQLLTDFSLIVGCKVLVHGGGRSATALAAQLGIESKMVNGRRVTDEEMLKVVTMVYG
                     GLVNKKIVAGLQALGVNALGLTGADLNYMRSEKRPIAEVDYGFVGDVKQVDADILSDL
                     ISKSVVPVLAPLTHDKKGNLLNTNADTIAGEAAKALARYFEVTLMYCFEKKGVLADEN
                     DDDSVIENINPATFEKLVAEGIISGGMIPKLENAFDALKSGVKTVVITRADRIHLLSG
                     TKITL"
     gene            1238..>2087
                     /locus_tag="PKH68_09300"
     CDS             1238..>2087
                     /locus_tag="PKH68_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNX13169.1"
                     /translation="MKKLIFPAIFTVMLNGFVFSQEKIMLYPKGPTESNELQTEEKWR
                     DKDFLLNISEPRMVAYPASKDNNCGTAVLICPGGGYSGVSAIKEGEEFARWFNELGVS
                     AFVLYYRMPNAHHEIPLKDAQTALSIIQKRAKEWNINKKKIGIMGFSAGGHLASTVGT
                     HFKNKKERPAFMILGYPVVTMDKNLTHKGSRDNLLGKNPSDELVKLYSNELQVTKKTP
                     PTFIVHTKDDGTVPIANSENLLKSLQENKVPAKLVTYDQGGHGFGMRKKGIPVENWPE
                     ELKGWMK"
BASE COUNT          655 a          428 c          487 g          517 t
ORIGIN      
        1 aaaaagggaa aattaccggc cgaaacttac agcataaatt atgcactcga atatggaaca
       61 gatgaactcg aaatccataa agatgccatt gaaccggacg acgtagtgct cttgcacgat
      121 gatttgctgg ctaccggcgg aagcgccaat gcagcgcttc aacttctgcg aaaatttggc
      181 aacaatccaa tctacgtaag ttttatgatt gaactggtgg aattaaacgg catttccctt
      241 cttggtgatg caaaagatat acattcattg gtgaagtttt aataatatcc ttgcccccta
      301 accctcccga aaaatcggga caggcactga agggggaata ctaaccgaat tgttaaaaat
      361 ttcgacacat attcttctat ttcactaaaa tggaattaaa tacaaattac accgaaaatg
      421 ctgataatcc ccactctaac tcccccttca gggggctggg gggccgtgct actcttacca
      481 ttatcaaagt cgggggaaag attgtggagg aacccgaaag tctgaaacag ttactgaccg
      541 atttttcact gattgtaggc tgtaaagtgc tggtacacgg cggcggtcgc tcggccactg
      601 cattggcagc gcaactcggt attgaaagca aaatggtaaa cggcaggcgt gtaaccgatg
      661 aagaaatgct gaaagttgtt acgatggttt acggcggatt ggtgaataaa aagattgtag
      721 ccggtttaca ggcgctgggt gtaaatgcat tgggattaac gggtgccgac ctgaattata
      781 tgcgttctga aaaacgcccg atagctgagg tggattacgg ttttgttggc gatgtgaaac
      841 aagtcgatgc cgatattctg tctgatttaa tttccaaaag cgtagtaccc gtactggcac
      901 cgcttacaca cgataaaaaa gggaacttac tgaacacaaa cgccgacacc atagccggag
      961 aagccgccaa agcacttgcc agatatttcg aagttaccct gatgtattgt tttgaaaaaa
     1021 aaggagtttt ggcggatgaa aatgacgacg acagtgtaat tgaaaatatc aaccccgcta
     1081 cgttcgaaaa gctcgttgct gagggcatta tttcgggtgg gatgataccc aaactggaaa
     1141 atgcttttga tgcgcttaaa tcgggcgtaa aaacggttgt catcacacgg gcggaccgca
     1201 ttcatctcct ttcgggaact aaaatcacgt tataaatatg aaaaaactta ttttccctgc
     1261 aattttcacc gttatgttga atggttttgt tttttcacag gaaaaaataa tgctatatcc
     1321 taaaggacca acagaaagta atgagttaca aaccgaagaa aaatggcgcg ataaagactt
     1381 tctgcttaat atcagcgaac cccgcatggt tgcttatccg gcttcgaaag ataataactg
     1441 cggtacagcc gttctgattt gtccgggtgg aggctacagt ggagtttcgg ccataaaaga
     1501 aggagaggaa tttgcccgct ggttcaacga gctgggagtt tcagcatttg tgctctacta
     1561 ccgcatgcca aacgctcatc acgaaattcc cctgaaagat gctcaaaccg ccctttcgat
     1621 catccaaaaa cgagccaaag agtggaatat caacaaaaag aaaataggca ttatgggctt
     1681 ttcggcagga ggtcatctgg cttcaacggt tggtactcat ttcaaaaaca aaaaggaacg
     1741 acctgcattc atgattttgg gatatccggt ggtgactatg gataaaaacc tgacacataa
     1801 aggttcacgg gataatttac ttggaaaaaa tccttcagat gaattggtta aactgtattc
     1861 caacgaactt caggtaacaa aaaaaactcc tccgactttt atcgttcaca ccaaagatga
     1921 tggaacagtg cccattgcca acagcgaaaa tctgctgaaa tcgttgcagg aaaataaagt
     1981 tcctgccaaa ctggtgactt acgaccaggg tggacacggt ttcggaatgc ggaaaaaagg
     2041 aattccagtc gaaaactggc ctgaggagct gaaagggtgg atgaagg
//
LOCUS       DAOPZK010000234         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_84860_length_1990_cov_23.226873, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000234 DAOPZK010000000
VERSION     DAOPZK010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84860_length_1990_cov_23.226873"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PKH68_09305"
     CDS             <1..1227
                     /locus_tag="PKH68_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNX13170.1"
                     /translation="QNSLYNLVENLIEVFGIQQWHQETVFLQAFQDVIFKFVNSRNAD
                     LNSFLIWWENNGASQSIATPENQNSFRIMTIHKSKGLDFKVVIMPFCDWFLDAAKNPP
                     LLWCQADTEPFNEFPLLPIDYSSRLVNSIFQKDYFNEQMHQYIDNLNMAYVAFTRARN
                     ELLCFAPQPKNEVPIEIAKMASLSQLILNGISLDQTADTNQISLAENYSPETNSFEIG
                     KPCNAVYTVKPAENELRQMNDYPIADSRNRLKIKHSSRDYWLAGQTMEESRLNYGTIM
                     HDILKKIVTRSDEDKAINEMIVSGKITELESEMIRTEMTKFWNMPETAAWFDPLAKIL
                     NETSILLPTGEQYRPDRLILRDNKAVIVDYKFGDEIMKSHALQIANYENIIRQMGYET
                     ETYLCYVSLNKVVTLS"
     gene            1518..1871
                     /gene="rplS"
                     /locus_tag="PKH68_09310"
     CDS             1518..1871
                     /gene="rplS"
                     /locus_tag="PKH68_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNX13171.1"
                     /translation="MDLLKVAEQAFAVETEKTLPTFKSGDTITVAYRIKEGNKERIQE
                     YRGDVIKITGSGDKKRFTVRKVSGNIGVERIFPMDSPFIESIAINKRGKVRRAKLYYL
                     RKLTGKKARIKERRV"
BASE COUNT          665 a          407 c          393 g          525 t
ORIGIN      
        1 caaaattcgc tgtataatct ggtcgaaaac ctgattgaag tttttggaat acaacagtgg
       61 caccaggaaa cggtatttct ccaggcattt caggatgtaa ttttcaagtt tgtcaactcc
      121 agaaatgccg atctcaacag tttcctgatt tggtgggaaa acaacggtgc atcacagtcc
      181 atagctactc ccgaaaatca gaattcattc cggattatga ccattcacaa atcaaaggga
      241 cttgatttta aggttgttat catgcccttc tgcgactggt ttctggatgc tgccaaaaat
      301 ccaccgctgc tctggtgtca ggccgatacc gagccgttca acgaatttcc gttgctgccc
      361 atcgattatt cgtccaggct ggtgaattcc attttccaga aggattattt caatgaacaa
      421 atgcatcaat atatcgacaa cctgaatatg gcttacgtgg cttttacacg ggcccgcaac
      481 gaattgctct gctttgcgcc gcagccgaaa aatgaagttc cgatagaaat tgcaaaaatg
      541 gcttccttat cacagcttat tcttaatggc atttcgttgg atcaaaccgc tgataccaat
      601 caaatttcgt tggccgaaaa ctattctccc gaaacaaata gctttgaaat tggcaaaccc
      661 tgcaatgctg tatatactgt aaaaccggca gaaaatgaac tgcggcaaat gaatgactac
      721 ccgatagctg acagccgcaa ccgtttgaaa ataaaacaca gcagccggga ttactggctt
      781 gccgggcaaa cgatggaaga aagtcgcctt aattacggca ccatcatgca cgatatattg
      841 aaaaaaatag ttacacgcag cgatgaagat aaagctatca atgaaatgat agtttcaggc
      901 aaaattacgg agctggaaag tgaaatgatc cgcacagaaa tgaccaaatt ctggaatatg
      961 cccgaaacag cagcctggtt tgacccgctt gcaaagatac tcaacgaaac aagtatcctg
     1021 cttcctacgg gtgagcaata ccgtccggac cggctcatac tcagggataa caaagcagtg
     1081 attgtagatt ataaattcgg tgatgaaatt atgaagtcac atgcattgca gatagcaaat
     1141 tatgaaaata ttatccggca gatgggctac gaaaccgaga catacctgtg ttatgtcagt
     1201 ttgaataaag tggtaaccct gtcatgataa cctccctaac gcaaaatcag tgataaaaat
     1261 aattattcgt tatacggaaa aattattttt atccaactaa ggacagcttt tatcagacag
     1321 aaaaacaaat caaggatttt cacacattga tttagtccgc ccccggtaat ttcttagaaa
     1381 tattttgcac acagttattg tataaccaaa ataaaatctg tacttttgca gtccaaattc
     1441 agcgtaatct ctgacagaat aaaaatgagg tctgtgaata ttgcgcaatg tatgaactta
     1501 aaaaaaatca ggaaatcatg gatttattaa aagttgctga acaggccttt gctgttgaaa
     1561 cagagaaaac cctgccaaca ttcaaaagcg gtgataccat cacagtagcc tatcgtatca
     1621 aagaaggaaa caaagaacgt attcaggaat accggggtga tgtaattaaa attaccggaa
     1681 gcggagacaa aaaacgtttt accgttcgta aagtttcagg aaatattggt gtagaacgta
     1741 tttttccgat ggactcaccg tttatcgaaa gtatcgccat taacaaacgc ggtaaagttc
     1801 gtcgtgccaa actgtattac ctgcgcaaac tgaccggcaa aaaagcacgt atcaaagaaa
     1861 gacgcgtgta agtatatttt tttcgccctg cgaaataaaa aaagagaaat tcagattgag
     1921 tttctctttt tttattccga taccggctgt tcggttatct ttaaattaaa aaaagcgaaa
     1981 ccacctgtta
//
LOCUS       DAOPZK010000235         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_85639_length_1978_cov_25.081123, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000235 DAOPZK010000000
VERSION     DAOPZK010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85639_length_1978_cov_25.081123"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PKH68_09315"
     CDS             complement(<1..350)
                     /locus_tag="PKH68_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNX13172.1"
                     /translation="MNEPKLTVGILFAPKIEFELFGTFLCNGQEITGKHVIDYDNPED
                     FPLQSVREDMTLLFTPKNEETDFFELKDVVIGINFHWERKENQRFKGSLKIIVENEKL
                     TAINVISVEDYLTSV"
     gene            complement(490..1941)
                     /locus_tag="PKH68_09320"
     CDS             complement(490..1941)
                     /locus_tag="PKH68_09320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNX13173.1"
                     /translation="MKTINCFIPYEEVAQVEQTIQNLKKSPLVDTIYLISTSELPSVE
                     GCEVIVVKSLKSTDAIRKIAERASTEYTLIYTKHTTLQWVNYSIERMVAIANDTSAVM
                     AYADHFNVADGNLTEAPVIGYQLGALRDDFDFGSVLLYRSSALKEAVNRMNVDYDFAG
                     LYDLRLKVSQKGELVHINEYLYYEVENDNRKSGEKIFDYVDPKNRAVQIEMEQACTQH
                     LKDIGGYLKPEFKSIEFSDDSFEFEASVIIPCKNRVKTIENAIVSALNQKTKVPYNVI
                     VVDDNSEDGTVEIIKKYTDNPKLIYIAQDKSYHAIGGNWNAAFHHPKCGKFAIQLDSD
                     DMYYDENTVQKFIDAFYEQNCAMVVGTYMMTNFDMEMIPPGIIDHKEWTPDNGRNNAL
                     RINGLGAPRGFYTPLLREINFPTTKYGEDYAVGLRISREYQIGRIYDVIYYCRRWDDN
                     SDASLDIVKMNGHNTYKDRIRTWELQARIKMNM"
BASE COUNT          517 a          437 c          390 g          634 t
ORIGIN      
        1 acgctggtca gataatcttc caccgaaatc acgttgatgg ccgtcaattt ttcgttctct
       61 acaatgattt tcagtgaacc tttaaaacgc tgattttctt ttcgttccca gtgaaaattg
      121 ataccgataa caacatcttt cagttcaaaa aaatctgttt cttcattttt gggagtgaaa
      181 agtaaagtca tgtcctcccg gacgctctga agtgggaaat cttccggatt atcataatca
      241 attacgtgtt tgccggtgat ttcctgacca ttacataaaa atgtaccgaa taattcaaat
      301 tcaattttcg gtgcgaaaag aattccaacg gttaattttg gttcgttcat ggttgcgtac
      361 aatttcttcg aaaagtcata acctttcgaa gaattgatat attttaaatt aaatacctct
      421 tgctcctgtt gaaaacatct tgttttcacc ctcaaaaagt cccctttggg ggatttaggg
      481 ggcttctgtt tacatattca tttttattct tgcctgcaat tcccaggtgc ggatacggtc
      541 tttgtaggtg ttgtgtccgt tcattttcac aatatccagc gaagcatccg aattatcatc
      601 ccagcggcgg cagtagtaaa taacatcgta aatgcgcccg atctgatatt cgcgactgat
      661 gcgtaatcct acagcataat cttcaccata tttggttgtg gggaagttaa tttcacgcag
      721 taaaggcgta taaaatccac gcggtgcacc taaaccgttg atacgtaatg cattatttcg
      781 tccgttatcg ggtgtccact ccttgtgatc aataatgccg ggagggatca tttccatatc
      841 gaaattggtc atcatgtaag tcccgacaac catggcgcaa ttttgctcgt aaaatgcatc
      901 gataaatttc tgaaccgtat tttcgtcgta atacatatca tcgctgtcca gctgaatggc
      961 aaattttcca catttcggat ggtgaaaagc tgcattccag ttgcctccga tggcgtgata
     1021 ggatttgtcc tgtgcgatat agatcagttt tggattatcg gtgtattttt tgattatttc
     1081 caccgtgccg tcttccgaat tatcatccac cacaattaca ttgtacggaa cttttgtttt
     1141 ttgattaagg gccgaaacga tggcattttc gatggttttc acgcggtttt tacacggaat
     1201 tatcaccgat gcctcgaatt caaaactgtc gtcggaaaat tcgatacttt taaattccgg
     1261 tttcaaatac ccgccgatat ctttcaaatg ctgggtacaa gcctgctcca tttcaatctg
     1321 aacagcccgg tttttcgggt ccacatagtc aaaaatcttt tcaccgcttt tacggttgtc
     1381 attttccact tcgtagtaaa gatattcgtt tatatgtacc agttcgcctt tttgtgaaac
     1441 tttgaggcgc aagtcgtaca aacctgcaaa atcgtaatcc acattcatcc ggtttacggc
     1501 ttctttcagt gccgaactgc ggtacagcaa gaccgaacca aaatcaaaat catctctcag
     1561 ggcacccagt tgataaccga tcacaggtgc ttcggtgaga ttgccatcgg ccacattgaa
     1621 gtggtcggca taagccatca cagcacttgt gtcgttggca atagcaacca tccgttcgat
     1681 ggagtaattt acccattgca gcgtggtatg tttggtgtaa atcaatgtgt attccgtgct
     1741 tgcgcgttcg gcaatttttc ggatggcatc cgtcgatttc agacttttca caacaatgac
     1801 ctcacatccc tcaacagaag gaagttcgga tgttgaaatt aaataaatgg tatcaaccaa
     1861 cggtgatttt ttcaggtttt gaatggtctg ctccacctga gcaacttcct cgtaaggaat
     1921 aaagcaattt attgttttca taggcccctc caaacctccc cgaaggggag gctttttt
//
LOCUS       DAOPZK010000236         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_86750_length_1961_cov_23.989507, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000236 DAOPZK010000000
VERSION     DAOPZK010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86750_length_1961_cov_23.989507"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1699
                     /locus_tag="PKH68_09325"
     CDS             56..1699
                     /locus_tag="PKH68_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNX13174.1"
                     /translation="MVSVDGLAVEFGGTTLFSDVSFVINPKDRIALMGKNGAGKSTLL
                     KILAGVRQPSKGKVSTPKDFTIAYLPQHIKTDDSLTVFEETARAFAQLHELENRIAYL
                     NEQLTIRTDYESDEYMQLIEEVSALSEKFYSIEETNIDEHIEKTLFGLGFEREDFNRN
                     TAEFSGGWRMRIELAKILLQNPDVILLDEPTNHLDIDSVQWFEDFLINTAKAVVLISH
                     DRAFVDNVTTRTIEVTMGRIYDYKATYSHYLELRKERREQQQKQFEEQQKMIAETQEF
                     IERFKGTYSKTLQVQSRVKMLEKLEIIEVDEQDTSALRLKFPPSPRSGTYPVVAEGVG
                     KVYGEKRIFSNANYTIERGEKVAFVGRNGEGKSTMVKCIMGEIEHEGTLTLGHNVKIG
                     YFAQNQASLLDEELTVFQTIDDIAVGDIRTKIRDILGAFMFGRDDVDKKVKILSGGER
                     TRLAMIKLLLEPVNLLILDEPTNHLDMRTKDILKQALIEFDGTLIVVSHDRDFLDGLV
                     TKVYEFGHQKVTEHLEDIYGFLAKKKMENLREIESKTNK"
BASE COUNT          581 a          446 c          459 g          475 t
ORIGIN      
        1 tttcgcaaaa taaccgctat ctttgcagcc ttatttcaaa aataatattg attgtatggt
       61 ttcagtggat ggattggcgg tagagtttgg cgggacaact cttttttcgg acgtttcttt
      121 tgtaattaat cccaaagacc ggatagccct catgggcaaa aacggtgcag gtaaatcgac
      181 cttactcaaa attctggccg gtgttcgaca gccttccaaa ggcaaagttt caactcccaa
      241 agattttacc attgcctacc ttcctcagca catcaaaaca gacgacagcc tcacggtatt
      301 tgaagaaact gcccgtgctt ttgcacaatt gcacgaactc gaaaaccgta ttgcctacct
      361 caacgagcaa cttactatcc gtaccgatta tgagtcggat gagtacatgc agctgattga
      421 ggaagtttcg gcgctgagcg aaaaatttta cagcatcgag gaaaccaaca tcgacgaaca
      481 cattgaaaaa acgctcttcg ggcttggttt cgagcgcgaa gatttcaacc gcaatacggc
      541 cgaattcagt ggcggctggc gcatgcgcat cgaactggcc aaaatcctgc ttcaaaatcc
      601 cgatgtaatc ctgcttgatg agcccaccaa tcacctggac atcgattcgg tgcagtggtt
      661 cgaggatttt ctgatcaaca cggctaaggc agttgtgctt atctcgcacg accgtgcttt
      721 tgtggataat gttaccaccc gcaccattga ggttacgatg ggacggattt atgactacaa
      781 agcgacgtac tcccattatc tggaactccg caaggagcgc cgcgagcagc agcaaaagca
      841 gttcgaagag cagcaaaaaa tgatagccga aacgcaggaa tttatcgagc gtttcaaagg
      901 cacttattcc aaaacactcc aggtgcagtc acgcgtgaaa atgctggaaa aactggaaat
      961 tatcgaagtt gacgagcagg acacctcagc cctgcggctg aaattcccgc catcgccccg
     1021 ctctgggacg tatccggtgg tagcggaagg agtcgggaag gtttacggcg aaaaacggat
     1081 tttctccaat gccaattata ccatcgaacg gggagaaaaa gtggcattcg tgggtcggaa
     1141 tggcgaagga aaatcgacca tggtcaaatg tatcatgggc gaaatagagc acgaaggaac
     1201 gcttacactc gggcacaacg tgaaaatcgg ctattttgcc caaaatcagg cttccttgct
     1261 ggacgaggaa ctcactgttt tccagactat cgatgatata gcggtgggcg atatccgcac
     1321 caaaatccgc gatatacttg gtgcatttat gtttggccgc gacgatgtgg ataaaaaagt
     1381 gaaaatcctt tcgggaggcg aacgaacccg cctggctatg ataaaactgc tgctcgaacc
     1441 ggtaaacctg ctgattctcg acgaacccac caaccacctg gatatgcgta ccaaggatat
     1501 tctgaaacag gcactgattg aatttgacgg cacgctcatt gtggtgtcgc acgaccgcga
     1561 ttttctcgac gggcttgtta ccaaagtgta tgaattcgga caccagaaag taaccgaaca
     1621 cctcgaagac atttacggct tcctggccaa aaagaaaatg gaaaatctga gagaaatcga
     1681 aagtaaaacc aataaatgat catttacata gctgtatcag cagtgaatgt cattacccaa
     1741 aacactcgat aagtgaaagc gcccatgata attgaattca tgggcggtgt ttttttacag
     1801 tcggaaacta cccgccttgt gttgaaagtc ttataattat accacttaaa ttcaaacctg
     1861 atgttgttaa tactggattt tagctatgca ggaaatgtat gttgtcggaa tataaatgct
     1921 tgtcagtata aaaaatggtc aaattggtgc aaatgatgtg c
//
LOCUS       DAOPZK010000237         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_86834_length_1960_cov_23.467717, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000237 DAOPZK010000000
VERSION     DAOPZK010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86834_length_1960_cov_23.467717"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /gene="coaBC"
                     /locus_tag="PKH68_09330"
     CDS             <1..1035
                     /gene="coaBC"
                     /locus_tag="PKH68_09330"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNX13175.1"
                     /translation="RNPILVDFFDPTNGNWNSHVDLGLWADLFLIAPATANTMAKMAN
                     GIADNLLLTSYLSARAPVFVAPAMDLDMFSHPATQSNINKLKELGCVIIEPASGELAS
                     GLEGKGRMEEPEKIVQFITDYFSPKILQGKKILITAGPTYENIDPVRFIGNYSTGKMG
                     FALAEACAAGGAEVTLIAGPVKLKTVNSRITRIDVTSADEMYAEATMHFPLMDGAILS
                     AAVADFKPAHQSENKLKRGKDNLVVEFVPNPDIARKLGEMKSDIQFLVGFALETNDEE
                     LNARDKMERKNLDLIVLNSLNDKQAGFGFDTNKVTILGRNGTKNEYELKSKTEVARDI
                     VDEIARLLAK"
     gene            1045..1938
                     /locus_tag="PKH68_09335"
     CDS             1045..1938
                     /locus_tag="PKH68_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNX13176.1"
                     /translation="MKRNLLIISFIVLGVALMAQELNCNLQINSDKVQGSNKEVFKTL
                     QKEANEFINNRRWTDLTYAEEERIDCNINIIISSAEGDTYRSELTVQSRRPVFNSSYF
                     SSLLNFKDNSFIFTYKEFDPLEFSPTSLQSNLTAVLAYYAYLIIGYDMDSYSRLGGTP
                     YFQAAENIVTQAQSSDWAGWKAFENDKNRYALINNIMDEAFKKYRNYFYEYHRLGLDE
                     MTINATNGRAKIAEGLPLLREAKRARPSAVVISAFLDAKNDELINVFAEGSQKEKETA
                     VEILTDINPTAANRYEQIFKK"
BASE COUNT          586 a          454 c          445 g          475 t
ORIGIN      
        1 cgaaatccga tactggttga tttttttgac cctacaaacg gcaactggaa tagtcacgtt
       61 gaccttgggc tgtgggccga tctttttctt atagctcccg ccactgccaa taccatggcc
      121 aaaatggcca acggaatagc cgataatctg ctgcttacat cgtatctgtc agcccgtgca
      181 ccggtttttg tagctccggc tatggatctg gatatgtttt ctcatcctgc tactcaatcc
      241 aacatcaaca aactgaaaga attgggttgc gtaatcattg aacctgcaag cggtgagctg
      301 gccagcggcc tcgaaggcaa aggccggatg gaagaacccg aaaagatagt ccaatttatc
      361 accgattatt tttcgccaaa aatacttcaa ggtaaaaaga tactcattac agcaggtcct
      421 acatacgaaa acatcgatcc ggtacgtttt attggaaatt attctaccgg taaaatgggt
      481 tttgcactgg ctgaggcttg tgctgccggt ggcgctgagg ttacactcat tgccggacct
      541 gttaaactca aaacggttaa ttcccgtatt acccgcatag atgttacatc ggccgatgaa
      601 atgtatgctg aagctaccat gcattttcct ttgatggatg gtgccattct gagtgccgca
      661 gtcgccgatt tcaaaccggc tcatcagtcg gaaaacaagc ttaaacgcgg aaaggacaat
      721 ctggtggttg aatttgttcc caatcccgat atagcccgta agctgggtga aatgaagagt
      781 gatattcagt ttctggtggg atttgcactc gaaaccaacg acgaagaact gaatgcacgg
      841 gataaaatgg agcggaaaaa tctggacctc attgtgctca actcactgaa tgacaaacaa
      901 gccggatttg gatttgacac caataaagtg acgattttgg gcagaaatgg aactaaaaac
      961 gaatacgaac tgaaaagcaa aacggaagtt gcccgcgata tagtggacga aatagcacgt
     1021 ttgcttgcca aataaaaaaa ataaatgaaa cgcaacctgt tgataataag ttttatagta
     1081 ctcggcgtgg cattgatggc ccaggaattg aactgcaacc tgcagataaa ttcggataaa
     1141 gtacagggtt caaacaagga agttttcaaa acactccaaa aagaggcaaa cgaatttatc
     1201 aataaccgcc ggtggaccga tctgacttac gccgaagaag agcgtattga ttgcaacatc
     1261 aatatcatca tcagttctgc tgaaggtgat acataccgga gcgaactcac cgtgcagtcg
     1321 cggcggccgg tgttcaattc ttcctacttt tcgtcactcc tgaatttcaa ggacaacagt
     1381 tttatattta cctacaagga atttgatccg ctggagtttt cgcccaccag cctgcaatcc
     1441 aacctcactg ccgttctggc atattatgcc tacctgatta tcggctatga tatggattcg
     1501 tattcacgtc tgggaggaac accctatttt caggcagccg aaaatattgt tacgcaggcg
     1561 cagagttcag actgggcagg ctggaaggca tttgagaatg ataagaaccg ctatgcactc
     1621 atcaacaaca tcatggatga agcatttaag aaataccgga actattttta cgaataccac
     1681 cgcctcggac tggacgagat gacgataaat gccactaacg ggcgtgcaaa aatagccgaa
     1741 ggactgcccc tgttgcgcga agccaaacgg gcccggccat cggctgtggt tatcagcgct
     1801 ttcctcgatg cgaaaaatga tgaactgatt aatgtcttcg cagaaggaag ccaaaaggag
     1861 aaagaaacag ccgtagaaat cctcaccgac atcaatccta cagccgctaa ccggtatgag
     1921 cagattttta aaaaataaag cccccctgcc ccctcccgca
//
LOCUS       DAOPZK010000238         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_87411_length_1952_cov_24.236162, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000238 DAOPZK010000000
VERSION     DAOPZK010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87411_length_1952_cov_24.236162"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKH68_09340"
     CDS             complement(<1..499)
                     /locus_tag="PKH68_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNX13177.1"
                     /translation="MEDEIIGSNENQDIPTEEGSTPPGFPKTQHLTGMYKNWFLDYAS
                     YVNLDRAVPDIRDGLKPVQRRILHSLKELDDGRFNKVANIIGNTMKYHPHGDASIGDA
                     LVILGQKDLLVETQGNWGNILTGDRAAAPRYIEARLSKFALEVAFNPKTTVWQSSYDG
                     RNREPV"
     gene            complement(513..>1952)
                     /locus_tag="PKH68_09345"
     CDS             complement(513..>1952)
                     /locus_tag="PKH68_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13178.1"
                     /translation="LALFDPDTTITCYMVPVKSNKSDNLSVDSCWFESPVHLLEQKVK
                     LFVSVKNNAAIIAEKVPLKLTINGTQKAVSSLDIAPGSTANTIITYVDKEPGIHHGLI
                     EITDSPVTFDDKFYFSYEIKNSVPVLCINGLQSNVFLNSLMARDSAFSFNNVSVNQVD
                     YNSLASYNLIILNEIPEVSSGLSEELQKFVDNGGHLLIAPAEKMDIRNYQSFLTMLNS
                     NYYTVLNKLPQKIVEINLQHPVYSDVFESVPQNIDLPQVVSYFSMSRLSRSAQDYLMK
                     LQNGEPFLSVQDCGSGKVFLLSVPLNAEFSNFPKHALFVPTIYKIAMMSRMQARLYYV
                     IGSQQPVEVPSVMNGEKDMVYKLKNIGGEDEIIPQMQNRNGRITLILNEQVRTHGNFL
                     LSNKGPVAGLAFNYSKEESDMKCFVPDEITSFIDKNAMKNTMMLEGKAKSLAAVVSEL
                     NQGIKLWKTFVLLALLFLASEVVLLRLWK"
BASE COUNT          513 a          421 c          449 g          569 t
ORIGIN      
        1 taacgggttc gcggtttcgt ccgtcgtaag atgactgcca gacggtggtt ttggggttaa
       61 aagccacttc gagggcgaat tttgagaggc gtgcctcgat ataacgcggt gctgcggcgc
      121 ggtcaccggt gaggatattg ccccagtttc cctgagtttc caccagcaga tctttctgcc
      181 cgagaattac caatgcatct ccaatggagg catcaccgtg ggggtgatat ttcatggtat
      241 ttccgatgat gttggctact ttgttgaaac gcccgtcatc cagctctttg agtgaatgca
      301 atatgcgacg ctgcacgggt ttaagcccgt cgcggatatc aggcacggcg cggtcgaggt
      361 ttacatatga tgcgtaatcg agaaaccagt ttttgtacat acccgtaagg tgctgcgttt
      421 tggggaaccc tggcggggtg ctgccttcct ctgtggggat gtcctgattt tcgttgctgc
      481 ctataatttc gtcttccata atttactgcc tgtcatttcc ataaacgcaa aagtacaact
      541 tctgaagcaa gaaaaagtaa ggccagcaac acaaaagttt tccacaattt tattccctga
      601 ttcagttcgc tcacgactgc cgcaagggat ttggcttttc cttccagcat catggtgttt
      661 ttcattgcat tcttatcaat aaaagaggta atctcatcgg gaacaaaaca cttcatatca
      721 gactcttctt tgctgtaatt aaaggccaga cctgcaacag gccctttgtt ggaaaggagg
      781 aaattgccat gagtacgtac ctgctcattc aaaattaaag tgatgcgtcc attccggttt
      841 tgcatttgag ggattatctc atcttcgccc ccgatatttt tcagcttata aaccatatct
      901 ttttcaccat tcatgacgga aggcacctcc accggctgct ggctgccaat aacataataa
      961 agccgggcct gcatcctgct catcatagct attttataga tagttggcac aaacagagca
     1021 tgtttgggaa aattgctaaa ttcggcatta agaggcacag ataacaggaa aacttttccg
     1081 ctgccgcaat cctgaacgct taaaaatggc tccccattct gtagtttcat caggtaatcc
     1141 tgggctgacc tgctaagacg gctcatcgaa aaatatgaga caacctgtgg aaggtcaata
     1201 ttttggggca cgctttcgaa gacatcagaa tatacaggat gctgcaaatt tatttcaaca
     1261 attttttgtg gcagcttatt gagcacagta taataatttg aattcagcat ggtcagaaaa
     1321 ctttggtaat tgcggatatc cattttttca gctggcgcta tcagcaaatg cccgccattg
     1381 tccacaaatt tttgcaattc ttcggacaag cccgaagaca cttcggggat ttcgttgaga
     1441 atgattaggt tataggaagc cagggagtta taatccacct gatttactga tacgttatta
     1501 aagctgaagg ccgaatccct tgccatgagt gagttcagaa aaacattgga ttgcagtccg
     1561 ttaatacata gtacagggac agaatttttt atttcgtacg aaaaataaaa cttatcgtca
     1621 aaagtaaccg ggctatcggt tatttcaata aggccgtgat gaatgccagg ttctttatct
     1681 acataggtaa tgatagtgtt tgctgtgctt cccggagcaa tatcaaggct gctgacagct
     1741 ttttgcgtac cgttgatagt tagtttgagc ggcacttttt cggcaataat agcggcatta
     1801 tttttcacag aaacaaagag ttttactttt tgctccaaca agtgcacagg cgactcgaac
     1861 cagcatgaat cgacgctcag gttgtcggat ttatttgatt ttaccggtac catgtagcag
     1921 gttatggtgg tatcggggtc gaacagggcg ag
//
LOCUS       DAOPZK010000239         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_89851_length_1918_cov_22.368760, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000239 DAOPZK010000000
VERSION     DAOPZK010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89851_length_1918_cov_22.368760"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKH68_09350"
     CDS             complement(<1..637)
                     /locus_tag="PKH68_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNX13179.1"
                     /translation="MRNKILLLFIFISSISFAQIKTEEPPRLVVGIKVDGMQTSHIQK
                     LWKYFTAGGFRKIISEGTILEQMEHPIVSAGNSSDVATILTGTVPFYHGISGDYYYDR
                     GDKEIKSMLYDEDQVGIGTREKLSAHNLLTTTLTDELMLTSPQSQVHSIAINPEDAIM
                     MGGHTATSVTWIDDVQNKWVTSGYYTKGLSRWADQMNVNGGFRSISSADWTP"
     gene            complement(705..1076)
                     /locus_tag="PKH68_09355"
     CDS             complement(705..1076)
                     /locus_tag="PKH68_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13180.1"
                     /translation="MIVITSCDPVIGYRYTVNNKSDKELKVYFQVHNSDSTTFIQPRT
                     KVDIMEIETWGSNPHDEKDNFLIVTFDSINIEAVDNTKLEVDFRKRENWIYEVDPGKM
                     FLINTGLNIYEIEINNEDFSQ"
     gene            complement(1156..>1918)
                     /gene="hisB"
                     /locus_tag="PKH68_09360"
     CDS             complement(1156..>1918)
                     /gene="hisB"
                     /locus_tag="PKH68_09360"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNX13181.1"
                     /translation="DIAGSYVIGDRATDVELARNLGCKAIYLSNDTETLVEKGLTEYC
                     ALQTTEWDRITEFLFAGERTAVVHRATKETDVLVEFNLDGSGKSEIDTGLRFFDHMLD
                     QIGKHSGCDLKIRVKGDLEVDEHHTIEDTAIALGEALLKALGDKRGIERYGFTLPMDD
                     CLCSTALDFGGRAWLVMDADFKREYVGDFPTEMLLHFFKSLSDAARMNLNIKAYGENE
                     HHKMEGIFKSFAKAIKMAVKRDIYKYELPSTKGVL"
BASE COUNT          525 a          418 c          368 g          607 t
ORIGIN      
        1 aaggtgtcca gtcggccgaa ctgatggatc tgaaaccacc gttcacattc atctgatctg
       61 cccagcgcga aagtcctttg gtgtaatagc ccgaagtgac ccatttgttc tgcacatcgt
      121 caatccatgt cactgaggtg gctgtatgac cacccatcat gatggcatct tcgggattga
      181 tggcaatgct gtgaacctgt gattgcggac tggtaagcat caattcgtca gtaagggtcg
      241 tagtaagcag gttgtgagcc gataactttt cacgtgttcc aattcctacc tgatcttcat
      301 cgtaaagcat tgattttatt tccttatcac ccctgtcgta ataataatca ccagaaatgc
      361 cgtgataaaa aggtactgta cccgtcagta ttgtagctac atcggacgag ttacctgctg
      421 atacgatagg atgctccatc tgttccaaaa tggttccttc cgaaattatt ttacgaaaac
      481 cgcctgcagt gaagtatttc cacaatttct gaatgtggct ggtttgcatt ccgtccactt
      541 taatgccaac caccaatcgg ggcggttctt ccgtttttat ttgtgcaaaa gatattgaac
      601 taatgaatat gaaaagcaat aaaatcttat ttcgcattga taaaaaaatt tcagttatta
      661 atggattgag aatagtgtag tattaaactt atttttcaga atgactattg tgaaaaatct
      721 tcgttgttaa tttctatttc ataaatattt aaccctgtat taatcaaaaa cattttaccc
      781 ggatcaacct cataaatcca gttttcccgt ttccgaaaat ctacttccaa tttggtatta
      841 tcaacagctt caatatttat actgtcgaaa gttacaatca ggaaattatc tttttcgtcg
      901 tgcggattgc tgccccaggt ttctatttcc ataatatcaa cctttgtgcg tggctgaatg
      961 aaagttgttg aatctgaatt atgaacctga aaatacacct ttaattcttt atcagatttg
     1021 ttattgacgg tgtatctgta accaattacc ggatcacagg aagtaatgac aatcattaat
     1081 aaagcaataa ttgaaattac taacagagag gatgattttt ggaaatttgt gtgtaccatc
     1141 agtttgaaat tatttctaca aaaccccctt tgtacttggc agttcatact tataaatatc
     1201 gcgtttgacg gccattttta ttgcttttgc aaaggatttg aaaattcctt ccattttatg
     1261 atgttcattt tctccgtatg ctttgatatt gaggttcata cgtgctgcat cgctcagcga
     1321 tttgaagaag tgaagaagca tttctgtggg gaaatcaccc acatattcgc gtttgaagtc
     1381 tgcatccatc accagccagg cacgtccgcc aaagtccagg gctgtggagc acaggcaatc
     1441 atccatcgga agtgtaaacc cataccgctc aataccgcgc ttatcgccaa gtgctttcag
     1501 cagcgcttca cccaatgcta ttgcagtatc ttcaatggtg tgatgttcat ccacttcaag
     1561 gtcacctttc acccggattt tcagatcgca gcccgaatgt tttcctatct gatcgagcat
     1621 gtgatcgaaa aaacgcagac ctgtatctat ttctgatttt ccggagccat ccaggttgaa
     1681 ttccaccaaa acatcggttt ctttggtagc cctgtggact actgcagtgc gttcgcctgc
     1741 aaatagaaac tcagttatac gatcccactc ggtagtttgc aatgcacagt actctgttaa
     1801 acctttttcc acaagtgttt cggtatcatt actcagataa atggctttac atcccaggtt
     1861 tcgggcaagt tccacatcgg ttgcgcggtc gccgatgaca taggagccgg caatgtca
//
LOCUS       DAOPZK010000240         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_93761_length_1867_cov_23.562362, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000240 DAOPZK010000000
VERSION     DAOPZK010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93761_length_1867_cov_23.562362"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1570
                     /locus_tag="PKH68_09365"
     CDS             2..1570
                     /locus_tag="PKH68_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNX13182.1"
                     /translation="MHTELQLILTPEQAEKKELLQKVVSEKLGITSERISLIRTLRKS
                     IDARGVPKINMSVEVFWDEEAPGKFEVHIDYKFVGNKPSVLVVGAGPAGLFAALKLIR
                     LGMKPVLIERGKEARERKVDIAQLNRNKSIDHESNYCFGEGGAGTFSDGKLYTRSKKK
                     GNVQQILDTFYYHGADEHVLYDAHPHIGSDNLPLVVENMRKTILSCGGEFHFGKKLEQ
                     LIIENDRIVGAKMNGGEVLKANALILATGHSARDIYEMLYRQGVELESKGFAMGVRVE
                     HPQSLINNIQYHAKNVSEYLPAASYALVEQINGRGVYSFCMCPGGHIVPAGTNEESIV
                     VNGMSASRRNSPYANSGIVVEIHPEDIPEEFQQYGVLAGLKFQQHVENLAYLNNGGKG
                     FTAPAQRLDDFVRGKLSSDLPECSYLPGLVSSPLHFWLPDIISSRLREAFKMFDRKMH
                     GYLTNKAVVVGVETRSSSPVRIPRNPETLQHIRIHGLFPAGEGSGYSGGITSSAIDGE
                     NCAEKAAEYIRSME"
BASE COUNT          531 a          393 c          442 g          501 t
ORIGIN      
        1 tatgcatacc gaactccaac tcatacttac ccccgaacaa gccgaaaaaa aggaattact
       61 ccaaaaagta gtatccgaaa aactgggcat aacgtccgag cggatcagtt taatccgtac
      121 gttgcgaaaa tcaatcgatg cccgcggtgt gccaaaaatc aatatgtcag tcgaagtttt
      181 ctgggacgag gaagctcccg ggaaatttga agttcacatt gattataaat tcgtaggcaa
      241 taagccatcg gtgctcgtag tgggggccgg tcctgccgga ctttttgccg ccctcaaact
      301 cattcggttg ggcatgaaac cggtattaat tgaacgtggc aaggaagccc gagaacgtaa
      361 agtagatatt gcgcagctca accgcaacaa aagcattgat catgagtcca actactgctt
      421 tggtgaaggt ggagccggta cattttctga tggcaaactt tatacccgat ccaaaaagaa
      481 aggaaatgta cagcaaatac tggatacatt ctattatcac ggtgctgatg aacatgtact
      541 ctacgatgct catccgcata tcgggtccga taatctgcca ctggtagtgg agaatatgcg
      601 aaaaacaatt cttagttgtg gcggtgaatt tcatttcggc aaaaaactgg agcaattgat
      661 catcgaaaac gaccggatag tgggtgctaa aatgaatggt ggagaagtgt tgaaagctaa
      721 tgcattgatt ttggccaccg gacattcggc acgtgatatt tatgaaatgc tttaccgtca
      781 gggtgtggaa ctcgaaagca aaggctttgc catgggcgtg agggtcgagc atcctcagtc
      841 gctgatcaac aatatacaat atcacgctaa aaatgtaagt gaatacctgc ctgcggcttc
      901 gtatgctctc gtcgaacaaa tcaacggacg cggtgtgtat tcattttgta tgtgcccggg
      961 tgggcatata gttccggccg gaactaacga agaaagtatt gttgtcaatg gtatgtcggc
     1021 ttcgaggcgc aattctccct atgccaattc gggcattgtg gtggaaattc atcccgaaga
     1081 tattcccgaa gaatttcagc aatacggcgt actggccggg ttgaaatttc agcaacatgt
     1141 ggaaaatctg gcttatctca acaatggcgg caaaggtttt accgctccgg cgcaacggtt
     1201 ggacgacttt gtgaggggta aactctcatc cgatcttccc gaatgttcgt atttaccagg
     1261 attggtttcg tcgcccctgc acttctggct accggatatt atcagtagtc gcttgcggga
     1321 agcattcaaa atgtttgacc gcaaaatgca tgggtatctc accaacaaag ctgtggtagt
     1381 gggagtggag acacgttcct catcgccggt gcgcatcccg cggaatcctg aaacacttca
     1441 acatattcgt attcacggac ttttccctgc cggtgaaggg tcaggttatt ccggaggcat
     1501 cacctcatcg gccatcgatg gagaaaattg tgccgaaaaa gcagccgaat atatccgctc
     1561 aatggaataa tggagattta tttaacaaaa aagtgttttt ttgaaaaaaa atatattacc
     1621 tttgtagcct cttagaacaa cggttttagg caattttttt ttaaaatatt gaaaaataat
     1681 cccggttgga aattttgttg catggttttt gctaagtaca tttgtaaaat ctgatttccg
     1741 actgattctg ttcatttcat tattctccgg aattatcaga aaaaacagaa ttctttaaga
     1801 gactccgtag ctcagttggt agagcaattg actcttaatc aatgggtcga gggttcgagc
     1861 ccctccg
//
LOCUS       DAOPZK010000241         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_97904_length_1817_cov_24.675936, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000241 DAOPZK010000000
VERSION     DAOPZK010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97904_length_1817_cov_24.675936"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1817)
                     /locus_tag="PKH68_09370"
     CDS             complement(<1..>1817)
                     /locus_tag="PKH68_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNX13183.1"
                     /translation="SMLNIYRASAGSGKTFQLTKNYIFLLFDAFKNSDHPHRRIMAVT
                     FTNKATDEMKSRILMELHSLYRGEKSDYRAELTKKYLQDEANVNLQAGKILIHILHDY
                     SSFSISTIDKFFQQVIRTFAREIGVNGGYNLELDTETTLQQAVDNLFLELSGDDNKQL
                     LDWLTRFAEEQVEQSANWNIQKEIIKLGKEIFKENFQHKAEETSIKLHDKHYLNEYLQ
                     KLRRIKSGFEKKVTDEAETTLHLLEIHGLEPDYFSRKMMHKTLNDLKNGIYEVKSTFQ
                     NYAESPENCYTKAQKPHIKAAIETAFHSGLKSQLDKILVLVQTEIIHYNTANLILKHI
                     NTLGIMSDLAMQIKKITSDQNIMLISDTNLLLNKIIDNSDTPFVYERTGLNINHFMID
                     EFQDTSVLQWKNFKPLISNSVAANNYNMVVGDVKQSIYRWRNSDWKLLDEEVEHDFAT
                     DRLHTESLTTNWRSDKNIIEFNNAFFSQAAKLLQIKLNENLDQVSAADSELEALRHRI
                     IHAYSDVNQQISPKAAEGHVKFEFIAQDENEEGWKKESLNRIPALLEELVDRGYKPSD
                     VAFLVRKNSEEAEIINYLLSYKNSGNAREGYSYDIVGNEG"
BASE COUNT          428 a          383 c          395 g          611 t
ORIGIN      
        1 ccttcgttgc ccacaatgtc gtaactgtaa ccttcacggg cattcccgga atttttgtac
       61 gacaataagt agttgataat ttcggcctct tcagagtttt ttcttactaa aaaagcgaca
      121 tcggatggct tgtaacccct gtctaccagt tcctcgagca atgcagggat tctgttcaga
      181 ctttcttttt tccagccttc ctcattttca tcctgcgcaa taaattcaaa tttcacatga
      241 ccttcggctg ctttgggtga aatttgctgg ttaacatcgc tgtaagcgtg aataattcga
      301 tgtctcagag cttccagctc gctgtcggcc gcgcttacct gatccagatt ttcgttcagt
      361 ttgatttgca gtaacttagc ggcttgggaa aagaaggcgt tgttaaattc aataatattt
      421 ttatcgctgc gccagttggt tgtcaggctt tcggtatgta gacggtcggt tgcgaaatcg
      481 tgctccactt cctcgtcgag cagtttccag tccgagtttc gccaccggta gatgctttgc
      541 ttcacatcgc ccaccaccat attgtagtta ttggctgcaa cactgtttga tatcagtggc
      601 ttgaagtttt tccactgcaa taccgaagtg tcctgaaact catcaatcat aaaatggttg
      661 atgttaagtc cggtgcgctc gtacacaaaa ggtgtatcac tgttgtcgat tatcttgttg
      721 agcagcaggt tggtgtccga tatgagcatg atgttctggt cgctcgtaat tttttttatc
      781 tgcatggcca ggtcggacat gatacccagt gtgttgatat gctttagtat caggttagcc
      841 gtattgtagt ggataatttc cgtctgaact aataccagta ttttatccag ctgtgatttt
      901 aatcccgagt gaaaggcagt ttcaatagca gctttaatgt ggggcttttg cgctttggta
      961 taacagttct cggggctttc ggcataattt tggaacgtgc ttttaacttc ataaatgcca
     1021 tttttcaaat cattcagcgt tttgtgcatc attttccggc tgaaataatc gggttccaac
     1081 ccgtgaattt caagcaaatg caacgttgtc tcagcctcat ctgtaacttt cttttcaaaa
     1141 ccggatttta ttcgccggag tttttgcaga tattcattca gataatgttt gtcgtgaagt
     1201 tttatgctgg tttcttcggc tttgtgctga aagttttctt taaaaatttc cttaccgagt
     1261 ttgatgattt ctttttgaat attccagttt gctgattgtt ccacctgctc ttcggcaaac
     1321 cgagtgagcc agtcgagcaa ctgtttgtta tcatcgcccg agagctccag aaaaagatta
     1381 tccaccgcct gctgcagggt tgtttctgta tccagttcca ggttataacc tccgttcaca
     1441 cctatttcgc gggcaaaagt gcgtatcacc tgctgaaaaa atttatcgat ggtactgatg
     1501 gaaaaggaag aataatcgtg caaaatgtga atcaatattt ttccggcctg aaggtttaca
     1561 tttgcttcat cctgaagata tttttttgta agttcagctc tgtaatccga tttttcaccc
     1621 cggtagaggg agtgaagttc catcagaata cgcgatttca tttcatcggt agctttgttg
     1681 gtaaacgtca ccgccatgat gcgccgatgt ggatggtcgc tgtttttgaa tgcatcaaac
     1741 agcagaaaga tgtaattctt tgtgagctga aaagttttgc ccgaaccggc agaagcacga
     1801 tatatgttta gcatgga
//
LOCUS       DAOPZK010000242         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_99936_length_1794_cov_24.690052, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000242 DAOPZK010000000
VERSION     DAOPZK010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99936_length_1794_cov_24.690052"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..938
                     /gene="lptB"
                     /locus_tag="PKH68_09375"
     CDS             165..938
                     /gene="lptB"
                     /locus_tag="PKH68_09375"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNX13184.1"
                     /translation="MVLRTEHLFKKYGKRTVVNDVSIQVQQGEIVGLLGPNGAGKTTT
                     FYMTTGLVTPNSGKIFLNEQDITKLPVFRRAQKGIGYLAQEASVFRKMTVEDNISSIL
                     EMTKFSKEYQKEKLETLIAEFNLGHVRKNIGDRLSGGERRRTEIARCLAIEPHFIMLD
                     EPFAGVDPIAVQDIQDIVWQLKDKNIGILITDHNVDETLSITDRAYMLFEGKIMFQGT
                     SEELADNPIVREKYLGRDFKLKVKTRVDTVVNPNIEGFI"
     gene            980..>1794
                     /locus_tag="PKH68_09380"
     CDS             980..>1794
                     /locus_tag="PKH68_09380"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNX13185.1"
                     /translation="MNAKLSIIGSGSALPTKSNFPTSQLLELRNKQFLIDCGEGTQIR
                     MRQMGLKNSHLGHIFISHLHGDHCFGLMGLISTFGMLNHTSDIQIHAHPDLEKLMQPQ
                     LKYFCEGLPFQVLFQPFNPSVNEIIYEDRTIQVSSIPLKHRVPCSGFLFEEKTGDRHI
                     IRDMIDFYKVPVWKIGKIKQGEDFTTEEGEIIPNDLLTLPPTPPIRYAYCSDTAYSEK
                     IIPIIEGVDLLYHEATFMEDELYRARATQHSTARQAAEIAKKANVKRLIIGHYS"
BASE COUNT          526 a          424 c          396 g          448 t
ORIGIN      
        1 ttttcttaaa atttcacata ttgacttatc tctctgtgtt aattcattca gaattttgta
       61 cttttgctgc gttaaaaaaa aacagctaaa tggaagcaga aaatatacag gacattgaac
      121 cggtagataa cactatcaca gaagtcgttc aggaaaagac ctccatggta ctccgcacgg
      181 aacatctttt caaaaaatac ggtaaacgaa ccgttgtgaa tgatgtgtcg atccaggtac
      241 agcagggaga aatcgtggga ttactcggtc cgaacggagc gggaaaaacc actacttttt
      301 acatgactac cggactggtt actcccaact cgggcaagat ttttctgaac gagcaggata
      361 ttaccaaact acctgtattt cgccgcgccc aaaaaggcat cggatacctg gctcaggaag
      421 cttccgtttt ccgtaaaatg accgtggagg ataatatcag ctccatattg gaaatgacga
      481 aattcagcaa ggagtatcaa aaagaaaaac ttgaaacgct gattgctgaa tttaatctgg
      541 gacatgtccg caagaatatc ggtgaccggt tgtcgggtgg tgaacgacgc cgtacggaga
      601 tagcccgctg tctggccatt gaaccacatt ttatcatgct cgacgaaccc tttgcgggtg
      661 ttgaccccat tgccgtgcag gacattcagg acatcgtgtg gcagctgaaa gacaaaaaca
      721 tcggcattct catcactgac cataacgtgg acgaaaccct gagcatcacc gaccgtgctt
      781 atatgctttt cgaggggaaa atcatgtttc agggaacttc cgaggaactt gccgacaatc
      841 ctattgtccg cgaaaaatac ctgggacgtg acttcaaact gaaagtaaag acgagagtgg
      901 atacggtagt aaatccgaat attgaaggtt ttatttaatt cgtcccgttg actttcatgt
      961 ttattgatac acctcaggaa tgaacgcaaa actgtcaatt atcgggagcg gctccgcatt
     1021 acccacgaag agcaattttc caacttcgca actgttggaa ctccggaaca agcaattcct
     1081 gattgattgc ggcgagggaa cacagatacg gatgcggcag atggggttga aaaacagtca
     1141 cctcgggcat attttcatct cgcacctgca tggcgatcat tgtttcggtc tgatgggact
     1201 gatatccact ttcggaatgc tcaaccacac ttctgacatt cagattcacg ctcaccccga
     1261 cctggagaaa ctcatgcagc cacaactgaa gtatttctgc gaaggactgc cttttcaggt
     1321 tctgtttcag cctttcaatc cttcggtcaa tgaaattatt tacgaagacc gaaccattca
     1381 ggtttcgtcc atcccgctca aacaccgggt accgtgcagt ggctttcttt ttgaggaaaa
     1441 aaccggcgac cggcatatta tccgcgacat gattgatttt tacaaggttc cggtctggaa
     1501 aatcgggaaa attaaacagg gtgaagattt tacaaccgaa gaaggcgaaa tcattcccaa
     1561 tgacctgctg accctgcctc ccactcctcc catccgttat gcttactgtt cggacacagc
     1621 ttacagcgaa aaaattatcc ccattatcga aggtgtggat ctgctgtacc acgaagccac
     1681 ttttatggaa gatgaattat accgcgccag agccacccag cactccaccg cccggcaggc
     1741 agccgaaata gcaaaaaaag cgaatgtaaa aagattgatt atcggacatt attc
//
LOCUS       DAOPZK010000243         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_100261_length_1790_cov_27.757925, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000243 DAOPZK010000000
VERSION     DAOPZK010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100261_length_1790_cov_27.757925"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PKH68_09385"
     CDS             complement(<1..1259)
                     /locus_tag="PKH68_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNX13186.1"
                     /translation="MKIFTTTQIRQLDQYTIDNEPVASIGLMERAADRILQHFRKDWG
                     MNTEVVILAGPGNNGGDGLALGRMLLQIGYEVQVILLHSGKISADCKINKERLQEYFP
                     DFFTEQNGRFDPPEMTKETVIVDALFGSGLSKPIDGIFKKAIEWINSRPNRVVSVDIP
                     SGLPGEACRTETDCVVEADFTYSLQFPKLAFLFPENEKFVGKWKVIDIQLHPKVISDT
                     DTKYVFVEKADIRPVLKSRTRFSHKGTYGHALIWAGKKGMAGAAVLASKAALRAGAGL
                     VSVHSVDENRIIMQSAVPEAIFVNELTEPERYNSFAFGPGLGTDEQTAEMLFELLKTL
                     RKPCVLDADALNIISSHKNFLEFIPENSILTPHPGEFERLFGPTANSQERMEKASALA
                     QNYDFFVVLKGANTLIATPDGKMYFNST"
     gene            complement(1324..>1790)
                     /locus_tag="PKH68_09390"
     CDS             complement(1324..>1790)
                     /locus_tag="PKH68_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNX13187.1"
                     /translation="RNIYLHHGGLERVFTDGFHTEKTIFGALKHFRSIFLEIPHEKRT
                     EKHLSDVASNSSAKRLNMFLRWMVRKDEQEVDFGLWNGIPASALMLPLDVHTGDVSRA
                     LGLLQRKQNDWRAVEEITGILRTFDAGDPVKYDFALFGMGIEAHPTLPKGGL"
BASE COUNT          449 a          445 c          393 g          503 t
ORIGIN      
        1 gtggagttga aatacatttt tccgtcggga gtggcaatca gtgtatttgc ccctttcagt
       61 actacaaaaa aatcataatt ctgagccaga gcagaggctt tttccattct ttcctgcgag
      121 ttggcggtgg gtccgaacag cctctcaaat tccccgggat gaggggtaag aatgctgttt
      181 tcgggaataa attcaaggaa atttttgtgg ctggaaatga tattgagtgc gtccgcatcg
      241 agcacacagg gtttccggag cgttttcagc agttcaaaaa gcatttcagc cgtttgttcg
      301 tcggtaccca gtccgggacc aaaagcaaac gagttgtagc gttccggctc agtcaattca
      361 ttcacaaaaa tggcttcagg tacagctgat tgcataataa tccggttttc gtccacacta
      421 tgaacgctca ccagccctgc gcctgcacgc aatgccgctt ttgaagcaag cacagcagca
      481 cccgccatac cctttttgcc tgcccaaatc agtgcatgtc catacgttcc tttatgagaa
      541 aaacgggttc tggatttcag caccggccgg atatcggctt tttccacgaa aacatatttc
      601 gtgtctgtat ctgaaatcac tttgggatga agctgaatat caataacttt ccattttccc
      661 acgaattttt cattttcagg gaaaaggaat gccaatttag gaaattgcag ggaataagtg
      721 aaatcggctt ccaccacgca gtcggtttcg gttctacacg cttcacccgg taatccggaa
      781 ggaatatcca ccgaaactac ccggtttggg cggctgttga tccattctat tgcttttttg
      841 aaaattccgt cgatgggttt cgacaatccc gagccaaaaa gtgcatcgac aattacagtc
      901 tcttttgtca tttccggcgg atcaaaccgg ccgttttgtt cggtgaaaaa atcgggaaaa
      961 tattcctgca gcctctcctt gttgattttg caatcagccg aaatttttcc actgtggagc
     1021 aaaatcacct gaacttcgta tccaatctgc agcagcattc gccccagcgc cagcccgtcg
     1081 ccgccattgt ttccgggacc ggccagtatc actacttcgg tattcatacc ccaatctttt
     1141 ctgaaatgtt gaagtattct gtcggcagcg cgctccatca atccgatgga tgcaaccggc
     1201 tcattgtcaa tggtgtattg atcgagttgt cggatttgag ttgtggtaaa tattttcata
     1261 tcggccccct aaccccctaa agggggaata tatatagatt ttaatttgta aacttgcact
     1321 aacctaaagc cctccctttg ggagggttgg gtgggcttca atccccatcc cgaacagtgc
     1381 aaagtcgtat ttcaccggat caccggcatc aaatgtacga agaattccgg taatttcttc
     1441 cactgcccgc caatcgtttt gttttcgttg gagtaagccg agcgcccggc ttacatcgcc
     1501 cgtgtgaaca tcaagcggaa gcatcagggc tgaagccgga atgccattcc ataagccaaa
     1561 atccacttcc tgttcatctt ttctcaccat ccagcgcaga aacatattga gccgtttggc
     1621 cgacgaattg gaagccacat ccgaaaggtg cttttctgtt cgcttttcgt gcggaatttc
     1681 cagaaatata cttctgaagt gttttaatgc cccgaaaatt gttttttcag tgtgaaatcc
     1741 atcggtaaaa acacgctcca gtccgccgtg atgcagataa atatttcgca
//
LOCUS       DAOPZK010000244         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_102319_length_1767_cov_19.671145, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000244 DAOPZK010000000
VERSION     DAOPZK010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102319_length_1767_cov_19.671145"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1449)
                     /locus_tag="PKH68_09395"
     CDS             complement(151..1449)
                     /locus_tag="PKH68_09395"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX13188.1"
                     /translation="MKDILKSIILEFQQRELPVLMKRKLVINSELPIIITLIGARRSG
                     KTYLLYQIMNELIAGGVKREDMIFLNFEDERLLLNTNQLDLILQAYSELYPDKKLSEC
                     WFFFDEIQNVDGWEKFVRRVFDNYSKHIFVTGSNAKLLSTEIADSLRGRTITYTVYPL
                     SFAEFLNFKEVEYNIIHPLKKAKVINHSLQFIKQGGFPELINFENAERIKVLQSYFNT
                     MIFRDIVERYKVNDVQLLKFFIKKLFATIGKPLSINKIYNEIRSMGYKVSNNYLYEFE
                     QYCYAVFLSISVPKFDFSEIKQAKSDKKLYAIDTGLLSSVEFSISENRGKLLENAVLL
                     ELSKAGYEVFYYKGKYECDFIVKNRNSFQAIQVCWDMDNPTTKNREIRALLDVCLLLH
                     LPNAKIITFDQKQLIKADEVEIQLIPFYEWANEEDLNGMI"
BASE COUNT          515 a          363 c          314 g          575 t
ORIGIN      
        1 cgctgaacca tttacccctt cccgatagct atcgggacag gtagttagga aacaagaggg
       61 agtgtgtgtt ttcagacgta atatgttgtg tctctaccaa acaaaaccct gcctgcggca
      121 ggctgtatta atcatcccat cttcaaattt tcaaatcatc ccattcaaat cttcttcgtt
      181 tgcccattcg taaaatggaa taagttgtat ttccacttca tcagccttaa taagttgttt
      241 ttgatcaaaa gtgatgattt tagcattcgg aagatgtaaa agcaggcaga catccagtaa
      301 agcccggatt tctctgtttt tggttgtagg attatccata tcccaacata cctgtatggc
      361 ctgaaatgaa tttcggtttt taactataaa atcgcattca tattttcctt tgtagtaaaa
      421 aacttcgtaa ccggctttcg ataattctaa caaaacagca ttttcgagta actttcctct
      481 gttttcggat atgctgaatt cgactgatga aagtaaaccg gtgtcgatgg catacagttt
      541 tttatccgat ttggcttgtt ttatttccga aaagtcaaat ttaggtactg aaatgctaag
      601 aaaaacagca tagcaatatt gttcaaactc atataaataa ttattgctta ccttatagcc
      661 cattgatcga atttcgttgt aaattttgtt gatagagaga ggttttccga tagttgcaaa
      721 caattttttt ataaaaaact tgagcaactg tacatcgtta actttatatc gctccactat
      781 atctctgaat atcatcgtat tgaaatagct ttgtaaaacc tttatccgtt ctgcattttc
      841 aaaattgatc agttcgggaa agccaccctg ctttataaat tgcaatgaat ggtttattac
      901 tttcgctttt ttaaggggat gaatgatgtt gtattccacc tctttgaaat tcagaaattc
      961 ggcaaaactc aacggatata cggtgtaggt aattgttcgg cctctgagtg aatcggctat
     1021 ttcggtactc agcagtttag cattcgatcc ggtaacgaaa atatgtttgc tgtaattatc
     1081 aaaaacccga cgaacaaatt tttcccatcc gtccacattt tgaatttcgt cgaaaaagaa
     1141 ccagcactcg cttaatttct tgtcgggata taactcagaa taagcctgaa gtattaaatc
     1201 cagctgattg gtgtttaaaa gtaagcgttc atcctcgaaa ttcaaaaaaa tcatatcctc
     1261 acgttttact ccccctgcta tcaattcgtt catgatctga tacagtaaat aggttttccc
     1321 gctacggcgc gcaccaatca gcgtaattat aataggcaac tctgaattga tcaccagttt
     1381 ccgcttcata agaacgggta attctctttg ctgaaattca agtataattg atttgagtat
     1441 atccttcatt gtatgacaaa tagtttaatc cgactgcaaa gataaaattt ttttcgcata
     1501 attagcgaaa caattaattt ttttattagt acattttact aaacaaatag gatgcacctg
     1561 ctggcgcgga tccgccaaag gtctccgaca cgtcggagca ggctggaaaa gttttgtaac
     1621 ctacttgccg caggcaggtc tgtgtcatct tatttatgca cagtgagggc gcctcccgga
     1681 gtgacaccct cactcctccg caatatcctc tatacccgtc gtaatgccat ccccgagctc
     1741 gcaatgcccg ttgtcgacgg gtattaa
//
LOCUS       DAOPZK010000245         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_103709_length_1752_cov_23.945197, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000245 DAOPZK010000000
VERSION     DAOPZK010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103709_length_1752_cov_23.945197"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..>1752
                     /locus_tag="PKH68_09400"
     CDS             359..>1752
                     /locus_tag="PKH68_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNX13189.1"
                     /translation="MKRITVLLALCLIFSGLVQSQTTAPASFGPTPNSRQMKYLRSPM
                     AAFIHFGMNTFAGSDGIEWGNDVKRPASTFNPAKGKVDTDQWVRLLKKAGFTRVIITL
                     KHHDGFCTWPTKITDYNISKSPYMSGNGDLAKELSESCDKYGLDMGIYLSPWDAWEPT
                     YGDSTPGDYNDFYDGQLRELLGGAYGRLNTETGKREIVEIWLDGATGDGVAHQTYDFA
                     RYVSTVRELQPNCLTWMTLGAAQNYSGPEAGFPVDAFWVGNEKGYVNDPVWMKVAVNG
                     TSVSQYNMNGKYMSIPEADVSIRPGWFYHTSQDGSVKTLDYLINSIYFRSVGMGIPLL
                     LNVPPNREGKFHANDSVALMKFGTAISNTFQTNLLTPQMSVSAGAVRGAGFEAANVLD
                     GNYDTYWTMTDGQTTGTITVDLGKDVEMDVIRLQEYIPLGQRISGWKVEVEVYGSWKE
                     FGSGQTIGYQRMIKG"
BASE COUNT          491 a          371 c          399 g          491 t
ORIGIN      
        1 cggctctggg tacagaaaaa agctcaatca gttgattttc aacgattgag ctttttgttt
       61 ttcctatttc caccacactt gcaccaaaca ttttcatgct tttcattgtt cagatgtatt
      121 ctgtgtactt cccggcgtgg tttcattcga cttattttat ttccagatcc ttcaaatctg
      181 aatatttcta cacacaacgt cgattttact gattccattt ttatctatta atcaaaaatg
      241 cgatagctaa cagagtgtaa taaacgctgg taataatcaa tatatgcggg attcaatcca
      301 atagaatggt attctaaaaa aacttattat ctttgtcctg tcaacctaaa aaaatacaat
      361 gaaaagaatt acagttttac ttgccctgtg cctgatattc tcaggattag ttcaatcaca
      421 aactacagct cctgccagtt ttggccccac acccaacagt cgacagatga aatatctgag
      481 gtcgccgatg gctgcattta tccatttcgg aatgaataca tttgccggat ccgatggtat
      541 tgagtggggg aatgatgtca aaagacctgc atccactttt aatcctgcca aaggaaaagt
      601 tgatacagac caatgggtcc gtttgttgaa aaaagcaggt tttacccgtg ttattatcac
      661 gctcaaacat cacgatggtt tttgtacctg gcctacaaaa attaccgatt acaacatttc
      721 taaaagccct tatatgtccg gaaatggtga tttggcaaag gaattgtccg aatcctgtga
      781 taaatatggt ttggatatgg gtatttatct ctcaccctgg gatgcctggg agcctacgta
      841 tggtgacagt actccgggcg attacaacga tttctacgac ggtcagctgc gcgaattgct
      901 cggcggcgca tacggtcgat taaataccga aaccggtaaa cgcgaaatag tggaaatatg
      961 gctcgacggg gctaccggcg acggtgttgc acatcaaacc tatgattttg cccgctatgt
     1021 ttcaacagtt cgcgaacttc aacccaactg ccttacgtgg atgaccctgg gagcagcaca
     1081 aaattattcc ggacccgagg ccggtttccc ggtggatgct ttctgggtgg gcaatgaaaa
     1141 aggatatgtg aatgatccgg tttggatgaa agtggccgtt aacggcacca gtgtaagcca
     1201 gtacaatatg aatggaaagt atatgagcat tcccgaagcc gatgtttcca ttcgtccggg
     1261 atggttttat cacacatcgc aggatggcag cgtgaaaaca ctggattatc ttatcaacag
     1321 tatttatttc cgctctgtgg gtatgggaat tccgcttttg ctcaacgttc cgcctaaccg
     1381 cgaaggaaaa tttcatgcca atgactctgt agctttaatg aaatttggca cagccatttc
     1441 caatacattt caaaccaact tattgacacc tcaaatgtct gtgtcggccg gtgcagtgcg
     1501 tggtgccgga tttgaagctg ctaacgtgct ggatggaaat tacgatacgt actggacgat
     1561 gaccgatggg caaaccacag gaacaattac tgtggatttg ggtaaggatg tggaaatgga
     1621 cgtaatccgt ctgcaggaat atattccact gggacaacgc atcagcggat ggaaagtaga
     1681 agtggaggtg tacggcagct ggaaagagtt tggctccgga cagacgatcg gttaccagcg
     1741 catgataaaa gg
//
LOCUS       DAOPZK010000246         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_104183_length_1747_cov_20.420804, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000246 DAOPZK010000000
VERSION     DAOPZK010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104183_length_1747_cov_20.420804"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1330
                     /locus_tag="PKH68_09405"
     CDS             248..1330
                     /locus_tag="PKH68_09405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNX13190.1"
                     /translation="MVIVSVLVIFIYALLIINFLRGWIALPEFEASGMANEPRVTIIT
                     ACKNEVNNLPYLFQAVNEQSFRNFEFLLVDDGSTDGSYEYAEQVSLNFPELKLLRNSG
                     KGKKEAIKTAVLQSESEIIVTLDADCLPSVDWLNEIVQFYISYPSDLIICPVKMDSDG
                     SFFQEFQQFEFASLIASGAGAAGMDMPILCNGANLAFRRESWLECMDNLHFEVPGGDD
                     IFLLQSIKKRKGIIRFLKSVNATVTTHSAVNLKSFIHQRRRWASKKSAYTDWQLALTA
                     AVVFLASFIVLLDFVLAIVQPNLIALVLNIFLLKLLIDVSFFYKISDFFGLRKVLLNT
                     FVFSLIYPFYIVFTAILSLAGSKKTW"
     gene            complement(1331..>1747)
                     /locus_tag="PKH68_09410"
     CDS             complement(1331..>1747)
                     /locus_tag="PKH68_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13191.1"
                     /translation="LSSCENEDQRRIPEASVYMELNLAASYPTFRNSVNDTLVFTRPR
                     NGHPTDRVGYGGILVYTFVNGTDLNYCAFDLCCPNEVNPNIRVNPNDRGEAVCKSCGT
                     VYQLLTGTGMVISGPSKWNLKRYTVQLSGDMLYISR"
BASE COUNT          466 a          356 c          393 g          532 t
ORIGIN      
        1 gtaaaaacgg tgttgaagaa atcatcgatt ataaattgaa cgatgaagaa aaagcgctgt
       61 ttgcaaaaag tgcagatgct gttcgcacga ccaacgacgt actgaaagaa ctgaatatgt
      121 tgtaatatac aggtctgata aatttataga tcgctgtaag caattctgct tgcagcgatt
      181 ttctttttat gacttcagag tataatcggc tttattttac tatttttgtt ttcatagaat
      241 atgtcgtatg gttattgttt cagtcctggt gatttttata tacgctctgc tgataattaa
      301 ttttctcagg ggctggattg ctttacccga atttgaagct tcaggaatgg cgaatgaacc
      361 ccgtgttacc attattacgg cctgtaaaaa cgaagttaac aacctgccgt atttatttca
      421 ggctgtgaac gaacaaagct ttcgtaattt tgagttccta ctggtggacg atggctccac
      481 cgatggcagc tatgagtacg ctgagcaggt ttcgcttaat tttcccgaat tgaaactact
      541 ccgaaacagc ggaaaaggca aaaaggaagc tatcaaaacg gcagtcctgc agtctgaaag
      601 cgaaatcatc gtaaccctcg atgccgactg ccttccatcg gttgactggc tcaatgaaat
      661 tgtgcaattt tatatctctt atccctccga tctgattatt tgtccggtaa aaatggattc
      721 tgacggcagc ttttttcagg aatttcagca gtttgaattt gccagcctga tagccagtgg
      781 agcaggtgct gcagggatgg atatgccgat attgtgcaac ggggccaatc tggctttcag
      841 gcgcgaaagc tggttggagt gtatggataa tcttcatttt gaagtgcccg gtggtgatga
      901 tattttttta ttgcaaagca ttaaaaaaag gaagggaata atccgctttt tgaaatcggt
      961 aaatgcaacg gtgaccactc attccgctgt caatctgaaa tcattcattc atcaacgccg
     1021 caggtgggct tccaagaaaa gtgcctatac cgactggcaa ctggctttga cggcagcagt
     1081 cgttttttta gcgagtttta ttgttttgct ggattttgtt ctggcaatag ttcagccgaa
     1141 tttaatagca ttagtcctga atatcttttt attgaagctg ctgattgatg tttctttctt
     1201 ttacaaaatc agtgattttt ttggactaag gaaagttttg ctgaatacgt tcgtattttc
     1261 gctcatttat cctttttata tcgtcttcac agctatcctg tcgctcgcag gttccaaaaa
     1321 aacctggtaa ttacctggaa atgtatagca tatcaccgct cagttggacg gtatatcgct
     1381 tcaaattcca tttcgatgga cccgaaatca ccattccggt gccggtaagc aactgataaa
     1441 cggttccaca cgatttgcag accgcttccc ctcggtcatt gggatttacc cgtatgttgg
     1501 ggttcacctc attgggacag cagagatcaa atgcgcagta attcaggtcg gtgccattca
     1561 caaacgtgta caccagaata cctccgtaac caacccgatc agtgggatgt ccgttgcgtg
     1621 gacgggtaaa cacaagtgta tcattcacgg aattgcgaaa agtggggtag gaggcagcca
     1681 gattaagttc catatacacc gaggcctcgg gaattcgacg ctgatcttca ttctcgcagc
     1741 tgctcag
//
LOCUS       DAOPZK010000247         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_105964_length_1728_cov_28.410042, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000247 DAOPZK010000000
VERSION     DAOPZK010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105964_length_1728_cov_28.410042"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..>1728
                     /locus_tag="PKH68_09415"
     CDS             141..>1728
                     /locus_tag="PKH68_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNX13192.1"
                     /translation="MKKHVVLLFLFIFLFSRNIISVSASEPDSAFLFSYSTLKNDGHN
                     GLHFAWSIDQVNWHSIGPEHRFLFSDYGRWGAEKRLVTPFLFQDNKQIWHCLWSLNEY
                     DGTFAHSSSTDLIYWLPQTYPNVIKDNNCLQPEVSFDKASGLYTITWISNLHKDSLVY
                     AATTRDFKEYSATIPLNKDIRLNKRQHVTIAGNTQTGTVHKVSWNVINNLIKAWQLSE
                     YNNNLSNENFKDDSARFASLKPVQLTINANVNQRKKISDMLIGVFFEDINYAADGGLY
                     AELVQNRDFEYSLSDKSGRDKSWTSTKAWNVIGNGVALTIDTVSPIHPNNPHYATLNV
                     SQTGSALINEGFNGIAITKGEKYNFSVFVRKPDNKTGYISIRLTGKNGEICGETRIKA
                     TAGKWQKVKAVITANKTVADASLAIIPETTGKVSLDMISLFPQKTFKGHSNGLRTDLA
                     EAIAGLHPRFVRFPGGCVAHGDGIGNIYRWKNTIGPLESRKPQRNLWNYHQSAGLGYF
                     EYLQFCEDIGAKPVPVIAAGV"
BASE COUNT          519 a          394 c          362 g          453 t
ORIGIN      
        1 taattttggg cgaattatcg gtttctgata ccacgacaaa attattcaaa tgaaacgagc
       61 atgaacacag aggttctcca aagggtatga aaatctgaca tgcgagttcc atgtggcaat
      121 ttaaaaacaa ttatttacac atgaaaaaac acgttgtttt acttttcctg tttatttttc
      181 tgtttagccg gaatattatt tctgtttctg ccagtgagcc tgactctgcc tttctttttt
      241 cgtattcaac cctgaaaaac gacggacata acggattgca ttttgcctgg agcattgatc
      301 aggtcaactg gcattctatc ggacctgaac atcgcttcct tttcagcgat tacggacgct
      361 ggggcgccga aaaaagattg gtaacccctt ttctgtttca ggacaacaaa caaatctggc
      421 actgtctctg gagcctgaat gaatatgatg gtacttttgc acactcctct tccaccgatt
      481 taatatactg gttacctcaa acctatccca acgtcattaa ggataataac tgcctgcagc
      541 cggaagtttc cttcgacaag gcaagcggac tttataccat cacctggatc agcaacctcc
      601 ataaagattc attggtttat gctgccacta cccgcgattt taaggaatac tccgcgacaa
      661 taccgcttaa caaagacatc aggctgaata aacggcaaca tgtaacaatt gctgggaata
      721 cccagaccgg aactgtccac aaagtttcat ggaatgtgat caataacctg ataaaagcct
      781 ggcagttgtc ggagtataac aataacttat caaacgaaaa ttttaaggac gacagcgcac
      841 gatttgcctc tctgaaaccc gtgcagctta ccatcaacgc gaatgtaaat cagcgtaaaa
      901 aaatcagtga tatgttgatc ggagtttttt tcgaggacat caactatgct gctgatggcg
      961 gattatatgc cgaacttgtt cagaatagag actttgaata ctctctgagc gataaaagcg
     1021 gcagggacaa atcatggaca agtacaaaag cgtggaatgt catcggaaac ggtgttgcgc
     1081 ttactatcga tacggtttca cccattcacc ccaacaaccc acactatgcc accctgaatg
     1141 tttcccaaac cggatcggca ttgattaacg agggttttaa cggcattgcc attacaaaag
     1201 gggaaaaata taatttttcg gtttttgtac gtaaacctga caacaaaacc ggatacatat
     1261 ccatccgtct tacgggaaaa aacggggaaa tctgcggaga aaccagaatt aaagcaactg
     1321 cgggaaagtg gcaaaaggtg aaagctgtca tcactgccaa caaaacggta gccgacgcca
     1381 gtctggcaat tataccggaa accacaggaa aagtttcact ggatatgatt tcactgtttc
     1441 ctcagaagac attcaaggga cattctaacg gattgcgtac cgatcttgcc gaagccattg
     1501 ccggattgca tcctcgcttt gttcgttttc ctggcggttg tgtagcacat ggcgacggga
     1561 ttggaaacat ttatcgctgg aaaaatacaa tcggacctct cgaatcacgt aagccacagc
     1621 gcaatctttg gaactaccac cagtccgccg gattgggtta ttttgaatac ctacagttct
     1681 gcgaagatat cggagcaaaa cctgtgcccg taattgctgc gggcgtac
//
LOCUS       DAOPZK010000248         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_107141_length_1716_cov_24.461770, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000248 DAOPZK010000000
VERSION     DAOPZK010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107141_length_1716_cov_24.461770"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PKH68_09420"
     CDS             <1..857
                     /locus_tag="PKH68_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HNX13193.1"
                     /translation="SNLPEDDFIQHEDLPYCIGEFVWTGFDYLGEPTPYYTNWPSHSS
                     LFGIVDLAGIPKDRFYLYRSHWNKEAETLHILPHWNWKGREGQVTPVFVYTNYPSAEL
                     FINGVSQGKRTKDLSIKRDGSDTEMAKKNFDRQKRYRLMWMDTKYQPGTVKVVAYDAN
                     GKAVAEKVMKTAGEPYKIVLEADRSSIAADGKDISFVNVKIVDKDGNFCPTDEREISF
                     KVKGAGVFKAVANGNPSSLESFQTPKMKLFSGQLTALVQSAEKGGIITLEATAKGVKA
                     GKISIEVR"
     gene            1085..>1716
                     /locus_tag="PKH68_09425"
     CDS             1085..>1716
                     /locus_tag="PKH68_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNX13194.1"
                     /translation="MNPYKILVVDDEEDLCEILQFNLETEGYTVDVANSAEEALKKDL
                     TAYNLLLLDVMMGEISGFRMARMLSDDPKTASIPIIFLTAKDTENDRLTGFNIGADDY
                     ISKPFSIREVIARVKAVLRRADTAREPELPAELKHENLVMQLDNKKVVLNGEEIPFTK
                     KEFEILKLFLENKNRVFTREELLLRVWSDEVIVLNRTIDVNITRLRKKIGP"
BASE COUNT          541 a          355 c          385 g          435 t
ORIGIN      
        1 ggtccaacct gcccgaagat gattttatcc agcacgaaga tttgccctac tgtattggcg
       61 aatttgtgtg gacaggattc gattacctgg gcgaacctac tccgtactat accaattggc
      121 ccagtcacag ttcgctgttt ggtatcgttg acttagccgg aattccaaag gatcgtttct
      181 acctctatcg cagccattgg aacaaggaag cggaaacact gcacatcctt ccgcactgga
      241 actggaaagg ccgcgaagga caggttaccc cggtatttgt ttataccaat tatccttcgg
      301 ctgaactctt tatcaacggt gtaagtcagg gaaaacgcac caaagacctt agcatcaaac
      361 gcgatggaag cgataccgaa atggctaaaa agaattttga tcgccagaaa cgctaccgcc
      421 tcatgtggat ggataccaaa tatcaacccg gaacggtgaa agtggtggct tatgatgcta
      481 atggcaaagc ggtagccgaa aaagtgatga aaacagccgg tgaaccctat aaaattgtgc
      541 tggaagccga tagaagcagt attgcagccg atggtaaaga catctccttt gtcaatgtga
      601 aaattgtgga taaggacggc aatttctgtc ccaccgatga acgggaaatc agtttcaaag
      661 ttaaaggtgc gggagtattc aaggcagttg caaatggcaa cccctcgagt ctggagagtt
      721 tccagacacc aaaaatgaaa ctgttcagcg gacagcttac cgcactggtg cagagtgccg
      781 aaaaaggtgg aataatcaca ctcgaagcaa cagccaaagg agtgaaggca ggcaaaattt
      841 ccatcgaagt caggtaaaat taattttcaa gaaaatgaat gaactctcgg ttaggtttca
      901 atgacttatc cgggagtttt tttcttgtac aattttaatt tcaaacgaac aaaaatgtaa
      961 tatatttgta acaattatta cacgtcacag aaatatcaaa actgttattt tgcattgaat
     1021 attgttttca gtaatatttt acttttcaca ttgtttgcaa aatatcccct taaaaaataa
     1081 aagaatgaat ccttacaaaa ttttggttgt tgacgatgaa gaagatctgt gtgaaattct
     1141 tcagtttaat ctggaaacgg aaggttatac cgtcgatgtg gctaattcgg ccgaagaggc
     1201 tctcaaaaaa gacctgacag catataacct tttactgctc gatgtgatga tgggcgagat
     1261 ttccggtttc cgcatggcac gcatgctgag cgacgacccc aaaacggcat caattccaat
     1321 tattttcctt acagccaaag acaccgaaaa cgaccggttg accggattta acatcggcgc
     1381 cgacgattat atctctaaac cgttttcaat tcgggaagtg attgcccgtg tgaaagctgt
     1441 attgcgacgg gctgataccg ccagagagcc tgaactgccc gctgaactga aacatgagaa
     1501 cctggtgatg caactcgaca ataaaaaagt agtgttgaac ggtgaggaaa tacctttcac
     1561 caaaaaggaa tttgaaatac tcaagttgtt tttagaaaat aaaaacaggg tttttacccg
     1621 tgaagaattg ctgctcagag tgtggtccga tgaggttatt gtgctcaaca gaaccatcga
     1681 tgttaacatc acccgcctgc gcaaaaaaat aggacc
//
LOCUS       DAOPZK010000249         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_110544_length_1682_cov_20.308543, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000249 DAOPZK010000000
VERSION     DAOPZK010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110544_length_1682_cov_20.308543"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PKH68_09430"
     CDS             <1..1416
                     /locus_tag="PKH68_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNX13195.1"
                     /translation="YIFDKMVLTVSLAHQYFDDQMNLDQDFSPLSIFTLQQNQKQNML
                     NGEAVLKSVSTGNYNWLIGVNGFGERLNTSAPVSFKEDGIRTILQPVFTKIGQASGMT
                     MTVINPTFDIPGTYITKRGGGAVFHQSTFENLMVEGLSLTLGARLDIEKVTLDYNSSS
                     YLDVQAKRGPVVMGVQRATAELIGTADTTFVEFLPKAALKYEWDRMNFVYGSVSRGYK
                     TGGFNIQMISDLISDKLKNTRPGAPAQETDVKHAVMYQPEQSWNYEAGIQNTFLDRKL
                     RTSLTLFYMDIKGLQLTEFVTSGAGRKLTNAGTAVSKGLEVSADMKLTPELNAGLSYG
                     YAHASFKNYTQVETVNGQSVEVDYSGKFVPYAPQHTANVYLNYTKKLENSFITELYGT
                     LSYSGIGKIYWQDSNEISEDFYSLADAVVGVKKNAFGLELWGKNLLNTSYNAFYFESF
                     GNKFFQQGKPVQVGARLTLEI"
BASE COUNT          519 a          356 c          385 g          422 t
ORIGIN      
        1 tacatttttg acaagatggt attgacggtt tcgctggcac accagtattt cgacgatcag
       61 atgaacctcg atcaggattt ttcaccctta tccatattta ctctgcagca aaatcaaaaa
      121 caaaacatgc tgaatggtga agcggtcttg aaatcagttt caaccggcaa ttacaactgg
      181 ctgattggcg tgaacggttt tggagaaagg ctcaacacat cggctcccgt gagtttcaaa
      241 gaagatggaa ttcgtaccat tcttcagccg gtgtttacca aaatcggaca ggcttcaggc
      301 atgacgatga ctgtgataaa tcccacattt gatattcccg gaacttacat tactaagcgg
      361 ggcggagggg ccgtatttca ccagtctacc ttcgaaaatc taatggtcga aggactgtca
      421 ttaacgcttg gcgcacgttt agacatagaa aaggtgacgc tggattacaa ttcgagttcg
      481 tacctggatg ttcaggctaa aagaggcccc gtagtaatgg gtgtacagcg tgcaacagct
      541 gaattaattg gaactgctga taccactttc gtcgaatttc tgccaaaagc agcattgaaa
      601 tacgaatggg acagaatgaa ttttgtgtac gggagtgtat cgcgcggcta taaaaccggc
      661 ggatttaata ttcagatgat atccgatctg atcagtgaca aacttaaaaa cacccgtccg
      721 ggagctccgg cacaagaaac agatgtgaaa catgccgtca tgtaccagcc ggaacaaagc
      781 tggaactatg aggccggaat tcaaaatacc tttctggata gaaaactgcg tacatcgctc
      841 acgctctttt atatggatat caaaggactt caactcaccg aatttgtaac cagcggtgca
      901 ggaagaaaac tcaccaatgc gggaacggct gtgagcaaag ggctggaggt ttcggccgat
      961 atgaaactga cccccgaatt gaacgccgga ctgagctacg gatatgcgca cgcatcgttc
     1021 aaaaactata cacaggtgga aacagtgaac ggacaatctg ttgaagtgga ttactccggc
     1081 aaatttgtac cctacgcacc gcagcatacc gccaatgtgt acctgaatta caccaagaaa
     1141 ctggaaaact cgtttattac tgaactgtac ggaacactct catacagtgg tattggtaaa
     1201 atttactggc aggattcaaa tgagatatcc gaagatttct acagcctggc cgatgcggta
     1261 gtgggtgtaa agaagaatgc cttcggactc gaattgtggg gtaaaaatct gttgaatact
     1321 tcatataatg ccttctattt tgagtcgttc ggcaataagt ttttccagca gggcaaaccc
     1381 gtgcaggtag gagcaagact tacgctggaa atctgagcat aaaacaaaga caaataaaag
     1441 ccaaccggca atacgtgtga aagcgcgtat tgccggttga ctttttaaat aattcaaccg
     1501 gaacaatttt tacataaatt aaaattttct gttattgaaa tgaagcaaag cggacgactt
     1561 agaacgaatg aaaagttatt ttcttcacgg ttgatttaag atatcatacc gggaaaaact
     1621 aatctgatta cttcttcaaa ctcccataca tctcttcagc agccttgcgg gcggctccgg
     1681 ga
//
LOCUS       DAOPZK010000250         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_115517_length_1636_cov_22.798229, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000250 DAOPZK010000000
VERSION     DAOPZK010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115517_length_1636_cov_22.798229"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PKH68_09435"
     CDS             complement(<1..971)
                     /locus_tag="PKH68_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNX13196.1"
                     /translation="MVSKIETSLPGGVEEVSVFTLSEHTDTTVFYGVNNSNMKILRHL
                     HPNLHIVARGHVIKIYGNEIEVRNLTENLEKVEAYALNNNLLTEENIIEIVKGNQPNE
                     ISFDNLILHGVNGKSIMARTANQQKLVKDFETNDLLFAIGPAGSGKTYTAIALAVRSL
                     KNREIKKIILSRPAVEAGEKLGFLPGDMKEKIDPYLQPLYDALQDMLPAAKLREYMEN
                     GTIQIAPLAFMRGRTLSDAVVILDEAQNSTTMQIKMFLTRMGLNTKIIVTGDTTQIDL
                     PYAQKSGLIDALEVLKNTKGIAKVEFDKGDIVRHKLVQRIVEAYEKR"
     gene            complement(1034..>1636)
                     /gene="glyA"
                     /locus_tag="PKH68_09440"
     CDS             complement(1034..>1636)
                     /gene="glyA"
                     /locus_tag="PKH68_09440"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNX13197.1"
                     /translation="PRGGIIMMGKDFPNPWEKTTPKGELKMMSALLDSAVFPGTQGGP
                     LEHVIAAKAVSFGECLQPEFKEYQTQVQKNAKALANALIKRGFKIVSGGTDNHSMLVD
                     LRSKYPELTGKIAEKVLVEADITCNKNMVPFDSRSAFQTSGIRLGTPAITTRGAKEPL
                     MEEIAEMIETVLSNIDNEEVIKSIKERVNKTMANYPLFAY"
BASE COUNT          407 a          375 c          316 g          538 t
ORIGIN      
        1 ctcctcttct catatgcctc cactatccgt tgcaccagtt tatgacgaac aatatcccct
       61 ttgtcaaatt caaccttagc tattcctttg gtatttttca aaacttccag ggcatcaatc
      121 aaacctgatt tttgtgcgta aggcaggtca atctgagtgg tatcaccggt aactattatc
      181 ttggtattca gtcccatgcg cgtaagaaac atctttatct gcatcgtggt cgaattttgt
      241 gcttcgtcca gtatgaccac agcatcgctc agcgtacgac cacgcataaa tgccagcggt
      301 gcaatctgga tagtgccgtt ttccatatat tctctgagtt tggcggccgg cagcatatcc
      361 tgcagcgcat cgtacaaggg ttgaagatag gggtcaattt tttctttcat atctcccggc
      421 agaaaaccga gcttttcacc tgcttccact gccggccgac tgagaataat cttttttatt
      481 tccctgtttt taagtgaacg aaccgctaac gctatagctg tgtaagtttt tccggatccg
      541 gcaggtccga tggcaaaaag caaatcattg gtttcaaaat ctttcaccag tttttgctga
      601 ttggcagtgc gagccatgat ggactttcca ttgacaccat gtaaaatcag attgtcgaaa
      661 cttatttcgt tcggttggtt tccttttacg atttcaataa tgttctcctc tgtcaacaaa
      721 ttgttattca aagcataagc ctccactttt tcgagatttt cagtgaggtt acgaacttca
      781 atctcgttac cataaatttt tataacatga ccccttgcaa caatatgcag gttcgggtgt
      841 aaatgacgca atattttcat attcgaatta tttacaccat aaaatacggt ggtgtcggta
      901 tgctccgata atgtaaagac cgaaacttcc tcaactcctc ccggtaagga agtttctatc
      961 ttagaaacca tatcatctgt tttttccatt tcacttaaat aagcctcccc tttgcgaagg
     1021 tttggaaagg ctgttaatag gcaaacaacg gataattagc cattgttttg ttcactcttt
     1081 cttttattga ttttattact tcttcgttat caatgtttga aagaacggtt tcaatcattt
     1141 cggcaatttc ttccatcagt ggttcttttg ctccccgggt tgtgatggca ggagttccca
     1201 gccggatacc ggatgtctgg aatgctgagc gactgtcgaa gggaaccatg tttttattgc
     1261 aggtaatatc ggcttccact aatacttttt ctgcaatttt tcctgtcaat tcaggatatt
     1321 tggaacgcaa atctaccagc atggaatgat tgtccgttcc gcccgaaaca attttgaaac
     1381 ctcttttgat cagagcgttt gccagtgctt ttgcgttttt ctgaacctgt gtctgatatt
     1441 ctttaaattc aggttgcaga cattcgccaa aagaaactgc tttagcagca atgacatgtt
     1501 ccagcggtcc gccctgagtg ccgggaaata cggcagaatc cagcagtgcc gacatcattt
     1561 tcagttcgcc tttcggagtt gttttttccc acggatttgg aaaatcctta cccatcataa
     1621 taatacctcc acgagg
//
LOCUS       DAOPZK010000251         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_116106_length_1631_cov_17.155457, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000251 DAOPZK010000000
VERSION     DAOPZK010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116106_length_1631_cov_17.155457"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1004..1603)
                     /locus_tag="PKH68_09445"
     CDS             complement(1004..1603)
                     /locus_tag="PKH68_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNX13198.1"
                     /translation="MFSALILAAGLSERMGRQKALLPWNESTTFLEKLINEYVEAGCN
                     PVVCTVNEQVFSHCKNLEISSGVRLLCNRYPERGRMYSVRLGLEALKERPYCFLQNVD
                     NPFINGKVIRRIAGFADPYAWCSPEYKGQGGHPVLLPKKIIEKLLFVDDEDMTLRTLL
                     SSFPSKTVIMEDDTICLNVNTPEEFAKLRQVIRDGQQRP"
BASE COUNT          495 a          318 c          335 g          483 t
ORIGIN      
        1 tcttcgcggg ggccattttc acgaaagaca agttctcctg tatcgtattg tttttccctg
       61 acactgtgag ccagttcatt aaattcttca tcattcaggt tcctgaagag ttcaattttt
      121 tttaagaaca aaagtgtttt atcgatttcc atagcgtttt ctccctattt tattaccaat
      181 tgaaaaatac tgtaacacaa aggtaattaa aatacaagta attataaaca aacgagggtg
      241 cccaaagcta ctgcgcaccc tcgttcaatt ttaaaatgaa aaggtcattt cataagagaa
      301 gctgcgaaat aggaaatagc agccaaagcg actataaaac caacgaataa taacatgaaa
      361 cgaccgaaag ttcctttatt ttctgaatta tcatttgttc ccattgtttt tgtttttaaa
      421 aattatacag ttccaatggt tgaatccaaa gggacaacca tcgattaata cttgaattag
      481 ggagtagaat taagggaaac gggaatgaaa tcagatttgg aatttgcaaa tctgttcaag
      541 tggttttgaa ttggagagat cagaaacgag aggagtatga tcgattcttt ctgaagctaa
      601 taatgtttta ggtatttcag aataggtatg gctttcagaa taacacttaa gcagtggtaa
      661 aacatgaata ttttggaacc gttcgaagcc tggtaaaaga tagtcagagg cagcggtatt
      721 aatagaggag aactttatgt cactgttgtt tttctcttgt tcaactttgg ttaatacctg
      781 attagaataa cgcgaaaaag taagaaatgc aaagagaatg agggcgacgg aaacgatatg
      841 atattttcga aatagctcca ttttgattgg gatccaagag tttgcaaagg tacaaaattt
      901 cgggaaacca ccggaatctt ttcatcacat gtaaataggt gaattatcag aattatggca
      961 aattatcgtg aattctgaaa acacaggtcc gcctcctgaa gggttatggg cgttgttgcc
     1021 catcacggat cacctggcga agttttgcaa attcctcagg agtgttgaca ttcaggcaga
     1081 tggtatcgtc ctccatgatc accgttttgg atggaaatga tgacagtaaa gttctcaggg
     1141 tcatatcttc atcatcaaca aacagcaatt tttcaatgat cttcttcggc agcagcaccg
     1201 gatgaccgcc ctgccctttg tattccggtg aacaccacgc gtagggatca gcaaatcctg
     1261 caattcttcg gatgaccttt ccattgatga aaggattatc aacattctgc aagaagcagt
     1321 agggtctctc tttcagcgct tccagcccca gcctgaccga gtacatcctt ccacgttccg
     1381 ggtaccgatt gcataatagt ctcaccccgg atgagatttc gaggttttta caatgggaaa
     1441 acacctgctc attgacggta caaacgaccg gattgcatcc tgcttcaaca tactcgttga
     1501 taagcttttc aaggaaggtg gtcgattcat tccacggaag aagcgctttc tgacgcccca
     1561 ttctctctga taaacctgct gcaaggatca gtgcggagaa catttgtatt ctgttttatc
     1621 gattctcttt c
//
LOCUS       DAOPZK010000252         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_118868_length_1607_cov_21.389820, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000252 DAOPZK010000000
VERSION     DAOPZK010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118868_length_1607_cov_21.389820"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="PKH68_09450"
     CDS             <1..1496
                     /locus_tag="PKH68_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13199.1"
                     /translation="GTSKIAYVISDFQKSTSQLALFDPDTTISCYMVPVKSNKSDNLS
                     IDTCWFESPVHLAGQKVKLFVSVKNNADIMAEKVPLKLTINNTQKAVSSFDIAPGSSA
                     GTIITYVDKEPGIHHGVIEISDSPVTFDDKFYFSYDIQTSVPVLCINGQQSNVFLNSL
                     LAEDSAFSFHNVSVNQVDYNSLASYNLIIINEIPAVSSGLSEELHKFVNSGGHLLVVP
                     GENMEIGNYQSFLTVVNSNYYTTLNKLPQKIAEINLQHPIYSDVFESVPQNIDLPQVS
                     SYFSMSRLSRSAQDYLMKLQNGEPFLSVQDCGSGKVFLLSVPLRAEYSNFPKHALFVP
                     TIYKIAMMSRMQSRLYYIIGRDQQVEVPTMLNDEKDMVLKLKNTAGNDEVIPQLQNYN
                     GHITLVLNDQVRNHGNYILSNNGPVTGLAFNYSKDESDMKCYSPEEITAFIDKNNLKN
                     TKLLEGKAKSLSVVVSELNQGIKLWKTFVLLALLFLALEVVLLRLWK"
BASE COUNT          499 a          336 c          328 g          444 t
ORIGIN      
        1 gcggcaccag caaaatagcc tatgtaattt ccgattttca aaaatcaacc tcacagctcg
       61 ccctgttcga ccccgatacc accataagct gctacatggt accggtaaaa tcaaataaat
      121 ccgacaacct gagtattgac acctgctggt tcgagtcgcc ggtccacctg gcgggtcaaa
      181 aagtgaaact ctttgtctcg gtgaaaaata atgcggatat catggccgaa aaagtgccgc
      241 tcaaactcac aataaacaat acacaaaaag cggtcagcag ctttgatatt gctccgggca
      301 gctccgctgg caccatcatt acttatgtgg ataaagaacc aggaatacat catggtgtga
      361 ttgagatatc cgatagcccg gttacctttg atgataaatt ttatttttca tacgatatac
      421 agacatcggt acccgtttta tgtatcaacg gccagcaatc gaatgttttt cttaactcgc
      481 tgcttgcaga agactcagct tttagctttc ataacgtatc tgtaaatcag gtagattata
      541 actccttggc ttcctataac ttgataatta tcaatgaaat tccggcagtt tcttccggat
      601 tgtcagaaga gcttcacaaa tttgtgaata gtggcggaca tttgttggtt gtgcctggtg
      661 aaaatatgga aattggcaat taccaaagct ttcttacagt agtaaactca aattattata
      721 ccacactgaa taagcttcca caaaaaattg cagaaataaa tctgcagcat cctatttatt
      781 ccgatgtctt cgaaagtgtt cctcaaaata tcgatcttcc tcaggtttca tcatattttt
      841 cgatgagccg cctcagcaga tcggcacagg attacctgat gaaacttcag aatggggaac
      901 cgtttttaag tgttcaggat tgcggcagcg gaaaagtgtt tcttttgtca gttcccctaa
      961 gggcagaata cagtaatttc cccaaacacg ctttgtttgt gccgaccata tataaaattg
     1021 ccatgatgag caggatgcag tcccggcttt attatatcat tggccgcgac cagcaggtgg
     1081 aagttccgac catgctgaat gacgaaaaag atatggtact caaattaaaa aatactgcag
     1141 gcaatgatga ggtgattccc caattacaaa attataatgg acacatcacg ctggtcttaa
     1201 acgatcaggt aagaaaccac ggaaactata tcctttccaa taacgggcct gttacgggtc
     1261 ttgcattcaa ttacagcaaa gacgaatccg atatgaagtg ttattccccg gaagagatta
     1321 ctgcctttat cgacaagaat aatttgaaaa ataccaagtt actggaaggg aaagcaaaat
     1381 ctctttcggt agtagtgagt gaactgaatc aggggataaa attgtggaaa acttttgtgc
     1441 tgctggcttt actgtttctt gctttagaag ttgtactttt gcgtttatgg aaatgatatg
     1501 cagtaattta tggaagacga agtaacagga aaaaacgaaa atcaggatac cccacaggag
     1561 gctggtgata cccaacaggt attccctaaa acccagcacc ttacggg
//
LOCUS       DAOPZK010000253         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_119306_length_1603_cov_25.371447, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000253 DAOPZK010000000
VERSION     DAOPZK010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119306_length_1603_cov_25.371447"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKH68_09455"
     CDS             complement(<1..467)
                     /locus_tag="PKH68_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HNX13200.1"
                     /translation="MEIIKSNEVIEWAKQFLDKAEKEISPQELKEQNKYAILVQNPND
                     KVLLSKMLDESSQIHDSKKLARRMKILLKRYGVPEFFNSSDHFMLSLFTSFGYWFDFI
                     AVPIFKKRLRSETAAVIINERPDLLTEHLSERRSQKIGQNVNLLGEVVLGDGEA"
     gene            complement(708..>1603)
                     /locus_tag="PKH68_09460"
     CDS             complement(708..>1603)
                     /locus_tag="PKH68_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNX13201.1"
                     /translation="GFPVQKSQLHLMGVPFKSFDILNYRGDKLKAKLEGFLKEENICS
                     ICYSSPNNPSWFCFTEEELQTIGELATEHDVLVIEDLAYFGMDFRENYGVPGESPYQP
                     TVAHYTDNYILILSASKIFSYAGQRIGMLIFSEKTFTRKYPNLKNYFTSDTLGHFIPY
                     GVLYATTAGTAHSAQYALAAMLKTANDGQVDFIKNLHLYGQKARLMKEVFLKHGFYIV
                     YNDDKRELADGFYFTIAYPGLTSGELMTKFLYFGISAISLAITGSENPNGLRACVSFV
                     RLDQIPELDLRLARFVNEMKN"
BASE COUNT          436 a          322 c          345 g          500 t
ORIGIN      
        1 gcttcaccat cgcccagtac tacttcgccc agcaggttta cattctgacc gattttctga
       61 ctgcggcgtt cactcagatg ctctgtgagc aggtcgggac gttcgttgat tatgacggca
      121 gctgtttcgg aacgtaatct tttcttaaat atcggtacgg caataaaatc gaaccagtaa
      181 ccaaacgaag taaataaact gagcataaaa tgatccgacg aattgaagaa ttcgggaact
      241 ccgtaccgtt ttagcaggat tttcatcctt cgggccagtt tcttactgtc gtgaatttgt
      301 gaactctcat ccagcatttt ggataaaagt accttgtcgt tggggttttg aaccagaatg
      361 gcgtatttgt tctgttcttt taattcctgc gggctgattt ctttttcggc tttgtccaga
      421 aactgttttg cccactcgat gacttcgttc gatttgatga tttccatagg tcttgttttg
      481 tcctgccgga gcaggttttt ttagatttat tagtgtgttt ttgttaatta aaaatgaatt
      541 cgtaaaaata caaaaaaatg attattgcaa tcgtgaaaaa ggatatttgt tgcataaata
      601 ttttatgtag atctgatttt cagtttaaga ttacgacggg agtttttatc cacaaaaaag
      661 gtacatcaga taagtcgatg taccattgta atttatcggt gaataaatca gtttttcatc
      721 tcgtttacaa accgggcaag ccttaggtca agttccggta tctgatcgag tcttacaaac
      781 gacacgcagg ctctcagtcc attgggattt tcgctgccgg taatagccag cgatatggca
      841 ctgataccaa aatacagaaa tttggtcatc agttcgccgc ttgtcaggcc cggataagcg
      901 atggtgaaat aaaacccgtc ggcaagttca cgtttatcgt cgttgtaaac gatgtaaaaa
      961 ccatgtttca aaaatacctc tttcatcaat cgtgcttttt gaccgtagag atgcagattt
     1021 ttgataaaat ccacctgtcc gtcgttggct gttttaagca tggcagcaag tgcatattgt
     1081 gccgagtgcg ccgttccggc tgtggtggcg taaagtacgc cgtacgggat aaaatgaccg
     1141 agtgtatccg aagtgaaata atttttcaga tttggatact ttcgagtgaa ggttttttca
     1201 ctgaaaatca gcatcccgat gcgttgtccg gcataactga atattttgga agccgaaagt
     1261 atcagaatat aattgtcggt ataatgtgca accgttggtt ggtagggact ttcgccgggt
     1321 actccgtaat tctcacgaaa atccattcca aagtaagcca gatcttcaat cactaacacg
     1381 tcatgttcgg tagccagttc ccctatcgtt tgcagctcct cttccgtaaa acaaaaccat
     1441 gaaggattgt tgggcgaaga atagcagatg gaacaaatat tttcttcttt aagaaatccc
     1501 tctaatttgg ctttcagctt atcgccgcga taattcagta tatcaaagga tttgaacgga
     1561 acacccatca gatgcagctg tgatttttgc accggaaaac cgg
//
LOCUS       DAOPZK010000254         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_120929_length_1589_cov_25.624511, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000254 DAOPZK010000000
VERSION     DAOPZK010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120929_length_1589_cov_25.624511"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKH68_09465"
     CDS             <1..746
                     /locus_tag="PKH68_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNX13202.1"
                     /translation="SSGDKKSDPKRFTIVGLWGNFILKPPTTRYPEIPEVEDLTMHLA
                     KIARINVVPHSLIRLKSGQLAYITKRIDRIGKSKLHMEDMCQLTERLTEHKYQASYEQ
                     IGKAILRYSVNPGLDVINFCELVLFSFLTGNADMHLKNFSILHDPAIGPVLSPAYDLL
                     STALVNPADDEDLALTLNGKKKRIKRIDFETAFTTLHLDTKQQANLFLKMGKAKKKWL
                     EFIEISFLSDDFKTGFLQLINERFERISV"
BASE COUNT          530 a          304 c          288 g          467 t
ORIGIN      
        1 ttagttcggg tgataaaaaa tcagatccaa agcgttttac aattgttggt ttatggggaa
       61 atttcattct taaacccccg acaacacgct atcctgaaat accggaagtt gaagatttga
      121 ccatgcattt agccaaaata gccagaataa atgttgtacc acatagtttg atccgattaa
      181 aatcgggaca attagcctat attaccaaac gaatcgaccg cattggaaaa tcgaaactac
      241 acatggaaga tatgtgccag ctgactgaac ggctgaccga acataaatat caggcttcgt
      301 acgaacaaat tggaaaagca attcttagat attcagtcaa tccggggctc gatgtgatca
      361 acttctgtga gctggtttta ttctcgtttc tgaccggtaa tgccgatatg cacctgaaaa
      421 acttttcaat cctgcacgac ccggcaatcg gtccggttct ttcgcccgct tatgacttac
      481 tttcaacggc actcgtaaat cctgccgatg atgaagacct ggctctcaca cttaacggga
      541 aaaagaaacg aattaaacgc attgattttg aaacagcctt cactacctta catttagata
      601 cgaagcaaca ggccaatctt ttcctgaaaa tgggaaaagc aaaaaagaaa tggttggaat
      661 ttattgagat aagcttcctc agtgatgatt tcaaaaccgg atttttacaa ctaattaatg
      721 aacgattcga gagaatttca gtgtaattgt tattttacaa atactaattt aaaaaccgta
      781 atcggttact tttacgtcgc agataatcag atagatatac atgtttttgc gaataatcta
      841 tttgttacat atttgtaatg cacatgcatc aagcagatta tcagaaccaa attccatcga
      901 agaaataaag taaaagtcaa aaaaacctca cgggaattat ctataacacc ccaatctgaa
      961 tttttacaat caggttggga tgaattgttt taaacagcta aagtatttat ctacttgttc
     1021 tttcggatat attgacgtaa aaaataatga taacctgttc taatttgagg acctgactgc
     1081 ctcagacata ttttataatc cggattaaac cggatgctct gaaatcgggt ttttcaaatt
     1141 actttccgaa atgatcgctc gggtgagcag taattcttat ccgttttaat tgatcacgga
     1201 caggaatgtc tgtacaccct aatactgagc tgcttgtgct tatctactta actttcctgt
     1261 cttcccttgt caaactttgg gcatacaaaa tctgctttac cgagttgttt caatccccca
     1321 aaaagtcatt tcaacgccaa aaatttacat ttcagtaagt gtttagctct gaaaatatgt
     1381 tgtatagcat aattttgcac cgtaaaacaa actagaatga atatgaaagg aaagattatt
     1441 tcattaacaa tgatgctgct gggaattcaa tcggggtttg cacaggaaag caaaactacc
     1501 gaattgtcgt tgcagcaatg tgtgcagatg gccgttgaaa aaaacattaa tgtgaaaaca
     1561 gcgcgcattg acaaggaaaa gagccaata
//
LOCUS       DAOPZK010000255         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_123543_length_1568_cov_25.514210, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000255 DAOPZK010000000
VERSION     DAOPZK010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123543_length_1568_cov_25.514210"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..483
                     /locus_tag="PKH68_09470"
     CDS             55..483
                     /locus_tag="PKH68_09470"
                     /inference="COORDINATES: protein motif:HMM:NF025629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4359 domain-containing protein"
                     /protein_id="HNX13203.1"
                     /translation="MKKAPYFLLLVLVLLVVVAYLTNPDERQHQAAAKEKLHKVAENL
                     LAKYGIEPGSITGSAINFTEPFVDSLIEKHVSAHNYFLFSTTNIEWEGQSQIIGVGAF
                     KNVFISSKADEMLQQELENYVKEKIRGFKIPGLEGLELGF"
     gene            complement(498..1559)
                     /gene="proB"
                     /locus_tag="PKH68_09475"
     CDS             complement(498..1559)
                     /gene="proB"
                     /locus_tag="PKH68_09475"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNX13204.1"
                     /translation="MAVKIGSNVLTRPDGTLDITRMSALVDQISVLHKLGVQLVLISS
                     GAVASGRSVMGLSKKMDIVEQRQLYSTVGQAKLINHYWELFREHGIIVGQVLTTKTNF
                     EGRSQYLNQRNCIRIMLENKVIPIVNENDAVSVSELMFTDNDELSGMIASMLGMEALV
                     ILSNIDGIFDGAPSNPDSKVIRNIRKGQDLSDFIQTSKSTFGRGGMQTKTSIARKIAD
                     EGIEVIIANGKKENILVELLDKRKDTVCTHFEASGSEVSSVKKWIAHSDGFAKGEIHI
                     NENAEKALTGPKATSLLPVGITGITGNFEKDDIVKIIGEKGQTIGVGKAQYSSEKAVP
                     LIGRKNQKPLVHYDYLYLE"
BASE COUNT          406 a          351 c          331 g          480 t
ORIGIN      
        1 tgcggcaggc aggctttagg gggttagggg gcagatttaa aaaattatag acagatgaaa
       61 aaagcacctt acttcctttt attagtactt gttttgctgg ttgttgtagc ttatctgacc
      121 aatccggacg aacgtcagca tcaggcggct gctaaagaaa aactgcataa agtggctgaa
      181 aatttactgg ccaaatacgg tattgaaccc gggtctatca ccgggtcagc aattaatttt
      241 accgaaccat ttgtcgatag tctgatcgaa aagcatgttt cggctcacaa ttatttcctt
      301 ttttcaacta ccaacataga gtgggaggga caaagtcaga ttattggcgt gggtgcgttc
      361 aaaaatgttt ttatttcctc caaagccgac gaaatgctgc aacaggaact cgaaaattat
      421 gtgaaggaaa aaatcagggg gtttaaaatt ccgggtttag agggattgga attgggattt
      481 taaaaaacat caacaactca ctccagatat aaataatcgt aatgtacaag cggtttctgg
      541 tttttcctgc cgattaacgg gaccgctttt tcgctgctgt attgtgcctt tcctactcct
      601 attgtctgtc ctttctcacc aatgattttg acaatgtcat ctttttcaaa attgccggta
      661 atccctgtga tgcccaccgg caggaggctc gtggctttcg ggccggtgag tgctttttcg
      721 gcattttcat tgatatgaat ttcacctttg gcaaatccat cgctgtgagc aatccatttt
      781 ttcacactcg aaacttccga acctgatgct tcgaaatgag tgcaaaccgt atcctttcgc
      841 ttatcgagca attctaccag aatattctcc tttttaccgt tggcaatgat aacctcaatg
      901 ccttcgtcag ctattttgcg tgctatgctt gttttggttt gcatccctcc acgtccgaaa
      961 gtggattttg aggtttgaat aaaatcagat aaatcctgcc ctttccttat atttctgatt
     1021 actttcgaat ccggattgga gggagcccca tcaaatatgc catcgatatt actcaaaatc
     1081 accaatgctt ccatgccgag catggaggca atcatccccg atagttcgtc gttgtctgta
     1141 aacatcagtt ccgaaaccga aacggcgtca ttttcattaa cgataggtat caccttgttt
     1201 tcaagcatga tacggataca gttacgttgg ttcagatact gcgagcgccc ttcaaagttg
     1261 gttttggtag tcagcacctg ccctacaatg atgccgtgct cgcgaaaaag ttcccagtaa
     1321 tggttgatca gcttagcctg gcctacggtg gagtaaagtt gtcgttgttc cacaatatcc
     1381 attttttttg ataatcccat cacacttcgt cccgaagcta ccgctcccga cgaaatcagc
     1441 accaactgca caccgagttt atgcagtacc gatatctggt caactaatgc cgacatacgg
     1501 gtgatgtcga gtgtgccgtc agggcgtgtc aatacgttac tgccaatttt tacggctatt
     1561 tttttata
//
LOCUS       DAOPZK010000256         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_123811_length_1566_cov_23.785572, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000256 DAOPZK010000000
VERSION     DAOPZK010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123811_length_1566_cov_23.785572"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..632
                     /locus_tag="PKH68_09480"
     CDS             273..632
                     /locus_tag="PKH68_09480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNX13205.1"
                     /translation="MKKFLKSFVFAAKGIKSVISSETNMKVHTGISVFVIIMGLVFHI
                     STVEWLACILCIGLVFTVEIFNTSIETLVDLVSPQKHHLAGKTKDIAAGAVLVAAIMS
                     VAVGIIIFLPKVIHLIF"
     gene            647..1336
                     /locus_tag="PKH68_09485"
     CDS             647..1336
                     /locus_tag="PKH68_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNX13206.1"
                     /translation="MNEWLKSFFELFYPNLCLICGESLVTGEIQLCLKCQSQIPKTNY
                     HLLPENPVEKRFWGKVPVANASSYFFFQKGSDFQKLIHELKYRGNREIGTIIGKYAAV
                     DLLENPEYHDVDWIVPVPLHPKKLAKRGYNQSEMICTGISVILEKPVNTSNLVRVQEN
                     TTQTKKSVYERYENTQGIFHLTNPEEFRDKHILLVDDVLTTGSTLEACIQTLLLAENC
                     KVSVFTLAVAI"
BASE COUNT          475 a          310 c          309 g          472 t
ORIGIN      
        1 ccgtcaatga ctttatagac cttacggtat tcccgggagg agttagccac cgaagtcgat
       61 atggcggaag gagacggttt ttccttgcaa ttgaccacca caaacatcat taatataaga
      121 aggaaatggg caaactttcg gaaattcata tacattttca ggattaagat tattaagcag
      181 caaaaatacc attttgaatt tggatttccg tcacaaaggg tatcaaacaa attatttttt
      241 atacttttgt caattatcaa ttattttcgc tgatgaaaaa atttttaaaa agttttgtat
      301 ttgctgccaa aggaataaaa tcagttattt cttcagaaac aaacatgaaa gtacacacag
      361 gtatttctgt ttttgtaata attatgggtt tggtatttca catatccacc gtagagtggc
      421 tggcctgtat tttatgcatc ggtctggttt ttacagttga gatattcaac acgtctattg
      481 aaacgctggt tgatttagta tcccctcaaa agcaccatct ggccgggaaa accaaagata
      541 tagcggcagg agctgtattg gttgcagcta tcatgtcggt tgctgtaggt atcatcatat
      601 ttcttccaaa agtaattcat ctcatttttt aattcctgat tggaaaatga acgaatggct
      661 gaagtctttt ttcgaacttt tttatccaaa tctgtgcctg atatgtggtg aaagtttggt
      721 gacgggtgaa atacaacttt gcctgaaatg tcaaagtcaa attcccaaaa caaattatca
      781 tctcctgcct gaaaatccgg tagaaaaacg tttttggggg aaagtaccgg tggctaatgc
      841 cagctcctat ttcttctttc agaaaggttc cgactttcag aaacttatac atgaactgaa
      901 atatcgcggt aaccgggaaa ttggaacgat tatcggtaaa tatgctgccg ttgacttgct
      961 ggaaaatccg gaatatcacg atgtcgattg gattgtacct gtacctctgc atcccaaaaa
     1021 actggcaaaa cggggctata accaaagtga aatgatttgt accggtattt cggtcattct
     1081 tgaaaaaccg gttaatacat ctaatcttgt acgcgttcag gaaaatacca ctcaaaccaa
     1141 aaaatcggtt tatgaacgct acgaaaacac gcagggtatt ttccacctga caaatccgga
     1201 agaatttcgg gataaacaca ttctacttgt agatgatgtg ctcaccaccg gctccactct
     1261 tgaagcctgc attcaaactt tgcttcttgc cgaaaattgt aaagtgagtg tgtttacact
     1321 ggctgttgca atttagcaat gcaaaaagag caaccacatt gctgtaattg ttcttcatta
     1381 aaacggcggc tatcggattt tatccctgat tgttcggggc tcccaccagt aacgcattac
     1441 cctgtctgcc ggcaggcaga cagcgtcgtg attggtctta ttccgatcta tcggaaccgg
     1501 aatggaaaaa accggttcgg attatcctcc cggttctgaa actccatcct tatacaaaca
     1561 aaaaaa
//
LOCUS       DAOPZK010000257         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_123935_length_1565_cov_23.272848, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000257 DAOPZK010000000
VERSION     DAOPZK010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123935_length_1565_cov_23.272848"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..885)
                     /locus_tag="PKH68_09490"
     CDS             complement(61..885)
                     /locus_tag="PKH68_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNX13207.1"
                     /translation="MKSAAKKAFPTQKQNFPTVADTTNIYNDMLNDESDDLMENHPAG
                     DIFNDNWSSERINPYKVPIQSMPDSVLIDCSDFVMPVTGGRVSSKFGPRRYRYHYGID
                     VAIQPGNEIRSAFNGKVRVIDYDAGGYGNYVVVRHDNGLETVYAHLSFVKAKLNQHVK
                     AGEELGLSGNTGRSTGPHLHFETRYVGNAFNPATLIDFSEGKVFASNYTLTKRKNFYY
                     QQAARTQSLAKYYKIRKGDNLGRIAARNGTTVTKLKRLNGITKGNKLQPGQKLKIR"
     gene            complement(993..1454)
                     /gene="ndk"
                     /locus_tag="PKH68_09495"
     CDS             complement(993..1454)
                     /gene="ndk"
                     /locus_tag="PKH68_09495"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNX13208.1"
                     /translation="MEKTLVIIKPSALQRALTGEVVKRFEQKGLRLAGIKMMQLDDKI
                     LDEHYAHLADRPFFQRIKDSMMITPVIVTCWEGKDAVKVVRAMTGSTNGRDAVPGTIR
                     GDFSLSVQENIVHTSDSVETAVVELARFFSDDEIFTYNYQSLPFLYANDEI"
BASE COUNT          413 a          319 c          353 g          480 t
ORIGIN      
        1 ataaaaaaga ggatactctt ttcgaatatc ctcttctgta atataataat aagtaagtac
       61 ttatctgatt ttcagtttct ggccgggttg cagtttattg ccttttgtga tgccgttaag
      121 tcgtttcagt ttggttactg tagtaccgtt gcgggctgca attctaccca ggttgtcgcc
      181 tttgcgaatt ttatagtact tggccaacga ttgtgttctg gctgcctgct gatagtagaa
      241 gtttttccgc ttggtgagtg tataatttga tgcaaacact tttccttcgg aaaaatcaat
      301 cagcgtagcc ggattgaagg cattgcccac gtaacgggtt tcgaagtgga ggtgaggtcc
      361 tgtggatcgg ccggtattgc cgctgagtcc gagttcttca ccggctttta catgctggtt
      421 caattttgct tttacaaaac tgaggtgagc ataaaccgtt tccagtccat tgtcgtgacg
      481 tacaaccacg tagttgccat atccgcctgc atcgtaatcg ataacacgaa ccttgccgtt
      541 gaatgccgag cgtatttcgt tgcctggctg gatggccaca tcgataccgt aatgataccg
      601 gtaacggcgc ggaccgaatt tggaggaaac tctaccaccg gtaacgggca tcacaaagtc
      661 ggaacagtcg ataagaaccg agtccggcat gctttgaata ggcactttat agggattgat
      721 gcgctcgctt gaccagttgt cgttgaatat gtcaccggcc gggtggtttt ccatcaagtc
      781 atccgattcg tcattcaaca tatcgttgta tatgttggtg gtatcggcta cggtggggaa
      841 attttgtttt tgagtgggga aagctttttt tgcagccgat ttcatattgt cgggtgctgc
      901 atttgcggaa accgcaaaaa gctgtgcgtt tgtttggatt gagatcaggc ttactattga
      961 tgtaagaatt aaatacttaa aatgcattgg cgttaaattt cgtcgttagc gtataaaaat
     1021 ggcagtgact ggtagttgta agtgaaaatt tcatcatcgc taaaaaaacg tgcgagctca
     1081 acaacagctg tttcaaccga gtcggacgta tgaacaatat tctcctgcac acttaaacta
     1141 aaatcaccgc gtatagttcc gggtactgca tcacgaccat tggttgagcc ggtcatggca
     1201 cgtacaactt ttacagcatc cttcccttcc cagcaagtga ctatcaccgg agtaatcatc
     1261 atcgagtctt taatgcgttg gaagaaagga cggtcggcca aatgagcata atgctcatct
     1321 aaaattttat catcaagctg catcatttta atacctgcca gtcgaagccc tttttgttcg
     1381 aagcgtttaa ccacttctcc cgttaaggcg cgctgcaatg cagaaggctt gatgattacg
     1441 agggtttttt ccattaattt ttatgttgaa cttttaattc aaaaataaaa tattctcaaa
     1501 gataaaataa atcaggcaaa caaacaaagc gaattgacat taaaaatatt tttttaactt
     1561 attgc
//
LOCUS       DAOPZK010000258         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_125171_length_1555_cov_21.988000, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000258 DAOPZK010000000
VERSION     DAOPZK010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125171_length_1555_cov_21.988000"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKH68_09500"
     CDS             <1..306
                     /locus_tag="PKH68_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNX13209.1"
                     /translation="PFMSDRYLSIENAKYIFGNMKSIGEEVEFKKDGIIQMRAKEVLG
                     KTIGLLEQIQKEGLFTALEKGIFAGIKRSREGGKGLNGVFIKSDNYYNPFIDSMLKR"
     gene            322..1113
                     /locus_tag="PKH68_09505"
     CDS             322..1113
                     /locus_tag="PKH68_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HNX13210.1"
                     /translation="MSGGLYSTEKNDFDQTLDLTKIKPYGDTMNDGKTQISFTLPVPA
                     GDEAIEAAKQLLKQMGFDNPQVVFHKELTPGFTFFNCYGNYTGTIDYTGIYVPKVESV
                     SWNMHETDEFIREKISRKIVVLGASTGTDAHTVGIDAIMNMKGFAGHYGLERYEMFET
                     FNMGSQVANEDFIAKAIELKADALLVSQTVTQKDIHIKNLVELVELLEAEGLRDKIIL
                     VCGGPRISHELAKELGYDAGFGMNSYAGDVASYLAQELHRRINRE"
     gene            1140..>1555
                     /locus_tag="PKH68_09510"
     CDS             1140..>1555
                     /locus_tag="PKH68_09510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNX13211.1"
                     /translation="MDKDQIREVIAKRVAKELKDGDVVNLGIGLPTQVVNYLSPDVHV
                     ILQSENGLIGMGRTPEPGEENPYYVNAGAGAVLPNAGAACFDSATSFAIIRGGHVDVS
                     VLGALQVDENGDLANWMIPGKIVPGMGGAMDLLIGTR"
BASE COUNT          506 a          286 c          388 g          375 t
ORIGIN      
        1 ccctttatgt cagaccgtta cctttccatc gaaaatgcaa aatacatttt tgggaatatg
       61 aaatcaatcg gtgaggaagt ggaattcaaa aaggacggca ttattcaaat gcgggcaaaa
      121 gaagtgcttg gcaaaaccat tggactgctc gagcaaattc agaaggaagg cttatttacc
      181 gctctggaaa agggaatatt tgccggtatc aagcgttcaa gagaaggcgg gaaaggtttg
      241 aatggagttt tcattaaaag tgacaattac tacaaccctt ttattgacag catgttgaaa
      301 cgataaaaat aaacaaaaaa tatgagtgga ggattatatt caaccgaaaa aaatgatttt
      361 gaccagacac tggatttgac gaagattaaa ccctatggtg acaccatgaa tgacggaaaa
      421 acccagatca gtttcacatt gccggttccc gccggtgacg aagcgataga agcagctaag
      481 caattgttga aacaaatggg ttttgacaat ccgcaggtgg tgtttcacaa agaacttacc
      541 ccgggattta ctttttttaa ttgctacgga aactacacag gtacaattga ttatacggga
      601 atatatgtac caaaagttga atcggtgagc tggaatatgc acgaaaccga tgaatttatc
      661 cgcgaaaaaa tcagccggaa aattgtggta ttgggagcca gcacaggaac tgatgcacat
      721 actgttggga tagatgcaat tatgaatatg aaaggatttg ccggtcatta cgggctggaa
      781 cgttacgaga tgtttgaaac tttcaatatg ggaagtcagg tcgcaaatga agattttata
      841 gcaaaagcca ttgaactaaa agccgatgcg ctgctggttt cacaaaccgt aacgcaaaaa
      901 gatattcata tcaaaaatct ggtggaactg gtggaattgc ttgaagccga aggacttcgc
      961 gataagatca ttttggtatg cggagggccc cgtatctctc acgaactggc aaaagaactg
     1021 ggatacgatg caggatttgg catgaattcc tatgccggtg atgtggcttc gtacctggct
     1081 caggaactgc accgaagaat taaccgggaa taaaaacaca ttcataaatc tcatcggata
     1141 tggacaaaga ccaaataaga gaagtaatag caaaacgagt ggcaaaagaa cttaaagacg
     1201 gtgatgttgt caatttaggt attggactgc caacacaggt ggtcaattac ctttcacccg
     1261 acgttcatgt tatactacag tccgaaaacg gtttgattgg catgggaaga acacctgaac
     1321 cgggcgagga aaatccttac tatgtcaatg caggtgccgg agcagttctg ccaaatgcag
     1381 gagcagcatg tttcgacagt gcaacctcgt ttgccatcat tcgcggtggt catgtagatg
     1441 tgagcgtgct gggggcatta caggtggacg aaaatggtga cctggcaaac tggatgatac
     1501 cgggaaaaat agttccgggt atgggtggag ccatggacct gctcatcggg acacg
//
LOCUS       DAOPZK010000259         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_125910_length_1549_cov_21.809237, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000259 DAOPZK010000000
VERSION     DAOPZK010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125910_length_1549_cov_21.809237"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1470
                     /locus_tag="PKH68_09515"
     CDS             115..1470
                     /locus_tag="PKH68_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNX13212.1"
                     /translation="MKGKIILFTMMVLGIQAGFAQESKTTELSLQQCVRMAVENNINV
                     KTSRIDKEKSQYKKEESRAALLPKINFNGGFTDNIALPTTVLPGEFLGQPGKTIAVQM
                     GSQYNANAALSISQILYNQTALTALKLTKKSEELSSLSVEKASEEIAFEVARLYALGQ
                     TTSEQLNLINQNIKRTERLRDITKIIVQNGVGKQVDLDRVNVNLENLYTQLNNTQTAR
                     EQQLYMIKYMLDLPLEENVVLTDTATMPLLTSEPILQSDFSNHVNIRLLESQKELNIL
                     NKKLINNGYLPTVSFSGQMAYQGLRKDFSTYFKSGEVNKWYPMSNIAINVSVPVFDGF
                     EKRSKARQATLDLQKTQATLESAKESISMNYKNALNNYINNREIVRRQKQNLELAEKV
                     FKESSLKYREGLAGMSSLLQDEMSLSSAQGGYLTALYNFKEAELKIMSINGDIRKLFE
                     K"
BASE COUNT          511 a          315 c          344 g          379 t
ORIGIN      
        1 ttcaatcccc caaaaagtca tttcaactcc aaaaatttac atttcagtag gctttgcatt
       61 taaaatatat gttttacagc ataattttgt gtcgttaaac aatatagtat gaatatgaaa
      121 ggaaagatta ttttatttac aatgatggta ctgggaattc aggcgggctt tgcgcaggaa
      181 agcaaaacta ctgaattgtc gttgcagcaa tgcgtgcgga tggctgttga aaacaacatt
      241 aatgtgaaaa catcgcgcat tgataaggaa aagagccaat acaagaaaga agagtcgcgg
      301 gcggcgttgc ttccgaaaat aaatttcaac ggaggcttta ccgacaatat cgcgttgccg
      361 acaactgtac ttccgggtga gtttctgggg caaccgggta aaacgattgc cgtacaaatg
      421 ggaagtcagt acaacgcgaa cgccgcgctg agcatcagtc agattttgta taaccagaca
      481 gcactcacag ctctcaaact gaccaaaaag tcggaagaac tgagcagcct gagcgttgaa
      541 aaagccagcg aggaaatcgc gtttgaagtt gccagacttt atgcactcgg ccagacaacc
      601 tccgaacagc ttaatctgat aaaccagaat atcaaacgta ccgaacgcct gagggatatt
      661 actaaaataa tcgtacagaa tggagtggga aaacaggtgg acctggaccg tgtgaatgta
      721 aatctggaaa atttgtacac tcagctgaat aatacgcaga cagcccgtga acaacaactt
      781 tatatgatta aatatatgct tgacttaccg cttgaggaga atgtggtgct gaccgatacg
      841 gctacaatgc cccttttaac cagtgagcca atacttcagt ctgatttttc gaatcatgtg
      901 aatattcgtc tgctcgaatc acagaaggaa ctcaatattc tgaataagaa gcttattaac
      961 aatggatatc tgcctacagt ttcattctcg ggtcagatgg cttatcaggg tttgcgtaaa
     1021 gatttcagca cctattttaa aagtggagaa gtcaataagt ggtatcccat gtccaacata
     1081 gccatcaatg tttcggtgcc ggtattcgat ggttttgaaa agcgctcgaa agcgcgtcag
     1141 gctaccctcg atctgcaaaa aacgcaggct acgctcgaaa gcgccaaaga aagcatcagc
     1201 atgaattaca aaaacgcgct gaataattac ataaacaacc gcgaaattgt tcgccgccag
     1261 aaacaaaacc tcgaactggc cgaaaaagtg tttaaggaat catccctgaa ataccgcgaa
     1321 gggttggcgg gtatgagcag cctgctgcag gatgaaatga gtctgagcag cgcgcagggt
     1381 ggatatctga cagcgctcta caacttcaag gaagccgaac taaaaattat gtcgatcaac
     1441 ggagatatca gaaaattgtt tgaaaaataa atatgtttca agttacgagt tactagttac
     1501 aagtttcacg ttacaagttt caagttacgg gttactaatc accaatcac
//
LOCUS       DAOPZK010000260         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_128726_length_1527_cov_19.300272, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000260 DAOPZK010000000
VERSION     DAOPZK010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128726_length_1527_cov_19.300272"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..509
                     /locus_tag="PKH68_09520"
     CDS             168..509
                     /locus_tag="PKH68_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNX13213.1"
                     /translation="MNLNQLIKLVNGSILYDSLPGLELSYAFASDLMSDALRWYKENM
                     ILITGLATIQTIRTAELAGISCIVIARGKKVTDEMMELAVESHISIVTSPMSVFEISG
                     KLYQNGIKPLY"
     gene            509..919
                     /locus_tag="PKH68_09525"
     CDS             509..919
                     /locus_tag="PKH68_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNX13214.1"
                     /translation="MQMQFDIQGGDFSAAGEASSEVKKALKQLNIQPQTLRRIAVALY
                     EAEVNVVAHAYRGTMTVDISPARIRVQLQDEGPGIADIGKAMEEGFSTASDKVREMGF
                     GAGMGLPNIKKNSDEMHLTSTPGTGTTLEIIVNF"
     gene            936..>1527
                     /locus_tag="PKH68_09530"
     CDS             936..>1527
                     /locus_tag="PKH68_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNX13215.1"
                     /translation="MDKPHYVHAIQINTDKCIGCSHCMRVCSTQAIRIVGGHARIMPE
                     RCVDCGECYSVCPVAAVYVKSDSLETHLQQYVYKIALVPSVFFGQFPTKYSVSQISVA
                     IRQLGFNEVFEVEQAVDFVKEEYKLKSDEPDSPKPVISSFCPSVVRLIQVMFPSLTEN
                     VFQLKAPHDIAAIYLRDINSTTNIPPEDIGIFYISPC"
BASE COUNT          438 a          325 c          357 g          407 t
ORIGIN      
        1 actgaatttc aaagaaagca gaacgtaaag ttttgctttt ttatttgctg aaaatcaacc
       61 caaaaggagt gattttattt tgattttatt atggaaaaat ttcattaata agaaaagtat
      121 tttctatatt tgtgcctaat atgatgtttc taaagaaatt attccctgtg aatttaaacc
      181 aattaataaa attagtaaac ggatcaattt tgtatgacag tcttcctggg ctggaattga
      241 gctatgcttt tgcctcggat ctgatgagcg atgcacttcg ctggtacaaa gagaatatga
      301 tccttattac agggctggcc accattcaaa ctatccggac tgccgaactg gcaggaatat
      361 cctgcatagt gatagcacgc ggcaaaaagg tgaccgacga aatgatggag ctggctgtag
      421 agagccatat cagcatagta acttcgccca tgtcagtctt cgaaatctca ggtaaattat
      481 atcaaaacgg aataaaaccg ctttactgat gcaaatgcag ttcgacatac aagggggaga
      541 tttcagcgcg gcaggcgagg ccagttcgga agtgaaaaaa gcgctgaagc agctgaatat
      601 tcagccgcag accttgcgcc ggattgccgt ggcactctat gaagccgaag tgaacgtggt
      661 ggcgcacgcc tatcggggta cgatgactgt ggatatttca cccgcaagaa ttcgggtgca
      721 gctgcaggac gaaggacccg gtattgccga tataggcaaa gccatggaag aaggattttc
      781 caccgcctcc gacaaagtcc gcgaaatggg cttcggagct ggaatgggac tgccgaatat
      841 taagaaaaat tcggatgaaa tgcacctgac ttccactccc ggcacgggaa ctacactcga
      901 gataatagtt aacttttaaa tagattttcc gaaaaatgga taaaccgcac tacgtgcatg
      961 caatacaaat taatacagat aaatgtattg gttgttcaca ctgcatgcgt gtctgttcta
     1021 cacaagccat ccgcattgtg ggtggtcacg cccgcatcat gcctgagcgt tgtgtggatt
     1081 gcggagagtg ttacagcgtg tgtcccgtgg cggcggttta tgtcaaatcc gattcgctcg
     1141 aaactcacct gcagcagtat gtgtataaaa tagcgctcgt accgtctgtg tttttcggac
     1201 agttcccaac caaatattcc gtttcacaaa tctcggtggc tatccgtcag cttggcttca
     1261 acgaggtatt tgaagtggag caggccgttg attttgtaaa agaagaatat aaactgaaaa
     1321 gcgatgagcc cgatagtccg aagccggtta tcagttcgtt ctgcccatca gtggtaaggc
     1381 ttattcaggt tatgtttcca tcgcttacgg agaatgtatt tcagctcaaa gcaccgcacg
     1441 atattgccgc catttaccta cgggatatca attcaaccac caatattcct cccgaagata
     1501 tcggcatatt ctacatttcg ccttgtg
//
LOCUS       DAOPZK010000261         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_129132_length_1524_cov_44.548673, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000261 DAOPZK010000000
VERSION     DAOPZK010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129132_length_1524_cov_44.548673"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1176
                     /locus_tag="PKH68_09535"
     CDS             139..1176
                     /locus_tag="PKH68_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNX13216.1"
                     /translation="MSLNKEAYIRYKTIDECLNDKFSLYPTIYDLIEACEKKLGKNFS
                     ISSIQKDIKAMKEDEALGFNAPIKFSRQYNGYYYSDPDFSINSIPLNSSELEALEAVS
                     DVLTAFTGSRISEYYNQAVQKITASIKEKTLHKGKNRKFIQTDAQVRHKGFEYFELLL
                     HAVMEKIPVCFIHYSYNKRCFNSVIAHPVLLKEFQNKWYLVAYSEQHKCLRTFGLDRI
                     SDPLMLKKEFHDTSDEIIDNYFSDIYGVYPLPKAVKQKITFLAGPFIAEYIKANPIHA
                     SQTIQKTGEHGGVFFELELIPSQELINFFVQFGPDLVVLSPELVHNKVLETVKKSLLN
                     CTFHANIESFI"
     gene            1151..>1524
                     /locus_tag="PKH68_09540"
     CDS             1151..>1524
                     /locus_tag="PKH68_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNX13217.1"
                     /translation="MPTSNLLYNLPRYKTYYDPPRLLRIILNEKFTRIDFGYQADSKY
                     IRGGWVTISPKTFIRSKGSDEKLVLTQAVNIPYTPDKHHFKSIVDNLSFSLFFPPLPD
                     GVSSFDLIEEVKETPTSFNYHD"
BASE COUNT          437 a          333 c          276 g          478 t
ORIGIN      
        1 ttggtgactg cggtagaata atcccgggtt ttcatatttc ctgtatcttg ttacctgttt
       61 tgatttgtta gcaaaacaaa attttcaaac gcagtccatt tgcgttttgt tatttctttt
      121 gcttatattt gcattactat gtcattaaat aaagaagcgt acatccgcta caagaccatt
      181 gatgaatgtc ttaacgacaa gttttcactc tatcctacca tttacgacct tattgaggcc
      241 tgtgaaaaaa aacttgggaa gaatttttct atctcttcca ttcagaaaga catcaaggca
      301 atgaaagaag atgaggcgtt gggcttcaat gcccccatta aattcagcag gcagtacaac
      361 ggttactact attccgatcc tgatttttcc ataaatagca ttcctttgaa cagctctgaa
      421 ctcgaagctc tcgaagcggt gtcagatgtg ttgactgctt ttacgggcag tcgtatcagc
      481 gaatattaca accaggcagt acaaaagatt acggcatcca taaaagaaaa aacacttcat
      541 aaaggtaaaa atcgaaaatt catacaaacc gatgcccagg tgcgccacaa aggttttgaa
      601 tatttcgaac tgctccttca tgccgtcatg gaaaaaatac cggtatgttt cattcattat
      661 tcatataata agcgttgttt caattctgtc attgcacatc cggttcttct caaagaattt
      721 cagaacaagt ggtacctggt tgcatactcc gaacagcata aatgtctgcg cacattcggt
      781 ctcgaccgca tcagcgatcc actgatgttg aagaaagagt ttcacgatac gtccgatgaa
      841 ataattgaca actacttttc agacatttat ggtgtgtacc cattgcccaa agctgtaaaa
      901 cagaagatca cctttctggc tggtcctttt attgctgaat acataaaggc aaatcccatc
      961 catgcatcac aaaccattca gaagaccggc gagcatgggg gtgtcttttt tgaactggaa
     1021 cttatccctt cccaggaact tattaatttc tttgttcagt tcggtcccga tttggtggtt
     1081 ctttctcccg aactggttca caacaaagtg ctggagaccg ttaaaaaatc tttgttaaat
     1141 tgtacatttc atgccaacat cgaatctttt atataacctc cccaggtaca aaacgtatta
     1201 tgatcctccc cgtttgctga ggattattct gaacgaaaaa tttacccgta tagattttgg
     1261 ctaccaggcc gattcaaagt atattcgtgg cggttgggtt accatatcgc ctaagacctt
     1321 tattcgtagt aagggctctg atgaaaagct ggtgcttacc caggcggtga atattcctta
     1381 tactcccgat aagcatcatt tcaaaagcat cgttgataat ctttcgttct cattgttttt
     1441 tcctcctctc cctgatggcg tttcatcctt tgatctcatt gaagaagtaa aggaaactcc
     1501 tacttcattc aattatcacg acat
//
LOCUS       DAOPZK010000262         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_130405_length_1515_cov_32.435616, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000262 DAOPZK010000000
VERSION     DAOPZK010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130405_length_1515_cov_32.435616"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1515)
                     /locus_tag="PKH68_09545"
     CDS             complement(<1..>1515)
                     /locus_tag="PKH68_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNX13218.1"
                     /translation="KSTLKITNLSPYIDSKSVQVRINNNNITVLSVNHQLNFLDSLKK
                     SKEADALLQQLEITKDKLQAENTKLEVINDELNFLKENIKIGGSNEGVTLTKLKETAA
                     FYSQQVNSLKTRKAEVDKNISKLNKTLDDIAQQLRQLGESEKEPMGEVVVAIDAKSSA
                     SINIELSYYVNNAGWFPSYDIRSTSIKDPIQLTYKSNIQQSTKEDWKNVRLKVSSLNP
                     NLGNTPPQLKTYFIDYYTAPPRYDLNFNRQIQGRITDVNNEPLIGVTVRVLGSTIGTV
                     ADINGNFSITQPANGNMLEFSYIGMVPQTVPITQNFMNIVMQEDAKNLEEVVVVGYGT
                     QKKSLLSGVSGKLAGVAVTNNKKKEVSAPLPVVQTENQTAVEFEIKTPYTILSDNKSI
                     TAEMDSYELPAQYEYFSIPKVDKDAFLLAYIRDWEKYNLLEGEANIFYENSFVGKTIL
                     DTRAMSDTLNISLGRDKNVQVKREKIKDFSTKKFLGSKKEETRVWKTTVRNNKKQ"
BASE COUNT          373 a          315 c          292 g          535 t
ORIGIN      
        1 ttgttttttg ttatttctta cggtcgtttt ccaaacccgg gtttcttcct ttttgcttcc
       61 caggaatttt ttcgtactga aatccttaat tttctcacgt tttacctgta cgtttttatc
      121 acggccaaga gaaatattta aggtatcgga catagcgcgt gtatctaaaa tggtctttcc
      181 tacaaaactg ttttcataaa agatatttgc ctcgccttcg agcaggttgt atttctccca
      241 atcacgaatg taggcaagca gaaaagcatc tttatccact ttaggaatgc tgaagtattc
      301 atactgagcc ggcagttcat acgaatccat ctcggctgta attgatttgt tgtcagaaag
      361 gatggtgtac ggtgttttta tttcaaactc aacggctgtc tgattttctg tctggacaac
      421 aggcagtgga gcgctcactt ctttcttttt attattcgtc acagccaccc cggctaattt
      481 accactaaca cctgaaagga gtgatttctt ttgtgttccg taaccaacta ccactacttc
      541 ttccagattt tttgcgtctt cctgcatgac aatattcata aaattctgcg tgatgggcac
      601 tgtctgcggc accattccaa tgtatgaaaa ttcaagcata ttaccatttg caggttgtgt
      661 gatgctaaaa ttaccattga tatcggccac tgtgccaatg gtagaaccca ggactctgac
      721 cgttactccg attaacggtt cattattgac atcagttatc cgtccctgaa tttgtctgtt
      781 gaaattcaaa tcgtaacgcg gcggggctgt gtaataatca ataaagtaag ttttaagctg
      841 gggcggcgtg tttcctaaat taggattgag tgaactcact ttcagcctga catttttcca
      901 gtcctcttta gtgctttgct gaatgttcga tttgtacgtg agctgaatgg ggtctttgat
      961 actggtggaa cgaatatcgt atgaagggaa ccaaccggcg ttatttacat aatagctgag
     1021 ttcaatgttg atactggcag aagattttgc atcaatagcc accacaactt cacccatcgg
     1081 ttctttctcc gactcgccca gctgcctgag ctgttgggca atatcgtcca gggttttgtt
     1141 caattttgaa atgtttttgt caacttctgc tttcctggtt ttcagagaat tgacctgctg
     1201 actgtaaaaa gcagctgttt cctttaattt tgtcagggtg actccttcat tacttccacc
     1261 tattttgatg ttttctttca aaaaattcaa ttcatcgttg atgacctcaa gtttggtatt
     1321 ttcagcctgc agtttatcct tcgttatttc caactgttgc aacaaggcat ccgcttcttt
     1381 cgatttcttt aaactatcca gaaaattcag ttgatgattt accgaaagaa cggtaatatt
     1441 attgttattt atccgtacct gtacactttt agaatcgatg taaggagaga gatttgtaat
     1501 tttcagcgtt gattt
//
LOCUS       DAOPZK010000263         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1290
            NODE_130963_length_1511_cov_30.565247, whole genome shotgun
            sequence.
ACCESSION   DAOPZK010000263 DAOPZK010000000
VERSION     DAOPZK010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854495
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:04:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,886
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130963_length_1511_cov_30.565247"
                     /isolate="AMR_MDS_1290"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..749
                     /gene="trpC"
                     /locus_tag="PKH68_09550"
     CDS             3..749
                     /gene="trpC"
                     /locus_tag="PKH68_09550"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNX13219.1"
                     /translation="MEVAERKLLKSISDMEKEPLFSRKCISLKKSLTGSASGIISEFK
                     RKSPSKGWIHPDADVIEITRGYCDAGAAGISVLTDYEFFGGSPADLTAARSVVNCPIL
                     RKDFVIDEYQLYEAKVVGADVILLIAAALTPDKTKNLARQAKDLGLEVLLEVHNREEL
                     DCANEFVDLLGVNNRNLKTFEVDINISKELAEFIPKEFIKISESGISNPQTVIELKSF
                     GYQGFLMGENFMKEQNPADALKRFICPLTP"
     gene            898..>1511
                     /locus_tag="PKH68_09555"
     CDS             898..>1511
                     /locus_tag="PKH68_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNX13220.1"
                     /translation="MQIKICGLRDPENIREIAALQPDFMGFIFYPNSPRYVGQLDAGS
                     LTSLPTSIKKTGVFVNENLENILTAISKYNLDAVQLHGADNKKLCRKIRQEAKTMVIK
                     VFPIMDASNFRVTGDYEEVADYFLFDTKTDLYGGSGQKFNWNILQEYTGNKSFLLSGG
                     IGADDVKAIRAIEHPLLAGVDLNSKFELKPGLKNVALLKQFIDEL"
BASE COUNT          479 a          282 c          338 g          412 t
ORIGIN      
        1 tgctggaagt tgccgaacgg aagctattaa aatccatttc agatatggaa aaagaacctt
       61 tattttcaag gaaatgcata tctttgaaaa agagtttgac tggttcagca tccggcatta
      121 tttcagaatt caaacgcaaa tcaccatcca aaggctggat acatccggat gctgatgtga
      181 ttgaaattac ccggggctat tgtgatgctg gagctgccgg aatttctgtt ctgacagatt
      241 atgagttttt cggaggttca ccggctgatt tgacagctgc ccgttccgtg gtaaactgtc
      301 cgattttgag gaaggatttt gtgattgacg aatatcagct ttatgaagca aaagtcgtgg
      361 gtgccgatgt gattttgctt attgccgctg cactcacccc cgataagaca aaaaacctgg
      421 cccggcaggc aaaagacctg ggtctggaag tgttgcttga agtgcataac cgcgaagagt
      481 tggactgtgc caatgagttt gtggatttat tgggtgtgaa caaccgaaat ttgaaaacgt
      541 ttgaagtaga tatcaatatc tcaaaagaac tggcagaatt cataccgaaa gaattcataa
      601 aaatcagcga aagcggcata tcaaatcctc agactgtaat cgaactaaaa tcattcggtt
      661 atcagggttt cctgatgggt gaaaatttca tgaaagaaca aaatccggct gatgcactga
      721 aaaggtttat atgcccccta accccctaaa ggggggggga atatttaata gatttaagtc
      781 ttttcttcat atataaaagt tgaaaaaagt attctaaaaa aaatctttaa aatcaacaac
      841 ataaaatcgt tcttctatat ccccccttta gggggttagg gggcagttca agacactatg
      901 caaatcaaaa tctgcggtct gcgtgaccct gaaaacatcc gtgaaattgc tgctttgcaa
      961 ccggatttta tgggttttat cttttatccc aattcaccac gctatgtcgg tcaactggat
     1021 gccggttcgc tgacatcact gccaacgtca ataaaaaaaa cgggtgtatt tgtcaatgaa
     1081 aatcttgaaa atattctaac cgcaatcagt aaatacaacc tggatgctgt gcagcttcat
     1141 ggagcagata acaaaaaatt gtgccggaaa atacgccagg aagccaaaac gatggtaata
     1201 aaggtttttc cgataatgga cgccagcaat tttagagtta ccggcgatta cgaagaggta
     1261 gccgattact ttctgttcga tacgaaaaca gatctgtatg gaggttcggg acagaaattc
     1321 aactggaata ttttacagga atatacaggt aacaagtcgt ttctgttgag cggcggcatt
     1381 ggtgccgatg atgtaaaagc tatcagagct atcgaacatc cgttattggc aggtgtggat
     1441 ctgaacagca aattcgaact gaaaccggga ttaaaaaacg tagctttact taaacaattt
     1501 atcgatgaac t
//
